LOCUS       DANLUQ010000001        83440 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.001, whole genome shotgun sequence.
ACCESSION   DANLUQ010000001 DANLUQ010000000
VERSION     DANLUQ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 83440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 83440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..83440
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.001"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            180..1817
                     /gene="pyrG"
                     /locus_tag="PXB27_000001"
     CDS             180..1817
                     /gene="pyrG"
                     /locus_tag="PXB27_000001"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HDL5344844.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLASILEARGLNVTIMKLDPY
                     INVDPGTMSPTQHGEVFVTEDGAETDLDLGHYERFIRSKMSKANNFTSGKIYSEVLRK
                     ERRGDYLGATIQVIPHITNEIKARVLEGGKGRDVAIVEVGGTVGDIESLPFLEALRQL
                     AVDIGREKTLFMHLTLVPYIPTAGEVKTKPTQHSVKELLSIGIQPDVLICRSDRAIPE
                     NEKKKIALFCNVPERAVISLKDVDSIYRIPELLKSQGLDSFVCDRFRLDCKEADLSEW
                     EQVLYCQANPTGEVTIGMVGKYVELPDAYKSVNEALKHAGLTHRLTVNIKYIDSQDIE
                     TKGTELLAGLDGILVPGGFGYRGVEGKIRTAQYARENKIPYLGICLGMQIALIEYARN
                     VAGLTEANSSEFDKDCSQPVIGLITEWQDAEGNVETRSDESDLGGTMRLGSQQCHLIE
                     GSKARELYGAETIVERHRHRYEVNNKLLPQIEAAGLKVSGLSADRKLVEIIEVPNHPW
                     FIAAQFHPEFTSTPRDGHPLFAGFVKAAKDHQESRKA"
     gene            complement(1985..2581)
                     /gene="nfuA"
                     /locus_tag="PXB27_000002"
     CDS             complement(1985..2581)
                     /gene="nfuA"
                     /locus_tag="PXB27_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HDL5344845.1"
                     /translation="MEEQTIHISISDAAQAHFARLLEQQEEGTNIRIFVVNPGTPGAE
                     CGVSYCPPNAVEETDTEFKFNRFSAFVDDISLPFLDEAEIDYVTDPMGSQLTLKAPNA
                     KMRKVADDAPFIERLDYVIQTQVNPQLASHGGKVTLIEITEDKYAVLQFGGGCNGCSM
                     VDVTLKEGIEKQLLAMFPDELVGVKDITEHQHGEHSYY"
     gene            3028..4386
                     /locus_tag="PXB27_000003"
     CDS             3028..4386
                     /locus_tag="PXB27_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HDL5344846.1"
                     /translation="MITIKKGLDLPIAGKPEQVIHNGNTVTEVAMLGEEYVGMRPSMK
                     VREGDVVKKGQVLFEDKKNPGVVFTAPASGTVTAINRGEKRVLQSVVIKVEGDEQITF
                     ARYEASQLASLTSEQVKQNLVESGLWTAFRTRPFSKVPALDAEPSSIFVNAMDTNPLA
                     ANPEVIIAEHQQAFFDGLTVLSRLFESKKVIHLCGSADSNILNAAQSRQINNVETNRF
                     KGPHPAGLSGTHIHFIDPVGATKQVWYLNYQDVIAIGKLFTTGELFTDRVVALAGPQV
                     KNPRLVRTRLGANLSQLTANELNAGENRVISGSVLSGAIATGVHDYLGRYALQVSALL
                     EGREKAFFGMIAPYSDKYSITRTTLGHFAKKLFNFTTAVHGSERAMVPTGAYERVMPL
                     DIIPTLLLRDLAAGDTDSAQALGCLELDEEDLALCTFVCSGKNNWGPLLRQALDKIEK
                     DG"
     gene            4389..5624
                     /locus_tag="PXB27_000004"
     CDS             4389..5624
                     /locus_tag="PXB27_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HDL5344847.1"
                     /translation="MGLKNLFEKMEPAFHKGGKYEKWYTLFEAAYTIFYTPGTVTRKD
                     AHVRDAIDSKRMMLIVWLALFPAMFYGMYNVGAQGILAALKMGTLTDLVANNWHYSLA
                     DSLGSVADAGWGTKMLLGATYFLPIYLTVFAVGGFWEVVFAMVRKHEINEGFFVTSIL
                     LALIVPPTLPLWQAALATTFGVVVAKEVFGGVGKNFMNPALAGRAFLFFAYPGQISGD
                     AVWVAADGFSGATALSQWAVGGQGALQHVATGETITWMDAFLGNIPGSIGETSTLMLI
                     IGAAIIVFARIASWRIIAGVMIGMAATATLFNVIGSDTNPLFSMPWHWHLVLGGFAIG
                     MFFMATDPVSAAFTNKGKWAYGILIGFMCVLIRVVNPAYPEGMMLAILFANLFAPIFD
                     YLVVQGNIKRRLARVANNG"
     gene            5617..6390
                     /locus_tag="PXB27_000005"
     CDS             5617..6390
                     /locus_tag="PXB27_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HDL5344848.1"
                     /translation="MAKFNKDSVGGTLTVVVLLSLICSLIVAGAAVMLKPTQDIQKQL
                     DKQKNILQAAGLLQKDTNVQETYTKFIEPKIVDLATGDYVEGVTGFDARAAAKDPAQN
                     VVIAPEDDKANIKTRAKYAEVYLVKDESGKTTQVVLPMHGNGLWSIMYGFVAVQPDAN
                     TVNGITYYEQGETAGLGGEIANPNWQKNFVGKKLYNEQNQVALRVGKGASADKDHGVD
                     GLSGATLTSNGVDGSFKYWFGQNGFGPYLAKFKAAAGAN"
     gene            6390..7016
                     /locus_tag="PXB27_000006"
     CDS             6390..7016
                     /locus_tag="PXB27_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005717817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HDL5344849.1"
                     /translation="MADNNNLKKLLLSPIVDNNPIALQILGICSALAVTTQLQTAVVM
                     AIAVSLVTAFSSMFISMIRNYIPNSIRIIVQMAIIASLVILVDQILRAYAYDLSKQLS
                     VFVGLIITNCIVMGRAEAFAMKSGPVESFVDGIGNGLGYGAMLIIVAFLRELIGSGKI
                     FGITILETVQNGGWYQANGLFLLAPSAFFIIGFVIWAIRTWKPEQVEK"
     gene            7018..7614
                     /gene="nqrE"
                     /locus_tag="PXB27_000007"
     CDS             7018..7614
                     /gene="nqrE"
                     /locus_tag="PXB27_000007"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005529392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HDL5344850.1"
                     /translation="MEHLLSIFVKSIFIENMALSFFLGMCTFLAVSKKVSTAFGLGIA
                     VIVVLGIAVPANQLVYTHVLKEGALVEGVDLSFLNFITFIGVIAALVQILEMILDKFF
                     PALYSALGIFLPLITVNCAIFGGVSFMVQREYNFVESVVYGVGAGTGWMLAIVALAGL
                     TEKMKYSDVPAGLRGLGITFITVGLMALGFMSFSGIQL"
     gene            7625..8854
                     /gene="nqrF"
                     /locus_tag="PXB27_000008"
     CDS             7625..8854
                     /gene="nqrF"
                     /locus_tag="PXB27_000008"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HDL5344851.1"
                     /translation="MDSNFIFGIIAFTALVLVLAVIILFAKSKLVDSGDITISINNDP
                     EKGITLPAGGKLLGALASKGIFVSSACGGGGSCGQCKVQVKSGGGEILPTELSHISKK
                     EAKEGWRLACQVNVKSSMDVELPEEIFGVKKWECTVISNDNKATFIKELKLQIPEGEE
                     VPFRAGGYIQIEAEPHTVHYKDFDIPKEYHEDWDKFDLWRYTSKVDEHIIRAYSMASY
                     PEEKGIIMLNVRIATPPPRNPDVPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAE
                     MVFIGGGAGMAPMRSHIFDQLKRLKSKRKMSFWYGARSEREMFYVEDFDGLQAENDNF
                     KWYVALSDPLPGDREDYFRGFIHNVLYENYLKNHEAPEDCEYYMCGPPVMNAAVIGML
                     KSLGVEDENILLDDFGG"
     gene            8966..9400
                     /locus_tag="PXB27_000009"
     CDS             8966..9400
                     /locus_tag="PXB27_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HDL5344852.1"
                     /translation="MEQLDITVLENAFNSLEKTIVSLENQAWFNRQEAIIQDTLIAGA
                     IQKFEFVYELSIKMLKRQLKNIESNDTDIDSTEFRDILRMAAQYGLIESPEVWLDYRK
                     MRNITSHTYDQNKAQQVYNGISNFLESTYFLVKQLQKRNSYD"
     gene            9393..9686
                     /locus_tag="PXB27_000010"
     CDS             9393..9686
                     /locus_tag="PXB27_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDL5344853.1"
                     /translation="MIDLTESQRQQVLDIFKKYLPDTPVWVFGSRIKGTAKAYSDLDL
                     ALITKQPLTIRQQTELELAFTDSDLPFTVDLIDWASCNESFKQIILQRYEVLI"
     gene            9735..10769
                     /locus_tag="PXB27_000011"
     CDS             9735..10769
                     /locus_tag="PXB27_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDL5344854.1"
                     /translation="MKKFFLLLIAFFTLSACEKAPEQITLSGKTMGTTYTVKYIDNGE
                     VENLISPEKMKNELDGLLIEVNNQMSTYQQDSEISRFNQAKQANQAVEISQDFAKVVQ
                     EAIRLNKVTEGALDVTVGPLVNLWGFGPDKRLNKVPSDEQIAERSAYVGIDKILLEMS
                     SKPKLTKSAANLYLDLSSIAKGFGVDKLAEHLETIGVSNYLVEIGGELRGKGKNLQGL
                     DWRIAIEKPVMTQGQAAQITVPLHNLAMATSGNYRNYFEDEKGNRLSHIIDPKQLRPV
                     NHNLASITVFAPTTMTADGLSTGLFVLGAEKALAVAEREKLAIFLIVKNGDKFETQMS
                     SEFSKLIQSQ"
     gene            10782..11027
                     /gene="nqrM"
                     /locus_tag="PXB27_000012"
     CDS             10782..11027
                     /gene="nqrM"
                     /locus_tag="PXB27_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HDL5344855.1"
                     /translation="METVLLTFGFFVAVIFAMSIGFIVKGKTIKGSCGGITALGMKKM
                     CDCEEPCDNLKSKIADGTADPEEVARFNKEPQFYEVK"
     gene            11029..11445
                     /locus_tag="PXB27_000013"
     CDS             11029..11445
                     /locus_tag="PXB27_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HDL5344856.1"
                     /translation="MNRDIRWKQRFDNYKKSVSYLQAEAEKYADTDIDVIKKGIIQSF
                     EITHELAWKLMQDILKWEGEVDIYGSKTASRLAFNRGLITQGEVWLEMIKSRNITVHT
                     DDETILTEEFNKIIKAYLPLFIEFEQRVETLCQTLD"
     gene            11427..11738
                     /locus_tag="PXB27_000014"
     CDS             11427..11738
                     /locus_tag="PXB27_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDL5344857.1"
                     /translation="MPNFGLSERATKTITDILAKYPEVEQAIIYGSRAKGNYREGSDV
                     DLTFKGKDLTYDIVKRIWGDLDDSDSPYLFDLSIYHQLTNANFIEHIDRVGKVFYQRE
                     L"
     gene            11849..13012
                     /gene="mnmA"
                     /locus_tag="PXB27_000015"
     CDS             11849..13012
                     /gene="mnmA"
                     /locus_tag="PXB27_000015"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDL5344858.1"
                     /translation="MTTQLTSKTYDTHFPKLTPEQLAENSKKKVIIGMSGGVDSSVSA
                     FILQQQGYQVEGLFMKNWEEDDDTDYCTAAAELADAQAVADKLGMKLHKINFAAEYWD
                     NVFEYFLAEYKAGRTPNPDILCNKEIKFKAFLEYAAEDLGADYIATGHYVRRTSDDQD
                     AKLLRGLDENKDQSYFLYALNKKQIGQSLFPVGEIEKPIVRAIAEDLGLATAKKKDST
                     GICFIGERKFKDFLARFLPAQPGEIRTVEGKLVGRHDGLMYHTLGQRKGLGIGGVKGL
                     SEDPFYVVEKDLINNVLIVAQGHDNSALLSSGLIAKQLTWVDMQPLRENLRCTVKTRY
                     RQADIACEIQVIDDETIRVMFDEPQIAVTPGQSAVFYLGEVCLGGGIIESQLK"
     gene            13129..14508
                     /gene="argH"
                     /locus_tag="PXB27_000016"
     CDS             13129..14508
                     /gene="argH"
                     /locus_tag="PXB27_000016"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDL5344859.1"
                     /translation="MALWGGRFTQQADAKFKYFNDSLRFDYRLAIQDIEGSIGWAKAI
                     TSVGILTVAELEQLIAALKEVRAEVESNLTIILKDDAEDIHSWVESKLIEKVGDLGKK
                     LHTGRSRNDQVAVDIKMWCKVQAVALQERIRALQERLVSVAEENQESVMPGYTHLQRA
                     QPVTFAHWCMAYYEMLERDFSRLSDANKRMSTCPLGSGALAGTAYGIDRDLLARDLGF
                     EEATRNSLDSVSDRDHILELLSTASISMMHLSRFAEDLIIFNSGESGFLEMSDRVTSG
                     SSLMPQKKNPDACELIRGKSGRVFGALNGLLTTLKGLPLAYNKDMQEDKEGIFDALET
                     WQACLEIAELVLVDIQVNTERTREAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VYAISKKEPLEALSINEFQQFHHSIGDDVYPILSLESCLEKRCAKGGVNPQRVAEAIA
                     VAKANLASK"
     gene            14676..15674
                     /locus_tag="PXB27_000017"
     CDS             14676..15674
                     /locus_tag="PXB27_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HDL5344860.1"
                     /translation="MTESVNSTQAGTALSLGQQLKAAREALNLSITDVAQKTNLKKSH
                     IESLENDIFILQNVAPTFVRGYVRNYVKFLRLPESLISSVNYGEVTIPKEVTKVSSVK
                     PSSNSQGKTLKYLTVLVLLAALGMTLLWWWQGYQKEQENREQFVSSAPVAEVANTATN
                     NDASAQTSVPVQQPIQVSEQPAQVAETVVAAEPALTEKVEPTQAASSPVTQEDVKVVE
                     EVKQADATTAQVEAVNVLQQSQSEQAPTEQPVAVTNDELRIEITGSQSWITVRGAKNK
                     RLAEKLYNNGETLSFNENEQYRLTIGAPANVKVYYKGQEVPLKIDGRVARIHLPLQ"
     gene            15775..16887
                     /gene="ispG"
                     /locus_tag="PXB27_000018"
                     /gene_synonym="gcpE"
     CDS             15775..16887
                     /gene="ispG"
                     /locus_tag="PXB27_000018"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HDL5344861.1"
                     /translation="MIHQSPIKRRVSKKIYVGNVPVGGDAPITVQSMTNTRTTDVEAT
                     VAQIKSLERVGADIVRVSVPTMDAAEAFKAIKQQVSVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRTVVDCARDKNIPIRIGVNAGSLERDLQEKYGEPTPQALLES
                     ALRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARAGSV
                     KSAVGLGLLLAEGIGDTLRVSLAADPVEEVKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EIDVIATVNELEQRLEDIITPMDVSIIGCVVNGPGEALVSDLGVTGSNKMSGFYLDGV
                     RQKERFDNAKLIDQLEAKIRARVAQQHNRIDIEQIG"
     gene            16893..18164
                     /gene="hisS"
                     /locus_tag="PXB27_000019"
     CDS             16893..18164
                     /gene="hisS"
                     /locus_tag="PXB27_000019"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDL5344862.1"
                     /translation="MAKTIQAIRGMNDCSPSESPLWQWVEQKVRNVLASYGYSEVRMP
                     IVESTPLFARAIGEVTDVVSKEMYTFWDNDEQLTLRPEGTAGCVRAAIERGWIYNNEQ
                     RLWYMGPMFRHERPQKGRYRQFHQAGVEVFGIANPEIDAELIILTARLWKELGIDQHV
                     SLQLNSIGSLEARANYRSALVKFLENHTALMNEEEKERLVKNPLRILDTKNEALQNVL
                     NDAPKLLDYLDDDSREHFTQLCALLDAMGIRYEVNPKLVRGLDYYNKTVFEWVTSALG
                     SQGTVCGGGRYDGLVEQLGGHATQGVGFAMGLERLVLLVQEVNKEIDLPKTVDVYIVF
                     SGEGATLNAFQLAEQIRSELPHVGVMTHCSGGKFQKQFKRADKVEAQIALVIGESEVA
                     EKQVVIKDLRSGAEQITIAQSELIAELSKRF"
     gene            18174..18794
                     /locus_tag="PXB27_000020"
     CDS             18174..18794
                     /locus_tag="PXB27_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDL5344863.1"
                     /translation="MSNEYLNKTEEQQFEDAKNWFKENGTPILVAIILACAATAGWNF
                     WKNHQLETAQKTSASYQSVMESYLQNPSKNQPLVEKFIADNKDSSYAAFAQFEVAKQA
                     VEKGNFSAAKTALQSVLTYSDSTLQSIARFRLAQIDFQLKSYDEALASLAQIQDKAWE
                     LRKQILTGDILAAKGDIPAAKSAYEQAKTTANPQEQTLIDVRLNNL"
     gene            complement(18857..19960)
                     /gene="dnaN"
                     /locus_tag="PXB27_000021"
     CDS             complement(18857..19960)
                     /gene="dnaN"
                     /locus_tag="PXB27_000021"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDL5344864.1"
                     /translation="MQITITREQLLKPLQQVCGVLSSRPTLPVINNLLLEIEGNRLSL
                     TGTDLEVELTTQAQLLQAVDFAGKFTIPAKKFLDISKSLPDDSVISVQFEEDKAIIKA
                     GRSKFSLATLPASDYPNLMDWKPEVDFSIDQSTLARLIDATQFSMANQDARYFLNGMK
                     FETEGNLLRTVATDGHRLAVCTMVLNQELLTHSVIVPRKAVLELSRLVASNDETVRLE
                     IGSSNIRITMNGIVFTSKLIDGRFPDYRRVLPRNADRILEAETETLKRALMRAAILSN
                     EKFRGVRLTLSNNLLKITANNPEQEEAEEIIDVAYDSPEMEVGFNVSYVLDVLNTLKC
                     QRVRFSLVDSSSSCLIEDCDNSSAEYVIMPMRL"
     gene            complement(19979..21328)
                     /gene="dnaA"
                     /locus_tag="PXB27_000022"
     CDS             complement(19979..21328)
                     /gene="dnaA"
                     /locus_tag="PXB27_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDL5344865.1"
                     /translation="MEQIVSSLWSDCLNHLQTKVSPTDYSTWLRPLQASSENGELTLY
                     AQNQFVENWVKDKFLSEITELARFLAKNDNLVVTLKVGIKPTEQPKVSPNLKNESSEF
                     SNTGFKTGLNPNQTFDNFVQGRSNQLAKLVAQQVVDNLGESHCNPLSFYAGTGLGKTH
                     LLHAIGNELVKRTPGTRVLYIHLERFYRDIIKAINSSNNDAEKIKKFYRSLDVLMIDD
                     IQFLANKPKVQEEFLHLLNALFEQRKQIILTSDVLPKNIDKIDTAIKSRLSGGISATI
                     EPPELETRVAILRQKALERGADLQEDAAYLMGQKLRTNVRELEGALNRVIAWQKLTLR
                     PITVDAVRETLQDLFASFDYLITIENIQRIVAEYYGIKVADIKSKSRARSIARPRQMA
                     MALAKELTNHSLPEIGREFGGRDHTTVMHACKTINELRDTESGIQEDYIHLTRKLSS"
     gene            complement(21515..23362)
                     /locus_tag="PXB27_000023"
     CDS             complement(21515..23362)
                     /locus_tag="PXB27_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5344866.1"
                     /translation="MFEKLFKWFEARVETYPNEMPKTPKSGLIPFIYDATKGMRFYIL
                     LLTVLVAAVGIIEAVLFRFMGDLVDWVNKYSPTELWAEKGWTIIGMFFIALLAVVFVY
                     LASSIRFQSLQGVFPMRLRWNFHRLMLGQSLGFYQDEFAGRVSAKVMQTALSVRDVIM
                     TCADMLVYVLVYFTTSSVILLQLDGWLFVPFILWVISLAATIIYFVPKLAQAAQEQSD
                     ARSLMTGRITDAYANIATVKLFSHGNRESAYAKESMEGFMVTVHKQMRLVTVIETVTN
                     LTSMALIISTAGIGLWLWGSSVVTAGAIATSTALALRIKGLSQWIMWEFARLFENLGT
                     VQDGMITLSKPHNVVDKIDAKPLNVTKGEIKFENVDFAYDPKKPLLKDFDLTIKPGEK
                     VGLVGRSGAGKSTLTNLLLRFYDIQDGSITIDGQNIRDITQESLRSQIGLVTQDTSLL
                     HRSVRENLMYGRPNATEEEMIKAVEQAAASEFIPNLQDAKGRTGFDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEVAIQENLTQLMEGKTVVAIAHRLST
                     IMAMDRLIVLDKGEIVEQGTHEELLAQNGVYARLWAHQSGGFLPENTDI"
     gene            complement(23431..23940)
                     /gene="luxS"
                     /locus_tag="PXB27_000024"
     CDS             complement(23431..23940)
                     /gene="luxS"
                     /locus_tag="PXB27_000024"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDL5344867.1"
                     /translation="MPLLDSFKVDHTKMNAPAVRVAKTMTTPKGDTITVFDLRFCRPN
                     IDILPVRGIHTMEHLFAGFMRDHLNSEQVEIIDISPMGCRTGFYMSLIGSPSAEEVVK
                     AWTASMEDALHKIPDVSAIPELNEYQCGSYKEHSLKEAHQIARNVLAAGIGINRNEDL
                     ALDEKLLNP"
     gene            complement(23958..26624)
                     /locus_tag="PXB27_000025"
     CDS             complement(23958..26624)
                     /locus_tag="PXB27_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HDL5344868.1"
                     /translation="MTMEENTHTVVDAQIRQPRKISPFWLLPIVAFIIGALLFFQILK
                     EQGETITIRFSKGDGITAGKTAIRYQGLQIGQVKRVYFVDNLKEVEVQAEINPEAKSI
                     LRKQTKFWLVQPSASLAGVSGLDAIVSGNYITLLPGEGDFVDEFIAEEDPPAITVSDG
                     DLLIRLISDDIGSITVGASVYFRKVPVGNIADYRFTKDQKKIEIDVVINQKYAHLVKK
                     ESRFWNISGISADVNLQGIKINMDSLASVVQGAVAFDSPDNMEIAEQGQKFELYPDLK
                     SAKRGIEINVKLPLTHNLKINETGVFHQNVQIGMLSQLKLPQVKDNGVPQQESGQHKQ
                     IDGVLLIDPSYENLLRSQTKILLKEPKFSLNTEQLTKVGELLRGVYFEVHPGEGEPQS
                     EFVVQNEADYLLSLPNLLSFSLIAPQAYGVDTNQGIYYSDVQIGEIVKRSLELDKVRF
                     DVIIYPPYRNLIGSNSKFVAISNIDMSVGLDGLRVHAGSPSEWLKGGVRLINGKNDGS
                     PKKQYALYKDIESAESGMATLDKKATTTLSADSLSGISEGSLVLYRDFQVGEVLKITP
                     KQQKFEVELFIQPNYRHLLTEKSRFWIEPATSVDVSLKGVNITTAPLMRTLKGAISFD
                     DGGSKGNKTLYASKEKATSGNTYLTLIAKDASKLSEGMDIKYMGLTVGQIEKLELQNA
                     KKQIKATAYIQRKYYPLIAKAGSKFNVISPEISTSGFKNLDAALQNYITVDVGSGQAQ
                     NQFVLADTDTVKTTYANGFPIIVETTNANGIQPEAPVLYRGMQVGIVSRLGLSELGDR
                     VLIYVNIQDKYKHLVRTNTQFWQASGYTMDVSLQGVSMNSGTMSQLLNGGIEFSTPYT
                     KVVKPQAQPNRRFFLQRKLPDEAPAWDQGIAE"
     gene            complement(26707..27147)
                     /gene="fur"
                     /locus_tag="PXB27_000026"
     CDS             complement(26707..27147)
                     /gene="fur"
                     /locus_tag="PXB27_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HDL5344869.1"
                     /translation="MSEANIKRLKEVGLKVTEPRLTILALMQDIRDEMQHFAAEDIYK
                     ILLEKGSDIGLATVYRVLNQFEEAGILTRHNFDANKAVFELNMNHEHDHIICMDCGKV
                     FEFKDPDMERRQREISEKHGMELTNHSLYLYGKCSNLTNCDEKK"
     gene            complement(27183..27707)
                     /gene="fldA"
                     /locus_tag="PXB27_000027"
     CDS             complement(27183..27707)
                     /gene="fldA"
                     /locus_tag="PXB27_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HDL5344870.1"
                     /translation="MAVVGLFYGSDTGNTENISKMIQKKLGAELIDIRDIAKSTKEDI
                     EAYDFLMFGIPTWYYGESQADWDDFMPTLKEIDFNGKVVAIFGCGDQEDYAEYFCDAM
                     GTVRDVVEPNGGVIVGHWPTAGYTFEVSQALVDDDTFVGLCIDEDRQPELTTERVNKW
                     CEQLKEEMCLDQLA"
     gene            complement(27713..27988)
                     /gene="ybfE"
                     /locus_tag="PXB27_000028"
     CDS             complement(27713..27988)
                     /gene="ybfE"
                     /locus_tag="PXB27_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HDL5344871.1"
                     /translation="MAKQDSDCITLDLFATVPKVGRPRTNPLDREQQLRINKRNQLKR
                     DKSSGLKRVELKLHSNLVKLLEEQASEQGVSRGQLIETILNNYLTNR"
     gene            28229..29215
                     /locus_tag="PXB27_000029"
     CDS             28229..29215
                     /locus_tag="PXB27_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDL5344872.1"
                     /translation="MSNIHLAIAADFTLAEKLLEALANQDLGIENISAVELEPFGEEQ
                     NLRFGAKAVAQYALDEVNWAEFSHVLFAGKMAQAEVLAEAVKAGCVILDLYGITALIG
                     DVPVVVPSVNDENVANLRERNIVALANPQISQLALALKPLLNEPLKQVWVTSLLPSAY
                     FGDEKVKELAGQTARLLNGIPFDENAERIAFDVVPANVQGEEKSLPFSKAFELQIAKV
                     LPNLTACTTFHSVQVPVFYGVSQMVSVQSEYQLDANALSEAWKAENWLNFNENSVITA
                     VKNGENEEQNALEISQLLAKSEQEVQFWTVADEQRFSLAFLAVKLLKLVLAY"
     gene            29312..30607
                     /locus_tag="PXB27_000030"
     CDS             29312..30607
                     /locus_tag="PXB27_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL5344873.1"
                     /translation="MLEKLFQLKAKGSNVKTEIIAGITTFFTMVYIVFVNPSILGVAG
                     MDTQVVFVTTCLIAAFGTIAMGLFSNLPIALAPAMGLNAFFAFVVVQKLGYSWQVGMG
                     AIFLGSVGLFLLTVLQVRYWFMSAIPLGLRVGIGAGIGLFIALIGFKNMGLVVPNPAT
                     LVALGDLHDPKVLMGILGFFIIVVLAAKGIHSGVLISIVIVTALALAFDPAVSFNGIM
                     SMPPSLGAVVGQVDIAGAFDVALLGMIFSFMLVNLFDSSGTLIAVTTKAGFADEQGRF
                     PKMKQALLVDSTAAMAGSYMGTSAISTYIESGSGVSVGGRTGLTAVVVGILFLLTIFF
                     SPLAGLVPAYATAGALVFVGILMASSLIEVKWDDLTEATPAFITTAMMPFTYSITEGI
                     AFGFISYCVMKAFTGRWKEINLSVLVVSLLFIAKFVWVG"
     gene            30727..31095
                     /locus_tag="PXB27_000031"
     CDS             30727..31095
                     /locus_tag="PXB27_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/TusD sulfur relay family protein"
                     /protein_id="HDL5344874.1"
                     /translation="MSNSVQKLLFIINSAPYGNETFFSALRLALQIQEQHKANTEIKL
                     FLMSDAVTAGLAKQNPAEGYHIQQMLEILTAQGANVKLCKTCTNARGITDLPLADGVE
                     IGTLIELADWTIQADKVLNF"
     gene            31095..32288
                     /locus_tag="PXB27_000032"
     CDS             31095..32288
                     /locus_tag="PXB27_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL5344875.1"
                     /translation="MQNIDVVVIGAGAAGLFCAAQLGQAGKKVVVLDSGKKVGRKILM
                     SGGGFCNFTNLDVSAGNYLSQNRHFVKSALSRYTQWDFISLVSHYGISYHEKELGQLF
                     CDESSQQIVDLLQAECNKGKVEILLRQAVISVEKVANFFEISTACGKFQSEHLVIATG
                     GLSMPALGASPFGYQLAEQFGIPVVPPRASLVPFTWKESDKYFAELSGIALPITVSAN
                     GKSFSNQMLFTHRGLSGPAILQISNYWELGETVEIDLLPSQSITDILSELRQSSPKLQ
                     LKTVLSRYLPKKLVEVWLEQKLLTDKVIADLSKKDLTLLEQFIHHWHFLPNGTEGYRT
                     AEVTKGGVDTDYISSKTMEAKNTAGLYFIGEVLDVTGWLGGYNFQWAWSSAFACAEGI
                     IEKSK"
     gene            complement(32359..32577)
                     /gene="infA"
                     /locus_tag="PXB27_000033"
     CDS             complement(32359..32577)
                     /gene="infA"
                     /locus_tag="PXB27_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDL5344876.1"
                     /translation="MAKEDCIEMQGTILETLPNTMFRVELENGHIVTAHISGKMRKNY
                     IRILTGDKVTVEMTPYDLSKARIIFRAR"
     gene            complement(32687..33346)
                     /locus_tag="PXB27_000034"
     CDS             complement(32687..33346)
                     /locus_tag="PXB27_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HDL5344877.1"
                     /translation="MYISREKITKTLLLAGITLLCCGIVAVILMGVNQFKLGSQLASI
                     NQVSNLSHLLVRQQANIFSFLLVKNAKTDELVDALDSFAKEDFIIDANIYSPNGVLLA
                     QSQNAKDLKVLLDSEQSAKTQQIVEPIFSQQALVGFLRVTFDAEYGQTTKARVEQQFH
                     QLYGEIIILLLTGILLASSINYYFRRKIHHVQISPKPLMSQVKTQTQRFHSRRRWIKR
                     K"
     gene            33432..34295
                     /gene="serB"
                     /locus_tag="PXB27_000035"
     CDS             33432..34295
                     /gene="serB"
                     /locus_tag="PXB27_000035"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HDL5344878.1"
                     /translation="MTNTIFIYSKTLNEREIEQFSQQTAAILLKQAVYLDYKIAFFST
                     NLTACELRETAQKINADISDLTIIPTLNQGGLLLMDMDSTAIKIECIDDIAKLAGTGE
                     VVSAITASAMRGELDFEQSLRKRVGTLENASESILQKVRENLPLMDGFEQMVAILKEH
                     GWKLAIASGGFDYFADYLKQKYGLNFAVSNQLEIIDGKLTGKVLGKVVDAQHKADTLQ
                     NLATTFNIPPNQWVAVGDGANDLPMLKTASLGVALHAKPKVQAQADFVVNFGDLTALC
                     LLLNANHLFQH"
     gene            34311..34802
                     /locus_tag="PXB27_000036"
     CDS             34311..34802
                     /locus_tag="PXB27_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HDL5344879.1"
                     /translation="MPSFDIASEITMHEVRNAVENANRVLSTRYDFRGVEAVIELNEK
                     NESIKLTTESDFQLEQLIEILIGSCVKRGIEHSSLDIPTESEHHGKLYSKEIKLKQGI
                     ESDMAKKITKLIKDSKIKVQAQIQGEQVRVTGKSRDDLQAAIQLVKGADLGQPFQFNN
                     FRD"
     gene            34887..35063
                     /locus_tag="PXB27_000037"
     CDS             34887..35063
                     /locus_tag="PXB27_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5363 domain-containing protein"
                     /protein_id="HDL5344880.1"
                     /translation="MMSEQTEKKGWFKKALEKYDAFCDELGVNQGACRGCVPVVKFDP
                     EPEKEQPEKSENKT"
     gene            complement(35111..35407)
                     /locus_tag="PXB27_000038"
     CDS             complement(35111..35407)
                     /locus_tag="PXB27_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5344881.1"
                     /translation="MKKHHIIILTLASASVVACSSNTPVQVVNGDKNIDYSQPKTIYI
                     SETNTQPPALTKQEKIKPSNATALCFNGSYSTDIQNPCKKDGGVKQYFAHHHAN"
     gene            complement(35484..36452)
                     /gene="mdh"
                     /locus_tag="PXB27_000039"
     CDS             complement(35484..36452)
                     /gene="mdh"
                     /locus_tag="PXB27_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HDL5344882.1"
                     /translation="MCQSKKVALLGAAGGIGQSLALLLKLNLPAKSELSLYDISPVTP
                     GIAVDLSHIPTDVKVTGFAGEDPTEALKDADVVVISAGVARKPGMTRADLFNTNATIV
                     HNLVEKAAKVCPKACIAIITNPVNTIIPIAAEVLKKAGVYDKNKLFGVTTLDVIRANT
                     FVAEAKDVNVKYVRVPVIGGHSGTTILPLLSQATVNGLKLEFTQEQIEQLTHRIQNAG
                     TEVVEAKAGGGSATLSMAQAGAEFALGLVRALIGEDVIRYAYVDNANGETSPAFFAYP
                     IRLGTNGVEKVLPIGNLSEFEKDQLEQLIPVLNDEIQLGQTFNKNA"
     gene            36648..37109
                     /gene="argR"
                     /locus_tag="PXB27_000040"
     CDS             36648..37109
                     /gene="argR"
                     /locus_tag="PXB27_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HDL5344883.1"
                     /translation="MDKIDSLSEAFKSLLKEEKFGSQSEIVAALQEMGFDNINQSKVS
                     RMLSKFGAVRTRNPKMEMVYQLPVELSVPTTSSPLKNLVVDIDHNDALIVVKTSPGAA
                     QLIARLLDSMGKAEGILGTIAGDDTIFITPVRNVQIEKLVKNVTQLFETSL"
     gene            37159..38055
                     /locus_tag="PXB27_000041"
     CDS             37159..38055
                     /locus_tag="PXB27_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HDL5344884.1"
                     /translation="MHIFITGGTGFIGRELIQQLKSENHHFTLLTRDTEAKSDDLQIN
                     YCYSLDELVDLNDFDAVINLAGEPIFDKRWTDEQKQKLIESRVNLTNKLSQLMNASSN
                     PPHTFLSGSAIGIYGDLPPSANFYTESTACYQDCFTNRLCQQWEAAALKAASDKTRVC
                     LIRTGTVFSPEGGALKKILPLYRCGLGGKLGSGKQYWAWISRRDHVQAVIFLLKNANC
                     SGAYNFVAPNPVRNEEFNRILANAVNRPAFFTVPEFALKLGLGERACLVLDNQQLIPE
                     KLLAASFVFQDPILTENVFLNP"
     gene            38100..40298
                     /gene="uvrD"
                     /locus_tag="PXB27_000042"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             38100..40298
                     /gene="uvrD"
                     /locus_tag="PXB27_000042"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HDL5344885.1"
                     /translation="MDFSLLLDGLNDKQREAVAAPIGNYLVLAGAGSGKTRVLTHRIA
                     WLIGVENVPESNILAVTFTNKAAAEMRHRIEYTLSQSGDNRLFGMWVGTFHSIANRLL
                     RSHYLDADLPQDFQIMDTEDQQRLLKRLLKLHNIDEKHFPPKHVAWYINAQKDKGKRA
                     KDIDHYNDPNEKKLVEIYQIYQDACDRAGLLDFAELLIRAYELFKTKSTILQRYQQRF
                     QQILIDEFQDTNNIQYDLIRLLAGDVGKVMIVGDDDQSIYGWRGAQVENIQRFLNDYD
                     NAQTIRLEQNYRSTGNILSCANELIANNDNRLGKNLWTDSGDGEPVDIYCAFNELDEA
                     RFVASQIKQWKEDEGDLAECAVLYRSNSQSRVIEEALIQANIPYRIYGGMRFFERQEI
                     KDALAYLRLIANRQDDAAFERVINTPTRGIGDRTLDTLRQITRGRQITLWQAIQVAVQ
                     EEQLSGRATSSLLRFVELINALDQETEQMSLAEQTDFVIKKSGLYEMYKQEKGEKGEV
                     RIENLEELVTATKQFVKPDEAEELTDLTAFLTHASLEAGEAQASQHEDYVELMTLHSA
                     KGLEFPRVFIVGVEEGIFPSGMSFDEGRLQEERRLAYVGITRAKKKLTISYAESRRLY
                     GKEERHVPSRFIAELPEEHIREVRLRGSINRAASFNQANSFAKNPTKTTASFLNDDTW
                     KMGQKVNHAKFGQGTIINVEGSGEATRLQVAFVGNGIKWLIAKMANLERI"
     gene            complement(40445..40519)
                     /locus_tag="PXB27_000043"
     tRNA            complement(40445..40519)
                     /locus_tag="PXB27_000043"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40485..40487),aa:Gln,seq:ttg)
     gene            complement(40574..40648)
                     /locus_tag="PXB27_000044"
     tRNA            complement(40574..40648)
                     /locus_tag="PXB27_000044"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40614..40616),aa:Gln,seq:ttg)
     gene            complement(40674..40758)
                     /locus_tag="PXB27_000045"
     tRNA            complement(40674..40758)
                     /locus_tag="PXB27_000045"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40722..40724),aa:Leu,seq:tag)
     gene            complement(40761..40837)
                     /locus_tag="PXB27_000046"
     tRNA            complement(40761..40837)
                     /locus_tag="PXB27_000046"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40801..40803),aa:Met,seq:cat)
     gene            complement(41004..41828)
                     /gene="aroE"
                     /locus_tag="PXB27_000047"
     CDS             complement(41004..41828)
                     /gene="aroE"
                     /locus_tag="PXB27_000047"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005615478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDL5344886.1"
                     /translation="MNRYAVWGNPIAQSKSPRIHQLFALQTQRNIEYIANLGDETAFE
                     QQLADFFASGAKGCNITAPFKERAFKLADEFSETALLAQACNTLKRLEDGRLYADNTD
                     GAGLVSDLERLGWLQKGHKTLILGAGGATKGVLLPLLKAGLEITLYNRTQEKAVVLAE
                     KFAKFGKIQTACLSDLANQLFNLIVNATSLGLQGKYVELPESVIASANIYDMQYAPNM
                     QTPFLNYARRCGATACQDGLGMLVGQAAFAFSLWEGQFPDVEPVLAQLKLEMQTVK"
     gene            complement(41835..42389)
                     /locus_tag="PXB27_000048"
     CDS             complement(41835..42389)
                     /locus_tag="PXB27_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HDL5344887.1"
                     /translation="MNNFLEIVNQLKQEEVVAYPTEAVFGLGCNPQSEKAVRKLLVLK
                     QRPEEKGLILLASDLKYLLPYIDEARMTEQAWQRLSHIGEQAITWIVPVKNTVPSYLT
                     GKFDSIAVRLCNVPAVVQLCEASGVALTSTSANLSGEPPCRNAKEVIDQFGQDFPVLH
                     ADTLGRENPSEIRDIFSQQIFRQG"
     gene            complement(42382..42918)
                     /locus_tag="PXB27_000049"
     CDS             complement(42382..42918)
                     /locus_tag="PXB27_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HDL5344888.1"
                     /translation="MVLFKSTKQTETCPQCGSPLQIKQGKKGLFLGCSAYPECDYIKP
                     LHQAGHIIKQLDELCPECGGNLQLKQGNYGIFIGCSHYPECHFIVHEEAEVEEEFDCP
                     ECKQHKLIARKGRSGKIFYGCIGFPNCKFTLATRPINHLCEKCGCELATIKKIKGKQF
                     TICANKSCQHQTELKPNE"
     gene            complement(42928..43239)
                     /locus_tag="PXB27_000050"
     CDS             complement(42928..43239)
                     /locus_tag="PXB27_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005724113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDL5344889.1"
                     /translation="MNREKITFFSRFEADILSGKKTITIRDKSESYFQPNQELAVFTN
                     ETDRFFANIKVLSVTPIVFDELNEQHAKQENMSLAELKQVIREIYPNDNAFFVIEFTL
                     I"
     gene            complement(43232..43708)
                     /gene="recX"
                     /locus_tag="PXB27_000051"
     CDS             complement(43232..43708)
                     /gene="recX"
                     /locus_tag="PXB27_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDL5344890.1"
                     /translation="MTTSTTKTNKYTAVNYLLYLLSKRDYSEQELRQKLKQKEYELTE
                     IDAAIEKAQANQWQSDERFCTTFIRYRSMQGIGPRRLKQELKLKGVKDWLINQELENA
                     EVDWFELAEQIFEKKRPLVWDLKAKQKMWRFMVSRGFYNDHFSHLMALDYSSDEYE"
     gene            complement(43767..44873)
                     /gene="recA"
                     /locus_tag="PXB27_000052"
     CDS             complement(43767..44873)
                     /gene="recA"
                     /locus_tag="PXB27_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDL5344891.1"
                     /translation="MAEKKSQKNTPVKQIDPEQKEKALAAALAQIEKQFGKGSIMKLG
                     DTQALDIEAVSTGSLGLDSALGIGGLPMGRIVEIYGPESSGKTTLTLSVVAQAQKNGK
                     TCAFIDAEHALDPIYARKLGVDTDGLLISQPDNGEQALEICDALVRSGAVDVIIVDSV
                     AALTPKAEIEGDMGDSHMGLQARLMSQALRKLTANIKATNCLVIFINQIRMKIGVMFG
                     NPETTTGGNALKFYASVRLDIRRSGVVKDGDEVIGSETKVKIVKNKVAPPFREVQFDI
                     MYGEGIARMNELLILAESHGFINKAGAWFSYEGEKIGQGKNNAIKWLKEHPEVASKIE
                     QDIRNLLISNPTFTATPDSENADNADDEFSEEEL"
     gene            complement(44974..45594)
                     /locus_tag="PXB27_000053"
     CDS             complement(44974..45594)
                     /locus_tag="PXB27_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HDL5344892.1"
                     /translation="MCRFPLFETLSIIDGKVQNLQYHQQRFEQATQEYFGCEPRFSLA
                     DILVVPEASQRGKVRCRIDYNATEFELKFFIYSPKKVERFRLVEVENWDYHLKFSDRK
                     RFDLLPILQNEEVIILNNGYVSDCSIGNLLFLKQGIWYSPQHYLLKGTQLSKLLDEGK
                     VVLAKIAKQDLHQYEKIMLINALNPFEEPRALPCSQIYFCDLDRKS"
     gene            complement(45572..46546)
                     /locus_tag="PXB27_000054"
     CDS             complement(45572..46546)
                     /locus_tag="PXB27_000054"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HDL5344893.1"
                     /translation="MQDFIHQANHYGQAKRPFFFLIDFEQQKPLIFPLEQAHASGLFF
                     EFFCKSNLEKPVEKPTKAFELSANPIPFSEYQTGFELVKKEIQAGNSYLLNLSYPTAI
                     ETNYSLAEIFAASKAKYKCYLQGRFVCFSPECFVRIENNRIFSYPMKGTINANEENAE
                     QKLMASEKEFTEHNTIVDLIRNDLALVAKNIEVTKYRYVEKVETHRGAILQTSSEICG
                     ELGENWQENIGSILAKLLPAGSISGAPKEKTVSIIQQAELGKRGYYTGIFGYFDGESL
                     ESAVAIRYIAQTSNGLVFHSGGGITSQSVLTDEYNELLEKVYVPISAI"
     gene            46656..48263
                     /gene="rmuC"
                     /locus_tag="PXB27_000055"
     CDS             46656..48263
                     /gene="rmuC"
                     /locus_tag="PXB27_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HDL5344894.1"
                     /translation="MHFQEIGIYIALAVCVLVGVYLFFLKSRYQRNAYELSQDLGKIT
                     ENFEQIQQKYEVLAQEKNQLEQWAIQQQTKYEAVSERLNERDGQLQRFQQKIELAEQQ
                     ENQLERYINELKERVGSSQAKAESLEEQLQFSQSSLSTKERENQALFSRLNEVQNELT
                     ELRTTLSEKQANFEAQQRNFIEVKQQLNVEFQHLAQQILDEKSKRFSETNQSSLEALL
                     KPFKEQIEGFQKRVNEVHSESLKGSANLEAEIKRVLQIGVSMSEEAQNLATALKGNNK
                     IAGNWGEVQLESALQSAGLLAGEHYQAQESFRDEEGRRFAPDFVVHLPDQKHLIIDSK
                     VSLVAYDQAVRSEENFAISQALDEHCRSLRNHIEGLSKKNYSALLGIKSPDFVLMFIP
                     IEPAYIEAMKHDPQLFNFGYERNVILVSHTTLMPILRTVANLWRIERGNSEAREISEK
                     AGEIYNQVCAVADRLAKLGNTLNTANNQYNQTVTSLVGRQGLLGKVERFQHLSSKASQ
                     IVPQVELIENEVDTIKLEMVSRELLEE"
     gene            48326..48910
                     /locus_tag="PXB27_000056"
     CDS             48326..48910
                     /locus_tag="PXB27_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component II"
                     /protein_id="HDL5344895.1"
                     /translation="MLKKLLIIDNHDSFTFNLVDLLRKLDVPAKVVLVEELDLNEVEH
                     FSHILISPGPDVPRAYLQTFAMLERYHQTKSILGVCLGHQTICEFFGGKLYNLNDVRH
                     GQQRTLTQIEPNPIFKGLPKQLKVGLYHSWGILQNSLQNTPLVPTAVCDQNVLMAFKH
                     QHLPIFGVQFHPESFITEFGQQMLANWLEITQDF"
     gene            49007..50089
                     /gene="prfA"
                     /locus_tag="PXB27_000057"
     CDS             49007..50089
                     /gene="prfA"
                     /locus_tag="PXB27_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDL5344896.1"
                     /translation="MKDSIINKLESLSERHEELQALLGDASIISDQDKFRAYSKEYSQ
                     LEEVVGTFNRWKKLNNDIEEAQLLLDDPDMKDMAAEEIAENKAEIENLEQHLQILLLP
                     KDPNDEYNAFLEIRAGTGGDEAGIFAGDLYRMYSRYCESKRWRIEEMSANESEQGGYK
                     EIIVKISGEGVYGQLKFESGGHRVQRVPKTESQGRIHTSACTVAVMPELPESEMPEIN
                     PSDLRIDTYRSSGAGGQHVNTTDSAVRITHIPTGIVVECQDERSQHKNKAKALAVLAS
                     RIVQVEQERQAAEQADTRRNLLGSGDRSDKIRTYNYPQGRVTDHRINLTVYRLDEVMN
                     GKIDELIQPIITEYQADQLAALSEQN"
     gene            50201..50803
                     /locus_tag="PXB27_000058"
     CDS             50201..50803
                     /locus_tag="PXB27_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5344897.1"
                     /translation="MRKLGLLAVFTLVLSACSNSTKIEQTILSKQDIVGSWVCAIKYD
                     DLKVSTLDFSEFKANGEMTNFGDISDENFEPIKFTYVTGDKGKWDLKGNRLVIDYDLS
                     ERKVKRTTPKKFLEFLKQEERKASKTAKKLLKYEQGIFNILSDQKNTENSKIILDILK
                     FGHSRMAIQQNMGDKVYSGGCVTADKAEEYLDILKNRNSK"
     gene            50803..51669
                     /gene="prmC"
                     /locus_tag="PXB27_000059"
     CDS             50803..51669
                     /gene="prmC"
                     /locus_tag="PXB27_000059"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDL5344898.1"
                     /translation="MDYREWLTFAEQKLLENSGADPYLNAKFDANLLLQAVTKRSKSA
                     IFAFSETLLSESELAELAEKLARRAKGEPMAYILGYREFWSLPLKVSPATLIPRPDTE
                     RLVELALEYANKRLGNQKMLQILDLGTGTGAIALALASELGEKAQIIGVDFQNEAVQL
                     AEENRRNLGLNQVTFLQSDWFSSLANQQFDLIVSNPPYIDESDENLTVGDVRFEPLTA
                     LVADNKGLSDLQKIIENAPLHLKPQGALMLEHGWKQGNLVRDLFDLHLWEQVETAQDY
                     AGNDRVTKAILK"
     gene            51678..52544
                     /locus_tag="PXB27_000060"
     CDS             51678..52544
                     /locus_tag="PXB27_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB1 family protein"
                     /protein_id="HDL5344899.1"
                     /translation="MDDIIEELLKELEDPKVTISETELKKFLYREILRLTTLIDKNVS
                     EQQVFGQMSALVKKARYNVNGEGDEERINQLLHLVYREWGFHCYYEDYFHTENLLLNQ
                     VIRKRRGMPVSLGAVALYLSAVLDLPLYPVNFPTQLVLRAEIHQPNGATKVRFINPWN
                     GEFLTIEMLNKWLEGEMGFGVEVTPDLLRRAEPQELLERVETVFKMALTREGKYEETL
                     RLIEYRLAFSPEDPYEIRDRGMVLASMDCYQAALEDINYFIDQCPEDPSAEILKMEVK
                     GLQQKSQESVVH"
     gene            52634..53488
                     /gene="kdsA"
                     /locus_tag="PXB27_000061"
     CDS             52634..53488
                     /gene="kdsA"
                     /locus_tag="PXB27_000061"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012073169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HDL5344900.1"
                     /translation="MTDKIVKVGNIEVANHKPFTLFGGMNVLESRDMALRVCEQYVEV
                     TQKLNVPYVFKASFDKANRSSIHSYRGPGMEEGLKIFQELKQTFGVNIITDVHEIYQC
                     KPVAEVVDVIQLPAFLARQTDLVEAMARTGAVINVKKPQFLSPGQMGNIVEKIAECGN
                     DQVILCDRGTNFGYDNLVVDMLGFGIMKKVSKGCPVIFDVTHSLQCRDPFGAASGGRR
                     DQVTELARSGMAIGLAGLFLEAHPDPNSAKCDGPSALPLSKLEAFVSQMKAIDELVKS
                     FEEIDTSK"
     gene            53709..54656
                     /locus_tag="PXB27_000062"
     CDS             53709..54656
                     /locus_tag="PXB27_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HDL5344901.1"
                     /translation="MLNIVFLDRTGIPATHDIPRPNFPHTWTEYATTSADQTFERVKE
                     ADIIITSKVLLQRDLLAKLPKLKLIAITATGTNNVDLAAAKELGIAVKNVTGYSSVTV
                     PEHVIGMIYSLKHRLADYHRDLITSDRWATCGQFCYVDYPIHDVRGSTLGIFGKGNIG
                     AEVARLAQAVGMQVIFAEHQGATEIREGYTAFEEVFKQADIISLHCPLTEQTQNLINA
                     ETLALMKPTAYIINTGRGPLIDEQALLEALEQGKIAGAALDVLVKEPPEKDNPLMLAA
                     KRLPNLLITPHVAWASDSAVTTLVNKVTQNMEEFVATGM"
     gene            54754..55926
                     /locus_tag="PXB27_000063"
     CDS             54754..55926
                     /locus_tag="PXB27_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HDL5344902.1"
                     /translation="MNLTPFFIAFRYFRSKSADRFGRLVTNLASLGIVLGVMALVIVL
                     SVMNGLENMQKTNLLSTLPHAIITPQEGHFAKGEKLALPEFAKQSVVINRANVIIQSS
                     QGINAGQLIGVENATDDPLLVAENIPALLPSGEFKVVIGSRLANKLNVTVGDKLRLMI
                     TENSQYTPLGRVPVQRLFEISGVYQSNREASEFTLFANLADVGRLLRISEDQVQGVRL
                     YLQDPFQVTELSQYFDAERYQISDWREQKGEFFQAVKMEKNMMGLLISLIIVVAISNI
                     VTSLSLMVVDKQGEIAILQTQGLTKKQVMQIFVFQGAIVGVIGSIIGGLLGVLIAYNL
                     DQIILLLNPSIHLPTLISGTQIAVIVGVSMLLSLLCTLYPAYRASKIEPAQALRYE"
     gene            55943..56626
                     /gene="lolD"
                     /locus_tag="PXB27_000064"
     CDS             55943..56626
                     /gene="lolD"
                     /locus_tag="PXB27_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HDL5344903.1"
                     /translation="MTELLRCEHISKFYDEGEQKVQVLKEVSFSMNEGELVAIVGSSG
                     SGKSTLLHTLGGLDQPSTGEVWIRGQSLQQLSSDKLALLRNQNLGFVYQFHHLMADFT
                     ALENVMMPMLIGKQNKTEAANRAEKMLQAVGLAHRMTHRPSALSGGERQRVAIARALV
                     NNPALVLADEPTGNLDQKTTESIFELIQQLNQEQNIAFLLVTHDLNLANKLSRSLVMR
                     DGVLSEGSL"
     gene            56623..57888
                     /gene="lolE"
                     /locus_tag="PXB27_000065"
     CDS             56623..57888
                     /gene="lolE"
                     /locus_tag="PXB27_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HDL5344904.1"
                     /translation="MSTPFFISWRYQRGKQKNKLVSLIALFSNIGIALGVAVLIIGLS
                     AMNGFERELNNRVLSVVPHAELVSFQGNQPQPIFDSKKLEALIKKSQNVTASSPFVSF
                     TALIENGAQLKIAQVRGVDPKKQDQASQLSQFIPPEQWQAFNQQFQADEDGLILGAGI
                     AKALEVEAGDEVTLLIPQPTEDGKLAQPLRFNLNVTGILRLDGQLDHSYALIPLNKAQ
                     ELMEYQANEVSGLEISLANPFDVQQLEMPQLNGYTQPLYLNTWIDKFGYMYNDIQLIR
                     TVMYIAMVLVIGVACFNIISTLIMAVKDKQGDIAILRTLGANNGFIRRIFLWYGLISG
                     MKGALFGLILGVILSLNLTAIIKAIEAFFEVKLLSDGVYFVDFLPSELHWQDVVYVLA
                     ATIILSLFASLYPANRAAKLEPAKVLSGH"
     gene            complement(57963..59894)
                     /gene="thrS"
                     /locus_tag="PXB27_000066"
     CDS             complement(57963..59894)
                     /gene="thrS"
                     /locus_tag="PXB27_000066"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDL5344905.1"
                     /translation="MPIITLPDGSQRSFDNPVSVMEVAQSIGAGLAKATIAGRVNGER
                     RDASDIISQDSTLEIITAKDEDGLEIIRHSTAHLLGHSIKQLFPDVKMAIGPTIENGF
                     YYDIDLDRSLTQEDLDALEKRMLELAKTNYDVVKRPVSWQEARDTFEKRGEPYKMAIL
                     DENIERTATPALYHHEEYIDMCRGPHVPNMRFCHHFKLQKVAGAYWRGDSKNKMLQRI
                     YGTAWADKKQLAEYLQRLEEAAKRDHRRIGKALDLYHMQEEAPGMVFWHNDGWTIFRE
                     LETFVRTKLKEYDYQEVKGPFMMDRVLWEKTGHWQNYADLMFTTQSENREYAIKPMNC
                     PGHVQIFNQGLKSYRDLPIRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IESEVISCIKMVYDIYSTFGFTNIAVKLSTRPENRIGDDAMWDRAEKGLANALRNNGL
                     EYEIQEGEGAFYGPKIEFALRDCLGREWQCGTVQLDFALPGRLAASYVAEDNGRRTPV
                     MIHRAILGSIERFIGIITEEYAGFFPTWLAPTQAVVMNITDSQADYVQQVVKQLSDAG
                     IRVKADLRNEKVGFKVREHTLRRVPYMLVCGDKEIAEGKVSVRTRKGADLGTYLVSDL
                     VEILKNQIKARELKLLGEE"
     gene            complement(60061..61410)
                     /locus_tag="PXB27_000067"
     CDS             complement(60061..61410)
                     /locus_tag="PXB27_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5344906.1"
                     /translation="MNSTKFYSLYSGIFFLFFGYGLFLNSAGIKLAEMGITDIIIGLL
                     NAAFFIGAALSTIFAHRIVSSVGHIRSFSVFCAVFAIAALGHIMIDTLWIWGVLRIIL
                     GFCHYSLLLLVESWLSEKTNVDTRGKVLATYNIIFYLAFILGVSLLSLELSSRNIFIL
                     STILVVMSMIPIALTKMVQPDIPARERISIPKLFAISPLALATSFISGMLVNGFFTMA
                     SVFLLKFSFSLSEISLYLMISMIGGFISQMPMAILSNRIGRRNAILACSVIATLTALA
                     GLFAIFQENLTAWGQYIIAFFFGCSLFSLYALSIARANDELPNNMNTVEVSRGLLFCY
                     GVGALVAPPLIGFAMSLSAEYGFYALFGLFSLSLFICAFRTKSIPKAERAEMQYATPI
                     ATASPLEEIEVVEDALIPFDEEAVQEYQAQLEILEQDPQTENENIELDEGAAENSKV"
     gene            complement(61685..62176)
                     /locus_tag="PXB27_000068"
     CDS             complement(61685..62176)
                     /locus_tag="PXB27_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDL5344907.1"
                     /translation="MFTTQLIQTKRWLEANSITTFWLKTFIGATLLAIFAQISVPMYP
                     VPITGQTLALTVVGFAMGRKAATAAVLLYLFEGAIGLPVFAGGASGFHHFFGPTGGYL
                     LGYIPTAYFLGYYSDKGVLNSFWKSLFVALIASVITFAFGLAQLSLYQKAQYYKRAYI
                     HLF"
     gene            62257..63024
                     /locus_tag="PXB27_000069"
     CDS             62257..63024
                     /locus_tag="PXB27_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--protein ligase"
                     /protein_id="HDL5344908.1"
                     /translation="MVKPVYIYCDEGSSEIGVQSLLLAVKQHLQRPAYLINAKQIIQS
                     KFSDGCLLIMPGGADLPYCKKLNGEGNRRIRQFIEQGGAYLGICAGAYYGCAELNFVG
                     RDYSVNGNRELALFNGIAGIAKGSLPQFTNGQLYDKNIGSKAIVEVTLAEGGKMPFYY
                     HGGCCFEPNSTTVIYNPLAYYPDGSVAMIEGELGRGYYLLSGVHFELQAVVYWQKIVN
                     CCENLDIKTQEEAICSNFNVNYGSQIWQHLKQRLNQA"
     gene            complement(63015..63809)
                     /locus_tag="PXB27_000070"
     CDS             complement(63015..63809)
                     /locus_tag="PXB27_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDL5344909.1"
                     /translation="MRFFDTHTHLDYLAESLNLSIPELVENAKQHQVECILIVAVFAK
                     NFAKVTACAKQKPENLVYGLGLHPLYIQQHQQQDLILLENLLSQQDPQCTAIAEIGLE
                     KAVSEVSTPELWKKQCEFLEAQLSLAKKFNLPVNLHSRKSHDQLFVFLKKAQLTKTGV
                     VHGFAGSYEQAKRFVDLGYKIGVGGTITYERANKTREAIRKLPLESLLLETDSPDMPV
                     FGFQGQPNRPERLLVTFNSLCELRTESPNLIAETIWQNSERLFFYA"
     gene            complement(63870..65456)
                     /locus_tag="PXB27_000071"
     CDS             complement(63870..65456)
                     /locus_tag="PXB27_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HDL5344910.1"
                     /translation="MLWFFFCVAVLVLGYFTYGKIIEKIFVINPKRATPAYAMNDGVD
                     YMPMSKTKIWLIQLLNIAGTGPIFGPILGALYGPVAMLWIVIGCIFAGAVHDYFCGML
                     SIRNGGATMPNLAGKFLGRPVKLFINVMALILLLLVGVVFVASPAQLLSTITMDIAGA
                     TQSAMELGDVEAIKESVASGGVTVWGMDKATVISVWTLIIFAYYILATLLPVDKIIGR
                     IYPFFGALLLFMSVGMVYGLVSAHLSATDPIDFFRTVNADGQGLTWEKFTQNFQVKGD
                     VPIWPLLFLTISCGALSGFHATQTPLMARCAQNEKEGRFIFYGAMITEGVIALVWCAV
                     GLSFYENPQALQDAISAGSPSKVVYDSSLHFLGFIGGIFAVLGVVVLPITSGDTAFRA
                     ARLQLAEIFGLEQRTLVKRLYIAVPLFVLGFIVSKVDFSVLWRYFTWANQMTAMVMLW
                     TAAGYLYRYKKFHWVCSIPAWFITTVCATYLANNKIGFGLDYELSVYIGFAITVVCVI
                     CFFAFLKPSEARDEDAYVTN"
     gene            complement(65642..67495)
                     /gene="dxs"
                     /locus_tag="PXB27_000072"
     CDS             complement(65642..67495)
                     /gene="dxs"
                     /locus_tag="PXB27_000072"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDL5344911.1"
                     /translation="MEKKYPLLSEINSPEDLRLLSKDQLQTVVDELRAYLLESVSQTS
                     GHLASGLGVVELTVALHYVYQTPFDQLIWDVGHQAYPHKILTGRRDQMHTIRQKDGIH
                     PFPWREESPYDVLSVGHSSTSISAGLGIAVAAEKENAGRKTVCVIGDGAITAGMAFEA
                     LNHAGALHTDMLVILNDNEMSISENVGALNNHLARIFSGSLYTSVRDGSKKILDKVPT
                     IKNFMKKSEEHMKGVIFSPESTLFEELGFNYIGPIDGHNIDELVKTLSNMRELKGPQF
                     LHIRTKKGKGYTPAENDPIGYHGVPKFDPTAAELPKSKAAPTYSNIFGDWLCEMAERD
                     PKIIGITPAMREGSGMVEFSKRFPEQYFDVAIAEQHAVTFGAGLAIAGYKPVVAIYSS
                     FLQRAYDQLIHDVAIQNLPVIFAIDRAGIVGADGQTHQGAFDLSFMRCIPNMTIMAPS
                     DENEMRKMLYTAYTMNTPSAIRYPRGNAKGVKLEPMQRLEVGKGNIIREGKKVAILNF
                     GALLDEAKIVAEKHNYTLVDMRFIKPLDEALLQKVADSHELLVTLEENAIQGGAGSFV
                     NEYLQNIGKIKPLVMLGIPDFFVPQSTQAEAYADLGLDAQGIEAKIQKFDI"
     gene            complement(67540..68352)
                     /gene="suhB"
                     /locus_tag="PXB27_000073"
     CDS             complement(67540..68352)
                     /gene="suhB"
                     /locus_tag="PXB27_000073"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012621556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HDL5344912.1"
                     /translation="MNPMLNIAIRAARKAGNIIAKGYEQSPQDTQVLQKNTNDYVTEI
                     DKAAEAAIIDVIRKSYPEHSIVTEESGVLNGSNENVQWVIDPLDGTTNFVKRLPHFAV
                     SIAVRENGRTTVGVVYDPIRNELFTAVRGEGAKLNEFRLRIDAERRDLKGTVIATGFP
                     FKVIRHRDAHLNMLEGLMNNGVADFRRTGSAALDLAYVAAGRVDGFFEIGLKPWDFAA
                     GELIAREAGAIVTNFVGGTDYLKTGNLVVGNGRVVKEILNSIQPTLTEELKA"
     gene            68533..69567
                     /locus_tag="PXB27_000074"
     CDS             68533..69567
                     /locus_tag="PXB27_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA methyltransferase"
                     /protein_id="HDL5344913.1"
                     /translation="MQFKENNQPRFKHREERRSDECSPRFSDKGKMRGNEGKSDRPHK
                     LHFKRDEAYSRSEQIYPAEGKKASGEGSVQIWVKGGNSSVKEKKNGPLSPRAPEKIKK
                     NRLEEMKVYGENACISLFQQRPESIVRLWATVEGAKKLGDLMSYLAQHKKAYHVIDRE
                     EMERVTGTEHHGDMCLLVKKTASFVLEGYLQVPKKQDCLVLLDGVNNAQNVGGIIRTC
                     AFYGVKGIITENVDSLNSSAAARVAEGGLEFVRALETKNKHIALVQLRQAGYQIVHLT
                     RHKQAPSLTKIKLAEKVVFVLSEIPASDVAYAEDTTIQLSFNNPLNSGLNVAVNAGIL
                     LNQWYQTHIL"
     gene            69577..70461
                     /locus_tag="PXB27_000075"
     CDS             69577..70461
                     /locus_tag="PXB27_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HDL5344914.1"
                     /translation="MNQQKPFEVIAIVGTPRHDSAFETHLAVYNWLKDRDYQILVETE
                     VATQLQLKESYSLDQIGQRAELVIVIGGDGNMLGIARALAKYRVPLIGINRGNLGFLT
                     DIAPQTAFEQLYSCLVKNEYVIEERFLLEAFIERNGKIIAANNALNEIVVHPTQVARI
                     IEFEVYIDGKFAFSQRADGLIVATPTGSTAYSLSAGGPILTPNMNAMALVPMHPHTLS
                     SRPLVVDGDSQISLRFAKYNTPDLEVICDGQVNMHFTPEDRVLVRKSPDKLQLLHLKD
                     YSYFTVLGSKLGWASKLF"
     gene            70600..71577
                     /locus_tag="PXB27_000076"
     CDS             70600..71577
                     /locus_tag="PXB27_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1852 domain-containing protein"
                     /protein_id="HDL5344915.1"
                     /translation="MTTNFKCQVHTIEFNEDYQPEQSTRLTTNFANLARGENRKQNLQ
                     NAIAMINNRFNDLANWDNPNADRYSVKLDIVSLDIDVEGKGEFFPAIEWLKTYVIDYK
                     TGKTIDGIIGNNFSSFVRDYDFSVLLLDHNKDKPQFSVPEGYGELHGKLFKHLLASPE
                     YTAKYKKPPVICLSVATTRTYTRTTNVHPILGVEYLQDSYSLTDAYFAKMGLKVRYFM
                     PPKSVAPYAFYFDENADLLNDYTNLELISTIATMEAFQKIYRPEIYNANSVAGQVYKG
                     SLTYQDYSVTKVEYDREERTQLAIKQGKYTEEHFIKRYQAILEAWATEE"
     gene            71665..72696
                     /locus_tag="PXB27_000077"
     CDS             71665..72696
                     /locus_tag="PXB27_000077"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDL5344916.1"
                     /translation="MSNILLPTSTAGSLPKPSWLAEPEKLWSPWKFEGEELLQAKRDA
                     LLVSLAEQIAAGIDIVSDGEQTRQHFVTTFIEHLEGVDFSKREIVRIRNRYDASVPSV
                     VGAVSRPKSVFVDDAKFLRAHTDKPIKWALPGPMTMIDTLYDGHYKSREKLAWEFAKI
                     LNEEAKELEAAGVDIIQFDEPAFNVFFDELNDWGVAALERAAEGLKCETAVHICYGYG
                     IKANTDWKQTLGNEWRQYEESFPKLQQSKIDIISLECQNSRVPMDLIELVRGKKVMLG
                     AIDVATNIIETPEQVADTLRKALQFVDADKLYPSTNCGMAPLSRQVAQGKLQALSQGA
                     AILRAEFAK"
     gene            72784..73005
                     /locus_tag="PXB27_000078"
     CDS             72784..73005
                     /locus_tag="PXB27_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5344917.1"
                     /translation="MLNLFKKPIKKSSFEAWAKLMDDVAKVAILAIPVILYGEYSLLM
                     KFTHSITLFAVVYFLLATARLFRETAEEM"
     gene            73281..74543
                     /locus_tag="PXB27_000079"
     CDS             73281..74543
                     /locus_tag="PXB27_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDL5344918.1"
                     /translation="MLKRDMNIADYDAVLWQAIQDEDRRQEEHIELIASENYASPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKALFGADYANVQPHSGSQAN
                     AAVYMALLNPGDTILGMSLAHGGHLTHGASVSFSGKIYKAEQYGITDEGLIDYEALRK
                     QAQEVKPKMIVAGFSAYSQVMDWAKMREIADEVGAYLFVDMAHVAGLIAAGVYPNPLP
                     HAHVVTTTTHKTLAGPRGGLILSASGDEEMYKKLQSAVFPAGQGGPLMHVIAAKAVCF
                     KEALEPEFKAYQQQVVKNAKAMVEVFKQRGFDVVSNGTENHLFLVSFVKQGLTGKAAD
                     AALGAANITVNKNSVPNDPQKPFVTSGIRVGTPSVTRRGFKEAEVTALAGWMCDVLDS
                     IGKDNHEQVIAETKAKVLDICKRLPVYA"
     gene            74701..75537
                     /locus_tag="PXB27_000080"
     CDS             74701..75537
                     /locus_tag="PXB27_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HDL5344919.1"
                     /translation="MLPRISSFLASLFMAFFLLGCSVSTQSLNEQNVDSIGQIIDLNT
                     NQTISFTELLSRLSSQDYVLVGEEHTQLIHHQIELYLFNQLTKKAKLHLIALEMLNVD
                     QQPAIDRIQFNRPVNLSATDLREAIQWQKWDWKMYQDLVVESLGSNSRVIATNLTDKE
                     VEILLNGAEPLKGSVSTSSEVKQKIAQLLLSMNHGMPYPMENMVAVQQFRDRRMAEKL
                     VKNALSTSLLIAGNHHVRKDLGVPLHIAEYDRTKKVAVLMLKTEREEITSSQADYLWV
                     TQ"
     gene            75591..77748
                     /locus_tag="PXB27_000081"
                     /pseudo
     CDS             75591..77748
                     /locus_tag="PXB27_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484758.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            77855..78493
                     /gene="purN"
                     /locus_tag="PXB27_000082"
     CDS             77855..78493
                     /gene="purN"
                     /locus_tag="PXB27_000082"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDL5344920.1"
                     /translation="MKKFVVLISGNGSNLQAMIDAQKSADTSGQICGVICNKADAYGL
                     IRAKQAGIPTFVFSRKDYQSNVEMDLAIAEQIEQLGAELIVLAGYMKILTPEFTQHFA
                     GKILNIHPSLLPKYPGLNTYQRAIEAGESEHGTTIHFVNEEVDAGAVVLQAKVPIYPE
                     DEIEDVMARVVEQEHRYYPLVIEWFCSGRLVSQHGKAYLDGKLLPACGYAED"
     gene            complement(78514..79320)
                     /locus_tag="PXB27_000083"
     CDS             complement(78514..79320)
                     /locus_tag="PXB27_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5344921.1"
                     /translation="MSNLLTCQNLSIVHQQKKLLEIQEIAFEQGKFTAIIGPNGAGKT
                     TLLKALSGNNAQTNSAVFYQHQRLTDLNGYALAQKRAMLSQQSQIAFPLKVEELVQLG
                     REPYRQSEFAKFNEKITAWAMETMSLTALKERSSIQLSGGEQHRAHIARVLAQLVPEP
                     NADLSGSWLLLDEPTNHLDIHHQYQLFSLLQKLKQQGLTIIAILHDPALAINQADHIL
                     MLKNGEKFAEMSAKAIAESCLLDELYEMKMGCRYCPHSGQYYLCPALAES"
     gene            complement(79320..80306)
                     /locus_tag="PXB27_000084"
     CDS             complement(79320..80306)
                     /locus_tag="PXB27_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL5344922.1"
                     /translation="MKLHLTLIFTLIISLVICTHLGAMSLPLSAWHCNNAECESAAYI
                     LWQIRLPRLLGALFIGAALAISGATMQGLFRNPLVDPGIIGVSSGAALAAALYIVVIQ
                     PLLPESISYYALPIAAFLGGWLVTFMLYLLSQRNGQLHIAIMLLIGIALAAFSGALTG
                     ILIYVADDNQLRSLTFWSMGSLAGMNWQMVAMMAAVVFICSPLIWREHRALNALTLGE
                     EDAQYIGFDIAKVKRRLVFYVALLTGTSVACVGAIGFVGLVIPHLMRLLGGSNHRHLL
                     LNSLVCGAILLMIADTLARTLAAPAEIPIGIFTALFGAPFLAVMVWKSGKHI"
     gene            complement(80317..81183)
                     /locus_tag="PXB27_000085"
     CDS             complement(80317..81183)
                     /locus_tag="PXB27_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5344923.1"
                     /translation="MEYKMRTLLFSFIALSMCLNTTKAAEKIVSTAGYASEIVAALGK
                     ADKLVGVDTTSVKPQTIMEKKPKIGYRRQLSAEGILSLTPDLIILAPDAGPNAVIEQI
                     KASGIPLLHLGEKPSLAGIREDIQQIAKAIGAESEAKQLTETILADEKKLADIRSQHS
                     TDKEVLVLIDTASQGIFGLGKKSVGDHFLTLLNLNNSFNAEGNKPLANEALAASNADV
                     ILLASRNQAKESATISKLAESSPQYAQLTNTQAGKKGCVFAINILDALGFGPYTAQYA
                     HQILTTIQPCLK"
     gene            81290..>83440
                     /locus_tag="PXB27_000086"
     CDS             81290..>83440
                     /locus_tag="PXB27_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HDL5344924.1"
                     /translation="MQIKQTVLAIAITPFISQLSLAQETKISSIELDTIEVNEQQENL
                     LKQQAVMSNEDLVRYSTDMGVPQTGRFGPQGFNIRGVEGNQVAVTVDGISQAASNNYA
                     TFSRYSYYNKSRPEIDTELMSDVTIEKGSSTKVGNGALGGSVNYKTKTVSDILMKGRQ
                     FGGMLKYTYNGQNKENIETAGIAADGQYADALVMYSHRQGHETQTTSKGPDIMGSGRG
                     LPNPYKNNQYSYLAKLGLKWNDENRLELKTSKQRSKNAGQELSYSTVTLRDFLDVQEI
                     ETYGVTYLYTPKNSILENIKVAVTKQDTEISGINDQITIARGTKTDNTKRAFNTKNVE
                     GSLGVTFKPVDLGNTEHKLSTELGYGVSDFSFDMQTYTAGITPTDQNMQSPAKTKSFH
                     ILMGDSILLTQNLSAHLNARYEQSKLHSDDNSLSDKTFNNWAGDIGLNYQLSPVWQIG
                     YKLSRGFRTPTASEMFFNREVQGRGASQTFLANTNLKPETSLGNEISLVGNGSLGSLS
                     VAAYHTHYRNLIDLATIGNNIYQSQNVNRAKIKGVDINGTLDLNTAWNAIPQGFELNG
                     GFGYARGKRNDGTDLLTVQPLKALFGIGYRAVNNDWAIHLNGTYTQGKKAKYAQQYAI
                     LANTGYLSYDKSLTTYPYLSNSATVFDLVAHKKFGERAIVKVGVYNLFNKKYWTWDSL
                     RTIGITSPGVANSITGEGLNRYLAPERYVMASVEF"
BASE COUNT        24958 a        16346 c        17967 g        24169 t
ORIGIN      
        1 tattaattaa gattagtcgg cattattgaa aaaagaatta atctgactgc ttgccgattt
       61 tccattagca aaaaaatgta atttgatgta aactactttc ccgtcctgat agtttctttt
      121 ttacgaagta aactgatatt ctgaatttcc aacctaatca atcagcaaag gcaatcacaa
      181 tgacaacgaa ttatattttc gtcacaggcg gtgttgtatc ttctttaggg aagggtattg
      241 cggcagcctc tttagcatct attctggaag ctcgtggttt aaatgtaacc attatgaagc
      301 ttgatcctta tatcaatgtc gatccgggta caatgagtcc aactcagcac ggggaggtat
      361 ttgtgaccga agacggtgcg gaaaccgatt tggatttagg tcactacgag cgttttatcc
      421 gttcaaaaat gagcaaagcc aacaacttta ccagcggtaa aatttactct gaagtgttgc
      481 gtaaagaacg ccgtggcgac tatttaggtg caaccattca ggttattccg catattacca
      541 acgagattaa agcccgtgtg ttagaaggcg gtaaagggcg tgatgtcgcc attgtagaag
      601 taggtggcac ggtgggggat attgaatcac tgccattctt agaagcactc cgccaattag
      661 cggtggatat cggtcgtgaa aaaaccttgt ttatgcattt aaccttagtg ccttatattc
      721 caaccgcagg ggaagtgaaa actaagccga ctcagcattc agtaaaagag ttgctttcaa
      781 tcggtattca gccggatgtg ttaatttgcc gttcagatcg tgctattccg gaaaatgaga
      841 agaaaaaaat cgcccttttc tgtaatgtgc cggagcgtgc ggttatttcg cttaaagatg
      901 tagattcgat ttatcgcatt ccggaattat tgaaatcgca agggttagat tcctttgtgt
      961 gcgatcgttt ccgtttagat tgcaaagaag ccgatctttc agagtgggaa caagtgctat
     1021 actgccaagc aaacccaacc ggcgaagtga ctatcggtat ggtcggaaaa tatgtggaat
     1081 tacctgatgc gtataaatcg gtgaatgagg ctttgaaaca cgcaggttta acgcatcgtt
     1141 taacagtgaa tattaaatac attgattcac aagatattga aaccaaaggc acagagttat
     1201 tggcaggctt agacggcatt cttgtgccgg gcggctttgg ctatcgtggc gtggaaggca
     1261 aaattcgaac cgcacaatat gcccgtgaaa acaagattcc ttacttaggt atctgtttag
     1321 gtatgcagat cgccctaatt gaatatgccc gtaatgtggc aggtttaacc gaggcaaatt
     1381 cttctgaatt tgataaagat tgctcacagc cggtgattgg cttaattacc gaatggcaag
     1441 atgcggaagg gaatgtggaa acccgcagcg atgagtcaga tttaggtggt acaatgcgtt
     1501 taggctcaca acaatgccat ttaattgaag gctcaaaagc tcgtgagtta tatggtgcgg
     1561 aaacgattgt tgaacgtcat cgccaccgtt acgaagtaaa caataagctg ttaccacaaa
     1621 ttgaagcggc aggcttaaaa gtgagcggtt tatcggcaga tcgtaaatta gtggaaatta
     1681 ttgaagtgcc aaatcaccct tggtttatag cagcacaatt ccatccggaa tttacctcaa
     1741 ctccgcgtga tggtcatcca ttatttgcag gttttgtgaa agctgcgaaa gatcaccaag
     1801 agagtcgtaa ggcttaatca aacttaacaa gcggtcgaat ttactcattt ttttgtacaa
     1861 acgcttgcgt ttgaacatcg gctttgctga tggcacgaag tgcgagcgaa agcgagtaaa
     1921 tttgaccgct tgctcattaa ttataagaaa gggataaatc aatgatttat cccttttagt
     1981 atttttaata gtaagaatgt tcaccgtgtt ggtgttcggt aatatctttc acaccaacta
     2041 attcatcagg gaacattgcc agaagctgtt tctcaattcc ctcttttaag gtcacatcca
     2101 ccattgagca accgttacaa ccaccgccga attgtagcac ggcatattta tcttcggtga
     2161 tttcaattaa ggtcacttta ccgccgtggc tggcaagttg tggattgact tgggtttgaa
     2221 tcacataatc taaacgctcg ataaacggtg catcatcagc cactttacgc attttggcgt
     2281 taggggcttt gagggtaagt tgtgaaccca ttgggtcggt tacataatca atttccgctt
     2341 cgtctaaaaa cggtaagcta atatcatcga caaatgcaga gaaacggtta aatttaaatt
     2401 cggtatccgt ctcttcaact gcattgggtg ggcagtaaga gacaccgcat tctgcccccg
     2461 gtgtgccggg attgaccaca aaaatacgga tattagttcc ctcttcttgc tgttctaata
     2521 gtctggcaaa atgggcttgt gctgcatctg atattgaaat atgaatggtt tgttcttcca
     2581 tttaaattat cctgtttttt aatattttac aaaatttatc aactatttta cgcctgaatt
     2641 tcaaaattgc ctagctaaaa aaatttttgc aaattgttaa taaaatttaa ccgcttgaag
     2701 ggaaattgga ttaactaatt atggttaatt tatgttaaga gggaattgaa atcttgattt
     2761 atttcaaatt ttaccgcggg aatatgcaaa aaataggggg ttttcctagc cagaagggga
     2821 gctttttagg taaaataaga agattatttt tagttaacct tttgttaaga aaagataaag
     2881 gttgatcaca gatttgagaa cttaatgtgg cttttaaaaa taggtggggg accattgttt
     2941 atttttaagt aaagctgcgg ctcaatagaa gcgattgcgt tagcgcaatc atatttttta
     3001 acccaaaaga aaagtagtgc aaacaatatg attacaatca aaaaaggctt agatcttccg
     3061 attgcgggaa aacctgagca agtaatccat aacggtaata ccgttactga agttgctatg
     3121 cttggtgaag aatatgtggg tatgcgtcct tcaatgaaag tgcgtgaagg cgatgtagta
     3181 aagaaaggtc aggttctttt tgaagataaa aagaatccgg gcgtggtatt tactgcccct
     3241 gcaagtggta ccgtaacggc aattaaccgt ggcgagaagc gtgttcttca atcggtagta
     3301 attaaagttg agggtgatga gcaaattacc tttgctcgtt atgaagccag ccaattagct
     3361 tcattaactt cagagcaagt aaaacaaaac cttgtagagt caggcttatg gacggctttc
     3421 cgtacacgtc cgtttagcaa agtgcctgca ttagatgcag aaccttcctc tatttttgtc
     3481 aatgcgatgg ataccaatcc acttgcagca aatccggaag tgattattgc agaacaccaa
     3541 caagctttct ttgatggctt aactgtatta agtcgtttat ttgaaagtaa aaaagtgatt
     3601 catctctgtg gcagtgcaga ctcaaatatt ttaaatgcgg cacaaagccg tcagatcaat
     3661 aatgtggaaa ctaaccgctt taaaggtccg caccctgcag gtctttctgg tacgcatatt
     3721 cactttattg acccggtagg tgcaactaaa caagtttggt atttaaatta ccaagatgta
     3781 attgctatcg gtaaattatt tacgaccggt gagctattta ctgaccgtgt agtcgcatta
     3841 gccgggccac aagtgaaaaa tccacgtttg gtgcgtactc gtttaggggc gaatctttca
     3901 caattaacag ccaacgaatt aaatgcgggt gaaaaccgtg tcatttcagg ttctgtgtta
     3961 agtggtgcta tcgcaacggg agtacacgac tatttaggtc gttacgcatt acaagtgtct
     4021 gctttattag aagggcgtga gaaagcgttc ttcggtatga ttgctcctta ctctgataag
     4081 tattcaatta cgcgtactac tttagggcat tttgcgaaga aattatttaa cttcacaaca
     4141 gcagttcacg gtagtgagcg tgcaatggta ccaacagggg cttatgagcg tgtaatgccg
     4201 ttggatatca ttccaacact cttattgcgt gatttagcag ctggagatac agactccgct
     4261 caagctttag gctgcttaga gttagacgaa gaagatttag cgttatgtac ttttgtgtgt
     4321 tccggtaaaa acaactgggg accattatta cgtcaagcgt tagataagat cgagaaggac
     4381 ggttaaaaat gggtttgaaa aatctttttg aaaaaatgga acccgctttc cataaaggtg
     4441 gaaagtatga gaaatggtac acgctatttg aagctgccta tacgattttc tatacaccgg
     4501 gtacggtcac tcgtaaagat gctcacgtgc gtgatgcgat tgactctaag cgtatgatgt
     4561 taattgtatg gttagcgtta ttccctgcga tgttctacgg aatgtacaat gttggtgcac
     4621 agggtattct agcggcactc aaaatgggaa cattaacgga tttagttgct aacaactggc
     4681 actacagctt agccgatagt ttaggctctg tagctgatgc aggctgggga actaaaatgt
     4741 tgctgggggc aacctatttc ctaccaatct acttaacagt atttgcagtg ggcggtttct
     4801 gggaagtggt gtttgcaatg gtgcgtaagc acgagatcaa cgaaggtttc tttgtaacct
     4861 ctatcttact agcattaatt gttcctccaa cattaccgtt atggcaagca gcccttgcaa
     4921 ctacctttgg tgttgtggtc gcaaaagaag tgttcggtgg cgtgggtaaa aactttatga
     4981 accctgcgtt agccggtcgt gctttcttgt tctttgctta cccgggtcaa atttcaggtg
     5041 atgcagtatg ggttgccgca gacggcttct caggtgcaac tgcactttct caatgggcag
     5101 ttggtggtca aggtgcatta cagcacgttg caaccggtga aaccattact tggatggatg
     5161 cgttcttagg taatattccg ggttctatcg gcgagacctc taccttaatg ttgattattg
     5221 gtgctgcaat cattgtattt gcccgaattg cttcttggcg aattattgcc ggtgtaatga
     5281 ttggtatggc ggcaactgcg acattattta atgtaatcgg ttctgatacc aacccattat
     5341 tctctatgcc ttggcattgg catttagtat taggcggttt tgcgatcggt atgttcttta
     5401 tggcaaccga cccggtatca gctgcattta ccaacaaagg taaatgggca tacggtatct
     5461 taatcggctt tatgtgtgta ttaatccgtg tggtaaaccc agcataccct gaaggtatga
     5521 tgttagcgat cttatttgct aacttatttg ctccaatctt tgactattta gtagttcaag
     5581 ggaatatcaa acgcagatta gcgagggtag caaacaatgg ctaaatttaa taaagatagc
     5641 gttggcggaa cgctaaccgt tgtagtttta ttaagtttaa tttgttcttt gattgtggca
     5701 ggtgcggcag taatgcttaa accaacacaa gatattcaga aacaacttga taaacagaaa
     5761 aacatcttac aagcggccgg tttattgcaa aaagatacca atgtgcaaga aacctatacc
     5821 aaatttattg agccgaaaat tgtcgattta gcaacaggtg attacgttga gggtgtaacc
     5881 ggttttgatg ctcgtgcagc agctaaagat cctgctcaaa atgtagttat cgcacctgaa
     5941 gatgataagg cgaatatcaa aacgcgtgct aaatatgctg aagtttattt agtaaaagat
     6001 gaatctggca aaacgactca agtagtatta ccaatgcacg gtaacggctt atggtcaatt
     6061 atgtacggtt tcgttgcggt acaacctgat gcaaataccg ttaacggtat tacttactat
     6121 gaacaaggtg aaacggcagg cttaggtggc gaaattgcaa acccgaactg gcagaaaaac
     6181 tttgtaggta aaaaactgta taacgagcaa aaccaagttg cgttaagagt gggtaaaggg
     6241 gcatctgcag ataaagatca cggtgttgat ggtttatctg gtgcaacatt aacctcgaac
     6301 ggtgttgatg gttcattcaa atactggttt ggtcaaaatg gttttggtcc atatttagca
     6361 aaatttaaag cagcggcagg agctaactaa tggcagacaa taacaatctt aaaaaattat
     6421 tgttatcgcc aattgtcgat aacaacccga tcgcattaca gatcttaggt atttgttctg
     6481 cgttagcggt aacgactcaa ttacaaacag cagtagtaat ggcgattgcg gtaagtttag
     6541 ttactgcatt ctcaagtatg ttcatttcaa tgattcgtaa ctatattcca aatagtatcc
     6601 gtatcattgt gcaaatggcg attattgcat cattagtaat tttggttgac caaatcttac
     6661 gcgcttatgc ttacgatctg tcaaaacaat tatcggtatt cgtaggcctt attattacca
     6721 actgtatcgt aatggggcgt gcagaagcat tcgcgatgaa atcaggcccg gttgaaagtt
     6781 ttgttgatgg tatcggtaac ggtttaggct acggtgcaat gttaattatc gttgcctttt
     6841 tacgtgaact tatcggttca ggtaaaattt tcggtatcac cattcttgaa actgttcaaa
     6901 atggcggttg gtatcaagca aacggtttat tcttattagc accaagtgcg ttctttatta
     6961 tcggatttgt gatctgggcg attagaacgt ggaaaccgga acaagtggag aaataagatg
     7021 gaacatcttc tgagtatctt tgttaagtcc atatttattg aaaatatggc actttctttc
     7081 ttccttggta tgtgtacatt ccttgcggtg tctaagaaag tttcaactgc ttttggttta
     7141 ggtattgcgg taattgtagt acttggcatt gcagttccag ctaaccaatt agtttatacc
     7201 cacgtattaa aagagggtgc attagtagaa ggtgtggatt taagcttctt aaactttatc
     7261 actttcatcg gggttattgc ggcacttgtt caaattctgg aaatgatttt agacaagttt
     7321 ttcccggcac tttatagtgc attaggtatc ttcttaccac ttatcaccgt aaactgtgcg
     7381 atttttggtg gtgtatcgtt tatggtacaa cgtgagtata actttgtaga atctgtggtt
     7441 tatggcgtag gtgcaggtac aggctggatg ttagccatcg ttgcccttgc aggtttaacc
     7501 gagaaaatga aatattccga cgtgccggca ggcttacgtg gtttaggtat tacctttatt
     7561 acggtaggct taatggcgtt aggctttatg tcattctcag gcattcaatt ataaggagca
     7621 tatcgtggat agtaatttta ttttcggtat tatcgcattt acggctctgg tattagtgct
     7681 tgcggtgatc attcttttcg ctaaatcaaa attagttgat tcaggtgata ttaccatctc
     7741 aatcaacaac gatccggaaa aaggcattac gctaccggct ggtggaaaat tactcggtgc
     7801 tttagcaagt aaagggattt tcgtttcgtc agcttgtggg ggcggtggct cttgcggtca
     7861 atgtaaagta caagtgaaat caggcggtgg tgaaattctt ccaactgagt tatcgcacat
     7921 ttctaagaaa gaagcgaaag aaggctggcg tttagcttgt caggtaaacg tgaaatcttc
     7981 aatggacgtt gaacttccgg aagagatctt tggtgtgaaa aaatgggaat gtaccgttat
     8041 ctcaaacgat aacaaagcaa ccttcatcaa agagcttaaa cttcaaattc ctgaaggcga
     8101 agaagtaccg ttccgtgcgg gtggttatat ccaaattgaa gctgaacctc ataccgttca
     8161 ttataaagac ttcgatattc caaaagagta ccacgaagac tgggataaat ttgacttatg
     8221 gcgttatact tcaaaagtgg acgagcatat tattcgtgca tactcaatgg cttcatatcc
     8281 ggaagagaaa ggcattatta tgcttaacgt gcgtattgct acacctccac cacgcaatcc
     8341 ggacgtaccg ccgggtcaaa tgtcttccta catttggtca ttaaaaccgg gtgataaagt
     8401 aacaatttca ggtccattcg gtgagttctt cgctaaagat accgatgcgg aaatggtctt
     8461 tatcggtggt ggtgcaggta tggcaccaat gcgttcacat atcttcgacc aattaaaacg
     8521 tttaaaatct aaacgtaaaa tgtcattctg gtatggtgct cgttctgagc gtgaaatgtt
     8581 ctatgtggaa gattttgatg gtcttcaagc agaaaacgat aacttcaaat ggtatgtggc
     8641 actttctgat ccattaccgg gcgacagaga agattacttc cgtggcttta ttcataacgt
     8701 gctttatgag aactacctca aaaaccacga agcacctgaa gactgcgaat actatatgtg
     8761 tggtccaccg gtgatgaatg ctgcggtaat cggtatgctg aagagtttag gtgtagaaga
     8821 cgaaaacatc ttattagatg actttggtgg ttgataaacc ttaacttctg actacaagcg
     8881 gtcaaatttg caaaattgac cgcttgattt aatttatatt attaagtttt ggtaatttaa
     8941 taatgtgaat tttaagggga agcaaatgga acaactggat attacggtac ttgaaaatgc
     9001 cttcaattcc cttgagaaaa cgattgtcag ccttgagaat caagcgtggt ttaaccggca
     9061 agaagcaatt attcaagaca ctttgattgc aggagcaatc caaaaatttg aatttgttta
     9121 cgaattgtcg ataaaaatgc tcaagcgtca gctcaaaaac attgagtcaa atgacactga
     9181 tattgatagc accgagttcc gtgatatttt gcgaatggct gctcaatatg ggctgattga
     9241 atcaccggaa gtttggctgg attatcgcaa aatgcgaaat attacttcgc atacttatga
     9301 tcaaaataaa gcacagcagg tttataacgg aatttcaaac tttctggaaa gtacgtattt
     9361 tttagttaaa cagctccaaa aacgcaatag ctatgattga tttaactgag tcacaacgcc
     9421 aacaggtgtt ggatattttc aaaaaatact tgcctgatac acccgtttgg gtttttggct
     9481 ctcgcattaa aggcacagcc aaagcctatt cggatcttga tttagcatta atcaccaaac
     9541 aaccgctcac aattcggcaa caaacagagt tagagcttgc gtttacggat tctgacctac
     9601 ctttcacagt agatctgatt gactgggcaa gttgcaacga atcgtttaaa caaattattt
     9661 tacagcgata tgaagtgctt atttaatcgc taaatatgct ataatttccc caatttttaa
     9721 cttttgtgac accaatgaaa aaattttttc ttcttttaat tgcttttttt actttaagtg
     9781 cttgtgaaaa agcccctgag caaattacgc tgtcaggcaa aaccatggga acgacttaca
     9841 ccgtaaaata tattgataat ggtgaagtgg agaatttgat ttcgcctgaa aaaatgaaaa
     9901 atgagctgga tggcttattg attgaggtga ataatcagat gtccacctac caacaagatt
     9961 cggaaattag ccgttttaat caagcaaagc aggcaaatca agcggtcgaa atttcgcagg
    10021 attttgcaaa agtggtacaa gaggcaatcc gtttaaataa agtaaccgaa ggggctttag
    10081 atgtaaccgt tggtccattg gtgaacttgt ggggcttcgg gccggataaa cgcttaaata
    10141 aagtgccgtc tgatgaacaa attgcagagc gttctgctta tgtcggcatt gataaaattt
    10201 tgcttgaaat gagtagtaaa cccaagctca ccaagtcagc agctaatctt tatctagatt
    10261 tatcgtctat tgcaaagggt ttcggtgtag ataaattagc cgagcatctt gaaactatag
    10321 gtgtgagcaa ctatttagtt gaaattggtg gcgaattacg aggcaaagga aaaaatttac
    10381 aaggcttaga ttggcggatt gcaatcgaga agccggtaat gacacaaggt caagccgcac
    10441 aaattaccgt gccgttgcac aatttggcga tggcaacttc gggcaattat cgtaattatt
    10501 ttgaagatga aaaaggcaac cgtctttcac atattatcga cccaaaacag ttacgtccgg
    10561 taaaccataa tttagcctct atcaccgtat ttgccccaac tacaatgacg gcagacggct
    10621 tatcaaccgg tttatttgtt ttaggagcgg aaaaagcatt agcagtggca gagcgtgaaa
    10681 aattagctat ttttttaatt gttaaaaacg gcgataaatt tgagacacaa atgtcgagcg
    10741 aatttagcaa acttattcaa tcacaatagt tggagtgaaa aatggaaact gtattattaa
    10801 cctttggctt ttttgtagcc gtaatttttg cgatgtcgat tggttttatc gtgaaaggca
    10861 aaaccatcaa aggcagctgt ggcggtatca ccgctttagg tatgaaaaaa atgtgcgact
    10921 gcgaagaacc ttgcgataac ctaaaatcta aaatcgcaga cggcacagcc gaccctgaag
    10981 aagtggcacg ttttaataaa gagccacaat tttatgaagt gaaataatat gaatagagat
    11041 attcgctgga aacagcggtt tgataactat aaaaaatcag taagttacct tcaagcagaa
    11101 gcagaaaaat acgctgatac cgacattgat gtaattaaaa aaggaattat ccagagtttt
    11161 gagattacac acgagttagc gtggaagtta atgcaagata ttttaaaatg ggaaggtgaa
    11221 gttgatattt atggttcaaa aacggcttct cgtttggctt ttaatcgtgg gctaatcact
    11281 cagggcgaag tttggctaga aatgataaaa agccgcaata taacagttca tactgacgat
    11341 gaaactattt taactgaaga atttaataaa attatcaagg cgtatctgcc tttatttatt
    11401 gaatttgaac agcgagtaga aacactatgc caaactttgg actaagcgaa cgagcgacga
    11461 aaactattac cgatattctt gcgaaatacc ctgaagtgga gcaagccatt atttacggct
    11521 cgcgtgctaa aggtaattat cgtgaaggct cagatgtaga tcttactttt aaaggaaaag
    11581 atcttactta cgatattgtc aaacgcattt ggggtgattt agacgattcc gactcgccct
    11641 atttatttga cttgtcgatt tatcatcaat taaccaatgc caactttatt gaacatattg
    11701 atagagtagg aaaagtcttt taccaaagag aactctaatt tcttaatagg caagcggttc
    11761 attttgcaaa attttttgca aatttcaccg cttgttgttt ttattattaa ccaatgtgct
    11821 tcgccttaac cgaagttacg ggagatttat gacaactcaa ttaacctcaa aaacctacga
    11881 cactcatttc cctaaattaa ccccagaaca acttgctgaa aacagcaaga aaaaagtcat
    11941 tatcggtatg tccggtgggg tggattcctc ggtttccgct tttattcttc aacagcaggg
    12001 ctaccaagtg gaaggcttgt ttatgaaaaa ctgggaagaa gatgatgata cggattattg
    12061 taccgccgcc gccgagcttg ccgatgctca agcggttgcc gataaactgg gtatgaaact
    12121 gcataaaatt aattttgcag cggaatattg ggataatgta tttgaatatt tcttggcaga
    12181 atacaaggca ggaagaacgc caaatccgga tattttgtgt aataaagaga tcaaatttaa
    12241 agcctttttg gaatatgcgg cagaagatct tggtgcggat tatattgcga ccgggcatta
    12301 cgtccgccgt acgagcgatg atcaagatgc gaaacttttg cgtggcttag atgaaaacaa
    12361 agatcagagt tactttttat atgcgttaaa taaaaagcag atcgggcaga gtttattccc
    12421 agtgggtgaa attgagaagc cgattgtgcg agccattgcc gaagacctag gattagcaac
    12481 cgcaaagaaa aaagattcta ccggcatttg ttttatcggt gagcgtaaat ttaaagattt
    12541 cttagctcgt tttctaccgg ctcaaccggg ggaaatccgc accgttgagg gcaaattagt
    12601 cggtcgccat gatgggctga tgtatcacac gcttgggcaa cgcaaaggct tgggtatcgg
    12661 tggggtgaaa gggctaagtg aagatccgtt ttatgtggtg gagaaagact taattaacaa
    12721 tgtattgatt gtggcacaag gacacgataa ttcagccctg ctttcaagtg gcttaattgc
    12781 gaaacagctc acttgggtgg atatgcagcc gttgcgtgaa aatttacgct gcacggtaaa
    12841 aacccgttat cgtcaagcag atattgcgtg tgaaatccaa gtgattgatg atgaaaccat
    12901 tcgagtgatg tttgatgaac cgcaaattgc cgtgacgccg gggcagtctg ccgtgtttta
    12961 cttaggtgaa gtttgcttag gcggtggcat tattgagtca caattaaaat agttataaat
    13021 aaatattgaa atattttgca tatttatgca aaataagcga aaagttaagg cggtaaaacg
    13081 cctttttcat tttgatcgat acaaagaata atatacaagg aacagactgt ggcactttgg
    13141 ggtggacgct ttacacaaca agcagacgca aaatttaaat attttaacga ttcattacgt
    13201 tttgattacc gcttggcgat tcaagatatt gaaggctcaa tcggctgggc aaaagccatt
    13261 acttcggtag gtattttaac ggtagcagaa ttagaacaac taatcgcagc attaaaagag
    13321 gtgagagcag aggtagaatc taaccttacc atcatcttaa aagatgatgc ggaagacatt
    13381 cacagctggg ttgaatctaa gctgattgaa aaagtgggcg atttaggtaa aaaattgcac
    13441 accgggcgta gccgtaatga ccaagtagcg gtggatatta aaatgtggtg caaggtgcaa
    13501 gcggtggcat tgcaagaaag aatccgagct ttgcaagagc gtttggtttc cgttgccgaa
    13561 gaaaatcaag agtcggtgat gccgggctat acccatttac aacgagctca gcctgtgacc
    13621 ttcgctcatt ggtgtatggc gtattatgaa atgctagaac gggatttcag ccgtttgagt
    13681 gatgctaata aacgtatgag cacttgcccg cttggttcag gggcattggc tggtacggct
    13741 tacggcattg atcgtgactt attagcccgt gatttaggct ttgaagaagc cacacgcaac
    13801 agtttagaca gcgtgtcaga tcgtgaccat attttagagc tgctctctac tgcctcgatc
    13861 agtatgatgc acctctctcg ctttgctgaa gatttaatca tttttaatag tggcgaatca
    13921 ggcttccttg aaatgtcgga tcgtgtgacc tcaggctcat cacttatgcc acagaagaaa
    13981 aaccctgatg cttgtgagct gattcgtggg aaatccggtc gggtatttgg ggcattaaac
    14041 ggtttattaa ctacactcaa agggttacca cttgcttata ataaagatat gcaagaggac
    14101 aaagagggta tttttgatgc gttggaaact tggcaggctt gtttagaaat tgcagaactt
    14161 gtactggttg atattcaagt gaataccgaa cgcacccgag aagccgctca gcaaggttat
    14221 gcgaatgcga ccgagttggc agattattta gtggcgaaag gcgtgccatt ccgtgaagct
    14281 catcatattg ttggcgaagc ggtggtttac gcaatcagca aaaaagagcc acttgaagca
    14341 ttaagcatta acgaattcca gcaattccat cattcgattg gcgatgatgt ttacccaatt
    14401 ctctcgttag aatcttgctt agaaaaacgt tgtgccaaag gtggagtgaa cccacaacgt
    14461 gtggcagagg cgattgcggt agccaaagcc aatttagctt caaagtaaaa ccggatacaa
    14521 gcggtcatat tttgcaaaaa aattgaaaaa tatgaccgct tgccgattaa attttcaatt
    14581 ttttacgcat taagattgca aaataggcat tacctcttta aacttccgaa taatttttga
    14641 attttatttc tactactttc tttaaagagt tttatatgac ggaatcagtt aattctactc
    14701 aagctggtac agcactttct ttagggcaac aattaaaagc ggctcgtgaa gcgttaaatc
    14761 tctctattac agatgttgct caaaaaacca acttaaaaaa atcacatatt gaatcacttg
    14821 aaaatgatat ctttattcta caaaatgttg ccccaacttt tgtgcgtggc tatgtgcgta
    14881 attatgtgaa atttttgcgt ttacctgaat cccttatttc gagcgttaat tatggtgaag
    14941 tcactattcc aaaagaagtc actaaagttt cttcagtaaa accttcatca aattcacaag
    15001 gtaaaacctt aaagtattta acggtattag tattgcttgc ggcattagga atgacattac
    15061 tttggtggtg gcaagggtat caaaaagaac aggaaaatcg cgagcaattt gttagctctg
    15121 caccagttgc tgaagtggcg aataccgcta caaataatga tgcttcagca caaacttccg
    15181 tgcctgttca gcaacctatt caggtttcag agcagcctgc tcaagtagca gaaacggtag
    15241 ttgcagcaga accggcatta actgaaaaag tagagcctac acaagcagca tcatcacctg
    15301 taacacaaga agatgttaaa gtggtagaag aagttaaaca agctgatgcc accactgcac
    15361 aggttgaggc agttaatgtt ttacagcaat ctcaatcgga acaagcccct acagaacagc
    15421 ctgttgcggt aacgaatgat gagcttcgta tcgaaattac cggttcacaa agctggatta
    15481 cagtgcgggg tgctaaaaat aaacgtttag cagaaaaatt atataacaat ggtgagactt
    15541 taagtttcaa tgagaatgag caatatcgtt taaccattgg tgcgcctgcc aatgtaaaag
    15601 tgtactataa aggtcaagaa gtaccattga aaattgatgg gcgtgtagct cgtattcatt
    15661 taccgttaca gtaagcttga tttaacgatg aaacgcacgt aaagctagca tttaacgata
    15721 gctgtcttta cgagcgtttt ctgtttttat aaattttcac aattagctaa aaatatgata
    15781 catcaatcac ctattaaacg tcgggtatcg aagaaaatct atgtgggtaa tgtgcctgta
    15841 ggcggcgatg caccgattac ggttcagtca atgaccaaca cccgcacaac cgatgtggaa
    15901 gccaccgttg ctcaaattaa atcgctggaa cgggtcgggg ctgatattgt gcgtgtgtct
    15961 gtgccgacaa tggacgctgc tgaggcattt aaagcgatta aacaacaagt gagtgtgcca
    16021 ttggtggcgg atatccactt cgattaccgc attgcgttga aagttgccga atatggggtg
    16081 gattgcttgc ggattaaccc gggtaatatc gggaatgaag agcggatccg cacggtggta
    16141 gattgtgcca gagataaaaa cattccgatc cgaatcgggg taaatgccgg atcattagag
    16201 cgtgatttgc aggaaaaata tggcgagccg acaccacagg cattattaga atcggcatta
    16261 cgccacgttg atcacttaga tcgccttaac tttgatcaat tcaaagtcag cgtgaaagcc
    16321 tcagatgtat ttctagcggt tgaatcttac cgacttcttg caaaacagat tgatcagccc
    16381 ctgcatttag gtattactga agcaggcgga gcgagagccg gttcggtgaa atcggcggta
    16441 ggcttaggtc tgttgctagc ggaagggatt ggtgatacct tgcgtgtgtc attagcggct
    16501 gatcctgttg aagaggtgaa agtcggtttt gatattttga aatcgctgcg tattcgctct
    16561 cgtgggatca actttattgc ttgcccaacc tgctcccgcc aagaaattga tgtgattgcg
    16621 acggtaaacg agctagaaca acgtttagaa gacattatca cgccgatgga cgtttcgatt
    16681 atcggctgtg tggtaaacgg tccaggagag gcgttggtgt ctgatttagg ggtaaccggc
    16741 agcaataaaa tgagcggctt ttatttagat ggcgttcgtc aaaaagagcg ttttgacaac
    16801 gcaaaattaa tcgaccaatt agaagctaaa attcgtgcca gagttgcaca acaacataat
    16861 cgtatagata ttgaacaaat aggataaata tagtggcaaa aacaattcaa gcaattcgag
    16921 gaatgaacga ctgctcacca agtgagtctc cattatggca atgggttgaa cagaaagtgc
    16981 gtaatgtgct ggcaagttac ggctattcgg aagtacgtat gccgattgta gaaagcactc
    17041 cgttatttgc ccgtgccatt ggcgaagtaa ccgatgtggt gtcgaaagag atgtacacgt
    17101 tctgggataa cgatgagcaa ttaacgctcc gcccggaagg tacagccggt tgtgtgcgtg
    17161 cagcgattga gcgtggttgg atctacaata atgagcaacg cttatggtat atggggccga
    17221 tgttccgcca cgaacgtcca cagaaagggc gttaccgcca attccaccaa gccggtgttg
    17281 aagtgtttgg cattgcaaat ccggaaattg atgcggagtt gattattctc accgcacgcc
    17341 tatggaaaga gttaggcatc gatcaacatg tttctttgca acttaactct atcggctcgt
    17401 tagaagctcg tgcaaattat cgctcagcat tagtaaaatt tttagaaaat cacaccgctt
    17461 taatgaacga agaagagaaa gagcgtttgg tgaaaaaccc gctacggatt ttagatacca
    17521 aaaacgaagc actacaaaat gtattaaacg atgccccaaa actgctcgat tatttagatg
    17581 atgacagccg tgaacatttt acccaacttt gtgccttact tgatgcaatg ggaatccgtt
    17641 acgaagtcaa tccaaaattg gtgcgtggtt tagactatta caataaaacc gtgtttgaat
    17701 gggtcacttc agccttaggc tcacaaggca ccgtatgtgg cggtgggcgt tatgacggct
    17761 tagtcgaaca actcggtgga cacgcaaccc aaggcgttgg ctttgcaatg ggcttagagc
    17821 gtttagtgct gttggtacaa gaggtgaata aagagattga tttaccaaaa acggtggatg
    17881 tgtatatcgt attctccggt gaaggggcaa cactaaatgc cttccaactt gcagagcaga
    17941 ttcgtagcga attgccacac gttggcgtaa tgacacactg ttccggcggt aaattccaaa
    18001 aacaatttaa gcgtgcagat aaagtagaag cgcaaattgc gttagtgatt ggcgaaagcg
    18061 aagtggctga aaagcaagtt gttatcaaag atttacgttc aggagcagaa caaatcacga
    18121 ttgctcaatc agagctgatt gctgagctat ctaaacgatt ttaggagaaa aagatgagta
    18181 acgaatattt aaataaaacc gaagagcagc aatttgaaga tgccaaaaat tggtttaaag
    18241 agaacgggac accgattttg gtggcgatta ttttagcttg tgcggcaact gccggctgga
    18301 atttttggaa aaatcaccag cttgaaacgg cacaaaaaac atccgcttca taccaaagcg
    18361 tgatggagag ttatttgcaa aatccaagca aaaatcaacc gcttgttgag aaatttattg
    18421 ccgataataa agattcaagc tatgccgctt ttgcccaatt tgaagtagct aaacaagcgg
    18481 tcgaaaaagg gaatttttct gcggcaaaaa cagcgttaca gtcagttttg acttattcgg
    18541 attcaacctt acaatctatc gctcgttttc gtttggctca aattgacttc caactaaaat
    18601 cttacgatga agcactggca agtctggctc aaattcaaga taaagcgtgg gaattgcgta
    18661 agcaaattct aacaggcgat attcttgcag caaaaggcga tattcctgcg gcgaaaagtg
    18721 cttatgagca agctaaaacg accgcaaatc ctcaggaaca aaccttaatt gatgtacgtt
    18781 tgaataactt ataaaaacaa agcctgattg aaataaaatc aatcaggctt ttttgttggt
    18841 ttagtgttgc tgtgaattat aaacgcattg gcataatcac atattcggca gagctgttgt
    18901 cgcaatcttc aattaagcaa ctagaacttg aatcaactaa gctaaaacgc acacgttggc
    18961 attttaaggt gtttaacaca tctaatacat agctaacgtt aaagccgact tccatttccg
    19021 ggctgtcgta ggcaacatca atgatctctt ccgcctcttc ttgctccgga ttgtttgcgg
    19081 taattttgag taaattattg cttaaggtta aacgcacccc tctaaatttc tcattagaaa
    19141 gaatcgctgc acgcattaat gcccgtttaa gggtttcggt ttccgcttct agaatgcgat
    19201 ctgcattgcg aggcagaact cggcggtaat ccgggaatct gccatcaatc agtttagagg
    19261 taaatacaat gccgttcatt gtaatacgga tattgcttga accgatttcc aaacgcacgg
    19321 tttcatcgtt gctggcaacc aagcgggaaa gctcaagtac ggctttgcgt ggcacgataa
    19381 ccgaatgagt caaaagctct tgatttagca ccattgtgca aaccgctaaa cggtgcccat
    19441 ctgtggcaac ggtgcgtaat aaattgcctt cggtttcaaa tttcattccg tttaagaaat
    19501 aacgggcgtc ttggtttgcc attgagaatt gggtggcatc aatcagacgg gcaagggtag
    19561 attgatcaat cgagaaatcc acttccggtt tccaatccat caaattcggg taatcacttg
    19621 ccggtaatgt agctaaacta aatttactac gtcctgcttt gataatggct ttatcttctt
    19681 caaattgtac cgaaatcacg ctgtcatcag gtaggctttt actgatatct aggaattttt
    19741 tagctggaat ggtaaatttt ccggcaaaat cgaccgcttg tagcagttgt gcttgggtgg
    19801 ttaattccac ttctaaatcg gtaccggtta aagatagacg attaccttca atttctaata
    19861 aaagattgtt aataacaggc aaagtcggac ggctacttaa tacgccacac acctgttgta
    19921 aaggcttaag taattgttct ctggtaatgg taatttgcat aatcattcct tcatcgggct
    19981 aagatgataa tttgcgtgtt aaatggatat aatcttcttg gataccgctt tcggtatcgc
    20041 gtaattcatt aatggttttg caggcgtgca tcacggtagt atgatcccga ccgccaaatt
    20101 cccgaccaat ttccggcaag ctgtggttgg ttagctcttt tgccagtgcc attgccattt
    20161 ggcgaggacg ggcaatgctt cttgcccgac ttttcgattt aatatcggca acttttatgc
    20221 cgtaatattc ggccacaatg cgttgaatat tttcaatcgt aatcaagtaa tcaaaagagg
    20281 caaacagatc ttgcaaggtt tcacgcaccg catcaaccgt aatcgggcga agggtcaatt
    20341 tttgccacgc aatcacacgg tttaatgctc cctccaattc acgcacattg gttcggagtt
    20401 tttgccccat taaataggcg gcatcttctt gcaaatctgc tccacgttct aaggcttttt
    20461 ggcgaagaat tgccactcgg gtttccaatt caggtggctc aatggtcgca ctaataccgc
    20521 cgcttaaacg ggatttaatc gctgtgtcaa ttttatcgat attttttggc aaaacgtcag
    20581 aggttagaat aatctgcttt ctctgctcaa ataaagcatt gagtagatgt aaaaactctt
    20641 cttgtacttt cggtttattg gcaagaaatt gaatatcgtc aatcattaac acatcaagcg
    20701 agcggtagaa ttttttgatt ttttccgcat cgttattgga actgttgatc gccttaataa
    20761 tatcacgata aaaacgttcc aaatgaatat atagcacccg agtacccggt gtgcgtttaa
    20821 ctaattcatt gccgatagcg tgtaataagt gggttttgcc caaaccggtg ccggcataaa
    20881 acgagagtgg gttgcaatgg ctttcgccta aattatccac cacttgttga gccactaatt
    20941 tagctaattg gtttgagcga ccttgtacaa aattatcaaa ggtttgattt ggatttagcc
    21001 cggttttaaa gccggtattg ctgaattctg aactctcgtt ttttagattt gggctgacct
    21061 ttggctgttc tgtcggttta atcccaactt ttaacgtaac caccaaatta tcatttttcg
    21121 ccaaaaaacg ggcaagctcg gtaatttccg ataaaaattt atcttttacc cagttttcaa
    21181 caaattgatt ttgtgcataa agtgtaagtt ccccattctc agaactcgct tgcagaggac
    21241 gcagccaagt gctgtaatca gttggtgaga ctttcgtttg taagtgatta aggcaatcag
    21301 accaaaggga agacacaatt tgctccaaaa ttattgttat taaaggtcat aatgatacct
    21361 gaaaaaaggg gagaaatctt gcaaattttt gagagatttt atgggggctt ggaagcgatt
    21421 ttaattggcg aacaagcggt cgttttttgt tgaaatcttg caaattgcta acaaaaaatg
    21481 accgcttgct tagtatgatg tgtgatggag ttgattaaat atccgtattc tccggcaaga
    21541 acccaccgct ttggtgagcc caaagtctgg cgtaaacacc gttttgagca agcaattctt
    21601 cgtgcgtgcc ttgttcaacg atttcgcctt tatctaacac gataagtctg tccattgcca
    21661 taatggtaga gaggcggtgg gcaatcgcaa ccacggtttt accttccatt aattgcgtga
    21721 gattttcttg aattgccact tctacttcag agtctaacgc actggttgcc tcatcgagta
    21781 ataaaatcgg agcgtctttt agcatcactc gggcaatcgc aatccgttgg cgttgtccgc
    21841 cggaaagttt tacccctcgt tcgccaacgt gtgcatcaaa accggttctg cctttggcat
    21901 cttgcaggtt tggaataaat tcactggcgg cagcttgttc aacggctttg atcatctctt
    21961 cttcagtggc gtttgggcga ccgtacatca agttttcacg cacagagcgg tgtaacagcg
    22021 aggtgtcttg agttactaaa ccaatttgtg agcgaaggct ttcttgggta atatcacgga
    22081 tattttgccc gtcaatcgtg attgaaccgt cttgaatatc gtaaaaacgc aataataaat
    22141 tggtgagcgt tgatttacct gcaccactac gcccgactaa gccgactttt tcccccggtt
    22201 ttatagtgag gtcaaaatct ttcagtagcg gttttttcgg atcgtaggca aaatccacat
    22261 tctcaaattt aatttcgcct ttggttacat ttaacggttt cgcatcgatt ttatctacca
    22321 cattgtgtgg tttggaaagc gtaatcatac cgtcttgtac cgtacctaaa ttttcaaata
    22381 gacgagcaaa ttcccacata atccattgtg ataagccttt aatgcgtaaa gccaaagcgg
    22441 ttgaggtggc aatcgcccct gcagttacta cgcttgaacc ccaaagccat aaaccgatac
    22501 ccgcagttga aattattaat gccatcgagg ttagattagt tacggtttca attacagtca
    22561 ctaaacgcat ctgtttatgc actgtcacca taaatccttc catcgactct ttcgcatagg
    22621 cagattcacg gttgccgtgc gagaagagtt tgacggtggc aatgttagcg taagcatcgg
    22681 taattcgacc ggtcattaaa cttctggcat ccgattgctc ttgagcagct tgggctaatt
    22741 tcgggacaaa gtagataatg gttgccgcta atgagatgac ccataaaata aacggcacga
    22801 ataaccagcc gtcaagttgt aataaaatca cgctggaggt ggtgaagtaa accagcacat
    22861 acaccaacat atcggcacaa gtcataatca catcacgcac ggaaagggcg gtttgcatta
    22921 cttttgcaga cactcttcct gcaaattcgt cttgatagaa acctaaactt tgcccaagca
    22981 ttaagcggtg gaagttccaa cgcaaacgca ttggaaatac cccttgcaga gattgaaagc
    23041 gaatgctgct ggcaaggtaa acaaacacca ctgcaagtag ggcaataaaa aacataccga
    23101 taatcgtcca gcctttttct gcccaaagtt ctgttggcga gtatttattg acccaatcga
    23161 ctaaatcgcc cataaagcga aacagaaccg cttcgataat accgacagct gcaactaata
    23221 cggtcagtag taagatataa aaccgcattc ctttagtggc atcgtaaata aaaggaatga
    23281 gacctgattt tggagttttc ggcatttcat ttggataggt ttccaccctt gcctcaaacc
    23341 atttaaacag tttttcaaac ataaatttgt gatttccgaa taaaaatgca ggggcaaata
    23401 gccatttgcc cccgaaaaaa tgtgaaaaaa ttatggatta agtaattttt cgtctaaggc
    23461 taaatcttcg ttgcgattta taccaatgcc ggcagctaat acgttgcggg caatttgatg
    23521 tgcttctttt aaagaatgct ctttgtaaga gccacattga tattcattca gctcagggat
    23581 tgccgataca tcaggaattt tatgtaacgc atcttccatt gatgccgtcc aagctttgac
    23641 aacttcttct gctgaaggcg atccaatcag tgacatataa aaaccggtac gacaacccat
    23701 tggtgaaata tcaatgatct ccacttgttc gctattgagg tgatcacgca taaaaccggc
    23761 aaaaagatgt tccatcgtgt gaatcccacg caccggtaga atatcaatat tcggacgaca
    23821 gaaacggaga tcgaacacgg taatggtatc gcctttcggt gtggtcattg tttttgcaac
    23881 acgcacggct ggtgcgttca ttttggtgtg atctacttta aaactatcta ataaaggcat
    23941 tttttactcc atcaatatta ttccgcaatt ccttgatccc aagccggggc ttcatcaggt
    24001 agtttgcgct gtaagaaaaa tcgtctgttt ggctgggctt gaggcttcac cactttagtg
    24061 taaggggtag agaactcaat accgccattt aataactgcg acattgtgcc ggagttcata
    24121 cttacacctt gcaggctcac gtccatagtg taacctgaag cttgccagaa ttgggtattg
    24181 gttctgacta agtgcttgta tttatcttga atattgacat aaataagcac tcgatcgcct
    24241 aactcactta gccctaaacg gctgactatt cccacctgca ttccacggta aagcaccggt
    24301 gcttccggct gaataccgtt agcattggtg gtttcgacaa taatggggaa accgttggca
    24361 taggtggttt ttaccgtatc ggtatctgca agaacaaatt ggttttgagc ttgccctgaa
    24421 ccgacatcaa ccgtaatgta attttgtagt gctgcatcta aatttttaaa gccggaggta
    24481 ctgatttcag gcgagataac attaaattta ctgccggctt ttgcaattaa cggatagtat
    24541 ttcctttgaa tgtaagcggt cgcttttatc tgtttttttg cattttgtag ctcaagtttt
    24601 tcaatttgac ctacggttaa gcccatatat ttaatgtcca ttccttcgct taacttactc
    24661 gcatcttttg caatcagcgt gagataagta ttgcccgaag tggctttctc cttacttgca
    24721 taaagcgttt tattgccttt tgaaccgcca tcatcaaagc tgatcgcccc ttttaaggta
    24781 cgcattaatg gagctgtagt gatattcacg cctttgagtg acacatcaac actcgttgcc
    24841 ggctcaatcc agaaacggct tttctcggtt aatagatgac gataatttgg ctggataaat
    24901 agctcaacct caaatttttg ttgtttcggg gttattttta acacctcgcc gacctgaaaa
    24961 tcacgatata gcaccaaaga gccttcgctg ataccagata atgaatctgc cgaaagggta
    25021 gtggtggctt ttttatctaa ggtagccatg cctgattccg cactttctat atctttataa
    25081 agtgcatatt gttttttcgg gctaccgtca tttttaccgt taattaagcg aacaccgcct
    25141 tttaaccatt cagatggtga accggcgtga actcttaacc catctagccc caccgacata
    25201 tcaatgttgg atatcgcaac aaatttgctg ttactcccaa tcaggttgcg ataaggcgga
    25261 taaataatga catcaaaacg tactttgtct aactctaggc tacgtttgac gatttctccg
    25321 atttgcacat cgctatagta aatcccctga ttggtatcta ccccataggc ttgaggagca
    25381 attaagctaa agctaagtaa attcggtaaa cttaatagat aatcggcttc gttttgtacc
    25441 acaaattccg attgaggttc tccttccccc gggtgaacct caaaatagac accacgcagc
    25501 agctcaccta ctttcgtcag ctgctctgtg tttaaggaga atttcggctc tttgagcaag
    25561 attttagttt gggaacgtag taaattttca taacttgggt caattaataa aaccccatca
    25621 atctgtttat gctgccctga ttcttgctgt ggcacaccgt tgtcttttac ttgcggtaat
    25681 tttagctgcg aaagcatacc aatttgcaca ttttggtgaa aaacaccagt ctcattaatt
    25741 tttaaattat gtgtaagcgg tagttttacg ttgatttcaa ttccacgttt agctgatttc
    25801 aaatccggat atagctcaaa tttctgtcct tgctcggcaa tttccatatt atcaggcgaa
    25861 tcaaaagcaa ccgcaccttg tactaccgag gcaaggctgt ccatattgat tttaatacct
    25921 tgtaaattta catcagcgct aataccgcta atattccaaa atcggctctc tttttttact
    25981 aaatgagcgt atttttgatt aatgacgaca tcaatttcga tttttttctg gtctttggta
    26041 aaacgataat cggcaatatt acccactggc actttacgga aataaacact cgctccgaca
    26101 gtaatagagc ctatgtcatc agagattaaa cggatcagta aatcgccatc gctcacggta
    26161 atagccggag gatcttcttc tgcaataaat tcatcaacga aatcgccttc accgggaagt
    26221 agggtaatat agttaccaga aacaatcgca tctaagcctg aaacgccagc caaagaagca
    26281 cttggttgca ctaaccaaaa tttagtctgt ttgcgtaaaa tacttttggc ttcagggttt
    26341 atttctgctt gcacttccac ttcttttaaa ttatcgacaa aataaacacg cttaacctga
    26401 ccaatctgca agccttggta acgaatagcg gtttttccgg cagtaatgcc atcaccttta
    26461 ctaaatcgaa tcgtgattgt ttcaccttgc tcttttaaaa tttggaaaaa tagcaaagca
    26521 ccaataataa aagcaactat tggtaacagc caaaaaggag aaattttgcg aggctgccgg
    26581 atttgggcat ctacaacagt gtgggtattt tcttccatcg tcattttaaa ttagggaaac
    26641 ctatatagaa caataccgaa gacccaaagg gacttcggta atagaaataa gtcaaaataa
    26701 gaaatattat tttttctcat cacagttggt taaattactg catttaccat acagatataa
    26761 actgtggtta gtcaattcca ttccgtgttt ttcactgatt tcacgttggc gacgctccat
    26821 atcaggatct ttaaattcga acaccttacc acaatccata caaataatat ggtcgtgttc
    26881 gtggttcata tttaattcga atactgcttt attcgcatcg aaattatggc gggttaaaat
    26941 gccggcttct tcaaattggt ttaagacacg ataaaccgtt gctaaaccaa tgtctgagcc
    27001 tttttctaac agaatcttat aaatatcttc tgctgcaaaa tgttgcattt catcacgaat
    27061 atcttgcatt aatgcaagga tagtcaaacg aggctcggtt acttttaagc ctacctcttt
    27121 taaacgttta atatttgctt cagacatata tgccacttat ttatctttat ccaaaatagt
    27181 tattatgcga gttggtctaa gcacatttct tctttcaact gctcgcacca tttattaaca
    27241 cgttcagtgg ttaattcagg ctgacgatct tcatcaatac ataagccgac aaaagtatca
    27301 tcatcaacta aggcctgaga aacctcgaaa gtgtaaccgg cagttggcca atgaccaaca
    27361 atcacaccac catttggctc taccacatca cgcacagtac ccattgcatc acagaaatat
    27421 tctgcataat cttcctgatc accgcaacca aaaattgcaa cgactttgcc gttaaaatca
    27481 atctctttta gggtaggcat aaaatcgtcc caatcggctt gcgattcacc ataataccaa
    27541 gttgggatcc caaacatcag aaaatcgtat gcttcaatat cttcttttgt gcttttggca
    27601 atgtctcgaa tatcaattaa ttcagcccct aattttttct gaatcatttt tgaaatattt
    27661 tctgtattgc ctgtatcgct accgtaaaat aaacctacaa ctgccattgc tactacctat
    27721 ttgtgagata attatttaga attgtttcaa ttaactgccc gcgtgaaaca ccttgttcag
    27781 acgcctgttc ctctaataac tttactaaat tcgaatgcag ttttaattct acccgtttta
    27841 aaccggaaga tttatctcgt tttagttggt tacgtttatt gattcgaagt tgttgttctc
    27901 tatcaagagg atttgtacga ggacgcccaa cttttggtac agtcgcaaac agatctaagg
    27961 ttatacaatc tgaatcttgt tttgccattt tgttttacta ttgggcttta gctaatcctc
    28021 agttttaatg agaatgataa gctactttta aagtgtgaac tatatcaaaa gttcaatcat
    28081 ttgaaaagta aattacaggc aaaacaagcg gtgtttttag gtttaaaatt tgcaaaaacc
    28141 ataaattaca cattatgagt tatgattttt ctgaagcgtg aaattagctt ataatgaaac
    28201 cgaataacgt tatgaaaaag gagatagtgt gtcgaatatc catttagcga ttgccgctga
    28261 ttttacgctg gcagaaaaat tacttgaagc attagcaaat caagatttag ggattgaaaa
    28321 tatttcagcc gttgagcttg agccgtttgg tgaagagcag aatttacgct ttggggcaaa
    28381 agcagtcgca caatatgcgt tagatgaagt aaattgggcg gaatttagcc atgttctatt
    28441 tgccggcaaa atggcacaag cggaagtgtt ggcagaagcg gtgaaagccg gctgtgtgat
    28501 tttagattta tacggcatta ccgcactcat cggcgatgta ccggtagttg tgccaagtgt
    28561 aaatgatgaa aatgtcgcaa atttgcgtga gcgaaatatc gttgctttag ccaatccgca
    28621 aatttcacaa ctggcgttag ccttaaagcc attgttgaac gagccgttaa aacaggtgtg
    28681 ggtaacctca cttttaccgt ctgcctattt tggcgatgaa aaagtcaagg agcttgccgg
    28741 acaaactgcc cgtttgttaa acggcattcc gtttgatgaa aatgcggaac gcattgcctt
    28801 tgatgttgtg ccggctaatg tgcaaggaga agagaaaagt ctgccgttct caaaagcctt
    28861 tgaactgcaa attgcaaaag ttttaccaaa tttgaccgct tgtactacct tccacagcgt
    28921 gcaagtgccg gtcttttatg gggtttccca aatggtgtcg gtgcagtctg aatatcagtt
    28981 agatgctaat gcgttaagtg aagcttggaa agccgaaaat tggctgaatt ttaatgaaaa
    29041 ttcagtgatt acagcggtga aaaatggcga aaacgaagag caaaatgcgt tagaaatcag
    29101 tcagttattg gcgaaatccg aacaggaagt gcagttctgg acggtcgccg atgaacagcg
    29161 tttcagtctg gcatttttag cggtgaaatt actaaaatta gttttagcgt attaagtgct
    29221 tgcaaaagta tttattccgt gataaaaatc gtcaacgccc gagcaaccga ttgcttgggc
    29281 gttgtcattt tgtttaaaca ccgaaggtct tatgttagaa aaattatttc agctcaaagc
    29341 aaaaggcagt aatgtaaaaa ccgaaattat cgccggtatt actacctttt tcacaatggt
    29401 ttatattgtt ttcgtcaatc cgtctatttt aggcgtggcg ggaatggaca cccaagtagt
    29461 gttcgtgact acttgtttaa ttgccgcttt tggtactatc gcaatggggt tattcagtaa
    29521 tttaccgatt gctcttgcac cggcaatggg cttaaatgcc ttttttgcat tcgtagtggt
    29581 gcaaaaatta ggttactcgt ggcaagtggg tatgggggcg attttcttag gctctgtcgg
    29641 cttattccta ttaactgtat tacaagttcg ttactggttt atgtctgcga ttccacttgg
    29701 tttacgtgta ggtattggtg ccggtatcgg tttattcatt gccctaatcg gttttaaaaa
    29761 tatgggcttg gtggtgccaa atccggcaac gcttgtggca ctaggtgatt tgcacgaccc
    29821 gaaagtgtta atggggattt taggcttttt catcattgtt gtattagcgg cgaaaggtat
    29881 tcactcaggc gtgttaattt caattgtgat tgttaccgct cttgccttag cctttgaccc
    29941 ggcggtgagc tttaacggca taatgtctat gccaccaagc cttggggcag ttgtggggca
    30001 ggttgatatt gccggtgctt ttgatgttgc cttactcggg atgattttct cctttatgtt
    30061 ggtaaacctg tttgactctt ccggcacctt aattgcggtg actaccaaag caggctttgc
    30121 ggatgaacaa gggcgtttcc cgaaaatgaa acaagctctg ctagtagata gtaccgctgc
    30181 aatggcaggc tcttatatgg gaacttcagc gatttctacc tatattgaaa gtggctccgg
    30241 cgtttcagtt ggcggtcgta ccggcttaac tgcggtggta gttgggatct tattcctatt
    30301 aaccattttc ttctcaccac ttgcagggtt agtacctgcc tatgcaactg cgggggcatt
    30361 ggtatttgta ggaattttaa tggcatctag cttaattgaa gtaaaatggg acgatttaac
    30421 tgaagctacg ccggcattta ttactaccgc aatgatgccg tttacttact ccatcactga
    30481 agggattgca ttcggcttta tcagctactg cgtaatgaaa gcctttacag ggcgttggaa
    30541 agaaatcaac ctttctgttt tagtggtttc acttctcttt attgctaaat ttgtgtgggt
    30601 gggttaagct ctacccccct ctccctgatt tttcttctaa acgaagaaaa atctatccct
    30661 ctcccacaaa ggggagaggg tatttgattc attatctatg tgtttcgtgt attccgaggt
    30721 aaaacaatgt ctaattcagt tcaaaaactt ctttttatca tcaattctgc tccttacggc
    30781 aatgaaacgt ttttcagtgc gttgcgtttg gcattacaaa ttcaagaaca acataaagcc
    30841 aatacggaaa ttaaactttt cttaatgtcg gatgcagtca ccgcagggct ggcaaagcaa
    30901 aatccggcag agggttatca tattcagcaa atgctggaaa ttttaaccgc ccaaggggca
    30961 aacgtaaaat tatgcaaaac ctgtactaat gctcgaggga ttacagattt gcctttagcc
    31021 gatggggtag agattggcac attaattgaa ctggcagatt ggacaattca agcagataag
    31081 gtactgaatt tttaatgcaa aatattgatg ttgtggtgat tggtgccggt gcggcagggc
    31141 tgttttgtgc tgcccaactg gggcaagctg gcaaaaaagt ggtggtgtta gacagcggta
    31201 agaaagtcgg acgtaaaatt ttaatgtctg gcggtggttt ttgcaatttt acgaatttag
    31261 acgtaagtgc cggaaattac cttagccaaa accgccattt tgtgaaatcc gccctttcac
    31321 gttataccca atgggatttt atttcgcttg tcagccacta tggcattagc tatcacgaaa
    31381 aagagctagg gcagttgttt tgtgatgaaa gctcgcaaca aattgtcgat ttactacaag
    31441 cagaatgcaa caaaggcaag gttgagattc tgttacggca agcggtcatt tctgtggaaa
    31501 aagttgcaaa tttttttgaa atttcgaccg cttgtggcaa gttccaatcc gaacacttag
    31561 tgattgcaac cggagggctt tcaatgccgg ctctcggggc aagccctttt ggctatcaac
    31621 ttgccgagca gtttggtatt cctgttgtgc caccacgtgc aagccttgtg ccttttactt
    31681 ggaaagagag cgataaatat tttgccgagc tttccggcat tgcactgccg attactgttt
    31741 ccgccaatgg caaaagtttt agcaatcaga tgttgtttac gcacagaggc ttatccggcc
    31801 cggcaatttt gcagatttcc aattattggg aattggggga aacggtagag attgatctgc
    31861 taccttcgca gtccattacc gatatattga gcgaactacg ccaatcttcg ccgaaattac
    31921 agctaaaaac cgtattatct cgttatttac ctaaaaaatt ggttgaagtt tggctggaac
    31981 agaaattgct gaccgataaa gtgattgccg atttgagtaa aaaagatctg accttattag
    32041 agcaatttat ccaccattgg catttcttgc caaacggcac ggaaggctat cgtacagcgg
    32101 aagtaaccaa aggcggtgtt gataccgact atatttcatc taaaacgatg gaagcgaaaa
    32161 atacagcagg gctttatttt atcggcgaag tgctggatgt taccggctgg cttggtggtt
    32221 ataatttcca atgggcgtgg tcatctgctt ttgcctgtgc agaagggatt attgaaaaaa
    32281 gcaaataact aaaaaagcaa ataactaaaa aaccaaacaa aaaggcgaac acaattgttc
    32341 gccttatttt atgctgaatt atcttgcacg gaaaataatg cgtgctttgc ttaaatcgta
    32401 tggagtcatt tcaaccgtta ctttatcacc ggttaaaata cgaatgtagt ttttacgcat
    32461 tttgcctgaa atatgtgcgg taacaatatg accgttttct aattccacac ggaacattgt
    32521 atttggtaag gtttctaaaa tcgtaccttg catctcaatg caatcttctt tagccattta
    32581 ttcctcttta aagttttaat ttttatatga tgccattcgg cgaaaaacgg cggatattat
    32641 acgcttattc gctcaaatta caatcgttta tccatttgag tgtaaactat ttacgcttaa
    32701 tccaacggcg acgtgagtga aaacgctggg tttgggtttt tacttgactc ataagtggtt
    32761 ttgggcttat ctgtacatga tgaattttac gacgaaaata gtagttaatg ctacttgcca
    32821 acaatatacc tgtgagtagc aaaataatta tttccccata aagttgatga aattgttgct
    32881 ctactcgtgc tttagtagtt tgcccatatt ctgcatcaaa cgtgacgcgt aaaaagccaa
    32941 ccaacgcctg ttgagaaaat attggctcaa caatttgctg agttttggca gattgctctg
    33001 aatcgagcaa tacttttaaa tctttcgcat tttggctttg agccagtagc acgccatttg
    33061 gtgaatagat attggcatca ataataaaat cttctttggc aaaactatcc aacgcatcaa
    33121 ccagctcatc ggttttggca tttttaacta acaaaaacga aaaaatattt gcttgctggc
    33181 gaaccaatag gtgtgaaagg tttgatactt gattaatgct ggccaattgt gagcctagct
    33241 taaattggtt tacgcccatt aaaataaccg ccacgatccc acagcagagt agcgtaatcc
    33301 ctgccagcaa aagcgttttc gttatttttt cacgactaat gtacatcgaa attccctcaa
    33361 aatgatgcgt gattgtagca cgcttttctg ttagattagt gcagtaaatt taaacaacct
    33421 aaaataagac aatgacaaac acaattttta tctattcaaa aaccttaaac gagcgtgaaa
    33481 ttgaacagtt ttcacaacaa acggcagcaa ttttgctgaa acaagcggtc tatttagact
    33541 acaaaattgc atttttttcc acaaatttaa ccgcttgcga gctgcgtgaa acggcacaga
    33601 aaatcaatgc ggatattagc gatctcacta ttattccaac cttaaaccaa ggcggtttgc
    33661 tattaatgga tatggattcc accgctatta aaattgaatg tattgatgat attgccaaac
    33721 ttgccggcac cggcgaggtg gtgtccgcca ttactgccag tgcaatgcgg ggcgagctgg
    33781 atttcgagca aagtttacgc aagcgagtcg gcacgttgga aaatgcatcg gaaagcattt
    33841 tgcaaaaagt gcgggaaaat ctaccgctta tggacggctt tgagcagatg gttgctattc
    33901 ttaaggaaca cggctggaaa ttagcgattg cgtccggtgg atttgattac ttcgccgatt
    33961 acttaaaaca aaaatacggg ttgaattttg cggtctctaa ccaactggaa attattgacg
    34021 gtaaattaac cggcaaggtg ttaggcaaag tagtggatgc tcaacataaa gccgatacct
    34081 tacagaattt agcgacaacc tttaatattc caccaaatca atgggtggca gtcggcgatg
    34141 gggcaaatga cttaccaatg ttaaaaaccg ccagtttagg cgttgctttg cacgctaaac
    34201 caaaagtgca agcacaagct gattttgtgg taaacttcgg ggacttaacg gcattatgtt
    34261 tattacttaa tgccaatcat ttatttcaac attaattgag aggaacaaca atgccatcat
    34321 ttgatattgc atctgaaatc acgatgcacg aagtgcgtaa tgcggtagaa aatgccaatc
    34381 gtgtattaag cacccgctac gatttccgtg gggtggaagc cgttattgag ttaaatgaaa
    34441 aaaatgaaag cattaagctc acgaccgaat cggatttcca attagagcag ttaattgaga
    34501 ttttaatcgg ctcttgtgta aaacgtggga tcgagcatag ttcattagat atccctacag
    34561 aaagtgagca tcacggtaaa ctctattcca aagaaattaa gctcaagcaa gggattgagt
    34621 cagatatggc gaagaaaatt accaagctga ttaaggattc taaaatcaag gttcaagccc
    34681 aaattcaagg cgagcaagtg cgtgtaacgg gcaaatctcg tgatgattta caagccgcaa
    34741 ttcagctagt gaaaggggca gatttagggc agccattcca atttaataat ttccgtgatt
    34801 agcaattcct gcaattaatg atgacaagtg gaaaaattca gcaaaatttg accgcttgca
    34861 ttaataattc aacataaaag gaagagatga tgagcgagca aacagaaaag aaaggctggt
    34921 ttaaaaaagc ccttgagaaa tatgatgcct tttgcgatga gctaggggta aatcaaggtg
    34981 cttgccgagg ttgtgtccct gtggtgaagt ttgaccctga accggaaaaa gagcagccag
    35041 aaaaatcaga aaataaaaca tagaaaaaat gcacaatggt aagatacatt gtgcattttt
    35101 tataggattt ttaattggcg tgatgatggg caaaatattg cttaactccg ccatctttct
    35161 tgcaaggatt ctgaatatca gtagagtaac taccgttaaa acaaagagca gtagcattcg
    35221 atggtttaat cttttcctgt ttagttaggg ctggcggttg agtattggtt tcactaatat
    35281 agatcgtttt aggttgagaa tagtctatat ttttgtcgcc atttacgact tgaacggggg
    35341 tgttcgagct gcaagctaca accgatgctg aagccaatgt aagtataatg atatggtgtt
    35401 ttttcatggg taatttccgt ttttatattt ttaattgaga gaatgttaaa aagggctgat
    35461 ttctcaaccc ttaaagttat gccttaagca tttttattaa aagtttgtcc taactgaatt
    35521 tcatcattta atacaggaat taactgctct agttgatctt tttcaaattc gctgagattg
    35581 ccgataggta gaactttttc cacaccattt gtacctaaac gaatcgggta agcaaagaat
    35641 gccggagagg tttcgccatt ggcattatct acataagcat aacgaatgac atcttccccg
    35701 attaaggctc tcactaaacc aagtgcaaat tctgctcctg cttgagccat agataaggtt
    35761 gctgagccac cacctgcttt tgcctcaacc acttcagtac cggcgttttg gatacgatga
    35821 gttaattgct cgatttgttc ttgagtgaac tcaagtttta agccatttac tgttgcttgt
    35881 gaaagcagtg gaagaatagt tgtgccggaa tgtccgccaa tcacaggcac tcttacatat
    35941 ttcacattta cgtctttcgc ttcagccaca aaggtattcg ctcgaataac atctaatgtt
    36001 gtaacaccga aaagtttatt tttgtcatac acaccggctt ttttcaatac ttctgccgca
    36061 atcggaataa tggtgtttac cgggttagta ataattgcaa tgcaagcttt agggcaaact
    36121 ttcgctgctt tttcgactaa gttatgcacg atagtggcat tcgtattgaa taaatcggca
    36181 cgtgtcatcc ccggcttacg ggcaacacca gcagaaataa cgaccacatc ggcatctttt
    36241 agtgcctcag tcggatcttc acctgcaaaa ccggttactt ttacatcagt tggaatatgg
    36301 cttaaatcca ctgcaattcc tggagtaaca ggggaaatat cgtaaagaga taattcagat
    36361 ttagccggta aattgagttt taataataag gcgagtgatt gaccgattcc acctgcagca
    36421 cctaaaaggg caactttttt actttgacac ataaaatttc tcctaaatgt tatggaagtt
    36481 tagaccaatt tagtctaagc caattttttg ggcgattcaa acggcaaatc tcatttttga
    36541 gacatagagc aaatttttag ctgattttta tgggttttat tgatttattt gcataaataa
    36601 gcaagaatta tgaatatttt taagtttttg tgaataagag tgtgaaagtg gacaagatag
    36661 atagtttatc agaagcgttt aaatcgctgt taaaggaaga aaaattcggc tcgcaaagtg
    36721 aaattgttgc cgcactacaa gagatgggct ttgataatat caaccaatcc aaagtttctc
    36781 gaatgttatc aaaatttggt gcagtcagaa cccgtaatcc taaaatggaa atggtgtatc
    36841 agctaccagt cgaattaagt gtaccgacta cctccagccc acttaaaaat ttggtggtag
    36901 atattgatca taatgatgcg ttaattgtgg tcaaaaccag ccccggtgcg gctcaactga
    36961 ttgcacgttt gctcgattca atgggtaaag ccgaaggtat tttaggcact attgctggcg
    37021 atgatacgat ttttataacg ccggtacgca atgtgcaaat tgaaaaattg gtgaagaatg
    37081 tgacacagtt atttgaaaca tcattataga ataagtaaag gaggacttaa tgttctcctt
    37141 attttttagg agattattat gcacattttt ataacaggtg gtacgggttt tattgggcgt
    37201 gaattaatcc agcaattaaa atctgaaaat caccacttta cacttcttac tagagataca
    37261 gaagcgaaaa gtgatgattt acagattaat tattgctatt cacttgatga acttgttgat
    37321 ttaaatgatt ttgatgcagt aattaacctt gccggtgagc cgatttttga taaacgttgg
    37381 acagacgaac aaaaacagaa attgattgaa agccgggtta acctaacgaa taagctcagt
    37441 caattaatga atgcaagctc taaccctccg catacttttc tctccggttc ggcaatcggg
    37501 atttatggcg atttgcctcc ttctgcaaat ttttatacag aatcgaccgc ttgttatcaa
    37561 gattgcttta ccaaccggct ttgccaacag tgggaagccg ctgcactaaa agccgcaagt
    37621 gataaaaccc gagtgtgttt aattcgtacc ggcacggtat tttcgcccga aggcggagca
    37681 ttaaagaaaa ttttaccgct ttatcgatgc ggattaggcg gaaaacttgg ttcaggcaaa
    37741 caatattggg cgtggatcag ccgccgggat cacgtacaag cggtcatttt tctgctcaaa
    37801 aatgcaaatt gttcgggtgc ttataatttt gttgcaccaa atccggtgag aaatgaagaa
    37861 tttaatcgta ttttagcgaa tgctgttaat cgcccggcat tctttactgt gcctgaattt
    37921 gccttgaaat taggcttagg cgaacgagct tgtttagtct tagataatca acagctcatt
    37981 ccggaaaaac tactcgcagc cagttttgta ttccaagatc cgattttaac cgagaacgtt
    38041 ttcttaaacc cataatttag gctataatgc tgtataaaag cacagattaa aaggtgatta
    38101 tggatttctc attattatta gacggtttaa atgacaagca gagggaggcg gttgctgccc
    38161 cgattggtaa ttatttagtg ttagccgggg cgggttcggg caaaactcgg gttttaaccc
    38221 atcgcattgc gtggctgatt ggggtggaaa atgtgccgga atccaatatt ctggctgtta
    38281 cctttaccaa taaagcggcg gcggaaatgc gacatcggat tgagtacact ctttcccaat
    38341 cgggcgataa tcgattgttc ggaatgtggg ttggtacttt ccacagcatt gccaaccgct
    38401 tacttcgttc ccactattta gatgcggatt taccgcaaga tttccaaatt atggataccg
    38461 aagatcagca acgtttactc aaacgtttac tgaaactgca taatattgat gagaaacatt
    38521 ttccgccaaa acacgttgcg tggtatatca acgcccaaaa agataaaggc aaacgggcaa
    38581 aagatattga tcactataac gatccgaatg aaaaaaaatt ggtggagatt taccaaatct
    38641 atcaagatgc ctgcgaccga gccggtttgt tagattttgc cgagctgttg attcgggctt
    38701 atgaactatt caaaaccaaa tcaaccattt tgcaacgtta tcaacaacgt tttcagcaaa
    38761 ttttgattga tgagtttcaa gacaccaaca atattcagta cgacttaatt cgtttgcttg
    38821 ccggcgatgt cggcaaagtg atgattgtcg gcgatgatga tcagtctatt tatggttggc
    38881 gtggggcaca ggttgagaat atccaacgtt tcttaaatga ttatgacaac gctcaaacca
    38941 ttcgcttaga gcaaaattac cgctctaccg gcaacatttt aagctgtgcc aatgaattaa
    39001 ttgccaataa cgataaccgc ttaggcaaaa atttatggac agatagcggt gatggcgagc
    39061 ctgtggatat ttactgtgcc ttcaacgaac ttgatgaagc tcgctttgtt gcctcgcaaa
    39121 tcaagcaatg gaaagaagac gagggtgatt tggcggaatg tgcggtgttg tatcgcagca
    39181 acagccaatc tcgtgtgatt gaggaagctt taattcaagc gaatattcct tatcgcattt
    39241 acggtggaat gcgattcttt gaacgccaag aaattaaaga tgctctggct tatttacgct
    39301 taatcgccaa tcgtcaagac gatgcggcgt ttgaacgggt gatcaacacg ccaaccagag
    39361 gcattggcga tcgtacctta gacacactcc gtcaaattac ccgtggtcgc caaattacgc
    39421 tgtggcaagc aattcaagtg gcggtgcaag aagaacaact ttcagggcgt gccacttctt
    39481 ccttattgcg ttttgtagag ctgattaatg ctcttgatca agaaaccgag cagatgtcgc
    39541 ttgccgaaca aaccgatttt gtgattaaaa aatcgggttt atatgaaatg tataagcaag
    39601 aaaaaggcga aaagggcgaa gttcggattg aaaacttaga agagttagtt accgcaacta
    39661 agcagtttgt gaaacctgac gaagcggaag agctgaccga tcttaccgca tttttaaccc
    39721 acgcctcact tgaggctgga gaagcccaag cctcgcaaca cgaagattat gtcgaactga
    39781 tgacgctaca ttccgccaaa ggattggagt ttccccgtgt gtttattgtg ggcgtggaag
    39841 aagggatttt cccaagcgga atgagctttg atgaaggcag gttgcaagaa gaacgccgtt
    39901 tggcttatgt cggcataacc cgagctaaga aaaaactgac gattagctat gccgaatcaa
    39961 gacggcttta tggtaaagaa gaacgccacg ttccgtctcg ctttattgct gaattacctg
    40021 aagagcatat tcgagaagtg agattgagag gctcaattaa ccgagcggca agttttaacc
    40081 aagctaattc atttgcaaaa aatccgacaa aaacgaccgc ttccttttta aacgatgata
    40141 cttggaaaat ggggcagaaa gtgaaccacg ctaaatttgg acaaggcacg attattaatg
    40201 tggagggctc aggtgaagcc acccgtttgc aagttgcctt tgtcggcaac ggcattaaat
    40261 ggctgattgc caaaatggca aatttagaga ggatttaggc gaataaaaag ttttttgcag
    40321 gaatatagat ggcgagatat attgcccctt aaccttaaac taagggtaaa taaaattaaa
    40381 taatgcaagc tagatataat gtaaagtatc agaaataaga tattaattta agataaagaa
    40441 tagatggctg gggtactagg attcgaacct agggatgccg agatcaaaac ccggtgcctt
    40501 accgcttggc gataccccaa aagaaaaaac taacttacgt tagcttatat agatttgaat
    40561 gtttgaaatt aagtggctgg ggtactagga ttcgaaccta gggatgccga gatcaaaacc
    40621 cggtgcctta ccgcttggcg ataccccaac actaattttt gttaatcgat aaatggtgcg
    40681 ggacgaggga cttgaaccca cacacctcgc ggcgccagaa cctaaatctg gtgcgtctac
    40741 caatttcgcc agtcccgcaa tggtggctac agcgagattc gaacttgcga ccccaacatt
    40801 atgagtgttg tgctctaacc agctgagcta tgtagccatt tgcgttcacc atatcggtca
    40861 acggagtgca ttatgctgat tttagggctt tccgtcaaat acttttttta aaaaatttga
    40921 atttttgatt cgcttgtata gttattcgtc gttttggctt aaatctagca ttttttgttt
    40981 ataaaataat agcttatatt ctgctatttc accgtttgca tttctaattt taattgagcc
    41041 agtacaggct caacatcggg gaattgccct tcccacaaag aaaacgcaaa cgctgcctgt
    41101 ccaaccaaca ttcctaatcc gtcttggcac gctgttgctc cacaacgcct tgcataattg
    41161 agaaaaggag tttgcatatt gggggcatat tgcatatcgt aaatattcgc cgatgctata
    41221 acagattcag gcaattcaac atatttgcct tgcagaccga gagaggtggc attgacgatt
    41281 aaattaaaca gttggtttgc caaatcgctt aaacaagcgg tctgaatttt gccaaatttt
    41341 gcaaattttt cggctaaaac gaccgctttc tcctgcgtgc ggttatatag agtaatctct
    41401 aaccccgctt taagtaacgg aagtaatacg cctttggttg ctccacctgc tccaagaatt
    41461 aaggttttat gccctttttg taaccaacct agccgttcca aatcagaaac taaaccggcc
    41521 ccgtcggtat tatcggcata taaccgtcca tcttctaagc gtttaagcgt attacacgcc
    41581 tgtgccaaca aagcggtttc gctaaattca tctgccaatt taaaggctct ttctttaaaa
    41641 ggggcggtga tgttgcaacc ttttgctcca gaagcgaaaa aatcggctaa ttgttgctca
    41701 aaagccgttt catctcctaa gttagcgata tattcaatgt tgcgttgggt ttgtaaagca
    41761 aagagttgat gaatacgggg ggatttactt tgagcaatcg ggttgcccca cacggcatat
    41821 cggttcattt cggcttatcc ttgtctaaaa atctgttggc tgaaaatatc tcgaatttcg
    41881 gaagggtttt ctctacctag cgtgtctgcg tgtaatacag gaaaatcttg accaaactga
    41941 tcgatgactt cttttgcatt gcggcaaggt ggttctccgc tcaaattggc actggtagag
    42001 gtcaacgcaa cgccgcttgc ctcacacaac tgaaccaccg ccggcacatt acataaacgt
    42061 acggcaatag aatcaaattt gccggtgagg taacttggca cagtattttt aaccggtacg
    42121 atccaagtaa ttgcctgctc gccaatatgg cttaggcgtt gccaagcctg ttccgtcatt
    42181 cgagcttcat caatataggg aagaagatac tttaaatcag aagccagtaa aattagccct
    42241 ttttcctcag ggcgttgttt taatacgagt aatttcctta ccgctttttc actttgtgga
    42301 ttgcagccta agccaaaaac cgcttcggtt gggtaagcta caacttcttc ttgctttaat
    42361 tgattaacta tttctaaaaa attattcatt cggttttaat tctgtttggt gctggcaact
    42421 tttgttagca caaatggtga attgcttgcc cttaattttt ttgattgtag cgagttcaca
    42481 accacatttt tcacacaagt ggttaatcgg tcgagttgct aatgtaaatt tgcaattagg
    42541 gaagcctata caaccataaa atattttgcc tgaacgccct tttctagcaa tcagtttatg
    42601 ttgcttgcat tcagggcaat caaactcttc ttcaacctca gcttcttcgt gtacgatgaa
    42661 gtggcattca gggtaatggc tacagccgat aaaaatacca taattgcctt gttttaattg
    42721 gaggtttccg ccacattcag ggcaaagttc atctaattgc ttaataatat gaccggcttg
    42781 atgtaatggc ttaatataat cacattcagg gtaagctgaa caacccaaaa ataacccttt
    42841 tttgccctgc ttaatttgca acggcgagcc acattgtggg caagtttcgg tctgtttggt
    42901 tgatttgaaa agtaccatat tgatttgcta aataagcgta aattcaatca caaaaaacgc
    42961 attgtcattc ggataaatct cccgaataac ttgtttcaat tcggctaagc tcatattttc
    43021 ttgtttagcg tgttgctcgt ttaattcatc aaacactata ggtgttaccg atagcacttt
    43081 aatattggcg aaaaatctat cggtttcatt agtaaaaacc gctaattctt ggtttggttg
    43141 aaaataagat tctgacttgt ctcgaatcgt aattgttttt ttaccgctta aaatatcggc
    43201 ttcaaatcgg ctgaaaaagg taattttctc tctattcata ttcatcgcta ctgtaatcca
    43261 gagccattaa atgactaaaa tgatcgttat aaaaaccacg gctaaccata aatcgccaca
    43321 ttttctgctt tgctttcaga tcccatacaa gcggtcgttt tttctcaaaa atttgctcag
    43381 ctaattcaaa ccaatccact tcggcgtttt ctaattcttg gttaattaac caatctttta
    43441 cccctttaag ttttagctct tgctttaaac ggcgaggccc aatgccttgc attgaacgat
    43501 aacggataaa agtggtacaa aagcgttcat cactttgcca ctgattcgct tgagcttttt
    43561 caatcgctgc atcaatctcc gtgagttcat actctttttg ctttagtttt tgccgaagct
    43621 cttgctcgct gtaatcacgc tttgaaagta aatatagcag ataattaacc gctgtgtatt
    43681 tatttgtttt agtagtagat gttgtcattt atcggttata aataaaaatg cgattttcac
    43741 gttgagcgaa aaccgcataa atttaattag agttcttctt cactaaattc atcatctgca
    43801 ttgtctgcat tttcagaatc tggagtagcc gtaaaagttg ggtttgaaat gagtagattt
    43861 cgaatatctt gctcaatttt actcgccact tcagggtgtt cttttaacca tttgatggca
    43921 ttatttttac cctgaccgat tttctcccct tcataagaga accaagcccc tgctttatta
    43981 atgaagccgt gagattccgc taaaatgagt aattcattca tacgggcaat gccctcaccg
    44041 tacataatat caaattggac ttcacggaac ggtggagcaa ccttgttttt cacaattttc
    44101 acttttgttt cgctaccgat aacctcatca ccgtctttta ctacgcctga acgacgaata
    44161 tctaaacgta ctgaagcata gaatttaagg gcattaccac cagttgtggt ttcagggtta
    44221 ccgaacatta ctccaatttt catacgaatt tggttaatga aaatcaccaa gcagttggtc
    44281 gctttgatat ttgcggttaa tttacgcaat gcttgtgaca ttaaacgagc ttgtaagccc
    44341 atatgggaat cacccatatc gccttcaatc tctgcttttg gtgtaagtgc cgcaacagag
    44401 tccacaataa ttacatcaac cgcaccggaa cgaactaacg catcacaaat ttctaatgct
    44461 tgttcgccat tatccggttg tgaaattaat aatccatctg tatctacccc taacttacgg
    44521 gcgtaaatag gatcaagtgc gtgttcggca tcaataaagg cacaagtttt accatttttt
    44581 tgggcttgag ccaccacaga taaagttaat gtagttttac ctgaagactc cggtccataa
    44641 atttccacaa tacgacccat tggtaaacca ccaatgccta atgctgagtc taaaccaaga
    44701 gaaccggttg aaactgcttc aatatcaagg gcttgagtat cgcctaactt cataattgag
    44761 cctttgccaa attgcttttc aatttgagct aatgcggctg ccaacgcttt ttctttttgt
    44821 tccggatcaa tctgtttaac cggagtgttc ttttgacttt ttttctctgc catattctat
    44881 cctttgtaaa cttttatcta aaagcaaccg cttgttgctg caaaataact gtatattatt
    44941 atagtattta cttatccagt tgcaagcatt tttttaggat tttcgatcaa gatcacaaaa
    45001 ataaatttgc gagcaaggca atgcccttgg ttcctcaaag ggattaagag cattaatcaa
    45061 cataattttt tcatattgat gcaaatcttg cttggctatt ttggctaaaa cgactttccc
    45121 ttcatcaagc aatttactaa gctgagtgcc tttgagcaaa taatgttgcg ggctatacca
    45181 gattccttgt tttaggaaca gtaaattgcc gatggagcaa tcactgacgt agccattatt
    45241 gagaataatc acctcttcat tttgtaaaat agggagcaaa tcaaaccgct tgcggtcgct
    45301 aaatttgagg tgataatccc aattttccac ctcaaccaag cgaaaacgtt ctactttttt
    45361 gggcgaatag ataaaaaatt tcagctcaaa ttctgttgcg ttgtaatcta ttcggcaacg
    45421 tactttgccc cgttgagaag cctctggcac aactaaaata tcagcaaggc taaaacgagg
    45481 ttcacagcca aaatattctt gtgttgcttg ctcaaaacgt tgttgatgat actgtaagtt
    45541 ctgaactttg ccgtcaataa tcgaaagggt ttcaaatagc ggaaatcggc acatagactt
    45601 tctctaacag ttcattatat tcatcagtta acacactttg tgaggtaatg ccaccaccac
    45661 tatgaaacac aagcccgttt gaggtttgag caatgtaacg aattgccacc gcactttcta
    45721 agctctcgcc atcaaaataa ccaaaaatac cggtgtagta accccgtttt cctaattctg
    45781 cttgctgaat aatcgaaacc gttttctctt tcggtgcacc gctaatggag ccggcaggta
    45841 gtaattttgc caatatcgaa ccgatatttt cttgccaatt ttcacctaat tcgccacaga
    45901 tctcggaact tgtctgcaaa attgcccctc gatgggtttc tactttttcc acataacggt
    45961 atttagtaac ttcaatattt ttcgccacca acgccaagtc atttcggatc aaatccacaa
    46021 tggtattatg ttcggtaaac tctttttccg aagccattaa tttctgctcg gcattctcct
    46081 catttgcatt aattgtgcct ttcatcgggt aggaaaaaat gcggttattt tcaattcgca
    46141 caaagcattc aggggaaaaa cagacgaacc tgccttgcaa ataacattta tattttgctt
    46201 tgctggcggc aaaaatttcc gccaatgaat aattggtttc aatggcagtc gggtagctca
    46261 aattcagcaa gtagctattg ccggcttgaa tttccttttt cactaattca aacccggttt
    46321 ggtattcgct aaacggaatc ggatttgccg ataattcaaa agctttagtc ggtttttcaa
    46381 ccggcttttc gagattcgat ttgcaaaaaa attcaaaaaa aagaccgctt gcgtgagcct
    46441 gttctagcgg aaaaataagt ggcttttgct gctcaaaatc aattaaaaag aaaaaagggc
    46501 gttttgcttg cccgtagtga ttggcttggt gaataaaatc ttgcatagaa aacacaatgg
    46561 ctgaaattgg ctgaatcatc aaaagctctg ttataatgca aagcaataga tttgaaaagc
    46621 attttttaaa aaaggtttta ccgatgaaca acaccattca ttttcaagaa attgggatct
    46681 acatcgccct tgccgtttgt gtgctggtag gcgtttatct ctttttcttg aaatcccgtt
    46741 atcagcgaaa tgcctacgaa ttaagccaag atcttggcaa aatcaccgaa aattttgaac
    46801 aaattcagca aaaatatgag gtgttggctc aagaaaaaaa tcagcttgag cagtgggcaa
    46861 ttcaacaaca gaccaaatat gaagcagtga gtgaacgttt aaacgagcga gatgggcaat
    46921 tgcaacgatt ccaacaaaaa atcgaacttg ccgaacagca agaaaaccag ctggaacgct
    46981 atattaatga gctaaaagaa cgtgtgggtt cttcccaagc aaaggcagaa agtttggaag
    47041 aacagctaca atttagccaa agttcactct caactaaaga gcgggaaaat caagcgttat
    47101 ttagccgttt aaacgaggta caaaacgagc tgaccgaact gcgtactacg ctttcggaaa
    47161 aacaggcaaa ttttgaagca caacaacgca attttattga ggtaaaacaa cagcttaatg
    47221 tggaatttca acatttggca caacaaattc ttgacgaaaa aagcaagcgt tttagcgaaa
    47281 ccaatcaaag ctcgttagaa gctctactga agccgttcaa agagcaaatt gaaggctttc
    47341 aaaaacgggt gaatgaggta catagtgagt cgctcaaagg ctcggcaaat ttagaggctg
    47401 aaattaagcg ggtgctacaa attggcgttt caatgtcgga agaggcacaa aatctggcaa
    47461 cggcattaaa aggcaataat aaaattgccg gcaactgggg tgaagtacag cttgaaagtg
    47521 ccttacaatc tgccggttta ctcgcaggcg agcattacca agcccaagag agttttcgtg
    47581 atgaagaggg gcgacgtttt gcccccgatt ttgtggtgca tttgccggat caaaaacatc
    47641 tgattattga cagtaaagtc tcgttagtag cgtacgatca agcggtcaga tcagaagaaa
    47701 attttgcaat ttctcaagcc ttagacgaac attgccgttc gctacgcaat catattgagg
    47761 gattgtctaa gaagaactac agtgcattat taggcattaa aagcccggat tttgtactga
    47821 tgtttattcc gatagaaccg gcttatattg aagcgatgaa gcacgatccg caactgttta
    47881 actttggcta tgaacgcaat gtgattttgg tttctcacac caccttaatg ccgattttac
    47941 gcacggtcgc caatttatgg cggattgaac gaggcaacag cgaggctcgt gaaatcagcg
    48001 aaaaagccgg cgaaatttac aaccaagtgt gtgcggttgc cgatcggtta gctaaattag
    48061 gtaacacgct aaataccgca aataatcaat acaatcaaac cgttacctcg ttagtgggac
    48121 ggcaaggctt actgggcaaa gtggaacgat tccaacatct ttcaagcaaa gcaagccaaa
    48181 ttgtgccgca ggttgagctg attgaaaatg aggtggatac tattaaattg gaaatggtat
    48241 cgagagaatt attggaagaa tagtcaaatc tctttattcc ctacaaaaat atggggtgat
    48301 ttgaaatact aaagaaaata ataaaatgct aaaaaaacta ttaatcatcg acaaccacga
    48361 ctcttttacc ttcaacttgg tggatttact ccgtaagctt gatgtgccgg cgaaagtggt
    48421 gctggttgaa gagcttgacc tcaatgaggt ggagcatttt agccatattt tgatttcgcc
    48481 cggccctgat gtgccgagag cctatctaca aacctttgcg atgttggagc gttatcatca
    48541 aaccaaatca attctaggcg tttgcttggg gcatcaaacg atttgcgagt tttttggcgg
    48601 caagttgtat aacctaaacg atgttagaca cgggcagcaa cgcacgctca ctcaaattga
    48661 gcctaacccc atatttaaag gtttgcctaa gcagttgaaa gttgggttgt atcactcttg
    48721 gggcattttg caaaattcgt tgcaaaatac accgcttgta ccaaccgcag tttgcgatca
    48781 aaatgtgtta atggcgttta aacaccaaca tttgccgatt tttggcgtgc agtttcaccc
    48841 ggaatctttt atcactgaat ttggtcaaca aatgttggca aattggcttg agattacaca
    48901 agatttttag cgtgaaatcc gctataatcc gtcagttatt taccctttta aaatctgtag
    48961 gggcgaaaaa tttttcgcct tacttaatat gcaagagata atttaaatga aagattcgat
    49021 tatcaataaa ttagaaagtt taagtgaacg ccacgaagaa ctacaggcgt tacttggcga
    49081 tgcctcaatt atttccgatc aagacaaatt ccgtgcttat tccaaagaat attcgcaatt
    49141 agaagaagtg gttggcacct ttaaccgctg gaaaaagctg aacaatgata ttgaagaggc
    49201 tcaattatta ctcgatgatc ctgatatgaa ggatatggca gcggaagaaa ttgcggaaaa
    49261 taaagccgaa atcgagaatc ttgagcaaca tttacaaatt ttattattgc cgaaagatcc
    49321 gaatgatgaa tacaacgcct tccttgaaat ccgtgccgga acgggcggtg acgaggctgg
    49381 tatttttgcc ggtgatttat accgtatgta cagccgttat tgcgaaagta aacgctggcg
    49441 aattgaagaa atgtcggcaa atgagagtga acaaggcgga tataaagaga tcatcgttaa
    49501 aatttcaggc gaaggcgttt acggtcagct taaatttgaa tcgggcggac accgtgtaca
    49561 acgtgtaccg aaaaccgaat cacaagggcg tattcacacc tctgcttgca cggtagcggt
    49621 aatgccggag ttgccggaaa gcgagatgcc ggaaatcaat ccatctgatc tgcgaattga
    49681 cacctaccgc tcatcgggtg caggcggtca gcacgttaat accaccgact ctgcagtgcg
    49741 gattactcat atcccgaccg ggattgtggt cgagtgtcaa gatgagcgtt cacagcacaa
    49801 aaataaagcc aaagcgctgg cagtgttagc ctctcggatt gtacaagttg agcaagaacg
    49861 ccaagccgca gagcaagcgg atacccgccg taacttactt ggctcaggcg accgttcgga
    49921 taaaatccgc acctacaact acccgcaagg gcgtgtgact gatcaccgta ttaacctcac
    49981 ggtttatcgt ttagacgaag tgatgaacgg caaaattgat gaattgatac aaccgattat
    50041 cactgaatat caagcggatc agttagcggc gttgtcggag caaaattaat tttattgtaa
    50101 gggtacactg tgtgtgcctg ccaaatataa atttgcaaaa ttttgactat ttttgatcgc
    50161 ttgtagtttc taaaaagttt aaaaatttga ggaaaacaaa atgagaaagt taggtttatt
    50221 agcagtattt actttagtat tatcagcctg ttcaaattca acaaaaattg aacaaaccat
    50281 tctttccaag caggatatcg taggctcttg ggtatgcgct attaaatacg atgatctaaa
    50341 agtaagcact ttagattttt ctgaatttaa agctaatggc gaaatgacga atttcggtga
    50401 tatttctgat gagaattttg agcccattaa atttacttat gtaacaggag ataaagggaa
    50461 gtgggactta aaaggaaatc gattagtgat tgattatgat ctttctgaac gtaaagtaaa
    50521 aagaacaaca ccaaaaaaat ttttagagtt cttgaaacag gaagagcgta aagcctcaaa
    50581 aacagctaaa aaactcctta aatatgaaca agggattttc aatattctga gtgatcagaa
    50641 aaatactgaa aacagtaaaa taatattaga tattttgaag tttggtcatt caagaatggc
    50701 gattcagcaa aatatgggag acaaagttta ttcaggtggt tgtgtaacag ctgataaagc
    50761 agaagagtat ttagatatcc taaaaaatag aaattctaaa taatggatta ccgagagtgg
    50821 ctcacttttg ctgagcaaaa attacttgaa aatagcgggg cagatccata cctaaacgca
    50881 aaatttgatg caaatttgct attgcaagca gtcacaaaac gttcaaaatc ggccattttt
    50941 gctttttcgg aaaccctact ttctgaaagc gagcttgcag aattagcgga aaaattagct
    51001 cggcgtgcca aaggtgaacc aatggcatat attttaggct atcgggaatt ttggtcgttg
    51061 ccgttaaagg tttcaccggc aacgctgatt cctcgccctg atacggaacg actggttgag
    51121 ttggcgttgg aatatgctaa caagcggtta ggaaatcaga aaatgttgca aattctggat
    51181 ctcggtaccg gcacgggggc gattgcgttg gcactggcga gtgaattagg cgagaaagcc
    51241 caaattatcg gggtggattt ccaaaatgag gcggtgcaac tggcagaaga aaatcgccga
    51301 aacttaggct taaatcaagt tacattttta caaagcgatt ggttcagttc gcttgcaaat
    51361 caacaatttg atttaattgt ctcaaatccg ccttatattg atgaatcgga cgaaaatttg
    51421 acggtgggag acgtacgttt tgaaccttta accgcattag ttgccgataa taaggggttg
    51481 agcgatttgc aaaaaatcat cgaaaatgca ccgcttcact taaaaccgca aggggcgtta
    51541 atgctggaac acggctggaa acaaggtaac ttggttcgag atttatttga tttacacttg
    51601 tgggaacagg tagaaactgc tcaagattat gccggcaatg accgagtaac caaagcgatt
    51661 ttaaaataag gcagaaaatg gacgatatta ttgaagaatt gttaaaagag ttggaagatc
    51721 cgaaagtaac catcagcgaa accgagctga aaaagtttct ttatcgtgaa attttacgat
    51781 taactacgct cattgataag aatgtaagtg agcaacaagt gtttggtcag atgtctgcgt
    51841 tggtgaaaaa agctcgctat aacgtgaatg gcgaaggcga tgaagagaga attaaccaat
    51901 tacttcattt agtctatcgt gaatggggct ttcattgcta ctatgaagac tatttccaca
    51961 ccgaaaattt gttgctaaat caagtaattc gcaaacggcg tggaatgccg gtttcacttg
    52021 gggcggtggc gctttattta tccgccgtat tggatttgcc gctttacccg gttaattttc
    52081 cgactcagct tgtgttacgg gctgaaatac accagccgaa cggggcaacc aaagtgcgtt
    52141 ttatcaaccc gtggaacggt gaatttctca ccatcgaaat gctgaataaa tggctggaag
    52201 gagaaatggg ctttggcgta gaagttaccc ctgatttgtt aagacgagca gaaccacaag
    52261 agttgctgga aagggtcgaa actgtgttta aaatggcact tacccgtgaa ggcaaatatg
    52321 aagaaacctt acgcttgatt gaataccgct tggcatttag cccggaagat ccgtacgaaa
    52381 tcagagaccg aggaatggta ctggcaagta tggattgcta ccaagcagca ttagaagata
    52441 ttaattattt cattgaccaa tgccctgaag atccttctgc cgaaatttta aaaatggaag
    52501 tgaaaggact gcaacaaaaa agccaagaaa gtgtggtgca ttaagctgag aagtagcccc
    52561 aaaggggctg agctatgatt aacctagtac ggctctgccg tgctaggcag aaaataagaa
    52621 acaaggactc cctatgacag ataaaattgt aaaagttggc aatattgaag tggctaatca
    52681 caaaccgttt acgctttttg gtggtatgaa tgtgctggaa agccgtgata tggcacttag
    52741 agtatgtgag caatatgttg aagtcacgca aaaactaaat gtgccttatg tgttcaaagc
    52801 ctcatttgac aaagcaaacc gttcttcaat ccactcttac cgtggaccgg gaatggaaga
    52861 aggcttaaag attttccaag aattaaaaca aacctttggc gtgaacatca ttaccgatgt
    52921 tcacgaaatt taccaatgta aacctgtggc agaagtagtt gatgtgattc aactaccagc
    52981 atttttggct cgccaaaccg atttagtgga agcaatggct cgtaccggtg cggttattaa
    53041 cgttaaaaag ccgcaatttt taagcccggg tcaaatgggg aatatcgtag aaaagattgc
    53101 tgaatgcggc aacgatcaag tgattttgtg cgaccgtggc actaactttg gctacgataa
    53161 tctagttgtg gatatgcttg gtttcggcat tatgaaaaaa gtgtcgaaag gttgcccggt
    53221 gatttttgat gtgactcact cattacaatg ccgtgatcca ttcggtgcgg cttccggtgg
    53281 tcgcagagat caagtaaccg aactggcacg ctccggtatg gcgattggtt tagccggctt
    53341 gttcttagaa gctcacccgg atccaaactc ggcgaaatgt gatggtccat ctgcattacc
    53401 gctgtcaaaa ttagaagcct ttgtttcaca gatgaaagcg attgatgagt tggttaagag
    53461 ctttgaagaa attgatactt ccaaataaac tttctccgca ttaaatcctt tcctactttc
    53521 tttgcttcgc caaagaaagt aggcaaagaa aggcgaccct acttcgctac ttttcttcgc
    53581 ttagtgtgaa tgggcttaac gaaaaattta cattcgcttc gctcagagta aattttccta
    53641 cccattaaaa cacgctcagg tagctcagaa gggagttaga aaggtaaaag acagaatagg
    53701 agaaaaaaat gctaaatatt gtttttcttg accgtaccgg tattcctgcc actcacgata
    53761 ttcctcgtcc gaattttcca catacttgga cggaatatgc caccacctct gccgatcaaa
    53821 cttttgagcg ggtaaaagaa gcggatatta tcattaccag caaagtgtta ctccagcgtg
    53881 acttgctggc taaattacca aagctgaaat taattgcgat cacagcaacc ggcacgaaca
    53941 atgtggactt agcggcagca aaagagttag ggattgcagt gaaaaatgta accggctatt
    54001 ccagcgttac cgtgccggaa cacgtcattg gtatgattta ctccctgaaa catcgtttag
    54061 ccgattatca ccgtgattta attacgtctg accgctgggc aacttgcggc caattttgct
    54121 atgtagatta cccgatccac gatgtgcgag gctcaacctt aggcattttc ggtaaaggga
    54181 acatcggggc tgaagtggca cggctggcac aagcggtcgg aatgcaggtt atttttgcag
    54241 aacatcaagg cgcaactgaa attcgagaag gctacaccgc ctttgaagag gtattcaaac
    54301 aagcagatat tatttcacta cactgccctt taaccgagca gactcaaaac ctgattaatg
    54361 cagaaacctt agccttaatg aagccgaccg cttatattat caatacgggt cgaggcccac
    54421 tgattgatga acaggcattg cttgaggcat tagagcaagg caagattgcc ggcgctgcgt
    54481 tagatgtttt agtgaaagag ccgcctgaaa aagacaaccc tttaatgtta gccgcaaaac
    54541 gtttaccgaa tttactgatt accccacacg ttgcgtgggc aagcgattct gcggttacaa
    54601 cactagtgaa taaagtgaca caaaatatgg aagagtttgt agcaacgggt atgtagcttg
    54661 aagtaggggc tggtcctgtg ccagccctaa tattaataga atgagagggc gggtataaaa
    54721 cccgccccta ccaatatcat tttagaaaaa cagatgaatt taaccccttt ttttattgct
    54781 tttcgctatt ttagatcgaa aagtgctgac cgtttcgggc ggttagtcac caatcttgcc
    54841 agccttggca ttgtgctggg cgtaatggca ttggtgattg tgctgtcggt tatgaacggt
    54901 ttggaaaata tgcagaaaac caatctgcta tccaccttgc cgcacgctat catcacccct
    54961 caagaggggc attttgccaa aggtgaaaaa ctggctctac ctgaatttgc caagcaaagt
    55021 gtggtgatta accgtgcgaa tgtgattatc caaagttcac aaggcattaa cgccggtcag
    55081 ttaatcggcg tggaaaatgc cacagacgat ccgttgttag tggcggaaaa tatccccgca
    55141 ttattgccta gcggtgaatt taaggtagta attggctctc gtttagccaa taaattgaac
    55201 gttacggtag gcgataagct ccgcttaatg atcactgaaa acagccaata tacgccactt
    55261 gggcgagtgc cggtacagcg tttgtttgaa atttcgggcg tttatcaatc taatcgtgaa
    55321 gcgagcgaat ttaccctctt tgctaattta gctgatgtcg ggcgtttgct tcgcatcagc
    55381 gaagatcaag tacaaggcgt gcgtttatat ttgcaagatc ctttccaagt taccgaactt
    55441 agccaatatt tcgatgcaga acgctaccaa atcagtgatt ggcgtgagca aaaaggcgaa
    55501 tttttccaag cggtgaaaat ggaaaaaaat atgatgggct tactcatcag cctgattatt
    55561 gtggttgcaa tttccaacat tgtgacttca ctcagtttga tggtggtaga caaacagggc
    55621 gaaattgcaa ttttacaaac ccaaggctta accaaaaaac aagtgatgca gatttttgtg
    55681 ttccaagggg cgattgtcgg agtaattggc tcgattattg gtggtttgct cggcgtattg
    55741 atcgcctaca atttagatca gattatcctg ctattaaacc cgagcatcca tctgcctacc
    55801 ctaatttcag gcacgcaaat tgcggtcatt gtaggggtgt cgatgttgct ttcactcctt
    55861 tgcacgcttt acccggcata ccgagcctca aaaattgagc cggcacaggc attgagatat
    55921 gaataataag aacggaaata aaatgacaga attacttcgc tgtgaacata tcagcaaatt
    55981 ttacgatgag ggcgaacaga aagttcaggt gctaaaagag gtgtcatttt cgatgaacga
    56041 aggtgagctg gtagcgattg tcggtagttc aggttccggc aaaagcaccc tattgcacac
    56101 attaggcggg ctagatcagc caagcaccgg tgaagtctgg attcgagggc aatctctgca
    56161 acagctcagt tcagacaaat tagcgttatt gcgtaaccaa aacttaggct ttgtgtacca
    56221 atttcatcac ttaatggcag actttaccgc ccttgaaaat gtgatgatgc cgatgctgat
    56281 cggcaaacag aacaaaactg aagctgccaa tcgtgccgag aaaatgctac aagcggtcgg
    56341 attagctcat cgaatgacac atcgcccatc agcactttca ggcggcgaac gccaacgggt
    56401 ggcgattgcc cgagcgttgg ttaataatcc ggctctggta cttgccgatg aaccgaccgg
    56461 taatttagac caaaaaacca ctgaaagcat ttttgagctg attcaacagc tcaaccaaga
    56521 gcagaacatt gcgtttttat tagtaacgca tgatttaaat ctagcgaata aactttcacg
    56581 tagtcttgta atgcgagatg gcgtgttaag cgaggggagc ttatgagtac gccgtttttc
    56641 attagctggc gttaccaacg gggcaaacag aaaaacaaac tggtttcact gattgctctc
    56701 ttttccaaca tcggtattgc ccttggggtt gcggtattaa ttatcgggct aagtgcaatg
    56761 aacggctttg agcgagagct gaataatcgt gtgctttcgg ttgtgccaca cgctgaactg
    56821 gtttcgtttc aaggcaatca gcctcaacca atttttgata gcaaaaaatt agaggcattg
    56881 ataaaaaaat cgcaaaatgt gaccgctagt tcgccttttg tcagctttac cgccttaatt
    56941 gaaaatgggg ctcagcttaa aatcgcccag gtgcgagggg ttgatcctaa aaagcaagat
    57001 caggccagcc aattaagcca atttattcca cccgagcaat ggcaagcgtt caatcagcaa
    57061 tttcaagccg atgaagatgg cttgatttta ggagccggta ttgccaaagc gttagaagtt
    57121 gaggcaggag acgaagttac gcttttaatt cctcagccaa cagaagacgg caaattagcc
    57181 cagcccttgc gttttaacct aaacgtaacc ggcattttgc ggctagatgg gcagctggat
    57241 cacagttatg ccttaattcc actgaataaa gctcaagagc tgatggaata ccaagcgaat
    57301 gaagtgtcag ggcttgaaat cagccttgcc aatccgtttg atgtacaaca gcttgaaatg
    57361 ccacaactta acggctatac ccaaccgctt tatttgaata cgtggattga taaattcggt
    57421 tatatgtata acgatattca actgattcgc accgtgatgt acattgcaat ggttctcgtg
    57481 attggcgttg cctgttttaa tattatttcc accttgatta tggcagtgaa agacaaacaa
    57541 ggcgatatcg cgattttacg gacactgggg gcaaataacg gttttatccg ccgcattttc
    57601 ttatggtatg gcttaatttc aggtatgaaa ggggcgttat tcgggctgat tttaggtgta
    57661 attttatcgc taaatttaac cgctattatc aaagcgattg aagcattctt tgaggtgaaa
    57721 ttgctttcag acggggttta ttttgttgat ttcttaccaa gtgaattaca ttggcaagat
    57781 gtcgtctatg tattggcagc cacgattatc ctaagccttt ttgccagctt ataccctgcc
    57841 aaccgagccg ctaaattaga accggcaaaa gtgttaagtg ggcattaatc attttcctat
    57901 tctaataaaa aagcagctac ccctcgatta tgtcagaaag gtagctgctt ttatttatgt
    57961 tattactctt cgcctaataa tttcaactca cgagccttaa tttggttttt tagaatttca
    58021 actaagtctg aaacgaggta agtacctaag tctgcacctt tacgggtacg cacagacact
    58081 ttaccttcgg cgatttcttt atcaccgcaa accaacatat aaggcacacg gcgtaaggtg
    58141 tgctcacgca ctttgaagcc gactttttcg ttgcgtaagt ctgctttcac acgaatgcct
    58201 gcatcagaaa gctgtttcac cacttgttgc acataatctg cttggctgtc ggtaatgttc
    58261 attaccactg cttgggttgg ggcaagccaa gttgggaaga aaccggcata ttcttcggtg
    58321 ataatcccga tgaaacgttc gatcgagcct aaaatcgcac ggtgaatcat taccggtgtg
    58381 cggcgaccat tatcttctgc tacataagag gctgctaaac gacccggtag agcgaagtca
    58441 agttgcactg tgccacattg ccattcacgt cctaagcaat cacgcaatgc aaactcaatt
    58501 ttcggcccgt agaatgcacc ttcgccttct tgaatttcat attcaagtcc gttgttgcgt
    58561 aaggcgttgg ctaagccttt ttctgctctg tcccacattg catcatcacc gatacggttt
    58621 tccggacggg ttgaaagttt tactgcaatg ttagtaaaac cgaaggtgct gtaaatgtcg
    58681 tacaccattt tgatacagct gattacttct gactcgattt gttcttcagt acagaaaata
    58741 tgggcatcat cttgggtaaa accacgcaca cgcattaagc cgtgtaatga accggacggc
    58801 tcgttacggt gacacgaacc aaattccgcc atacggatcg gtaaatcacg gtaagatttc
    58861 aagccttgat taaagatttg aacgtgaccc gggcagttca tcggcttaat tgcatattca
    58921 cggttttccg attgggtagt gaacattaaa tctgcgtagt tttgccagtg tccggttttt
    58981 tcccaaagca cacgatccat cataaacgga ccttttactt cttggtaatc gtactctttc
    59041 aatttggtac gcacaaaagt ttcaagttca cggaaaatcg tccagccgtc attgtgccag
    59101 aacaccatac ccggtgcttc ttcttgcata tggtataaat ctaacgcttt accgatacgg
    59161 cggtggtcac gttttgccgc ttcttccaaa cgttgtaaat attcggctaa ttgtttttta
    59221 tcagcccaag ctgtaccgta aatacgttgt agcattttat ttttgctgtc gccacgccag
    59281 taagcacccg ctactttttg taatttgaag tggtggcaga aacgcatatt tggtacgtgt
    59341 ggaccacggc acatatcaat atattcttcg tggtgatata acgcaggcgt tgccgtacgt
    59401 tcaatatttt catctaaaat cgccattttg tatggctcac cacgtttctc aaaggtatct
    59461 cgagcttctt gccagcttac cgggcgtttt accacgtcat agttggtttt tgccagctct
    59521 aacatacgct tttcaagagc gtctaaatct tcttgagtca aagagcggtc taaatcgata
    59581 tcgtagtaga aaccgttttc gatagtcggg ccgatagcca ttttaacgtc tgggaataat
    59641 tgcttgatgg agtgacctaa caagtgagcg gttgagtggc gaatgatctc taaaccatct
    59701 tcgtctttgg cagtaatgat ttcaagtgtg ctgtcttggc tgatgatatc agacgcatca
    59761 cggcgttcac cgtttacacg accggcaatg gtggctttcg ctaaacctgc cccgatagat
    59821 tgggcaactt ccattacaga aactgggttg tcaaatgaac gttgtgaacc gtccggtaaa
    59881 gtaataattg gcataataaa tccttacagt ggtaatccat acgagagatt acttgtgttg
    59941 tttgaatgaa aaaaccgcct acaagcggtc ttttttgatt aaaattttgc aatttgctta
    60001 ttatttccct tacaagtggg gaataaaagc actgaaattc tagcataaaa caaagaaaaa
    60061 ttaaactttg ctgttttcag cggccccttc atcaagctca atattttcat tttctgtttg
    60121 tggatcttgt tcaagtattt caagctgagc ctggtactct tgtaccgctt cttcatcaaa
    60181 tggaataagt gcgtcctcaa caacttcaat ttcttcaagt gggcttgcgg ttgcaattgg
    60241 ggtagcatat tgcatctctg ctcgttccgc tttcggaatt gatttggtgc ggaaagcaca
    60301 aataaataaa ctaagtgaga acagtccaaa aagtgcataa aagccatatt cagcggaaag
    60361 gctcatcgca aaaccgataa gcggtggagc aactaacgcc cccacaccat agcaaaacag
    60421 taaacccctg cttacttcaa ccgtgttcat attattcggc aattcatcgt tggcacgggc
    60481 aatgctcaaa gcatataagc taaacagact acagccaaag aaaaaggcga taatatactg
    60541 tccccaggcg gtcaaatttt cttggaaaat tgcaaataat ccggcaagtg cggttaaagt
    60601 ggcaatcact gaacaagcta aaatggcgtt acgtctgcca atacgattcg ataatatcgc
    60661 catcggcatt tggctgataa aaccaccgat catcgagatc atcaaataaa gcgagatttc
    60721 acttaatgaa aagctaaact tcagcagaaa gactgacgcc attgtaaaaa agccgtttac
    60781 caacattccg ctaataaagc tggttgctaa ggctaacggt gaaatcgcaa atagtttagg
    60841 aatactaatg cgctctcgag ccggaatatc aggctgaacc attttagtta aagcaattgg
    60901 gatcatcgac atcacaacca aaatggtaga taagataaaa atgtttcggc tactaagttc
    60961 taaacttaat aaacttacgc ctaaaataaa ggcgagatag aagataatgt tataagtggc
    61021 aagcactttg cctctggtat cgacattggt tttttcgctt aaccaacttt cgactaaaag
    61081 cagcaagcta taatgacaga atcccaaaat aattcgtaac acgccccaga tccacaaagt
    61141 atcaatcata atatgaccga gagcggcgat agcaaaaaca gcacagaaaa cgctaaatga
    61201 acggatatgc ccaacacttg atacaatgcg gtgagcaaaa atagtgctta atgccgcccc
    61261 aataaagaaa gcagcgttaa gtaaaccgat gatgatgtcc gttatcccca tttctgccag
    61321 tttgatccct gctgaattta gaaataaacc gtagccgaaa aacagaaaaa agatacctga
    61381 ataaagggag taaaatttag tgctattcat caatgctcct tcaaacattc tgccagtatt
    61441 aaatcattgt accgatttaa taccttttag gcaaaaaagt tattattagc gttgagaaaa
    61501 taacttttgg ctattttgat taaaaaatca aatgaaggtg attttgaaat gtagtaaaaa
    61561 atagagcaat cagaattgaa aaagggtttg ccgtaacaaa gcctttagaa agattagagt
    61621 ttcgagaaga atttgtagct tgggctaacc aaaacacttg ctaggagtgc tttaagcaca
    61681 ccacctaaaa taaatggata taagcccgct tgtaatactg tgccttctgg tacaaagaaa
    61741 gttgagccaa gccgaaagca aaggtgatca cacttgcaat taaggcgaca aataaacttt
    61801 tccaaaaaga gtttaatacg cctttatcgc tataatagcc taagaaatag gcggttggaa
    61861 tatagcctaa taagtatccg cctgttggac caaagaaatg atggaaacct gaagcaccgc
    61921 cggcaaagac aggtaaacca atcgcccctt caaaaagata aagtaatact gcagcagttg
    61981 cggctttacg acccattgca aaaccaacca cagttaaagc aagtgtttgc cctgtaatgg
    62041 gtacagggta catcggcaca gaaatttgtg caaaaatagc aagcagtgtt gctccaataa
    62101 aagttttcaa ccaaaatgta gttatactat tggcttctag ccaacgttta gtttgaatta
    62161 attgcgtagt gaacataatc ctctccttta atatggttaa aatgttctca accgattata
    62221 taagaagatg tcttttaggg taagagtgaa tttaaaatgg taaaaccagt ttatatttat
    62281 tgtgatgaag gtagcagtga aattggtgtt caatcactgt tattggcagt aaaacagcat
    62341 ttacagcgtc cggcttatct catcaatgcc aaacaaatta ttcaaagcaa gttttccgat
    62401 ggctgtttat taatcatgcc cggtggagcc gatttacctt actgcaaaaa attaaacgga
    62461 gaaggcaatc gtagaatccg ccaatttatt gaacaaggtg gagcttatct cggtatctgt
    62521 gccggagctt attatggctg tgctgaattg aattttgtag ggcgtgacta tagcgtaaat
    62581 ggtaatcgag aactagcttt atttaatggc attgcgggca ttgcgaaagg cagtctgccg
    62641 cagtttacca acgggcaact ttacgataaa aacattggta gcaaagcgat agttgaagta
    62701 acacttgcag agggtggaaa aatgccgttt tattatcacg gtggttgctg ttttgaacca
    62761 aattcaacaa cagtgattta caacccgttg gcttattatc ctgatggctc agtagcaatg
    62821 atagagggag agctagggcg aggttactat ctgttgtcgg gtgtacattt tgaattacaa
    62881 gcggtcgttt attggcaaaa aattgtaaat tgctgtgaaa atcttgacat aaaaacacaa
    62941 gaagaagcaa tttgctctaa ttttaatgta aattatggct cgcaaatttg gcaacatcta
    63001 aagcaacgac taaatcaagc gtaaaaaaat aacctttcgc tattctgcca aatggtttct
    63061 gcaatcagat ttggtgattc tgttcttaat tcacatagac tattgaaagt cactaaaagc
    63121 cgttccggtc ggttcggctg gccttgaaag ccaaacaccg gcatatcggg cgagtcggtt
    63181 tccagtagta aactttcaag aggtagtttg cggatagcct cacgggtttt gttagcacgt
    63241 tcataggtaa tagttccccc cacaccaatt ttataaccga gatcgacaaa acgttttgcc
    63301 tgttcatagc tgccggcaaa gccgtgaacc acaccggttt tggttagttg ggcttttttg
    63361 aggaacacaa aaagttgatc gtggctttta cgtgaatgaa gattaacggg taaattaaat
    63421 tttttcgcaa gggaaagttg tgcctctaaa aattcacatt gctttttcca taattcaggg
    63481 gtagaaactt cacttaccgc tttttccaaa ccgatttcgg caatagcggt acactgtgga
    63541 tcttgttggc ttagcaaatt ttccaataag attaaatctt gttgttggtg ctgttgaata
    63601 tacagtggat gcaaccctaa tccatacacc aaattttccg gtttttgttt ggcacaagcg
    63661 gtcacttttg caaaattttt tgcaaatacg gcaacgataa gaatgcattc aacctgatgc
    63721 tgtttggcat tctcaaccaa ttccggaatg cttaaattca ggctttctgc gagatagtcg
    63781 aggtgagtat gagtgtcaaa aaatcgcata ataaaaccaa taaaaacggg gactagattg
    63841 tccccatttt tagcatttta cgcaagcaat tagtttgtta cataagcatc ttcatcacga
    63901 gcctcagatg gcttgaggaa agcaaagaag caaataacac aaactaccgt aatcgcaaaa
    63961 ccgatataaa ctgataattc ataatctaaa ccgaagccga ttttgttgtt cgcaaggtaa
    64021 gttgcacaca cggtagtgat aaaccatgca ggaatcgagc atacccagtg gaatttttta
    64081 tagcggtata agtaacctgc ggcagtccat aacataacca ttgcagtcat ttggtttgcc
    64141 caagtgaagt aacgccataa tacgctgaaa tcaacttttg agacaatgaa accaagtacg
    64201 aataatggta cggcaatata caaacgtttt actaaggtac gttgttctaa accaaagatt
    64261 tccgctagtt gtaaacgagc ggctcggaat gcagtgtcac ctgaggtgat tggtaatact
    64321 acaacgccta atacggcaaa aataccaccg ataaacccta agaagtgtaa cgagctgtca
    64381 tataccactt tagacggtga accggcagag atcgcatctt gtaaggcttg tgggttttca
    64441 tagaatgaaa gacctactgc acaccatact aatgcaatta ccccttcagt aatcattgca
    64501 ccgtagaaaa taaaacgacc ttctttctca ttttgcgcac agcgagccat taatggggtt
    64561 tgggttgcgt ggaaaccgga taatgctccg caagaaatag ttaagaatag taatggccaa
    64621 attggaacat cacctttcac ttggaagttt tgggtgaatt tttcccacgt taagccttga
    64681 ccgtctgcat tcactgtacg gaagaagtcg ataggatcag tcgcacttaa gtgagcgctg
    64741 actagaccat ataccatacc tacagacata aagagtaata atgctccgaa gaaaggataa
    64801 attcgaccga tgattttatc aacaggtagt aatgtagcaa gaatatagta agcaaaaatg
    64861 attaacgtcc acacagaaat cacggttgct ttatccatac cccaaacggt tacgccacct
    64921 gatgctacag attctttaat tgcttcaaca tcacctaatt ccattgcact ttgggttgca
    64981 cctgcaatat ccatggtgat ggtagagagt aactgagcag ggcttgcaac gaaaactacg
    65041 cctactaata aaagcaagat gagcgccatt acgttgatga ataacttgac tggacgaccc
    65101 aagaatttcc ctgccagatt tggcatcgtt gcgccaccgt tgcggatact taacatacca
    65161 cagaagtagt cgtgtactgc accggcaaaa atacaaccaa tcacaatcca aagcattgca
    65221 actggtccgt ataaggcacc aagtattggt ccaaaaatag ggccggtacc ggcaatgttt
    65281 aataattgaa tcagccaaat cttggtttta gacattggca tatagtcaac accgtcgttc
    65341 attgcataag ccggcgttgc tcttttcggg ttaatcacga agattttttc aatgattttt
    65401 ccgtaagtaa agtagccaag aacaagcacg gctacacaga aaaagaacca caacatagta
    65461 gaaatcctat aaattgagat aaatgatcat ttttagcaga actgataaag gcgtgccatt
    65521 gtaagtgagg agtgaggata aagtaaaatg aattgttaga tttgtgtgaa taatttactg
    65581 aaaatgtgag gttcatcaaa aaatcccttt gttctgcgtc aatcaaaggg actttttaaa
    65641 gttaaatatc gaatttttga attttcgctt caatgccttg ggcatctaat ccaagatcgg
    65701 cataagcttc agcttgagta gattggggaa cgaaaaagtc aggaatacca agcattacaa
    65761 gcggtttgat tttgccgata ttttgcaaat attcattcac aaaactacct gcgccacctt
    65821 gaatagcatt ttcttcaagc gtaactaaca actcgtgtga atccgccact ttttgaagta
    65881 atgcttcatc taatggttta atgaaacgca tatctaccaa cgtgtagtta tgtttttcag
    65941 ccacaatctt tgcttcatca agcaaagcac caaaatttaa aatcgcaact tttttacctt
    66001 cacgaataat gtttccttta cctacttcaa gtctttgcat tggctcaagt tttacccctt
    66061 tagcattgcc tcgagggtaa cgaatagcac ttggcgtatt catcgtgtaa gcggtataaa
    66121 gcatttttcg catttcattt tcatcgctcg gtgccataat ggtcatattg ggaatacaac
    66181 gcataaaact taaatcaaat gcaccttggt gagtttgccc gtcagcgccg acaattccgg
    66241 ctcggtcaat cgcaaaaatc accggcaggt tttgaatagc aacatcgtga attaattggt
    66301 cataagctcg ctgcaagaag ctggaataaa ttgcgactac cggtttgtaa cctgcgattg
    66361 ctaagcctgc cccgaaagtc actgcgtgtt gttcggcaat cgccacatca aaatattgtt
    66421 cagggaaacg tttggaaaat tctaccattc ccgagccttc acgcattgcc ggcgtaatgc
    66481 cgataatttt cggatcacgc tctgccattt cacatagcca atcgccaaaa atattggaat
    66541 aagtaggtgc tgcttttgat tttggtaact cggctgcggt agggtcaaat ttcggaacgc
    66601 catggtagcc aatcggatca ttttccgcag gtgtgtagcc tttgcctttt ttcgtgcgaa
    66661 tatgcagaaa ttgagggcct ttcagctcac gcatattgct caaggttttg actaactcgt
    66721 caatattatg tccatcaatc gggccgatat aattaaagcc taactcttca aacaaagtgc
    66781 tttcagggga gaaaatcacc cctttcatat gctcttcgct ttttttcata aagtttttga
    66841 tggtaggcac tttatctaaa attttcttac tgccgtcacg cactgaagta tagagcgaac
    66901 cagaaaaaat gcgggcaagg tggttgttta atgccccgac attttcagag attgacattt
    66961 cattatcatt taagataacc aacatatcgg tatgcaatgc acctgcgtgg tttaatgctt
    67021 caaatgccat tccggcagtg attgcaccat caccgattac acagacggtt ttacggccgg
    67081 cattttcttt ttctgctgcc accgcaatgc caagtccggc actgatcgaa gtagaagaat
    67141 gcccaacgct taacacatcg taagggcttt cttcacgcca agggaagggg tgaataccat
    67201 ctttttggcg gatagtgtgc atttgatctc ggcgcccagt aaggatttta tgtgggtaag
    67261 cttgatgacc aacgtcccaa attaattggt cgaaaggcgt ttgataaaca taatgcaaag
    67321 ctaccgtaag ctctactacc cctaagccag aggctaaatg tccgctagtt tgactgactg
    67381 attccaataa ataggctcgc agttcatcaa ctacggtttg aagctggtct ttagaaagta
    67441 aacgtaaatc ttccggtgag ttgatttcgc ttaacagagg atacttcttt tccataatgt
    67501 tttattaccc acaaccatca aataatagat gatattaaat taagctttga gttcttcggt
    67561 caaagtaggc tgaatgctat ttaaaatttc tttaaccaca cggccgttac caacaaccag
    67621 attacctgtt ttcaagtaat ctgttccgcc aacaaagtta gtcacaattg caccggcttc
    67681 acgggcaata agttcaccgg cggcgaaatc ccacggtttt aagccgattt caaagaaacc
    67741 atcaacacga cccgctgcaa cataggcaag atctaaagcg gcagaaccgg tacggcggaa
    67801 atcagctact ccgttattca ttaagccttc aagcatattt aaatgtgcat cacgatgtct
    67861 gatgactttg aaaggaaaac cggttgcaat tacagtgcct tttaaatcac ggcgttctgc
    67921 atcaatgcgt aaacggaact cgtttaattt agccccttcg ccacgcaccg cagtgaaaag
    67981 ctcgttacgg ataggatcgt aaactacccc tactgttgta cgtccgtttt cacgcacggc
    68041 aatcgacaca gcaaaatgag gtaagcgttt tacgaaattg gttgtaccat ctaatggatc
    68101 gatcacccat tgtacatttt cattgctacc gtttaacacg ccggactctt cggtcacgat
    68161 cgagtgttca gggtaagatt tacggatcac atcaataatt gccgcttccg ctgctttatc
    68221 gatttcagtc acataatcat tcgtattttt ttgtaaaact tgtgtatctt gaggcgattg
    68281 ttcatagcct ttggcaataa tattgcctgc ttttcgtgcc gcacgaatag caatgtttaa
    68341 cattgggttc atatttccac cagattttaa agaacataat aaagcacgaa ttataaagaa
    68401 tgtagcgaaa taaagaaaca acaagcggtc taatctgtga aaaattttgc aaaaaaatgg
    68461 ctttttctaa ccgcttggag tttggattcg ttataatacc tcccattcaa gccacccatt
    68521 taggtactaa aaatgcaatt taaagaaaat aaccaacctc gttttaaaca cagagaagaa
    68581 cgccgttcag atgagtgttc cccccgtttt tcagataaag gcaaaatgcg aggaaatgaa
    68641 ggtaaaagtg acagaccgca taagctacat tttaagcgtg acgaggctta ttcacgctct
    68701 gagcagattt atccagcaga aggcaaaaaa gccagtggtg aaggtagtgt gcaaatttgg
    68761 gtaaaaggtg ggaatagctc agttaaagag aaaaaaaacg gcccgctttc accgagagcg
    68821 cctgagaaaa ttaagaaaaa tcgcttggaa gagatgaaag tctatggcga aaatgcctgt
    68881 atcagtttgt tccaacaacg tcctgaatct attgtacgtt tatgggcaac agtggagggg
    68941 gcaaaaaaat taggcgattt aatgagctat ttggctcagc acaaaaaagc ctatcacgtt
    69001 attgaccgtg aagagatgga gcgagtcacc ggtactgaac atcacggcga tatgtgctta
    69061 ttggtgaaaa aaacggcttc atttgtgcta gagggctatt tacaagtgcc gaaaaaacaa
    69121 gattgcttgg tactgttaga tggtgtaaac aatgctcaga atgtgggggg aatcatcaga
    69181 acgtgtgctt tttatggtgt gaaaggcatt attactgaaa atgtggatag cttaaactcc
    69241 agtgctgcag ctcgtgtcgc agagggtggt ttggaatttg tgcgagcgtt agaaactaaa
    69301 aacaagcata ttgctttggt acaactccgc caagccggtt atcaaattgt ccacctcaca
    69361 cgccataagc aagccccttc tttaaccaaa attaagctgg cggaaaaggt ggtttttgta
    69421 ttaagtgaga ttcctgcaag tgatgtggct tacgcagaag acacaacaat acagctttcc
    69481 tttaataatc cgttaaatag tggtttaaat gtggcggtaa acgcaggtat tctacttaat
    69541 caatggtatc aaacccacat tctttaagga attgaaatga atcagcaaaa accgtttgaa
    69601 gtgattgcga ttgttggcac acctcgccac gacagtgcat ttgaaacaca tcttgccgtt
    69661 tataattggt taaaagatcg tgattatcaa attttagtgg aaacagaggt tgccactcag
    69721 ttacagctta aggaaagcta cagtttagat cagattgggc agcgcgcaga gttggtgatt
    69781 gtgataggtg gcgatggtaa tatgcttggc atcgcccgag ctttggctaa ataccgagtg
    69841 ccactgatag gcattaaccg tggcaatctg ggttttttaa ccgatatcgc acctcaaacg
    69901 gcttttgaac agctttatag ttgcttagtc aaaaatgagt atgtgattga agagcgattc
    69961 ctgttagagg ctttcattga gcgcaacggt aaaattattg ccgcaaataa tgccttgaat
    70021 gaaattgtgg ttcacccaac ccaagtggct cgaattattg aatttgaagt ttatattgac
    70081 ggaaaattcg ctttttcaca acgtgcagat ggcttgattg tggcaacgcc tacaggctca
    70141 accgcctatt cactttctgc tggtggcccg attttaaccc cgaatatgaa tgcaatggca
    70201 ttagtgccga tgcaccctca cactctttct tctcgtcctt tggtggttga tggcgatagt
    70261 caaatttcgc ttcgttttgc caaatataat acccctgatt tagaagtgat ttgtgacggg
    70321 caggtgaata tgcactttac gcctgaagat agagtcttgg tgcgtaaaag cccggataaa
    70381 ttacagctgc tccatttaaa agattatagc tactttactg tattaggttc taaattaggc
    70441 tgggcaagta agttgtttta aacacaagcg gtgttttttc tgattaaatt tgcaaatgca
    70501 atatgaattg atttcatata catttcaaaa atatatcatt tttattcaat aaaaatttgt
    70561 ggttcaataa gctatctgaa aaataaaggg gacaataaaa tgacaacaaa ttttaaatgc
    70621 caagtgcata ctattgaatt taatgaagat taccaaccgg agcaaagcac ccgtttaacc
    70681 accaactttg ccaaccttgc ccgtggcgaa aaccgcaaac agaacctgca aaatgcgatt
    70741 gcgatgatca ataaccgttt taacgattta gcgaactggg ataacccgaa tgcggatcgt
    70801 tattcggtga aattggatat tgtctcactg gatattgatg tggaaggcaa aggcgagttt
    70861 ttccctgcga ttgagtggct gaaaacctat gttatcgact acaaaaccgg caaaaccatt
    70921 gatggcatta tcggtaataa cttctcttct tttgtgcgtg attacgattt cagcgtattg
    70981 ttgttagatc acaataaaga caaaccgcaa tttagcgtgc cggaaggcta tggcgagtta
    71041 cacggcaagc tgtttaagca tttattggca tcgcctgaat ataccgcaaa atataaaaaa
    71101 ccaccggtga tctgtttaag cgtggcaacc acccgtacct acacaagaac cactaacgtt
    71161 cacccaattt taggtgtgga atatctgcaa gatagttatt cattaactga tgcctatttt
    71221 gcgaaaatgg gcttaaaagt acgttacttt atgccgccaa aatccgttgc accctatgcg
    71281 ttctactttg atgagaatgc agatttactc aatgattaca ctaacttaga gctaatcagc
    71341 acgattgcca caatggaagc gttccaaaaa atttaccgtc cggaaattta caatgccaat
    71401 tcggtcgcag ggcaggtcta taaaggcagc ctgacttacc aagattattc cgtcaccaaa
    71461 gtggaatatg accgtgaaga acgcacccag ttggcgatca aacagggtaa atacaccgaa
    71521 gagcatttta ttaaacgtta ccaagcgatt ttagaggctt gggcgacgga ggaataaacg
    71581 tagggtgggc gtaagcccac cataaaatga gatataacca atggtgggct tacgcccacc
    71641 ctacttttta taggaaaatt caaaatgagc aacattcttc tcccaacctc taccgccggc
    71701 agcctaccaa aaccttcgtg gcttgccgag ccggaaaaac tgtggtcacc ttggaaattt
    71761 gaaggcgagg aactacttca agccaaacgt gatgccctcc ttgtttccct tgccgaacaa
    71821 attgcggcag ggattgatat tgttagtgat ggcgaacaga cccgccagca ctttgtgacc
    71881 acctttatcg agcatttgga aggggtggat tttagcaagc gtgaaatcgt gcgcattcgt
    71941 aaccgttacg atgccagcgt gccgagcgtg gtgggggcag tttcccgccc gaaatcggtg
    72001 tttgtggacg atgccaaatt cttgcgtgcg cataccgaca aaccaatcaa atgggcatta
    72061 cccggcccga tgacaatgat cgacactctc tatgacggtc attacaaaag ccgtgaaaaa
    72121 ttggcgtggg aatttgccaa aattctcaac gaagaagcca aagagttgga agcggcaggg
    72181 gtggatatta ttcaatttga cgaaccggca tttaacgtct tcttcgatga gctgaacgac
    72241 tggggcgtgg cggcgttaga gcgtgcggcg gaaggcttaa aatgcgaaac ggcggtgcat
    72301 atctgctacg gctacggcat taaagccaat accgactgga aacagaccct cggcaacgaa
    72361 tggcgtcaat acgaagagag tttcccgaaa ctgcaacaat caaaaatcga cattatctcg
    72421 ttagagtgcc aaaactcccg tgtgccaatg gacttaatcg aattagtgcg tggcaaaaaa
    72481 gtaatgctgg gagcgattga tgtggctacc aacattatcg aaacaccgga acaagtggca
    72541 gatacgttac gcaaagcatt acaatttgtc gatgcggata aactttatcc aagcaccaac
    72601 tgcggaatgg caccactttc acgccaagtc gcacaaggta aattacaggc actcagccaa
    72661 ggggcagcga tattgcgtgc ggaattcgca aaataatttt gcgatctgca tcgaaaatat
    72721 caactttgct ttttgctcgt ttccagattt tcttccaaaa taacaggaaa tgacggaggc
    72781 actatgctaa atctatttaa aaagccaatt aagaaaagct cctttgaggc ttgggcaaaa
    72841 ctaatggacg atgtggcaaa agttgcaatt ttagcaattc ctgttattct ctatggagaa
    72901 tattcactgc taatgaaatt tacacactca attaccttat ttgcagtagt ttattttttg
    72961 ttagcaacgg caagattgtt tcgagaaaca gcagaggaga tgtaaatgga cggattgaca
    73021 atcggattat ttcttttgct tgcattttca tttgctgcat accaatgggc aaagcattta
    73081 aaagatcata atcattaatt caatgaataa tactaagcgg tcatatttgg taaatttttt
    73141 acaaaatgtg accgcttgtt tttctttcac tcgaagttaa tttttatata aataatacta
    73201 tctatgaaac taaaaaatgc gtaaaataac gtgaaatcaa cttacgcaaa cgtttactta
    73261 agcttattag gagtccaaaa atgttaaaac gtgatatgaa tatcgctgat tacgatgcag
    73321 tattatggca agccattcaa gatgaagatc gccgtcaaga agaacatatt gaattaattg
    73381 catctgaaaa ctatgcaagc ccacgtgtaa tgcaggctca aggctcacaa ttaaccaata
    73441 aatatgcaga aggttatccg ggtaaacgtt actatggggg ttgtgagtat gtggatattg
    73501 tagaacaact tgcgattgac cgtgcgaaag cattgttcgg ggcagattat gccaacgttc
    73561 aaccgcattc aggctcacaa gcgaatgcag ccgtatatat ggcgttgctt aatccgggtg
    73621 acactatttt aggtatgagt ttagcgcacg gcgggcattt aacccacggt gcttcagtta
    73681 gcttctctgg taaaatttat aaagcagaac aatacggcat tactgatgaa ggcttaattg
    73741 attatgaagc attacgcaaa caagcccaag aagtgaaacc gaaaatgatt gttgctggtt
    73801 tctctgctta ttctcaagtt atggattggg caaaaatgcg tgagattgcc gatgaagttg
    73861 gggcatattt atttgtggat atggctcacg ttgcaggctt aattgcggcg ggagtttatc
    73921 caaatccgct tcctcacgct cacgtcgtga cgacaacaac ccataaaacc ttagccggcc
    73981 ctcgaggtgg tttgatttta tctgcatctg gcgatgaaga aatgtataag aaactgcaat
    74041 ctgccgtatt ccctgctggt caaggtggac cattgatgca cgttattgcg gcaaaagcgg
    74101 tttgttttaa agaagcttta gagccggaat ttaaggcata tcagcaacaa gttgtaaaaa
    74161 atgcaaaagc aatggttgag gtatttaagc aacgtggttt tgatgtggtt tcaaacggta
    74221 cggaaaacca tttattcctt gttagctttg taaaacaagg tttaaccggc aaagcggcag
    74281 atgctgcatt aggagctgcg aacattaccg tgaataaaaa ctcggtgccg aacgaccctc
    74341 aaaaaccatt cgtgacttca ggtattcgtg tgggtacgcc ttctgtgacc cgccgtggtt
    74401 ttaaagaagc agaggtgaca gcattagccg gctggatgtg cgatgtgcta gatagcattg
    74461 gtaaggataa tcacgaacag gtgattgcag agactaaagc gaaagtttta gacatttgta
    74521 aacgtttacc agtgtacgca taatctcaac tttacacttt ttcaagcggt aatatttttt
    74581 agaaatatta ccgctttttg ttttatcaat cattttcttt atctgttttc tgaaaatgga
    74641 ttgatttttc tttttttaca taacataatg ataataattc tcattaattt attttttgtt
    74701 atgttacctc gtatttcctc ttttttagct agccttttta tggctttttt tcttttaggc
    74761 tgctcggttt caacgcaatc ccttaatgaa caaaacgtgg actctatcgg ccaaattatt
    74821 gatctgaata ccaatcaaac gatttcattt actgaactac tttcccgtct atcttctcaa
    74881 gattacgttt tagttggtga ggagcatact cagttaattc accaccaaat tgaattgtat
    74941 ttatttaatc aacttacgaa gaaagccaag ttacatttaa ttgcgttgga gatgttaaat
    75001 gtggatcagc agcctgcgat tgatcgcatt cagtttaacc gaccggttaa tttatcagcc
    75061 acggatttaa gagaggcaat tcaatggcaa aaatgggatt ggaagatgta tcaagattta
    75121 gtggtagaga gcttaggttc aaatagtcgt gttatagcca ctaacttaac agataaagag
    75181 gttgaaattt tattaaatgg ggcggagcca ttaaaaggtt ctgtatccac ttcctctgaa
    75241 gttaagcaaa agatcgcaca actgttgctg tcaatgaatc acggaatgcc ttacccgatg
    75301 gaaaatatgg tggcagtaca acaattccgt gatcgcagaa tggcagaaaa attagtaaaa
    75361 aatgcgttat cgactagctt gctgattgca ggtaaccacc acgttcgcaa agatcttggc
    75421 gttccgttgc atattgcgga atatgaccga acaaaaaaag tagcggtgct aatgcttaaa
    75481 acagagagag aagaaattac ttcttcacag gcagattatc tttgggtcac tcaataagga
    75541 gaattatgaa atttaatata atcacacgca ttttgtttcc ggttttgtgt atgggaggat
    75601 cgcaaactgc attggcagag gaaaaggcga aaaatattgc ggagcttgat actgtgacgg
    75661 tttatggcac tgtggaaaac cctaattttg agaatttcag taaagccggc tcggtgagtt
    75721 ctcgagcaat gaatgtgaat gcaatgcaaa gtttagatag tattgtccgt tctgtcgcag
    75781 gtggctatac ccaaattgac ccggggcaag ggggcgtgag tgtgaatatt cgtaatatga
    75841 gtggtcttgg tcgggtaaat accttagttg atggcgtgcc acaaacacag cttggtacat
    75901 cagcaaacgg aggtggtaag ttccacactg atggtaacgg gccaatgagc caattcagca
    75961 ctttaattga tcaaaatttt ttaacccgag ttgatattgc gaaaggctac aacaatggtg
    76021 cagccggatt gaatgcttta gccggtagtg ctaattttaa aacgattgat gtcgaagata
    76081 ttctactaga acaacataaa gtcggcgtat taagccgttt taattatggc aataatgatc
    76141 tgggaaaaaa cggaatgctt gcactagccg gaaaagttga tgtgctggaa agtggttctc
    76201 ttggcagttt attggctttt agcaaaagta gtatcagtca aaattatcgc cgaggtaatg
    76261 gcacaatggc aacggattct gcttatatgc aagggttaaa aaatcaccct gaatcttata
    76321 tgggaaaaat tgcctttaag ccaaatcaag attcagagtt taaattgtcc ggacgtcaat
    76381 ataaaaatag cttagtgggg cgtaatattg atagtgtgac ctattcctta gaatccagtt
    76441 accagcctca atctgattgg attgatctga aattcctaac agcagtaaca gaaactaagc
    76501 aagatttcca cgatgatgca aaactatgga cattagaaga tgcctcaacc aaaaacaaaa
    76561 tgtggatgtt tgatttgaat aacaccagcc gttttgagtc agggaaataa atattcccgt
    76621 gatttaccgg aaagtaagaa taatgatgga gcgattagca atgccgcttt tgggccggca
    76681 ggtaagcaaa atattgattc cctttaccta gacaccgaat ggaaatacgg cattgtcact
    76741 ttaaattcaa gtcttcacta ttcacgctat cagttaaaag gatataagcc agaatgcgat
    76801 agcgatgata tggattattg tttcccttct tttgggtttg atgttaataa aaaacattat
    76861 aagttgaatc cttctgccac tttagcattt caggttgctg attggtttca accttttgtc
    76921 agttacaaac aaacctcccg ttcgcctaat gtgcaagaaa tgtttaatac cagtcgaaat
    76981 ggcttgagtg tgaacccgtt tttgaaacca gaaagtgcga aaacctatga gattgggttt
    77041 aatattcatc aagataatgt attactcaat aatgatatct tggggctaaa agcggtatat
    77101 tttaactcac gcattaaaga ctatttgtat aacctctcgt tgtatggttg tgggccaaca
    77161 gtatgtcgaa attcaagtga tgctaccaat gctatacagt tccaaattta tgtgaatcac
    77221 cctgaaaagg ttaaaaatcg aggttgggaa gttgagctgg cttacgattc cgatgctttt
    77281 tttgtcaatg gtagttatac ccgaactgaa agcacagcac ccacttcaaa aggtgcgact
    77341 attctttatg gatttggcac tgaagcttat acccagctac caaaagatta tgcgactttg
    77401 gagctagggg ctaaattgct ggataaaaaa ttaacactgt ctagcatttt taaatataca
    77461 ggcaaagcga aacgtgtcgg tattgattta gacgaagatg gcaacattat tcacgagcag
    77521 ttaccgaata ttccggtgat tatcgattta tatgcaaatt acaaagtgaa taaacattta
    77581 accttaaaag ccggggtaca aaatgtgatg aaccgaaatt atcttgatgc cttaaatgcg
    77641 tataactctt ccaccgcaga agagtatgac tatgatacag acacctatcg tttcacaaat
    77701 agtgccagag gtcgaactta cacactaggg gcagaggtgc gtttctagct tgaaagtggc
    77761 ttattttaac caatacgccc tttttctttc ctgtactgat gttgagattc aaggcataat
    77821 atccagaacg atgaatattc agccggtgtg taatatgaaa aaatttgtgg ttttaatttc
    77881 agggaatggt tctaacctac aagcaatgat tgatgcgcaa aaatctgcgg atacaagcgg
    77941 tcaaatttgc ggtgtaattt gcaataaggc agatgcctat gggctgattc gagctaaaca
    78001 ggcgggaatc ccgacatttg tattttctcg taaagattac caaagcaatg tggaaatgga
    78061 tcttgcgatt gccgaacaaa ttgagcagtt aggagctgag ttaattgtat tagccggcta
    78121 tatgaaaatt ttaacgcctg aatttacgca acattttgct ggcaaaatac tcaatattca
    78181 cccttcctta ttacctaaat acccagggct aaacacttat caaagagcga tagaggctgg
    78241 ggaaagtgaa cacggcacga ctattcattt cgtgaatgaa gaagtggatg ctggtgcggt
    78301 ggtattacaa gctaaagtgc cgatttatcc tgaagatgag attgaagatg taatggctcg
    78361 agtggttgag caagaacatc gatattatcc gttggtgatt gagtggttct gttcgggtag
    78421 attagtttct cagcacggca aagcgtattt agacggtaaa ctactgcctg cttgtggata
    78481 tgcagaagat taaacgatag ggtgcatcgc accctaactt tcagctaatg cagggcaaag
    78541 ataatattgc ccgctgtgag ggcaatagcg acagcccatt ttcatttcat acagctcatc
    78601 taataaacaa ctttctgcaa tggctttagc ggacatttca gcaaattttt ctccattttt
    78661 tagcattaag atatgatcgg cttgatttat tgccaatgca ggatcgtgca aaatcgcaat
    78721 gatagttaag ccttgttgtt tcagcttttg tagtaaggaa aaaagctgat attggtgatg
    78781 aatatccaaa tggttggtgg gttcatcaag caaaagccag cttccgctta aatcggcatt
    78841 cggttcagga acgagttgag ccagcactcg agcaatatgc gctcgatgtt gttctccacc
    78901 ggaaagttgt atgctacttc tttcttttaa tgcggttaaa gacattgttt ccatagccca
    78961 agcggttatt ttttcattaa attttgcaaa ttcactttgg cgatatggct ctcttccgag
    79021 ttgtaccaat tcttctactt ttaagggaaa ggcaatttgg ctctgttggc tgagcattgc
    79081 ccgtttttga gccagagcat atccgtttaa gtctgttaag cgttgatgtt gataaaatac
    79141 ggcactgtta gtttgtgcat tattaccgct taaggctttg agcaaggttg ttttgccggc
    79201 tccgtttgga ccgatgatgg cggtaaattt tccttgttca aaggcaattt cttgaatctc
    79261 cagcaacttt ttttgttgat gaacgatgct taaattttgg caagttaata aattgctcat
    79321 tagatatgtt tcccactttt ccataccatt actgcaagga atggcgcacc aaataaggcg
    79381 gtaaaaatac caatcgggat ttctgccggg gcggctaggg ttctggcaag agtatctgca
    79441 atcattaata agattgcacc gcaaaccaaa ctattgagta ataaatggcg gtggttactg
    79501 cctcccagta aacgcattaa atgtggaata accagcccga caaagccgat agccccaaca
    79561 caggcaacgc ttgtgccggt taataaggca acgtaaaaca caagacgacg tttgactttt
    79621 gcaatgtcaa aaccaatgta ttgtgcatct tcttcaccta atgttaaggc gtttaacgca
    79681 cggtgttccc gccaaataag tgggctacaa atgaagacaa ccgcagccat cattgccacc
    79741 atttgccaat tcatacctgc taaagatccc atactccaaa aggttaaact acgaagttgg
    79801 ttatcatcgg caacataaat cagtatgccg gttaaagcgc cactaaatgc agctagagca
    79861 ataccaatta atagcataat ggcgatatgt agttgcccgt tacgttgaga aagtaaatag
    79921 agcataaaag tcaccagcca gccccctaaa aaggcggcaa taggtagagc ataatagctg
    79981 attgattccg gcagtagtgg ctgaatcacc acaatatata atgccgcagc caatgcagca
    80041 cctgaagaga caccaataat ccccggatct actaacggat tgcgaaacag cccttgcatt
    80101 gttgctccgc taatagcgag ggctgccccg atgaataatg cccctagtaa tcgaggcagg
    80161 cggatttgcc ataaaatata ggctgcactt tcgcattcag cgttgttgca gtgccaagcg
    80221 gataggggta atgacatcgc ccctaaatgg gtacaaatca ctaagctgat aattaaagtg
    80281 aaaattaagg ttaaatgcag tttcaaagcg tatccgttat tttaaacaag gttgaatagt
    80341 ggtgagaatt tggtgtgcat attgagcagt gtaaggccca aagcccaagg catctaaaat
    80401 attgattgca aaaacacagc cttttttgcc cgcttgtgta tttgtgagtt gggcgtattg
    80461 tgggctggat tctgctaatt tactgatcgt tgcagattct tttgcctgat tacgggaggc
    80521 taataaaata acatcggcat tgcttgcagc taaagcctca ttggcgagtg gcttattgcc
    80581 ctcagcattg aaactattgt ttagatttaa caaggttaag aaatgatcac ccacactctt
    80641 tttccctagc ccaaagatac cttgcgatgc agtgtcaatc agcactaaga cttctttgtc
    80701 agtagaatgt tggctacgaa tgtcggctaa ttttttttca tctgccagaa tggtttctgt
    80761 taattgtttt gcctctgatt ctgcaccaat cgccttggca atttgctgaa tatcctcacg
    80821 aatccctgct aagctcggtt tctctcctaa gtgaagtaat ggaataccgc tggctttaat
    80881 ttgctcaata actgcgttag ggccggcatc aggggctaaa ataatcaaat ctggggttaa
    80941 agagagaata ccttcagcag aaagctggcg acgataaccg attttaggtt ttttctccat
    81001 tatcgtttga ggtttaactg aagtggtatc cacaccgact aacttatcag cttttcctag
    81061 tgctgcgaca atttcggaag catagcccgc agttgaaaca attttttctg ccgcctttgt
    81121 ggtatttaaa cacattgata gggcaatgaa agaaaaaagt aaggttctca ttttatactc
    81181 catttgattt ttatctaaac ttaaataata ataattatta tttcaatttt caattaaaat
    81241 gatatagtgt taacattaaa cggattaaac taactagagg atctaaaata tgcaaataaa
    81301 gcaaactgta ttagcaatag cgattactcc ctttatttct caactctcct tagctcaaga
    81361 aacaaagatt agtagcattg aactagatac tattgaagta aatgagcagc aggagaacct
    81421 acttaaacag caagcggtga tgagcaatga agatttagta cgttatagta ctgatatggg
    81481 cgttccgcaa acaggccgct ttggcccaca agggtttaat atccgtggtg ttgaaggtaa
    81541 tcaagtggca gtaacagttg atggcatatc gcaagcagca tcaaataact atgccacctt
    81601 tagtcgctat agttactaca ataaatctcg ccctgaaatc gatactgaat taatgagcga
    81661 tgtaaccatt gaaaaaggct caagcacaaa agtaggcaat ggggcattag gcggctctgt
    81721 gaactataaa actaaaaccg taagtgatat tttaatgaaa ggtcgccaat ttggtgggat
    81781 gttgaaatat acctataacg gtcaaaataa agagaatatt gagacggcag gtattgctgc
    81841 ggatggtcaa tatgcggatg ctttggttat gtatagccac cgtcaaggac acgaaacaca
    81901 aacaacgtct aaaggtccgg atattatggg aagtggtcgt ggtttaccaa atccttacaa
    81961 aaataatcaa tacagctact tggcaaaatt agggctaaaa tggaatgatg aaaatcgcct
    82021 tgaattaaaa acttctaagc aacgcagtaa aaacgccggt caagagcttt cttattctac
    82081 cgtcacatta cgtgattttt tggatgtgca ggaaattgaa acttatggcg ttacttattt
    82141 atacactcca aaaaacagta ttcttgaaaa tattaaagtt gcggtaacca aacaggatac
    82201 tgaaatttca ggtattaacg atcaaatcac tattgcccgt ggtacaaaaa cagataatac
    82261 caaacgggct tttaatacta aaaatgttga aggtagctta ggtgttacat tcaagcctgt
    82321 tgatctaggt aataccgaac ataaattgtc aacggaatta ggctatggtg tatctgattt
    82381 cagttttgat atgcaaacct atacagcagg tattacgcca accgatcaaa atatgcagtc
    82441 tccggcaaaa accaagtctt tccatatttt gatgggagac tctattttac tgacacaaaa
    82501 tctatctgct catttaaatg ctcgatatga gcagtcgaaa ttacattctg atgataatag
    82561 tttgtcagat aaaaccttta acaattgggc gggagatatt ggtttaaatt atcagctctc
    82621 tcctgtatgg caaattggct ataaactcag ccgtggtttt agaacaccta ccgcaagtga
    82681 aatgttcttt aaccgtgaag ttcaagggcg tggagcaagc caaactttct tagcaaatac
    82741 taacttaaaa ccggaaacaa gcttaggtaa tgaaatttca ctggtaggta atggttcatt
    82801 aggtagcttg agcgtagccg cttaccatac ccactataga aatctgattg atcttgccac
    82861 tattggtaac aatatttacc aatcgcaaaa tgttaaccga gctaaaatca aaggtgttga
    82921 tattaacgga actttagact taaacacagc gtggaatgca attccacaag gctttgagct
    82981 taatggtgga tttggttatg ctcgtggcaa acgtaatgat ggtaccgact tactcacggt
    83041 tcaaccgtta aaagcattgt tcggcatagg ttatcgagct gtgaacaatg actgggcgat
    83101 acacctaaac ggcacataca cacaaggcaa aaaagcaaaa tatgcacaac agtatgctat
    83161 tttagccaat actgggtatt taagttacga taaatcatta accacttacc catacttaag
    83221 taattcagcc accgtatttg acttagttgc acacaaaaag tttggagaaa gagcgattgt
    83281 taaagttggg gtttataatc tctttaataa aaaatattgg acttgggata gtttaagaac
    83341 tattggtata actagtcctg gagttgccaa cagtataact ggcgaaggct taaatcgtta
    83401 ccttgctcca gagcgctatg taatggcatc agtggagttt
//
LOCUS       DANLUQ010000002        44489 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.002, whole genome shotgun sequence.
ACCESSION   DANLUQ010000002 DANLUQ010000000
VERSION     DANLUQ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 44489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44489
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.002"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..2855
                     /locus_tag="PXB27_000087"
     CDS             <1..2855
                     /locus_tag="PXB27_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RTX family hemolysin"
                     /protein_id="HDL5344925.1"
                     /translation="RLTTLSNGLKNTLTATKSGLHKAGQSLTQAGSSLKTGAKKIILY
                     IPQNYQYDTEQGNGLQDLVKAAEELGIEVQREERNNIATAQTSLGTIQTAIGLTERGI
                     VLSAPQIDKLLQKTKAGQALGSAESIVQNANKAKTVLSGIQSILGSVLAGMDLDEALQ
                     NNSNQHALAKAGLELTNSLIENIANSVKTLDEFGEQISQFGSKLQNIKGLGTLGDKLK
                     NIGGLDKAGLGLDVISGLLSGATAALVLADKNASTAKKVGAGFELANQVVGNITKAVS
                     SYILAQRVAAGLSSTGPVAALIASTVSLAISPLAFAGIADKFNHAKSLESYAERFKKL
                     GYDGDNLLAEYQRGTGTIDASVTAINTALAAIAGGVSAAAAGSVIASPIALLVSGITG
                     VISTILQYSKQAMFEHVANKIHNKIVEWEKNNHGKNYFENGYDARYLANLQDNMKFLL
                     NLNKELQAERVIAITQQQWDNNIGDLAGISRLGEKVLSGKAYVDAFEEGKHIKADKLV
                     QLDSANGIIDVSNSGKAKTQHILFRTPLLTPGTEHRERVQTGKYEYITKLNINRVDSW
                     KITDGAASSTFDLTNVVQRIGIELDNAGNVTKTKETKIIAKLGEGDDNVFVGSGTTEI
                     DGGEGYDRVHYSRGNYGALTIDATKETEQGSYTVNRFVETGKALHEVTSTHTALVGNR
                     EEKIEYRHSNNQHHAGYYTKDTLKAVEEIIGTSHNDIFKGSKFNDAFNGGDGVDTIDG
                     NDGNDRLFGGKGDDILDGGNGDDFIDGGKGNDLLHGGKGDDIFVHRKGDGNDIITDSD
                     GNDKLSFSDSNLKDLTFEKVKHNLVITNSKKEKVTIQNWFREADFAKEVPNYKATKDE
                     KIEEIIGQNGERITSKQVDDLIAKGNGKITQDELSKVVDNYELLKHSKNVTNSLDKLI
                     SSVSAFTSSNDSRNVLVAPTSMLDQSLSSLQFARAA"
     gene            2930..5056
                     /locus_tag="PXB27_000088"
     CDS             2930..5056
                     /locus_tag="PXB27_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_063101322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HDL5344926.1"
                     /translation="MEANHQRNDLGLVALTMLAQYHNISLNPEEIKHKFDLDGKGLSL
                     TAWLLAAKSLALKAKHIKKEISRLHLVNLPALVWQDNGKHFLLVKVDTDNNRYLTYNL
                     EQDAPQILSQDEFEACYQGQLILVTSRASVVGQLAKFDFTWFIPAVIKYRKIFLETLI
                     VSIFLQIFALITPLFFQVVMDKVLVHRGFSTLNIITVALAIVIIFEIVLSGLRTYVFS
                     HSTSRIDVELGAKLFRHLLSLPISYFENRRVGDTVARVRELDQIRNFLTGQALTSVLD
                     LLFSFIFFAVMWYYSPKLTLVILGSLPCYILWSIFISPILRRRLDEKFARSADNQAFL
                     VESVTAINMIKAMAVAPQMTDTWDKQLASYVSSSFRVTVLATIGQQGVQLIQKTVMVI
                     NLWLGAHLVISGDLSIGQLIAFNMLSGQVIAPVIRLAQLWQDFQQVGISVTRLGDVLN
                     SPTEQYQGKLSLPEIKGDISFKNIRFRYKPDAPTILNNVNLEIRQGEVIGIVGRSGSG
                     KSTLTKLLQRFYIPENGQVLIDGHDLALADPNWLRRQIGVVLQDNVLLNRSIRENIAL
                     SDPGMPMERVIYAAKLAGAHDFISELREGYNTIVGEQGAGLSGGQRQRIAIARALVNN
                     PKILIFDEATSALDYESEHIIMQNMQKICQGRTVILIAHRLSTVKNADRIIVMEKGEI
                     VEQGKHHELLQNSNGLYSYLHQLQLN"
     gene            5068..6504
                     /locus_tag="PXB27_000089"
     CDS             5068..6504
                     /locus_tag="PXB27_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_140441352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HDL5344927.1"
                     /translation="MKIWLSGIYEFFLRYKNIWAEVWKIRKELDHPNRKKDESEFLPA
                     HLELIETPVSKKPRLIAYLIMLFLVVAIVLASVSKVEIVATAPGKLTFSGRSKEIKPI
                     ENAIVQEIFVKDGQFVEKGQLLVSLTALGSDADIKKTMASLSLAKLENYRYQTLLTAI
                     EKESLPVIDLSRTEFKDSSEEDRLRIKHLIEEQYTTWQKQKTQKTLAYKRKEAEKQTI
                     FAYVRKYEGATRIEQEKLKDFKALYKQKSLSKHELLAQENKLIEAQNELAVYRSKLNE
                     LENDLLNVKEELELITQFFKSDVLEKLKQHIENERQLRLELEKNNQRRQASMIRAPVS
                     GTVQQLKIHTIGGVVTTAETLMIIVPEDDVLEATALVPNKDIGFVAAGQEVIIKVETF
                     PYTRYGYLTGRIKHISPDAIEQPNVGLVFNATIAIDRKNLTSPDGRKIDLSSGMTITA
                     EIKTGERSVMSYLLSPLEESVTESLRER"
     gene            6745..7743
                     /locus_tag="PXB27_000090"
                     /pseudo
     CDS             6745..7743
                     /locus_tag="PXB27_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250818.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            7760..8503
                     /locus_tag="PXB27_000091"
     CDS             7760..8503
                     /locus_tag="PXB27_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5344928.1"
                     /translation="MKQKYIYGLAGLLLLLLGWQLGTALLSQRLPMASMLAPAKTWES
                     FTWLISSGEIFPHLWASLQRIAVGLLAALVIGVPLGLLLGFSKHLEMLCTPSFQLLRM
                     ISPLSWMPIIVMLLGVGDLPIYFLLAFATVWSLIISTTSGVKSIDPQWLQLGRVMSAT
                     RKELLCKIVVPAITGHVLTGLRVAVGIAWVVLVPCEMLGVNQGLGYFILDTRDRLAYS
                     ELMAVIIIIGLIGWALDSLIRLALAKGGR"
     gene            8658..9716
                     /locus_tag="PXB27_000092"
     CDS             8658..9716
                     /locus_tag="PXB27_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HDL5344929.1"
                     /translation="MSVLSSSFIQWLQQNAENLDQSNERADLLIPQIAALGLYKIGVP
                     ETLGGSGGTLVDAIKAVTELGNHSLAAAFVSWGHRTFIEHIIASKNPIPRQSYLADLL
                     NGNLTAGTGLSNAMKFLSGIEELNVNIREENGKRYLNGRLPWVTNLRSYRFVASFVAG
                     FQDERKPLVIAIPSTAKGLTRTKELEFIALQGTNTTSLVFENVELQDEWILADDAPEF
                     LAATRPAFLGLQFGLPFGLVERSLAEIEKSLPFRAILASEWQAQVDALNDIKTRLFNG
                     LNEEGYFVRNPKELFQARIDIVNVVAQTILLELQASGGRGYLKNDTSGFSRRWKEAAF
                     LPVVTPSAVQLRMVLANS"
     gene            complement(9837..10379)
                     /locus_tag="PXB27_000093"
     CDS             complement(9837..10379)
                     /locus_tag="PXB27_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDL5344930.1"
                     /translation="MSQFQIHTIESAPETAQEALKAVQQANGFIPNLIGVLANAPTAL
                     ETYRTVGGINSRNSLTAEEREVVQITAAVVNGCGFCVAGHTKIALKALKMPEEVVNAL
                     RATARIDDNKLDTLARFTLAVMLQKAKLTEAQLNEFFAAGYNQQNAIDVILGVSLATL
                     CNYVNNIAEIPINPELQPFA"
     gene            complement(10531..12042)
                     /gene="glpK"
                     /locus_tag="PXB27_000094"
     CDS             complement(10531..12042)
                     /gene="glpK"
                     /locus_tag="PXB27_000094"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDL5344931.1"
                     /translation="MEKQYIIALDQGTTSSRAVLLDKNANVVEVAQREFTQIYPQAGW
                     VEHNPMEIWSSQSSTLNEVVAKAGIKPDSIAAIGITNQRETTIVWEKETGKPVYNAIV
                     WQCRRTSDITDKLKADGHEEYIRKTTGLVVDPYFSGTKVKWILDNVEGAREKAERGEL
                     LFGTVDTWLVWKLTQGRVHVTDYTNASRTMLFNIHTKQWDDKMLELLNIPRSMLPEVK
                     NSSEVYGQTNIGGQGGVRIPVAGIAGDQQAALYGHLCVKEGQAKNTYGTGCFMLMHTG
                     NTAIQSENGLLTTIACNAKGEPEYALEGSVFIAGASIQWLRDELKIVHDSFDSEYFAT
                     KEDDSNGVYVVPAFTGLGAPYWDPYARGSIFGLTRGANRNHIVRATLESIAYQTRDVL
                     EAMQSDSKAKLQALRVDGGATANNFLMQFQADILDTKVERPKVKEVTALGAAYLAGIA
                     VGFWKDLEELKDKAEIERTFTPDGDQEKRAKRYKGWKKAVRRSLEWAKEDAEE"
     gene            complement(12046..12831)
                     /locus_tag="PXB27_000095"
     CDS             complement(12046..12831)
                     /locus_tag="PXB27_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDL5344932.1"
                     /translation="MSKSLRNACIGEFIGTGFILFFGAGCVAAAQVAGANFGLWEISI
                     VWGLGVSMAIYISAGISGAHLNPAVTIALAAFYGFEKHKILPYVIAQVAGAFCSVALI
                     YFMYSDLFTAAEAAQGITRGETVGFAGVFSTYPNPNITLVTAFIVEFVITVVLMSTIL
                     AIGDDKNGLPNKALAALLIGLLIAVIGGATGPLTGFAMNPARDFGPKLFAYLAGWGEI
                     ALTGGKEIPYFIIPIVAPICGALFGAWGYKNLIHNNLPANTQE"
     gene            13044..13403
                     /locus_tag="PXB27_000096"
     CDS             13044..13403
                     /locus_tag="PXB27_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HDL5344933.1"
                     /translation="MISIGAVLGAMCRWQLAVWLNPILSQFAFGTLAANWLGCLLIGI
                     AMGFNLGESQRLLFISGFLGSFTTFSSFSLELTEKLLAEKWGQFVTVLSLHLIGGLLL
                     TLIGVMLARAVTSGRFC"
     gene            complement(13451..14773)
                     /locus_tag="PXB27_000097"
     CDS             complement(13451..14773)
                     /locus_tag="PXB27_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HDL5344934.1"
                     /translation="MSAMFIIQFAIVLLCILVGARVGGIGLGVFGGLGLAILSFGFGL
                     KPAGLPIDVMFMIMAVVSAAAAMQAAGGLDYMIKIATRILRKNPKYITFIAPLVTWTF
                     TVLAGTGHVAYSVLPVIAEVSRHNGIRPERPLSMAVIASQFAIVASPIAAAVVAVVAF
                     LEPQGVTLGNVLSVTIPATLLGLGLACVFVNKMGKELKDDPNYQRLLQDPEYVKENHI
                     TTNPTELPLKPTAKLSVGLFLFGALLVVLMGAIPALRPVFDGKPMGMAHTIEIVMLSI
                     GALIIFFCKPDGNEITKGSVFHAGMRAVIAIFGIAWLGDTLMQGHMDEVKSMVSGLVE
                     TAPWAFAFALFVLSVLVNSQGATVATLFPVAIAIGIPAPVLIGVFVAVNGYFFIPNYG
                     PIIASIDFDTTGTTRIGKYIFNHSFMLPGLLSMIFSIGFGLLLSNILL"
     gene            14922..15746
                     /gene="dapF"
                     /locus_tag="PXB27_000098"
     CDS             14922..15746
                     /gene="dapF"
                     /locus_tag="PXB27_000098"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDL5344935.1"
                     /translation="MQFSKMHGLGNDFMVVDGVTQNVYLTEEMIRTLSDRHRGIGFDQ
                     LLLVEPPYDPELDFHYRIFNADGSEVSQCGNGARCFARFVTLKGLTNKKDIHVSTAKG
                     KMVLSLQEDGKVRVNMGEPIWEPSQVPFTANKFEKNYILRTELQTVLCGVVSMGNPHC
                     VLQVDDVKTAPVNELGPLLESHERFPERANISFMQVVNRNHVKLRVYERGAGETQACG
                     SGACGTVAVGIMQGLLDNKVQVDLPGGTLIIEWQGVGHPLYMTGDATHIYDGFIKL"
     gene            complement(15917..16459)
                     /locus_tag="PXB27_000099"
     CDS             complement(15917..16459)
                     /locus_tag="PXB27_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase family protein"
                     /protein_id="HDL5344936.1"
                     /translation="MAQVYIVHGYTANADKHWFPWLEQELAKCGVSCERLNMPDSAKP
                     SLDGWLNHLAQNVELTDQTVFVGHSLGCIAILNFLAKNYAKIKGAVFVSGFYQPVENL
                     PELSAFTNYYAILPSVSSFPSYVVSALDDKVVNHQYSDALAQHLHADYIRLNQGGHFL
                     DREGVTELPVVLELVKKLLE"
     gene            16583..16906
                     /locus_tag="PXB27_000100"
     CDS             16583..16906
                     /locus_tag="PXB27_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QacE family quaternary ammonium compound efflux
                     SMR transporter"
                     /protein_id="HDL5344937.1"
                     /translation="MNVWILLAISICLEIAATNLLKLSNGFTKAIPTIGSLALYGLSF
                     YFLSIIFRTLPVGIVYAIWSGVGIVLTALVAYFAFGQKIDLAGLIGISLIIAGVLVIN
                     LFSKV"
     gene            complement(16969..18414)
                     /gene="xylB"
                     /locus_tag="PXB27_000101"
     CDS             complement(16969..18414)
                     /gene="xylB"
                     /locus_tag="PXB27_000101"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HDL5344938.1"
                     /translation="MYIGIDLGTSGVKVVLLDENQHIIAITQKSLPIFRPHPLWSEQN
                     PQDWWNATNEAMLELSATQDLQAVKAIGLTGQMHGATLLDNADNVLSPAILWNDGRSF
                     MECEELEQLVPNSREITGNLMMPGFTAPKLRWVDKHQPDIAEKVSKVLLPKDYLRLLM
                     SGEYASDMSDASGTMWLDVGKRNWNKSLLNACGLDIENMPKLFEGNQITGYLRPTLAE
                     QWKMKSVPIVAGGGDNAAGAIGIGLYQTGQAMLSLGTSGVYFVVSDKFQANPQKAVHS
                     FCHALPDRWHLMSVILSAASAVDWAVKSLGLKDVSTLFQQVEASQTASDAIFLPYLSG
                     ERTPHNDPYAKGVFWGLSHNDNQITMAKAVIEGVSFALAEGIEVLHETGVVADNIALI
                     GGGAKSVYWRQLLADISGKTFEYRTSGDVGPALGAAKLAQIALNPNQDIATFCQPLPL
                     EQVYQPTPARYAEYQQKRQKFTELYRRLKGL"
     gene            complement(18567..19886)
                     /gene="xylA"
                     /locus_tag="PXB27_000102"
     CDS             complement(18567..19886)
                     /gene="xylA"
                     /locus_tag="PXB27_000102"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012072255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HDL5344939.1"
                     /translation="MSNYFDKISKVQYEGANSTNPFAFKHYNPDEVVLGKTMAEHLRL
                     AVCYWHTFCWTGNDMFGVGSLDRSWQKTGDLLQGAKQKAEIAFEFFQKLGIPYYCFHD
                     VDVAPEGNNFKEYQHNFNTIVDILEQKQAETGVKLLWGTANCFTNPRYMSGASTNPNP
                     EVFAWAAAQVFTAMNATKRLGGENYVLWGGREGYETLLNTDLKREREQIGRFMQLVVE
                     HKHKIGFNGTLLIEPKPQEPTKHQYDYDVATVYGFLKQFGLEKEIKVNIEANHATLAG
                     HTFQHEIATATALDIFGSIDANRGDPQLGWDTDQFPNSVEENTLVMYEILKAGGFTTG
                     GFNFDAKIRRQSTDPYDLFHAHIGAIDVLALSLKRAAKMLEDQALQKVVDNRYAGWNQ
                     DLGLQILNGKASLEDLAKIVETQGLDPKPVSGQQEYLENLVNSYIYR"
     gene            20128..21129
                     /gene="xylF"
                     /locus_tag="PXB27_000103"
     CDS             20128..21129
                     /gene="xylF"
                     /locus_tag="PXB27_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005576719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL5344940.1"
                     /translation="MKLKSKLLAVATATLMVLSNASVAKDLKIGMSIDDLRLERWQKD
                     RDIFVNKAESLGAKVFVQSANGDATAQISQIENMLNKDIDVLVIIPFNGEVLSNVIAE
                     AKKEGVKVLAYDRLINNADVDFYVSFDNEKVGELQAKSIIGQKPEGNYFLMGGSPVDN
                     NAKLFRKGQMKVLQPLIDSGKIKVVGDQWVDSWLAEKALQIMENALTANKNNIDAVVA
                     SNDATAGGAIQALSAQGLSSKVAISGQDADLAAIKRIIAGTQTMTVYKPITNLADKAA
                     EIAVALGKDEKVEMNAQLNNGMKEVPAYLLEPVVVTKENIDDTVIKDGFHTKDAVYK"
     gene            21196..22710
                     /gene="xylG"
                     /locus_tag="PXB27_000104"
     CDS             21196..22710
                     /gene="xylG"
                     /locus_tag="PXB27_000104"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HDL5344941.1"
                     /translation="MAKLLEMKNITKKFGEVVALNNISISLETGEILSLCGENGSGKS
                     TLMKVLCGIYPAGDYEGEIYFSGEKLIARNIKDTEEKGISIIHQELTLVKNMSILENM
                     FLGNEITKSGITDDNEMYLRCKTLLEQVQLDIDPNTKVGELGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEKETEILLSLIKDLQAHNIACVYISHKLNEVKAISDKICVIRDG
                     EHIGTRSAEGMSEDDIITMMVGREITSLYPHEPHNIGEEVLRVENLTAWHPTNTHIKR
                     VDNANFVLRQGEILGIAGLVGSGRTEMAQCIFGSYQGKYQGDIFLHKQPIKIKNCAQA
                     IENHIVMVPEDRKKHGIIPIMGVGKNITLSSLPQFCFGKKVINEPLEETIINQSIAKL
                     KVKTSSPELAIGRLSGGNQQKAILAKCLLLNPKILILDEPTRGIDVGAKYEIYKLINQ
                     LAKEGMAIIVISSELPEVLGISDRVLVMHQGKIKADLINQDLTQEKVMEAALKE"
     gene            22714..23841
                     /locus_tag="PXB27_000105"
     CDS             22714..23841
                     /locus_tag="PXB27_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL5344942.1"
                     /translation="MNKLKSINLQVYIMLIAIVVIMSFFSIATDGAYLSARNISNLLR
                     QTSITGILAIGMVFVIISAEIDLSVGSLMGLLGGFAAISNVWWGWPLSLTIIVTLILG
                     VMLGAWSGWWVAYQKVPSFIVTLAGMLAFRGVLVGLTNGTTVSPISNEMTTIGQGYLP
                     DMVGMILGTIGIFCFIVWGSYQRKARQKLNLTVPSMSKDTLKYGLVAIIVLGAIYLLN
                     DYRGVPFPVLVLVILAIAGTFLARKTAFGRHIYAIGGNIDAARLSGISVEKVKLAIFA
                     LNGLLVAIAGLILSSRLGAGAPSAGQNAELDAIAACVIGGASLAGGIGSIYGVVIGAF
                     IIALLDNGMSMLDVPTFWQYIVKGGILLLAVWVDTISKKKA"
     gene            complement(23857..24387)
                     /locus_tag="PXB27_000106"
     CDS             complement(23857..24387)
                     /locus_tag="PXB27_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL5344943.1"
                     /translation="MSENSLKTHYSAKELAELSLACLPKTKQGVLYQAKKQGWTTHKK
                     VGVGGEILEFALSNLPQEIQTEIRSRFMSAVVEAKPKKLPAVRAEVDVGNLTTKQREI
                     ADARMALVAYVLELEGSMSRIKAITYLCNLAKQGEMPPHLVELVAVANAKKTAKRTIV
                     PIFSPIMPDFPLDSRD"
     gene            complement(24403..24666)
                     /locus_tag="PXB27_000107"
     CDS             complement(24403..24666)
                     /locus_tag="PXB27_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL5344944.1"
                     /translation="MSTLEDIKKTAKDWHRADIIAALHKAGWSLRQLSLKHGYSQSST
                     LKNAVDRPWLKGERIIAEAIGISPEEIWPERYAQRNHKKFADR"
     gene            24850..25332
                     /locus_tag="PXB27_000108"
     CDS             24850..25332
                     /locus_tag="PXB27_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5344945.1"
                     /translation="MKIECKKVIGFGNRIEQSRKNAKLSRSEVCDALGGIAISTLQAW
                     EADTREPPISKLMALSQILNVEPCYLLTGERHSQSLNVTREPQPVYATKPQTEEGEVV
                     VIKAKLQEAIETLEEVLEITQREMKPAGKAQMVWAFYELLTQEKSEKEKMVGLLKLVA
                     "
     gene            25344..25595
                     /locus_tag="PXB27_000109"
     CDS             25344..25595
                     /locus_tag="PXB27_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5344946.1"
                     /translation="MDKQAILKLFRDNSTAGNSQPSQEPIQVFNNSNVIIQNGDNATV
                     IAGCTVDNNTNLTCADKQRVHDLIHSLEQRNRASRDEGA"
     gene            complement(25885..27750)
                     /locus_tag="PXB27_000110"
     CDS             complement(25885..27750)
                     /locus_tag="PXB27_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HDL5344947.1"
                     /translation="MQSLLSFVPDFLKQELFWVMALLVFAIWQFIQNKLRMDVVALIV
                     MLFFSLTNILSVKEVLAGLSDPNVVLIALLFIVGEGLVRTGVANQVSDWLMRVSGASE
                     TKVLVLLMLCIAGLGSFMSSTGIVAIFIPVVLAICSGMGISPRRLMMPLSVAGLISGM
                     MTLIATPPNLVAHSELVKSGFEGFNFFSFTPIGLIILVMGIGYMLIARRWLDNGEQSN
                     THSQAQKFSMSQLIEEYQLKERAKMVLVEADSDCVGKSIGQLNLRIEQGLNIIAIQRT
                     KRFRKITVSASVNEIFQPKDILLLDTALDDETYQQRCEQLKLRPIELKGEYFSSQYRS
                     VGMAEISVMPEAECIGKTIRELKFRSRYHLSIVGLKRGDEIIQEELLDTPLKFGDILL
                     VMGVWQQIHRMDDDHRDFFLLRDPAESKNAPPALSQAPHALFSVLTMVLLMITGIVPN
                     VMAALIACLMLGKFRCVDMKSAYDSIHMPSIVLIVGMMPFSIALQKTGGVDLTVRLLL
                     DAMQGLDVHFVLIVLFVVTAIISAFISNTATAILVMPIAIAIANQLGYSPAPFVMTVA
                     VSASAAFMTPVSSPVNTMVLAPAGYKFIDFVKVGVPFTVLVMLVSVFVIPMLFPL"
     gene            complement(27890..28405)
                     /locus_tag="PXB27_000111"
     CDS             complement(27890..28405)
                     /locus_tag="PXB27_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MltR family transcriptional regulator"
                     /protein_id="HDL5344948.1"
                     /translation="MMDYIDKLSEETTLRGFLSIANQRFAKAVEQLIERVFRKTDFAL
                     KSVVDSLFEHQGPLADIEVRLKVLLGLGVISPKVFEDISLFLEVKQHLNEEEDEFAFS
                     HPAVVKFAKDLHHIDFLPINELLNMKASAANKDSMLFQMQQMRLEKMVRSSLILAVTE
                     IDEQLNVESPL"
     gene            complement(28417..29556)
                     /locus_tag="PXB27_000112"
     CDS             complement(28417..29556)
                     /locus_tag="PXB27_000112"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005557909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDL5344949.1"
                     /translation="MNALHFGAGNIGRGFIGKLLADARIFVTFADINQTQIDQINQNK
                     QYGVKIVGDDSRVEIVKNIAAINSKDENAVIEQVKNTDLITTAVGPNVLGFIAPLFAK
                     ALVARVESGNTQPLNIIACENMVRGTTFFKGKIFEHLTAEQQAEIEKVVGFVDSAVDR
                     IVPPAEPNPADPLEVTVEEFSEWIVDQTQFKGDIPNIKGMELTDNLMAFVERKLFTLN
                     TGHLICAYLGKQAGVKWIKEAIAIEEIKTQVKATMEESGAVLIKRYGFDPQAHSAYIE
                     KILKRFANPYLNDDVNRVGREPIRKLSENDRLIKPLRGTLEYGLPYQNLVKGVVMALQ
                     YRNEEDPQAVELAEFIANHGVAAAVEKYTGLTDQAVIAQVVELYQ"
     gene            complement(29660..31519)
                     /locus_tag="PXB27_000113"
     CDS             complement(29660..31519)
                     /locus_tag="PXB27_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HDL5344950.1"
                     /translation="MSNLKVKVQGFGRFLSNMVMPNIGAFIAWGFITALFIPTGWLPN
                     ETFAKLVGPMITYLLPLLIGYSGGKLVGGERGAVVGAITTMGIIVGSEIPMFLGAMIV
                     GPLGGWAIKTFDKAIDGKVKSGFEMLVNNFSVGIIGMILAMLSFAFIGGIVTQISAAL
                     GAGVGALVEAGLLPVTSIIVEPAKILFLNNAINHGVFTPLGTAQAQEVGKSILFLIEA
                     NPGPGFGILLAYMFFGRGTAKQTAGSASIIHFLGGIHEIYFPYVLMSPRLILAVIAGG
                     ATGVLTNSLLGGGLIAPASPGSIFAILAMTAPGAHFAVVASVILSCAVTFAIASILLK
                     TQKGEASLEDAQAQTQAMKAESKGVVNGNINKIYVACDAGMGSSAMGASMLRKKVQAA
                     GLNIEVQNLAINDLPQDAQLVITHKDLTARATQRVATAQHLSLTNFLDSAFYDSLVAK
                     LANHQPQQAVQNTQNFANPVEEPQAEGLKLQAAQVFLGLQANSKEEAIRFAGEQLVKA
                     GFVQPSYVDAMFAREQMVSTYLGEGLAVPHGTSDAKDSVLKTGIVVCQYPNGVRFTDE
                     EDGVAKLVVGIAAKGNDHIQILSAITNALDDEQAMKTLTSTNDVEKVLALLSK"
     gene            31821..32371
                     /locus_tag="PXB27_000114"
                     /pseudo
     CDS             31821..32371
                     /locus_tag="PXB27_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005752768.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(32415..33350)
                     /locus_tag="PXB27_000115"
     CDS             complement(32415..33350)
                     /locus_tag="PXB27_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HDL5344951.1"
                     /translation="MNHLDSAKETLNLYAQQIITLNHRLSEEFNEVVNMILACQGRVV
                     VGGIGKSGLVGKKMVATFASTGTPSFFLHPTEAFHGDLGMLKPIDMVILISNSGETDD
                     VNKLIPSLKNFGNKIIAMTGNPYSTLGRNADVILNIGVEREACLNNLAPTSSTLVTMA
                     LGDALAIALMKARDFRPEDFARYHPGGSLGRKLLNRVRDVMVRKVPTASLDTTFTECL
                     SVMNEGRMGVAVIMQDDKLEGIITDGDIRRTLAKFGAESLNKTADEIMTRNPKTINDT
                     EFLAKAEDLMKELKIHSLIAVNDEGKVTGLMEFSS"
     gene            complement(33361..34158)
                     /locus_tag="PXB27_000116"
     CDS             complement(33361..34158)
                     /locus_tag="PXB27_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005725056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor"
                     /protein_id="HDL5344952.1"
                     /translation="MKPFERRQQILAYLAQHGRTEVDVLADYFKLTGATIRKDLTSLE
                     EDKKVLRTYGSVVLIDEEILEREEKINKEDKDDKPLAQKTGINLQQKQAIGQKAAELI
                     NEGNSIIFDAGSTVLQMIPHLKAFDNLSLMTNSLTIFNEILKLNKSYNLLISGGTFRE
                     KSASFHGYFAESAFIGSNFDILFIGTDGLDLDVGLTTFNEVYKVSSLMCDAANKIVLL
                     ADSSKFGRKSPNIVCGLEKIHTIITDEGLSQEMREQIKAKGIELLVV"
     gene            complement(34201..34848)
                     /gene="hxpB"
                     /locus_tag="PXB27_000117"
     CDS             complement(34201..34848)
                     /gene="hxpB"
                     /locus_tag="PXB27_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HDL5344953.1"
                     /translation="MQINSVIFDMDGVMIDSEPQWAEAQIAVLADLGINITEQDCEQL
                     TRGKRIDELSDTWIKKFQLSVSNQALSEAILAHGCAAIEQHGKALTGLYELLDFLKSN
                     QIKLAVATSSPPVVIKAVFDRLKLWDYFPIRCSAMDEEYGKPHPAVYLSAVKKLGIEK
                     QECIVIEDSVTGLIAAKAANLTTFLVNTDYQNEKFSIADKRMASLLDVIEKLKQH"
     gene            complement(34911..35291)
                     /gene="gutM"
                     /locus_tag="PXB27_000118"
     CDS             complement(34911..35291)
                     /gene="gutM"
                     /locus_tag="PXB27_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HDL5344954.1"
                     /translation="MDSITLLIILAMIMWLLQAVLGWLQIKAFNQAFMEISKKGKVTV
                     GRNSGRFNPKSVIVLAFDDEKKVVDSLCMKGFSVFARPQKLASVIGLSLDQIVPEKIF
                     PSDKRSQFALKVAISSGYSCSDQK"
     gene            complement(35460..36248)
                     /gene="srlD"
                     /locus_tag="PXB27_000119"
     CDS             complement(35460..36248)
                     /gene="srlD"
                     /locus_tag="PXB27_000119"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HDL5344955.1"
                     /translation="MNNEKKVAIVVGGGQTLGAFLSEGLADAGYRVVVADLNGENAQK
                     VAADINAKQGEGNAIGVQVDAADEPSVEALAKATDTVFGRADVLVYSAGTAKASPIWD
                     FNLNDFDLSVKVNLTGYFLTAKHFSRLMIRDGIKGRIIQINSKSGKVGSKYNSGYSAA
                     KFGGVGLTQSLALDLADKGITVHSLMLGNLLKSPMFQSLIPQYAAKLGIPESEVEQVY
                     IDKVPLKRGCDYQDVLNVLKFYAGEEAAYCTGQSINITGGQVMF"
     gene            complement(36296..36694)
                     /gene="srlB"
                     /locus_tag="PXB27_000120"
                     /gene_synonym="gutB"
     CDS             complement(36296..36694)
                     /gene="srlB"
                     /locus_tag="PXB27_000120"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDL5344956.1"
                     /translation="MHRLSKRENIYMTIIYQTTFTKIGNFAQESLTDDMLITFKQGAP
                     ADLQDYCFIHNPSELSSPLEVGDIAEFDGVAYPITAVGSVASENLSALGHITFRFDGA
                     NDAEFPGSVHVIGTPPQGLTENSTLIIKRD"
     gene            complement(36739..37740)
                     /locus_tag="PXB27_000121"
     CDS             complement(36739..37740)
                     /locus_tag="PXB27_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDL5344957.1"
                     /translation="MSNKAIYIEKGNGGWGGPLTLPVVEGKKVLYMTGGTRPAIVNKL
                     VELTGWEAVDGFKEGEPPAEEIGIAIIDCGGVLRCGLYPKRRIPTINIHSTGKSGPLA
                     EFIVEDIYVSAVKENNIRLIDADGSVPVATEQAKPTSSTTATAKEYDSSKKITEQSDG
                     LLAKIGMGMGSVAALFFQAGRDTIDTVLKTILPFMAFVSALIGIIIASGLGDVIAHGL
                     TPLANSPIGLVTLALICSFPLLSPFLGPGAVIAQVIGVLVGTQIGLGNIPPHLALPAL
                     FAINAQAACDFIPVGLSMAEAKQDTVRVGVPSVLVSRFLTGAPTVLIAWAVSAFIYQ"
     gene            complement(37771..38325)
                     /gene="srlA"
                     /locus_tag="PXB27_000122"
     CDS             complement(37771..38325)
                     /gene="srlA"
                     /locus_tag="PXB27_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005725059.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDL5344958.1"
                     /translation="MIEAITHGAEWFIGLFQKGGETLVGMINGILPLLISLLVVMNAL
                     IKFVGQDRIERLAQRSAGNPISRYLILPVIGTFVFCNPMTLSLGRFLPERYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLGLPLGPLAISYLLVGMFTNFFRGWI
                     TDFTTAFFEKRMGITLDREVHLSH"
     gene            complement(38563..38871)
                     /locus_tag="PXB27_000123"
     CDS             complement(38563..38871)
                     /locus_tag="PXB27_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HDL5344959.1"
                     /translation="MKKYIATMLLAFGLVHSSYAQEKTAQIETKQAEKTVVLHIPEMY
                     CQLCVYLVNKELRAVEGVISTKASMKERKATVVVKESVDNQALIQAVGNLKYTAKVIE
                     "
     gene            complement(38873..39241)
                     /locus_tag="PXB27_000124"
     CDS             complement(38873..39241)
                     /locus_tag="PXB27_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5344960.1"
                     /translation="MNSSLKSYNHKFITMCVTAIVSAVTSTFCCIGPLVYLVFGISSP
                     WLMELNQLEFLQLPMLLISLAAFGYGFWLLNFSNKIICTKYFSRRTLVILYWVVSVVV
                     MFFLTYPYVLPYILEMWSGE"
     gene            complement(39216..40322)
                     /locus_tag="PXB27_000125"
     CDS             complement(39216..40322)
                     /locus_tag="PXB27_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HDL5344961.1"
                     /translation="MNKLIASLVASSCATAFAAPVQQNNFHTTTHTYEFTQSYDLVVP
                     QGSKGTTNLWIPLPSDTDYQTVKSIEFEGSYKNAYITENNAYGAKTLFATWDTDAPKR
                     DLKVKMVIQTQDREPMAQGALANYQMPEKITYSVDVLEYLKPTAHIKTDGIVKEYADK
                     IVGNEQNPLKKAELIHKWIVDNMERDNSVLGCGDGDVEKILTTKVLKGKCTDINSVFV
                     ALARASNIPAREVFGIRLGQAVKMGQYSKTAFGSSKDGVANESGGQHCRAEFYLAGFG
                     WVPVDSADVAKMRLAEKKDVDDADVQAVSKYLFGNWEMNWVGFNHGRDFDLYPQPELT
                     PLNNFGYPYAEVGGDPLNSFEAKEFGYEFISKEL"
     gene            complement(40562..41605)
                     /locus_tag="PXB27_000126"
     CDS             complement(40562..41605)
                     /locus_tag="PXB27_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL5344962.1"
                     /translation="MQPINIAIAGAGFSSRVFHIPFLKQDARFNIVKVYERTSNNAAE
                     WLPNAETVRDFNALLTPEVDLVLITTPNQTHYAMVKESLLAGKHVLVEKPLVATVAEA
                     EELAELAKRQGKVLYVYQNRRWDSHIATAKSLMEQNLLGELVDCEIRFDRYAKEKNVK
                     VWKESGELGTGLVYDLGVHLIDQALYLFGKPEAVYADVRYQHEGALVDDNFDIQLYYA
                     NGFKVVLKASRYAREPSPTFVLHGKLGSYLKQTPDNQEDLLKNGVQPIGKDWNIEEKE
                     NWGILHTEVEGNVVRQPYRNAEVSYQNLFDDLYQAIAHNAEPIIKLEDVIFVLKVIES
                     VFESAKLGQKIYL"
     gene            complement(41709..41993)
                     /locus_tag="PXB27_000127"
     CDS             complement(41709..41993)
                     /locus_tag="PXB27_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5344963.1"
                     /translation="MRYWLISLSILLSGCVVPVTPTGQAGIPMPIIIGVSDPLDSKEV
                     HICTLKPFTQEYRSEHSSRGRAKLNVQKQCRANHSEMFCEEKDIVCKTYD"
     gene            complement(42023..42208)
                     /locus_tag="PXB27_000128"
     CDS             complement(42023..42208)
                     /locus_tag="PXB27_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HDL5344964.1"
                     /translation="MKCRQATELISQSCDEKLRLQQHMRLQLHLLVCPKCRNFNKNTK
                     KLGKILRAYCNDKDAPF"
     gene            complement(42205..42771)
                     /locus_tag="PXB27_000129"
     CDS             complement(42205..42771)
                     /locus_tag="PXB27_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL5344965.1"
                     /translation="MKSLSSTQIEEIRKQMVKFATLQLADKDLAEDVVQDALTNAYKH
                     SASFRREAALKTWIFAILKNKILDLLRTRKRHIPVSSLCEEEESPSAFFDRHDHWLEH
                     QDVTEWRGVAQSTYQKEFWAIFDICLNKLPAQQARVFMMREHLEMSSAEICQECEISL
                     SNLNVLLHRARLRLQECLSRNWFREESK"
     gene            42880..43833
                     /gene="msrP"
                     /locus_tag="PXB27_000130"
     CDS             42880..43833
                     /gene="msrP"
                     /locus_tag="PXB27_000130"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HDL5344966.1"
                     /translation="MKKLTIQDVTPETIFYQRRKIITALGLGIAASALPKVSMAETQR
                     HLQSLQFKPDPNKGLELTPENKVIGYNNFYEFGVDKSAPAKNAANFKTDPWTLEIGGE
                     VENPFSLNHQQLLNTFPLEERIYRFRCVEAWSMVVPWIGFELARLIEMAKPTSKAKYV
                     VFHTLYDPAQMPGQKNRLLGGGIDYPYVEALRLDEALNSLTLLSVGLYGKTLPPQNGA
                     PIRLVVPWKYGFKSIKSIVKITFAEKRPLTTWESLAPNEYGFYANVNPNVDHPRWSQA
                     SERVIGAGGLLSVKRQPTLMFNGYENEVANLYRGLDLKVNY"
     gene            43833..44417
                     /locus_tag="PXB27_000131"
     CDS             43833..44417
                     /locus_tag="PXB27_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoxide reductase heme-binding subunit YedZ"
                     /protein_id="HDL5344967.1"
                     /translation="MLTWLRILIHLGCLLPLVWVAQLLYTGNETILGADPIKELEHFL
                     GYGAIVIFCIMFLLGIALQYLKKNQYQILRRPLGLWAFVWAALHISSYLLLELSLDVK
                     LFFSELAGRPYLILGAAAFFILSIMAITSLPMLKRKLGTRWGTIHQWAYPALVLAMVH
                     YYWSVKSVTFDPIIIGVLVLFIVAHKIQAKFAKK"
BASE COUNT        13132 a         9373 c         9135 g        12849 t
ORIGIN      
        1 ctagacttac aaccctatca aatgggctaa aaaacacttt aacggcaacc aaaagtggct
       61 tacataaagc cggtcaatca ttaacccaag ccggcagttc tttaaaaact ggggcaaaaa
      121 aaattatcct ctatattccc caaaattacc aatatgatac tgaacaaggt aatggtttac
      181 aggatttagt caaagcggcc gaagagttgg ggattgaggt acaaagagaa gaacgcaata
      241 atattgcaac agctcaaacc agtttaggca cgattcaaac cgctattggc ttaactgagc
      301 gtggcattgt gttatccgct ccacaaattg ataaattgct acagaaaact aaagcaggcc
      361 aagcattagg ttctgccgaa agcattgtac aaaatgcaaa taaagccaaa actgtattat
      421 ctggcattca atctatttta ggctcagtat tggctggaat ggatttagat gaggccttac
      481 agaataacag caaccaacat gctcttgcta aagctggctt ggagctaaca aattcattaa
      541 ttgaaaatat tgctaattca gtaaaaacac ttgacgaatt tggtgagcaa attagtcaat
      601 ttggttcaaa actacaaaat atcaaaggct tagggacttt aggagacaaa ctcaaaaata
      661 tcggtggact tgataaagct ggccttggtt tagatgttat ctcagggcta ttatcgggcg
      721 caacagctgc acttgtactt gcagataaaa atgcttcaac agctaaaaaa gtgggtgcgg
      781 gttttgaatt ggcaaaccaa gttgttggta atattaccaa agccgtttct tcttacattt
      841 tagcccaacg tgttgcagca ggtttatctt caactgggcc tgtggctgct ttaattgctt
      901 ctactgtttc tcttgcgatt agcccattag catttgccgg tattgccgat aaatttaatc
      961 atgcaaaaag tttagagagt tatgccgaac gctttaaaaa attaggctat gacggagata
     1021 atttattagc agaatatcag cggggaacag ggactattga tgcatcggtt actgcaatta
     1081 ataccgcatt ggccgctatt gctggtggtg tgtctgctgc tgcagccggc tcggttattg
     1141 cttcaccgat tgccttatta gtatctggga ttaccggtgt aatttctacg attctgcaat
     1201 attctaaaca agcaatgttt gagcacgttg caaataaaat tcataacaaa attgtagaat
     1261 gggaaaaaaa taatcacggt aagaactact ttgaaaatgg ttacgatgcc cgttatcttg
     1321 cgaatttaca agataatatg aaattcttac tgaacttaaa caaagagtta caggcagaac
     1381 gtgtcatcgc tattactcag cagcaatggg ataacaacat tggtgattta gctggtatta
     1441 gccgtttagg tgaaaaagtc cttagtggta aagcctatgt ggatgcgttt gaagaaggca
     1501 aacacattaa agccgataaa ttagtacagt tggattcggc aaacggtatt attgatgtga
     1561 gtaattcggg taaagcgaaa actcagcata tcttattcag aacgccatta ttgacgccgg
     1621 gaacagagca tcgtgaacgc gtacaaacag gtaaatatga atatattacc aagctcaata
     1681 ttaaccgtgt agatagctgg aaaattacag atggtgcagc aagttctacc tttgatttaa
     1741 ctaacgttgt tcagcgtatt ggtattgaat tagacaatgc tggaaatgta actaaaacca
     1801 aagaaacaaa aattattgcc aaacttggtg aaggtgatga caacgtattt gttggttctg
     1861 gtacgacgga aattgatggc ggtgaaggtt acgaccgagt tcactatagc cgtggaaact
     1921 atggtgcttt aactattgat gcaaccaaag agaccgagca aggtagttat accgtaaatc
     1981 gtttcgtaga aaccggtaaa gcactacacg aagtgacttc aacccatacc gcattagtgg
     2041 gcaaccgtga agaaaaaata gaatatcgtc atagcaataa ccagcaccat gccggttatt
     2101 acaccaaaga taccttgaaa gctgttgaag aaattatcgg tacatcacat aacgatatct
     2161 ttaaaggtag taagttcaat gatgccttta acggtggtga tggtgtcgat actattgacg
     2221 gtaacgacgg caatgaccgc ttatttggtg gtaaaggcga tgatattctc gatggtggaa
     2281 atggtgatga ttttatcgat ggcggtaaag gcaacgacct attacacggt ggcaagggcg
     2341 atgatatttt cgttcaccgt aaaggcgatg gtaatgatat tattaccgat tctgacggca
     2401 atgataaatt atcattctct gattcgaact taaaagattt aacatttgaa aaagttaaac
     2461 ataatcttgt catcacgaat agcaaaaaag agaaagtgac cattcaaaac tggttccgag
     2521 aggctgattt tgctaaagaa gtgcctaatt ataaagcaac taaagatgag aaaatcgaag
     2581 aaatcatcgg tcaaaatggc gagcggatca cctcaaagca agttgatgat cttatcgcaa
     2641 aaggtaacgg caaaattacc caagatgagc tatcaaaagt tgttgataac tatgaattgc
     2701 tcaaacatag caaaaatgtg acaaacagct tagataagtt aatctcatct gtaagtgcat
     2761 ttacctcgtc taatgattcg agaaatgtat tagtggctcc aacttcaatg ttggatcaaa
     2821 gtttatcttc tcttcaattt gctagagcag cttaattttt aatgattggc aactctatat
     2881 tgtttcacac attatagagt tgccgtttta ttttataaaa ggagacaata tggaagctaa
     2941 ccatcaaagg aatgatcttg gtttagttgc cctcactatg ttggcacaat accataatat
     3001 ttcgcttaat ccggaagaaa taaaacataa atttgatctt gacggaaaag ggctttcttt
     3061 aactgcttgg cttttagctg caaaatcgtt agcgttgaaa gcgaaacaca ttaaaaaaga
     3121 gatttcccgc ttacacttgg tgaatttacc ggcattagtt tggcaagata acggtaaaca
     3181 ttttttattg gtaaaagtgg ataccgataa taaccgctat ttaacttaca atttggaaca
     3241 agatgctcca caaattctgt cacaagacga atttgaagcc tgctatcaag ggcagttaat
     3301 tttggtcacg tccagagctt ccgtagtagg tcaattagca aagttcgatt tcacctggtt
     3361 tattccggcg gtgatcaaat accgaaaaat ctttctagaa accttgattg tttcgatctt
     3421 tttgcaaatt tttgccctaa ttacaccgct attcttccaa gttgttatgg ataaagtact
     3481 ggtgcatcga ggtttttcaa ccttgaatat cattacggtt gccttagcta ttgtgatcat
     3541 ctttgaaatt gtactaagtg gtttgagaac ctatgttttt tctcatagca ctagccgtat
     3601 tgatgttgaa ttaggcgcta aattatttcg acatttatta tcactaccca tttcttattt
     3661 tgaaaacaga cgagttggag atacagtcgc tagggttaga gaattagatc aaattcgtaa
     3721 tttccttacc ggacaagcat taacctcggt gttagatctc ttattctctt ttatcttttt
     3781 tgccgtaatg tggtattaca gcccaaaatt aaccttggta attcttggtt cattgccctg
     3841 ctatatttta tggtcaattt ttattagtcc gattttaaga cggcgtttag atgagaaatt
     3901 tgcccgaagt gctgataacc aagcattctt agttgagtcg gtaacagcca tcaatatgat
     3961 taaagcgatg gcggttgctc cacaaatgac ggatacatgg gataaacagc tggcaagcta
     4021 tgtttcatca agtttccgtg tcaccgtatt agcaaccatt gggcaacaag gtgtacaact
     4081 tattcaaaaa accgttatgg tgattaacct ttggttaggg gcacacttag ttatttcagg
     4141 cgatctgagt attgggcaat taattgcctt taatatgcta tcagggcaag tgattgcacc
     4201 ggtgattcgg ctggctcagc tctggcaaga tttccaacaa gttgggattt ccgtcactcg
     4261 cttaggtgat gttttaaact ctccaaccga acaatatcaa ggcaaattat cactaccaga
     4321 aataaaaggc gatatctcat ttaaaaatat ccgctttaga tataaaccag atgcaccaac
     4381 tattttaaat aatgtgaatt tagaaattag gcaaggagaa gtgattggga ttgttggacg
     4441 ttccggttca ggcaaaagta ctctgactaa attactgcaa cgtttttata ttcctgaaaa
     4501 tgggcaggtt ttgattgatg gacatgatct agccttagct gatccaaact ggctacgccg
     4561 tcaaataggt gtagtgctgc aagataatgt gttattaaac cgcagtatcc gagaaaatat
     4621 tgcgctatca gatccaggaa tgccaatgga gcgagtaatt tatgcagcaa aattagcagg
     4681 ggctcacgat tttatttcag aattgcgtga aggttataac accattgtgg gtgaacaagg
     4741 agcggggctt tcaggcgggc aacgccaacg gattgcgatt gctcgagctt tggtaaacaa
     4801 cccgaaaatc ctgatttttg atgaggcaac cagtgccctc gattacgaat ctgagcatat
     4861 tattatgcaa aatatgcaaa aaatatgcca aggcagaacc gtgattttga ttgcacatcg
     4921 tttatcgacc gtcaaaaatg cggatcgaat tattgtgatg gaaaaggggg aaattgttga
     4981 gcaaggcaag caccacgaat tactgcaaaa cagtaacgga ctttattcct acttacacca
     5041 attacaactt aattaagaag gaaaacaatg aaaatatggc ttagtggtat ttatgaattt
     5101 ttcctacgct ataaaaacat ttgggcagaa gtatggaaaa ttcgtaaaga attagaccac
     5161 ccaaacagaa aaaaagacga aagtgaattt ttaccggcac atttagaact gattgaaacc
     5221 ccggtttcta aaaaaccacg tctaattgct tatttgatta tgctattttt agttgtggca
     5281 attgtgcttg ccagtgtaag caaagttgaa attgtggcga ctgctcccgg taaattaact
     5341 tttagtggca gaagtaaaga aattaaaccg attgaaaacg ccattgtaca agaaattttc
     5401 gttaaagatg ggcagtttgt ggaaaaaggg caattattag tcagcttaac tgcattgggt
     5461 tctgatgcag atatcaaaaa gaccatggct tcactttctt tagctaaact ggagaactat
     5521 cgctaccaaa ctttgcttac tgccattgaa aaagagtcct tgccggtgat tgatttatct
     5581 agaaccgaat ttaaagattc atcggaagaa gatcgactac gtattaaaca cttaattgag
     5641 gagcaataca ccacttggca aaaacaaaaa acacagaaaa ctttagcgta taagcgtaaa
     5701 gaggctgaaa aacaaacaat atttgcctat gtccgtaaat atgaaggtgc aacacgtatt
     5761 gaacaagaaa aattaaaaga ctttaaggca ctttataaac agaagtcttt atctaagcac
     5821 gaacttcttg cgcaagaaaa taaattaatt gaggctcaga atgagctagc tgtttatcgc
     5881 tcaaaattaa atgaattaga aaatgatcta ctcaatgtaa aagaagaact tgaattgatc
     5941 acgcaattct ttaaaagcga tgtgttggaa aaattaaagc aacatattga aaatgaacgc
     6001 caacttcggc tcgagttaga aaaaaataat caacgcagac aggcctcgat gatcagagca
     6061 ccggtttccg gtacggttca gcaactgaaa attcacacta taggtggtgt tgttacgact
     6121 gctgaaacct tgatgatcat tgtgccggaa gacgatgtgt tagaggccac cgctctggtt
     6181 ccaaacaaag atatcggctt tgttgcagca gggcaggagg tgattattaa agtggaaact
     6241 ttcccttata cacgctatgg ttatctaact ggtcgaatta aacatattag cccggatgcg
     6301 attgaacaac ctaatgtagg cttagttttt aatgcaacta tagctataga taggaagaat
     6361 ctaacatcgc ctgatgggcg aaaaattgat ttgagttcag gtatgacaat aactgctgaa
     6421 atcaaaaccg gtgaacggag tgtaatgagt tatttactca gcccattaga agaatctgtc
     6481 acagaaagtt taagggaacg ctaatcgaac caaaacaaag ccataaaagc cattttgagc
     6541 ttttatggct ttatttttta gtccacaagc ggtcaaaaaa gcccaatttt ttacactttt
     6601 ataacaaatt gttctaacta aaaattacta attcttttct tttataagac gaatcctcta
     6661 ttttcatttc attaacattg aactagaaaa ggggatttat gagcctaagc attacgaatc
     6721 tttctcttgg ctaccgcaaa aatcagcaaa ggcttatttg aaaagcacgg tgtgcaggtg
     6781 gaaaaaccgg tgatgtttcg cagctgggct cagttggtgg aagccttttt aagtggcaat
     6841 gtgaacgtgg tgcatctgct ttcgcctatg agtttgtggg cgaaatatgg agcaaatgct
     6901 ccggtgaaag cggtaatgtg gaatcacttg gcaggttcgg ctttaacggt tcgccctgaa
     6961 atcaacagta ttgccgaact ctccggcaaa acggtagcaa ttccgttttg gtattcaatt
     7021 cataatatcg ttttgcaacg attattacga gaaaatggct tgaaaatcac cgaaaaagag
     7081 ccgaaaagcg gtgaagtgcg attaagtgta atggctccgt cagatatggt ggcagcgttg
     7141 gcaagtgagg caatttccgg ctttattgtg gcagagccgt tcaatgcgtt agcggaagcc
     7201 aaaggtgtgg ggaaggtgct gcgttttagt ggcgatgtgt ggaaagatca cgcctgctgt
     7261 ttaaccctaa tgcacgaata cgatattcaa caccgcccgg aatgggtgca aaaagtggtg
     7321 aatgtcttaa ccgaagccca agtgtccacg cttgccaacc gtgcagaaac cgctcaatta
     7381 ttggctcggg gcagcggcta cacgccgcac gatcaaaaag tgctggaaaa ggtactggct
     7441 ccaacccaag ctcagtggga taaatatatc gaaacagggg caattcaaaa ccctcagtgg
     7501 caccaacagc gaatcggttt ccaaccgtat ccgttcgatt cctatatgga agagctggtc
     7561 aaaatgctca aacagaccca cattgccggc aataatgcgt ttttacaaca gcttgacccg
     7621 gtagaggcag cgaaagcgat taacgagccg acctttgtgc gaaaagcgat tgagcaaggc
     7681 ggttgggttg aaacctttaa tttacaaacc ggttggacaa gaaccgagca aattagtgta
     7741 taagcgatag gaacgaacga tgaaacagaa atatatttac ggcttagccg gtttattact
     7801 gctactttta ggttggcagc ttggcacagc cttgttaagc caacggttgc cgatggcgtc
     7861 aatgttagct ccggcaaaaa cctgggaaag ttttacttgg ctgatttcct ccggcgaaat
     7921 ttttccgcac ttatgggcga gtttgcaacg cattgctgtc gggctactcg ctgcgttagt
     7981 gattggtgta ccgctgggct tattgctcgg cttctccaag caccttgaaa tgctttgcac
     8041 gccgtcattc caactactac gtatgatttc gccactttcg tggatgccga ttatcgtgat
     8101 gttacttggt gtaggcgatt tgccgattta ttttttactg gcgtttgcca ccgtttggtc
     8161 gttgattatt tccaccacct caggggtaaa aagcattgat ccacaatggt tacaactggg
     8221 tagagtaatg tcggctaccc gcaaagaact gctgtgcaaa attgttgtac cggctattac
     8281 cggacacgtt ctcaccggtt tacgtgtggc ggttgggatt gcgtgggtgg tgctggtacc
     8341 gtgcgaaatg ctaggcgtga atcaaggact cggctatttt attttagaca cccgagaccg
     8401 attagcctat tccgaactga tggcggtaat tataattatc ggcttaatcg gctgggcgtt
     8461 ggatagctta atccgcttgg ctttagcgaa aggcggcaga taaattgaca agcggtcgaa
     8521 aaattgcatt tttttgcaaa aaatcgaccg cttgtttatt cccaaaaagc atattacctt
     8581 aatttatttt atttctaggg cataaaaaag cgatttaaga tagcgacaaa gtaaactaat
     8641 tatacgaggt aaacacaatg tcggttcttt catcttcttt tattcaatgg cttcagcaga
     8701 acgccgaaaa tttagatcaa tctaacgaac gagcggattt actcattcca caaattgccg
     8761 cactagggct atataaaatc ggcgtgccgg aaacgcttgg cggcagcggt ggcactttgg
     8821 ttgatgcgat caaagccgtt accgaactgg gtaatcactc gctggctgcg gcatttgttt
     8881 cgtggggaca tcgtaccttt atcgagcata ttatcgcctc aaaaaatcct attccccgcc
     8941 aaagctattt ggcggatctg ctaaatggca atttaaccgc aggaacaggt ttatccaatg
     9001 cgatgaaatt tctctccggc attgaagagt tgaatgtgaa tattcgtgaa gaaaacggta
     9061 aacgctattt aaatggccgc ctgccttggg taaccaattt acgttcatac cgctttgttg
     9121 ctagtttcgt tgccggtttt caagacgaac gcaaaccgct agtgattgca attccctcca
     9181 ccgccaaagg cttaacccgc accaaagaat tggaatttat cgcattgcaa ggcacgaata
     9241 ctacctctct cgtatttgag aatgtggaat tacaagatga atggattttg gctgatgacg
     9301 cgcctgagtt tttagcagcc actcgccctg cgttcttagg tttacaattt ggtttgccct
     9361 ttggcttggt tgaacgtagt ttggcggaaa tcgaaaaatc gctccctttc cgtgcaattc
     9421 tcgcctctga gtggcaagct caagttgatg ccctcaacga tattaaaact cgtctgttta
     9481 atggcttaaa tgaagaaggt tattttgtgc gtaatccgaa agagcttttc caagcgagaa
     9541 ttgacattgt caatgtcgtc gcccaaacta ttctgctaga actgcaagcg agtggcggac
     9601 gtggctatct gaaaaatgat acttcaggct tcagccgccg ttggaaagaa gcggcatttc
     9661 tgcccgttgt tacgccaagt gcggtacagc tgagaatggt gctggcgaat agttaagcgg
     9721 ataagagaca aattgttgcc tcttttattt tgcaaaaaat agtctaaata taaccgcttg
     9781 caataaaaat aaaaaaggat gcatttttta tgcttcctca attttacggc tatctattat
     9841 gcaaaaggct gcaattcagg gttaattgga atttcggcaa tgttgttaac gtagttacat
     9901 aatgttgcca agctcacgcc taaaatcaca tcaatcgcat tttgttggtt gtaacctgcg
     9961 gcaaaaaatt cgtttaattg ggcttcggtt aatttggctt tttgtaacat tactgcaagg
    10021 gtgaagcgag ccagagtgtc taatttgtta tcgtctatgc gagccgtggc acgtaaagca
    10081 tttaccactt cttcaggcat ttttaatgct tttaaggcga ttttagtatg acctgccacg
    10141 cagaaaccac agccgttgac cactgctgcg gtgatttgta ccacttcacg ttcttcagcg
    10201 gttaagctgt tacgactatt aataccaccc acagtgcggt aagtttccag tgctgttggt
    10261 gcgttggcta atacgccgat cagatttggg ataaaaccgt ttgcttgttg cactgctttt
    10321 agggcttctt gtgccgtttc aggagcggat tcaatagtgt gaatttgaaa ttgagacata
    10381 aatgttcctt atcaggttgt ttattggtgt ggctcatcat aaagaaaagg ctaatttaga
    10441 acaaatcaaa atcactattt aatataacta aaagatctta aagggagtat agttgcctat
    10501 actccctttg tttatgcaca ggttgcgttt ttactcttct gcgtcttctt ttgcccattc
    10561 taatgagcgg cgaacggctt ttttccaacc tttatagcgt ttcgcccgtt tttcttggtc
    10621 gccgtccggt gtgaaggtgc gttcaatttc cgctttgtct ttcagttctt ccaaatcttt
    10681 ccagaagccg acagcaatac ccgcaagata agctgcccct aaagcagtga cttctttcac
    10741 tttcggacgc tcaactttgg tgtctaaaat atcggcttgg aactgcatta agaagttgtt
    10801 tgcggttgca ccaccgtcca cacgtaacgc ttgtagtttc gctttggagt cagactgcat
    10861 tgcttctaac acatctcggg tttggtaagc gatagactct agggtcgcac gcacaatgtg
    10921 gttacggttt gccccacggg ttaaaccgaa aattgagcct cgtgcatacg gatcccaata
    10981 tggagcacct aagccggtaa aggcaggtac aacataaaca ccgttagagt catcttcttt
    11041 agtggcaaaa tattcgctgt caaagctatc gtgaacaatt ttcagctcat cacgcagcca
    11101 ttgaatagac gcaccggcaa taaatactga accttccaac gcatattccg gctcgccttt
    11161 tgcgttacac gcaatggtcg ttaataaacc gttttcagat tgaatcgcag tgttaccggt
    11221 gtgcatcaac ataaaacagc ctgtaccata ggtattcttc gcttgtcctt ctttcacgca
    11281 taaatgaccg tagagagcgg cttgttggtc acctgcaata cccgcgaccg gaatacgcac
    11341 accgccttga ccgccgatat tagtttgccc gtaaacttct gatgaatttt tcacttcagg
    11401 taacattgag cgtggaatat tgagcaattc cagcatttta tcgtcccatt gtttggtgtg
    11461 aatgttaaac aacatagtac gagaagcgtt ggtgtaatcg gtaacgtgta cccgcccttg
    11521 ggttaatttc cacaccagcc aagtatcgac cgtaccgaac agcaattcgc cacgttctgc
    11581 tttttctctt gccccttcta cgttatctaa aatccacttc actttagtgc cggagaagta
    11641 cggatcgact accaagcctg tcgttttacg aatatattcc tcgtgtccgt ctgcttttaa
    11701 tttatcggta atatccgatg tgcgacgaca ttgccacaca atcgcattat aaacaggttt
    11761 accggtctct ttttcccaca caatcgtggt ttcacgttgg tttgtgatcc cgattgcggc
    11821 aatgctatcc ggcttaatac cggcttttgc caccacttca tttaaggtgg aactttggct
    11881 tgaccaaatt tccatcgggt tatgttctac ccagcctgct tgcggataaa tctgggtaaa
    11941 ttcacgctga gccacttcaa ccacattggc atttttatct aataaaaccg cacgagagct
    12001 ggttgtacct tggtcgagag cgatgatata ttgtttttcc atagattact cctgagtatt
    12061 tgctggaaga ttgttatgaa tgaggttttt ataaccccaa gcaccgaata atgcaccgca
    12121 gattggggcg acaatcggaa tgatgaaata cggaatttct tttccgcctg ttaaagcaat
    12181 ttcaccccaa ccggcaagat aagcaaagag tttcggacca aaatcacgag ccgggttcat
    12241 cgcaaagccg gttaacgggc cggttgctcc accaattacc gcaatcaata aaccgatgag
    12301 taatgcggct aaggctttat tcggcaagcc gtttttatca tcgccaatcg ctaaaatagt
    12361 ggacatcaac accacagtaa tcacaaattc aacgatgaag gcggtaacca atgtaatatt
    12421 agggtttgga taggtggaga acacaccggc aaagccaaca gtttcgcccc gagtaatgcc
    12481 ttgagcggct tcggcggcag taaagagatc gctatacata aaataaatca gtgcgacaga
    12541 gcaaaacgct ccggcaactt gggcgatgac atagggcaaa attttatgct tttcaaagcc
    12601 gtagaatgcg gcaagtgcta tagtgaccgc agggttaagg tgagcaccgg aaataccggc
    12661 agaaatgtag attgccatgg aaacaccaag cccccaaacg atggaaattt cccataaacc
    12721 gaaattcgca ccggctacct gagcagcagc aacacaacca gcaccaaaga aaagaataaa
    12781 acctgtaccg ataaattcgc caatacaggc attacgtaag gatttgctca taaagtttct
    12841 ccttaagatg aaaatgattg gaaagtgatg aaaaaatgtt tgaatatgaa catatttcgg
    12901 agtgatattg ccaaagcggc attttttaag caaataggaa gtttcaaatt tgtgaaatcc
    12961 atcacattat ttttctaaat cactcatttt taggtatgat gtgagaaaac gttagatgag
    13021 gaaaaatgat gtcttcaata cttatgattt caatcggggc ggtacttgga gctatgtgcc
    13081 gctggcaatt agccgtttgg ctaaacccga tactcagcca attcgccttt ggtactttag
    13141 cagcaaattg gcttggttgt ttgttaattg gtattgcaat ggggtttaat ttaggcgaga
    13201 gccagcggtt gctgttcatc agcggttttt taggcagttt taccaccttt tccagtttct
    13261 ctttagaact aacggaaaaa ctattagcag aaaaatgggg gcagtttgtg acagtgctga
    13321 gtttacattt aatcggtggg cttttgctta ctttgatagg tgtaatgctt gcaagagccg
    13381 ttacaagcgg tcgtttttgc tgaaaatttt gcaaataaaa aacctcccaa acgggaggtt
    13441 ttgcgagcga ttaaagtagg atattggaaa gtaataaacc aaatccgata ctgaaaatca
    13501 tactaagtaa gcccggtaac ataaagctat ggttgaaaat atatttaccg atacgggttg
    13561 tgccggtggt gtcgaagtca atcgaggcaa taatcgggcc gtagttcgga atgaagaagt
    13621 agccgtttac cgccacaaat accccgatta gcaccggtgc aggaatgccg atagcaatcg
    13681 ctacagggaa taaggttgca accgttgccc cttggctgtt taccaatacg gaaagtacga
    13741 ataatgcgaa agcaaatgcc caaggtgcgg tttcaactaa gcctgaaacc attgatttca
    13801 cttcgtccat atgaccttgc attaaggtat cgcctaacca agcaataccg aaaatcgcaa
    13861 tcactgcacg cataccggcg tggaagacag aacctttggt gatttcatta ccgtccggtt
    13921 tgcagaagaa gataatcaac gcaccaatac ttaacatcac aatttcaatc gtgtgagcca
    13981 tccccatcgg cttaccatca aacacagggc gaagagctgg aattgcgccc attaatacca
    14041 ctaataatgc accgaatagg aataagccta ctgataattt cgcagtcggt tttaacggta
    14101 attcggttgg attggtagta atgtgatttt ctttcacata ttcagggtct tgcaataagc
    14161 gttgataatt tgggtcgtct tttaattctt tgcccatttt atttacaaac acacaagcta
    14221 aacctaagcc caataaggtc gcaggaatag tcacacttaa cacgttaccc aatgtaacac
    14281 cttgaggttc aaggaaggca accacagcaa ctactgccgc agcaatcggg ctggcgacaa
    14341 tcgcaaattg tgaggcgatt actgccattg aaagtggacg ctcagggcgg ataccgttgt
    14401 ggcggcttac ctctgcaatt acaggtaata cagaataagc aacgtgacct gtaccggcaa
    14461 gcacggtgaa tgtccaagtt acaagcggtg cgataaaggt aatgtatttc gggtttttac
    14521 gcaaaatgcg agtggcaatt ttgatcatat agtccaaacc accggctgct tgcatggctg
    14581 ctgcagccga cacaaccgcc ataatcataa acattacatc aatcggtaaa ccggcaggtt
    14641 ttagcccaaa cccaaatgaa agaatggcta agcccaagcc accgaacacc cctaatccaa
    14701 taccgcctac acgggcgcca accaaaatgc atagtaacac tatggcaaat tggataataa
    14761 acatcgcaga cataattttt tgctcctaaa taagtaaatg aaactctgtg tttgaaaaaa
    14821 tctgcatttt tcatcaaatt aatcggcacg tactatatca ataaataaaa ctaatctcta
    14881 taatctaaat caataaaatt tctaattaat taaggaataa tatgcaattt tcaaaaatgc
    14941 acggcttagg caatgatttt atggtggttg atggtgtaac ccaaaacgtt taccttaccg
    15001 aagagatgat ccgtacgctt tctgatcgcc atcgtggtat cggtttcgac caacttttat
    15061 tggttgaacc gccttacgac cctgaattag attttcacta tcgcattttt aatgcagacg
    15121 gcagtgaagt gtcgcaatgt ggcaatgggg cgagatgttt tgcccgtttt gttacgctca
    15181 aaggcttaac caacaaaaaa gacattcacg ttagcaccgc aaaaggcaaa atggtgctaa
    15241 gtttgcaaga agacggcaaa gtgcgtgtga atatgggcga accgatttgg gaaccttcgc
    15301 aagtgccttt tacagcaaat aaatttgaga aaaactatat tttacgcacc gagctacaaa
    15361 ccgtgttatg tggcgtggtt tcaatgggga atccgcactg cgtactgcaa gttgatgatg
    15421 tgaaaaccgc cccggtaaat gaactcgggc cgttactaga aagccacgag cgttttcctg
    15481 aacgggcgaa tatcagcttt atgcaagtag tgaatcgcaa tcacgttaaa ttacgggtgt
    15541 atgagcgtgg agcaggcgaa acccaagcct gtggtagcgg tgcgtgcggc acagtggcgg
    15601 tcggcattat gcaaggcttg ttagataaca aagtgcaggt ggatttaccc ggtggaacgc
    15661 tgattattga atggcaagga gtagggcatc ctctgtatat gaccggcgat gccacccaca
    15721 tttatgatgg atttattaaa ctgtaagttt tcccataggg aggcaagcgg tttattttta
    15781 ccgttttttt gcaaaatagg gttggtttat acaaaagtac cagcgtggac gctggcacta
    15841 tcagactgat gtataacttt tgtttaaatt caacttatag atggtgcaag cgtcctcgct
    15901 tgtaccaaaa caggtttcac tcgagtaact tcttcaccaa ctccaacacc acaggtaatt
    15961 cggtaacacc ttctctatcc aaaaaatgcc cgccttgatt taaccgaata taatccgcgt
    16021 gcaaatgttg ggctaacgca tcactgtatt ggtgattcac gaccttatca tctaacgcac
    16081 ttaccacata gcttggaaag ctcgatacgc tcggcaaaat cgcataatag ttggtaaagg
    16141 cagacagttc gggtaagttt tctaccggtt gatagaaccc cgacacaaac accgcccctt
    16201 taatcttggc gtaatttttg gctaaaaaat tcaaaatggc aatgcaacct aaactatgcc
    16261 cgacaaaaac cgtttgatcg gtcagctcca cattttgtgc aagatgattt aaccaaccat
    16321 ctaatgacgg ctttgccgaa tccggcatat tcaaccgctc acaactgaca ccacacttcg
    16381 ctaattcttg ctccagccaa ggaaaccaat gcttatcggc attcgccgta taaccgtgta
    16441 cgatataaac ctgtgccatt tcacacctcc tgattcacca aagattgtac catagcttaa
    16501 tcaggaaaaa acaagcggtg atttttttaa atttttttgc aactcaggta aaatagcacg
    16561 catttacgca aagaggtcat caatgaacgt ttggatttta cttgcgattt ccatctgcct
    16621 cgaaatcgct gccaccaatt tactcaaatt aagcaacggc ttcaccaaag ctataccgac
    16681 tattggctcg ctcgccttat acggtttatc cttttacttt ctctctatta ttttccgcac
    16741 cctaccggtc ggaatcgtgt atgctatttg gtccggcgta gggattgtcc ttaccgccct
    16801 tgttgcttat tttgctttcg gacagaaaat tgacttggca ggcttaatcg gcatcagcct
    16861 cattattgcc ggtgtattgg tgattaattt gttttctaaa gtgtgataca agcggttgat
    16921 tttacaaaaa aatctgcaaa atcaaccgct tgtaatagtt ggcaagaatt ataacccctt
    16981 taaccgccgg taaagctcag taaatttttg gcgtttttgt tgatattctg catagcgggc
    17041 aggggttggt tggtaaacct gttccagggg taacggctga cagaacgtag caatatcctg
    17101 atttgggttt agcgcaattt gagcgagttt tgccgcccct aatgccggac cgacatcacc
    17161 actggtgcgg tattcaaagg ttttaccgct aatatcggca agtagttgtc gccagtacac
    17221 actttttgct ccaccgccaa ttaatgcaat attgtcagca actacaccag tttcgtggag
    17281 tacctcaatc ccttcagcca aagcgaaact taccccttca atcacggctt ttgccatcgt
    17341 tatttggttg tcattatggc ttaatcccca aaatacgcct ttcgcataag ggtcattatg
    17401 cggtgtacgt tcacccgata aataaggtaa gaaaatagca tcagatgccg tttggcttgc
    17461 ttcaacctgt tggaataaag tcgatacatc ttttagtccc aatgatttta ctgcccaatc
    17521 taccgcagaa gcggcactta atattactga catcagatgc caacgatcag gtaaagcgtg
    17581 gcaaaaacta tgcactgctt tttgcggatt agcctgaaat ttatcgctca ctacaaaata
    17641 aacccctgaa gtccccaatg agagcatagc ttgcccggtt tggtataagc caatgccgat
    17701 tgcaccggca gcattatcgc caccgcctgc cacaatcggc acagatttca ttttccattg
    17761 ctcggcaagg gttggacgta aataacctgt gatttgattg ccttcaaaca gtttcggcat
    17821 attttcaatg tctaatccgc aagcatttag tagcgatttg ttccaattcc gtttacccac
    17881 atcaagccac attgtgccgg aggcatcaga catatcagag gcatattcgc cgctcatcaa
    17941 caagcggaga taatctttcg gtagcaagac tttgctgact ttttcggcaa tatcgggctg
    18001 gtgtttatca acccaacgca atttcggagc ggtaaagccc ggcatcatca ggttgcctgt
    18061 aatttcacgg ctatttggca caagctgttc taactcttcg cattccataa aactacgtcc
    18121 gtcattccat aaaatcgcag gggaaagtac attatctgcg ttatctagta aagtcgctcc
    18181 gtgcatttgc ccggttaaac caatcgcttt tactgcttgt aaatcttgag tagcggaaag
    18241 ttctaacatc gcttcattgg tggcattcca ccaatcttgc ggattttgtt cagaccacaa
    18301 tgggtgtgga cgaaaaatag gaagtgattt ttgtgtgatg gcaataatgt gttgattttc
    18361 atcgagtaac acgactttca cacccgatgt gccgagatcg attccaatgt acataaatat
    18421 tcctttgtta ttgaatacaa gcggtcattt tattcggaac atttgcaatt ttaagttttg
    18481 gagatctatc acccctcttt agcaaagagg gggatcagat cgagcttaac tttgcaaaaa
    18541 attgataaaa aacaaccgct tgtagattaa cggtaaatat agctatttac caaattttcc
    18601 aaatattctt gctgaccgga aacaggtttt ggatctaacc cttgggtttc cactattttt
    18661 gccaaatctt caaggcttgc tttaccgttt agaatttgta agcccaaatc ttggttccag
    18721 ccagcgtagc ggttatctac tactttctgt aatgcttgat cttccagcat ttttgccgca
    18781 cgttttagcg aaagggctaa cacatcaatt gccccgatat gtgcgtggaa taaatcatac
    18841 ggatcggtac tttggcggcg gattttcgca tcaaagttga aaccgcccgt tgtgaaacca
    18901 cctgctttta agatttcata catcacaagg gtgttttctt ccacgctgtt tgggaattgg
    18961 tcggtatccc aacctaattg cggatcacct cggttggcat caatcgagcc aaaaatatct
    19021 aatgcggtgg cagtcgcaat ttcgtgttgg aaagtatgcc ctgcaagagt ggcgtggttt
    19081 gcttcaatgt tgactttgat ctctttttct agaccaaact gttttaagaa accgtaaacg
    19141 gtggcaacat cgtagtcata ttgatgtttg gttggttctt gtggttttgg ctcaattaat
    19201 aaagtgccgt taaaaccgat tttgtgttta tgttccacca ctaactgcat aaagcgaccg
    19261 atttgttcac gctcacgttt taagtcagtg ttgagtaggg tttcataacc ttcacgtccg
    19321 ccccataata cataattttc gccgcctaag cgtttggttg cgttcattgc ggtaaagact
    19381 tgtgctgccg cccaagcaaa gacttccgga ttcggattgg ttgaagcacc cgacatatag
    19441 cgaggattgg taaagcagtt cgccgtaccc cataataatt tcacgcctgt ttcagcttgt
    19501 ttttgctcta aaatatccac aatagtgttg aaattgtgct gatattcttt gaaattgttg
    19561 ccttccggtg caacatccac atcgtggaag cagtagtaag gaatgcctaa tttttggaaa
    19621 aattcaaagg caatttccgc tttttgcttc gcaccttgca gtaaatcccc cgttttttgc
    19681 cagcttctgt caagcgaacc cacgccaaac atatcgttac ccgtccagca gaaggtgtgc
    19741 caatagcaaa ccgctaaccg cagatgttct gccatcgttt tgcctaaaac cacttcatcc
    19801 gggttgtaat gtttgaatgc aaacgggttg gtggaattag ccccttcata ttgtactttt
    19861 gagattttgt cgaaatagtt agacataata atgctcctat gaattggaaa aataatcttt
    19921 cggtaacatc atcggaaaat tatgcaaaac cttcaattac gatatttcgt ttttggattt
    19981 cgattattgg ttaatgtggc gtagatcaca aaagtgaaaa atcataatga tattaataaa
    20041 tatgataatt gaacttcatt tgaaataagc ccagactgct tacgggcgaa aaagatgctc
    20101 acattttcat aaccaatgag gtgaactatg aaacttaagt ctaaattatt agccgttgct
    20161 acggcgactt taatggtact cagcaatgct tctgttgcta aagatctaaa aatcggtatg
    20221 tcaattgatg atttacgctt agagcgttgg caaaaagacc gagatatttt tgtgaataaa
    20281 gcggaaagct taggagcaaa agtcttcgtt caatcggcaa atggcgatgc aaccgcacaa
    20341 atctcacaaa ttgagaatat gttgaataaa gatattgatg tattggtgat tattccgttt
    20401 aacggcgaag tgttatccaa tgtgattgcc gaggcgaaga aagaaggtgt taaggtactt
    20461 gcttacgacc gcttaatcaa taatgccgat gtggattttt atgtttcatt tgataatgaa
    20521 aaagtaggtg aattgcaggc taaaagcatt attgggcaaa agccggaggg gaattatttc
    20581 ctaatgggtg gttcacctgt agataataac gcgaagctgt tccgtaaagg tcaaatgaag
    20641 gtgctacaac cgttaattga cagtggaaaa attaaagtgg taggcgatca atgggtagat
    20701 tcttggcttg ctgaaaaagc attacagatt atggaaaatg cactaactgc taataaaaat
    20761 aatattgatg cagttgtagc ttcaaacgat gcaaccgcag gcggtgcaat tcaagcgtta
    20821 agtgcccaag ggttatccag caaggttgca atttccggac aagatgcaga tttagccgca
    20881 attaaacgta ttattgccgg tacgcaaacg atgacagtgt ataaaccaat taccaattta
    20941 gccgacaaag ctgccgaaat tgctgttgca ttaggtaaag atgagaaagt agaaatgaat
    21001 gctcaattaa ataacggtat gaaagaagta ccggcatatt tattagagcc ggttgttgta
    21061 actaaagaaa atattgatga cacagtaatt aaagacggct tccataccaa agatgcagta
    21121 tataaataaa tcactttaat ttttctagat ggcgaggctt cctcgccatt tttgaaaata
    21181 aggggctaac taagtatggc gaaattatta gaaatgaaga acatcactaa aaaattcggt
    21241 gaggttgtgg cattaaataa tatttcaatt tcccttgaaa ccggcgaaat actttcttta
    21301 tgtggtgaaa atggttcagg caaatcaact ctgatgaaag tattatgtgg aatttatcct
    21361 gccggtgatt atgaagggga aatctatttt tccggcgaaa aattgattgc cagaaatatc
    21421 aaagatactg aagaaaaagg catttcgatt attcaccaag aattgacctt agtaaaaaat
    21481 atgtcgattt tggagaatat gtttttaggg aatgaaatta ccaaatcagg gattacagat
    21541 gacaatgaaa tgtatctgcg ttgtaaaacc ttacttgaac aagtgcagtt ggatattgac
    21601 ccaaacacca aagttggaga gctaggttta gggcaacaac agttggttga aattgccaaa
    21661 gcattgaata aacaggtacg gttgttgatt ttagacgagc ctaccgcctc gttaaccgaa
    21721 aaagaaaccg agattctgct gagtttaatt aaagatttac aagctcacaa tattgcttgt
    21781 gtttatattt cacacaaatt gaatgaagtg aaagccattt ccgacaaaat ctgtgtcatt
    21841 cgagacggcg aacatatcgg tactcgcagt gcagagggaa tgagtgaaga cgatattatt
    21901 acgatgatgg ttggacgtga gattacctcg ctctacccac acgagccaca caatattggt
    21961 gaagaagtcc ttcgagttga aaatttaacc gcttggcacc caaccaatac acatatcaaa
    22021 cgggttgata atgctaattt tgtactacgc caaggtgaaa ttttaggtat tgctggtttg
    22081 gtcggttcag ggcgaaccga gatggcacaa tgtatttttg gctcttacca aggcaaatat
    22141 caaggtgata tttttctgca taagcaaccg attaaaatca aaaattgtgc acaagcgatt
    22201 gaaaaccata ttgtgatggt gccggaagat cgcaaaaaac acggcatcat tccaattatg
    22261 ggggtgggta aaaacataac tctctcttca ttaccgcaat tttgttttgg caaaaaagta
    22321 ataaatgaac cgctggaaga aaccattatc aaccaatcta ttgcaaaact gaaagtgaaa
    22381 acttcctcgc ctgaattagc aattggacgg ttaagtggcg gtaatcagca aaaagcaatt
    22441 ttggcaaaat gcctattgct taaccctaaa atcctgattt tagatgagcc gacccgtggt
    22501 atcgacgtgg gagcaaaata tgaaatttat aagctcatca accaattagc taaagaaggt
    22561 atggcaatta tcgtcatttc ctccgaatta cctgaagttt taggcattag tgatcgtgta
    22621 ttggttatgc accaaggcaa aattaaagca gatttgatta accaagacct tacccaagaa
    22681 aaagtaatgg aagcggcact caaggagtaa cctatgaata agttaaaatc gattaattta
    22741 caagtttata ttatgctgat tgcaattgtt gtgattatga gctttttctc tattgcaact
    22801 gacggtgctt atttaagtgc cagaaatatc tccaacctgt tacgccaaac ctccattacc
    22861 ggtattcttg ccatcggtat ggtatttgtg attatttccg cagaaattga tttatcggtt
    22921 ggctcgctaa tgggcttact cggtggattt gccgccatca gtaatgtatg gtggggctgg
    22981 ccgctctcgt taactattat agttacttta attttaggcg tcatgctggg tgcttggagc
    23041 ggttggtggg ttgcttatca aaaagtgccg tcctttattg taactcttgc cggtatgctg
    23101 gcgtttcgtg gtgtattggt cggcttaacc aacgggacaa ccgtttcacc gattagcaat
    23161 gaaatgacaa ccattggtca aggctatttg cctgatatgg tgggtatgat tttaggtact
    23221 atcgggatat tttgctttat tgtttggggt agctaccaac gcaaagccag acaaaaactc
    23281 aatcttactg tgccttctat gtcgaaagac acgctgaaat acggactagt tgcaattatc
    23341 gtattaggtg cgatctacct actcaatgat taccgtggtg tgccattccc ggtattggtg
    23401 ttggtgattc tagccattgc cggcacattc ttggccagaa aaaccgcctt cgggcgtcac
    23461 atttatgcga ttggtggcaa cattgatgcc gctcgcctct cggggatttc agttgaaaaa
    23521 gtcaaacttg cgatttttgc cttaaacggc ttattagttg ccattgcagg cttgatttta
    23581 agctcacgtc ttggagccgg cgcaccttcc gccgggcaaa atgccgaatt agatgcgatt
    23641 gccgcctgcg tgattggtgg agccagttta gccggcggta tcggtagcat ttatggcgta
    23701 gttattggag catttattat tgctttgctg gataacggta tgagtatgct tgatgtgcca
    23761 accttctggc aatatatcgt taaaggtgga attctactgc ttgcagtttg ggtagatacc
    23821 atcagcaaga aaaaagccta attggataac aagcggtcaa tctcgactat ccaacgggaa
    23881 atccggcata atcgggctaa aaatgggaac tattgtgcgt tttgccgtct ttttagcatt
    23941 tgctacggca accagctcca ccaagtgcgg tggcatttcg ccttgtttgg caaggttgca
    24001 aaggtaggtt atcgctttaa tccggctcat tgagccttcc agttccaaca catacgccac
    24061 cagtgccatt cgtgcatcag caatttctcg ctgtttggtg gtcaggtttc ccacatccac
    24121 ctccgcacgc actgccggta actttttcgg cttggcttcc accaccgccg acataaagcg
    24181 ggagcggatt tcggtttgga tttcttgagg gaggtttgag agtgcaaatt ctaaaatttc
    24241 tcccccaact ccaacctttt tatgagtagt ccatccttgt ttctttgcct gatacaaaac
    24301 tccttgcttt gttttaggta aacaggctaa acttaactca gccaattctt ttgccgaata
    24361 gtgtgttttt agactgtttt cactcataaa aacctcttta agctatctgt cggcaaattt
    24421 tttatggttt cgttgagcat aacgttcagg ccaaatttct tcgggtgata taccgattgc
    24481 ttcggcaata atacgctcac cctttaacca cggacgatct acagcgtttt tcaaggtgct
    24541 ggattgactg tagccatgct tcaatgagag ttgtctaagc gaccaacccg ctttatgtaa
    24601 agcagcgatg atgtcggccc tatgccagtc tttcgctgtt tttttaatat cttccaatgt
    24661 actcattaga taactccttt cgcttaacga tttagataat atacctattt ttactaaaaa
    24721 taaagtgtaa aaaggttgct ttatagttga tttatgagca aataaactta gtttgctgta
    24781 agttattgat ttatttatag gtttttagag tgtaaaaggc taaaaatctc aacttatttt
    24841 tttacagtta tgaaaataga gtgtaaaaag gttattggtt tcggtaaccg aatagaacag
    24901 agcagaaaaa atgcaaaatt aagtagatct gaggtttgtg atgccctagg aggtattgca
    24961 atatctacat tacaagcgtg ggaggctgat acaagagaac ctcctatatc aaaacttatg
    25021 gctttgtcac aaatacttaa cgttgagcct tgctacctat taacaggtga gcgacactcg
    25081 caaagcctta atgtcacaag agagccacag cctgtgtatg ccacaaaacc gcagacagaa
    25141 gaaggggaag tagttgttat caaagctaag ttacaagagg caatagaaac gttggaagag
    25201 gtgctggaaa ttacccaacg ggaaatgaag cctgccggaa aagcccaaat ggtgtgggct
    25261 ttctacgagc ttctcacgca agaaaaaagc gaaaaagaaa aaatggtggg attactcaaa
    25321 ctggtggcat aacaggggac aatatggaca aacaagccat tctcaaatta ttcagagaca
    25381 atagcactgc cggcaacagc cagccaagcc aagagcctat acaggtgttt aataacagca
    25441 acgtcatcat tcaaaacggc gataacgcca cggtgattgc cggctgcacg gtggataata
    25501 acaccaacct aacctgtgcc gataaacaac gtgtacacga cctcattcac agcttagagc
    25561 aacgcaaccg agccagccga gatgagggcg cttaaaattg ttttaaaatc cgtttaaacc
    25621 tgctcagacc tatgcaaaaa ataacgtaaa aatagcccat ttttttctat ttttgctcat
    25681 tttcgttttt tgcataactc accccaaaca aaaacggcga agccttgtat ttcctagcct
    25741 cgccgttatt tttccgccca aatttttttc tttctctcta tgcaaaaaag atcagtaccc
    25801 cacactatgt tgccagacat actcaacaat tttaccgtca acgtcagatt accaaaaatg
    25861 accgcttgta atttagagga gtgtttagag cggaaataac atcggaatca caaacaccga
    25921 caccagcatc accagtacag taaacggcac accaacttta acgaaatcaa taaatttata
    25981 gcctgccggt gctaatacca tcgtatttac cggtgaagaa accggcgtca taaaagcggc
    26041 ggaggctgaa acagcgaccg tcatcacaaa aggtgccggt gagtagccaa gttggttagc
    26101 aatcgcaatg gctatcggca taaccaaaat tgcagtcgct gtgttggaaa taaaagcact
    26161 gataatcgct gttactacaa ataatactat tagaacaaaa tgtacatcta agccttgcat
    26221 tgcatcaagt agaagcctaa cggttaaatc cacaccaccg gttttttgta aggcaataga
    26281 aaacggcatc atccccacaa tcaacacaat gcttggcata tgaatcgaat cataagcact
    26341 tttcatatcc acacagcgga acttgcctaa cattaagcag gcaatcaacg ctgccatcac
    26401 gtttggcaca atgccggtaa tcatcagcaa caccatagtc agcactgaga acagagcgtg
    26461 aggggcttgg cttaacgccg gaggggcgtt tttactttct gccggatctc taagcaagaa
    26521 gaaatctcga tgatcgtcgt ccatacggtg aatttgttgc catacgccca tcaccaataa
    26581 aatatcgccg aatttgagtg gcgtatcgag cagttcctct tgtatgattt catcaccacg
    26641 ttttaagccg acaatgctta aatggtatct tgagcggaat ttgagttccc gaatagtttt
    26701 accgatacat tctgcttcag gcataaccga aatttccgcc atacccaccg agcgatattg
    26761 tgaggaaaag tattcgcctt ttagctcaat cgggcgaagt ttgagctgtt cacagcgttg
    26821 ttggtaagtt tcatcatcga gggcagtatc aagcagtaaa atatctttcg gctggaaaat
    26881 ttcgtttacc gaggcactga cggtaatttt tctaaaccgc ttagtgcgtt gaattgcaat
    26941 gatgtttagc ccttgctcaa tccgtaaatt cagttgcccg atagatttgc cgacacagtc
    27001 tgaatctgct tccaccagca ccattttggc tcgctcttta agttgatact cttcaattaa
    27061 ctgcgacatt gaaaattttt gagcctgact gtgagtattg ctttgctcac cattatccaa
    27121 ccaacggcgg gcaatcagca tatagccgat gcccatcact aaaataatta atccaatcgg
    27181 agtaaaactg aagaaattga agccttcaaa acctgatttt actaattcgg aatgagcaac
    27241 aaggttgggt ggtgtggcaa ttaaggtcat cataccgctg ataagtccgg caacacttaa
    27301 cggcatcatt aaccggcgag gagaaattcc cattcccgag caaatggcaa gcaccaccgg
    27361 aatgaaaatc gccacaatgc cggtggaact cataaaagat ccaagcccgg caatacagag
    27421 cattagcagc accagcactt tggtttcgct cgctcccgac acccgcatca accaatcgct
    27481 gacttggttc gccacgccgg ttcgcaccaa accttctccg acaataaaga gcaacgcaat
    27541 cagcaccacg tttgggtcac ttaagcctgc caacacttct ttgactgata aaatattagt
    27601 caggctaaaa aacagcatta caattaaggc aaccacgtcc atacgcaact tgttttggat
    27661 aaattgccaa atcgcaaata ccaataacgc cattacccaa aacagttctt gcttaagaaa
    27721 atccggcaca aaagagagca acgattgcat aaccatatcc tccaaaatag ttagggatag
    27781 attacaccgc tttaagcaaa gaaaaaagcc gataagatca aaaagaaagg aaaaagcaat
    27841 cacgtacggc gtgaatgctc agtacaagcg gttagttttg acgaaatttt tacaatgggc
    27901 tttccacatt cagttgttcg tcaatttcag ttaccgccaa aatcaggctt gaacgtacca
    27961 ttttttccaa ccgcatttgc tgcatttgaa agagcatcga atctttattc gctgcactcg
    28021 ctttcatatt cagcaattca ttgataggca aaaaatcaat atggtgcaaa tctttggcaa
    28081 atttcactac cgccgggtga gaaaatgcga actcgtcttc ttcctcattc aaatgctgtt
    28141 tgacttcaag gaaaagcgag atatcttcaa acactttagg tgagatcacc cccaacccaa
    28201 gcaacacttt taaacgtact tcaatgtcag ccagcggtcc ttgatgttca aacaaggaat
    28261 ccaccacgga ttttaacgca aagtccgttt tgcgaaacac tcgttcaatc aactgttcaa
    28321 ccgctttggc aaagcgttga ttggcaatgc taaggaaacc acgcagggtg gtttcctcac
    28381 tgagtttgtc gatgtaatcc atcatagtgt caagaattat tgatataatt ccaccacttg
    28441 agcaatgacc gcttggtcgg ttaaaccggt gtatttttcc accgctgccg ccacaccgtg
    28501 atttgcaata aattcggcaa gttcgaccgc ttgtgggtct tcctcattgc gatattgcaa
    28561 tgccatcacc acgcctttga ccaagttttg atacggtaag ccatattcca acgtgccacg
    28621 caacggtttg atcaagcggt cgttttcgct cagtttgcgg attggctcac gccctacacg
    28681 gtttacgtca tcgtttaagt acgggttggc aaaacgtttg aggatttttt cgatatacgc
    28741 tgaatgggct tgtgggtcga agccgtaacg tttaatcagc acagcaccgc tctcttccat
    28801 tgtggctttc acctgagttt tgatttcctc aatcgcaatc gcctctttaa tccatttcac
    28861 gcccgcttgt ttgcccaagt aggcacagat taaatgcccg gtgttcaggg tgaacagttt
    28921 gcgttccaca aacgccatca ggttatcggt taattccatt cctttgatgt ttggaatgtc
    28981 acctttgaac tgggtttgat ccacaatcca ctcgctgaac tcttccaccg tgacttccaa
    29041 cggatcagca gggttcggct cggcaggtgg tacaattctg tccaccgcac tatcaacaaa
    29101 accaaccact ttttcaattt ctgcttgctg ctcggcagtt aaatgctcaa aaattttacc
    29161 tttaaagaaa gttgtgccac gcaccatatt ttcacaggcg ataatgttca gcggttgggt
    29221 gttgccactt tccacacgag ccactaacgc tttggcgaac agtggggcaa taaagccaag
    29281 cacattcggg ccgactgcgg tggtaattaa atcggtgttt ttcacttgct caatcacggc
    29341 attttcgtct ttcgagttaa ttgccgcaat gtttttgaca atttccacac ggctgtcatc
    29401 gcccacgatt ttcacgccgt attgcttgtt ttggttgatt tggtcgattt gagtttggtt
    29461 aatatcggca aaagtcacga aaatgcgagc atcagcaagc aatttgccga taaagccacg
    29521 cccgatatta ccggcaccaa aatggagtgc gttcattgat gattgttcct ctataaaacg
    29581 taggggcggg tcctgtgccc gccctaaatt ttgtaggata atgggcgggc acaggacccg
    29641 cccctacaca acagggtgat tatttagata ataatgctaa cactttttcc acgtcatttg
    29701 ttgaggtcag tgttttcatt gcttgttcat cgtccagtgc gttggtaatt gcacttaaaa
    29761 tttggatatg gtcgttgcct tttgctgcaa taccgaccac cagtttcgcc acgccgtctt
    29821 cttcatcggt gaagcgaacg ccgttcgggt attggcagac cacgataccg gttttcagta
    29881 cgctgtcttt ggcatcagat gtaccgtgtg gcacagctaa gccttcgcct aagtaagttg
    29941 aaaccatttg ctcacgggcg aacattgcat ctacatagct tggttgaaca aagcccgctt
    30001 taactaactg ctcaccggca aagcggatcg cttcttcttt actgttagct tgtaagccta
    30061 aaaagacttg ggctgcttgc aattttaacc cttcggcttg aggttcttcc accgggtttg
    30121 caaaattttg tgtattttgc accgcttgtt gtggctggtg gttagccagt tttgccacta
    30181 agctgtcata gaacgcactg tctaagaagt tagtgagcga taagtgttga gccgttgcca
    30241 cacgttgtgt tgcacgagcg gttaagtctt tgtgggtaat caccagttgt gcgtcttgcg
    30301 gtaagtcgtt gatcgctaag ttttgtactt caatgtttaa acctgccgct tgcacttttt
    30361 tgcgtaacat actcgcaccc atcgcacttg aacccatacc ggcatcacac gctacataga
    30421 ttttgttgat gttaccattt actacgcctt tgctttctgc tttcatcgct tgagtttgtg
    30481 cttgtgcatc ttccaatgag gcttcacctt tttgggtttt gagtaagatt gacgcaatcg
    30541 caaaagtcac ggcacaagaa agaatcacgg aggcaacaac tgcaaagtga gcacccggtg
    30601 cggtcattgc taaaatcgca aaaatagaac ccggggaagc cggtgcgatt aaaccgccac
    30661 ctaataatga gttggttaaa acgccggttg caccgcctgc aatcactgca agaattaagc
    30721 gtgggctcat taacacatac gggaaataaa tttcgtgaat accgcctaag aagtgaatga
    30781 ttgacgcact accagcggtt tgttttgctg tgccacgacc aaagaacata taggcaagca
    30841 agataccaaa gcccggaccc gggttcgctt caatcaagaa cagaatagat ttgcccactt
    30901 cttgtgcttg tgctgtaccc aatggcgtga acacaccgtg attgatcgca ttgtttaaga
    30961 ataggatttt cgccggttca acgatgatag aggttactgg cagaagacca gcttcaacca
    31021 acgcccccac acctgcacct aatgccgcag agatttgggt gacgatacct ccaatgaaag
    31081 caaatgaaag cattgccaaa atcataccga tgatcccgac cgagaagttg tttaccaaca
    31141 tctcaaaacc gctttttact ttgccgtcaa tcgctttatc gaaggttttg atcgcccagc
    31201 cgcctaatgg acctacaatc atcgcaccta agaacatcgg gatttcagaa cccacgataa
    31261 tacccattgt agtaatcgca cctacaaccg caccacgctc accaccgact aatttaccac
    31321 cgctgtaacc gattaacagt ggcagcaagt aagtaatcat cggaccgacc agtttagcaa
    31381 aagtctcgtt cggcaaccaa ccggttggaa taaacagggc ggtgataaag ccccacgcaa
    31441 taaaggcacc gatattcggc atcaccatat tcgataagaa acgcccaaaa ccttggactt
    31501 tcacttttaa attagacata gaaacctcaa aaaaggagag aaaattaggg ttgaaaaaat
    31561 cgtgcttaaa ttagcaccac tttcaaaagt tacaaaaatg tttctctcag aaatgtgaag
    31621 tagatcacat taaatttttt tgggcagatt tgaaaatgtg atctatctca catttttgga
    31681 gtaaagatgg cgtaagcgtc ctcgcttgtg ccaagcataa ttctattttt gcgatctaca
    31741 tcgaaaaaat aaaatgaatt gctaacccta actgttcacc tatattacta tcagcataaa
    31801 ctgcacttta gaggttcata atgagtagaa aatataagtt ttatgagaag agcggagcct
    31861 attttgttag ttttgcaaca gtttattgga ttgatctatt tattcgagaa gattattttc
    31921 aaaccattat ccaatcattg aattattgtc gaaaacaaaa agggatgatt ttatatggct
    31981 actgcattat gccaagccat attcatttat tatttcaagc aaaagaccaa aacccgtcag
    32041 atttaataag agattttaaa acatttacat ctaaatgttg attcagcaaa ttaaaacaaa
    32101 tccccaagaa agtagaaaag aatggttatt atggatgttt actcgggcag ctaaacaacg
    32161 aaataatgtc aagcaatacc aactttggca acatcataac caaccgataa caatctattc
    32221 tcagaaattt tttgatgaaa agctaaacca tattcacaac aaccctatcg taagcggttt
    32281 tgtatgcgag gcatttgaat ggaaatatag cagtgcaaga aattatgcta acaacttacc
    32341 tgttttatta gatattgata tatgtcagtg aacggcacaa gcgtaaacgc ttgcgccatc
    32401 gttacatcta taaattaact agagaactcc atcaatcccg ttactttacc ctcatcattc
    32461 actgcaatta atgagtgaat tttcagctct ttcattaaat cttcggcttt ggctaaaaat
    32521 tcggtatcgt taatggtttt tggattacgg gtcataattt cgtcagcggt tttatttagg
    32581 ctctccgccc cgaatttcgc taatgtgcgg cggatatcac catcggtaat aatgccttcc
    32641 aatttatcgt cttgcataat taccgccacc cccatacgcc cttcgttcat cacagataaa
    32701 cattcggtaa aggtggtgtc taagctggcg gttggcactt tacgcaccat cacatctcgc
    32761 actctgttca ataatttacg accaagactg ccacccgggt ggtagcgggc aaaatcttca
    32821 gggcggaaat cacgggcttt cattagcgca atggctaacg catcgcctaa agccatcgtt
    32881 accaaagtag agctggttgg agctagatta ttcaggcagg cttcacgctc tacaccaata
    32941 tttaaaatca catcggcatt tcgcccaagt gtggagtaag gattacctgt catcgcaata
    33001 attttattgc cgaaattttt caggcttgga atcagtttat tcacatcatc agtttcacca
    33061 ctgttagaaa tcagaattac catatcaatc ggtttcagca ttcccaaatc gccgtgaaaa
    33121 gcctctgtcg ggtgcaagaa aaagctcggt gtaccggtgg aggcaaaagt tgccaccatt
    33181 tttttgccaa ccagccccga ttttccaata ccgccaacaa ccactcgccc ttgacaagcg
    33241 agtatcatat ttaccacctc attaaattct tcagaaagtc gatggtttag cgtgataatc
    33301 tgctgagcat agaggtttaa cgtttctttt gcactgtcta aatgattcat ttttacgccc
    33361 ttatacaact aacagctcaa tccctttcgc tttaatctgt tctctcattt cttgagataa
    33421 tccttcatcg gtaatgatag tatgaatttt ttctaaccca cacacaatat tcggactttt
    33481 acgtccaaac ttactggagt cggcaagtaa gacgatttta tttgccgcat cacacattag
    33541 cgaactgact ttatatactt cattgaaagt cgttaatcca acgtctaaat ctagtccgtc
    33601 tgtgccaata aagagtatgt caaaatttga accaataaaa gcggattcag caaaataacc
    33661 gtgaaatgaa gctgatttct cacgaaatgt accaccggaa atcagtaagt tataggattt
    33721 attgagtttc aaaatctcat tgaaaatagt taaactattt gtcattaaag agaggttatc
    33781 aaatgctttt aaatggggaa tcatttgtaa cactgtgctg cctgcatcaa aaataataga
    33841 gtttccctca ttaattaact ctgcagcttt ttgcccgata gcttgctttt gttgaagatt
    33901 aatgccggtt ttctgtgcaa gcggtttatc gtctttgtcc tctttattga ttttctcttc
    33961 ccgttcaaga atttcttcat caattaagac tacactgcca taagtacgca agactttctt
    34021 atcttcttca agtgaagtta aatctttacg aatggttgca ccagttagct taaaatagtc
    34081 tgccagcacg tccacttcgg ttctaccgtg ctgagctaaa taggctaaaa tttgctgtct
    34141 tctttcaaaa ggtttcattg ttcctccaat ttagcaaaga ttggagcgag ctaatctgtg
    34201 ctaatgctgt tttaattttt caatgacatc tagtagcgat gccattcttt tatcggcaat
    34261 tgaaaatttt tcattttgat aatcggtatt caccaaaaat gtagttaaat tagcggcttt
    34321 ggcggcaatt aagccggtta cactgtcctc aataacaata cactcttgtt tttcaatgcc
    34381 taattttttc actgcactca aatagaccgc agggtgtggc ttgccatatt cttcgtccat
    34441 tgcactgcat cgaatcggga aataatccca cagttttaag cgatcaaaca ccgctttaat
    34501 caccaccggc ggcgatgagg ttgcaacggc taacttgatc tgattagatt ttaagaaatc
    34561 caataattca tataaacccg ttaatgcttt gccgtgttgt tcaatcgcag cacaaccgtg
    34621 tgccaaaatg gcttcactta atgcttggtt ggaaacagat aactgaaatt ttttaatcca
    34681 agtgtcgctc aactcatcaa tccgcttacc tctggtgagt tgttcgcaat cttgctcggt
    34741 gatattaata cctaagtcgg ctaatactgc aatttgagcc tctgcccatt ggggttctga
    34801 atcaatcatt accccgtcca tatcaaagat gacactattg atctgcatat ttattctcca
    34861 ttatctcatt tttaactaaa aaaatattca aaatgaaata aaaaatcgca ttatttttga
    34921 tctgagcaac tatatccgga agatatcgcc acttttaagg caaattgtga acgtttatcg
    34981 cttggaaaga ttttttccgg cacaatttga tcaagagaca aaccaatcac agacgctaat
    35041 ttctgcggtc tggcaaagac ggaaaatcct ttcatacaaa ggctatctac cacttttttc
    35101 tcatcatcaa acgccaacac aataaccgat ttaggattaa aacgaccact attacgccca
    35161 accgttactt tgcctttctt actgatttcc ataaaggctt ggttaaaggc tttaatttga
    35221 agccaaccca aaacggcttg taacagccac attatcattg ccaatataat cagaagtgta
    35281 attgaatcca tcattgctcc ttaataaaaa gccacagagc ctaaacgtac ctgcgatttc
    35341 aaaacaatac gctttcccct gtggcatatt aaccggtgtg gcgaattaac aagcggttta
    35401 ttttagtcat tttttgcaaa ttgttgtaaa aaaatcgaca aatttgaccg cttgtttgct
    35461 tagaacatta cctgaccacc ggtaatatta atagactgtc ctgtgcaata agctgcctct
    35521 tcgccggcat agaattttaa gacattcaac acatcttgat aatcacagcc acgttttaaa
    35581 ggcactttat caatataaac ttgctctact tcgctttccg gaatgccgag ttttgccgca
    35641 tattgcggaa ttaacgattg gaacatcggc gatttcaata aattgcctaa cattaatgaa
    35701 tgcacggtaa tgcctttatc tgccaagtct aatgctaacg attgtgttaa accaacacca
    35761 ccaaatttcg ctgcactata accggaatta tacttactcc ctactttacc cgatttagaa
    35821 ttaatttgaa tgatacgccc tttaataccg tcacgaatca ttaagcggga gaagtgtttt
    35881 gcggttaaga aataaccggt taaattcact tttaccgaca aatcaaaatc gtttaagttg
    35941 aaatcccaaa taggcgatgc tttcgctgtg cctgcactgt aaaccaatac atcggcacgc
    36001 ccgaatacgg tatctgttgc tttagctagt gcttcaacgc ttggttcatc agccgcatcg
    36061 acttgcaccc caatcgcatt tccttcgcct tgtttggcat taatgtcggc tgccactttt
    36121 tgtgcatttt cgccgtttaa gtcagcaacc accacacgat aaccggcatc tgccaagcct
    36181 tcagataaaa aagctccaag tgtttgaccg ccgccaacaa cgatagcaac ttttttttca
    36241 ttgttcataa tgtattcctc taaattttca atttgaaatt atttttaatc cgtcattaat
    36301 cacgcttgat aatcaaggtt gagttctctg ttaagccttg cggcggtgtt ccgataacgt
    36361 gaacgcttcc cggaaattcc gcatcatttg ccccgtcaaa acggaaggta atgtgtccga
    36421 gggcggataa gttttcagat gcaacgctac caactgccgt aatcggataa gctacgccat
    36481 caaattcagc gatatcgcct acttctaaag ggctacttaa ttcagatgga ttatgaatga
    36541 agcagtaatc ttgtaaatct gccggtgcgc cttgtttaaa agtgattaac atatcgtctg
    36601 ttaatgattc ctgagcaaaa ttcccgattt tggtaaaggt cgtttgataa ataatcgtca
    36661 tataaatgtt ctctcgtttt gacaagcggt gcattttgca aaaaattttt caaattccac
    36721 cgcttgtatc gtggttaatt attggtagat gaatgcggaa actgcccacg caatcagcac
    36781 ggtcggtgca ccggttaaaa aacggctgac taatactgac ggtacaccca cccgtacggt
    36841 atcttgtttt gcttccgcca ttgaaagacc cacaggaata aaatcacaag cggcttgagc
    36901 attaatagca aagagtgccg gcagtgctaa atgtggcgga atattgccta aaccgatttg
    36961 cgtaccgact aacacgccga taacttgtgc aatcacggct cccggcccta agaatgggga
    37021 aaggagaggg aaggaacaaa tcaacgccag tgtaactaaa ccgattgggc tgtttgccag
    37081 aggggtcaag ccgtgggcaa tcacatcacc taaacctgac gcaatgatga tcccgattaa
    37141 cgcagaaaca aatgccataa acggtaaaat ggttttaagc accgtatcaa tagtgtctcg
    37201 tccggcttgg aagaataacg ccgccaccga tcccattccc ataccgattt ttgctaataa
    37261 accatcactt tgttcggtga tttttttgct ggagtcatac tcttttgccg ttgctgtcgt
    37321 tgatgaagtt ggttttgctt gctctgttgc cacaggcaca cttccatcgg catcaattaa
    37381 gcgaatattg ttttctttca ccgctgaaac ataaatatct tccacaataa attctgctaa
    37441 agggccggat ttaccggtgg agtgaatgtt aatggttgga atacggcgtt ttgggtaaag
    37501 cccacagcgt aataccccac cgcaatcaat aatcgcaatc ccgatttctt ctgccggcgg
    37561 ttcgccttct ttaaaaccat caaccgcttc ccagccggtt aattccacca atttattaac
    37621 aatcgcaggg cgagtgccgc cggtcatata aagcactttt ttgccttcca caacaggtaa
    37681 tgttaatgga ccaccccaac caccgttgcc tttttcaatg taaatcgctt tgttgctcat
    37741 aatctattcc tctgtatttc agttacttgg ttagtgggat aaatgtactt ctctatctaa
    37801 ggtgatcccc attcgttttt caaagaatgc ggtggtgaag tctgttatcc agccacggaa
    37861 gaaattggta aacataccga ctaataaata actgatggct aaaggcccaa gtggtaagcc
    37921 taaggtggtt aaaccgctgg caatacctaa ataaacgaat aattcacccg ggttgatgtg
    37981 agggaataag ccgttcatag agtggcaact ataagaggct gcggcgtagt agcttggttt
    38041 gtaacgctcc ggtaagaaac gccctaagct taatgtcatc gggttacaga aaacgaaagt
    38101 gccgatcacc ggtaagatca aataacgtga aatagggttt ccggcactac gttgagcaag
    38161 tcgttcaatc cgatcttgcc ctacaaattt aatcagggca ttcattacaa ccaataatga
    38221 aattaagagt ggcaaaatac cgtttatcat accgactaag gtttcaccgc ctttttggaa
    38281 caggccgata aaccattctg ctccgtgtgt aattgcttca atcataccga tctccgtttt
    38341 tcaaaataaa atatttttct ttcatctctg ttatgagatg tgcaaatagt aaattatttt
    38401 tattttaaaa actgtgacag cgatcacaaa atgagaaaca actttcaaaa taaattattt
    38461 tttcatttta aatcaaaaca aaagtgaagt ttgaatatta gatgggggaa aaaaggaagg
    38521 ctaaattaaa aaagctcaaa caataatgtt tgagctttgg ttttattcaa tgacttttgc
    38581 agtatatttg aggtttccga ccgcttgtat cagggcttgg ttatccacac tttcttttac
    38641 caccacagtc gcttttcgct ctttcattga ggctttggtg gaaatcacac cttctactgc
    38701 tcgtagttct ttattgacta aataaacgca gagctggcag tacatttccg gaatatggag
    38761 gacgacggtt ttctctgctt gtttcgtttc aatttgagct gttttttctt gtgcgtagct
    38821 tgaatgaacc aaaccaaaag ccaacagcat cgttgcgata tattttttca ttttattctc
    38881 cgctccacat ttccaaaatg taaggcaata cataagggta agttaaaaag aacatcacca
    38941 ccacagaaac tacccaatac aaaatgacca gggttctgcg ggagaagtat ttggtgcaga
    39001 tgattttgtt ggaaaaattc agcaaccaga agccatagcc aaacgcagcg agcgaaatca
    39061 gcaacatcgg cagttgtaga aactccagtt ggtttagctc catcaaccaa ggggaagaga
    39121 tgccaaacac taaataaacc agtggcccaa tacaacagaa tgtggaggtc accgccgaca
    39181 ctatggcggt cacgcacatt gtgataaatt tatggttata actctttaga gatgaattca
    39241 tagccaaatt ctttcgcctc aaacgagttt aacgggtcac cacctacctc agcataaggg
    39301 tagccaaagt tattcagtgg tgtaagttcc ggttgtggat ataaatcgaa atcacgtccg
    39361 tgattgaagc ctacccagtt catttcccag ttgccgaata agtatttcga gaccgcttga
    39421 acatcagcat catccacgtc ttttttctcg gctaaacgca ttttagcgac atcggcagaa
    39481 tctacaggca cccagccgaa gcctgctaaa tagaactctg cacggcagtg ctgcccgccg
    39541 ctttcattcg ccacgccatc ttttgagcta ccaaatgcgg ttttggaata ttgccccatt
    39601 tttaccgctt gccctaaacg aataccaaac acttcacgag caggaatgtt agaggcacga
    39661 gcaagtgcta caaacaccga gttgatgtcg gtacatttac cttttagcac tttggtggtg
    39721 agaattttct ccacatcgcc atcgccacag cctagcaccg agttatcacg ttccatatta
    39781 tcaacaatcc acttgtggat aagttccgct tttttcaacg gattttgctc gttacccaca
    39841 attttatcgg catattcttt cacaataccg tctgttttaa tgtgagccgt tggttttaaa
    39901 tactccaata catcaacaga ataagtgatt ttctccggca tttggtaatt tgccaacgcc
    39961 ccttgagcca taggctcacg atcttgggtt tggatcacca ttttcacttt tagatcacgc
    40021 tttggtgcat cagtatccca agtggcaaac aaggtttttg caccataagc gttattttct
    40081 gtgatatagg cgtttttgta acttccctca aactctatcg attttaccgt ttgataatcg
    40141 gtatcagacg gcaacggaat ccacaaatta gttgttcctt tcgagccttg tggcacaact
    40201 aaatcataag attgcgtaaa ttcgtaagtg tgggtagtgg tgtggaaatt gttctgttga
    40261 acaggagcag caaaagccgt cgcacagctt gaagccacca aagaggcaat aagtttattc
    40321 attgaaatga tccttattag agtgaaaaaa cagttaaaac ttaacatatt aagtattcta
    40381 actacggatt gaggttgcat attagccaat cgagatggga aaagcaatgg gggagatgat
    40441 tttttagata gtgcaagcgt ccacgcttgt actagaaaca aagctaaaat aatcaatagg
    40501 cacaagcgtg gacgcttgcg ccatcagaga gaataacaag cggtcaaaaa tcctcaaaat
    40561 tttacaaata gattttttgc cctaattttg cactttcaaa tacgctttcg atcactttta
    40621 gcacaaaaat gacgtcttcg agtttgataa ttggctcagc attgtgagcg atggcttggt
    40681 acaaatcatc aaacaagttt tgataactga cttcggcatt tcgatagggc tggcgaacca
    40741 cattaccttc cacttcagtg tgtaaaattc cccagttctc cttttcttcg atattccaat
    40801 ctttgccaat aggttgtacg ccatttttca gcaaatcttc ttgattatcg ggagtttgtt
    40861 tcagataaga gcctaatttt ccgtgtaata caaatgtcgg gcttggttca cgagcatatc
    40921 ggctggcttt cagcaccact ttaaaaccgt tagcataata gagctgaata tcaaagttat
    40981 catcaaccaa tgccccttcg tgctgataac gcacatcagc atacaccgcc tccggcttcc
    41041 cgaaaagata caaagcttgg tcaattaaat gtacgcctaa atcatacact aaacctgtac
    41101 caagctcgcc tgattctttc catactttaa catttttctc tttcgcataa cggtcaaaac
    41161 gaatttcgca atctaccaac tctcccagta aattttgctc cattagcgat tttgccgtcg
    41221 caatatggct gtcccaacgg cggttttggt acacataaag cacctttcct tgccgctttg
    41281 caagttccgc caactcttcc gcctcagcca ctgttgccac taacggtttt tcaactaaaa
    41341 cgtgtttgcc tgcaagcaat gactctttta ccattgcata atgggtttga ttcggtgtgg
    41401 taatcagcac taaatccact tctggcgtta aaagagcgtt gaaatcccgc actgtttctg
    41461 cattcggcaa ccattctgcc gcattgttag aggttcgttc atataccttt acaatattaa
    41521 aacgagcatc ttgtttcaaa aacgggatat gaaaaactcg gctggaaaag ccggcacctg
    41581 cgatggcaat attgatgggt tgcatagaca atcctcataa aattttgatg ttatctgctt
    41641 aaataaacaa gcggtcaaat tttaggaaaa ttttgcaaaa aacagataaa atacgaccgc
    41701 ttgtttaact aatcataggt tttgcaaaca atatcttttt cttcacaaaa catttcactg
    41761 tggtttgcac ggcattgctt ttgtacattt aatttggctc tgcctcggct actatgttca
    41821 ctacgatatt cctgagtaaa tggctttaga gtacaaatgt gaacctcttt ggaatcaagt
    41881 ggatctgaga caccaattat aatcggcata gggataccgg cttgccccgt aggagtaacc
    41941 ggcacgacac agccacttaa tagtatgctt aaacttatta accaataacg catatttttc
    42001 tccttttaaa atgtataata aatcagaacg gagcatcttt atcgttacag taagctctta
    42061 atatcttgcc taatttcttg gtatttttat taaaatttcg gcatttaggg cataccaaaa
    42121 gatgcaactg caaacgcata tgttgttgca aacgaagttt ctcatcacaa gactgtgaaa
    42181 taagttctgt tgcttgcctg catttcattt tgattcctct ctaaaccaat tgcgagataa
    42241 acactcttgt aaacgtaaac gagcacgatg aagtaataca tttaagttag agagagaaat
    42301 ttcacattct tggcaaattt ccgcactact catctccaaa tgttcacgca tcataaatac
    42361 tcgtgcttgc tgggcgggta atttatttaa acaaatatca aaaattgccc aaaattcttt
    42421 ttggtaagtt gattgagcta cccccctcca ttcagttaca tcttgatgtt ccaaccaatg
    42481 atcgtggcga tcgaaaaagg cacttggtga ttcttcttcc tcacatagag aactgactgg
    42541 aatatgtcgt tttcgtgttc tgagtaaatc taaaattttg ttctttaaaa tagcaaaaat
    42601 ccaagtttta agtgcagctt ctcgccgaaa agaggctgaa tgcttatagg cattggttaa
    42661 ggcatcctgt acaacatcct cagctaaatc tttatcggct agttgtaggg tggcaaattt
    42721 aaccatttgc tttctaatct cttctatctg tgtagaagat agactcttca tatatctcct
    42781 ttagggaata cccaataaaa aattacactt attttgtaag aattctagat cagctgagtc
    42841 caaaataata acaacaattc ataaaaaaag gatagttcga tgaaaaaatt aacaattcaa
    42901 gatgttacac ccgaaactat tttctaccaa cgccgtaaaa ttattaccgc gttaggttta
    42961 ggtattgcag cttctgcatt gcctaaagta agtatggcag aaacacaaag acacttgcaa
    43021 tcactacagt ttaaaccgga tccaaataaa gggcttgaac tcactccgga aaacaaagta
    43081 ataggctata acaatttcta tgagtttggc gtggataaaa gtgcaccagc aaaaaatgcg
    43141 gcaaacttca aaaccgatcc ttggacattg gaaattggcg gagaagttga aaatccgttt
    43201 agcttaaatc atcaacaatt gcttaatacg tttccactag aagaacgcat ttaccgtttt
    43261 cgctgtgttg aagcgtggtc aatggttgtg ccgtggatcg gctttgagtt agctcgctta
    43321 attgagatgg caaaaccaac cagtaaagcc aaatatgtgg tctttcacac tttatatgat
    43381 cctgcacaaa tgccggggca aaaaaatcgc ttgctaggcg gtgggattga ttatccttat
    43441 gtagaagctt tacgcttaga tgaagcctta aattcattaa cactactatc cgtcggttta
    43501 tacggtaaaa ccctgccacc gcaaaacggt gctcctattc gcttagtcgt gccttggaaa
    43561 tatggcttta aaagtattaa gtcgattgtg aaaatcacct ttgctgaaaa acgcccactc
    43621 actacttggg aaagtcttgc tccaaatgaa tatggctttt atgctaatgt gaatccaaat
    43681 gttgatcatc cacgttggtc gcaagcatct gaacgagtaa ttggtgcagg cgggttatta
    43741 tccgtcaaac gccaaccaac actaatgttt aatggctacg aaaatgaagt ggcaaattta
    43801 taccgtggct tagatttaaa ggtgaactac taatgctaac ctggttacgt atactcattc
    43861 atcttggctg tttattaccg ctagtttggg tagcacagct attatatacg ggtaatgaaa
    43921 caatattagg tgccgatccg attaaagagc tggagcattt tctcggttat ggtgcaatcg
    43981 tcattttttg tataatgttt ctacttggta ttgccctaca atacctaaag aaaaaccagt
    44041 atcaaatttt acgccgtcca ttgggcttat gggcgtttgt atgggcagct ctacatattt
    44101 caagctatct attgttagaa ttaagccttg atgtaaaatt attttttagc gagcttgccg
    44161 gtcgcccata tttgatttta ggagcagctg cttttttcat tctaagcata atggcaataa
    44221 cttcgctgcc tatgctaaaa cgaaaattag gaacacgctg ggggacgatt caccagtggg
    44281 cttatcctgc attagtattg gcgatggtac attattattg gtcggtaaag agcgtaacat
    44341 tcgatccaat tatcatcgga gtcttagtgc tgtttattgt tgctcataaa atacaggcta
    44401 aatttgcaaa aaaatagcaa aatcacaccg cttgcagcag tcaaactaca tacaaaaaat
    44461 aaggggctgt cctagataac gactaaaac
//
LOCUS       DANLUQ010000003        42569 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.003, whole genome shotgun sequence.
ACCESSION   DANLUQ010000003 DANLUQ010000000
VERSION     DANLUQ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 42569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42569
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.003"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..1604
                     /gene="pckA"
                     /locus_tag="PXB27_000132"
     CDS             <1..1604
                     /gene="pckA"
                     /locus_tag="PXB27_000132"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDL5344968.1"
                     /translation="NRIEQELADLGITNVKEIVRNPSYEQLFEEEMKSDLKGYEKGVL
                     TNLGAVAVDTGIFTGRSPKDKYIVLDETTKDTVWWTSDAAKNDNKPMTQSTWQSLKTL
                     VTDELSNKRLFIIDAFCGASPEHRINVRIITQVAWQAHFVKNMFIRPTEEELKDFKPD
                     FVVMNGSQVTNPNWKEQGLNSENFVAFNITEGVQIIGGTWYGGEMKKGMFSMMNYFLP
                     LKGVASMHCSANVGEEGDVAVFFGLSGTGKTTLSTDPKRKLIGDDEHGWDENGVFNYE
                     GGCYAKTINLSIENEPDIYRAIRRDALLENVVVREDGTIDFDDKSKTENTRVSYPIYH
                     IDNIAVPSKAGHAKKVIFLTADAFGVLPPVSKLTPEQTKYYFLSGFTAKLAGTERGIT
                     EPTPTFSACFGAAFLSLHPTQYAEVLVKRMEAAGAEAYLVNTGWNGTGKRISIKDTRG
                     IIDAILDGSIEKAEMSTLPIFNLAIPTALPGVETHILDPRNTYEDKAQWQAKAEDLAG
                     RFVKNFEKYATNEEGKALIAAGPKL"
     gene            1697..2698
                     /gene="bioB"
                     /locus_tag="PXB27_000133"
     CDS             1697..2698
                     /gene="bioB"
                     /locus_tag="PXB27_000133"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDL5344969.1"
                     /translation="MTTFQLRLKAKDELTPHPTAQYWRKCQVEGLFDLPFMDLVFRAA
                     QVHRENFDANAIQLSTLLSIKTGGCPEDCEYCPQSARYDTGIEKQTMLDVETIVEKAK
                     IAKERGASRFCMGAAWRGPRPKDIKVVSEIINAVKSLGLETCGTFGMLEEGMAEELKQ
                     AGLDYYNHNLDTDPERYNKIIHTRNHEDRMDTLGKVRNAGLKVCCGGIVGMNETRPER
                     AGLIASLANLDPQPESVPINQLIKVEGTPLTNAEDLDWTEFVRTIAVARITMPQSFVR
                     LSAGRTTMPEAMQALCFMAGANSIFYGDKLLTTENPEEDHDRQLMDKLDLYPLQYIA"
     gene            2858..2955
                     /gene="ffs"
                     /locus_tag="PXB27_000134"
     ncRNA           2858..2955
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PXB27_000134"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            2990..3697
                     /gene="gloB"
                     /locus_tag="PXB27_000135"
     CDS             2990..3697
                     /gene="gloB"
                     /locus_tag="PXB27_000135"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HDL5344970.1"
                     /translation="MLQITPIPALKDNYIWAIRNGQDVIIIDPSEHQPALNFIAKNAL
                     NLTAILITHNHHDHTDGVSGIVEQYPNIPVYGPQEVAEFANVIVSPEQHISLFGYDVR
                     IIESGGHTAGHISYLFGYDYLFCGDALFSGGCGRVFTGDYQAQFDALQRFKNLPNSVK
                     AYPAHEYTKSNLKFAEAVLPPSCVLSEYQEQVDLLRSQNKPTLPTTIGLEMQVNPFMQ
                     AVDLAEFIALRQQKDNF"
     gene            3856..4569
                     /gene="uppS"
                     /locus_tag="PXB27_000136"
     CDS             3856..4569
                     /gene="uppS"
                     /locus_tag="PXB27_000136"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005619022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HDL5344971.1"
                     /translation="MNFDPQNMPKHVAIIMDGNGRWAKQKGKLRIFGHQNGVKAVRSA
                     VSFAAKHHIQVLTLYAFSSENWSRPETEVSALMSLFMKALDSEVKQLHKNNIRLKIIG
                     DKSAFSENLQKRIAESEKLTENNTGLILNIAANYGGYWDIVQAAQQLAVHVKSEQLAV
                     EQITPALFQQHLVTENQPQVDLLIRTSGEQRISNFLLWQIAYAELFFSPVLWPDFNDE
                     AFSDAIYAYQQRERRFGGC"
     gene            4593..5462
                     /locus_tag="PXB27_000137"
     CDS             4593..5462
                     /locus_tag="PXB27_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDL5344972.1"
                     /translation="MLKERVLSAIIMIIAVLAAIFWLSPLPLTLVLSAIVVAAMWEWA
                     QFAGFKHPVPRAIVAMVTICLLLFAIVANTDYIRATRFITDETTPILFIGCIWWLIAL
                     VLVATYPNSAKLWEKSVAAKFLFGLATLVPFLIGTLALRFHNYAFDPYQGTYLFLYVC
                     LLVWGADSGAYFAGRAFGKRKLAPKVSPGKSWEGVIGGVLTSSLIAFTFLQLTPANVF
                     GKALATTPFILVSVGMVAISVLGDLAESMFKRQAGIKDSSQLIPGHGGILDRIDSLTA
                     AVPFFATFFFFVL"
     gene            5466..6776
                     /gene="rseP"
                     /locus_tag="PXB27_000138"
     CDS             5466..6776
                     /gene="rseP"
                     /locus_tag="PXB27_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDL5344973.1"
                     /translation="MTSIIAFFILICVLVFVHEYGHFWAARKCGVKVIRFSIGFGKVL
                     FKKKDKQGTEFVFSLIPLGGYVQMWNDETDINAPAQQALNTKSVLQRAFIIIAGPAAN
                     FIFAILAYWVVFIAGIPTVKPVIGEILPNTIASQARIPTELEITKIGHQSIQDWESAS
                     LALVGFVGNKNVPLEGNINGNSTQKYELDLSNWSIDSAKENPLTALGIRPKSAKVTAE
                     IKQVVENSPASQVGIQAGDRILQINQQPFNWFNLVELVQAGKPIELKIEQRGQIKDLV
                     VQPEKKDERYIIGVIPSYEPLADKYRTELKYDILTAFYKSIEKVWSLTQTILQFIGNL
                     ISGDLSIKNLGGPISMAKGAGATAEIGLVYYLSFMALISVNLGVMNLFPLLPLDGGQL
                     VLLAVEAIRGKALSEKIQLKFQQIGFAFVLSLMLFAFANDIIHF"
     gene            6891..9272
                     /gene="bamA"
                     /locus_tag="PXB27_000139"
     CDS             6891..9272
                     /gene="bamA"
                     /locus_tag="PXB27_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HDL5344974.1"
                     /translation="MKKLLLSSLLIANGVVAAPFVVKDIRVEGVQPTTGESIISSIPV
                     RVGQTATDTDVSNVVRHLFSQQRFADVRATREGNTLVIKVAEKPIIGKVEIEGNQAIP
                     KDALEQNLKANLINQGEIFDAAKLEAFKEGLIEHYHSTGRYEAKIDTSVSNNTEGGVN
                     IKLAINEGEVAKAKTIKFEGNNAFSDKKLLENLDIQPDVSWWNIFESSKFEQTAYQKD
                     LETLRDFYLNRGYAKFAIQDTDVQFNDNKTEVALTYKLDEGSQYHVSEMRIIGNTANM
                     DSQLNALLKEFKPGQLFRKSDLTQIEEEIKSILGDAGYGSAKVDIYPQFNEENKTVKI
                     SFVVDAGRRIYVRKIRFEGNGVTADSTLRREMRQQEGAWLSTNKASLGKARLERTGFY
                     ESVDMSMPNVEGTADQVDVVYKIKERNTGSINFGIGYGTKNGISYQAGIKQDNFLGMG
                     STISLNGTRNNYGTSVNLGYTEPYFTKDGVSLGGNIFYEDYDNSDNDSMASYKRQTYG
                     INGTLGFPVNENNSYYLGLGYTHDKIKNAAREYTREKYVKSMNFEFDPAADYYKKIKA
                     DDFDFSLGWNYNSLNRGYFPTEGTTANIGGKITIPGSDNKYYRVSADFRNYYPLNREH
                     KWVISTKASIAYTNGFGGKEVPFYQLYTAGGIGTLRGFSYGAVGPQAIYYNTKSGKFD
                     SMNNDIIGGNALAAASLELITPTPFVSEKYQHNVRTSLFVDAASAWNTKWKKDEYTNL
                     PDYGDFKRFRASAGIAFQWMSPIGPLSFSYAKPIRKYDNDDIEQFQFNIGSSF"
     gene            9352..10143
                     /locus_tag="PXB27_000140"
     CDS             9352..10143
                     /locus_tag="PXB27_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HDL5344975.1"
                     /translation="MKKLFKMTGLAVALASTTGIANAADTIGFVDPSYVLQNHPVLLD
                     ASAKFDKFMKESQEKFAADEKKLADENKTLTAERNKINEDAQKLQNEQKNVEASIKKK
                     VAALEKDAPRLRSKEIQARQNAIQKEANAFQAKVTAIQKREADFAKKAEAFQKRADEF
                     QKRIEQANKENGGINPQEAQKQAVDEVNATIKEIAKSKGYTLVLQPQVALYAEDEGKD
                     ITEAVLDAIVKKHPEIKIEKPATEAQPATEKETKAEETKPEEAKK"
     gene            10143..11168
                     /gene="lpxD"
                     /locus_tag="PXB27_000141"
     CDS             10143..11168
                     /gene="lpxD"
                     /locus_tag="PXB27_000141"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HDL5344976.1"
                     /translation="MANFRLIDLAEHIGGTLRGNADLAIQSIAALDKADSSQITFISN
                     AKYRENLTACNAGAIIVSPADVEFCRADQNLIIVANPYVAYAQLAQYMDSTPKAASEI
                     HPSAVISPEAKLGNNVSVGANAVIESGVELGDDAVIGAGCFVGKNSKIGARTQLWANV
                     SVYHNVQIGADCLIQSSTVIGSDGFGYANDKGQWIKIPQTGGVIIGNRVEIGACTCID
                     RGALDPTVIEDNVIIDNLCQIAHNVHIGFGTAVAGGVIMAGSLKVGRFCQIGGASVIN
                     GHMEICDGAIITGMGMVMRPITEKGIYSSGIPLQTNKEWRKTAALVMNIDEMNKRLKS
                     LEKRFND"
     gene            11197..11655
                     /gene="fabZ"
                     /locus_tag="PXB27_000142"
     CDS             11197..11655
                     /gene="fabZ"
                     /locus_tag="PXB27_000142"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDL5344977.1"
                     /translation="MTTENREPNIIEVTEIMDMLPHRYPFLLVDRVTDYEEGKWLKAI
                     KNVTFNEPCFTGHFPGAPIFPGVLILEAMAQATGVLAVATYGKLSEDELYYFAAIDNA
                     RFKRPVVPGDQLQLEVEFLKEVRGITKFTGKAYVDGKLVCEADLMCARRK"
     gene            11689..12483
                     /gene="lpxA"
                     /locus_tag="PXB27_000143"
     CDS             11689..12483
                     /gene="lpxA"
                     /locus_tag="PXB27_000143"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HDL5344978.1"
                     /translation="MRLIDSTAKISPLAIIEEGAEIGAHVEIGPFSVIGKDVKIGART
                     KIHSNVVINGMTEIGEDNHIFQFASIGEINQDLKYQGEPTKVVIGNRNRIRESVTIHR
                     GTVQGGGVTKIGDDNLFMINTHIAHDCSIGNRCIIANNGTLAGHVTLDDFVIVGGMSA
                     IHQFVVIGSHVMLGGGSMVSQDVPPYVMAQGNHARPFGINFEGLKRRGFDKPTMHAIR
                     KVYKLIYSSGKTLEECLVEIEQIAATEPAIAIFKQFFKRSTRGIIR"
     gene            complement(12521..14521)
                     /gene="mnmC"
                     /locus_tag="PXB27_000144"
     CDS             complement(12521..14521)
                     /gene="mnmC"
                     /locus_tag="PXB27_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HDL5344979.1"
                     /translation="MGKLTFATLTFNDADTPVSEQFDDIYFSTQDGLAESYYVFQEGN
                     QLWEKWQSHPKEAFVIAETGFGTGLNFLAVADKFQQFRAAFPQSKLKRLYFISFEKFP
                     LTASQLADIHQRYPQFATLSENLTACWQPRQIGCQRYHFEQVYLDLWFGEIAENLPQL
                     GDLYSGTIDAWFLDGFSPDKNPEMWNEQLYLQMFRLSANGGSFATFTASSMVRRGLEA
                     AGFAVKKRKGFGKKREMLWGEKPLDSQVSEPNYPYFYAKNETADDIAIIGGGVASFFV
                     ALSLLEKGKQVTLYCKDETLAINASGNLQGAIYPQLSDDDERNVQFYIHSFDYALQRL
                     QQLKSNIEFEHNLSGVVLYAYDEKTAKKLEKMVEQTADDTLLKSCSQAELSEKLGLSV
                     PNSGAFIPQAGWLSPVQLVRNGFTWLEQKGLKIVLNHKVEHLTFSNGMWQWQTRGETV
                     RHQAVVLANGHTLNEFSQANGIPLYPVRGQVSQIPTTSELQKLKCVLCYDGYLTPMSA
                     YGTHCIGASHIRDNAETEFSLSEHWENVAKLQQNVTVCEWTQGIDLSENKAKVGIRAA
                     FRDRVPMVGAVHHFEQQKSQYANLYNQLRRKQAVENAEIFTNLYMVNGLSSRGLTTAP
                     LLGELLASLIVNEPVPISEDIWHNLSPNRAWIRKLLKGSTVA"
     gene            complement(14746..16182)
                     /gene="trpCF"
                     /locus_tag="PXB27_000145"
     CDS             complement(14746..16182)
                     /gene="trpCF"
                     /locus_tag="PXB27_000145"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HDL5344980.1"
                     /translation="MQNIVQDNAQPNQPTILQKIVQDKAIWVAQKEQAFPLSEFQHKI
                     VKSDHDFYAALAKGTHQEPAYILECKKASPSKGLIRAEFDLDSIAQVYKNYAAAISVL
                     TDEQYFQGDFRYIDQVKRQTTQPILCKDFMISAYQVYLARFYNADAILLMLSVVNDET
                     YRELATLAHELGMGVLTETSTEAEFERALALGAKVIGVNNRDLHTLTIDMNRIVRLVE
                     KYQAQISADVCLISESGINDHQQVKNIKPYVHAFLIGSSLMGSADLNNAVRAVIFGEN
                     KVCGLTRVQDVKAVYEQGALYGGLIFAEGSPRQLSLRQAQELVVNAPLRYVGVFQNQA
                     VEFVEKMAKQLELFAVQLHGSEDEQYIAELAEKLGSKTQIWKALSVDVEVENLEFNDN
                     PQIARYVLDSKVGNQQGGTGKTFNWALIPETLKQKALLAGGIGTENIEQALAQGCVGL
                     DLNSGVESAKGVKDLQKLTACFGKIIQA"
     gene            16270..16581
                     /locus_tag="PXB27_000146"
     CDS             16270..16581
                     /locus_tag="PXB27_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HDL5344981.1"
                     /translation="MKSQVIQHLSYLEIALRVNQLWEATPPSPEALANEQPFCVETLT
                     PTQWLQWIFIPRMRAILDANADLPRNFAITPYLEEALKNEGYLQAICEPLLGLEKLLK
                     D"
     gene            16735..17463
                     /gene="truC"
                     /locus_tag="PXB27_000147"
     CDS             16735..17463
                     /gene="truC"
                     /locus_tag="PXB27_000147"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HDL5344982.1"
                     /translation="MTLDILYQDNSLIAINKPAGVLVHRSWLDKHETVFAMQTLRDQI
                     GQHVFPIHRLDRPTSGVLLFALNSETARIMSEQFEQHLVQKTYLAVVRGYLQGSAQID
                     YPLKVILDKIADKFSQEKEAQAAITDYKGLATVEMPYPVGKHQTSRYSLVELSPKTGR
                     KHQLRRHLKHLFHPIMGDSKYGDLHQNRALVEKSGVSRLMLHAHKLQFQHPKNLQKIE
                     IIASLDEQWQRLFAFFDWNFTQYY"
     gene            17481..17645
                     /locus_tag="PXB27_000148"
     CDS             17481..17645
                     /locus_tag="PXB27_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HDL5344983.1"
                     /translation="MDNSLLSLTHEQQQAAVEEIQKLMAQGISAGEAIQIIANQLRDK
                     YQILEKSNNE"
     gene            17638..18114
                     /locus_tag="PXB27_000149"
     CDS             17638..18114
                     /locus_tag="PXB27_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5344984.1"
                     /translation="MNKLWTVLALTITSHYSFAAAEKIVTKDLPAEGKPAVVLQVFDE
                     SEKIAKPIKGNKLFISKKQTRLCWHSINLPVQNKVTIAEAFYAPAAMKLASPGSKINS
                     SADKKNHTIITEINLANNQVLNRCWGFDKTDPVGKYKMEIQINDHIFKGLEFEIVK"
     gene            complement(18151..19002)
                     /locus_tag="PXB27_000150"
     CDS             complement(18151..19002)
                     /locus_tag="PXB27_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HDL5344985.1"
                     /translation="MENQKQAEIELKIMLEQQNVALVENYLNQTSPFPVLAHQTELLG
                     NTYYDTPEQFFATQKMGLRVRSVNQQYEITLKTQGEIVGGLHIRPEYNLALPNSQPDF
                     QTLVEKYQLPFENSEQIAESLQAIFSTDFTRKTWLVQVGMSDIEVALDQGIIKNHFGE
                     EPICELEFEIKQGNLGDLFTLVEAMPKADGMWLSNLSKAQRGYLLGQAVKWEQKIATA
                     MDGENSEKLTQLLADFICVGNESLEVLVRFNQCTQQQFSNWQQARAFVKSKDYLLHNL
                     TNLKTMI"
     gene            19063..20472
                     /locus_tag="PXB27_000151"
     CDS             19063..20472
                     /locus_tag="PXB27_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HDL5344986.1"
                     /translation="MFSKIHHKLNNFVQRGLDNHLRLAVTGLSRSGKTAFITSLVDQL
                     LHINKGGNSHLNLFAPARHGQILSVKRVEQGDLTIPRFEYDKNRQSLENQPPQWPEST
                     TGISEIRLAIRYQRQHSLLRHIKETGTLYLDIFDYPGEWLLDLPLLSLSYKEWSQTQQ
                     TIHKDERAELATPWLQAVKKLDLFAKTDENLLAEFSQQYTDYLLACKAQGMQYIQPGR
                     FVLPTENAKGAPVFQFFPLLDLSEAEWEKLEQSDRNSIFHTLKKRYEHYQKKIVKPFY
                     EDYFSQFDRQVILADCLTPLNYGYNEFMEMKLGLQQLFKHFHYGNRSLFHRLFSSNID
                     KLLFIATKADHITSDQVPNLESLMRQLVQEGGRHAEFDGIETGYLAISSIQATDAVKV
                     TQQDKIIKAIRGIRSSDRKQVTLYPGSVPNRIPDKNYWQFNRFEFDQFEPKQLDYSQA
                     IPHFRMDSVLQFLLADMFE"
     gene            20518..20868
                     /locus_tag="PXB27_000152"
     CDS             20518..20868
                     /locus_tag="PXB27_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5344987.1"
                     /translation="MNTVISQRKPIHWVGSTLDDIREFPDNIKRELGFDLDLVQQGLL
                     PRDFKAMANLGSGVMEIRVKDPSGAFRLVYLAKFKDAIYCLHAFQKKTQKTAPKDIAV
                     IKARYAAVKELENE"
     gene            20861..21184
                     /locus_tag="PXB27_000153"
     CDS             20861..21184
                     /locus_tag="PXB27_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL5344988.1"
                     /translation="MNNIDTKIRHTTEAQGNVFEDLGFPIDEAQKLKSASQQLIETKL
                     MLMNEMSNWIDKNNLKQSEAASILGVSRPRISDMVNGKLEKFTLDTLVTFVAKTGKTI
                     QLQLS"
     gene            21292..21771
                     /gene="smpB"
                     /locus_tag="PXB27_000154"
     CDS             21292..21771
                     /gene="smpB"
                     /locus_tag="PXB27_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDL5344989.1"
                     /translation="MAKKPKVASNTIALNKRARHEYFIEDEIEAGLELQGWEVKALRA
                     GKGNISDSYVIFRHGEAFLFGAMITPLNMASTHIVSDPTRTRKLLLNKRELDSLFGKV
                     NREGYTIVATSMYWKNAWVKVKIGLAKGKKLHDKREDIKDREWQVAKQRIMKNANRG"
     gene            21974..23281
                     /gene="serS"
                     /locus_tag="PXB27_000155"
     CDS             21974..23281
                     /gene="serS"
                     /locus_tag="PXB27_000155"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDL5344990.1"
                     /translation="MIDQNLLRTNLEDVATALKIKRNFILDVERVKTLEEQRKTLQVK
                     TETLQAERNTRSKNIGAAKARGEDISALLAEVDSMGNELDTAKVELDKVQAEIRELLL
                     SVPNLPADEVPLGKDDSENLEVLRWGEPRQFDFEVQDHVTLGENLNGLDFAAGVKLTA
                     SRFVVMKGKLARLHRALSQFMLDLHTEQHGYVETNVPFLVNHDTLYGTGQLPKFGEDL
                     FHTQPLTGQDPNEVQRPFGLIPTAEVPVTNLVRDEIIDEDSLPLRYTAHTPCFRAEAG
                     SYGRDTRGLIRMHQFEKVEMVQIVAPEKSMEALEELTGHAEKVLQLLGLPYRKVLLCT
                     GDMGFGAAKTYDLEVWLPAQNTYREISSCSNMWDFQARRMSARCKAKGDKKTRLVHTL
                     NGSGLAVGRTLVAVLENYQNADGSITVPEVLRPYMGGLEVITA"
     gene            complement(23340..23666)
                     /locus_tag="PXB27_000156"
     CDS             complement(23340..23666)
                     /locus_tag="PXB27_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5344991.1"
                     /translation="MVSVDFKNNLLEFIFYGLFSEHTQTNIINGLQFYPKAGVAFRKD
                     LRFRKRAVYQREIMVFYQLDKIDEPKKVTIIAFRLGRMKNPIRLDTVQNRRVVTEIFM
                     NYPEKD"
     gene            complement(23659..23871)
                     /locus_tag="PXB27_000157"
     CDS             complement(23659..23871)
                     /locus_tag="PXB27_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5344992.1"
                     /translation="MSIVIVDTDFVVHKEDEEWTTEEIKMIEEHLLEEQSAQYQVKRE
                     VPKQFYADIAERLSRGDLTAWEQLDG"
     gene            24058..26643
                     /gene="leuS"
                     /locus_tag="PXB27_000158"
     CDS             24058..26643
                     /gene="leuS"
                     /locus_tag="PXB27_000158"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDL5344993.1"
                     /translation="MQQQYNPSEIEPKVQRYWAENKVFKAVKDVTKEKYYCLSMLPYP
                     SGKLHMGHVRNYTIGDVVSRYQRMIGKNVLQPMGWDAFGLPAEGAAVKNNTAPAKWTY
                     ENIEYMKGQLKMLGFSYDWDREVTTCRPEYYKWEQWFFTELYKKGLVYKKTSTVNWCP
                     NDETVLANEQVHEGCCWRCDTPVEQREIPQWFIKITDYAEELLNQLDNLPQWPDQVKT
                     MQRNWIGRSEGVEITFKIAGSNVELPVYTTRPDTFFGVSYVAIAAAHPLAELAAQNNP
                     ALAEFIREAKNTKVAEAELATMEKKGMATGLFAIHPLTGKEVPVWVANFVLMHYGTGA
                     VMAVPAHDERDFEFAQKYDLQINQVIQPLDGSEWDFSKAAYTEHGKLINSAEFDGLEF
                     DVAFTAIADKLEAMGVGKRQVNFRLRDWGVSRQRYWGAPIPMMTTEEGEVVTVPLQDL
                     PVILPEDVVMNGVQSPIKADPEWAKTTYNGKPALRETDTFDTFMESSWYYARYTCPQY
                     HEGMLDSDEANYWLPVDQYIGGIEHATMHLLYFRFFHKLLRDAGILNSDEPATKLLCQ
                     GMVLADAFYYTSPTNERIWVSPTQVTLERDEKGRIIKATDPQGRELVHSGMTKMSKSK
                     NNGIDPQEMVEKYGADTVRLFMMFASPAEMTLEWQESGVEGAKRFLGRVWNLVYSYAQ
                     NPATTALDVNALDKDQKELRRDVHKTIAKVSDDIGRRQTFNTAIAAVMELMNKLTKAP
                     LETEQDKAVMAEALSAVVRMLYPITPHICFELWQVLGNEQTIDFAPWVQADESAMVED
                     EKLVVVQVNGKVRGKITVSATATEDEVKAIAKADPNVAKFLDGQQIVKEIYVPFKMLS
                     FAVKA"
     gene            26843..27331
                     /locus_tag="PXB27_000159"
     CDS             26843..27331
                     /locus_tag="PXB27_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5344994.1"
                     /translation="MIKKLSLIAVFALTACGWHFKNNEVLPESFRTLTFESADQHSEM
                     SRILRNQLQLSDVKLVPSTANVAKLRLVSTSTDSKVVSVFKQAREAEKILTLNVDAIA
                     DIPNKGQYPLEVSVHRTFFDDSRAALAKSAEKEMIMKDMYEQASRQLIIKMAGLKKEL
                     SK"
     gene            27374..28399
                     /gene="holA"
                     /locus_tag="PXB27_000160"
     CDS             27374..28399
                     /gene="holA"
                     /locus_tag="PXB27_000160"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDL5344995.1"
                     /translation="MQKIFPETLQFSLNKGLQPFYLLTGNDLLLVNETKDAIIHTARL
                     NGFDEKKEVEIKNDTNWEELFEAAQSMGLFFARQIMVLNFPDSPTATQFKKLAELCAF
                     IHSDLLFILHLPKFSKTMEKQAWFTQIELNLVQINCQTPELSKLPQWVSHRAKAMNLQ
                     IENEANQLLCYSYEGNLLALKQALQLLQLNATDGKITLTKAQEVVEHSAQFTPFQWID
                     ALFEGKIQRAQRILYHLQNEEVQAVVLLRIVQKELMILLEITRNPTAVVSSEQSLFIG
                     NLRTEFDRLKIWQNRRPFYNQITQRLTYQKLYALIQQLAELERKVKQEFSDDIWQELE
                     RFSAQFT"
     gene            complement(28400..28585)
                     /gene="yacG"
                     /locus_tag="PXB27_000161"
     CDS             complement(28400..28585)
                     /gene="yacG"
                     /locus_tag="PXB27_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HDL5344996.1"
                     /translation="MPETIVNCPTCEKEVVWSKESKYRPFCSERCQIIDLGDWAAEKH
                     SIASEEAELFSEDLEKY"
     gene            complement(28567..29202)
                     /gene="coaE"
                     /locus_tag="PXB27_000162"
     CDS             complement(28567..29202)
                     /gene="coaE"
                     /locus_tag="PXB27_000162"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDL5344997.1"
                     /translation="MTYVVGLTGGIGSGKTTVSDLFSALGVEVIDADVVARQVVEPGT
                     PLLAKIVEHFGEEILTAEKALERTKLRQIVFNNEEEKTWLNNLLHPAIREEMVKKLQE
                     STACYVIWVVPLLIENKLTEYCDRILVVDVSPEIQLERATKRDNSKIETIKNIMAAQV
                     SREERLSYADDVIENNLPFEQGLLLIKEQVQALHYKYLALAKKEKECQKLL"
     gene            complement(29238..29894)
                     /locus_tag="PXB27_000163"
     CDS             complement(29238..29894)
                     /locus_tag="PXB27_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDL5344998.1"
                     /translation="MLILFSLFIAVWLKAEIPRFAIRVNRQIYQETTSLVNIGLTEQQ
                     FLAQSVLQPKQPRSANLFFLLFPLIMLCSESPIIGTIFILLCFLSLLDMCYYLTDIRY
                     LVLIFLLVLWESLADFQHQTFTFTLAFCLLIGLVSQLMFKKEAFGSGDMLLLVALSPL
                     FRLEKMLLLILTASLLGIGYYLAYWLMKKQKLAKLAFIPFISTAMLLVLMLESRGINY
                     "
     gene            complement(29897..31093)
                     /locus_tag="PXB27_000164"
     CDS             complement(29897..31093)
                     /locus_tag="PXB27_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HDL5344999.1"
                     /translation="MTKIYEFHWQAQSRFGQKQRGKRLATNRDMLENLLLAQGYQHIR
                     ISRNFVLSANPKKEEVNSLLSQLALLVNSAMPLKQALSMLLQNCRNIRLYLWLNELIQ
                     HIESGYAFSQSLEKLGEFVTNQEIQLIKMGEQSGKLGIMLTNLAESRAKLEKLAKKVK
                     KILFYPVIVLLISLALSIGLLVLIVPQFAQLYGSKDKTLPLITDILFTLSECLIEQGT
                     VLLGTLLLAVIFYLFFAKKSRLVAKWKMALWAKMPLFSRIIEQSRIIFFSQNIALMLN
                     AHIPLDVALRSFLSEKNDDPILQQEIGTMLNLLQQGYKFSQGINPNVFGAEMAQMLEV
                     GEKSGNVAKMCEHISEMYQQKLDYQIDILSQLLEPMLMLIMGVIVGTIIVGLYLPIFD
                     MGALVE"
     gene            complement(31090..31542)
                     /locus_tag="PXB27_000165"
     CDS             complement(31090..31542)
                     /locus_tag="PXB27_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilB"
                     /protein_id="HDL5345000.1"
                     /translation="MEYSVTDLNSQRFIEISAEQWQKNCNEKQILLRYLAIPLQETEE
                     QLWLGIDDMQNLNACEIFAFIYHKQIEPVLISSDELKFLLNALSPEQSSRQSEIYEEN
                     TAYSLAHSEQVDRNDPIIQILENLFKFCLQHNASDIHFEPQKEKLLRQ"
     gene            complement(31563..32042)
                     /gene="ppdD"
                     /locus_tag="PXB27_000166"
     CDS             complement(31563..32042)
                     /gene="ppdD"
                     /locus_tag="PXB27_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HDL5345001.1"
                     /translation="MQKIVHFRPLGIRQLHRAFTLIELMIVIAIIAILATIAIPSYNS
                     YTQKAALSELLQASASYKSDVEVCIYNSGGLDNCSGGTNGIQANKTSSNTTKYLNSIA
                     VTKGVITVTGKGSLNGYGYTLTPSFSNNTISWTASCSGNDTSLFPANFCSTTVTPNG"
     gene            32223..33602
                     /gene="radA"
                     /locus_tag="PXB27_000167"
     CDS             32223..33602
                     /gene="radA"
                     /locus_tag="PXB27_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDL5345002.1"
                     /translation="MAKAPKTAYVCNDCGAEYSRWMGQCKECKAWNTISEVRLISSKE
                     AAKSDRFSGYAGETSGKVQTLSDISLQEVPRFSSGFNELDRVLGGGVVPGSAILIGGH
                     PGAGKSTLLLQVMCGLAQHLPTLYVTGEESLQQVAMRANHLGLPTANLKMLSETSVEH
                     ICNIADQEKPKIMVIDSIQVMHLSDIQSSPGSVSQVRECASFLTRYAKTRQVAIIMVG
                     HVTKDGTLAGPKVLEHAIDASLLLEGESDSRFRTLRSQKNRFGAVNELGVFAMTEQGL
                     KEVKNPSAIFLSRSEEQTPGSSVMVLWEGTRPLLVEIQALVDHSMLANPRRVAVGLDH
                     NRLSLLLAVLHRHGGLQMSDQDVFVNVVGGVKVTETSADLALILALISSFRNRPLPQD
                     LVIFGEVGLAGEIRPVTSGQERISEAAKHGFKRAIIPHGNVPKKAIKDMQVFTVKKLS
                     DALDILYNL"
     gene            complement(33657..33887)
                     /locus_tag="PXB27_000168"
     CDS             complement(33657..33887)
                     /locus_tag="PXB27_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HDL5345003.1"
                     /translation="MDYVEILGYVAMILVACSFLLKDVIKLRVVNSLGCACFVVYGLL
                     IGSIPVTGLNALVVCINLYYISKGNRPTAAHS"
     gene            complement(34007..34501)
                     /gene="folA"
                     /locus_tag="PXB27_000169"
     CDS             complement(34007..34501)
                     /gene="folA"
                     /locus_tag="PXB27_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HDL5345004.1"
                     /translation="MKISIIVARTINKVIGKDNQMPWHLPVDLAWFRQNTLGKPVIMG
                     RKTYESIGRLLPKRPNIILSRSGFTVEGAYAVQSLEQAVELAKNFANTAEIMIIGGGE
                     LFKQALPLAERLYLTEIQADIEGDTFFEFDEQNWALVEEKWSEIDENNPFRCRFMCLE
                     RKSV"
     gene            34577..34732
                     /locus_tag="PXB27_000170"
     CDS             34577..34732
                     /locus_tag="PXB27_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl kinase"
                     /protein_id="HDL5345005.1"
                     /translation="MATLFLVTSEQIMSKTIVIKFGTSTLTHGTKSLSRPYMLELVKL
                     NYTNSIV"
     gene            34729..35697
                     /gene="proB"
                     /locus_tag="PXB27_000171"
     CDS             34729..35697
                     /gene="proB"
                     /locus_tag="PXB27_000171"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDL5345006.1"
                     /translation="MIVVTSGAAAAGRDYLGHPELPKTLASKQMLAAVGQSQLIRVWE
                     NLFDIYNIHIGQMLLTRADLDDRERFLNARDTLDALLAQKIIPVINENDAVATEEFKV
                     GDNDNLSALVAILAQADQLYLLTDQEGLFSADPRSNPDAKLLSVVEKITPEIRQIAGG
                     SSTGLGTGGMSTKITAADIATRSGVETIIASGSRPNVLVDLANGQNIGSKFLVQSDRL
                     EGRKRWLFAAPQVGEIVVDFGAEQALLQHKSLLPVGVSAIKGEFTRGEVIKIFSQDGK
                     ALALGIARYSSEALLRIKGKKSKEIESLLGYEFGSVAVHSDEMVVY"
     gene            35697..36461
                     /locus_tag="PXB27_000172"
     CDS             35697..36461
                     /locus_tag="PXB27_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HDL5345007.1"
                     /translation="MELGIDVLAILFAVATLAGFIDAIAGGGGLLTIPALMAAGLPPA
                     MALGTNKLQACGGSLSSSLYFIRKKAVNLKQIWLLILCTFLGSAAGTIFVQMINVDFL
                     KTLLPFLILIIGIYFLVSPKIGDEDRKQRISFTLFAFTAAVALGFYDGMFGPATGSFM
                     TLAFILLLGFNLSKAVAHAKVINFTSNFASLIFFTLGGAVMWKIGLIMMVGQFIGGTL
                     GAKMVMTKGKKLIRPMLVTMSFMMVGKMLYEQGIFN"
     gene            36464..37096
                     /gene="nth"
                     /locus_tag="PXB27_000173"
     CDS             36464..37096
                     /gene="nth"
                     /locus_tag="PXB27_000173"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005721589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDL5345008.1"
                     /translation="MNKAKRIEILTRLRNENPKPTTELNYSNPFELLIAVILSAQATD
                     KGVNKATDKLFPVANTPQAILDLGVDGLKEYIKTIGLFNSKAENIIKTCRDLIEKHNG
                     EVPEDRDALEALAGVGRKTANVVMNTAFGHPTIAVDTHIFRVSNRTNFAPGKNVVQVE
                     EKLLKVVPDEFKVDVHHWLILHGRYTCIARKPRCGSCIIEDLCEFKEKTE"
     gene            37293..38543
                     /locus_tag="PXB27_000174"
     CDS             37293..38543
                     /locus_tag="PXB27_000174"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HDL5345009.1"
                     /translation="MDIYLVGGAVRDQLLGLPVKDRDFLVVGATAEQLLAQGFQQVGA
                     DFPVFLHPETKEEYALARQERKNGNGYNGFICDFSPNVTLEQDLIRRDLTINAIAQDE
                     NSTLFDPYGGVQDLENRLLRHVSPAFSEDPLRVLRVARFAARFHSFGFTIAPETLKLM
                     REMVQSGELKHLTAERVWLETQKAFETDNPHVYFDILRLIGALKVLFPELDRLFGVPQ
                     PPQHHPEIDSGKHTLMVLQQAKRLAKKAENPTALLFAALCHDLGKGLTPADILPHHYG
                     HEVKGIQPTRELANRLKIPTEIKDFALLVTEYHTHCHKMAELRPETVIKLFNSLDVWR
                     KPNRFFDFLFACEADARGRLGFEQRNYPQPELAKKYYQAAIEIDVQQVIQDGFEKQAI
                     REELNKRRVYAVRSIKLDSDLINH"
     gene            complement(38644..39579)
                     /locus_tag="PXB27_000175"
     CDS             complement(38644..39579)
                     /locus_tag="PXB27_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDL5345010.1"
                     /translation="MSESKPFPITTGFFAMPLGLGALGLAWLNATPITPYATTIGNII
                     AGTSVILWVALLGCYVYRVFFCFEQIKAEFMHPVQCCLFSLVTMTTMISGDVLYVWDF
                     YLGELLIFLGVIVHLIFLVFIIGGLWKGTQFLPETLHPVHYLPPIAGSFTMASSLSLI
                     GYSEIASLFLGIGIISWLVYEPTMLQRIRLSPLFPIARPSLGIVLAPAFVGASAYFSM
                     GGELDLLAKMLWGYGFLQFCFILRNLKWIAENGFSMGFWAFSFGLAAMARGAIEFYQF
                     EPLSFIGLAGFTIANFGIGLLLIFTLKLWLQQSRA"
     gene            complement(39708..39902)
                     /gene="iscX"
                     /locus_tag="PXB27_000176"
     CDS             complement(39708..39902)
                     /gene="iscX"
                     /locus_tag="PXB27_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005656371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HDL5345011.1"
                     /translation="MKWTDTRMIAENLYDMNPDLDPTTVRFTDMHKWICEMEDFDDDP
                     EASNEHILEAILTIWLEEYE"
     gene            complement(39902..40243)
                     /gene="fdx"
                     /locus_tag="PXB27_000177"
     CDS             complement(39902..40243)
                     /gene="fdx"
                     /locus_tag="PXB27_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HDL5345012.1"
                     /translation="MPKIVFLPHEEFCPEGMVVEAKAGDNLLEVAHEAGVEIHHACDC
                     SCACTTCHVVIREGFDSLNDTSDQEEDMLDKAWGLEIDSRLSCQCIVGEEDLVVEIPK
                     YNLNHANEAAH"
     gene            complement(40262..40627)
                     /locus_tag="PXB27_000178"
     CDS             complement(40262..40627)
                     /locus_tag="PXB27_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 51 family protein"
                     /protein_id="HDL5345013.1"
                     /translation="MSNYVKTEISEQFRTVAVEFDLEDNRIFELGERINTEYDVAYMN
                     GYNWAAFLRAYLSIYRPSLLELLEEDPDVGCYYVTYPLSEKSKEKARELKKIIVYLVE
                     NEEEILNFIGEHLDNIEWD"
     gene            complement(40637..42490)
                     /gene="hscA"
                     /locus_tag="PXB27_000179"
     CDS             complement(40637..42490)
                     /gene="hscA"
                     /locus_tag="PXB27_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HDL5345014.1"
                     /translation="MALLQISEPGQTAAPHQHKLAVGIDLGTTNSLVATVRSGQTQVL
                     LDEQERALVPSVVHYAETEKTVGIEAFAKAALDPKNTVISAKRLIGRSLADVQQRYQN
                     LPYQFVASENGLPLIQTAQGNKSPIEVSADILSHLAQFAEKRLAGELSGVVITVPAYF
                     DDAQRQSTKDAARLAGLNVLRLLNEPTAAAIAYGLESGQEGVIAVYDLGGGTFDLSIL
                     RLRKGVFEVLATAGDTALGGDDFDHLLAEWIAEQANYKPQTASEQRELLTLATQTKVA
                     LSQAVEFEVKFANWIGVVSRSQFNELIQPLVKRSLMTCRRALKDAGVEAEEVREVVMV
                     GGSTRVPFVREQVGEFFGKTPLTSIDPDKVVAIGAAVQADILVGNKPDSDMLLLDVVP
                     LSLGIETMGGLVEKIIPRNTTIPVARAQEFTTGKDGQTAMTIHVLQGERELVEDCRSL
                     GKITLRGIPAMAAGVAQVRVTYQVDADGLLNVTAMEKSTKVQASIQIKPSYGLTDEEV
                     TQMIKSSMANAKEDMEARQLVEQRVEADRVIDSVVTALKEDGSAVLSVEEFQAIEAQL
                     ARLIELKEGTDRFAIQQGIKEVDLATQEFAARRMNLSIQKALAGKKMDDLA"
BASE COUNT        13021 a         8943 c         8480 g        12125 t
ORIGIN      
        1 taaatcgtat tgaacaagaa cttgcagact taggcattac aaatgtaaaa gaaatcgtgc
       61 gtaaccctag ctatgagcaa ctgtttgaag aagagatgaa atcggatctt aaaggttatg
      121 aaaaaggcgt cctaacaaat ttaggcgcag tagcggtaga taccggcatt ttcacggggc
      181 gttcgccgaa agataaatat atcgtattag atgaaacgac aaaagatacc gtctggtgga
      241 catctgatgc agctaaaaat gataataaac cgatgactca atctacttgg caaagtttaa
      301 aaaccttagt gaccgatgag ttatctaata agcgtttatt tattattgat gctttctgtg
      361 gtgcaagccc tgagcatcgc atcaatgtgc gtattattac ccaagttgcg tggcaagctc
      421 actttgtgaa aaatatgttt attcgtccaa cagaagaaga attaaaagat ttcaaaccgg
      481 attttgtggt aatgaacggt tcccaagtaa ctaacccgaa ctggaaagag caaggcttaa
      541 attcagaaaa ctttgtggca tttaatatca cagaaggtgt tcagatcatt ggcggtactt
      601 ggtacggtgg tgagatgaaa aaaggtatgt tctctatgat gaactacttc ttaccattaa
      661 aaggtgtcgc ttcaatgcac tgttcagcca atgtgggcga agaaggtgat gtggcggttt
      721 tctttggttt atcaggaacc ggtaaaacga cactatcaac cgatccgaaa cgcaaattga
      781 ttggtgacga tgaacacggt tgggacgaaa acggcgtatt taactatgaa ggtggttgct
      841 atgcgaaaac catcaacctt tctattgaaa acgagccgga tatttatcgt gcaatccgcc
      901 gtgatgcgtt attagaaaac gtggttgtgc gtgaagatgg cactattgat tttgatgata
      961 aatcaaaaac agaaaatacc cgtgtgagct atccgattta tcatattgat aatattgccg
     1021 tgccgtcaaa agcaggacac gctaaaaaag tgattttctt aacggcagat gcattcggcg
     1081 tattgccacc ggtgtctaaa ttaacgcctg aacaaactaa atactacttc ttatcaggct
     1141 ttaccgcaaa attagcaggt acagagcgtg gtattactga gccaacccca actttctctg
     1201 cttgtttcgg tgcggcgttc ttatcactcc acccgacaca atatgctgaa gtgttagtga
     1261 aacgtatgga agcagccggt gcggaagcct atttagtgaa taccggttgg aacggcacag
     1321 gtaaacgtat ttcgattaaa gatacacggg gtattattga tgccatctta gacggctcaa
     1381 ttgagaaagc ggaaatgagt acattaccaa tcttcaattt agcaattcca actgcgttac
     1441 cgggtgtaga aacccacatt ttagaccctc gtaacactta tgaagacaaa gcacaatggc
     1501 aagctaaagc agaagattta gctggcagat ttgtgaaaaa ctttgaaaaa tatgcgacca
     1561 acgaggaagg caaagcatta attgctgctg gtccgaagtt ataagttgta attataagtt
     1621 ataatcgcta aaaaattagt ttaggactaa accttgaatg aggtttagtc cttttgtata
     1681 aaggagaagt acaataatga caacattcca attaaggctg aaagccaaag atgagcttac
     1741 cccacaccca acagcacaat attggcgaaa atgccaagtg gaaggactat ttgatctgcc
     1801 gtttatggat ttagtgttta gagcggcaca agttcaccgt gaaaattttg atgctaatgc
     1861 gatccagctt tcaacgttac tctcaattaa aacaggaggc tgtccggaag attgcgaata
     1921 ttgcccccaa tctgcccgct atgatacggg aatagaaaag caaacaatgc ttgatgtaga
     1981 gacgattgta gaaaaagcta aaattgccaa agagcgtgga gcaagccgct tttgtatggg
     2041 ggctgcgtgg cgaggaccta ggcctaaaga tattaaagta gtctcagaaa tcatcaatgc
     2101 ggtgaaatca ttggggctag aaacctgtgg tactttcggt atgcttgaag agggaatggc
     2161 agaagaatta aagcaagcag ggctggatta ctacaaccat aatttagata ccgaccctga
     2221 acgctataac aaaattatcc atacccgaaa tcacgaagat cgaatggaca ctttaggtaa
     2281 agttcgcaat gccggcttga aagtttgctg tggcggtatt gtgggaatga acgaaacccg
     2341 ccccgagcga gcaggcctaa ttgcaagcct cgctaacctc gatcctcaac ctgaaagcgt
     2401 accgattaat cagttgatta aagtagaagg tacaccgctt accaatgccg aagatttaga
     2461 ctggacagaa tttgttcgta ccattgcggt agcaagaatt actatgcctc aaagctttgt
     2521 ccgactttct gctggcagaa ctactatgcc ggaggcaatg caagccctct gttttatggc
     2581 aggagcaaat tctattttct atggcgataa attacttact acagaaaatc cggaagaaga
     2641 tcatgatcgc caattaatgg ataaattaga tttatatccg ctacaatata tagcctgagt
     2701 gccaatgatt ttcgcctgaa tgaaaattaa agaatcaatc gttattttac gcaaatttct
     2761 aacataaaat aaccgcttgc aaaattcgtt cttgtcttat ttttaaattt atctataatg
     2821 tgaggcctta cgcaagcttg cgtaattcga tggggcgtta ttggttcctc gcaatggtgt
     2881 tcagcttact tgatcaggtt cggaagaaag cagtctgggt tgaaatttcc gtgtgccgag
     2941 ggaggctggt aatgccccac ccttttttca gttctatcag gagaacgtta tgctccagat
     3001 cacccccatt ccggcattaa aagataatta catttgggca atccgtaacg ggcaagatgt
     3061 gattattatt gatccatcag aacatcagcc agcattaaac tttattgcaa aaaatgcgtt
     3121 aaatttaacc gctatcttga ttacccataa tcaccacgac catactgatg gggtgagtgg
     3181 tattgttgag caatatccga atatccctgt ttatggtccg caagaagtgg ctgaatttgc
     3241 caatgtgatt gtctcgcctg aacaacatat cagcctgttt ggttatgatg taaggattat
     3301 tgaaagtggt gggcataccg cagggcatat cagttattta tttggctatg attatctgtt
     3361 ttgtggcgat gcgttattct ccggcggttg cgggcgggtt ttcaccggtg attaccaagc
     3421 ccaatttgat gcgttacaac gctttaaaaa tctgcctaac tcggttaaag cctacccggc
     3481 tcacgaatac accaagagca atttgaaatt tgcagaagcg gttctaccac caagctgtgt
     3541 attatcggaa tatcaagaac aggttgatct tttacgttcg caaaacaaac cgactttgcc
     3601 aaccactatt ggcttggaaa tgcaggtgaa cccgtttatg caagcggtcg atttagccga
     3661 atttattgca cttcgtcagc aaaaagacaa tttttaacct catatttcta attaagcatt
     3721 aaagcctctc actttaatgc tttttggtta aataaaacct ttgcaaacat cgcttgtcaa
     3781 ttccactctg gcttatccta tggtatagtt acgactattt ttagcttaat ttattatttt
     3841 agtgagtaaa attctgtgaa cttcgatcct caaaatatgc ctaagcacgt tgccattatt
     3901 atggatggca atggcagatg ggctaagcag aaaggaaaat tacgcatttt tggtcatcag
     3961 aatggtgtaa aggcagttcg ttctgcagtt agttttgcag caaagcatca tatccaagtt
     4021 ttaaccctct acgcatttag cagtgagaat tggagcagac cagaaactga agtttcagct
     4081 ttaatgtctc tgtttatgaa agctttagat tctgaagtta aacaattaca taaaaataat
     4141 attcgcttaa agattatagg cgataaatcc gcctttagcg aaaatttaca aaaacgcatt
     4201 gccgaatcgg aaaaattaac cgaaaacaat actgggctaa tcttaaacat tgctgccaac
     4261 tatggtggtt actgggatat tgttcaagct gctcaacaac tagcagtgca cgttaaatct
     4321 gaacaacttg ctgtggagca gattacacca gcattgtttc aacaacattt agtgacagaa
     4381 aaccagccgc aagtggattt attaattcga accagtggtg agcagcggat cagcaatttt
     4441 ctattatggc aaattgctta tgccgagctg ttttttagcc cggttttatg gcctgatttt
     4501 aatgatgagg cttttagtga tgcaatttat gcttatcagc aacgtgaacg ccgattcggt
     4561 ggttgttaat ataaaaagaa taaggaaata caatgcttaa agaacgtgta ctttcagcga
     4621 ttattatgat tattgccgtg ctggctgcca tattttggct ctcaccactt cctttaactt
     4681 tagtgttatc ggcaattgtt gtggctgcaa tgtgggaatg ggctcaattt gccggcttta
     4741 aacaccctgt gccacgtgca attgtggcaa tggtgacaat ctgcttatta ctttttgcga
     4801 tcgttgcgaa tactgattat atccgagcca cccgttttat tactgatgag actaccccta
     4861 tcctctttat tggctgtatt tggtggttaa ttgcccttgt cctagtggca acttacccca
     4921 actcggcaaa actgtgggaa aaatctgttg cagctaaatt cctatttgga cttgctacct
     4981 tagtgccgtt tttaattggc acgctcgcat tgcgtttcca taattatgca tttgatccat
     5041 accaaggaac ttatttattc ctttacgtct gtttattagt ttggggagct gattcaggtg
     5101 cttattttgc tggcagagcc tttggcaaaa gaaaacttgc cccaaaagta tctccgggta
     5161 aatcgtggga aggcgtaatt ggtggcgtac tcacctctag tttgattgcc tttacctttt
     5221 tacaacttac accggcaaat gtattcggca aagcattagc cactacccca tttattttag
     5281 tttcggtcgg tatggttgct atttcagtct taggcgattt ggcagaaagt atgtttaaac
     5341 gccaagcagg cattaaagac agtagccagt taattcctgg gcatggaggt attttagatc
     5401 gtattgatag tttaaccgct gccgttccgt tttttgctac attcttcttt tttgtactat
     5461 agaatatgac gtccatcatt gcatttttta ttttaatttg cgtactggtt tttgtgcatg
     5521 aatacgggca cttttgggct gctcgcaaat gcggggtaaa ggtgatccgt ttttcgatcg
     5581 gatttggtaa agtcttattt aagaaaaaag ataaacaagg aaccgaattt gttttttcac
     5641 ttattcctct gggcggctat gtacaaatgt ggaatgatga aacggatatc aacgctccag
     5701 ctcaacaagc tcttaacaca aaatctgtat tgcaacgtgc atttattatt atagccggtc
     5761 ctgctgcaaa ttttattttt gcgattttgg catactgggt cgtttttatt gccggtattc
     5821 caactgttaa acctgttatt ggggaaattt taccaaacac tatcgcctcc caagcaagaa
     5881 ttcctaccga attggaaatt acaaaaatcg gtcatcaatc tatccaagat tgggaaagtg
     5941 catctttagc actagttggt tttgtgggca ataaaaatgt accgttagag ggaaatatca
     6001 acggcaactc aacacaaaaa tatgaactcg atttatctaa ctggagtatt gactcagcca
     6061 aagaaaaccc attaaccgca ttaggtattc gccccaaaag tgcaaaagtc accgctgaaa
     6121 ttaaacaagt tgtcgaaaat tctcctgcaa gccaagtggg tattcaagca ggtgatcgta
     6181 ttcttcaaat taaccagcag ccatttaatt ggttcaattt agttgaactg gtacaagcag
     6241 gtaaacctat tgaacttaaa attgaacaac gaggtcagat taaagacctt gtcgtccaac
     6301 cagaaaaaaa agacgaacgc tatattatcg gggtaatacc aagttatgag cctcttgcag
     6361 ataaatatcg taccgaatta aaatatgata ttcttaccgc attttataaa agtatcgaaa
     6421 aagtgtggtc tcttacccaa accatactcc aatttatcgg taatttaata agcggtgatt
     6481 tgtccattaa aaatttaggt gggccaattt caatggcaaa aggagccggt gcaacagctg
     6541 aaatcggctt agtttattac cttagtttta tggctctaat cagtgtcaat ttgggggtaa
     6601 tgaacttatt tccactttta cccttagatg gtgggcaact tgttttattg gctgttgaag
     6661 caattcgtgg aaaagcatta tcagaaaaaa tacaactcaa attccaacaa attggatttg
     6721 cattcgtgct gagtttaatg ttattcgcct ttgccaacga tataattcac ttttaacaag
     6781 cggttaaaaa aggtgacttt tttgcaaaaa gcaccctgac atcaaaatcc ataatatgga
     6841 tttaaactat attcttaata attttgcaaa acgatcatag gataatttca atgaaaaaat
     6901 tattactttc ctctctttta attgcaaacg gtgtagttgc tgcacctttc gtagtaaaag
     6961 acattcgtgt tgaaggtgtg caacctacaa caggcgaatc tatcatttca tctattccgg
     7021 ttagagtagg tcaaacagca acggatactg acgtatctaa tgtcgtccgc catctattca
     7081 gccaacaacg ttttgcagat gttcgtgcaa cacgagaagg caatacgctt gtcatcaaag
     7141 tcgctgaaaa accgattatt ggcaaggtag agatcgaagg taaccaagcg attcctaaag
     7201 atgccttaga gcaaaattta aaggcaaact taattaacca aggtgaaatt tttgatgctg
     7261 ccaagcttga agcttttaaa gaaggactaa tcgaacatta tcactctacc ggccgttatg
     7321 aagccaaaat tgacacatct gtttcgaata acaccgaagg tggcgttaat attaagttag
     7381 cgattaatga aggtgaagtt gctaaagcca aaacaattaa gtttgaaggt aataatgcct
     7441 tctctgataa aaaattatta gaaaacttag atattcaacc tgatgtttct tggtggaata
     7501 tttttgaaag tagcaaattc gaacaaaccg cctatcaaaa agacttagaa acattaagag
     7561 atttttattt aaatcgtggc tatgcaaaat ttgctatcca agataccgat gttcaattca
     7621 atgataataa aacggaagtt gcattgacct ataaattaga tgaagggtca cagtaccatg
     7681 tcagtgaaat gcgcattatc ggtaatacag ccaatatgga tagccagctc aacgccctac
     7741 taaaagaatt caagcctggt caattattcc gtaaatcaga ccttacacaa attgaagaag
     7801 agattaaatc tattttaggg gacgcaggtt atggttcagc taaagtagat atttatcctc
     7861 aatttaatga agaaaataaa accgtaaaaa tcagttttgt tgtggatgcc ggtcgtcgta
     7921 tttatgttcg taaaatccgt tttgaaggta atggtgtcac tgcagattca acattacgtc
     7981 gtgaaatgcg ccaacaagaa ggtgcttggc tttccacaaa caaagcttct ctaggtaaag
     8041 ctcgtttaga aagaaccggt ttctatgaaa gtgttgatat gtcaatgcca aatgtagaag
     8101 gtacagccga tcaagttgat gtagtgtata aaattaaaga acgcaatacc ggtagtatta
     8161 actttggtat cgggtacggc acgaaaaacg gtatcagcta tcaagcagga attaaacaag
     8221 ataatttctt aggtatgggg tcaactatca gtctaaatgg tacccgtaat aactacggta
     8281 ccagcgttaa cctaggctat accgaacctt actttactaa agatggtgta agtttaggcg
     8341 gtaatatttt ctatgaagat tatgataact cagacaatga ttcaatggct tcatataaac
     8401 gccaaaccta tggtattaac ggcacattag gcttcccggt aaatgaaaat aactcatact
     8461 acttaggttt aggctatacg cacgataaaa ttaaaaatgc agcacgtgag tacactcgtg
     8521 agaaatatgt aaaatcaatg aactttgagt tcgatccagc tgccgattat tacaaaaaaa
     8581 tcaaagctga tgattttgat ttttctctcg gttggaacta caacagcttg aatagaggct
     8641 atttcccgac tgaaggaact accgcaaaca ttggcggtaa aatcacaatt ccaggctctg
     8701 ataataaata ttaccgagtc agtgcagact ttagaaatta ttacccgtta aaccgtgagc
     8761 acaaatgggt aatttcaacg aaagctagca ttgcttatac taatggattt ggtggtaaag
     8821 aagttccgtt ctatcagctt tatactgccg gaggtattgg tacactaaga ggtttctctt
     8881 acggtgcagt agggcctcaa gcgatttact ataatacaaa atccggcaaa tttgatagca
     8941 tgaataatga tattatcggt ggtaatgcgt tagctgcggc aagcttggaa ttaatcacac
     9001 caacgccatt tgtgagcgaa aaatatcagc acaatgtaag aacctctctc tttgttgatg
     9061 ctgcaagtgc atggaataca aaatggaaga aagatgaata tacgaacttg cctgactatg
     9121 gtgatttcaa acgtttccgt gcttccgccg gtattgcatt ccaatggatg tctccaatcg
     9181 ggccattatc attctcttat gcgaaaccaa ttcgtaaata tgataatgat gatattgaac
     9241 aattccagtt caacattggt agttcattct aattaaccac aagcggttaa taaatgtaaa
     9301 aaatttgcaa cttagccgct tatttcaaaa ctgttcatac aaggaaaaca aatgaaaaaa
     9361 ttatttaaaa tgactggctt agccgtagct ttagcctcta caaccggaat cgcgaatgct
     9421 gcagatacta ttggtttcgt agatccaagc tatgtattac aaaaccaccc tgtattatta
     9481 gatgcttcgg caaaatttga taaattcatg aaagaaagcc aagaaaaatt tgctgctgat
     9541 gaaaaaaaat tagcagatga aaataaaacc ttaacggctg agcgcaataa aattaatgaa
     9601 gatgcgcaaa aattacaaaa tgaacaaaaa aatgtagaag cttctatcaa gaaaaaagtg
     9661 gcagcattag agaaagatgc gcctcgctta cgttcaaaag agattcaagc acgtcaaaat
     9721 gcgattcaaa aagaagcaaa cgcattccaa gctaaagtaa cagcaattca aaaacgtgaa
     9781 gctgattttg caaaaaaagc agaagccttc cagaaacgtg cagatgaatt ccaaaaacgt
     9841 attgaacaag cgaataaaga aaatggtggc attaacccac aagaagcgca aaaacaagcg
     9901 gttgatgaag taaatgcaac cattaaagag atcgcaaaat cgaaaggtta cactttagta
     9961 ttacaaccac aagtagcact ttatgctgaa gatgaaggta aagatattac tgaagcagta
    10021 cttgatgcta ttgttaaaaa acatccggaa attaaaatag aaaaacctgc aactgaagct
    10081 caaccagcta cagaaaaaga aaccaaagct gaagaaacta aaccagaaga agcgaaaaaa
    10141 taatggcaaa tttccgttta attgatttag cggagcatat cggcggtact cttaggggta
    10201 acgccgattt ggctattcaa agtattgctg ctttagataa agccgacagc tcgcaaatta
    10261 cctttatctc aaatgcaaaa tatcgggaaa atttgaccgc ttgcaatgct ggagctatta
    10321 tcgtcagccc ggcagatgta gaattttgcc gagcagatca aaatcttatc atcgttgcca
    10381 atccgtatgt tgcttatgct caactggctc aatatatgga ttctactccg aaagcagcga
    10441 gcgaaatcca cccaagtgcg gtgatttcac ctgaagctaa actgggtaat aacgtatcgg
    10501 taggggcaaa tgccgtgatt gaaagtggcg tagaattggg cgatgatgcc gtgatcggtg
    10561 caggctgttt tgtgggaaaa aacagtaaaa tcggggcaag aacccaactt tgggcaaatg
    10621 tttcggtgta ccacaatgta caaatcggag ctgattgctt gattcaatcc tcaacggtta
    10681 tcgggagtga tgggtttggc tatgcgaatg acaaagggca atggattaaa atcccacaaa
    10741 ccggtggtgt gattatcggt aatcgtgttg aaattggtgc ttgcacttgt attgaccgag
    10801 gtgcattaga tccaaccgta atcgaagata atgtgattat tgataacctt tgccaaatcg
    10861 cccataatgt acacattggt tttggcactg ccgttgccgg cggcgtcatt atggcaggca
    10921 gtctaaaagt tggtcgcttt tgccaaattg gtggagcaag tgttatcaac ggtcatatgg
    10981 aaatctgtga tggtgccata attaccggaa tgggtatggt tatgcgccca attacagaaa
    11041 aaggaattta ctcatcaggt attccattac aaaccaataa agaatggcga aaaaccgcag
    11101 cacttgtaat gaatattgat gaaatgaaca aacgcttaaa atcgttagaa aaacgtttta
    11161 acgattaatt ttattagagg tcttaaagtg acagaaatga caactgaaaa tcgtgagcca
    11221 aatattattg aagtaaccga aattatggat atgttaccgc atcgctaccc tttcctgtta
    11281 gttgatcgcg taaccgatta tgaagaaggg aaatggttaa aagccattaa aaatgtaacc
    11341 ttcaatgaac cttgttttac cgggcatttt cctggcgcac caatcttccc aggagtattg
    11401 attctggaag caatggctca agcaactggc gtattagccg ttgccactta tggtaagtta
    11461 agtgaagacg aactctacta ttttgccgca attgacaatg ctcgctttaa gcgaccggtt
    11521 gtgccgggcg atcaacttca acttgaggtt gaatttctta aagaagtacg tggtattact
    11581 aaatttaccg gtaaagcata cgttgatggc aaattagtct gtgaagcaga tttaatgtgc
    11641 gctcgccgta aataattcga tataagccca aactagaggg aacaaactat gcgtctaatt
    11701 gattcaacag caaaaattag cccgcttgcc atcattgaag aaggtgctga aattggcgct
    11761 cacgttgaaa ttggtccatt ttcagtcatc ggtaaagatg tcaaaatcgg ggctagaacg
    11821 aaaattcact caaatgttgt cattaacggc atgactgaaa ttggtgaaga taaccatatt
    11881 ttccaatttg ccagcattgg tgaaatcaac caagatctaa aataccaagg tgaaccaacc
    11941 aaagtggtga tcggtaatcg taaccgtatt cgtgaaagtg ttactattca ccgaggtacg
    12001 gtacaaggcg gtggcgtgac gaaaatcggt gatgataact tatttatgat caacacccac
    12061 atcgcacacg actgctctat cggtaaccgt tgtattatcg ccaataatgg tacgctagca
    12121 ggtcacgtta ccctagatga tttcgtgatt gtgggaggaa tgtccgccat tcaccaattc
    12181 gttgtgattg gttcacacgt tatgcttgga ggcggttcaa tggtcagcca agacgtaccg
    12241 ccttatgtca tggcacaagg taaccacgca cgcccatttg gtattaactt tgaagggtta
    12301 aaacgtcgtg gctttgataa accaactatg cacgctattc gcaaagtcta taaactgatt
    12361 tactccagcg gaaaaacatt ggaagagtgc ctagtagaaa ttgagcaaat tgcagcaaca
    12421 gaacctgcga ttgctatttt taaacaattc ttcaaacgtt ctacccgtgg tattattcgt
    12481 taataaacga aaccccgaaa tcaattcggg gtttgtttta tcaagcgacc gtcgaacctt
    12541 tcaacaattt tcgaatccaa gctcgatttg ggctaaggtt atgccaaata tcctcactga
    12601 ttggcacagg ctcattcaca atcaggcttg ctaacaattc ccctagaagt ggtgcagtcg
    12661 tcaaacctcg agaagacagg ccgtttacca tatacagatt ggtaaaaatt tcagcatttt
    12721 ctaccgcttg tttccgtctc aactggttgt ataaattggc atattggctt ttttgctgct
    12781 caaaatgatg cactgcccca accatcggta cacgatcacg aaacgccgca cgaataccga
    12841 ctttggcttt attttcagat aaatcaattc cttgcgtcca ctcacaaacg gttacatttt
    12901 gctgaagttt tgcaacattc tcccaatgct ccgataaact aaactcggtt tctgcattat
    12961 cccgaatatg gcttgctcca atgcaatgcg tgccataggc tgacattggc gttaaataac
    13021 catcataaca aagcacacac ttcagttttt gtaattcgct tgtagtcgga atttggctca
    13081 cttgccctcg taccggatac aacggaatgc cgttcgcttg gctaaattca ttcaacgtat
    13141 gtccatttgc cagcacaacc gcttgatgtc gaaccgtttc tcccctcgtc tgccattgcc
    13201 acattccatt agaaaaggtt aaatgctcaa ctttgtgatt cagtacaatc ttcaaccctt
    13261 tctgctccag ccaagtaaag ccatttctaa caagttgtac cggtgaaagc caaccagctt
    13321 gcggaataaa tgccccgctg tttggcacgc ttaaacctaa tttttcactt aattctgcct
    13381 gtgaacaaga ttttaataag gtgtcatcag ccgtctgctc caccattttt tccagttttt
    13441 tagccgtttt ctcgtcatag gcgtaaagca ctaccccgct taaattatgc tcaaactcaa
    13501 tgttggattt cagttgttgc aagcgttgca atgcataatc aaaactatgg atataaaact
    13561 gtacatttcg ctcgtcatca tcgcttaact gtgggtaaat cgccccttgt aaatttcctg
    13621 aagcatttat tgccaaggtt tcatctttgc aatacagcgt gacttgcttg cctttttcta
    13681 acagcgataa cgcaacgaag aaactcgcta ctccgccacc aataattgca atgtcatcag
    13741 cggtttcatt tttggcataa aaataggggt aattaggctc ggaaacttgg ctatctaacg
    13801 gtttctcgcc ccaaagcatt tctcgttttt tgccaaatcc tttgcgtttt ttcactgcaa
    13861 aacctgcggc ttccagccct cgtctgacca tacttgaagc ggtaaaagta gcaaaactgc
    13921 cgccattggc acttagtcga aacatttgca gatataactg ttcgttccac atttctgggt
    13981 ttttatcagg cgaaaagcca tctaaaaacc aagcgtcaat cgtgccgcta taaagatcgc
    14041 ctaattgggg taagttttca gcaatctcac cgaaccataa atctaaataa acctgctcaa
    14101 aatgataacg ttggcaacct atttggcgag gctgccaaca agcggttaaa ttttcagata
    14161 atgttgcaaa ttgcggatag cgttggtgaa tatcagctaa ttggctggcc gtcagaggaa
    14221 atttctcaaa tgagataaaa tagagtcgtt ttagtttact ttgcggaaat gctgcccgaa
    14281 actgttggaa tttgtcagca acggctaaaa aattcaagcc cgtgccaaag ccggtttccg
    14341 caataacaaa agcctctttc gggtgagatt gccacttttc ccacagttgg ttaccttctt
    14401 gaaacacata gtaactttcc gccaaaccat cttgggtaga aaaataaata tcatcaaatt
    14461 gctccgacac cggtgtatct gcatcattaa atgtaagtgt cgcaaaagtc agtttaccca
    14521 tcatatcccc ttaaaaacaa aacacggaaa accccaagat tttccgtgtt tcaaccagtt
    14581 caatagtacc aatgttagaa aatgttgctg gcaaaaataa cgatcatacc aagtgcgcct
    14641 acaaccgtta gcattgaata accaaaagta cccattttac aatcctcatt gttaattatg
    14701 attaaaacgg ctttattcta gcataatttc catcgctttg aatgcctatg cttgaataat
    14761 tttgccaaaa caagcggtca atttttgtaa atcttttacg cctttggcac tttccacgcc
    14821 agaatttaaa tctaacccga cacagccttg agcaagggct tgctcaatat tctcagtgcc
    14881 aataccacct gccaataacg ctttttgttt taaggtttct ggaatcaacg cccaattaaa
    14941 ggttttgcct gtgccgcctt gctgattgcc tactttgctg tctaatacat aacgagcaat
    15001 ctgtggatta tcgttaaatt ctagattttc aacctcaaca tcaaccgata aggctttcca
    15061 gatttgagtt ttactaccta atttctctgc cagttctgca atgtattgct catcttccga
    15121 gccgtgcaat tgaactgcaa ataactcaag ctgttttgcc attttttcca caaattcgac
    15181 cgcttgattt tggaacaccc ccacataacg caacggtgca ttcaccacca attcttgggc
    15241 ttggcgaagt gaaagctggc gaggcgagcc ttcggcaaaa attaaaccgc catataatgc
    15301 cccttgctca taaaccgctt tcacatcttg cacacgggtt aagccacaca ctttgttttc
    15361 accaaaaatc accgcccgca ccgcattatt gagatcggca cttcccatta aactgctgcc
    15421 gattaaaaaa gcatgaacat acggcttaat gtttttcact tgctggtgat cgttaatacc
    15481 cgattctgaa atcaaacaga catcagccga aatttgggct tggtattttt ccaccaaacg
    15541 cacaatgcgg ttcatatcaa tcgtgagcgt gtgcaaatca cggttattaa cgccaattac
    15601 cttcgcccct aaggctaagg cacgttcaaa ttccgcctcc gtgctggttt cagtcaaaac
    15661 gcccattcct aactcgtgag ccagtgtggc aagttcacgg taggtttcat catttaccac
    15721 cgataacatc aataaaatcg catcagcatt ataaaaacgg gcaaggtaaa cctggtatgc
    15781 cgaaatcata aaatctttac acaaaatcgg ctgtgtggtt tggcgtttga cttgatcgat
    15841 ataacggaaa tcgccttgaa aatattgctc atcggttaaa accgaaatcg ccgcagcgta
    15901 atttttatac acttgggcaa tactgtctaa atcgaactca gcacgaatta acccttttga
    15961 cggtgaagct tttttacatt ctaaaatata agctggttct tggtgtgtgc ctttggcaag
    16021 agccgcatag aaatcatggt cgctttttac aattttgtgc tgaaattcac ttaacggaaa
    16081 ggcttgctct ttttgtgcca cccaaatggc tttatcttgt acgatctttt gtaaaatcgt
    16141 cggttggttt ggttgagcgt tatcttgaac gatgttttgc atataaattc ctagagcgaa
    16201 atcggtttga atggtaaaat ctgcccttat ttttacccga aaaagtctca tttcacaagc
    16261 aaaactaaga tgaaatcaca agttatacag catttatctt atttagaaat cgccctgaga
    16321 gttaatcaac tttgggaggc gacaccacca agccctgaag ccttagccaa tgagcaacct
    16381 ttctgcgtgg aaaccttaac cccaacccaa tggctacaat ggatttttat tcccagaatg
    16441 cgggccattt tagatgcaaa tgccgattta cctcgcaatt ttgcaatcac cccttattta
    16501 gaagaggcat tgaaaaacga aggttattta caagcgattt gtgaaccgct attaggtttg
    16561 gaaaagttgt tgaaagatta acttctctct cagttctttt attaatctga caaattcgtt
    16621 gatagtgcca gcgtccacgc tggtactaat tttaacggtt aattaatcaa aatttggcac
    16681 aagcgaggac gcttgcgcca tcatagagat ttgataaatt atacttaatg tattatgacc
    16741 ttagatattt tataccaaga caactcgctt atcgccatca acaaacctgc cggagtgttg
    16801 gtgcatcgtt cttggttaga taaacacgaa acggtgtttg caatgcaaac cctgagggat
    16861 caaatcgggc aacacgtttt tccgattcac cgccttgacc gcccaacctc aggtgtattg
    16921 ctgtttgctc tcaacagcga aaccgcccga ataatgagcg agcagtttga acaacaccta
    16981 gtacaaaaaa cttatttggc ggtggtgcgt ggctatttac aaggttcggc acaaattgat
    17041 tatccgctca aagtgatttt agataaaatt gcggataaat tttctcagga aaaagaggct
    17101 caagcggcga ttactgatta taaaggctta gcaacggttg aaatgcctta ccctgtcggc
    17161 aagcaccaaa cctctcgtta tagtttggtt gaacttagcc ctaaaaccgg gcggaaacat
    17221 cagcttcgcc gccacctgaa acacctgttc cacccgatta tgggagacag caaatatggc
    17281 gacttacacc aaaaccgtgc tttagttgaa aaatcaggcg taagtcgttt aatgttacac
    17341 gcccataaat tgcaatttca gcacccgaaa aatttgcaaa aaatagagat aattgcatcg
    17401 cttgatgagc aatggcaacg cctctttgca ttttttgact ggaactttac tcaatattat
    17461 tagacaaaaa agaggattga atggataatt cacttttatc attgacacac gagcaacaac
    17521 aagccgcagt agaagaaatt caaaaactga tggcacaagg catcagtgcc ggagaggcaa
    17581 ttcaaattat tgctaatcaa ttacgtgaca aatatcaaat attggagaaa tcaaacaatg
    17641 aataaattat ggacagtgct tgccctaaca ataacttccc actatagctt tgctgccgct
    17701 gaaaaaatag tcactaaaga tttaccggca gaaggaaaac ctgcagtcgt gctacaggtt
    17761 tttgatgaaa gtgaaaaaat tgcaaaacct attaagggaa ataagctctt tattagcaaa
    17821 aaacaaacaa ggctttgctg gcacagtatt aatcttcctg tacaaaataa agttactatt
    17881 gcagaagctt tctatgcccc tgctgcaatg aaattagctt ctcccggctc taaaattaat
    17941 tcttctgcag acaagaaaaa tcatactatc attactgaaa taaaccttgc aaataatcaa
    18001 gtattaaatc gttgctgggg ttttgataaa actgaccctg tcggtaaata caaaatggaa
    18061 attcaaatta acgatcacat tttcaaaggt ttagaatttg aaattgtaaa ataacctaaa
    18121 agccacagag taacaccctg tggctttttg ctaaatcatt gtttttaaat tggttaaatt
    18181 gtgcagcaaa taatctttac tcttcacaaa ggctctcgcc tgttgccagt tgctaaattg
    18241 ttgctgagtg cattgattaa aacgtaccag cacttccaaa ctttcattgc cgacacaaat
    18301 aaaatcagct aataattggg ttaatttttc actgttttca ccatccattg cagttgcaat
    18361 tttctgctcc catttaaccg cttgtccgag caaataaccc cgctgggctt tgcttaaatt
    18421 actcaaccac atcccatccg cttttggcat tgcctcaacc aaagtaaata agtcgcctag
    18481 atttccttgc ttgatttcaa actccaattc acaaatcggc tcttcaccaa aatgattttt
    18541 aatgatgccc tgatctaacg ctacctcaat gtcagacatc cccacttgca ccaaccaagt
    18601 tttacgagtg aaatcggtgc tgaatatcgc ttgcaagctt tctgcaatct gctctgaatt
    18661 ttcaaacggc aactggtatt tttccactaa tgtttgaaaa tccggctggc tattgggtaa
    18721 agctaaatta tattccggac gaatatgcaa accgcctaca atttcccctt gtgtttttaa
    18781 cgtgatttca tattgttgat tcacagaacg cacccgcagc cccatttttt gcgtggcaaa
    18841 aaattgctcg ggcgtatcat aataggtgtt gcctaacaat tccgtttgat gagcaagcac
    18901 agggaatggc gaggtttgat tcaagtagtt ttccactaac gccacattct gctgttccaa
    18961 cattattttc agttcgattt ctgcttgttt ttggttttcc atttcacacg cccctgcaac
    19021 gttgtataat caagcggtta ttttatcaat aaattttgca aaatgttttc aaaaattcac
    19081 cataaactga ataactttgt ccagcgtgga ctcgacaacc atttacgcct cgccgttacc
    19141 ggcttaagcc gtagtggcaa aacggcattt attaccagct tggttgatca gcttttgcat
    19201 atcaacaaag gcggtaattc gcatttaaac ctgtttgcgc ctgcacgcca cgggcaaatt
    19261 ctatcggtaa aacgcgttga gcaaggggat ttaactattc cccgctttga atatgataaa
    19321 aaccgccaaa gccttgaaaa tcaaccacct caatggcctg aatccaccac tggcattagc
    19381 gaaattcgcc ttgcgattcg ctaccagcgg caacattctc tgctacgtca tattaaagaa
    19441 accggcacgc tttatttgga tattttcgat tacccgggcg aatggctgtt agatttaccc
    19501 ctgctctccc tttcctataa agaatggtcg caaacgcaac aaaccataca taaagatgag
    19561 cgtgcagagc ttgccacacc ttggttacaa gcggttaaaa aactcgatct ttttgcaaaa
    19621 accgatgaaa atttactggc agaattcagc caacaataca ccgattattt gctcgcctgc
    19681 aaagctcaag gaatgcaata tattcagccg gggcgatttg tgttgccaac tgaaaatgcc
    19741 aaaggtgcac cggtattcca attttttccg ttattagatt taagcgaagc tgagtgggaa
    19801 aagttggaac aaagcgacag aaacagtatt ttccataccc tcaaaaaacg ctatgagcat
    19861 taccagaaaa aaatcgtcaa accgttttat gaagattact tctctcaatt tgaccgccaa
    19921 gtgattttgg cagactgcct cacaccgtta aattatggct ataacgaatt tatggaaatg
    19981 aaattagggt tacagcaact gtttaaacat ttccattatg gcaatcgttc gctatttcac
    20041 cgtctgtttt cgtccaacat tgataaattg ctatttatcg caacaaaggc agatcacatt
    20101 actagcgacc aagtgccgaa tttggaaagc ctaatgcgac aattagtgca agaaggggga
    20161 agacacgcag aatttgatgg tatcgaaacc ggctatttag ccatttcctc gattcaagca
    20221 accgatgcgg tgaaagtgac tcagcaagat aaaatcatta aagcgataag agggatccgt
    20281 tccagcgata gaaaacaagt gacactctac cctggcagcg tgccaaatcg catacccgat
    20341 aaaaattact ggcaatttaa ccgttttgaa ttcgaccaat tcgagcccaa gcaacttgat
    20401 tatagccaag cgattccaca cttcagaatg gattcggtgc tacaattttt attggcggat
    20461 atgtttgagt aaaatcttgt tgcatatttc cattaaaggt ataaaatttt atacattatg
    20521 aatactgtaa tttcccaacg aaaaccaatt cactgggttg gatcaacctt agatgatatt
    20581 cgtgaatttc cggataacat taaaagagaa ctcggttttg atcttgattt agttcagcaa
    20641 ggcttattac cgagggattt taaagcgatg gcaaatttgg gttcaggcgt aatggaaatt
    20701 cgagtaaaag atccctcagg ggcttttcgc ttagtgtatt tagctaaatt taaagatgca
    20761 atttactgtt tacacgcttt ccaaaagaaa acgcaaaaaa cagcccctaa agacattgct
    20821 gtcattaaag cacgctatgc tgccgtaaag gagttagaaa atgaataata ttgatactaa
    20881 aattcgccat actacagaag ctcagggtaa tgtttttgaa gatcttggtt tccctattga
    20941 tgaagctcaa aaattgaaat ctgccagtca gcaactgatt gaaactaaac tgatgctaat
    21001 gaatgaaatg agcaactgga ttgataaaaa taatctcaaa caatcagaag cggcttctat
    21061 tctaggtgtt agccgccctc gaatttctga tatggtcaat ggcaagttag aaaagtttac
    21121 cctagatacc ttagtcactt ttgttgcaaa aaccggcaaa acaatccaat tgcaattatc
    21181 ctgatatttg caaaatttta ctcaaattca accgcttgtt gcttttatca ttcactaaca
    21241 aggttatttc aagtaaaatt gtgactaatt ttttattaat aaacacaaat tatggcaaaa
    21301 aaacctaaag ttgcatcaaa tacgattgcg ttaaataaac gtgccagaca cgaatatttc
    21361 attgaagatg aaattgaagc cggcttagag ctacagggct gggaagtcaa agcattgcgt
    21421 gccggaaaag gcaatatcag cgatagctat gtcatctttc gccacggcga agccttctta
    21481 ttcggggcaa tgattacgcc tttgaatatg gcatcaaccc atattgtcag cgacccgacc
    21541 cgcacccgca aacttctact gaataaacgt gagttagact cactgttcgg taaagttaat
    21601 cgtgaaggtt ataccattgt tgccacctca atgtattgga aaaatgcgtg ggtgaaagta
    21661 aaaatcggct tagccaaagg gaaaaaactg cacgataaac gtgaagatat taaagaccgt
    21721 gaatggcaag tggctaaaca gcgtattatg aaaaacgcca atcgtggcta aatgaataat
    21781 taccctctcc ccttgtggga gagggacaga aaaattgcgt tcagcaattt ttcagggaaa
    21841 gggggatttg taaaagttca aggaaaaaca acgcttgccc tctctctgaa aaatatttct
    21901 gaacgaaata tttttcggtc tctctcccat aaagggagag agtgaaacac aaatttaaac
    21961 taaggaacat acaatgatcg accaaaacct acttcgtacc aatttagaag acgttgctac
    22021 agctcttaaa atcaaacgta attttatttt agatgtagag cgagtaaaaa cccttgaaga
    22081 acagcgtaaa acgctacaag ttaaaaccga aaccttacaa gcagaacgca acactcgctc
    22141 caaaaacatc ggtgcggcaa aagctcgtgg cgaagatatt tcagctttat tagccgaagt
    22201 agattcaatg ggcaacgaat tagacacggc aaaagtagaa ttggataaag tacaggcaga
    22261 aatccgtgag ttgttgcttt ctgtgccaaa cttaccggca gatgaagtac cactcggtaa
    22321 agacgatagc gaaaacttag aagtattacg ttggggcgaa ccacgtcaat tcgactttga
    22381 agttcaagat cacgttaccc ttggcgaaaa cttaaacggt ttagattttg ctgccggtgt
    22441 aaaattaacc gccagccgtt ttgtggtaat gaaaggtaaa cttgcccgct tacaccgtgc
    22501 attatctcaa tttatgttgg atttacacac tgaacaacac ggctatgtgg aaaccaatgt
    22561 gccattttta gtcaatcacg acactctata cggcacaggt cagctaccaa aatttggcga
    22621 agatttattc catactcaac cattaacagg gcaagatcca aacgaggtgc aacgcccatt
    22681 cggtttgatt ccaaccgctg aagtgccagt aacgaactta gtgcgagatg aaatcattga
    22741 tgaagacagc ttaccactgc gttataccgc ccacacccct tgtttccgtg cagaagccgg
    22801 ttcttatggg cgtgatactc gtggtttaat tcgtatgcac caatttgaaa aagtggaaat
    22861 ggtgcaaatt gtcgcccctg aaaaatcaat ggaagcctta gaagaattaa ccggtcacgc
    22921 agaaaaagtc ttacagcttt taggcttgcc ataccgcaaa gtgctactct gcactggtga
    22981 tatgggcttt ggtgcagcaa aaacctacga cttagaagtt tggctaccgg cacaaaacac
    23041 ttaccgtgag atttcttctt gctcaaatat gtgggatttc caagcccgcc gaatgtctgc
    23101 acgttgtaaa gctaaaggcg acaagaaaac tcgcttggtt cacaccttaa acggctcagg
    23161 tttagcggtt ggtcgcacac tggtcgctgt gttagaaaac taccaaaatg ccgatggctc
    23221 tattactgtg cctgaggtgt taagacctta tatgggtggt ttggaagtaa ttaccgctta
    23281 agattaataa atgaaaccaa tctcagaaat ggggttggtt tttttaataa atcttatccc
    23341 taatcttttt caggataatt cataaaaatt tcagtaacta ctcggcggtt ttgcactgta
    23401 tctaaacgaa taggattttt cattctccct aaacgaaatg caataatggt gacttttttc
    23461 ggttcatcta ttttatctaa ttgatagaat accattattt cccgttgata aaccgctcgc
    23521 tttctgaaac gtaaatcctt gcgaaatgcg acacctgctt ttggataaaa ctgtaaacca
    23581 ttgatgatat tcgtttgagt atgctccgaa aataatccat agaaaataaa ctctaataga
    23641 ttatttttaa aatcaacact aaccatctaa ctgctcccaa gcagttaaat ctcctcgtga
    23701 taaacgttca gcaatatcag cataaaattg tttcggtact tcccttttta cttggtattg
    23761 agctgattgt tcttctaata aatgttcttc tatcatcttg atttcttctg ttgtccactc
    23821 ttcatcttct ttatgtacaa caaaatcggt atctacaatc acaatagaca tacctagctc
    23881 cttcaagtac tggttatata ttctgtattt tgcctgttct aaattcagct gtctagcttt
    23941 tctgttactc acaatttgca aatttttcat caaattccac cgcttgccat tcaaaaaaag
    24001 gcgtttttgc ggtatcctat cggcaatttt ttcagttaaa tttaaaggtt aaattttatg
    24061 caacagcagt ataacccaag tgagattgaa cctaaggttc agcgttattg ggcagaaaat
    24121 aaagttttta aagcagttaa agacgttacc aaagagaaat actattgcct ttcaatgttg
    24181 ccttatccgt caggtaagct acatatgggg cacgttcgta actacaccat tggtgatgta
    24241 gtgtctcgtt atcagcgtat gatcggtaaa aatgtattac agccgatggg ttgggacgct
    24301 ttcggtttac cggcggaagg tgctgcggtg aaaaataata ccgcaccggc aaaatggact
    24361 tacgaaaaca tcgaatatat gaaaggtcag cttaaaatgt tgggcttctc ttatgactgg
    24421 gatcgtgaag tcaccacttg ccgtccggaa tactacaaat gggagcaatg gttcttcaca
    24481 gaactttata aaaaaggctt agtgtataaa aaaacctcaa ccgtaaactg gtgtccgaat
    24541 gatgaaactg tgcttgccaa cgagcaagtt cacgaaggtt gttgctggcg ttgtgacacc
    24601 ccagtggaac agcgtgaaat tccacaatgg tttatcaaaa tcactgacta tgctgaagaa
    24661 ttactcaatc agttagataa tctcccacag tggccggatc aagtaaaaac catgcaacgc
    24721 aactggatcg gtcgttccga aggggtagaa atcaccttta aaattgccgg ttcaaacgta
    24781 gaattaccgg tttacactac ccgtccggat accttcttcg gcgtaagcta tgtggcgatt
    24841 gctgcggctc acccattagc agaattagct gcacaaaata acccggcatt ggctgaattt
    24901 atccgtgaag cgaaaaacac caaagtggca gaagcagaat tagccaccat ggagaaaaaa
    24961 ggaatggcaa ccggcttgtt cgccattcac ccgttaacag gaaaagaagt accggtttgg
    25021 gttgctaact ttgtgttaat gcactacggt acaggtgcgg taatggcggt tccggctcac
    25081 gatgagcgtg actttgaatt tgcacaaaaa tacgatttgc aaattaatca ggtaattcaa
    25141 ccgcttgatg gctcagagtg ggatttcagc aaagccgctt acaccgaaca cggtaaatta
    25201 atcaactctg ccgagtttga cggcttggaa tttgatgtgg ctttcactgc gattgccgat
    25261 aaattagagg caatgggtgt aggtaaacgc caagtgaatt tccgtctgcg tgactggggt
    25321 gtttctcgtc aacgttattg gggtgcacca atcccaatga tgaccacaga agagggcgaa
    25381 gtggttaccg ttccattaca agatttaccg gtgattctcc ctgaagatgt ggtaatgaac
    25441 ggcgtgcaaa gcccaatcaa agccgaccca gagtgggcga aaaccactta caacggcaaa
    25501 ccggcattgc gtgaaaccga taccttcgat acctttatgg agtcctcttg gtattatgca
    25561 cgttatactt gcccacaata tcacgaagga atgttagatt cagacgaggc aaactactgg
    25621 ctaccggtcg atcaatatat cggcggtatt gaacacgcca caatgcactt gctctacttc
    25681 cgtttcttcc ataaattgtt gcgtgatgcg ggtattttaa attcggacga accggcgact
    25741 aaactgttat gccaaggtat ggtgcttgcc gatgcgttct actacaccag cccaactaac
    25801 gagcgtattt gggtcagccc gacacaagtc accttagaac gtgatgagaa aggtcgtatc
    25861 atcaaagcaa ccgatccaca agggcgtgag ctggttcaca gcggtatgac taaaatgtcg
    25921 aaatcaaaga acaacggtat cgatccgcaa gaaatggtgg aaaaatatgg tgctgacaca
    25981 gttcgcttgt ttatgatgtt tgcctcccct gcggaaatga cattagaatg gcaagaatcc
    26041 ggcgttgaag gggcaaaacg tttcttaggt cgtgtttgga atttagttta tagctatgcc
    26101 caaaatccgg caacgactgc attagatgtg aatgccttag acaaggatca aaaagaactt
    26161 cgccgtgatg tacacaaaac gattgctaag gtgagtgatg atattggtcg tcgtcaaacc
    26221 ttcaatacag cgattgctgc ggtgatggaa ttgatgaaca agctcaccaa agccccactt
    26281 gaaaccgaac aagataaagc cgtaatggca gaagctttaa gtgcggtggt gcgtatgctt
    26341 tacccaatca cgccacatat ctgctttgag ttatggcaag tgttaggtaa tgaacaaacc
    26401 atcgattttg ccccttgggt acaagccgat gaatccgcaa tggtggaaga tgaaaaatta
    26461 gtggtagtac aagtcaacgg aaaagtgcgt ggcaaaatca ctgtttcagc tactgctacg
    26521 gaagatgaag ttaaagctat cgctaaagcc gatccaaacg ttgctaaatt cttagatggt
    26581 cagcagattg taaaagaaat ctacgttcca tttaaaatgt tgagctttgc ggttaaggcg
    26641 taacaaacac actctccctt atatgggaga gagaccgata aatatttcgt gcagaaatat
    26701 ttatcagaga gagggtacaa gcggttattt tcttggaaaa ttttgcaaaa aatagccccc
    26761 tctccctgct ttttcttcta aacgaagaaa aacctgtccc tctcccacaa ggggagaggg
    26821 gaataaacaa ggaaatctca ttatgattaa aaaactctct ctcatcgctg tttttgctct
    26881 taccgcctgc ggttggcact ttaaaaacaa tgaggtatta cctgaatcat tcagaacatt
    26941 aacctttgaa agtgcagatc aacacagcga aatgtctcga attttacgca atcagttaca
    27001 actaagcgat gtaaaattag tgcctagcac ggctaatgtg gcaaaattac gtttggtttc
    27061 aacctcaaca gacagcaaag tggtttctgt gtttaaacaa gctcgtgaag cagaaaaaat
    27121 cttaacgcta aacgttgatg cgattgccga tattccaaat aaagggcaat accctttaga
    27181 agtttcggtt catcgtactt tctttgatga ctctcgtgca gctttggcaa agtctgctga
    27241 aaaagagatg attatgaaag atatgtatga acaagcctct cgccaattaa ttatcaaaat
    27301 ggcgggctta aaaaaagaac tctcaaaata atcattgtag gggcgaaata tgtttcgccc
    27361 attgtttatc tgtatgcaaa aaatctttcc ggaaacactt caattttcat taaacaaagg
    27421 actacagccc ttttatctat taacagggaa tgatctgcta ttggtgaatg aaaccaaaga
    27481 tgccattatt cacaccgccc gtctcaacgg ttttgatgaa aaaaaagaag ttgaaatcaa
    27541 aaacgatact aactgggaag aacttttcga agcagctcaa tcaatgggat tgttttttgc
    27601 acgccaaatt atggtgctga attttccgga ttcccccaca gcgacacagt ttaaaaaact
    27661 ggctgagctt tgtgccttta ttcatagtga tttgctcttt atcctgcact tacctaaatt
    27721 ttccaaaaca atggaaaaac aggcttggtt cacgcaaatt gagctaaatc tcgtgcaaat
    27781 taactgccaa acaccggaac ttagcaaact tccacaatgg gtcagtcatc gagcaaaagc
    27841 gatgaatttg caaattgaaa atgaagcaaa ccaactcctt tgttatagct acgaaggcaa
    27901 tctactggcg cttaaacagg cattacaact gctacaactc aatgcaactg atggaaaaat
    27961 cacgctgaca aaagcccaag aagtggttga acactccgcc caatttactc catttcaatg
    28021 gattgatgca ttatttgaag gaaaaatcca aagagctcaa cgcattttgt accatctgca
    28081 aaatgaagaa gtacaagcgg tcgttttatt aagaattgtg caaaaagagt tgatgatttt
    28141 actcgaaatc actcgcaacc caaccgcagt cgtttccagc gaacaatcac tttttatcgg
    28201 taatttacga acagaatttg accgcttgaa aatttggcaa aaccgcagac ctttttacaa
    28261 ccagatcact caacgactga cttaccaaaa actttacgcc ctaatccaac aactggctga
    28321 attagagcgt aaggtgaaac aagaatttag cgatgatatt tggcaagaat tagagcgttt
    28381 ttccgctcaa tttacctaac taatactttt ctaaatcttc gctaaacaac tccgcttctt
    28441 cactggcgat agagtgcttt tctgccgccc aatcgcctaa atcaataatt tgacaacgct
    28501 cgctacaaaa cgggcggtat ttactttctt tactccacac cacttctttt tcacaagtcg
    28561 ggcagtttac aatagtttct ggcattcttt ttcctttttt gctaatgcta agtatttata
    28621 atgtaaggct tgaacctgct ctttaattaa aagcaagccc tgttcaaatg gcaagttatt
    28681 ttcaatcaca tcatcagcat aacttaatct ttcttcacgg ctgacttggg ctgccataat
    28741 atttttaatg gtttcaattt tactgttatc acgtttggtt gctctttcca gttggatttc
    28801 aggagaaaca tcaacaacca atatacgatc acaatattcg gtgagcttat tttcaattaa
    28861 aagcggcacg acccaaatca cataacaagc ggtcgattct tgcaattttt ttaccatttc
    28921 ttctcgaatg gcggggtgaa gtaaattatt gagccacgtt ttttcctctt cattgttaaa
    28981 cacaatttgg cgaagtttag tgcgttctaa tgctttttca gcggttaaga tctcttcacc
    29041 aaaatgctct acaatcttgg ctaaaagcgg tgtacccggt tcaacaactt ggcgagccac
    29101 cacatcagca tcaattacct ctaccccaag agctgaaaat aaatcagaca ctgtggtttt
    29161 gccactgcct attccaccgg ttaagcctac aacgtaagtc ataaaaattg attcctcaaa
    29221 ataaaaacaa accaattcta gtaattaatc cctctgcttt caagcattag caccagcaac
    29281 atcgctgtgc taataaacgg aataaacgcc aattttgcta atttctgctt tttcattagc
    29341 caataagcca agtaataacc aatccctagc aggctggcgg ttaatatcaa taagagcatt
    29401 ttttcgagcc gaaagagtgg ggataacgca accaataaca acatatcgcc cgagccaaat
    29461 gcctcctttt taaacataag ctgtgaaacc aagccaatca acaggcaaaa agcaagggta
    29521 aaggtaaaag tttggtgttg gaaatcggct aaactctccc ataacaccaa caaaaatata
    29581 agcacgaggt agcgaatatc cgttaggtaa tagcacatat ccagcagtga taaaaaacag
    29641 agcagaataa aaatcgtgcc gataataggg ctttccgagc agagcataat caatggaaaa
    29701 aggagaaaaa atagatttgc cgatcggggt tgtttcggtt gtagcaccga ttgagccaaa
    29761 aactgttgct cggttaagcc aatattcact aatgaagttg tttcttggta aatttggcga
    29821 ttgactcgaa tggcaaatcg agggatctcc gctttgagcc aaaccgcaat gaagagggaa
    29881 aacagtatta acatcgctac tccaccaaag cccccatatc aaaaatcggt aaatacagcc
    29941 caacaataat agtgccgaca atcacgccca ttatcaacat tagcatcggt tctaatagct
    30001 gagacaaaat atcaatttgg taatctaatt tttgttggta catctcacta atatgctcac
    30061 acattttcgc cacattgcca cttttctccc caacctcaag catttgtgcc atctcagcac
    30121 caaacacatt cgggttgatc ccttgtgaaa atttgtagcc ttgttgtaac aaattaagca
    30181 tagtgccaat ttcttgctgt aaaatcgggt catcattttt ctcggataaa aacgacctca
    30241 aggcaacatc aagcggaata tgagcattca gcattaaggc aatattttgg ctgaaaaaga
    30301 taatgcgaga ttgctcaata attcggctaa acagtggcat tttagcccat agagccattt
    30361 tccactttgc gactaacctg ctcttttttg caaaaaacag gtaaaaaatg accgctagta
    30421 gtaacgtgcc aagtaatact gtgccttgct caatcaagca ttcggaaagg gtaaataaaa
    30481 tatcggtaat gaggggtaaa gttttgtctt ttgagccata aagttgggca aactgcggta
    30541 caatcaaaac caatagcccg atagataaag cgagcgaaat cagcaacaca atcaccgggt
    30601 aaaacaggat tttcttcact tttttcgcca atttttccaa tttcgctcgg ctttcagcta
    30661 aattagtcag cataatgccc agttttccgc tttgttcgcc cattttgatt agctgaattt
    30721 cctgattggt cacaaattca cccaatttct ccaacgattg tgaaaaagca tagccacttt
    30781 caatatgctg aatcagctca ttaagccata gataaaggcg gatatttcgg cagttttgca
    30841 acaacataga taatgcctgt tttaacggca tggcagaatt aaccaacaat gcaagctgtg
    30901 atagcaagct attcacctct tcttttttcg ggtttgctga aagcacaaaa tttcggctaa
    30961 tccgaatatg ctgataacct tgtgccagca aaaggttttc caacatgtct cgattcgttg
    31021 ccaaccgctt gccccgctgt ttttgcccaa atcggctctg ggcttgccaa tgaaattcat
    31081 agattttcgt cattgcctaa gcaatttctc tttttgtggt tcaaaatgaa tatctgaggc
    31141 gttgtgttgc aaacagaact taaataaatt ctctaaaatt tggataatcg gatcgtttct
    31201 atccacttgt tccgaatgag ccaatgaata cgccgtattc tcctcataaa tctcactctg
    31261 ccgactactc tgctcaggcg aaagggcatt gagtaaaaac ttcaattcat cggaggaaat
    31321 caaaacaggc tcaatctgtt tgtggtagat aaacgcaaaa atctcacagg cattgagatt
    31381 ttgcatatca tcaatcccta gccaaagctg ttcttctgtt tcttgcagcg gaatggctaa
    31441 atatcgcaat aaaatctgct tttcattgca atttttctgc cactgctctg ctgaaatctc
    31501 gatgaacctt tggctgttta aatccgttac cgaatactcc attttgattt cctcacctca
    31561 aattagccgt ttggtgtaac agttgtgcta cagaaattag ctggaaatag gctcgtatca
    31621 ttgccactac aacttgccgt ccaactaatc gtattatttg agaacgacgg tgttaaggta
    31681 tagccataac cattcagaga gcctttgccg gttaccgtaa tcacgccttt tgttaccgca
    31741 attgagttta ggtatttagt ggtattacta ctggttttat tcgcctgaat cccattcgtg
    31801 ccaccggagc agttatccaa cccgccactg ttgtaaatac agacttcaac gtctgattta
    31861 tacgaagcag atgcttgtaa taactccgat aacgccgcct tttgggtgta tgagttatag
    31921 gaagggatcg caatagtggc aagaatggca ataatcgcaa tcacgatcat caactcaatg
    31981 agggtaaatg cccgatgcag ttggcgaatg ccaagcggtc taaaatgtac aattttttgc
    32041 atataaattc cttataaatt gcaccaacaa attgtggcga atcaagtgtg caaatttttt
    32101 gcctgctttg cgaccggcaa attttaattt ttcgacgcag atcgaaaaaa taaaattttt
    32161 atctgtttat ccatacagtt ttttgttaga atttgccaaa tttactcaat taggaatcaa
    32221 gaatggcaaa agcacctaaa accgcttatg tttgtaacga ttgcggggct gaatattccc
    32281 gttggatggg gcaatgtaag gaatgtaagg cttggaatac catcagtgaa gtgcggttga
    32341 tttccagcaa agaagcggcg aaatctgacc gctttagcgg ctatgccggt gaaacgtcag
    32401 gcaaagtaca aacgctgtct gacatcagct tgcaggaagt gcctcgcttt agcagtggct
    32461 ttaacgagct ggatcgtgta ctcggtggcg gcgtagtgcc ggggtcagca attctgatcg
    32521 gcggtcaccc gggggcgggg aaaagtacgt tattactgca agtaatgtgc ggtttagccc
    32581 aacatctgcc aacgctgtat gtcaccggtg aagagtcgtt acaacaagtt gcaatgcggg
    32641 caaatcactt aggcttacca acggcaaatc tgaaaatgtt gtctgaaacc tctgtcgagc
    32701 atatttgcaa tattgcagat caagaaaagc ccaaaattat ggtgattgac tcgattcagg
    32761 tgatgcactt gtcggatatt cagtcctcac ccggcagtgt ttcacaagtg cgtgaatgtg
    32821 catctttcct tacccgctac gccaaaaccc gccaagtggc gattattatg gttgggcacg
    32881 tcactaaaga tggcacgctt gccggcccga aagtgttgga acacgccatc gatgcttcct
    32941 tgctgttaga aggcgaatcc gattcccgtt tccgtacttt gcgtagccag aaaaatcgtt
    33001 ttggtgccgt caatgaattg ggcgtatttg cgatgaccga acaagggcta aaagaggtta
    33061 aaaacccatc tgcgattttt ttaagccgta gcgaagagca aacgccgggc agttcggtga
    33121 tggtgttatg ggaaggaaca cgcccattgt tggttgaaat tcaagcctta gtcgatcact
    33181 caatgctggc aaaccctcgc cgtgtggcgg ttggcttaga tcataaccgt ttatcgctat
    33241 tacttgcggt gctacaccga cacggtggct tgcagatgtc ggatcaagat gtgtttgtaa
    33301 atgtggtcgg tggggtgaaa gtcacggaaa ccagtgccga tttagcctta attttggcgt
    33361 taatttccag cttccgtaac cgtccattgc cgcaagattt ggtgattttt ggggaagtcg
    33421 gcttagcagg ggaaatccgc ccggttacca gtgggcaaga gcggatcagc gaagccgcaa
    33481 aacacggctt taaacgagcg ataattcctc acggcaatgt cccgaaaaag gcaattaaag
    33541 atatgcaggt gttcaccgtg aaaaaactca gtgatgcgtt agatattctg tataatctct
    33601 aaaatcaaga accgcttgta agtctgtctt acaagcggtc tgttttaggt aaaaatttac
    33661 gaatgtgcgg cagttggtct attgcctttg gaaatatagt ataaattaat acacaccacc
    33721 aacgcattta agccggtgac cggaatcgag ccaatcagca aaccataaac aacgaaacag
    33781 gcacaaccca gcgaattcac cacccgcagt ttaatcacat cttttaataa aaaagaacag
    33841 gcaactaaaa tcatcgccac atagccgagt atttcaacat agtccattta gaacctcctc
    33901 aaataaaata aactgccgat attttaaagg ctgttttttt atttgtatct agctctaaaa
    33961 atagcaaagg tttgcgttca catttttact tttaaccgct tagttattaa acagattttc
    34021 gttctaaaca cataaaacgg caacggaaag ggttattttc atcaatttct gaccactttt
    34081 cttctactaa cgcccaattt tgttcatcaa attcaaagaa cgtatcccct tcaatgtctg
    34141 cttgaatctc agttaaataa agtctttctg ctagtggtaa ggcttgctta aacagttccc
    34201 ccccaccaat aatcattatt tcagctgtat ttgcaaaatt tttcgctaat tcaaccgctt
    34261 gttcgagcga ttgtaccgca taagcccctt ccaccgtaaa acctgaacgg gaaagaataa
    34321 tattagggcg ttttggcaat aacctgccga tagattcata ggttttccgc cccataatca
    34381 ccggtttgcc gagcgtgttt tggcgaaacc aggctaaatc taccggcaaa tgccacggca
    34441 tttggttatc tttgccaatc accttgttga ttgttcgggc aacaataatg ctgattttca
    34501 ttttttctcc tgttatgcaa attggtttga ttttatatca aattaagcgt gattttacac
    34561 cctaaaagtg atacttttgg caacattatt tttagttaca agtgaacaga ttatgtcaaa
    34621 aactatcgtg ataaaatttg gcaccagcac tctcacacac ggcactaaaa gtttaagccg
    34681 tccttatatg ttagagctgg taaagctcaa ttacaccaac agcatcgtgt gattgtggtg
    34741 acatccggag ctgccgctgc cggtcgggac tatttaggac accctgaact ccctaaaacc
    34801 ttagcctcta agcaaatgtt agcagcggtc gggcaaagtc agctcattcg agtgtgggaa
    34861 aatttattcg atatttataa tatccatatc gggcagatgc tcttaacccg agccgatctg
    34921 gacgatcgtg aacgtttcct caatgcccgt gatacccttg atgccctgct tgcacaaaaa
    34981 atcattccgg tgattaatga aaacgatgcc gttgctaccg aagaatttaa agtaggcgat
    35041 aacgataatt tatcggcttt agttgcgatt ttggctcaag cagatcagct ctatttatta
    35101 accgaccaag aaggcttatt cagtgccgat cctcgctcaa accctgatgc taaattgctc
    35161 tcggtggtgg aaaaaatcac gcctgaaatc cgccaaattg ccggtggtag ttcaaccggt
    35221 ttaggcacgg gcggaatgag taccaaaatt actgctgctg atattgctac ccgctccggt
    35281 gtggaaacca tcatcgcctc cggttctcgc ccaaatgtgt tagttgattt agcaaacggg
    35341 caaaacatcg gcagtaaatt tttagtccaa tctgaccgct tggaaggcag aaaacgctgg
    35401 ttatttgctg ctccgcaagt gggtgaaatt gtggtagatt tcggggcgga acaagccttg
    35461 cttcaacata aatcattact gccggtggga gtttctgcca tcaaaggcga atttactcga
    35521 ggtgaagtaa tcaaaatttt cagccaagac ggcaaagcct tagcactcgg cattgcccgt
    35581 tatagcagtg aggcattgct gcgaattaaa ggtaagaaat caaaagaaat tgaatcgctc
    35641 cttggttatg aatttggttc tgttgcagtg catagtgacg aaatggtggt gtattaatgg
    35701 aattgggaat tgatgtttta gcaatcttgt ttgccgttgc gacccttgcc ggctttatcg
    35761 atgccatcgc tggcggcggt ggcttactca ctattcctgc attaatggca gcagggcttc
    35821 ctccggcaat ggcacttggc acgaataaat tacaggcgtg tggcggatca ctctcttcct
    35881 cactctattt tattcgcaaa aaagctgtta atttaaaaca aatttggttg ctaattctat
    35941 gcactttttt aggctctgcc gccggtacta tttttgtgca aatgatcaat gtcgatttct
    36001 taaaaacatt gttgcctttc ttaattctga ttatcggcat ctattttttg gtcagcccta
    36061 aaattggcga tgaagatcgt aaacaacgca ttagctttac cctatttgcc tttaccgccg
    36121 ctgtcgctct cggtttttat gatggaatgt tcggaccggc aaccggctct tttatgactc
    36181 tggcatttat tttgctgctc ggcttcaacc tctctaaagc cgtagcacac gccaaagtga
    36241 ttaattttac ctccaacttt gcctcattaa ttttctttac gcttggtggt gcggtaatgt
    36301 ggaaaatcgg cttaattatg atggtcgggc aatttattgg cggcacacta ggtgcgaaaa
    36361 tggtgatgac aaaaggcaaa aaattaatcc gtccgatgct agtgactatg tcttttatga
    36421 tggtcggcaa aatgctctac gagcaaggaa tttttaatta acaatgaata aagcaaaacg
    36481 tattgagatt ttaacccgtt tacgcaatga aaatccaaaa ccgacaactg aactcaatta
    36541 cagtaatccg tttgaattat taattgccgt gattctatcc gcccaagcga ccgataaagg
    36601 cgtaaataaa gccaccgata agctcttccc tgtcgccaac acgccacaag cgattttaga
    36661 tttaggcgta gatgggctaa aagagtacat caaaactatc ggcttattta acagcaaggc
    36721 agaaaatatt atcaagacct gccgagatct gatcgaaaaa cataacggcg aagtgcctga
    36781 agacagagat gccctcgaag cccttgccgg tgttggcaga aaaaccgcca atgtggtgat
    36841 gaataccgca ttcggacacc ctacgattgc agtcgatacc catattttca gggtgtcgaa
    36901 ccgcacgaac ttcgctcccg gcaaaaatgt agttcaggta gaagaaaaac tgctcaaagt
    36961 tgtgccggac gaatttaaag tcgatgttca ccattggttg attttacacg gacgctacac
    37021 ttgcattgcc cgtaagcctc gttgtggttc ttgtattatt gaagatttat gtgagtttaa
    37081 agagaagacg gaataacatt atctggtggt atttaatgaa taactcccct ctttagaaaa
    37141 gtaggggctg ggggagattt gatactattt caacatttcg tatgagcgta tagaaattct
    37201 gccaaatctt ccctatcccc tctttgctaa agaggggaac tagatcgagc gtaaaatata
    37261 acaagcggtc atttttttga gattttttgc aaatggatat ttatttagtc ggtggagcag
    37321 tgcgggatca acttcttggg ctgccagtga aagatagaga ttttttagtg gttggcgcaa
    37381 ctgccgaaca acttttagcc caaggatttc aacaagttgg ggcagatttt ccggtgtttt
    37441 tacaccctga aaccaaagaa gaatatgccc ttgctcgcca agaacgcaag aacggcaatg
    37501 gttacaacgg ctttatttgc gatttctccc caaacgtcac gcttgagcaa gatttgatcc
    37561 gccgagattt aaccattaac gccatcgccc aagatgaaaa cagcacactt tttgatcctt
    37621 atggcggtgt gcaagattta gaaaatcggc tactccgcca cgtttctcct gcattcagcg
    37681 aagatccgct acgggtgcta cgggttgccc gctttgccgc ccgttttcat tcatttggtt
    37741 ttaccattgc tcctgaaacc ctgaaattaa tgcgagagat ggtacaaagc ggtgaattga
    37801 aacatttaac cgctgaacgt gtgtggttgg aaacacagaa agcctttgaa accgataatc
    37861 cgcacgttta ttttgacatt ttacgcttaa ttggggcatt aaaagtgcta ttccctgaac
    37921 tggatcgtct gtttggcgta ccacaaccac ctcaacacca ccctgaaatt gactccggca
    37981 aacacactct aatggtgcta caacaggcaa aaagacttgc aaaaaaagcg gaaaatccga
    38041 ccgctttact ctttgcagcc ctttgccacg acttaggcaa aggcttaacg cctgctgata
    38101 ttttgcctca tcactacgga cacgaagtca aaggcattca accaacccga gagttagcca
    38161 atcggttaaa aatcccaact gagattaaag attttgccct tttagtgacc gaataccaca
    38221 cgcattgcca taaaatggcg gaacttcgcc ctgaaacggt aataaaactg tttaacagcc
    38281 tagatgtgtg gcgtaaacca aaccgctttt ttgatttcct gttcgcctgt gaagcggatg
    38341 ctagagggcg attaggtttt gaacagcgga attaccccca acctgaattg gcgaaaaaat
    38401 attaccaagc cgcaatcgaa attgatgtgc agcaagttat tcaagacggt tttgaaaaac
    38461 aagcaattcg ggaagagctg aataaacgca gagtgtatgc ggtaagaagc atcaagctag
    38521 atagcgacct aataaaccat taggttatcc gccactttct caaaatcaat tcccaaagtt
    38581 caagttggtt aaatgtatgg attctttaaa aggtggcgtt attttagtga atttataatg
    38641 ttcctatgct cttgattgtt gtagccacaa tttcagggta aagatcagca ataacccaat
    38701 gccgaaattg gcaatagtga agccggctaa cccaataaaa gataaaggtt caaattgata
    38761 aaattcaatc gcacctcttg ccattgcagc taaaccaaat gaaaatgccc agaaacccat
    38821 cgagaagcca ttttcagcta tccatttgag gtttcgtaaa ataaaacaga attgtaagaa
    38881 tccatatccc cataacattt ttgccaacaa gtctaattct ccacccatag aaaaataggc
    38941 agaagcgccc acaaatgccg gagcaagaac aattcctaaa gatggtctag caatcggaaa
    39001 aagcggacta agccgaattc tttgtaacat agtgggttca tatactaacc aggaaattat
    39061 gccaatgcct aaaaataaag aggcaatttc actgtagcca attaagctta atgaacttgc
    39121 catagtgaag ctccctgcaa ttggcggtag gtaatgtacc gggtgtagcg tttccggcaa
    39181 aaattgtgtg cctttccata atccaccaat aataaaaacc aagaaaatta aatgcacaat
    39241 gacgcctaaa aaaatcaata gttcgcctaa ataaaaatcc catacataga gcacatcacc
    39301 gcttatcatg gttgtcattg tcactaagga aaataaacaa cattgcactg ggtgcataaa
    39361 ttctgcttta atctgctcaa aacaaaaaaa cacacgatac acataacagc ctaataaggc
    39421 tacccaaagt atgactgacg ttccagcaat gatattacca attgttgtag cataaggagt
    39481 gataggcgtt gcatttaacc aggcaaggcc taacgccccc agccctaatg gcattgcaaa
    39541 aaagcctgta gttattggaa aaggtttaga ttcactcata aatttatcct catattgaaa
    39601 taatgcataa ctgtattcgg ctttataaca gtcatatgta aaaaatttta tttttctaac
    39661 cgcttgtaat aagaaaaacc taaagcactt gctttaggtt cctactacta ttcatactct
    39721 tctagccaga tagttaaaat cgcttcaaga atatgttcat ttgatgcttc cggatcatca
    39781 tcgaaatctt ccatttcaca aatccattta tgcatatctg taaaacgaac cgtggtcggg
    39841 tctaaatccg ggttcatatc atacagattt tcagcgatca ttcgggtatc tgtccatttc
    39901 attagtgcgc tgcctcattt gcgtgattta aattatattt tggaatttca acaaccaaat
    39961 cttcttcacc aacaatacat tggcaactta aacggctgtc tatttccaat ccccaagctt
    40021 tatccagcat atcttcttct tgatctgaag tatcatttaa cgaatcaaaa ccctcacgaa
    40081 ttaccacgtg gcaagtggta caagcacaag aacagtcgca agcgtgatga atttcaacgc
    40141 ctgcttcgtg tgctacttct aataaattat ctccggcttt agcttcaaca accatacctt
    40201 ccgggcaaaa ttcttcgtgt ggaagaaaaa caatttttgg cattttaaag tttccttatt
    40261 actaatccca ttcaatatta tccagatgtt cgccgatgaa gtttaaaatc tcttcctcat
    40321 tttcaaccag ataaacaata atcttcttta attcccttgc tttctcttta cttttctcgg
    40381 aaagcggata ggtaacataa taacatccta cgtccggatc ttcttctaat aactctaata
    40441 ggcttggacg ataaatacta agataagccc gtaaaaatgc agcccagtta taaccgttca
    40501 tataggcaac atcatattca gtattaatcc gctcgcccaa ctcaaaaatg cggttatcct
    40561 ctaaatcgaa ttcaaccgca acggttctga attgttctga aatctcggtt tttacatagt
    40621 tagacatact gcctccttat gctaaatcgt ccattttttt accggctaat gctttttgaa
    40681 ttgataaatt catacgccgt gctgcaaatt cttgcgtagc taaatccacc tctttgatac
    40741 cttgctgaat tgcaaagcga tcagtacctt cttttaattc gattaaacgg gctaattgtg
    40801 cttcaatcgc ttggaactcc tccacactca acactgcaga gccatcttct ttcaatgccg
    40861 ttaccacgct gtcaatcact cggtcggctt ctactcgctg ttcaactaac tggcgggctt
    40921 ccatatcttc tttggcattt gccattgagg atttaatcat ctgcgtaact tcttcatcgg
    40981 ttaagccata agacggtttg atttgaattg aagcctgcac tttggtcgat ttttccattg
    41041 cagtcacgtt taataaaccg tctgcatcaa cttggtaagt tacccgaacc tgtgcaaccc
    41101 ctgctgccat tgccggaatg ccacgcaaag tgattttacc gagagagcga caatcttcga
    41161 ctaattcacg ctcaccttgt aaaacgtgga ttgtcatcgc cgtttgaccg tctttaccgg
    41221 tagtaaactc ttgagctcga gctactggga tagtagtgtt gcgtggaata attttctcca
    41281 ccaaaccacc catcgtttca ataccaagcg aaagtggcac aacatcaagc aacaacatat
    41341 cggaatccgg tttgtttcca actaaaatat ccgcctgcac tgccgcacca atggcaacaa
    41401 ctttatccgg gtcgatagag gttaatggcg ttttaccgaa aaactcgccg acttgttcac
    41461 gcacaaacgg tacacgggtc gaaccgccta ccattaccac ttcacgaacc tcttctgctt
    41521 caactccggc atctttcaag gctcggcgac aagtcatcag cgagcgtttt acaagcggtt
    41581 gaattagctc attaaattgc gaacggctta ctacgcctat ccaatttgca aatttcactt
    41641 caaattcaac cgcttgcgaa agtgcgactt tggtttgagt tgccaaagtt aataattcac
    41701 gctgctcgct tgccgtttga ggtttgtaat ttgcctgttc agcaatccat tccgctaata
    41761 ggtggtcgaa atcatcgcca ccgagtgcgg tatcacctgc ggttgctaat acttcaaaca
    41821 cacctttgcg taaacgcaaa atagataaat cgaaagtacc gccacctaaa tcgtaaacgg
    41881 caatcacacc ttcttgtccg ctttctaaac cataagcaat cgctgctgcc gttggttcat
    41941 tgagcaaacg taatacattt aaacccgcaa gacgtgcggc atctttggta ctttggcgtt
    42001 gtgcgtcatc aaaataagcc ggtacggtaa ttaccacgcc tgaaagctca ccggctaaac
    42061 gcttttcggc aaattgtgcc aagtggctta aaatatccgc cgaaacttca atcgggcttt
    42121 tattaccttg cgctgtttgg attaaaggta aaccgttttc gctcgcgaca aattgataag
    42181 gtaaattttg ataacgttgc tgaacatctg ccagagaacg cccaatgaga cgtttagctg
    42241 aaatgacggt atttttcggg tcaagtgcag ctttggcaaa ggcttcaatc ccaactgttt
    42301 tttctgtttc cgcataatgc actactgacg gcactaatgc tctttcttgt tcatcaagca
    42361 atacctgagt ctgcccgcta cgcacagttg ccactaatga attggttgta cctagatcaa
    42421 ttcctaccgc taatttatgt tgatgtggtg cagcggtttg accgggttca gaaatttgta
    42481 ataatgccat ttatttatct ctttatcttt ctaacttagt tctctattgt attaaatgga
    42541 gtgaaatata ttgtttaaga gaagctgga
//
LOCUS       DANLUQ010000004        38716 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.004, whole genome shotgun sequence.
ACCESSION   DANLUQ010000004 DANLUQ010000000
VERSION     DANLUQ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 38716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38716
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.004"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..949)
                     /locus_tag="PXB27_000180"
     CDS             complement(<1..949)
                     /locus_tag="PXB27_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDL5345015.1"
                     /translation="MLNWAGKSTAYQTLQTPTSKTLTPCEEESVDFENTQNIFIEGEN
                     LDVLKALQKSYFNSVKMIYIDPPYNTGNDFIYNDSFADTKADYAEKVGDVDEHGKLKR
                     AFVRNSKENGHYHSNWLNMMLPRLHLAKNLLRDDGVIFISIDDNEQAQLKLLCDEVFG
                     EENFVAILSVENNPKGRKNSKFISVSNDFCIIYAKNKEMGKFVENIPKNAKDMCQDEN
                     GHYVHASGKRVLVGENKFNQPVTDFTSDKHYSVYFNKLDDDIILKFEDSLDDKDLNLI
                     NQGYVRYISFFDDEFVENTYTRFKFLELFEDESLEFTEDK"
     gene            complement(1382..2257)
                     /gene="ubiA"
                     /locus_tag="PXB27_000181"
     CDS             complement(1382..2257)
                     /gene="ubiA"
                     /locus_tag="PXB27_000181"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HDL5345016.1"
                     /translation="MNNETLFSKQKWIAYAQLMRFDKPIGTLLLLHPTLWALFIAAEG
                     MPPVAILAIFTLGVIVMRAAGCVINDYADREIDGHVKRTSQRPLATGRVTTTEAKVIF
                     AILLLFAFLLDLMLNRYAFLLSFVAVFLAVIYPFMKRFTHLPQVVLGMAFGWSIPMAF
                     GAVTETLPLECWLLFFANLAWTVAYDTQYAMVDRDDDLRIGVKSTAILFAQYDNKIIA
                     LLQFITLILLGLLGVVKDYHIGYFVVLALSATLFIYQCWLTKKRERSECFKAFLNNHY
                     FGLGVFVAILVGIYF"
     gene            complement(2250..3011)
                     /locus_tag="PXB27_000182"
     CDS             complement(2250..3011)
                     /locus_tag="PXB27_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HDL5345017.1"
                     /translation="MKQANRHKKIIELVNQLGYVSTEQLVAELNVSPQTIRRDLNEMA
                     ENNLIRRHHGGAAAPSNTENSDYTHRKQFFSHEKNAIAQQVAKLIPNGASLFLDIGTT
                     SEAVALALLSHKNLKVVTNNLNAAHILMQNEDCQITVAGGGLRSDGGLIGEETVRFIN
                     QFRLDFGILGISAVDMDGSMLDYDYHEVQVKRALMECSRQVVLVTDHSKFTRTAIARL
                     GNVKEVNYLFTDQDLPLELQTHLSQSDVIVKICNE"
     gene            complement(3078..4445)
                     /gene="purB"
                     /locus_tag="PXB27_000183"
     CDS             complement(3078..4445)
                     /gene="purB"
                     /locus_tag="PXB27_000183"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDL5345018.1"
                     /translation="MELTALTALSPIDGRYQDKVASLRPIFSEFGLLKFRVTVEVRWL
                     QKLASHTQINEVPVFSEKANDYLNQIVADFSLEDANRIKTIERTTNHDVKAVEYFLKE
                     KCEALPELQAVNEFIHFACTSEDINNTSHALMLKTAREEVLLPEWKKVIDAVAELAKC
                     YQHIPLLSRTHGQPASPTTMGKEMANVAYRLQRQYKQLENLEILAKINGAVGNYNAHL
                     SAYPEIDWHTFSQEFVESLGVTWNPYTTQIEPHDYIAEFFDCVARFNTILIDFDRDMW
                     GYIALNHFKQRTIAGEIGSSTMPHKVNPIDFENSEGNLGLANAVMAHLGQKLPISRWQ
                     RDLTDSTVLRNLGVGLGYALIAYASTLKGISKLEVNEQHLRDELNQNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDETAMREFIDKLEIPENEKNRLKEMTPASYIGYAVE
                     LVDKL"
     gene            complement(4479..5108)
                     /gene="hflD"
                     /locus_tag="PXB27_000184"
     CDS             complement(4479..5108)
                     /gene="hflD"
                     /locus_tag="PXB27_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HDL5345019.1"
                     /translation="MADYHDITLAYAGVCQAATLVQQFAHKGMADREAFKCALESLLI
                     TQPESTLSVFGDDLSHLKMGLETALAQTGGGNGKLDTEVGRYWISLLALSQKLNKNPE
                     AKQQLAQRLQQVERQLALYEDNVMADQMIANLAAIYSDIISPLGTKIHVMGMQDYLVR
                     PDIQNKIRASLLAGIRAGILWQQVGGSRWQFLFSRKKIFNQAQKFYRQI"
     gene            complement(5240..6904)
                     /gene="cydC"
                     /locus_tag="PXB27_000185"
     CDS             complement(5240..6904)
                     /gene="cydC"
                     /locus_tag="PXB27_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HDL5345020.1"
                     /translation="MKSLFPFFALYQTHFGRLLLGVVLAILGLAASIGLLSLSGWFLA
                     ASFLAGNALIFNFFYPSSGVRGLAVGRTISRYFERLVTHDATFRVLANLRASVFKKLI
                     PLSPSGLNRFRNSELLNRLVADVDTLDTLYLNLVSPFVSAVMIIAFMAIGLSFVSVPL
                     MLIICGTLLVLLAVIPTVFYRLGLKLGRNAIQSRANYRSQFIEWVQLNAEFLLFGNLN
                     QMSEKLQQTEQQWLNAQSKESRLSGLSNSLIMLSNGILTCVVIYLVSTSINVPTAKYP
                     EALIALVIFCVMASAEILSPIGIAFLHLGQVITAAERISEITEQQPNVTFGSKAEWQN
                     LSQNQPLVRCENVSFAYYGEQNVLNNLSFKVLKGQKVAILGKTGSGKSTIFQLLNRNY
                     DPTSGQIWLNNCKIDEYSEPMLRSKLVTLSQRVHIFSQTLKDNLLMGNAQATDEQMQE
                     AVRKVGLGHLLETDGLNLWLGEGGRPLSGGEQRRLGLARLLLSSAELVLLDEPTEGLD
                     RETEQQILNLILTHCCDRTLIMITHRQSGLDKFDVVYRIDNGRLVG"
     gene            complement(6904..8670)
                     /gene="cydD"
                     /locus_tag="PXB27_000186"
     CDS             complement(6904..8670)
                     /gene="cydD"
                     /locus_tag="PXB27_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HDL5345021.1"
                     /translation="MEKQRQRELQKWLKSQQKVIKKYMHLNILLGSFSSLCMIGQMWL
                     IATMLDKMLIGKQSPNSFLVEIMLLFCCFAARAFFIFLRERVGFKAGQTLRLHLRRQI
                     LAKMEAVGPMSIQQKPAGSWATLMLEQVENLHNFYARYLPQQFLSLIVPLLILCFVFP
                     INWAAGAILFATMPLLPLFMILAGMKAVEANQRNIGILSRISGQFLDKLKGLETIRLF
                     GQAEKQTEQIYQSTEDFRISTMDVLKMAFLSSAVLEFFTAVSIAVTAVYFGFIFLGEL
                     DFGYYGTGVTLFIGFFCLMVAPEFYQPMRELGVFYHDKAAAISAADNIETFLKEKVKT
                     QGGNLQENVENQPLVIQAKDCVIFSPQGKALTQALNFELNAHQHIALVGQSGAGKSSL
                     MNMLLGFLPYEGSVTINGTELRDLNLSQWRAKLAWVGQNPQLMHGSLKENILIGNPNA
                     TDSELAKALQLSKADEFVARLGLEHQVQDSNIGISGGQAQRIAIARALLRPYELLLLD
                     EPTASLDMDSEQQVLAALHNLSREQTTLMITHRVEDLTQCDEIWVMKQGQIIQKGRFT
                     ELEHTGFFAELIHNELVHQGEI"
     gene            complement(8785..12882)
                     /locus_tag="PXB27_000187"
     CDS             complement(8785..12882)
                     /locus_tag="PXB27_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB"
                     /protein_id="HDL5345022.1"
                     /translation="MTDENKQTATNQTDSAQEPQATQPKKSKWRFLKHGFCLTFILLL
                     LAILFLCTGYGQRKVLSWVDKFVEPLQLGQVQGSLQDGLQLSDARFVMDGVNVTVGQA
                     DLHIGFSCLLHYEMCLNNLSLKDTEVLIDTTKLPPSQPKEETEPFTELNLPLGISAQN
                     INLENIKVKVDDMDIHLNHFHTALSGKGRAITVHPTQLEGLNLLLALKLDETDTEQAV
                     DFAQKNAKTEALSATNPQEIADKVIQEAVEKTSEKVVEGFHQLQRKTVAQAEQVDNKV
                     DWAAIKAQLAQPLLDKNIRFSLPLDLNIEQIDIANVAIAQKAKDEKGNLIEPISLLNV
                     ASLQLQAQAKDQHINLKSLDFKSDQGDLTASGSLSLQDNYPLSWTLSGHEAKTAKVKL
                     PFNQIEASLSGELYDKTSLKVKTAGAISASLEGNVALAQAKTPFDLSLKSESMRYPFA
                     PQQNGDVLSLENIAMRLNGNLLDYALEAKVSAKGMGVPASSASLTGKGELTHFNIQDL
                     SLNTLEGTAKIDGLVDWSEGVAWRSNLKLQHINTKSLLADWPAVLSGSLSSEGYAGRG
                     NSASGWKADVSNIDIKGSLLQKNLHLSGNLKADHQQLLDVPGLSLVYGENKIDLKGVL
                     GEKSDFYADIKAPNLQGLVPNLKASVNGNAKLSGKVSEPNIDLDLVASNVGYEQFKLQ
                     NLTAKGKITTEKTIQGDLTLGLRQFAYNEIKVGNATLLLKGNEENHSLKLSAKGNPVG
                     ADLQISGKFDRLKEIWEGQLSQVSIQSTEFGQFQADKAVNVKYDNKAINANISAHCWH
                     NRKINLCFPTAFNAGVEGKVPFEIRNFDLAVLQQFLEKNSQLSGIVNAKGDAAWFKNK
                     QPQVNLDLTSNAIKFIQKMEGGKSFPLTVSPLKIDLKMSDNNLTLKSDLRVENNGRLS
                     TDLVMKDLSKARALSGNIHLDQLSLKLIRPLLERGDSVDGNVNARLTVSGTATSPLLH
                     GNLNVTELKARSVTMPFDITGGNLAMNFHGATSTLSGKLQTKQSELRLEGDADWRDLN
                     AWKTRVHAQANRFQVDIPNIAKVAVSPDIQVTATPTLLTLSGNVDIPWARIEVEQLPE
                     SAVSVSGDEVIMDGSVKNKVPFSQRNIPAQTSGGMAINADIKINIGNDVKLKAYGLNS
                     NVNGLLSVRQGKQGLGLYGQVHLREGRFAAYGQDLLIRKGDISFAGSPSQPTLDIEAI
                     RNPEAMEDPTITAGVRVTGLADNPTVKVFSDPAMSQNEALSYILTGRSLESSGDTSSS
                     NAVAAALLSMSLSKSSKLVGDVGSTFGLKDLSVTTAGIGDNTKVEVSASLAPKFRVKY
                     GVGIFAPLTELTLRYNLTPRLYLQWVSSINQAVDLMYRFEFDELF"
     gene            complement(12963..14798)
                     /locus_tag="PXB27_000188"
     CDS             complement(12963..14798)
                     /locus_tag="PXB27_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor"
                     /protein_id="HDL5345023.1"
                     /translation="MNTKLHFLTFCCLVAFASMAQANQEQRLSDVDRERVENLQEKLA
                     EDSDDEPEIEPLVTLEVKGIQGKDAKKNVELYLDQIANEYADGSERYQYLVQTTVDKA
                     LRALGYYNSRYQFVQLPQEGKKPLLVLNVELDKETVKIDETDIVLQGDAKTDEDFAHL
                     VSKAPKQGSRLNHKTYDNFKGDIESTAFSKGYFDGHWLYHRLEVYPTDHKADWRLGYD
                     SGKRYHYGQIRFVDSQIKEEYLQNILKIHSGEPYLANDLSILTQDYLSSNWFSSVMVE
                     PHLNEQDKLVDLNVLFQPKKRNEVEIGIGYETEVGPRLQLNWKKPWLNSRGHSIESRT
                     YVSKPEQTFEFGYNIPLREDPLHYYYQLSGTLENEDQNDTKSTAATLGLQRFWTHETG
                     WSFSVGLKARYDSFTQANDRFKTLLVYPTASLNRTRTDGKPYPLWGDTQKLTVNWGSR
                     AVGSDVNFYSYKASTSWARTYFDNHRFYLRAELGYLKANQFERIPPALRYFAGGDMSV
                     RGFGYKDISPRDPSNGKRVGGSHLATGTVEYQYQVYPNWWGAIFYDAGLASNKFKTQD
                     LYYGTGLGVRWASPIGAIKFDVATPVKSPDNSKGIQIYIGLGSDL"
     gene            complement(14810..15655)
                     /locus_tag="PXB27_000189"
     CDS             complement(14810..15655)
                     /locus_tag="PXB27_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HDL5345024.1"
                     /translation="MFKQINKRLNFNIFLQMFVLFTPLVQAAQMAIVIDDVGYRIKED
                     REILALPKAVSVAIIPVAPYATERAKDAYNQKRDILIHLPMEPKSKQPIEEGGIHIGD
                     NEEKIRKLIHTSRGQVPYAIGLNNHMGSGATSDSATMQHLLKVLKENTLFFLDSKTIG
                     SSVAAKTARQFGINTLERDIFLDDSDLLADVQKQFAHAINHARKNGVAVVIGHPRKNT
                     ISVLKQNLAQLPQDIELVSVGNLWRNEKTVPDKPFIMLFEQEPALSSVPPYDSIPLLR
                     GIPKE"
     gene            complement(15656..16819)
                     /locus_tag="PXB27_000190"
     CDS             complement(15656..16819)
                     /locus_tag="PXB27_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDL5345025.1"
                     /translation="MLVKRINSYCWTAIFSGVISFSTPFSFANNLNQIQQKIQQQTSK
                     INEQKQKRNALQSTLKNQEVEMGKVLDKLQKTEMSLTEVRQTIKATETEIARLEKLEK
                     QQKEKLKEQLDSAYRSGVNPSVLERLMSEEAKNAERMTAYYQHMNNIRIDAIVEIRKT
                     QADLKARRDELKGQQKDHQTQLNEQKKQEKDLKKVQSQRESTLRLIDKTLEQDQSRLE
                     NLRSNEQALKQQLEQAQREAQQAEKREQQREKQAKSGQSKTTENVERTPIKAGRYTMP
                     VSGNIITPFGNNWHGVVIGASAGTSVRAMASGRVIMAQWLAGYGNMVAIDHGNGDISL
                     YGYNQSISVSKGSRVQGGQVIAKVGNSGGQSRSALYFGVTRKGTPINPLNLVK"
     gene            16987..17670
                     /locus_tag="PXB27_000191"
     CDS             16987..17670
                     /locus_tag="PXB27_000191"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011201466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDL5345026.1"
                     /translation="MQLVFIRHGFSEWNAKNLFTGWRDVNLTERGIEEAKSAGQKLKA
                     AGFEFDIAFTSVLTRAIKTCNIVLEESDQLWIPQVKNWRLNERHYGALQGLDKKETAE
                     KYGDEQVHIWRRSYDISPPDLDPQDPNSAHNDRRYANLPKDLIPNAENLKITLERVLP
                     FWEDHIAPALISGKRVLVTAHGNSLRALAKHIEGISDADIMDLEIPTGQPLVYELDDN
                     LKVISKRYL"
     gene            complement(17807..18364)
                     /gene="frr"
                     /locus_tag="PXB27_000192"
     CDS             complement(17807..18364)
                     /gene="frr"
                     /locus_tag="PXB27_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDL5345027.1"
                     /translation="MINEIKKDTQDRMEKSLEALKGHIAKIRTGRAQPSLLDGIQVEY
                     YGSATPLRQVANVVAEDARTLAVNVFDKSLISAVEKAILTSDLGLNPSSAGTTIRVPL
                     PPLTEERRRDLIKIVKAEGEQGKVAIRNVRRDANDQIKALLKDKEISENEQHKAEEDI
                     QKITDTFIKKVDEVLAAKEKELLDF"
     gene            complement(18416..19132)
                     /gene="pyrH"
                     /locus_tag="PXB27_000193"
     CDS             complement(18416..19132)
                     /gene="pyrH"
                     /locus_tag="PXB27_000193"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDL5345028.1"
                     /translation="MSKPIYKRILLKLSGEALQGSEGFGIDPSILDRMALEIKELLAE
                     GVEVGVVLGGGNLFRGAKLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAEVNAKLM
                     SAFQLNGICDTYNWSEAIKMLREKRVVIFSAGTGSPFFTTDSAACLRGIEIEADVVLK
                     ATKVDGVYDKDPAKYADAKLYNKLTYAQVIEQELQVMDLAAFTLARDHGMPIRVFNMG
                     KPGALRNVVFGTEEGTTISE"
     gene            19224..20786
                     /locus_tag="PXB27_000194"
     CDS             19224..20786
                     /locus_tag="PXB27_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDL5345029.1"
                     /translation="MKSKQLFGILVAISLATAASCLMLIGSGFFPNPSIFEVILGVFL
                     IIALASSKWTYYLLLLPISIAYAIYTPVGIKFGKPTYEYIASIFATDMMESKEFIAQL
                     PILNIAAGIAIVLAVIFYRKITNRYQIRFLRNKTFVISAFVLMLFSLAPFKFFQEFYN
                     SSLKVKKELEVLNNLTLDSQWGQSTLSSTSNYDDYVLIMGESARKDYHHAYGYPAANT
                     PFMSSAKGTLIDGLTSGGTNTIASLRLMLTKPDTEKWEANYGLTLIDLIKSTGIKTYW
                     ISNQGYLGNYDTPISSIGNKSDVKIFTKAGDSLNTNVSDFELLPHFAKVIEEPAQGKR
                     FIFLHLYGSHPITCDRLTDYPKMFDDDKLPKRYFNVNCYVSSIKKTDELLKRVYDLLV
                     KNQENHQRSFSMVYLSDHGLSHEVSKENIVIHNGKDRSKLHYDIPLFKISSDDTQRNE
                     YKAFKSGLNFTNGIANWIGIENPLLDKEINLFNEQNDKSDYGLKKIIETQSTVEDPAV
                     PIPESYLYKATN"
     gene            complement(20850..21701)
                     /gene="tsf"
                     /locus_tag="PXB27_000195"
     CDS             complement(20850..21701)
                     /gene="tsf"
                     /locus_tag="PXB27_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDL5345030.1"
                     /translation="MAEITASLVKELRERTGAGMMECKKALVEANGDIELAIDNMRKS
                     GQAKAAKKAGRVAAEGVILARVASGFGVLVEMNCETDFVAKDAGFLELANEVADFAVA
                     NKGTTIEALQAQFEEKRAALVAKIGENMNIRRVQYLEGEVIAQYLHGAKIGVLVAGAG
                     SEDELKKVAMHVAASKPEFVNPEDVSADVVAKEREIQIEIAMNSGKPKEIAEKMVEGR
                     MAKFTGEVSLTGQPFVMDPSQSVGQYLKSVNTSVSNFIRLEVGEGIEKVEEDFAAEVA
                     KITGGNA"
     gene            complement(21797..22516)
                     /gene="rpsB"
                     /locus_tag="PXB27_000196"
     CDS             complement(21797..22516)
                     /gene="rpsB"
                     /locus_tag="PXB27_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005645552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDL5345031.1"
                     /translation="MAQVSMRDMLNAGVHYGHQTRYWNPKMKPFIFGARNGVHVINLE
                     KTLPLFNEALAELTRIASNNGKVLFVGTKRAASDAVKAAAVESQQFYVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQTQDGTFDKITKKEALMRTRELEKLELSLGGIKDMNGLPDA
                     IFVIGADHEHIAIKEANNLGIPVFAIVDTNSTPDGVNYIIPGNDDATRAIQLYLDSAV
                     AAIKEGRGVETVEVAEEASAE"
     gene            22861..23889
                     /locus_tag="PXB27_000197"
     CDS             22861..23889
                     /locus_tag="PXB27_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL5345032.1"
                     /translation="MKKTLSALAIAAATFTSTSTLAANEVNVYSYRQPYLIEPMLKNF
                     EKDTGIKVNIIFADKGLVDRVKQEGELSPADVLLTVDISRVMEIVNADLAQKIDSKVL
                     EKNIPAQFRDSNDQWFGLTTRARVIYTSKDRVGKLPAGFDYLDLAKPEYKGKVCVRSG
                     KNSYNVSLFAAMIEHYGIEKTKAFLEGLKANLARKPQGGDRDQVKAIKEGICDYSIGN
                     SYYYGKMLDDEKQKSWAEAAIINFPSGEHGTHKNISGVVIAKHSPNKANAVKLIEYLS
                     GEKAQGLYAELNHEYPVKEGIEPSAIVKGWGTFKSDTIKLEDIAKNYEAALKLVDEVK
                     FDDFSEKK"
     gene            24012..25658
                     /locus_tag="PXB27_000198"
     CDS             24012..25658
                     /locus_tag="PXB27_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL5345033.1"
                     /translation="MRSQFCWKSIAALTTFIVLLPLIAICYHALQGDNENLAHLWQTM
                     LPIYLKNSTLLVLGTVFLALIFALPTAWIVANYRFWGQRTLQWLLCLPLAMPAYLIAY
                     LYTDLLDYSGAFQGWLRSIFGWTHPQDYWFPQIRTLYGACFVLALVLYPYIFLLVRVA
                     LLEQSENLTHSAKMLGASPSQLFRRITLPLIRPSVAVGIALVAMETLGDFGTVAYFAV
                     PTLTTAIYDSWLGYHDLGTASQIAIFMLLMIFLFLSLEQYSRRKQKSYQRGYEKKSAF
                     KFLTGWKLALALAWCWGLLALAFFIPFGRLLYWAYDYFEQAWNLDFVQFSLNSLKVSI
                     IASVITVLIALLLHFSKRLNTRKILVKNDRLSLQLASLGYAVPGTVLAIGLLSPLTFA
                     DHQLHAWLKSLELSPVGLIFSGSLFALVLAYTIRFLAMAIGSLETSLNKISPSLDMAS
                     QTLGKNGSAMLVKIHIPLIYKGILTAGLMVFIESMKELNASLLLRPFNFDTLATYVFT
                     FTSDEQLERAALPAIVLVLVGLLPVIWLTRSLISQSEKER"
     gene            25668..26678
                     /locus_tag="PXB27_000199"
     CDS             25668..26678
                     /locus_tag="PXB27_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5345034.1"
                     /translation="MSVLTINHLNLNFGSTKVLQNLHLAVNQNEIICLLGASGCGKTT
                     LLKAIAGLLPIEQGDIHLAGECLKSKAVEERQIGLIFQDYALFPHLTVADNIQFGLSK
                     LPKVQQKAITEKMLSVVHLQGFESRFPHELSGGQQQRVAIARALACQPELLLLDEPFS
                     NIDSQTRYAMIQEIKQILKSQNVPAIFVTHSKEEAFAFADKIAVMDQGKIIQFGKPNE
                     LYHSPVNPFVADFLGGTNYLNCNVTEDNVLCSPIGEFRLFPEMNVQKGRYRWLLRPEQ
                     IILKPNELGLGKVVGKLFLGLYYRYQVEIKGIEIVTYQSQELKIGEQVEVGFQVREFI
                     LF"
     gene            26688..27314
                     /locus_tag="PXB27_000200"
     CDS             26688..27314
                     /locus_tag="PXB27_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HDL5345035.1"
                     /translation="MKIEVWSDYACPFCYIGKRHLEQALAQFEGEVEVVFRAFELDPH
                     ANGEPEGDIQQRLMRKYQKTAEQADEMIRYVEQAGKQAGLDLRYRTTQYTRTFEAHRL
                     AKFAESKDLGEAMVERLFKAYFTDNTILAKRTELISLALDIGLERDEIAQLLTGDDFG
                     HEVREDERVAHKYGIHSVPFFVINEKLGVSGAQPPEILLDAIKQALQK"
     gene            complement(27376..28866)
                     /locus_tag="PXB27_000201"
     CDS             complement(27376..28866)
                     /locus_tag="PXB27_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HDL5345036.1"
                     /translation="MSLYQIARTETGKVISLNAKMANRHGLIAGATGTGKTVTLRKLA
                     ESLSDDGVPVFLVDVKGDLSGSVQAGNFSGKIAERILQFNLGGEDYLKGYPVSFWDVF
                     GTSGIPLRITISEMGPMLLSRLLNLNDTQGGLLNLVFRVADDRGLLLIDLKDLRALLK
                     FVADNAKEFQLEYGHISAASVGAIQRALLRLENEGADNLFGEPALDLHDWIQTRNGRG
                     VINMLNAEKLINSPRVYSAFLLWFMAELFENLPEVGDPEKPTFVLFFDEAHLLFDDAP
                     KALVDKIEQVVRLIRSKGVGVYFVTQNPLDIPDSVLGQLGNRIQHALRAFTPRDQKAV
                     KSAAETFRANKEVNVVEAITQLGVGQALVSVLDEKGMPTPVEIAYIYPPKSQLAPLAT
                     GERDSLVKQDDLYHHYSQYVDNESAFELLNAKAEAIKAEQAAAEEANNDFFGGILSSI
                     FGSKKKKDQSITEQVVSQVAHSVGRNLRNQVTKQIMRGILGAITKK"
     gene            complement(28925..30160)
                     /gene="rhlB"
                     /locus_tag="PXB27_000202"
     CDS             complement(28925..30160)
                     /gene="rhlB"
                     /locus_tag="PXB27_000202"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HDL5345037.1"
                     /translation="MSNHLTEHRFIDFPIHANVLNALDSKGFEFCTPIQAKTFPITLK
                     GKDIAGQAQTGTGKTLAFLIATFNHLLNNEIETSKNQPRALILAPTRELAVQIGADAE
                     LFLKHSDLKLALAYGGDGYDKQVQEIEKGVDILVGTTGRVIDYVKQGIINLDKIQVIV
                     LDEADRMFDLGFIKDIRYLMRKAPKPSERLTLLFSATLSAKVRELAYEDMDNAEYIEI
                     EPLQKTGHRIKEELFYPSNEDKMALLLTLLEEEWPERCMIFANTKHKCEEIWQYLSAD
                     GHRVGMLTGDIPQKKRLSLLDNFTKGNLDILVVTDVAARGLHIPDVTHVFNYDLPDDK
                     EDYVHRIGRTGRAGESGHSISFACERYAVNLPAIEEYIGHQIPVSQYDKESLLTLPKV
                     NRSRSKKTFLAKTKKNSRN"
     gene            complement(30248..30970)
                     /gene="recO"
                     /locus_tag="PXB27_000203"
     CDS             complement(30248..30970)
                     /gene="recO"
                     /locus_tag="PXB27_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDL5345038.1"
                     /translation="MTIDQWQRAFVLHRREYSENSLLVEFFTEHHGRITLLAKGARRA
                     RSPLKAVLQPFTPLLLRWSGKGDLKTLTKAEPAALTLPMETLSLYSGFYINEVLARVL
                     ENQTAYPELFQHYLRCITELATQKQIEPILRTFEFHTLKALGYGVDFTHCAATGLPVD
                     PKMTYQFQENQGFIASLLQNNQSFLGSDLLAFAQLDFSEKSTQQAAKRFTRMALKPYL
                     GSQPLKSRELFQSILPNKLKSG"
     gene            complement(31200..32117)
                     /gene="era"
                     /locus_tag="PXB27_000204"
     CDS             complement(31200..32117)
                     /gene="era"
                     /locus_tag="PXB27_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013525392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDL5345039.1"
                     /translation="MTEQIQTPAKTYCGFIAIVGRPNVGKSTLLNKILGQKISITSRK
                     AQTTRHRIVGIHTEDQYQAIYVDTPGLHIEEKRAINRLMNRAASSAIGDVDLIIFVVE
                     GTKWTEDDEMVLNKLRSAKAPVLLAINKIDNIKEKEELLPHITELSQKFDFAEILPIS
                     AQRGKNVHILQKFVRQSLRQGEHHFPEEYVTDRSQRFMASEIIREKLMRFTGEELPYS
                     VTVEIEQFKMNERGVYEINGLILVERDGQKKMVIGAGGQKIKTIGIEARKDMERLFDN
                     QVHLELWVKVKAGWADDERALRSLGYMDE"
     gene            complement(32220..32894)
                     /gene="rnc"
                     /locus_tag="PXB27_000205"
     CDS             complement(32220..32894)
                     /gene="rnc"
                     /locus_tag="PXB27_000205"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDL5345040.1"
                     /translation="MQLERLQKKLGYQFHNLDYLIQALTHRSAATKNNERLEFLGDSI
                     LNFTIGKVLYDKFPKANEGELSRMRATLVREQTLAIIARKFDLGDYLKLGPGELKSGG
                     FRRESILSDCVEAIIAAIYLDQDMAKAMEKVEDWYSDLLKDISPGDSQKDPKTRLQEF
                     LQGRKLPLPEYDVLDIKGEAHNQTFRVTCKVINVEEVFIGIGTSRRKAEQNAAEKALA
                     VVKTKK"
     gene            complement(32940..33899)
                     /gene="lepB"
                     /locus_tag="PXB27_000206"
     CDS             complement(32940..33899)
                     /gene="lepB"
                     /locus_tag="PXB27_000206"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL5345041.1"
                     /translation="MAQFMPIIFLGIIYGIWQWLDSMQLPNTISIILVALVIICGGFW
                     AFYKFSADPRRKQAIVLQQKRLGRELTAEEIAEIQPKSAVGEFFASLFGILFFVTVLR
                     SFLFEPFQIPSGSMEPTLRVGDFLVVNKFSYGIKDPIWQNTLIETGKPERGDVIVFKA
                     PAQPHVDYIKRVVAVGGDKIKYDFATQQLTVTKANGETKVFEYSEGKPNPEFFYHGEM
                     QVERTEKGNVTHQILNHPMAFNYAPYFYQQEGQAEGEWVVPQGYYFVMGDNRDNSEDS
                     RFWGFVPERNIVGKATFIWLSLDKKANEFPTGLRFSRMFTSIN"
     gene            complement(33986..35779)
                     /gene="lepA"
                     /locus_tag="PXB27_000207"
     CDS             complement(33986..35779)
                     /gene="lepA"
                     /locus_tag="PXB27_000207"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDL5345042.1"
                     /translation="MQNIRNFSIIAHIDHGKSTLSDRLIQTCGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLNYKAKDGEIYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAIEIDLEVVPILNKIDLPAAEPERVAEEIEDIVGIDAIDAVR
                     CSAKTGLGIEDVLEEIVKKMPAPEGDAEAPLQALIIDSWFDNYLGVVSLVRVKNGVLK
                     KGDKIKVMSTGQSYNVDRLGIFTPKQVDTTELKTGEVGWVVCAIKDILGAPVGDTLTH
                     HHNPATEVLPGFKKVKPQVYAGLFPISSDDYEAFRDALGKLSLNDASLFYEPETSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVVQTSGETIYVDSPSKLPA
                     INNIAEIREPIAECNMLVPQEFLGNVITLCVEKRGVQTNMVYHGNQIALTYEIPMGEV
                     VLDFFDRLKSTSRGYASLDYGFKRFQTADMVRVDIMINGERVDALAIIVHRANAAYRG
                     RELVEKMRELIPRQQFDIAIQAAIGNHVIARSTVKQLRKNVLAKCYGGDVSRKKKLLQ
                     KQKEGKKRMKSLGNVEVPQEAFLAILHVGKD"
     gene            36052..37281
                     /locus_tag="PXB27_000208"
     CDS             36052..37281
                     /locus_tag="PXB27_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HDL5345043.1"
                     /translation="MQFREINPSPRLLMCPGPTDADPRVLRAMSAPILGQFDPEFTSL
                     MNETMEMERRLFQTKNEQTYVVNSTSRGGLETVLTAAICPGDKVLIPAFGRFGYLLNE
                     ILARSGADITIIEREWGTVFEPEEIEAELKKGGYKAVAIIHGETSTSMMQPLEEIGKI
                     CKQYDAMLIVDTVATLGGVDIRVDEWGIDACIGGTQKCISAPSGTALITYNKRIEEII
                     AKRKRVEKGIRAESDVDGTLPPIPSNYLDLAQLQDYWSPRRLNHHTETTSAQYGVHEA
                     LRIILQEGLEARFARHILNDKALCAGLEAMGLKIFGDRKHKAPVVTAFHIPEGMSGPA
                     LRSDILNHFGIELATSFGPLDGKVIRIGNMGFSSQKRNVLLTLGALEAVLLSHKVKVP
                     SGDAVAAALAVYKEADK"
     gene            37475..>38716
                     /locus_tag="PXB27_000209"
     CDS             37475..>38716
                     /locus_tag="PXB27_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL5345044.1"
                     /translation="MPNFSFTHLYKKHTKELPTVTLLSALIGLSQVSYANPLPLGNTT
                     GTNNLAVGEGSFALGTGSQAIGKNAIATGGNISPQEFNVELENFRDLVNQIEELRTQI
                     AEQEKQGSVNQQLQDALKSQIAQYDTILERVREKQAQQAILANSVISMRKELVNRRNQ
                     LTELENNFNTSFLTGKNKQEAYQKFSTVIESLDWDKLSKTNGVDQLATDLKTKIETDF
                     PNINFSNEKYKDLIKGYSNIQGNLVTAIPNFLEKVNNDFAISARNRDLYSHYLPYQGI
                     DDWENSNFNFRTLNGVYYDDNLTSTELLRKFMIQRDYNTLYALSSARTGNADSLAKNY
                     HLVVAKMSYENLKKELSKKPDSTFNKLKKLYETNGEFLNEYILDNFRGNPLELLTVPA
                     LKESLENQESFPSRLYQKFNIF"
BASE COUNT        11257 a         8319 c         7143 g        11997 t
ORIGIN      
        1 ttttatcttc tgtgaattct aaagattcgt cttcaaataa ctcaaggaat ttaaatcttg
       61 tgtatgtgtt ttctacaaat tcatcatcaa aaaacgaaat atatcttaca tacccttgat
      121 taattaaatt taaatcctta tcatcaagag aatcttcgaa ctttaaaata atatcatcat
      181 ctaatttatt aaaataaact gaatagtgtt tatctgaggt aaaatctgtt acaggctgat
      241 taaatttgtt ttccccaact aatactcgtt ttccacttgc gtgaacataa tgtccatttt
      301 catcttgaca catatcctta gcatttttag gaatattttc aacaaatttc cccatttctt
      361 tatttttagc ataaataata caaaaatcat tacttacaga aataaattta gaattctttc
      421 tacctttcgg attattttca actgataata tggcaacaaa attctcctcc ccaaacactt
      481 catcacaaag caatttcagc tgtgcctgtt cgttatcatc aattgaaata aaaatcaccc
      541 cgtcatcacg cagtaaattt tttgcgagat gcaaacgagg caacatcata ttcagccagt
      601 tactgtgata atgcccattt tctttgctgt tacgaacaaa ggctcgtttc aatttgccgt
      661 gttcatcaac gtcgcccact ttctccgcat aatctgcttt ggtatcggca aaactgtcgt
      721 tataaataaa atcgttgcct gtattgtatg gcggatcgat ataaatcatt ttgaccgaat
      781 tgaagtagga tttttgcagg gcttttaata catctaaatt ttcaccctca ataaagatat
      841 tctgcgtatt ttcaaaatcc acactctctt cctcgcaagg agtgagcgtt ttgctggttg
      901 gggtttgtaa ggtttgatag gctgttgatt ttcctgccca gttgagcata tagcggtcag
      961 gttcggtgga aaaattatcc gaaaaaagtt gttggaattt gtcgaagtcg attttacctt
     1021 cggtgaaaat ttcaggtaaa agggatttta attgagaaag ttgttgatct ttaagcgaaa
     1081 agttggcaag gctgtgctgt gctgtgctgt gctgtgctgt gctgtgctgt gctgtgctgt
     1141 gctgtgctgt gctgtgctgt gctgtgctgt gctgtttggc attgtaatgt cccttttttg
     1201 aatgtctact acaaaatggg atattttaca aaccgattga ctaatgaagc aagaatataa
     1261 ttaactacaa ctggatatat tttgcaaatt tcccgataaa tttcaccgct tgtagataaa
     1321 aaccgtagat aaaaaccata gataaaaaat aggagctaaa catttttagc ccctaccgtc
     1381 atcagaaata aatcccgact aaaatcgcca cgaacacacc taagccgaaa tagtggttat
     1441 ttagaaacgc tttaaagcat tcgctacgtt ctcttttttt ggtcagccaa cattggtaga
     1501 taaacagcgt ggcacttaag gctaatacga caaaatagcc gatgtgataa tcttttacca
     1561 cgcccaataa ccccaataaa atcaaggtga taaactgtaa taacgcaata attttattat
     1621 cgtattgagc gaataaaatt gcagtggatt taactccaat tcgtaaatca tcgtcacgat
     1681 caaccattgc atattgggtg tcgtaggcga ctgtccacgc tagattggca aaaaacagca
     1741 gccagcattc aagtggaaga gtttccgtta ccgcaccaaa tgccatcgga atcgaccaac
     1801 cgaatgccat tccaagcact acttgtggca agtgggtaaa acgcttcata aagggataaa
     1861 tcaccgccag aaaaactgcc acaaaggaaa gcagaaacgc atagcggttt agcattaaat
     1921 cgagcagaaa agcgaacaac agcaagatgg caaaaatgac ttttgcctca gtggtggtca
     1981 ctctgccggt agctaatggg cgttgagagg tgcgtttaac gtgtccgtca atttctctat
     2041 cggcataatc attaatcaca caacctgccg cacgcataac gattacccca agggtaaaaa
     2101 tggctaaaat cgcaacaggc ggcattccct ctgcggcaat aaataacgcc catagtgtgg
     2161 ggtgtaataa taataacgtg ccgatgggtt tatcgaaacg cattaactgg gcgtaggcaa
     2221 tccatttttg tttagaaaaa agagtttcgt tattcattac aaattttgac gataacgtcc
     2281 gattggctta aatgagtctg taattctaac ggtaaatctt gatctgtaaa caaataattt
     2341 acttctttaa catttcctaa acgtgcaata gctgtacggg taaactttga atgatcggtc
     2401 actaaaacca cttgtcggga acattccatt aatgcacgct ttacctgcac ttcgtggtaa
     2461 tcgtaatcga gcatagagcc gtccatatcc accgcactaa tgcctaaaat cccaaagtcc
     2521 aaacggaatt ggttaataaa gcgaaccgtt tcttctccga ttagcccgcc atcacttctt
     2581 agccctccgc ctgctaccgt aatttggcaa tcttcatttt gcatcagaat atgagccgca
     2641 tttaaattat tggtaactac ttttaaattt ttatgcgaaa gtaaggctaa tgccactgct
     2701 tctgaagtcg tgccaatatc taaaaataat gatgctccat ttggaatcag ttttgccacc
     2761 tgttgggcaa tcgcattttt ctcgtgtgag aaaaattgct tgcggtgggt gtaatcacta
     2821 ttctccgtat tggaaggagc ggctgcccca ccgtgatgac ggcgaatcag attattttct
     2881 gccatttcat tcagatcacg gcgaatggtt tgcggactca cattcaattc agccactaat
     2941 tgctcggtac tcacatagcc aagctgatta accagctcaa tgatcttttt gtgtctgttt
     3001 gcctgtttca tatttcctct tttgcaaata atttgcagaa aaagaccgct tgcaagcggt
     3061 cttttttcag ttaaatttta caatttatca acaagttcaa ccgcatagcc gatatagctt
     3121 gccggtgtca tctctttcaa acgatttttt tcattttccg gaatttccaa cttatcgata
     3181 aactcacgca ttgcggtttc atcaacccgt ttgcctcgag tcagctcttt aagtttttca
     3241 tacggttttt caatgccata acgacgcata acagtttgaa ttggctctgc caatacttcc
     3301 cagttttggt tgagttcatc acgcaagtgt tgttcattca cttctaattt gctaatacct
     3361 tttaaggtgg acgcataagc aattaacgca taacctaaac ctacacctaa attacgcaac
     3421 acggtggagt cagttaagtc acgctgccag cgagaaatcg gcaatttttg ccccaaatga
     3481 gccattaccg catttgccaa gccaagattg ccttcggaat tttcaaaatc aatcgggttc
     3541 actttatgtg gcattgtgct agagccgatt tcaccggcaa tagtgcgttg tttgaagtga
     3601 tttaaggcaa tgtagcccca catatcacga tcaaaatcaa ttaaaatcgt attaaaacga
     3661 gccacgcaat caaagaactc cgcaatataa tcgtgcggtt caatttgggt ggtgtatggg
     3721 ttccacgtca cacctaacga ttccacaaat tcttggctga aagtgtgcca atcaatctcc
     3781 ggatatgctg ataaatgtgc gttatagttg cccactgctc cattgatttt agcgagaatt
     3841 tctaaatttt ctaactgttt atattggcgt tgtaaacgat atgccacatt tgccatctct
     3901 ttacccattg tggtcgggct tgccggctga ccgtgtgtgc gagaaagcaa cggaatatgt
     3961 tggtaacatt tcgccagctc tgctacagca tcaatcactt ttttccattc aggtaataat
     4021 acttcttcac gagcggtttt aagcattaaa gcgtgcgaag tattattgat atcttcagaa
     4081 gtacaagcaa aatgaataaa ttcattcacc gcttgtaatt caggcaacgc ttcacatttc
     4141 tcttttagga aatactccac cgcttttaca tcgtggttgg tggtacgttc aatcgtttta
     4201 atccgattgg catcttctaa cgaaaaatca gccacaattt ggtttaggta atcgtttgct
     4261 ttttcagaaa acactggaac ttcattaatt tgtgtatgcg atgccaattt ttgtaaccag
     4321 cgaacttcca ccgtcacacg gaattttaat aagccaaatt cactaaaaat cggacgaagg
     4381 ctcgcaactt tgtcttgata acgtccatca atcggggata acgcagttaa agcggtaagt
     4441 tccattgttt tatattctcc aaataggtaa atgttcggtt agatttgacg ataaaatttc
     4501 tgtgcttggt taaaaatttt cttgcgtgaa aataaaaatt gccaacggct tccgccgact
     4561 tgctgccaca aaataccggc tcgaataccg gctagtagcg aggctcgaat tttattctgt
     4621 atatccgggc gaaccaagta atcttgcatt cccatcacgt gaattttcgt acctaacggg
     4681 ctgataatat cgctataaat agcggctaaa ttggcaatca tttgatctgc catcacatta
     4741 tcttcataca gggctaactg gcgttccact tgctgtaaac gctgggctaa ctgttgtttt
     4801 gcttcagggt ttttattgag tttttggcta agggcgagca ggcttatcca ataacgtccg
     4861 acttcggtat ctaatttgcc gttaccgccg ccagtttgag ctaatgcggt ttctaagccc
     4921 atttttaaat gggaaagatc atcgccaaat acacttaacg ttgattccgg ctgagtgatt
     4981 aacaggcttt ctaaagcaca cttaaaagcc tctctgtctg ccatgccttt atgtgcaaat
     5041 tgttgcacta acgtcgccgc ttggcaaacg ccggcataag caagggtaat atcgtggtaa
     5101 tccgccatct ctgtaaaatt caatatgata aaacattagt gggaatatag cattttttca
     5161 gcttcttttg aatggcaaaa ggcatttatt ctattgaaaa ataaatgcct tttcattaat
     5221 acgcaatttg ctgcaaaaac tatcctacca atcgcccatt atctattcga taaaccacat
     5281 caaatttatc cagtccgctt tggcggtgag tgatcataat taaggtgcga tcacagcagt
     5341 gagtcaaaat taggtttaaa atctgctgtt cggtttctct gtccagtcct tctgttggct
     5401 catcaagtaa gactaattca gcagaactca ggagcaaacg ggcaagccct aaacggcgtt
     5461 gttcgccacc agaaagcggt ctgccgccct cgcctaacca taaatttaag ccgtctgttt
     5521 caagcaaatg ccctaagcct acttttctta ctgcttcttg catttgttcg tctgtagctt
     5581 gagcattccc cattaataaa ttatctttta gggtttggct aaaaatatgc actcgttggc
     5641 ttaaggtaac taattttgag cgtaacatcg gctctgaata ttcgtcaatt ttacaattat
     5701 tcagccaaat ttgaccgctt gtagggtcgt agttgcggtt taacagttgg aatatggtcg
     5761 attttccgct acccgtttta cctaagattg ccactttctg cccttttaat accttaaagg
     5821 aaagattatt tagtacgttt tgctcgccgt aataagcaaa gctcacgttt tcacagcgta
     5881 caagcggttg attttggctc aaattttgcc attctgcctt gctaccaaat gttacattag
     5941 gctgttgttc ggtaatttca ctaatccgct cggcggcggt aatcacttgc cctaaatgca
     6001 aaaaggcgat accaatcggg gaaagaatct ccgccgatgc cattacacag aaaatcacta
     6061 acgcaattaa ggcttcaggg tattttgcag tgggcacatt aatcgaagtt gaaaccaaat
     6121 agatcaccac acaagtcaaa atgccgttgg ataacattat caagctattt gataagccgg
     6181 acagtcggct ttctttgctt tgagcgttca gccattgttg ttcagtttgc tgtagttttt
     6241 cgctcatttg gttcaagttg ccaaagagta aaaattctgc atttaactgt acccactcaa
     6301 taaattggct gcggtaattt gctcggcttt gtattgcatt acgccctaat ttcaaaccca
     6361 atcgataaaa tacggtagga ataacggcaa gcaataccaa tagtgtgccg caaatgatca
     6421 gcattaatgg cacggaaaca aaagaaagcc cgattgccat aaatgcaata atcatcactg
     6481 cactgacaaa aggcgagact aaattcagat atagggtgtc taacgtatcc acatctgcca
     6541 ccaagcggtt taacaattcg ctgtttctaa agcgatttag cccgcttgga ctcagtggaa
     6601 tcaatttttt aaagacgcta gcacgcaaat tcgccaatac ccgaaaagtc gcatcgtggg
     6661 tgactaaacg ttcaaagtaa cgtgaaattg tccgccctac tgccaaccct ctcacccccg
     6721 atgaagggta gaaaaagtta aaaataagcg cattgccggc tagaaatgag gcagctaaaa
     6781 accaaccgga aaggctgagc aaaccgatac ttgccgctaa tcctaaaatt gccagcacaa
     6841 cacctaataa tagtcgccca aaatgggttt ggtagagagc aaaaaacggg aacaacgatt
     6901 tcattagatt tctccctgat gcactaattc attatgtatt aattccgcaa aaaagccggt
     6961 gtgttctaat tcagtgaaac gacctttctg aatgatttgc ccttgtttca tcacccaaat
     7021 ttcatcgcat tgggttaaat cttccactcg atgagtaatc attagcgttg tttgctctcg
     7081 gctgagatta tgcagagcgg ctaacacttg ctgttccgaa tccatatcaa ggcttgcagt
     7141 cggctcgtct aacagtaata attcgtacgg gcgaagcaac gctcgtgcaa tcgcaatccg
     7201 ctgtgcttgc ccgccagaga taccgatatt actgtcttgc acttgatgct ccaaccctaa
     7261 acgtgccaca aattcatcgg cttttgaaag ctgtaatgcc ttggctaatt cagaatctgt
     7321 cgcattaggg ttgcctatta aaatattctc tttcaaactg ccatgcatta attgcggatt
     7381 ttgccccacc cacgctaatt tcgctcgcca ttggcttaaa tttaaatctc gcagttcggt
     7441 gccgttaatc gtaaccgacc cttcataagg caagaaacca agtaacatat tcattaatga
     7501 actttttcct gcaccacttt gcccaaccaa agcgatatgt tggtgtgcat tcaactcgaa
     7561 atttaacgct tgggttaagg ctttgccttg tggtgagaag atcacgcaat ctttggcttg
     7621 aatcacaagc ggttgatttt caacattttc ttgcaaattg ccaccttgcg ttttcacctt
     7681 ttcttttaag aaagtttcga tattatctgc ggcactaatc gctgctgcct tgtcgtgata
     7741 aaacacgcca agttcacgca tcggttggta aaactcaggt gccaccatta gacaaaagaa
     7801 gccgataaat aaggtcacgc ccgtaccata atagccaaaa tccagctcac ctaaaaaaat
     7861 aaagccaaaa tagaccgccg tcaccgcaat cgataccgcc gtgaaaaact ccaataccgc
     7921 agaggataaa aacgccattt ttagtacgtc catcgtactg attcggaaat cttcggtgct
     7981 ttggtagatt tgttcagtct gcttttccgc ttgcccaaat aagcgaatgg tttctaaacc
     8041 ttttaattta tctaagaact gtccgctaat acgggaaaga atgccgatat tacgctgatt
     8101 tgcctccact gctttcattc cggctaaaat cataaataaa ggtaataatg gcattgttgc
     8161 aaataaaata gcccctgctg cccagttaat cgggaataca aaacagagga tcagtaatgg
     8221 cactatcagc gataaaaatt gctgtggcaa atagcgtgcg tagaaattat gtagattttc
     8281 cacctgctcg agcattaagg ttgcccagct gcccgccggt ttttgttgaa tgctcatcgg
     8341 cccgactgct tccattttgg ctagaatttg gcggcgaaga tgtaaacgca aggtttgccc
     8401 tgctttgaaa ccgacacgct cacgcagaaa aatgaagaat gctctggcag caaaacaaca
     8461 gaataagagc ataatttcaa ccagaaagct attcggtgac tgctttccga taagcatttt
     8521 gtctaacatt gtggcaatca gccacatctg tccaatcata cataaactac tgaaactccc
     8581 taacagtatg tttaagtgca tatatttttt gatgactttt tgctgacttt taagccattt
     8641 ttgtaattcg cgttggcgtt gtttttccat aattttgtca atttttttgt tttttcaacc
     8701 gcttgtaaat cgggtttgcc attctactat aaagcaaaaa ggttgttaat ggcgaaacat
     8761 taacaacctt tcattcagcc ttaactagaa tagctcgtca aattcaaagc gatacattaa
     8821 atctaccgct tggttaatac ttgagaccca ttgtaagtac aatcttggcg ttaaattata
     8881 gcgtaaggtt aattcggtaa gcggtgcaaa aatgcccacg ccatacttca ctctgaattt
     8941 aggggcaagg ctggcactta cttctacttt ggtgttatcg ccaatacctg cggtggtcac
     9001 gcttaaatct ttgagtccga aagtgctacc aacatcaccc accaatttac tgctttttga
     9061 taagctcata ctgagtaatg ccgctgcgac tgcattactg gagctggtat cgccactgct
     9121 ttccaatgaa cgaccagtta aaatatagga aagtgcttca ttttgcgaca ttgccggatc
     9181 agagaatact tttaccgttg gattgtctgc caaaccggtc actcttaccc cagctgtaat
     9241 ggtcggatct tccattgcct ccggattgcg tatagcttca atatctaagg ttggctgaga
     9301 cggtgagccg gcaaaactaa tatcaccttt acgaatcagc aagtcttgac cataagcggc
     9361 aaatcgtcct tcacgcaaat gaacttgccc gtaaagccct aaaccttgtt tgccttgcct
     9421 gacagacaat aagccattca cgttactgtt taaaccatag gctttgagtt tgacatcatt
     9481 accgatgtta atcttaatat ctgcattaat tgccattcca cctgaggttt gagccggaat
     9541 attacgttgg ctaaacggca ctttattttt cacagagccg tccataatca cctcatctcc
     9601 acttacactg accgcacttt caggtaactg ctcgacttca attcttgccc aagggatatc
     9661 tacatttcca cttaaggtta aaagagttgg cgttgccgtc acttgaatat ccgggctaac
     9721 tgccactttt gcgatattag gaatatccac ttggaaacgg ttagcttgag cgtgaacccg
     9781 tgttttccac gcatttagat ctcgccagtc tgcatcaccc tccaaacgca gttcgctttg
     9841 tttggtttgt aatttaccgc ttaaagtcga tgttgccccg tggaaattca ttgctaaatt
     9901 cccacctgta atatcaaacg gcattgtaac agagcgagct tttaactctg taacgtttag
     9961 atttccgtgc aataacggag aggttgcggt gccgctcacg gtaagacgag cattcacatt
    10021 gccatcaacc gaatcgcccc gttcaagtag cggtcgaatc agctttaagc ttaattggtc
    10081 aagatgaata ttgccggata acgcacgagc cttacttaaa tctttcatca ctaaatcagt
    10141 cgaaagtctg ccgttatttt cgacccttaa atcagattta agggttaaat tattgtcaga
    10201 cattttcaga tcaattttaa gcggtgaaac ggtgagtggg aaactttttc ccccctccat
    10261 tttttgaata aatttaatcg cattagaggt taaatccaga ttcacttgag gctgtttgtt
    10321 tttaaaccaa gctgcatcgc ctttggcatt cacaatccct gaaagctggc tgtttttctc
    10381 caaaaattgt tgcaataccg ctaaatcaaa gttacggatt tcaaaaggca ctttaccctc
    10441 aacaccggca ttaaatgcag tcgggaaaca aagattaatt ttccggttat gccaacaatg
    10501 tgcggaaata ttggcgttaa tggctttatt gtcatatttc acattcactg ctttatctgc
    10561 ttggaattga ccaaactcgg tggattgaat cgaaacttgg cttaattgcc cttcccaaat
    10621 ttctttcaaa cggtcaaatt ttccagaaat ttgcaaatca gcaccaaccg gattgccttt
    10681 tgcacttaat ttcaagctat gattttcttc atttcctttt agcaataaag tggcatttcc
    10741 cactttgatt tcattatagg caaattgacg taaccccaag gttaaatccc cttgaatggt
    10801 tttctcagtg gtaattttgc cttttgcggt taaattttgc agtttaaatt gctcatagcc
    10861 tacatttgag gccactaaat ctaaatcaat gttaggttct gaaaccttac ccgataattt
    10921 ggcattacca ttcacgctgg cttttaaatt cggcactaac ccttgcaaat taggggcttt
    10981 aatatccgca tagaaatcag atttttcgcc taacacgcct tttaaatcga tcttattttc
    11041 accatacacc agcgataagc ccggaacatc taaaagttgt tgatgatcgg cttttaaatt
    11101 accgcttaag tgtagatttt tctgtaataa cgaaccttta atatcaatat tcgagacgtc
    11161 cgctttccat ccgctagctg aattgccccg tcctgcataa ccttcggaag ataaagaacc
    11221 ggacaatacc gccggccagt ctgccagtag ggatttagtg ttaatatgct gaagtttcaa
    11281 atttgaacgc cacgcaacgc cctcgctcca atccactaaa ccgtcgatct tcgccgtacc
    11341 ttccagcgta ttgagactta aatcttgtat attaaaatgg gttaattccc ctttgccagt
    11401 taaactagcc gagcttgcag gcacgcccat tcctttggca ctgactttag cctccaaagc
    11461 gtaatccagg agattgccat taagcctcat ggcgatattc tctaaactca gcacatcacc
    11521 attttgctga ggagcaaacg gataacgcat tgactcactc ttcagcgata aatcaaaggg
    11581 agttttcgcc tgagccagtg caacattgcc ctccaacgag gcgctaattg caccagccgt
    11641 cttcactttt aggctggttt tgtcatatag ctcaccggaa agagaggctt caatttgatt
    11701 gaacggcaat tttactttcg ccgtttttgc ctcgtgacca cttagcgtcc aacttagcgg
    11761 ataattatct tgtaacgata aagaaccgct tgcggttaga tccccttggt cgcttttgaa
    11821 atctaaactt tttaggttga tatgctgatc ttttgcttga gcctgaagct gtaaagaggc
    11881 aacatttaac aatgagatcg gctcaatcaa attccctttt tcatctttag ctttttgagc
    11941 aatcgctaca ttcgcaatat caatctgttc aatattgaga tcaagaggca aactaaagcg
    12001 aatattttta tccaataacg gctgtgctaa ttgtgcttta atcgctgccc aatccacttt
    12061 attatccacc tgttcggcct gtgcaacagt ttttctctgt aattggtgaa agccctctac
    12121 gactttttca ctggtttttt ccaccgcctc ttgaatcact ttatcagcaa tctcttgtgg
    12181 atttgtcgct gataacgcct ctgtttttgc atttttttgt gcaaaatcga ccgcttgttc
    12241 agtatcagtt tcgtctaact ttaaggctaa aagcagattt agcccctcta actgtgtcgg
    12301 gtgaacggta atagcccgcc ctttaccaga aagtgcagtg tggaaatgat taagatgaat
    12361 atccatatca tcaactttga ccttgatatt ctctaaattg atattttgag ctgaaatccc
    12421 caatggcaaa tttaattcgg tgaaaggttc ggtctcttct tttggctgag aagggggtaa
    12481 tttagtcgta tcaatcaaca cctctgtatc tttaaggctg agattattca aacacatttc
    12541 atagtggagc aagcagctaa aaccaatatg cagatctgct tgcccaaccg ttacattcac
    12601 accgtccatc acaaatcggg catcagaaag ctgtaaccca tcttgcaggc taccttgcac
    12661 ttgcccaagt tgtaatggct ctacgaattt atctacccag cttaacactt tacgttgccc
    12721 gtaacctgtg catagaaaga gaatggcgag taaaagtaga ataaacgtca gacaaaagcc
    12781 gtgcttcaaa aaccgccact ttgatttttt aggctgtgtt gcttgtggct cttgtgctga
    12841 gtccgtttga tttgtggctg tttgtttatt ttcgtcagtc ataatcgtgc atatttatga
    12901 ggtgataaaa agtaaacaag cggtatgcga aattttactg aaattcgacc gcttgttttc
    12961 atttacagat cggaacctaa accaatataa atttgtattc ctttggaatt atctggtgat
    13021 tttaccggcg tggcgacatc aaatttaatc gccccgatag gtgatgccca acgcacccct
    13081 aagcccgtac cgtagtagag atcctgagtt ttgaatttat tcgatgccaa gcctgcatca
    13141 taaaaaattg caccccacca attagggtaa acctgatact gatattctac tgtgcctgtg
    13201 gctaaatgag agcctccgac acgtttgcca ttactcggat cacgggggga aatatcctta
    13261 tagccaaagc cccgcacact catatcgcca ccggcaaaat agcgtaacgc cggtggaata
    13321 cgttcaaatt gattggcttt caaatacccc aactccgccc ttaaatagaa ccgatggtta
    13381 tcaaagtaag ttctcgccca agatgtcgag gctttataac tataaaagtt tacatctgaa
    13441 cctactgcac gactccccca gtttaccgtt aatttttgtg tatcccccca taatgggtaa
    13501 ggcttgccat ctgttctggt tcgattcaat gatgccgttg ggtaaactaa taaggtttta
    13561 aatcgatcat tagcttgagt aaaagaatcg taacgggctt ttaaaccaac agaaaatgac
    13621 caaccggttt cgtgagtcca gaaacgttgt aagccaaggg tggcagcggt tgattttgta
    13681 tcgttttgat cttcgttttc taaagtgccg gataattggt aataataatg tagcggatct
    13741 tctctaagtg gaatattata accaaattca aatgtctgct ccggcttaga aacgtacgtt
    13801 cggctttcaa tactatgacc tcggctattt aaccaaggtt tcttccagtt aagctgtaac
    13861 cttgggccaa cttcagtttc gtagcctatc ccaatttcca cttcattgcg ttttttaggc
    13921 tgaaataaca catttaaatc cactaattta tcctgttcat ttaaatgtgg ctcaaccatc
    13981 actgatgaaa accaattact ggaaaggtaa tcttgggtta aaatcgaaag atcgttagct
    14041 aaatatggct caccggaatg aattttaaga atattttgca aatattcttc tttgatttgg
    14101 ctatcaacaa accggatttg tccataatgg tatcgttttc cgctatcata gcctaaacgc
    14161 caatccgctt tatgatcggt cggataaact tccaaacggt gataaagcca atgaccgtca
    14221 aaataccctt tactaaaagc ggtactttca atatcacctt taaaattatc ataggtttta
    14281 tggtttaaac gactaccttg ttttggggct ttactgacta gatgagcgaa atcttcgtcc
    14341 gtttttgcat cgccttgcaa aacaatatcg gtttcatcaa tttttaccgt ctctttatct
    14401 agctcaacat ttaatactaa gagcggtttc tttccctctt gaggtagctg cacaaattgg
    14461 tatcggctat tataatagcc caaggctctc agagctttat ctactgtggt ttgcaccaaa
    14521 tattgataac gctctgaacc atctgcatat tcattggcaa tttgatctaa atatagctcc
    14581 acatttttct tggcatcttt accctgaatg cctttgactt ctaaagtgac taatggctct
    14641 atttctggct catcatcact atcttccgct aatttttctt gcagattttc aactcgctca
    14701 cgatctacgt cagacagcct ttgctcttga tttgcttgtg ccattgaagc aaaagccact
    14761 aaacagcaga atgttaaaaa atgtagtttt gtattcatta gtaacctttt tattcttttg
    14821 gaatgccacg caaaagcgga atactgtcat aaggcggaac agaacttaac gctggctctt
    14881 gctcaaagag cataatgaat ggtttgtcag gcactgtttt ttcattacgc cacaagtttc
    14941 caacactcac caattcaata tcttgaggta gttgtgctaa attttgcttc agtacactaa
    15001 tcgtattctt gcgagggtgt cctatcacca ccgctacccc gttttttcgt gcgtgattaa
    15061 ttgcatgtgc aaactgtttt tgtacatcag caagcagatc gctatcatct aaaaaaatgt
    15121 ctctctccag cgtattaata ccaaattgac gagccgtttt agccgccaca ctactgccaa
    15181 tagttttact gtctaaaaag aaaagcgtat tttcttttaa cactttcaac aaatgttgca
    15241 ttgtagcaga gtctgaagtt gctccactgc ccatatggtt atttaaacca atagcatatg
    15301 gtacttgacc acgagaagta tgaataagtt tccgaatttt ctcttcgtta tcaccaatat
    15361 gaataccccc ctcttcaatt ggctgtttcg atttaggttc cattggcaaa tgaatcaaaa
    15421 tatcccgttt ttggttataa gcgtcctttg cccgttctgt tgcatagggt gcaacaggaa
    15481 taatcgccac gcttaccgcc ttgggtaagg ctaagatttc tcgatcttct tttattcgat
    15541 agcccacatc atcaattaca atcgccattt gagctgcttg tacaagcggt gtaaaaagca
    15601 caaacatttg caaaaaaatg ttaaaattca accgcttgtt aatttgttta aacatctatt
    15661 tcaccaaatt tagaggatta atcggcgtgc ctttacgggt aacaccaaaa tagagtgcag
    15721 aacggctttg tcccccactg ttaccaacct tcgcaataac ctgtccgcct tgcactcggc
    15781 tacccttgct gactgaaata gattggttat agccataaag ggaaatatca ccattgccgt
    15841 ggtcaatcgc aaccatattg ccataccctg caagccattg agccattatg actcgcccgc
    15901 tcgccattgc tctaaccgaa gtaccggcag aagcaccaat cactacaccg tgccaattat
    15961 taccaaaagg agtgataata ttgccagaca ccggcatcgt atatcgacca gccttgatcg
    16021 gtgttctctc aacattctca gttgttttgg actgaccgga ttttgcctgt ttttcccgct
    16081 gttgttcacg tttttctgct tgctgagcct ctctttgcgc ttgttctaat tgttgtttta
    16141 gtgcctgttc attgctgcgt aaattctcta atcggctttg atcctgctca agcgttttat
    16201 cgattaaacg taaggtactt tcacgctggc tttgtacttt ctttaaatct ttctcttgtt
    16261 tcttttgttc attaagttgc gtttgatgat ctttttgctg gcctttcaat tcatcacgtc
    16321 tggctttaag atcagcttgg gttttacgaa tttcaacgat cgcatcaatg cgaatattat
    16381 tcatatgctg ataataagcg gtcattcttt ccgcattttt tgcctcttca gacattaaac
    16441 gctctaagac cgaagggttc accccagaac ggtaggcaga gtccaactgc tcttttaatt
    16501 tttctttctg ctgtttttcc aacttttcta aacgggcaat ttcagtctca gtcgctttaa
    16561 tggtctggcg aacttccgtt aaagacattt cggttttttg taatttatcc aacaccttcc
    16621 ccatttctac ttcttggttt tttagggtag attgcaacgc attacgtttt tgcttttgtt
    16681 cattaatttt tgaggtttgc tgttggattt tttgctgaat ctgattaaga ttatttgcaa
    16741 aagaaaaagg ggtagaaaag cttattaccc cactgaaaat agcagtccaa cagtaagaat
    16801 ttattcgttt caccagcata cctttgtttc ttcaaaatat tattaaggtt atattctaat
    16861 cggttatttg cttaagatca gctttttagt gaaagaattt aactatctat ttcaaaatat
    16921 aaagaatttt gctataaata gcacaatttc aaatccatta attttactca aagctaagga
    16981 gtcattatgc aattagtatt tatccgtcac ggtttcagtg aatggaatgc taaaaactta
    17041 tttaccggtt ggcgtgatgt taatttaaca gaacgtggga ttgaagaagc aaaatctgcc
    17101 ggtcaaaaat taaaagcagc cggttttgaa tttgatattg cgtttacttc ggttttaacc
    17161 cgtgcgatca aaacttgtaa catcgtatta gaagaatcag accaattatg gatccctcaa
    17221 gtgaaaaact ggcgtttaaa cgagcgtcac tacggtgcat tacaaggttt agacaaaaaa
    17281 gaaactgctg aaaaatatgg tgatgaacag gttcacatct ggcgtcgttc ttatgacatc
    17341 tctccaccgg atttagaccc acaggatcca aactcagcac ataacgaccg ccgttatgct
    17401 aacttaccaa aagatttaat tccaaatgca gaaaacctca aaatcaccct tgagcgtgtg
    17461 ttaccattct gggaagatca tattgcacct gcattaattt caggtaaacg cgtattagtt
    17521 actgctcacg gtaactcatt acgtgcatta gcaaaacaca tcgaaggcat ttctgatgca
    17581 gacattatgg acttagaaat cccaaccggt cagccattag tgtatgaact agatgacaac
    17641 ttaaaagtaa tcagcaaacg ttacttataa ttggtaaatt tccgataaaa aataaccgct
    17701 tgtagtgcaa cacattacaa gcggttattt tttgttaatt ttttgcaaat ggcaaagaac
    17761 aaaaataaag gcgaatcata cattcgcctt attgttttaa agtagattag aaatctaaca
    17821 actctttctc ttttgctgct aatacttcat caactttttt aatgaaggta tcggtgattt
    17881 tttgaatatc ttcttcagct ttgtgttgtt cgttttcgct gatttcttta tcttttaata
    17941 acgctttgat ttggtcatta gcatcacgac gaacattacg aattgccact ttgccttgct
    18001 caccttctgc tttaacgatt ttgattaaat cacgacgacg ttcttccgtt aatggtggaa
    18061 gcggaacacg aatggttgtg ccggcagaag atgggtttaa acctaaatct gaggttaaaa
    18121 ttgctttttc taccgcagag attaaagatt tatcaaatac gtttaccgct aaagtacggg
    18181 catcttcagc taccacgttc gctacttgac gtaatggagt cgcagagccg taatattcca
    18241 cttgaatacc gtctaataaa gaaggttggg cacgacctgt acgaattttc gcaatatgcc
    18301 cttttaaagc ctcaaggctt ttttccatac gatcttgggt atctttcttg atttcgttaa
    18361 tcattgattt tttatccttt tatttagtaa ctgagagtta aacagagaaa attaattatt
    18421 cagaaatggt tgtaccttct tcagtaccga ataccacatt gcgtaacgcc cccggtttgc
    18481 ccatattaaa gacacgaatt ggcattccgt ggtcacgagc aagcgtgaat gctgctaagt
    18541 ccattacttg taattcttgc tcaataactt gcgcatacgt taatttatta tatagttttg
    18601 catctgcgta ttttgctggg tctttatcat aaacgccatc aactttagtt gcttttaata
    18661 ccacatcagc ttcaatttca ataccacgca agcaagcagc tgaatcggtg gtaaagaacg
    18721 ggctacctgt accggcagag aaaatcacca cacgtttttc acgcaacatt ttaattgctt
    18781 cagaccagtt gtaagtgtcg caaatcccat ttaattggaa cgcagacatt aatttagcat
    18841 ttacctctgc acggtgtaag gcatcacgca tagccaaacc gttcattacg gtggcaagca
    18901 tacccatatg gtcgcccact acacggttca ttcctgcttt ggctaatttt gcaccacgga
    18961 ataagttacc gccgccaagt accacaccaa cctcaacgcc ttcagcaagt aattctttaa
    19021 tctctaacgc catacgatcc aaaatagacg ggtcgatacc aaaaccttca ctgccttgta
    19081 gggcttcacc acttaatttg agaagaattc gcttataaat aggtttgctc attttaattg
    19141 cctcttaact gtatggacta aacacaaaat tacgttatta tagaacaatc caatataacg
    19201 ataaactaaa aatattcact acaatgaaat ccaaacaact tttcggcatt cttgtcgcta
    19261 tttcgctggc aactgctgct agctgtttaa tgctaatcgg atcaggcttt tttccaaatc
    19321 cgagtatctt tgaagtcatt ttgggcgtat tcctcatcat cgctcttgcc agctctaaat
    19381 ggacttatta tttactctta ctgccaatca gcatcgctta tgcaatttat accccggttg
    19441 ggataaaatt cggtaaaccg acttacgagt atatcgcctc tatttttgct accgatatga
    19501 tggaaagcaa agagtttatt gctcaactgc cgattttaaa tattgcggcg ggcattgcga
    19561 ttgtactagc ggtcattttt tatcgaaaaa ttaccaatcg ctatcaaatc cgttttttac
    19621 gcaataaaac attcgtgatt tcggcttttg tattaatgct gttttccctt gcaccgttta
    19681 aattttttca ggagttctac aattcgagcc taaaggtaaa gaaagaattg gaagtgctga
    19741 acaatttaac gctagattca caatggggac aatcaaccct cagctcaact tcaaactacg
    19801 atgattatgt actgattatg ggcgaaagtg cgagaaagga ttatcaccac gcctatggct
    19861 atccggctgc caatacgcct tttatgagtt cggcaaaagg aaccttgatt gacggtttaa
    19921 cctccggtgg cacaaacact atcgcctcat tacgcttaat gctcactaag cccgatacgg
    19981 aaaaatggga agcaaattat gggttaacct taattgattt aatcaaatct accggtatca
    20041 agacctattg gatctcaaat caaggctatt tgggcaatta cgatacgccg atttcctcta
    20101 tcggtaataa aagtgatgtc aaaattttca ctaaagccgg cgattcatta aataccaatg
    20161 tgagcgattt tgaattgtta ccgcattttg ctaaagtgat tgaagaacct gctcaaggga
    20221 agcgtttcat ctttctgcat ttatatggct cgcatcctat tacctgcgat cgtctcaccg
    20281 attacccgaa aatgtttgac gatgacaaac tgccaaaacg ctactttaat gtaaattgct
    20341 acgtgtcatc tatcaagaaa accgatgaat tgctgaaacg ggtatatgat ttgctagtga
    20401 aaaaccaaga aaatcaccag cgttcattct caatggttta tttgtctgat cacggtttat
    20461 cgcacgaggt ttcaaaagag aacattgtta tccacaatgg aaaggataga agtaaattac
    20521 actacgatat tccgctgttt aaaatttcaa gcgatgatac ccaacgtaat gagtataaag
    20581 cctttaaatc cggcttaaac ttcactaatg gtattgcgaa ttggattggt atcgaaaatc
    20641 cgttattaga taaagaaatt aatcttttta acgaacaaaa cgataaaagt gattatgggc
    20701 taaagaaaat cattgaaacg cagtctactg tagaagatcc agctgttcct attcctgaaa
    20761 gctacttata taaagcgact aattagcaaa agtaagtaac aaaaaagcag gctttaagcc
    20821 tgctttttta gtttttagta tcagaaaaat taagcgttac cgccagtgat tttcgctact
    20881 tctgctgcga aatcttcttc tactttctca ataccttcac ctacttctaa acgaataaag
    20941 ttagaaactg aagtatttac tgattttaaa tattgaccta cagattgaga tggatccatt
    21001 acaaacggct gaccggttaa tgaaacttca ccggtgaatt tagccatacg gccttcaacc
    21061 attttttctg cgatttcttt tggtttacca gaattcatcg caatttcaat ttgaatttca
    21121 cgttcttttg ctactacatc agcagaaaca tcttctgggt ttacaaattc aggtttgctt
    21181 gcagcaacgt gcattgctac tttcttaagc tcatcttcag agcctgcacc tgctactaat
    21241 acaccaattt tcgcaccgtg taagtattga gcaataactt cgccttctaa gtattgaaca
    21301 cggcgaatgt tcatattctc accgattttc gcaactaatg ccgcacgttt ttcttcaaat
    21361 tgtgcttgta atgcttcgat agttgtacct ttatttgcaa ctgcaaaatc agcaacttcg
    21421 ttcgctaatt ctaagaaacc ggcatctttt gcaacgaagt cagtttcaca gttcatttca
    21481 actaatacgc caaaacctga agcgacacga gcaaggatta caccttcagc tgcaacacga
    21541 cctgcttttt tagccgcttt cgcttgaccg gatttacgca tattatcgat tgctaattca
    21601 atatcaccgt tcgcttcaac taatgctttt ttacattcca tcatacctgc gccggtacgt
    21661 tcacgaagtt ctttaactaa tgatgctgtg atttcagcca ttttaaattc cccttatttg
    21721 atatggtaaa acaggctctc acctgcctag aaatttcatt gtttagggca ggttaaaacc
    21781 tgccccctca actgaattat tccgcagaag cttcttctgc tacttcaaca gtttcaacac
    21841 cgcgaccttc tttgattgca gcaactgctg aatctaagta aagttggata gcacgtgttg
    21901 catcatcgtt accgggaatg atgtagttaa cgccatctgg agttgagtta gtatcaacaa
    21961 tcgcaaatac cggaatacct aagttgtttg cttctttaat tgcgatatgt tcgtgatcag
    22021 caccgataac aaaaattgca tccggtaaac cgttcatatc tttgataccg cctaagctta
    22081 actctaattt ttctaactca cgagtacgca ttaacgcttc ttttttagtg attttgtcaa
    22141 aagtaccgtc ttgagtttga gtttctaaat cttttaagcg tttgattgat tgacgaactg
    22201 ttttccagtt agtcaacata ccacctaacc aacggtggtt tacgtagaat tgttggctct
    22261 ctactgccgc tgctttaaca gcatcacttg ctgcacgttt tgtaccaaca aataatactt
    22321 taccattgtt actagcaatg cgagttaatt ccgctaatgc ttcgttgaat aaaggtaaag
    22381 ttttttctaa gttgattacg tgaacaccat tacgagcacc aaagatgaat ggtttcattt
    22441 ttgggttcca gtaacgtgtt tggtgaccat agtgaacgcc tgcgttaagc atatcgcgca
    22501 tagaaacttg tgccataaga attttccttc aaagttgttt gttttgccac ttatgaacgg
    22561 caaaacgggg tttagcctcc acatatccta gaaaatcaat ctgttaaaga caccccaatt
    22621 cctagcttgt gatatgtgtg tgttttagtg ttaaaaaata aatgtgtacg ttttgcctga
    22681 atagacaaaa acggggcgta ttatgccaaa aaacaaacaa tttgccaatt attttattgc
    22741 tctcttcgca ttactaaatc aagctcaaaa ataaaaaccg ttctcattat tgactaaaaa
    22801 aatggggagg tttagaatgt ctttcattct gttatgaaaa cctgtctgaa ggagactatc
    22861 atgaaaaaaa cactttctgc acttgctatt gctgcagcca cttttacatc aacttcaacg
    22921 cttgctgcaa atgaagtgaa tgtttattct taccgccagc cttacttaat tgagccaatg
    22981 ctaaaaaact ttgaaaaaga tactggcatt aaagtaaaca tcatcttcgc tgataaaggg
    23041 ttggttgatc gtgtaaaaca agaaggtgaa ttaagtcctg ctgatgtgct tttaacagta
    23101 gatatttcac gcgtaatgga aattgttaac gctgatcttg cacaaaaaat tgactctaaa
    23161 gtgttagaaa aaaatattcc tgcacaattc cgtgactcta acgaccaatg gtttggttta
    23221 accacacgtg ctcgtgttat ctacacgtca aaagatcgtg taggtaaatt accagcaggc
    23281 tttgactacc tagatttagc taaaccagag tataaaggta aagtctgcgt gcgttcaggc
    23341 aaaaactctt acaatgtctc actattcgcg gcgatgatcg aacattacgg cattgaaaaa
    23401 accaaagcat tcttagaagg cttaaaagcg aacttagcac gaaaaccaca aggtggtgac
    23461 cgtgatcaag taaaagcaat taaagaaggc atctgtgatt actctatcgg taacagctac
    23521 tactacggca aaatgttaga tgatgagaaa caaaaatcgt gggctgaagc agcgattatt
    23581 aatttcccga gtggtgaaca cggcacgcac aaaaacatca gcggcgtagt tattgcaaaa
    23641 cactcaccaa ataaagcaaa cgcggtgaaa ttaattgaat acctaagtgg cgaaaaagca
    23701 caaggtttat atgctgaact taaccacgaa tatccagtaa aagaaggcat cgagccatca
    23761 gcaatcgtaa aaggttgggg tacgttcaaa tcagatacca tcaaacttga agatatcgca
    23821 aaaaattacg aagccgcatt aaaattagtt gatgaagtga aatttgacga tttctctgag
    23881 aaaaaataat caaccgataa gcggtcattt ttccacaaaa atttgcaaaa ttttgttaaa
    23941 atacaaccgc ttgcaaaagg acgcattttg cgtcctttta tttttttatg aaataacagg
    24001 cttaaagaaa aatgcgatca caattttgct ggaaaagtat tgctgcccta acaactttta
    24061 tcgttttgct tcctcttatt gcaatttgct atcacgcatt gcaaggggat aatgaaaacc
    24121 ttgctcacct ttggcaaaca atgttgccaa tctatcttaa aaactcaact ttattagtat
    24181 taggaaccgt tttccttgca ctgatttttg ctctgcctac cgcttggatt gtggcaaatt
    24241 accgtttttg ggggcaaaga accttacaat ggttactttg tttgcctctc gctatgcctg
    24301 cttatttaat tgcctattta tataccgatt tattagatta ctcaggtgct tttcaaggct
    24361 ggctgcgtag tatttttggc tggactcatc cgcaagatta ctggtttcct caaattcgta
    24421 cattgtatgg tgcttgcttt gtgcttgcgt tagtactata tccatacatt tttttattag
    24481 tgcgagttgc cttattagaa caatctgaaa acttaacaca cagtgcaaaa atgctcgggg
    24541 cttcgccctc tcagttgttc cgtcgaatta cgctgccatt aattcgacct tctgttgccg
    24601 taggcattgc attagtggca atggaaacgc ttggcgattt cggtactgtg gcctattttg
    24661 ccgtgccaac acttactacg gcaatttatg attcgtggct aggctatcac gatcttggta
    24721 ctgccagcca gattgcgatc tttatgttgc taatgatttt cctgttttta agtttagagc
    24781 aatatagtcg ccgtaaacaa aaaagctatc aacgtggcta tgaaaagaaa tcagccttta
    24841 aatttttaac tggttggaaa ttagcattag cccttgcttg gtgctggggt ttattggcgt
    24901 tagccttttt cattccgttt ggcaggttgc tctattgggc atacgactat ttcgagcaag
    24961 cgtggaattt agactttgtc caattttcgc ttaatagttt gaaagtatcg atcatcgcct
    25021 ccgtgataac ggtactgatt gctctacttc tgcatttttc taaacgtttg aatacccgca
    25081 aaattttagt caaaaatgac cgcttgtcat tacaacttgc ctcactgggc tatgcggttc
    25141 ctggcacagt attagcgatc ggtttacttt cgcctttaac atttgccgat catcaactcc
    25201 atgcttggct aaaaagcctt gaattatcac ccgttggatt gattttttca ggctcactct
    25261 ttgcattagt attagcttat accatccgat ttttagcaat ggcaattggc agccttgaaa
    25321 cttcgctaaa taaaatctct ccatcgctgg atatggcgag ccaaacgctt ggtaaaaatg
    25381 gctcagcaat gttagtaaaa attcatatcc ctttaattta taaaggtatt ttaactgcag
    25441 gattaatggt ctttattgag agtatgaaag agctaaatgc ttctctgcta cttcgcccat
    25501 ttaattttga tacactcgca acctatgtgt ttacctttac atcagacgag cagcttgagc
    25561 gtgctgctct gcctgccatc gtattggtgt tagtcggttt actgcctgtt atttggctta
    25621 ccagatccct tatttcgcaa tctgaaaaag aaagataaag gaattttatg tctgttttaa
    25681 ctattaatca tctcaacctc aatttcggca gtacaaaggt tttacaaaat ctccatcttg
    25741 ctgtgaatca aaatgaaatt atctgtttgc taggtgcaag tggctgtggg aaaactacac
    25801 tgctcaaagc cattgcaggt ttattgccaa ttgagcaggg agatattcat ttagcgggtg
    25861 aatgcctaaa aagtaaagcc gtggaagagc gacaaatcgg gctgattttc caagattacg
    25921 ctctttttcc acacttgacg gtagccgata atattcaatt tggcttatcc aaattgccaa
    25981 aagtacaaca aaaagcaatt accgaaaaaa tgctttcagt tgtgcattta caaggctttg
    26041 aaagccgttt tccacacgaa ctttcgggcg ggcaacaaca gcgtgtcgct attgcccgag
    26101 cattagcctg ccagccagaa ttgctactgt tagatgagcc gttttccaat attgacagcc
    26161 aaactcgcta tgcaatgatt caagagatca agcaaatctt aaaaagccaa aatgtgcccg
    26221 ctatttttgt aacccatagc aaagaagaag cctttgcatt tgccgataaa attgccgtga
    26281 tggatcaagg gaaaattatt caatttggta agccaaacga gctataccat tctccagtta
    26341 atccatttgt ggcagatttt ttaggtggca caaactatct taactgtaat gtaacggaag
    26401 acaacgtgtt atgcagccct attggcgagt tccgactctt ccctgaaatg aacgttcaga
    26461 aagggcgtta ccgttggctt cttcgcccag aacagattat cttaaaaccc aatgagctag
    26521 gtttaggtaa agtggtaggc aaactgtttt taggcctata ctaccgctat caggttgaaa
    26581 ttaaaggcat tgagatagta acttaccaat ctcaagagct gaaaattggc gaacaagtgg
    26641 aagtgggctt ccaagtaaga gagtttatcc tattttaagg agaatatatg aaaattgaag
    26701 tttggtcaga ttatgcctgc ccgttttgtt acatcggcaa acgccactta gagcaagccc
    26761 tagcccaatt tgaaggcgag gttgaagtgg ttttccgagc ctttgagctt gatccgcacg
    26821 ctaacggcga accggagggc gatattcagc aacgcctaat gcggaaatat caaaaaaccg
    26881 cagaacaagc tgatgaaatg attcgttatg tggaacaagc cggcaaacaa gctgggctag
    26941 atttgcgtta ccgcaccacc caatacactc gcacctttga agctcatcgc ttggcgaaat
    27001 ttgcagaaag taaagactta ggcgaagcaa tggttgaacg tttattcaaa gcctatttta
    27061 ccgataacac tattctagct aaacgcacag agcttatcag tttagcctta gacattggct
    27121 tagagcgtga tgaaatcgct caactgctca caggtgatga tttcgggcac gaagtccgtg
    27181 aagatgagcg agtcgctcac aaatatggca ttcacagcgt accgtttttt gtgatcaatg
    27241 aaaaactcgg ggtatccggg gctcaaccgc cagaaatatt acttgacgct atcaaacagg
    27301 cattgcaaaa ataatccgaa aaaagaccgc ttgttaagct aaacaagcag tcttattctt
    27361 cttataattt acaatttatt ttttggtgat cgcacctaaa ataccacgca taatttgttt
    27421 ggtgacttgg ttgcgtaaat ttctgccaac tgaatgcgcc acttggctaa ccacttgttc
    27481 ggtaatcgat tgatcttttt tcttcttcga gccaaaaatg gaagagagaa taccgccaaa
    27541 gaagtcattg tttgcttctt ctgccgcagc ctgctctgct ttaattgctt ctgctttcgc
    27601 attaagtaat tcaaacgcag attcattatc gacatattgg ctgtagtgat gatagagatc
    27661 atcttgcttc actaaactat cacgttcgcc tgtggctaat ggagctaatt ggctttttgg
    27721 tggatagata taggcaattt ctacaggcgt tggcatacct ttttcatcta gcaccgaaac
    27781 caacgcttgc cccacaccaa gttgcgtgat ggcttctacc acatttacct ctttattcgc
    27841 tcggaaagtt tccgctgctg attttaccgc tttttgatca cgtggcgtga aagcacgcaa
    27901 agcgtgttgg atacgattgc caagctgccc taatactgaa tcaggtatgt ccaacggatt
    27961 ttgcgtaaca aaataaacac ccaccccttt tgagcgaatt aaacgaacca cctgttcaat
    28021 tttatccacc aatgccttag gggcatcgtc aaacaataaa tgagcttcat caaagaataa
    28081 aacaaacgtc ggtttttctg ggtcgccaac ttctggcaaa ttctcaaaga gctctgccat
    28141 aaaccaaagc aggaatgcac tgtacactct tggtgagtta attaattttt ctgcattcaa
    28201 catattgatt acaccacgcc cattacgggt ttggatccaa tcgtgcaaat ctaatgcagg
    28261 ctcaccaaat aaattatctg ccccttcatt ttcaagcctt aataacgcac gctgaatagc
    28321 tccaacgctt gctgcagaaa tatgcccata ttccagttgg aattctttcg cattatctgc
    28381 gacaaattta agcaacgcac gcaaatcttt taaatcaatt agcaataaac ctctgtcatc
    28441 tgccacacga aaaacgagat ttaacaagcc tccttgggta tcgtttaaat tgagtaaacg
    28501 agaaagtaac atcggtccca tttcggaaat agtgattcga agtgggatac ctgatgtacc
    28561 aaatacgtcc caaaacgaaa ctggatagcc ctttaagtaa tcttctcccc ctaaattaaa
    28621 ctgtaaaatg cgttctgcaa tttttcctga aaaattaccc gcttgtaccg agcccgaaag
    28681 atcacctttt acatctacta aaaatactgg cacgccatca tcactcaaag attctgccaa
    28741 tttgcgtaaa gtaaccgttt tacctgtacc cgtagcccca gcaattaaac cgtgccgatt
    28801 tgccattttg gcattaagcg aaataacttt gcctgtttct gttcgagcaa tttgatacaa
    28861 actcatcttt cattctctta aaggtaaatt aattttttgt tctcatcata taaaaattta
    28921 tcacttaatt acggctattc tttttcgttt ttgccaagaa cgttttttta cttcttgagc
    28981 ggttcacttt cggtaaagtc agtaaggatt ccttgtcgta ttgactcaca ggaatctgat
    29041 gcccaatata ctcttcaatt gcaggcaaat taaccgcata acgctcgcaa gcaaaactaa
    29101 ttgaatggcc gctctcccct gctcgccccg ttctaccaat acgatgaacg taatcttctt
    29161 tatcatcagg cagatcataa ttgaaaacgt gcgtgacatc tggaatatgc aatccacgag
    29221 ccgcaacatc tgttaccact aaaatatcca aattgccttt ggtgaaatta tccagcaaag
    29281 aaaggcgttt tttctgcgga atatcgccgg ttagcattcc aacccgatgt ccgtctgccg
    29341 ataaatactg ccagatctct tcgcatttat gcttggtgtt ggcaaaaatc atacaacgct
    29401 ccggccactc ttcctcaagc aatgtcagca acaatgccat tttgtcttca ttcgaggggt
    29461 aaaatagctc ttctttaata cgatgtccgg ttttttgtag tggctcaatt tcaatatatt
    29521 ccgcattatc catatcttca taggcaagtt cccgcacttt tgccgaaagc gttgccgaaa
    29581 agagcaaagt taaacgttct gaaggcttag gtgcttttcg cattaaataa cggatatctt
    29641 taataaaacc aagatcaaac attcgatctg cttcatctag cacaatcact tgaattttgt
    29701 ctaaattgat aatgccttgt tttacatagt caatcactcg acctgttgta ccgacaagaa
    29761 tatctacccc tttttcaatt tcttgaactt gtttgtcgta accatcacca ccgtatgcca
    29821 aagctagttt taaatcagaa tgttttaaaa acagctcggc atctgcccca atttgaaccg
    29881 ccaattcacg ggttggagca agaattaatg ctctcggctg attttttgac gtttcgattt
    29941 cgttattcaa taaatggtta aaagttgcaa tcaaaaaggc aagcgttttc cctgtacctg
    30001 tttgagcttg ccctgcaata tctttgcctt tcagcgtaat cggaaaggtt tttgcctgaa
    30061 tcggcgtaca aaattcaaag cctttgctat caagggcatt tagtacatta gcgtgaatcg
    30121 ggaagtcaat aaagcggtgt tcggtaagat gattagacat agtatttcct tgataattgt
    30181 gatgtgacaa gcggtctttt ttacacattt ttttgcaaaa tagcatattg ggcgtttttt
    30241 ctcaaattta accgcttttg agtttattcg gcaaaataga ttggaaaagc tctcggcttt
    30301 tcagcggttg tgaccctaaa tagggcttta gtgccattcg ggtaaaacgt ttcgctgctt
    30361 gttgagtact tttttctgaa aaatccaact gagcaaacgc taataaatca ctgcctaaaa
    30421 agctctggtt attctgtaat aatgaggcga taaagccctg attttcctga aattgatagg
    30481 tcattttcgg atcaactggc aaaccggttg ccgcacagtg cgtaaaatcc accccataac
    30541 ccaatgcttt taacgtatga aattcaaagg tgcgtaaaat cggctcaatc tgtttttgtg
    30601 tggcaagctc ggtaatgcaa cgtaaataat gctgaaataa ttcagggtaa gcggtctgat
    30661 tttccaatac tcttgcaagt acctcgttga tataaaaccc actataaagt gaaagcgttt
    30721 ccatcggcaa cgttaatgca gccggttctg ctttggttaa agttttcaga tcccctttac
    30781 ccgaccaacg caacaacaac ggcgtgaacg gctgtaaaac cgctttcaac ggcgagcgag
    30841 cccgccttgc acctttagca agtaaagtaa tccgcccgtg atgttcggta aaaaactcca
    30901 ctaataaact gttttcgctg tattcacgcc gatgaaggac aaaggctcgt tgccattggt
    30961 ctattgtcat aatttcttgt taattatgag gttgtaattt acttctgcca aatctccccc
    31021 tcccctcttt gctaaagagg ggagatcttg cagagtatat tttaaagtca ttttcactat
    31081 ttaacctttt atatagatag gaagatgaat ttaaaatttc tggagaacta gtccccctct
    31141 ttagaaaaga ggggttaggg gagatttgat tacgatatta atgctataac gggagccaac
    31201 tactcgtcca tataccctaa acttctcaat gctctttcat catccgccca accagctttt
    31261 actttcaccc aaagctcaag gtgcacttgg ttgtcaaata ggcgttccat atcttttcga
    31321 gcttcaatgc cgatggtttt gattttttgc ccccccgcac caataaccat ttttttctga
    31381 ccatctcgtt ctactaaaat caagccattg atttcataca ccccacgctc attcatttta
    31441 aattgttcga tttctaccgt taccgaataa ggtaactctt cacctgtaaa acgcattagt
    31501 ttttcacgaa taatttctga tgccataaag cgttgagagc ggtctgtcac atactcttca
    31561 ggaaagtggt gctcaccttg acgcagtgat tgacggacaa atttctgtaa aatatgcaca
    31621 tttttacccc gttgtgccga aattggcaga atttcagcaa aatcaaattt ttgtgaaagc
    31681 tcggtaatat gcggcagaag ttcttctttt tccttgatgt tatcaatttt attgattgcc
    31741 aataaaacag gggcttttgc cgaacgcaat ttgtttaaca ccatttcatc atcttctgtc
    31801 cattttgtgc cttccacaac aaagatgatg agatctacat cgccaatagc agaacttgct
    31861 gcacggttca ttaaacgatt aatcgcacgt ttttcttcta tgtgtaagcc tggggtatct
    31921 acataaatcg cttggtattg atcttcagtg tgaataccaa caatacgatg gcgagtcgtt
    31981 tgagctttgc gtgaggtaat ggaaattttt tgtcctaaaa tcttatttaa taatgttgat
    32041 ttccctacat ttgggcgacc aacaatcgca ataaaaccac aataggtttt tgcaggagtt
    32101 tgaatttgtt ctgtcatttt gttttccttt cattctgtgg aatgaatttg atggtgcaag
    32161 cgtccacgct tgtaccattt aattaaatct ttagcacaag cgaggacgct tgcgctatct
    32221 tactttttcg ttttaaccac cgctaacgct ttttctgcgg cattttgttc ggcttttcgg
    32281 cggctggtgc caatgccgat aaacacttcc tcgacgttaa tcactttaca ggttacacgg
    32341 aaagtttggt tatgagcttc gcctttaata tctaatacat catattcagg caatggcaat
    32401 tttctacctt gtaaaaactc ttgcaggcgg gtttttggat ctttttgtga atcgcctgga
    32461 ctgatatctt taagcaaatc tgaataccaa tcttccacct tttccattgc ttttgccata
    32521 tcttgatcga gataaatggc cgcaataatc gcttcaacac agtccgataa aatcgattct
    32581 cggcgaaaac caccactttt tagctctcct ggacctaatt ttaggtagtc gcctaaatca
    32641 aatttgcgag caataatcgc tagagtttgc tcacgcacaa gagttgctct catacggcta
    32701 agctcacctt cattcgcttt tggaaatttg tcgtataata ctttaccaat agtaaaattt
    32761 aaaattgaat cgcctagaaa ttcaagacgc tcattgtttt tggttgccgc actgcggtgt
    32821 gttaaggcct gaatgaggta atcaaggtta tgaaattgat aacctaattt tttctgtaat
    32881 cgttctagtt gcatagttta tgtgaataaa ggggcgtatc tcatacgccc ctgttaagat
    32941 taattgattg aggtaaacat tctagaaaaa cgaagccccg ttgggaattc atttgctttt
    33001 ttatctaaac ttaaccaaat aaaagtggct ttaccaacga tgtttctctc aggcacaaag
    33061 ccccagaaac gactgtcttc actgttatca cggttatcac ccatcacaaa atagtatcct
    33121 tgtggcacaa cccattcacc ttctgcttgc ccttcttgtt ggtagaaata aggtgcatag
    33181 ttaaatgcca ttgggtgatt taaaatttga tgagttacat tacctttttc agtgcgttct
    33241 acctgcattt caccgtgata gaaaaactca ggatttggct tgccttcgct gtattcaaat
    33301 acctttgttt cgccattggc tttagtaacc gttaattgct gagttgcaaa atcatattta
    33361 attttatcac cacctactgc caccacacgc ttaatataat caacgtgtgg ttgtgccggt
    33421 gctttgaata caatcacatc gcctcgttct ggtttgcccg tttcaatcaa ggtgttttgc
    33481 cagataggat ctttaatgcc gtagctgaat ttatttacga ctaaaaaatc gcctacacgc
    33541 aaggttggct ccattgaacc acttggaatt tgaaacggtt caaataagaa cgagcgaagt
    33601 actgtaacaa agaataaaat gccgaaaagt gaggcaaaaa attcgcctac tgcggattta
    33661 ggttgaattt ccgctatttc ttcagcggtt aattctctgc ctaaacgttt ttgttgtaga
    33721 acaatcgcct gtttacgtct tgggtcagca ctgaatttgt aaaacgccca aaatccacca
    33781 caaataataa ccaaggcaac taaaataatg gaaatagtat ttggcagttg cattgaatca
    33841 agccattgcc aaataccata aataatacct agaaaaataa ttggcataaa ttgtgccact
    33901 gaatgctcct ttttattcaa aattgcaaag ttttctcaaa atatgaccgc ttgcaagcgg
    33961 tcgtatttta cacgaatatt gacaattaat ccttacccac atgaagaatt gccaaaaatg
    34021 cttcttgtgg cacttctacg ttaccgagag atttcattcg ttttttaccc tctttttgtt
    34081 tttgtagcag tttcttctta cgacttacat caccaccgta acattttgct aatacgtttt
    34141 tacgcaactg ttttaccgtt gagcgagcaa tgacgtggtt accaattgcc gcttgaatag
    34201 cgatgtcaaa ttgttggcgt ggaataagtt cacgcatttt ttccactaat tcacgtccac
    34261 gatacgccgc atttgcacgg tgaacgataa tcgccaacgc atcaacacgc tcgccgttaa
    34321 tcataatatc cacacgcacc atatcagccg tttggaaacg tttaaaaccg taatcgagtg
    34381 aagcataacc acgagaggtt gattttaagc ggtcgaagaa gtctaacact acttcgccca
    34441 ttgggatttc gtaagttaag gcgatttggt taccgtgata gaccatattg gtctgtacgc
    34501 cacgtttttc cacacaaagg gtaatcacgt tgcctaggaa ttcttgtggc acaagcatat
    34561 tacattctgc aatcggctca cgaatttctg caatattgtt gattgctggg agtttcgatg
    34621 ggctatccac atagatcgtt tcgccactgg tttgcaccac ttcgtaaact acggtcggag
    34681 cggtagtaat caaatcaaga tcgtattcac gctctaaacg ctcttgaatg atctccatat
    34741 gcaacaagcc taagaagcca caccggaagc caaagcccaa cgcacttgaa gtttctggct
    34801 catagaacaa tgacgcatca tttaggctta atttgcctaa agcatcacgg aaggcttcgt
    34861 agtcgtctga actaatcgga aataaccctg cataaacctg tggttttact ttcttaaagc
    34921 ccggtaatac ttcagttgct gggttgtggt ggtgggttaa tgtatcgccc actggtgccc
    34981 ctaaaatatc tttaatcgca caaactaccc aacctacttc acctgttttt agctcggtag
    35041 tatccacttg ttttggcgtg aaaataccaa ggcgatctac attgtaagac tgccctgtac
    35101 tcatcacttt aattttatcg ccttttttca gcacgccatt tttcacacgc actaaagaca
    35161 caacgcctaa atagttatcg aaccaagagt cgataattaa cgcttgtaat ggtgcttccg
    35221 catcgccttc tggtgcaggc attttcttca cgatttcttc taaaacatct tcaatgccta
    35281 aacctgtttt agctgaacag cgtactgcat caatggcatc aatgccgaca atatcttcaa
    35341 tctcttctgc cacacgctca ggctcggctg ctggtaaatc gattttattt aaaattggca
    35401 caacctctaa atctatctca attgcggtat aacaattcgc caacgtctgt gcttccaccc
    35461 cctgccctgc atcaaccact aataaagcac cttcacaagc agcaagcgag cgagaaactt
    35521 catatgagaa atccacgtgc cctggcgtat caataaagtt gagctgataa atttcgccat
    35581 ctttcgcttt atagtttaag gttacacttt gtgctttaat agtaattcca cgctcacgct
    35641 caagatccat tgaatctaat acttgagcct ccatttcacg atctgataag ccaccacaag
    35701 tttgaattaa gcggtctgat aaagtcgatt tgccgtgatc gatatgagcg ataatcgaaa
    35761 agttacgaat attttgcatt ttcatcgggc agttaatgtc ctgttttgtt atttgtttat
    35821 caaaaattaa tcggggaatt gtacttgaaa tacaaggttt tgcaaagaaa acaaatagca
    35881 ataagcggtt attttttcct aaaaatgtgc aaatttcttg agaaaaatga ccgcttgcac
    35941 tcaccctaat tgcaaataat ttctctaaaa tataaaaaac attgagcaat cacaaatttt
    36001 tattatagaa tccataagtt tttttaattc attcaattat aggaaataat tatgcaattc
    36061 agagaaatta atccctcccc tcgtttatta atgtgccctg gcccaacaga tgctgaccca
    36121 cgtgttttgc gtgcaatgtc tgcacccatt ttagggcaat ttgaccctga gtttacctca
    36181 ttaatgaatg aaacaatgga aatggaacgc cgtttattcc aaaccaaaaa tgagcaaact
    36241 tatgtagtca actccacctc tcgtggtggc ttagaaactg ttctaaccgc agcgatttgc
    36301 ccaggcgata aagtgttaat tcctgcattt ggtcgttttg gttatttatt aaatgaaatt
    36361 ttagctcgta gcggtgcaga tattaccatt atcgaaagag agtggggaac cgtatttgaa
    36421 ccagaagaaa ttgaagcaga gttgaaaaaa ggcggctata aagcagttgc tattattcac
    36481 ggcgaaacct caacctctat gatgcaaccg ttagaagaga tcggcaaaat ctgtaaacaa
    36541 tatgatgcaa tgttaattgt agataccgtt gcaaccttag gcggtgtaga tattcgtgtg
    36601 gatgaatggg gcattgatgc ttgtatcggc ggtacacaaa aatgtatttc cgccccatca
    36661 ggcacagctt taatcactta taacaaacgg attgaagaga ttattgcaaa acgtaaacgt
    36721 gtggaaaaag gcattcgtgc agagtctgat gtagatggca cattaccgcc aattccaagt
    36781 aactacttag acttggcaca gctacaagat tactggagcc cacgccgttt aaatcaccac
    36841 acagaaacca cttctgcaca atatggtgta cacgaagcgt tacgcattat tttacaagaa
    36901 ggcttagaag ctcgttttgc tcgccatatt ttaaacgaca aagcattatg tgcaggttta
    36961 gaagcaatgg gtttgaaaat ttttggcgat cgcaaacata aagcccccgt tgtaacggca
    37021 ttccacattc cagaaggtat gagtggccca gcattgcgta gtgatatttt aaaccacttc
    37081 ggtattgaat tagccacctc attcggcccc ttagatggaa aagtaatccg tatcggtaat
    37141 atgggtttct caagccaaaa acgtaacgta cttttaacct taggtgcgtt agaagccgta
    37201 cttttaagcc ataaagtaaa agtgccttca ggcgatgcgg ttgcagctgc cttggcagtc
    37261 tataaagagg ctgataaata accaccttcc taaatttaca atacaactca ctaataatta
    37321 atactgtgga tatttatcca cagtaaaacc acagaaactc aaaatatttt cacaaattta
    37381 atcttttcct atttcattta atcaaattaa atattatgac acggagcatt tttttaagaa
    37441 tgaaaagtaa aagttttaat gaggaaattt tgatatgcca aactttagtt tcacccatct
    37501 gtataaaaaa cacacaaaag agctccctac tgttaccttg cttagcgctc tgatagggct
    37561 tagccaagtc tcttatgcca atccacttcc tttaggcaac accactggca ccaataacct
    37621 tgctgtaggt gaaggtagtt ttgcgttagg cacaggttct caagcaatcg gcaagaatgc
    37681 cattgcaacc ggtggtaata tctcccccca ggaattcaat gtggagttag aaaacttccg
    37741 tgatttagtt aatcaaattg aagagttacg tactcaaatt gctgaacaag aaaaacaagg
    37801 ctctgttaat caacaattac aagatgcttt aaagagtcag attgctcaat atgacactat
    37861 tttagagcgt gtacgtgaaa aacaagcaca acaagcaata ttagccaata gtgttatatc
    37921 aatgcggaaa gaattagtta atagaagaaa tcagttaaca gaattggaaa ataattttaa
    37981 tactagtttt ttaacaggaa aaaataaaca agaagcatat caaaaatttt ctacagtaat
    38041 agaatcatta gactgggata aattatcaaa aactaatggg gtagatcaat tagcaacaga
    38101 cttaaaaact aaaattgaaa ctgatttccc taatattaat ttttcaaatg aaaaatataa
    38161 agatctcatt aaaggttata gcaatattca aggtaactta gtaacagcca ttcctaactt
    38221 cctggagaaa gttaataatg attttgctat aagtgctaga aatagagact tatatagtca
    38281 ctaccttcct tatcagggta ttgatgattg ggagaattct aattttaact ttcgaacact
    38341 aaatggagtt tattatgatg ataatctaac ttcaacagaa ctcctgcgaa aatttatgat
    38401 tcaaagagat tataacactc tttatgctct aagttctgct cgcacaggaa atgctgatag
    38461 tctggctaaa aattaccatc ttgtagttgc gaaaatgtcc tatgaaaacc tgaaaaaaga
    38521 attatccaag aaacccgact ctacatttaa taaactgaaa aagctgtatg aaaccaatgg
    38581 tgaattctta aatgaatata ttttagacaa tttccgagga aatccacttg aattattaac
    38641 agtacctgcg cttaaagaaa gtttagaaaa ccaagagtca ttccctagca ggctgtatca
    38701 gaaatttaat attttc
//
LOCUS       DANLUQ010000005        35507 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.005, whole genome shotgun sequence.
ACCESSION   DANLUQ010000005 DANLUQ010000000
VERSION     DANLUQ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 35507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35507
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.005"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(35..2977)
                     /gene="rne"
                     /locus_tag="PXB27_000210"
     CDS             complement(35..2977)
                     /gene="rne"
                     /locus_tag="PXB27_000210"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HDL5345045.1"
                     /translation="MKRMLINATQKEELRVALVDGQRLFDLDIESPGHEQKKSNIYKG
                     KITRVEPSLEAAFVDYGAERHGFLPLKEISREYFPSDYVFNGRPNIKDIIQEGQEVIV
                     QVSKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDERLELKEALDCLDV
                     PEDVGLIVRTAGVGKSPEELQWDLKVLLHHWEAIKKAAESRPAPFLIHQESDVIVRAI
                     RDYLRRDIGEILIDNKKVFEKAKNHIRLVRPDFINRVKLYEGEVPLFSHYQIESQIES
                     AFQREVRLPSGGSIVIDVTEALTAIDINSSRSTRGGDIEETALNTNLEAADEIARQLR
                     LRDLGGLIVIDFIDMTPVRHQREVENRIREATRQDRARIQFARISRFGLLEMSRQRLS
                     PSLSEASSHICPRCQGTGKVRDNESIALSILRLLEEEAIKENTAQVHTIVPVQIASYL
                     LNEKRKAISSIEKRHDVQIVVVPSESMETPHFSVYRLRENEITPTLSYDLTKHYEARH
                     EEEHPHHHHQEDVLVTRNEPVITVESVLQSSELNLQPSPTPTEPPKPSLLSRLIATIK
                     GWFSEEKVEEKPKPKARNQRERRQRRERPNRNAKAENQERKTPQAQTTPAEEKPMRSE
                     KKARRAVVEEVSGVTEQESRSNSREVQTERRQRRDLRKKVRIETQEVEVENLTPAVEE
                     TVAKQVENVQKNQQIQPLATQEVPVETAPEVETTNEGRENNRQRRLPRHLRVGNQRRR
                     ENQRNQVISPMPLTSAVASPELASGKVWVQVTSTVKVEEKASFLSVDQMLEQQENQLV
                     ETEKKSEEKKLHTVSPLGGFVAQATNRDLVKEKQEQLSKPKEEKVASEDVKPVEVKEH
                     IAPFGSVVAQHSERDLVKEKNERVSKFQSQATVAPAIVEEVTNPIEVPKSTYESDYRF
                     NGRLGTFSRVEHTQAAMTIASAPSDKLEVVEISQWQNSKYYFYGKGFGGYTSAVSQVQ
                     SAPRSTNG"
     gene            complement(3434..4279)
                     /locus_tag="PXB27_000211"
     CDS             complement(3434..4279)
                     /locus_tag="PXB27_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL5345046.1"
                     /translation="MMSQEQFEEINQERRQALNHGSRIAMAASVLGALGIASTANAKQ
                     ASAEKSPYAEPEQYGLKRHGMTIDPKRVALVVVDPQIDFLGPKGVMWGVLGDSIKENN
                     TVANIESLFKAAKAVDMPTFVSPHYYYPTDHKWEFGSPGEHFMHDSGMFARKGQYTME
                     GFENSGADFMPEYKPYIFDGKTVITSPHKIFGPETNDLVLQLRKRKIDQVILAGMAAN
                     LCIDSHLRELIEQGFEVTVVKDATAGPRIPEGDGYLAALTNYRIIANDLWTTEEAVKK
                     MTAKA"
     gene            4406..5230
                     /locus_tag="PXB27_000212"
     CDS             4406..5230
                     /locus_tag="PXB27_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL5345047.1"
                     /translation="MDSLTHLFTQFQFRTDLFHLGQLCQIGSFNEENKGYLHFVRQGR
                     FILNQAAERPITIERPAIIFSPTNILHSLHPLDDSGLDIFCINFDFGKGIRNPLTHTL
                     HNIVILELAQYPELGVLADQIFSEVNQKGCGYQAMIHHLCAYFTLKVARQCLEKGYIQ
                     TGLLKGLADKQLGKVLLAIHNAPERDWNVEELANVALMSRSRFAAYFKQTMGVSPMDY
                     LTNWRLAVAQHLLQKGIPVALVAEQVGYSHNAALSRVFMRELGLSPTEWLAQNKES"
     gene            complement(5315..6778)
                     /gene="ubiD"
                     /locus_tag="PXB27_000213"
     CDS             complement(5315..6778)
                     /gene="ubiD"
                     /locus_tag="PXB27_000213"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HDL5345048.1"
                     /translation="MKYKNLREFLDFLESQGELVRIQQEIDPYLEMTEIADRTLRKGG
                     PALLFENPKGYQMSVLCNLFGTPKRVALGMGQEDTKALRELGKLLAFLKEPEPPKGFK
                     DLIGQLPQWKQILNMPPKVLSKADCQQVVISGDEVDLYKLPIMHCWPGDVAPLVTWGL
                     TITQGPYKKRQNLGIYRQQLIGKNKLIMRWLSHRGGALDFQEWKAENPGKPFLVSVAI
                     GADPATILAAVTPVPDTLSEYAFAGLLRGHKTEVVKSISNELEVPASSEIVLEGYIDL
                     EETALEGPYGDHTGYYNEQEYFPVFTVTHITMRKDAIYHSTYTGRPPDEPAVLGEALN
                     EVFIPILQKQFPEIVDFYLPPEGCSYRLAVVTMKKQYAGHAKRVMMGVWSFLRQFMYT
                     KFVIVCDDDVNARDWKDVIWAITTRCDPARDTTLIEHTPIDYLDFASPIAGLGSKMGI
                     DATNKWAGETTREWGTPIEKDDNVVKRIDEIWDSLGI"
     gene            6915..7889
                     /gene="znuA"
                     /locus_tag="PXB27_000214"
     CDS             6915..7889
                     /gene="znuA"
                     /locus_tag="PXB27_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HDL5345049.1"
                     /translation="MFKRTLIGLAVLGASVAQADVLTSIKPLGFIASAITEGVTETKV
                     LLPVTASPHDYSLKPSDVTQLQSAQLVVWVGDGLEGFLEKSIEKLPREKVLTLEDVDA
                     IDDIVDKASKKEDKDDDHDHKHEHKHEHKHEHKHEGHNHEGHNHDHHGHSHDEDWHIW
                     LSSEASELAAEQIAARLSEQMPEHKAKIAENLAKFKANLTAKKAEIAKQLEPVKAKGY
                     YTFHDAYGYFEREYGLNSLGSFTINPTVAPGAKTLTAIKKNIAKNKAQCLFAEPQFTP
                     KVIETLAKETKVNVGQLDPLGAKVQLSATAYPEFLQVLADEFSQCLTK"
     gene            complement(7941..8261)
                     /locus_tag="PXB27_000215"
     CDS             complement(7941..8261)
                     /locus_tag="PXB27_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005621693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnA domain-containing protein"
                     /protein_id="HDL5345050.1"
                     /translation="MIYPTCPECKSENTYHDSIQFVCPDCAYEWTGEETDQDDDVLIV
                     KDSNGNLLSDGDDVLLIKDLKLKGSSEVLKKGTKFKGIRLAQGDHNVDCGKIMLKSEF
                     LKKA"
     gene            8402..10765
                     /gene="rnr"
                     /locus_tag="PXB27_000216"
     CDS             8402..10765
                     /gene="rnr"
                     /locus_tag="PXB27_000216"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDL5345051.1"
                     /translation="MIVDPNYQQELEKYENPVPSREFILNTIREHNAPMSRDELLSAF
                     HIDDEERMEGIRRRLRAMENDGELVFTKGKRYALPEKMDLIKGTVIGHRDGYGFLQVE
                     GHEKGATKSEDWFIPNAQMVKVMHGDFVLAQPNGTDRRGRKEVRIVRVLEARKKQIVG
                     RFFLESGIGFVVPDDSRVNQDILIPDEHRMGARMGQVVVVELQERKASFSRPVGIITE
                     ILGDNLAPGMEIEIALRNHDIPHIWPEGVEKQIRQFSSEEVPEEAKQGRVDLRDLPLI
                     TIDGEDARDFDDAVFAKKEGNGWRLWVAIADVSYYVRPKTALDLEALNRGNSVYFPNR
                     VVPMLPEVLSNGLCSLNPQVDRLCLVAEMSVSSKGELQDYQFYEAVMNSHARLTYTKV
                     WKMLEGDEALRERYAPLVPHIEELYAMFQTLMKARHKRGAIEFETIENQFIFNPQGRI
                     ERIEPLIRNDAHKLIEECMILANIAAARFVEKANEPALYRIHDKPSEEKLLSFKSFVR
                     ECGLTWDVGLDPTPKDYGVLLEQIAERADKELIQTMLLRSLKQAVYAADNIGHFGLAL
                     TEYAHFTSPIRRYPDLLLHRAIKYLIEKGKGNTRYYTDGGGYHYKLDDMDEFGDKCSA
                     TERRADEATREVADWLKCEFMQDHLGEEFDGVISSVTGFGLFVKLNELLIDGLVHIST
                     LDNDYYHYDADRQRLIGGSGIIYRLGDAVRVKVINVNLDEKKIDFELLTGNKKTGKTA
                     KKKAKATEVPVFKEPKTVKKAKKVAEKKPAKSAKKRTKTTASKSRKK"
     gene            10769..11035
                     /locus_tag="PXB27_000217"
     CDS             10769..11035
                     /locus_tag="PXB27_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345052.1"
                     /translation="MDYKKQILFSLLFALVIAIISYFLFTYIPFDCSDLSKLNDSQRL
                     ECRTAEFLGVYYWLPHTTTLFFGSVLLYWAVYGMISLVQAVLKR"
     gene            11258..11716
                     /gene="mraZ"
                     /locus_tag="PXB27_000218"
     CDS             11258..11716
                     /gene="mraZ"
                     /locus_tag="PXB27_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDL5345053.1"
                     /translation="MFRGASSISIDSKGRIAIPTRYRAELLEKHHGILVCTVDIRQPC
                     LLLYPLHEWEMVEQKLLALSNFDPVQRRIQRVMQGFATECEMDSAGRILLSPTLRQHA
                     QLEQQIMLVGQLNKFEIWQDKQWQAQIAEDLAFGSSAEILDCEALKNLSL"
     gene            11732..12670
                     /gene="rsmH"
                     /locus_tag="PXB27_000219"
     CDS             11732..12670
                     /gene="rsmH"
                     /locus_tag="PXB27_000219"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDL5345054.1"
                     /translation="MSENPKHITVLLHEAVDGLAIKPNGIYIDGTFGRGGHSRLILSK
                     LGENGRLIATDRDPRAIAEAQTISDPRFQIEHTAFSAIPELCERMGLVGKIDGILLDL
                     GVSSPQLDEAERGFSFMRDGPLDMRMDTTKGLSAAEWLAQVSAEDLAWVLKEFGEERF
                     AKRIAQAVVSYNKSANEKISRTLQLAQIIADAVPFKDKHKHPATRSFQAIRIFINGEL
                     DELEKALNSALTVLAPEGRLSIISFHSLEDRMVKQFVRKHSKGMDVPRGLPILEAELT
                     KNIPLKTIGKAIMPSEAEIEANARSRSAVLRIAEKR"
     gene            12689..13000
                     /gene="ftsL"
                     /locus_tag="PXB27_000220"
     CDS             12689..13000
                     /gene="ftsL"
                     /locus_tag="PXB27_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDL5345055.1"
                     /translation="MSNERYPLHQIIIDDIFSHNKVALLLLIGVVISAVATIWITHQT
                     RLSVSEQGQLIQANQKLESQYTNLQLEENSRSSRARVDAAAKSFGLQPIKKEQEIILV
                     E"
     gene            13004..15064
                     /locus_tag="PXB27_000221"
     CDS             13004..15064
                     /locus_tag="PXB27_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsI"
                     /protein_id="HDL5345056.1"
                     /translation="MVKSVKLKRKQEAPVAKAEPQQAEGKKTPSFLPTRFRVVVFFML
                     LMVGGLLAQTAYIQLFDSERLIKEANNRSLRTKELQFTRGRILDRNGRFLSISVPMYS
                     LTLDPREYFDTKLRRSNESWRALAIEMETSKGKIEANVNKFIEKKNFSKEKVDFDPRS
                     ILNTKSEGYWTQLAQVTGLDYNSLIEKVRNNPSSVFLRLDNENLVRERQKFQALSKEI
                     GRSYNDIMDELYSKNRQRFLYLSRHQSEAISEYAKELDIDALVIKVESRRFYPLAEES
                     SQLIGFTDKDDMHGSEGLERSFDSLLIGKNGKQIIRKDAKGNIVENIRSEKQYDPQDV
                     MLSIDEELQSMVYGEIKKAVQENNAESGTAVLVDVQTGEILAMANAPSFNPNKRDSFK
                     PELMRNRAITDTFEPGSTVKPFTVLTALQNGVTYRDEVIGTRPFVVNGHTIKDVAPRD
                     SLTLTGILQKSSNIGVSKLALRMPANALVDTYTKVGFGKDTGLGLGEQRGTNGDRKRW
                     ADIERATLSYGYGLNVTPLQLARAYATLGSFGLYRPLSITKVDPPVIGERVLPEKTTR
                     DVVHMMESVAAKGEGGHRAAVDGYRVAIKTGTARKLEKGKYVEKYIAYTAGLAPASDP
                     RFALVVLINEPKAGNYYGGFVSAPLFSRIMGYTLKQRNIKPDNLTENTQSAVREIKLE
                     RPIN"
     gene            15073..16563
                     /gene="murE"
                     /locus_tag="PXB27_000222"
     CDS             15073..16563
                     /gene="murE"
                     /locus_tag="PXB27_000222"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HDL5345057.1"
                     /translation="MKRLLPFLTELDAWVEELTELKQMTLDSRQVQAGDLFVALKGHQ
                     VDGRKFIPNAIEQGAAIILAEADEDQPEIELDPKFAKFNLDRTACCKVVSVPRLPKLL
                     SEIAGALYHNPSHKLTLAGITGTNGKTTTAQLLAQWHNLLGGKSAVMGTIGNGLYGQV
                     QEAVNTTGSAIEIQHNLANFVELGADFCAMEVSSHGLAQFRAEALDFDLAIFTNLSRD
                     HLDYHNTMEEYAQAKFRLFNELSTKAQVINADDEIGREWLTQLPNAVAVSTDPKFAGN
                     HQFVKATAVKFTLQGASIAFESSWGNGELHSRLIGAFNVNNLLTAFAGLLALGFDIQE
                     LVKTAPNLVGVAGRMECITAPNKPMVIVDYAHTPDALEKALQAARLHCEGELWCIFGC
                     GGDRDAGKRPLMATIAEKLTDKVIATDDNPRTEDNQKIMADIVKGFSKPQQIIHNREE
                     AIKTAIEQAKATDVILIAGKGHEDYQIIGTEKLHFSDQETARKYLF"
     gene            16651..18045
                     /gene="murF"
                     /locus_tag="PXB27_000223"
     CDS             16651..18045
                     /gene="murF"
                     /locus_tag="PXB27_000223"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDL5345058.1"
                     /translation="MIKLTTQQIAEILNAKLIGNGETVVETTSTDTRQAVENGLFFAL
                     KGENFDAHHYLQNAVAQGCVAVVVERECEIDVPQLVVADTRLALGELAKWLKAKLNPK
                     TVAMTGSSGKTTVKEMTAKILQKMTACEQEVLYTFGNLNNDLGVPMTLLRLTEQHKYA
                     VIELGANHIGEIAYTTAIAQPDACLVNNVAAAHLEGFGSLAGVAQAKGEIYRGLKENG
                     KAIVNLAFYYPQWQKEIGSREMQSFSYIGSDSDSYADYWAEDVELHLSGSRFTLHTPQ
                     GEIAIDLPYLGKHNVSNALAATSLAMSVGATLEAVKAGLEQRSMVKGRLYPVQINDHC
                     LFIDDTYNANVDSMKSAISVLENYPAYRIFAVGDMAELGEESSKCHQEVADFAKNAAL
                     DLIVSFGKESAVISQQAEHHFTDKQQMVDFLLPIILQKIEQKQPLVLLAKGSRSQKME
                     TVISELCKPFNLNF"
     gene            18066..19148
                     /gene="mraY"
                     /locus_tag="PXB27_000224"
     CDS             18066..19148
                     /gene="mraY"
                     /locus_tag="PXB27_000224"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDL5345059.1"
                     /translation="MLVWLAELLVQYNTAFNVVSYITFRAIMALLTAMGIGLWIGPKV
                     IRRLQILKFGQEVRNDGPESHFKKRGTPTMGGVMILAAIGVSTLLWADLRNSYVWFTL
                     FVLFGYGAVGFVDDYRKISRKNTDGLIARWKYFWLSAIALVAVFGMYAVGKDTAATQL
                     VVPFFKDVMPQLGLFYIVLSYFVIVGTSNAVNLTDGLDGLAIVPTIMVASAFALIAWA
                     TGNFNFAQYLHIPFIPNAGELVILCTAIVGAGLGFLWYNTYPAQVFMGDVGSLSLGGA
                     LGVIAVLVRQELLLVVMGGVFVVEALSVILQVGSYKLRQKRIFRMAPIHHHFELKGWS
                     EPRVIVRFWIITLMLVLVGLVTLKLR"
     gene            19212..20513
                     /gene="murD"
                     /locus_tag="PXB27_000225"
     CDS             19212..20513
                     /gene="murD"
                     /locus_tag="PXB27_000225"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDL5345060.1"
                     /translation="MQKQYENKTVTIIGLGTTGLSCVDFFKKKNANIQVIDTRANPAG
                     ADKLDVTIPLHTGGLNSEWLLKSDLIVISPGLAVATPEIQQAIQAGVEIVGDVELFCR
                     EAKAPIIAITGSNGKSTVTTLTTEMAKQAGIKVGMGGNIGVPVLSLLEGDYDLFVLEL
                     SSFQLETTYSLKAKAATVLNISEDHMDRYDSIADYRQAKLRIYDNAENIIVNGEDAQT
                     YPIQAVKKVIRFAEQNAEYSLRNQMLYSGDDAIIQTKQMLISGRHNEMNALAAMALAE
                     AANVPREGIVKALQIYGGLPHRFQAVATNDGVRWVNDSKATNVGSTVAALNGLNIAGT
                     LYLLLGGDGKAADFSELKPLINKPNIVCYCFGQDGAALAELSEQSVLVETMQQAIEAI
                     RPQLKKGDMVLLSPACASLDQFKNFEQRGEVFMALAQGQHA"
     gene            20513..21691
                     /gene="ftsW"
                     /locus_tag="PXB27_000226"
     CDS             20513..21691
                     /gene="ftsW"
                     /locus_tag="PXB27_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HDL5345061.1"
                     /translation="MLERIKSEWDKWVRLTPSNSLYDRTLIWLFLGLLIIGFVMVTSA
                     SLPVSTRLNNDPFYFAIRDGLYIIASIIFCYVFVQIPIEKWEKHNLALFFISIGFLIA
                     VLIFGRSINGAVRWIPLGILNFQPAELAKLAVICYFASFYVRKYDEIRKEKASFWRPA
                     VILFLFGFLLILQPDLGSTFVLFVLTFSMLFIVGAKIMQFMFLGVVGTVLFAVLILTS
                     EYRLKRVTSFMDPFADAYGDGFQLSNSQMAFGQGEFWGQGLGNSVQKLEYLPEAHTDF
                     VMAVIGEEFGFFGIACIVLLLILLTVRALKISKESLVLEERFKGYMAFGIAIWVFLQG
                     FVNLGVASGLLPTKGLTFPLVSYGGSSLVIMSIAIAVLLRIDHENRAERIGHAKIKTI
                     "
     gene            21810..22865
                     /gene="murG"
                     /locus_tag="PXB27_000227"
     CDS             21810..22865
                     /gene="murG"
                     /locus_tag="PXB27_000227"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDL5345062.1"
                     /translation="MAKKLLVMAGGTGGHVFPAIAVARELQKQGWQIRWLGTKDRMEA
                     TLVPKHGIEIDFIEISGLRGKGVAALLKAPFAIFKAVMQARKIIKNYQPDAVLGMGGY
                     VSGPGGIAAKLCGVPVILHEQNAVAGLTNVWLSKIARKVLQAFPTAFKEAEVVGNPVR
                     EDLSALPLPNERFAKRGYPINILVMGGSQGARVINQTVPEVAKVLGNNVFISHQVGKG
                     NLAGIEEVYQQTGNGIASEFIDDMKAAYEWADLVICRSGALTVCEIAAVGLPAVFVPF
                     QHKDRQQFLNANYLAADGAAVIIEQSDFTPKALQQALEPLIANRQLLLDMSMRARAKS
                     TPMAAKRVAEVIIQEAN"
     gene            22941..24368
                     /gene="murC"
                     /locus_tag="PXB27_000228"
     CDS             22941..24368
                     /gene="murC"
                     /locus_tag="PXB27_000228"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013527829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDL5345063.1"
                     /translation="MKNFQDKVKKLVPEMRRVSQIHFIGIGGAGMSGIAEVLLNEGYQ
                     ISGSDIADRAVTQRLSAAGAKVFIGHQAENIAGASVVVVSTAIDETNPEIIAAREARI
                     PVIRRAEMLAEIMRFRHGIAIAGTHGKTTTTAMISMIYTEAKLDPTFVNGGLVKSTGK
                     NAHLGASRYLIAEADESDASFLHLQPMVSVVTNIEPDHMDTYGGDFEKMKETYVRFLR
                     NLPFYGLAVMCADDETVMEIAPRVGRQVITYGFSENADYRIEDYQQTGFQEHYTVVCP
                     SGERIDVLLNVPGMHNALNATAALAVAKEEGIGNEAILAALADFQGAGRRFDQLGSFI
                     RPNGKIMLVDDYGHHPTEVDVTIQAARSGWEGKRVVMIFQPHRYSRTRDLFDEFVQVL
                     SKVDALILLDVYAAGEAPIVGADSKALARSIRNLGKVDPILVSDTDQLGEVLDQIIQD
                     GDLILAQGAGSVSRISRGLAESWKN"
     gene            24458..25369
                     /locus_tag="PXB27_000229"
     CDS             24458..25369
                     /locus_tag="PXB27_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDL5345064.1"
                     /translation="MSIKNEKIAVLFGGVSQEREVSLNSGAAVTAALKSLGYNVEGID
                     TKEFPIEKLKEKGIQRVFNILHGGIGENGVLQGALEQMGIPYTGCGVMASAVTLDKFR
                     TKLLWNAVGLPTADMVVVQRGQAVDFDQIIAKLGLPVFVKSSCEGSSVGVFKVKTKEE
                     LAPAIHEALKFDSIVLVEEFLAGNEYSVPVLDGQVLPAVQVIPDGEFYDYHAKYVSDN
                     TQYVVPALNDERQAEVAKLVKAAYDVVGCRGWSRIDVMEDGEGKFRLVEVNTNPGMTS
                     HSIFPKSAATVGISFEKLVERVLELSA"
     gene            25369..26160
                     /locus_tag="PXB27_000230"
     CDS             25369..26160
                     /locus_tag="PXB27_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HDL5345065.1"
                     /translation="MGIFRKKNSTNVRLNVLNPKTPVSPRKWLVFIKPLIVLLCTLFV
                     FGVYSNWQNILESWDKTPIRAYALTHKTQFTTNADIRETLSKEPALKGYFGQNIQEVK
                     DKLLSIPWVKDVAVRKLYPDRLSITLLEHKPVAIWNDTNFVSEQGTVFSLPKDRIDKN
                     GLPLLYGPDTEGKSVLEAWNKIRADLNARNLDLTSVSVDNRGSWTIRLSNQVELRLGR
                     GEWTSKIDRFVTIFPEIHIPEGNRLAYVDLRYPHGAAVGFSPIKN"
     gene            26183..27454
                     /gene="ftsA"
                     /locus_tag="PXB27_000231"
     CDS             26183..27454
                     /gene="ftsA"
                     /locus_tag="PXB27_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDL5345066.1"
                     /translation="MTKAVESKIIVGLDIGTSKVVAVVGEVLPDGVINVMGAGVCPSK
                     GVDRGGVIDLDAAVNSIQRAIEQAESIADCQIMGVTLAISGQHIVGLNEAGSSTLSGG
                     IVTQDNIESAVDMAKAIKLQDGLETLHMIPQEYHVDRLPATKNPIGLSGMRLQVQAHL
                     IACHQAWLKNLKNAVEHAKLKVDQVVFSGLASSYSVLTEDEKELGVCLIDIGGGTMDV
                     LVYTDGALRYSKVIPFAGNNITDYLARVFTTSRPEAESLKVGYGSAISPPTHNSDKKI
                     EVAGLGGRMARTFTRAQVATVTSQCYNDLLKVVEEELTQLRHELFQKGIKQELIAGVV
                     ITGGGSQIEDIVECAKSIFGSQVRVGYPLNITGLTDYVNKPQYATALGLLQYSHYNYT
                     EQGTSTDSNEDIFAPIGRGTKKIIDFFKSKF"
     gene            27487..28734
                     /gene="ftsZ"
                     /locus_tag="PXB27_000232"
     CDS             27487..28734
                     /gene="ftsZ"
                     /locus_tag="PXB27_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDL5345067.1"
                     /translation="MFEPIIEQTQDAIIKVVGVGGGGGNAVDRMSRSADDIKGVEFFD
                     VNTDAQVLRKRTTRQTIQIGASTTKGLGAGADPMVGKQAAEEDREAIANALKGANMTF
                     IAVGMGGGTGTGAAPVVAQIAKEQGSLTVGVVTKPFRFEGPRRMRFADQGIKELSQYV
                     DSLIIIPNDKLRGLGKQTTAVDAFAAANDVLSNCVLGITNMITSSGGSTGADINVDFA
                     DVRTVMSGKGHAMIGTGFAEGEVGEGRAEKAMNDAISSPLLENVDISGASGMLINISA
                     GTDFLLEEVYAMMDLIYGFATEDAAIVFGCNYYPEMDGKVSVTLVATGIGQPEEALHM
                     PHKAQPVYAQQGNHVQATQPTQPNHAVQQPNFTQPSSYGQPNQFSGQPQPLQQPVAPK
                     PQSVDTAQIFNPNSIPGFMRNSQ"
     gene            28766..29686
                     /gene="lpxC"
                     /locus_tag="PXB27_000233"
     CDS             28766..29686
                     /gene="lpxC"
                     /locus_tag="PXB27_000233"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012073651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HDL5345068.1"
                     /translation="MTKQRTLKQAAKVTGIGLHSGKKVTLTLRPAPANTGVIYARTDL
                     EPVVYFPASADSIRDTQLCTCMVNDDGVRISTVEHLNAAMSALGLDNVIVEVDAPEIP
                     IMDGSSSPFIYLLLDAGIEEQDAPKKFIRIKETVRVEEGDKWAELKPYNKGFKLNFTI
                     DFEHPLIPKNVGSYSIDFSAENFIQQLSRARTFTFMKDVEYLQSIGLALGGSLDNAIV
                     LDDYRILNEDGLRFKDELVRHKMLDSIGDLYMCGYNILGEFSAYKSGHGVNNKLLRAV
                     LENKNAWEFVTFEDKAEVPQGYQVQQEIFI"
     gene            29859..31541
                     /gene="recN"
                     /locus_tag="PXB27_000234"
     CDS             29859..31541
                     /gene="recN"
                     /locus_tag="PXB27_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDL5345069.1"
                     /translation="MLTQLTINNFAIVRHLILELNEGMSVITGETGAGKSIAIDALGL
                     CLGYRSESGMIRNGSDKADISATFTMQPHSPAYLWLQEHELLDEDNPQECILRRMINI
                     DGRSKAFVNNRSLPVSQLRELGQYLIHLNGQHAPQLLLKNEYQLEIVDHYATLQPLLM
                     KMAKQYGNWKELNKQVKNFQKQCQENEARKQLLEYQVEELDEFAIKANEFEEMEEKYN
                     ILSSSEELTELSDEITRLLSDDEINANSLIYKALRNAENLAEVDSQYQGILDMLNEAL
                     IQIQEASSEVAQLASKIEQDPELLQELDNRIAQTISLARKHHIQPNELWQYHATLKAE
                     LQELLDFEDNEEQLIQNEKQAYQECITLAEEIYQKRLDATHKLSEQVTKQIKHLAMEN
                     GEFFIHIEHNLEKMSANGADYVEFNLRSNLGQQPQPLAKIASGGELSRISLAVQVLTA
                     NKLSTPTIIFDEVDVGISGSAATTVGKLLRKLGEKCQVLCVTHLPQVASYGNQQFNVQ
                     KYVENNQTETQMTLLTEAERIHALARLLGGSEITETVLANAKEMLNLAKNNE"
     gene            complement(31588..31663)
                     /locus_tag="PXB27_000235"
     tRNA            complement(31588..31663)
                     /locus_tag="PXB27_000235"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31628..31630),aa:Ala,seq:ggc)
     gene            complement(31743..33182)
                     /gene="gltX"
                     /locus_tag="PXB27_000236"
     CDS             complement(31743..33182)
                     /gene="gltX"
                     /locus_tag="PXB27_000236"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007526628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDL5345070.1"
                     /translation="MKIETLFPLDPNVKVRTRFAPSPTGYLHVGGARTALYSWLYAKH
                     NDGEFVLRIEDTDLERSTPEATAAILEGMEWLDLAWEHGPYFQTKRFDRYNQVIDQML
                     EAGTAYRCYCSKERLDEVRAEQEANKEKAKYDQHCLHNPQNDPSQPHVVRFKNPTEGS
                     VVFDDAVRGRIEIANSELDDLIIRRTDGSPTYNFCVVVDDWDMGITHVVRGEDHINNT
                     PRQINILKALGAPIPTYAHVSMINGDDGQKLSKRHGAVSVMQYRDDGYLPEALINYLV
                     RLGWGHGDQEIFSRDEMIELFDIHSVSKSASAFNTEKLQWLNQHYIRSLAPSYVAKHL
                     EWHMQTQGIDFTSGPKLEEIIPVLGERCKTLKELAESSRYFYQEFKAYDEKAAAKNFK
                     AEAVAPLAKLLEKLTACNDWTVENIHEAMNQTAAELEIGMGKVGMPFRLAVTGSGQSP
                     SMDITAKLVGKERTLARLQKAIEFIQANS"
     gene            33357..33432
                     /locus_tag="PXB27_000237"
     tRNA            33357..33432
                     /locus_tag="PXB27_000237"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33390..33392,aa:Val,seq:tac)
     gene            33481..33556
                     /locus_tag="PXB27_000238"
     tRNA            33481..33556
                     /locus_tag="PXB27_000238"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33514..33516,aa:Val,seq:tac)
     gene            33609..33684
                     /locus_tag="PXB27_000239"
     tRNA            33609..33684
                     /locus_tag="PXB27_000239"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33642..33644,aa:Val,seq:tac)
     gene            33733..33808
                     /locus_tag="PXB27_000240"
     tRNA            33733..33808
                     /locus_tag="PXB27_000240"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33766..33768,aa:Val,seq:tac)
     gene            complement(33962..35455)
                     /gene="mltF"
                     /locus_tag="PXB27_000241"
     CDS             complement(33962..35455)
                     /gene="mltF"
                     /locus_tag="PXB27_000241"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HDL5345071.1"
                     /translation="MKGLIARLFIAVALLLWAWDMIFPWQKIIHSEANHYTQIQQSKT
                     LKVGMINHPLSYFVGPEGKSGIEYDLATAFAQYLKVNLDIKTYDSSEKLFEALRNNDV
                     DIAAAGLLYHSELNDEFQIGPSYYSASWQLVYKKGSARPYRLSDLRENVIIPAGSAVI
                     PILNQLKVDNPELKWQISDKFTQEELLLKVAQGEISHTIAMSVDISAAQHINPNLAVG
                     FDLTDENPVLWYLANSSYSELQSVLLDFMSYSMETGLISRIEEKYFNHLAKFDYVDIQ
                     SYLNAIKTILPKYEPLFRAHKGELEWQMLAAIAYQESHWDPNATSPTGVRGIMMLTKD
                     TADRMKISDRTNPAQSIKAGSEYLHMLMRQMPETIPQADRIWYSLAAYNMGLGHLLDL
                     RRLTKQLGGDPDNWLDVKKNLPLLAEKRYYANLKYGYARGYEALQYVENIRRYYGSII
                     NYQRVEEQKMQEQENLPDGQIKQENSSYEKIEEKEIPTDKEITPASN"
BASE COUNT        10414 a         7004 c         7875 g        10214 t
ORIGIN      
        1 ataactaata ggcatttaaa ccaattaatt gtacttaacc attggttgaa cgaggcgctg
       61 actgcacttg gctcactgcg ctggtatatc cgccaaaccc tttgccatag aaatagtatt
      121 tagagttttg ccattggcta atttcgacta cttcaagctt gtcacttggt gcagaagcaa
      181 tggtcatagc ggcttgggta tgctcaacac gagagaatgt acctaatctg ccgttaaatc
      241 gataatcgct ttcgtaagtt gattttggca cttcaatcgg gttagtaacc tcttccacaa
      301 tcgctggtgc aaccgttgct tgcgattgga atttactgac tcgctcattt ttctctttaa
      361 ctaaatcacg ttcagaatgc tgagcaacaa cactaccaaa tggagcaata tgctctttta
      421 cctctactgg ttttacgtct tcagaagcaa ctttttcttc tttcggtttg gaaagctgtt
      481 cttgcttctc cttaaccaaa tcacggttag ttgcttgtgc tacaaagcca cctagcggac
      541 taactgtatg aagcttcttc tcttctgatt ttttctctgt ctcaacaagc tgattttctt
      601 gctgttcaag catttgatca acagataaga atgatgcttt ttcctctact ttgacagtag
      661 aggtaacctg aacccaaact ttaccgctcg caagctcagg cgaagcaaca gctgaagtta
      721 gaggcatagg agaaataacc tgattgcgtt gattttcacg acgacgctga ttgcccacac
      781 gcaaatggcg aggtaaacgg cgttggcggt tattttcacg tccttcgttc gttgtttcta
      841 cctcaggagc tgtttcaaca ggtacttctt gggttgcaag cggttgaatt tgttgatttt
      901 tttgcacatt ttcaacttgt tttgccaccg tttcctcaac agcaggtgtt agattttcca
      961 cttctacttc ttgtgtttcg atacgcactt ttttacgtag atcacggcgt tggcgacgtt
     1021 cagtttgaac ctcacgggag ttgctacgac tctcttgctc ggttacaccg gaaacttctt
     1081 ccactaccgc tcggcgtgct tttttctcgc tacgcatcgg tttttcttcc gctggagtag
     1141 tttgagcttg cggagtttta cgctcttgat tttccgcttt agcattacgg ttcgggcgtt
     1201 cacggcgttg gcgacgctca cgctgattgc gtgctttcgg cttcggtttt tcttccactt
     1261 tttcttcact aaaccagcct ttaatggtcg caattaagcg gcttaacagc gatggttttg
     1321 gtggttcggt tggtgttggc gaaggttgga gattcagctc cgagctttgt aacactgact
     1381 ctaccgtaat caccggctcg ttacgggtaa ccagcacatc ttcttgatgg tgatgatgtg
     1441 ggtgttcttc ttcatgacga gcttcgtaat gcttggttaa atcgtagctt aacgttggag
     1501 taatttcatt ttcacgcagg cgatatacgc tgaaatgcgg cgtttccata ctttcagacg
     1561 gtactacaac aatttgtaca tcgtggcgtt tttcaatgct ggaaatcgct ttacgttttt
     1621 cgttgagtaa gtagctggcg atttgcaccg gcacaatggt gtgaacctgt gcagtgtttt
     1681 ctttgattgc ctcttcttct aataaacgta agattgacag tgcgattgat tcattatcac
     1741 gtactttacc agttccctga cagcgagggc agatatgcga tgaagcctca cttaatgatg
     1801 ggcttaaacg ctgacggctc atttctaaca aaccgaaacg ggaaatacgg gcaaattgga
     1861 ttctcgcacg gtcttggcgt gtcgcctcac gaatacggtt ttctacttca cgttggtggc
     1921 gaaccggtgt catatcgata aagtcgatca caatcaaacc acctaagtca cgcaaacgta
     1981 actgacgggc aatttcatct gccgcttcta agttggtgtt aagagcggtt tcttcaatat
     2041 cgccgccacg ggttgaacgt gaggagttaa tatcaattgc tgttaaggct tctgttacat
     2101 caataacgat agagccaccg gaaggtaaac gcacttcacg ttggaatgcc gattcgattt
     2161 gcgattcaat ttgatagtgg ctgaatagtg gtacttcgcc ttcgtataat ttcacacggt
     2221 tgatgaaatc cgggcgaaca aggcgaatgt ggtttttggc tttctcgaac acttttttgt
     2281 tgtcgattaa gatttcgcca atatcacggc gtagatagtc acgaattgca cgcacaatca
     2341 cgtcactttc ttggtgaatt aagaacggag ctgggcgaga ttccgccgct tttttaatcg
     2401 cttcccagtg gtgtaacaag acttttaagt cccattgtaa ctcttccggc gatttgccga
     2461 caccggcggt acgaacgatt aagcccacgt cttccggtac atctaagcag tctaaagcct
     2521 ctttgagttc taaacgctca tcaccttcaa tacggcggga aataccaccg gcacgagggt
     2581 tgttcggcat taagactaag taactgcccg caagcgaaat aaaggtggtt aaggctgctc
     2641 ctttgttacc acgctcttct ttgctgactt gaacaatcac ttcttgccct tcttgaataa
     2701 tatctttgat attcgggcga ccgttaaaca catagtctga cgggaagtat tctcgggaaa
     2761 tttcttttaa aggtaggaag ccgtggcgtt ctgcaccgta atctacgaat gcagcttcaa
     2821 ggcttggttc gacacgggta attttaccct tgtagatatt ggattttttc tgttcgtgac
     2881 ccgggctttc aatgtccaag tcaaataagc gttgcccatc aacgagggca acacgcaact
     2941 cttctttttg agttgcattg attaacattc ttttcattgt gttttctcat tattttgttt
     3001 ttgtcttaca cggtcatttt gacccattaa taagggaatg gatttaatgt ctatctcacg
     3061 actgtcttca ttcccgatag tgcattgatg gttgtcttac gtttactttg tcttgtctta
     3121 aagaaactgc aaaatcaatc acttaaggct tgtttatctc tattacaagc ggttattttt
     3181 gctttatttt ttgcaatttt ctattgcttt ttcaagccaa aaataacgag tcattatcgc
     3241 attttaaccc tgaaaaaggc aaggtaattg ttgtatggta gctgttattt gttgtggaga
     3301 tgaatataat gaaaatgaat ggggtgataa ggaaaaataa tgagatttag tgtgagtgct
     3361 ggagataagc gtgaaatcca agcggttaaa tttgcaaaaa aagagctaaa tttaaccgct
     3421 tgtaaagcga atattatgct tttgccgtca tttttttcac tgcttcttca gtggtccaga
     3481 ggtcatttgc gataatacgg tagttggtta atgctgcaag gtagccgtca ccttcaggaa
     3541 tacgaggccc tgcggttgca tctttcacca ctgttacttc aaaaccttgt tcgattaatt
     3601 cacgcaagtg tgaatcaata cataagttgg cagccatacc ggctaaaatt acttggtcga
     3661 ttttacgttt gcgtaattgc aacactaagt cattggtttc cggtccaaag attttgtgtg
     3721 gagaggtaat cactgttttg ccatcaaaaa tgtatggttt gtactcaggc ataaagtctg
     3781 cacctgaatt ttcaaagcct tccatggtgt attgaccttt tctcgcaaac attccgctgt
     3841 cgtgcataaa gtgttcaccc ggtgaaccaa attcccattt gtgatcagtc gggtaatagt
     3901 agtgtggcga aacaaaggtt ggcatatcga ctgcttttgc tgctttaaat aagctttcaa
     3961 tgttagctac agtgttgttt tctttaattg agtcacctaa cacgccccac atcacgcctt
     4021 tcgggcctag gaagtcaatt tgtggatcaa ctaccactaa tgccacacgt tttggatcaa
     4081 tggtcattcc atggcgtttt aaaccgtatt gttccggctc ggcataaggt gatttttctg
     4141 cactggcttg ttttgcattt gctgttgatg caatacctaa tgcacctaat acactggctg
     4201 ccattgcaat acgagagccg tggttaaggg cttgacggcg ttcttggttg atttcttcaa
     4261 attgttcctg tgacatcatt ttatcctcat taatagatat acaaattgta atgataactt
     4321 gcgttaccga acgggttgct attatagtga tcgcatttct cctgttttaa ccgttcgtct
     4381 caattcttta cccaaggagc ttaatgtgga tagtttgact catcttttta cccaatttca
     4441 gtttcgcacg gatttatttc atctaggtca gttatgccaa atcgggagtt ttaatgaaga
     4501 gaataaaggc tatttacatt tcgttcgtca gggacgtttt attttaaatc aggctgccga
     4561 acgcccgatt acgattgaac gcccagcgat tattttttca ccaaccaata ttttgcatag
     4621 tttgcacccg ttagatgata gtgggttaga tatattttgt attaattttg attttggcaa
     4681 agggattcgc aacccattaa ctcatacctt gcacaatatt gtgatcttag agttggcaca
     4741 gtatccggaa ttgggggtat tagccgatca aatttttagt gaagtgaatc agaaagggtg
     4801 tggttatcag gcaatgattc atcatttatg tgcctatttt accttgaaag ttgcccgcca
     4861 atgtttggaa aaagggtata ttcaaacagg tttgttaaaa ggcttggcag ataagcaact
     4921 ggggaaagtg ctgttagcta ttcacaatgc accagagcga gattggaatg tagaggagtt
     4981 ggcaaatgtg gcattaatgt ctcgctcacg ctttgctgct tattttaaac aaacgatggg
     5041 ggtttcaccg atggattatc tcactaactg gcgactagcg gttgcccagc atttattaca
     5101 aaaagggata ccagtagcat tggtggcaga gcaagtcggt tatagccata atgctgctct
     5161 cagccgtgtc tttatgcggg aattagggct ttcgcctacc gagtggctgg ctcaaaataa
     5221 agagtcgtaa cctgatgaga aacaaatagc cgaatggtaa aaatgtcaaa aaatttaacc
     5281 gcttgttatg ttgcacaagc ggttaaatgt tgccttaaat gccaagagaa tcccaaatct
     5341 catcaatgcg tttcaccaca ttgtcatctt tttcgatggg tgttccccat tctcgggtgg
     5401 tttcacccgc ccatttattg gtggcatcaa tccccatttt tgagcctaac cctgcaattg
     5461 gcgaggcaaa atcgagataa tctatcggcg tgtgttcaat cagtgtcgta tctcgagccg
     5521 ggtcacaacg ggtggtgatc gcccaaatta catctttcca atctcgagcg ttcacatcgt
     5581 catcgcacac aatcacaaac ttggtgtaca taaattgacg caggaacgac caaactccca
     5641 tcatcacccg tttagcgtgt ccggcatatt gttttttcat cgtcactacc gctaaacggt
     5701 aagaacagcc ttcaggtggc aagtagaaat cgacaatttc cggaaactgt ttttgcaaaa
     5761 tcggaataaa gacttcattc aatgcttcac ctaatactgc cggttcatca ggtggtctgc
     5821 cagtgtaggt agaatgataa atcgcatctt ttcgcatcgt aatatgggta acggtaaata
     5881 ccggaaaata ctcttgctcg ttgtagtagc cggtgtggtc gccataaggg ccttccaatg
     5941 cggtttcttc cagatcgata tagccctcca gcacaatttc gctacttgcc ggcacttcta
     6001 actcattgga aatagatttg accacttcgg ttttatgccc tcgcaacagc cctgcgaacg
     6061 catattccga aagcgtatcc ggcacagggg tgactgccgc taaaatagtg gctggatctg
     6121 caccaatcgc aaccgacacc agaaacggtt tacccgggtt ttcggctttc cactcttgga
     6181 agtctaacgc accgccacgg tgggaaagcc aacgcataat cagtttgttt ttgccgataa
     6241 gttgctgacg ataaatgcct aaattctggc gttttttgta aggcccttgc gtaatcgtca
     6301 gcccccaagt gaccagtgga gcgacatcgc ccggccaaca gtgcataatc ggtaatttgt
     6361 agagatcaac ctcatcaccc gaaatcacca cttgctgaca atcggctttg ctcagtacct
     6421 taggcggcat atttaaaatt tgcttccatt gtggcagttg cccgatcaaa tctttaaacc
     6481 ctttcggtgg ttcaggctct tttaaaaagg caagtaactt gcccagctca cgcagagctt
     6541 tggtatcttc ttgccccatt cccagagcta cccgtttggg cgtgccgaat aaattacaca
     6601 acaccgacat ttgatagcct ttcgggtttt caaacagtaa tgccggcccg cctttgcgta
     6661 aggtgcggtc ggcgatttct gtcatttcta aataaggatc gatttcttgc tgaatacgga
     6721 ctaattcacc ctgagactca aggaaatcga gaaattcacg gagattttta tacttcatag
     6781 cggtcatatt tttcgatttt tttgcaagta tatagaaaaa cgtaaatgaa aaatacccat
     6841 aaaaaattta tagaaatttt gttttatttt gttagaatag ctcggttcaa attaaatgcc
     6901 ataaggggaa ttttatgttt aaaagaacat taatcggtct agccgtgtta ggtgcaagtg
     6961 ttgctcaagc ggatgtatta accagtatta agcctttagg ctttattgcc agtgccatta
     7021 ccgaaggggt aactgaaact aaagtattat taccggtaac tgcctctcca cacgattaca
     7081 gcttaaaacc ttcagatgtg actcaattac aatctgctca attagtggtt tgggtgggtg
     7141 acggcttgga aggcttttta gaaaaaagta ttgaaaaact gccgagagaa aaagtattaa
     7201 cccttgaaga tgttgatgcg attgatgata ttgtcgataa agcaagcaaa aaagaagata
     7261 aagacgatga tcacgatcat aaacatgaac ataagcacga acacaagcac gagcataaac
     7321 acgaagggca taatcacgaa ggacataacc acgatcatca cggacatagc cacgatgaag
     7381 attggcatat ttggctctca agcgaggcaa gtgagctggc agcagagcaa attgcggcac
     7441 gtttaagcga acaaatgcct gaacacaaag cgaaaattgc ggaaaatctt gcaaaattta
     7501 aagcaaatct aaccgctaaa aaagccgaaa ttgcgaaaca attagagccg gtaaaagcca
     7561 aaggttacta cacattccac gatgcttacg gctattttga aagagaatat ggtttaaatt
     7621 cattgggttc atttaccatt aacccaactg ttgccccggg tgcaaaaaca cttacagcaa
     7681 tcaagaaaaa cattgcgaaa aacaaagcac aatgtttatt tgccgagccg caatttacgc
     7741 cgaaagtaat tgaaacctta gcaaaagaaa ctaaagtaaa tgttggccaa ttagacccac
     7801 tcggtgcaaa agttcagtta agtgcgactg cttaccctga atttttacaa gttttggctg
     7861 acgaatttag ccaatgttta acaaaataag ttaaaaatcc cccatttaaa tataaatggg
     7921 ggattttgcg attacatcac ttaagctttt tttaagaatt ctgatttcaa cataatttta
     7981 ccgcaatcga cattatggtc accttgagct aaacggattc ctttgaattt tgtacctttt
     8041 ttcagtactt cagacgagcc ttttaatttt aaatctttga ttaataatac atcgtccccg
     8101 tcagataata aattgccatt actgtctttt acaattagca catcatcatc ttgatccgtt
     8161 tcttcgcccg tccattcata cgcacaatcc gggcaaacaa attgaattga gtcgtggtaa
     8221 gtattttcgc ttttacattc agggcaggtt gggtaaatca ttttaattcc tcaaattaat
     8281 aaagaaccag attatatacc gaaatccagc aaaacgctaa gcggtcatat tttgccgatt
     8341 ttttaccaat cgagctaaaa gtcagttaga atagccttaa ttacacaaaa aggaggatac
     8401 tatgatagtc gatccgaatt atcagcaaga attagaaaaa tacgaaaatc cggtaccaag
     8461 ccgagagttt attttaaata caattcgtga acataacgcc ccaatgagcc gtgatgaatt
     8521 attgtcggct tttcatattg acgatgaaga acgaatggaa ggcattcgcc gccgtctgcg
     8581 tgcaatggaa aatgacggtg aattggtgtt caccaaaggc aaacgctatg cgttgccgga
     8641 aaaaatggat ttaatcaaag gtacagttat cggccatcgt gacggttacg gttttttgca
     8701 agtggaagga cacgaaaaag gggcgactaa aagcgaagat tggtttattc ccaacgccca
     8761 aatggtaaag gtaatgcacg gtgactttgt gctggcacaa ccaaacggca cagaccgcag
     8821 aggtcggaaa gaagtccgca ttgtgcgagt gttggaggcc cgtaagaaac agattgtcgg
     8881 gcgttttttc cttgaaagcg gtatcggttt tgttgtgcct gatgacagcc gcgttaacca
     8941 agatattctc attccggatg aacaccgtat gggggcgaga atggggcaag ttgtggtggt
     9001 ggaattacag gaaagaaaag cgagttttag ccgcccggtg ggaatcatta ctgaaattct
     9061 aggcgataat ttagcgccgg gaatggaaat tgaaattgcc ctacgcaatc acgatattcc
     9121 gcacatttgg ccggaagggg tagaaaaaca aatccgccaa tttagtagcg aagaagtgcc
     9181 ggaagaagcc aagcaagggc gggtggattt gcgagatctg ccactgatta ccattgacgg
     9241 cgaagatgcc cgagatttcg atgatgctgt atttgccaaa aaagagggca acggctggcg
     9301 gttatgggtg gcgattgccg atgtaagtta ttatgttcgc ccaaaaaccg cattagattt
     9361 ggaagcccta aatcgtggta actcggtcta tttcccgaat cgggttgtgc caatgttgcc
     9421 ggaagtgctt tccaatggct tgtgttcact caatccacag gttgacaggc tctgcttggt
     9481 ggcggaaatg tcggtttcca gcaaagggga attacaagat tatcaattct acgaagcggt
     9541 aatgaactct cacgcccgtt taacttacac taaagtgtgg aaaatgttgg aaggtgatga
     9601 agccttgcgt gaacgctatg ctccattagt gccgcatatt gaagaactct atgcaatgtt
     9661 ccaaacctta atgaaagctc gccataaacg aggggcgatt gaatttgaaa ccattgaaaa
     9721 tcaatttatt ttcaatccgc aagggcgaat tgagcgaatt gagccgttaa tccgcaacga
     9781 tgctcataaa ttgattgaag aatgtatgat tttagccaat attgctgccg ctcgttttgt
     9841 ggaaaaagcg aacgaaccgg cgttatatcg gattcacgat aagccgagtg aagaaaaatt
     9901 gctcagtttt aaatcctttg tgcgtgagtg tggcttaacc tgggacgtag gcttagatcc
     9961 aaccccaaaa gattacggtg tactactgga gcaaattgcc gaacgtgccg ataaagagtt
    10021 gattcaaacg atgttgctcc gctcgctgaa acaagcagtg tatgcggcgg ataatatcgg
    10081 gcatttcggc ttggcattaa ccgaatatgc tcattttacc tcgccgattc gccgttaccc
    10141 ggatttactg ttacaccgtg caattaaata tctgatcgaa aaaggcaaag gcaacactcg
    10201 ctactatacc gatggcggtg gttatcacta caagttagat gatatggacg aattcggcga
    10261 taaatgttct gccaccgaac gccgagctga tgaggcaacc cgagaagtgg cagattggct
    10321 gaaatgcgaa tttatgcaag atcatcttgg cgaagagttt gatggcgtga tttcgagtgt
    10381 taccggcttt ggcttatttg taaaactcaa tgagctttta attgacggat tggttcatat
    10441 ttcaacgctg gataatgatt attaccacta tgatgcggac cgacaacgtt taatcggtgg
    10501 cagtgggatc atctatcgtt taggcgatgc agttagagta aaagtaatca atgtgaattt
    10561 agacgagaaa aaaattgatt ttgagttact caccggtaat aagaaaaccg gcaaaaccgc
    10621 caagaaaaaa gcaaaagcga cagaggttcc tgttttcaaa gagcctaaaa cggtaaaaaa
    10681 agccaaaaaa gtcgctgaga aaaaaccggc aaaatctgca aaaaaacgga caaaaacgac
    10741 cgctagtaaa tcaaggaaaa aatagcaaat ggattacaaa aaacaaattt tattcagcct
    10801 tttatttgct ttagtaattg caataataag ctattttttg tttacctata tcccgtttga
    10861 ttgtagcgat ctgagcaaac tcaacgattc acaacgttta gagtgccgaa ctgccgaatt
    10921 tctgggcgtt tattactggc tgccgcacac taccacgctg tttttcggct cggtgttatt
    10981 atattgggcg gtttatggca tgatttcatt agtgcaagca gtgctcaaac gctaacaagc
    11041 agttttgttt ttgcaaaaag ttgcaaatct catctgaata atccttccaa gatcaaaggt
    11101 ctatcaaaaa agatagacct ttattgtgta aataaactaa aactccttga tagatgtatc
    11161 acatatctat aaaattccag caattaagtg ggaaaaagtg gtaaattgtg gaacttttcc
    11221 ttaaatttaa cctcatttaa aaaggtaaat cagtaaaatg tttcgtggtg caagttcaat
    11281 cagcattgat agcaaagggc ggatcgctat tccgacccgt tatcgtgccg agctattgga
    11341 aaaacatcac gggatcttag tttgcactgt ggatatccgc cagccttgtt tgttgcttta
    11401 cccacttcac gaatgggaaa tggttgaaca gaaattgttg gcactatcta attttgaccc
    11461 tgttcaacgc cgtattcagc gagtaatgca aggctttgct accgaatgtg aaatggattc
    11521 ggcagggcga atactgctta gccctacact tcgccaacac gcacaattag agcaacaaat
    11581 tatgctggtt ggacaattaa acaaatttga aatttggcaa gataaacaat ggcaagcaca
    11641 aattgccgaa gatcttgctt ttggcagttc ggcagaaatt ttagactgcg aagcattgaa
    11701 aaacctgtct ctctagtaat tggaaattga gatgagcgag aacccaaaac atattacagt
    11761 attattgcac gaagcggttg acggcttggc aattaagcct aacggtatct atattgacgg
    11821 tactttcggg cgtggcgggc attctcgctt gattttatcc aaactgggcg aaaatgggcg
    11881 attaatcgca actgatcgtg acccacgagc gatagccgaa gctcaaacga ttagtgaccc
    11941 tcgttttcaa attgaacata cggcattttc tgccattccc gaactgtgtg aacgaatggg
    12001 gttggtgggc aaaattgacg gtattttatt ggatttaggt gtgtcttccc cgcagcttga
    12061 cgaagccgaa cgaggcttta gttttatgcg tgacgggccg cttgatatgc gaatggatac
    12121 caccaaaggc ttatcggcag ccgagtggct tgcccaagtt tcggcagaag atttagcttg
    12181 ggtattgaaa gaatttggcg aagagcgttt tgccaaacgg attgcacaag cggtcgtttc
    12241 ttacaataaa tctgcaaatg aaaaaatcag ccgcactttg cagcttgcgc aaattattgc
    12301 ggatgcggtg ccgtttaaag ataagcacaa acaccctgca acccgttcgt tccaagcaat
    12361 tcgcattttt atcaatggtg aattagacga gctggaaaaa gctttaaatt ccgcattaac
    12421 ggtgttagcc cctgaagggc gtttgtcgat tatcagcttt cattctctag aagaccgaat
    12481 ggtgaaacag tttgtgcgta agcatagtaa aggaatggac gtaccccgtg gtttaccgat
    12541 tttagaagca gagctaacca aaaatattcc gctgaaaacc attggcaaag cgattatgcc
    12601 gagcgaagcc gaaattgaag caaatgcccg ctcacgcagt gcggtattgc ggattgccga
    12661 aaaacgctaa cagtaaatag ggaagaatat gtcgaacgaa cgttatccgt tacatcaaat
    12721 catcattgat gatatcttta gccataacaa agtggctctt ttattgttga ttggtgtagt
    12781 gatttcagca gtggctacga tttggataac ccaccagact cgtttatcgg tgagcgaaca
    12841 agggcaactg attcaagcaa accaaaagtt ggaaagccaa tatactaatc tgcaattaga
    12901 agaaaacagc agaagcagtc gggcaagagt tgatgccgcc gctaaatcgt tcggattaca
    12961 gccgattaaa aaagagcaag aaattatttt agttgaataa aaaatggtta aatcagttaa
    13021 attaaaacgt aagcaagaag cccctgtcgc aaaagcagag cctcaacaag cagaaggtaa
    13081 aaaaacaccg agttttttac ctactcgctt tagagtggtt gtgtttttta tgctattaat
    13141 ggttggtggc ttactagcac aaaccgccta tattcagctt tttgactctg aaagattaat
    13201 taaagaggct aataaccgct cacttcgtac taaagaactc caatttaccc gtggacgtat
    13261 tttagatcgt aacgggcgtt ttctctctat tagtgtgcca atgtactcat taacacttga
    13321 cccgagagaa tactttgata ctaagctgcg tcgttctaac gaaagctggc gtgccttagc
    13381 gattgaaatg gaaacatcaa aaggtaaaat tgaggcaaac gttaataaat ttattgaaaa
    13441 gaaaaatttt tcaaaagaaa aagtagattt tgatccacgt tctattttaa atactaaaag
    13501 tgaaggttat tggactcagc ttgcccaagt tacaggtttg gattacaaca gtttaataga
    13561 aaaagtacgg aataacccta gctcggtttt tttacgttta gataacgaaa atttagtaag
    13621 agaacgccag aaattccaag cgttaagcaa agaaatcggc cgttcctata atgacataat
    13681 ggacgagctt tatagcaaaa accgccagcg attcctttac ttatcccgtc atcaatctga
    13741 agcgatcagc gaatatgcaa aagaactaga tattgatgca ttagtgatta aagtcgaatc
    13801 acgccgtttt tacccgctgg cagaagaatc ttcacagttg atcggtttta ctgataaaga
    13861 tgatatgcac ggttcggaag ggctggagcg tagttttgat tccttactta tcggcaaaaa
    13921 cggtaaacaa attatccgaa aagatgccaa aggcaatatt gtggaaaata tccgttcgga
    13981 gaaacagtac gatccgcaag atgttatgtt aagcattgat gaagaattgc agtctatggt
    14041 gtacggtgaa atcaaaaaag cggtacagga aaataatgcg gaatcaggca ctgcggtttt
    14101 ggttgatgtg caaaccggcg aaattttggc aatggcaaat gccccttcat tcaacccaaa
    14161 caaacgggat agttttaaac ctgaattaat gcgtaaccgt gctattaccg acacttttga
    14221 accgggttca acggtgaaac cttttaccgt attaaccgca ttacaaaatg gggtaactta
    14281 tcgtgatgaa gttattggca ctcgcccatt tgtcgtcaat ggacatacaa ttaaagacgt
    14341 tgctccaagg gacagcctta cgcttaccgg catcttgcaa aaatccagta acatcggggt
    14401 cagtaaatta gccctcagaa tgccggcaaa tgcgttagtg gatacttaca cgaaagtcgg
    14461 tttcggtaaa gacaccggtt taggcttggg tgaacaacga ggtacgaacg gtgatcgcaa
    14521 acgttgggcg gatattgaac gtgctaccct ttcctatgga tacggtttaa atgtgacccc
    14581 attacaactt gcccgtgctt atgcgacttt aggaagtttc gggctgtatc gcccactttc
    14641 gattaccaag gtcgatccgc cggtgattgg cgaacgggtg ctaccggaaa aaaccacccg
    14701 agatgtcgtg catatgatgg aaagcgttgc ggctaaaggc gaaggcggac accgtgcagc
    14761 ggtggacggc taccgtgtag ccatcaaaac cggtacagca cgcaaattag aaaaaggtaa
    14821 atatgttgaa aaatatattg cttataccgc agggcttgcc ccggcaagcg atcctcgctt
    14881 tgcattagtg gtattaatca acgaacctaa agcaggcaac tattatggtg gctttgtttc
    14941 tgcaccactg ttctcacgca ttatgggtta caccttaaaa caacgtaata ttaagccgga
    15001 taacttaacg gaaaataccc aaagtgccgt gcgtgaaatt aaattagaac gccctatcaa
    15061 ttaaggttag ctatgaaacg tttactccct tttctgacag aacttgatgc ttgggtggaa
    15121 gaactcaccg agctaaaaca aatgacactt gatagtcgcc aagttcaagc cggtgatctc
    15181 tttgttgctt taaaaggaca tcaagtcgat ggcagaaaat ttattccgaa tgcgatagaa
    15241 caaggagcag caattatttt ggctgaggct gatgaagatc aaccggaaat tgagcttgat
    15301 ccgaaatttg caaaatttaa cctagatcgc accgcttgtt gcaaggttgt ctctgttcca
    15361 cgtctgccaa aattgctctc tgaaattgca ggagctcttt accataaccc gtcacacaag
    15421 ctgactttag ccggcattac cggcacgaac ggtaaaacga ctactgctca attattagca
    15481 caatggcata atttattggg tgggaaatcg gcagtaatgg gtactatcgg caacggtttg
    15541 tacggacaag tgcaagaagc ggtaaatacc accggctctg ccattgaaat tcagcataat
    15601 cttgcgaact ttgttgaact tggagcggat ttttgtgcga tggaagtcag ctctcacggt
    15661 ttggctcaat ttagagccga agccttggat tttgatttag cgatctttac gaatttaagc
    15721 cgagatcacc ttgattacca caacacgatg gaagagtatg cccaagccaa attccgtctc
    15781 ttcaacgaat taagcaccaa agctcaagta attaacgccg atgatgaaat cgggcgtgaa
    15841 tggctgacac aattaccaaa tgcagttgcg gtaagcaccg atccgaagtt tgcaggcaac
    15901 caccaatttg tcaaagccac tgcggtaaaa tttaccctac aaggggcaag tattgcgttt
    15961 gaatcaagtt ggggcaatgg cgagttacat agccgtttaa tcggggcttt caatgtaaat
    16021 aatctgctta ccgcctttgc cggactttta gctcttggct ttgatattca agaactggtt
    16081 aaaaccgccc cgaatttagt tggcgtagca ggtcgaatgg aatgcattac cgctccaaat
    16141 aagccaatgg taattgtgga ctatgctcac acccctgatg ctcttgaaaa agccctacaa
    16201 gcggctcgtt tacattgcga aggcgaactt tggtgcattt tcggctgtgg tggagaccgt
    16261 gacgccggca aacgcccatt aatggcgacg attgccgaga aattaaccga taaagtgatt
    16321 gctacagacg acaaccctcg caccgaagat aatcaaaaga ttatggcaga tattgtgaaa
    16381 ggttttagta aaccccaaca aattatccac aatcgtgaag aggcaattaa aaccgccatt
    16441 gagcaagcta aagccacaga tgtgattcta atcgcaggca aaggtcacga agattatcag
    16501 attattggca cagagaagtt acatttttcg gatcaggaaa cggcaaggaa atatctgttt
    16561 taatgtcact ttctttgcac aagcaaagaa aagtgacagt gacaaataca aaatgctcgt
    16621 aattaatttt ataaagaaaa agataaatca atgataaaac taaccaccca acaaatcgct
    16681 gaaatcctaa atgccaaatt gatcggcaat ggcgaaactg tggtagaaac caccagcacc
    16741 gacacccgac aagcggttga aaacggctta ttttttgcat taaagggcga gaactttgat
    16801 gctcatcact atcttcaaaa tgcagtcgca caaggttgtg tagcggttgt ggttgagcgt
    16861 gagtgtgaaa ttgatgtacc acaacttgtg gtggcagata cccgtttggc attgggggaa
    16921 ttggcgaaat ggttaaaagc taagctgaac ccgaaaacgg tagcgatgac aggttcttcc
    16981 ggcaaaacaa ccgtcaaaga gatgacggca aaaattctgc aaaaaatgac cgcttgcgaa
    17041 caggaagtgc tttatacttt tggtaactta aacaatgact taggtgtacc gatgaccttg
    17101 cttcgcttaa ccgaacagca taaatatgcg gtcattgagc tgggggcaaa tcatatcggt
    17161 gaaatcgctt ataccaccgc tatcgctcag cctgatgcct gcttggtgaa taatgtggct
    17221 gcggcgcatt tagaggggtt tggttcatta gcaggcgtgg cacaagccaa gggcgaaatt
    17281 tatcgtggct tgaaagaaaa tggcaaagcg attgtcaatt tagcttttta ttatccgcaa
    17341 tggcaaaaag agattggctc acgggaaatg caatctttct cttatatcgg gtcagattcg
    17401 gattcttacg cagattactg ggcggaagat gtagaactgc atttaagcgg ttcacgcttt
    17461 acgctacata ccccacaggg cgaaattgct attgatctgc cttatttggg caaacataac
    17521 gtgagcaatg ccttagcggc aacttcgctg gcgatgtctg ttggggcaac gctagaagca
    17581 gttaaggcag ggcttgagca gcgttcaatg gtaaaagggc gtttgtatcc ggtgcaaatt
    17641 aacgaccact gtttgtttat tgatgacact tacaacgcca atgtggattc aatgaaatcg
    17701 gcgatttctg ttttggaaaa ttatccggct tatcgtattt ttgcagtggg tgatatggcg
    17761 gaattgggtg aagaaagtag caaatgccac caagaagtag cggattttgc caaaaatgca
    17821 gcattagatt taatcgtgag ctttggaaaa gaaagtgcgg taatcagcca acaggcagaa
    17881 catcatttta ccgataaaca gcaaatggtt gatttcctac tgccgattat tttgcaaaaa
    17941 atcgaacaaa aacaaccgct tgtattattg gcaaaaggct ctcgtagcca gaaaatggaa
    18001 accgtgattt ccgaattgtg taaaccgttt aacctaaatt tctaattatt tttaaggact
    18061 aagcaatgtt agtttggctt gcagaactat tggttcaata taacacagcg tttaacgttg
    18121 tctcttatat tactttccgt gccattatgg cgttattaac tgcaatggga atcgggcttt
    18181 ggatcgggcc gaaagtaatt cgccgtttgc agatcttaaa atttggtcag gaagtgcgta
    18241 atgacggacc ggaaagccat ttcaaaaaac gtggtacgcc aacaatgggc ggagtgatga
    18301 ttctcgcagc gattggggtg agtacattgc tttgggcaga tctgcgtaat tcctatgttt
    18361 ggttcacgct gtttgtgcta tttggctacg gtgcagtggg ctttgttgat gattatcgta
    18421 agatctctcg taaaaatacc gatggtttga ttgcccgttg gaaatatttt tggttatctg
    18481 ctatcgcatt agtggcggtg tttggtatgt atgcggtggg taaagatacc gctgcaacac
    18541 aattagtcgt gccgtttttt aaagatgtga tgccgcaact cggtttattt tatattgtgt
    18601 tatcctattt cgtgattgtc ggcaccagta atgcggtaaa cctaacggac ggcttagacg
    18661 ggcttgcgat tgtgccaact attatggtcg catctgcctt tgcgttaatt gcgtgggcaa
    18721 ccggtaactt taattttgca caatatttac atattccttt tattccaaat gccggtgaat
    18781 tggttatttt atgtactgcg attgtgggag caggtttagg gttcttatgg tataacacct
    18841 accctgcaca ggtgtttatg ggcgatgttg gctcactttc gctcggcggt gcattaggcg
    18901 tgattgcagt attagttcgc caagaattat tattggtggt aatgggcggt gtatttgtgg
    18961 ttgaagccct ctcggtgatt ttacaagtag gttcatacaa attacgccaa aaacgtattt
    19021 tccgaatggc tccaattcac caccattttg aactcaaagg ctggtctgaa ccacgggtta
    19081 tcgtgcgttt ctggattatc acgctaatgc tggttttagt cggcttagta acattgaaat
    19141 tacgttaatt gattgaataa ggtgcattga gataatgtac cttatgttat ttttaaatta
    19201 cagaataaat tatgcaaaaa caatacgaaa acaaaaccgt tactattatc ggcttaggta
    19261 caacagggtt atcttgtgtg gatttcttta agaaaaaaaa tgccaatatc caagtgattg
    19321 acacccgagc aaacccagcc ggagcggata aattggacgt aactattccg ttgcataccg
    19381 gtgggttaaa cagtgaatgg ttgctgaaat ctgatcttat tgtaattagc ccaggtttag
    19441 ccgttgccac acctgaaatt cagcaagcga ttcaagctgg tgttgaaata gtcggtgatg
    19501 ttgaactgtt ttgtcgggaa gcgaaagctc cgattattgc gatcaccggt tcaaacggca
    19561 aaagtaccgt aacgacttta actaccgaaa tggcgaagca agcggggatt aaagtgggaa
    19621 tgggtggcaa catcggtgtg ccggtattaa gtttgctgga gggcgattat gacctttttg
    19681 tgctggagct atccagcttc cagcttgaga caacttattc actgaaagca aaagctgcca
    19741 ctgttttaaa tattagcgaa gatcatatgg atcgctacga ttcgattgcc gactatcgcc
    19801 aagcgaaatt gcggatttac gataatgcgg aaaatatcat cgtaaacggt gaagatgccc
    19861 aaacttaccc gatacaagcg gtcaaaaaag tgattcgttt tgcagaacaa aatgcggaat
    19921 atagcctacg caatcagatg ctttattcag gcgatgatgc aattatccaa actaaacaga
    19981 tgctgattag cggccgtcat aatgagatga atgcgttagc ggcgatggca ttagcagaag
    20041 cggcgaatgt tcctcgtgaa ggcattgtga aagctttgca aatttatggt ggtttgccac
    20101 accgtttcca agcggtggca accaatgatg gcgtgcgttg ggtcaatgat tccaaagcga
    20161 ccaatgtggg tagcacggta gcggcattaa acggcttgaa tattgccggt acactttatt
    20221 tacttttagg tggtgacggc aaggctgcgg atttttccga actcaaacca ttaattaata
    20281 agccaaatat tgtctgctat tgtttcgggc aagatggagc ggcattggca gaattaagcg
    20341 aacaaagtgt gttggttgaa acgatgcagc aagcaattga agcaatccgc ccacaactga
    20401 aaaaaggcga tatggtactg ctttctccgg cttgtgccag tttagatcag tttaaaaact
    20461 ttgagcaacg tggcgaggta tttatggcat tagcacaagg acagcacgca taatgttaga
    20521 aagaattaag tcggaatggg ataaatgggt cagattaacc ccttctaatt cactttatga
    20581 cagaactcta atttggctat ttttaggcct gctaattatc ggttttgtga tggtaacatc
    20641 tgcttcactc ccagttagca caaggttgaa taacgatcca ttttattttg cgattcgaga
    20701 cgggctgtat attattgctt caattatctt ttgttatgtt tttgtacaaa tcccaattga
    20761 aaaatgggaa aagcataact tggcactgtt ttttatttca atcggttttt taatcgccgt
    20821 actcattttc ggccgctcaa ttaatggtgc agtgcgttgg attccgttgg gtattctcaa
    20881 ctttcagccg gcagaattag ccaaattagc ggtcatttgc tattttgcca gtttctatgt
    20941 acgtaaatat gatgaaattc gtaaagaaaa agccagtttc tggcgaccgg cagtgatttt
    21001 attcttattc ggcttcttgc tgattttgca gccggattta ggtagtacct ttgtattatt
    21061 tgttctcact ttctcaatgc ttttcattgt gggggcaaaa attatgcaat ttatgttttt
    21121 aggcgtggtg ggaacggtgt tatttgcggt gttgatttta acctcggaat accgtttaaa
    21181 acgtgttacc tcctttatgg atccgtttgc tgatgcttat ggcgacggtt tccagctctc
    21241 gaactcacaa atggcatttg gacaaggtga attttgggga caaggtttag gtaattcggt
    21301 acaaaagcta gaatatttgc cagaggcaca tactgacttt gtaatggcgg ttattggtga
    21361 agaatttggt ttctttggta ttgcttgcat cgtactttta cttattctat taacggtgag
    21421 agccttaaaa atcagtaaag aatctttggt gctggaagag cgttttaaag gctatatggc
    21481 atttgggatt gcgatttggg ttttcctaca aggcttcgta aacttagggg tggcatcagg
    21541 tttgctgcca acgaagggct taaccttccc attagtgagt tatggtggtt cgagtttggt
    21601 aattatgtcg attgcgattg cagttttgct acgaattgac cacgaaaacc gtgccgagcg
    21661 aatcgggcac gcaaaaatta aaaccatcta gccgagcggt ctgcaacgtt acaatttgca
    21721 aaattttaca aaaatgagac cgcttgtatt agctggaaag cataaaaatt cagtaaaata
    21781 gcacgtttta gaataaaaaa gagttgaaaa tggcgaagaa attattagta atggcaggtg
    21841 gaacaggggg gcacgttttc cctgcaattg ccgtagctcg agagctacaa aaacaaggct
    21901 ggcaaatccg ctggctgggg acgaaagacc gaatggaagc cactcttgtg ccgaaacacg
    21961 gtattgagat tgattttatt gaaatctcag gcttgcgggg taaaggcgtt gcagcattgt
    22021 tgaaagcacc gtttgctatt tttaaagcgg tgatgcaagc ccgcaaaatc attaaaaatt
    22081 atcagcctga tgcggtgttg ggaatgggcg gttatgtgtc cggtccgggc ggtattgcgg
    22141 ctaaattatg tggcgtgccg gtgattttac acgagcaaaa cgcagttgcc ggcctaacca
    22201 atgtgtggct atccaaaatt gcccgcaagg tgttacaggc tttccctact gcttttaaag
    22261 aggccgaagt agtaggtaat cctgtacgtg aagatttatc tgctcttccg ctgccgaatg
    22321 aacgtttcgc caaacgaggc tacccgatta acattttagt aatgggtggc agccaagggg
    22381 caagagtgat caaccaaacc gtgccggaag tggcaaaagt gttgggtaat aatgtgttta
    22441 tcagccacca agtcggtaaa ggtaatttag ccggtatcga agaagtctat caacaaaccg
    22501 gcaatgggat tgcttccgag tttattgatg atatgaaagc cgcgtatgag tgggcagatt
    22561 tggtgatttg tcgttccggt gcgttaacgg tgtgtgaaat tgccgccgta ggtctgcccg
    22621 ctgtgtttgt gccgttccaa cataaagaca ggcaacaatt tttaaacgct aactatttgg
    22681 cggcagatgg agcagcagtg attatcgaac agagcgattt cactccaaag gcattacagc
    22741 aagccttaga acccttaatt gccaatcgcc aactcttatt ggatatgtca atgcgagcca
    22801 gagcaaaatc caccccaatg gcagcaaaac gtgtagcgga agtgattatt caagaagcga
    22861 attaacttac tacaagcggt cgtttttggt aaaaattttg caaaatgtaa ccgcttgcga
    22921 atagattaaa aggacaataa atgaaaaatt tccaagataa agtcaaaaaa ttagtgcctg
    22981 aaatgcgtcg tgtcagccaa attcatttta tcgggattgg cggagccggt atgagcggca
    23041 ttgcggaagt gttattaaac gagggatacc aaatttcagg ttcggatatt gcagacagag
    23101 cggttacaca acgtctatcc gcagccggag cgaaagtctt tatcggacac caagcagaaa
    23161 atattgctgg ggcgagtgtg gtggtggttt ctactgcgat tgatgaaacc aaccctgaaa
    23221 ttattgccgc tcgagaagct cgaattccgg tgattcgccg agcagaaatg cttgccgaaa
    23281 ttatgcgttt tcgccacggc attgcgattg caggcacaca cggtaaaacc actacaaccg
    23341 caatgatttc aatgatctac accgaagcaa aattagatcc gactttcgtg aatggcggtt
    23401 tggtaaaatc taccggcaaa aatgctcatt tgggggcgag ccgttattta attgccgaag
    23461 ccgatgagag tgatgcttct ttcttgcact tgcagccaat ggtgtcggta gtaaccaaca
    23521 ttgagcctga ccatatggac acctatggtg gcgattttga gaaaatgaaa gagacttacg
    23581 tacgattttt gcgtaactta cctttctacg gcttagcggt aatgtgtgcc gatgatgaaa
    23641 cggtaatgga aattgctcct cgtgtcggca gacaagtgat tacctatggc tttagcgaaa
    23701 atgcggatta ccgaattgaa gattaccagc aaaccggctt ccaagagcat tacaccgtag
    23761 tttgcccaag cggtgaacga attgatgtgc tgttgaatgt gccgggtatg cacaatgcct
    23821 taaatgcaac cgctgcgtta gcggtggcaa aagaagaagg gattggcaat gaggcgattt
    23881 tagctgcttt agctgatttc caaggggcag gacgccgctt tgaccaatta ggttcattta
    23941 ttcgcccgaa cggcaaaatt atgttagttg atgactacgg ccaccaccca actgaagttg
    24001 atgtaaccat tcaagccgct cgttcaggct gggaaggtaa acgtgttgtg atgattttcc
    24061 aaccgcaccg ctattctcgt acccgtgatt tattcgatga atttgtacaa gtactgtcta
    24121 aagtcgatgc cttgatttta ttggatgtgt atgctgccgg cgaagctccg attgtggggg
    24181 cagacagtaa agccttagcc cgttcaatcc gtaatttagg taaggtcgat ccgattttgg
    24241 tttcagatac cgatcaactt ggcgaggtat tagatcaaat tattcaagac ggtgatttaa
    24301 ttttggcaca aggtgcgggc agtgtaagcc gcatttctcg tggcttggct gagagctgga
    24361 aaaattaaat actctctccc cttgcgggag agagaccgaa aaatacttcg ttcagaagta
    24421 tttttcagag agagggtata tatttaaagg atttcaaatg agtattaaaa acgaaaaaat
    24481 tgccgtctta ttcggtggcg tttcgcaaga gcgtgaagtt tcactgaatt ctggtgcagc
    24541 ggtaacagct gcattaaaaa gtttaggcta taacgtagaa ggcattgata ctaaagaatt
    24601 cccgattgaa aaattaaaag aaaaagggat tcagcgtgta tttaatattt tacacggcgg
    24661 tattggcgaa aatggcgtac tgcaaggtgc gttagagcaa atgggtattc cttacaccgg
    24721 ttgtggcgta atggcatcag cagtgacgct ggataaattc cggactaaat tattatggaa
    24781 tgcagtaggt ttaccaacag cggatatggt ggtggttcag cgtggacaag cggtcgattt
    24841 tgaccaaatt attgcaaaat taggcttacc ggtatttgtg aagtcttcgt gtgaaggctc
    24901 aagtgtgggt gtattcaaag taaaaaccaa agaagaattg gcaccggcaa ttcacgaggc
    24961 attaaagttt gattccattg tgttggtgga agaattttta gccggtaatg aatattctgt
    25021 accggtatta gacggacaag ttctaccggc ggtacaagta attccggacg gcgaatttta
    25081 tgactaccac gcaaaatatg tgtcggataa cacccaatat gtagtaccgg cattaaatga
    25141 cgaacgccaa gcagaggtcg ccaaattagt gaaagcggct tacgatgtgg ttggctgtcg
    25201 aggttggagc cgtattgatg tgatggaaga tggcgaagga aaattccgct tggttgaagt
    25261 aaataccaac ccgggaatga ccagtcacag tattttccca aaatcagcag caacagttgg
    25321 tatttccttt gaaaaattag ttgaacgcgt attggagtta agtgcttaat gggcatcttc
    25381 cgcaagaaga acagcacaaa tgtgcgtttg aatgttctta atccaaaaac acctgtttct
    25441 ccacgtaaat ggctagtatt cattaagcca ttaattgtgt tattatgtac actatttgta
    25501 ttcggtgtgt atagtaattg gcaaaatata ctcgaaagtt gggataaaac accgattcgc
    25561 gcttatgcat taacacataa aacacaattt acaacaaatg cagatattcg tgaaactttg
    25621 tctaaagaac ctgctctaaa aggatatttc ggacaaaata ttcaagaggt caaagataag
    25681 cttttatcta taccttgggt taaagatgtg gctgttcgta aactttatcc cgaccgatta
    25741 agtattactt tgctagaaca taaacccgtt gcaatttgga atgatactaa ctttgtctca
    25801 gaacaaggaa ccgtctttag tttaccgaaa gacagaatcg ataaaaatgg acttccatta
    25861 ctctatggtc cagatacgga aggaaaaagt gtattagagg catggaataa aatccgagct
    25921 gatctaaatg cccgcaactt ggatttaacc tccgtttctg tagataatcg aggttcttgg
    25981 acaattaggc tttctaatca agtagaatta agattaggaa gaggggaatg gacttctaaa
    26041 attgatcgtt ttgtaacaat ctttcctgaa attcatatac ctgaaggtaa tagattagct
    26101 tatgttgatt tacgttatcc acatggcgcg gcggttggat ttagtcctat aaaaaattaa
    26161 actcagtgaa cacaaaaaca ttatgacaaa agcagtagaa tcaaaaatta tcgttggtct
    26221 tgacattggg acatcaaagg ttgttgccgt tgttggtgaa gtattacctg atggtgtaat
    26281 taatgttatg ggagctggcg tttgcccttc taaaggagtc gatcgaggtg gcgttattga
    26341 tcttgacgct gccgttaatt ctattcaaag agccattgag caggcggaat ctattgccga
    26401 ttgtcaaatt atgggtgtaa cactggcaat ctcaggtcag catattgttg gtttaaatga
    26461 agcaggatca tcaacattat ctggaggtat tgttacccaa gataatattg aaagtgctgt
    26521 ggatatggca aaggcaatta aattacaaga tggcttagaa acattacaca tgattccaca
    26581 agaatatcac gtggatagat tgcctgcgac taaaaatcca attggcttat cgggtatgcg
    26641 tttacaagtt caagcacact taattgcttg ccatcaagct tggttaaaaa acttaaaaaa
    26701 tgcggttgaa catgctaaat taaaagtcga tcaagttgta ttttcaggtc ttgcctctag
    26761 ctattctgtt ttaactgaag atgaaaaaga gctgggtgtt tgcttaattg atattggtgg
    26821 cggtacgatg gatgtactag tttataccga tggagcatta agatacagta aagtcattcc
    26881 atttgcaggt aataacatta ctgattattt ggctcgtgta tttacgacat ctcgccctga
    26941 agctgaaagc cttaaagtag gttatggtag cgcaatttcg cctccaaccc ataattcaga
    27001 taaaaaaatt gaggtggcag gactaggtgg acggatggca agaaccttta cccgagctca
    27061 ggtggcaacg gttacctcac aatgttataa cgatttgcta aaagttgttg aagaggaatt
    27121 aacccaatta cgccatgaac ttttccaaaa aggaataaaa caagagttaa ttgccggtgt
    27181 agtcattact ggtggtggct cacaaattga agatattgta gaatgtgcta aatcaatttt
    27241 tggttcacaa gttcgtgttg gatatccact caatattact ggcttaaccg attatgtgaa
    27301 taaaccacaa tatgcaacag cattagggct tttacaatat agccactata attatactga
    27361 acaaggtaca agtacagatt ctaatgaaga tatctttgct cctattggtc gtggaaccaa
    27421 gaaaatcatt gacttcttca aatcaaaatt ttaatagtgt taaaataaac acgcagggag
    27481 ataaaaatgt ttgaacctat tattgaacaa acgcaagatg ctatcattaa ggtagtgggt
    27541 gttggtggcg gtggcggtaa tgccgtagac agaatgtctc gctctgccga tgatattaaa
    27601 ggtgttgaat tctttgatgt aaatactgat gctcaagttt taagaaaaag aaccacacgc
    27661 caaactatcc aaattggagc aagtacaact aaaggtttag gtgcaggtgc tgatccaatg
    27721 gtaggaaaac aagctgccga agaagatcgt gaggctatcg ctaatgcatt aaaaggtgca
    27781 aatatgacct ttattgctgt tggtatgggt ggaggtaccg gtaccggagc agcccctgtt
    27841 gttgctcaaa ttgccaaaga gcaaggctca ctaactgtag gcgttgtaac aaaacctttc
    27901 cgcttcgaag ggcctcgccg tatgcgcttt gcagaccaag gcattaaaga gctttctcaa
    27961 tatgttgatt ctcttattat aattcctaat gataaattac gtggtttagg taagcaaaca
    28021 acagcagtag atgcctttgc cgcagcaaat gatgttttaa gtaactgtgt gcttggtatt
    28081 accaatatga ttaccagttc gggcggttct accggtgcag atattaacgt ggacttcgcg
    28141 gatgttcgta cagtaatgtc tggtaaggga cacgctatga ttggtacagg ttttgctgaa
    28201 ggtgaagttg gtgaaggacg agcggaaaaa gcaatgaatg atgccatttc tagtccatta
    28261 cttgaaaatg tggatatttc aggtgcaagt ggtatgctca tcaacattag tgctgggaca
    28321 gatttcttat tagaagaagt ttatgctatg atggacttaa tttatggctt tgcaacagaa
    28381 gatgccgcaa ttgtatttgg ttgtaactat taccctgaaa tggatggaaa agtgagtgtc
    28441 actcttgtcg caacaggaat aggccaacct gaggaagctc tacatatgcc acataaggct
    28501 cagccagtat atgcacagca aggaaatcat gttcaagcta cacagcctac tcagccaaac
    28561 catgcggtac aacaaccaaa ctttactcaa ccgtcaagtt atggtcagcc aaatcaattt
    28621 agtggtcaac cacaaccact acaacaacct gtggctccaa aaccacaatc ggttgataca
    28681 gcacaaattt ttaacccaaa ttcaattccg ggttttatga gaaatagtca ataacttcca
    28741 ctcatttcgg aagtaaggac aaaatatgac taaacaaaga acattaaaac aagcagcaaa
    28801 agtaacaggt attggcttac atagcggcaa aaaagtaacc ttaacgttac gccctgcccc
    28861 agctaataca ggagttattt atgcccgtac cgatttagag cctgtcgttt atttccctgc
    28921 aagcgcagac tctattcgtg atacacaatt atgcacatgt atggtaaatg atgatggtgt
    28981 tcgtatttca acagtagaac atttaaacgc agcaatgtca gcattaggct tagataatgt
    29041 tattgttgaa gtagatgcac ctgaaattcc gattatggac ggtagttcaa gcccatttat
    29101 ctatctttta ttagatgccg gcatcgaaga acaagatgca ccgaagaaat ttattcgcat
    29161 taaagaaaca gtacgcgttg aagaaggcga taaatgggcc gaattaaaac catacaataa
    29221 aggttttaaa ttgaacttca ctattgactt tgagcaccca ttaatcccca aaaatgtagg
    29281 gagctattcc attgattttt ctgctgagaa ctttattcag caactaagtc gagcgagaac
    29341 ctttacattt atgaaggatg tagaatactt gcaatctatt ggtcttgcat taggtggaag
    29401 ccttgataat gctattgtat tagatgatta ccgaattcta aatgaagacg gtttacgatt
    29461 taaagatgaa ctggttcgcc ataaaatgct ggattcgata ggtgacttat atatgtgtgg
    29521 ttataatatc ctaggtgaat ttagtgcata taaatcaggt catggcgtta ataataaatt
    29581 attaagagct gtattggaaa ataaaaacgc ttgggagttt gttacctttg aagataaagc
    29641 agaagtccca caaggttacc aagtacaaca agaaattttt atttagtcta aatggcttaa
    29701 aagacgaaca gaaatgttcg tcttttattt tgcaaatttt ttctaatatt taaccgcttg
    29761 caacccgcct cattagcgat tttttcgccc ttatctattt actgtttaaa aaaacagttg
    29821 tactatatac ccatacagtt aatcttgtgt ggtgtattat gcttactcaa cttactatta
    29881 ataattttgc gattgttcgc catcttattc ttgaattaaa tgaaggtatg tctgttatta
    29941 ccggtgaaac cggtgccggg aaatcaatcg ctattgatgc cttagggcta tgcttgggtt
    30001 atcgttctga aagtgggatg attagaaacg gttcggacaa agcagatata agtgcaacct
    30061 ttactatgca gccacatagc cctgcttatt tatggctaca agagcatgaa ctgcttgatg
    30121 aagataatcc tcaagagtgt atcttacgcc gtatgattaa tattgatgga cgctcaaaag
    30181 cctttgtaaa taaccgatca ttacctgttt cacaacttcg agagttgggg caatatctta
    30241 ttcatttaaa tgggcaacac gcaccacaat tactcctaaa aaacgaatat caacttgaaa
    30301 tagtcgatca ttatgccacg ttacagcctc tattgatgaa gatggcaaag caatatggga
    30361 attggaaaga actgaataag caggtaaaaa atttccaaaa acagtgccaa gaaaatgaag
    30421 ctcgcaaaca gctattagaa tatcaagttg aggaattaga tgaatttgca attaaagcaa
    30481 acgaatttga agaaatggaa gagaaataca atatcttgtc ttcttccgag gagcttactg
    30541 agctttcaga tgaaatcaca cgcttattaa gtgatgatga aatcaatgca aacagtctga
    30601 tttataaagc gttacgcaat gcagaaaact tggctgaggt tgatagccaa tatcagggca
    30661 ttttagatat gcttaatgag gccctgattc agattcaaga agcaagctcc gaagtggctc
    30721 aattagccag taaaattgaa caagatccgg aattactaca ggagctggat aatagaattg
    30781 cacaaactat atcgttagca cgcaaacacc atattcaacc gaatgaatta tggcaatatc
    30841 acgcgacatt aaaagcagaa ctacaggaat tactggattt tgaagataat gaagaacaac
    30901 tcattcaaaa tgaaaaacaa gcttaccaag agtgtataac attagcagaa gaaatttacc
    30961 aaaaacgtct agatgccaca cataagctct ctgaacaagt aacaaaacaa attaaacact
    31021 tagcaatgga aaacggtgag ttctttattc atatagaaca taatcttgaa aaaatgtctg
    31081 caaatggtgc tgactatgtc gagtttaatt tacgcagcaa tttaggacaa caaccccaac
    31141 cactggcaaa aattgcttct ggtggtgagc tatctcgcat ttctctggca gtacaagtgc
    31201 ttaccgcaaa taaactttct acgccaacta ttatttttga tgaagttgat gttgggatta
    31261 gtggctctgc tgccactaca gtcggaaagc tactgcgtaa gctaggcgaa aaatgccaag
    31321 tattatgtgt aacccattta cctcaagtgg caagttacgg caaccagcag tttaatgtac
    31381 aaaaatatgt agaaaataat caaactgaaa cgcaaatgac attactcaca gaagcagagc
    31441 gtattcacgc cttggctcga ctattaggcg gaagcgaaat caccgaaacg gttttagcta
    31501 atgcaaaaga aatgctaaat ttagcgaaaa ataacgaata gccaaaaaga aaaatcgcat
    31561 caatgaatat tgatgcgatt atgaatttgg tggaggtaaa cgggatcgaa ccgatgacct
    31621 cttgcatgcc atgcaagcgc tctcccaact gagctatacc cccttaatac aagcggaata
    31681 atatgctttc cgcttgaaga tgtcaatggc tttaatcaag caaggtgttg attgattaaa
    31741 aactagctat tcgcctgaat aaactcaatc gctttttgta agcgagctaa ggttctttct
    31801 ttaccgacta atttggcggt gatatccatt gacggcgatt gcccacttcc tgttaccgct
    31861 aaacggaacg gcataccgac tttgcccata ccaatttcca gctcggcagc ggtttggttc
    31921 attgcctcgt ggatattttc caccgtccag tcattgcaag cggtcaattt ttccaataat
    31981 tttgcaagtg gagcaacggc ttcggctttg aagtttttag cagcagcttt ttcgtcgtaa
    32041 gccttaaatt cttgatagaa atagcggctg ctttcggcaa gctcttttaa ggttttgcaa
    32101 cgttcgccca gtacagggat aatttcttcc aattttggac cgcttgtgaa atcaatgcct
    32161 tgagtttgca tatgccattc aagatgtttt gccacataac ttggggcaag actgcggata
    32221 tagtgttggt tcaaccattg taatttttca gtgttaaatg cacttgccga tttactgact
    32281 gaatgaatgt cgaacagctc gatcatttcg tcacgagaga aaatctcttg gtcgccgtgt
    32341 ccccagccta aacgcacaag gtagttaatt aaggcttccg gtagatagcc gtcatcacga
    32401 tattgcatta cgctcaccgc accgtggcgt ttggagagtt tttgcccgtc atcgccgttg
    32461 atcattgaaa cgtgggcgta ggttgggatt ggtgctccta atgctttcaa aatgttgatt
    32521 tggcgtggtg tattgttgat atggtcttca ccccgcacaa cgtgagtaat gcccatatcc
    32581 cagtcgtcca ccaccacaca gaaattgtag gtcggcgagc cgtcagtacg gcgaataatc
    32641 aagtcatcta attcgctatt ggcgatttca attcgcccac gcaccgcatc gtcaaatact
    32701 accgagcctt cggtcgggtt tttgaaacgc acaacatgtg gttggctcgg gtcgttttgc
    32761 ggattgtgta agcagtgctg atcgtatttc gctttctctt tatttgcttc ttgctctgca
    32821 cgtacttcgt ctaaacgttc tttcgagcag tagcaacggt atgccgtgcc ggcttctaac
    32881 atttggtcga tcacttggtt atagcgatcg aaacgcttgg tttgaaagta tgggccgtgt
    32941 tcccacgcca aatccagcca ttccataccc tctaaaatag cggcggttgc ctcaggggtt
    33001 gagcgttcaa gatcggtatc ttcaatacgt aacacaaatt caccatcatt atgcttagca
    33061 tataaccaag agtagagcgc ggtcctcgcc ccacctacgt gcaagtagcc agttgggcta
    33121 ggtgcaaaac gggtacggac ttttacattc ggatctaagg ggaaaagggt ttcaattttc
    33181 attttatatt gcctaattga tgattcttaa gaaaaacttg tctattttac tacgcttgag
    33241 ggtgaatcat agtaaaaaat taaatttgcg gggtaaaaaa tcagcaaacg gggtaaaaag
    33301 aacaaaaagg gtttgacgaa acagcaaaaa tctctataat tcacttccac aacagcgggc
    33361 gattagctca gttgggagag cacctccctt acaaggaggg ggtcactggt tcgagaccgg
    33421 tatcgcccac cacttcaact taagtgtttc gttgttgtat cacaatctaa ttttgaaaac
    33481 gggcgattag ctcagttggg agagcacctc ccttacaagg agggggtcac tggttcgaga
    33541 ccggtatcgc ccaccacttc aacttaagtg tttcgttttc aaattccctt tgattttaag
    33601 tctcaagtgg gcgattagct cagttgggag agcacctccc ttacaaggag ggggtcactg
    33661 gttcgagacc ggtatcgccc accacttaag actaaaatca taaacttcaa atggcgattt
    33721 ttcgtatctg aagggcgatt agctcagttg ggagagcacc tcccttacaa ggagggggtc
    33781 actggttcga gaccggtatc gcccaccatc tatttagtat caaagcctaa aacttatatc
    33841 ataatctaat aaccccttta aagtcataac taaaaaggga aaataaattc agatggcctg
    33901 ttgcatctgc ataaatgcct ttctacgtgc agaaattaat ctttttctat ctttaatttg
    33961 cttaatttga cgcaggcgta atttccttgt cagtagggat ttctttttct tcaattttct
    34021 cataagaaga attctcctgt tttatttgac catctggtaa attttcttgt tcttgcatct
    34081 tttgctcttc aactcgttga taattaataa tactaccata ataacgccgt atattttcaa
    34141 catactgtaa tgcttcatat cctcgagcat agccgtattt tagattagca taataacgtt
    34201 tttccgcaag caatggcaga ttctttttaa catcaagcca attatccgga tcaccaccaa
    34261 gttgttttgt taatcgacga agatccaaca agtgccccaa tcccatatta tacgctgcta
    34321 ggctatacca aattctatct gcttgaggaa tagtttccgg catttgtctc attagcatat
    34381 gtaaatattc agatcccgct ttgatacttt gtgcagggtt agtcctatca gaaattttca
    34441 ttctatctgc cgtatctttt gtcagcatca taattcccct cactccggtt ggagaagttg
    34501 cattaggatc ccaatgagac tcctgatagg cgatagccgc cagcatttgc cattctaatt
    34561 cacctttatg tgcccgaaaa aggggttcat atttaggtaa aatagtttta attgcattca
    34621 aatagctctg aatatcaaca taatcaaact tagctaaatg attaaaatat ttctcttcta
    34681 ttcttgatat cagcccagtt tccatagaat aagacataaa atccagcagc acagattgca
    34741 gttcactata agaactattt gctaaatacc ataacactgg gttttcatca gttaaatcaa
    34801 accctacggc taaattagga ttaatatgct gtgcagcaga aatatcaacc gacattgcaa
    34861 ttgtatgtga aatttcacct tgtgcgactt ttaacaatag ctcttcttgt gtaaatttat
    34921 ccgagatctg ccattttagt tccggattat ccaccttaag ctgatttaat attggaatta
    34981 ccgcagaacc ggctggaata atcacatttt cccgcaaatc ggaaagtcga tatggtctag
    35041 cacttccctt tttataaaca agctgccaag aagccgaata ataggaagga cctatctgaa
    35101 attcatcgtt taattcagaa tggtaaagca gcccagcggc agcaatatca acatcattat
    35161 ttcttaaggc ctcaaacaat ttctcgctac tatcataagt tttaatatca agattgacct
    35221 ttaaatattg agcaaaggct gttgctaaat catattcaat gccacttttt ccttcaggac
    35281 caacaaagta agaaagtgga tggttgatca ttccaacctt taacgttttg ctttgctgaa
    35341 tttgtgtata atgatttgct tctgaatgaa tgattttttg ccaaggaaaa atcatatccc
    35401 aagcccacag taacaatgct acggcaataa aaaggcgagc aattaatcct ttcaatttaa
    35461 aatcctcatt atttcaagct aagcattaag cctatctgaa attacaa
//
LOCUS       DANLUQ010000006        34347 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.006, whole genome shotgun sequence.
ACCESSION   DANLUQ010000006 DANLUQ010000000
VERSION     DANLUQ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 34347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34347
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.006"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            61..279
                     /locus_tag="PXB27_000242"
     CDS             61..279
                     /locus_tag="PXB27_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5345072.1"
                     /translation="MARNKRVRDMSNFEIRGELMKIGKSLSQLGIENGLAKTTVRNAL
                     DKPYPKGEQIIAKAIGKTPQEIWPSRYE"
     gene            289..2259
                     /locus_tag="PXB27_000243"
     CDS             289..2259
                     /locus_tag="PXB27_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HDL5345073.1"
                     /translation="MKEWFLAKEIAGLSGVPELPNSVSRLATKEGWQKRQIQGVRGVT
                     YEYHLTSLPIETQQQLRLNAALAVIPQAAELQPKRDDPALIARLNNATDKGRDKAKGK
                     AEACMQLQAFLDQGFSYTQAEAGAATAKNVSQGSLKNWYYKVKGHPVHLWQAILISES
                     GRSKKPQLKAKITEEAWDCFLADYLRPEKPDLRASYRRTQAIAKQYGWQMASLQTFQR
                     RVLAEVPYEVILLKREGANAVAKLVPALQRTVKDILAGEWINGDGYQHNVFVKWHTGE
                     IVRPKTWFWQDVRTRKILGYRTSISENTDSIRHALMDVIFNVGIPKTLTLDNTRAAAN
                     KAMTGGIENRYRFKHTELDPKGIMPILGIDVHFTSILYGEGHGQAKPIERAFGRGGIG
                     EKIDKRPELSGFYTGRNAQETPDNYNGGKDGVDYNTFLKAIAAGIEEYNSQTERHTEM
                     CRGELSFNQVWERDYHPSNVRQASPEQLRLLFLQAETVSIKRNGSFTLKAAGKLYGLT
                     NVYWAESLIGITDKKVVARFDPDDLHGNVYVYNLEGQFLAEAVCREAKGFGDTSASRE
                     QGRLYQKVVKSAKAQAEALELLEAHELASLAPQVDVPEPIEKKVKEVLVKEDFIVDFN
                     TVRKATVVEETEEISIFDEVLLKQEKQLRIVE"
     gene            complement(2439..2927)
                     /locus_tag="PXB27_000244"
     CDS             complement(2439..2927)
                     /locus_tag="PXB27_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345074.1"
                     /translation="MLFRSLTDDEIHQIALDSARATFSGFKLKRANAIVMKNCITLFK
                     LSTINFCLAEYHSSFHHLTSIDDLDGIMPTVKKVAQMLSLQESKALIWLLYLKWLFVM
                     RQRSLQVLLENSADLIQGEVIAQPLPTFVKAVLAFQLETYHGLPEWIPEFDSAEFWQS
                     LS"
     gene            2959..3900
                     /locus_tag="PXB27_000245"
     CDS             2959..3900
                     /locus_tag="PXB27_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL5345075.1"
                     /translation="MTLIDQIKQHIAESGSSQGKVAKEAGINAGALSAYLNENYKGNN
                     EELEAKLIAYLERIEAKKREFVEAPSFIETKTANQIFGSLRFAQNTGVLAIIHGASGV
                     GKTQAAREYRKRYANVWLVTASPSRSSLSEVLYEIALELGMNDAPRRKGTLARLIVRK
                     MKGTAGLLIIDEADHLPYEALEELRLLQEEVVNESDIGAGVGLVLIGNDKVYTRMKGG
                     INPAHEYGRLWSRSAKRTSIQKTKKEDTQAVAKAWGLSENQEALKVMQAITETGGGLR
                     ILTHTLRLAAMVAKDRMIPLTADLIQLARKDLLSTGD"
     gene            3903..4217
                     /locus_tag="PXB27_000246"
     CDS             3903..4217
                     /locus_tag="PXB27_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345076.1"
                     /translation="MRTPKPKHSFNRTNQAACRFLKQTQKAIIRLNSLGFTVLNIDFT
                     RIKPRIEVEIGNNKHIAQGLIYEGKAYRYSFGKSEDLGRWEGYYTMLEGIRVWWRQAA
                     IH"
     gene            4229..4465
                     /locus_tag="PXB27_000247"
     CDS             4229..4465
                     /locus_tag="PXB27_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345077.1"
                     /translation="MKTLALLTALLGLVGCDLVQSKQAKNYIGNNLTEICIDNVVYLI
                     YSGDKKGGITPKVNKDFYPYTCTNKEERQNGKTR"
     gene            4449..4742
                     /locus_tag="PXB27_000248"
     CDS             4449..4742
                     /locus_tag="PXB27_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345078.1"
                     /translation="MAKPDETPITKAECQSQLAELGVQYKKLPMAITRHICNATTNIH
                     GKVIKVSVVERVGYGVQITAQGNEKSCLVTYEAMLGMAEAMGLFDEVKEQNND"
     gene            4864..5082
                     /locus_tag="PXB27_000249"
     CDS             4864..5082
                     /locus_tag="PXB27_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HDL5345079.1"
                     /translation="MNDELAAISASYAQHNRYQHYAEKAAEAERKGNYKEAQDHWEVA
                     KLSAKKTANRDWAEQRAEFCKRMHNKPF"
     gene            5092..5274
                     /locus_tag="PXB27_000250"
     CDS             5092..5274
                     /locus_tag="PXB27_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345080.1"
                     /translation="MTETVKAQLNSQLNEAIIQLIQAQKYLNQDDAIRSGVYIGTVQD
                     LLPKVHLKLLTANRKH"
     gene            5291..5680
                     /locus_tag="PXB27_000251"
     CDS             5291..5680
                     /locus_tag="PXB27_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345081.1"
                     /translation="MAITEEQWKEIKQKLDSIIGRVKFRYKEHLLTVDVVQIKRSLKL
                     AVYVDGEIDGAWTKEGHEIRPYLEEVWYRKERPFFNAKEKKEYRGLMSKKKLNEKIVT
                     YSPMFPSPTALTRQYKKLDGLELIEVI"
     gene            5729..6016
                     /locus_tag="PXB27_000252"
     CDS             5729..6016
                     /locus_tag="PXB27_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345082.1"
                     /translation="MNKNEFFKKYHLEGSLAILDLDANDDQSQFCYQALLEYRELPLD
                     TFLAEYSDDEDMTESEFIELMLDELEWIIRRAKNAKRFVKKRQKTMREEIM"
     gene            6135..6680
                     /locus_tag="PXB27_000253"
     CDS             6135..6680
                     /locus_tag="PXB27_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1018 domain-containing protein"
                     /protein_id="HDL5345083.1"
                     /translation="MTDKAKLIQLIHIAKSQLAMDDLSYREMLKRLTNKTSSTKCTVV
                     ELHKVLHELQTKGAKVKYFAKRSKKPTAYSPATGETAVKSQIAHKIRAVWITMGKHGF
                     LNDPSEKALNAYMRKVINNKTRPTILLNVSALEAGDASYMLEILKKWHKRVMIKALSK
                     SCTVDPKISYDELVEWYNEVM"
     gene            6667..7200
                     /locus_tag="PXB27_000254"
     CDS             6667..7200
                     /locus_tag="PXB27_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345084.1"
                     /translation="MKLCRCPICHSDIHLEALVEDEAGRELLGKISQLTHGVAQPMVA
                     YLGLFKPQKSNLNNSRALKILNDVLALYPCSLLLAQALSETVVSIRKKRQQAMENGQK
                     IEPLPNHNYLKSVYETQKVHFAVVRTGKTERETMKAELEKTRNAIAYVQRFVDLGREE
                     VVKNSPEYQIWLAHKPK"
     gene            7365..7724
                     /locus_tag="PXB27_000255"
     CDS             7365..7724
                     /locus_tag="PXB27_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL5345085.1"
                     /translation="MELVDIFEEKAPEVLTDLAKHIEVALEEKCQFSPEKAKQIGIDI
                     AQQMAQNWGGEVIYIPRNLLMALNERDMKIYEEFNGNNHRELARKYNVSMQWVYKIVK
                     KVHKDEVARRQMSMFEM"
     gene            7860..8369
                     /locus_tag="PXB27_000256"
     CDS             7860..8369
                     /locus_tag="PXB27_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HDL5345086.1"
                     /translation="MSQNLTFRQIFDRLIGHEGGYVNHPQDPGGETHWGITKRTAVAN
                     GYMYAMKNMTREQAYQIYEKAFWQRYRCAELKPAVAYQFFDAVVNHGFGNASRMLQRA
                     VGVADDGIIGNITLQAINAKSESDLLMLFNAERLKFYTKLRNFPTFGKGWVNRVAGNL
                     EYAAQDNEV"
     gene            8372..8641
                     /locus_tag="PXB27_000257"
     CDS             8372..8641
                     /locus_tag="PXB27_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2644 domain-containing protein"
                     /protein_id="HDL5345087.1"
                     /translation="MAKLFKELLSTNGTLSTTSTVQFFGTLCVFGLMVYAVITQQPYA
                     ESLLNNVLIYLFGATTAKGVVTSYQAKIKGGIHKQQSTFKEEEGC"
     gene            8635..8982
                     /locus_tag="PXB27_000258"
     CDS             8635..8982
                     /locus_tag="PXB27_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2681 domain-containing protein"
                     /protein_id="HDL5345088.1"
                     /translation="MLMQQILGVIGVIAAILSYALFKSWQLKRERKKNAILQAEKQQQ
                     AVEIEQKKAEVKYVYISKKNNEKVTRSSASDIDDKLQQHGYFRDNNRLHGVQSDLSEP
                     CGYAGNETSDSCS"
     gene            9009..9161
                     /locus_tag="PXB27_000259"
     CDS             9009..9161
                     /locus_tag="PXB27_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345089.1"
                     /translation="MKYIKKLRKRWQIWRFLKNHPEVAQRRDLLAIAIKQGVENPVGR
                     ARRREP"
     gene            9161..9496
                     /locus_tag="PXB27_000260"
     CDS             9161..9496
                     /locus_tag="PXB27_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2730 domain-containing protein"
                     /protein_id="HDL5345090.1"
                     /translation="MDLFTVFWESIRANFGFISALLTIVVTAFWLKLDSKYAKKNDLG
                     ELLEIAKSHDNRLTTLETKVENLPSAVDVEKLKTLVTDVKGDTKATSKQVDSISHQLG
                     LLLEAKLKE"
     gene            9498..9794
                     /locus_tag="PXB27_000261"
     CDS             9498..9794
                     /locus_tag="PXB27_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345091.1"
                     /translation="MSLRDLLTQDQRLVILRSLAEAGYDANESIISDCLDLYGHDISR
                     DLVRTHLSWLEEQGLLTLERLRDGYMVASITQRGLDVAQGRVVVSGVKRPRPKI"
     gene            9817..10389
                     /locus_tag="PXB27_000262"
     CDS             9817..10389
                     /locus_tag="PXB27_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3486 family protein"
                     /protein_id="HDL5345092.1"
                     /translation="MSEKNTRGRASKVDLLPPNIKTQLAMMLRDKQYSQSQILEEIND
                     LIRDCGLPDEMLLSKTGLNRYASRMERLGAKIRNAREVAEIWTKQFGEMPQTDIGKTL
                     MEMVKQIAFETSLKLGEQEGGIEPKQLALLSSAIQRLEQAESLSFKREQAIRKEVAQQ
                     AAETAEKVVVQAGLSAETVRTIKEQILGIA"
     gene            10389..11945
                     /locus_tag="PXB27_000263"
     CDS             10389..11945
                     /locus_tag="PXB27_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HDL5345093.1"
                     /translation="MALLNNRPLNELAPECRSFLESIHAFNPNELLLGYQKRWIADDS
                     QLKIAEKTRRCGLTWAEAADNALIASTRKSDGGSDVFYIGSNKEMAREYIDAVAMWAK
                     AFNRVAGEIQEEIFEDEDKDILTYVIYFSSGFKVKALSSNPKNLRGMQGVVVIDEAAF
                     HEYLAEVLKAALALTMWGAKVRIISTHNGSENLFNELITDSRAGRKRYSVHTITIEDA
                     CRDGLYQRICQVVTKKTWSPEAEKEWIENLLNDTASEEDALEEYFCVPKFGSGLWLSR
                     ALIERQMSEATPVIRFTAKDGFSLVAEPTRYAEIAEWCELELEPILATLNPDLLHFMG
                     EDFARSGDMTSLVVLAQQQNLVRKVAFIVELGNMPYKQQEQIVMFMLKRLPHFAGGAF
                     DARGNGGYLAEAARDEFSTLIDCVQLSEKWYREHTAPFKAALEDGELEAIPKDADILA
                     DLRSFQVVKGVPRIPDKRTKSADGKSKRHGDTAISLLLAHYASRQLVQMPVEPVSRRP
                     RHSQQLTEGY"
     gene            12064..13518
                     /locus_tag="PXB27_000264"
     CDS             12064..13518
                     /locus_tag="PXB27_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 family protein"
                     /protein_id="HDL5345094.1"
                     /translation="MAKKNNLISEIATRARSIDYWAFGYYLPNPDPILKRMGKDISVY
                     RELLSDGQVRSGVRRRKAAIKGLEWRITTTNNEKVDEQIYQIFNRLPMNNLITEMLNA
                     ALYGYHISEVIWAKEGELFVPTEIIGKKPEWFMFDDENLLRFRTKENWADGELLPDDK
                     FLLTTQEATQDNPYGLGDLSLCFWAATFKKGGFKYWLEFTEKYGSPWLIGKHPRQAHN
                     TDKERLADALEGMISSAIAVIPEDSSVEIKDAAGKSASTDAFEKFLNFCKAEINIALL
                     GQNQTTEQESNRASAQVGLEVMEDIRSDDQAMIEATFNTLLQWIVKYNFNVEQLPKFE
                     FYEQEAINTDQVERDSKLHAMGVRFSKAYFEREYGFEAGDIEVQAVEKAEKSANTTEF
                     SEGQRRTQVPMPVPILEQLEHEGEAMVDDWLQGVRDKLAQADSLEDFQSQLDSLIPEL
                     SFAEYGELLALGSTVAELAGRQSVNDEIKAKRNA"
     gene            13511..14788
                     /locus_tag="PXB27_000265"
     CDS             13511..14788
                     /locus_tag="PXB27_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HDL5345095.1"
                     /translation="MRKFTFKQQAQYFEKKLNLRTNSYLDILGEEHDYFFVVAGANRN
                     EVLSEFRKAVDEAIHNGETLENFRKRFDEVVAKTGWEYNGGRNWRTRIIYDTNVYGAY
                     NRGRLQQHLELAEDMPYWEYQHNDNAHPRPQHVAWDGLVLRYDDPFWKYHYPIKAYGC
                     HCTVQALDDFDLKHDGKTVSPPPEIEFEQKTVGVRTGNSRVVTVPKGVDVGFTPHNFD
                     NLKAGRTANVDKLLMQKMVTADPIFASKAVSDLLAKEPVAKLHNQAFKNWVNDVANAK
                     GSALSQVENMQLLGVLPVAVIERLQQINLAPQSAIIAITKGDMVHALRELKQNTGIAV
                     PLEFWEKLPAKLQYPNAILLEKQQAETTLLFIYDVAQTKIAVKVNYDATLQDRHGAKG
                     RQKVLLNRAVTGSLFQDTTGLKSSKYEVLLGEM"
     gene            complement(14990..15334)
                     /locus_tag="PXB27_000266"
     CDS             complement(14990..15334)
                     /locus_tag="PXB27_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345096.1"
                     /translation="MMVNAEQAIELVYDLLLSRQWLVTKAEKLPLDPLSEKEAVMFLY
                     TLDQQTEASWLQLTPEQRATANGLIMDFIAKCLTSTKQWLVSDNIVPELQAIEIIKHE
                     IFLSHNSLVMPN"
     gene            15398..15862
                     /locus_tag="PXB27_000267"
     CDS             15398..15862
                     /locus_tag="PXB27_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HDL5345097.1"
                     /translation="MIKITLNNTQAVKSLQQIANQLEQPRRLYGILGETLKKIHTARF
                     KAEQDPDGNNWKPLASSTLALKRKRGKSTKILRQDGYLADKTAYNVTSDNVEFGSQEV
                     YARLHQFGGKAGRGKKVTVPARPWLGVGDKDEQLLLRKAEWHLGQIIGRMGK"
     gene            16097..17212
                     /locus_tag="PXB27_000268"
     CDS             16097..17212
                     /locus_tag="PXB27_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDL5345098.1"
                     /translation="MTQIEIFKAGKRYDANGTLIDITPEMLQQTVAAYNPEFHEAPLV
                     IGHPKSNNPAWGWVKALSLEGDVLKAEVDQLDAEFSEMVASGKFKKVSAAFYLPDSPN
                     NPHKGVLSLRHVGFLGAMPPAVKGLKQVEFAEDDDFVVFSEWGVASLFSRFRDWLVSE
                     FGLEKADKALPPHEIDWLKEEAMRSQISKQVHAEQATPEPIFNEPAQPEGEPEMSAEE
                     KEELERLKAENEQLKAEKAQAEAEKMEAELEAEKAGNAEFCEGLVKQGKLAPVAKAAF
                     VQALNNLSVLKAGREPEFNEGEDVVSQFKSAMSQSPQIIQFGEYATADKANPQEAEAV
                     EYAEGTDPVSIEADQKVRAYMKAHNVGYAAAFNAIYR"
     gene            17243..18169
                     /locus_tag="PXB27_000269"
     CDS             17243..18169
                     /locus_tag="PXB27_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007524268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HDL5345099.1"
                     /translation="MTTHNLQKLRVQDPVLTELAQGYHNNELVAETLMPTVEIEKEAG
                     KIPTFGRLAFRLPSTVRNLRGSSNRLDPEDIGAIDVALEEHDVEYAIDYREENEAIFS
                     LRQFALNTTQDVIALGREKAVATLALDESRYETDNKVTLSGTSQFSHKDADVFKVFDT
                     AIRAVKRAIGRKPNVCVISGDVWAALKEHPAVVEKLKYSQVAIVTPEVFAKLIGIETV
                     KIGEAVYEESGQLKDIWSKDIVVAYVAPRSTGGKGTVYEPSYGYTVRRQKGLFVDTYK
                     ESGGKIEVVRTTDIHKPHLVGAAAGYLIKAAV"
     gene            18238..18519
                     /locus_tag="PXB27_000270"
     CDS             18238..18519
                     /locus_tag="PXB27_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345100.1"
                     /translation="MAKFIVLHTPILHDGKRYEVGAEIELTAEQAENNAINLKAVEAV
                     PKPPKNASKPEDEPKPEGEPKPEDAVTEDDVKLAEAKIAEAEQPKGNKK"
     gene            18519..18953
                     /locus_tag="PXB27_000271"
     CDS             18519..18953
                     /locus_tag="PXB27_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 domain-containing protein"
                     /protein_id="HDL5345101.1"
                     /translation="MYVQIDELLTAFSRKIVVQLSNDEPTATEPDLKMLETAVKVANE
                     RIDAALRSRYTLPLADVPTLINQHALTLARYWLYARRPETKMPDTVKETYQQAVKELE
                     QIATGKLHLGIAESSQILEDDVLADNSEYEVRANARIDTSRY"
     gene            18959..19456
                     /locus_tag="PXB27_000272"
     CDS             18959..19456
                     /locus_tag="PXB27_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp37 family protein"
                     /protein_id="HDL5345102.1"
                     /translation="MSATQPILEDICQHLRQALPEWEVELFPNDPSTYHLSHINGAVL
                     ISYLASKFEKPRTTQAVLQVRHVQIALTVLTRDLHNDVGALDLLDKLRLLVVGFKPQN
                     CGECWLIDEFFNGSSEETGLWQYQLILQTETQQVQHQAVENLPKFVTAHLRRQDQPGS
                     PGLKT"
     gene            19541..20926
                     /locus_tag="PXB27_000273"
     CDS             19541..20926
                     /locus_tag="PXB27_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HDL5345103.1"
                     /translation="MTFHHGSETERVNGGSVPVSVVDSAIIGIVGTAPIGAVNELTVC
                     LTKKDFAKFGTILNQGFTLPDAFDILARYAAGKVYVVNVLDPSRHRTTVTDEVLTLDR
                     NTLTAQTAKTGIIAVTRVKDGANVLNADRYSVDLQTGVVTFVSAPSAPKISYIYADPS
                     KVTEEDIKGGVDSLTGKRKGFELLRDGFNLYGADAKILICPEFDKTASCAAALATLAD
                     QLHAKAYIQLPKGTSLSKAIQGRGPAGTINASASNENVRHFFPYALGSSNTLESLATH
                     AAGLRMLIDVEKGYWFSTSNKELKGVIGLEIPLTARVDDKQSETNLLNAVGITTIFNS
                     FGTGFRLWGNRSSNFPTVTHISNFEVASRTGDIIDESIRQAELQFIDLPIDDALLDSF
                     LETIDTFLRTQKSLVGYSVSLDYEYDLVDAFSRGQVPIVYEYTPKIPGERISNKSVMT
                     RKYLANLVSQR"
     gene            20937..21452
                     /locus_tag="PXB27_000274"
     CDS             20937..21452
                     /locus_tag="PXB27_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HDL5345104.1"
                     /translation="MSIAINQIVNGNVYINGNSQMGRVNEVKIPDIEFEFIEHKGLGM
                     HGVIKLPAGTNAIEAEITWDSFYPEVRAVMLNPYKYTQLQIRSNLQVFNSQGLAAEEP
                     MVTIMNVSAGKIGGTGHKNKENAEFADTLNVHSIKQTVAGRELLFIDVLANIYRVNGV
                     DVLQKFRTNIG"
     gene            21548..21862
                     /locus_tag="PXB27_000275"
     CDS             21548..21862
                     /locus_tag="PXB27_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HDL5345105.1"
                     /translation="MANAAQQVLTNLRVQTTYKLQYPIALPDGRVIETLDVRRPKGKD
                     FRLLDEKGFDPEKDGVKILRFYIQQLTTLVPEDIDELDAADIKGLNNLLEDMLIEGKS
                     DK"
     gene            21859..22011
                     /locus_tag="PXB27_000276"
     CDS             21859..22011
                     /locus_tag="PXB27_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HDL5345106.1"
                     /translation="MSELDTVYADLAWWFKFSPSELLELDLVDIPKWVEQMNRQIKAG
                     YGQILR"
     gene            complement(21990..22292)
                     /locus_tag="PXB27_000277"
     CDS             complement(21990..22292)
                     /locus_tag="PXB27_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345107.1"
                     /translation="MLTKLNNRITEWAGFEACVTRTECTAKWLLAVALVGWSIFYWLD
                     AIQSIQSLSEGVSFVLIYGLILTILAWVGVYLAWGVAILLTLATEPFIWLYRKICP"
     gene            22340..24997
                     /locus_tag="PXB27_000278"
     CDS             22340..24997
                     /locus_tag="PXB27_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL5345108.1"
                     /translation="MANNLAIGMVIGASLSSGFHGAFSGAKKKLEQLEQAVAKNQQSH
                     SKLGSELTALRSKQATLYAEMSRASSKGGAGLALMQQEYKKIGKEIANISREQRKWST
                     ELEKSIAKQTKLRNAMERHTQAKENRDELKGKIIGTVASAAMGVGVMKTYMEQEEAAN
                     NLKISMLKADGSFGKFEEIGKIADTLGTDLPGTKKDFYKLAMALKKQGVSDDVLTGGA
                     LKTSAELNVLLDMDQESGGEFLAKFMESHRLNENELPQAADYLQRAMFAGGLSKEQMY
                     ESMKYYAPKLNSMKLTGAENTEKVLAIEAMAGQQGLEGSTFGTGLNMMLSRMNKGPEM
                     LKAAAKGMKAEAREMMEAVGVEFNFWDKNGSFKGIDGMLAEMTKFEKIRKKFGDKGVG
                     LVAEELFGIEGGRLADILAMKGQKGLDEMLSKMREQASLQDRINLKTNTLGAALESLG
                     GAWETFVGDIGSVFAEDIKGFSKGLQSAVEWLVPFVSKHKEAIKFVGGLVAGLVILRG
                     VLQAGSFVIQTVTMAMNALKIAAMTNPIVLVIGAIAAGAYLIYSHWDSIAPWFANLWQ
                     NVTAYFNQFWTWVKGVWSGVTDWVSSVWNGVATFFGQLWTTITSLFSVAWTNIQTIWG
                     GATAWFSGLWDGIKSAAFGMFDSISQKVSSVVASIKSFLGFGDDVDKMGAKIGNVATQ
                     VISKEHSGQLSKTADMAANTGFSSGGFTGQGGKYTPAGIVHKGEYVMTKEATSRLGVG
                     MLDSLNYGGAMGFTQPVLQPILGKQKGFFGSLWDDVKFGANFVGNLLGLNQGSLKTPD
                     FNPNATNASGQNSSIFSNYEPLNRNAVIQQESSQAPQIVVNFNPTINVNGNATQEAQS
                     QIMQQLQMSAYEFEKLLNRVLDQRSRRAY"
     gene            25007..25930
                     /locus_tag="PXB27_000279"
     CDS             25007..25930
                     /locus_tag="PXB27_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5345109.1"
                     /translation="MYFMLGNIAFEPVNLTEFSEQHTAEFAEHAVLKGKPRLQAMGEK
                     LNELNFSLRLHHTIGGVESRYQALLEAKAKQAALALIWGSRYKGDYVIVDISSTTLFT
                     DGRGNALAREMQISLKEFVGDNDGGVLGEALNFGGGSLLGSILPSGAVTTLSQIKTAV
                     SRGVELYNSGKRLVDEVQNTVAIIRQLKNDPATALAYLPAALANLDGALGHFGNLTGS
                     SATLGGLQEKLPAAVVFSEEIGEIYQSLQTMRSSLGNASANSWDNWFTPAESALTEVT
                     DSFDHLAKPVAKMTAWIVLRTDEEAENDTVA"
     gene            25914..26141
                     /locus_tag="PXB27_000280"
     CDS             25914..26141
                     /locus_tag="PXB27_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HDL5345110.1"
                     /translation="MTQLLKHIVKQGERWDNLSYQYYGNAFEYGRIIDANPHIGFCEV
                     LPIGVTIYIPVLNVKPTNNENMPPWLRGRDA"
     gene            26134..27198
                     /locus_tag="PXB27_000281"
     CDS             26134..27198
                     /locus_tag="PXB27_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345111.1"
                     /translation="MRNVPKPDFSLFYEKTNITAEIEPHLVQLTYIDHLEGQSDELTV
                     EFEDIQGKWIRQWFPTQGDKLKAAIGYQGEFLTDIGEFEIDEVEYSYKPSTINLKALS
                     TGISKANRTLKPKAYENTTLAQVVAKVADNLKLKVVGKIKAIPIKRITQYQERDVEFL
                     ARLAREYHHSFKIVGNQLVFTDKSELGQSEPVLMLEERDTISLRLRDRIKDTAKAVEI
                     SGYDTSGKKVVKKRKKAKALRPNIKQAKSASEDTLKIITRGESQEQIDARGEAALATQ
                     NEDQAAGDIELIGNPKLVAGATILLRNFGVFSGKYLIKSSRHTISRSGGYTTHIEVRM
                     LEFIADDLITLGMEAGNANA"
     gene            27185..27721
                     /locus_tag="PXB27_000282"
     CDS             27185..27721
                     /locus_tag="PXB27_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HDL5345112.1"
                     /translation="MQTHNFGATYQEGIVSAVDPKTHKVRCKIPALEDLETAWLSFLT
                     PNAGGNQFYCLPDVGELVAMLLDAQGEGGCVFGAIYNEQDPTPVADSDMWVKKFSNGT
                     EISHNRKTGDVVVKTNGHLTATAQGGATINANTVVNGTLHATGKITSDEEVSAPVVKQ
                     GTVELGTHKHGGSPEPNK"
     gene            27776..28138
                     /locus_tag="PXB27_000283"
     CDS             27776..28138
                     /locus_tag="PXB27_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HDL5345113.1"
                     /translation="MNRQTSIQLTHWQLAPSTDTVEVVQGIDDIHLCIVNILSTQKGT
                     DILRPEFGSDHFRYIDYPEDVAIPNFVREITTALQKWEKRIEVEEVLVDGSAPHFIFT
                     VMWHLTDDVYREIYRTTV"
     gene            28148..29251
                     /locus_tag="PXB27_000284"
     CDS             28148..29251
                     /locus_tag="PXB27_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDL5345114.1"
                     /translation="MTEAIKIIDDDVKTVLAEAIADYEKRTGKILQPAHIERSIIQTY
                     AYREQLLRKGINHAFLQTFPQFATGIALDLCGETFGCYRLRDKPARTLLRFSVSGEHT
                     AIVIPKGTQVAINDELYFSTLNDDVITPLITYVEIDAECNQVGVIGNGWESGRVSKLK
                     TALATNYKVTVENIDITSGGLMQEEDEPYRKRILAAPEAFSNCGSIAAYDYHVRSVSQ
                     DIADVNISTPRGGLVRIAVLTQKGLPDTRLLDDIKKHVSAERLRPLCDMVEVIKPTER
                     RYQITARLILLDGYREDLVKAKAREALQLYLSDKTKKLGLDIVPSAIISALRVEGVYD
                     VNLIQPVKTIINETEWANCTAISVEVEEERRDG"
     gene            29244..29810
                     /locus_tag="PXB27_000285"
     CDS             29244..29810
                     /locus_tag="PXB27_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5345115.1"
                     /translation="MASLHYADIIVRDPKYKALADLSKRLNLLDTSQIMTTLVDLLGD
                     ECIPLLAEKWSVTGYDGLLVADSENSKRALIKRAIELHQLKGTPWSIREVLRRLGFGE
                     VEIDEGLKNRLYESTYVSGIPENERWAHYAIRLSNPITNEQARNIRKVLRNFAPARCS
                     LAVLDYKADPIRYNNKATYNGTYNHGSS"
     gene            29820..32099
                     /locus_tag="PXB27_000286"
     CDS             29820..32099
                     /locus_tag="PXB27_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HDL5345116.1"
                     /translation="MAGVQETAQWENHVYRIEENDPVHGGENGVTNKPIKHLANRTLY
                     LRRLLTEAGQRINPKKITATSKNSNDLTGHTHEIDHASLTQKGIVQLNASIQSTSTTE
                     SATPSAVKAAYDKAATAEQKATLANEKVEAIKVGGRNLLIQSNTPHNGNAYSVRYALA
                     EAPKVGEEIVVTLWGELGSQRTGIGVYNSQGATELAKLVKIRDGVYQGKGIWRKPMSE
                     GRERTPNDTHLNVYFYPKTATDNNRIERIQLERGSIGTDWTPNEADLKAEISAIRTKL
                     IPVRGGNVATTLNAAKRSDWFREMQIPYQSGWGYYTQAFHNSGTQHNYSNLPLTGRYY
                     FIAELYHNSGYSHLRITYPSLKRTFESAINLNNEDLILDWREVVFLKDGLYQGTFKTT
                     GTLESAYRLRAARDDERYQPYLQMVDMGYDLANPPVGKNKIIGSVDYYVKNGTSESSK
                     ATMMAAAMPDKNVFWEVALWNAAQQRNVLFKGYSKTNNLSIGKTTDNERDRVQVNGTI
                     QATAPADNANNDQVPTTAWVRKMAKILADGKVSKAGDTMTGHLFIKNGEYSAVHTYNT
                     SGWFAKWEAAPLSDSHFAAIVYANDRETVINRVLIPKKNGTVALINDIADTVRAAVSR
                     SDKSRSRTFPLQTGKKYTTTGSVTIYPDGRIVQIFHLKNIKAAWFDYEASAVGDRHRV
                     VNIPLWTAMPNKIFDVDVKTVRPSNNASTYYIEAAEWLSAWQIYGNDTNKSSVNINLS
                     RFRGGKDEDMDLYVTVEGY"
     gene            32100..32702
                     /locus_tag="PXB27_000287"
     CDS             32100..32702
                     /locus_tag="PXB27_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345117.1"
                     /translation="MAYFVNVIDEQGNYELIDDEFIGLYPDLDFNTLPKLTDKQYQTY
                     LAKANGKERFVNGEFVYEQIKVDMQAVISAEKSAKLAEINQKAQAFINDLAKYNETPK
                     FERDTWLEQAKEAKAWVADPTVQTPTLELIAQMRGIPLDTLRQKAYEKAMAYQTVAAI
                     VAGQRQGYEDRLEQAETLEQIQAIEPMYQLPQGANNEQTD"
     gene            32686..32961
                     /locus_tag="PXB27_000288"
     CDS             32686..32961
                     /locus_tag="PXB27_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345118.1"
                     /translation="MSKLTKLKSWSYHVLIAIDQLFNALTGGAADETFSSRCYRGAVL
                     AENPKKRWRFWHSFLNCLFLDPKHCYAAYKAEVHRKQYPKAFRLGGI"
     gene            32961..33248
                     /locus_tag="PXB27_000289"
     CDS             32961..33248
                     /locus_tag="PXB27_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345119.1"
                     /translation="MKKQNLKLVRGDDVSFRFSIKDSTGQPLALKDLWFDLHLVSYQG
                     EVLIALSTDDGSIETAEAGQMVLHFSHQQTARAEWTSANYDLQLTHDYTRV"
     gene            33258..33422
                     /locus_tag="PXB27_000290"
     CDS             33258..33422
                     /locus_tag="PXB27_000290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345120.1"
                     /translation="MTSIIELDLTDDLIIEVQLDSTIITGDSFLANLPDLLQIYNQAK
                     SDSSKELNND"
     gene            33415..34119
                     /locus_tag="PXB27_000291"
     CDS             33415..34119
                     /locus_tag="PXB27_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345121.1"
                     /translation="MTKHKNHENHPIAITFYQLGKDVGDINRRLAVIEQREQSAQPSQ
                     PQAEAVTMPQQDAVAINNINAVLEAVEKYKTFEKYNTQWQERFEKFLSRNPNDYELSE
                     EQRQKLYHTMCQHLWQVNFTEINSKQYELPIPQQFQGKFGYIVKTSGGSTEISFELLS
                     ETHSTYLYPDSYIPEGGGDVMLKPAYIYLVIPDLVAMREPEVVGVSPLKLNILQKHLL
                     TPQKLQEQKPLNTKAN"
BASE COUNT        10835 a         6860 c         8109 g         8543 t
ORIGIN      
        1 agttaaatga tttacatcat ttaagtagtt tctaaaacta actttcaagg atctcacaaa
       61 atggcaagaa ataaaagagt tcgagatatg agtaatttcg aaattcgtgg tgagctgatg
      121 aaaatcggta agtccttatc tcagttaggt attgagaatg gattagcaaa aaccacagtt
      181 cgtaatgcgt tagataagcc atatcccaaa ggggagcaaa ttattgcaaa agcgattggt
      241 aaaacaccgc aggaaatttg gccctctcgt tacgaataag gagcttttat gaaggaatgg
      301 tttttagcca aagaaatcgc aggattatct ggagttccag aattaccgaa tagcgtttca
      361 agattggcaa ccaaagaagg atggcaaaaa cgacaaattc aaggtgtaag aggtgttact
      421 tacgaatacc acctcaccag cctacccatt gaaacccaac aacaacttag actaaacgcc
      481 gcccttgcgg taataccgca agcggcagaa ctacaaccca aacgagacga ccccgcactt
      541 atcgcaaggc tcaataatgc caccgataaa ggcagggata aagcaaaagg caaggcagaa
      601 gcctgtatgc agttgcaagc ctttctcgac caagggttta gttatacgca ggcagaagcg
      661 ggagcggcaa ccgctaaaaa tgtgtcgcaa ggttctttaa aaaattggta ctacaaagtg
      721 aaaggtcacc ccgtccattt atggcaggcg atcttaattt ccgaaagcgg taggagtaaa
      781 aagccgcagc tgaaagccaa gattactgaa gaagcgtggg actgcttttt ggcggattac
      841 ctccgccctg aaaagccgga tttacgggca agttaccgcc gaacccaagc catcgccaag
      901 caatacggct ggcaaatggc aagtttacag actttccaac gccgagtgtt ggcggaagtg
      961 ccttacgagg tgattttgct caaacgggaa ggagcaaatg cggttgccaa gttagtgccg
     1021 gcactacaac gcacggtgaa agatattctc gcaggagaat ggattaacgg cgatggctat
     1081 caacacaatg tgtttgtgaa atggcacacc ggcgagattg tccgccctaa aacgtggttt
     1141 tggcaagatg ttcgcacccg caagatttta ggttatcgaa catcgatttc ggagaatact
     1201 gacagcatac gccacgcatt aatggatgtg atttttaacg taggcatacc gaaaacgctc
     1261 acgctggata acacccgagc agcagcgaat aaggcaatga ccggtggtat tgaaaaccgc
     1321 taccgcttta aacataccga gttagacccg aaaggcatta tgccgatttt agggattgat
     1381 gtgcatttta cctcaattct ttatggtgaa ggacacgggc aagccaagcc gattgaacgt
     1441 gctttcgggc gaggcggaat tggcgagaaa atagataaac gccctgaatt aagtggtttc
     1501 tatacgggca gaaatgcaca agaaacccct gataactata acggcggtaa agacggtgtg
     1561 gattacaaca cattcttgaa agccatagca gcagggatag aagagtacaa ctctcaaacc
     1621 gaacgccata ccgaaatgtg ccgaggagaa ttgagtttta accaagtttg ggagcgggat
     1681 taccacccaa gcaacgtaag acaagccagc cctgaacagt tgcggttact gttcttacag
     1741 gcagaaacgg tcagcattaa acgcaacggc agttttacgc tgaaagcagc cggcaaactt
     1801 tacggcttaa ctaacgttta ctgggcagaa agcctgatag ggataacgga taagaaagta
     1861 gtggcacggt tcgaccccga tgacttacac ggcaacgtgt atgtgtacaa cctagagggg
     1921 caattcttag ccgaagcggt ttgccgagag gcgaaaggat ttggtgatac cagtgcaagc
     1981 cgtgagcaag gtcgcttata tcagaaagta gtgaaaagtg ccaaagctca agccgaagcc
     2041 cttgaattac tggaagctca cgaactggca agcctcgccc cacaagtgga cgtgcctgag
     2101 ccgatagaga agaaggtaaa agaggtgctg gtgaaagaag attttatcgt ggatttcaac
     2161 actgtgcgta aagcaacggt ggtggaagaa accgaagaga tcagtatttt tgatgaagtg
     2221 ttactgaaac aagagaaaca acttcgcata gtggaataac tagcggtcag tttttgcaaa
     2281 aaatttgcaa atttttctaa aaaaacgacc gcttgaaatg agatttaaac agggtttaaa
     2341 cgcctttaag gagtaaaaaa tgacattaac agggcaagct taccgagtga ttttcaagga
     2401 actcaaagag caacaagaga gtgaatttga acaggctttc aagaaaggga ttgccaaaat
     2461 tcagcagaat caaactcagg aatccattct ggtaaaccgt gatatgtttc aagctgaaaa
     2521 gctaacactg ctttcacaaa agttggaagc ggttgagcaa ttacttcgcc ttgaattaaa
     2581 tcagccgaat tttcaagcaa aacttgaaga ctacgctgac gcattacgaa caaccatttt
     2641 aagtaaagta gccaaataag agctttagac tcttgaaggc ttaacatctg agccactttt
     2701 tttactgtcg gcattatacc gtcaagatca tcaatagaag ttaaatgatg aaaagaggaa
     2761 tggtattcag ctaaacaaaa attgattgta ctaagtttaa acaaagtaat gcaatttttc
     2821 atcacgatgg catttgctcg ttttaactta aaaccagaga aggttgcacg agcagaatca
     2881 agggctattt gatgaatttc atcatctgtt aaagaacgaa agagcataaa aacctccaaa
     2941 caaaaacagg acaccattat gacattaatc gaccaaatta aacaacacat cgccgaaagc
     3001 ggcagttctc aggggaaagt tgccaaagaa gccgggatta atgccggggc gttatcggct
     3061 tacctgaatg aaaattacaa aggcaataat gaagaacttg aagcaaagct gattgcttat
     3121 ttagagcgta ttgaggcgaa gaaaagagag tttgtcgaag ctccgagttt tattgaaacc
     3181 aaaaccgcaa accaaatttt cggttcgtta cgatttgctc aaaatactgg cgtgcttgcc
     3241 attattcacg gtgcgagtgg cgtgggtaaa acacaagcgg ctagagagta tcgtaaacgc
     3301 tatgcgaatg tatggttggt taccgcaagc ccttctcgtt cctcattaag tgaggtgtta
     3361 tatgaaattg ctcttgaact tggaatgaac gatgcaccac gccgcaaagg gacattggct
     3421 cgtttaattg ttcgcaaaat gaaaggcaca gcaggtttat taattattga tgaagcagac
     3481 catttacctt acgaagcctt agaagaatta aggctgttac aagaagaagt agtgaatgaa
     3541 agtgacatcg gagctggtgt tggcttagtg ctgattggca atgacaaagt ctacacccga
     3601 atgaaaggcg gaattaaccc tgctcacgaa tatgggcgac tttggtcacg cagtgccaaa
     3661 cgcaccagta ttcagaaaac caaaaaagaa gatacccaag cggtagccaa agcgtggggg
     3721 ttgagtgaaa accaagaagc actcaaggta atgcaagcca ttactgaaac tggtggcgga
     3781 ttacggattc tgacacacac cttacgatta gcagcgatgg tagcaaaaga ccgaatgatt
     3841 cccttaactg ccgatttaat tcagctcgca cgtaaagatt tactctcaac aggagattaa
     3901 caatgagaac cccaaaaccc aaacacagtt ttaaccgcac caaccaagca gcgtgccgtt
     3961 ttttaaaaca aacccaaaag gcaattatcc gcttaaacag tcttggcttt acggtgctga
     4021 atattgattt tacccgtatt aagccccgca ttgaggtgga aatcggcaat aacaagcaca
     4081 ttgctcaagg cttgatttat gagggtaaag cctaccgcta cagcttcggt aaaagcgaag
     4141 atttaggcag atgggaaggc tactacacca tgctggaagg tatcagagtg tggtggcgac
     4201 aagcagcaat tcactaggag aaaaacagat gaaaacctta gcactattaa ccgccctttt
     4261 agggcttgta gggtgtgatt tagtccaaag caaacaagct aaaaattaca tcggcaacaa
     4321 tttaactgaa atctgtatag ataacgtggt gtacctgatt tattccggtg ataaaaaagg
     4381 cggaattacc ccgaaagtaa ataaagattt ttacccttat acctgtacaa acaaagagga
     4441 aagacaaaat ggcaaaaccc gatgaaacac ccattacaaa agcagaatgt caatctcagc
     4501 ttgcagagtt gggggtgcaa tataaaaaac tgccaatggc aattacaaga catatttgta
     4561 atgccacaac caatatacac ggcaaagtta taaaggtcag tgtggtagag agagttggat
     4621 atggcgtcca gattaccgca caaggtaacg agaaatcttg cttagtaacc tatgaagcaa
     4681 tgttaggtat ggcagaagca atgggattat ttgatgaagt taaggagcaa aataatgact
     4741 aaaaaaacaa ccagagtaaa aagtgcaacc caaagtgcga tttatcaaag ccgagatgaa
     4801 gtgcaagtgg ctataaaaat cattggtgat aaacagcgtg aattgcaacg tttagctacg
     4861 gcaatgaatg atgaattagc tgctattagt gccagttatg ctcaacataa ccgctaccaa
     4921 cattatgccg agaaagcggc tgaagcagaa cgcaaaggca actataagga agctcaagac
     4981 cattgggaag ttgcaaaatt atcagcaaaa aagaccgcta accgagactg ggcggaacaa
     5041 cgagcggaat tttgtaaacg tatgcacaat aaaccgtttt agggggaagt gatgacagaa
     5101 accgtaaaag cacaacttaa cagccaatta aatgaggcaa ttatccagtt gatacaagct
     5161 caaaagtatt taaaccaaga tgatgccatt cgcagtggag tgtatatcgg tacggtgcag
     5221 gatttattgc caaaggtgca tttaaagtta ttaacggcaa atcgtaaaca ttgatagaga
     5281 tgaggtaaaa atggctatta cagaagaaca atggaaagaa attaagcaaa agctagatag
     5341 tatcataggg agagttaaat ttagatacaa agagcattta ctcacggtag atgttgtaca
     5401 aattaagcga tctttaaaat tggctgtgta tgttgatggg gaaattgatg gagcttggac
     5461 aaaagaagga cacgaaattc gcccttattt agaagaagtt tggtatcgca aagaacgccc
     5521 tttttttaat gctaaggaga agaaagagta tagaggttta atgagcaaaa aaaaactgaa
     5581 tgaaaaaata gtaacctact cacctatgtt tccatcacca actgcactga ctcgtcaata
     5641 taagaaacta gatggcttag agttgattga agtgatttaa aacccattta cagcccattt
     5701 aaccccgagt tatttaaagg acaacaaaat gaataaaaac gaatttttta agaaatacca
     5761 cctagagggt tcactagcta ttttggattt ggatgcaaat gacgatcaaa gccaattctg
     5821 ttaccaagca ttgttggagt atagagaact cccgttagac acatttcttg cagagtactc
     5881 agatgatgag gatatgacag agtcagagtt tattgagtta atgcttgatg aattagagtg
     5941 gattatccgc agagctaaga atgctaaacg gtttgttaaa aaacgacaaa aaacgatgag
     6001 ggaggaaatt atgtaacagc tcaacccaac caacgccaca tcatccgtct agctgagaaa
     6061 caggtgtggc gtttgtttta agtgggcaat caaaagcgat aacgttttta attgtttatt
     6121 tatcggagta acaaatgaca gacaaagcca agctaatcca gcttatccat attgccaagt
     6181 cgcaactagc aatggacgat ttaagctacc gtgaaatgct gaaacggctc accaataaaa
     6241 ccagctcgac caaatgcacg gtggtcgagt tgcacaaagt cttacacgaa ctgcaaacca
     6301 aaggagcaaa agtaaaatat tttgccaaac gcagcaaaaa accgaccgct tacagcccgg
     6361 caacagggga aacagcggta aaaagccaaa ttgctcataa aatccgagcg gtatggataa
     6421 caatgggcaa acacggattt ttaaacgacc caagcgaaaa agcattaaat gcttatatgc
     6481 gtaaggttat caataataaa actagaccaa caatattatt aaatgtctct gcattagagg
     6541 ctggagatgc aagttatatg cttgaaatcc tcaaaaaatg gcataaacgt gtgatgatta
     6601 aagccctaag caaaagttgc acagttgatc caaagataag ctacgatgaa ttagtggagt
     6661 ggtacaatga agttatgtag atgcccgatt tgccacagtg atatacacct agaagctctc
     6721 gtagaagatg aagccgggcg tgaactactc ggcaaaatca gccaattaac acacggtgtt
     6781 gctcaaccga tggttgctta tttaggctta tttaaaccgc aaaaaagtaa ccttaataac
     6841 tcaagagcct tgaaaatact caatgatgta ttagcactct acccttgctc attgctactg
     6901 gctcaagccc tatctgaaac ggtagtaagc atacgcaaaa aacgtcagca agcaatggaa
     6961 aacgggcaga aaattgaacc gctaccgaac cataactatt taaaatcggt gtatgaaacg
     7021 caaaaagtcc attttgctgt ggtgagaacc ggcaaaaccg aacgcgaaac aatgaaagcc
     7081 gaactggaaa aaacacgcaa tgcgattgcc tatgtgcaac ggtttgttga tttaggacgt
     7141 gaagaggttg ttaaaaatag ccctgagtat cagatttggt tagctcataa acccaaataa
     7201 cacaagccac caaaaggtgg ctttttaact ttttgaaagt gcgataaaac ctgatttaaa
     7261 caggctttaa acgggtttta agattgagtt taaaaaaata tttcacaaaa taagaatggt
     7321 tttttataca ataaccgaaa cggtttttgg ataggaaata gacaatggaa ttagtggata
     7381 tttttgaaga aaaagccccg gaagtgttaa ccgatttagc caaacatatt gaagtcgcat
     7441 tagaagaaaa atgccaattt agccccgaaa aggcaaagca aatcggtatt gatattgccc
     7501 aacaaatggc tcaaaactgg ggtggcgagg taatttatat tcctcgcaat ttgttgatgg
     7561 ctcttaatga gcgagatatg aaaatttacg aagagtttaa cggtaacaat caccgtgaat
     7621 tggctcgtaa gtacaatgta tcaatgcagt gggtatataa aattgtgaag aaagtccata
     7681 aggacgaagt tgcccgcagg cagatgagta tgtttgagat gtaaatcatt ttttcccagc
     7741 cctgatcatt ttcagggctt tttgtgtatt aaaagaacgc agtctttaaa cccgtttaaa
     7801 atcaattttt cagcaaattc tttaaactcc ttttaaagca attttaatag gagtgtgcta
     7861 tgtctcaaaa tcttaccttt cgccaaattt ttgaccgttt aatcggacac gagggcggct
     7921 atgtcaatca cccacaagac ccgggcggag aaacccactg gggaattacc aaacgtaccg
     7981 cggttgctaa tggttatatg tatgcaatga agaatatgac ccgtgagcaa gcctaccaaa
     8041 tctacgaaaa agccttctgg caacgttatc gttgtgcaga gcttaagcca gcagttgctt
     8101 atcaattttt cgacgcagtg gttaatcacg gttttggtaa tgctagtcgt atgttacagc
     8161 gtgcagtagg cgtagcggat gatggcatta ttggcaatat taccctgcag gcaattaatg
     8221 caaaatctga aagcgatctc ttaatgctat ttaatgccga acgtctgaag ttttacacaa
     8281 aattacgcaa cttccccact tttggtaagg gttgggtcaa tcgtgtggca ggcaatttag
     8341 aatacgcagc acaagataat gaggtttaaa aatggcaaaa ctatttaaag aactactctc
     8401 aaccaatggc acattatcca ccactagcac cgtgcagttt tttggcacat tgtgtgtatt
     8461 tggtttaatg gtttatgcag taattaccca acaaccttat gccgaaagcc tattaaataa
     8521 tgtgttgatt tatctttttg gggcaactac cgctaagggt gtagtaacca gctatcaagc
     8581 caaaattaaa gggggtatcc ataaacaaca aagtacattt aaagaggagg agggatgtta
     8641 atgcaacaaa ttttaggcgt gattggcgtc atcgcagcaa tattgtctta tgctttgttt
     8701 aaaagctggc aactaaaacg tgagcgtaaa aagaatgcca tattacaagc tgaaaaacag
     8761 caacaagcgg ttgaaattga gcaaaaaaaa gcagaggtaa aatatgtgta tatatcaaag
     8821 aaaaacaatg aaaaagttac tcgcagctct gctagcgata ttgatgacaa gttgcagcaa
     8881 cacggttact tccgtgacaa caacaggttg cacggcgttc agtctgattt atccgaaccg
     8941 tgcggatacg ctggaaacga aacgtcagat tcttgctcat aatctgactt ttgaggaaat
     9001 ttgccaaaat gaaatacatt aaaaaattac gtaaacgctg gcaaatttgg cgttttttaa
     9061 aaaatcatcc tgaggttgca caacgccgag atttattagc gattgccatt aaacagggcg
     9121 tagaaaaccc tgtagggcgt gcaagacgga gggagccata atggatttat ttacagtctt
     9181 ttgggagtct atccgtgcta actttggttt tatttctgcg ttactcacca ttgttgttac
     9241 ggcattttgg ctcaagctag atagcaaata cgccaagaaa aatgatcttg gtgaattgct
     9301 ggaaattgcc aaaagccacg ataaccgctt aaccacctta gaaaccaagg tagaaaacct
     9361 gccaagtgcg gtggacgtgg aaaagctgaa aaccttagtc actgatgtaa aaggcgatac
     9421 caaagccacc tcaaagcagg tggatagtat cagccaccaa ctcggcttgt tattagaagc
     9481 aaaattaaag gaatgatatg tcgttaagag atttactcac tcaagaccaa cggctggtga
     9541 ttttacgctc attagcggaa gccggctatg atgccaatga gtcaattatt agtgattgcc
     9601 tagatttata cggtcacgat attagccgtg atttagttcg cacccattta agttggcttg
     9661 aagagcaagg cttacttacc ttagaacgtt tgcgagatgg ctatatggtg gcaagcatta
     9721 cccagcgtgg cttagatgtt gctcaagggc gtgttgtggt aagtggcgtt aaacgccctc
     9781 gccctaaaat ttaaacggta tttaaggagc gtttaaatga gcgagaaaaa tactcgtggt
     9841 cgagcatcaa aagtcgattt actgccgccg aatattaaaa cccaactggc gatgatgttg
     9901 cgtgataagc agtactctca gtcacaaatt ttagaagaaa ttaatgactt aattcgggat
     9961 tgtggcttgc ctgatgaaat gctgttaagc aaaaccggat taaaccgcta cgcaagccgt
    10021 atggaacgct taggggcaaa aatccgtaat gcccgggaag ttgccgaaat ttggactaag
    10081 caattcggcg aaatgccaca aaccgatatt ggcaaaaccc taatggaaat ggtcaagcaa
    10141 attgcctttg aaacctcatt gaaattaggc gaacaagaag gcggtattga gccaaaacag
    10201 cttgctttgc tctcgtctgc tattcaacgt ttagaacaag ccgaaagttt gagttttaaa
    10261 cgggaacagg caatccgtaa agaggtcgca caacaagccg ctgaaaccgc tgaaaaagtg
    10321 gtggtgcaag cgggtttatc cgctgaaacc gtccgtacga ttaaagagca aattttagga
    10381 attgcataat ggcactactc aataacagac cgctgaatga attagcccct gaatgccgtt
    10441 cgtttttaga gagcattcac gcttttaatc ctaacgaact gttattgggc tatcaaaagc
    10501 ggtggattgc ggacgacagc caactcaaaa ttgccgaaaa aacccgccgt tgcggtttaa
    10561 cgtgggcaga agcagcagat aacgccttaa tcgcaagcac ccgcaaatca gacggtggct
    10621 ctgatgtatt ttacattggc tcaaataaag agatggcacg tgaatatatc gatgctgtcg
    10681 caatgtgggc aaaagccttt aatcgtgtgg ctggagaaat ccaagaagag atctttgaag
    10741 acgaagataa agacatctta acctatgtaa tctacttctc atcgggtttt aaggtcaagg
    10801 cattatccag taatccgaaa aacctgcgtg gtatgcaggg cgtggtggtg attgatgagg
    10861 cggcattcca cgaatactta gcggaagtct tgaaggcggc gttagctctc acaatgtggg
    10921 gggcgaaagt ccgtattatt tcgacccaca acggctcgga aaatctgttt aatgaactga
    10981 ttaccgatag ccgtgccggc cgcaaacgct actctgtgca tacgattacg attgaagatg
    11041 cctgccgtga tgggctatat caacgtatct gccaagtagt gacgaaaaaa acgtggtcac
    11101 cggaagcaga aaaagagtgg attgaaaacc tactcaacga caccgccagc gaagaagacg
    11161 cattggaaga atacttctgc gtaccgaagt tcggctcagg cttatggctc tctcgtgctt
    11221 tgattgaacg gcagatgagc gaagccacgc ctgttatccg ctttacggca aaagatggtt
    11281 tcagccttgt ggctgaaccg acacgctatg cggagattgc cgaatggtgt gaattagaac
    11341 ttgagccgat attggcaacc cttaaccctg atttgttgca ttttatgggc gaagactttg
    11401 cacgcagcgg cgatatgacc tctctggtgg tgcttgctca gcaacaaaac cttgttcgta
    11461 aagtcgcctt tattgttgag cttggcaata tgccctacaa gcaacaagag caaatcgtga
    11521 tgtttatgct taaacgctta ccgcattttg ccggtggggc ttttgacgcc cgaggcaatg
    11581 gtggctattt agccgaagct gcacgagatg aatttagcac cttgattgac tgcgtgcagt
    11641 tatcggaaaa atggtatcgg gaacacaccg ccccctttaa agcggcattg gaagacggcg
    11701 agctggaagc tattccaaaa gatgcggata ttttggctga tttgcgttca tttcaggtgg
    11761 tgaaaggcgt accacgcata ccggataaac gcactaaaag tgcagatggg aaaagcaaac
    11821 gacacggtga taccgcaatt tctttgctgt tggctcatta tgccagccga caattagtgc
    11881 aaatgccagt tgagccggta tcacgccgtc cacgccatag ccaacaatta acagaaggat
    11941 attaacaaat gagatatgta ccactttgtt taatgatgtg tgcctcattc tacctattta
    12001 ttaacaacat tgacggctgg ggttggggaa ttcttttatc ttttatttta gcgagtgcag
    12061 cttatggcaa agaagaataa tttaattagt gaaattgcca cccgtgccag aagtattgat
    12121 tactgggcat ttggctatta tctgccgaac cctgacccga ttttaaaacg aatggggaaa
    12181 gatatttccg tttaccgtga gttgttatca gacgggcaag ttcgctcagg cgtacgccgt
    12241 cgaaaagcgg cgattaaagg cttagagtgg cgaattacca ccactaacaa tgagaaagtg
    12301 gacgagcaga tttatcaaat atttaaccgc ttgccgatga ataacctgat taccgaaatg
    12361 ctcaatgcag cactctatgg ctaccatatc agcgaggtta tttgggcaaa agagggtgag
    12421 ttatttgtgc caacagaaat cattggtaaa aagcctgaat ggtttatgtt tgacgacgaa
    12481 aacctactgc gtttccgtac caaagaaaac tgggcggacg gcgaattatt accagatgat
    12541 aagttcttgc ttactacgca agaagccacc caagataatc cctacggttt aggcgattta
    12601 tccctctgct tttgggcggc gacctttaaa aaaggcggtt tcaaatactg gttggaattt
    12661 accgaaaaat acggctcacc gtggttgatt ggcaaacacc ctcgccaagc tcacaataca
    12721 gataaagagc gactagctga tgccctagag ggaatgatta gctcagcgat tgcggttatc
    12781 cctgaagata gttcggtcga aatcaaagat gcagcgggca aatctgccag caccgatgcc
    12841 tttgaaaagt tcctcaattt ttgcaaggca gagatcaata tcgccctact tgggcaaaac
    12901 caaaccaccg agcaagaaag caaccgtgcc agtgcacaag taggcttgga agttatggaa
    12961 gatattcgct cggacgacca agcgatgatt gaagccacct tcaacacctt gttgcagtgg
    13021 attgtgaaat ataacttcaa tgtggaacaa ctgccgaagt ttgaatttta cgagcaagaa
    13081 gccatcaaca ccgaccaagt tgaacgagac agtaaactcc acgcaatggg cgtgcgtttt
    13141 tccaaagcct actttgagcg tgaatatggc tttgaggcgg gtgatattga ggtgcaagcg
    13201 gtcgaaaaag cggaaaaatc tgcaaatacc acagaattta gcgaaggaca acgccgaacc
    13261 caagtgccaa tgcctgtgcc aattttggaa cagctagaac acgagggaga agcaatggtg
    13321 gacgattggc tacaaggggt gagagataaa ctcgctcaag cagatagtct ggaggatttc
    13381 caaagtcagc tagatagcct tatccctgaa ttaagctttg ccgaatatgg cgagctgttg
    13441 gcgttaggct caacggtggc agagcttgca ggcagacaat cggtaaacga tgaaattaag
    13501 gcaaaacgca atgcgtaaat ttacctttaa acagcaagcc caatattttg agaaaaaact
    13561 caatctccgc acgaatagct accttgatat tctgggcgaa gagcacgact attttttcgt
    13621 ggtggctggg gcgaatcgta atgaagtgtt gagtgagttt cgcaaggcgg tggatgaggc
    13681 tattcacaac ggcgaaacct tagagaactt tcgcaagcgg tttgatgagg ttgtggcaaa
    13741 aacaggctgg gagtacaacg gcggacgaaa ttggcgaaca cggattattt acgataccaa
    13801 tgtttacggg gcgtataacc gtggacgttt gcaacaacat ttagagcttg cggaggatat
    13861 gccttattgg gaatatcaac acaacgacaa cgcccaccct cgcccacagc acgtggcgtg
    13921 ggacggcttg gtgctgcgtt atgacgatcc gttttggaaa tatcactatc caataaaagc
    13981 ctacggttgc cactgcaccg tgcaagcgtt agatgatttt gacctcaagc acgatggtaa
    14041 aaccgtcagc ccaccacctg aaattgagtt tgaacaaaaa acggtaggcg tgcggacagg
    14101 caattcacga gtcgtaactg tgccgaaagg ggttgatgtg ggttttacac cgcataattt
    14161 tgataatctc aaagccggga gaacagcaaa tgtagataag ttgctaatgc aaaaaatggt
    14221 taccgccgat cctatatttg cctccaaagc agtcagtgat ttattggcaa aagagccggt
    14281 tgcaaagtta cataaccaag cctttaaaaa ttgggtaaat gatgttgcca atgctaaagg
    14341 aagtgcatta tcccaagtag agaatatgca gttgttaggg gtactacctg ttgcggtgat
    14401 tgaacggtta cagcagatca atttagctcc gcaatctgca attattgcaa taacaaaagg
    14461 cgatatggta cacgctctac gagagctaaa acaaaatacc ggtattgccg taccgttgga
    14521 attttgggaa aagttgcctg caaagttaca atacccaaat gccatcttgt tggaaaaaca
    14581 acaagcagaa actacattgc tctttatcta tgacgtagct caaaccaaaa ttgccgtaaa
    14641 agtcaattat gatgcaactt tacaagatag acacggagca aaaggacggc agaaagtctt
    14701 actgaatcga gcggttacag gtagtttatt ccaagataca actggattga aaagttcaaa
    14761 gtatgaagtg ctattgggag aaatgtagcg gcgaaggact cgaaccttat aatgtaggtt
    14821 aaacctacaa cctttaccaa ttaggaaact accgctacat tgtgtaaatt cactataccc
    14881 ctaaattatt ttttaatcaa taggagaaac aaaatggcag aaaaacaact ttcctttaaa
    14941 gtagccgaaa tagtgccaat gtcagctatt aacactatgc ggcacttaat tagtttggca
    15001 ttacaagcga attatgagag agaaaaatct cgtgcttgat aatttcaatg gcttgtagtt
    15061 cgggaactat attatctgaa acaagccatt gttttgttga tgtcagacat ttagcgatga
    15121 aatccataat taaaccatta gcagtagctc gttgttccgg agtaagttgt aaccacgacg
    15181 cctctgtttg ttggtctaat gtataaagaa acataacggc ttctttttct gataatgggt
    15241 caagtggtaa tttttcagct tttgtgacta accattgacg ggaaagcaat aaatcataaa
    15301 ccagttctat tgcttgttct gcatttacca tcatattcta attccttaat atatcagggc
    15361 aacattaccc aatctatatt ttactgagat ttgcttaatg atcaaaatca ccctcaacaa
    15421 cacccaagcg gtcaaatcgt tacaacaaat tgcaaatcag ctggaacaac ctcgccgact
    15481 gtatggcatt ttaggggaaa ccctaaagaa aattcacact gcacgcttta aagcggaaca
    15541 agaccccgat ggcaacaatt ggaaaccgtt agccagtagc acgcttgctt taaaacgtaa
    15601 acgaggtaag tccactaaaa tcttacggca agacggctac cttgccgata aaacggctta
    15661 caatgttaca agtgataatg ttgagtttgg ctcgcaggaa gtgtatgcac ggttgcatca
    15721 attcggcggt aaagccggac gaggcaaaaa agtgactgta ccggctcgcc cgtggttagg
    15781 cgtaggcgat aaagatgagc agttactttt acgcaaagct gagtggcatt tagggcaaat
    15841 tatcggtcgt atgggaaaat aaccgcttaa aatcaaattt aacgcataaa aacgcctctg
    15901 tgcgttttta ttttttattc gcctgattat tcgactcaaa aaatttaaac gctgttaaac
    15961 gcatttaaac ggtatttaaa cggtattgcc gattgtgata aatcctcttt attaaaacac
    16021 cctcgaaaaa tctttaaacc agtttaaaag ctaatttctc tccaaatcct tattattgcc
    16081 ccatcacgga ggcaatatga cccaaattga aatttttaaa gccggtaagc gttatgacgc
    16141 caacggcacg ttaattgaca ttacccctga aatgttgcaa cagaccgttg ctgcctataa
    16201 ccccgaattt cacgaagctc cgctggtgat cggacacccg aaatccaaca accctgcgtg
    16261 ggggtgggta aaagcccttt cccttgaggg cgatgtacta aaagcagaag tagatcagct
    16321 agacgctgag ttttcagaaa tggtggcaag cggcaaattc aaaaaagtct cggctgcttt
    16381 ttacttacct gatagcccga ataacccaca taaaggcgtg ttatcgctac gccacgtggg
    16441 gtttttaggg gcaatgccac cggcggttaa aggcttaaag caggtggaat ttgcagaaga
    16501 tgatgatttt gtcgtctttt ctgaatgggg ggttgcaagt ctatttagtc gttttcgtga
    16561 ttggttggtg agtgagttcg ggcttgaaaa agccgataaa gctcttcccc cacacgaaat
    16621 tgattggctg aaagaagagg caatgcgaag ccaaattagc aaacaggtac acgccgaaca
    16681 ggcaacacct gaaccgattt ttaatgaacc cgcacaacca gaaggagaac ctgaaatgag
    16741 tgcagaagag aaggaagagc ttgaacgctt aaaagcggaa aacgagcagt tgaaagccga
    16801 aaaagcccaa gccgaagcag agaaaatgga agcagagctg gaagctgaaa aagctggtaa
    16861 tgcagagttt tgtgaaggtt tagtcaagca aggcaaactt gccccggtgg caaaagcggc
    16921 gtttgtgcag gctttaaata acctttcagt attaaaagcc ggtcgtgagc cggaatttaa
    16981 tgagggcgaa gatgtggttt cacagtttaa gtctgcaatg tcgcaatcac cacagattat
    17041 ccaatttgga gaatatgcca cagcagataa agccaaccca caagaagcgg aagcagtgga
    17101 atatgctgaa ggcacagatc cagtttcgat tgaagccgac caaaaagtgc gagcttatat
    17161 gaaagcccac aatgtaggct acgctgcggc atttaatgcg atttatcgct aatgtttaac
    17221 ccacaactga aaaggaatac taatgacaac tcataactta caaaaactgc gtgtacaaga
    17281 ccccgtttta accgagctgg cacaaggcta tcacaataac gagctggttg ccgagacctt
    17341 aatgccgact gtcgaaatcg aaaaggaagc aggcaaaatc ccaactttcg ggcgtttagc
    17401 tttccgattg ccttctaccg tgcgtaactt gcgtggttca tctaaccgtt tagatccaga
    17461 agatattggg gcgattgatg tggcgttaga agaacacgat gtggaatatg ccattgatta
    17521 tcgtgaagag aacgaagcca ttttttcttt acgtcaattt gccttaaata ccacccaaga
    17581 tgtgattgcg ttaggtcgtg aaaaagcggt tgcaaccctc gccttagatg aaagccgtta
    17641 tgaaacagac aataaagtca cgctgtccgg caccagtcaa ttcagccata aagatgctga
    17701 tgtgtttaag gtatttgata ccgctattcg tgccgtgaag cgggctatcg gtcgtaaacc
    17761 gaatgtgtgt gtgatttccg gtgatgtgtg ggcagcgtta aaagaacatc cggcagtggt
    17821 tgaaaagctc aaatacagtc aagtagcgat tgttacccct gaggtgtttg ccaaactgat
    17881 cggcattgag accgtaaaaa tcggtgaagc ggtgtatgaa gaaagcggtc agctcaaaga
    17941 tatttggagc aaagatattg tggtggcgta tgttgctcct cgttcgaccg gaggcaaagg
    18001 cacggtttac gagccgtctt atggttacac ggttcgccgt caaaaaggct tatttgttga
    18061 tacttataaa gagagtggcg gcaaaattga agtggtacgc acgaccgata ttcacaaacc
    18121 tcatttagtg ggtgcggcag caggctattt aattaaagct gccgtataag ccacatacca
    18181 tagggtgcgt ttactaacgc accattatct caaacacatt acatagagga aaaaacgatg
    18241 gctaaattta ttgttttaca taccccaatt ttacacgatg gcaaacgcta tgaggtgggg
    18301 gcagaaattg aactcaccgc tgaacaagct gagaacaatg cgatcaactt aaaagcggta
    18361 gaagctgttc caaaaccacc taaaaatgcc tcaaaacctg aagatgagcc aaaacccgaa
    18421 ggtgaaccta agcctgaaga tgcagtgacg gaagatgatg tgaagctggc tgaagcaaaa
    18481 attgcagaag cagagcaacc aaaagggaat aaaaaataat gtatgtgcag attgatgagc
    18541 tactcacggc gtttagtcgc aagattgtgg tgcagctcag taatgatgaa ccgacagcga
    18601 ccgagcctga tttaaagatg cttgaaacgg cagttaaagt ggcaaatgag cgaattgatg
    18661 cagcgttacg ctctcgctat accttgccgc tggctgatgt gcctacgctt attaatcagc
    18721 acgcattaac ccttgcccgt tattggcttt atgcccgccg tcctgaaacc aaaatgccgg
    18781 acaccgtgaa ggaaacctac caacaagcgg tgaaagagtt ggaacagatt gcaacaggca
    18841 aactgcattt aggcattgct gaaagcagtc aaattttaga agacgatgtt ttagccgata
    18901 acagtgaata tgaagttcgg gcgaacgctc gcattgatac cagtcgttat taaagattat
    18961 gtcagccaca caaccgattt tagaagatat ttgccaacac ttgcggcaag cattgcccga
    19021 gtgggaggtg gaactcttcc cgaatgaccc aagcacctat catctctcgc acattaacgg
    19081 tgcggtgttg attagctatt tggcaagcaa atttgagaaa cctcgcacta cacaagcggt
    19141 gctgcaggtt cgccacgttc agattgcttt aacggtgctg acccgtgatt tgcataatga
    19201 tgtcggggcg ttggatttgc tggataaatt gcggttattg gttgtggggt ttaagcctca
    19261 aaactgcggt gagtgttggt tgattgatga attttttaat ggctcaagcg aagaaaccgg
    19321 gctttggcaa tatcagttga ttttgcaaac cgaaactcag caggtgcaac accaagcggt
    19381 cgaaaatctg ccaaaatttg taaccgctca tcttcgccgt caagaccagc ctggttcgcc
    19441 cggacttaaa acttaaatcg tagggtgcgt ttgctaacgc accatcaaca tcacctgatt
    19501 ttacggtgcg ttggcaaacg caccctacaa ggagaatatt atgacgtttc accacggttc
    19561 tgaaaccgaa cgagtaaacg gtggctctgt gccggtatcg gtggttgata gtgccattat
    19621 cggcattgtc ggtactgccc caatcggcgc agttaatgaa ttgaccgtct gtttaaccaa
    19681 aaaagatttt gccaaattcg gcacgatttt aaaccaaggc ttcaccttac ctgatgcctt
    19741 tgatatttta gctcgttacg cagcgggtaa agtgtatgtg gtgaatgtgc ttgacccttc
    19801 ccgccaccgt actaccgtga ccgatgaagt cctcacactg gatcgcaata cgctgaccgc
    19861 tcaaacagcg aaaaccggca ttattgcggt gacgagagtg aaagacggtg caaacgtgct
    19921 gaatgctgat cggtattcgg tcgatctgca aaccggcgtg gtgacctttg tctctgcacc
    19981 gtctgccccg aaaattagct atatctatgc tgacccgagc aaagtgaccg aagaggacat
    20041 taaaggcggg gtggatagct taaccggtaa acgcaaaggt tttgaactat tgcgtgatgg
    20101 ttttaacctt tacggtgcgg atgccaaaat cttaatctgt ccggaatttg ataaaaccgc
    20161 aagctgtgcc gctgcccttg ccacccttgc cgaccaactg cacgctaagg cgtatattca
    20221 actgccgaaa gggacgagct tatcaaaagc tattcaagga cgtggcccgg cagggacgat
    20281 taacgcctcg gcaagcaatg agaacgtacg ccatttcttc ccgtatgcgt tggggtcaag
    20341 caatactctt gaaagccttg cgacccacgc cgccggtttg cgtatgttga ttgatgtgga
    20401 gaagggctac tggttcagca cttccaataa agagctgaaa ggggtgatcg ggttggaaat
    20461 tccgctgacg gctcgggtgg acgataagca aagcgaaact aatctgctca atgcggtagg
    20521 gattaccacc attttcaata gttttggcac aggcttccgc ttatggggca accgttcgtc
    20581 taacttcccg actgtgaccc atatcagcaa ctttgaagtc gcctctcgca caggcgatat
    20641 tattgatgaa agcattcgcc aagccgaatt gcagtttatt gatttaccga ttgatgatgc
    20701 gttgctggat agcttccttg aaaccatcga cactttcctg cgtacgcaga aatcgttggt
    20761 aggttattcg gtgagcttag actatgaata tgacttggtt gatgcattca gccgtgggca
    20821 agtcccgatt gtgtatgaat acactcctaa aattccgggt gaacgtatca gcaataaatc
    20881 tgtgatgacc cgtaaatact tggcaaactt ggtgtcgcaa cgataagggg aaataaatga
    20941 gtattgcaat taaccaaatt gtaaacggaa atgtttacat taacggcaac agccaaatgg
    21001 ggcgagtgaa tgaggtcaaa atccccgata ttgagtttga atttatcgaa cacaaaggcc
    21061 ttggaatgca cggtgtgatt aaattacctg ccggcacgaa tgcgattgaa gcagaaatca
    21121 cttgggacag tttttatccg gaagtgcgtg cggtaatgct taatccgtat aaatacactc
    21181 agttgcaaat ccgctctaac ctgcaagtgt ttaactcgca gggcttggca gccgaagagc
    21241 cgatggtcac cattatgaat gtgtctgccg gtaaaatcgg cggcacaggg cataaaaaca
    21301 aagaaaatgc cgaatttgcc gacacgttaa atgtacattc aattaaacag accgttgccg
    21361 gtcgtgagct gttatttatt gatgtgctgg cgaatatcta ccgtgtgaac ggggtggatg
    21421 tgttgcaaaa attccgcacc aatatcggtt agtaaaatct ttaaagccgt ttaaaatcaa
    21481 tttaaacacc gtttaagtaa actcctttgt gaagttaaac aacaaccact cacaaaggag
    21541 tttttttatg gctaacgcag ctcaacaagt attaaccaat cttcgtgtac aaaccactta
    21601 taaattgcaa taccctatcg ctttaccaga tggtagagtt attgaaacct tagatgtacg
    21661 tcgtccaaaa ggtaaagatt ttcgtttgct tgatgaaaaa gggtttgacc ctgaaaaaga
    21721 tggcgtaaaa attctacgct tctatattca acagctcacc acacttgtgc cggaagatat
    21781 tgatgaactc gatgctgccg atattaaggg cttaaataac ctattggaag atatgcttat
    21841 tgagggaaag tccgacaaat aagtgaacta gataccgttt atgctgatct cgcttggtgg
    21901 tttaagtttt caccaagcga gcttttagaa ttggatttag tggatattcc gaaatgggta
    21961 gaacagatga acaggcaaat aaaagccggt tatgggcaga ttttacggta gagccaaata
    22021 aagggttcgg tggctaaagt aagtaaaatg gcgacgcccc aagctagata cacaccaacc
    22081 cacgccaaga ttgtcaaaat caatccataa atgagtacaa aactaacgcc ctcgcttagg
    22141 ctttgtatag attgaatcgc atctaaccag tagaagatac tccaaccgac taatgctacc
    22201 gctaacaacc atttagcggt gcattctgtt cttgttacac aagcctcgaa accagcccac
    22261 tcagtaattc gattatttaa tttagtcagc atacaccacc tccttaaact ttccctattt
    22321 tacgctaagg atttaaccaa tggcaaataa tttagctatc ggaatggtaa ttggtgcaag
    22381 cctatcatca ggttttcacg gtgctttttc aggagcaaag aaaaagctag aacaattaga
    22441 acaggctgtc gctaaaaatc agcaaagcca ttctaaatta ggttctgagc ttactgcatt
    22501 gcgtagtaaa caagctactc tctatgcaga aatgagccga gccagtagta aaggtggtgc
    22561 tgggcttgct cttatgcaac aggaatataa aaaaattggc aaagaaattg ccaatattag
    22621 ccgagagcaa cgcaaatgga gtacagaatt agaaaaatct attgctaagc aaactaaatt
    22681 acgcaatgca atggaacgtc atacacaagc taaagaaaac cgagacgagc ttaaagggaa
    22741 aataattgga acggtagctt ctgcggcaat gggtgtcggc gtgatgaaaa cctatatgga
    22801 gcaagaagaa gctgcgaata atcttaaaat ttcaatgctg aaagcagatg gttctttcgg
    22861 gaaatttgaa gagattggca agattgctga cacattaggg acagatttac cgggaacaaa
    22921 aaaagatttc tacaagctcg caatggcatt aaagaaacaa ggtgtttctg atgatgtgct
    22981 gaccggaggg gcattaaaaa cttctgccga actgaatgtc ttgcttgata tggatcagga
    23041 aagtggtgga gagtttttag cgaagtttat ggaatctcac cggctcaatg aaaatgagtt
    23101 accacaagca gccgattatc tgcaacgagc aatgtttgcc ggcggtttaa gtaaagagca
    23161 gatgtatgaa tcgatgaaat actatgctcc aaaactgaac tcaatgaaac ttacaggggc
    23221 tgagaatacc gaaaaagtgc tggcgattga agcaatggca ggtcagcaag gcttagaggg
    23281 ttcaaccttt ggtacgggct taaatatgat gttaagccgt atgaataaag gaccggaaat
    23341 gctcaaggca gcagccaaag gaatgaaagc cgaagctcga gagatgatgg aggctgtcgg
    23401 agtagaattt aatttctggg ataaaaacgg ctcattcaaa ggcattgatg gaatgctggc
    23461 tgaaatgacg aaatttgaaa aaatccgcaa aaaattcggt gacaaaggtg taggtttagt
    23521 tgcagaagag ctgtttggca ttgaaggtgg gcgtcttgct gatattttgg ctatgaaagg
    23581 tcaaaaaggc ttagatgaaa tgctgagtaa aatgcgtgaa caagccagcc ttcaagatcg
    23641 cattaacctt aaaacaaata cattaggtgc tgccctagaa tctttaggag gagcgtggga
    23701 aacctttgtg ggggatattg gctctgtctt tgccgaagat ataaaagggt tttcaaaagg
    23761 cttgcaatct gcggtggaat ggcttgtgcc atttgtatca aaacataaag aggcgattaa
    23821 atttgtaggt ggtttggttg ctggacttgt tattttacga ggcgttttac aagctggttc
    23881 ttttgttatt caaacggtaa ctatggcaat gaacgccctc aaaattgcag caatgaccaa
    23941 tcctattgtc ttagtcattg gagcaatcgc agcgggtgct tacttgattt atagccattg
    24001 ggatagcatt gccccttggt ttgcaaatct ttggcaaaat gtgaccgctt acttcaatca
    24061 attttggact tgggtaaaag gtgtttggtc gggcgtgacg gactgggtgt cttctgtgtg
    24121 gaatggtgta gccacttttt tcgggcagtt atggactacg attactagct tatttagtgt
    24181 agcttggaca aatatccaaa caatatgggg aggagcaaca gcttggttct ctggtttatg
    24241 ggatgggatt aagtctgcgg cttttggtat gttcgacagt attagccaaa aagtcagctc
    24301 tgttgtagca agcattaaga gctttttagg ctttggcgat gatgtggata aaatgggggc
    24361 aaaaattggc aatgtagcta cccaagtcat cagcaaagaa cactcaggac aactcagcaa
    24421 aaccgcagat atggcagcca atacagggtt ctctagcggt ggtttcaccg ggcaaggcgg
    24481 taaatatacc cctgccggta ttgtgcataa gggcgaatat gtgatgacga aagaagcgac
    24541 ctctcgtttg ggtgttggaa tgctggatag cttaaactat ggcggtgcta tgggtttcac
    24601 tcaacctgtt ttgcaaccga ttttaggtaa gcaaaaaggc ttttttggct cactttggga
    24661 cgatgtgaaa ttcggggcaa actttgtcgg caatctgctt ggcttaaatc aaggcagcct
    24721 gaaaacgcct gattttaacc caaatgcgac caatgcaagc ggtcaaaatt cgtcgatttt
    24781 tagcaattat gaaccactca accgaaatgc ggtaatacag caggaaagct cgcaagcacc
    24841 gcagatagtc gtgaacttta acccgacaat caatgtaaac ggtaatgcta cgcaagaggc
    24901 tcaatcgcaa attatgcagc agttgcaaat gagtgcttat gagtttgaga aattgctcaa
    24961 tcgtgtgcta gaccaacgaa gccgtagagc ctattaggag tgatttatgt attttatgtt
    25021 aggcaatatt gcctttgagc cggtgaattt aactgaattt agcgagcaac ataccgccga
    25081 atttgccgaa cacgctgtgc taaaaggcaa acctcgttta caagcgatgg gcgaaaagct
    25141 aaatgaactt aatttttctc ttcggttgca ccataccata ggcggtgtag agagccgtta
    25201 tcaagccttg cttgaagcca aagcgaagca agcggcattg gcgttaattt ggggcagtcg
    25261 ttataaggga gattatgtaa ttgtcgatat ttcatcgacc acattgttca ccgatggcag
    25321 aggcaatgcc ctcgctcgtg aaatgcaaat cagcctgaaa gagtttgtgg gcgataatga
    25381 cggtggtgtg ttgggtgagg cgttaaattt tggtggtggc tcactgcttg gctctatttt
    25441 gccaagcggt gcggtaacta ccctatctca aatcaaaaca gcagtcagcc gaggtgtgga
    25501 gctttataac agcggcaaac gcttagttga cgaagtgcaa aataccgtgg cgattattcg
    25561 gcagttgaaa aatgacccgg caactgcatt ggcatattta cccgctgcat tagcgaattt
    25621 agacggggca ttggggcatt ttggtaattt gaccggctca tcggcgacct tgggcggttt
    25681 gcaggagaaa ctaccggcag cagtggtctt tagcgaggaa atcggcgaga tttatcagag
    25741 tttgcaaaca atgcgaagca gtttgggtaa tgcctcggca aatagctggg ataattggtt
    25801 tacaccggca gagagtgcat tgacagaggt cacagacagc tttgaccatc tagcaaaacc
    25861 ggtggcaaaa atgaccgctt ggattgtgct gcgtaccgat gaggaggcag aaaatgacac
    25921 agttgcttaa acatatcgtc aaacagggtg agcgttggga taacttgagc tatcaatact
    25981 acggcaatgc gtttgaatat ggtcgaatta ttgatgccaa tccgcatatc ggtttttgcg
    26041 aggtgttgcc gataggggta acaatttata tccctgtact taacgttaag ccaacgaata
    26101 atgaaaatat gccaccgtgg ctaagggggc gtgatgcgta atgtaccgaa gcccgatttt
    26161 tcactgtttt atgaaaaaac caatatcact gccgagattg agccacattt ggtgcaactg
    26221 acctatatcg accatttgga aggtcaatcg gacgaactca ctgtggaatt tgaagacatt
    26281 caaggcaaat ggatacgcca atggttcccg actcaggggg ataagctcaa agcggcgatt
    26341 ggctatcagg gcgaatttct gacggacatc ggtgagtttg aaattgatga agtggaatat
    26401 agttataagc cttccactat taacttaaaa gctctaagca ccggtatcag caaagccaac
    26461 cgtactttaa aaccgaaagc ctatgagaat acgactcttg ctcaggtggt ggcaaaggta
    26521 gcagataacc tgaaattaaa ggtagtcggt aagattaagg ctatcccgat taaacggatc
    26581 acccaatatc aagagcggga cgtggagttt ttggcacgcc ttgcccgaga gtatcatcac
    26641 agctttaaaa tcgtgggcaa tcagctggtc tttaccgata aaagcgaact ggggcaaagt
    26701 gagccggtgt taatgttgga agaacgggat actatttccc tacgtttgcg agatcggatt
    26761 aaagacactg caaaggcagt ggaaatcagc ggctatgata caagcggtaa aaaagtggtg
    26821 aaaaaacgca aaaaggcaaa agccttacgc ccgaatataa aacaagctaa atcagccagt
    26881 gaagatacgc tgaaaatcat cacacgaggc gaaagtcaag aacagattga tgcacggggt
    26941 gaggctgcgt tggctacgca aaatgaagat caggcggctg gtgatattga gcttatcggc
    27001 aatcctaaat tggttgccgg agcaacgatt ttattgcgta actttggcgt gtttagtggc
    27061 aagtatctga ttaaatcatc acgccatacg attagccgta gcggtggtta tactactcat
    27121 attgaggtca gaatgttgga gtttattgcc gatgatttaa tcacattagg tatggaggct
    27181 ggcaatgcaa acgcataatt ttggggcgac ttatcaagaa gggattgtat ccgctgttga
    27241 tccgaaaacg cacaaagtgc ggtgtaaaat ccctgcgctg gaagatttag aaactgcttg
    27301 gctgtcgttt ttgaccccga acgccggtgg caatcagttt tactgtttgc ccgatgtggg
    27361 cgagctggtg gcaatgctac ttgatgcaca aggcgaaggt ggctgtgtgt ttggggcaat
    27421 ttataacgag caagacccga caccggttgc cgatagcgat atgtgggtta agaaattcag
    27481 caatggcacg gagatttcgc ataatcgcaa aaccggcgat gtggtagtga agactaacgg
    27541 gcatttaacc gcaacggctc aaggtggggc tacgattaat gcaaacaccg tagtcaatgg
    27601 cacattgcac gctaccggta aaatcacctc tgacgaagaa gtctctgctc ctgttgtgaa
    27661 acaaggcacg gtagagcttg gcacacataa acacggcggt agccctgaac ctaataagta
    27721 attctttaaa ccagtttaaa atccctttta aagatagccc tgtatcatca gggctatgaa
    27781 cagacaaact tccattcaat taacccactg gcaacttgca ccctcaaccg atacagttga
    27841 ggtggtgcaa ggtattgatg atattcatct ttgtattgtc aatatccttt caacccaaaa
    27901 aggcacggat attttacgcc ctgaatttgg ctctgaccac tttcgctaca ttgactaccc
    27961 tgaagatgtg gctatcccga attttgtacg ggaaattaca accgccttgc aaaaatggga
    28021 aaaacgcatt gaggttgaag aagtattggt ggacggttca gctccgcatt ttatttttac
    28081 tgtgatgtgg cacttaaccg atgatgttta tcgtgaaatt taccgaacaa cggtataagg
    28141 ataggcaatg acagaagcga ttaaaatcat tgatgacgat gtaaaaaccg tacttgccga
    28201 agcgattgcc gattatgaaa aacgcaccgg taaaatctta caacctgcac atattgagcg
    28261 ttccattatt caaacctacg cttaccgtga gcagttatta cgcaaaggca ttaatcacgc
    28321 ctttttacaa acctttccgc agtttgcgac ggggattgcg ttggatttgt gcggggaaac
    28381 ttttggttgt tatcggctta gagataaacc tgcccgtact ttactacgct tttcagtaag
    28441 tggtgaacat acagctatag ttatccctaa aggaacgcag gtggcaatta atgacgagct
    28501 atacttttca acgctcaatg acgatgttat tactccatta attacctatg tggaaattga
    28561 tgcagagtgt aatcaggttg gtgttatcgg taatggttgg gaatcaggaa gagtcagtaa
    28621 actaaaaaca gcactggcaa ctaattataa agtaacagtt gaaaacattg atattacgag
    28681 cggtgggttg atgcaggaag aagatgaacc ttaccgtaag cgaattttag ccgcaccgga
    28741 agcctttagt aactgtggct ctatcgctgc atatgattac cacgtccgct ctgtatccca
    28801 agatattgca gatgtgaata tctctacgcc taggggtggc ttggtaagaa ttgcggtatt
    28861 gacccaaaaa gggttaccgg atacccgatt acttgatgat attaaaaaac acgttagtgc
    28921 cgaacggcta cgcccacttt gcgatatggt cgaggttatt aagcccacag aacgccgtta
    28981 tcaaattacg gcacgtttaa tattgttaga tggctaccgt gaggatttgg taaaagcaaa
    29041 agcccgagag gcattacaac tctatttgtc agacaagact aaaaaactcg gtttagacat
    29101 tgtgccgtcg gccatcattt cagctttaag agttgagggt gtttacgatg tgaatttaat
    29161 tcagccagtc aaaactatta tcaacgaaac agaatgggcg aactgcacag caatcagcgt
    29221 agaagtcgaa gaggagcgta gagatggcta gcttgcacta tgcggacatt attgtgcgag
    29281 accctaaata taaagcatta gcggatttaa gcaagcggtt aaatttgctc gatacttcgc
    29341 aaattatgac tacgctggtg gatttattgg gcgacgaatg tatcccattg ctggcagaaa
    29401 aatggtcggt aaccggctat gacggcttat tggttgcaga tagcgaaaac tcaaagcgag
    29461 ccttaattaa aagagcaatc gaactacatc aactaaaagg cacgccttgg tcaattcgtg
    29521 aagtattacg cagactaggt ttcggagagg ttgagattga cgagggactg aaaaatcggc
    29581 tgtatgaaag cacttatgtg agcggaatcc ccgaaaacga acgctgggcg cactatgcga
    29641 ttaggttgag taacccgatt accaatgagc aagctcgcaa tatccgtaag gtcttacgca
    29701 attttgctcc ggctcgttgc tctcttgcag ttttggatta taaagcagat ccaattcgat
    29761 ataacaataa agcaacctac aacggcacat ataaccacgg ttcaagttaa ggataaatta
    29821 tggcaggtgt acaagaaacc gcacagtggg aaaaccacgt ttacagaatt gaagaaaatg
    29881 acccagtcca cggaggagaa aacggtgtaa ccaataagcc aattaagcac ctagcaaacc
    29941 gcacgctcta tctgcgtaga ttgctcaccg aggcaggaca acgcattaac ccgaaaaaaa
    30001 tcacagctac cagcaaaaat agtaatgatt taactgggca tactcacgag attgaccacg
    30061 caagcctaac gcaaaaaggc attgtgcagt taaatgcgag tatccaatca actagcacaa
    30121 ctgagtctgc aaccccttca gcagttaaag cagcctatga taaagcagca acagcagaac
    30181 aaaaagcgac attggcaaat gagaaagtcg aggcgattaa agtaggcgga cgaaacctcc
    30241 tcattcaatc caacactcca cacaacggga atgcgtactc agtcagatac gcattggctg
    30301 aggctccgaa agtaggcgaa gagattgtag tcactctatg gggtgaactc ggctctcaac
    30361 gtactggaat tggcgtttac aactctcaag gtgctacgga gttggcaaaa ctggtcaaaa
    30421 ttcgtgatgg agtttatcaa ggtaaaggca tttggcgtaa gccgatgagt gagggacgag
    30481 agcgaacgcc taacgacacg cacctgaatg tgtattttta cccgaaaacc gcaacagata
    30541 ataaccgtat tgagcgaatc cagcttgagc gaggctcaat cggtacagat tggacgccaa
    30601 acgaggcaga cttaaaggca gagattagtg caatccgcac taaactaatc cctgtacggg
    30661 gtggcaatgt tgctacaacc ttaaatgcgg ctaagcggtc ggattggttt agagaaatgc
    30721 aaatccctta tcaaagtggt tgggggtatt acacccaagc atttcataac tcagggacgc
    30781 aacacaacta cagcaattta ccgctgacag gtcgttatta ttttatcgcc gaactctatc
    30841 ataactctgg ttattctcac ttgcgtatca cctatccaag tttaaaacgc acctttgaga
    30901 gtgctatcaa ccttaacaat gaggatttga ttctagattg gcgtgaggtg gtgtttttaa
    30961 aagatggatt gtatcaaggc acttttaaaa caacaggcac actagagtct gcttatcgtc
    31021 taagagcggc tcgtgatgat gaacgttatc agccatacct gcaaatggtc gatatgggct
    31081 atgaccttgc caatcccccg gtaggaaaaa acaaaattat tggcagtgtt gattattacg
    31141 ttaaaaacgg cacaagcgag tcttccaaag cgacgatgat ggcagcggca atgcctgaca
    31201 agaacgtatt ttgggaagta gcattgtgga atgcagcaca gcaacggaat gtattgttta
    31261 aggggtatag caagactaac aatttgagta ttggtaaaac caccgacaat gagcgagacc
    31321 gagtacaagt caatggcaca atccaagcca ccgcaccggc tgataacgcc aataacgacc
    31381 aagtgccgac tacggcttgg gtgcgtaaga tggcaaagat tttagctgat ggcaaggtga
    31441 gtaaagccgg tgacacaatg actgggcatt tattcatcaa gaatggggaa tactctgcgg
    31501 tacataccta taacacaagt ggttggttcg caaaatggga ggcggcccca ttatctgatt
    31561 cgcactttgc tgccattgta tatgccaacg acagagagac agttatcaat cgggtgttaa
    31621 ttcccaagaa gaatggcaca gtcgctttaa ttaatgatat tgccgatacc gttagagccg
    31681 ctgtgagtcg tagtgataaa tcccgtagta gaacttttcc tctgcaaacg gggaaaaaat
    31741 atacaaccac agggagcgtt acgatttatc cagatggccg aattgtgcag attttccact
    31801 taaaaaatat taaagcggcg tggtttgatt atgaggcaag tgcggtagga gataggcata
    31861 gagttgtcaa tatccctctt tggactgcga tgccaaataa aatttttgat gtcgatgtta
    31921 aaactgttcg cccctcaaat aatgcctcaa cttactacat agaagcggct gaatggctga
    31981 gtgcgtggca gatttacggt aatgacacga acaaatcaag tgtcaatatt aatctatctc
    32041 gattcagagg tggaaaggat gaagatatgg acttgtatgt aacagttgag ggatattaaa
    32101 tggcatattt tgtgaatgtg attgatgagc aaggcaatta tgagctaatt gatgatgagt
    32161 ttattgggct gtatccggat ttggatttta acacattacc caaactcacg gataagcaat
    32221 atcagaccta ccttgccaaa gcaaacggca aagagcgatt tgtaaatggc gaatttgtgt
    32281 atgagcaaat taaggtggat atgcaagcgg tcatttccgc tgaaaaatct gcaaagttag
    32341 cagagattaa ccaaaaagca caagccttta tcaatgattt agccaaatac aacgaaacgc
    32401 ctaagtttga gcgtgatact tggcttgagc aagcgaaaga ggcaaaagcg tgggtggctg
    32461 acccaaccgt acaaacgcca acccttgagt taattgcgca aatgcgaggc ataccgctcg
    32521 acacgctacg ccaaaaagcc tacgaaaaag cgatggcgta tcaaacggtg gctgctattg
    32581 tagcagggca acggcaagga tacgaagacc gcctagaaca agcggaaacc ttagagcaaa
    32641 tacaggcgat tgagccgatg tatcaattac cgcaaggagc aaacaatgag caaactgact
    32701 aaattaaaat cgtggagcta ccacgtctta attgcaatag atcaactctt taacgctctc
    32761 accggtggag cagcggacga aacattttct agccgttgct accgtggtgc agtgcttgcg
    32821 gaaaatccga aaaaacgttg gcgtttttgg cactcttttt tgaattgctt atttttagac
    32881 ccaaagcact gctacgccgc ttataaagcg gaggtacacc gcaagcagta tccgaaggca
    32941 tttagacttg gggggattta atgaaaaagc aaaacttaaa attagtgcgg ggcgatgatg
    33001 tgagttttag attcagcatt aaagacagca ccggacaacc tcttgcgtta aaggatttgt
    33061 ggtttgattt gcacttagtc agctatcaag gtgaggtgct gattgctctc tctactgatg
    33121 atggcagtat tgagacagca gaggctggtc agatggtgtt acattttagt caccagcaaa
    33181 ctgctcgagc tgaatggaca agtgccaatt atgacttgca actcacgcac gactatacaa
    33241 gagtctagga ggtggagatg acctctatca ttgaactaga cctgactgat gacctcatca
    33301 ttgaggtgca gttagatagc acaatcatta ccggcgatag ctttttagcc aacctgccgg
    33361 atttactcca aatttacaac caagcaaagt cagatagcag taaggagctt aacaatgact
    33421 aaacacaaaa accacgaaaa ccacccgatt gctataacat tctaccaact tggcaaagat
    33481 gtcggcgaca ttaaccgccg gctagcggtt attgagcaac gtgagcaatc agcacagcca
    33541 agccaaccac aagctgaggc ggttacaatg ccacagcaag atgctgttgc cattaataac
    33601 atcaatgccg ttttggaggc ggtcgaaaaa tacaaaactt ttgaaaaata caacactcag
    33661 tggcaagagc gttttgaaaa attcctgtct cgtaacccga atgattacga gttaagtgaa
    33721 gagcaacgcc aaaaacttta ccacacgatg tgccagcatt tgtggcaggt gaactttact
    33781 gaaatcaata gtaaacagta tgaattaccg atcccgcaac aattccaggg taagtttggc
    33841 tatattgtca aaacctcggg cggtagcaca gaaattagtt ttgagttgct atctgaaact
    33901 cacagcactt atctttatcc ggatagctat attcccgagg gcggtggcga tgtaatgctc
    33961 aagccggctt atatttattt agtgattccg gacttagtgg caatgcgtga gccggaggtg
    34021 gtgggggttt caccgctgaa actgaacatt ctgcagaaac acctgctgac acctcagaaa
    34081 ctacaggagc agaagccctt gaacacgaag gcaaactgat tgatggcgta tggcattttg
    34141 agcgagagcc ggtaaatcca ccgagagatc agaattatta tgtggcaggt aacctgttct
    34201 acaacggcat accggattga taaattgatg tttcagtaaa cctaattagg ggggaatccc
    34261 ccttttttat tattataagg aaagaaaatg aataaatctt tagtagcgtt agctgttgca
    34321 gcgatggcat cgaaagcaca aactaaa
//
LOCUS       DANLUQ010000007        32699 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.007, whole genome shotgun sequence.
ACCESSION   DANLUQ010000007 DANLUQ010000000
VERSION     DANLUQ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 32699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32699
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.007"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(34..420)
                     /gene="rplQ"
                     /locus_tag="PXB27_000292"
     CDS             complement(34..420)
                     /gene="rplQ"
                     /locus_tag="PXB27_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDL5345122.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMASALVEHEIIKTTLPKAKEL
                     RRVVEPLITLAKADSVANRRLAFARTRNVDTVAKLFNELGPRFAQRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRPEVATEAAAE"
     gene            complement(456..1445)
                     /gene="rpoA"
                     /locus_tag="PXB27_000293"
     CDS             complement(456..1445)
                     /gene="rpoA"
                     /locus_tag="PXB27_000293"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDL5345123.1"
                     /translation="MQGSVTEFLKPHLVNIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSSKEGVQEDILEVLLNLKGLAVKVQNKDDIILTL
                     NKSGIGPVTAADITHDGDVEIVNPDHVICHLTDKNASINMRIRVQRGRGYVPASARLH
                     TQNDDRPIGRLLVDARFSPVDRIAYNVEAARVEQRTDLDKLVIEMETNGTLDPEEAIR
                     RAATILAEQLAAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAETIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIAED"
     gene            complement(1476..2102)
                     /gene="rpsD"
                     /locus_tag="PXB27_000294"
     CDS             complement(1476..2102)
                     /gene="rpsD"
                     /locus_tag="PXB27_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDL5345124.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIESKCRNRLDVAPGQHG
                     ARKPRLSDYGSQLREKQKVRRIYGILERQFRNYYTEANRLKGNTGENLLVLLEGRLDN
                     VVYRMGFAATRAEARQLVSHKSIVVNGRVVNIPSFQVSVDDVVAVREKSKKQARIKAS
                     LELATQREKPTWLEVDATKMEGVFKRRPERSDLSADINEHLIVELYSK"
     gene            complement(2131..2520)
                     /gene="rpsK"
                     /locus_tag="PXB27_000295"
     CDS             complement(2131..2520)
                     /gene="rpsK"
                     /locus_tag="PXB27_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDL5345125.1"
                     /translation="MAKTPVRARKRVKKQIADGVAHIHASFNNTIVTITDRQGNALAW
                     ATAGGSGFRGSRKSTPFAAQVAAERCAEMVKEFGLKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            complement(2537..2893)
                     /gene="rpsM"
                     /locus_tag="PXB27_000296"
     CDS             complement(2537..2893)
                     /gene="rpsM"
                     /locus_tag="PXB27_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDL5345126.1"
                     /translation="MARIAGINIPDQKHTVIALTAIYGIGKTRAKAICAATGIAEDVK
                     IRELSEEQIEKLREEVGKFTVEGDLRREVTLSIKRLLDLGCYRGLRHRRSLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            complement(3031..3144)
                     /gene="rpmJ"
                     /locus_tag="PXB27_000297"
     CDS             complement(3031..3144)
                     /gene="rpmJ"
                     /locus_tag="PXB27_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDL5345127.1"
                     /translation="MKVRASVKRMCRNCKVIKREGVVRVICSDPKHKQRQG"
     gene            complement(3169..4488)
                     /gene="secY"
                     /locus_tag="PXB27_000298"
     CDS             complement(3169..4488)
                     /gene="secY"
                     /locus_tag="PXB27_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDL5345128.1"
                     /translation="MAKQPGYQGSTQKGTGELKSRLLFVLGALIVFRIGSFIPVPGID
                     ASVLSELIRQQQGTIIDMFNMFSGGALSRASIFALGIMPYISASIIIQLLTAIHPPLA
                     ELKKEGEAGRRKISKYTRYGTLLLAFIQSIGISLALPNMVHGLVPNPSILFYVTSVVS
                     LVTGTMFLMWLGEQITERGIGNGISLIIFTGIVADLPATIGQTIEQARQGELSFIVLL
                     LVAIIAFAVTYFVVFVERGQRRIVVNYASRQQGRMMAPARTSHLPLKVNMAGVIPAIF
                     ASSIILFPASITQWFGQSEGFEWLFNLSQLLQPGQPLYVVLFTMAVIFFAFFYTGMQY
                     NPRDTADNLKKSGAFVPGYRPGEQTSRYIDKVMTRLTLIGALYIAFVCLVPYIITSLW
                     KVPFQLGGTSLLIVVVVIMDFIAQVQSHLMSQQYDSVLKKANLKGLN"
     gene            complement(4491..4925)
                     /gene="rplO"
                     /locus_tag="PXB27_000299"
     CDS             complement(4491..4925)
                     /gene="rplO"
                     /locus_tag="PXB27_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDL5345129.1"
                     /translation="MRLNSLSPAEGAKHSAKRLGRGIGSGLGKTGGRGHKGQKSRTGG
                     GVRRGFEGGQMPLYRRLPKFGFTSLKSFHVAEIRLNDLAKVDGNEVTLETLKAANIIT
                     KDILSAKVILAGKIERAVVVKGLRVTKGAKAAIEAAGGSIEE"
     gene            complement(4930..5106)
                     /gene="rpmD"
                     /locus_tag="PXB27_000300"
     CDS             complement(4930..5106)
                     /gene="rpmD"
                     /locus_tag="PXB27_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDL5345130.1"
                     /translation="MAKIKVTQTRSSIARLPKHKATLKGLGLRHIRHTVELEDTPAVR
                     GMINQVSYMVKVEE"
     gene            complement(5113..5613)
                     /gene="rpsE"
                     /locus_tag="PXB27_000301"
     CDS             complement(5113..5613)
                     /gene="rpsE"
                     /locus_tag="PXB27_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDL5345131.1"
                     /translation="MSNIEKQAGELQEKLIAVNRVSKTVKGGRIMSFTALTVVGDGNG
                     RVGFGHGKAREVPAAIQKAMEKARRNMINVALNEGTLQHPVKGSHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVRNVLSKAYGSTNPINVVRATIDALENMKSPEMVAAKRGKT
                     VEEILG"
     gene            complement(5628..5981)
                     /gene="rplR"
                     /locus_tag="PXB27_000302"
     CDS             complement(5628..5981)
                     /gene="rplR"
                     /locus_tag="PXB27_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDL5345132.1"
                     /translation="MDKKVARIRRASRARHLMREQGATRLVVHRTPRHIYAQVIAPNG
                     SEVLAAASTVEKVIKEQVKYTGNKEAAAVVGKIVAERAIAKGIKAVAFDRSGFKYHGR
                     VQSLADAAREAGLQF"
     gene            complement(5996..6529)
                     /gene="rplF"
                     /locus_tag="PXB27_000303"
     CDS             complement(5996..6529)
                     /gene="rplF"
                     /locus_tag="PXB27_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDL5345133.1"
                     /translation="MSRVAKAPVNIPAGVEVKLNGQLLTVKGKNGELSREIHNAVEVK
                     QEADALTFAPREGIANADAQAGTARALVNNMVIGVTEGFTKKLQLVGVGYRAQMKGNA
                     VALSLGFSHPVEHTLPAGVTGECPSQTEIILKSADKQLIGQVAADIRAYRKPEPYKGK
                     GVRYSDEVVRTKEAKKK"
     gene            complement(6546..6938)
                     /gene="rpsH"
                     /locus_tag="PXB27_000304"
     CDS             complement(6546..6938)
                     /gene="rpsH"
                     /locus_tag="PXB27_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDL5345134.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKVAISMPSSKLKVAIANVLAEEG
                     YIESVKVVEGVKPELEITLKYFQNKPVVESIQRVSRPGLRIYKRKDELPKVMGGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            complement(6975..7280)
                     /gene="rpsN"
                     /locus_tag="PXB27_000305"
     CDS             complement(6975..7280)
                     /gene="rpsN"
                     /locus_tag="PXB27_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDL5345135.1"
                     /translation="MAKQSMIARDVKRAKLADKFYAKREELKKIISDVNASDEDRWAA
                     VLKLQTLPRDSSPIRQRNRCRQTGRPHGVLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            complement(7292..7831)
                     /gene="rplE"
                     /locus_tag="PXB27_000306"
     CDS             complement(7292..7831)
                     /gene="rplE"
                     /locus_tag="PXB27_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDL5345136.1"
                     /translation="MAKLHDYYRDTVVNELKAKFNYSSVMQVPRIEKITLNMGVGEAL
                     TDKKLLDNAVADLAAISGQKPLVTKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLNAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTSA
                     KTDEEGQALLAAFNFPFRK"
     gene            complement(7849..8160)
                     /gene="rplX"
                     /locus_tag="PXB27_000307"
     CDS             complement(7849..8160)
                     /gene="rplX"
                     /locus_tag="PXB27_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDL5345137.1"
                     /translation="MAAKIRQNDEVIVLTGKDKGKRGKVTKVLPNGKVFVEGINIITK
                     HEKPVPALGKAGGLVKKEAAIDVSNVAIFNPETNKADRVGFRFEDGKKVRFFKSNEKT
                     I"
     gene            complement(8171..8542)
                     /gene="rplN"
                     /locus_tag="PXB27_000308"
     CDS             complement(8171..8542)
                     /gene="rplN"
                     /locus_tag="PXB27_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDL5345138.1"
                     /translation="MIQEQTMLDVADNSGARSVMCIKVLGGSHRRYAAIGDIIKVTVK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGAVIRFDGNACVILNNNTEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            complement(8765..10600)
                     /gene="glmS"
                     /locus_tag="PXB27_000309"
     CDS             complement(8765..10600)
                     /gene="glmS"
                     /locus_tag="PXB27_000309"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDL5345139.1"
                     /translation="MCGIVGAVAQRDIAEILVDGLHRLEYRGYDSAGVAVLNADDKTM
                     QIVRRVGKVKALDEALDTKPLLGGTGIAHTRWATHGEPSETNAHPHRSGKIAVVHNGI
                     IENYEELKIVLQERGYVFQSQTDTEVIAHLVEWELRSASSLLEAVQKTVVQLRGAYGT
                     VVLNEEEPEHLIVARSGSPLVIGYGVGENFLASDPLALLSVTRRFAYLEEGDVAEITR
                     RTVDIYDREGNKAEREIHEGNFEADAADKGQYRHYMQKEIFEQPVAIMNTLDGRIKDG
                     KVDISAIAPNAAEILSKVEHVQIVACGTSYNAGMVSRYWFESIAGVSCDVEIASEFRY
                     RKFVTRPNSLLITLSQSGETADTLAALRLAKESGYMAAMTVCNVASSSLVRESDFAFL
                     TKAGVEIGVASTKAFTTQLTCLLLLNVAIGRLQGRMSEEQEHHIVQSLQRLPAQIESA
                     LVFDKQIEKLSEDFADKHHTLFLGRGEYYPVAMESALKLKEISYIHAEAYAAGELKHG
                     PLALIDSDMPVVVVAPENDLLEKVKSNIEEVRARGGQLYVFADHDAGFEEAEGFKTIV
                     MPKVDEVTAPIFYTVPLQLLSYHIALIKGTDVDQPRNLAKAVTVE"
     gene            complement(10648..11409)
                     /locus_tag="PXB27_000310"
     CDS             complement(10648..11409)
                     /locus_tag="PXB27_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDL5345140.1"
                     /translation="MSKRNTQQRRHLIVQMVQEKNEVSVDELAHFFETSEVTIRKDLT
                     ALEESGFLLRKYGGAVKIPSEMMEEAFIEQLSIQKKMIAQAARDCIRDHNRIIIDSGS
                     TTGALVKALNQTGLVVMTNSLNLATELTSLECEPTVLMTGGTWDSRSESFQGKVAEQV
                     LRSYDFDQLFIGADGLDLARGTTTFNELVGLSQVMAEVSREVIVLIESQKIGRKMPNI
                     ELKWQNITKIITDSQLSFETKEQIEKMGVEVVIAK"
     gene            complement(11566..11766)
                     /locus_tag="PXB27_000311"
     CDS             complement(11566..11766)
                     /locus_tag="PXB27_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDL5345141.1"
                     /translation="MKSILKVVGAMVLMVAVSGCNNMTKTQRNTAIGAAIGGVAGHAI
                     GESTGATLGGAALGGVIGSQIK"
     gene            11991..13025
                     /locus_tag="PXB27_000312"
     CDS             11991..13025
                     /locus_tag="PXB27_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5345142.1"
                     /translation="MKLKKLSLALSTLLLGSAFANSALAEGRLTVYCSATNELCEQEV
                     QAFGKKYNVKVAFVRNGSGSTLAKIEAEKNNPQADVWYGGTLDPHSQAAEMGLLEPYK
                     SPNLEQIIEKFREPAKLKGNYSSAVYMGILGYGVNLDRIKKLGIEKVPSTWEDLLDPR
                     LAGEIQIADPQSSGTAYTAIATFVQLLGEEKAFDYFRKLHKNISQYTKSGITPARNTA
                     RGETAIGIGFLHDYAIEKKNGANIEMTAPTDGTGYELGGVSILKGARNLDNAKLFVDW
                     SLSKEAQELSWQKGNAFQVLTNTTAESSPYALDPKNLNLINYDFEKYGSSEERKRLID
                     KWVNEVKLAK"
     gene            13093..15129
                     /locus_tag="PXB27_000313"
     CDS             13093..15129
                     /locus_tag="PXB27_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016534092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL5345143.1"
                     /translation="MQNFSFSRPLVWVLIGLLGFLLLPSKALDYGLFDSTSDELLAAM
                     GWASLNITWAWFLPLLVFLLPNSTACKTRAKLELVGLGVLFAFIFLSAIVLKISLGYA
                     VLPLIIAIMGLATPNLATLKVMQGDRFIIGSLLSIILLIFFFIVYPTIAIFVSMFYNG
                     SEFVPSQVLTILQQPYVVRVIGNSLSVAGTVGVLSTLFGLAFALYTTRIAKRSAFIGR
                     IFSILPIVTPPFVVGLGVTLMLGRSGYVTEFLVDYFGFANNWLYGFTGIVIAHTLALT
                     PMSFMILEGALKSIHPSVEEAAYTLRSNRYQAFFNIIFPLLKPALANSFLVVAIQSLA
                     DFSTPLVLGGSFDVIATQIYFYIAGSQLDYASASTLGAILLVFSLAIFVIQYLWIGNR
                     SYVTVSGKSYRGDVQDLPSGMKWLIICTLLFWGTFNLVLYGSIFYGSFTVNWGVDYTL
                     TLDNYIKLFGQGFSDGGFPSLIQTVIFAAAAAPITALFGLLIAYVTVRRDFKGKKTLE
                     FLTLLCFAVPGTVAGVSYILAFNDAPIYLTGTGMIIILSMVMRNMPVGMRAAIAGLGQ
                     LDKSLDEASLSLKAGSFKTMLFVVLPLLKPALLSALVTSFVRAMTTVSAIVFLVTADT
                     RVATSYILNRVEDGEYGIAIAYGSILIVVMMAIILIFDWIVGDTRIARSKAKKM"
     gene            15227..16276
                     /gene="fbpC"
                     /locus_tag="PXB27_000314"
     CDS             15227..16276
                     /gene="fbpC"
                     /locus_tag="PXB27_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric ABC transporter ATP-binding protein"
                     /protein_id="HDL5345144.1"
                     /translation="MNNDFLVLKNVTKSFGKVTVIDNLNLSIPQGKMVTLLGPSGCGK
                     TTVLRLVAGLENPTSGQIFIDGEDVTQSSIQHRDICIVFQSYALFPHMSIGDNVGYGL
                     KMQGVPKEERLQRVKEALELVDLGGFEDRFVDQISGGQQQRVALARALILKPKVLLFD
                     EPLSNLDANLRRSMREKIRELQQRLNITSLYVTHDQSEAFAVSDEVIVMNKGKIMQKA
                     PAKELYLRPNSLFLANFMGESSIFEGTIAGNQININGYQFTLANAEQFELPDGECLVG
                     IRPEAIHLAEQGNISQLCKIKNAVYMGNHWEIIAEWEGKDLLVNTKPENFYPHIKQAY
                     VHFIEQGVFLLKKEL"
     gene            complement(16507..17691)
                     /gene="tuf"
                     /locus_tag="PXB27_000315"
     CDS             complement(16507..17691)
                     /gene="tuf"
                     /locus_tag="PXB27_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDL5345145.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKHFGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTATRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALQALNGVAEWEEKILELANHLDTYIPEPERAIDKPFLLPIEDV
                     FSISGRGTVVTGRVERGIIRTGDEVEIVGIKDTAKTTVTGVEMFRKLLDEGRAGENVG
                     ALLRGTKREEIERGQVLAKPGSITPHTDFESEVYVLSKEEGGRHTPFFKGYRPQFYFR
                     TTDVTGTIELPEGVEMVMPGDNIKMTVSLIHPIAMDEGLRFAIREGGRTVGAGVVAKI
                     IK"
     gene            complement(17751..19853)
                     /gene="fusA"
                     /locus_tag="PXB27_000316"
     CDS             complement(17751..19853)
                     /gene="fusA"
                     /locus_tag="PXB27_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDL5345146.1"
                     /translation="MARTTPISLYRNIGISAHIDAGKTTTTERILFYTGVSHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMSQQYPQHRINVIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRTGANFLRVVEQIKT
                     RLGGNSVALQLPIGAEDSFKGVVDLIKMKAINWNEADQGMTFTYEDIPADMLEACEEH
                     RAMLVEAAAEASEELMEKFFSGEELTEDEIKAALRQRVLAGEVIPVCCGSAFKNKGVQ
                     AMLDAVIDYLPAPTDIPAIKGINEDETEGERHASDDEPFASLAFKIATDPFVGNLTFF
                     RVYSGVINSGDTVYNSVKQKRERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVGTG
                     DTLCAIDAPIILERMEFPEPVISVAVEPKTKADQEKMGLALGRLAQEDPSFRVHTDEE
                     SGETIISGMGELHLDIIVDRMKREFKVEANIGKPQVSYRETIRTRVNDVEGKHAKQSG
                     GRGQYGHVVIDLYPLDAEGPGYEFVNEIKGGVIPGEYIPAVDKGIQEQLKSGPLAGYP
                     VVDLGVRLHFGSYHDVDSSELAFKLAASLAFKAAFAKASPVLLEPIMKVEVETPPDYV
                     GDVIGDLSRRRAMVNGQEANEFVVKINAEVPLSEMFGYATDLRSQTQGRASYSMEPLK
                     YAEAPKNVADAIIEARRK"
     gene            complement(19955..20425)
                     /gene="rpsG"
                     /locus_tag="PXB27_000317"
     CDS             complement(19955..20425)
                     /gene="rpsG"
                     /locus_tag="PXB27_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007526570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDL5345147.1"
                     /translation="MPRRRSIEPRKILPDPKFGSELLAKFINVLMVDGKKSTAEKIVY
                     GALETLTQRSGKEALEAFEIALENVRPTVEVKSRRVGGSTYQVPVEVRPTRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAADNKGSAVKKREDVHRMAEANKAFAHFRW"
     gene            complement(20556..20930)
                     /gene="rpsL"
                     /locus_tag="PXB27_000318"
     CDS             complement(20556..20930)
                     /gene="rpsL"
                     /locus_tag="PXB27_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDL5345148.1"
                     /translation="MATINQLVRKPRVKKVVKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRIRLTNGFEVTSYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGAL
                     DCAGVKDRKQGRSKYGVKRPKA"
     gene            complement(21051..22010)
                     /gene="rfaC"
                     /locus_tag="PXB27_000319"
     CDS             complement(21051..22010)
                     /gene="rfaC"
                     /locus_tag="PXB27_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HDL5345149.1"
                     /translation="MKILIVKTSSMGDVLHTLPAITDMQNAIPDLKVDWVVEENFTEI
                     PAWHSAVNKVIPVAIRRWRKNLCQRQTWVEWQQFLKQLKSEQYDAVIDAQGLIKSAVL
                     VTRQAKGKKFGYDKNSAREGLSSLFYDETFNIPYQQHAVERIRKLCAKSLGYALPTER
                     GDYCIAQHFAKNPQKTTAYIMLIHATTRADKHWQEAYWAELIQALDKHNLAVRLPWGN
                     QAEKERAERLASVANNAKVLPKMSLTDIATQLAGAAAVVSVDTGLSHLAAALDKPNVI
                     LYGATDPKLIGAYGQNQFYLKADKMENITSSQVLEKLLPLINK"
     gene            complement(22077..23114)
                     /gene="waaF"
                     /locus_tag="PXB27_000320"
     CDS             complement(22077..23114)
                     /gene="waaF"
                     /locus_tag="PXB27_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HDL5345150.1"
                     /translation="MNILVIGPSWVGDMMMSHALYQQLKIQYPNCQIDVMAPDWCCPL
                     LARMPEVRNAISMPIGHGSFRLCERYRLGKSLRNQYDMAIVLPNSLKSAFIPLFAKIA
                     RRRGWKGESRYFFLNDLRSNKNDYPMMVQRYVALAFEKATVPTAQQLPIAYPYLTTDS
                     EQIEQTKAKFEKQLAYAENRPAIGFCPGAEFGPAKRWPHYHYAILAKMLIEQGYSVRL
                     FGSKKDEAAGEQIRLALPENLQRYCLNLAGQTDLNQAVDLIADCNAVVSNDSGLMHIA
                     AALNKPLVALYGPTSPQYTPPLSQNAEIMRLIEGGLIKIRKGEGAEGYHQSLIDIQPE
                     MVMDKLAELLV"
     gene            complement(23225..23587)
                     /locus_tag="PXB27_000321"
     CDS             complement(23225..23587)
                     /locus_tag="PXB27_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HDL5345151.1"
                     /translation="MLVACSTVVTPVEQIHQVQKEKESVKKVTKLIAQAPKVSLYRCE
                     NDKKVEVERQGRTKKALKKEIVTVSYQGTTHQLSPSVTRDGKKYTNIRWTWHETRSGK
                     AFLYNNTKKTLAANCIKQ"
     gene            complement(23705..25024)
                     /locus_tag="PXB27_000322"
     CDS             complement(23705..25024)
                     /locus_tag="PXB27_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="opacity-associated protein A"
                     /protein_id="HDL5345152.1"
                     /translation="MTQNNFRKEPVFGESSVTSQDAEATKTDTAQAPYVSLRSTKAPG
                     HTFTPIMKRPDAKVEDHTADVFSAKQAKEFQFTPSSEATEAVEPDNTIHSVEESVSTA
                     SFNAVEAQENVEKEVVKPAPESFTTERIIPNAATAAAAATAAAAATKTKEIKAKMPPK
                     TRRLLLVALLALALLVLFFLLKPSTPETVEELQSQQGGSLPIEFRPVDEAEAKRAEEQ
                     ARAEQEAAEAAKAQEQAQAAQQESVTVAPAQTEQTMAKPSQDSQSQTQSQAVVEQPVA
                     VKPVENKPVVAQPAIKPKTENSVVFQPETTVKQQPKAPAKTEVKTERATLAAKETAKT
                     AAKPVEKAPTKTEAVKAAPAAESANAVATKTMTVPKGVSLMQVFRDNNLNISDVNAMS
                     KVNNVVSNLKVGERVTVRLDKNNRVVEMSIGSGGKFTRQANGSYTFK"
     gene            25317..25655
                     /locus_tag="PXB27_000323"
     CDS             25317..25655
                     /locus_tag="PXB27_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HDL5345153.1"
                     /translation="MKKIKQLGLTFLFGLNAMAFAQTPPTTMPVEQNAVTQPVQQTQI
                     TNANAVNINTASAAELQDKLVGIGQKKAQAIVDYRTKNGNFKNIEQLAEVSGIGQATV
                     EKNRANIILE"
     gene            25820..26713
                     /gene="psd"
                     /locus_tag="PXB27_000324"
     CDS             25820..26713
                     /gene="psd"
                     /locus_tag="PXB27_000324"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251643.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HDL5345154.1"
                     /translation="MQLKKYPVPTYFQRVKIALHYLLPQLAITRAAGWLAEQKWGVVT
                     HLIIKLFAKQYKVNLSEAKKTEPSDYATFNEFFIRELKENARPINQDPNTLCLPAEGK
                     VSESGEISDNRLLQAKGHHFTLETLLANDEEMAAKFKDGTFITTYLSPTDYHRVHMPC
                     DGTLRKMIYVPGELFSVNPFLAEHVPNLFARNERVICEFDTAFGPMLQILVGATVTAS
                     MSTVWAGVINPPRADEVVVWNYETEGEKAIKLQKGEEMGAFRLGSTVINLFPKGKVHL
                     NPALIAGMQTRMGEELGRIIN"
     gene            26785..29136
                     /locus_tag="PXB27_000325"
     CDS             26785..29136
                     /locus_tag="PXB27_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL5345155.1"
                     /translation="MYTLQELKQMNEQEIRSRLISPAIRNAGWSDRQIGEEYTFKTNK
                     RFTDGQVVVDPKTTQTKRIEAKRVDYLLYTSANQKIAVVEAKDNHHSSRHGLQQAMTY
                     ARLLDVPFAYSSNGDEFVEHDFITGVQRTLPMSAFPTPDELHQRWAKQYTTEELKIIQ
                     QPYYTSSTDTFKEPRYYQEIAINRTVEAVAKGQQRILLVMATGTGKTYTAFQIIHRLK
                     TSGAKKKILFLADRNVLVDQPMMQDFKPFKDKMVKVSNKKLDSAYEVYLALYQQLDGE
                     TDNELFKQFQPSFFDLIVIDECHRGSAKEDSDWRAILKYFKDATHIGMTATPKETDDA
                     SNITYFGEPIYTYSLKQGIEDGFLAPYRVIRVDLDCDLKGWRPTKGLKDHQGNEIEDR
                     LYTVKDFDYNIVLNDRTRLVAERICEFLRKTDRLQKTIVFCANINHAERMVAELSRLN
                     ADMLKHNSKYVCQITGDQSGKGVDLEEFCDVANEGKDYPVIAVTSKLMTTGVDSKTCK
                     LVVLDANIQSMTEFKQIIGRGTRLRPDFGKEFFTILDFRGVTRLFADPAFDGEPVVIL
                     DVPENKPLVEQPLPKPSDKDIIKDPELKPYKIPVVVDFGTLKLRILDEKVQYLNEQGE
                     LVSEDIDLFSKREMQKEFGSYEQFQQQWTTSGEIFSKFYTDPKWLAALRQTRQFSHDV
                     EDFDVLSHISFGKKPLTKTERAEKVKQSGYVEQYSPENQQVLGLLLNEYVKSGNQDLT
                     KTEILKTPQFSRFGGLVSIIKNFGGKELYQKAVKGLEKMIYSE"
     gene            29184..30653
                     /locus_tag="PXB27_000326"
     CDS             29184..30653
                     /locus_tag="PXB27_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HDL5345156.1"
                     /translation="MSLNNIVKRLQDVMRNDAGINGDAQRIEQIVWILFLKIYDAKEQ
                     EWELENDEYHSILPNFLRWQNWAEDKKDGKAMTGDELLSFVNNELFPTLKNLPISADT
                     PMNQRIIRAAFEDNNNYMKNGVLLRQVINIIDEIDFAHYQERHAFGDIYENILKSLQS
                     AGNAGEFYTPRAVTDFMVQAIKPKLGERIADFACGTGGFLTSALKVLESQIQTLSDRT
                     LFNNSVYGIEKKALPHLLCITNLLLHDIDNPNVHHDNALEKSVKDYTENDKFDVILMN
                     PPYGGSEIEQIKKNFPTALQSSETADLFMSVIMYRLKQNGRAAVVLPDGFLFGTDNAK
                     VAIKKKLLEEFNLHTIIRLPHSVFAPYTSITTNILFFDKTEPTRETWVYRLDMPQGYK
                     NFSKTKPMKLEHFNEVVEWWGNRQVIEVEGFDKAKSFTLKEMSDRQYNLDLCGFPHEE
                     EEILPPDELIADYQRKRTAYNADIDKILAEITDILGIKL"
     gene            30666..32327
                     /locus_tag="PXB27_000327"
     CDS             30666..32327
                     /locus_tag="PXB27_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HDL5345157.1"
                     /translation="MLTWEHIEKMIAHQQFIREKAEIEYKEAKNSVPKDLWHTYSAFA
                     NSNGGFILLGIKENEQGHFPELLISGVNDPDKVIDELFSQSRSEKVSKSLLKDNDVKI
                     FQVENKSVIAIYVKAADILDRPIHLNGDYRLSYVRLNSGDHKLTSSELRSFISSYSQK
                     DADGRIVPYASIDDISLSTLDKYRNLLKAHNSTSKLLTLSDEKLLQEIGAYKKDLAQN
                     SEGITNAGLLMFGKHYAITSIFPHFFFEYYEEAGDVKRYTLRITDFDLEEGNLFEFYL
                     KIVPLIQELGKDKHFKLDNLTRTEENEITDGLREALINAITHADYFNNVRHLKVIKTK
                     DNKLIFENAGVMLVDIPTAIAGQRSECRNSIIHNLFHRLGLCERQGQGVRDIYSYWQS
                     RNFNSPLLESEIDYTKLTLTFQSGMIADFEEKFTACFGENFSELQPIQKDCLLFIAFN
                     QGKAMFAELAQKLPQYPRRNITLALARLKEKGLLTSSGSPRKQLYTLVNLEWIITEKS
                     QDDYFNPPFDQLDKASESKGAPPKKSKVKQVKQLPQNKANKLPPK"
     gene            32357..32509
                     /locus_tag="PXB27_000328"
     CDS             32357..32509
                     /locus_tag="PXB27_000328"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345158.1"
                     /translation="MKSKEIMKKLRLKDDKHFRMQYLKPALTLGVIEMQFPENPKHRN
                     QRYFKK"
     gene            32533..>32699
                     /locus_tag="PXB27_000329"
     CDS             32533..>32699
                     /locus_tag="PXB27_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL5345159.1"
                     /translation="MKAQQLKNAILQLAIQGKLVPQDPNDEPASELLCKIQAEKDRLI
                     AEGKIKKNKKTA"
BASE COUNT         9407 a         7125 c         6319 g         9848 t
ORIGIN      
        1 ctaccagggt tttaagctca attttgatta agattattcc gcagctgctt cagttgcaac
       61 ttctgggcga tccactaact caatgtaagc cattggagca ttatcacctg cacggaaacc
      121 acattttaaa atgcgagtgt aaccacccgc acgttgtgca aaacgtggac ctaattcatt
      181 gaataattta gcaactgtat caacgttgcg tgtacgggca aaagctaaac ggcgatttgc
      241 aacgctgtct gctttagcta aagtaattaa cggctcaact acacggcgta actctttcgc
      301 ttttggtaaa gttgtcttga tgatttcgtg ttcaacaagc gcacttgcca tattacggaa
      361 catcgcttgg cgatggctgc tgttacggtt taattgacgt ccactcttac gatggcgcat
      421 aacctattcc ttcttagaaa atctataccg aaaaactagt cttctgcaat acttgcaggt
      481 ggccagtttt caaggcgcat accgagtgat aaaccacgag aagcaagaac atccttaatc
      541 tctgtaagag attttttacc caagttcggg gttttaagaa gttcaacttc tgtacgttgt
      601 actaaatcac cgatatagtg aattgtctct gctttcaaac agttagcaga acgaactgtc
      661 agctctaggt catctactgg acgaagaaga ataggatcaa attccggttt ttcttctttc
      721 acttcaggtt gacgaacatc acgcaaatca acgaatgcag caagttgttc tgctaaaatc
      781 gttgcagcac gacggatagc ttcttcagga tctaacgttc cgttagtttc catctcaata
      841 acaagtttat ctaagtctgt acgttgttca acacgagcag cttcgacatt gtaagcaatg
      901 cgatctacag gtgaaaaacg tgcatctact aataaacgac cgatagggcg gtcatcgttt
      961 tgagtatgaa gacgtgctga tgcaggaaca taacctcgac cacgttgaac acgaatgcgc
     1021 atattaattg atgcattctt atctgttaaa tgacagatta cgtgatcagg attcacaatt
     1081 tctacatcgc catcgtgtgt gatgtcggct gcagttacag ggccaattcc agatttattt
     1141 agtgtcaaaa taatatcatc tttattttgc acttttaccg ctagaccttt aaggtttaat
     1201 aatacctcaa gaatatcttc ctgaacgcct tctttgctgc tgtactcatg cagtacacca
     1261 tcaatttcaa cttcagtaac tgcacaaccc ggcatagatg aaagtaaaat gcggcgaagt
     1321 gcgttaccta atgtatggcc aaaaccacgc tctaacggct ctaatgtcac tttagcatga
     1381 gttgaactaa cttgttcaat atttactaag tgcggcttta aaaattctgt cacagaaccc
     1441 tgcatttttt cctctctttg cggttaagct aactattatt tagagtaaag ctcaacgatc
     1501 agatgttcat taatatctgc tgatagatca gaacgctctg gacgacgttt aaatacgcct
     1561 tccattttag ttgcatcaac ttctaaccaa gttggttttt cacgttgagt tgctaattct
     1621 aatgatgctt taatacgtgc ttgttttttc gatttttcac gaacagcaac aacatcatca
     1681 acagaaactt ggaatgaagg aatgtttact acacgaccgt ttactacgat tgatttatgg
     1741 ctaacaagct gacgtgcttc tgcacgtgtt gctgcaaaac ccatacgata aactacgtta
     1801 tctaaacgac cttccaataa tactagtaag ttttcaccgg tattaccttt taagcggttc
     1861 gcttctgtat aataattacg gaattgacgc tctaagatac cataaatacg gcgaactttt
     1921 tgtttttcac gtaattgact accatagtca gataaacgtg gcttgcgagc accatgttga
     1981 ccaggcgcta catcaagtct atttctacat tttgattcaa tcgcacgaac gcctgatttt
     2041 aagaataaat cagtaccttc acgacggctt agcttgagct taggacccaa atatcttgcc
     2101 attttctttc tccaattctt ttaacgtcaa ttaaacacga cgttttttcg gtggacgaca
     2161 accgttatgt ggaatcggag tcacatcagt aatattcgtg atacggaaac ctgctgcatt
     2221 taatgcacgg attgttgact cacgacctgg acccggtcct ttaaccataa cttccaaatt
     2281 ctttaaacca aactctttaa ccatttctgc acaacgctct gcagcaactt gagcagcgaa
     2341 tggagtcgat ttacgagaac cacggaaacc tgaaccacct gcagttgccc aagctagagc
     2401 attaccttga cggtcagtaa tagtaacgat tgtgttattg aaagatgcgt ggatatgagc
     2461 tacgccatct gcaatctgtt tttttacgcg cttacgtgcg cgaactggtg ttttagccat
     2521 tatttatcta ctccgactat tttttgatcg gtttgcgtgg acctttacgg gtacgcgcat
     2581 tagtcttagt acgttgacca cgtaccggta aactacgacg atgacgtaaa ccacggtagc
     2641 aacctaagtc taaaagacgt ttgatgctta aagttacttc acgacgtaaa tccccttcga
     2701 cagtaaattt accaacttct tcacgcagtt tttcaatctg ctcttcagac aattctctga
     2761 tctttacatc ttcagcaata cccgttgcag cacagatggc ttttgcacga gttttaccga
     2821 taccgtaaat tgcagttaat gcgattacag tgtgtttttg atcaggaatg ttaatgcctg
     2881 caatacgggc cactatgcac tcctattatt taagtttatt gatatactga tcttgaaaag
     2941 cccgtttcag gatactcaaa cagtatatca ggacgaatga gctgagcagt ataactgctc
     3001 agcgggttct ttgcaagaaa tatgcgtaaa ttaaccttga cgttgtttgt gtttaggatc
     3061 gctacaaatt acacgaacca caccttcacg tttgattact ttacaattac ggcacattct
     3121 ttttactgaa gcacgaactt tcattgctta tcctttatta acggcctatt aatttagacc
     3181 ttttaagtta gctttcttca acacagaatc atattgttga gacattaaat gactctgaac
     3241 ctgtgcgatg aaatccataa taacaaccac tacaatcaat aatgatgtac cacctaattg
     3301 gaatggcacc ttccataacg acgtaatgat gtaaggaacc aaacaaacaa aagcaatata
     3361 taacgcacca attaaggtta aacgtgtcat tactttatcg atataacgag atgtttgttc
     3421 gcctggtcgg taacctggta caaacgcacc tgatttctta agattatccg ctgtatcacg
     3481 aggattatac tgcattcctg tataaaagaa agcaaagaaa atcactgcca ttgtgaataa
     3541 tacaacatac agtggttgac ctggctgtaa tagttgtgaa aggttaaata accactcaaa
     3601 tccttcgctt tgaccaaacc attgagtaat gcttgccggg aataaaataa tacttgaagc
     3661 aaaaattgcc ggtattaccc ctgccatatt cacttttaat ggtaagtgag aagttcttgc
     3721 aggcgccatc attcgacctt gttggcgact tgcatagttt acaactattc ttctttgtcc
     3781 acgttcaaca aaaacaacaa aataagttac tgcaaatgca attattgcaa ctagtagtaa
     3841 gacgataaat gataactcgc cttgacgggc ttgttctatt gtctgcccta tcgttgctgg
     3901 aagatctgca acaatacctg taaaaataat caatgagata ccgtttccga taccacgctc
     3961 agtgatttgt tcgcctagcc acattaggaa cattgttcct gtcactaagc ttactacaga
     4021 ggttacatag aataatatac ttggattagg aactaatcca tgtaccatat tcggtagggc
     4081 tagtgaaata ccaatagatt gaataaatgc taataacagt gtgccatagc gtgtatattt
     4141 actgattttc cgacgacctg cttcaccttc tttctttaat tcagctagag gtgggtgaat
     4201 cgctgtcaat aattggataa taattgacgc tgaaatataa ggcataatac ctagtgcaaa
     4261 tatagatgca cggcttaaag caccaccaga gaacatatta aacatatcaa tgatggtgcc
     4321 ttgctgttgt cgaattaatt cggataatac agaagcatca atgccaggaa caggtataaa
     4381 agaaccgata cggaaaacga ttaacgcacc taaaacaaac aatagtcttg atttaagctc
     4441 gccagtccct ttttgtgtac taccttggta ccctggttgc tttgccatct ttattcctcg
     4501 atagaaccgc cagcagcttc aatagcagct ttagcgcctt tagttacacg taagccttta
     4561 acaactactg ctctttcaat tttgcctgct aaaattactt tagcagataa aatatcttta
     4621 gtaatgatgt ttgctgcttt taatgtctct aaagttactt cgttgccatc tactttagct
     4681 aagtcgttta aacgaatttc agccacatgg aatgatttta atgaagtaaa accaaatttt
     4741 ggcaaacgac gatataaagg catttgacca ccctcgaaac cacgacgaac accgccgcct
     4801 gtacgagatt tttgaccttt atgaccgcgc ccaccagttt tacctaaacc agaaccaata
     4861 ccacgaccta gacgttttgc actgtgctta gcaccttcag ctggagaaag agaatttaaa
     4921 cgcattctat tactcctcta ctttaaccat ataagacact tgattgatca taccacgcac
     4981 tgctggtgta tcttcaagtt ctacagtatg acgaatatga cgaagaccta agccttttaa
     5041 cgttgcttta tgtttcggta aacgagcgat agagctacga gtttgagtta ctttaatttt
     5101 tgccatatcc aattacccca aaatttcttc aactgttttg ccacgttttg cagcaaccat
     5161 ttcaggtgat ttcatgttct caagtgcatc gattgttgca cgaacaacgt taattgggtt
     5221 agttgaacca tacgctttag aaagaacgtt acgaacaccc gcaacttcta atactgcacg
     5281 cattgcacca ccagcgatga tacctgtacc ttcacttgct ggttgcataa atacgcgtga
     5341 acctgtatgt gaacctttaa ctggatgttg taatgtacct tcatttaaag ctacattaat
     5401 catattgcga cgagcttttt ccatcgcttt ttggattgct gctggaactt cacgtgcttt
     5461 accgtgacca aaacctacac gaccgttacc atcgcccact actgttaaag cagtgaaact
     5521 cataatacga ccacctttta cggtttttga aacacggtta accgcgatta gcttttcctg
     5581 cagttcacca gcttgttttt cgatgtttga catctcaaat tcctctatta gaactgtaga
     5641 ccagcttcac gagcagcatc tgctaatgat tgcacacgac cgtggtattt aaaaccagaa
     5701 cggtcaaaag caaccgcttt aatacctttg gcaattgcac gctcagcaac gattttaccc
     5761 actactgctg cggcttcttt attgccggta tatttaactt gctctttaat tactttttca
     5821 acagttgaag ctgctgctag tacttctgag ccattaggtg caatcacctg cgcatagata
     5881 tggcgaggtg tgcgatgcac aactaaacgt gttgcacctt gctctctcat taaatgacgt
     5941 gcacggcttg cacgacggat acgagctact ttcttatcca tagtgttacc ttactttact
     6001 ttttcttcgc ttcttttgta cgcactactt catcagagta acgtacacct ttacctttat
     6061 aaggttcagg tttgcgatat gcacgaatat ctgctgccac ttgaccaatt aactgtttgt
     6121 cagcactttt cagaataatt tctgtttgtg atggacattc accagttacg cctgctggca
     6181 acgtgtgttc tactgggtga gagaaaccta aacttaaagc aacagcattg cctttcattt
     6241 gtgctctata accaacaccc actaattgca atttcttagt aaagccttca gtaaccccga
     6301 ttaccatatt atttactagt gcacgagcag tacctgcttg agcatctgca ttagcgatac
     6361 cttcacgtgg agcgaaagtt aatgcatctg cttcttgttt tacttcaact gcattgtgaa
     6421 tttcacgaga taactcgccg tttttacctt taactgttaa tagctgaccg ttaagtttta
     6481 cctcaacacc ggcaggaata ttaacaggtg cttttgcaac acgagacatt tttcctacct
     6541 ctctattatg ctacgtaaca gataatttca ccgccgaggc ccgcttgacg cgcagcacgg
     6601 tcggtcatta cacctttaga tgtagaaaca actgcgatac ctaaaccacc cattactttt
     6661 ggtaattcgt ctttgcgttt ataaatacgc agacccggac ggctcacacg ttggatactt
     6721 tctacaaccg gtttgttttg gaaatatttt aaagtaattt ccagttcagg tttaacacct
     6781 tcaacaactt taacgctttc gatataacct tcttcagcta aaacatttgc aatagcaact
     6841 tttaacttag atgaaggcat actgattgca actttgttcg cagcttgacc gttacgaata
     6901 cgggtcagca tatctgcgat tggatcttgc atgctcatgt atttactccc atgattccaa
     6961 attaaaagag gttattacca gcttgccttc ttaagacccg gaatttcacc gcgcatagca
     7021 gcttcacgaa ccttaatacg gcttaaacca aacttacgaa gtacgccatg tggacgacca
     7081 gtttggcggc aacgattacg ctgacggatt gggctagaat cacgaggaag tgtttgtaat
     7141 tttaacactg ctgcccaacg gtcttcatca gatgcattca catcagagat gattttcttt
     7201 aattcttcac gttttgcgta gaatttatca gctaatttag cacgttttac atcgcgtgcg
     7261 atcattgatt gttttgccac gataacctac cttatttacg gaatgggaaa ttgaaagcag
     7321 ctaataaagc ttgaccttct tcatcagttt tcgctgaggt ggtgatagta atatctaaac
     7381 cacgaacacg atctacttta tcgtagtcga tttcagggaa aatgatttgt tcacgaacac
     7441 ccatactgta attaccacga ccatcgaatg acttagcgtt taaaccgcgg aagtcacgaa
     7501 tacgtggaac agcgatagta atcaatcttt caaagaattc ccacatacgt tcaccacgta
     7561 gggttacttt gcaaccgatt ggataccctt gacggatttt aaagcctgca acagatttgc
     7621 gagctttagt tactaaaggt ttttgaccgc tgattgctgc taaatccgct actgcgttat
     7681 ctaacagttt tttgtcggtc aatgcttcac ccacacccat gttaagggta atcttttcga
     7741 ttcgtgggac ttgcatgaca gatgagtagt tgaattttgc ttttaattca ttaactactg
     7801 tatctctgta gtaatcatgc agtttcgcca tcacattact ccagttaatt aaattgtttt
     7861 ctcgttagat ttgaagaaac gcactttttt gccatcttcg aatctaaatc ctacacggtc
     7921 agctttgttg gtttcagggt tgaagatcgc aacgtttgaa acatcgattg cagcttcttt
     7981 cttcactaaa ccaccagctt tgcctaatgc aggaactggt ttttcgtgtt tagtgatgat
     8041 gttgataccc tcaacaaaaa ctttaccatt tggtaacact ttagttacct taccacgttt
     8101 gcccttatct ttaccggtaa gaacaattac ttcatcgttt tgacggattt ttgcagccat
     8161 tacattctcc ttatagtact tctggagcta aagagatgat tttcataaac ttctcagaac
     8221 gaagttcacg agtcaccggt ccaaaaatac gagtaccgat tggttgctca gtgttattgt
     8281 ttaaaattac acaagcattg ccatcgaaac gaataactgc gccatctggg cgacgaacac
     8341 ccttcttggt gcgcacaaca actgctttta acacatcacc ttttttcact ttaccgcgtg
     8401 gaattgcttc ttttacagta actttaatga tatcgccaat agcagcgtaa cgacggtgcg
     8461 atccacctag aaccttgata cacattacgc tacgagcccc tgagttatca gcaacatcca
     8521 gcatagtctg ttcttggatc atattagtgc tccgttaaaa taaaaaaaca ccctttcggg
     8581 acgaacctat ttatacaaat ataaataggg tgcaaaatat actacaaaaa ccactagtta
     8641 atcaagtgaa tttttaagct aatttaatag tttaagtaag cttatttata aattgtttca
     8701 actatcaata aactgtccta taactcatat aaaaacccca agatatcttg gggtttaact
     8761 aacattactc tacagtcacc gcttttgcaa ggttacgagg ttggtcaacg tccgttcctt
     8821 tgattaaggc aatgtgatat gaaagtagtt gtaacggcac ggtgtagaaa atcggtgcgg
     8881 ttacttcatc aactttcggc attacaatcg ttttgaagcc ttctgcttct tcaaaacctg
     8941 catcgtgatc ggcaaacaca tataactgac cgcctcttgc acgcacttct tcaatattgg
     9001 atttcacttt ctcaagcaag tcattttccg gtgctaccac cactaccggc atatcgctat
     9061 caattaatgc taacgggccg tgttttaact cgcctgcggc ataagcttct gcgtgaatat
     9121 atgaaatctc ttttaatttc aatgcagatt ccatcgcaac cgggtaatat tctccacgac
     9181 ctaagaacaa tgtgtggtgt ttatccgcaa aatcttccga taatttttcg atttgtttat
     9241 cgaacactaa cgcactttca atttgtgctg gtaagcgttg taaagattgc acgatatggt
     9301 gttcttgctc ttcactcatt ctgccttgta agcgtccaat cgccacattt aataacaata
     9361 agcaagttaa ttgagtagtg aatgctttgg ttgatgctac accaatttca acaccagctt
     9421 tggttaagaa tgcaaaatcg gattcacgca ctaatgaaga acttgccaca ttacacaccg
     9481 tcattgccgc catatagcca gattctttcg ctaaacgcag tgcagctaac gtatctgcgg
     9541 tttcacctga ttgtgaaagc gtgattaata agctgttcgg gcgagtaacg aatttacggt
     9601 aacggaattc ggaggcaatt tccacatcac agctcacgcc tgcaatggat tcaaaccaat
     9661 aacgtgaaac cataccagca ttgtaagagg ttccacaagc cacaatttgc acgtgttcta
     9721 ctttcgataa aatctctgcc gcattcggtg caatggctga aatatctact ttaccgtctt
     9781 tgatacgacc atctaaggta ttcataatcg caaccggctg ctcaaaaatc tctttttgca
     9841 tatagtgacg atattgtcct ttatctgccg cgtctgcctc aaaattgcct tcgtgaattt
     9901 cacgctctgc cttgttgcct tcacggtcat aaatatccac tgtgcgacgg gtaatttcgg
     9961 ctacatcacc ttcttctaga taagcaaaac ggcgagttac gcttaataat gcaagtgggt
    10021 ctgaagcaag gaagttctcg cctacaccgt aaccaatcac taacgggcta cctgagcgag
    10081 ccacaattaa atgctccggc tcttcttcat ttaataccac tgtgccgtaa gcaccacgca
    10141 gttgcaccac ggttttttgt accgcttcca ataatgaaga agcagaacgc aattcccatt
    10201 caactaaatg agcaatgact tcggtatcgg tttgagattg gaacacataa ccacgctctt
    10261 gtaacactat ttttaactct tcgtagtttt cgataatccc gttatgaact actgcaattt
    10321 tgccactgcg gtgtgggtga gcattagttt ctgacggctc gccgtgcgtt gcccagcgag
    10381 tgtgtgcgat cccagtgccg cctaatagtg gcttagtgtc taacgcctca tctaaggctt
    10441 tgactttacc gacacggcgt acaatttgca ttgttttatc atctgcattt aatactgcta
    10501 caccagcaga gtcatatcca cggtattcta agcgatgtaa accatcaacc aagatttctg
    10561 cgatatcacg ttgtgctact gcacctacaa ttccacacat aaatttgctt tcctatgttt
    10621 aagtttaaaa attcatttga gctactgcta ttttgcaata accacttcga cacccatttt
    10681 ttcgatttgt tctttcgttt caaaacttaa ttgactgtcg gtaataattt tggtaatatt
    10741 ttgccatttc aattcaatat tcggcatttt ccgaccgatt ttttgcgatt caattaacac
    10801 aatcacttca cggctcactt ctgccatcac ctgacttaag ccaactaatt cattaaaggt
    10861 agtcgtgcct cgagccaaat ctaaaccgtc cgcaccaata aaaagctgat cgaaatcata
    10921 tgaacgcaat acctgttctg ctactttgcc ttgaaaggac tcagaacggc tatcccaagt
    10981 gccgccggtc atcaacactg ttggttcgca ttcgagggaa gtcaattccg tcgctaaatt
    11041 cagcgaattt gtcatcacca ccaagccggt ttggttcaat gcttttacca aagctccggt
    11101 cgtgctgccg ctgtcaataa tgatgcgatt atgatcacga atacaatcac gtgcggcttg
    11161 agcaatcatt ttcttttgaa tcgaaagttg ctcaataaac gcttcttcca tcatttccga
    11221 tggaattttt accgccccac catacttacg caataaaaaa ccactttctt ccaaagcggt
    11281 taaatctttt ctaatcgtaa cttcagacgt ctcaaagaaa tgagcgagtt catctacact
    11341 cacttcattt ttctcttgta ccatttgaac aattaaatgg cggcgttgtt gcgtgttacg
    11401 ttttgacatt ataatcttca cttaaatttc gtttcaaaac ttttgcatta taaagaaagc
    11461 tcgcgaaaag aaaagcagaa aatgcaaaat ttacaaaatc tatacaaaat aaaaccgctt
    11521 gtaatattga tttacaagcg gttgatttct tgaaattttt tgcaattatt tgatttggct
    11581 accaatcacg ccaccaagtg cggcaccacc taaagttgca ccggtagatt caccaatcgc
    11641 gtgaccagcc acaccgccaa ttgctgcacc gattgcagta ttacgttgcg tttttgtcat
    11701 attgttacag ccagacacag caaccattaa taccattgca ccaacaactt tcaaaataga
    11761 tttcataata atccctcata attaatcaat ttcaaataag ccaatattct tggctatgct
    11821 ataagattta atttatttag aaaggttcaa ctactaccac ctatgaattg caaatttttt
    11881 cacaatttag gccgcttgtt gattgaactg tgagcttgct cacagaattt tatggtattt
    11941 cttgctatat tgtatagcaa tctatgcgat aactcattaa ggaaataaaa atgaaattaa
    12001 aaaaattatc tttagcactc tcgactttat tactcggttc agcctttgcc aactctgctt
    12061 tagcggaagg tcgtttaacc gtttattgta gtgccactaa tgaactttgt gagcaagaag
    12121 tgcaagcatt cggcaaaaaa tataatgtta aagtagcatt tgtgcgtaat ggttcaggca
    12181 gtacattggc gaaaattgaa gcggagaaaa acaatcctca agctgatgta tggtatggcg
    12241 gtactttaga tccacattca caagctgcag aaatgggttt actcgagccg tataaatcac
    12301 cgaatctcga acaaattatc gaaaaattcc gtgagccggc aaaattaaaa ggcaactact
    12361 cttcggctgt gtatatgggg attttaggtt atggtgtgaa tttagaccgc atcaaaaaac
    12421 tcggcattga aaaagtgcct agcacttggg aagacttgct cgacccacgc ctagccggtg
    12481 aaatccaaat tgccgaccca caaagctccg gcacggctta tactgcgatt gcgaccttcg
    12541 tacaactttt aggagaagaa aaagccttcg actatttccg caaattacac aaaaacattt
    12601 cacaatatac caaatccggc atcacaccgg cacgcaatac cgcccgtggt gaaaccgcta
    12661 tcggtatcgg tttcttacac gactatgcga ttgagaagaa aaacggggct aacattgaaa
    12721 tgaccgcacc aacagacggc acaggctacg aattaggtgg tgtgagtatc ctcaaaggtg
    12781 caagaaatct tgataatgca aaactgttcg tggattggtc gctttctaaa gaagctcaag
    12841 aactctcttg gcaaaaaggc aatgcgttcc aagtattaac caacaccacc gcagaatctt
    12901 ccccttatgc tctagatccg aaaaacttaa acttaatcaa ctatgatttc gagaaatacg
    12961 gctcgagcga agagcgtaaa cgcttgattg ataagtgggt aaatgaagta aaattagcga
    13021 aatagtgtta atctttccac tgaggggata agcaattatc ccctctctaa ttataagaat
    13081 ctgtgaggtg ttatgcagaa tttttccttt tcccgaccgc ttgtttgggt gcttatcggg
    13141 ttactcggtt ttctcttgtt accgtctaaa gccttagatt atgggctgtt tgactcaacc
    13201 tctgatgaac tgctcgccgc aatgggctgg gcgagtctga acatcacttg ggcgtggttt
    13261 ttaccgcttt tggtattttt attgccaaat tcgaccgctt gtaaaactag agccaagctg
    13321 gaattagtag ggctgggcgt gctgttcgcc tttattttcc tgtcagcgat tgtgctgaaa
    13381 atcagcctcg gttatgcggt gttgccgttg attattgcca ttatggggct tgcgacccca
    13441 aatttagcaa cgctcaaagt aatgcagggt gaccgcttta ttatcggctc gttactgagc
    13501 attattctgc tgattttctt ttttattgtg tatccgacca tcgccatttt tgtgtcgatg
    13561 ttctacaacg gctcggaatt tgtgccaagc caagtgctga caattttgca acaaccttat
    13621 gtcgttcgag tcatcggtaa ctcgctctcg gttgccggca cggtgggcgt tctctccacc
    13681 ctattcggct tagcctttgc tctctacacc actcggattg ctaaacgctc ggcgtttatc
    13741 gggcggattt tctcgatttt acctatcgtc accccgccgt ttgtggtcgg tttaggcgta
    13801 accctgatgc ttgggcgttc cggctatgtt actgaatttt tagtggatta ctttggcttt
    13861 gccaacaatt ggctgtacgg tttcaccggt attgtgatag cccataccct cgcgctcacg
    13921 ccaatgtcgt ttatgatttt ggaaggggca ttgaaatcca ttcacccatc ggtggaagaa
    13981 gcagcttaca ccctgcgttc caaccgctat caagcctttt ttaacattat tttcccattg
    14041 ttaaaaccgg cattggcaaa ctccttttta gtggtggcaa ttcaatccct cgccgatttc
    14101 agtacgccgt tagtattggg cggtagcttt gatgtgattg caacccaaat ctacttctac
    14161 attgccggtt ctcaactgga ttatgcctct gctagcacgc ttggggcaat tttgttggtg
    14221 ttctccttag ccattttcgt gatccaatac ctctggatcg gcaatcgctc ttatgtgacg
    14281 gtgtccggca aatcctatcg gggtgatgtc caagacttac caagcggtat gaaatggctg
    14341 attatttgca ccttattgtt ctggggaacc ttcaacttag tgctgtacgg cagtattttc
    14401 tacggcagtt tcaccgtgaa ctggggtgtg gattatacct taacccttga taactacatc
    14461 aagctattcg ggcaagggtt tagcgatggc ggtttccctt cgttaattca aaccgtgatt
    14521 ttcgctgccg ctgccgcccc gattactgca ctattcggct tgctgatcgc ctatgtgaca
    14581 gttcggcgtg atttcaaagg caagaaaacc ctggaatttt taaccctgct ctgctttgcc
    14641 gtaccgggaa cggtggcagg tgtttcttac attctcgcct ttaacgatgc cccgatttac
    14701 ctaaccggca ccggtatgat catcattctc tcaatggtaa tgcggaatat gcctgtgggt
    14761 atgcgagcgg caattgccgg cttagggcag ttggataaat cgctggacga agcctcgctc
    14821 tcgctgaaag ccggttcgtt caaaacgatg ctgtttgtgg tgttaccgct actcaaaccg
    14881 gcactgctct ccgccctcgt caccagcttt gtgcgggcaa tgacgactgt cagtgcgatt
    14941 gtattcttag tcaccgccga cactcgtgtg gcaacctcct acattctcaa ccgtgtggaa
    15001 gacggtgaat acggcattgc gattgcctac ggctcaattt taattgtggt gatgatggcg
    15061 attatcctga tttttgactg gattgtcggc gacacccgta tcgcccgttc taaggcgaaa
    15121 aaaatgtaaa aagacatcta ctctctccct ttatgggaga gagacagaaa aatacttcgt
    15181 tcagaagtat ttttcagaga gagggatata attaaaagga caatatatga acaacgattt
    15241 cttagtatta aaaaacgtca ccaaatcttt cggcaaggtg acggtcattg ataatctcaa
    15301 cctcagtatt ccgcaaggta aaatggtgac attactcggg ccgtccggct gtggtaaaac
    15361 caccgtactg cgtttagtgg cagggctgga aaacccgact tcggggcaaa tttttattga
    15421 tggcgaagat gttacccaaa gttcgatcca acaccgtgat atttgcattg tgttccaatc
    15481 ttatgcgtta tttccgcata tgtcgattgg cgataatgtc ggctacggct tgaaaatgca
    15541 gggcgtaccg aaagaagaac ggctgcaacg agtgaaagag gcgttggaat tggtggattt
    15601 aggcggcttt gaagatcgct ttgtcgatca aatctctggc ggacaacaac aacgtgtcgc
    15661 cctcgcccgt gccttaattc tgaaaccgaa agtgctactg ttcgatgaac cgctgagtaa
    15721 ccttgacgct aacttacgcc gttcaatgcg ggagaaaatc cgtgaattac agcaacgttt
    15781 aaacattact tccctttatg tgactcacga tcagtccgaa gcctttgcgg tttcagatga
    15841 agtaattgtg atgaacaagg gaaaaattat gcaaaaagct ccagccaaag agttatacct
    15901 tcgccctaat tctttattct tagcaaattt tatgggtgaa agcagcattt ttgaaggcac
    15961 gatagctggc aaccaaatta atataaatgg atatcaattt acgttagcaa atgctgaaca
    16021 atttgagtta cccgatgggg aatgcttagt tggcattcgt ccagaagcta ttcatttagc
    16081 tgaacaagga aatataagtc aactctgtaa aattaaaaat gcagtttata tgggaaatca
    16141 ttgggaaatt atagctgaat gggaagggaa agatttatta gtcaatacta aacctgaaaa
    16201 tttttaccct catatcaaac aggcctatgt acattttatc gaacaaggtg tttttctttt
    16261 aaagaaagag ctataacgaa tttatactaa attaagagga aggtagttat aactatttag
    16321 caattttgag aaaaataggc cgcatgcaaa atctcttgat gacaagcggt cttttctcgc
    16381 cgattttttg caaatgaaat gatatgatat ggctagctta atcggcataa aagcaaaaga
    16441 ggcatacctt tcgatatgcc tctttctagt ttttatttag cttttcaatt tatcaaacaa
    16501 tcttaattat ttgatgattt tagctacaac gcccgcacct actgtacgac caccttcacg
    16561 aatcgcaaaa cgtaaacctt cgtccatcgc gattgggtga attaggctaa cagtcatttt
    16621 gatgttgtcg cccggcatta ccatctctac accttccggt aattcgattg tacctgttac
    16681 gtccgttgta cggaagtaga actgtggacg gtaacctttg aagaatggag tgtgacgacc
    16741 accttcttct tttgataata cgtaaacttc tgattcgaag tctgtgtgtg gagtgattga
    16801 acccggtttc gctaatactt gaccacgttc gatttcttca cgtttagtac cacgtaataa
    16861 tgcacctacg ttctcacccg cacgaccttc gtctaataat ttacggaaca tctcaacacc
    16921 tgttacggtt gttttcgctg tatctttgat acctacgatt tcaacttcat caccagtgcg
    16981 gatgatacca cgctcaacac gacctgttac tactgtacca cgacctgaaa ttgagaatac
    17041 gtcttcgatt ggtaataaga atggtttatc aatcgcacgc tcaggctctg gaatgtatgt
    17101 gtctaagtgg tttgctaatt caaggatttt ctcttcccac tctgctacgc cgtttaacgc
    17161 ttgtaacgct gaaccacgta cgattggagt gtcatcacct gggaagtcgt attgtgaaag
    17221 aagttcacgc acttccatct caactaattc taataactct tcgtcatcta ccatgtcgca
    17281 tttgtttaag aatacgatga tgtatggtac acctacttgg cgacctaata agatgtgctc
    17341 acgagtttgt ggcattggac cgtcagttgc tgctactact aagatagcgc cgtccatttg
    17401 tgccgcaccg gtaatcatgt ttttaacata gtccgcgtgt cccgggcagt caacgtgtgc
    17461 gtagtgacga gttgctgtat cgtactcaac gtgtgaagtg ttgatggtga taccacgcgc
    17521 tttttcttct ggcgcgttat cgatttggtc aaatgcacga gctgcaccac cgaagtgttt
    17581 tgctaatacg gtagtgattg ctgctgttaa agttgtttta ccatggtcaa cgtggccgat
    17641 tgtacccaca ttaacgtgcg gttttgtacg ttcaaatttt tctttagaca ctttaatatc
    17701 ttcctaaaaa atgagccata gccctattgc tatggctcag gttaaacaaa ttattttcta
    17761 cgagcttcaa taattgcatc agcaacgttt ttcggtgctt cagcatattt taaaggttcc
    17821 atagagtatg atgcacgacc ttgagtttgt gaacgtaagt ctgttgcata accaaacatt
    17881 tctgaaagtg gaacttcagc attgatctta acaacgaact cgttcgcttc ttgaccgtta
    17941 accattgcac gacgacggct taagtcaccg attacatcac ccacgtaatc cggtggagtt
    18001 tcaacttcca ctttcatgat tggctcaagt aataccgggc ttgcttttgc gaacgccgct
    18061 ttaaatgcta atgatgcagc aagtttaaac gcaagttcag atgagtcaac atcgtggtat
    18121 gaaccgaagt gtaaacgcac acctaaatct actaccgggt aacctgctaa cggaccagat
    18181 ttaagctgtt cttggatacc tttatcaacc gcaggaatgt attcaccagg gattacacca
    18241 cctttgatct cgtttacaaa ctcataaccc ggaccttcag catctaatgg gtataagtcg
    18301 atcacaacgt gaccatattg accacgacca ccagattgtt ttgcgtgttt accttcaaca
    18361 tcattaacac gagtacggat agtttcacgg taagatactt gtggtttacc gatatttgct
    18421 tccactttaa actcacgttt catacggtca acgatgatgt ctaagtgtaa ctcacccata
    18481 cctgaaatga tcgtttcacc agattcttca tctgtgtgaa cacggaatga agggtcttct
    18541 tgtgctaaac gacctaaagc taagcccatt ttctcttggt cagctttggt ttttggttca
    18601 accgcaacag agattaccgg ctcagggaat tccatacgct caagaatgat tggtgcatcg
    18661 attgcacata atgtgtcacc tgtaccaaca tcttttaaac cgattgctgc agcgatatcg
    18721 cccgcacgaa cttctttgat ctcttcacgt ttgtttgcgt gcatctgtac gatacgacca
    18781 aaacgttcac gtttttgttt tactgagttg taaactgtat caccagagtt aattacacca
    18841 gagtaaacac ggaagaaggt taagttacct acgaatgggt ctgttgcaat tttgaatgct
    18901 aatgaagcaa atggctcatc gtcgcttgcg tgacgctcac cttcagtttc atcttcattg
    18961 atacctttaa ttgcaggaat atcagttggt gccggtaagt aatcgattac cgcatcaagc
    19021 attgcttgta cacctttgtt tttaaatgca gaaccacagc aaacagggat tacttcacct
    19081 gctaatacac gttgacgtaa tgctgctttg atttcatctt cagttaattc ttcaccagag
    19141 aagaattttt ccattaattc ttcagaagct tcagctgctg cttcaacaag cattgcacgg
    19201 tgttcttcac aagcttcaag catatctgcc ggaatatctt cataagtgaa ggtcatacct
    19261 tgatcagctt cgttccagtt gattgctttc attttgatta aatcaacaac acctttgaag
    19321 ctgtcttctg caccgattgg aagttgtaat gcaactgagt taccacctaa acgcgttttg
    19381 atttgctcaa caacacgtaa gaagtttgca cctgtacggt ccattttgtt tacgaacgcg
    19441 atacgtggca ctttatattt gttagcttga cgccatacag tttcagattg tggttgaaca
    19501 ccaccaactg cacaataaac catcaccgca ccatcaagta cacgcattga acgttctact
    19561 tcgatagtaa agtcaacgtg tcccggagta tcaataacgt tgatacggtg ttgtggatat
    19621 tgttgtgaca taccagacca gaatgctgtt gttgcagcag atgtgatcgt gataccacgc
    19681 tcttgttctt gttccatcca gtccattgtt gcagcaccat cgtgaacttc accgattttg
    19741 tgacttacac ctgtatagaa caagatacgc tctgtagtag ttgttttacc cgcatcgatg
    19801 tgcgcactga taccgatgtt acggtaaagt gaaatagggg ttgtacgagc catttttatt
    19861 tattaccttg tttggggttt aaattaaaat tcttaataag ggtaaggctt caccgcaaac
    19921 tgagatgaag cctttgataa gtttgcttaa acgattacca acggaagtga gcaaacgctt
    19981 tgttagcttc agccatacgg tgaacgtctt cacgtttctt cactgctgaa cctttgttgt
    20041 ctgctgcatc tgataattcg ttagctaaac gtaatgccat tgatttgtca ccacgtttac
    20101 gagcggcttc tacgatccaa cgcattgcta aagcattacg acgggttggg cgaacttcta
    20161 caggaacttg gtaagtagaa ccacccacac ggcgagattt aacctcaaca gttggacgta
    20221 cgttttctaa tgcgatttcg aaagcttcta acgcttcttt accagaacgt tgtgttaaag
    20281 tttctaatgc accgtaaacg attttttctg cagtagattt tttaccatct accattaaca
    20341 cgttaataaa ttttgcaagt aattctgaac cgaacttcgg atctggaaga attttgcgtg
    20401 gttcaatact acgacgacgt ggcattggaa atttctccgt atattaaatc ttcaggatat
    20461 ccaaaactca tctaatctgc cttaaggcat cttgactgga gtttatggtt taaatattaa
    20521 aatttagacg tttggcctta ctcaacggag aaccattaag ctttaggacg tttaacgccg
    20581 tatttagaac gaccttgttt acgatcttta acgcctgcac agtcaagtgc accacgtaca
    20641 gtatggtaac gaacacccgg taagtcttta acacgaccac cacggattaa tacaacactg
    20701 tgttcttgaa ggttgtgacc ttcaccaccg atgtatgaag ttacttcaaa gccatttgtt
    20761 aaacgaatac gacatacttt acgtaatgct gagttcggtt ttttaggtgt agtagtgtat
    20821 acacgagtac acacgccacg tttctgcggg caagcctcta atgcaggaac gttacttttt
    20881 acaacctttt tcacacgcgg tttgcgtact agctggttga tagttgccat taaaaaagct
    20941 ccagttaaaa ttattaaaaa taaaattaaa aacatccttt cacacgaaag gacggtgaat
    21001 tctaaattga acgtggataa aagtcaagca cagtaagaga acaacaatga tcatttgtta
    21061 atcaatggaa gtaatttttc tagcacttgt gaagaggtaa tattttccat tttatctgct
    21121 tttagataaa actgattttg cccgtaagcc ccgataagtt ttggatcggt tgccccatat
    21181 aaaatcacat ttggcttatc taacgcagcc gccaaatggc tcaagccggt atccaccgac
    21241 accactgccg ctgctcccgc aagttgagta gcaatatcgg ttaagctcat ttttggcaat
    21301 accttagcat tattcgccac actcgctaaa cgctccgccc gttctttttc ggcttggttt
    21361 ccccaaggca atctgactgc cagattgtgc ttatccaacg cttgaatcaa ctccgcccaa
    21421 taggcttctt gccaatgttt atccgctcgg gtagtggcgt gaatcagcat aatgtaagcg
    21481 gtcgtttttt gaggattttt tgcaaaatgt tgtgcaatgc aataatcgcc ccgttcggtt
    21541 ggcaaagcat aacctaatga ttttgcacat aattttctga tgcgttctac agcgtgttgt
    21601 tggtagggga tattaaacgt ttcatcgtaa aacagactac ttaacccttc tcgggcagag
    21661 tttttatcat agccaaattt tttgcctttg gcttggcgag ttactaacac cgcactttta
    21721 atcagccctt gagcatcaat taccgcatca tactgctcac tttttagctg ttttaaaaac
    21781 tgctgccatt ctacccaagt ctgtctttgg caaaggtttt tccgccagcg gcgaatagcc
    21841 accggaatta ctttatttac cgctgaatgc cacgcaggaa tttcggtaaa attctcctcc
    21901 accacccaat ctacttttaa atcgggaata gcattttgca tatcggttat ggctggaagc
    21961 gtatgtaata catctcccat tgatgaggtt ttaacgatta aaattttcat cttttataaa
    22021 caaacaagcg gtcttttttt gcaaaaaatt ggcaaattca accgcttgtt atccatttaa
    22081 actaacagtt cagctaattt gtccatcacc atttcaggct gaatatcaat taaactttgg
    22141 tgatagccct ctgccccttc gcctttgcgg attttaatca aaccgccttc aattaagcgc
    22201 atgatctcgg cattttggga taacggcggc gtatattgtg ggctggtcgg tccatataag
    22261 gcgaccagcg gtttatttag tgctgcggca atgtgcatta agccggagtc attgctcacg
    22321 actgcattac aatctgcaat caaatctacc gcttggttta aatcggtttg cccggctaaa
    22381 ttcaggcaat agcgttgtaa attttcaggt aatgccaagc gaatttgctc accggcggct
    22441 tcatcttttt tcgagccaaa taaccgtacc gaatagcctt gctcaatcaa catttttgcc
    22501 aatattgcat aatgataatg cggccaacgt tttgccggtc caaattcagc ccctggacaa
    22561 aagccgatgg caggacggtt ttccgcataa gccaactgtt tttcaaattt cgctttggtc
    22621 tgctcaattt gttcgctatc agttgtgagg tatggataag caatcggcaa ctgttgtgcg
    22681 gttggaacag tagccttttc aaacgccaag gctacatagc gttgcaccat cattgggtag
    22741 tcgtttttat tactgcgaag atcatttaag aaaaaatatc tactttcccc tttccaacca
    22801 cgacgtctcg caattttcgc aaacagtgga ataaaggcag actttaatga attaggcaat
    22861 acaatcgcca tatcatattg atttctcagg gattttccga gtcggtagcg ttcacacaaa
    22921 cgaaatgaac cgtgtccaat cggcattgaa atcgcattac gcacctcagg catacgggct
    22981 aaaagcggac aacaccaatc gggtgccatt acatcaattt ggcaattcgg atactgtatt
    23041 ttaagctgtt gataaagtgc gtgcgacatc atcatatcac ccacccaaga cggtcctata
    23101 actaaaatat tcatttgcaa aattctctta aaaatttatt cacaatcctt gtaaggattg
    23161 ttcacagctt cgctgccgtt ggcaaagcca acgttcaaaa agcgatactt tttgtgtccg
    23221 cttgttactg ctttatgcaa tttgccgcta acgttttttt agtgttgtta tacaaaaatg
    23281 ctttaccact acgggtttca tgccaagtcc aacggatatt agtgtatttt ttaccatctc
    23341 tcgttacaga aggtgaaagc tgatgcgttg tcccttgata actcactgtg actatttcct
    23401 tttttaacgc tttttttgtt cgtccctggc gttcaacttc aaccttctta tcattctcac
    23461 aacgatataa actcacttta ggtgcttgtg ctatcagttt agtcactttc tttactgact
    23521 ctttttcctt ttgtacttga tgaatttgct caacaggggt aaccactgta ctacaagcaa
    23581 ctaacatcgc tgagcttagc gttaagtaga taagtttgta agaggggcaa aataaattca
    23641 tcctttatct cctttttaaa taaaagagca acgtttgtag cgttgctctt tttcttcatt
    23701 ttatttattt aaaagtatag ctaccgtttg cttgacgggt aaatttgccg cctgagccaa
    23761 tgctcatttc aaccacacgg ttatttttat ctaaacgaac ggttactcgc tcgcctactt
    23821 ttaaattact caccacgtta ttgactttgc tcatcgcatt aacgtctgag atatttaaat
    23881 tgttatcacg gaacacttgc attagtgata caccttttgg cacagtcatt gttttggttg
    23941 ctaccgcatt tgccgactcg gcagccggtg ctgcttttac cgcttcagtt tttgtcggag
    24001 ctttctcaac tggtttagct gctgtttttg ccgtttcttt tgctgccagt gtagcacgtt
    24061 cggttttcac ttcagtttta gcaggggctt taggctgttg ttttaccgtt gtttcaggtt
    24121 ggaacaccac gctattttcc gttttaggct taatcgccgg ttgtgcaaca accggtttat
    24181 tttccaccgg tttcaccgca acaggttgct caactactgc ttgtgattgt gtttgcgact
    24241 ggctatcttg tgacggttta gccattgttt gctcggtttg ggctggagca actgttacag
    24301 actcttgttg agcggcttga gcttgttctt gagcttttgc agcttcagcg gcttcttgtt
    24361 ctgcacgagc ttgttcttcc gcacgtttcg cttcagcttc atcaaccggt cggaattcaa
    24421 tcggcaagct accaccttgc tgagattgta gctcttcgac agtttccggc gtacttggtt
    24481 ttaacaagaa aaacagtacc aataatgcta acgccaacaa tgccactaat aataaacggc
    24541 gggttttagg tggcatttta gctttaatct ctttggtttt agtggctgca gctgccgcag
    24601 tggctgctgc ggcagctgtt gccgcatttg gaataatacg ctcagtagtg aaactctcgg
    24661 gtgcaggttt aaccacttct ttttccacat tttcttgtgc ttcaaccgcg ttaaaagatg
    24721 cggttgatac actttcttca accgaatgaa tggtattatc cggctctact gcttctgtag
    24781 cttctgaact tggagtaaat tggaattctt ttgcctgctt agccgaaaag acatcagccg
    24841 tatgatcttc tactttagca tcaggtcttt tcataattgg ggtaaacgta tgccccggtg
    24901 ctttggttga acgcaacgaa acataagggg cttgagctgt atctgttttg gttgcttcag
    24961 cgtcttgaga ggtaaccgaa gattcaccaa aaaccggttc tttgcgaaaa ttattttgtg
    25021 tcatattaat tccaaataat agcgttgtta atgagtataa aaatggcgat attctaaagc
    25081 aattcagccg ctatttgaat ttatttaact aaatttacgc aactagcggt catttttgcc
    25141 taattttttg caaatctatt tactttcatt gaatttcaga ccaaatgcaa gtctagaagt
    25201 ttagccattt tctatttttt cgatctgtgt cgaaaaattt atttttgccg gttgagttct
    25261 attttattcc ctcttaatct tcctttcgac ctttcaacaa ctaataagga aatcctatga
    25321 agaaaatcaa acaacttggt ctaacattct tgtttggctt aaatgcaatg gcttttgccc
    25381 aaacaccgcc aactacaatg cctgttgaac aaaatgcagt cactcaaccc gtacagcaaa
    25441 cacaaatcac caacgctaat gcggtaaata tcaatactgc tagtgctgct gaattgcaag
    25501 acaagctagt gggaattggg cagaaaaaag ctcaagcgat tgtcgattac cgtactaaaa
    25561 acggcaattt taaaaacatt gagcaacttg ctgaagtctc cggaattggg caagcaacgg
    25621 tagaaaaaaa tcgagccaat attattttag aataagacaa ccaaattgtt gaagtggaat
    25681 tcctacaaag cccctatctg tttttttctt tgtagccact tcaacacctt aaagattttt
    25741 tatctatttt gcattgtatt tgctatactt cgcccccaat taaacgcaaa ttctcgtttt
    25801 taaataaaag gaaaacgcaa tgcaattaaa aaaatatcca gttccaacct atttccaacg
    25861 tgtgaaaatc gctctgcact atttactgcc acaactggca attacccgtg cggcaggttg
    25921 gttagcagag caaaaatggg gcgtagttac tcaccttatc atcaaactgt ttgcaaaaca
    25981 gtataaagtc aatttatctg aagctaaaaa aaccgagccg tcagattatg ctacttttaa
    26041 tgaatttttt atccgtgaat taaaagaaaa cgcacgcccg attaatcaag atccaaatac
    26101 actttgctta ccggcagaag gtaaagtcag cgaatcaggc gaaatttcgg ataaccgttt
    26161 attacaagca aaagggcatc attttacgct agaaacctta ttggcaaatg atgaagaaat
    26221 ggcggcaaaa tttaaagacg gcacatttat taccacctac ttatcgccaa ctgattacca
    26281 tcgtgtacat atgccgtgcg atggaacatt gcgtaaaatg atttatgtgc cgggcgaatt
    26341 gttctccgtc aatccgtttt tagccgaaca cgttccaaac ttattcgctc gcaatgagcg
    26401 tgtgatttgt gaatttgaca ccgcattcgg accaatgcta cagattttgg tcggtgcaac
    26461 ggttaccgca agtatgagta cggtttgggc tggcgtaatt aatccgcctc gcgctgatga
    26521 agtggtagtt tggaattacg aaaccgaagg cgaaaaagcc atcaaactcc aaaaaggcga
    26581 agaaatggga gctttccgtt taggctcaac cgtaattaac ctcttcccga aaggtaaagt
    26641 acatctcaac cctgcactta ttgcaggaat gcaaacccga atgggggaag agttgggtag
    26701 aattatcaat taaatcctga gttaatttta cctaaactgt ttgctataat cttccgataa
    26761 gaaactctta tcggaaggaa aaatatgtac acacttcaag aattaaagca gatgaacgag
    26821 caagaaattc gctctcgatt aatctctcct gcaattagaa atgcaggatg gtcggataga
    26881 caaattggcg aagagtacac atttaaaacc aataaacgat ttacagatgg gcaagtagtt
    26941 gttgatccaa aaacgacgca aactaaacgc atcgaagcaa aacgggttga ttatctgctc
    27001 tatacttccg caaatcaaaa aattgccgtg gtcgaggcca aagataacca tcactcttcc
    27061 cgacacggct tacagcaagc aatgacatac gctcgattgc ttgatgtccc ttttgcatat
    27121 agctcaaatg gtgatgagtt tgtggaacac gattttatca ctggcgtaca acgaaccctt
    27181 ccgatgtcag catttcctac tcccgacgaa ctccaccaac gttgggcgaa acaatatacc
    27241 accgaagagc tgaaaattat tcaacagcct tattacacca gtagcactga cacctttaaa
    27301 gagcctcgtt actaccaaga aattgcgatt aaccgtactg ttgaagctgt agcaaaaggt
    27361 cagcagagga ttttattggt aatggcaaca ggcacgggta aaacctatac ggctttccaa
    27421 attatccatc gtttaaaaac cagtggtgcg aaaaagaaaa ttttatttct tgccgatcgc
    27481 aatgtactag tcgatcagcc aatgatgcaa gacttcaaac cgtttaaaga caaaatggtg
    27541 aaagtcagca ataaaaagct cgacagtgct tatgaagtct atcttgcact ttatcaacag
    27601 cttgacggtg aaaccgataa cgaactgttt aagcagttcc aaccctcttt ctttgatctt
    27661 attgtgatcg atgaatgtca tcgtggttca gcaaaagaag attctgactg gcgtgctatt
    27721 ttgaagtatt tcaaggacgc cacccatatc ggtatgactg caacccccaa agaaaccgat
    27781 gatgccagca acattaccta ttttggcgag ccgatttata cctatagcct caaacaaggt
    27841 attgaagacg gctttcttgc cccttatcga gtgattcgtg ttgatctgga ctgtgattta
    27901 aaaggctggc gaccaaccaa aggtttaaaa gatcatcaag gtaatgagat tgaagatcgc
    27961 ctctatacag tcaaagattt cgattacaac attgtgctaa atgatcgcac ccgcttggtt
    28021 gcggagagaa tttgcgaatt tttacgcaaa accgaccgct tgcagaaaac aatagtcttc
    28081 tgtgccaaca tcaaccatgc agagcgaatg gtggcagagc tttcacgcct caatgccgat
    28141 atgttgaaac acaattccaa atatgtctgc caaattacag gcgaccaatc aggcaaaggc
    28201 gttgatttag aagaattttg tgatgtagca aatgaaggga aagattaccc tgtgattgcc
    28261 gtaacctcta aattgatgac aacaggtgta gatagcaaaa cctgtaagct ggttgtactt
    28321 gatgcaaaca ttcaatcaat gacggaattt aaacaaatta tcggacgagg cactcgtctt
    28381 cgccctgatt tcggcaaaga atttttcacc attttagatt ttcgaggcgt tactcgcctg
    28441 tttgctgatc cggcatttga tggtgagccg gtggtgattt tagatgtacc ggaaaataag
    28501 ccacttgtcg aacaaccgct tcctaaaccc agtgataagg acatcattaa agaccccgag
    28561 ctaaagccat ataaaatccc ggtagttgtc gatttcggca cactcaaatt acgcatttta
    28621 gacgagaaag tccaatatct gaatgagcag ggcgagctgg tttctgaaga tattgatctg
    28681 ttctcaaaac gggaaatgca aaaagagttc ggcagttacg aacagttcca acagcaatgg
    28741 acaacaagcg gtgaaatttt ctcgaaattt tacaccgatc caaaatggct ggcagccctt
    28801 cgccaaacac gccaattcag ccacgatgtt gaagactttg atgtattaag ccacatctcc
    28861 tttggcaaaa aaccactgac taaaaccgaa cgagcagaaa aagtgaaaca aagtggctat
    28921 gtggagcagt attcgcccga aaaccagcaa gttttagggt tgctgcttaa tgaatatgtc
    28981 aaatccggca atcaagatct gactaaaacc gaaattttaa agacaccgca attcagccgt
    29041 tttggaggtc tagtgtctat tattaaaaat ttcggcggta aagaactcta ccagaaagcg
    29101 gtaaaaggct tagaaaagat gatttattcg gaataacaag cggttatttt acaacaaaat
    29161 tttacagata aacaaggatc acaatgagcc tcaacaacat agtaaaacgc ctgcaagatg
    29221 taatgcgtaa cgatgcaggg attaacggtg atgcccaacg cattgaacag atcgtctgga
    29281 tcttattctt gaaaatttac gatgctaaag aacaagagtg ggaactggaa aacgatgaat
    29341 atcactcaat tttgccgaat tttttacgct ggcaaaattg ggcggaagat aaaaaagacg
    29401 gcaaagcgat gactggtgat gaattactct ctttcgtcaa taacgaactt ttcccgactt
    29461 taaaaaatct gcctatttca gccgatacgc cgatgaacca acggattatc cgtgctgctt
    29521 ttgaagataa taacaactat atgaaaaatg gggtgttatt acgtcaggtt atcaatatca
    29581 ttgatgaaat tgattttgcc cattaccaag aacgacacgc ctttggcgat atttatgaaa
    29641 atatcctcaa aagtttacaa agtgcaggta atgcagggga attttatacc cctcgcgctg
    29701 taaccgattt tatggtacaa gcaatcaaac cgaaacttgg cgaacgcatt gccgattttg
    29761 cctgtgggac gggtggtttt ttaacttcgg cgttaaaggt attggaaagt caaattcaaa
    29821 ccctttctga ccgcacttta ttcaataatt ctgtgtatgg cattgagaaa aaagcccttc
    29881 ctcacctgct ttgtattacc aatttattgt tgcacgatat tgataatccg aatgtacatc
    29941 acgataatgc tctagaaaaa tcggtcaaag attacaccga aaatgataaa tttgatgtga
    30001 ttttgatgaa tccaccttac ggaggttcag aaattgagca aattaaaaag aatttcccta
    30061 cggctttaca gagcagtgaa accgctgatc tgtttatgtc ggtaattatg taccgcctaa
    30121 aacagaatgg acgggctgcg gtggtgctgc ctgacggatt tttgttcggc acagataatg
    30181 ccaaagttgc gattaagaaa aagctattag aagagtttaa tctgcatacg attatccgct
    30241 taccgcatag tgtctttgcc ccttatacct cgattacgac taatattctg ttttttgata
    30301 aaaccgagcc aacccgtgaa acttgggttt atcgccttga tatgccacag ggttataaaa
    30361 atttctccaa aactaaaccg atgaaattgg aacatttcaa cgaagttgtt gaatggtggg
    30421 gaaatcgcca agtgattgaa gtcgaagggt ttgacaaagc gaaaagtttt acattaaagg
    30481 aaatgtccga ccgtcaatac aacctcgatt tatgtggctt tcctcacgaa gaagaggaaa
    30541 tccttccgcc tgatgaacta attgcagatt atcagcgaaa acgcaccgct tacaatgctg
    30601 atattgataa gattctagca gaaattaccg atattttagg cattaaactg taataggagc
    30661 ataaaatgct gacttgggaa catatcgaaa aaatgattgc acatcagcaa tttatccgag
    30721 aaaaggcaga aattgaatat aaagaggcaa aaaatagtgt gccaaaagat ctttggcata
    30781 cttattcggc ttttgcaaac agcaatggtg gctttatttt attaggaata aaggaaaatg
    30841 aacaaggaca ttttcctgaa ctgctaattt ctggtgttaa tgatcctgat aaagtcattg
    30901 atgaattatt ttcccaatct cgtagtgaaa aagtcagtaa atccttatta aaggataatg
    30961 atgtgaaaat ctttcaagtt gaaaataaaa gcgtgattgc aatttatgtg aaagcagctg
    31021 atattttaga ccgtcctatc catttaaatg gtgattatcg actttcttat gttcgtttaa
    31081 attcaggcga tcataaactc acctcctctg aactgcgttc tttcatttca agctatagcc
    31141 aaaaagatgc tgatgggaga attgtgcctt atgcttctat tgatgatatc agtttaagca
    31201 cattggataa atacagaaat ctgctgaaag cccataacag cacatcaaaa ttacttacgt
    31261 tatccgatga gaaattgctg caagaaattg gggcttataa aaaagattta gcacagaata
    31321 gcgagggcat taccaatgca ggcttattga tgtttggtaa gcactatgcc attacaagca
    31381 tatttcccca cttctttttt gaatattacg aagaagctgg tgatgtaaaa cgctatacat
    31441 tacggattac cgattttgat cttgaagaag gcaatctctt tgaattttac ttgaaaattg
    31501 tgccacttat tcaagagctt ggtaaagata aacatttcaa attagataat ttaacccgta
    31561 ctgaggaaaa tgaaattact gacgggctac gggaagctct aatcaatgca attactcacg
    31621 cagattactt taacaatgtt cgccatctaa aagtcattaa aaccaaagat aacaaactga
    31681 tttttgaaaa tgcaggagta atgttggttg atattcccac ggctattgca gggcaaagat
    31741 cggaatgccg taattctatt attcataact tattccaccg tttagggcta tgtgagcgac
    31801 aaggacaagg cgttcgggat atttattcat attggcaaag ccgaaatttc aattcaccgc
    31861 ttttagaaag tgaaattgat tacactaaac tcaccttaac ttttcaatca ggtatgattg
    31921 cagattttga ggaaaaattt accgcttgct ttggcgaaaa tttctctgag ttacagccta
    31981 ttcaaaaaga ttgcttactc tttatcgcct tcaatcaagg taaagcaatg tttgcagaat
    32041 tagcacaaaa actgccccaa tatccacgca gaaatattac tttggcacta gcacgactga
    32101 aagaaaaagg cttactaacc agttcgggga gtccgagaaa acaactctat accctagtga
    32161 atttagaatg gataatcaca gagaaatcac aagatgatta ttttaatcca cctttcgacc
    32221 aacttgataa agctagtgag agtaagggcg cccccccgaa gaaaagcaaa gtgaagcaag
    32281 tcaaacaatt accccagaac aaagcaaaca aattaccccc gaagtaaatc gcttactttc
    32341 agtgttgctt gagccgatga agagcaaaga aattatgaaa aaactcaggt taaaagatga
    32401 taagcatttc cgtatgcagt atttaaaacc tgcactaacg cttggtgtga tcgaaatgca
    32461 attcccagaa aacccaaaac accgtaatca aagatacttt aagaaataac aaccgattat
    32521 tgagaataaa aaatgaaagc ccaacagcta aaaaacgcaa tcctacaact agcaatccaa
    32581 ggcaagctcg ttccacaaga tccaaatgat gaaccggcaa gcgaactcct ttgcaaaatc
    32641 caagcagaaa aagaccgctt gattgcagaa ggaaagatca aaaaaaacaa aaaaactgc
//
LOCUS       DANLUQ010000008        32372 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.008, whole genome shotgun sequence.
ACCESSION   DANLUQ010000008 DANLUQ010000000
VERSION     DANLUQ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 32372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32372
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.008"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(57..1496)
                     /gene="pyk"
                     /locus_tag="PXB27_000330"
     CDS             complement(57..1496)
                     /gene="pyk"
                     /locus_tag="PXB27_000330"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005818583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDL5345160.1"
                     /translation="MSRKLRRTKIVCTMGPATDRGNTLEKIIAAGANMVRMNFSHGIP
                     EDHIERANKVREIAARLGRHVAILGDLQGPKIRVSTFKDGKIFLNIGDKFTLDADLPR
                     GEGHQEAVGLDYKNLPNDVVPGDILLLDDGNVQLKVLEVEGVKVHTEVTVGGPLSNNK
                     GINKLGGGLSAPALTDKDKEDIKLAAKIGVDYLAVSFPQSSADLHYARELAKEAGLEA
                     KIVAKVERAETVATEEAMDDIILGADVVMVARGDLGVEIGDAALVGVQKRLIRRARKL
                     NRVVITATQMMESMIKKPMPTRAEVMDVANAVLDGTDAVMLSGETANGDYPVETVKSM
                     AEVCLGAETMPSINISRHRMEGTFKSIEEAVAMSAMYTANHLEGVSAIISLTHSGETA
                     KLTSRISSGLPIYAMSRNQKALNLCALYRGVTPVYYNEDSRTMEGAKKAIAQLKEQGY
                     LLTGELVLLTHGDELKAGGTNTCRILRVE"
     gene            1623..2111
                     /gene="sixA"
                     /locus_tag="PXB27_000331"
     CDS             1623..2111
                     /gene="sixA"
                     /locus_tag="PXB27_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HDL5345161.1"
                     /translation="MNIWIMRHGEAGFNASSDAARCLTDNGIKNTISQGKWLGEHLAN
                     QQIQLDKILVSPYLRAQQTLENVLIGMQAVNFPQHFTNLIESWDEVTPDGNPHTVESY
                     LHFLREEGAKNILLISHLPLVFDLVQILTNNQSNVQFPTSTIAEINWVKTKAELIRVK
                     HT"
     gene            2335..3324
                     /gene="dusB"
                     /locus_tag="PXB27_000332"
     CDS             2335..3324
                     /gene="dusB"
                     /locus_tag="PXB27_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDL5345162.1"
                     /translation="MQIGQYAIKNRIFLAPMAGITDQPFRRLCSQLGAGLTFSEMMST
                     NPDVWHTEKSRLRLAHHDEIGLNAVQIAGSDPVEMAKAAKINVEYGADIIDINMGCPA
                     KKVNKNMAGSALLRNPKLVAQILDSVVNAVDVPVTLKIRTGWDQENRNCLQIAKIAEQ
                     AGIKALTIHGRTRSCLFNGEAEYESIKAVKEAISIPVIANGDIDSAEKAKFVLDYTKA
                     DAIMIGRASFGNPWIFKEINDFFETGLSSPLSIDKKYQLMFKHIEDLHQFYGEEKGYR
                     IARKHVGWYTEKLNPESNFKRTFNTLSSTKEQLYALEDFVKSIHLESQLRKVG"
     gene            3329..3625
                     /gene="fis"
                     /locus_tag="PXB27_000333"
     CDS             3329..3625
                     /gene="fis"
                     /locus_tag="PXB27_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HDL5345163.1"
                     /translation="MLEQQPTQNPLTVTMLNAQAQQVNKPLRDNVKAALKNYLSQLNG
                     EDPTELYELVLSEIEHPMLDMVMQYTRGNQTRAATMLGINRGTLRKKLKKYGMG"
     gene            complement(3730..6516)
                     /locus_tag="PXB27_000334"
     CDS             complement(3730..6516)
                     /locus_tag="PXB27_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin family TonB-dependent
                     receptor"
                     /protein_id="HDL5345164.1"
                     /translation="MKYHHFRYSPVALTVLFALSHSYGAATENKKIEENNDLAVLDEV
                     IVTESHYAHERQNEVTGLGKVVKNYHEMSKNQILGIRDLTRYDPGISVVEQGRGASSG
                     YAIRGVDKNRVSLLVDGLPQAQHYHTLGSDANGGAINEIEYENIRSIELSKGASSAEY
                     GSGALGGAIGFRTKDAQDIIKEGQHWGLDSKTSYASKNSHFLQSIAAAGEAGGFEALV
                     IATHRHGKETKIHSEANKLKHNIRRITGFENRYDLYPNSAQNAPGGSFFIVEDTCPTL
                     DCTPRARVKLNRDNFPVRTFPEYTPEERKQLEQIPYRTEQLSAQEYTGKDRIAPNPLD
                     YKSNSVFMKFGYHFNSSHYLGAILEDTKQRYDIRDMQTPAYYTKDDINLSLRNYVYEG
                     DNILDGLVFKPRIPYGLRYSHVKFFDERHHKRRLGFTYKYKPENNRWLDSIKLSADKQ
                     DIELYSRLHRLHCSDYPVVDKNCRPTLDKSWSMYRTERNNYQEKHRVIHLEFDKALNA
                     GQGVFKQTHKLNLGLGFDRFNSLMDHGDMTAQYTKGGYTSYRGRGRLDNPYIYRRDPR
                     SIETVSLCNNTRGDILNCEPRKIKGDSHFVSFRDLVISEYVDLGLGVRFDQHRFKSDD
                     PWTLSRTYRNWSWNGGITLKPTEFVSLSYRISNGFRVPAFYELYGKRNHIGLKDNEYV
                     QRAQRSHQLEPEKSTNHEIGVSFKGQFGYLDVSYFRNNYKNMIATACKRIIQKSHCFY
                     NYHNIQDVALNGINLVAKFDLHGILSMLPDGFYSSVAYNRVKVKERKLTDSRLDSVND
                     PILDAIQPARYVLGFGYDHPEEKWGIGITTTYSKAKNADEVAGTRHHGIHRVDLGGKL
                     TGSWYTHDITGYINYKNYTLRGGIYNVTNRKYSTWESVRQSGVNAVNQDRGSNYTRFA
                     APGRNFSLAFEMKF"
     gene            complement(6610..8367)
                     /locus_tag="PXB27_000335"
     CDS             complement(6610..8367)
                     /locus_tag="PXB27_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HDL5345165.1"
                     /translation="MFKLKSSFVLLNAALLAACSSNGGSFDVQSAKVESQTQTTPKKP
                     SLQDDNSNARRTVSASETEALLQPGFGFSAKIPRRNLFPQGKEDVAPIGDIKEITGDL
                     TKIPYEEEVKAYGSSADGFSHTHDRNHKLYTRDFNFVRSGYVLHSGAKPEIKPKEILR
                     TGAHGYVYYLGIEPAKAIPTQKATYKGYWDFTTDARKGRDSKYFSNSAGINIGATPEN
                     SYDINVDDSEKPMGHTGEFTADFANKTLTGTLVRNGYVSRSKEQKITTIYDIDAKIKG
                     NRFSGKANAKNTDDPYFGKSSTTLEGGFFGGKAQELAGKFLADDKSVFVVFAGKRDAK
                     KDDSESAFDAFKIKLKDLNKSEMDTFGNATHLIINNKQIPLIAEGKKSFAEMKFDDLV
                     TRTIDGKTYRVSVCCNNLDYVKFGTYSEGNNSDTDTAHQYLVGERTAVADLPTGTVKY
                     RGTWDGVMYSKSGSAGAESPSNSESGTRSLFDVDFVNKKINGKLIANDGVEERPMLTL
                     EGNLKGNGFAGTAKTGNSGFNLDPKSTNGGTVVHINTQFEGGFYGPKATELGGIVHSA
                     ETDKDRVSITFGGKRQIEK"
     gene            complement(8788..9435)
                     /gene="rnt"
                     /locus_tag="PXB27_000336"
     CDS             complement(8788..9435)
                     /gene="rnt"
                     /locus_tag="PXB27_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010130043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HDL5345166.1"
                     /translation="MEQTTEQIDYKLLKHRFRGYLPVVIDVETAGLNAQTDALLELAA
                     ITLKMDEQGYLVPDQQCHFHIAPFEGANINPESLKFNGIDIDNPLRGAVAENIAIPEM
                     FKMVRKAMKEQGCQRAVIVAHNAAFDQGFLQAAIKRINAKRDPFHPFAMFDTATLAGF
                     MYGQTVLVKACQVAKIPFDGKQAHSALYDTERTAELFCAMVNRLKDLGGFPPLSD"
     gene            complement(9520..9927)
                     /gene="gloA"
                     /locus_tag="PXB27_000337"
     CDS             complement(9520..9927)
                     /gene="gloA"
                     /locus_tag="PXB27_000337"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HDL5345167.1"
                     /translation="MRILHTMLRVGDLERSIKFYTDVLGMRLLRRSENEQYKYSLAFL
                     GYADESESAVIELTYNWGVDKYELGTAYGHIALGVDDIYKTIEDVRAAGGKITREPGP
                     VLGGTTVIAFAEDPDGYKIEFIENKDAKAALGN"
     gene            10017..10832
                     /locus_tag="PXB27_000338"
     CDS             10017..10832
                     /locus_tag="PXB27_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345168.1"
                     /translation="MAHDHDSPSKLEISTHAALPMVLMLFIEMALNFYSAPKNSIFIS
                     PYLLAFFVTSLISFLVLWKGEICRGQASRLSFILPFLLIFAVGNFLYSIGFTPKHNPM
                     AVASIAAVFACLIYFRLPEDQTLYNTLMYCGLAISSIGIIQYLAIYWFELPSLFNGIR
                     ANNFAQVLLGILLAGWYLMLAKSRLDVFLKLLVKTALVVLILNYCWTAFVLYQHLQIM
                     PDMPLLSFIIYFAAQFVILLMLAWLLLGKNIKNPTAWTVATLLAILYPFANVV"
     gene            10836..11582
                     /locus_tag="PXB27_000339"
     CDS             10836..11582
                     /locus_tag="PXB27_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345169.1"
                     /translation="MKRALKFFGIATVLSVVAIYYFLMPSHYLATPINWILPKAWKVQ
                     LPQGLNLNWQGSSLAQFQLSYRDCPLLSADKTDLGWIEKNHLSVDKLDIDYRCLALFP
                     QNEHSNSDTSLNALLAVIPDGEATIHALNWKNLPTDFNPRLQRLFEQTAIKLSLSKGE
                     IQATAAQQAVKFAANFANGRLNADLSYQPNEKEQHNLRISAEIEDNFTQLPPTISAEY
                     DWQLSYEIIANKALQKGALFSVGKKMPHKS"
     gene            11768..13543
                     /locus_tag="PXB27_000340"
     CDS             11768..13543
                     /locus_tag="PXB27_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH domain-containing protein"
                     /protein_id="HDL5345170.1"
                     /translation="MPLKTAIRISLLSQNSWGKGNIVISNSDGEITEKGLNLPLRLTG
                     NIKHGNFILYSSVPLDFQGNFDDLSVKFLPSSLLRLTGKERYLTIEDLRFPLAGIKID
                     KHGIHGRLHAILRGQSPDFNNIEFHLDGQAQNFKAGALDFFQDPKDANAVKDSWKWRI
                     WGASILKAFNSKVNLSGRGQWHKQLVRLEELKGDLATVKLADTTISKITLNNTQAIRF
                     NVKKFTLDGAVMLQSPEIAFSYGGVLPKPRVNLAFDGEVEKLRFKGAVNTTELGPLKL
                     FARRQLSQDASQIIGKLYWPEQSAQGFQPLFPFRSQWVINNGTIRGETAFSAGSKNGL
                     IAGGHLAIRNGGLSLPNGEAKGIEFSLPYRYQNGRFHFGAKKPLDVKIAQIKLGDLAL
                     DNASMKLNGYYPYTKAKPLNLNQLSFDLFGGKLNVKRFALPQTELAYLNLERIDFEQI
                     LQFMQYQQIDLKGKANAILPFWLSGKPCYICDGLLTQAETSYLTFTPELLSEMQKSGY
                     TEQILLHLVDKSTINDFRSLINVGPKGDLVLDGKLKLSSNQNKSKVNLNYNHRENLFD
                     LWHLINYGSQFEQKLENYLYQKLER"
     gene            13561..13755
                     /locus_tag="PXB27_000341"
     CDS             13561..13755
                     /locus_tag="PXB27_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HDL5345171.1"
                     /translation="MKPATFYFAIFLLNLTACTPKVQLETPSEGITINMNVVVDHRID
                     VKMDEKSRAVITKSENKTKN"
     gene            complement(13752..14225)
                     /gene="folK"
                     /locus_tag="PXB27_000342"
     CDS             complement(13752..14225)
                     /gene="folK"
                     /locus_tag="PXB27_000342"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDL5345172.1"
                     /translation="MQTVYIALGSNLENPLEQLKQAVEKLKKFANEFEISPFYGSKPV
                     GPQDQPDYVNAVAKFSTDLSPTALLDKLQSIENEQGRVRVRRWGERTLDLDIILYGNQ
                     QIQTERLNVPHIEMKNREFVIVPMFDLTPDLVLPTGEKLADIYAQFKDHQMIIFS"
     gene            complement(14428..15759)
                     /gene="pcnB"
                     /locus_tag="PXB27_000343"
     CDS             complement(14428..15759)
                     /gene="pcnB"
                     /locus_tag="PXB27_000343"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HDL5345173.1"
                     /translation="MLHKKFAVNASQYRISPKDFPKNAISIVDKLQIAGYEAYIVGGC
                     IRDLLLEKKPKDFDIATNARPEEIQRIFGRQCRLIGRRFRLAHIVYGREIFEVATFRA
                     GHESHRNDKISKVSDEGMLLRDNVYGTLREDAERRDFTVNALYYDIKRNLIFDFFNGI
                     EDLRAGKLSLIGEPTIRYQEDPVRMLRAVRFMAKLDMFLDKATASPIRDMAVLLTNIP
                     AARLFDESLKLLQTGAGLKTYQLLREYELWQFLFPVLPAFFTAKGDSYAERMITKALN
                     STDERIADKLPVNPAFLFAALLWYPLREKMEQLKNEGGFNSYDAMMLAANDVLAESCN
                     AIALHRRHTSVIRDIWALQFQMMKRSGNRPQQTLEHVKFRAGFDLLVMRAEIEGGDLV
                     ELSAWWHEYQFSNEGQRTELLKSVRHLGNVPSEKKKTRRRRTFKKKPKVNG"
     gene            complement(16004..16450)
                     /gene="dksA"
                     /locus_tag="PXB27_000344"
     CDS             complement(16004..16450)
                     /gene="dksA"
                     /locus_tag="PXB27_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HDL5345174.1"
                     /translation="MTQAAKTSLGLLALAGVEPYELKKDEEYMNEAQREHFTKILKAW
                     HSQIMEEAERTKSQLQEDMTNFADPNDRASQEEEFSLELRNRDRERKLLKKIEQTLYK
                     IQEDEYGYCETCGVEIGIKRLEARPTADLCIDCKTLAEIREKQLGL"
     gene            complement(16700..17653)
                     /gene="thiB"
                     /locus_tag="PXB27_000345"
     CDS             complement(16700..17653)
                     /gene="thiB"
                     /locus_tag="PXB27_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HDL5345175.1"
                     /translation="MKFILSSLALLSASVYANQPTLTVYTYSSFASEYGPAPKLEQNF
                     EKECGCDVKFVPLDDSVTMFNRLRLEGAKTKADVVVGIDNFLQPEAEKSGLFAEFEAK
                     TKLETQQKIFFPYDTGSYAFIYDKEKLKNPPKSLKELVERQDLKVIYQDPRTSSVGRG
                     FLMWVNQVYGDEAPTAWKTLAKHTVTVGKGWSETYGAFLKGESDLVLSYTTSPLYHKW
                     HENTDKYVAAPFEEGHLVQVENAAIVKSSQQKALANQFLEFLHKPESQHIISYHNVMN
                     PVLTDGVDPAFAAMPTYKTIEFTPPTTETIQKWLADWSKSW"
     regulatory      complement(17697..17840)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            18124..19110
                     /locus_tag="PXB27_000346"
     CDS             18124..19110
                     /locus_tag="PXB27_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDL5345176.1"
                     /translation="MSQLIRTLKGHIRDEIIKKGGWVNAHAHADRAFTMTPEKITVYQ
                     NANLQQKWDLVDEVKRNSSVEDYYRRFSQAIELMISQGVTAFGTFVDIDQVCEDRAII
                     AAHKAREVYKNDIILKFANQTLKGVIEPNAKKWFDIGSEMVDMIGGLPYRDELDYGKG
                     LEAMDILMDKAKSLGKMLHVHVDQFNSPKEKETEQLCDKSIEHGMQGRVVAIHGISIG
                     SHPKEYRKMLYQKMRDSQMMVIACPMAWIDSGRKEDLQPFHNALTPADELIPEGITVA
                     IGTDNICDYMVPLCEGDMWQELSLLSAGCRFTNLEEMANIASINGRKVLGLV"
     gene            complement(19164..19583)
                     /locus_tag="PXB27_000347"
     CDS             complement(19164..19583)
                     /locus_tag="PXB27_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HDL5345177.1"
                     /translation="MLGNILGSLASSVLSGNNQSTATQLIQNLLASQGGIEGLIAKFQ
                     QGGLDDVLRTWISSDEDNAPVSGEQISNVFGQENIQNVAQEAGVDAADASDLLAQFLP
                     KIVDTLTPNGNVEDLKNFDANDLLAQAAKGILGNLFK"
     gene            complement(19661..20416)
                     /gene="kdsB"
                     /locus_tag="PXB27_000348"
     CDS             complement(19661..20416)
                     /gene="kdsB"
                     /locus_tag="PXB27_000348"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HDL5345178.1"
                     /translation="MKFTVIIPARYASSRLPRKPLLDIAGKPMIQHVWEKAQLAGASR
                     VIIATDHPEIEALAQSFGAEVCMTSEKHNSGTERLAEVIEKMQIADSEIIVNIQGDEP
                     LIPPVIVTQVAQNLDAYQVNMATLGVKLATREELFNPNAVKVVADKNGMALYFSRASI
                     PFARDSFPDCDDSFVAAQGYLRHIGIYAYRAGFVKQYVAWQPTQLEQLESLEQLRALW
                     HGEKIHIEPAKEAPQVGVDTPEDLERVRAILAR"
     gene            complement(20426..21379)
                     /gene="trxB"
                     /locus_tag="PXB27_000349"
     CDS             complement(20426..21379)
                     /gene="trxB"
                     /locus_tag="PXB27_000349"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDL5345179.1"
                     /translation="MTTKHTKLLILGSGPAGYTAAVYAARANLNPVLVTGMQQGGQLT
                     TTDEIENWPGEFEQITGTELMNKMLKHAEKFETEIVFDHIHSVDLSKRPFTLNGDLYT
                     FTCDALIIATGASAKYLGLPSEEHFKGKGVSACATCDGFFYRNKPVAVVGGGNTAVEE
                     ALYLANLASEVHLIHRRDSFRAEKILLGRLQKRIEEGKIILHTDRTVDEVLGDNMGVT
                     GLRLKSTKEELSEEIKVDGFFVAIGHAPNTAMFEGQLELENGYIKVKSGLEGNATATS
                     VEGVFAAGDVMDHNYRQAITSAGTGCMAALDAERFLDAQEA"
     gene            21546..22616
                     /gene="dhaK"
                     /locus_tag="PXB27_000350"
     CDS             21546..22616
                     /gene="dhaK"
                     /locus_tag="PXB27_000350"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012073177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDL5345180.1"
                     /translation="MKKLINSIETVLDEQLSGFVKAHSDLTLNTEPVYIYRTTPSNQP
                     KVALISGGGSGHEPMHAGFIGKGMLDGACPGAIFTSPTPDQMFECGLAVDRGEGVLLI
                     IKNYTGDVLNFETATELLADSGVKVATVLVDDDVAVKDSLYTAGRRGVANTVLIEKLL
                     GAAADKGYNLSELAKLGHQLNNNGHSIGIALSACTVPAAGKPSFTLAENEMEFGVGIH
                     GEPGIERRPFENLDSTVKQMFETLIAHGDYQRKIRHWNTQTQQWDEIEESKVALKKGD
                     RVIALVNNLGAVPLSELYAVYDALEKECQTFGLSIERSLVGSYCTSLDMQGISITLLK
                     VDEDILKLWDAPVNTPAMRWGE"
     gene            22637..23263
                     /gene="dhaL"
                     /locus_tag="PXB27_000351"
     CDS             22637..23263
                     /gene="dhaL"
                     /locus_tag="PXB27_000351"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HDL5345181.1"
                     /translation="MTISKQQILKWLENCQQVMTEKRDYLTELDTVIGDGDHGLNMQR
                     GFSKALEKAHTVQDKDIGTLLKTVGMTLLSQVGGASGPLYGSFFIKAAQGATGKEQLS
                     FNELLALLQQGAEAIVQRGHAQVGDKTMCDLWLPLLEDLAQADSTQPIAQILLQAVEK
                     AENFANQTAPLMAKKGRASYLGERSIGHKDPGATSSYYIFKALAEALQ"
     gene            23273..23680
                     /gene="dhaM"
                     /locus_tag="PXB27_000352"
     CDS             23273..23680
                     /gene="dhaM"
                     /locus_tag="PXB27_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HDL5345182.1"
                     /translation="MVNLVIVSHSAKLAEGVGEILAQMAQGGCKIALAGGIEDTSNPI
                     GTDAVKIMQAIESVFSPDGVIVFVDLGSAILSTQTAIDLLEPELAEKVIISYAPLVEG
                     AFAAVVAASAGDSLEAVLNEANEAAALKQEQAN"
     gene            complement(23720..24565)
                     /locus_tag="PXB27_000353"
     CDS             complement(23720..24565)
                     /locus_tag="PXB27_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone YbbN"
                     /protein_id="HDL5345183.1"
                     /translation="MDYFVNVNEQNFSEVWAAAAQVPTVFHFISPREVASLEMDALLR
                     RISDENPNQFLLATVNCDEQQALAAQFRIQQIPTLYLFAGGQPIDAIQGAVSEQELRI
                     RLANILPKEEELKFQQALELLQDQRYTEALPLLKSAWEMTDKKNSDFALLYAETYIAM
                     KSVEEAREILKAIPIQDRDSRWHGLQAQIELLEQASESPELQQLQADYANSKTPEIAI
                     KLANQLHQAHKNEEALTLLFDWLKSDLNVANGEVKSQFLAILSALGTDPIVPKFRRQL
                     YSLLY"
     gene            complement(24609..24935)
                     /gene="glpE"
                     /locus_tag="PXB27_000354"
     CDS             complement(24609..24935)
                     /gene="glpE"
                     /locus_tag="PXB27_000354"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HDL5345184.1"
                     /translation="METTFKEISPQQAWELIENEGAVLADVRDARRYVYSHPQDAFHL
                     TNQSYGKFLDEVDYDEPVVVICYHGVSSQSTAQFLIEQGFENVYSVKGGFEAWERNGL
                     PIETSY"
     gene            25086..25544
                     /gene="exbB"
                     /locus_tag="PXB27_000355"
     CDS             25086..25544
                     /gene="exbB"
                     /locus_tag="PXB27_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HDL5345185.1"
                     /translation="MEQLFQFLEHHTDFVILGLLGLLSVICLWKAIERIMFYSKVDIS
                     KYDTLEELDIDLTHNLTTISTIGSNAPYIGLLGTVVGILLTFYHLGQSGGDIDAASIM
                     VNLSLALKATAVGILVAIIAMVFYNGFGRKVEENKLKWLALQSKKPHQAV"
     gene            25581..26018
                     /gene="exbD"
                     /locus_tag="PXB27_000356"
     CDS             25581..26018
                     /gene="exbD"
                     /locus_tag="PXB27_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HDL5345186.1"
                     /translation="MKKFDEINIIPFIDIMLVLLAIVLVTASFISQGKIQVNVPKAST
                     TQPMKADDLAKLLTITENNEFFFNDQAITKEQLIAEVATWDKSQKVSLKVDGAVAFEK
                     FVELTDILSANEIKNVAIITKKETAPAPSSTPGSPAQVPAVAP"
     gene            26034..26945
                     /locus_tag="PXB27_000357"
     CDS             26034..26945
                     /locus_tag="PXB27_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HDL5345187.1"
                     /translation="MKKKHSRIGLLASLAIHTALIGGTFAWLHNHKVEKIAEQNSLSM
                     EMVAALLEQPQVAVAPDPITEEVKEIEPEKAEVPPEPKLTPEPTPEPEAIPDPTLNPK
                     KEKPKEEKRKERHHHKHKHRHKEKREELQEQEKPKDKPKERPKHHHKHIKALERGPEI
                     KQGIVAKAIPNAAEGEKVVAGVVGGQKNGSPNSTSTTGSANTGASSGNASELAAYKVA
                     LQRALQRKANNSYPQREKMMRKTGTVTIKFTITSSGSITNVSVLNSSGNSNLDNAAVR
                     SAEGLSMPPPPAGFPPTVTVPVRFALE"
     gene            complement(27014..27895)
                     /gene="cysZ"
                     /locus_tag="PXB27_000358"
     CDS             complement(27014..27895)
                     /gene="cysZ"
                     /locus_tag="PXB27_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HDL5345188.1"
                     /translation="MSSVFSEPQNQVGLGFHYFIYGWRLLLQRQFLPFVIFPILINTV
                     LMVGLIWGFFANIGGLLDMLLPSWLEWLSFVLIPLIFLMILVGFYFAFTTLANFVAAP
                     FNALLAEKVELQLTGEPLGDMSFGAMLKDVPRMLKREWQKMMYSIPRLIALFVLGFLP
                     VVGQTVAPVLTFIFGAWLIAIQYCDYPFDNHKISFQRMRNALSQSRTLNFTFGALVSL
                     FTMIPFVNLVVMPVAVCGATALWVKEYRNFFLNNQTGEFSKADYTFTKVNSTSLSTET
                     RTGEVSPNVRNGDIRKQ"
     gene            28007..28909
                     /gene="zipA"
                     /locus_tag="PXB27_000359"
     CDS             28007..28909
                     /gene="zipA"
                     /locus_tag="PXB27_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HDL5345189.1"
                     /translation="MELHIIFFILAGLLIAVLIGFSLWSARREKSNIFSNTFSTRPIA
                     VPPQNNKMSNSIPTSLQPQNEDYQAMSVPEENPQQIQQEVESSLQNIKISLPGGNPQQ
                     QPEQIQPIQSVAEPTVSTYGQKISNQQHIQYNTPDFANPMVNTVVEETPQAVAIEPQE
                     TQSAEEENPFVTLYLYAPQNEAFRGDFVVHYLEEVGLRFGEHQIFHRHQHIDDSLSPI
                     IFSAANLMNPGTFDLNNISNFATAGLVLFMQLPTEGNDVANMKLLISNAEHLAASLGG
                     FLYDDNQQPFGEESRQRYMQRVMH"
     gene            28974..31031
                     /gene="ligA"
                     /locus_tag="PXB27_000360"
     CDS             28974..31031
                     /gene="ligA"
                     /locus_tag="PXB27_000360"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDL5345190.1"
                     /translation="MSLFNQTSLFEENATPELVEKIETIRQTLRQYEYEYHVLDNPSV
                     PDAEYDRLMNELKNLEWQHPELISADSPTQRVGAKPLAGFAQVTHEIPMLSLDNAFSD
                     EDLDGFLRRMESYITASPSQLEFCCEPKLDGLAVSILYENGILTRAATRGDGTTGEEI
                     TANIRTIRNIPLKLNTTNPPARLEVRGEVFMPQSGFNELNEKALAKGEKTFANPRNAA
                     AGSLRQLDPKITRQRPLVLNAYGIGVYESDDELPATHFERLQWLKSLGIPVNNEIRLV
                     QGKAALLAYYAEIQAKRPTLGYDIDGTVLKVNDIALQEQLGFISRSSRWAIAYKFPAQ
                     EEMTILKEVDFQVGRTGAITPVAKLEPVFVAGVTVSNATLHNGDEIERLGIVIGDTVI
                     IRRAGDVIPQITGVMLERRPENAKKIEFPTACPICHSAVTRVEGEAVARCTGGLFCEA
                     QRKEALKHFVSRKAMDIDGVGEKLIEQLMARELIETPADLFKLDHTTLMRLERMGEKS
                     AQNALNSIEKAKNTTLPRFLFSLGIREVGEATALNLANHFGSLEAIRQATFEQLKEVQ
                     DVGEVVANRIVSFWQEPHNVEVVEDLIAQGVRWENVVKAEIVDNPLKDKNVVLTGTLT
                     QLTRDEAKALLQSLGCKVSGSVSSKTDYLIAGEKAGSKLAKAQELGIQILSEQQFIEL
                     TQH"
     gene            complement(31094..32362)
                     /locus_tag="PXB27_000361"
     CDS             complement(31094..32362)
                     /locus_tag="PXB27_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HDL5345191.1"
                     /translation="MTQQIEHSTWQSKFAAMGPGILMASAAVGGSHIIASTQSGAIYG
                     WQLAIIILLANLFKYPFFRFGVQYTLASGKTLLEGYKEKGKFYLIIFFILNVFAAMIN
                     TAAVAIVSAAILKFIFSALGIPLDLSIPMAGTIVIVVTWGILLLGQYKFLDTLSKWIM
                     IALTISTVSAVIIAALKGGVEIAPDFVEPSPWNLAALVFIVALMGWMPAPIEISAINS
                     MWVIAKKRLSKVNYEDGIFDFNVGYIGTAILAIVFLALGALVQYGSGEKVEQAGVAYI
                     AQLIKMYAFAIGDWSKLLIAFIAFMCMFGTTITVIDGYSRANAEALRLISGRKQHSQT
                     ELSIWMTVTALIGILIISQFMSDVAKMLQFAMISSFVSTPVFAWLNFSLVLKGEHRAK
                     GWLFWLAVIGLIYLTSFTLLFLAQQFKLIG"
BASE COUNT         9805 a         6975 c         6212 g         9380 t
ORIGIN      
        1 gacagaaaaa aagccacata aagtgtttta tgtggctttt gcgttaatcg tttagattat
       61 tcaactctta agatacgaca agtatttgta ccgccagctt ttaattcatc tccgtgagtt
      121 aaaagcacta attcgccggt taataaatag ccttgttctt ttaattgagc aatggctttt
      181 ttcgcccctt ccattgtgcg gctgtcttca ttgtaatata ccggtgttac accacgatat
      241 aacgcacaaa ggtttaatgc tttttggtta cgagacatcg cataaatcgg taaacctgag
      301 ctgatacggc tggttaattt agccgtttcg cctgaatgag ttaatgaaat aattgccgat
      361 acaccttcta aatggtttgc ggtgtacatt gcagacattg ccacagcttc ttcgatagat
      421 ttaaacgtac cttccatacg gtggcgagaa atgttaatgc ttggcattgt ttcagcacct
      481 aagcacactt ctgccattga ttttaccgtt tcaacagggt agtcaccgtt tgcagtttcg
      541 ccggaaagca ttaccgcatc agtaccatct aacacagcat ttgcaacatc catcacttca
      601 gcacgggtcg gcattggttt tttaatcatc gattccatca tttgggttgc ggtaattacc
      661 acacgattta gcttacgagc acggcggatt aaacgttttt gcacacccac taaagccgca
      721 tcaccaattt caacccctaa gtcaccacga gcaaccatca caacgtccgc acctaaaatg
      781 atgtcgtcca ttgcttcttc agtcgcaaca gtttcggcac gttcaacctt cgctacaatt
      841 ttcgcttcta agcctgcttc ttttgctaat tcacgtgcat aatgtaaatc tgcacttgat
      901 tgagggaatg aaaccgctag gtaatccaca ccgattttcg ccgctaattt aatgtcttct
      961 ttatctttgt cggttaatgc aggagctgat aaaccaccac ctaatttgtt aatcccttta
     1021 ttgtttgata acgggccacc aactgtcact tcagtatgaa ctttaacgcc ttccacttcc
     1081 aacactttca gttgaacatt accgtcatct aacaatagaa tatcaccagg tactacatca
     1141 tttggtaagt ttttgtaatc taaacctaca gcttcttgat ggccttctcc acgcggtaaa
     1201 tctgcgtcta aggtgaattt atcaccaata tttaagaaaa ttttaccgtc tttaaatgtc
     1261 gaaacacgaa ttttaggacc ttgcaaatcg cctaaaatcg caacgtggcg acctaatctt
     1321 gcagcaattt cacgcacttt attcgcacgt tcgatgtgat cttcaggaat accgtgagag
     1381 aaattcatac gaaccatatt tgcaccggca gcaatgattt tttccaacgt attgccacga
     1441 tctgttgcag gtcccattgt acaaacgatc ttcgttcttc tgagttttct agacattatt
     1501 tgactccatc aataaattta aaaattaagt taaaatcttt ctaagcatct tgattatgcg
     1561 ttaaacgagg gtcattatac gcctaaatca agagagttta aatacaacta aaggagaatg
     1621 ttatgaatat ttggattatg cgccatgggg aagcaggctt taatgcttcg agtgatgctg
     1681 ctcgctgttt aaccgacaat ggtataaaaa acaccatttc acagggaaaa tggttaggag
     1741 aacatttagc caaccaacaa attcaattag ataaaattct cgtcagccca taccttaggg
     1801 cgcaacaaac gcttgaaaat gtgcttattg gtatgcaagc ggtcaatttt ccccaacatt
     1861 ttaccaacct cattgaaagt tgggacgagg ttacgcctga cggaaacccg catacagtag
     1921 aaagctatct acatttttta agagaggaag gcgcaaaaaa tattcttctg atttcacatc
     1981 tcccacttgt gttcgatctc gtccaaattt taacgaataa ccaaagcaat gtacaatttc
     2041 caacttcaac catagcagaa attaattggg taaagacaaa agctgagcta attcgagtaa
     2101 agcatactta attctttcat ttttagaatg tcaatataaa aattttcaat ttttttataa
     2161 attgtcgaat ttatagactt ccaaaatacc taaaaaatgc gtatcatacc agccctcaaa
     2221 tcagtactaa actgatccat aagctattat aagaaagtac ggtatttacc gtacttttta
     2281 tttggatatc cctctggtta aaattacttc aaatttgtta ataaaaggtt aaaaatgcaa
     2341 ataggacaat atgcaatcaa aaatcgtatt ttccttgcac ctatggctgg aatcacagat
     2401 cagccttttc gccgcttatg tagccaactt ggagcaggtt taacattttc agagatgatg
     2461 tctaccaatc ctgatgtatg gcatactgag aaatcacgat tgcggttagc acaccacgat
     2521 gaaataggct taaatgccgt acaaattgca ggttctgatc ctgttgaaat ggcaaaggct
     2581 gcaaagataa atgttgagta tggtgccgat attattgata ttaatatggg ttgcccggca
     2641 aaaaaggtga ataaaaatat ggcaggctct gctcttttac gtaaccccaa attagtcgct
     2701 caaatattag attctgtagt caatgccgtt gatgtccctg tgacactaaa aattagaaca
     2761 ggttgggatc aggaaaatag aaattgtctg caaattgcga aaattgcaga acaagccggc
     2821 ataaaagctc tcactattca cgggcgtact cgaagctgtt tatttaatgg cgaagctgaa
     2881 tatgaaagta ttaaggcagt aaaagaagca atttctatcc cagtgatagc aaacggagac
     2941 attgactctg cggaaaaagc aaagtttgtt ctcgactata ccaaagccga tgcaatcatg
     3001 attgggcgcg ctagctttgg aaatccttgg atatttaagg aaattaatga tttttttgaa
     3061 actggattat cttcaccatt atcaattgat aaaaaatacc aattaatgtt taaacatatt
     3121 gaggatttgc accaatttta tggtgaagaa aaaggttatc gtattgcacg aaaacatgtt
     3181 ggttggtata ccgaaaaatt aaatccggaa tcaaatttta aacgcacttt taatacacta
     3241 agttccacaa aagagcaact ttatgcatta gaagattttg ttaaatcgat tcatttagaa
     3301 tctcaattaa gaaaagttgg ataaaaaaat gttagagcaa caacctacac aaaacccatt
     3361 aacagtaaca atgttaaatg cacaagcgca gcaggttaat aaaccacttc gcgataatgt
     3421 gaaagcagca ttaaaaaact atttatctca attaaatggt gaagatccaa cagaattata
     3481 cgaattagta ttatctgaaa tcgaacaccc aatgttagat atggtgatgc aatatactcg
     3541 cggcaatcaa actcgtgcag caacaatgct aggcattaac cgtggcacat tacgcaaaaa
     3601 attaaaaaaa tacggtatgg gttaatctaa ccgaccgtta aaacacgatt ttaattcgta
     3661 tgtaattagt tagagccaac aaaaaacgct gaatatacta ttcagcgttt ttctatttta
     3721 ttttgccgtc taaaacttca tttcaaatgc taaactgaaa tttctccccg gagcggcaaa
     3781 tcgagtgtaa ttgctacccc ggtcttggtt tactgcattc acaccggatt ggcgcactga
     3841 ttcccaagtg gaatatttac gattagtcac attataaatt cctccacgta aggtgtagtt
     3901 tttataattg atgtaaccgg taatatcatg ggtgtaccaa gaaccggtca gtttgccacc
     3961 taaatcaacg cgatgtatgc cgtgatgacg tgtgcctgcc acctcatcgg cgtttttggc
     4021 tttagaatag gtggtagtaa tgccaattcc ccatttttct tctgggtgat cgtagccgaa
     4081 tccaagcaca tagcgtgctg gctgaatcgc atctagaata ggatcgttta cgctatcgag
     4141 tcttgagtcg gttagtttcc gctcttttac ttttacacgg ttataagcaa ctgatgaata
     4201 aaaaccatct ggcagcatag ataaaatacc gtgtaagtca aatttagcga ctaaatttat
     4261 cccgtttagt gctacatctt gaatattatg gtagttatag aaacagtgtg atttttgtat
     4321 tattctttta catgctgtcg caatcatatt tttatagtta ttacggaaat agctcacatc
     4381 aaggtaacca aattgacctt taaagctaac tccaatctca tgattagtcg atttttctgg
     4441 ctctaactgg tggctacgtt gcgcgcgttg cacatattcg ttatctttaa gcccaatatg
     4501 attacgttta ccataaagtt catagaatgc aggcactcta aaaccgtttg aaatgcgata
     4561 agaaagcgat acaaactctg ttggtttaag cgtaatccca ccattccaag accaatttcg
     4621 ataagttcgg ctaagtgtcc acggatcatc agatttaaat cgatgttgat caaaacgcac
     4681 ccctaatccc aaatccacat actcgcttat cactagatcg cggaagctaa caaaatggct
     4741 atcgccttta attttacgcg gttcacagtt taagatgtcg ccgcgtgtat tattacacaa
     4801 agataccgtt tcaatactgc gtggatcgcg gcgataaata tatggattat ctaaacgccc
     4861 tctaccgcgg tagctggtat aaccgccttt ggtatattgg gcagtcatat ccccatgatc
     4921 cataagcgaa ttaaatcgat caaagcccaa ccctaaattc agtttgtggg tttgcttaaa
     4981 tacgccttga ccagcattta gcgctttatc aaattctaaa tgaatgacac gatgcttttc
     5041 ttggtaatta ttacgctcag ttcgatacat agaccaagat ttatccaaag tcgggcggca
     5101 atttttatct accacaggat aatcgctaca atgcaagcga tgtagccggc tatatagttc
     5161 aatatcttgt ttatccgcac tgagtttaat gctatccaac cagcgattat tctctggttt
     5221 atatttatag gtgaatccta aacgacgttt gtggtgacgt tcatcaaaaa acttcacatg
     5281 gctatagcgc aacccataag ggatccttgg cttgaacact aagccatcta aaatattatc
     5341 cccttcataa acatagttcc taagtgataa gttaatatcg tcttttgtat agtaagctgg
     5401 cgtttgcata tcacggatat cgtagcgttg ttttgtatct tctaagattg cgccaagata
     5461 atgagacgag ttaaagtgat agccaaactt cataaaaaca gaattactct tgtaatctaa
     5521 agggtttggt gcaatgcgat ctttaccggt atattcttgg gctgagagct gctcagtgcg
     5581 ataaggaatc tgctcaagct gtttgcgctc ttcaggcgta tattccggaa atgttctcac
     5641 tgggaaatta tcgcggttca acttaaccct tgcacgagga gtacaatcta atgttgggca
     5701 agtatcttcc acaataaaaa aagatcctcc aggagcattc tgtgcggaat ttgggtaaag
     5761 atcgtagcga ttttcaaagc cggttatacg ccgaatatta tgttttaatt tatttgcctc
     5821 ggaatgaatt ttggtctctt taccgtgtcg gtgagttgca ataacaagtg cttcaaaacc
     5881 acccgcctca ccagccgctg cgatagactg taaaaaatgg ctatttttgc tggcataaga
     5941 ggtcttacta tctaagcccc aatgctgccc ctctttaata atatcctgcg catctttagt
     6001 acgaaaacca atagcaccac ctagcgcacc agagccatat tccgcagaac ttgctccttt
     6061 gcttaactca attgaacgaa tgttttcata ctcaatctca ttaattgcac caccattagc
     6121 atctgaacct agcgtatgat aatgctgcgc ttgtggtagc ccatcaacaa gtaagctgac
     6181 acggttttta tctacacctc gaatggcata gccactactt gcaccgcgac cttgttccac
     6241 caccgaaata ccagggtcat agcgagttaa atcacgaata ccaagaattt gatttttact
     6301 catttcgtga taatttttca ctactttccc caagccagtt acttcgtttt gacgttcgtg
     6361 agcataatgg ctctctgtca caataacttc atccagaaca gctagatcgt tattttcttc
     6421 tattttttta ttttcagtcg cagcaccgta tgaatgagaa agagcaaata acactgttaa
     6481 ggcaacaggt gaatagcgaa aatgatgata tttcattatc attagatagg gtctccttta
     6541 tttaaatttt gcaaattttt ataaaaaatt gcccgcttgc tatctacaac cagcaaaggg
     6601 gaattatgat tatttttcta tttgacgttt tccgccgaat gtaatactga ctctatcttt
     6661 atccgtttct gcactatgta caataccacc taattccgtc gccttagggc cataaaagcc
     6721 cccttcaaat tgagtattta tatgcactac cgtgccacca ttcgtacttt tcggatcaag
     6781 attaaaacca gaattgcccg ttttggctgt gcctgcaaaa ccattccctt tcagattgcc
     6841 ttcaagtgtc agcattgggc gttcttcaac accatcatta gcaatcagct tgccattaat
     6901 ttttttattg acaaaatcta catcgaatag tgaacgagta ccactttcgc tgttacttgg
     6961 cgattcagcc cctgccgagc cagatttact gtacattacc ccgtcccaag tacctcgata
     7021 ttttactgtc cctgttggca aatctgccac agctgtacgt tctcctacta aatattggtg
     7081 agcagtatca gtatcactat tatttccctc gctataagtc ccaaatttga cataatctaa
     7141 attattacag cagactgaaa ctcgatacgt tttaccatca atagtacggg taaccaaatc
     7201 atcaaatttc atctcggcaa agcttttttt gccttccgca ataagtggaa tctgcttatt
     7261 gttaataatc aaatgtgtcg cattcccgaa agtatccatc tcagatttat ttaaatcttt
     7321 aagtttaatt ttgaaagcat caaaggcaga ttcactatca tcttttttag catctcgttt
     7381 gccagcaaaa acaacaaata ccgacttatc atcagctaag aatttaccgg caagttcttg
     7441 agccttccca ccaaaaaatc caccttcaag tgtcgtagag cttttaccaa aataaggatc
     7501 atcggtattt tttgcgtttg ctttaccaga aaagcgatta cctttaattt ttgcatcaat
     7561 atcgtaaatt gttgtaattt tttgctcttt gctacgacta acatacccat tacgaaccaa
     7621 tgttccagtt aaagttttat tagcaaaatc agccgtaaat tctcctgtat gccccattgg
     7681 tttttcagaa tcatcaacat taatatcgta actattttcc ggtgtggcgc caatattgat
     7741 gcctgcggaa ttagaaaaat atttactatc tctccctttt ctcgcatcgg tagtaaaatc
     7801 ccaatatcct ttataagtag ctttttgagt aggtattgct tttgccggct ctatacctaa
     7861 atagtaaaca tacccatgtg cacctgttct caaaatttct ttaggcttta tttcaggttt
     7921 tgcaccagaa tgcagaacat agccggaacg aacaaaatta aaatctctag tatacaactt
     7981 atgatttcta tcatgagtat ggctaaatcc atcagcacta ctaccgtacg ctttaacctc
     8041 ttcttcatac ggaatttttg tcagatctcc agtaatctct tttatatcac caataggggc
     8101 tacatcttcc ttcccctgcg gaaagagatt acgacgcgga attttggctg aaaaaccaaa
     8161 ccccggctgc aataaagctt cagtttcaga agcgcttact gtacgtcttg cgttactatt
     8221 atcatcttgt aaacttggct ttttgggggt agtttgcgtt tgagattcaa ctttggcaga
     8281 ttgaacatca aagcttccac catttgagga acaagcagca agtagcgccg cattaagcag
     8341 tacaaaacta cttttaagtt taaacataat gtctcctttt aatttataaa ataataatca
     8401 ttataattta ttatataaac taatgtaaag gtttgaactt tattagtttc aggatcatgt
     8461 taatcatttc aaggtggaaa cgcttgtttt tagattagat aaaaaataag gagggtcgaa
     8521 atagcgggta tacagaaagt gcttatagcc aaaaaatagt ctattgttat tgaatacaag
     8581 cggtctattt tgtcacattt ttgccaaatt tggttaaaaa accaaccgct tgtaatctta
     8641 actgaagata gatagttttt tattttttct acaactctac agggctggat ttattgcaga
     8701 atggtacttg ccactaacta tcatcactcc caggataatt tatagaaaca atacgagaaa
     8761 ttgtgttaat aaatgaaatg aataacgtta atccgataat ggtgggaaac cacctaaatc
     8821 ttttaaacga ttgaccattg cacagaaaag ttctgccgtg cgctcggtat catacaacgc
     8881 agaatgcgct tgcttaccgt caaacgggat ttttgccacc tggcaggctt ttaccaacac
     8941 ggtttgccca tacataaaac cggcaagggt ggcggtgtca aacatcgcga aagggtgaaa
     9001 gggatctcgc ttggcattaa ttcgtttaat cgcagcctgt aaaaagccct gatcgaacgc
     9061 tgcattgtgg gcaacaatca ccgcccgttg gcagccttgc tccttcattg ctttgcgaac
     9121 cattttgaac atttccggaa tcgcaatatt ctccgcaacc gctcctctta gagggttgtc
     9181 aatatcaatg ccgttgaatt ttaatgactc agggttaata ttcgcccctt caaatggcgc
     9241 aatatgaaaa tggcattgtt gatccggcac tagataacct tgctcgtcca tttttaaagt
     9301 aattgccgcc aattccaata aggcatcagt ttgggcattt aagcctgctg tttccacatc
     9361 aatcacaacc ggtaaatagc cacgaaaacg atgctttaat aatttataat ctatttgttc
     9421 cgttgtctgt tccatttatc tatcaaccta aaatacaagc ggtctgattt gcaaaatttt
     9481 ttacaaatcc gaccgcttga atattcatat tcgcaaaaat tagtttccta acgctgcttt
     9541 cgcatcttta ttctcaataa attcgatttt ataaccgtcc ggatcttcag caaaggcaat
     9601 cacagttgtt ccacccaata ccgggcccgg ctcacgggta attttaccgc ctgccgcacg
     9661 cacatcttca atggttttgt aaatatcatc tacgcctaac gcaatatggc cataagccgt
     9721 gcctagctca tatttatcca caccccagtt ataagtcagc tcaatcaccg cactttcgct
     9781 ttcatcggca tagcctaaaa atgctaacga atacttatac tgctcgttct cgcttctacg
     9841 taacagacgc atacctaaca catcggtata aaacttaatt gagcgctcta aatctccaac
     9901 tcgtaacatt gtatgtaaaa ttcgcatatt ttcttccccc tgaagttaaa aaacgacccc
     9961 tattatggca taatcttggc ttaaaatcat tttctttata ccttttggga ttatctatgg
    10021 ctcacgatca cgattcgccg tcaaaacttg aaatttcaac ccacgctgca ctgccaatgg
    10081 tattaatgct gtttattgaa atggctctta acttctactc tgcaccgaaa aattcaattt
    10141 ttatttctcc ttacttattg gcgtttttcg tgacctcatt aatcagtttt ttagtgctat
    10201 ggaaaggcga aatctgccga gggcaagcca gccgtttaag ctttatttta ccctttttac
    10261 ttattttcgc cgtagggaat ttcctttatt ccataggctt tacgccaaag cataacccaa
    10321 tggcggtggc aagcattgct gcagtttttg cctgcttaat ctacttccgc ctgccggaag
    10381 atcaaacctt atataacaca ttgatgtatt gtggtttggc tatatcaagt attggtatta
    10441 tccaatattt agccatttat tggtttgaac tgccttcatt gtttaacggg attcgagcca
    10501 acaatttcgc ccaagtgctg ttaggcattt tattagccgg ctggtattta atgttagcga
    10561 aaagccgttt agatgttttc ctcaaactgt tggtgaaaac cgcccttgtg gtgctgattc
    10621 tcaattattg ctggacagct tttgtgcttt atcaacatct acaaattatg ccggatatgc
    10681 cattgctttc atttatcatc tattttgcag cacaatttgt gattttattg atgttggcgt
    10741 ggttattact tggaaaaaac atcaaaaatc cgaccgcttg gacagttgct acgctgttag
    10801 cgattttata ccctttcgct aatgtagtct gatttatgaa acgggcatta aaatttttcg
    10861 gcattgcaac cgttctatct gttgtggcaa tctactattt tttaatgccc agccactatc
    10921 ttgccacgcc gattaactgg attttgccga aagcgtggaa agttcaactc ccacaaggct
    10981 taaacctgaa ttggcaaggc tcatcacttg ctcaatttca actctcttat cgagattgcc
    11041 cgctactaag tgccgataaa accgatttgg gttggattga gaaaaaccat ctttctgtcg
    11101 ataaattgga tattgattac cgctgccttg ccttgtttcc acaaaatgaa cacagcaatt
    11161 ccgacacttc actcaacgca ttgctggcgg tcattcctga tggcgaagcg actatccacg
    11221 cccttaactg gaaaaatttg cctaccgatt tcaacccaag acttcagcgg ctttttgagc
    11281 aaacagctat taaactctca ctttcaaaag gtgaaattca ggcaactgcc gctcaacaag
    11341 cggtcaaatt tgcagcaaat tttgcaaatg ggcggttgaa tgccgattta tcatatcaac
    11401 cgaatgaaaa agaacagcat aatcttagga tttcagcaga aattgaggac aattttaccc
    11461 aactgccgcc cactatttcc gctgaatatg attggcaatt atcttatgaa atcatcgcca
    11521 ataaagcctt gcaaaagggc gctctattct cagttggcaa aaaaatgccg cacaaaagtt
    11581 agaaggcaac tggcaggttg aacttgccga aagtgaaaat aacaaactgg atttcccttt
    11641 tctgtttgat ggggaaagcc tagaaattca acaagggcgg tttgattggc actttaccca
    11701 acaatttcca ctgcaagggt ttgttagcac caagctcaca ccgaaatcat tcaaccaaaa
    11761 cggctttttg ccactcaaaa cggcgattcg tatcagcctg ctttcccaaa acagttgggg
    11821 taaaggcaat attgtaatct ccaattcaga cggagaaata accgaaaaag ggctgaattt
    11881 accgcttcgt ttgacgggta atatcaaaca cggcaatttt attctttaca gtagcgtgcc
    11941 actggatttt caaggtaatt ttgacgattt aagcgtaaaa tttttgccta gctccctact
    12001 tcgtttgacc ggaaaagagc ggtatttaac cattgaagat ttacgttttc cacttgccgg
    12061 aattaaaatt gacaagcacg gtattcacgg acgactgcac gcaattttaa gagggcaaag
    12121 ccctgatttt aacaatatcg agttccattt agacggacaa gcacaaaatt ttaaggctgg
    12181 agccttagat tttttccaag atcctaaaga tgctaacgca gtaaaagaca gttggaaatg
    12241 gcgaatttgg ggagcatcta tcctcaaagc cttcaacagt aaagtgaatt taagcggcag
    12301 agggcaatgg cataaacagc ttgtgcggtt ggaagaactc aaaggcgatt tagccaccgt
    12361 aaagttagca gatacaacca tttctaaaat cacgcttaac aatacgcaag ctattcgatt
    12421 taatgtcaaa aaattcaccc ttgacggagc ggtaatgttg caatcacctg agattgcctt
    12481 cagttatggc ggtgtgctac ccaaaccacg agtgaatctc gcctttgatg gtgaagtcga
    12541 aaaactacgt tttaaaggag cggtgaatac caccgaactc ggcccactca aactgttcgc
    12601 ccgccgccaa ttaagccaag atgccagcca aattatcggc aaactttatt ggccggagca
    12661 atctgctcaa ggcttccagc cgctttttcc gtttcgtagc caatgggtga tcaacaacgg
    12721 tacaattcgg ggcgaaacgg cgtttagtgc cggcagtaaa aacgggctga ttgccggtgg
    12781 gcatttagcc attcgtaacg gcggtttatc tttacctaat ggcgaagcaa aagggattga
    12841 attttcgctt ccctaccgct accaaaacgg acgctttcat ttcggagcca aaaaaccgct
    12901 tgatgtaaaa attgcccaaa tcaaactggg ggatttagca ttagataacg cttcaatgaa
    12961 actcaacggt tactaccctt ataccaaagc taagccattg aatttaaatc aattatcctt
    13021 tgatttgttt ggcggtaaac tgaatgtaaa acgctttgcc ttaccacaaa ccgaactggc
    13081 ttacctgaat ttagagcgga ttgactttga gcaaattttg caatttatgc aatatcagca
    13141 aattgatctc aaaggcaaag ccaacgctat cttgccgttt tggttaagcg gaaaaccttg
    13201 ctatatttgc gatggcttac tgacacaggc ggaaacttcc tatttaacct ttacccctga
    13261 attgttgtca gaaatgcaga aaagtggtta caccgagcaa attttgttac atttagtaga
    13321 taaaagcacg attaacgatt ttcgcagtct gattaatgtc gggccgaaag gcgatttagt
    13381 attagacgga aaattaaaat tatcttccaa tcaaaataaa tcaaaagtga atttaaacta
    13441 taatcaccga gaaaatctgt ttgatttgtg gcacttaatt aactatggct cacaatttga
    13501 acaaaaatta gaaaattacc tctatcaaaa actggaacga tgataaagaa tggaataaat
    13561 atgaaaccgg ccacatttta ttttgcaata tttttactga atttgaccgc ttgtacgccg
    13621 aaagtacagc ttgaaacgcc aagtgaaggc attacgatta atatgaatgt ggtcgttgat
    13681 caccgcattg atgtgaagat ggacgagaaa tcccgagcgg tgatcaccaa aagtgagaac
    13741 aaaacgaaaa attagctgaa gataatcatt tgatgatctt taaattgtgc ataaatatcg
    13801 gctaattttt cccctgtcgg tagtactaaa tcaggcgtta aatcaaacat cggcacaatc
    13861 acaaattccc gatttttcat ctcgatatgc ggcacgttca aacgctcggt ttgaatttgc
    13921 tgattgccgt acaaaataat atccagatct aaagttctct ccccccaacg gcgtacacgc
    13981 actcttccct gctcattttc aatgctttgc aatttatcca gcaatgcagt gggcgatagg
    14041 tcggtactaa attttgctac cgcattgaca taatcaggct gatcttgcgg tccgacaggc
    14101 tttgagccgt agaacgggct gatttcaaac tcatttgcaa atttttttaa tttttcgacc
    14161 gcttgtttaa gctgctcaag cggattctca aggtttgatc ctaaggcgat atagacggtt
    14221 tgcataaaat atacttacaa atataacaat aaactctctc cccttgtggg agagagaccg
    14281 cttatgagcg ttcagcgaaa taagcagaga gaggggacaa gcggtaactt ttattaaatt
    14341 ttttacacat ttcccctctc cctgattttt cttctgaacg aagaaaaatc gctccctctc
    14401 ccacaagggg agagggaaat atattaatta tccatttact ttcggttttt tcttaaaggt
    14461 tctacggcgg cgtgttttct ttttctcgct aggcacattg cctaaatgac gaaccgattt
    14521 cagcaattca gtacgctgac cttcattgct aaattggtat tcgtgccacc aagcggaaag
    14581 ttcgaccaaa tcgccgcctt caatttctgc acgcatcact aataaatcaa accctgcacg
    14641 gaatttgacg tgttcaaggg tttgttgcgg tcggttgcca cttcgtttca tcatctgaaa
    14701 ttgcaatgcc caaatatctc gaatgactga agtgtgtctg cggtgcaacg caatcgcgtt
    14761 gcaactttcc gccagcacat catttgctgc cagcatcatt gcatcgtagc tgttgaagcc
    14821 accttcgttt ttaagctgct ccattttctc ccggagcgga taccaaagta gtgcagcaaa
    14881 taaaaacgca ggatttaccg gtaatttatc ggcaattcgc tcatcagttg aatttaacgc
    14941 tttggtaatc attctttcgg cataggaatc acctttggca gtaaaaaatg ccggcaatac
    15001 cggaaacaag aattgccata gttcatattc acgcaaaagc tgataagttt tcagccctgc
    15061 accggtttgt aataatttga gtgattcgtc aaacaaacgg gctgccggaa tgttggttaa
    15121 caacaccgcc atatcccgaa ttggggaggc cgtggcttta tccaagaaca tatccaactt
    15181 cgccataaaa cgaacggctc gcaacattct caccggatct tcttggtaac gaatagtagg
    15241 ctctccgata agcgaaagtt tacctgctcg taagtcttca ataccgttga agaaatcaaa
    15301 aataagattg cgttttatat cgtaatataa ggcgtttacg gtgaaatcac ggcgctctgc
    15361 atcttcacgg agcgtgccgt aaacattatc acgcagtagc attccttcat cgctgacttt
    15421 agaaattttg tcattgcgat gagattcgtg tcctgcacgg aaagtcgcca cttcaaaaat
    15481 ttcacgcccg taaacaatgt gtgccaaacg gaaacgacgt ccgattaatc ggcattgcct
    15541 accaaaaata cgctgaattt cttcaggtct cgcattagtc gcaatatcga aatctttagg
    15601 ctttttctcc aagagtagat ctcgaataca gccacccaca atataagctt cataaccggc
    15661 aatctgcaat ttatcgacaa tcgaaatcgc atttttagga aaatccttcg ggctaattct
    15721 atattggctg gcattcaccg caaatttctt gtgtaacaaa tgggagggag gattatcttg
    15781 ttgtttcttc ccacctctat aagtcttttg gtccgttaag ttcggggctg ggcgtttaga
    15841 acgggaagtt ttgggttctc tttccgctct tttttcactc tctacctttt catttttctg
    15901 agccgacttt ttaggcttgg aaaataatga tttaatataa ttaaaaataa tacacctcta
    15961 aatagtaaat gcctcttaaa aaagaggcac cctgtaaagt tgattataag cctagctgct
    16021 tttctcgaat ttcagctaaa gttttacagt caatacataa atcagctgtt gggcgtgctt
    16081 ctaaacgttt aatcccaatt tctacgccac aggtttcgca ataaccatat tcatcttctt
    16141 ggattttata caaggtttgc tcaatttttt tcaataattt gcgttcacga tcacggttac
    16201 gaagctctaa actgaactct tcttcttgac tggctcggtc gttcggatcg gcaaagttag
    16261 tcatatcttc ttgaagctgg cttttggtac gctccgcttc ttccataatt tgactgtgcc
    16321 acgctttcag aattttggtg aaatgctcac gttgagcttc attcatatat tcttcgtctt
    16381 ttttaagctc ataaggctca acgcctgcta atgctaataa accgagtgag gttttagccg
    16441 cttgagtcat agatatttac tccttgttcc ctgtttattg gaagttttat tgttaaaatt
    16501 attgcccgaa tacagactaa cttccttgta accgcactat acgaaaaaat caacatcttc
    16561 gggttttaaa taatggcggc tataaataac acaaaaacga ctcattaatc aaatgaattt
    16621 gcaaaaattt tgcattcaaa ttgatttgat tgcacaaaaa aatagccgaa catccattcg
    16681 gctatttttc aaacactttt taccagctct ttgaccaatc ggctaaccat ttttggatag
    16741 tttcagtagt cggtggcgta aattcaatgg ttttataggt tggcattgcc gcaaaagccg
    16801 gatcaacgcc gtcggttaaa accggattca ttacgttgtg gtaagaaata atatgttgcg
    16861 attccggttt gtgcaaaaat tcaagaaatt gatttgccaa tgctttttgt tggctgcttt
    16921 tgacaatagc cgcattttct acttgcacca aatgcccttc ctcaaacgga gcagccacat
    16981 atttatcggt gttttcgtgc cacttatgat ataaaggcga tgtagtatag cttagtacca
    17041 aatccgactc gccttttaag aacgctccat aagtttcaga ccaacctttg cctaccgtta
    17101 ccgtatgttt tgccaatgtt ttccaagcag ttggagcttc atcgccatac acttgattta
    17161 cccacattaa gaagccccgc ccaacgctgc tggtacgtgg gtcttggtaa atcactttta
    17221 aatcttggcg ttccaccaac tcttttaaac tttttggtgg atttttcagc ttctctttat
    17281 cgtaaataaa agcataagaa cccgtgtcat aagggaagaa aattttctgt tgggtttcta
    17341 atttggtctt tgcttcaaac tcggcaaaca agccactttt ttcggcttca ggttgcagga
    17401 aattatcaat accgaccacc acatcagctt tggtttttgc tccctccaaa cgcaagcggt
    17461 tgaacatcgt tacgctatcg tcaagcggaa caaatttgac atcacagcca cactctttct
    17521 caaaattctg ctctaattta ggagcaggtc catattccga agcaaacgag ctgtaagtgt
    17581 aaacggttaa ggtcggttga ttggcataaa cacttgcact taataacgca agtgaagaga
    17641 ggataaattt cataacaatt ccttctaaat taaatgaata gaaggattta agaaaggcaa
    17701 gttgctatct caaatcccta cgctgatatt aatcagatca ggttctatgg gtatttctca
    17761 gccacaagcg gttaaatttc ggcaaaaatt tgcaaatttt tacgcaaaaa caaccgcttg
    17821 ttgcacccca atgagaacgg cttcattgta aaaagtttgc aggcagattt caaccaaagc
    17881 aaacgtttgc tatttgtatt aaaaatccgt atactaccgc cctagtccaa acaaggaact
    17941 agaaatgaat tgcacaacat cggttaaatc aaattcccaa cagctacgct tcaactttag
    18001 aggcgtagct gtttttctat tcatttcaaa ccattcaaaa tcgctgttat agcgattttt
    18061 tttcgcacgc ttgcaattac cgcaagcggt tgagccacat taacagagag aaaaggaata
    18121 aaaatgagcc aattgattcg taccctaaaa ggacatattc gtgatgaaat catcaaaaaa
    18181 ggcggttggg taaatgcaca cgcccacgca gatcgtgcat ttacgatgac gcctgaaaaa
    18241 atcaccgttt accaaaatgc gaatctacag caaaaatggg atttggtcga tgaggtaaaa
    18301 cgtaattcaa gcgtagaaga ttactaccgc cgcttttccc aagcgattga attgatgatt
    18361 tcacaagggg taacggcatt tggtactttt gtggatatcg accaagtatg tgaagacaga
    18421 gccattattg ccgctcacaa agcccgtgaa gtatataaaa acgacatcat cttgaaattt
    18481 gctaaccaaa cgttgaaagg cgtgattgaa cccaatgcga aaaagtggtt cgatatcggc
    18541 tcggaaatgg tggatatgat tggtggcttg ccttaccgtg acgaactgga ttacggcaaa
    18601 ggcttggaag caatggatat tttaatggat aaagccaaat cgctcggcaa aatgctccac
    18661 gtccacgttg atcaatttaa ctcacctaaa gaaaaagaga ccgaacaact gtgtgataaa
    18721 tcgattgagc atggtatgca agggcgagtg gttgccattc acgggatttc catcggctcg
    18781 cacccgaaag aatatcgaaa aatgctttac caaaaaatgc gtgatagcca aatgatggtc
    18841 attgcctgtc cgatggcgtg gattgatagc ggacgtaagg aagatttaca accgttccat
    18901 aacgctttaa cccctgctga tgaactcatt cctgaaggca ttactgttgc tatcggcaca
    18961 gataatatct gtgattatat ggtaccgctt tgcgagggcg atatgtggca agaactcagc
    19021 cttttatcag caggctgtcg ttttactaac ctagaagaaa tggcaaatat tgccagtatc
    19081 aatggtcgaa aagtactggg tttagtctaa ctgaataaac gaaaggcgaa ccctaggttc
    19141 gcctttaata acagcataac cgattattta aacaagttac ccaaaatccc ttttgccgct
    19201 tgagctaata agtcatttgc atcaaagttt tttaagtctt caacattacc attcggggtt
    19261 aaagtatcca cgatttttgg caagaactgg gctaataaat cgctcgcatc agcagcatca
    19321 acacccgctt cttgagccac attttgaatg ttttcttgcc cgaacacatt actgatttgt
    19381 tcgcccgaaa caggtgcatt gtcttcatcg ctgctgatcc aagtgcgtaa cacatcatct
    19441 aatccgcctt gttggaattt ggcaatcagc ccttcaatcc caccttgtga ggcgagtaaa
    19501 ttttgaatta attgagttgc agttgattgg ttattgccac tcaatactga agaagccaat
    19561 gaacctaaaa tatttcctaa cataatttct cctatgcaag cggtacaatt ttgcgaaaat
    19621 tttgcaaaaa aacagcaaac ttcgaccgct tgtaatcact ttaacgagct aaaatggctc
    19681 gcactctttc caaatcttcc ggcgtatcca cccccacttg cggggcttct ttcgccggtt
    19741 cgatatgaat tttttcaccg tgccataagg ctcttagttg ctcaagggac tcaagctgtt
    19801 ctaattgtgt cggctgccac gccacatatt gtttcacaaa accggctcgg taagcgtaaa
    19861 tgccgatatg gcgtaaataa ccttgagcgg caacaaaact gtcatcacaa tccggaaaac
    19921 tgtctctggc aaacggaatg ctggctcggg aaaaataaag tgccattccg tttttgtctg
    19981 ccaccacttt caccgcattt gggttaaata gctcttctcg ggtcgctaat ttcacaccga
    20041 gtgttgccat attgacttga taagcatcta aattttgagc tacctgagta acaattaccg
    20101 gtggaatcag cggttcatca ccctgaatat tcacaataat ttcgctgtcg gcaatctgca
    20161 ttttttcgat cacttccgcc aaacgctcag tgccggaatt gtgtttttcc gaagtcatac
    20221 agacttccgc cccaaaagat tgagcgaggg cttcaatttc agggtgatcg gtggcaataa
    20281 tcacacggct tgcaccggca agctgagctt tttcccaaac gtgttgaatc atcggtttgc
    20341 ctgcaatatc caacaacggc ttacgaggca aacggctgga agcgtaacga gcaggaataa
    20401 ttacagtgaa tttcataggc tatccttatg cttcttgagc atctaagaaa cgttctgcat
    20461 ccagggctgc catacaacct gtgccggcag aggtaatcgc ttggcgatag ttatgatcca
    20521 tcacatcacc ggcagcaaat acgccttcca cagaagtggc agtagcattg ccttccaaac
    20581 ctgatttcac tttaatgtag ccgttttcaa gttctaattg accttcaaac attgccgtat
    20641 tcggtgcgtg cccaatcgcc acaaagaaac catcaacctt aatttcttcg ctcaattctt
    20701 ctttggttga ttttaaacgt aagccggtaa cgcccatatt atcgcctaac acctcatcaa
    20761 ccgtacggtc tgtgtgcaga ataattttgc cttcttcaat acgtttttgc agacgaccaa
    20821 gcaagatttt ttcagcacgg aagctatcac ggcggtgaat taaatgcact tcacttgcca
    20881 aatttgccaa ataaagtgct tcttccacag cagtattccc accaccgact accgcaaccg
    20941 gtttattgcg atagaagaaa ccatcacaag ttgcacaagc cgacacgcct ttgcctttga
    21001 aatgctcttc agaaggcaaa cctaaatatt tcgccgacgc accggtggca ataattaacg
    21061 catcacaggt gaaagtgtat aaatctccgt ttagcgtgaa tgggcgttta gataaatcca
    21121 ctgaatgaat atgatcaaac acgatttcag tttcaaattt ttcagcgtgc ttgagcattt
    21181 tgttcattaa ctctgtgccg gtaatttgtt caaattcccc cggccagttc tcgatttcat
    21241 cggttgtagt gagctgtccg ccttgttgca taccggtgac taaaaccggg tttagattgg
    21301 cacgggcggc ataaacagca gcggtataac cggcaggccc tgagcctaaa attaaaagtt
    21361 tggtgtgttt agtggtcata atgaatcctc tgaataaata ttaatgttta ttttagccta
    21421 aatttcagtt caactctatt ttcgttttcg ctcaactttt ttcaattttg tgacaaagtt
    21481 aacagaaatc cgactgtgtt tcttgtaaat tgtgacagtc taattcattt aaaaggggca
    21541 aaaagatgaa aaaattaatt aattcaatag aaaccgtctt agatgagcaa ctttctggtt
    21601 ttgtgaaagc acattctgat ttaacactta acacagaacc tgtctatatt taccgcacaa
    21661 cgccaagtaa tcaacctaaa gtagccctta tttccggtgg tgggagtggg cacgagccaa
    21721 tgcacgccgg ttttatcggt aaaggaatgt tagacggagc ttgccccgga gcaatcttta
    21781 catctccaac gccggatcaa atgtttgaat gcggtttagc ggttgatcgt ggcgagggtg
    21841 tattgctaat tattaaaaac tataccggtg atgtgctgaa ttttgaaaca gccaccgagc
    21901 tacttgcaga tagcggtgta aaagtcgcaa ccgtgttggt tgatgatgat gtggcagtca
    21961 aagacagttt atatactgcc ggccgccgcg gtgttgcaaa tacagtatta attgaaaagc
    22021 tgttaggagc tgcagcggat aaaggctata acttatccga attagccaaa ttagggcatc
    22081 agttaaacaa taacgggcat tctatcggaa ttgccttatc tgcttgtact gtgccggctg
    22141 ccggtaagcc atcatttact ttagctgaaa atgagatgga atttggggta ggtattcacg
    22201 gagaaccggg cattgaacgt cgcccctttg aaaatctgga tagtaccgtg aaacaaatgt
    22261 ttgagacctt aattgctcac ggcgattatc aacgtaaaat tcgccattgg aatacacaaa
    22321 cccaacaatg ggacgaaatc gaagaaagca aagtggcatt gaaaaaaggt gatcgtgtta
    22381 ttgctttagt taataactta ggagcagtgc cactatccga actttatgct gtttatgatg
    22441 cgttagaaaa agagtgccaa actttcggct taagcattga acgcagtctt gttggatcat
    22501 attgcacctc gctggacatg caaggtatct ctatcacatt actgaaagtt gatgaggata
    22561 ttctcaaact atgggatgct ccggtaaata caccggcaat gcgttggggt gaataattag
    22621 attgaaagga taagttatga caatatcaaa acaacagatc ttaaaatggc ttgaaaattg
    22681 ccaacaggta atgactgaaa aacgggatta tttaaccgaa ttggataccg ttattggcga
    22741 tggcgatcac ggtttaaata tgcagcgtgg atttagtaaa gcacttgaaa aagcccatac
    22801 cgtgcaagat aaagatatcg gcaccctgct taaaaccgtt ggaatgacat tattatcgca
    22861 agtaggggga gcgagcggcc ctctctatgg ttcatttttt attaaagcgg ctcaaggtgc
    22921 tacaggcaaa gagcaactga gctttaacga gttactggca cttctacagc aaggagcaga
    22981 agctatcgta caacgtggac acgcacaagt tggcgataaa acgatgtgtg atttatggct
    23041 gccattactc gaagacttag ctcaagccga tagcactcaa ccaattgctc aaatcttatt
    23101 acaagcggtc gaaaaagcag aaaattttgc aaatcaaacc gctccattaa tggcgaaaaa
    23161 agggcgtgcc agttacttgg gcgaacgcag tatcgggcat aaagatccgg gagcaacgtc
    23221 cagctactat atttttaaag ccttagctga agcattacag taggaggggt aaatggttaa
    23281 tttagtgatt gtttcacata gtgctaaact ggcggaaggc gtgggggaaa ttttggctca
    23341 aatggcacag gggggctgta aaatcgccct tgccggcggc attgaggata cctcaaatcc
    23401 tatcggcact gatgcggtaa aaataatgca agcgattgaa tcggtattta gcccagacgg
    23461 tgtgattgtg tttgtcgatt taggcagtgc aattttaagt acccaaaccg ccatagattt
    23521 actggagccg gaacttgccg aaaaagtgat cattagctac gctcctttag ttgaaggtgc
    23581 ttttgctgct gtagttgcag cctctgccgg cgatagttta gaagcggtat taaatgaagc
    23641 gaacgaagcc gctgctctta agcaggaaca agccaattag taaaaatgcc gtatcgaaaa
    23701 atacggcatt ttagttttat tagtacaata gtgaataaag ctgtcttcta aatttcggca
    23761 caatcgggtc tgtacccagt gcagataaga ttgccagaaa ttggcttttt acctcgccgt
    23821 tcgctacatt taaatccgat tttaaccaat caaaaagtaa cgtgagtgct tcttcatttt
    23881 tatgagcttg gtgtagctga tttgccaatt taatcgcaat ttcaggggtt ttgctgttcg
    23941 cataatccgc ttgaagctgt tgtaattcgg gcgattcgct ggcttgttcc aataattcga
    24001 tttgggcttg caaaccgtgc caacggctgt ctctgtcttg aatcgggatc gctttaagga
    24061 tttcacgagc ctcttccacg cttttcattg caatgtaagt ttcggcatag agcaaagcga
    24121 aatcgctgtt tttcttatcg gtcatttccc acgctgattt cagcagcggc aaggcttcgg
    24181 tataacgctg atcttgcaat aattccaagg cttgttgaaa tttcagctct tcctctttcg
    24241 gtaaaatatt tgccaaccga atccgtaatt cctgctcact tactgcacct tgaattgcat
    24301 caatcggctg accgccggca aacaaataga gcgttggaat ttgttgaatg cgaaattgag
    24361 cagccaatgc ttgttgctca tcacaattta ccgtcgccaa taaaaattgg ttagggtttt
    24421 catccgaaat acgacgtagt aaggcatcca tttccaatga agccacttcc cttggcgaaa
    24481 taaaatgaaa caccgtcggc acttgtgccg ctgctgccca tacttcacta aaattctgct
    24541 cgtttacatt tacaaagtag tccatacaaa accttcttaa gctaaaaatt actttttata
    24601 ttgtagcatt aataagaagt ttcaataggt agcccgtttc tctcccacgc ttcaaaacca
    24661 ccttttacac tataaacatt ctcaaaccct tgttcgatta aaaactgtgc tgtactttgg
    24721 ctactcactc cgtgatagca aatcacaacc accggttcgt cataatcgac ctcgtctaaa
    24781 aacttaccat aactctggtt ggttaaatga aacgcatcct gtgggtggct gtaaacataa
    24841 cggcgggcat cacgcacatc ggcaagcacc gcaccttcgt tttcgattaa ttcccaagcc
    24901 tgttgcgggc taatttcttt aaatgtagtt tccataatca ctttccctca aaaaacgtgc
    24961 aactatacca taaaattcta aaaagaatgg ctattattcc aagttgaaaa tttgatgttt
    25021 gtcagatata attgcaaact attttcaaat gcactgattt aaaactacca cagtttgagg
    25081 attttatgga gcaattattt caatttttag aacatcatac tgactttgtt atattaggat
    25141 tactaggctt attaagcgta atctgccttt ggaaagctat tgaacgtatt atgttttatt
    25201 ccaaagtaga tatttcaaaa tacgataccc tcgaagaatt agacatcgat ttaacccaca
    25261 atttaactac catttcgact atcggctcta atgcccctta tatcggctta ttaggaaccg
    25321 ttgtcggtat tcttttaact ttctatcatt taggtcaatc aggtggtgat attgatgcgg
    25381 cctctattat ggtaaacctt tctttagctc taaaagccac tgctgtcggt atcttagtgg
    25441 caattattgc aatggttttc tacaatggtt ttggtcgtaa agtggaagaa aacaaactga
    25501 aatggttggc attacaatca aaaaagcctc atcaagcggt gtgatttagt ccattttttg
    25561 caaattaata aggcagaaca atgaaaaaat tcgatgaaat caacatcatt ccatttattg
    25621 atattatgtt ggtattactg gctatcgtgt tagttaccgc ctctttcatt tctcagggta
    25681 aaatccaagt aaatgtaccg aaagcaagta caacacagcc aatgaaagca gacgatttag
    25741 cgaagctttt aaccattacc gaaaataatg agtttttctt taatgatcaa gcaataacta
    25801 aagagcaatt aattgctgaa gttgccactt gggataaaag ccaaaaagtg tcactcaaag
    25861 ttgatggtgc agtcgccttc gaaaaatttg tggaattaac cgatatcctt tctgcaaatg
    25921 aaattaaaaa tgtagcaatc attactaaaa aagaaactgc tccagcacct tcaagcacac
    25981 ctggtagccc tgcacaagtg ccagcagtcg caccttaaat agggtaaaca ggtatgaaaa
    26041 aaaaacattc tcgtatcggc ttattggcat ctttggctat tcacactgcc ttaattggtg
    26101 gcacttttgc ttggttacat aaccacaaag tggaaaaaat tgccgagcaa aactctcttt
    26161 ctatggaaat ggtggcagca ttattagagc aacctcaggt tgccgttgct ccggatccta
    26221 ttaccgaaga ggtcaaagaa atcgaaccgg agaaggcaga agtaccacct gaacctaagc
    26281 ttactccgga gccaactcca gagccggagg caattccaga tcctacgcta aatccgaaaa
    26341 aggaaaaacc gaaagaagaa aagcgtaaag agcgacacca ccataaacat aagcatcgtc
    26401 acaaagagaa aagggaagag ctacaagagc aagaaaaacc gaaagataag cctaaagagc
    26461 gcccgaaaca tcatcacaag catattaaag cattagagcg cgggccagag ataaaacaag
    26521 gtattgtcgc taaagcgatt ccaaatgcgg ctgaaggtga aaaagtggtt gcgggggttg
    26581 ttggagggca aaaaaacggt tcaccaaatt caacctcaac tacaggttca gcaaatactg
    26641 gggctagcag tggtaatgcc agtgaacttg ctgcttataa agtcgcatta cagcgagcat
    26701 tacagcgtaa ggcgaataac tcttaccctc agcgtgaaaa aatgatgcgg aaaaccggta
    26761 cagttacgat taaatttacc attacatcgt ccggttcaat tacaaatgta tccgtactca
    26821 attcatcggg aaatagtaat ttagataacg ctgcggtacg cagtgcagaa ggtttgagta
    26881 tgcctccgcc accggcaggc ttccctccta cggtaacagt accggtaaga tttgcccttg
    26941 aataaaaaca gccctcaaaa gcaatgtact tttgagggct ttttattgca aaaaattgac
    27001 taaaaatgac cgcttactgc ttgcgaatat cgccatttct cacattcgga ctcacttcac
    27061 ccgtacgagt ttctgtgctt aacgaggtag aatttacttt cgtaaaagta taatccgctt
    27121 tgctgaattc acctgtttga ttatttaaga agaaattgcg gtactctttt acccataatg
    27181 ccgttgcgcc acaaactgcc acaggcataa ctactaaatt cacaaatgga atcatcgtga
    27241 ataggctcac taaagcacca aaggtaaaat ttaaagtgcg agactgcgac aacgcatttc
    27301 gcatacgctg aaaactgatt ttatggttat caaacggata gtcacaatat tgaatggcga
    27361 tcaaccaagc accgaagata aaagtcagca caggtgctac ggtttgccca accacaggca
    27421 aaaagcctaa tacaaaaaga gcaatcagcc ttggaatgct atacatcatt ttttgccatt
    27481 ctcgtttcag cattcgaggc acatctttta acattgcacc gaatgacata tcgcccaaag
    27541 gctcgccggt aagttgcagt tcgacttttt ctgctaataa cgcattaaag ggagcagcaa
    27601 caaagttagc caaggtagta aaagcaaaat aaaatcccac taatatcatc aaaaaaatca
    27661 gtggaatcag tacaaagcta agccattcca gccagcttgg cagtaacata tccaacaagc
    27721 caccgatatt ggcaaaaaat ccccaaatca gccctaccat cagcacggta ttaataagaa
    27781 ttgggaaaat cacaaaaggc aaaaattgac gttgtaacaa taaccgccag ccatatataa
    27841 aataatgaaa gcctaatccg acttgatttt gcggctcaga aaaaactgaa gacataatga
    27901 ttccttattt tctatcaaaa ttaattagaa atatggtaag ctacgcacat tattttcgca
    27961 aatatgaaaa tacatttttt attgattatt tgataaagag gacacaatgg aactgcatat
    28021 tatttttttt attcttgccg ggctactgat tgctgtacta attggattta gcctttggtc
    28081 cgctcgtcgt gaaaaatcaa atattttttc aaatacattc tcgaccagac ctattgctgt
    28141 gccaccacag aataataaaa tgagcaatag catcccaacc tcattacagc cacaaaatga
    28201 ggattatcaa gcaatgtctg tgccggaaga aaatccgcaa cagatacaac aggaagttga
    28261 aagctcgctg caaaatatca aaatcagttt gccgggcgga aatcctcagc aacagcctga
    28321 gcaaattcag ccgatacaat cggtagcaga accaacggtt agcacttacg gtcagaaaat
    28381 ttctaatcag caacatatcc aatacaatac gccggatttt gcaaacccaa tggtgaacac
    28441 agtggtggaa gaaacacctc aagcagtagc tatcgaacct caagaaaccc aatcggcaga
    28501 ggaagaaaat ccatttgtaa ccctctatct ttacgcacca caaaatgaag ctttccgcgg
    28561 tgattttgta gtacattatt tagaagaagt tggcttacgt tttggcgaac accaaatttt
    28621 ccaccgccat caacatattg atgattcatt aagtccgatt attttcagtg cagcaaattt
    28681 aatgaaccca ggaacctttg atttaaataa tatctcaaac tttgcgacag ctggtttagt
    28741 gctatttatg caacttccaa ccgaaggcaa tgatgtggct aatatgaagt tgttaatcag
    28801 taacgctgaa cacttagcag catcgttagg gggattctta tatgacgata atcaacagcc
    28861 ttttggcgaa gaatcccgcc aacgttatat gcaacgtgtg atgcactaat tgcgaaaaaa
    28921 ttaataaatc cgaccgcttg tacaagcggt cttttttgta gagaatttta ccaatgtctt
    28981 tatttaacca aacctcttta tttgaagaga atgccacgcc cgagttagtc gagaaaatcg
    29041 aaactattcg ccaaacattg cgtcaatacg agtatgaata tcacgtttta gataacccaa
    29101 gcgtacccga tgccgaatac gaccgtttaa tgaacgagct gaaaaatctg gaatggcagc
    29161 acccggaact gattagtgcc gactcgccga ctcaacgggt tggtgccaaa ccgctggcag
    29221 gctttgctca agtcacccac gaaatcccta tgctttcgtt ggataatgcc ttctccgatg
    29281 aagacttaga tggcttttta cgccgtatgg aaagctatat caccgcctct ccttcgcaac
    29341 tggaattttg ttgcgaacct aaattagacg gcttggcagt gagtatttta tacgaaaacg
    29401 gtatacttac ccgagctgca acccgtggcg atggcacaac cggcgaagaa attaccgcta
    29461 atatccgcac cattcgcaat attccgttaa agctcaacac gactaatcca cctgctcgat
    29521 tagaggtgcg aggcgaagtc tttatgccac aatcaggctt taatgagctg aatgagaaag
    29581 ccttagctaa aggcgaaaaa acctttgcca accctcgcaa tgccgcagca ggttcgcttc
    29641 gccagttaga tccgaaaatc acccgccagc gtccgttagt gctgaatgcc tacggaattg
    29701 gggtgtatga aagcgatgac gaactgccag ccacccattt cgaacgtttg caatggctga
    29761 aatcgcttgg tattcctgta aataatgaaa tccgcttggt tcaaggcaaa gctgctctct
    29821 tggcttatta tgcggagatt caggctaaac gcccaacatt aggctatgat attgatggca
    29881 cagtattgaa agtaaatgac attgccctac aagagcaact cgggtttatc tctcgctcct
    29941 ctcgttgggc gattgcgtat aaatttccgg ctcaagaaga aatgacaatc ctcaaagaag
    30001 tggacttcca agtgggaaga acaggggcaa tcacccctgt ggcgaaatta gagcctgttt
    30061 ttgtcgctgg cgtaacggta agtaacgcaa cgctacacaa cggtgatgaa atcgagcgac
    30121 ttggtattgt gattggcgat acggtaatta ttcgccgagc gggcgatgta attccgcaaa
    30181 ttacaggcgt gatgttagaa cgccgcccgg aaaatgcaaa aaaaattgaa tttccgaccg
    30241 cttgtcctat ctgtcactcc gctgttactc gggtagaagg cgaggcagta gcacgctgta
    30301 ccggtggttt attctgcgaa gctcaacgta aagaagcctt gaaacatttc gtttcacgca
    30361 aggcaatgga tattgacggc gtaggcgaaa aactaatcga gcagttaatg gctcgagagc
    30421 tgattgaaac gcctgccgat ctgtttaagc tcgatcacac taccctaatg cgattagagc
    30481 gaatggggga aaaatcggct caaaatgctc tcaacagcat cgaaaaagca aaaaacacta
    30541 ctcttcctcg ctttttattc tcgttaggta ttcgtgaagt gggcgaggca accgctctca
    30601 acttagccaa ccattttggt agcttagagg cgatccgcca agccactttt gagcaattaa
    30661 aagaagtgca agatgtgggc gaggttgttg ccaaccgtat tgtcagcttc tggcaagaac
    30721 cgcataatgt ggaagtggtg gaagatttga tcgctcaagg tgtacgttgg gaaaacgttg
    30781 tgaaagccga aattgtagat aacccgttaa aagataaaaa cgtggtctta accggcacgc
    30841 taacccagct cacccgtgat gaagccaaag ccctattaca aagtttgggc tgtaaagtct
    30901 ccggctcggt ttccagcaaa accgattact taatcgccgg cgaaaaagcc ggttccaagc
    30961 tggcaaaagc tcaagagttg ggaattcaga ttttaagcga gcaacaattt attgagctta
    31021 ctcaacatta acaaacaaag gggcagatga ttgaaatctg ccccttattc tatctattga
    31081 atgcttaaaa gcattaacca atcaatttaa actgctgagc taaaaacagt aacgtaaagc
    31141 tagttaagta aattaagccg ataactgcca accaaaataa ccagcctttt gcacgatgct
    31201 caccttttag cactaaagag aaatttaacc acgcaaaaac aggggtagaa acaaatgatg
    31261 aaatcattgc aaattgtaac attttcgcca catcactcat aaattggcta ataattaaaa
    31321 tgccgatgag tgcagttacc gtcatccaaa tacttaattc tgtttgtgaa tgttgcttac
    31381 gcccgctaat taaacgcaac gcttctgcat tcgctcgtga atatccatca ataacagtga
    31441 tcgttgtgcc gaacatacac ataaatgcaa taaatgcgat taaaagctta gaccagtcac
    31501 caatcgcaaa agcatacatt ttgattaatt gagcaatata ggcaacaccg gcttgttcta
    31561 ctttctcacc tgaaccgtac tgaactaacg cacctaatgc taaaaaaaca atcgccaaaa
    31621 ttgccgtgcc gatataaccc acattaaaat caaaaatacc gtcctcataa ttcactttac
    31681 ttaaacgttt tttcgcaata acccacattg agttaatggc agaaatttca attggtgcag
    31741 gcatccagcc cattaatgca acgatgaaca caagtgctgc taaattccac ggcgaaggct
    31801 ctacgaaatc tggagcaatt tcaacgccac cttttaaagc agcaatgatc acggcagata
    31861 ccgtagagat ggttaaagca atcataatcc atttagataa tgtatctaag aatttatatt
    31921 gccctagaag taaaataccc caagtgacca caatcacaat agttcctgcc attggaatgc
    31981 ttaaatctaa cggaatacct aaagcagaaa aaataaattt tagaatcgct gctgaaacaa
    32041 tcgccaccgc agctgtgtta atcattgcag caaaaacgtt aaggataaag aaaataataa
    32101 gataaaactt tcctttttct ttgtagcctt ccaataaagt tttaccgcta gctaaggtat
    32161 attgcacacc aaagcggaaa aacggatatt taaataaatt ggctaacaga ataataatcg
    32221 ccagctgcca gccataaatc gcaccagatt gtgtagaggc aataatatgc gaaccaccca
    32281 ctgccgcaga tgccatcaaa atccccggcc ccattgcagc aaatttactt tgccaagttg
    32341 aatgttctat ttgttgtgtc atatagcttt cc
//
LOCUS       DANLUQ010000009        31222 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.009, whole genome shotgun sequence.
ACCESSION   DANLUQ010000009 DANLUQ010000000
VERSION     DANLUQ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 31222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31222
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.009"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            310..891
                     /gene="rsxA"
                     /locus_tag="PXB27_000362"
     CDS             310..891
                     /gene="rsxA"
                     /locus_tag="PXB27_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007525505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HDL5345192.1"
                     /translation="MVDYILLVISTALINNFVLVKFLGLCPFMGVSKKVETAIGMGLA
                     TTFVLTVASLSAYLMETYLLEPLNAQFLRTLVFILVIAVIVQLTEMIVHKTSPTLYRL
                     LGIYLPLITTNCAVLGVALLNVNLANNLVESVLYGFGAAAGFSLVLVLFAALRERLAA
                     ADVPRPFQGASIALITAGLMSLAFMGFTGLVKL"
     gene            891..1508
                     /gene="rsxB"
                     /locus_tag="PXB27_000363"
     CDS             891..1508
                     /gene="rsxB"
                     /locus_tag="PXB27_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HDL5345193.1"
                     /translation="MLDLPIIAYILIAIAVIALIFGAVLGYSSIKLKVESDPIVDQID
                     ALLPQSQCGQCGYPGCKPYAEAIANGDVITKCVPGGQPLVVNLAELLGVEVPPMEGMA
                     EPEMKVAFVIEDLCIGCTKCIQACPVDAIIGTNKAMHTIIPDLCTGCELCVAPCPTNC
                     IEMRPIKPTTQSWNWKFDQDLIIPIVNTTELQRKLVTGGGKNEQS"
     gene            1495..3789
                     /gene="rsxC"
                     /locus_tag="PXB27_000364"
     CDS             1495..3789
                     /gene="rsxC"
                     /locus_tag="PXB27_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HDL5345194.1"
                     /translation="MSNPNLVIERIRQNKETGKLWDFPGGIHPMENKSQSNSKPIRTL
                     NLPKFFYVPLVQHSGWAGELLVKVGDVVLKGQPLTKGDHFRQLPVHSPTSGRVIGIEP
                     YVSAHASGLPEITVIIETDGKDKWVERQPIEDFLSLTSDQIIQKVYQYGIAGLGGAVF
                     PTATKLSLADKRCKLLVINGAECEPYITCDDRLMQDFTADLIEGIRILRYVLRPEEVV
                     IAIEDNKPHAIQALETALKGSNDMFVRAIPTKYPSGASDQLIQVLTGLEIPAGKRTIE
                     MGIVMHNVGTAFAVKRAIIDDEPLIERVVTLTGDKIRNKGNVWARLGTPIQHLLEQVD
                     YQADKRFPVFLGGPMMGFILPALQAPITKTANCLIAPDHFEYAPPEPERSCIRCSSCS
                     DACPVGLLPQQLYWFARAEDHDKSKEYHLDACIECGVCAYVCPSYIPLIQYFRQEKAK
                     IEEIEEKARKAEEAKIRFEAREARLQKERDARTARISQAAEKRREEVANAGGVDPVKA
                     ALERLKAKKAVETVQEIPAEVQVKPNGEPDNSDLMAQRRARRLAKQAEQAVEKSENVT
                     NALIGANNDLPLQSMNETASDKSVGANNYSPEAESEDPRKAAVAAALARAKAKKQAQQ
                     AVEKSENITNAVIGVNNDLPLQSMNETVSDKSVGANNHSPETESEDPRKAAVAAAIAR
                     AKAKKQAQQAVEKPEDIANAVIGVNNDLPLQSMNETTSDKSVGANNHSPKTESEDPRK
                     AAVAAAIARAKAKKAAQQEKSNNV"
     gene            3782..4417
                     /locus_tag="PXB27_000365"
     CDS             3782..4417
                     /locus_tag="PXB27_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HDL5345195.1"
                     /translation="MFSELIPALPWSFLFIQDLIGTIVFAISGAIAARQHKMDIFGMF
                     ILAFVTGVGGGTLRDMMIGSTPVFWMKQPIYVLMITLAVVITAIFRNQISRKSWQTGL
                     LVFDAIGLGVFTVIGVQKGLDFGLHPLICVALGGVTGCFGGLIRDILRNEVPIILQKE
                     VYVTASLIGGVIFVAANSVGLESDWVYLATVFTVIIIRLLAIRFNLNLPRL"
     gene            4463..5518
                     /gene="rsxD"
                     /locus_tag="PXB27_000366"
     CDS             4463..5518
                     /gene="rsxD"
                     /locus_tag="PXB27_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HDL5345196.1"
                     /translation="MFKMVSSPHTHSSNLTANFMLWVIAMMLPAFGVQLYYFGYGVLI
                     QTLLAVSLAIVIEIGIAKLRKKTTAFYLADLSGILTALILAMSIPPYAPYWLVLIGVI
                     VALLLAKHSYGGLGQNLFNPAMVGYALLLVSFPVQMTAWIVPIELLSEPPTFRDAISL
                     IFYGVTSDGFSIHQLVNSVDGIAQATPLDSMKSSLAKSGFDAVMNTPIFNGLFATGWL
                     QLNLAFLFGGLVLIYKRIIHWQIPVAMLATFAFFSGFTELFSQQSHLHFVSHFFSGAM
                     MFGAFFIATDPVTASITPRGKLIFGGLVGLLVYLIRYYGNYPDAIAFSVLLANICVPL
                     IDHYTQPRLYGTKLDNR"
     gene            5518..6150
                     /gene="rsxG"
                     /locus_tag="PXB27_000367"
     CDS             5518..6150
                     /gene="rsxG"
                     /locus_tag="PXB27_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HDL5345197.1"
                     /translation="MNTSKITLNYALILGFIALLCTAVSTGVYLLTKGKIDDVTAMQQ
                     RKLLEEVVPKSHFDNDVLATCKLVQLENAPYLNRIYVAKKSENLTAYLVQGTAPDGYS
                     GDIVLLMGIEPNGNILGVRTLEHKETPGLGDKIETRVSDWILSFTNQLFSLENEADWN
                     VKKDGGKFDQFTGATITPRAVVNHIRQSAKWVVTELAQNPTMFNQFKACK"
     gene            6159..6842
                     /locus_tag="PXB27_000368"
     CDS             6159..6842
                     /locus_tag="PXB27_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HDL5345198.1"
                     /translation="MEREQQIPVTNIDVAPKQSSVWKTIFADGVWKNNGALVQLLGLC
                     PLLAVSNSVTNALGLGLATMFVLICTNITVSLFRKITPHDIRIPIYVMVIATVVTAVQ
                     LLMNAFAFPVYQSLGIFIPLIVTNCIVIGRAEAYASKNEVKFSAFDGFSMGLGMTFSL
                     VVLGALREIIGNGTLFSGLDLLLGDWAKALRIDVLHIDSGLLLAILPPGAFIGLGVIL
                     AVKNIIDKR"
     gene            7283..8233
                     /gene="lpxM"
                     /locus_tag="PXB27_000369"
                     /gene_synonym="msbB"
     CDS             7283..8233
                     /gene="lpxM"
                     /locus_tag="PXB27_000369"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249087.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HDL5345199.1"
                     /translation="MTRITAQDGYTHKFLWSFLHPKYWGVWLGVLGLVILAYVPVRIR
                     DKLSAFVGKMAYRYLKQKNKKGYHRAKVNLRYCFPDWSEKKREEVVEKMFITVAQTML
                     AIGETAIRPASYLQKRCEFTGFENVVKAKESGKNVIMLVPHTWSIDMAGVAMFSLGYP
                     MTSMYNPHRNALVDWLWNITRERLGGRMHTRQNGIKAFLADVRKGDVGYFLPDEDFGE
                     ELSVYADFFATEKATLPGLNKMARVANAEVIPMFSIYNAEKAKYEMEILPPLEFSGDP
                     IQSAREMNRVIEYFVEKNPEQYVWILRLLKTRRNREDIYL"
     gene            8361..8900
                     /gene="apt"
                     /locus_tag="PXB27_000370"
     CDS             8361..8900
                     /gene="apt"
                     /locus_tag="PXB27_000370"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDL5345200.1"
                     /translation="MNQLDLIKSSIKSIPDYPKAGIIFRDITSLLEVPTAFQATIDAI
                     VAEFKDKGITKIVGTESRGFIFGAPVALALGVPFVLVRKPKKLPREVISQSYTLEYGE
                     DTLEIHKDAVSANDNVLVIDDLLATGGTIDATAKLIRRLGGNVEHAAFVIWLPELGGK
                     ERLEKEGVKSFSLVSFEGH"
     gene            8991..11048
                     /gene="dnaX"
                     /locus_tag="PXB27_000371"
     CDS             8991..11048
                     /gene="dnaX"
                     /locus_tag="PXB27_000371"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDL5345201.1"
                     /translation="MSYQVLARKWRPQRFSEVVGQQHVLSALENSLREDRLHHAYLFS
                     GTRGVGKTSIARLFAKGLNCVNGITAEPCGECANCKAIEEGHFIDLIEIDAASRTKVE
                     DTRELLDNVQYKPTVGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEYVKFLLATTDPQ
                     KLPITILSRCMQFHLRALEQSQIARHLEFILNAENIPFEPLALEKLAKAAQGSIRDSL
                     SLTDQAIAMSNANITLAIVSQMLGLIDDNQPLELVQATAQADGEKAMAIIEQIAAKGV
                     DWQQLLSDVAEVLHKIAMLQLVKTPTTEETLVHFLAKQIPPEDVQFFYQLILNSKKEL
                     PFAPEQRAGVEMAILRALAFHPKQAEKIATVAVEKVTQPNHSVSAVTTAKPQNQISQL
                     RENFEQNQQKTVTVANTTTAVESIAQPPQAVDTAISPSAPATLSPAMAALEARKKLQA
                     QQANLAQTEKKKPELKAPQVIAKKSANQTASLQERFISLAENQHKAVLQVSKKTESAP
                     KSVEDEDYRWTWLNPELENQADSAKPSDIKQAILQERTPELVAKTIHLSCQQDKWCDI
                     ANSLNLGGFSRQIALNSYLAEQNEQHLALVLKPNMAHLDNPESRKNLEAALAEKGYSY
                     ELTIGDNSEQQTPLEIRRAIFKQLTEAAKSALLNDEKLMLLRNAFDAQIDESTIRAVA
                     ETE"
     gene            11054..11962
                     /locus_tag="PXB27_000372"
     CDS             11054..11962
                     /locus_tag="PXB27_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5345202.1"
                     /translation="MKALELIDLVKTYPTGVKAVKGINLVVEQGDFYALLGHNGAGKS
                     TTIGIISSLVNKTSGSVKVFGYDLDTQKVELKHQIGLVPQEFNFNQFEKVIDVLVQQA
                     GFYGIPRSEALVRAEKWLKLLDLWEKRTHLTRELSGGMKRRVMIARALMHNPKLLILD
                     EPTAGVDIELRRSLWDFLRDLNNQGTTIILTTHYLEEAETLCRHIGIIQHGELIENTS
                     MKALLAKLESEVFVLDLQNSEQNQPLVIQNYPHKWLDENTLEVNVKRQQGLTNLFSQI
                     SAQGAEVLSMRNKSNRLEELFINMAQ"
     gene            11964..12725
                     /locus_tag="PXB27_000373"
     CDS             11964..12725
                     /locus_tag="PXB27_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5345203.1"
                     /translation="MNLIGFYTLAYKECRRILRIWRQTLVPPVITTTLYFLIFGTLIG
                     GRIGEMNGVGYTQFIAPGLIMMTAITASYVNTASSFFLSKFTRDFEEMLISPLSTHNI
                     IWGYVTGSILRAGLAATLVMCVAMVFVSFEIYSVAIIVLTLLLTIIAFALGGVINAVF
                     ARSFDDVGLIPTFVLTPLTYLGGVFYSISLLPEFWQTVSKFNPIVYMINGLRYGFLGI
                     SDVPLGYTFAVLTSLCIGLYIIAYSLIQRGVGLRS"
     gene            12746..13501
                     /gene="modA"
                     /locus_tag="PXB27_000374"
     CDS             12746..13501
                     /gene="modA"
                     /locus_tag="PXB27_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL5345204.1"
                     /translation="MKALKKLAITMALTLSGTAFSQEITVFAAASMTNVLQDIQQDFE
                     KAYPQDKITFSFASSSVLAKQIEQDAPADIFISADQKWMDYVAEKQPEKVKHRRNLVR
                     NELVLIAPTESNIEPKAIQAVDFAKELANSYLSVGDPNHVPAGKYAKKALSHYQLWES
                     VEPRLARAKNVRDALSFVERGESPLGIVYSTDAKVSNKVKVVAVFPPESYGEIVYPAA
                     AVSEKASAATFLDFLKTPKAKAKFEAAGFYPVN"
     gene            13685..14377
                     /gene="modB"
                     /locus_tag="PXB27_000375"
     CDS             13685..14377
                     /gene="modB"
                     /locus_tag="PXB27_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDL5345205.1"
                     /translation="MLNFTPQELNAIVLSLKVASIAVVLALPLAVWVAWLLARKQFWG
                     KNLLNGIMHLPLVLPPVVIGYLLLISMAKRGVIGQYLWEWFHFSLSFSWKGAVLASMV
                     MAFPLMVRSIRLAFEAVDPKLEQAARTLGAIPLKVFFTLTLPLSFSGIIAGGVLGFAR
                     SLGEFGATITFVSNIPNQTQTIPAALFTFIETPDGEMAAARLCLVAILISLIALFCSE
                     WLAERQKRYAQT"
     gene            14364..15419
                     /gene="modC"
                     /locus_tag="PXB27_000376"
     CDS             14364..15419
                     /gene="modC"
                     /locus_tag="PXB27_000376"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HDL5345206.1"
                     /translation="MLKLKLLQQLGNLTLNVNLDLPNKGITAIFGRSGAGKSSLINLI
                     AGLSTAQAGFIELNGQVLFDSQNGINLPPEKRNIGYVFQEHRLFPHYSVEKNLKYGYK
                     RSDSADFLQIVELLGIAPLLNRYPASLSGGEKQRVAIGRALLSSPNILLMDEPLSALD
                     LPRKQELLDYLDKLSARLEIPILYVSHSLEEVVRLADHLVLLENGKVTAFDTLSAVWH
                     SSAFSAWQPNHQRISLLELPVEETLPHYNMLGLSLNAQRLWVNQLPRHQIGEKLRVTI
                     ASKEVSISLQKPTQSSIRNVLKGNVRKIETHLDRVDLAIQVENQQIWSTISLWSFNEL
                     AIQPEQEVYLQIKSVSL"
     gene            15474..15908
                     /locus_tag="PXB27_000377"
     CDS             15474..15908
                     /locus_tag="PXB27_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HDL5345207.1"
                     /translation="MPIVNQSALVPYSAEQMYQLVNQYEKYPEFLSGCVSTKTLSTGE
                     NELKAELHIQKLGISQTFSTHNKMTPPYKIEMALIEGPFRHLHGAWTFTPFDEQSCKI
                     ALQLNFEFSSPIVAMVFGKVFNELTLKMVNAFKQRAKEVYGV"
     gene            15898..16233
                     /locus_tag="PXB27_000378"
     CDS             15898..16233
                     /locus_tag="PXB27_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HDL5345208.1"
                     /translation="MVSEQRQNPEEQKSEEQKINVEVLYAYPDKYFLKKLQVPQNATI
                     QTIILLSGVLEKYTEIDLRENKVGIFSRPAKLNETVEEGDRIEIYRPLIADPKEIRRK
                     RAAEQAEKK"
     gene            16437..17726
                     /locus_tag="PXB27_000379"
     CDS             16437..17726
                     /locus_tag="PXB27_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5345209.1"
                     /translation="MDIRQKIAHNKMTSFQWLVVGLTVLLNMLDGFDLMALAFTAKSI
                     REELQLDGAAIGSLMSAGLFGMAIGSLFLSPLADKFGRRPLLLIGTGLATLGMLLTYF
                     ATSAFEIGVWRVLTGLGVAAILTGTNVLVSEYSSQKWRGLAIAIYASGFGIGAMLGGM
                     SAVLLQGEYGWRAVFLVGSILTGAVFVLLFFVLPESVEFLISKHPVNAKARLNQIAVK
                     LGLGNGWELPEIEAKSKSKVAITRLFEPEYRRNTLLIWLAFIAISASFYFISSWTPAL
                     LEEAGMPKTQSQTVGMAISVGGTIGSLIFGFLVSRWAAKHILQAFSILAASAVVAFVF
                     AGNLGLAITLAILVGGLINGCITGLYTINPTLYAPDFRSTGVGTAIGVGRLGSIVSPT
                     LAGILLDAGWKKNELYLGAAVVLLIAAFTVHFLKQRK"
     gene            complement(17801..19255)
                     /locus_tag="PXB27_000380"
     CDS             complement(17801..19255)
                     /locus_tag="PXB27_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HDL5345210.1"
                     /translation="MSEISTLAPQLLWQWFDKACAIPHPSYHENELAEFIVNWAKSKN
                     FFVERDEVGNVLIRKPATKGMENRQTVALQAHLDMVPQANTGTVHDFTKDPIQPYIDG
                     EWVKAKGTTLGADNGIGLASCLAVLDSDDLAHPTIEVLLTMTEEAGMEGAIGLRPNWL
                     TADIMINTDTEENGEIYIGCAGGENVNVTLPIFLVPHQQDSAVTVTLKGLNGGHSGCD
                     IHTTRANAIKVFARILEQAHNEVEFQIAEIQGGSVRNAIPREAHATLVFSAKNQQKLT
                     ACLSQTAEQVKAELKIAEPNFQFLIEEAQLPEQAFDLASTARVIHFLNVMPNGVVRNS
                     DVVENVVETSLSIGVLNTEASEVKATILARSLIEGGKEEIRSKIRSLTALVGAEVEFS
                     GNYPGWEPNPNSKITPLTKAIYDEILGYESQIKVIHAGLECGLINKIYPNMDFVSIGP
                     TILNAHSPDEKVHIPAVEIYWKLLTKLLEQAPEK"
     gene            19455..20539
                     /locus_tag="PXB27_000381"
                     /pseudo
     CDS             19455..20539
                     /locus_tag="PXB27_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
     gene            20735..22144
                     /locus_tag="PXB27_000382"
     CDS             20735..22144
                     /locus_tag="PXB27_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HDL5345211.1"
                     /translation="MKKYPLLFSLFSPVVWAEKVIEHNTDPISFERQPQAVKFEQNVV
                     KNSQKPTAYQPAYSVNQNVEQQINQAILSQDWATLEPLLSRYPQTENFDPLLWHYAQA
                     ALAYAKKDHQTAVFHYRQLLAKPPSLVYPRFDLALILAEDQQFRAAEQEFKAIQAHLP
                     PELAKIARNTQVQIAENEAWQPDFYLQYTQTDNVNNASSSPIVNVNGRIFRKNADSLP
                     QSAKGVRYGLGLSKLHNVKGNHFVGLTLNYSGIYYWDNRDYREQSVSVSPNYSYRTVA
                     YRLTFSPFIEQNWLGSSRYNVQFGATLNGLRYLNSQWLVSGSFTHLQKRYFEELTASR
                     YNSYQHQLSGGVQWQAVKNGRFFANLDGVREIAREKAQSSNKWLARVGAVFQGERWAT
                     QASIGFGKRYFADKHYLFGYKRLDTEYQANLAVWNRTWHWQGIVPKLNFRYQKIDSNI
                     ADFYSRQNKEVFFTLEKLF"
     gene            22290..22763
                     /gene="gpt"
                     /locus_tag="PXB27_000383"
     CDS             22290..22763
                     /gene="gpt"
                     /locus_tag="PXB27_000383"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDL5345212.1"
                     /translation="MSEKYTNEKYVVTWDMFHMHARKLAENLLPATQWKGIIAVSRGG
                     LFPAAVLARELGIRHVETVCIASYDHDSQGELNVLHAADTDGEGFIVVDDLVDTGNTA
                     KEIRRMYPKARFVTVFAKPHGAPLVDDYVIDIPQETWIEQPWDLGITFVQPLARR"
     gene            22876..23499
                     /gene="gmk"
                     /locus_tag="PXB27_000384"
     CDS             22876..23499
                     /gene="gmk"
                     /locus_tag="PXB27_000384"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDL5345213.1"
                     /translation="MSLGNLYIISAPSGAGKSSLINALLADLPRSEVQLSISHTTRQP
                     RPAEQDGVHYYFTSHDEFKQLIEQGYFLEWAEVFGNYYGTSLPMIERSLEQGIDVFLD
                     IDWQGARQIREKVPNVKTVFILPPSKTELEKRLIGRGQDSAETIAKRMAEAVSEMNHY
                     NEFDYVIINDDFQQALAELKAILIAERLSLGSQQIRHQHLITDLLNN"
     gene            23589..24986
                     /locus_tag="PXB27_000385"
     CDS             23589..24986
                     /locus_tag="PXB27_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HDL5345214.1"
                     /translation="MKLSKLAFSVALALTVTACTTGNMSNDGTLQQAQQTYQSYDEIT
                     KQYQINEQWWQGYTDAELNRVVDLAIKNNLDLAKSGIAVNKALYNANLVGANLVPTFS
                     GSGSVSAAKGLGSASSNNVSTGVSTTAAQAAFNLSYTVDLWRRLADTASAAEWTHKAT
                     IEDLKATRLSIINAVVTTYYQLAYYRDAIRITEESIRHYEQISRILTNKMNAGAIDSL
                     AVDQAQQAVLNARNSVIDLKTNQKIAEQTLRNLLNVQPNTPLNIRYPNIMSVKLQGVD
                     LNVPVSAIANRPDVVGSLQRLQSAFKTLTATEKSWFPTLTIGGSITGSATNIGNVADN
                     PVGNGLIKLDLPFLDWNRVKNNVKISETDYVTARLNYEQKITSALNEIDRYYYSYTQS
                     RSSLKILQDTNAKNKRISSYYKNRYEQGVSEFREWITALNTELNSQLAILNAKYAILT
                     NENAVYQAMAGKYRR"
     gene            complement(25099..25944)
                     /locus_tag="PXB27_000386"
     CDS             complement(25099..25944)
                     /locus_tag="PXB27_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL5345215.1"
                     /translation="MTFVKKTALSLIATALFATSSFAAEQSQYQHIRNATAKIEYAGK
                     TFLVDPYLAEKDRYLPPNAKKRNPTLDMTVSIDEVLNGVDAVIVTHTHHAHWDDVAQQ
                     RVPKNLPIFVQNAGDARLIRSQGFTDVRVVGKDTPFEGVTLTRTRGQHGTDAMYANPK
                     IAEMVGDAMDVVMKAEGKPTVYLVGDTIWNHEIEHSFNTYKPDFVVLNAGCAKLPNFE
                     GSPIMCTEDVSKAHKIAPQAKILTVHMDALPQTSISSDQMRDYVQKEGLNRVTVPKNG
                     DVIKF"
     gene            complement(26085..29180)
                     /gene="ttrA"
                     /locus_tag="PXB27_000387"
     CDS             complement(26085..29180)
                     /gene="ttrA"
                     /locus_tag="PXB27_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetrathionate reductase subunit TtrA"
                     /protein_id="HDL5345216.1"
                     /translation="MNNKRRNLLKGGLALGGAAAFGAGYSPKVSEIAKGVMHGTSGEK
                     TRDAIHGNSLTPEYQVKDGKLLSNPEQIVCNTQCMGCWTQCGIRARVDLNTNQVIRIS
                     GNPYHPLSSDKTLPFGMPIAKAELLLAGESGIENRSTACARGAAFLEGVNSPYRITQP
                     LKRVGKRGEGKWQTISFEQLVEEIINGGNLFGEGEVEGLKTIRNLVDPINPKQPDLGM
                     KANQLLVTFAGPEGRQPILQRFAQKSFGTINFSNHGAYCGAAYRAGSGAFMKDFDNQQ
                     HAKPDWDHAEFILFIGTSPAQSGNPFKRQARQLAKRRPDDNFSYVVVSPRLEMTSSLA
                     TQNNHWVPIKPEGDLALVMGMLRWIIENERFNADYLSRPSLAAMENAGHVSYANASHL
                     IICEPNHPKFGQALRISDLQDVQLDPEEKVEENLIVVKDQASGELISAKDCLQAVIFV
                     EQIAKLLDGSEVLVKSSMQLLKEATQEKTLAEYSDICGVPVQTIENLAKKFTSHGHKA
                     NAITHGGTMHSTGFYTSWAILLLNAMVGNLNKKGGMAVAAGKFKDFGDGPRYNLANFP
                     NEVKPKGTNLARSKKAYQNSSEFKQKVTKGENPYPAQGAWYPFTGGQTTEMFTSMVQG
                     YPYFAKAWINHMANPVYGLTAMNPIALEKLKDPKILPLFVSIDAFMNETTALTDYIVP
                     DTHNFESWGFSTAWGGVPQRTSTARHPIVPSKNAKTAEGDTICMENFVIAVAKKMGLP
                     GFGENAIADLEGNRYPLHRTEDYFLRAAANIAFAGEKPAPDASEQDLLLTGVQRIMPK
                     IEQTLKAEERLKVANVYCKGGRFAPHESAWKEENMEFQWKNCLQIWNENVYKAQHHSN
                     GEHYWGCPRYMSPRFADGSTLEQHYPQSEWGFKLISFKSNIMSSITAPLLRLLSIKPE
                     GLVAMNRLDAEEKGIKHGDVVKLSTPTAGLTVQIVVLDGIARGTIGIEHGYGHKQIGA
                     SSYTIDGVEIPANPMIAKGINLNDLGIIDHTKAVKSPWVDWVCGSAVRNGIPAKVEKL
                     A"
     gene            complement(29170..30201)
                     /gene="nrfD"
                     /locus_tag="PXB27_000388"
     CDS             complement(29170..30201)
                     /gene="nrfD"
                     /locus_tag="PXB27_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HDL5345217.1"
                     /translation="MIREVLVEPQHIVWLPWIVHYFFFVGVAATAVFTAVLFAKKSLK
                     PTACELAAVTVALIGSIVAPVALTADLHQPSRILHFYTDFAWRSPMAWGAIILPLFSV
                     AVAGYFVLALAHHTRPNLPKWLAWLQLPILQNQGLLWAFRLFATLTAIGIIGYTVLET
                     YQTGTRTLWHSAWLLPIMLFSAWAVALGLTQAVCKMLFHTQTNTGLWTKLCLILTALS
                     LVGLAFSSPQATQDFAQLFNGSITAYLVGIFWLIALICNFSAKNHRLQWLGVLALIAF
                     GWLLRWVLVIQVQTIAKTNALQNPYHFDWTAVDGGLGIVSIFGLTVLVSIVVWQLILI
                     ICGGRKGEQ"
     gene            complement(30203..30931)
                     /gene="ttrB"
                     /locus_tag="PXB27_000389"
     CDS             complement(30203..30931)
                     /gene="ttrB"
                     /locus_tag="PXB27_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetrathionate reductase subunit TtrB"
                     /protein_id="HDL5345218.1"
                     /translation="MDLSKRSFLKELSALTVGASFVPLQSAQAFMPAWREGDENKRYA
                     MLIDLRKCIGCQACTVSCSVENATPLHSFRTTVRQYEITNGTQVVNNVVLPRLCNHCD
                     QPPCVPVCPVQATFQRKDGVVVINNEQCIGYGYCVQACPYDARFINEETKTADKCTFC
                     THRLEVGLLPACVESCVGGARVIGDLNDPKSQISQLYQAHKDDLKVLKPEAGTVPHVF
                     YVGLDDDAFVSKIDGQPMLWTGEA"
BASE COUNT         8608 a         6749 c         7192 g         8673 t
ORIGIN      
        1 tctatatcaa aggagtgaga ctttttagga ttaacttaga aaatcaagct cctttcacct
       61 ttcatgctgt atagcttagt ccccacattc tccattctac cagagataga aacaaaggta
      121 acaagatcat atttgatatg gtatgtttac tagaagtacc tccgatttct tattgtgaat
      181 gagccgaaaa caagagataa tataacgttt atttttaatg agatgcttta acaaacggtc
      241 agattttctc tgttttttgc aaaataaaat gcgtttttga ccgcttgtat atttaatttt
      301 gtggaattta tggtcgatta tattctgttg gtcatcagca ccgcccttat taataacttt
      361 gtcttagtta aatttctagg gctttgtccg tttatgggag tgtcgaaaaa ggtggaaact
      421 gctattggta tggggttggc gacaaccttt gtgctgacgg ttgcctcgct ctctgcttat
      481 ttgatggaaa cctatttgct tgagccgtta aacgctcaat ttttgcgtac cttggtgttt
      541 attttagtga ttgcggtgat cgtgcaatta acggaaatga ttgtgcataa aaccagcccg
      601 acactttatc gcttgcttgg gatctattta ccgctgatta ccaccaactg tgccgtatta
      661 ggggtggcat tattaaacgt gaatttagcc aataacttgg tggaatctgt tctttatggt
      721 tttggagccg ctgccggttt ctcgctggtg ttagttttat ttgcggctct gcgtgagcgt
      781 ttggctgccg ctgatgtgcc tcgtccgttc caaggagcat caattgctct gattaccgca
      841 ggcttgatgt cgcttgcctt tatgggcttt acaggattgg tgaaactcta atgcttgatt
      901 tgccaattat cgcttatatt cttatcgcta ttgctgtgat cgcactgatt ttcggggcgg
      961 ttttaggcta ttcctcgatt aaactgaaag ttgaatccga cccgattgtc gatcaaattg
     1021 atgccctttt accacaaagc caatgcggtc aatgtggcta cccgggctgt aagccttatg
     1081 ctgaggcgat tgctaatggt gatgtgatca ctaaatgtgt gccgggtgga caacctttag
     1141 tggtgaatct tgccgaattg cttggggtag aagtgccgcc aatggaaggt atggcagagc
     1201 ctgagatgaa agtggctttt gtgattgaag atctctgcat tggctgtact aagtgcattc
     1261 aggcttgccc ggttgatgcg attatcggca cgaataaagc aatgcacacc attattccgg
     1321 atctttgcac cggctgtgaa ctttgtgtcg ccccttgccc gaccaactgt attgaaatgc
     1381 gaccaattaa accgacaacc caatcttgga actggaaatt tgaccaagat ttaattattc
     1441 cgattgtgaa taccactgaa ttgcagagaa aattagttac cggcggaggc aaaaatgagc
     1501 aatcctaatt tagtgattga gcgaatccgc caaaataaag aaacgggtaa actgtgggat
     1561 ttcccgggcg gtattcaccc gatggaaaat aaatcccaat caaacagcaa gccaattcgc
     1621 accttaaatt taccgaagtt tttctatgtg ccactggttc aacattctgg ctgggcaggg
     1681 gaattattag tcaaagtcgg cgatgtggta ttgaaagggc agccgctcac gaaaggcgat
     1741 cacttccgtc aactgcccgt acacagccca acctccgggc gggtgattgg gattgagcct
     1801 tatgtttccg ctcacgcctc aggtttgccc gaaataacgg tgattattga aaccgatggt
     1861 aaagataaat gggtggaacg ccagccgatt gaggattttt taagcctaac aagcgatcaa
     1921 attatccaaa aagtttacca atatggcatt gccggtttag gcggtgcggt attcccaacc
     1981 gcaacaaaat taagtttggc ggataaacgc tgtaaattac tggtgattaa cggggcagaa
     2041 tgtgagcctt acatcacctg tgacgatcgc ttaatgcaag atttcactgc ggatcttatc
     2101 gaaggtattc gcattctgcg ttatgtgctt cgcccggaag aagtggtgat tgcgattgaa
     2161 gacaacaaac cgcacgctat tcaagcttta gaaaccgctt taaaaggctc gaacgatatg
     2221 tttgtgcggg cgatcccgac taaatatccc tccggagctt ctgatcagct gattcaagtg
     2281 ttgaccggac ttgagattcc ggctggcaaa cgcactattg aaatgggcat tgtgatgcac
     2341 aatgtcggca cggcgtttgc ggttaaacgg gcaattattg atgatgaacc tttgattgaa
     2401 cgggtagtga cgctgacagg cgataaaatt cgcaacaagg ggaacgtgtg ggcaagatta
     2461 ggcacgccaa ttcagcactt gttggagcag gtagattatc aagccgataa acgctttccg
     2521 gtgtttttag gcggaccgat gatgggcttt attttgcctg cattgcaagc cccgattacc
     2581 aaaactgcga actgcttgat tgcccctgat cattttgaat atgcaccacc tgaaccggag
     2641 cgtagttgta tccgctgttc aagctgttcc gatgcttgcc cggtggggtt attgccacag
     2701 caactttatt ggtttgcccg tgcagaagat cacgataaat cgaaagaata ccatttagat
     2761 gcctgcattg agtgtggcgt ttgtgcttac gtttgcccaa gctacattcc gttaatccaa
     2821 tatttccgcc aagaaaaagc aaaaatcgag gaaattgagg aaaaagcccg caaagcggaa
     2881 gaggctaaaa tccgctttga ggcgagagaa gctcgcttac aaaaagaacg agatgccaga
     2941 actgctcgca ttagccaagc ggcggaaaaa cgccgtgaag aagtggcaaa tgcaggtggt
     3001 gttgatcctg taaaagcggc tcttgaacgc ctgaaagcga aaaaagcggt tgaaacagtc
     3061 caagaaatac cggcagaagt acaggtaaaa cctaacggcg agcctgataa cagcgattta
     3121 atggcacaac gcagagccag acgtttagcc aaacaggcag aacaagcggt cgaaaaatcg
     3181 gaaaatgtta caaatgcctt gattggggca aataatgatt tgcctctaca atcaatgaac
     3241 gaaactgcat cggataaatc tgtaggggcg aataactatt cgcccgaagc agaaagcgaa
     3301 gacccacgca aagccgccgt tgcggctgcc cttgcccgag ccaaagccaa aaaacaggca
     3361 cagcaagcgg tcgaaaaatc agaaaatatt accaatgccg tgattggggt aaataatgat
     3421 ttgcccctac aatcaatgaa cgaaacggta tcggataaat ctgtaggggc gaataaccat
     3481 tcgcccgaaa cagaaagcga agacccacgc aaagccgccg ttgcagcagc tatcgctaga
     3541 gctaaagcga aaaaacaagc tcaacaagcg gtcgaaaaac cggaagatat tgccaatgct
     3601 gtgattgggg taaataatga tttgccccta caatcaatga acgaaactac atcggataaa
     3661 tctgtagggg cgaataacca ttcgcccaaa acggaaagcg aagacccacg caaagccgcc
     3721 gttgcggccg ccatcgccag agctaaagcg aaaaaagccg ctcagcagga gaaaagcaac
     3781 aatgtttagc gagctaattc cggcattgcc gtggtcgttc ctgtttattc aggacttaat
     3841 cggcactatc gtgtttgcga tttcgggggc aattgctgcc cgccagcaca aaatggacat
     3901 tttcggtatg tttattttag cgtttgtgac cggggtcggg ggcggtacgc taagggatat
     3961 gatgattggc tccacgccgg tgttttggat gaaacaaccg atctatgtgc tgatgattac
     4021 ccttgcagtg gtgattaccg ctattttccg caaccaaatt tcccgcaaaa gttggcaaac
     4081 cggcttgctg gtgtttgatg ccatcgggct tggcgtattt accgtgattg gcgtgcaaaa
     4141 aggcttggat ttcggcttgc acccactgat ctgtgtggct ctgggcggtg tgacaggctg
     4201 ttttggtggc ttaatccgtg atattttacg caacgaagtg ccgatcattt tgcaaaaaga
     4261 agtgtatgtg accgccagcc ttatcggtgg tgtgattttt gtggctgcaa actcagttgg
     4321 gctcgaaagc gattgggttt accttgccac cgtgttcacc gtaattatta tccgcctgct
     4381 ggcaatacgc tttaatttga atttgccgag actttaataa gcggtcattt ttttgcaatt
     4441 ttttactaaa aaggttttat agatgtttaa aatggtcagt tccccccaca ctcattcgag
     4501 caatctcact gcgaatttta tgctgtgggt gattgcgatg atgttacctg cttttggggt
     4561 tcagctttat tatttcggct atggtgtgct gattcaaacg ttgcttgccg ttagccttgc
     4621 gattgtgatt gaaatcggga ttgcaaaatt acgcaaaaaa acaaccgctt tctatttggc
     4681 tgacttatca ggtattttaa ccgccttgat tttggcgatg tcgattccgc cttatgctcc
     4741 ttattggctt gtgttgattg gcgtgattgt ggcgttatta ttggcaaaac acagttatgg
     4801 tggcttgggg cagaatctat ttaacccggc aatggttggc tatgcgttgt tgttggtttc
     4861 tttcccggtg caaatgacgg cgtggatagt gccgattgag ttattaagtg aaccaccgac
     4921 atttcgtgat gcgatttcgt tgatttttta cggggtaact agcgatggtt ttagtattca
     4981 tcagctagtt aatagtgttg atggcattgc acaagctacc ccgcttgata gtatgaaaag
     5041 cagccttgca aaatcaggtt ttgacgcggt aatgaatacg ccgattttca acgggctgtt
     5101 tgcgaccggt tggctacaac tgaaccttgc gttcttattc ggcggtttgg tgttgattta
     5161 taaacggatt atccattggc aaattccggt agcaatgttg gcaacttttg ccttttttag
     5221 tggattcacc gagctgttta gccaacaatc gcacttgcat tttgtgagcc atttctttag
     5281 cggtgcgatg atgttcgggg cattttttat tgcgaccgac ccggtaacag cctccattac
     5341 cccaagagga aaactgattt tcggtggctt ggtgggatta ttggtttatc taatccgcta
     5401 ctacggaaat taccctgatg cgattgcatt ttcagtgtta ttggcaaaca tttgcgtacc
     5461 gttaatcgac cattatacgc agccgagatt atacggcaca aaattggata acagataatg
     5521 aacacctcaa aaattacctt aaattacgcc ttaattttag gctttattgc cttgctttgc
     5581 accgctgttt caaccggtgt gtacctgctt accaaaggca agattgatga tgtaaccgca
     5641 atgcaacaac gcaaattatt ggaagaggtt gtgccgaaaa gccattttga taatgatgtg
     5701 ttggcgactt gcaaactggt tcagctcgaa aatgcccctt atttgaaccg tatttatgtt
     5761 gcaaaaaaat cggaaaatct gaccgcttac ctagttcaag gaacggctcc ggacggctat
     5821 tccggcgata ttgtgctatt gatggggatt gagccgaacg gcaacatttt aggcgttcgc
     5881 acccttgagc ataaagaaac tccggggctg ggagataaaa ttgagacccg agtgtcggat
     5941 tggatcttat cttttaccaa tcaacttttt agtttagaaa acgaagcgga ttggaatgta
     6001 aaaaaagatg gcggcaagtt cgaccaattt accggtgcaa ctattacacc tcgtgcggtg
     6061 gtcaatcata tccgccaaag tgcgaaatgg gtggtgacag agctggctca aaacccgaca
     6121 atgtttaatc aatttaaagc gtgcaaatag ggggagaaat ggaaagagaa caacaaatcc
     6181 ctgtgacgaa tattgacgtt gccccgaaac aatccagcgt ttggaaaacg attttcgctg
     6241 atggtgtgtg gaaaaataat ggtgctttgg tgcaattatt agggctttgc ccactactgg
     6301 cggtttcaaa cagcgtaacc aatgcacttg gactaggctt ggcaacaatg tttgtgctaa
     6361 tttgcaccaa tatcacggtt tccctgtttc ggaaaatcac tccacacgat attcggattc
     6421 cgatttatgt gatggtaatt gcaacggtgg taacggcagt acagctttta atgaatgctt
     6481 ttgctttccc tgtttaccaa tcgctcggga tttttattcc gctgattgta accaactgta
     6541 tcgtgattgg acgagcagaa gcctatgcct caaaaaatga ggtcaaattc tcggcttttg
     6601 acggcttttc aatggggctg ggaatgactt ttagccttgt ggtgctaggc gcgttgcgtg
     6661 aaattattgg caacggcacg ctgttttcag gcttagatct actccttggc gattgggcaa
     6721 aagccctacg cattgatgta ttgcatattg attcaggttt actactggca attttacctc
     6781 cgggggcttt tattggactt ggggtgatat tggcggtgaa gaatattatt gataagagat
     6841 gatgtgttat gtcacttttc tttgcttgtg caaagaaaag taaccaaaag aaagcacacc
     6901 ctgctttgtt gctatcctca cttggttgaa attttcttaa cgaaaaataa ccctgatgtt
     6961 cgcttcgctc tcgctcgagt tattttccta aaaatttcaa gtcgttcggg caactcagaa
     7021 gggaacccga tccgaccata caagcggtga tttttttgga aaattttgca aaatgatgct
     7081 ctgccgggtt cccttctgaa ccgcccgaac gactttgaat ttgtaggaaa aattccttgt
     7141 ttgagcagcg aagcggcgag tttggaattt tttcgttaag caaattcaaa ccaagcgaag
     7201 atagcggtga agtagggttg cctttctttg gttactttct ttggcgaagc aaagaaagtg
     7261 acaaataatg aggaattata gaatgacacg aattaccgcc caagacggct atacgcacaa
     7321 attcctatgg tcttttctac acccgaaata ttggggcgta tggttaggtg tattaggatt
     7381 agtgatttta gcctatgtgc cggtgcgaat tagggataaa ttatccgctt ttgtgggtaa
     7441 gatggcgtat cgctatctca agcagaaaaa taaaaaaggc taccaccgtg ctaaagtgaa
     7501 tttgcgttac tgttttcccg attggagtga gaaaaagcgt gaagaagtgg tcgagaaaat
     7561 gtttatcacg gtagcacaaa ccatgttggc gattggcgaa actgcaatcc gcccggcctc
     7621 ttatttacaa aaacggtgtg aatttaccgg ctttgaaaat gtggtgaaag ccaaagagag
     7681 cggtaaaaac gtgattatgt tagtgccaca tacttggtcg attgatatgg cgggggtggc
     7741 gatgttctcg ctcggttatc cgatgacatc aatgtacaat ccacaccgta atgcgttggt
     7801 ggactggctg tggaatatta cccgtgagcg tttaggtggg cgaatgcaca ctcgtcaaaa
     7861 tgggattaaa gcgtttttag ccgatgtgcg taaaggtgat gttggttatt ttctgcccga
     7921 tgaagatttc ggcgaagagc taagtgtgta tgccgacttt tttgccaccg aaaaagccac
     7981 tttgccgggc ttaaataaaa tggcaagagt ggcaaatgcg gaagtgatcc caatgttctc
     8041 gatttataat gcggaaaaag caaaatatga aatggaaatt ttgccaccgc ttgaattttc
     8101 aggagaccca attcaatccg cccgagaaat gaaccgagta attgaatatt ttgtggagaa
     8161 aaaccccgag cagtatgttt ggattttacg tttgcttaaa accagacgaa acagggaaga
     8221 tatttatcta taatataggg aaaaggaaac tgaaaagtaa gctgtactat caaacgtaag
     8281 gttatcaatt tctccttgaa atttctgctg tttttctata gagttttcca gttatttttt
     8341 tattacacgt aggtcaaaca atgaaccaat tagatttaat taaatcttct atcaaatcca
     8401 ttccagatta tccgaaagca gggattatct tccgtgatat cacttcactt ttagaagtgc
     8461 caactgcatt ccaagccacc attgatgcca tcgttgcaga atttaaagac aaaggtatta
     8521 ctaaaattgt cggcacagaa tctcgcgggt ttatttttgg tgcccctgtt gcattagcgt
     8581 taggcgtgcc ttttgtgttg gttcgtaaac cgaaaaaatt accacgtgaa gtgatttcgc
     8641 aatcttacac gcttgaatat ggtgaagaca cgctagaaat tcataaagac gcagtgagtg
     8701 ctaatgataa tgtattggtc atcgacgatt tactcgcaac cggtggtact attgatgcaa
     8761 ctgcaaaact aattcgtcgc ttaggtggta acgtagaaca cgctgccttt gtgatttggc
     8821 tgcctgagtt aggtggtaaa gagcgtttgg aaaaagaagg ggttaaatcc tttagcctag
     8881 tcagctttga aggtcattaa tggggaacaa gcggtggctt tttgctaaaa ttttgcaaaa
     8941 tttttcggaa agatgaccgc ttgtgatttc caaattgaat agaaaaatca atgagttatc
     9001 aagttttagc ccgtaaatgg agaccgcaac gcttctcgga agtagttggt cagcaacacg
     9061 ttttatctgc tcttgaaaac agcttaagag aagatagact gcaccacgct tacctgtttt
     9121 caggcactcg tggtgtgggt aaaacctcta ttgcccgttt attcgccaaa ggcttaaact
     9181 gtgtaaacgg gatcaccgcc gagccttgcg gtgaatgtgc gaattgtaaa gcgattgaag
     9241 aagggcattt tatcgatctc attgaaattg atgctgcttc tcgtactaaa gtggaagaca
     9301 cccgtgagct acttgataac gttcaataca aacccaccgt tgggcgtttt aaggtttatt
     9361 taattgacga agtgcatatg ctctctcgtc atagtttcaa cgccttacta aaaacccttg
     9421 aagagccgcc tgaatatgtc aaattcttgc tggctaccac tgatccgcaa aaactgccga
     9481 ttacgatcct ctctcgctgt atgcaatttc atctgcgggc attagagcaa tcgcaaattg
     9541 cccgtcatct tgaatttatt ctaaatgcag agaacattcc gtttgagcct ttggcactag
     9601 aaaagttggc aaaagcagca caaggtagta tccgagactc tctcagcctc accgatcaag
     9661 ccattgcaat gagtaatgcc aatattacgc ttgcgatagt cagccaaatg ctagggttga
     9721 ttgacgataa tcaaccacta gaattagtgc aagcaaccgc ccaagccgac ggtgaaaaag
     9781 caatggcgat catcgagcaa attgcagcga aaggcgttga ttggcaacaa ttattaagcg
     9841 atgtggcaga agtgttacac aaaattgcaa tgttgcaatt agtgaaaaca cctacaaccg
     9901 aagaaacatt agtgcatttt ctggcaaaac aaattccgcc cgaagacgtg caatttttct
     9961 atcaattaat cctcaacagt aaaaaagagt tgccttttgc accggagcaa cgtgccggtg
    10021 ttgaaatggc gattttgcgt gcgttggctt tccaccctaa acaggcagaa aaaatagcaa
    10081 cagttgcagt ggaaaaggtt acacaaccaa accattcggt ttcagcggtt acgacagcaa
    10141 aaccacaaaa tcaaattagc cagctaagag aaaattttga gcaaaatcag caaaaaacgg
    10201 ttactgttgc caatactacc acagcggttg aaagcattgc tcaaccgcca caggcagtag
    10261 ataccgctat ttcgccgtct gctcctgcga ctctttcacc ggcaatggca gccctcgaag
    10321 cccgcaaaaa gctacaagca cagcaggcaa atttagctca aaccgaaaaa aaaaagcctg
    10381 agctaaaagc ccctcaagtt attgcaaaaa aatcggcaaa tcagaccgct tcattgcaag
    10441 agcggtttat cagcttggcg gaaaatcagc acaaagccgt actgcaagtt tcaaagaaaa
    10501 ccgaatccgc ccctaaaagt gtggaagatg aagattatcg ctggacgtgg ctcaatcctg
    10561 aattagaaaa tcaagcagac agtgccaaac cttctgatat taaacaagcg attttgcaag
    10621 aacgcacccc tgaattagtg gcaaaaacca ttcatttatc ttgtcagcag gataaatggt
    10681 gtgacatcgc caactcactc aatttaggcg gattttcccg tcagatcgcc ttaaatagct
    10741 atttagcgga acaaaatgaa cagcacctcg cattggtgtt aaagccgaat atggctcact
    10801 tagataatcc ggaatcacgt aaaaatttag aagctgccct tgccgaaaaa ggctattcat
    10861 acgaattgac cattggtgac aatagcgaac agcaaactcc gcttgaaatt cggcgagcca
    10921 ttttcaaaca attaactgag gcagcaaaat cagctctgct taacgatgaa aaattaatgt
    10981 tacttcgcaa cgcttttgat gcacaaattg atgaatcgac cattcgagcg gtggcggaaa
    11041 ctgagtaaaa caaatgaaag cattagaatt aattgatttg gtaaaaacct acccaacagg
    11101 tgtaaaagcc gttaaaggca ttaatcttgt ggtcgagcaa ggtgatttct atgccctact
    11161 tgggcataac ggggcaggta aatctactac tattggcatt atcagctcac tggtaaataa
    11221 aaccagcggc agcgtgaaag tgtttggcta tgatttagac acccaaaaag tcgaattaaa
    11281 gcatcaaatc ggcttagtgc cacaagagtt taatttcaat cagtttgaaa aagtgattga
    11341 tgtgctagtg caacaagccg ggttttacgg cattcctcgc agtgaagcct tagtacgtgc
    11401 cgaaaagtgg ctgaaattgc tcgacctatg ggaaaaacgc acccacctca cccgtgagct
    11461 ttctggtgga atgaaacgtc gggtgatgat tgcccgagcc ttaatgcaca atccaaaact
    11521 actgatttta gatgaaccta ccgcaggggt ggatattgaa ctccgccgct cattgtggga
    11581 tttcttgcgg gatctaaaca atcaaggcac aactattatt ctcactaccc actatttaga
    11641 agaggcggaa accctatgcc gtcacatcgg cattattcaa cacggcgaat taattgaaaa
    11701 cacctcaatg aaagccttgc ttgccaaatt ggaaagtgag gtatttgtgt tagatttgca
    11761 aaattctgag caaaatcaac cgcttgtgat tcaaaattac ccgcataaat ggcttgatga
    11821 aaacaccctt gaagtgaatg taaaacgcca gcaagggcta accaatctgt tttcgcaaat
    11881 ttccgcccaa ggggctgagg tgctaagtat gcgaaacaaa tcaaaccgct tggaagaact
    11941 atttatcaat atggctcagt aaaatgaatt taatcggatt ttatacctta gcttacaaag
    12001 agtgccgccg cattttgcgg atttggcgac aaactttagt gccaccggtg attactacca
    12061 cgctctattt tttaatcttc ggcacgctga ttggtgggcg aatcggggaa atgaatggcg
    12121 tgggttacac ccaatttatt gcacccggct taattatgat gaccgccatc accgcctctt
    12181 atgtgaatac cgcctcttca tttttcttga gtaaatttac ccgagatttt gaagagatgc
    12241 tgatttcgcc actctctacc cataatatta tttggggtta tgtcaccggc agtattttgc
    12301 gtgcaggttt agccgcaacc ttagtaatgt gcgtggcaat ggtgtttgtc tcgtttgaaa
    12361 tctactcggt tgcgattatc gtcttaaccc tgttgctgac aatcattgcc tttgcgttag
    12421 gtggcgtaat taatgcggtg ttcgcccgct cctttgatga tgtggggctg attccaacct
    12481 ttgtgcttac tcccttaact tacttaggcg gcgtatttta ctctatttca ttactgcctg
    12541 aattttggca aacggtgtct aaattcaacc cgatcgttta tatgattaac ggcttaagat
    12601 atggctttct cggtatcagc gatgtgccgt taggttatac ttttgcagtg cttacttcac
    12661 tttgcatcgg cttgtatatt attgcctatt ccttaattca acgaggggtt gggctgcgtt
    12721 cttaactaat tgtgaggtta tgaaaatgaa agcattaaaa aaactagcaa ttacaatggc
    12781 attaacgtta tcaggcacag ccttttcaca agagattacc gtgtttgctg ccgcttcaat
    12841 gacaaatgtg ttacaagata ttcagcagga ttttgaaaaa gcctatccac aggataaaat
    12901 cacgttctcc ttcgcttcat catcggtatt agcaaagcag attgagcaag atgcaccggc
    12961 agatattttt atttccgccg accaaaagtg gatggattat gttgccgaaa aacaacctga
    13021 gaaagttaaa catcgccgca atttagtcag aaacgagtta gtattaattg cacctaccga
    13081 aagtaacatt gagccaaaag cgatacaagc ggtcgatttt gccaaagaat tagcaaacag
    13141 ctatttatcg gtgggagacc caaaccacgt tcctgccggc aaatatgcca aaaaagccct
    13201 tagtcattac caattatggg aaagtgttga acctcgttta gcgagggcaa aaaatgtacg
    13261 agatgccctc tcttttgtcg agcgaggcga atccccactt gggattgtct attccaccga
    13321 tgctaaagtg tcgaataaag tcaaagtggt tgccgttttc ccaccggaaa gctacggcga
    13381 aattgtttac cctgctgcgg cagtcagtga aaaagcctct gccgccacat ttttagattt
    13441 tctcaaaacc ccgaaagcaa aagcgaaatt tgaggcagct gggttttatc ctgtaaatta
    13501 aatttactct ctccccttgt gggagagaga cagaaaaata tgtcgttcag acatattttt
    13561 cagagagagg gcaaatagcg tttaaaatat tccccctctc cctgattttt cttctgaacg
    13621 aagaaaaatc tatccctctc ccacaagggg cgagggtaag cttataacca aatagtttta
    13681 gattatgctt aatttcaccc cacaagaact caatgcgatt gttctaagcc taaaagttgc
    13741 ctccattgct gtagtgcttg cattaccgct tgccgtgtgg gtggcgtggc tgcttgcccg
    13801 caaacagttt tggggaaaaa atctgctcaa tggtattatg catctccctc tggtgttacc
    13861 gccggttgtc attggctatt tattgcttat ttcgatggcg aaacgagggg taatcgggca
    13921 atatttgtgg gaatggtttc atttcagctt gagtttttcg tggaaaggag cagtgcttgc
    13981 ctcaatggtg atggcgtttc cgctaatggt gcgttctatc cgtttagcct ttgaggcagt
    14041 tgatcctaaa ttggagcaag ccgccagaac gcttggagca ataccgttaa aagtgttttt
    14101 cacgctcact ttaccgctct ctttttcagg cattattgcc ggtggcgtgc tgggttttgc
    14161 ccgctctttg ggggagtttg gggcaaccat cacctttgtc tccaacattc ccaaccaaac
    14221 ccaaaccatt cctgccgccc ttttcacctt tattgaaacc cccgatggcg aaatggctgc
    14281 cgcccgtttg tgcttggtgg cgattctgat ttcactcatt gccctgttct gttcggaatg
    14341 gctggcagaa aggcaaaaac gctatgctca aacttaaact tcttcagcaa ctcggcaatc
    14401 tgacgctcaa tgtcaatctc gatttgccga ataaaggaat caccgctatt ttcgggcgtt
    14461 ccggtgccgg taaatccagc ctgattaatt taattgcggg gctttcaacc gctcaagccg
    14521 gctttatcga attaaacggt caggtgctgt ttgacagcca aaacggcatc aatttaccac
    14581 ctgaaaaacg caacattggc tatgtgtttc aagagcatcg ccttttccct cattattcgg
    14641 tggaaaaaaa cttaaaatat ggctacaagc ggtcggattc tgcggatttt ttgcaaattg
    14701 tggaactgtt aggcattgcc cctttgctta atcgctatcc ggcaagcctt tccggtggcg
    14761 aaaaacaacg tgtggcgatt gggcgggcgt tattaagctc accgaatatt ttgctgatgg
    14821 acgaaccatt atctgccctc gatctccctc gtaagcaaga gctactggac tatttagata
    14881 aattatccgc tcggcttgaa ataccgattc tgtatgtcag ccacagttta gaggaagtag
    14941 ttcgattggc agatcatctt gtgctgttgg aaaacggcaa agtaaccgca tttgataccc
    15001 tttcagcggt ttggcacagc tcggcatttt ccgcttggca gccaaatcat cagcgaatta
    15061 gcctgcttga attaccggtg gaagaaacac ttccccacta taatatgctt ggcttgagct
    15121 tgaacgcaca gcgattatgg gtgaaccaac tcccccgcca tcaaataggc gagaagctaa
    15181 gggtaactat tgccagcaaa gaggtttcaa tcagcttaca aaaaccgact caaagctcaa
    15241 ttcgtaatgt gttaaaaggc aatgttcgga aaatagaaac gcatttagat cgggttgatc
    15301 tggcaattca agtcgaaaat cagcaaattt ggagtacgat cagcctttgg tcttttaatg
    15361 aattagcgat tcaaccggag caagaggtct atcttcaaat caaaagcgtt tctttatagg
    15421 cttgcaaatt tacgctaaaa tatgaccgct tgttaattcc caaaagagac attatgccaa
    15481 tcgttaatca atctgccctc gtgccttata gtgccgagca aatgtatcaa cttgttaatc
    15541 agtatgaaaa ataccccgaa tttttatccg gctgcgtgag taccaaaacc cttagcaccg
    15601 gcgaaaatga attaaaggca gagttacaca ttcagaaatt aggcattagc caaactttca
    15661 gtacccacaa taaaatgacg ccgccctata aaatcgagat ggcattaatt gaagggcctt
    15721 tccgccattt acacggagct tggacattca ccccctttga tgagcaaagt tgcaaaattg
    15781 ccctgcaatt aaattttgaa ttttccagcc caattgtagc aatggtgttc ggcaaggtgt
    15841 tcaacgaatt aactttaaaa atggtaaatg ctttcaaaca acgtgcaaaa gaggtgtatg
    15901 gtgtctgaac aacgtcaaaa tccagaagaa caaaaaagtg aagaacagaa aattaatgtt
    15961 gaggtgcttt atgcttaccc tgataaatat tttctgaaaa aactgcaagt accgcaaaac
    16021 gcgaccattc aaactattat tttgctctcc ggcgtgttgg aaaaatatac cgaaattgac
    16081 ttgcgtgaaa acaaagtcgg tatttttagc cgcccggcaa aactgaatga aacggtggaa
    16141 gaaggcgaca gaattgagat ctatcgcccg ttaattgctg atccgaaaga aatcagacgc
    16201 aaaagagcgg ctgagcaagc ggagaagaaa tagctttctc ctattcaatc taggggtgaa
    16261 taattattcg ccctatctat tttctccctc gccattcctg ctataaattc cctcctttga
    16321 ttaaatatga aaatctttca taaattttat gtaaattttg agtttgatta tatttaatca
    16381 actttctata gttcactcta aataaaattt tatcaatttt gatgcagagg gagcttatgg
    16441 atattcgtca aaaaattgct cataacaaaa tgacttcatt ccaatggtta gtggttgggc
    16501 ttacggtgtt acttaatatg ctggacggct ttgatttaat ggcactcgcc tttaccgcca
    16561 aaagcatccg agaagaattg cagttagacg gtgcagccat cggcagctta atgagtgccg
    16621 gtttattcgg tatggcgata ggctcactct ttctttcacc acttgctgat aaatttggtc
    16681 gccgcccgtt gcttttaatc ggcacaggct tagccacgtt aggaatgctc ctaacttatt
    16741 tcgctacttc tgcgtttgaa attggtgtat ggcgtgtttt aaccggttta ggcgtagccg
    16801 ccattttaac cggcactaat gtgctagtga gtgaatactc cagccaaaaa tggcgtggct
    16861 tagcgattgc aatttatgcc tcaggctttg ggattggtgc aatgctgggc ggaatgtctg
    16921 cggtgttatt gcaaggagaa tatggctggc gtgcggtttt cctcgttggg tcgattttaa
    16981 ccggtgcggt attcgtttta ttattctttg tattgcccga atcggtggaa tttttaattt
    17041 caaaacaccc ggtgaatgct aaagctcgtt taaatcaaat tgccgttaaa ttaggcttag
    17101 gaaacggctg ggaacttcct gaaattgagg caaaaagtaa aagcaaagtg gcaattaccc
    17161 gtttattcga gcctgaatat cgccgcaata cgttgctaat ttggcttgcc tttattgcaa
    17221 tttcagcctc attttacttt atcagctctt ggactccggc actattagaa gaagccggaa
    17281 tgcctaaaac ccaaagccaa actgtgggaa tggcaatttc agttggtggt acaatcggct
    17341 cgttaatttt cggcttttta gtcagtcgct gggcggcaaa acatattttg caggcattca
    17401 gtattctggc tgcatctgct gtggttgcct ttgtgtttgc cggcaattta gggcttgcga
    17461 taacactagc cattttggtt ggcggattaa ttaacggctg cattaccggg ctttatacca
    17521 ttaacccaac cctatacgca ccggatttcc gcagcacggg cgtaggcacc gccatcggtg
    17581 tagggcgttt aggctcaatt gtttccccaa ctcttgcagg tattttatta gacgccggct
    17641 ggaaaaagaa cgagctttat ttaggtgcag cagtcgtctt attaattgct gctttcacgg
    17701 tacatttttt aaaacagaga aaataagagc taccccgtaa ttaattgagg ttttattcaa
    17761 ttaattacgg ggcaagatta tataattttt ttgattaaat ttatttctca ggtgcttgtt
    17821 caagtagttt ggttaggagt ttccaataaa tctccactgc cggaatatgc actttttcgt
    17881 ccggagaatg ggcgttgaga atagttggtc caatcgagac aaaatccata ttcgggtaga
    17941 ttttgttgat taagccacat tctagccctg cgtgaatcac tttaatttga gattcataac
    18001 ctaaaatttc atcataaatc gctttggtta atggcgtgat tttggaattt gggtttggct
    18061 cccagcccgg gtagttgccc gaaaattcca cttctgctcc cactaatgcg gttaatgagc
    18121 ggattttact gcgaatttcc tcttttccgc cctcaattaa tgagcgggca agaatggtcg
    18181 ctttcacctc actcgcttcg gtgttaagca caccaatact gagtgaggtt tccaccacat
    18241 tttccaccac atcgctatta cgcaccacac catttggcat cacatttaag aaatggatta
    18301 ctcgagcagt gctggcaagg tcaaaggctt gctcaggcag ttgagcttct tcaattaaaa
    18361 attggaagtt tggctcggca attttgagtt ctgcttttac ttgttcggcg gtttggctta
    18421 aacaagcggt taatttttgc tgattttttg cagagaaaac caaggttgcg tgggcttctc
    18481 gtggaatggc attccgcacc gaaccgcctt gaatttctgc aatttggaat tccacttcgt
    18541 tgtgggcttg ttccaaaatg cgggcgaaca ctttaatcgc attcgctcgg gtggtgtgaa
    18601 tatcacagcc cgaatgcccg ccgtttaggc ctttaagcgt aacggttact gcactatctt
    18661 gctgatgagg caccaagaaa attggcagtg tcacattcac attttcccca cctgcacagc
    18721 cgatgtaaat ttccccgttt tcttcggtgt cggtgttaat cataatatcg gcggttagcc
    18781 agttcgggcg taaaccaatc gccccttcca taccggcttc ttcggtcatt gtgagcaaca
    18841 cttcaatggt tgggtgggct aaatcatcgc tatccaacac cgccaagcac gaagccaatc
    18901 caatgccgtt atctgccccc aatgttgtgc ctttggcttt tacccattcg ccgtcaatat
    18961 aaggctgaat cggatcttta gtgaaatcgt gaaccgtgcc ggtattggct tgtggcacca
    19021 tatcaagatg ggcttgcaga gcgacagttt ggcggttttc cataccttta gttgccggtt
    19081 tacggattaa cacattaccc acttcatcac gttcaacaaa gaaatttttc gattttgccc
    19141 aattcacaat aaattcagcc agttcatttt cgtggtaaga cgggtgcgga atagcacagg
    19201 ctttatcaaa ccactgccaa agtagctgcg gtgcgagcgt tgagatttca gacataataa
    19261 gccccttttg taaaatttta agaaaaaata accgcttgta ttcaagctga tatagacctc
    19321 tgacattgta gaatatttag gagaaattta acaaaaaaat cttaaaaagt acttgatgac
    19381 gagttgtttt tttgtacaat atgcgtgtgt tttatacaca tctatttttg tttatcttac
    19441 aaaaggaaca ttttatgtct atcaaaaaag taactttagc cagtttgatt ttattgggtt
    19501 taacggcgtg tagtagtggt ggttcgggca gtagctcaaa caatgtggca aaaccgaata
    19561 ctaaaccgca aactactcag caacaaaccg ataaagcggc agcagaaaaa gcagcaaaag
    19621 acaaggcagc gaaagagaaa gcagaagcag aacgtttagc agcagaaaaa gcagctaaag
    19681 agaaagcgga agcagaacgt ttagcggcgg aaaaagcagc gaaagaaaaa gccgaagcag
    19741 aacgtttagc ggcggaaaaa gcggcgaaag agaaagcgga agcagaacgt tagcggcaga
    19801 aaaagcggcg aaagagaaag cggaagcgga gcgtttagcg gcggaaaaag cagcgaaaga
    19861 aaaagccgaa gcagaacgtt tagcggcgga aaaagcggcg aaagagaaag cggaagcgga
    19921 gcgtttagca gcagaaaaag aagtagatcc tatagctatt attaaagcaa aaggtattga
    19981 tgatacaaat tacggcttaa aacttggtaa tagtaaagaa actattgagg gtaaagtcac
    20041 agtcagaaca cagagttatt tatataatca gccatattct gtagtaacag cacaagcagt
    20101 tcaaagaaat ggctgggaaa acggtaatta tattgataaa gttgatggaa cagttacagt
    20161 taaaggattg aaaacagaga aattaccaac agagggtact gcaacatatt caggaaaagc
    20221 atttaaccac gatggagcta aaggttttaa tggtggtgat ctaacctata atgttaattt
    20281 ctctaagcgt accggttcag gtagagttga gggagaattt ggaaacttaa tcgtattaga
    20341 agaggggacg attaataata ctcagatttc ttcaacagta catggcaata atcctttaaa
    20401 cctttctgga aaatatacag tcgaattttt cggtaaaaat gcagaggaaa ttgccggtga
    20461 aattcagaca aaagcaaatc ttactgggga agtgagtaat tttggacttg ccggcactcg
    20521 tggcgatatc caaaaataac cttttctaac gttcaatgcc gtttaacgaa aaattaaacg
    20581 gcatttgtct tgatattgct tgtagataca ataagacaca atagtttaga tttcaattta
    20641 cagcttctaa taaccctctc ccacaagggg agagagtgat aaatcccgta gaggcgaaca
    20701 tataggtccg cccctaccaa ttttctttat ctttatgaaa aaatatcccc ttttattttc
    20761 gctgttcagc cccgttgtat gggcagaaaa ggtgattgaa cacaacactg acccgatttc
    20821 ctttgagcgt cagccgcaag cggttaaatt tgagcaaaat gttgtaaaaa attcacaaaa
    20881 accgaccgct tatcagcctg cgtattcagt caatcagaat gttgaacagc aaattaacca
    20941 agcgattctc agccaagatt gggcaacgct tgaaccgctt ttaagccgtt atccgcaaac
    21001 tgaaaatttt gacccgttgt tgtggcacta tgcgcaagcc gcacttgcct atgcgaaaaa
    21061 agatcatcaa accgcagttt ttcactatcg ccaactgtta gctaagcccc cgagtttggt
    21121 ttaccctcgt tttgatttgg cgttgatttt ggcagaagat cagcaatttc gtgcggcaga
    21181 gcaggaattt aaggctattc aagcccattt accgccggaa cttgctaaaa ttgcccgcaa
    21241 tactcaagtc caaattgctg aaaatgaggc gtggcagccc gatttttatc tgcaatatac
    21301 ccaaacagat aacgtgaata atgcctcatc ttcgccgatt gtgaatgtaa atgggcggat
    21361 tttccgcaaa aatgcagaca gtttgccaca atcagcaaaa ggcgtgcgtt atggcttagg
    21421 gttgagtaaa ttgcacaacg tgaaaggcaa tcattttgtc ggtttaacgc tcaattatag
    21481 cgggatttac tattgggata accgagatta ccgtgagcaa tccgtatccg tttcgccgaa
    21541 ttatagctac cgcaccgtcg cttatcggct gactttttcg ccttttattg agcaaaattg
    21601 gctggggagt agccgttata atgttcaatt tggggcaaca ctcaacggtt tgcgttacct
    21661 taattcgcaa tggttggtgt cggggagttt tacccatttg caaaaacggt attttgagga
    21721 gctaaccgca agccgttata acagctatca gcaccaactc agtggcggtg tgcagtggca
    21781 agcggtcaaa aatgggcggt tttttgcaaa tttagacggc gtgcgtgaaa ttgcacggga
    21841 aaaagcacaa agctcgaata agtggctggc tcgagtgggg gcggtttttc aaggcgaacg
    21901 ctgggcaacg caggcaagta tcggctttgg caagcgatat tttgccgaca agcactattt
    21961 gtttggctat aagcggttag acacggaata tcaagccaat ttagcggttt ggaaccgcac
    22021 ttggcattgg cagggcattg tgccgaagct gaatttccgt tatcaaaaaa ttgacagcaa
    22081 tattgctgat ttttactctc gccaaaacaa ggaagtcttt ttcactttag aaaagctatt
    22141 ttagtgttgg ttttttgtgc taagcatacg attgcgtagc tgccggcaca aaaaaataaa
    22201 tgaaatgatg gcgaaaaaag cattttcggg ttatcctagt gcaatttttt atatcggggt
    22261 agcccccaat tttatattag gttgtttcta tgagtgaaaa atacacaaat gaaaaatatg
    22321 tcgtgacgtg ggatatgttc catatgcacg ctcgcaagtt ggcagaaaat ttattacctg
    22381 ccacacagtg gaaagggatt attgcggtga gccgtggcgg cttgtttccg gcagccgttt
    22441 tagcacgtga gttgggtatt cgccacgtag aaacagtgtg cattgccagc tacgatcacg
    22501 atagccaagg tgagttaaat gtgttacacg cagccgatac cgatggcgaa ggctttattg
    22561 tggtagatga tttagtggat accggcaaca cggcaaaaga aattcgcaga atgtacccga
    22621 aagctcgttt tgtaaccgtg tttgcgaaac cgcacggtgc accgttggtt gatgattatg
    22681 tgattgatat tccacaagaa acgtggattg agcagccttg ggatttaggc attaccttcg
    22741 ttcagccttt ggcacgtaga taattttgtg taatggacaa tgaaagttgt ccattatttt
    22801 ttatttattc ctatgaaatg taggggcgaa tcatcattcg ccctattccc aaaatcaaat
    22861 aggtatatac aaataatgag tttaggcaat ctttatatta tttctgcccc aagtggagca
    22921 ggaaaatctt ccttaattaa cgccttgttg gcggatttac cccgttcgga agtacagctt
    22981 tcgatttcac acactacccg tcagcctcgt ccggcagaac aggacggcgt gcattattat
    23041 ttcacttcgc acgatgagtt taagcagctg attgagcaag ggtatttttt agaatgggcg
    23101 gaggtgtttg gcaattatta cggcacatca ttgccgatga ttgaacgtag cctagagcag
    23161 ggcattgatg tctttttgga tattgactgg caaggggcta gacaaattcg ggaaaaagtg
    23221 ccgaatgtga aaaccgtttt tattttgcca ccgtcaaaaa cagaattgga aaaacgttta
    23281 atcgggcgtg ggcaagattc cgctgaaacc attgccaaac gtatggcaga ggcggtgtcg
    23341 gaaatgaacc attacaatga gttcgattat gtgattatta atgatgattt ccaacaggca
    23401 ttggcagagc taaaagcgat tttaattgcc gagcgtttga gcttaggttc gcaacaaatt
    23461 cgtcatcagc atttgattac tgatttgtta aataattaaa tgaacagttg ttcatttaac
    23521 tggaaaggca atctgttttt gggtagaata cgcccatttt cccctcttta ttttcaaaag
    23581 gaaatgtaat gaaactttca aaattagcat tttctgtggc gttggctcta accgtaacgg
    23641 cttgtaccac agggaatatg agcaatgacg ggactttgca acaagcacag caaacctatc
    23701 aaagctatga tgaaattacc aaacaatatc aaattaacga gcaatggtgg caaggctata
    23761 cggatgcgga attaaaccgt gtggttgatt tagccatcaa aaataacctt gatttagcca
    23821 aaagcggaat tgcagtcaat aaagccttgt ataacgctaa tttagtaggg gcaaatttag
    23881 tgccaacctt tagtggttca ggttctgttt ctgcggctaa aggtttaggt tcggcttctt
    23941 caaataatgt ttcgaccggt gtttcaacaa ccgcagctca agctgcattt aatttaagtt
    24001 atactgtgga tttatggcga cgtttagccg ataccgccag tgcggcagaa tggacgcata
    24061 aagccaccat tgaagactta aaagctaccc gtttatctat tattaatgcg gttgtgacta
    24121 cttactacca gttagcttac tatcgtgatg cgattcgtat taccgaagag agcattcgcc
    24181 attatgagca aatcagccgt attttaacta acaaaatgaa tgccggtgcg attgatagtt
    24241 tagcggttga tcaggctcaa caagcggtct taaacgccag aaatagtgta attgatctga
    24301 aaaccaacca aaaaattgcc gagcaaacat tacgcaattt gttaaatgtt cagccaaata
    24361 ctccgcttaa cattcgttac ccgaatatta tgagcgtgaa attgcaaggg gtagatttaa
    24421 atgtgccagt gtcagccatt gctaatcgcc ctgatgtagt aggctcgtta caacgcttac
    24481 aaagtgcatt taaaacctta accgcaaccg agaaaagctg gttcccaacc ttaaccatcg
    24541 gtggttctat taccggtagt gcaaccaata tcggcaatgt ggcagataac cctgtgggta
    24601 acggcttaat taaattggat ttaccgttct tagattggaa tcgggttaaa aataacgtaa
    24661 aaatttctga aacggattat gtgactgccc gtttaaacta tgagcaaaaa attacctcag
    24721 ccttaaatga aattgaccgt tattattaca gttatacgca gtcgagaagc tcgttaaaaa
    24781 tccttcaaga taccaatgcg aaaaataaac gtatcagttc atactataaa aaccgttatg
    24841 aacaaggggt gtcggagttc cgtgagtgga ttaccgcact gaataccgag cttaattctc
    24901 agttggcgat tttaaatgca aaatatgcga ttttgaccaa cgagaatgcg gtttaccaag
    24961 caatggcagg taaatacaga agataattat atctaaaact atatactatg gttggtaaaa
    25021 gtgggttaag gcttgatggc ttaacccgct tcgctattta ttgctactta aaccattttg
    25081 gtaatgttag tttagctact agaacttaat cacatcaccg tttttcggca cagttacacg
    25141 atttaatccc tctttttgta cataatcacg catctgatca ctgctgattg aggtttgtgg
    25201 cagggcgtcc atatgcacag ttaagatttt cgcttgtgga gcgattttgt gtgctttgga
    25261 aacatcttcg gtacacataa tcgggctgcc ttcaaagttt ggtagtttgg cacagccggc
    25321 attcagcacc acaaagtctg gtttgtaagt gttgaacgaa tgttcgattt cgtggttcca
    25381 aatggtatct cccaccaaat agacggtcgg tttgccttcc gctttcatca ctacgtccat
    25441 cgcatcgccg accatttcgg caattttcgg gttcgcatac atcgcatcgg tgccgtgctg
    25501 gccacgagtt cgggttaagg ttacgccttc aaacggggta tctttgccga ccacacgcac
    25561 atcggtaaag ccttgtgaac ggattaaacg ggcatcaccg gcattctgca caaaaatcgg
    25621 cagatttttc ggcacacgtt gttgtgctac atcgtcccag tgagcgtggt gagtgtgagt
    25681 tacgatcacc gcatccacgc catttaacac ttcatcaatg ctcacagtca tatccaaggt
    25741 cggattacgt tttttcgcat tcggcggtaa gtagcgatct ttctccgcta aatacggatc
    25801 aaccaaaaac gtcttgccgg catattcaat tttcgccgtt gcattacgaa tatgttggta
    25861 ttggctctgc tcagcggcaa aacttgatgt agcgaaaagt gccgttgcaa tcaggctcag
    25921 tgctgttttt ttgacaaaag tcataggttc tccaaaataa attaaaatgc agagtatgat
    25981 tttttacgct ctgcaatgct ggtttgcata aggaacagtg tcccttacat caataaaagc
    26041 ggtcgatttt gcacattttt ctgcaaaacc aaccgcttgt tcggctaggc taatttctct
    26101 accttcgccg gaatgccatt tctcaccgct gagccacaga cccaatctac ccacggggat
    26161 ttgaccgctt tggtgtggtc gataatgccg aggtcgttga ggttaatccc tttggcgatc
    26221 atcgggttgg ctggaatttc cacgccgtca atggtgtagg aacttgcccc aatctgttta
    26281 tgaccgtagc cgtgttcgat gccgattgtg cctcgggcga taccgtccaa caccacaatc
    26341 tgcaccgtca agccggcggt tggggtgctg agtttcacca catcgccgtg tttaatgcct
    26401 ttttcttccg catctaaacg gttcatcgcc actaagccct caggcttgat cgagagcaga
    26461 cgcaacagtg gggcggtaat actgctcata atgttcgatt taaacgaaat cagtttaaag
    26521 ccccattccg actgcggata gtgctgctcc agcgtggagc cgtccgcaaa acgtggcgac
    26581 atataacgag ggcagcccca atagtgttca ccgttgctgt ggtgctgggc tttatacacg
    26641 ttttcgttcc aaatttgcag gcagtttttc cactggaact ccatattctc ctctttccac
    26701 gcactttcgt gtggggcaaa acgtccgcct ttgcaataca cattcgccac ttttaatcgc
    26761 tcttcggctt ttagcgtttg ttcaattttc ggcataatcc gttgcacgcc ggtgagcagc
    26821 agatcttgct cgctggcgtc cggtgcaggt ttctcaccgg caaaggcaat gttcgctgcc
    26881 gcccgtagga agtaatcttc ggtacggtgg agcggatagc ggttgccttc taaatcggca
    26941 atcgcatttt cgccaaagcc cggcaagccc atttttttcg ccaccgcaat cacgaaattc
    27001 tccatacaga tggtgtcgcc ttcggcagtt ttggcgtttt tggacggcac aatcgggtgg
    27061 cgtgcggtgc tggtgcgttg aggcacaccg ccccacgccg ttgaaaagcc ccagctctca
    27121 aagttgtggg tgtccggcac gatgtagtcc gtcagtgcag tggtttcgtt cataaaagca
    27181 tcaatagaga caaagagcgg tagaattttc ggatctttta atttctccaa cgcaatcggg
    27241 ttcatagcgg ttaagccgta aacggggttc gccatatggt tgatccacgc tttcgcaaag
    27301 tatggatagc cctgcaccat tgaggtaaac atctcggtag tttgcccgcc ggtgaacgga
    27361 taccacgccc cttgtgccgg atacggattt tcgcccttcg tcactttctg cttaaattcg
    27421 cttgaatttt ggtacgcctt tttactgcgg gcaaggttcg tgccttttgg cttcacttca
    27481 ttcgggaagt tcgctaaatt gtagcgaggc ccgtccccga aatctttgaa tttgcctgcg
    27541 gctactgcca ttccgccttt tttgttgagg ttaccgacca ttgcattcag caacaaaatc
    27601 gcccaactgg tataaaaacc ggtgctgtgc atcgtgccgc cgtgagtgat ggcattggct
    27661 ttatgaccgt gcgaagtgaa ttttttcgct aagttttcaa tggtttgcac cggcacgcca
    27721 caaatgtcgc tatattcggc aagggttttc tcttgtgtcg cctctttcag caactgcatt
    27781 gaggatttca ccaacacttc cgagccatca agcagttttg caatttgttc gacaaaaatg
    27841 accgcttgta agcaatcttt tgctgaaatg agttcgccgc ttgcctgatc ttttaccaca
    27901 atcaggtttt cttccacttt ctcttcgggg tcgagttgca catcttgcag atcgctaatc
    27961 cgcaacgcct gcccgaattt cgggtggttc ggttcgcaaa taatcagatg tgacgcattg
    28021 gcatagctga cgtggccggc attttccatc gctgccagac ttgggcggga gaggtagtca
    28081 gcattgaaac gctcgttttc gataatccaa cgcaacattc ccattaccaa cgccaaatcg
    28141 ccctcgggct taatcggcac ccaatggttg ttttgggtgg cgagggagga ggtcatctcc
    28201 aaacgagggg aaaccaccac atagctgaaa ttgtcatcag ggcggcgttt ggcaagctgg
    28261 cgagcctgac gcttaaacgg attgcccgac tgtgccggag acgtgccgat aaacagaata
    28321 aattcagcgt gatcccagtc cggcttggcg tgttgttggt tgtcaaaatc tttcataaag
    28381 gcacccgaac cggcacggta cgcagcgcca caataagccc cgtggttgga aaagttgatc
    28441 gtgccgaagc tcttttgggc aaagcgttgc aaaatcggct gacgaccttc ggggccggca
    28501 aaagtaacta aaagctggtt tgctttcatt cccaaatcag gctgtttcgg gttaatcgga
    28561 tcgaccagat tgcggatcgt cttcaagccc tccacttcgc cttcaccaaa taggttacca
    28621 ccattgatga tttcctccac caactgctca aagctgatgg tctgccactt gccttcgccc
    28681 cgttttccga cccgtttcaa cggttgggta atccgatacg ggctgttcac cccctccaag
    28741 aacgccgccc ctctagcaca ggcggtggag cgattttcaa tgccactttc gcccgccagc
    28801 aacagttccg ccttggcaat cggcataccg aacggcaggg ttttgtcgga ggaaagcggg
    28861 tgatacggat tgcccgaaat gcgaatcact tggttggtgt tcaaatccac cctcgcccga
    28921 atgccacact gcgtccaaca gcccatacac tgtgtattgc acacaatttg ctcaggattg
    28981 ctgagcagtt tgccgtcttt tacctgatac tccggcgtga gcgaattacc gtgaattgca
    29041 tcacgggttt tctcgcccga tgtgccgtgc atcacccctt tggcaatctc cgacaccttc
    29101 ggcgaatacc cggccccaaa cgccgccgcc ccaccaagtg ccagcccgcc tttcaacaga
    29161 tttctgcgtt tattgttcac cttttcttcc cccacaaatt attaaaatca gttgccatac
    29221 cacgatactt actagcaccg ttagcccaaa aatcgagaca atccccaaac caccatcgac
    29281 ggctgtccaa tcgaaatgat atggattttg caaggcattg gttttggcaa tggtttggac
    29341 ttggatcacc agtacccagc ggagtagcca gccgaaagcg atgagggcaa gcacgccgag
    29401 ccattgcaag cggtggtttt tggcagaaaa attgcaaatg agggcaatta gccagaaaat
    29461 gccgacaagg taagcggtga ttgagccatt gaagagctgg gcaaaatctt gtgtggcttg
    29521 tggactactg aatgctagtc cgacaagcga aagtgcggtt aaaatcaggc agagttttgt
    29581 ccatagcccc gtgttggttt gggtgtggaa cagcattttg cagaccgctt gggtaagccc
    29641 caatgccacc gcccacgcag aaaagagcat aatcggcagc agccacgcac tgtgccataa
    29701 ggttcttgtg ccggtttggt aggtttcaag cacggtgtag ccgataatgc cgatagcggt
    29761 gagtgtggca aacaggcgga atgcccaaag caagccttga ttttgcagta tcggcaactg
    29821 tagccacgct aaccatttcg gcaggttcgg ccgggtatgg tgagccaatg ccagcacaaa
    29881 ataacccgct accgccacgc tgaacagggg caggataatc gctccccacg ccattggaga
    29941 tcgccacgca aaatcggtgt agaaatgcag aatacggctc ggttggtgca ggtcggcagt
    30001 caatgccaca ggggcgacaa tcgagccaat caaggctacc gtcaccgccg ccagctcgca
    30061 agcggtcggt ttcagtgatt tttttgcaaa cagcacggcg gtaaacactg ccgttgccgc
    30121 cacgcctacg aagaagaaat aatgcacaat ccacggcaac cagacgatat gttgcggttc
    30181 cactaatact tcacgaatca ttttacgcct cccccgtcca gagcattggt tgcccgtcaa
    30241 ttttgctcac aaaggcatca tcgtctagcc ccacatagaa aacgtgtggc acggtgccgg
    30301 cctccggttt caggactttc aaatcatctt tgtgggcttg ataaagttgg gaaatttgac
    30361 ttttcgggtc gttcaaatcg ccaatcaccc ttgccccgcc cacgcaactt tccacgcacg
    30421 ccggcagcag acctacctcc aaacggtgag tgcagaaggt gcatttatcg gcggttttgg
    30481 tctcttcatt gataaaacgg gcatcatacg ggcaggcttg cacgcagtaa ccatagccga
    30541 tgcactgctc attgttaatc accaccacac cgtctttacg ttgaaaggtt gcctgcaccg
    30601 ggcagaccgg cacacacggc ggttgatcgc aatggttaca caaacgaggc aacaccacat
    30661 tgttcaccac ctgcgtgccg ttggtgattt catattgacg caccgtggtg cggaaactgt
    30721 gcaacggcgt ggcgttctcc accgaacagc tcacggtaca cgcttgacag ccgatacatt
    30781 tacgcaaatc aatcagcatc gcatagcgtt tgttctcatc gccctcacgc caagccggca
    30841 taaacgcctg ggccgattgc aacggcacaa aactcgcccc caccgtcaag gcggaaagct
    30901 ctttcaaaaa ggaacgtttg cttaaatcca tatttccccc acaaatttaa aaataacaaa
    30961 ggtattctaa ggcggagagg gaacggcaag gtattgtggt tttccacata tcaaggggaa
    31021 agttgatttg ggtcaaggat gaagggaaat gccctataca agtagataat agggcattga
    31081 tactaattaa aaagcaataa gatggtttag tttagatcta aagtttttat acgcattaat
    31141 atcactcggg gtactaggta tcgctaatct cctagaattt ggggcaatta atatattatg
    31201 tttctttcct tttctaattt tc
//
LOCUS       DANLUQ010000010        29515 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.010, whole genome shotgun sequence.
ACCESSION   DANLUQ010000010 DANLUQ010000000
VERSION     DANLUQ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 29515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29515
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.010"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..1334
                     /locus_tag="PXB27_000390"
     CDS             <1..1334
                     /locus_tag="PXB27_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HDL5345219.1"
                     /translation="KQNNFHIFEKFGIFSSFWAFPVLFLTLEQQTTNDSRYLNRISEM
                     LLNWILSPALIIYTAIIYAYVIFIAAKGQMPDGVVANVALPYLAFGLAIQAVQLLLKN
                     AKWQWFYSHFTYLALLPLTLIWYAIYIRFSHYGLTEARIYLVIGTITLSICYTLLLFK
                     QLAQYRLFSAVSIAIILISVFILDPKTVALKDQTKRLDNYLLKHKLLDVNNKISTKAV
                     LKYQESLNENRDEAEYFYSMLHYVARGMPAAQFKEKYGVNSSYDLMSEIRERNTDPRY
                     FEATNSELVRLTKADMANAREYITFSSQLYRPNSFSHQKIENKLCQKLLSQGYYFEGN
                     ILNIEYQYSETAQDCSSITQDPTEFVIYFKGLGTTIEFNINDILKKIFAKHQLSITQQ
                     HKNEVLEKIKGDLLKMDLGNSVLGLKHIELRFEDNIGYIVESISTQYIMLK"
     gene            complement(1377..4406)
                     /locus_tag="PXB27_000391"
     CDS             complement(1377..4406)
                     /locus_tag="PXB27_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4981 domain-containing protein"
                     /protein_id="HDL5345220.1"
                     /translation="MSLPNYYQDPSVLHLNTTLHHAYFIPFAKGQTPNQLEREQSELF
                     TLLNGDWKFNFYPSPHDLPEDFLNTKFTHQIPVPSNWQTQGFDAHQYTNINYPIPFDP
                     PYVPHQNPCGVYQREITFNPKPNKRYLLNFEGVDSCLFVYVNGEFVGYGQISHSTNEF
                     DITKQLKQGKNSLVVVVLKWCDGSYLEDQDKFRMSGIFRDVYILEREQNYLQDFFIRY
                     QLNADLSKAELKVETQFSTYTEAVHFQLFDPENRLVCEQTSGDFCQEICNLCLWNAED
                     PTLYTLCLSYQNEIIEQKIGFRQIRVENGVMLFNNAPIKFKGVNRHDSDPKTGYSISR
                     EQALVDLRLMKAHNINAIRTAHYPNAPWFSELCDQLGFYLIAESDLESHGSSAVYIES
                     PENSIFLNVPNPNKQEEIRQQAVDNFCYFARDPLFKQAILDRNIANVERDKNRTSVII
                     WSLGNESGFGENFEVAARWVKQRDPSRLTHYEGSIYQHSAHQNDLSNLDLYSEMYPST
                     ERLEEYFSDSANHKPFILCEYSHAMGNSSGDMEDYWQTFQRYSGACGGFVWEWCDHAP
                     LLPNSELSAEQKTEKYGYGGDFGETLHDGNFCMDGLVSQQRVPHSNLLEVKNVNRPVR
                     AELKAGKIWLKNQLDFSDLADYLTVHYCFSENGETTLQGQCLVNCQPNQTACLPLTLP
                     EPNGKLQLLTLDYSLNESTALLEKGHHLGFDQITLHQAMVTPALNPRPQAVIFVQDFA
                     NEWRIQQGDYRYTFDKLKGIFSRIEKNGEAVIEQPLDFNIWRAPTDNDRLIREFWKKA
                     GYDNCYTRAYHTELMQDKNGVNITAECGIVATAQSRILTLKVNYHLAPNGEMTIQVQA
                     TRPAHLPFLPRFGLRFFLNKRNNQVSYFGYGEQESYIDKYHLAKLGAYQTSARENHTD
                     YLKPQENGSHFGTHYVQANGITVTSPQPFSFNLSPYTQEELTTKSHSYDLVESPSTVL
                     CVDYKMSGIGSNSCGPNLKPQYRLNETNFEWLLKLSF"
     gene            4584..5441
                     /gene="menB"
                     /locus_tag="PXB27_000392"
     CDS             4584..5441
                     /gene="menB"
                     /locus_tag="PXB27_000392"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005723289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HDL5345221.1"
                     /translation="MLYPSEEFLYAPVEWVDHSEGYTDIRYHKSKDGIAKITINRPEV
                     RNAFRPQTVKEMIEAFSDARFDEKIGVIVLTGEGEYAFCAGGDQKVRGDYGGYKDESG
                     VHHLNVLDFQRDIRTCPKPVVAMVAGYAIGGGHVLHMLCDLTIAADNAKFGQTGPKVG
                     SFDGGWGASYMARLVGQKKAREIWFLCRQYNAQEALDMGLINTVVPYADLEKETVRWC
                     REMLQNSPIALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNEKREP
                     DFSKFRRNP"
     gene            5524..7926
                     /gene="mrcB"
                     /locus_tag="PXB27_000393"
     CDS             5524..7926
                     /gene="mrcB"
                     /locus_tag="PXB27_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1B"
                     /protein_id="HDL5345222.1"
                     /translation="MSDVELNEQSEQEPEQKPKKRNYFLSTLFKIGVIGACSMAFYGI
                     YLDGKIRSKMDGQIWELPAEVYARIESIKIEDNLTLEQTKQALLDNGYRQVSQVATPG
                     DFKIEENQLVLLRRAFPFPETPESQRVLRLTFKQDKLTYIEDLVQGKLIEEFRLDPKL
                     LALLHSDSDEERQALRLQQYPRLLIESLILTEDRRFYQHDGISPIGIARALITNYQAG
                     RTVQGGSTLTQQLVKNLFLSNERSLVRKVNEALMSVILDFRYDKNRILETYLNEIYLG
                     QNGSYQIHGFALASQFYFGRPIQEISLDQIALLVGMVKGPSLYNPWRNPQGAVERRNV
                     VLRLLLDHQVINQELYALLSSRPLNIREKGTIYREQPAFMQALTIDLKNELGEIKSAQ
                     LSGTKIFTTLDRKQQRSAEQAVITGLEDLESKNKKVSDLQSAIVVAEYRTGKVRAIVG
                     DRLTQFAGFNRAIQSKRQIGSLVKPSVYAIALAQPELFRLNTPIQNKQISIKVGSTYW
                     TPRNYDRSFSGSVMLMDALVRSLNIPTVNIGMKVGLKKVIAKQKEMGWDNAEIPPYPS
                     TLLGSYSISPYDVTRSYQVIANEGLKTPLTTIEAIISHNGEMFYQRDSGKVSEQVLPP
                     EAAVQTMYAMQQVVERGTARSLQNDFAKLKLAGKTGTTNNARDTWFVGIDGEHVTTVW
                     LGKDNNGDTNLTGSSGALYVYKQYLERALPQAYRLPKLNTMQWVGINSYGSWNCDSGR
                     QIPVWRDKGQRYCTSAGNVVATEKPSVWDALSLKKENIEAKAEEAEGKQPIPTEEVAP
                     IE"
     gene            8101..8607
                     /locus_tag="PXB27_000394"
     CDS             8101..8607
                     /locus_tag="PXB27_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HDL5345223.1"
                     /translation="MAMSNIGLDKEIAKKLADELNGLLATYQVFYTNVRGYHWNVRGV
                     NFFELHAKFEEIYDDLVTKVDEIAERILTLGHTPQNAFSQYLTKSQIQEHIGVSAAQE
                     CLRGTLNGFKTLLGQQREILALANDANDEGTASQMSDYIKEQEKLVWMFTAACAECSD
                     YAQGCSCN"
     gene            8666..9379
                     /locus_tag="PXB27_000395"
     CDS             8666..9379
                     /locus_tag="PXB27_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HDL5345224.1"
                     /translation="MAMSQGFQFKQFFIRHDRCAMKVNTDGILLGAIAHIKDCQNILD
                     LGIGSGLVALMLAQRTDDCCHITALELEPNAYQQAVENAQNSAWANRISVLQGDVMQQ
                     AFEQKFDLIVSNPPYFADSLAARTTERDLARSISHYSHLNWLAKAKPWLAENGKITLI
                     LPIAAAEKLVDQSEQIGLYCVEQWLIFTKSGKLAKRSIVSFSLNKQKREHKELIIYDL
                     DNCYTTEFKRLTQDFYLNF"
     gene            complement(9490..10494)
                     /locus_tag="PXB27_000396"
     CDS             complement(9490..10494)
                     /locus_tag="PXB27_000396"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005651072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDL5345225.1"
                     /translation="MAFNLKNRHLLSLVNHTEREIRFLLDLARDLKRAKYAGTEQQKL
                     KGKNIALIFEKTSTRTRCAFEVAAYDQGAHVTYIDPNSSQIGHKESMKDTARVLGRMY
                     DAIQYRGFKQSVVQELADYAGVPVFNGLTDEFHPTQMLADVLTMIENCEKPLSQISYV
                     YIGDARNNMGNSLLLIGAKLGMDVRICAPKALLPEDAFVKMCEKFAAESGARMTVTED
                     IETAIKGVDFVHTDVWVSMGEPLESWTERIDLLMPYRVTPELMQATDNPKVKFMHCLP
                     AFHNSETKVGKEIAEKYPALANGIEVTEEVFESPMNVAFEQAENRMHTIKAVMVASLA
                     "
     gene            complement(10582..11421)
                     /locus_tag="PXB27_000397"
     CDS             complement(10582..11421)
                     /locus_tag="PXB27_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDL5345226.1"
                     /translation="MQSYPYLLVNVFATSHFGGNPLAVFYEADGLTDLQMQLIARQFN
                     LSEVVFIQSSTDSQAVKKLKIFTPDYEMPFAGHPTVGAAFVLHSAFDFPTTYRLQTNA
                     GLVAIQHQHNVTTFALTNGVNLENTPLSRAECASILGLNVADIASEPCYVNTGVEQLL
                     VELASQQAVEKCKINANLAEKAFLKDSVYVWCREGNEAKVRLFFQSQGSIVEDPGTGS
                     AAANLGGWHIAQGKIPIQLQISQGDEIQRPNRLLLKVDQNGKIFVGGEVILVGKGEFY
                     LPE"
     gene            11611..12945
                     /locus_tag="PXB27_000398"
     CDS             11611..12945
                     /locus_tag="PXB27_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HDL5345227.1"
                     /translation="MLYAEFLLLLAFLYAGSRYGGIGLGVVSGIGLFVEVFFLGMPLS
                     APPISVMLVILAVVTCASILEAAGGLKFMLQVAERILRSNPKRITFLGPLVTYLMTLM
                     LGTGHSVYSIMPIIGDIALKNKIRPERPMAAASVASQLGITGSPLSAAVAYYLTEIVK
                     LPGFEGVTLLNVVGVTITATFCGVIAMSLYSLRRGKELEDDPEYQRRMQDPELRKQIE
                     QTSATSLDEKLPASAKNSVYLFLAAIATIVIIAMIPAIKPTVAATGKVAGMDQIIQIV
                     MLTFGGLILILTKTDPKKVPNGIVFKSGMVATIAIFGIAWMSDTYFTHAMPAFKEGVT
                     EMVRAQPWTFAFALFAVSVVVNSQAVTAKMLLPVGIAMGLPAPLLVGLMPATYAYFFI
                     PNYPSDIATVNFDVTGTTKIGKYYFNHSFMAPGLVGVITACIVGIAVANVLI"
     gene            complement(13025..13435)
                     /locus_tag="PXB27_000399"
     CDS             complement(13025..13435)
                     /locus_tag="PXB27_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDL5345228.1"
                     /translation="MNSTLTNLQPLAFLITRVVAGYMFLLHGTAKFFEFPMSMTNGNG
                     SVPLASIYGVGGILEIVGGIFFILGLFTRQTAFILSGMMAYAFFIAHGAQGNVLLPLL
                     NGGELAGVYSVLFLTYIFIGGGKYSLDAKLFNKA"
     gene            complement(13558..14235)
                     /locus_tag="PXB27_000400"
     CDS             complement(13558..14235)
                     /locus_tag="PXB27_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDL5345229.1"
                     /translation="MNLFGFRCFHCNHPLAIGVQGICSHCQKLLKPTPYCRHCGMSAV
                     EFRNACGYCLKNEPKWHKLVRVNEYRAPLSHWVHQFKFQQQYWWDQALARQLLLAIKQ
                     AQREQVLSLPEVIMPVPLFWHRHWKRGFNQSELLCDWLSKWLQIPLDTQSLQRVRATV
                     SQRELTAAERRKNLRGAFFYQPLKAYKRVAIVDDVLTTGSTLNVICAELLKAGVVEIQ
                     VWVLARA"
     gene            14353..15165
                     /locus_tag="PXB27_000401"
     CDS             14353..15165
                     /locus_tag="PXB27_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL5345230.1"
                     /translation="MKSLQKPIKIVFFDIDDTLYVKHKAYIPKSITEQVLPRLKAKGI
                     IPAIATGRNHGCFPEALKPYLNPEIGFELFVTINGQYNFYKDQIISDYSLSREQIERI
                     IEKANALGILYAFITPMTIAVSETNPIVHEAVLPITPNYLIDPHYYEKEKIVQMLLFY
                     TEEQKQAVENSGIFANDLKEARWHPNAVDILRKENSKARGIQDVLATLNIEIESAMAF
                     GDGINDLEMLPTVGVGVAMGNGVPELKAVADFITKPLEEDGILYALETLEVI"
     gene            complement(15213..15482)
                     /locus_tag="PXB27_000402"
     CDS             complement(15213..15482)
                     /locus_tag="PXB27_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDL5345231.1"
                     /translation="MGLLYLLLLNTLIGYGSYGEALNHWDTSKVSIVTTMLPIFTMIF
                     SNLSYYFYPHIFAKPDMNWISYLGALVVVSGAILAIAGDKLFRRN"
     gene            complement(15455..16132)
                     /locus_tag="PXB27_000403"
     CDS             complement(15455..16132)
                     /locus_tag="PXB27_000403"
                     /inference="COORDINATES: protein motif:HMM:NF013088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL5345232.1"
                     /translation="MKQRPLLGFSLALTAAMTWGTLPIAAQQVLKVIDAQTLVWVRFV
                     VASVGLLLILGFAKRLPKLTACSKKELGLILLGVCGLSANFFLFAEALHYVSPTINQV
                     LWQLAPFSMILLGVLLFKEKFGLFQKIGFVLLIAGLVAFFNDKFAEILQLNTYALGVL
                     LSAGASLIWVLYGVAQKLLLAKFNSQQVLFIIYIGCSLLMLPFASPAQLGNLEDGFLW
                     GCFIYCC"
     gene            complement(16234..16491)
                     /gene="rpmA"
                     /locus_tag="PXB27_000404"
     CDS             complement(16234..16491)
                     /gene="rpmA"
                     /locus_tag="PXB27_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDL5345233.1"
                     /translation="MATKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGSNVGMGKDHTLFATADGKVKFEVKGEKSRKYVSIIAD"
     gene            complement(16512..16823)
                     /gene="rplU"
                     /locus_tag="PXB27_000405"
     CDS             complement(16512..16823)
                     /gene="rplU"
                     /locus_tag="PXB27_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDL5345234.1"
                     /translation="MYAVFQSGGKQHRVSEGQVVRLEKLELATGEKVEFDSVLMVVNG
                     EDVKIGAPVVAGAKVVAEVVAQGRGEKVKIVKFRRRKHSRKQQGHRQWFTEVKITGIQ
                     A"
     gene            17101..18099
                     /gene="ispB"
                     /locus_tag="PXB27_000406"
     CDS             17101..18099
                     /gene="ispB"
                     /locus_tag="PXB27_000406"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HDL5345235.1"
                     /translation="MAVQLFSLEQIQALANEDMQAVNAEILAQLNSEVLLINQLGHYI
                     IAGGGKRIRPLIAVLAARALGYRGKKHITAAAFIEFVHTATLLHDDVVDESDMRRGRP
                     TANAHFGNAASVLVGDFIYTRSFQMMTDLDSLRVLKVMSAATNALAEGEVQQLMNVND
                     PNTTEANYMRVIYNKTARLFEAATQCVAIAADASEEIETALKNYGCYLGTAFQLIDDI
                     LDYSAQSESLGKNVGDDFVEGKPTLPLLHAMHAADKAGKTTQAELIRNAIENGGELEQ
                     IPQVLAIMQEHQSLDYAMQKAREEAQKAVDSLGVLPESEYKQALVSLAYLSVDRSY"
     gene            18202..18948
                     /locus_tag="PXB27_000407"
     CDS             18202..18948
                     /locus_tag="PXB27_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HDL5345236.1"
                     /translation="MTEQNQRPQEHIESVRARRRAQNQKKGPNAPFVREKLTLPNGHN
                     KLLLHSCCAPCSGEVMEAIHASGIEFTIYFYNPNIHPLKEYLIRKEENIRFAEKWGIP
                     FIDLDDDYENDRKEWFKKAKGMEWEPERGIRCTMCFDMRFEKAAEYAHNNGFPVFTSC
                     LGISRWKDMNQINDCGHRAAAKFDDVVYWDYNWRKGGGSQRMIEISKRERFYQQEYCG
                     CVYSLRDSNKWRLETGRQRIEIGKLYYSPD"
     gene            19365..20300
                     /gene="fabD"
                     /locus_tag="PXB27_000408"
     CDS             19365..20300
                     /gene="fabD"
                     /locus_tag="PXB27_000408"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDL5345237.1"
                     /translation="MSKFAMVFPGQGSQAVGMLADLAPQYPIVEETFKQASDVLGYDL
                     WDLVQNGTAEELGQTHRTQPALLASSVALFRIWQQKFPEQKPAVMAGHSLGEYSALVC
                     AGVLDFQDAIKLVELRGRAMQEAVPAGSGAMFAIIGLDNESIIKACEQAASETGEIVS
                     AVNFNSPGQVVIAGTKAAAEKAGELCKAAGAKRALPLAVSVPSHCALMKPAAEKLAEA
                     LQNIELNQPLVPVINNVDVAVETDADTIRNALVRQLYSPVRWTETVEKMAQDGITTLY
                     EIGPNKVLTGLAGRIVKELSAKAVNDVASFEAVEA"
     gene            20317..20781
                     /locus_tag="PXB27_000409"
     CDS             20317..20781
                     /locus_tag="PXB27_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345238.1"
                     /translation="MSKETLFMLISALIALVILPLAYTVGIAKETLGGNMGMLSIAAG
                     CSFLAYFLSLNPKIRHAFIGGSAITAAAIQAWVLYGVLLSENDPKSAVVLFQIYLFSS
                     LGPLFFSAIWAFTRPRWIYPKQGSAVKYALFGGLTTVINYLITLFIFYILTL"
     gene            20802..21524
                     /gene="fabG"
                     /locus_tag="PXB27_000410"
     CDS             20802..21524
                     /gene="fabG"
                     /locus_tag="PXB27_000410"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HDL5345239.1"
                     /translation="MSKIALVTGATRGIGKAIAEELVAKGYIVVGTATSEKGAESISA
                     YLGENGKGLVLNVADAESIESVLKQIKETFGDVDVLVNNAGITRDGLLMRMKDEDWFD
                     IIQTNLTSVYRLSKAVLRPMMKKGGRIITIGSVVGSMGNPGQTNYCAAKAGLVGFSKS
                     LAKEVASRGITVNVVAPGFIATDMTDELTEDQKNAILSQIPAGELGSPQDIAKAVAFL
                     ASDDARYINGETIHVNGGLYMS"
     gene            21643..22941
                     /gene="brnQ"
                     /locus_tag="PXB27_000411"
     CDS             21643..22941
                     /gene="brnQ"
                     /locus_tag="PXB27_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDL5345240.1"
                     /translation="MTRNTLVVGFLLFAIFFGAGNLIFPPKLGYESSTEFFSAISGFV
                     LTGVGLPLLGIIVSAFYNGGYKSALDKIHPWFSLAFLTSIYLAIGPFFAGPRTAATAY
                     EMAIMPFLGDSSQVSMLIFTLIYFAFTLWLALNPNKMVDRIGAILTPILLITIIGLVI
                     RAGFILDTDPQITTPNTNEQSYFFKGMVDGYLTMDALASLAYSVIVLNAIRGKGVSQA
                     ALQRQTVSAGLVAAVALAVIYISLGWIGNNMPISANTLSMLSERKLDLGTYILNTVTT
                     QAFGELGRSVLGVIVSLACLTTAVGLAVSVSEYFNEIFPKISYKVYAILFTVISFTVA
                     NQGLKEVISKSIPVLLVLYPIAMTVILMLLINLVIKLPLSAQRLSLLLVTIVAIASVA
                     GVGFTENLPLKSYSMEWLPFAIIGAIIGAIIHPLTGRKHK"
     gene            22987..23811
                     /gene="mutM"
                     /locus_tag="PXB27_000412"
     CDS             22987..23811
                     /gene="mutM"
                     /locus_tag="PXB27_000412"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HDL5345241.1"
                     /translation="MPELPEVETSVRGVSPYLVGQTIEQIIVRQPKLRWTVSPELSQM
                     AGAKITAVERRAKYLIIRTDKGDILVHLGMSGSLGILQERQQKEIGKHDHVDLITQNG
                     TILRYNDPRKFGCWLWAEKAEDHELLRKLGPEPLSNDFTADYLFAKSRKKTTACKTFI
                     MTNEVVVGVGNIYACESLFMAGIHPELAAQNLTKKQCERLVKTIKEVLTKAIIQGGTT
                     LKDFIQPDGKPGYFAQVLQVYGRKGETCNDCGKPIETKVIGQRNTYFCPKCQRLPK"
     gene            complement(23894..24328)
                     /gene="dtd"
                     /locus_tag="PXB27_000413"
     CDS             complement(23894..24328)
                     /gene="dtd"
                     /locus_tag="PXB27_000413"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDL5345242.1"
                     /translation="MIGLIQRVKFAKVEVEGQVVGEIASGLLVLLGVEKDDDEAKADK
                     LLEKVLNYRVFSDSEGKMNLNVQQAGGSLLVVSQFTLAADTNKGLRPSFSKGASPVDA
                     ERLYAYFHQQAATKIHTQTGQFAADMQVSLQNDGPVTFWLQV"
     gene            complement(24303..25148)
                     /locus_tag="PXB27_000414"
     CDS             complement(24303..25148)
                     /locus_tag="PXB27_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HDL5345243.1"
                     /translation="MLNRLWRFIKLFAKRYNENNIAISAGYLTYSTMLAIVPFIVVLF
                     SIFTFFPIFHEANEQLKAFIYDNFAPSASEMVSEYIELFVSNAKQMGLVSFIGLFVVT
                     IMLIKSIDEALNTIWHDNRQRPMWFSFLLYFLILLFGPLLAGASIAISTYVMSLSIFN
                     GNGALSLGQHLLELMPFLLIWLLFTLVYKFIPIRKVKFQHAAIGALMAAIFFTLGKKA
                     FIWYITAFPSYQAIYGALAVLPIMLLWIQLSWQVVLLGGQFAAVLDEMETDKGSINDR
                     LNSAG"
     gene            complement(25150..26388)
                     /gene="proA"
                     /locus_tag="PXB27_000415"
     CDS             complement(25150..26388)
                     /gene="proA"
                     /locus_tag="PXB27_000415"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDL5345244.1"
                     /translation="MQMIELAQQAKQASFELAQFGNLQKNQALLTIADSLEQRAEEIL
                     YANAKDIEFAQAQGISLAIIDRLLLNEDRLKDIANDVRNVAKLADPVGQVMDGGVLDS
                     GLKIERQRVPLGVILTIYEARPNVTIDVASLCLKTGNAVILRGGKETKFTNAVLVDVV
                     QDALEKAGLPKLAVQAVTDPDRALLLELLKLDRYIDMVIPRGGAGLHQFCKENSTIPV
                     IVGGIGVCHLFVEKTADQEKSLEVIANAKTQRPSTCNTLETLLVEKAIAVEFLPKLVA
                     KMQQLGVTIHSNDLQKIEGIEPLDQAKLSQEWLSLDLNVVVVNDLQQAVEHIRHYGSQ
                     HSEGILTSDYQLARQFVQQVDAAAVYINASTRFTDGGEFGLGAEVAVSTQKLHARGPM
                     GLEALTTYKWVCEGDYLVRG"
     gene            26672..28555
                     /gene="mutL"
                     /locus_tag="PXB27_000416"
     CDS             26672..28555
                     /gene="mutL"
                     /locus_tag="PXB27_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDL5345245.1"
                     /translation="MINTTPKQLIHILPPQLANQIAAGEVVERPASVVKELVENSLDA
                     GANTIQIDIEKGGSQLIRIRDNGCGISKQDLPLALARHATSKISSLEDLEAILSLGFR
                     GEALASISSVSRLTLTSRTAEQTEAWQAYAQGREMAVEIQPASHPIGTTIEVANLFFN
                     TPARRKFLRTDKTEFAHIDEVVRRIALAKPNISFSLSHNGKIVRQYRKVQDNSVEQQQ
                     KRVAAICGDEFIQHSTYIDRQHGDLHLHGWVGSPALARLQNDLCYSYVNGRMMRDKTI
                     NHAIRQAYGDSIASGYYPAFVIFLDLDPTMVDVNVHPAKHEVRFHQGRLVHDFILQGV
                     LNSLQQTQPTLELRNEVNEPLPSYVQDTNRQAAGQNIFVSQTAPQAAEFTQKNASRSS
                     YTPKFERSNSVSSSAQKWYKELVGGDLHQTTPTIKTQSTLVEPKTEPQTEHKTEAVSQ
                     KITQIAPLASHHSQALAIVKNQAVLIKEEENFYLLPLATLAELKLKLQLEKGQSQALL
                     IPLNLSLDQQQTERWQQTKEELTKLGFSISEKQWQAQTRLTVLSVPQAVREQNLQQLL
                     FALFNQTQAVNLAEFFAKKCEKPTACTLSDVIALLSEIELYANGKKELEGIRKEVDFS
                     KFL"
     gene            complement(28574..28843)
                     /locus_tag="PXB27_000417"
     CDS             complement(28574..28843)
                     /locus_tag="PXB27_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HDL5345246.1"
                     /translation="MREIEYSNAFKRDYKKCAVESLSSSLIEALYFLINDKPLPEKYC
                     DHALTGEWKGFRDCHIQPDLVLIYRKVEDDKLELLRIGSHSHLFG"
     gene            complement(28830..29096)
                     /locus_tag="PXB27_000418"
     CDS             complement(28830..29096)
                     /locus_tag="PXB27_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDL5345247.1"
                     /translation="MANSALVSVRISPEIKEQASEVLAGIGLTVSDVMRMTLAKIATE
                     KRFQFDYQPNFETAEVLRAVKNGEEKLHSANSIADLMEQLNARD"
BASE COUNT         8794 a         6285 c         6132 g         8304 t
ORIGIN      
        1 ataaacaaaa caactttcat atttttgaaa aatttggcat tttttctagt ttttgggcat
       61 tccctgtttt atttttaact cttgaacaac agactaccaa tgattctaga tatctaaacc
      121 gcattagtga aatgttacta aactggattt tgtctccggc attaattatc tatactgcaa
      181 ttatttatgc ttatgtaatt tttattgcgg ctaaggggca aatgccagat ggggttgttg
      241 caaacgtagc gcttccctat ttagctttcg gcctagctat acaagcggtg caattattac
      301 taaaaaatgc aaaatggcag tggttttatt ctcactttac ctatttagca cttttgccgt
      361 taacattaat ttggtatgcc atctatattc gtttcagcca ttatggctta actgaagccc
      421 gtatttattt agtgattggc acaataacct tatctatttg ctatacctta ttactattta
      481 aacagttggc gcaatatcgt ctcttttctg cagtgtcgat agcaattatt ttaatttcag
      541 tgtttatttt agatccgaaa acagttgcgc ttaaagatca aaccaagcgg ttggacaact
      601 atttgctaaa acacaaatta ttagatgtaa ataacaaaat cagcacaaaa gctgtactta
      661 aataccaaga atcattaaat gaaaaccgag atgaagccga atatttttat tcaatgctcc
      721 actatgttgc cagaggaatg cctgctgcac aatttaaaga gaaatatggc gtaaattcaa
      781 gctacgattt aatgagtgaa ataagagaaa gaaatactga cccacgttat tttgaagcta
      841 ctaattctga gctggttcgc ttaactaaag cagatatggc aaatgctcgt gaatatatta
      901 catttagtag ccaattatat cggcctaatt cattttccca tcaaaaaata gaaaataagc
      961 tatgccaaaa actcctttct caaggttact attttgaggg aaatatatta aatattgagt
     1021 atcaatactc agaaacggct caagactgct cttcaataac acaagatcct actgaatttg
     1081 ttatctactt taaaggactt gggactacta ttgaatttaa cattaacgat attttgaaaa
     1141 aaatctttgc taaacatcag ttatctatta cccagcaaca caaaaatgag gttttagaaa
     1201 agattaaagg cgatttactc aaaatggatt taggaaattc agtacttggg cttaagcata
     1261 ttgagttaag atttgaagac aatatcggct atatagtaga aagtattagt acacaatata
     1321 ttatgctgaa ataaaaggag atgctcgttc aattaaattt gaacgagcat ttttcgttaa
     1381 aaactcaatt taagcagcca ctcaaaatta gtctcattta accgatattg cggtttcaaa
     1441 ttcgggccgc aagagtttga gccaatgccg ctcattttgt aatcgacaca cagcacggtg
     1501 gaagggcttt ctaccaaatc ataactgtgg cttttagtgg ttagctcttc ctgtgtgtaa
     1561 ggcgaaagat taaaactaaa cggctgtgga gaagtaacag ttatcccatt tgcttgcaca
     1621 taatgcgtac cgaaatggct gccgttttcc tgcggtttga gataatcggt atgattttct
     1681 cgggcgctgg tttgataagc tccgagtttc gccaaatggt atttgtcgat ataactctcc
     1741 tgctcgccat agccaaaata gctcacttgg ttattccgtt tattcaagaa aaaccgcaag
     1801 ccaaagcgag gtaaaaacgg caaatgagcg gggcgggttg cctgaacctg aatcgtcatt
     1861 tcgccattcg gggcaagatg ataattcact ttcagggtta aaatccgact ttgagcggtt
     1921 gccacaatgc cacattcggc agtaatgttt acgccgtttt tatcttgcat cagttcggta
     1981 tgatacgccc tggtgtaaca gttatcgtag ccggcttttt tccaaaactc acgaattaag
     2041 cggtcgttat cggttggggc tcgccaaata ttgaaatcca acggttgctc aattactgcc
     2101 tcgccgtttt tctcaatccg gctgaaaatg cctttgagtt tatcgaaagt gtagcgatag
     2161 tcgccctgtt gaatccgcca ttcatttgca aaatcttgca caaaaatgac cgcttgcggg
     2221 cgtggattaa gtgctggagt gaccatcgct tgatgcagcg tgatttggtc aaagcctaag
     2281 tgatgccctt tttccaacag agccgtgctt tcatttaaag aataatcaag ggttaaaagt
     2341 tgcaatttgc cgtttggttc gggcagcgtt agcggcaaac aagcggtctg atttggctga
     2401 caatttacca aacattgtcc ctgcagggtg gtttcgccgt tttcgctgaa acagtaatgc
     2461 acggtcagat aatcagctaa gtcgctgaaa tccaactgat ttttcagcca aattttgccc
     2521 gcttttaact cagctcgcac cgggcgattc acatttttca cttctaacaa attagagtgt
     2581 ggcacacgct gttgggaaac caagccgtcc atacagaaat tgccatcgtg caaggtttcg
     2641 ccgaaatcac cgccatagcc gtatttttcg gtcttctgtt cggcacttag ctcagagttg
     2701 gggagcagag gagcatggtc gcaccattcc cacacaaagc caccgcacgc accgctgtag
     2761 cgttggaagg tttgccaata atcttccata tcaccactcg aattgcccat cgcgtgagaa
     2821 tattcacaca aaataaaagg tttgtggttg gcagagtcgg aaaaatattc ctctaaccgc
     2881 tcggtgcttg ggtacatctc gctgtataaa tcgaggtttg aaagatcgtt ttgatgtgcc
     2941 gaatgttggt aaatcgaacc ttcgtagtga gttaaacggc ttggatcccg ttgttttacc
     3001 caacgggctg cgacttcaaa attttcacca aagccggatt cgttaccaag tgaccaaata
     3061 atgaccgaag tgcggttttt gtctcgctcc acattggcaa tgttgcgatc taaaatcgcc
     3121 tgtttgaaca gcggatctct tgcaaaataa cagaaattat cgaccgcttg ttggcgaatt
     3181 tcttcctgtt tgttcgggtt tggcacattc aggaaaatgc tgttttccgg actttcaata
     3241 tacactgcac tggagccgtg agattcaaga tcactttcgg caatcagata aaaaccgagc
     3301 tgatcgcaca attcactaaa ccacggagcg ttcgggtaat gggcggtgcg aatagcgttg
     3361 atgttatggg ctttcattaa gcgtaaatcc accaacgctt gctcacggct aatgctgtag
     3421 ccggttttcg ggtcggaatc gtggcggttt acccctttaa atttaatcgg ggcgttgttg
     3481 aatagcatca cgccattttc cacacgaatc tgacgaaagc cgattttttg ctcaataatt
     3541 tcgttttggt agctcaagca tagcgtgtag agtgtcggat cttccgcatt ccacaaacac
     3601 aaattgcaaa tttcttggca aaaatcaccg cttgtttgct cgcagactaa gcggttttca
     3661 gggtcgaaaa gttgaaaatg cacagcttcg gtgtaggtgg aaaattgtgt ttccaccttg
     3721 agttctgcct ttgaaagatc agcgtttaac tggtagcgga taaagaaatc ttgcaggtag
     3781 ttttgctcac gttccaaaat ataaacatca cggaaaatgc ctgacattcg gaatttgtct
     3841 tgatcttcca aataactgcc gtcacaccat tttagcacca ccacgacgag gctgtttttg
     3901 ccctgtttaa gctgtttggt gatgtcaaat tcattggtgc tgtggctgat ttgcccgtag
     3961 ccgacaaatt cgccgtttac ataaacaaac aggcacgaat ctacgccctc aaaatttagc
     4021 aaatagcgtt tgttaggctt ggggttaaac gtgatttcac gctgatacac cccgcaaggg
     4081 ttttggtgtg gcacataagg cggatcaaac ggaatcgggt agttgatgtt agtgtattga
     4141 tgggcatcaa agccttgagt ttgccagtta gacggcacgg ggatttggtg ggtaaatttg
     4201 gtgttgagga aatcctccgg cagatcgtgc ggacttggat agaaattaaa cttccaatcg
     4261 ccatttagca aagtaaataa ctcagattgt tcccgttcca actgattcgg cgtttgccct
     4321 ttagcaaaag gaataaaata agcgtggtgt agcgtggtat taaggtgcaa cacactcggg
     4381 tcttggtagt aattcggtaa cgacataaaa gccctatgat caaaaagata taaatacgaa
     4441 tacactttaa ttctcttata aaatagggca atcaactatg tgaaaaattg tgaggcggat
     4501 cacaaaataa tttcctcacc tttttccttc gattccgtta taatcacaag aattttttat
     4561 gtcatttttc aaggaattta ccaatgttat atccaagcga agaattttta tatgcaccgg
     4621 ttgagtgggt tgatcacagc gaaggttaca ccgatattcg ttaccataaa tcaaaagacg
     4681 gtatcgctaa aatcaccatt aatcgtccgg aagtgcgtaa tgctttccgt ccgcaaacgg
     4741 taaaagagat gattgaagcc ttttctgatg cccgttttga tgaaaaaatc ggcgtcatcg
     4801 tattaaccgg tgaaggcgaa tatgcgttct gtgccggcgg cgaccaaaaa gtgcgtggcg
     4861 attacggcgg ctacaaagat gaaagcggtg tgcatcactt aaatgtgttg gatttccaac
     4921 gtgatattcg tacctgcccg aaaccggtgg tggcaatggt tgccggctat gcgattggtg
     4981 gtggacacgt tttacatatg ttatgtgatt taaccattgc cgctgacaat gcaaaattcg
     5041 gtcaaaccgg cccgaaagtg gggtcatttg acggcggttg gggtgcaagc tatatggcac
     5101 gtttagtcgg tcagaaaaaa gcgcgtgaaa tttggttctt atgccgtcaa tataatgcac
     5161 aagaagcgtt agatatgggc ttaatcaata ccgttgtgcc ttatgctgat ttagaaaaag
     5221 agactgtgcg ttggtgtcgc gaaatgctac aaaacagccc gattgcctta cgttgcttaa
     5281 aagcagcatt gaatgcagat tgtgacggtc aggctggcct gcaagagctt gctggtaatg
     5341 caacaatgtt gttctatatg acagaagaag ggcaagaagg acgcaatgcg ttcaacgaga
     5401 aacgtgagcc ggacttcagc aaattccgcc gcaatccata attgacggca aatacaagcg
     5461 gttcgatttt gcaaaaaatt tgcaaaaatc gaccgcttac cttttctaat ttaagttaat
     5521 tttatgtcag atgttgagtt aaacgagcag agcgagcaag agcctgagca aaaaccgaaa
     5581 aagcgaaact atttcctttc aaccttattc aaaatcgggg tgataggagc ttgcagtatg
     5641 gctttttacg gcatttattt agacggtaaa atccgtagca aaatggatgg gcagatatgg
     5701 gaattaccag ctgaggtgta tgctcgtatt gaaagcatta aaattgaaga caacttaacg
     5761 cttgagcaaa ccaaacaagc gttgctggat aacggctatc gtcaagtttc tcaagttgcg
     5821 acaccaggcg atttcaaaat tgaagaaaat cagctggtgt tgctacgccg agctttcccg
     5881 tttccagaaa caccggaatc gcaacgagtg cttcgtttaa ctttcaaaca agataaatta
     5941 acttatattg aagatttggt gcagggtaag ttgatcgagg agttccgctt ggatccgaaa
     6001 ttactggcgt tactgcattc cgatagcgat gaagaacgtc aagcattacg cttacaacaa
     6061 taccctcgct tgttaattga gtcactgatt ttaaccgaag atcgccgctt ttatcagcac
     6121 gatgggatta gcccaatcgg tattgcccgt gctttaatca ctaactacca agctggcaga
     6181 acagtgcagg gtggcagtac cttaacgcaa cagttagtga aaaacttgtt cttaagtaat
     6241 gaaagatcgt tggtgcgtaa ggtaaatgaa gcgttaatgt cggtgatttt agacttccgt
     6301 tacgataaaa accgcatttt agaaacctat ctcaacgaaa tttatttagg gcagaacggt
     6361 agctaccaaa ttcacggttt tgcattggca agccaattct attttggtcg tccgatccaa
     6421 gagattagct tagaccaaat tgcgttattg gtgggaatgg taaaaggtcc atcactgtat
     6481 aacccgtggc gaaatccgca aggtgcggta gagcgacgta atgtggtgtt gcgattattg
     6541 ctggatcatc aagtgatcaa tcaagaactt tacgctctgt taagctcccg cccgcttaat
     6601 attcgtgaaa aaggcacgat ttaccgtgag caacctgcct ttatgcaggc attaaccatt
     6661 gatttgaaaa atgagttagg cgaaattaag tcagctcaac tttccggtac taaaattttt
     6721 acgacccttg accgtaaaca gcaacgttca gccgaacaag cagtgattac cggtttggaa
     6781 gatctggaaa gtaaaaataa aaaagtcagc gatttgcaat cggctattgt cgtggcggaa
     6841 taccgcacag gtaaagtgag ggcgatagta ggagaccgct taacccaatt tgccggcttt
     6901 aaccgtgcga ttcagtcaaa acgccaaatc ggttctttag tcaaaccatc ggtttatgcg
     6961 attgccttgg cacagcctga attattccgt ttaaatacac cgatccaaaa taaacaaatt
     7021 tcaattaagg ttggttcaac ctactggaca ccccgtaact acgaccgcag ttttagtggc
     7081 tcggtgatgt taatggacgc cttagtccgc tctctgaata ttccaacggt gaatattggt
     7141 atgaaagttg gcttaaaaaa agtcattgcc aaacaaaaag agatgggctg ggataatgcg
     7201 gaaattccgc cttacccatc aaccttgcta ggttcatatt cgatctcgcc ttacgatgta
     7261 acccgctctt atcaagtaat tgccaacgaa gggctgaaaa caccattaac caccattgag
     7321 gcgattattt cgcacaatgg cgaaatgttc tatcagcgag atagcggcaa agtgagcgag
     7381 caggtgcttc caccggaagc tgcggttcaa acaatgtatg cgatgcaaca ggttgtggaa
     7441 cgaggtacgg ctcgtagctt acaaaacgat tttgccaaac tgaaattagc cggcaaaacc
     7501 ggtacaacca ataacgctcg agatacttgg tttgtgggaa ttgatggcga acacgtcaca
     7561 accgtctggc tgggtaaaga taacaatggg gataccaacc tcaccggttc aagtggtgca
     7621 ttgtatgtgt ataagcaata tcttgagaga gccttgcctc aggcgtatag attaccgaaa
     7681 cttaatacga tgcaatgggt ggggattaat agctatggta gctggaattg cgatagtggc
     7741 agacaaattc cagtttggcg agataaaggg caacgttatt gtaccagtgc aggtaatgta
     7801 gtggctacag aaaaaccaag tgtttgggat gcactttcgc tgaaaaaaga aaatatcgag
     7861 gctaaagcag aagaagctga agggaagcaa ccaatcccta ctgaagaagt tgcaccaatt
     7921 gaataaaaat aaatttgata gccattatca ttcaagatac acaagctgat agattctacc
     7981 tataaaaagt atatattata accatttgct agttttcatt ttggattgta atatagtaat
     8041 aaattaaata agcagtatct aatttaattt tttaagatta acaattaaga ggagcatatt
     8101 atggcaatgt caaatatcgg tttagataaa gaaatcgcaa aaaaattagc agatgagtta
     8161 aatggtttat tagctactta ccaagtgttt tacactaatg tacgtggcta ccattggaat
     8221 gtacgtggtg tgaacttctt tgaattacat gcaaaatttg aagaaatcta cgatgattta
     8281 gtgacaaaag tagatgaaat tgcagaacgt attttaacct taggtcacac acctcaaaat
     8341 gcgttcagcc aatatttaac caagtcacaa attcaagaac atattggtgt ttcagctgca
     8401 caagaatgtt tacgtggtac tttaaatggt ttcaaaacat tactgggaca acaacgtgaa
     8461 attcttgctc tagcaaatga tgcaaacgat gaaggtactg cttctcaaat gagtgattac
     8521 atcaaagaac aagaaaaatt agtatggatg ttcactgcag cttgtgctga atgttcagac
     8581 tacgcacaag gttgttcttg taactaagtt gcaataatat aattaacccc cgataatgaa
     8641 agattatcgg gggttacttt ttcagatagc tatgtcgcaa ggatttcaat ttaaacagtt
     8701 ttttattcgg cacgacagat gtgcaatgaa agtcaataca gatggtattc tattgggagc
     8761 gattgctcac ataaaagatt gtcagaatat tttagatctg ggtataggtt ctggtttagt
     8821 agcacttatg cttgctcaga gaacagatga ttgttgtcac attactgcgt tggaacttga
     8881 accaaatgcc taccaacaag cggtcgaaaa tgcccaaaat tctgcatggg caaatcggat
     8941 ttccgttttg caaggtgatg tgatgcagca ggcatttgaa cagaaatttg acttgattgt
     9001 tagtaaccca ccttattttg cagattcact tgcagctcga accacagaga gagatttagc
     9061 tcgctcaatt agccattaca gccacttgaa ttggctggca aaagcaaaac catggttagc
     9121 agagaacggg aaaattacac tgattttacc tatagcagct gcagaaaaat tagttgacca
     9181 aagtgaacaa attggattgt attgtgttga acaatggtta atttttacca agagcggcaa
     9241 attggctaaa cgcagtatag tgagttttag tttaaacaag caaaagcgtg aacataaaga
     9301 gctgatcatc tatgatctag ataattgtta cactactgag tttaaaagac ttacccaaga
     9361 tttttatttg aatttttaat ttactccccg ggttagtgtt ttccacaaat cttcttcagc
     9421 tacgatgatc tagccttaag ctaaaaagca gattaccacc caagataatc cgctttttag
     9481 acttagctat tatgccaaac ttgctaccat taccgcttta atagtgtgca ttcgattttc
     9541 tgcttgctcg aatgccacat tcattggcga ttcaaacacc tcttcggtta cttcaatgcc
     9601 gtttgccagt gccggatatt tttcggcgat ctctttaccg actttagttt cactgttgtg
     9661 gaacgccggt aagcagtgca taaatttcac tttcgggttg tcggttgctt gcattaattc
     9721 aggcgttact cggtaaggca ttaataaatc tatacgttca gtccagctct ctagcggttc
     9781 acccattgaa acccaaacat cagtatgcac aaaatcgaca cctttaattg cagtctcaat
     9841 atcttcggtt accgtcattc ttgcccccga ttcagctgca aatttttcac acattttcac
     9901 gaatgcatct tccggtaata aggctttcgg ggcacaaatt cgcacgtcca tccccaattt
     9961 cgccccaatc aacaggagtg aattacccat attattgcga gcatcgccga tatagacata
    10021 actgatttgg ctaagcggtt tttcgcaatt ttcgatcatc gttagcacgt cagccaacat
    10081 ttgggttggg tggaattcat cggttaagcc gttaaacacc ggcacgccgg cataatcggc
    10141 aagctcttgt actaccgatt gtttaaagcc gcgatattga atcgcatcat acattctgcc
    10201 tagcactcgg gcggtatctt tcattgattc tttatgcccg atttgcgagg agttcgggtc
    10261 aatataggta acgtgagccc cttgatcgta agctgctact tcaaaggcac aacgagtacg
    10321 ggtcgaggtt ttctcaaaaa ttaaggcaat atttttgccc tttaattttt gctgctcagt
    10381 gccggcatat ttcgcccgtt ttaaatctct tgctaaatcc aacaaaaagc gaatttcacg
    10441 ctcagtgtgg tttactaaac tgagtaaatg tctgtttttt aagttaaatg ccattttgtt
    10501 tctccctgta aacattaaaa tttattttat gcaaaataat tgcataaaaa ttcacttata
    10561 gcaaacagaa tttgcttacc ctcattcggg taaataaaac tcgcctttgc cgactaaaat
    10621 aacctctccg ccaacaaaaa tcttcccgtt ttgatcgact tttaataaca ggcgattcgg
    10681 ccgttgaatt tcatcacctt gtgagatctg aagctggatt ggtattttgc cttgtgcaat
    10741 gtgccaaccg cctaaatttg ccgctgccga accggtgccg gggtcttcga caatcgaacc
    10801 ttgcgattga aaaaacagtc gcactttcgc ctcatttccc tcacgacacc aaacatatac
    10861 actgtctttc aaaaatgctt tttcagccag atttgcattt attttgcact tttcgaccgc
    10921 ttgttgagaa gctaactcaa ccaataactg ctccacgccg gtgtttacat aacaaggctc
    10981 ggacgcaata tccgcaacat tcagccccaa tattgaggca cattctgcac ggcttagcgg
    11041 tgtgttttcc aggttcacac cattggttaa agcaaaagta gtcacattat gttgatgttg
    11101 aatcgctacc aatccggcat tagtttgcaa gcgataggtt gttggaaaat caaaggcgga
    11161 atgcaacaca aaagccgctc caacagttgg gtggccggcg aacggcattt cataatcagg
    11221 cgtaaaaatt ttgagttttt tgaccgcttg tgaatcggtg gaagattgaa taaataccac
    11281 ttcagaaagg ttaaattggc gagcaataag ttgcatttgt aagtcggtta atccgtccgc
    11341 ttcataaaat actgcaagcg ggttgccgcc aaaatggctt gttgcaaaca cattcaccag
    11401 caaataagga tagctctgca tttatcactc cttacataaa cttaaagcca cattgggcga
    11461 aaattctacc attttggtgg cgaatcgtaa aattggaaaa tttatcggta tccctcttta
    11521 gagttaacta tttttgattt atctcaaagt tttaaaaaaa agattaggtc atacttcttt
    11581 ctagtgatta ttttttaatt tgaggttctt atgctttatg cagaattttt acttttatta
    11641 gcctttttat atgcaggtag ccgttacggt ggcatcggtt taggtgtggt ttccggcatc
    11701 ggcttatttg ttgaagtgtt cttcttagga atgccgcttt ccgctccgcc aattagcgtg
    11761 atgctcgtga ttttggctgt agttacttgt gcctccattc tagaagccgc tggcggtttg
    11821 aaatttatgt tgcaagttgc cgaacgcatt ttgcgtagca acccaaaacg tattaccttt
    11881 cttggcccac ttgtcactta tctgatgacc ttaatgctcg gcacagggca ttcggtttac
    11941 tctattatgc cgattatcgg tgatattgcc ttaaaaaata aaattcgccc ggaacgcccg
    12001 atggcagcag cctctgttgc ttcacaatta ggtattaccg gtagcccact ttctgctgca
    12061 gttgcctatt acttaaccga aatcgttaaa ttaccgggct ttgaaggggt gactttattg
    12121 aatgtagtcg gcgtaaccat caccgcaacc ttctgtggtg tgattgcgat gtcattatat
    12181 agcttacgtc gtggtaaaga gttggaagac gatcctgagt atcaacgccg tatgcaagac
    12241 cctgagctac gcaaacaaat cgagcaaacg agtgcaacgt ctttagacga aaaactaccg
    12301 gcaagtgcga aaaactcggt ttatctgttc ctagccgcta ttgcaaccat tgtgattatt
    12361 gcaatgattc cggctattaa accaacggtt gctgctacag gaaaagttgc cggtatggat
    12421 caaatcattc aaatcgtgat gctaactttc gggggtttaa tcttaatttt aactaaaacc
    12481 gacccgaaaa aagtaccaaa cggcatcgtt ttcaaatccg gtatggtagc aaccattgcg
    12541 attttcggga ttgcttggat gagtgatact tacttcaccc atgcaatgcc agcgttcaaa
    12601 gaaggggtaa cagaaatggt tcgagcccaa ccttggacat tcgccttcgc cttgtttgcg
    12661 gtatctgtag tagtaaacag ccaagccgta accgcaaaaa tgttattacc ggtaggcatt
    12721 gcaatgggct tgcctgctcc attattagtt ggtttaatgc cggcaactta tgcttacttc
    12781 tttattccga attatccgtc tgatatcgca accgttaact ttgacgtaac cggtacaact
    12841 aaaatcggta aatattactt caaccacagc tttatggctc cgggtttagt tggcgtaatt
    12901 actgcctgta ttgtcggtat cgccgttgca aatgtgctaa tttaatttta acaaaaaaac
    12961 aagcggtcag attaagtaaa ttttttacca aatctgaccg cttgattatt tactgcaaaa
    13021 ccaattaggc tttgttaaag agttttgcat ctaacgagta ttttccgcca ccgataaaaa
    13081 tataggttaa gaataatacc gaatacacgc ctgctaattc accgccgttt aataaaggta
    13141 atagcacatt gccttgagct ccgtgagcaa taaagaacgc gtatgccatc atacctgaaa
    13201 ggataaacgc tgtttggcgg gtaaatagac ctaaaataaa gaaaattccg cccacaattt
    13261 ccaaaatacc gcctacacca taaatggagg caaggggtac actgccgttt ccgttggtca
    13321 tcgacattgg aaattcaaaa aatttcgctg tgccgtgtaa taaaaacata taaccggcaa
    13381 caacacgggt gattaaaaac gctaaaggtt gtaaatttgt taaagtagag ttcatttgct
    13441 ttccttaaaa taaattgtga tataaaggtc aattgattga ggcaggcatt ctattttaaa
    13501 aacctatact tcacaatcca gccgtcatga aatagattat ttaccattaa taaacaatta
    13561 ggctctcgct aacacccaaa cctgaatttc tactactcca gctttgagta actcggcaca
    13621 aattacattt aaggtggaac ctgtggtgag cacatcatca acaatcgcca ctcgtttata
    13681 ggcttttaac ggttgataga aaaatgcccc acgcagattt ttccgccgtt cagcagcggt
    13741 caattctctt tgtgagacgg ttgctctcac acgttgtaaa ctctgggtat ccagcggaat
    13801 ctgtaaccac ttcgataacc aatcacacaa taattcgctt tgattaaacc ctcttttcca
    13861 atgtctgtgc caaaaaagtg gtacaggcat aatcacttca ggcaagctca gaacctgttc
    13921 cctttgagct tgtttgattg ccagcagcaa ttgtcgggca agggcttgat cccaccaata
    13981 ttgctgctgg aacttaaatt gatgtaccca atgtgaaagt ggcgctcggt attcatttac
    14041 cctcactaat ttatgccact tcggttcatt tttcaaacaa tagccacaag cgtttcgaaa
    14101 ctccaccgca ctcattccac aatgacgaca ataaggggtt ggttttagca atttttggca
    14161 atggctgcaa atgccttgca cgccaatagc gagtggatga ttacaatgaa agcaacggaa
    14221 gccgaataaa ttcacatttc ctccttttat ttgcagatta tcaaatttag ccttattttt
    14281 tccggctatt tttgtgacat cgctcgaaaa aagagtaaaa tctcattatc agtaaataaa
    14341 aaggaaaccg agatgaaatc cctccaaaaa ccgatcaaaa ttgtcttttt cgatattgat
    14401 gacaccctct atgtaaaaca taaagcctat attccaaaat caattaccga gcaggtatta
    14461 cctcgtttaa aagccaaagg cattattccg gccattgcca ccggcagaaa tcacggctgt
    14521 tttcctgaag ctctcaaacc ttatttaaac cctgaaatcg gttttgagct gtttgttacc
    14581 attaacgggc aatataattt ctataaagat caaataatta gtgattattc gttaagccgt
    14641 gagcaaattg aacgtattat cgaaaaagcg aatgctttgg gtattctgta tgcctttatt
    14701 acgccaatga cgattgcagt ttccgaaacc aatccgattg tgcacgaagc ggttttaccg
    14761 atcacaccga attatttgat cgatccacac tactacgaaa aagagaaaat tgtgcaaatg
    14821 ttactgttct acaccgaaga gcagaaacaa gcggtcgaaa atagtggcat ttttgcaaat
    14881 gatttaaaag aggctcgctg gcaccccaat gcggtagata ttctgcgaaa agaaaattcg
    14941 aaagctcgtg gcattcaaga tgtattagca accttgaata ttgagattga aagtgcgatg
    15001 gcatttggcg atggtattaa cgacttggaa atgctcccga ccgtaggtgt tggtgtggca
    15061 atgggtaatg gcgtgccgga attaaaagcg gtggcagatt tcatcaccaa gccgcttgag
    15121 gaagacggca ttctatacgc tcttgaaact cttgaggtga tttaaaagca agcggttgta
    15181 agtttttctc catttacaac cgcttgtagt ttctaattac gcctaaatag cttatcgcct
    15241 gcaatcgcta aaatcgcccc actgacgacc accaacgccc caagataact gatccaattc
    15301 atatccggct tggcaaaaat atgcggataa aaatagtagc taaggttgga aaagatcatc
    15361 gtaaaaatcg gcaacatcgt ggttacaatg ctaacctttg aagtatccca atgattcaac
    15421 gcctcaccgt atgaaccgta gccaattaag gtgttcaaca gcaataaata aagcaacccc
    15481 acagaaaacc atcctcaaga ttgcctaact gtgccggtga ggcaaacggt aacatcagca
    15541 gagaacagcc gatgtaaata ataaacagca cttgctgaga gttaaatttc gctaataata
    15601 atttttgagc cacgccgtat agcacccaaa tcaagctggc tccggcactc aataacactc
    15661 cgagagcgta ggtgttgagc tgtaaaattt cagcaaattt atcgttaaaa aatgcgacaa
    15721 gccctgcaat caataacaca aatccaatct tttgaaacaa gccgaacttt tccttaaaca
    15781 ataatacgcc tagtagaatc atcgaaaatg gagctaactg ccacaatact tggttaatgg
    15841 tgggcgaaac atagtgtaga gcctctgcaa acaaaaagaa atttgccgac agaccacaca
    15901 cccctaacaa aattaaaccg agttcttttt tgctacaagc ggtcaatttg ggtaaacgtt
    15961 ttgcaaatcc tagaatcagc aatagcccta ctgatgccac aacgaaccta acccaaacta
    16021 aagtttgggc atctatcact ttcaatactt gctgggcggc aatcggtagc gttccccagg
    16081 tcattgctgc ggttaatgca agtgaaaagc caagtaaggg gcgttgtttc atcgtggaaa
    16141 ttcctttttc tattctatgt ttctatattt tagaaagatt gagatacaaa aaaaccccgc
    16201 cattggcgag gtctcttatt gattttgaaa tgattagtca gcaatgatgc ttacatattt
    16261 acggcttttc tcgcctttca cttcaaattt aactttaccg tcagcagttg caaataaagt
    16321 gtggtcttta cccataccta catttgaacc tgcgtggaat ttagtaccac gttgacgaac
    16381 gataatgcta cctgctaata cagattcacc accgaaacgt ttaacaccaa ggcgtttagc
    16441 ttcagaatca cgaccgttac gagttgaacc accagctttt ttagttgcca tcttctagat
    16501 ctcctctgaa attatgcttg aatcccagtg attttcactt ctgtgaacca ctgacgatga
    16561 ccttgttgtt tacggctgtg tttacgacga cggaacttaa cgattttaac tttctcgcca
    16621 cgaccttgtg ctacaacttc agccactact tttgcacctg ctacaaccgg tgcaccgatt
    16681 ttaacatctt caccgttaac gaccattaac actgagtcaa attcaacttt ctcaccagtt
    16741 gcaagttcaa gtttttctaa acgaactact tgaccttcac ttactcggtg ttgtttgccg
    16801 ccactttgga aaactgcgta catatttact ccgctatgtg ccaactgctt ttgcttttag
    16861 gcactaaatt aattcataaa aatagggttg caattctacg gaaaaagatt cgaaagagca
    16921 agagcgattt tgagaatttt ttgaaatttg caaaaaatgg taaaaatact accgcttgtt
    16981 tcaactgcga ctaaaaaaca atagactgct ccgattttac aacaaaatca taattctttt
    17041 ttgaacacag gctaaattaa ggtagaatct actcaatttt cgtcttactt tatggattcg
    17101 atggctgtgc aacttttttc tttagaacaa attcaagccc ttgcgaatga agatatgcaa
    17161 gcggtaaatg ccgaaatttt agctcaactt aattccgaag tgctactgat taaccagctc
    17221 ggacattata tcatcgcagg cggtgggaaa cgtattcgcc cgttaattgc ggtgcttgcg
    17281 gctcgtgcct tgggttatcg tggtaaaaag catatcacgg cggctgcttt tattgagttt
    17341 gtgcatactg ctacactttt gcacgatgat gtagtcgatg aatccgatat gcgtcgtggc
    17401 agaccaaccg caaacgccca ttttggcaat gcggctagtg tattggtagg ggatttcatc
    17461 tatacccgtt cgttccagat gatgacggat ttagactctc tgcgtgtgtt aaaagtgatg
    17521 tctgccgcca ccaatgcgtt ggcagaaggg gaagttcagc aattaatgaa cgtgaacgac
    17581 ccaaacacca ctgaagcgaa ctatatgcgg gtcatttaca ataaaaccgc ccgcttgttt
    17641 gaagccgcca ctcaatgcgt ggcgattgcg gctgatgctt ctgaggaaat tgaaaccgca
    17701 ttaaaaaatt acggttgcta cttaggcacg gcattccagc tgattgacga tattttagac
    17761 tactccgctc aaagcgaaag tttaggcaaa aacgtaggcg atgactttgt ggaaggtaag
    17821 ccaaccttgc cattgctcca cgcaatgcac gctgccgaca aagcaggcaa aactactcaa
    17881 gcagagctga ttcgcaatgc tatcgaaaac ggcggcgaat tggaacaaat tccacaagta
    17941 ttggcaatta tgcaagaaca tcaatctctg gattatgcca tgcaaaaagc ccgtgaagaa
    18001 gcccaaaaag cggtggattc attaggggtt ttaccggaaa gcgagtacaa acaagccttg
    18061 gtttcattgg cgtatttatc ggtggatcgc tcttattaat ttgcaaaaaa tcatataaat
    18121 ttaaccactt gtaggggcaa atgatatttg ccccataacc tataattctt ttgggcaaat
    18181 gttatttgcc ccaacataac aatgactgaa caaaaccaac gaccccaaga acatattgaa
    18241 agcgtacgag cgagacgccg tgctcaaaat cagaaaaaag gcccgaatgc cccgtttgtg
    18301 cgtgagaaat taacgctgcc aaacggacat aacaaattac tgctgcactc ttgctgtgcc
    18361 ccttgctcgg gtgaggtaat ggaggctatt cacgcctccg gcattgagtt tacgatctat
    18421 ttttataacc cgaacattca cccgctgaaa gagtatttga tccgcaagga agagaatatt
    18481 cgctttgccg aaaagtgggg tattccgttt atcgatttag acgatgacta cgaaaacgac
    18541 cgcaaagagt ggttcaaaaa agccaaagga atggaatggg agccggaacg aggcattcgt
    18601 tgcacaatgt gctttgatat gcggtttgaa aaagcagcgg aatacgctca caacaacggt
    18661 ttccctgtat ttaccagttg cttaggcatt tcccgatgga aagatatgaa ccaaatcaac
    18721 gactgtggtc accgtgcagc agccaaattt gatgacgtgg tttactggga ctacaactgg
    18781 cgtaaaggcg gcggctcaca acgaatgatc gaaatcagca aacgtgaacg tttctaccag
    18841 caagaatact gcggttgcgt ctattcgtta agagatagca acaaatggcg gttggaaaca
    18901 gggcgtcagc ggattgaaat cggtaagctc tattactcac ccgattaaat gttacttttc
    18961 tttgcttcgc caaagaaaag taaccaaaag aaaggcgacc cgaacttcgc ttagttacga
    19021 tttccttaac ggaaaatttg taaactcgct acgctcaaac agtacaaatt ttcctagaaa
    19081 tcgcaagtcg ctcagacggg acaagcggta cattattcaa aattttttac aaaatatagt
    19141 gatgtactct tcccttctga accgcccgaa cgtgtttgaa ttggtaggaa aatttactct
    19201 gagcgaagcg aatgtaaatt ttccgtgaaa ccaattcaaa ctaagtgagg ataagcggtg
    19261 aagtaggggt gtttctttgg ttactttctt tgcacaagca aagaaagtaa cgaaaactca
    19321 gatatagatt tcaacttaaa aatttacaga aggaataata acaaatgtca aaattcgcta
    19381 tggttttccc cggtcaaggc tcacaagcgg ttggaatgct tgccgacctt gcgccacaat
    19441 atccaatcgt ggaagaaacc ttcaaacaag caagtgatgt gcttggctat gacttatggg
    19501 atttagtgca aaacggtact gccgaagagt tagggcaaac ccaccgcact caacctgcgt
    19561 tgcttgcctc aagcgtggca cttttccgca tttggcaaca aaaattccct gagcaaaaac
    19621 cagccgtgat ggcagggcat agtttgggtg aatactcagc gttggtgtgt gccggtgtgt
    19681 tggatttcca agatgcgatt aaattggtcg agttgcgtgg tcgtgcaatg caagaagccg
    19741 tgccggcagg cagcggtgcg atgtttgcga ttatcggttt agataacgaa agcattatta
    19801 aagcgtgcga gcaagcagca agcgagaccg gtgaaattgt ctctgcggta aactttaaca
    19861 gcccggggca agttgtgatt gccggcacaa aagcagcggc agaaaaagca ggagaattgt
    19921 gtaaagccgc aggtgcaaaa cgtgcgttac cattagcggt gagcgtacct tcacactgtg
    19981 ctttaatgaa accggcggca gaaaaattag ccgaagcctt gcaaaatatt gagctaaatc
    20041 aaccgcttgt ccctgttatc aacaacgtgg atgtggcagt ggaaacagat gccgatacca
    20101 ttcgtaatgc acttgttcgc caactttaca gccctgttcg ttggacggaa accgttgaaa
    20161 aaatggcaca agatggcatc actactttat atgaaatcgg gccaaacaaa gtcctaaccg
    20221 gtttagcagg tcgtattgta aaagaattat cagccaaagc ggtaaatgat gttgccagct
    20281 ttgaggcggt tgaggcttaa ttttcgggag cgtaaaatga gtaaagaaac cctatttatg
    20341 ctaataagtg cattgattgc cttagtgatt ttacctttag cttatactgt tggtatcgct
    20401 aaagaaacat taggcggaaa tatgggaatg ttatccattg ctgccggttg ctcattttta
    20461 gcctattttt taagtctaaa cccgaaaatt cgccacgcct ttatcggagg ttcagctatt
    20521 acagctgctg ctattcaagc ttgggtatta tatggtgtgc tattaagcga aaatgacccc
    20581 aaaagtgcgg tcgttttatt tcaaatttat ctgtttagta gtttagggcc actgtttttt
    20641 tcagctattt gggcatttac tcgcccacgt tggatttacc ctaaacaagg tagtgcggtg
    20701 aaatatgcct tatttggtgg cttaacgacc gtgataaact acttgattac cctttttatt
    20761 ttttatattc tgacgttata acgtcatact aaggagaatt tatgagcaaa atcgcattag
    20821 ttacaggggc aacccgtgga atcggcaaag cgattgccga agaattggta gcaaaaggct
    20881 acatcgtagt cggcaccgca acctctgaaa aaggggcgga atctatttca gcttatttag
    20941 gcgagaacgg caaaggctta gtgctaaatg ttgccgatgc agagtcgatt gagagcgtat
    21001 taaagcagat caaagagacc tttggcgatg tggacgtgtt agtcaataac gccggcatta
    21061 cccgtgacgg cttattaatg cgtatgaaag atgaggattg gttcgacatc attcaaacta
    21121 acttaacttc ggtttatcgt ttatccaaag cggtattacg cccgatgatg aaaaaaggcg
    21181 gacgcattat cacgatcggt tctgtggtcg gctcaatggg caacccgggg caaaccaact
    21241 actgtgctgc caaagcaggc ttagttggtt tttctaaatc tttagcgaaa gaagtagcaa
    21301 gccgcggcat tacggtaaac gtggttgctc cgggctttat cgccactgat atgaccgatg
    21361 agctaaccga agatcagaaa aatgcgattt taagccaaat tcctgccggt gaactcggtt
    21421 ctccacaaga tattgccaaa gcagtcgctt tcttagccag cgatgatgcc cgttatatca
    21481 acggtgaaac cattcacgtt aatggcggtt tatatatgag ctaatccgct acaagcggtc
    21541 ttttttcaac cgagctttgt aaattcagag ttcggttttg ttatttgtta ttttgtgtta
    21601 tcacctgctt tataggtata ggtgaaaaag tgtgaatatg ttatgactag aaatacatta
    21661 gtagtaggct ttttattgtt tgctattttc tttggtgccg gcaatctgat tttcccacca
    21721 aaattaggtt atgaaagtag tacagaattt ttctcagcca tttcaggctt tgttttaact
    21781 ggagtagggc ttcctctact cggtattatc gtcagtgcat tttataatgg aggctataaa
    21841 tctgcattag acaaaatcca cccttggttt tctttagcat tcctaacttc tatctattta
    21901 gcgatcggtc cattctttgc cggtccgcgc acggcagcaa ctgcctatga aatggcgata
    21961 atgccgtttt taggggattc cagccaagtg tcaatgttaa tttttacctt aatttacttt
    22021 gcctttaccc tatggttagc gctaaatcca aataaaatgg ttgatcgcat tggtgcaatt
    22081 cttaccccga tcttgcttat aaccatcatt ggattagtta ttcgagccgg ctttatttta
    22141 gataccgatc cacaaatcac tacgcctaat acgaatgaac aatcttactt ttttaaaggt
    22201 atggttgatg gctatctaac tatggatgca cttgcttcgt tagcctattc ggtaattgta
    22261 ttaaatgcta ttcgtgggaa aggggtaagc caagccgcat tacaacgcca aacagtttct
    22321 gccggcttag ttgcagcagt tgctctagct gtaatttata tttcattggg ttggattggt
    22381 aataatatgc caattagtgc aaacacactt tcgatgttat cagaaagaaa attggatcta
    22441 ggtacttaca ttcttaacac tgtgactacc caagccttcg gtgaactcgg acgtagtgtc
    22501 ttaggagtga ttgtttcact tgcctgttta accactgctg tcggtttagc cgtatcagtc
    22561 agtgaatact ttaatgaaat tttcccgaaa atttcatata aagtttatgc tatcttattt
    22621 actgtgatta gttttactgt tgctaaccaa ggattaaaag aagttattag caaatccatt
    22681 ccggtattat tagttctcta cccaatagca atgactgtca ttttaatgtt gctgattaat
    22741 ttagtgatta aacttccgtt gtctgcacaa cgtctatcac tcctattagt gacaattgta
    22801 gcgatagctt cagttgccgg tgtcgggttt accgaaaact tgcctctaaa atcctactcg
    22861 atggaatggc tacctttcgc tatcattgga gctattattg gagcaattat ccacccgctt
    22921 accggtcgaa agcataaata aagtaaaggc agtgagcatt cactgcctct tttttaggag
    22981 agaataatgc ctgaattacc cgaagttgaa accagcgttc gtggtgtatc gccctatttg
    23041 gttgggcaaa ctattgaaca aatcatcgtt cgtcagccaa agttacgttg gacggtgagt
    23101 cctgaattaa gccaaatggc aggggcaaaa attaccgcag tagaaagacg tgccaaatat
    23161 ctgattatcc gcaccgacaa aggcgatatt ttggtgcatc tgggaatgtc cggctcgctc
    23221 gggattttgc aagaacgtca acaaaaagaa attggcaaac acgaccacgt cgatcttatt
    23281 acccaaaacg gcacaatttt acgctataac gaccctcgca aattcggctg ttggctatgg
    23341 gcagaaaaag cagaagatca cgaactgttg cgtaaactcg ggcctgaacc gctttcaaac
    23401 gatttcacag cagattactt atttgcaaaa agccgcaaaa aaacgaccgc ttgtaaaacc
    23461 tttattatga ccaatgaagt ggttgttggg gtggggaata tctatgcgtg cgaaagttta
    23521 tttatggcag gcattcaccc tgagcttgca gcacaaaacc tgactaaaaa acagtgcgaa
    23581 cgtttagtaa aaaccatcaa agaagtgctg actaaagcga ttattcaagg cggcacaaca
    23641 ctgaaagatt ttatccaacc tgacggcaaa ccaggctatt ttgctcaagt gttgcaagtg
    23701 tatggaagaa aaggggaaac ctgtaatgac tgcggaaagc cgattgaaac taaagtaatc
    23761 gggcaacgta atacctattt ttgcccgaag tgtcagagat tgccgaagta atctaataat
    23821 aaactctctc cccctgaggg agagagacag cttgttgaaa cgttaagtga aacaagcaga
    23881 gagagggtaa aacctaaacc tgcaaccaaa aggtaacagg cccgtcattt tgtaggctga
    23941 cttgcatatc ggctgcaaat tgcccggttt gggtatgaat cttagtggcg gcttgttgat
    24001 gaaagtaggc atacaaacgt tcggcatcaa ccgggcttgc tccttttgag aaactaggac
    24061 gcaagccttt attggtatcc gccgcaaggg taaactgtga aaccactaac aaactgccac
    24121 ctgcttgctg tacgtttaag ttcattttcc cctcgctatc cgaaaacacc cgataattca
    24181 gcactttttc cagtagctta tctgctttcg cttcatcatc gtctttttcc acaccgagca
    24241 gcaccaacaa accgcttgca atttcgccaa cgacttgccc ttcaacctcc acttttgcga
    24301 atttaacccg ctgaattaag cctatcatta atgctcccct tgtctgtttc catttcgtct
    24361 aaaaccgccg caaattgccc gccaagtaat accacctgcc aacttaactg aatccataac
    24421 agcataatcg gtaacaccgc taatgctcca taaattgcct gataagacgg gaaggcagta
    24481 atataccaaa taaaggcttt cttccctaaa gtaaagaaaa tcgctgccat taatgccccg
    24541 atagcagcgt gttgaaattt tacctttcta attggaataa atttatacac taaggtaaaa
    24601 agtagccaaa ttagcaaaaa cggcatcaat tctaataaat gctgcccgag cgataacgct
    24661 ccatttccat taaaaataga aagcgacatt acataggtgc taatcgcaat gctagcaccg
    24721 gcaagcagcg ggccaaacag caaaatgaga aagtaaagca agaaagagaa ccacatcgga
    24781 cgctgacgat tatcgtgcca aatggtattc aatgcctcat caatactctt aatcagcata
    24841 atagtaacca caaataagcc gataaaactt accagcccca tttgtttggc attactgaca
    24901 aacaactcaa tgtattccga caccatctcg ctcgcactcg gggcaaagtt gtcataaatg
    24961 aacgctttta actgctcgtt tgcttcgtgg aaaatcggaa aaaaggtaaa aatggaaaac
    25021 agcacgacaa taaacggcac tatcgccagc attgtgctat aagtgagata accggctgaa
    25081 atcgcaatgt tattttcatt gtaacgcttg gcaaagagct taataaatct ccacagtcga
    25141 tttaacatct taacctctca ccaaataatc gccttcacac acccatttgt aggtggttaa
    25201 ggcttctaat cccatcgggc ctcgtgcgtg taatttctgc gtgctaactg ccacttctgc
    25261 ccctaaacca aactcaccac catcggtaaa acgggtgctg gcattaatat aaaccgccgc
    25321 ggcatcaacc tgttgtacaa attgacgggc aagttgataa tcggaggtta aaatcccctc
    25381 tgaatgctgc gaaccataat ggcggatatg ctctaccgct tgttgcaggt cattcaccac
    25441 caccacgtta agatcaagcg ataaccactc ttggctcaat tttgcttgat caagcggttc
    25501 aattccttca attttttgca aatcgttact gtgaatagta acgccaagct gttgcatttt
    25561 ggcgaccaat ttcggcaaaa attccaccgc aatcgctttt tccaccaaca gcgtttccag
    25621 cgtattgcaa gtgcttgggc gttgcgtttt ggcattagcg atgacctcta gagatttctc
    25681 ctgatccgct gttttttcca caaacagatg acaaacccca atccccccca caatcaccgg
    25741 aatggtcgaa ttttctttac agaattgatg taaacctgca ccgcctcgag ggatcaccat
    25801 atcaatgtag cgatctagct tcaatagttc caataataag gctcgatccg gatccgtcac
    25861 cgcttgcact gccaatttcg gcaagccggc tttttctaac gcatcttgca ctacatcaac
    25921 caacacagca ttggtaaatt tggtctcttt accgccacgc aaaatcactg cattgccggt
    25981 ttttaggcat aatgatgcca catcaatggt cacattcggg cgagcttcgt aaatggttaa
    26041 aatcaccccg agcggcacac gctggcgttc gattttcaaa ccgctgtcta acacgccacc
    26101 gtccatcacc tgccccaccg gatccgctaa tttggcaaca tttcgcacat cgttagcaat
    26161 gtcttttagc cgatcttcat tcaataataa gcggtcaata atcgccaatg aaatcccttg
    26221 tgcttgagca aattcaatat ctttggcatt agcgtataaa atttcttctg cacgttgctc
    26281 aaggctatcg gcaatcgtga gtaaggcttg gtttttctgt aggttgccaa attgagccag
    26341 ctcaaaacta gcttgtttgg cttgctgggc aagttctatc atctgcataa attaggtctc
    26401 atttaatcaa tattctccat aatctaacac gttcttttat gtgaagaaat atgcaaaagc
    26461 caagaaaatt ggataaaatg ggggaatttt aaaacattac gcaattcccc tctccctgat
    26521 ttttctcctg aacggagaaa aatctatccc tctcctacaa ggggagaggg tagaatttaa
    26581 ctctctcccc ttgtgggaga gagaccgcct acagattcgt tcagaatctg taggcagaga
    26641 gaggggtaaa aacagataaa acttaaacaa aatgataaac actaccccta aacagctaat
    26701 ccacattctt ccgcctcaac tggcaaacca aattgctgcc ggcgaggtgg tggaacgccc
    26761 tgcgtctgtg gtaaaggagc tagtggaaaa cagtttagat gccggagcca atactattca
    26821 aatcgacatt gaaaaaggcg gctctcagct tatccgcatt cgagataacg gttgtggtat
    26881 cagcaagcaa gatttaccgc tggcacttgc tcgccacgct acgagcaaaa tcagcagctt
    26941 ggaagattta gaagccattt taagtttagg ctttcggggc gaagcattgg caagtatcag
    27001 ctcggtttca cgtttaacac tgacctctcg taccgcagag caaaccgaag catggcaagc
    27061 ctatgcacaa gggcgggaaa tggcggtgga aattcagcct gcctcacacc cgattggcac
    27121 aaccattgaa gtagcaaatc tgttttttaa cacaccggca cggcgtaaat ttctacgcac
    27181 cgataaaacc gaatttgccc atattgacga ggtggttcgc cgcattgccc ttgccaagcc
    27241 gaatatcagt ttcagcttaa gccataatgg taaaatcgtc cgccaatatc gcaaagtgca
    27301 agacaattcg gtagagcagc aacaaaaacg ggttgcagcc atttgcggtg atgagtttat
    27361 tcaacattca acttatattg acaggcaaca cggggatctg cacctacacg gctgggttgg
    27421 ctcaccggct cttgcccgtc tgcaaaacga cctttgctac agctatgtga acgggcgaat
    27481 gatgcgggat aaaaccatca atcacgccat tcgccaagcc tacggcgaca gcattgcaag
    27541 tggttactat ccggcttttg tgatcttttt ggatttagat ccgacaatgg ttgatgtaaa
    27601 cgttcacccg gctaaacacg aggtgcgctt tcatcaagga agactggtac acgactttat
    27661 tttgcaaggc gtactcaaca gcctgcaaca aacccaacct acgcttgaac tgcgaaatga
    27721 agtgaacgag ccgttaccga gctatgttca agacactaac cgccaagctg cggggcaaaa
    27781 tatttttgtg tcgcaaaccg ctccacaagc ggccgaattt acgcaaaaaa atgcaagtcg
    27841 atcaagctat acgcctaaat ttgaacgaag caacagtgtg agttcgtccg ctcaaaaatg
    27901 gtataaggaa ttggttggtg gcgatttgca ccaaacaacg ccaactatca aaacgcaaag
    27961 cacgcttgtt gagccaaaaa cagaacccca aactgaacat aaaacagaag ccgtttcgca
    28021 aaaaattacg caaattgcac cgcttgcaag ccaccattca caagccttag cgattgtaaa
    28081 aaaccaagcg gtgttaatta aagaagagga aaatttttac ctgcttccac tggcaacatt
    28141 agccgagctt aaactcaagc tacaactgga aaaagggcaa tctcaagcgt tgttgatccc
    28201 actgaatctc agtttagatc aacaacaaac cgaacgctgg caacaaacca aagaagaact
    28261 aactaagctt ggcttttcga tcagcgaaaa acagtggcaa gcccaaacac ggctcacagt
    28321 gctttccgta ccacaggctg tcagagaaca aaacctgcaa cagcttttat ttgctctgtt
    28381 caaccaaaca caagcggtca atttagccga attttttgca aaaaaatgcg aaaaacccac
    28441 cgcttgtacg ctaagtgacg tgattgctct actctcagaa attgagcttt atgccaatgg
    28501 gaaaaaagag ctggagggaa taaggaagga agtggatttt tccaagtttt tatagtctaa
    28561 agggcaatac cctttagcca aataaatgag agtggctgcc aattcgcagc agctctagct
    28621 tatcatcttc cacttttcga tagataagaa ccaaatcagg ttggatatgg caatccctaa
    28681 agcctttcca ttcgccggtt aaagcgtgat cgcagtattt ctcaggtaat ggcttgtcat
    28741 taatcagaaa atacaacgcc tcaattaatg aagaggataa agactcaacc gcacattttt
    28801 tatagtcacg tttaaaagca ttactatact caatctctcg cattgagttg ctccattaaa
    28861 tccgcaatac tatttgctga atgtagcttt tcctcgccat tcttaacagc ccttaatact
    28921 tctgctgtct caaaattagg ctgataatca aactgaaaac gtttttctgt tgcgattttc
    28981 gctaatgtca ttcgcatcac atcagagaca gtcaagccga taccggctaa cacctcactt
    29041 gcttgctctt taatttcagg gctgattctt acgcttacta atgcactatt tgccatcaaa
    29101 aactcctttt gtatatcaaa tgatatacaa aataacattt aacaaaaaat tgtgcaataa
    29161 aaaagaaatt caaagaaaag aaatgaatag ttaaaattag ctcgttacaa gaaatcattg
    29221 agttactcaa gaaatcaagg taatataaag aagtctgggc ggaacacgaa cccgccccta
    29281 gcatctagcc gcttacaaga acagatagat tagttgaaag agcgtgaaaa tttcaactgc
    29341 tgcacacaga aacccaagtg caactaaaat caatagtagt tctttcattg taagcctcct
    29401 tagtgctact aaggatgatt taggctcagg ctactgcgaa tagccctaac gagcctacca
    29461 gtagcacaat caggattgta acaaaaaagc tgtgaatttc acggcttttt ttgta
//
LOCUS       DANLUQ010000011        28570 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.011, whole genome shotgun sequence.
ACCESSION   DANLUQ010000011 DANLUQ010000000
VERSION     DANLUQ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 28570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28570
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.011"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..263
                     /locus_tag="PXB27_000419"
     CDS             <1..263
                     /locus_tag="PXB27_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HDL5345248.1"
                     /translation="ESLASKNPSLPIKMVEHCVKELLEQLTATLEEGERIEVRGFGSF
                     SLHYRQPRLGRNPKTGESVLLGAKYVPHFKAGKDLKERVDLL"
     gene            300..587
                     /locus_tag="PXB27_000420"
     CDS             300..587
                     /locus_tag="PXB27_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HDL5345249.1"
                     /translation="MIKYILGILIVIAVIIVAVTIGANNDQLITFNYIFAQSELRLST
                     LVAILFGLGLILGWLVTGIFYLKIKFQNVALNRRVKRQAQQITELTTPKAE"
     gene            591..1787
                     /gene="lapB"
                     /locus_tag="PXB27_000421"
     CDS             591..1787
                     /gene="lapB"
                     /locus_tag="PXB27_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HDL5345250.1"
                     /translation="MLELLFLLLPIAALYGWYMGQRSAKKDQETASNKFSREYVTGLN
                     FLLSNQQEKAVDLFLSMLQKQESENHIASESQFEAELTLGNLFRSRGEVDRALRIHQT
                     LVNSPHYSFDQKLLAKQQLAKDFMVAGFYDRAENYYISLVDEPEFAKYSLSQLAVIYQ
                     KTREWKKAINVTEKRLRIEPDADKIPLSHFYCEYAQGVKFDDKAAFIESLNKALEYYP
                     QSTRASIILGDFYLEEGEYLRALSYFEQILVQDPDYISEVLGRIKQSYMALEDLNGYE
                     LFLIRANRVKHNSSVDIALTEFIEEKDGINAAHSKLYQQLSTYPNLITFHRFIRYQAD
                     FAEEGNGKESLVLLHNMVGNQIKRSFQYRCLNCGYQSYRLMWQCPSCNQWEKIKPVQS
                     IEGIIQ"
     gene            1811..2503
                     /gene="pyrF"
                     /locus_tag="PXB27_000422"
     CDS             1811..2503
                     /gene="pyrF"
                     /locus_tag="PXB27_000422"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDL5345251.1"
                     /translation="MNNKVIVALDYETEREALEFVDLVDPSLCRLKVGKEMFTTLGTN
                     FVKQLHERKFDVFLDLKYHDIPNTVARAVRSAADLGVWMVDLHASGGLAMMEEAKKIL
                     EPYGKEAPLLIAVTVLTSMEDLDLLQIGINASPMEQVIRLAHLAQRAGLDGVVCSPQE
                     VEVLRTHCGQDFKLVTPGIRPKGTDFGDQRRVMTPKQAVETGSDFLVIGRPITQAQDP
                     LAVLKSINQSIS"
     gene            2586..2897
                     /gene="yciH"
                     /locus_tag="PXB27_000423"
     CDS             2586..2897
                     /gene="yciH"
                     /locus_tag="PXB27_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HDL5345252.1"
                     /translation="MTLVYSTETGRIKSEVIKEERPKGDGIVRIQRQTSGRKGKGVCL
                     ITGLDLEDKALNSLASELKRKLGCGGSFKDGVIEIQGDNRGFIKQILEQKGFKVKLAG
                     G"
     gene            3000..5162
                     /locus_tag="PXB27_000424"
     CDS             3000..5162
                     /locus_tag="PXB27_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HDL5345253.1"
                     /translation="MWKKTLIAVFLASQYAFAVGTGKEPSKSYTETELKQLKNDWLAI
                     YQKELQYAENLRLKQRENFQQINYLADAAISQKNLSLSTEQIIQRLLLTLAGYPLEMK
                     AEWKLLNAKITLKQATESEVKAFISKYPDSLYQRQLQNLMFELLYREQKFPELLEYAQ
                     NVKQSSLADQCRLFSARYELAASKAQINPEVSQVTEQKAENAELLALVQEFDRFWLAT
                     PKLTSDCANLESYWRDQGLKTDEKMRLKAVELIKQNANAELANLAANVNNENLKIWLS
                     SVEKVANNPADLQNFIENQPLDSPFYADNKAIIQQLFAKYVRTLSENMENPSFQQYQT
                     WAEKYQLSSEEINGWKNAFITRFFDNPEPTFQFWRDEQIKLLKTDNLTERRLRMAIWQ
                     KADLNEWLVLLSQQAKEKAEWRYWAAKEEKNEAKRKVDFESLAKERGFYAMLAAQQLG
                     IVYQIPMLEVPELTQAQLELYANQLARIQELRELGQFDQARKIWIDWIKNIKPEAGEN
                     TPTKIQLALIKYAKDKNWYDLAVEGTIQTKAFDYLDLRLPNAYSDWFDLHLQDKKISK
                     TFAQAIARQESAWNAQAQSHANARGLMQLLPTTAQKTAKDNGLTFKDGNDLFQPFNNI
                     MLGTTHLMELNEKYPNNRILIASAYNAGAGRVEQWLKRSNGQLAMDEFIASIPFYETR
                     GYVQNVLAYDYYYQMLYSDINDKNGLKMFYQEELTRKY"
     gene            5211..5287
                     /locus_tag="PXB27_000425"
     tRNA            5211..5287
                     /locus_tag="PXB27_000425"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5245..5247,aa:Met,seq:cat)
     gene            5514..5975
                     /gene="rimP"
                     /locus_tag="PXB27_000426"
     CDS             5514..5975
                     /gene="rimP"
                     /locus_tag="PXB27_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDL5345254.1"
                     /translation="MATLEQKLEDLVLDSIEALGFELVGIECQRAGRFLTVRLYIDKE
                     GGVTIDDCSDVSRQVSAIFDVEDPIADKYNLEVSSPGLDRPLFTVAHYERFIGQDVVI
                     HLRIPMFDRRKWQGKIKQVEGDFITLTVENEDRQFAFGNIQKANLVPVFNF"
     gene            5996..7492
                     /gene="nusA"
                     /locus_tag="PXB27_000427"
     CDS             5996..7492
                     /gene="nusA"
                     /locus_tag="PXB27_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDL5345255.1"
                     /translation="MSKEILLAAEAVSNEKQLPKEAIFEALETALTIATKKSRDLDID
                     VRVVVDRKTGDFQTFRRWAVVENVNNMTREISLEAAQFEDPNVQLGDFIEDEVESIEF
                     GRVAIQTFGQVVKTKIREAEKTKIIDEFRSDLGKIILATVKKVTRDNIFLELQGKSED
                     IKGEAVIVREDMIPRENFRPGDRVRGVLYEIKPESKGPQLFVTRAKPVMLEELFKLEV
                     PEIGEGVIEIKGSARDVGSRAKIAVKTHDKRIDPVGACVGMRGARVQAISNELGGERV
                     DIVLWDENPAQFVINAMAPADVSSIVINDETHSMDIAVEAKNLAQAIGRNGQNVRLAT
                     QLTGWALNVMTVEELTKKSEEEDGKVINLLTSALEIDEEFAQVLIEEGFTSLEAIAYV
                     PVDTLTAIDGLEDEDLVEELQSRAKNAITKKALEEEKLLKSAQIEEKLLTLEGMDRHI
                     AFKLAEKQITTLEELAEQGVDDLADIEELSEEKAGELIMAARQICWFN"
     gene            7477..10044
                     /gene="infB"
                     /locus_tag="PXB27_000428"
     CDS             7477..10044
                     /gene="infB"
                     /locus_tag="PXB27_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDL5345256.1"
                     /translation="MLVQLSKEDNKMTEEKKTLSLGGARKASKVNTTTTSGKVKAVEV
                     KEKKPKIDAKALQEKAAKEAAAAKARAEKEAAEKLAKEKAEAEAKEKAAKKAAESTAS
                     KPISVPVMPSSKAKEEKPKAQQVQPKQEKAVDPEKEAKRKEEAELRRKQEELAQQKAE
                     MEAKRAAENARRLAEIAREEAEDNGNEDFDDERFTSSYAREAERDNDRRSEGNRGRNG
                     KGNVAKAKKGGREEDKNERSADRRNHKEVKGKGKQGKKGAALQQAFTKPAQVVKADVV
                     IGETITVAELANKMAVKATEIIKMMMKMGEMVTINQVIDQETAQLVAEELGHKVILRK
                     ENELEEAVLEDRDTHAEKVTRAPVVTIMGHVDHGKTSLLDYIRKAKVAAGEAGGITQH
                     IGAYHVETDDGKMITFLDTPGHAAFTSMRARGAKATDIVVLVVAADDGVMPQTIEAIQ
                     HAKAAGAPLVVAVNKIDKPEANPDRVEQELLQHEVISEKFGGDVQFVPVSAKKGMGID
                     DLLEAIILQSEVLELTAVKDGMASGVVIESYLDKGRGPVATILVQSGTLNKGDIVLCG
                     FEYGRVRAMRDENGKDVESAGPSIPVEVLGLSGVPAAGDEATVVRDEKKAREVALYRQ
                     GKFREVKLARQQKAKLENMFSNMAAGDVAELNIIVKADVQGSVEAISQSLMELSTDEV
                     KVKIVGSGVGGITETDATLAAASNAIMVGFNVRADASARRVIENENIDLRYYSIIYEL
                     LNDVKAAMSGMLQPEFKQEIIGLAEVRDVFRHPKFGAIAGCMVTEGIVKRNNPIRVLR
                     DNVVIFEGELESLRRFKDDVSEVRNGMECGIGVKNYNDVKVGDQIEVFEVVEVKRTI"
     gene            10129..10509
                     /gene="rbfA"
                     /locus_tag="PXB27_000429"
     CDS             10129..10509
                     /gene="rbfA"
                     /locus_tag="PXB27_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDL5345257.1"
                     /translation="MSREFKRSDRVAQELQKEVAVILQREVKDPRIGMVTVSDVEVSR
                     DLAYAKIFVTFLFDNDAEAIKQGMQGLEKASPYIRTLLGKAMRLRIVPELRFIYDESL
                     VEGMRMSNLVSSVIKNDEEKHKED"
     gene            10512..11435
                     /gene="truB"
                     /locus_tag="PXB27_000430"
     CDS             10512..11435
                     /gene="truB"
                     /locus_tag="PXB27_000430"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDL5345258.1"
                     /translation="MSRPRKRGRDIHGVFLLDKPQGMSSNDILQKVKRIFQANKAGHT
                     GALDPLATGMLPICLGEATKFSQFLLDSDKRYLVTAKLGERTDTSDAEGQVVETKAVA
                     VTKSQILAALEKFRGDILQVPTMFSALKHNGKPLYEYARAGITVEREARPITIFELNF
                     IEYKAPFLTLEVHCSKGTYIRTLVDDLGEELGCGAHVTMLRRTAVADYPIEKMMSYAD
                     LQNLAENQPLAELDQHLLPMDTAVSALAKINLSSEQTKAVGFGQRIRFDNLNQLYGLV
                     RLYSEDEQFLGVAEITNDTVIRPNRMINLTE"
     gene            11438..12518
                     /locus_tag="PXB27_000431"
                     /pseudo
     CDS             11438..12518
                     /locus_tag="PXB27_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252783.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
     gene            complement(12556..13080)
                     /gene="tadA"
                     /locus_tag="PXB27_000432"
     CDS             complement(12556..13080)
                     /gene="tadA"
                     /locus_tag="PXB27_000432"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HDL5345259.1"
                     /translation="MFIKPNQTSCPLQISEQDIAFMQYALDLADLAEAKGEIPVGAVL
                     VDKNQQIIGRGWNQTIQLCDPSAHAEMQAIRQAGQTLGNYRLLDCTLYVTLEPCPMCA
                     GAILHSRIKRLVFGASDYKTGAVGSRYHLFEDYKMNHFLEVHGNVLGRECSQKISDFF
                     KQRRLQKKGDALIK"
     gene            complement(13162..14970)
                     /locus_tag="PXB27_000433"
     CDS             complement(13162..14970)
                     /locus_tag="PXB27_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL5345260.1"
                     /translation="MTTESLTFADLGLPQPILDAVNEMGFVNPSPIQQECIPHLLAGR
                     DVLGMAQTGSGKTAAFSLPLLAQIDPTQRHPQMLVMAPTRELAIQVADACEQFAKNMK
                     GIRVVTVYGGQRYDIQLRALKQGSQVVVGTPGRILDHIRRGTLDLSELKFIVLDEADE
                     MLRMGFIDDVETVMAELPETHQTALFSATMPEPIRRITRRFMKDPQEVKIKATERSAP
                     DIEQSYWLVNGFRKNDALLRFLEVEEFDAAIIFTRTKTGTIDITELCERNGYRTAALN
                     GDMTQQAREQTLDKLRSGRLDILVATDVAARGIDIERISLVINYDIPLEEESYVHRIG
                     RTGRAGRSGRALLFVEPRERRLLRNIENLIKKPINEVAIPNHEILMEKRREKFKARIS
                     KQLEHHDLEKYRELLEDLFTADQDHEELAAAMMMLLQEKQKLILPPDPVVRAARSERG
                     RDRNERNDRRNGREHRENNGVAMDLYRIELGREDGVEVRHIVGAIANEGDISSRYIGH
                     IKLYDKYSTIELPQGMPNHLVQHFAHKARVLSKPMQMTLLGSANSASNANPFEGRGRR
                     GNDRNDRGGRRDGGFNKDRKGGFKEKRFSEKRGRRD"
     gene            complement(15172..16095)
                     /gene="nlpI"
                     /locus_tag="PXB27_000434"
     CDS             complement(15172..16095)
                     /gene="nlpI"
                     /locus_tag="PXB27_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HDL5345261.1"
                     /translation="MVQLFKLFRIRFLLLNLISVLMLSGCTSRYADLVSIKNLALAEL
                     NPQLRFEQEAMVVRLSQVLEEAPLTPSEQADLYFERGVIYDSLGLWSLARYDFAQAIS
                     LNQRMAAAYNYMGLYLLLEGDYDSSIDAFNAVLELDREYSYTYLNRGLAFYYSGRYSE
                     AQRDLLHFYEEDKKDPYRALWLYFNELEFTPTEAKNNLQKRANNLSSDYWGTNIVNYF
                     LGKLNLAQVRAIMERDAKENSSQYAEILTETYFYLAKQQLKLDHKDEAISLFRLALAN
                     QVFNFVEYRFALFELSQIRGMTQTTPVVDKE"
     gene            complement(16176..18326)
                     /gene="pnp"
                     /locus_tag="PXB27_000435"
     CDS             complement(16176..18326)
                     /gene="pnp"
                     /locus_tag="PXB27_000435"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDL5345262.1"
                     /translation="MTPIVKQFKYGQHTVTLETGAIARQATAAVMASMDDTTVFVTVV
                     AKKDVKEGQDFFPLTVDYQERTYAAGRIPGGFFKREGRPSEGETLIARLIDRPVRPLF
                     PEGFFNEIQVIATVVSVNPQISPDLVAMIGASAALSLSGVPFNGPIGAARVGFINDQF
                     VLNPTTSEQKISRLDLVVSGTDKAVLMVESEADILTEEQMLAAVVFGHEQQQVVIENI
                     KEFVKEAGKPRWDWVAPEPNTDLINKVKALAETRLGDAYRIVEKQVRYEQIDAIKAEV
                     IAQLTAEDETVSEGTIIDIITALESQIVRSRIIAGEPRIDGRTVDTVRALDICTSVLP
                     RTHGSALFTRGETQALAVATLGTERDAQIIDELTGEKSDRFLFHYNFPPYSVGETGRI
                     GSPKRREIGHGRLAKRGVLAVMPTAEEFPYVVRVVSEITESNGSSSMASVCGASLALM
                     DAGVPIKAAVAGIAMGLVKEDEKFVVLSDILGDEDHLGDMDFKVAGTRTGVTALQMDI
                     KIEGITPEIMRIALNQAKGARMHILGVMEQAIPAPRADISDYAPRIHTMKIDPKKIKD
                     VIGKGGATIRALTEETNTSIDIDDDGTVKIAATDGNAAKAVMARIEEIVAEVEVNQIY
                     NGKVTRVVDFGAFVSILGGKEGLVHISQITNERVERVADYLTVGQEVQVKVVEIDRQG
                     RIRLTMKDINNTNEANAEETVAENVVETEQENNF"
     gene            complement(18588..19868)
                     /gene="phoR"
                     /locus_tag="PXB27_000436"
     CDS             complement(18588..19868)
                     /gene="phoR"
                     /locus_tag="PXB27_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HDL5345263.1"
                     /translation="MKVKLSLKHFLLELLLAAAVAGVFNLFFPAYFFWFSFCLLVLLI
                     WHHHTESSLLQLLRPNEQSGRSKVTTMEHLSQSAAFYHERLRQDKIKTLRLLSKLNKH
                     IQYLPDAIIICQENGEISWCNNVAQELFEFYWDKKSNKSIFNVIFYPEFKSYFRQQRH
                     HRPLVLLTHNKRYIELNLNRYDSNSRLIVARDVTSFVRLMHSRQTFLANMNHELRTPL
                     TILQGYLEMLENEPHNMALQQKALNAMKEQSQRMTNLLRQLSLLVKIEHSNDSEHNEV
                     QMSALISAIQKNTEILSQNQQILFDIEPNLTVWGDQEQLQSAVSNLIYNAIRHAGEGV
                     TIQICCQRDNQEPNTAIFSVQDDGIGIDAHHITHLTERFYRVDESRSNKTGGSGLGLA
                     IVKHALEQHHSTLEIESEAGKGSRFSFKLPLIKK"
     gene            complement(19871..20557)
                     /gene="phoB"
                     /locus_tag="PXB27_000437"
     CDS             complement(19871..20557)
                     /gene="phoB"
                     /locus_tag="PXB27_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006996031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HDL5345264.1"
                     /translation="MNNKILIVEDERAIREMLSLYLSKQYQVIEAEDYQSAINKLGEK
                     PELILLDWMMPGRSGIHLIKYLKKQPETEKIPIIMLTARSSEEDCITGLNAGADDYIT
                     KPFSPQVLLARIEAVWRRSYDPPSNLINIDGLILDQQAQRVLVAEQEVKLSGTEYKLL
                     HFLMTHPEKVYRREQLLDFIWGNDIYVEDRTVDSYIRRLRKSLEPYGFERYIQTVRGS
                     GYRFSNHFQD"
     gene            complement(20641..21411)
                     /gene="pstB"
                     /locus_tag="PXB27_000438"
     CDS             complement(20641..21411)
                     /gene="pstB"
                     /locus_tag="PXB27_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HDL5345265.1"
                     /translation="MLPTDLETKLHINNLNFYYGDFHALKNINMRIAKNKVTAFIGPS
                     GCGKSTLLRTLNRMFELYPNQRATGEINLDGENLLTSDTDISIIRAKVGMVFQKPTPF
                     PMSIYDNVAFGIRLFEKLPKSELNDRVEWALTKAALWNEVKHKLNQNGDSLSGGQQQR
                     LCIARGIAVKPEVLLLDEPCSALDPISTMKIEELINELKHDYTVAIVTHNMQQAARCS
                     DYTAYMYLGELIEFGDTKQIFDKPKFRQTEDYIKGKMG"
     gene            complement(21421..22269)
                     /gene="pstA"
                     /locus_tag="PXB27_000439"
     CDS             complement(21421..22269)
                     /gene="pstA"
                     /locus_tag="PXB27_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005650601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDL5345266.1"
                     /translation="MKNYQNRRFYCRKIKNVLMLSLAYSAVLFGLFWLVWIIVTLLQK
                     GIPALSWELFTRPTPAPNETGGLRNAIVGSILIVSTATLIATPIGILAGTYLSEYGRY
                     TKLAKFTRFLNDVLLSAPSIIIGLFIYAIYVAQVKHYSGWAGSLALAVLVIPIVVRTT
                     DSMLNLVPNNLREAAAALGCPRWRVITMISYRAARSGIITGVLLAVARISGETAPLLF
                     TALSNQFASLDMNAPMANLPVVIYQYAASPFADWNTLAWAGAMLITLFVLSLNIITRL
                     YFSKTK"
     gene            complement(22266..23225)
                     /gene="pstC"
                     /locus_tag="PXB27_000440"
     CDS             complement(22266..23225)
                     /gene="pstC"
                     /locus_tag="PXB27_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDL5345267.1"
                     /translation="MHKHKIDNKCLNHPWVEGLFHHTTRFFALLVFALLVVVLVSLLL
                     GSWEALSRFGVSFLWENEWNPVNDRYGALVPIIGTLISAGIALVIAVPVSFGIATFLT
                     ELAPQWLKRPVGTAVEMLAAIPSIIYGMWGLFVFVPLFQEYVQPYLIDTFGQLPFIGM
                     LFSGAPFGIGLFSAGLVLAIMIIPFIASIMRDVFSVVPPLLKESAYGLGSTTWEVIWK
                     VVLPYTKTGVIGAIMLGLGRALGETMAVTFVIGNAFMLPDSVFSPSTSIASAIANEFN
                     EAGGLQKSALMSLGLILFLITSIVLAASRLLIARMGKQEGKRE"
     gene            complement(23306..24343)
                     /gene="pstS"
                     /locus_tag="PXB27_000441"
     CDS             complement(23306..24343)
                     /gene="pstS"
                     /locus_tag="PXB27_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005756281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDL5345268.1"
                     /translation="MAKRFKLLTVLGISFSLSVAANAQTITGAGASFPYPIYAKWAGL
                     YEKETGNKINYQSIGSGGGQQQIIAKTIDFGASDDPMKGETLAEHKLLQFPAIIGGTV
                     PVVNIPELKSGELKLSGKVLADIFLGKITQWNDPAIVALNQDIALPAKTILVVRRSDG
                     SGTTFGWTNYLSKVSAEWKEKVGEGKSVKWPVGQGGKGNEGVAAYVKQLKYSIGYVEY
                     AYAKQNALAWVSMQNADGKFVQPSRETFAAAAANADWSQTAGMGVILTNEAGEKAWPI
                     TAASFILIHQHSDKPEQTKGVFKFFDWAFKHGKDAAAELDYVPLPDNVVEKIQTQWKT
                     EVKDGQGNSLY"
     gene            24528..26774
                     /gene="parC"
                     /locus_tag="PXB27_000442"
     CDS             24528..26774
                     /gene="parC"
                     /locus_tag="PXB27_000442"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDL5345269.1"
                     /translation="MTTEINYEGIEQMPIKRFTEDAYLNYSMYVIMDRALPFIGDGLK
                     PVQRRIIYAMSELGLNASAKYKKSARTVGDVLGKFHPHGDSACYEAMVLMAQPFSYRY
                     PLVDGQGNWGAPDDPKSFAAMRYTESKLSKIAEILLGELGQGTVDYQPNFDGSLEEPK
                     YLPARLPHILLNGTMGIAVGMATDIPPHNINELADASVMLLDNPKATLDDVLSVVQGP
                     DYPTEAEIITPKAEIARMYEQGRGSIKMRAVWKKEEGEIVISALPHQASPSKIIEQVA
                     TQMRNKKLPMVDDIRDESDHENPIRIVIVPRSNRIDFDALMDHLFATTDLEKSYRVNM
                     NMIGLDGKPAVKNLLTILNEWLSFRRTTVTRRLNYRLDKILNRLHILDGLMIAFLNID
                     EVIEIIRNEDEPKAELMARFNLTDVQAEAILNLRLRHLAKLEEHQLQAEKSELEKERD
                     ELQLILGSERRLNSLIKKEIQADAKAFASPRRSPLVERAESKAIAESDLTPTEDVTVI
                     LSEKGWVRCAKGHDIDVQALSHRAGDGYLAHARGRSNQPVVFLDSTGRAYALDPTSLP
                     SARSQGEPLTGKITLPEGAVVQQVLMASPETKVLMASDSGYGFICTFADLVSRNKAGK
                     AVISLTENAKVLPPQLLENDENLSLVAMSNVGRMLVFPVSELPQLSKGKGNKIINISA
                     AAAKSGDEYLARLLVIKPTNSLVFVSGKRKITLKPSDVDNYRGERARKGSQLVRGLST
                     NSTVEIVE"
     gene            complement(26845..28197)
                     /gene="lysC"
                     /locus_tag="PXB27_000443"
     CDS             complement(26845..28197)
                     /gene="lysC"
                     /locus_tag="PXB27_000443"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HDL5345270.1"
                     /translation="MSHLSVAKFGGTSVANFDAMTSSANIVIADANTRVVVLSASAGV
                     TNYLVELANGCEKEHRDEVIAAVRQIQYNIIEKLQHQDAVKAEIDELLERIETLAESA
                     SLATSAALSDELISHGEMMSTKIFTQLLIERGHPAVWVDVRDVVATNSHYGKAAPNDE
                     KTQQQSDNVIKPLIASGKIVITQGFIGRDDEGKTTTLGRGGSDYSAALLAEVLNANDV
                     LIWTDVPGIYTTDPRVVPNAQRIDTMAFNEAAEMATFGAKVLHPATLLPAVRSNIPVY
                     VGSSKAPEQGGTWVTRDPQPRPTFRAIALRRNQILLTLSSLSMLHAQGFLANVFAILA
                     KHKISVDVITTSEVSVALTLDKTGSASSGADMLSAELLDELNAYCNVQVESDLALVAI
                     IGNNLHAQSGVAKKLFHTLENYNIRLISYGASSNNVCTLVKNAEADDVVRSLHKGLFE
                     "
BASE COUNT         8555 a         5588 c         6368 g         8059 t
ORIGIN      
        1 ttgagagttt agcatctaaa aatccaagtt taccaattaa aatggtggag cattgtgtaa
       61 aagagctttt agagcagtta acggctactt tagaagaagg tgaaaggata gaagtaagag
      121 gatttggtag tttttctcta cattatcggc agccgcgttt aggtcgcaat cccaaaacag
      181 gagaaagtgt tcttttaggc gcgaaatatg taccacattt taaagctggt aaagatctaa
      241 aagagagagt tgatctgctt taagtcttat taattcaata atcgtagtaa ggagattata
      301 tgattaagta tattttaggt attttaattg tgattgcagt tattattgtc gctgttacga
      361 ttggagccaa taatgatcaa cttatcacct ttaactatat ttttgctcag agtgaattac
      421 gcttatcaac gttagttgcg attttatttg gcttaggatt gattcttggt tggttggtaa
      481 cgggtatttt ttacttaaaa attaagtttc aaaacgttgc tttaaatcgt cgtgtaaagc
      541 gccaagctca gcaaataact gaattaacga ctccaaaggc tgaataatca atgcttgaat
      601 tattgttcct tttattgcct attgcagcac tatatggctg gtatatggga caacgtagtg
      661 caaagaagga tcaagaaacc gctagtaata agttctctcg tgagtatgtt acggggttaa
      721 attttctttt atctaatcag caggaaaagg cggtggacct tttcctctct atgttacaaa
      781 aacaagaatc cgaaaatcat attgcatctg aatcccaatt tgaagcggag ttaaccctag
      841 gtaatctttt ccgttcaaga ggtgaggtag atcgtgcctt acgtattcac caaacattag
      901 tgaatagccc tcattattca tttgatcaaa aattattagc aaaacagcaa ttagctaaag
      961 actttatggt tgcaggtttc tatgaccgtg cagaaaatta ttacatttca ttagttgatg
     1021 aacctgagtt tgctaaatat tctttatctc agcttgctgt tatttaccaa aaaaccagag
     1081 aatggaaaaa agcgattaat gttacagaaa aacggcttcg aatagagccg gatgcagata
     1141 aaataccgct ttcacatttt tattgtgaat atgcgcaagg ggttaaattt gatgataaag
     1201 cggcatttat tgaatcgtta aataaagcgt tagaatatta tcctcaatcc acacgcgctt
     1261 ctataatatt aggtgatttc tatttagaag agggagaata tctaagagca ttaagctatt
     1321 tcgaacagat tcttgtgcaa gaccctgatt atattagcga agtgcttgga cgaattaagc
     1381 aaagctatat ggctcttgag gacttaaatg gctatgagtt atttctcatt cgagcaaacc
     1441 gagtaaaaca taatagtagt gtagatattg ctttaactga atttattgaa gaaaaagatg
     1501 gtatcaatgc tgcacattct aaactatacc agcagttgag tacttatccg aatttaatta
     1561 cttttcaccg ctttattcgc tatcaagcag attttgcgga ggaaggaaat ggtaaggaaa
     1621 gcttagtttt attacataat atggtgggaa atcagataaa acgcagcttc caatatcgct
     1681 gtttaaattg tggttatcaa agttatcgct taatgtggca atgtccttct tgtaatcaat
     1741 gggaaaaaat taagccggta caaagtatcg aaggtattat tcaataaaga aagaatctga
     1801 ggaattgaaa atgaacaata aagtaattgt tgcgttagac tatgaaactg aacgtgaagc
     1861 attagaattt gtggatttgg tcgatccgtc gctttgtcgt ttaaaagtag gcaaagaaat
     1921 gtttacaacc ttaggcacaa attttgtcaa acaattacac gaacgtaaat ttgatgtatt
     1981 ccttgattta aaatatcatg atattccaaa tacggttgct cgagcagtac gttctgctgc
     2041 tgatcttggt gtgtggatgg ttgacttgca tgcttctggt ggtttagcca tgatggaaga
     2101 ggcaaagaaa attttggagc cttatggcaa agaggcacca ttattaattg cagtgacggt
     2161 tctaaccagt atggaagatt tggatttgct tcaaataggt attaatgctt cgccgatgga
     2221 acaagttatt cgtttagcac atttagcaca acgggctggt ttagatggcg tggtatgttc
     2281 tccacaagaa gttgaggtat tacgtaccca ttgtggtcaa gattttaaat tagtgacccc
     2341 aggaatccgg ccaaaaggaa cagattttgg ggatcaacgc cgagtgatga cacccaaaca
     2401 agcagtagaa acaggctcag attttttggt tattggtcgt cctattaccc aagctcaaga
     2461 tccgcttgcg gtattaaaat ctattaacca atctatcagt taattcattg catttttaat
     2521 gaaggcggaa aaatttccgc cttttttaat caaacaagcg gttatttttg aggaaaaatt
     2581 tgtaaatgac attagtctat tcaacggaaa caggcagaat aaagtccgaa gtaataaaag
     2641 aagaacgccc taaaggggat ggtattgtgc gtattcaacg tcaaacaagc ggtagaaagg
     2701 ggaaaggtgt ttgccttatc accggtcttg atttagagga taaagcctta aattctcttg
     2761 cttcagagct taaacgtaaa ttgggttgtg gtggctcatt taaagatgga gtaattgaaa
     2821 tccagggcga taatcgagga ttcattaagc aaatcttaga acagaaaggt ttcaaagtga
     2881 aactggcagg tggttgagtt ttaaacaatt atcagctggc agtttttaaa taacttcatt
     2941 ttatttgaaa cttcattcct acttttctgc ctaaaactca cgattaagga gaaaaactaa
     3001 tgtggaagaa aacgttgatt gctgtatttt tagcaagcca atatgctttt gcggtaggaa
     3061 ccgggaaaga accatcaaaa agttatactg aaacagaatt aaaacaatta aaaaatgatt
     3121 ggttagcgat ttatcaaaaa gagctacagt atgcagaaaa tttgcgttta aaacagagag
     3181 aaaattttca gcaaattaat tatttggcag atgcagcgat tagtcagaag aatttatcac
     3241 tttcaacgga gcagataatt cagcgtttat tactaacctt agcgggttat ccgttggaga
     3301 tgaaagctga atggaagctg ctaaatgcta aaataacgtt aaaacaagcc acagaaagtg
     3361 aagttaaagc atttatttca aaatatcctg attctttgta tcaaaggcag ttacagaact
     3421 tgatgtttga gctgctctat cgtgaacaga aatttccgga gttactggaa tatgctcaaa
     3481 atgtaaaaca gagtagtctt gccgaccaat gccgtttatt ttctgctcgt tatgaactgg
     3541 cagcaagtaa agctcaaatc aatccggaag tttctcaagt aactgagcaa aaagcagaaa
     3601 atgcagaact gcttgctttg gtgcaagagt ttgatcgctt ttggcttgct acgccgaaat
     3661 taacttctga ttgtgcgaat ttagaaagtt attggcggga tcaggggctt aaaaccgatg
     3721 aaaaaatgcg tttgaaagcg gtcgaattaa ttaaacaaaa tgcaaatgca gaattggcga
     3781 atttggcggc aaatgtaaat aatgagaatt tgaaaatctg gctatcttcc gttgaaaagg
     3841 tagcgaataa tccggcagat ttgcaaaatt ttatcgaaaa tcaaccgctt gattccccat
     3901 tttatgctga taataaagcg attattcagc aactctttgc taaatatgtg cgaactttat
     3961 cggaaaatat ggagaacccg agtttccagc agtatcaaac gtgggcagag aagtatcaac
     4021 tttccagcga ggaaatcaat ggctggaaaa atgcttttat cactcgattt tttgataacc
     4081 cagaaccaac attccaattt tggcgtgatg agcaaatcaa gttgctcaag acagacaatt
     4141 taactgaacg ccgtttacga atggcaattt ggcaaaaagc agatttaaat gagtggctgg
     4201 ttctgctatc tcagcaggcg aaagaaaaag cagaatggcg ttattgggct gcgaaagagg
     4261 agaaaaacga agcgaagcgt aaagttgatt ttgaaagctt agctaaagag agaggctttt
     4321 atgctatgtt ggctgctcaa caattaggca ttgtttacca aatcccgatg cttgaagtgc
     4381 ctgaattgac acaagcacag cttgaattgt atgcaaacca attagctcgt attcaagagt
     4441 tgcgagagct agggcagttt gaccaggcaa gaaaaatatg gattgactgg ataaaaaaca
     4501 tcaagccgga agcaggtgaa aatacaccaa ctaaaatcca gttagccctg ataaaatatg
     4561 ccaaggataa aaattggtat gatttggcgg tggaaggtac aattcaaaca aaagcctttg
     4621 attatcttga tttgcgatta ccgaatgcct attcggattg gttcgattta catctgcaag
     4681 ataaaaaaat cagcaaaacg tttgctcagg cgattgctcg tcaagaaagt gcgtggaatg
     4741 ctcaggctca atcgcacgct aatgcgagag ggctaatgca actgttaccg acaacagcac
     4801 aaaaaaccgc taaagataac ggtttaacct tcaaagatgg caatgatctc tttcaaccgt
     4861 ttaacaatat tatgttaggc acaacccatt taatggagtt aaacgaaaaa tatccgaata
     4921 atcggattct gattgcctct gcttataatg caggagcagg gcgtgttgag cagtggctga
     4981 aacgtagtaa cgggcagtta gcaatggatg aatttattgc cagtatcccg ttttatgaaa
     5041 ccagaggcta tgtacaaaat gtgctggcgt atgactacta ttatcaaatg ctctatagcg
     5101 atataaatga taaaaatgga ttaaaaatgt tctatcaaga ggaattaaca agaaaatatt
     5161 aaaaaatgct tgtgtttttg tgtagaaccg ctataatgca ccccatcaga cgcggggtgg
     5221 agcagcttgg tagctcgtcg ggctcataac ccgaaggtcg ttggttcaaa tccggccccc
     5281 gcaaccagtt ttaagctcac agctttggtt aaagttgtga gttttgtttt atatgctgta
     5341 gcaaatttgc aaaaaatttg gcaaaaaaga ccgcttgttg tggttgctac agcaaaaaac
     5401 aaaccgctta aagccccacg gtttcccgtc tggggctttt ttgcggaaat tggtacagca
     5461 ttaacgtatc aaagctggtt tatccggctt tttttatatt tggagattat gacttggcaa
     5521 ctttagaaca aaaattagaa gatttagtgc tcgattctat tgaagcatta ggctttgaat
     5581 tagtcggcat tgaatgccaa cgtgcaggcc gttttttaac ggtgcgttta tatattgata
     5641 aagaaggtgg cgtgacgatt gatgattgta gcgatgtcag ccgccaagtg agtgcgattt
     5701 ttgatgtgga agatccgatt gccgataaat ataacttaga ggtttcttca ccagggttag
     5761 atcgtccatt atttaccgtt gcccactatg agcgttttat cgggcaagat gtagtgattc
     5821 atttacgcat tccgatgttc gatcgccgta aatggcaagg taaaatcaaa caagtagaag
     5881 gtgatttcat cactttaacg gtagaaaatg aagatcgcca gttcgcattt ggcaatattc
     5941 aaaaagcaaa tttagttccg gtttttaatt tttaagataa aggtaaactg acaaaatgag
     6001 taaagagatt ttattagctg cggaagcagt ttcaaacgaa aagcaattac caaaagaggc
     6061 aatttttgaa gccttagaaa ccgctttaac cattgcaacc aaaaagagcc gtgatttaga
     6121 tattgacgtg cgtgtggtgg tagatcgtaa aaccggtgat ttccaaactt tccgtcgttg
     6181 ggctgtggtt gaaaatgtga ataatatgac ccgtgaaatc agcttagaag cggcacaatt
     6241 tgaagatcca aacgtacaat taggcgattt tattgaagat gaagtagagt ctatcgagtt
     6301 cggtcgtgtg gcgattcaga cttttggtca ggtagtcaaa accaaaattc gtgaagcgga
     6361 gaagacgaaa atcattgatg aattccgttc ggatttaggc aaaattattc ttgccactgt
     6421 gaaaaaagta acccgtgata atattttcct tgagttacaa ggtaaatcag aagatatcaa
     6481 aggcgaagcg gtcattgtgc gtgaagatat gatcccacgt gaaaacttcc gcccgggtga
     6541 ccgtgtgcgt ggtgtgttat acgaaatcaa gcctgaatca aaaggacctc agcttttcgt
     6601 tacccgtgct aaaccggtga tgttagaaga gctattcaaa ttggaagtgc ctgaaattgg
     6661 cgaaggcgta attgagatca aaggttcagc ccgtgatgtc ggctctcgtg caaaaattgc
     6721 cgtgaaaacc cacgataaac gtattgaccc ggtaggggct tgtgtgggta tgcgtggtgc
     6781 tcgtgttcaa gctatcagca atgaattagg cggcgagcgt gtggatatcg tactttggga
     6841 cgaaaatccg gctcaatttg tgattaatgc aatggctccg gcagatgtaa gctctattgt
     6901 gattaacgat gaaactcact caatggatat tgcggttgaa gcgaaaaatc tggctcaagc
     6961 tatcggtcgt aacggtcaaa acgtgcgttt ggcgacacaa ttaactggtt gggcattaaa
     7021 tgtaatgacc gttgaagagc taacgaagaa gagcgaagaa gaagatggta aagtcatcaa
     7081 tttattaact tctgctttag aaattgatga agaatttgct caagttttaa ttgaagaggg
     7141 ctttacgtct cttgaagcga ttgcttatgt gccggttgat actttaacgg cgattgacgg
     7201 tttagaagat gaagatttag tcgaagaatt acaatctcgt gccaaaaatg cgatcacgaa
     7261 aaaagcgtta gaagaagaga agttattaaa atcagctcaa attgaagaaa aattattaac
     7321 gcttgaaggt atggatcgcc atatcgcatt caaattagct gaaaaacaga ttacgactct
     7381 tgaagaactt gcagagcaag gtgttgatga tttagccgat attgaagaac tttcagaaga
     7441 gaaagctggc gagctaatta tggcagcacg ccaaatttgt tggttcaact aagcaaagag
     7501 gataataaaa tgacagaaga gaaaaaaaca ctttcgcttg gtggcgctcg caaagcgagt
     7561 aaagtgaata caaccacaac cagtggtaaa gtaaaagcgg ttgaagtaaa agagaaaaaa
     7621 ccgaaaattg atgcaaaagc attgcaagaa aaagcagcaa aagaagctgc cgcagcgaaa
     7681 gctagagctg aaaaagaagc agctgaaaag ttagccaaag aaaaagccga agctgaagct
     7741 aaagagaaag ctgcaaaaaa agcagcagaa tcgaccgcta gtaaaccgat ttctgtgccg
     7801 gtaatgccaa gcagcaaagc aaaagaagaa aaaccgaaag cacaacaggt acagccaaaa
     7861 caagagaaag cggttgatcc tgaaaaagaa gctaaacgca aagaagaagc tgagttacgc
     7921 cgtaagcaag aggaattagc tcagcaaaaa gcggaaatgg aggcaaaacg tgctgcagaa
     7981 aatgcccgcc gtttagcaga aattgctcgt gaagaagcgg aagataacgg caacgaagat
     8041 tttgatgatg agcgttttac ttcttcttat gctagagagg ctgagcgtga taatgaccgc
     8101 cgtagtgagg gaaatcgtgg tcgtaacggc aaaggcaatg tggctaaagc gaaaaaaggc
     8161 ggacgtgaag aggataaaaa cgaacgcagt gcagatcgtc gcaaccataa agaagtaaaa
     8221 ggcaaaggta aacaaggtaa gaaaggtgca gctttacaac aagcctttac caaaccagct
     8281 caagttgtta aagctgatgt ggtgattggt gaaactatca ctgtggctga attggctaat
     8341 aaaatggcag tgaaagcgac tgaaatcatc aaaatgatga tgaaaatggg tgaaatggtg
     8401 acgatcaacc aagttatcga ccaagaaacc gcacaattag tggcggaaga acttgggcat
     8461 aaagtgattt tgcgtaaaga aaatgagtta gaagaagcgg ttttagaaga tcgtgatact
     8521 cacgcagaaa aagtaacccg tgcaccggta gtgactatta tgggccacgt tgaccacggt
     8581 aaaacctcgt tacttgacta cattcgtaaa gccaaagtgg cggcaggtga agcaggtggt
     8641 attacccagc acattggtgc ataccacgtt gaaaccgatg acggcaagat gatcactttc
     8701 ttagataccc cggggcatgc agcctttacc tcaatgcgtg cgcgtggtgc gaaagcaact
     8761 gacatcgttg ttctcgtcgt tgcggctgat gacggtgtaa tgccacaaac tattgaagct
     8821 atccagcacg ccaaagcagc gggtgcgcca ttagtggtag cggtgaataa aattgataaa
     8881 ccggaagcga accctgaccg tgtagaacaa gagttactac aacacgaggt tatttctgag
     8941 aaattcggtg gcgatgtgca attcgtgccg gtatctgcga aaaaaggtat ggggatcgat
     9001 gatttactag aagccattat tctgcaatcc gaagtgcttg aattaaccgc agtaaaagac
     9061 ggtatggcaa gcggcgtggt aatcgaatct tacttagata aaggtcgtgg ccctgtggca
     9121 actatcttgg ttcaatccgg tacgctaaac aaaggcgaca ttgttctatg tggttttgaa
     9181 tacggacgtg tgcgtgcaat gcgtgatgaa aacggtaaag atgttgaatc agcaggtccg
     9241 tctattccgg tagaagtatt aggcttatca ggcgtgcctg ctgccggtga tgaggcaacc
     9301 gtcgtgcgtg atgagaagaa agcccgagaa gtggctcttt atcgccaagg taaattccgt
     9361 gaagtaaaac tggctcgtca gcaaaaagct aaactggaaa atatgttcag taatatggca
     9421 gccggtgatg tggcagaatt gaacatcatt gttaaagcag acgtacaagg ctcggtagaa
     9481 gcgatcagcc aatccttaat ggaactttca accgatgagg tgaaagtgaa aattgttggt
     9541 tcaggtgtag gtggtattac cgaaactgat gcaaccttag ccgcagcttc taatgctatt
     9601 atggtcggct ttaacgtgcg tgccgatgct tctgcccgcc gtgtgattga aaacgaaaat
     9661 atcgatttac gttactactc catcatttat gagctattaa atgacgtgaa agcagcaatg
     9721 agcggtatgt tacagcctga attcaaacaa gaaattatcg gcttggcaga agtgcgcgat
     9781 gtgttccgcc acccgaaatt tggggcgatt gccggttgta tggtgacaga aggtatcgtt
     9841 aaacgtaaca acccaatccg tgttctgcgt gataacgtag taatttttga aggcgagtta
     9901 gaatctctcc gccgtttcaa agatgacgta tctgaggtgc gtaacggtat ggaatgtggt
     9961 atcggcgtta aaaactacaa cgacgtaaaa gttggcgacc aaatcgaagt gtttgaagtg
    10021 gttgaagtaa aacgtactat ttagcctaat tttgtgtagg ggcgtattgc atacgccctt
    10081 tgtatttttt agggcgtgaa gcatcacgcc cctataaaag agaaaaatat gtccagagaa
    10141 tttaaacgta gtgaccgtgt ggcacaggag ctacaaaaag aagtggcggt gattttacaa
    10201 cgtgaggtaa aagatcctcg cattggtatg gtgactgttt ctgatgtgga agtatcaaga
    10261 gatttggcgt atgccaaaat ttttgtgact ttcttatttg ataacgatgc tgaagctatt
    10321 aagcaaggta tgcaaggctt ggagaaagca agcccctata ttcgaaccct tttaggtaaa
    10381 gcaatgcgtt tgcgtattgt gcctgaactg cgttttatct acgatgaatc gctagtcgaa
    10441 ggtatgcgta tgtctaacct tgtgtctagt gtaatcaaaa atgacgaaga aaaacataaa
    10501 gaggactaat tgtgtctaga cctcgcaaac gtggccgtga tattcacggc gttttcttat
    10561 tagataaacc acaaggaatg tcttcaaacg atattctgca aaaggtaaag cgaattttcc
    10621 aagcaaataa agccggacat accggggcgt tagaccctct cgcaaccgga atgctgccga
    10681 tctgtttagg tgaagcgacc aaattttcgc aatttttgtt ggattcggat aaacgttatc
    10741 tcgtcaccgc aaaattaggc gagcgaaccg atacttcaga tgcggaaggg caagtggtgg
    10801 aaaccaaagc agttgcagtg acaaaatctc aaattttggc tgctttggag aaatttagag
    10861 gtgatatttt gcaagtgccg acaatgtttt cggccttaaa acacaacggc aaacccttgt
    10921 atgaatatgc ccgagcaggg attacggtgg agcgtgaggc tcgcccgatt acgatttttg
    10981 aactaaattt cattgaatac aaggctccat ttttaaccct tgaggtgcat tgttctaaag
    11041 gtacttatat ccgaactttg gtcgatgatt taggcgaaga gttgggctgt ggtgcacacg
    11101 ttacgatgct acgccgtaca gcagtggcag attatccgat tgaaaaaatg atgagctatg
    11161 cagatttgca aaatttagcc gaaaatcaac cgcttgcaga gttagaccag cacttattgc
    11221 cgatggatac ggcagtttct gcattagcaa aaataaattt aagttcagag caaactaaag
    11281 cggtgggttt tggtcagaga attagatttg ataatctgaa ccaactttat ggtttggtac
    11341 gtttatattc agaagatgaa caatttctcg gtgtggcaga aatcaccaat gatactgtga
    11401 ttcgtcctaa ccgaatgatt aacttaacgg aataagaatg aaatcgataa tttttacctt
    11461 acctttagcg attatcactt tagccttagc cggttgttca agtgataatc aagaaagtta
    11521 tgccggctta aacttagatg ctaaatacag taaatctcgc tcggtcagtc attttaatga
    11581 ttatgtgcat tttctgaaac agaaggcagc aggggcagga gtgtctgcaa aaaccttaaa
    11641 tgctcagaaa ttcattcatt acaacgccag agcggtgcag ttagatcaac agcaagctgc
    11701 acgaagacgt gacccgaatg taccgcctcc gccaccaaat ccaagtttta aacaaagtat
    11761 taactcaagc gaaggtggat caggcaagtg aacgttggta tgaatataat agccagctta
    11821 cccatgccag ccaaaaatat ggggtacaaa aagagtatat tttggcactt tggggaatgg
    11881 agagcagctt tgggcgtaac caaggggatt ttgatgtact ttcggtgttg gcaaccctcg
    11941 cctttgatgg tcgccgtgag aagttattta cgcaagaatt tgtgaatgcg atgaaaatgc
    12001 tcgatgacgg cacaattaaa cgctatgaat tgcttggatc ttgggcagga gcgatgggac
    12061 aaactcaatt tatgccaact tcttacttaa cctatgcagc tgatggcaat aatgacggta
    12121 aaaaagatat ttgggagaat gaagccgatg catttgcctc gattgccaat tatctttcaa
    12181 cagtaggttg ggataatcaa ctaccttggg gtgttgaggt taaattagct tcaccgatgg
    12241 atttaagttt cgccggtctt gaaaccaaca aagcgaaaac attggcggaa tggcaagcct
    12301 taggtattta tttagcttat ccgaatggac aagaatcggc aaaattagct caattacacg
    12361 gcaaagaggt ttggttgatt cgtccggata aagaagcggg aagagcgttt ttagtttcta
    12421 ataatttccg tactttaatg gattggaata aatcgaataa ccttggtatc agtatcggaa
    12481 aatttgccga tcgaatttta gcttcagttg gtcaataatc gaataaaagc atagtagcaa
    12541 agcctatcta tgcttttatt ttatcaaagc gtcgcctttc ttctgcaaac gacgttgctt
    12601 aaaaaaatca ctgatctttt ggctacattc tctgcccaat acattgccat gcacttctaa
    12661 gaaatggttc attttataat cttcaaacag atgatagcgt gaacctaccg cacctgtttt
    12721 ataatcactt gccccaaaaa ccaatcgctt aatccgacta tgtaaaattg caccggcaca
    12781 cattgggcaa ggctcgagcg taacataaag ggtacaatct aacagacggt aattccctaa
    12841 agtttgtcct gcttgacgaa ttgcctgcat ttcagcgtgt gcagatggat cacagagttg
    12901 aatggtttgg ttccagcctc tgccgataat ctgttggttt ttatcaacca gtactgcccc
    12961 aaccgggatt tcgccttttg cttcggctaa atcagcgaga tcaagggcgt attgcataaa
    13021 ggcgatgtct tgctcggaga tctgaagtgg acaagaagtt tgatttggtt tgataaacat
    13081 agttgcaaaa aattgagttt ttttgaccgc ttgtaaaatg ccccacgagg gtggggcatc
    13141 gcttacttcg cttagtgtcg tttagtcacg acgaccacgt ttttcactga aacgtttttc
    13201 tttaaagcca cctttgcggt ctttattgaa acccccgtct cggcgaccac cacgatcatt
    13261 acgatcgtta ccacggcgac cacgaccttc aaatggattt gcattacttg cactgtttgc
    13321 agagccaagt aatgtcatct gcattggttt gcttaataca cgtgctttgt gagcgaaatg
    13381 ttgtacgaga tgatttggca ttccttgcgg caactcaatg gttgagtatt tatcataaag
    13441 tttgatatgc ccgatgtaac ggctgctgat atccccctcg ttggcaatcg ctccaacaat
    13501 atgacgaact tccacaccgt cttcacgccc caattcaatg cggtataaat ccattgccac
    13561 accattgttt tcacggtgtt cacgaccgtt acgacgatcg tttcgctcat tgcggtcacg
    13621 tccacgttca gaacgggcag cacgcacaac cggatccggt ggcagaatca gtttttgttt
    13681 ttcttgcagt aacatcatca tcgccgctgc taattcttcg tggtcttgat ccgcagtaaa
    13741 taaatcttcc aacaattcac gatatttttc taaatcgtga tgttctaact gcttagaaat
    13801 gcgggctttg aatttctcac ggcgtttttc cattaaaatt tcgtggtttg gaatcgccac
    13861 ttcattaatt ggttttttga ttaaattttc gatattgcgt aataagcggc gttcacgagg
    13921 ctcaacaaac agtaaggcac gacctgaacg accggcacga ccggtacgtc cgatgcggtg
    13981 aacataagat tcttcttcta acggaatatc gtagttaata accaagctga ttcgctcaat
    14041 atcaatacca cgggcagcaa catcggtggc gactaaaata tctaaacgac cggagcgtaa
    14101 tttatctaag gtttgctcac gggcttgctg ggtcatatcg ccgtttaatg ccgcagtacg
    14161 gtagccgttg cgttcacaca attcggtaat atcaatagta ccggttttgg tgcgggtaaa
    14221 gatgatagca gcatcaaatt cttctacttc taggaaacgc aataacgcat cgtttttacg
    14281 gaagccgttt accagccagt agctttgttc aatatccggg gctgaacgct cggttgcctt
    14341 aatttttacc tcttgcggat ctttcataaa acgacgggta atacggcgaa tcggctccgg
    14401 catggtggca gagaagagag cagtttgatg tgtttccggt aattcagcca ttaccgtttc
    14461 aacatcgtca ataaagccca tacgcagcat ttcatcggct tcatctaaca cgatgaattt
    14521 taattcggat aaatctaatg tgccacggcg gatatggtca agaatacgcc caggtgtacc
    14581 cactactact tgtgagcctt gttttaaagc acgtaattga atgtcgtaac gttgtccacc
    14641 ataaaccgtc actacacgaa tgcctttcat attttttgca aactgctcac aagcatcggc
    14701 tacttgaatc gcaagctcac gggtgggtgc catcaccaac atttgcgggt ggcgttgtgt
    14761 tgggtcaatt tgtgccaaca gcggcagcga gaatgcggcg gttttaccgc tcccggtttg
    14821 tgccatacca agcacatcac gaccggctaa taaatgagga atacattctt gttgaatggg
    14881 tgatgggttt acaaagccca tttcatttac agcatcaagg attggttgag gtaagcctaa
    14941 atcggcgaaa gttaaagatt cagttgtcat aaaaaaccat ttgtttggaa cgcacaaagc
    15001 agaaaatatc gactattttt ctgcacagcc attaatgaaa attatattgt tgaaatttag
    15061 gaagaaatga aataacaaag caagtgatgc tttaaaaatc ccctgagtat aagcggttaa
    15121 atttacaaaa ttttttgcga aattaaccgc ttgtattgtc ctaatctttc cctattcttt
    15181 atcaacaacg ggagtcgttt gagtcatacc acgaatttgt gataactcaa acagggcaaa
    15241 acgatactca acgaagttaa acacttgatt tgcaagagca agacgaaaga ggctgattgc
    15301 ctcatcttta tgatctaatt tgagttgttg ttttgctaag tagaaatacg tttctgtcag
    15361 tatttctgca tattgtgagc tattttcctt agcgtcacgt tccattatag cacgaacttg
    15421 agctaaattg agcttcccta aaaaataatt tacgatattt gttccccagt aatccgaaga
    15481 aagattatta gctcgctttt gtaaattatt tttagcttct gttggtgtaa attccagctc
    15541 attaaaatag agccataatg ctcgataagg atctttctta tcttcttcat aaaaatggag
    15601 aagatcacgc tgggcttcag aatatctgcc gctataatag aaagccaaac ctcgattgag
    15661 ataggtgtag ctatattccc gatctaattc caatacggca ttaaacgcat caatcgaact
    15721 gtcgtaatcg ccttccagca gcagatacag ccccatatag ttataagctg ccgccatacg
    15781 ttgattgagc gaaatggctt gagcaaaatc ataacgggct aacgaccata aacctaaact
    15841 gtcgtaaata acgcctcttt caaaatagag gtctgcttgc tcagagggcg ttaatggggc
    15901 ttcttccaat acttggctga gtcttaccac cattgcctct tgttcaaagc gaagttgagg
    15961 attaagttct gccaatgcta agtttttaat tgaaaccaaa tcggcatacc gactggtaca
    16021 gccgcttagc attaaaacag aaattaaatt cagtaataag aaacggattc gaaacaactt
    16081 aaaaagttgt accattacag catatccctc aattaaggaa aattaaaaac tgaataacgg
    16141 acactcaaag tccgttattc gcctttatta ttgaactaga aattattttc ttgttctgtt
    16201 tctaccacat tttcagctac agtttcttct gcatttgcct cgttggtatt attgatgtct
    16261 ttcatcgtca gacgaatgcg tccttgacgg tcaatttcta ccactttcac ttgtacttct
    16321 tgaccaacgg ttaagtagtc cgctacacgc tcaacacgtt cgttggtgat ttgtgaaatg
    16381 tggactaaac cttctttgcc acctaagatg gaaacgaatg caccgaagtc caccacacgg
    16441 gttactttac cgttgtagat ttggtttact tccacttcgg caacgatctc ttcaatacga
    16501 gccattactg ctttcgctgc attgccgtca gttgccgcaa ttttcaccgt accgtcatca
    16561 tcaatgtcga tagaagtatt ggtctcttcg gttaaagcac gaattgttgc accgccttta
    16621 ccaatcacat ctttgatttt cttcggatcg atcttcattg tgtgaatgcg tggggcatag
    16681 tcagaaatat ctgcacgagg tgccggaatg gcttgttcca ttacacctaa aatgtgcatt
    16741 cttgcacctt tagcttggtt taaggcaata cgcataattt caggggtgat cccttcgatt
    16801 ttgatgtcca tttgcagcgc agtcacaccg gtacgggttc ccgctacttt aaagtccata
    16861 tcgcctaagt ggtcttcatc acctaagatg tcagaaagta ccacgaattt ctcgtcttct
    16921 ttcacgagcc ccattgcgat accggcaacc gctgctttga ttggcacacc tgcgtccatc
    16981 aacgcaagag acgcaccaca cacagatgcc attgaagaag aaccgttaga ttcagtgatt
    17041 tcagacacca cacgcactac atacgggaac tcttcagcgg ttggcatcac ggctaatacg
    17101 ccacgttttg ctaaacgacc gtgaccgatt tcacggcgtt ttggcgagcc gatacgaccg
    17161 gtttcgccca cagagtatgg agggaaattg tagtggaata agaaacggtc agatttttcg
    17221 ccggtcaatt cgtcaatgat ttgggcatca cgctctgtgc ccaatgttgc tactgctaat
    17281 gcttgggttt cgccacgggt gaaaagagca gaaccgtggg tgcgtggtaa cacactggtg
    17341 caaatatcca atgcacgcac ggtatccacc gtacggccgt caatgcgtgg ttcgcctgca
    17401 ataatacggc tacgcacgat ttggctctct aatgcggtga tgatgtcgat gatagtccct
    17461 tcagaaacag tttcatcttc tgcggtaagt tgtgcaatca cctctgcttt aatcgcatcg
    17521 atttgctcgt aacgaacttg tttttctacg atacgataag catcgccaag gcgtgtttct
    17581 gctaatgctt ttactttgtt gattaaatct gtatttggct ctggtgcaac ccaatcccaa
    17641 cgtggtttgc ccgcttcttt aacaaattct ttgatgtttt cgattacaac ctgttgttgc
    17701 tcgtggccga acaccaccgc cgctaacatt tgctcttcgg ttaagatatc cgcttcagat
    17761 tccaccatca acacggcttt gtctgtacct gaaaccacta aatctaagcg gctgattttt
    17821 tgctcgctgg tggttgggtt taatacgaat tgatcgttga taaaaccgac acgagccgca
    17881 ccgattggac cgttaaacgg cacgccggat aatgaaaggg cagccgatgc accgatcatc
    17941 gcaaccagat caggactgat ttgtgggttt accgatacta cggtcgcaat cacttgaatt
    18001 tcgttaaaga aaccttctgg gaaaagtgga cgcacaggac ggtcgatcaa gcgagcgatt
    18061 aaggtttcac cttcgctagg acgtccttca cgtttgaaga aaccgcccgg aatacgaccg
    18121 gctgcgtaag tacgctcttg ataatctacg gttaatggga agaaatcttg cccttctttt
    18181 acgtcttttt tcgctactac ggtaacaaat acggttgtgt cgtccatact tgccattact
    18241 gctgccgttg cttggcgtgc gatagcaccg gtttctaagg tcacggtgtg ctgaccgtat
    18301 ttaaactgtt ttacaattgg agtcattgaa attttccttt taaagaattt actattattg
    18361 tcgttttatt tctctaaatt gcgactagca aaaaggcttt taagtaaaac atttttgata
    18421 gccgcctctc aataacaaga aataaaactt gtctagtata gcgtgatttt tataaaaaat
    18481 cgcctattat tttgatcttg cacctcagtt ataaagaaat tcaaaacttc agtgtaaaat
    18541 ggggattagt ttgtgaaaag ccttgtgaaa atagtttgat tgcatcgcta tttttttatt
    18601 agaggtagtt taaaactaaa tctgcttcct ttaccggctt cactttcaat ttccagtgtg
    18661 gaatggtgct gttcaagagc gtgtttgaca atagccagac ctaaaccgct accgccggtt
    18721 ttgttgctac gagattcgtc cacacgatag aaacgttctg tcagatgggt aatatgatgg
    18781 gcatcgatac caataccgtc atcttgcacg ctgaaaatcg cagtattcgg ctcttgatta
    18841 tcacgttgac aacatatttg aatggtaact ccttcaccgg cgtgccgtat tgcattgtaa
    18901 attaaatttg atacagcgct ttggagttgc tcttgatctc cccataccgt taaattaggc
    18961 tcaatatcaa acagtatttg ctgattttgg cttaaaatct cggtattttt ttgtatggcg
    19021 gagattaacg ccgacatctg gacctcattg tgttcggaat cattggaatg ctcgatttta
    19081 accaaaagtg agagttggcg aagcaggtta gtcatacgtt gactttgctc tttcatcgca
    19141 ttgagcgctt tttgctgtaa tgccatatta tgcggctcgt tttccagcat ttccaaataa
    19201 ccttgtaata tggtaagagg tgttcgcagt tcgtggttca tatttgccaa aaaggtttgt
    19261 cgtgagtgca taaggcggac aaaagaggtt acatctcgtg cgacaataag tctggagtta
    19321 ctgtcatatc gattgagatt caattcaata tagcgtttat tatgagtcag cagtaccaat
    19381 gggcgatggt gtcgttgttg gcggaaatag cttttaaatt caggataaaa aatcacatta
    19441 aaaatgcttt tatttgattt tttgtcccaa taaaactcaa atagctcttg tgcaacatta
    19501 ttgcaccaag aaatctcccc gttttcttgg caaataatga tcgcatcggg taagtattga
    19561 atatgcttat ttaattttga taacagacgt aaggttttga ttttatcttg gcgtaaacgt
    19621 tcgtgataga atgcggcact ttgagataaa tgctccattg tcgttacttt tgacctgccg
    19681 ctttgctcat taggtctgag taactgaagg agagaagatt cggtgtggtg atgccaaata
    19741 agtagaacca gtaaacaaaa actaaaccag aaaaaataag cagggaaaaa cagattgaat
    19801 acgccggcaa cggctgctgc cagcaaaagt tccaataaaa agtgtttaag tgagagctta
    19861 actttcatta ttagtcctga aaatggttgg aaaaacggta tcccgagcca cgtacggttt
    19921 gaatataacg ctcaaaacca tagggttcaa gactcttacg caatcgccga atataagagt
    19981 ctaccgtgcg atcctcaaca taaatatcat taccccaaat aaaatcaagc aactgttcac
    20041 gacgatacac tttttcaggg tgagtcatta aaaagtgaag cagtttatat tccgtgccgc
    20101 ttaatttcac ttcctgttcg gcaaccaaaa cccgttgtgc ctgttgatca agaatcaatc
    20161 cgtctatatt gatgagattg cttggcggat catagcttcg ccgccatact gcttcaatcc
    20221 gggcaagcaa cacctgtggt gaaaacggtt tggtaatgta gtcgtctgca ccggcattca
    20281 gaccggtaat acagtcttct tcgctactgc gggctgttag cataatgatt ggaatttttt
    20341 cggtttcagg ctgttttttt aggtatttaa ttaaatggat acccgaacgc cccggcatca
    20401 tccaatccag taaaataagc tcgggttttt cgcccagttt attgatagca ctttggtagt
    20461 cttccgcttc aattacctga tattgtttgc tgagatagag acttagcatt tcacgaatag
    20521 cacgttcgtc ttcaacgatc agaattttgt tattcattct tcctctccga tgcggtgtga
    20581 caagcggtcg atttatcgca gaaatttgca aattttttac taaaaccgac cgcttgcaga
    20641 ttaccccatt ttacctttaa tgtaatcttc cgtctgccgg aattttggtt tgtcgaaaat
    20701 ttgtttggta tcaccgaatt caatcagctc gcctagatac atataagcgg tatagtcaga
    20761 acagcgggct gcttgctgca tattgtgggt aactatcgct accgtgtagt cgtgcttgag
    20821 ttcgttgata agttcttcga ttttcatagt cgaaatcggg tcaagtgcgg agcagggctc
    20881 atccagcagt aacacctcgg gtttcactgc aatacctcgt gcaatacaga gccgttgctg
    20941 ttgcccgccg gagagactgt caccattttg gtttaattta tgctttacct cattccataa
    21001 agccgctttg gttaatgccc actctacacg atcgtttagc tcggatttcg gcaatttttc
    21061 aaacagacga ataccaaaag caacattatc gtaaatggac atcgggaatg gggtcggttt
    21121 ttggaaaacc ataccgactt tggctcgaat aatcgaaata tccgtatcgg aggtgagtag
    21181 attctctcca tcaagattga tttcccccgt tgcacgttga ttcgggtaga gctcaaacat
    21241 tcggtttagg gttcgcagta atgttgattt tccacaaccg gaaggaccga taaatgcggt
    21301 cactttattt ttggcaatac gcatattgat atttttcaga gcgtggaaat cgccataata
    21361 aaaattgagg ttgttaatat gtaattttgt ctctaaatcc gttggtaaca tatagagtcc
    21421 ttattttgtt ttgctgaaat acagacgagt gatgatatta agagaaagca caaacagagt
    21481 aataagcatt gcacctgccc aagccaatgt attccagtca gcgaacgggc tggctgcgta
    21541 ttgataaatc accacaggga ggtttgccat tggtgcattc atatctaaag aggcaaactg
    21601 gttggataat gcggtaaaca atagcggtgc ggtttctccg gaaatacggg caacagcgag
    21661 caacacgccg gtaataatgc cggagcgggc ggcacgataa gagatcattg taattaccct
    21721 ccatcgtggg cagccgagag ccgcagctgc ttcacgcaga ttattaggca caagattgag
    21781 catactgtcg gtagttcgaa caacaatcgg aatcactaat accgccaatg ctaatgagcc
    21841 tgcccagcct gaatagtgtt taacctgtgc gacataaatc gcataaataa ataagccgat
    21901 aataatagaa ggagcagaga gcaacacatc attcaaaaaa cgggtgaatt ttgccaattt
    21961 ggtataacgc ccgtattcgc tcaagtaagt gccggctaaa ataccgattg gtgttgcaat
    22021 cagtgttgcg gtactgacaa tcagtataga gccgacaatt gcattacgta atcctcctgt
    22081 ttcatttggt gcaggagtcg ggcgggtaaa cagttcccaa gacagtgcag ggataccttt
    22141 ttggagtaaa gtaacaataa tccagaccag ccaaaataag ccaaataata cagcggaata
    22201 ggcaagactt aacatcaaga cattttttat ttttcggcaa taaaagcgtc ggttttggta
    22261 atttttcatt ctcgttttcc ttcctgttta cccattcgtg cgattaataa acgtgaagcg
    22321 gctaaaacaa tcgaagtaat aaggaacaga attagcccta ggctcatcaa cgctgatttt
    22381 tgtaatccgc cggcttcatt aaattcattg gcgatagcag aagcgatgga ggttgaaggg
    22441 gaaaataccg aatcaggtag cataaaggca ttaccgatca caaaggtaac tgccattgtt
    22501 tcgcctaatg ctcgacctaa cccgagcata attgcaccga ttactccggt tttggtgtaa
    22561 ggtagcacga ctttccaaat aacttcccaa gtggttgagc ctaaaccgta agcagactct
    22621 ttcagtaaag gcggtacaac actgaaaaca tcacgcataa ttgaggcaat aaagggaata
    22681 atcataatcg ccaataccaa gccggcactg aatagcccga tcccgaaagg agctccgcta
    22741 aaaagcatac cgataaaggg aagctgacca aatgtatcta tcaaatacgg ttgcacatat
    22801 tcttgaaata agggtacaaa tacaaacagc ccccacatac cgtaaataat agagggaata
    22861 gccgctagca tttctaccgc tgtgccgaca gggcgtttta gccattgcgg tgcaagttcg
    22921 gttaagaaag tcgcaatgcc gaacgagacc ggaacggcaa tgactaatgc aatgccggca
    22981 gaaatcaatg tgccgataat cggcactaat gcaccgtatc ggtcattcac agggttccac
    23041 tcgttttccc ataggaatga taccccgaat cggcttaatg cttcccaact gccaagcaat
    23101 aatgaaacca gcaccaccac caacaaggca aaaaccagca aagcaaagaa acgggttgtg
    23161 tgatgaaata acccctctac ccaaggatgg tttaagcatt tgttatcaat tttatgttta
    23221 tgcatagtga caactctttt gcaaaatgtt gtgaaaaaac gaccgcttgt tgttagttaa
    23281 gctccataca agcggtcgtt ttgcattagt agagcgaatt tccttgtcca tctttaactt
    23341 ccgttttcca ttgagtttgg attttctcta ctacattatc cggtagaggc acataatcca
    23401 gttcggcagc cgcatcttta ccgtgcttaa atgcccagtc gaagaattta aatacccctt
    23461 tagtttgctc tggtttgtcg ctatgttgat gaatcaagat aaaactggcg gcggtaatcg
    23521 gccaggcttt ttctcccgct tcgttggtta aaataacgcc cataccggct gtttggctcc
    23581 aatcggcgtt agcagcggca gcagcaaacg tttcacggga aggctgaaca aatttaccat
    23641 ctgcattttg catagaaacc caagccagag cattttgttt agcataggca tattctacgt
    23701 aaccaataga atacttgagc tgtttcacat aagctgcgac accttcattt ccttttccgc
    23761 cttgtccgac cggccatttc accgattttc cttcgccgac tttctccttc cattcggcag
    23821 agactttaga aagatagttt gtccaaccga aagttgtgcc tgaaccgtct gagcggcgta
    23881 cgactaaaat tgtttttgcc ggcagggcga tatcttgatt aagtgcgaca attgccggat
    23941 cgttccattg tgtaattttg cctaggaata tatcggcaag aactttaccg gacagtttca
    24001 gttcgcccga tttaagttca ggaatattga caaccggtac agttccccca atgatggcgg
    24061 gaaattgcaa aagtttgtgt tctgctaatg tttcgccttt catcgggtca tcagaagcac
    24121 cgaaatcaat tgttttggca ataatttgtt gctgaccgcc accggaaccg atagattggt
    24181 aattgatttt attaccggtc tctttttcat ataaacctgc ccatttggcg tagataggat
    24241 aagggaaaga agcaccggct cctgtgatgg tttgagcatt tgctgcgaca gaaagtgaaa
    24301 aagaaatgcc taaaacggta agtagtttaa atcgtttggc aatcatattg ttctccttaa
    24361 agttagcgtg agttaatttc acgtgcttag tgtagaaaaa gaatatgaca aaatgatgac
    24421 aatggtaaaa aaatcagaaa aaacgaccgc ttgtgtttca ggctagacta aacaggctaa
    24481 atcgtagaaa atagccctcg tttttttaat ataaatatcg ataaaaaatg accacagaaa
    24541 tcaattatga aggcattgaa cagatgccga ttaagcgttt taccgaagat gcttacctca
    24601 attattcgat gtatgtaatt atggaccggg cattgccgtt tattggcgat ggcttgaaac
    24661 cggtgcaacg ccgtattatt tatgcgatgt cggaacttgg gctaaacgcc tcggcaaagt
    24721 ataaaaaatc agcccgtacc gtgggggatg tattaggtaa attccatccg cacggtgata
    24781 gtgcctgcta tgaagcaatg gtgctaatgg ctcaaccgtt ctcttaccgc tatccgttag
    24841 tggacggaca aggcaactgg ggggctccgg acgatccgaa atccttcgct gcaatgcgtt
    24901 ataccgaatc gaaactgtct aaaattgcag aaattttatt gggcgagtta gggcaaggaa
    24961 cggtggatta tcagccaaat tttgatggct cgttggaaga accgaaatat ctgccggctc
    25021 gtttaccgca tattttgttg aatggcacga tggggattgc ggtgggaatg gcaaccgata
    25081 ttccaccaca caatattaat gaattggctg atgccagtgt gatgttgctg gataacccga
    25141 aagcaacgct tgatgatgta ctaagcgtgg tacaagggcc ggattacccg acagaggctg
    25201 aaatcatcac accaaaagcg gaaattgcca gaatgtatga gcaggggcgt ggttctatta
    25261 aaatgcgtgc ggtgtggaaa aaagaagagg gtgaaattgt gatttcagcc ttgccgcacc
    25321 aagcctctcc ttcaaaaatt attgagcaag tagcaaccca aatgcggaat aaaaaactac
    25381 cgatggtgga cgatattcgt gatgaatctg accacgagaa cccgattcgc attgtgattg
    25441 tgccacgctc taatcgcatt gattttgatg cgttaatgga tcacctgttt gcgacgaccg
    25501 atttggagaa aagctaccga gtcaatatga atatgattgg cttagacggc aagccggcag
    25561 tgaagaattt gctgactatt ctcaatgagt ggctgagttt tagacgtacc actgtcactc
    25621 gccgcctgaa ttaccgatta gataaaattt taaaccgctt gcatatttta gacggtttga
    25681 tgattgcatt tctcaatatt gatgaagtga ttgagattat tcgcaatgaa gatgagccga
    25741 aagccgagtt aatggctcgt tttaatctga ccgatgtgca agcagaggcg atcctgaact
    25801 tacgtttacg ccacttagcc aaattggaag agcatcaatt acaggcggaa aaatccgaac
    25861 tggaaaaaga acgtgatgaa ttgcaactga ttttaggctc ggaacgccgt ttgaacagcc
    25921 tgatcaagaa agagattcaa gccgatgcca aagcatttgc cagcccacgc cgctcaccat
    25981 tggttgaacg agcggagtct aaagcgattg cggaaagtga cttgactcca accgaagatg
    26041 tgactgtgat tttgtctgaa aaaggctggg tacgttgtgc gaaaggacac gatattgatg
    26101 ttcaagccct aagccatcga gccggagatg gttatcttgc ccacgcaagg ggtagaagta
    26161 atcagcctgt ggtattttta gacagtacag ggcgagctta tgctctcgat ccaacttcac
    26221 tgccttcggc acgctcgcaa ggcgaaccgc ttacaggcaa aatcacccta ccggaaggtg
    26281 cagtagtgca gcaagtgcta atggcaagtc cagaaactaa agtgctaatg gcatccgatt
    26341 caggttacgg ctttatttgc acctttgcag atttagtttc tcgtaataaa gccggcaaag
    26401 cggttatttc tttaaccgaa aatgcaaaag tactgccgcc gcaattattg gaaaatgatg
    26461 aaaatttatc gcttgtagcg atgagtaatg ttggcagaat gttggttttc ccggtgagtg
    26521 aattacctca actttccaaa ggtaaaggca ataaaatcat caatatttcc gccgcagcgg
    26581 caaaatccgg tgatgaatat ttagcccgtt tattagtcat caaaccaacc aactcgctgg
    26641 tctttgtgtc cggtaaacgc aaaatcacat taaaaccgag cgatgttgat aactaccgtg
    26701 gcgaacgagc aagaaaaggc tcacagctag tgagagggct tagtaccaat tcaacggtag
    26761 agattgttga gtaaagagaa gaagcggtta gggggtaaat ccaacaattt tgccccaaat
    26821 aaccgcttgc aatggagtta ttgcttattc aaataagcct ttgtgaagcg aacgaaccac
    26881 atcgtctgct tctgcatttt tcactaaggt acaaacatta ttgctgcttg ctccatagct
    26941 gattaggcga atgttgtaat tctcaagagt gtggaataat tttttcgcca caccggactg
    27001 agcgtgtaag ttgttgccga taatagcgac taaagcgagg tcgctttcta cttgcacatt
    27061 gcaatatgcg ttaagctcat cgagtaattc cgctgataac atatctgcac ctgaagaggc
    27121 tgagcccgtt ttatctaggg tcaatgcaac gctgacttcg gaagtggtaa tcacatcaac
    27181 cgaaattttg tgtttcgcta aaatcgcaaa tacattcgct aagaaacctt gtgcgtgtag
    27241 cattgaaagg ctggaaagcg tgagtaaaat ttggttgcgg cgtaatgcaa tcgcacggaa
    27301 agtcgggcgt ggttgtggat cacgggttac ccaagtgccg ccttgttccg gtgctttgct
    27361 tgagccaaca tatacaggga tattgctacg cactgccggt aataaggttg ctgggtgtaa
    27421 tactttcgct ccaaatgttg ccatttctgc cgcttcgtta aatgccattg tgtcaatgcg
    27481 ttgagcgttc ggtacaacac gaggatcggt ggtataaatc cccggtacgt ctgtccaaat
    27541 gagtacatcg ttagcattta atacttctgc taacaatgca gcagagtagt ctgaaccgcc
    27601 acgtcctaaa gtagtggttt tgccttcatc atcacgacca ataaagccct gtgtaatcac
    27661 aattttgcct gatgcgatta atggcttaat cacattgtct gattgttgct gggttttctc
    27721 gtcatttggt gctgcttttc cataatggct gtttgttgcc acaacgtcac gcacatctac
    27781 ccatactgcc gggtgtccac gttcaattaa taattgcgtg aagattttgg tggacatcat
    27841 ttccccatgg ctgattaact catcacttaa ggcagctgaa gtagcaaggc tggctgattc
    27901 tgctaatgtt tcaatacgtt ctaataattc gtcgatttcc gctttaacgg catcttgatg
    27961 ttgtaatttt tcaataatat tgtattgaat ttggcggact gctgcgatga cttcatcacg
    28021 gtgttctttt tcacagccgt tagcaagttc cactaagtaa ttggttacgc cagcagaggc
    28081 agaaagtacc accacacgag tatttgcatc agcaattaca atattggcag aagaggtcat
    28141 tgcatcaaaa tttgcaacac ttgtaccacc aaatttagcc acagaaagat gagacataat
    28201 aaaaatttcc ttaaaacaaa aatagataaa tattgtgtta ggggatattc ggaaaggaaa
    28261 tttgagtaga ttatacggtt ttattgttga accaattaat cagagcactc catttaatat
    28321 ggtgaaataa cctattaaat gacagctgat aggattcaac ctattcagcc gatgttaatt
    28381 ccagtctagt gaattcacac ctcggcaatc actcccatca cttaattctc aaacggccct
    28441 agttgcctaa aatcaagttg cctgagtgtt gcacctctcc cgtaatggca agcccattac
    28501 tctttttcaa cagtattgaa aagtgtgcat agaaataccg ttttattaat gttttgtcaa
    28561 tgaatattta
//
LOCUS       DANLUQ010000012        27993 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.012, whole genome shotgun sequence.
ACCESSION   DANLUQ010000012 DANLUQ010000000
VERSION     DANLUQ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 27993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27993
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.012"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            197..2764
                     /gene="clpB"
                     /locus_tag="PXB27_000444"
     CDS             197..2764
                     /gene="clpB"
                     /locus_tag="PXB27_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDL5345271.1"
                     /translation="MNFEKMTTKLQEALAESQSLAVGKDNPYIEPAHLLYALLKQEGG
                     SIASLFTTLNVDVPTLIRELQQILDRLPKVQGGNTQVSQQLVRLLNQSDKLAQQFGDS
                     FISSELFVLAALDDNGDLGKLFKQFGLNKEKLTQAISQIRGGDTVNNQNAEDTRQALK
                     KYTIDLTERAKAGKLDPVIGRDEEIRRAVQVLQRRTKNNPVLIGEPGVGKTAIVEGLA
                     QRIVNGEVPEGLKNKRVLSLDMGALIAGAKYRGEFEERLKAVLNDLAKEEGQVILFID
                     EIHTMVGAGKTDGAMDAGNLLKPSLARGELHCVGATTLDEYRQYIEKDAALERRFQKV
                     LVDEPTVEDTIAILRGLKERYEIHHHVQITDPAIVAAATLSHRYVSDRQLPDKAIDLI
                     DEAASSLRMEIDSKPEPLDKLERRIIQLKLERQALQKEEDEASRQRLAKLDEEMAAKE
                     REYSELEEIWKAEKSALLGTQHIKSELENARIAMDQARRENDFEKMSELQYGVIPNLE
                     KQLQAVENGEKNADAQLLRTKVTEEEIAEVLSKATGIPVAKMMEGEKEKLLRMEEVLH
                     ARVIGQNEAVDAVANAIRRSRAGLSDPNKPIGSFLFLGPTGVGKTELSKTLANFLFDD
                     PDAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRHPYSVVLLDEVEKAH
                     PDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSHLIRENAYKGYDVVKEMVM
                     EVVGQHFRPEFINRIDETVMFHSLGQEHIRSIAKIQLQRLIQRLAERGYEVTVTDAAL
                     DHIGKAGFDPLFGARPLKRAIQQELENPLAQQILSGKLLPNKAVTVDYQNGQIVATQ"
     gene            2978..4120
                     /locus_tag="PXB27_000445"
     CDS             2978..4120
                     /locus_tag="PXB27_000445"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDL5345272.1"
                     /translation="MSQLFPEATIRTQAPYRNDIVGSFLRTDALKKAREDFNQQKITR
                     DELREVTRQEVKKLVELQKQHGVKAVSDGEFSRTWWHLDFLAELDGMDWQETEVFNVN
                     FTGHKPKGQTVKIVGDIDFSESHPFVDAYRLLKEAAGDYPTKFTIPAPTMLHIICCVR
                     AEDYQPLPQYQDEQKLYDDIANAYIKAMHKFYDMGLRNLQLDDTSWGQFCAEDKRKEF
                     AARGFNLTDLAEKYVALLNRIVDAKPADMSITMHICRGNFRSSWFSEGGYGPVAEVLF
                     GKCRVDGFFLEYDTERAGTFEPLRHIKDQQVVLGLITSKFGDLEDKNVIIERIKEAAQ
                     IVPINQLCLSPQCGFASTEEGNILTEAAQWAKLNLIKEIAEEVWGS"
     gene            complement(4177..5568)
                     /locus_tag="PXB27_000446"
     CDS             complement(4177..5568)
                     /locus_tag="PXB27_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HDL5345273.1"
                     /translation="MENTPLIEPSKNTRNSLIFVADAILFIILLNTLPFTPEANKGLA
                     LLIFIAVLWLTEALHVTVTALLIPILAVALGLVKSKEALVAFADPTIFLFFGGFALAT
                     ALHIQQLDRMIANKIMALAKGKLSIAVIYLFAVTAFLSMWMSNTATAAMMLPLAMGIL
                     SKLDQKQNHNTYLFVLLGIAYSASIGGMGTLVGSPPNAIVASQLNLTFADWLKYGLPI
                     MLILFPLMIATLYIVFKPNFSVSFDRSFEKIELNRSRIITLAIFVFIALGWIFGDKIN
                     PIISAFLGINGKIASFDTILALIAAALICITRVANWQQIQENTEWGVLFLFGGGLTLS
                     AVLGQTGASKIMADGVVSLIEGGHFYLIGLIVAAFIIFLTEVTSNTASAALLVPIFIS
                     IAQALNMPPVGLALIIGLGASCAFMLPVATPPNAIVFGTGQIKQSEMIKVGFWLNIIS
                     IFVIATVGYLFWL"
     gene            complement(5728..6627)
                     /gene="cdd"
                     /locus_tag="PXB27_000447"
     CDS             complement(5728..6627)
                     /gene="cdd"
                     /locus_tag="PXB27_000447"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDL5345274.1"
                     /translation="MFRTDITERFEQLKAAKNCEVTQAIIEQLIKQDFHGQLSYEVVD
                     ELCEKFKRSRVELALYCIAIAACYAVTPVSDFNVGAVAIGKNGDFYFGANQEFSGECM
                     QQSVHAEQSAISHAFLAGETLITDVVVNYTPCGHCRQFMNELNSAASLKVHLPHSQNN
                     LLHSYLPDSFGPKDLGIEKVLFDEQPQQFEVKGDHLQQAAIFAANNAYAPYSNAFSGV
                     ALQVGERIVCGKYTENAAFNPTFLPLQSALNYRLFLGLADIPVTRVVLAEAKGSLSSY
                     AMTQALAKSLFGLEIEYLSLQAV"
     gene            complement(6641..7543)
                     /locus_tag="PXB27_000448"
     CDS             complement(6641..7543)
                     /locus_tag="PXB27_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL5345275.1"
                     /translation="MISSSLKLGYFSLIVATLFWGGNFVLGKVLSGAVQPITLTYLRW
                     LPAFLILVLLFFRPTWRVMHLLKPRLGVMWVLGLLGVILFPATLYEGLKTTTALNASL
                     YLAISPVLVLFLNRLVFKEKISPIILSGAIISLLGVLWLLMQGKLTRLFSLAINQGDL
                     WAIASTISWAVYCCVIRLRPQGISNTVFLTVLVGLAVLTLTPFFIYEYVAESAKNLAN
                     LTALQWSGIGYLVIGPSILSYAFWNFGIAIVGSAKGAVMTNFTPLFAAMFSIILLNES
                     VHTYHIISAILITAGILICSYKKH"
     gene            complement(7540..7896)
                     /gene="folB"
                     /locus_tag="PXB27_000449"
     CDS             complement(7540..7896)
                     /gene="folB"
                     /locus_tag="PXB27_000449"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HDL5345276.1"
                     /translation="MSDKVFIRELTAFASIGAYNWEHTIKQRLVFNIDMAWDFSKAAE
                     TDDVQYCLNYAEVSQKVLDFVESQPFKLVETVAYQLAELLQKEYQLSWIRIELHKPKA
                     VAQASSVGVIVERGHQ"
     gene            7966..8571
                     /gene="plsY"
                     /locus_tag="PXB27_000450"
     CDS             7966..8571
                     /gene="plsY"
                     /locus_tag="PXB27_000450"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDL5345277.1"
                     /translation="MNLIPYLLILTAYLLGSISSAVIFCKLAGLPDPREVGSHNAGAT
                     NVLRIGGKSAALGVLLFDILKGTLPVAMGIYFELSPSQLGFIALAVCLGHIFPIFFQF
                     RGGKGVATAFGAISALSFIVAGGAIGTWLLVFLISGYSSLSAVFTALVVPFYIWWFEP
                     QFTFPVALVCCLLVYRHHDNIQRLWRGQEDKLWDKFKKNRA"
     gene            complement(8645..10552)
                     /gene="parE"
                     /locus_tag="PXB27_000451"
     CDS             complement(8645..10552)
                     /gene="parE"
                     /locus_tag="PXB27_000451"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDL5345278.1"
                     /translation="MSEKRYGADEITVLKDLEPVQLRPGMYTDTTRPNHLGQEVIDNS
                     VDEALSGFADKIDVILHADNSLEVVDNGRGMPVDIHSTEKISGVELILTKLHAGGKFS
                     NKNYTFSGGLHGVGISVVNALSERVEIQIKRGGEVYSIAFANGVKVEDLTVIGTCPKK
                     QTGTTVRFYPNPKYFDSPRFSVSRLRHLLRAKAVLCPKLTINFIDKINNTEESWYYED
                     GLSDYLSEALNGYETVPNPPFIGDVTAETEAVSWALTWLPEGGELVAESYVNLIPTAQ
                     GGTHVNGLRNGLLKAMVEFCEIHNLLPKGVKLTADDVWNRCAYVLSLKIQEPQFAGQT
                     KERLSSRQASSYVDSTLKDAFSLWLNQNVQTGKLIAEMAISSAQSRMRAAKKVVRKKL
                     VSGPALPGKLADCTAQDLSRTELFLVEGDSAGGSAKQARDKEYQAILPLRGKILNTWE
                     VSSDQVLASQEVHDIAVALGIDPDSDNLEELRYGKVCILADADSDGLHIATLLCALFL
                     RHFPKLVQNGHVYVAMPPLYRIDIGKDEVHYALDEAEKEAILARLAKKKGKPNVQRFK
                     GLGEMNPSQLRETTMEPSTRRLVQLTFDDTVEEGELNTFEIMDMLLGKKRAEDRKQWL
                     QNRGDEADLAV"
     gene            10760..10984
                     /locus_tag="PXB27_000452"
     CDS             10760..10984
                     /locus_tag="PXB27_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDL5345279.1"
                     /translation="MVVKILKRKSQKTLQQIYTQPISANIKWTNIEALFLELGAEIEE
                     REGSRIAVVLFQQVRVFHRPHPNPDTDKGR"
     gene            11027..11356
                     /locus_tag="PXB27_000453"
     CDS             11027..11356
                     /locus_tag="PXB27_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDL5345280.1"
                     /translation="MRNIMDINGYKAVIAYDPETELFRGEFIGLNGGADFYADNVIQL
                     KKEGELSLNIFLELCREKGIEPFKQYSGKFNVRLSPELHKAAVIAATAENLSLNEWIN
                     QTLEKSV"
     gene            complement(11395..11781)
                     /gene="cueR"
                     /locus_tag="PXB27_000454"
     CDS             complement(11395..11781)
                     /gene="cueR"
                     /locus_tag="PXB27_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HDL5345281.1"
                     /translation="MNISQAAEKTGLSAKQIRDYEKAGLLPPTSRTEAGYRFYNATDL
                     SRLHFISNARKVGFSLAQISELLKLNDNPNRSSRDVKQLTEQHIGELEQKIADLNQML
                     TLLKSWSSSCCGNANSECSILNGLNG"
     gene            11870..12070
                     /locus_tag="PXB27_000455"
     CDS             11870..12070
                     /locus_tag="PXB27_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HDL5345282.1"
                     /translation="MTITLQLDGLHCGNCVKSVEKALNGVAGVNQVSVTLEPQIAVVE
                     GSAEAQALIEAVEDIGFDAKLA"
     gene            12123..14315
                     /locus_tag="PXB27_000456"
     CDS             12123..14315
                     /locus_tag="PXB27_000456"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005710411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDL5345283.1"
                     /translation="MTSTAHYSPEQQLLIDGMHCAACVRRVEKALLKVEKVEFASVNL
                     ADQTAFVQGNANPEALVAAVVKIGFGAEVLESEEERRIKQQAQTQRVLGHKRTQFMLA
                     LVVGFALVAFGFYKGMTVNETNRSLWLSWAGISLATMYFSGKDFFLGAFSALKNRSAN
                     MDMLVAISTGTAWLYSCWLTLFPQPNAHVYFEASVMILGFINLGKYLELKAKQRSSLA
                     LEKLLDLAPKQAVIFEENIAKTIPAKAIKPQMRVQALTGDRLAVDGILDSGSIWVDES
                     MLTGEALPVEKKIGDKVRAGTLVQDGSGTYIAEQVGSQTALARVINAVRHAQSSKPPL
                     AQFVDKIAAVFVPVVVSIALLSALIWLAVGQDFAFALSIFTTVLIIACPCALGLAIPL
                     STIAGVARSAEFGVLVRNIEALQASSEIDTLVFDKTGTLTTGRMEVSDVLTFNGFEQK
                     RLLQLAKSVEQHASHPIAKAIAKFAENQTACGVTDVQVLKGLGISAKYEGSTIRMGNW
                     QFVGETIQANLASLLPAKEGDFSSKIFVSVDNQLAGVLSVSDQLRPEAKATIHQFQAE
                     GYQCVMLTGDRQQTADYFANALGLDGVIAEVLPEQKAEKIRALQAEGKKVAMIGDGIN
                     DAPALAQANVGIAMHNGSDIAVETADLSLMREGLAPLMQILPFSKKVLKNMKQSLLGA
                     FIYNVIAIPIAAGVLYPFTGWLLNPMIAAIAMTLSSITVVLNSQRLLK"
     gene            complement(14368..15153)
                     /gene="truA"
                     /locus_tag="PXB27_000457"
     CDS             complement(14368..15153)
                     /gene="truA"
                     /locus_tag="PXB27_000457"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDL5345284.1"
                     /translation="MKIALGIEYDGSRYFGWQRQEAVESVQQQLEQALSVVANSPVEV
                     FCAGRTDAGVHGTGQVVHFETQANRPLQSWCFGTNAHLPDDIAVKWAVAVNEDFHARF
                     SATARRYRYIIFNQKLRSAILPKGVTHFHFPLDERKMHQAGQYLLGENDFSSFRAAKC
                     QSNTPWRNIHHLNVSRQGNYVIVDIQANAFVHHMVRNIVGTLLEIGQGRQPVEWAKWV
                     LEQRDREKAAPTAKAEGLYLVEVHYPEHFGIPKTPLGPLFLAD"
     gene            complement(15218..15907)
                     /gene="folE"
                     /locus_tag="PXB27_000458"
     CDS             complement(15218..15907)
                     /gene="folE"
                     /locus_tag="PXB27_000458"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HDL5345285.1"
                     /translation="MTTISPELTITENTLSPEAQKVRQALLAKGIETPTVTHTKDKAS
                     RRAEIQQHMQAVLELLGLDLSDDSLEETPHRLAKMYVDEIFSGLDYATFPKITNIENR
                     MKVSEMVLVDDITLTSTCEHHFVTIDGKVAVAYYPKKWVIGLSKINRVVQFFAQRPQV
                     QERFTEQILTAFQTILETDDVAVYVKATHFCVKCRGIKDTNSYTVTSAFGGVFLEDRE
                     TRKEFLGLLNK"
     gene            16008..16715
                     /locus_tag="PXB27_000459"
     CDS             16008..16715
                     /locus_tag="PXB27_000459"
                     /EC_number="2.7.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid kinase"
                     /protein_id="HDL5345286.1"
                     /translation="MQNIQYLFNADLIPEHQAEFVQSLLTCKNFDNSDRLLGFSKGRG
                     TTWFLDTSAELGINVVLRHYYRGGLFGKIIKDSYFFTRFEATRAYKEFFLLQQMLAWG
                     LPVPRPVAIKIERHFCCYRADIMLEKLENTQDLSKILQSHSLSNEQYEQIGKLIRSLH
                     NHQIHHSDLNIHNILLGNDGKFCLIDFDKCGIEPGENWKQNNLDRLLRSFKKEQGRLN
                     IFFSEENWQAVLRGYTL"
     gene            complement(16770..17867)
                     /gene="apbC"
                     /locus_tag="PXB27_000460"
     CDS             complement(16770..17867)
                     /gene="apbC"
                     /locus_tag="PXB27_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HDL5345287.1"
                     /translation="MNQLNEQQLVEIKAVLQQFSHPTLQKDLIALNAFKKAELGGDIL
                     RIEITMPFAWNSGFETLKAETEAKLKQVTGSSGVKWVLNYQIATLKRANKHPAVNGVK
                     NIIAVTSGKGGVGKSTTSVNLALALRAQGTRVGILDADIYGPSIPHMLGAKDQRPTSP
                     DNKHITPITAHGLQSNSIGYLMEEDNATIWRGPMASSALSQLLNETWWNELDYLVIDM
                     PPGTGDIQLTLSQQIPVTGAVVVTTPQDIALLDAIKGISMFEKVSVPVLGVIENMSMH
                     ICSNCGHHEAIFGTGGAEKIAQKYGTKVLAQLPLHIRLREDLDSGVPTVVAAPEHEIS
                     QAYLALAEKVASELYWQGSVIPSEIMIREVK"
     gene            complement(17864..19411)
                     /locus_tag="PXB27_000461"
     CDS             complement(17864..19411)
                     /locus_tag="PXB27_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL5345288.1"
                     /translation="MLKSLQAVTFVKKIAKVTACILLITSCDQLICPQSIQSVWVENR
                     QSDTLLKRAIYSAVFQLDPHKIKASADAAPLRDLLVGLTAYNRKGDVVPAIAQNWFTE
                     DEKEWLFILDEKAVWSNGQPVTAQDFVASWRRLIDPQNHSVLSKYLIYMGIENAKAIE
                     EQIKSVEELGVEALNAHTLRIRLNHPNHQLPEMLAHSALLPTYQGIAPTIDNFVSNAS
                     YRLEKLDAQKMVLKARTQEIPFQTIEYDLITTIQNCSAFDIVENPLLNKKTNIVKFPR
                     LCSYYYEFNFDDPLLAKREIREAIRTMVASANSGQEYGVPSQSVIPHSLVKDREKRWN
                     PVVVEQLLTKAGISPYNPLKLTLLYDENSINIANQVTRALSQSELFNITPKMVDWQTL
                     NKVREQKDYQIARAGWCADYPDPIVFLQKFHSMSPDNHSGYKSEIVDQKLNRLQTETL
                     SAEARESLIQEISEQLYSDVAVLPLFQYHHRVGIVSSLLGIDLNNDSEVIYSKDLKRN
                     LANKDNQ"
     gene            complement(19437..20087)
                     /locus_tag="PXB27_000462"
     CDS             complement(19437..20087)
                     /locus_tag="PXB27_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchE family NAAT transporter"
                     /protein_id="HDL5345289.1"
                     /translation="MDIIINFAIYLQFFIGLFAIVNPFGSLPIFFSMTAHQYEGERNR
                     TSLITAVSIGVILLVSLYFGNLILNAFSISLSSFRVAGGILIVSIAMTMISGKLGEHK
                     QNKEEKNEDITEYENIGVVPLAMPIMAGPGAIGSTIVWGTRYHTWEDYVGFSIAIIVF
                     ALICYILFRYSAPLVKKLGKTGSNVVTRIMGLILMALGIEIIVAGITTLFPVLLVK"
     gene            complement(20209..22080)
                     /locus_tag="PXB27_000463"
     CDS             complement(20209..22080)
                     /locus_tag="PXB27_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HDL5345290.1"
                     /translation="MIEKMHERTNGPVFKIIFALVSISFVIGGIGTGLISQDNSVAKV
                     NGEEISQQLFNNTLNREQNRLNAELGSRFWDLMDSPEYAAQFNQSVLNGLIDEELLRQ
                     YAKELKLGISADQIKSEIVNSQMFQQDGKFSNELYQQTLRHNNLSADGYAAIVQEGML
                     QSQLQEGIINSDFNVPAQQALLAKLLLQQREVRLAEFSVAKEMENQTASEQELRAYYE
                     ANKTKLLAPEKLAVEYVTLSPKDVESKVEITNEQIQTYYDRNKADYVTKGEAHLAHIQ
                     VASEAEAQAIEQALKNGEDFATLAKNKSSDTLSANNGGDLGWAKAGTFPKAFEDAVAN
                     LTAGQMSAAVKVDNAYHIIKVLERKPEQVIELAQVKDKIAQTIRQELVLTEYFTVARE
                     MANKAFENNSSLEEVAKAGNVELQKTDLFTRENVPTALQNEKVLKVLFEGELRQNKQN
                     SDALDVGNDANPQTLFVRVSDYDAERPQTFDEAKAAVENFVKAEKAEKALLAKVEENV
                     KALNEGKSVDVKFNAPQTLVYMQAEVENPVLAGTIFAMKKAQDKTTYQMARNQKGDVV
                     IVALDNITEGNAEQFKTLAPQFEQANRLILRNEFLQSLRAKASIEVNEDFMQQRSSH"
     gene            22262..22531
                     /gene="rpsO"
                     /locus_tag="PXB27_000464"
     CDS             22262..22531
                     /gene="rpsO"
                     /locus_tag="PXB27_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDL5345291.1"
                     /translation="MSLSVEAKAKIVAEFGRDAKDTGSSEVQIALLTAQINHLQAHFA
                     EHKKDHHGRRGLLRMVSRRRKLLDYLKRTDLAKYSETIARLGLRR"
     gene            22603..23472
                     /locus_tag="PXB27_000465"
     CDS             22603..23472
                     /locus_tag="PXB27_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HDL5345292.1"
                     /translation="MAFASLFTLLDDIAAVLDDVSIMTKVAAKKTAGVIGDDLALNAN
                     QVSGKNVVPERELPIVWAVAKGSLINKVILIPLALLLSVFLPQAIIPLLMIGGAYLCF
                     EGVEKLLHRLLHKNNQAHSDETETLSEKDKIKGAIRTDFILSAEIIIIALGVLQDSST
                     LTQILSLSAIGVGITLFVYGLVGLIVKLDDIGLWLMQKKSTFSQAVGKGLLFAMPWFM
                     KGLSVIGTIAMFLVGGGIFTHNLPMIHHFVEQFGISPHFSTLVDFAVGIVVGIIACLI
                     ILPLMKLFSKKAH"
     gene            complement(23562..24827)
                     /locus_tag="PXB27_000466"
     CDS             complement(23562..24827)
                     /locus_tag="PXB27_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HDL5345293.1"
                     /translation="MFRTLFLMLLLLVGLISGPYLAGNQGYVRIETATTVIEMSIVML
                     VVFFVLAMAVVYLLEWIVTKICRMSNGAYNWFSTRKHKKAQQETLEGLMRMSEGNYSK
                     AEKLFSKNAKHADEPVLNLIKAAEAAQQNGDDLAANKYLIKATEIAGPNNVAVELVRT
                     RILLQQGKLPAARTAIDSLLELAPHSDEVLRLAIQIYKESRAYVALDRLLVEIGQRNF
                     LTAEEYAELEYFVDDGLQDERLQEEGQEGLLSWWENQPNRRRKSVYSRVGVIKRLIDS
                     DDHQSAQEIALETLKKFEDEQLQGLFVQLTRLQIDSDSKLLKVLAKRADKANVKYTDD
                     YARALGYIYTRDGQYQKAKSQFELLLAHDECVADDRIMALHVAEQMGDTLMANRIREE
                     NLKQVNMATQSKVNEPLALPQTATESNSL"
     gene            complement(24846..26156)
                     /locus_tag="PXB27_000467"
     CDS             complement(24846..26156)
                     /locus_tag="PXB27_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDL5345294.1"
                     /translation="MSRSKKVTEHLEHSAEKVVEEAQQAVDSAGSFAKKEIEEDVVNE
                     AIEKDIQTEMQKAPKEQVVVQKSGGKGLALVALLVALAVGGAGHYMANKKFAEVESQI
                     QAVSAKANQPTTQTMVEMPNFENEKAQIAELVTEYQKALERIKALETAQNGYTQQISS
                     LQLQLQKLNNTSGSDNTFWLLSEADFLLNNAARKVVLDNDIETAKNLLLEADQVLSKA
                     PNTIHIREAIKTDLNTLTALHQIDQNALMQRVANLANKLDDLPILESEQASNSKQDGQ
                     VSDSLADWEKNLEKSAASFLDHFIRVSKRNAADEKAFVAPNQEIYLRENIRLRLQIAI
                     LAVPRQQSELYKKSLQTVGSWVRSYFETSNENVQHFLKELDELAEQTIYVDAPTSLQS
                     LKVIAQQLNKTPEKIEKVEIKAEKELEQAEPVKAEQDAMPLSAQ"
     gene            complement(26168..26932)
                     /locus_tag="PXB27_000468"
     CDS             complement(26168..26932)
                     /locus_tag="PXB27_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HDL5345295.1"
                     /translation="MNVLVTRLDSRGQELVDMLNEQQIFAIHQPLFTIEAGKELPLLP
                     SAISRLKSDDYVFVVSPNAVDYAAKTLVDTGFHFRSDLKYFAVGQRTAKYLTEKAEQA
                     VIYPIEFENSEGVLALPEMQNLTDKTILILRADSGRELLAETAVLRGATIQYLECYRR
                     EPVTDDIPEKISLCKRLGVDTIVITSSEILRSLYEQAKADCRAWLFECDLVVVSRRIA
                     KIAKQMGWQEDKIRVSDKADNVTLMNTLLFKVGKSN"
     gene            complement(26932..27867)
                     /gene="hemC"
                     /locus_tag="PXB27_000469"
     CDS             complement(26932..27867)
                     /gene="hemC"
                     /locus_tag="PXB27_000469"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDL5345296.1"
                     /translation="MKDILRIATRQSPLALWQANFVKSELEKHFPQLSVELVTMVTKG
                     DIILDTPLAKIGGKGLFVKELELALLENRADIAVHSMKDVPMSFPEGLGLAVICERED
                     PRDAFVSNNYHSLEDLPKGAVVGTSSLRRQCQLMAKYPHLEVKSLRGNVGTRLSKLDN
                     GEYDAIILASAGLIRLGLQERIRSYISVEQSLPAAGQGAVGIETRANDERILNYIAKL
                     NHSSTACCVMAERAMNTRLQGGCQVPIGGYATLEGDELTLNALVGSLDGSQIIRASAK
                     GHKQEAEQLGILVAEQLLAQGADKILAAVYNENVQ"
BASE COUNT         8260 a         6066 c         5377 g         8290 t
ORIGIN      
        1 aaatgaaatg aaatagaaat gaattgatat ttatcaaact tttaacacaa agatacccaa
       61 caagagttat tttatttatt tttgtgatct ttttcacatt tttacttgaa aattgtttac
      121 caagctccat ttagtgtttc aacaagagat gtaaataaaa atttacactt tctaaactca
      181 ttgtaaggag aaagaaatga attttgaaaa aatgaccaca aaactgcaag aagccttggc
      241 tgaatcacag tcgcttgcgg taggaaaaga taatccgtac atcgaaccgg cacatttgct
      301 ctatgcgttg ttaaaacaag aaggtggctc gattgcttct ttgtttacca cattaaatgt
      361 tgatgtaccg actttaattc gtgaattaca acaaatttta gaccgcttgc cgaaagttca
      421 aggcggtaac acccaagttt cacaacagtt ggtcagactg ttaaatcaat cagacaaact
      481 ggcacaacaa ttcggcgaca gctttatttc atcagaacta tttgttctag ctgcacttga
      541 tgacaatggc gatttgggga aactatttaa acaatttggc ttaaacaagg aaaaactaac
      601 acaagccatc agccaaattc gaggaggaga cacagtgaat aatcaaaatg cggaagatac
      661 ccgccaagca ctcaaaaaat atacgattga tttaaccgaa agagccaaag ccggtaaatt
      721 agacccggtt atcgggcgtg atgaagaaat tcgccgtgcg gtgcaagtct tacaacgccg
      781 taccaaaaac aaccctgtgt taattggtga accgggtgtg gggaaaacgg cgattgtaga
      841 aggcttggca cagcgtattg taaacggcga agtgccggaa ggcttgaaaa ataagcgtgt
      901 actttcgctg gatatgggag cattaattgc cggtgcgaaa tatcgtggtg aatttgaaga
      961 gcgtttaaaa gcggttttaa acgacctggc gaaagaagaa gggcaagtta tcctctttat
     1021 tgatgaaatt cacacgatgg ttggagcagg caaaacagac ggagcaatgg acgccggcaa
     1081 cttattaaaa ccaagccttg cccgtggtga attgcactgt gtgggggcta caacccttga
     1141 tgaatatcgc caatacattg aaaaagacgc agcccttgaa cgccgtttcc aaaaagtatt
     1201 ggtggacgaa ccaacggttg aagatacgat tgcgatttta cgtggtttga aagagcgtta
     1261 tgaaatccat catcacgttc aaattacaga ccctgccatc gtggcggcgg caacgctttc
     1321 gcaccgttat gtgtctgacc gtcaattgcc ggataaagcg attgacttga ttgatgaagc
     1381 cgcttcaagc ctgcgtatgg agattgattc taaacctgag ccgttggata aattagaacg
     1441 ccgcattatc caactcaaat tggaacgcca agcgttacaa aaagaggaag atgaagcgag
     1501 ccgtcagcgt ttagccaaat tagacgaaga aatggcagcg aaagagcgtg aatattccga
     1561 gctggaagaa atctggaaag cggaaaaatc cgccttactt ggtacacagc atattaaatc
     1621 ggagttggaa aatgcccgaa ttgcgatgga tcaggctcgc cgtgaaaacg atttcgagaa
     1681 gatgtcggaa ttgcaatatg gcgtgattcc aaacttggaa aaacagttac aagcggtcga
     1741 aaatggcgaa aaaaatgcag atgctcagct cctccgtact aaagtcaccg aagaggaaat
     1801 tgcggaagtg ctttcaaaag caacgggcat tcctgttgca aaaatgatgg aaggcgagaa
     1861 ggaaaaactc cttcgtatgg aagaagtatt acacgctcgg gttatcgggc aaaatgaagc
     1921 cgttgatgcg gtggcaaatg cgattcgccg tagccgtgcc ggactttccg atccgaataa
     1981 accgattggt tcattcctat tcttaggacc gaccggcgtg ggtaaaaccg agttaagcaa
     2041 aaccttagct aatttcttgt ttgacgatcc tgatgctatg gtgcgaattg atatgtctga
     2101 atttatggaa aaacacagcg tatcccgctt agtgggcgca cctccgggct atgtcggcta
     2161 cgaagaaggc ggctatttga ccgaagcggt tcgccgccat ccatattcgg tagtgttact
     2221 tgatgaagtg gaaaaagctc accctgatgt gtttaacatt ttattgcaag tgttagatga
     2281 cgggcgttta accgacgggc aaggcagaac agtcgatttc cgcaatacgg tagtgattat
     2341 gacctcaaac ttaggctcgc atttaattcg agaaaatgcg tataaaggct atgatgtggt
     2401 taaagaaatg gtgatggaag tcgtcggaca gcacttccgc cctgaattta tcaaccgtat
     2461 tgatgaaacg gtgatgttcc actcacttgg tcaagagcat atccgttcga ttgcgaagat
     2521 tcagctacaa cgcttaatcc aacgcttggc agaacgaggc tatgaggtga cagtaaccga
     2581 tgcagcatta gatcatatcg gcaaggctgg tttcgatccg ctgttcggag caagaccact
     2641 gaaacgtgcg attcaacaag agcttgaaaa tccgcttgct caacagatct taagcggtaa
     2701 attattaccg aataaagccg ttacggtgga ttaccaaaac gggcagattg ttgcaacaca
     2761 ataatgtctc cgatggtgcg agcgtcctcg ctcgtacctt acaagcggtg gatttatcgt
     2821 aaaaatttgc aaaaaattgc tcgaaaataa ccgcttgcac agtaaaaatt ttcatattgc
     2881 ccttcattaa ttttcataag ctaatgaagg gctttttatt actctttttg ctatcattaa
     2941 cggcgtttat aaccaataca attacaagga gtaatttatg agccaattat ttccggaagc
     3001 aacaatccgc actcaagccc cgtatcgtaa tgatattgta ggcagctttc tacgtaccga
     3061 tgcgttaaaa aaagccagag aagatttcaa tcagcaaaaa atcacccgtg acgaattgcg
     3121 tgaagtcaca cgccaagagg tcaaaaaatt agttgagttg caaaagcaac acggtgtaaa
     3181 agcagtctct gacggtgaat ttagccggac ttggtggcat ttagatttct tagctgaact
     3241 tgatggaatg gattggcaag aaaccgaagt ctttaacgtg aactttaccg gtcataaacc
     3301 caaagggcaa acggtaaaaa tcgtgggcga tattgatttc tccgaaagcc acccttttgt
     3361 ggacgcttat cgtcttttaa aagaagctgc cggcgattac ccaaccaaat tcacgattcc
     3421 ggcaccgaca atgttacata ttatctgctg tgtgcgtgcg gaagattacc aaccgttacc
     3481 acaatatcaa gacgaacaaa aactgtacga tgatattgct aatgcttata tcaaagcaat
     3541 gcacaaattc tacgatatgg ggctacgtaa cttacaatta gacgacacca gttggggaca
     3601 attctgtgcc gaagataaac ggaaagagtt tgcagccaga ggatttaatt taactgattt
     3661 agcggaaaaa tatgtcgcat tattaaaccg tattgttgat gcaaaaccgg cggatatgtc
     3721 aattacgatg cacatctgcc gtggtaactt ccgttcttct tggttctcgg aaggcggtta
     3781 tggaccggtt gccgaagtat tattcggtaa atgccgtgtt gacggcttct tcttagaata
     3841 cgataccgaa cgtgcaggca ctttcgagcc actacgccac attaaagatc aacaagtggt
     3901 actaggctta attacctcta aatttggcga tttagaagat aaaaatgtga tcattgagcg
     3961 tattaaagaa gcggctcaaa ttgtgccaat caatcagcta tgcttatcac cacaatgcgg
     4021 ctttgcctcc accgaagaag gcaatatttt aaccgaagcc gcacaatggg cgaaactaaa
     4081 cttaattaaa gaaattgcgg aagaagtttg gggtagctaa tcctctataa tacaaatagc
     4141 cgagtaaata ctcggctatt ttagtttgtt ttaacattac aaccagaaga gatacccaac
     4201 agtcgcaatc acaaagatag agataatatt tagccagaaa ccaactttaa tcatctcact
     4261 ttgtttaatt tgccctgtac caaatacaat cgcattcggt ggggtggcaa ccggcaacat
     4321 aaaggcgcaa gatgctccta gaccaataat taaggctaat ccgaccggtg gcatatttaa
     4381 tgcttgtgct atagagatga aaatcggcac taacaaagcc gcactggctg tgttagaggt
     4441 aacctcagtt aggaaaataa taaatgctgc cacaattaaa ccaattaggt aaaaatgccc
     4501 cccttcaatt agtgagacga ccccatcagc cataattttg ctcgcaccag tttgtcctaa
     4561 tacagcactt aacgtcaaac caccaccaaa taggaataat accccccact cggtgttttc
     4621 ttgaatctgt tgccaatttg cgacacgagt aatgcaaata agtgctgcgg caatcaatgc
     4681 aagaatggtg tcaaagctcg caattttgcc attaattccc agaaatgcag aaataatagg
     4741 gttaatttta tcaccaaaaa tccaaccaag cgcaataaag acgaaaatag ccaaggtgat
     4801 aatacgagaa cgatttaatt caattttctc aaatgatcga tcaaaactaa ctgaaaaatt
     4861 tggtttaaat acgatatata gtgttgcaat cattaatggg aataaaatta gcataattgg
     4921 caaaccatat tttaaccaat cagcaaaagt cagattcaat tgagaagcaa caatcgcatt
     4981 cggtgggctt cccactaaag tacccattcc accaatactg gcactataag caattcctaa
     5041 taacacgaaa agataagtat tatggttttg tttctgatca agtttactta aaatccccat
     5101 tgccaaaggt aacatcattg cagctgtagc tgtattactc atccacattg acaaaaacgc
     5161 tgttaccgca aataaataaa taactgcaat cgataattta cctttagcta atgccataat
     5221 tttattggca atcatacgat ctagctgctg aatgtgtaat gctgtcgcta aggcaaagcc
     5281 tccaaaaaat aagaaaatcg ttggatcagc aaaagcaaca agagcttctt ttgatttaac
     5341 cagccccagt gctacagcca aaatcggaat taataatgcc gtaaccgtaa cgtgtaatgc
     5401 ttctgttagc cataagacgg caatgaaaat taacaatgcc aaccccttat tggcttcagg
     5461 ggtaaaaggc aaggtattta acaaaataat aaataaaata gcatctgcaa caaaaatcaa
     5521 gctattgcgt gtatttttac tcggttcaat aagaggagta ttttccataa aaacctcaca
     5581 aaatatagat aatcagtggt atcactatat ataaaaaaac gatataggca aatataattg
     5641 ttgcaaaagt gtgaagctca tcaaaaaatt taacactaaa aaataaattg cttaaagata
     5701 cagcaccttt gcaaaaataa atcaaaatta caccgcttgt agcgaaaggt attcaatctc
     5761 taacccaaat agtgattttg ccaatgcttg agtcattgca taactactga gagagccttt
     5821 cgcctcagcc agcacaactc gagtaacggg aatatcggca agccctaaaa ataatcgata
     5881 gttcaacgcg ctttgcaacg gtaaaaaagt aggattaaaa gctgcattct cagtatattt
     5941 accacaaaca atacgctcac ctacttgcag tgcaacccca ctaaatgcgt tgctgtaagg
     6001 ggcataagca ttattggcag caaaaattgc cgcttgttgt aaatgatcgc ccttaacctc
     6061 aaattgttgc ggttgttcat caaataagac tttctcaatc cctaaatctt tcggtccaaa
     6121 actatccggc aaatagctat ggagcaaatt attttggcta tgcggtaaat gtacttttaa
     6181 gcttgccgca ctgtttaatt cattcataaa ttggcggcaa tgcccacaag gggtatagtt
     6241 cacaaccaca tctgttatca aagtttcccc tgctaaaaat gcgtgcgaga ttgcactttg
     6301 ctctgcgtga acggattgtt gcatacattc cccggaaaac tcttggtttg ccccaaaata
     6361 gaaatctccg tttttgccaa tcgcaaccgc ccctacatta aaatcggaaa ctggtgtaac
     6421 cgcataacag gcagcaatgg caatgcaata taaagccaac tcaacacgag agcgtttaaa
     6481 tttttcacag agttcatcaa ccacttcgta agagagctgc ccgtgaaaat cttgcttgat
     6541 aagttgctca ataatagctt gtgtaacttc acagtttttc gctgctttta attgttcaaa
     6601 acgctctgta atgtctgttc gaaacatact gtacctccgt ttaatgtttt ttatagctgc
     6661 aaatcagtat ccctgccgta atcagtatgg cactaataat atggtaagta tgaactgatt
     6721 catttaataa aataatactg aacattgctg caaagagcgg cgtaaaatta gtcattaccg
     6781 ctcctttagc agaaccgact attgcaatgc cgaaattcca aaacgcataa gagagaatag
     6841 aagggccgat aaccaaatag cctatcccac tccattgtaa agcggtcaaa tttgctaaat
     6901 tttttgcaga ttctgctaca tattcataga taaaaaatgg cgttaacgtt agaactgcca
     6961 gccccactaa tacggtcaaa aacaccgtat tgcttatacc ttgtggacgt agccgaatca
     7021 cacaacaata aaccgcccag cttatagtag aagcaatcgc ccataaatca ccttgattaa
     7081 ttgccaagct gaataagcga gttagcttcc cttgcatcaa tagccacagc actcccagca
     7141 aactgattat tgccccactt agaataatcg ggctgatttt ctccttaaaa acgagccgat
     7201 ttaaaaatag caccaataca ggcgaaatag ctaaatagag actcgcattc aatgctgttg
     7261 tcgtttttaa accttcatat aaggtagccg ggaataaaat taccccaagc agacctaata
     7321 cccacatcac acctaaacga ggttttaata agtgcattac ccgccaagtt gggcgaaaaa
     7381 agagcaaaac taaaattaaa aaagccggca accaacgcaa ataggttaag gtaataggct
     7441 gaactgcccc acttaacact ttgcctaata caaaattccc accccaaaaa agagtagcaa
     7501 cgattaaact gaaatacccc aatttcagtg atgatgagat cattgatgac ctcgttctac
     7561 aatcacacct acactgcttg cttgggcaac cgctttcggc ttatgcagtt caatccgaat
     7621 ccaagaaagt tgatactctt tttgtaatag ttccgccagt tgataggcaa cagtttcaac
     7681 taatttgaaa ggttgagatt caacaaaatc cagcactttt tgcgatactt ccgcataatt
     7741 caagcaatat tgtacatcat cggtttctgc cgctttggaa aaatcccacg ccatatcaat
     7801 gttaaaaacc aagcgttgct taatcgtatg ctcccaattg taagccccaa tggaagcaaa
     7861 agcggttagt tctcgaataa aaaccttatc actcattttt cgtcctgttt tattttctga
     7921 atttctgtaa aatatagcac aagataaatt taatcaggaa aatttatgaa tttaattccc
     7981 tatttattga tcttaaccgc ctatttgctc ggctcgattt ccagtgcggt cattttttgt
     8041 aagctggcag gcttgcctga cccaagagaa gtcggctcac acaatgccgg tgcaacgaac
     8101 gtactacgaa ttggcggtaa aagtgccgca ttaggtgtgc ttttattcga tattttaaaa
     8161 ggcacattac cggtggcgat ggggatttat tttgaactct ctccctcaca actcggtttc
     8221 atcgccttag cggtttgtct tgggcatatt ttcccgattt tcttccaatt ccgtggcgga
     8281 aaaggcgttg ccaccgcatt tggtgcaatc tccgcattaa gctttattgt tgcaggcggt
     8341 gctatcggca cttggctact ggtgttttta atctccggct attcttcttt aagtgccgtc
     8401 ttcactgcct tggtcgtacc gttttacatt tggtggttcg agccacaatt tactttcccc
     8461 gtcgccttag tttgttgcct attggtttac cgccatcacg ataatattca acgcttatgg
     8521 cgtgggcagg aagacaaact gtgggataaa ttcaagaaaa atagagcata aaaataggca
     8581 tagtgaaccg ttcgctatgc ctatttgcaa attattgcgg aaaaatcacc gcttgtcttc
     8641 tcgactaaac cgctaaatct gcttcatctc ctcgattttg tagccattgt ttacgatctt
     8701 ccgctcgttt tttacccaat agcatatcca taatttcaaa ggtattcaat tcgccctctt
     8761 ctaccgtgtc atcaaaggtt aattgtacta agcggcgagt gcttggctcc atcgtagttt
     8821 cacgcaactg gctcgggttc atttcgccta agcctttaaa gcgttgcaca tttggctttc
     8881 cttttttctt ggcaaggcga gctaaaatcg cctctttttc cgcctcatct aaggcataat
     8941 gcacctcatc tttgccgata tcaatgcgat aaagtggtgg cattgccaca taaacgtgtc
     9001 cgttttgcac caatttcggg aaatgacgaa ggaataacgc acacagcaag gtggcaatat
     9061 gcaaaccgtc tgaatccgca tcggcaagaa tacaaacttt gccataacgc aactcttcta
     9121 aattatcgct atcagggtca attcccaagg ctaccgcaat atcgtgtact tcttgtgaag
     9181 ccaatacttg gtctgatgaa acctcccaag tgtttaaaat cttaccacgc agtggcaaaa
     9241 tggcttgata ctctttatct cgagcttgtt ttgccgaacc gccggcagaa tcaccttcta
     9301 ccaagaaaag ttcggtacgg ctgagatctt gagctgtaca atccgccagt ttgcccggta
     9361 aagccggacc actcaccaat ttcttacgca ccactttttt cgccgcacgc atacggctct
     9421 gggcggaact aattgccatt tcagcaatta atttacctgt ctgcacattt tggttaagcc
     9481 ataaactaaa ggcatctttc aaggtgctat ccacatagct tgaggcttga cgggaagaca
     9541 aacgctcttt ggtctgccct gcaaattgtg gctcttgaat tttcagagaa agcacataag
     9601 cacaacggtt ccacacatca tcagcggtta attttacccc tttcggcaat aaattatgga
     9661 tttcgcaaaa ctccaccatc gcctttaata aaccgttacg caagccatta acgtgcgtgc
     9721 cgccttgtgc ggtcggaatc aggttcacat agctttccgc tactaactcg cccccttccg
     9781 gcaaccaagt taatgcccag cttaccgcct ctgtttcagc agtaacatca ccaataaacg
     9841 gtggattcgg cacggtttca taaccgttta atgcctcgct caaataatca gataagccgt
     9901 cttcgtaata ccaactttct tcggtattgt tgattttatc aataaaattg atggtgagtt
     9961 tcgggcaaag caccgctttg gctcgtaaca aatggcgtaa acgactcacc gaaaaacgag
    10021 ggctatcaaa atattttggg ttcgggtaaa aacgcactgt cgtgccggtc tgttttttcg
    10081 ggcaagtacc aattaccgtc agatcttcca cttttacccc gttggcaaag gcaatcgaat
    10141 agacttctcc gccacgttta atctgaattt caacccgttc ggacagggca tttaccaccg
    10201 aaatccccac cccgtgcaaa ccaccggaaa acgtatagtt tttattggaa aatttaccgc
    10261 cggcgtgcaa tttcgttaag ataagctcca cccctgaaat tttctcggtg gaatgaatat
    10321 cgaccggcat tccccgcccg ttatccacca cttccaacga attatcagca tgaagaatca
    10381 catcaatttt atctgcaaag ccggacaacg cctcatcgac actattatca atcacctcct
    10441 gccccaaatg gtttggacgt gtggtatccg tgtacattcc cgggcgaagc tgcaccggct
    10501 cgaggtcttt cagaacggta atttcatccg ccccatagcg tttttcagac attatgcttt
    10561 ctcttaggct taaaataaaa taaggctgaa ttttaaggaa tattcagcta ttttgcaaaa
    10621 ttttggtgaa aaatgacctg ttgttatgca ataaaaacta tttttgcgat ctacatcgaa
    10681 aaaatgagtt ttaatttccc tatttgctta ttcattcagt atcttaatct cactttttat
    10741 tttaaaatgg tactatatta tggtagtaaa aatcctaaag cggaaatctc aaaagacact
    10801 acaacaaatc tacacccagc ctatttctgc caatattaaa tggacaaata tagaagctct
    10861 ttttctagag ttaggagcag aaatagaaga acgagaaggg agcagaattg cagttgtttt
    10921 atttcaacag gtcagagtat ttcatcgccc acacccgaat cctgatacag ataaggggcg
    10981 gtagcgagta ttaaaaaatg gttatctgaa aacggagtca ttccttatgc gtaatattat
    11041 ggatattaat ggttataaag ctgtcattgc ttacgatccc gaaacagaat tatttcgagg
    11101 tgaatttatc ggattaaatg gtggagccga tttttatgcc gataatgtca ttcaattaaa
    11161 aaaagaaggt gaactaagtt tgaatatctt tttagaactt tgtagagaaa aaggtattga
    11221 gccgttcaaa caatattccg gaaagtttaa tgttcgcctt tcgcctgaat tacataaagc
    11281 tgcggtaatt gcagcaactg ctgaaaattt aagcttaaat gaatggatta accaaacgtt
    11341 agaaaagagc gtgtagccta attgcaaaac aatcacctat tttgaccgct tgtattaccc
    11401 gtttaaccca ttcaatatcg aacactccga attggcgttg ccacagcaag agctactcca
    11461 gcttttgagc aaagtcagca tttggtttaa atcggcaatt ttttgttcaa gctcaccgat
    11521 atgctgttcg gttaattgtt ttacatctcg gcttgagcgg tttgggttgt cgtttaattt
    11581 aagtagttcg ctgatttgag ccagcgaaaa gcccactttt cgggcattgc tgataaaatg
    11641 taatcggctc aaatcagtag cattgtagaa tcgatacccc gcttcggtgc gacttgtcgg
    11701 cggcagaagt cctgcttttt cataatctcg gatttgtttg gcggaaagcc cggttttttc
    11761 tgcggcttgg ctaatattca ttataatcct ctcactttaa tcgcttgacc ttaacctaag
    11821 gttaagcttt attatacgcc tgaataatca ctcaaaacag gagaaaatga tgacaattac
    11881 cctacaactt gatggcttac attgtggcaa ctgtgtgaaa agtgtcgaaa aagcgttaaa
    11941 tggcgtggct ggggtgaacc aagttagcgt aacgttagaa ccgcaaattg ctgtggtgga
    12001 aggctcagca gaagcacaag ccttaattga agcggttgaa gacattggct ttgacgcaaa
    12061 attagcctaa ttaataaggg gcagaaattt tgccctaatc tgcccaatat ttcggaggat
    12121 aaatgacaag cacagctcac tattcgccag aacaacaact tttgattgat ggaatgcact
    12181 gtgccgcctg tgttcggcga gtggaaaaag ccttacttaa agtggaaaag gtggaatttg
    12241 cctcagtcaa tcttgccgat caaaccgctt ttgtgcaagg caacgccaac cctgaagcct
    12301 tggtagcagc ggtagttaaa atcggttttg gggcagaagt gctggaaagc gaggaagaac
    12361 gccgaattaa acaacaagcc caaacgcaac gagtgttagg gcataaacgc actcaattta
    12421 tgcttgccct tgtagttggt tttgccttag ttgcttttgg gttctacaaa ggaatgacgg
    12481 taaacgaaac caatcgttcg ctttggctca gctgggcagg tattagcctt gcgacaatgt
    12541 atttttcagg taaagatttc tttcttgggg cattttcagc actgaaaaac cgctcagcca
    12601 atatggacat gttagtagca atcagcaccg gcacggcttg gctctattct tgttggctca
    12661 cgcttttccc acaaccgaat gctcacgttt attttgaagc cagcgtgatg attctcggct
    12721 ttatcaatct aggaaaatac ttagaactga aagccaaaca gcgttcctcg ctagcattgg
    12781 aaaaattgct ggatctcgcc ccaaaacaag cggtcatttt tgaagaaaat attgcaaaaa
    12841 ccattccggc taaagcaatt aagccacaaa tgcgggtgca agccttaacc ggtgaccgct
    12901 tggcggttga tggtatttta gacagtggct ctatttgggt tgatgaatca atgctcaccg
    12961 gtgaagccct acctgtagag aaaaaaatcg gtgataaagt gcgagccggc actctggtgc
    13021 aagacggttc aggcacttac attgccgaac aggtcggctc acaaaccgct ttggctcgtg
    13081 taatcaatgc cgttcgccac gcccaaagca gcaaaccgcc attggcacag tttgtagata
    13141 aaatcgccgc tgtttttgtg ccggtggtgg tttcaatcgc attactttct gcgttaattt
    13201 ggctggcagt tgggcaggat tttgcctttg cactctcgat ttttactacc gtgttgatca
    13261 tcgcttgccc ttgtgcattg gggctggcaa ttccgctttc taccattgcc ggcgtggcac
    13321 gttctgccga atttggcgtg ctggtgcgaa atattgaggc actacaagca agcagtgaaa
    13381 tcgacaccct agtgttcgat aaaaccggca cactcaccac tggcagaatg gaagtgtcgg
    13441 atgttctcac ctttaacgga tttgagcaaa agcgcttgct acaacttgct aaaagtgtag
    13501 agcaacacgc ctcacacccg attgcgaaag cgattgcaaa atttgccgaa aatcagaccg
    13561 cttgtggtgt aaccgatgtg caagtgttga aagggttagg catttcagca aaatatgaag
    13621 gcagtacaat cagaatggga aattggcaat ttgtaggcga aaccattcaa gcaaatcttg
    13681 cctccctatt gcccgctaaa gagggggatt tttcctcaaa aatttttgtt tcggtggata
    13741 atcaactcgc cggtgtattg tcggtttcag accaacttcg ccctgaagcg aaagcaacca
    13801 ttcatcaatt ccaagcggaa ggctatcaat gcgtgatgct gaccggtgat cgccaacaaa
    13861 ctgccgacta tttcgccaac gcattggggt tagacggcgt gattgcggag gtattaccgg
    13921 agcaaaaagc ggaaaaaatt cgagccttgc aagcagaggg taaaaaagta gcaatgattg
    13981 gcgacggcat aaacgatgct ccagctctgg ctcaagccaa tgtgggcatt gcgatgcaca
    14041 atggttcaga tattgcagtg gaaaccgccg atctttcgct gatgcgagaa gggctagcac
    14101 cactgatgca aattctaccg ttctctaaaa aagtgctgaa aaatatgaag caaagtttgc
    14161 tcggggcttt tatctataat gttattgcga ttcctattgc tgccggcgtg ctttaccctt
    14221 tcaccggctg gctactcaac ccaatgattg ccgccattgc gatgacattg tcgtcaatta
    14281 ccgtggtgtt aaatagtcaa aggttgttga aataatttaa gcggtcaaat ttgcatattt
    14341 ttttgcaaat ttgaccgctt gtaatcacta atccgccaaa aacagcggcc caagaggtgt
    14401 tttcggaatg ccaaaatgct cagggtaatg cacctcgacc aaatatagcc cctcggcttt
    14461 cgctgtaggg gcagcttttt cccggtcacg ttgctccaac acccattttg cccattccac
    14521 aggctgtctg ccttgcccga tttcgagcaa tgttcccaca atattgcgaa ccatatgatg
    14581 tacaaaggcg ttggcttgaa tatcaacaat cacataattg ccttgccgag acacatttaa
    14641 atgatgaata ttccgccacg gcgtattaga ttggcatttg gcggcacgga aagaggaaaa
    14701 gtcattctcg cccagcaaat actgccctgc ttggtgcatt ttgcgttcat caaggggaaa
    14761 gtgaaagtgg gtaacacctt taggtaaaat tgccgaccgc agtttttggt taaaaataat
    14821 atagcggtaa cggcgggcgg tggcactaaa acgggcgtga aaatcctcat tcaccgctac
    14881 cgcccatttc accgcaatat catcgggtaa atgagcgtta gtgccgaaac accagctttg
    14941 tagcgggcgg tttgcttgcg tttcaaaatg cacgacctgc cctgtgccgt gcacaccggc
    15001 atcggttctg ccggcacaaa acacttctac gggcgaattg gcaacaaccg acaacgcctg
    15061 ctctagctgt tgttgtacac tctctaccgc ttcttgtcgt tgccagccaa aataacggct
    15121 gccgtcatat tcaataccta atgcgatttt cattttaata ccttgataaa aagaggggcg
    15181 aaaaattttt cgcccccaca gtaattgata tttgcaatta tttattcaat aagcctaaaa
    15241 attctttgcg ggtttctcgg tcttctaaga aaacaccgcc aaaggcagaa gtaactgtat
    15301 agctattggt gtcttttatg ccacggcatt tcacgcaaaa atgggtggct ttcacataca
    15361 ccgcaacatc gtctgtttct aaaatagttt gaaaggcagt taaaatctgt tcggtaaaac
    15421 gctcttgcac ctgcggacgt tgggcaaaaa actgcacgac acggttgatt ttcgagagtc
    15481 caatcaccca ttttttcggg taataggcta ccgctacttt gccgtcaata gtaacaaaat
    15541 gatgctcaca ggtagaagtt aaagtaatat cgtccaccag caccatttca ctgactttca
    15601 ttcggttttc aatattggta attttcggga aggtagcata atcaagcccg ctgaaaattt
    15661 catcaacata cattttcgcc aaacgatgag gcgtttcttc taagctgtca tcacttaaat
    15721 ctaaaccgag caattctaaa actgcctgca tatgctgctg gatttctgct cggcgactgg
    15781 ctttatcttt ggtatgagta acagtcggag tttcaatgcc ttttgccaac aaggcttggc
    15841 gaactttttg agcctccggc gaaagcgtgt tttctgtgat agttaattcg ggcgagatcg
    15901 ttgtcattgg ttttcctctc aaatgtaagc tgaagattct aacaaattga catcaaaaaa
    15961 aatagctcta aaatgagcta tttcagccat aaacaccctc tttatttatg caaaatatac
    16021 agtatctctt taatgccgac ctgattcccg aacatcaagc tgaatttgtg cagtctctct
    16081 taacttgcaa aaattttgac aattccgacc gcttgctagg cttctcaaaa gggcgaggta
    16141 cgacttggtt tttagatacc tctgctgaac taggtatcaa tgtggtgctt cgccattatt
    16201 atcgtggcgg cttgttcggt aaaatcatca aagatagtta cttttttact cgctttgagg
    16261 ccacccgagc gtataaagaa tttttcttac tgcaacaaat gttagcttgg ggtttacctg
    16321 tgcctcgccc tgtggcgatt aaaatagaaa gacatttttg ttgctatcgt gccgacatta
    16381 tgctcgaaaa gctagaaaac acacaagatt taagcaaaat cttgcaatcc cattctttat
    16441 cgaacgaaca atatgagcag atagggaaac tgattcgcag tctgcataac catcaaattc
    16501 accattccga cttaaatatc cataatattt tgcttggtaa tgatggcaaa ttttgtctga
    16561 ttgattttga taaatgcggc attgagccgg gcgaaaattg gaagcaaaat aacctagacc
    16621 gcctgttacg ctcgtttaag aaagaacaag ggcgattaaa tattttcttc agcgaagaaa
    16681 attggcaggc tgttttgaga ggatatactc tctaagaaac aataatggca caagcttaga
    16741 aacttgtgcc atcaaatcat caatattaac tacttcactt cacgaatcat aatttcagac
    16801 ggaatcacac tgccttgcca gtaaagttca gaagctactt tttccgctaa tgcgaggtag
    16861 gcttggctga tttcgtgttc cggagctgcc accaccgttg gcacaccgct atctaaatct
    16921 tcacgcagac gaatatgtag tggtaattgt gctaacactt tagtgccata tttttgggcg
    16981 attttttccg ctccgccggt gccgaaaatt gcctcatggt gtccgcagtt tgagcagata
    17041 tgcattgaca tattttcaat caccccaagc actggtactg agactttctc aaacattgag
    17101 atccctttaa tcgcatctaa taaggcaata tcttgtgggg ttgttactac caccgcaccg
    17161 gtcaccggaa tttgctgtga aagcgtaagc tgaatatcac ccgtacccgg tggcatatca
    17221 atgaccaagt aatccaattc attccaccaa gtttcattta aaagctgact taacgcactg
    17281 cttgccatcg ggccacgcca aatggtggcg ttatcttctt ccattaaata gccgatagaa
    17341 ttagattgca agccgtgagc ggtaatcggc gtgatgtgct tgttatcagg agaagttgga
    17401 cgttggtctt ttgctcccaa catatgcgga atagatgggc cgtaaatatc cgcatctaaa
    17461 atccccactc ttgtcccttg tgctcgtaag gctaaagcaa gattaactga ggtcgttgat
    17521 ttccctaccc caccttttcc tgaagtgacc gcaatgatat tttttacccc attgactgcc
    17581 gggtgtttat tcgcccgttt taaggtcgca atttggtaat ttaataccca ttttacaccg
    17641 cttgaacctg ttacttgctt taatttagcc tctgtttcgg cttttaaggt ttcaaaacca
    17701 ctgttccacg caaatggcat cgtgatttca attctaagaa tatcgccccc taattctgct
    17761 tttttaaacg cattaagggc aattaaatct ttctgtaaag tcgggtggga aaattgttgc
    17821 aagactgctt taatttcaac aagttgctgt tcatttaatt ggttcattgg ttatccttgt
    17881 ttgctaaatt gcgttttaaa tctttgctat aaattacttc gctatcgtta ttcaaatcaa
    17941 tacccaaaag ggaggaaaca atcccaaccc gatgatggta ttgaaataat ggcaatacgg
    18001 caacatcgct ataaagctgc tcgctaattt cttgaataag tgactctctc gcttctgcac
    18061 taagcgtttc tgtttgtaaa cgatttaatt tttgatcgac tatctcactt ttatagccgg
    18121 aatgattatc cgggctcatt gaatgaaact tctgcaaaaa tacaatcgga tcaggataat
    18181 ctgcacacca gcctgctcgt gctatctggt aatctttttg ttcccttact ttatttaaag
    18241 tctgccaatc aaccatttta ggtgtaatat taaataattc tgattgagaa agggctcgag
    18301 taacttggtt agcaatatta atgctattct catcatataa caaagtcagc tttaaaggat
    18361 tataagggct aattcctgct tttgttaata gctgctcaac cacaacagga ttccaacgct
    18421 tttctctatc ttttactaag ctatgtggaa ttacagactg actcggaacc ccatattctt
    18481 gcccactatt tgctgaagcc accattgtcc ttattgcttc acgaatctct cgtttagcta
    18541 ataatggatc atcaaaattg aactcatagt aataagagca caaacgcgga aatttcacga
    18601 tattcgtttt tttatttaat aaaggattct cgacaatatc aaaagcagag cagttttgga
    18661 tagttgtaat taaatcatac tctatagttt gaaagggaat ttcttgggtt cgagccttta
    18721 acaccatttt ttgagcatct agtttctcga gacggtaaga cgcattgctc acgaagttat
    18781 ctatggttgg agcaatccct tgataagtag gtaataatgc ggaatgtgcc agcatttcag
    18841 gcaattggtg atttgggtga tttagccgaa ttctcaatgt atgcgcattt aatgcctcta
    18901 caccgagttc ttcaactgac tttatttgct cttcaatcgc ttttgcattt tctataccca
    18961 tatagatgag atatttagac aatacagaat gattttgagg atctattaaa cgtcgccagc
    19021 ttgctacaaa atcttgtgca gtcacaggtt ggccattaga ccacacagct ttctcatcta
    19081 aaataaacaa ccactctttt tcatcttcag taaaccaatt ctgagcaatg gccggaacga
    19141 catcaccttt tctgttgtag gccgttaaac cgactaatag atctcttaaa ggagcagcat
    19201 ctgcagaagc tttaatttta tgtgggtcga gttgaaatac agcagaataa attgcacgtt
    19261 tgagtaaagt atcactctgc cggttttcta cccagacaga ttgaatggat tgagggcaaa
    19321 ttaattgatc acaagatgta ataagcagaa tgcaagcggt aacttttgca atttttttta
    19381 caaatgtaac cgcttgtaag gatttcaaca taaactcccc aaaggttaat taagatttat
    19441 tttacaagaa gcacaggaaa taaagttgta attcctgcca caataatttc aattcccagc
    19501 gccattaaaa tcagccccat gatacgagta acgacattag atcctgtttt gcctaatttt
    19561 ttcaccaaag gggctgaata gcggaataaa atataacaaa ttaatgcaaa tacaataatc
    19621 gcaatgctaa agccaacata gtcttcccaa gtatggtagc gggttcccca cacaatggtt
    19681 gagccaattg cccctggtcc tgccataatc ggcattgcta atggcacgac accgatattt
    19741 tcgtattcag taatatcttc atttttctct tctttatttt gtttgtgttc acctaattta
    19801 ccgctaatca tcgtcatcgc aatgcttaca atcaaaatgc caccagctac tctaaaagag
    19861 ctaagtgaaa tgctaaacgc attgagaatc agattaccaa aatagaggct gactaataaa
    19921 attactccaa tagaaacagc ggtgattaag ctggtgcgat ttctttcccc ctcatattgg
    19981 tgagcggtca tactgaaaaa aataggcaag gaaccaaaag gattgactat agcaaataga
    20041 ccaatgaaga attggaggta gatggcaaaa ttaataatga tatccacaaa aactttctca
    20101 aacaacagtg tttcgggcgc tattatatcg gttaatcaag gtagatacaa accaaaagca
    20161 cccagcctaa aggacggttg ggtgcttttt gataaaatga aaggcttgtt agtgtgacga
    20221 acgttgctgc ataaaatctt catttacctc aattgaagct tttgcacgta gcgattgtaa
    20281 gaattcatta cgtaaaatta aacgattagc ttgttcaaat tgtggcgcca atgtcttaaa
    20341 ctgttcagca ttcccttccg taatgttatc taaggcaaca ataactacat cgcccttttg
    20401 attacgtgcc atttgataag tcgttttatc ttgcgccttt ttcattgcga aaatcgttcc
    20461 agccaatacc gggttttcta cttctgcttg catataaact aaagtttgtg gcgcattaaa
    20521 tttcacgtcc actgatttcc cttcattcaa ggcttttaca ttttcttcta ctttcgccag
    20581 caacgctttt tccgcttttt ctgccttcac aaaattttca actgctgctt ttgcttcatc
    20641 aaaagtttgt ggacgttctg catcataatc actcactctc acaaacaatg tctgtggatt
    20701 tgcatcattc ccaacatcta aagcatcaga attttgttta ttttggcgta gctctccttc
    20761 aaacaaaact tttaatactt tctcattttg taaagcagta ggtacgtttt cacgggtaaa
    20821 cagatccgtt ttttgcagtt ccacattacc ggctttcgca acttcttcaa gtgaagaatt
    20881 attctcaaag gctttatttg ccatttcgcg cgcaactgta aaatactcag ttaacactaa
    20941 ttcttggcga atagtttggg caattttatc tttcacttgt gctaactcaa tcacttgctc
    21001 aggtttacgc tccaatactt tgatgatatg gtaagcatta tccactttca ccgctgcact
    21061 catctggccg gcagttaaat ttgctacagc atcttcaaaa gctttcggga aagtgccagc
    21121 ttttgcccaa cctagatcac caccattatt tgctgataaa gtatcgctag atttgttttt
    21181 tgccaaagta gcaaaatctt ccccattttt gagcgcttgc tcaatagcct gtgcttccgc
    21241 ttcactagcg acttgaatat gagctaaatg ggcttcacct ttagttacat aatcggcttt
    21301 attgcgatca tagtaagttt gaatttgctc attggtaatc tcaactttgc tttccacatc
    21361 ttttggagaa agagtaacgt actcaaccgc taatttttca ggtgctaata atttagtttt
    21421 atttgcttcg taataggctc gcaattcttg ctcggaagcg gtctgatttt ccatttcttt
    21481 tgcaactgaa aattcggcta aacgcacttc acgttgttgc aacagtaatt ttgctaacaa
    21541 tgcctgctgt gccggtacat taaaatcact attgataatc ccctcttgta gctgggattg
    21601 caacatacct tcctgcacaa tcgctgcata gccatcggca cttaaattat tgtggcgtaa
    21661 tgtttgttgg tataactcgt tactgaattt accatcttgt tgaaacatct ggctgtttac
    21721 aatttcagat ttgatttgat cagcactaat acctagtttt aactcttttg catactggcg
    21781 cagaagctct tcatcaatta atccatttaa taccgattga ttaaattgag cagcatactc
    21841 cgggctatcc attaaatccc aaaagcgact ccccagttca gcatttaatc tattttgctc
    21901 acgattaagc gtgttattaa ataattgctg actaatttct tccccattta ctttcgctac
    21961 agaattatct tgtgaaatca atcctgtccc aattccaccg attacaaatg aaatagagac
    22021 taatgcaaaa ataattttaa agaccggccc atttgtccgc tcgtgcattt tttcaatcat
    22081 ttaaaaattc ctaaattact caaaaattgt cgctgattat actctttctc aaatgagaaa
    22141 taaacttgca aatttttatt gaaatttaac cgctttctct ttaaaattca cagtccctgt
    22201 cgtagctgag ttagagatcg gctattgatg tattatcaac ccttaacaaa aggaaattaa
    22261 aatgtctcta agcgtagaag caaaagcaaa aatcgttgct gaatttggtc gtgatgcaaa
    22321 agacactggt tcttctgaag ttcaaattgc attattaact gcacaaatca accacttaca
    22381 agcacacttt gctgagcata aaaaagatca ccacggtcgt cgtggtttat tacgtatggt
    22441 ttcacgccgt cgtaaattat tagactactt aaaacgtact gatcttgcta aatattcgga
    22501 aactatcgca cgtttaggtt tacgtcgcta atttcaaata ttagatatga gagccgagct
    22561 gaaaagttcg gcttttttat aaattttatt cgaggaaaaa atatggcttt tgcgtcatta
    22621 tttacattat tagacgatat tgctgccgtg cttgatgatg tatcgatcat gactaaagtg
    22681 gctgctaaga aaacagcagg cgtaatcggt gatgatcttg ccttaaatgc taatcaagtc
    22741 agtggaaaga atgtggtgcc agaacgagaa ctaccgattg tttgggctgt agcgaaaggc
    22801 tcattaatca ataaagtgat cttgattcca ctagcgttgc tgctatcggt ctttttaccg
    22861 caggctatta tcccgctatt aatgataggc ggtgcatatt tatgttttga aggcgtagag
    22921 aaattactcc atcgtctatt acataaaaac aatcaagctc attctgatga aaccgaaacc
    22981 ctttctgaaa aagataaaat taaaggtgca attcgcaccg attttattct atcagcagag
    23041 attatcatta ttgcgttagg tgttttgcaa gactcttcaa ccctaaccca aattttatct
    23101 ctctccgcta tcggggtcgg aattaccctg tttgtttatg gcttagttgg attaattgta
    23161 aaactagatg atatcggttt atggttaatg cagaaaaaaa gcacattctc acaagcggtt
    23221 ggaaaaggct tattatttgc aatgccgtgg tttatgaaag gactttctgt gattggtact
    23281 attgcgatgt tcttagtcgg tggtggtatt tttacccata atttaccaat gattcatcac
    23341 ttcgtagaac aattcggtat ctctccccat ttttctactc tggttgattt tgcagtgggt
    23401 attgtggttg gtattattgc atgcttaatt attttacctt tgatgaaatt attttctaaa
    23461 aaggcgcact aatccccaaa agcgtagctt tggttaaagc tagcttttta tctcttgcta
    23521 taaaaaaata gcaggtttca agcctgctat ttttttaatt attataatga gttagactca
    23581 gtggctgttt gcggtaaggc taatggctca ttaactttag actgagtagc catattcacc
    23641 tgtttgagat tttcctcacg aatacggttt gccattaatg tatcccccat ctgctctgcc
    23701 acgtgcagtg ccataatacg atcatcagca acacattcat catgagccaa taacaattca
    23761 aactgagact tcgctttttg gtattgccca tctcgtgtat aaatatagcc aagcgctcta
    23821 gcataatcat cggtatattt aacattcgct ttatctgccc gttttgctaa tacttttagt
    23881 agcttactgt cagaatcaat ttgtaaacgg gttaattgca caaataaacc ttgtaattgc
    23941 tcatcttcaa atttcttcaa cgtttctaaa gcaatttctt gtgcagattg atgatcgtca
    24001 ctatcaatta aacgcttgat gacaccgaca cgactataca ccgatttacg acgacgattt
    24061 ggttggtttt cccaccaaga aagcaagcct tcctgccctt cttcctgtaa acgctcatct
    24121 tgtaaaccat catcaacgaa atactctaac tcagcatact cttctgcagt taagaaatta
    24181 cgttggccga tttcaactaa taatctatct aacgcaacat aagctctgga ttctttataa
    24241 atttgaatcg ctaagcgtaa tacctcatcg ctatgaggtg caagctctag taggctatca
    24301 atcgcagttc gggcagccgg taattttcct tgttgtaata aaattcgggt tcgtaccaac
    24361 tccactgcaa cattattcgg tccagctatt tcagttgcct taattaaata tttatttgca
    24421 gctaaatcat caccattttg ctgagcagct tccgccgctt taattaaatt cagtacaggc
    24481 tcatctgcat gttttgcatt tttactaaag agtttttccg cttttgagta attcccttca
    24541 ctcatacgca ttaaaccttc cagtgtttct tgctgagctt ttttatgctt acgagttgag
    24601 aaccagttat aagctccatt actcatacgg catattttag tgacaatcca ttcaagtaaa
    24661 taaactaccg ccattgccaa cacaaaaaaa accacaagca ttacaatgct catttcaatc
    24721 acggtagttg ctgtttcaat tctgacataa ccttgatttc cggctaaata aggcccagag
    24781 attagtccaa ctaataataa aagcatcaaa aagagcgttc taaacataat ctattctcct
    24841 ttttattatt gtgctgaaag aggcatcgca tcttgctctg cttttacagg ctctgcttgc
    24901 tctaattctt tttcagcttt tatttcgact ttctctattt tttcaggggt tttattaagt
    24961 tgctgagcaa tcacctttaa gctttgtaat gaagttggag catccacata aatagtttgt
    25021 tctgccaact catctagctc tttcaagaaa tgttgcacat tttcatttga agtttcaaaa
    25081 taacttctta cccaagagcc taccgtttgt aacgattttt tataaagttc gctttgctgg
    25141 cgaggaacag ctaaaatcgc aatttgtaaa cgcaagcgaa tgttttcacg caaataaatt
    25201 tcttgattcg gtgcaacaaa ggctttctca tctgctgcat tacgtttcga tacacgaata
    25261 aaatgatcta aaaatgaagc agcacttttt tctaaatttt tctcccaatc cgcaagagaa
    25321 tcacttactt gcccatcttg tttgctattt gaagcttgtt cactttccaa aatcggcaaa
    25381 tcatctaatt tattcgctaa atttgcgaca cgctgcatta aggcattttg atcgatctga
    25441 tgcaatgcgg ttaatgtatt taaatcagtt ttgattgcct cacgaatgtg aatcgtattc
    25501 ggtgcttttg ataaaacttg atctgcctct aacaataaat ttttcgctgt ttcaatgtca
    25561 ttatctaaca ccactttacg agccgcatta ttcaatagga aatctgcctc agataatagc
    25621 cagaatgtgt tatccgaacc agaggtatta tttagctttt gcagttgaag ctgcaaacta
    25681 ctaatttgct gggtataacc attttgggct gtttctaaag ctttaatccg ttctaaagct
    25741 ttttgatatt ccgtgaccaa ttcggcaatt tgtgcttttt cattttcgaa atttggcatc
    25801 tcaaccatag tttgagtagt aggctgattt gctttagcag atactgcctg aatttgagat
    25861 tcaacctctg caaacttttt atttgccata taatgaccag ctccgccaac cgctaaagcc
    25921 actaatagtg caactaatgc caagcccttt ccgcccgatt tttgaacaac cacttgttct
    25981 tttggcgctt tttgcatttc tgtttgaata tctttctcta tagcttcgtt cactacatct
    26041 tcctcaatct ctttttttgc aaaagatccc gctgaatcga ccgcttgttg tgcctcttct
    26101 accacctttt cagctgaatg ctctaaatgt tcagttactt ttttagatct tgacatactc
    26161 gccacctcta gttagatttg cccactttaa ataataatgt attcatcaaa gttacattat
    26221 cggctttatc cgatacccta atcttatctt cttgccagcc catttgttta gctatctttg
    26281 caatccgccg gctcactaca accagatcac attcaaataa ccatgctcta caatctgctt
    26341 tcgcttgttc ataaagtgat cttaaaattt cactactggt gatcacaata gtatctactc
    26401 ccaagcgttt acataagctg attttttcgg gaatatcatc ggtaaccggc tctctacgat
    26461 aacactccaa atattgaata gttgccccac gcaagactgc agtttcagca agtagctcac
    26521 gcccagaatc ggctcttaga attaaaatag ttttatcagt tagattctgc atttcaggta
    26581 atgccaaaac cccctcgcta ttttcaaatt ctatcggata aatgaccgct tgttcagctt
    26641 tttctgttaa atatttcgca gtccgttgcc ctaccgcaaa atatttcaaa tctgaacgaa
    26701 aatggaaccc ggtatctact aatgtttttg cagcatagtc cactgcattc ggcgaaacaa
    26761 caaatacata atcgtctgat tttagccgag aaatagccga aggaagcaaa ggtaattctt
    26821 taccggcttc aatcgtaaat agcggttgat gaatagcaaa aatttgctgt tcattcaaca
    26881 tatctaccaa ttcttgcccc cgactatcca ggcgtgtgac caacacattc attattgtac
    26941 gttttcgtta taaaccgcag ctaaaatttt atctgcacct tgagcaagca actgttccgc
    27001 caccaaaatg cctaattgct ccgcctcttg cttatgtcct tttgccgaag cccgaataat
    27061 ttgtgagcca tctaacgatc caactaacgc attaagcgtt aattcatcgc cctctaatgt
    27121 agcataacca ccaatcggca cttggcaacc gccttgcaaa cgggtattca ttgcacgttc
    27181 tgccatcacg caacaagcgg tcgaactatg gtttaatttt gcaatgtagt tcaaaatccg
    27241 ttcatcattg gctcgagtct caatccctac tgctccttga ccagctgcgg gtaatgattg
    27301 ttccacagaa atatagctac ggattcgctc ttgcaagcct aaacgaatta agccggcaga
    27361 ggctaaaata atcgcatcat actcgccatt atctaatttg cttaagcgag tgccgacatt
    27421 gcctcgcaat gatttaacct ctaaatgcgg atatttcgcc attaattggc actgacggcg
    27481 taagctcgat gtaccgacca cagcaccttt cggcaaatct tctaacgaat ggtagttgtt
    27541 cgacacaaac gcatcacggg gatcttcacg ttcacaaatc accgcaagcc ccaagccttc
    27601 gggaaagctc atcggcacat ctttcattga atgcaccgca atatcagctc gattttctaa
    27661 aagggctaat tctaactctt tcacaaacaa gccttttccg ccaattttgg ctaatggggt
    27721 gtctaaaata atatcgcctt tcgttaccat cgtaaccagt tcgacagata attgcggaaa
    27781 atgtttttct aattcgcttt ttacaaaatt tgcctgccat aaggctaacg ggctttgacg
    27841 ggttgcaatg cgtaaaatat ctttcataaa ttctaaaatt aaaacattga gttaatgaat
    27901 tatcctacca ctttttagct gaactgccaa aaataatcgt ggatttcgct ttgcatttaa
    27961 tcaaaaatcc gactttttct caaaatgtat caa
//
LOCUS       DANLUQ010000013        27431 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.013, whole genome shotgun sequence.
ACCESSION   DANLUQ010000013 DANLUQ010000000
VERSION     DANLUQ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 27431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27431
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.013"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            74..1300
                     /locus_tag="PXB27_000470"
     CDS             74..1300
                     /locus_tag="PXB27_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HDL5345297.1"
                     /translation="MAMPSQRFSLTWVLNLFGTAVGAGVLFLPINAGMGGFYPLIIMT
                     LLVGPMTYLAHRGLTRFVLSSKYKGSDITAVVREHFGEQAGKLITLLYFFAIFPILLI
                     YGVGITNTVSSFMENQLHIAPPSRAVLSFMLIAAMIGVMLLSEQVMLKITTCLVYPLV
                     LILLGLSIYLIPQWNTAALQQMPTTGDFLTTLWLTIPVLVFAFNHSPAISSFALSQQK
                     YYQDDKKAEIESAKVLRSTAFILVLFVMFFVFSCVLTLTPEELAQAKVQNISILSYLA
                     NKFDNPIISYFGPLVAFLAIGSSFFGHYLGAREGLEGLVNQMRKEPIDPSKFRKITAI
                     TFLIILWIVATINPSILGFIESLGGPIIAMILFIMPVYAVYKVPALARFKGEFGHLFV
                     LVMGCIAISAIVYGLL"
     gene            1357..2733
                     /locus_tag="PXB27_000471"
     CDS             1357..2733
                     /locus_tag="PXB27_000471"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDL5345298.1"
                     /translation="MISVFDMFKVGIGPSSSHTAGPMKAAKEFVDELQNRKFLNKIDR
                     LRVDVYGSLSLTGRGHNTDIAIIMGLMGYLPHNVDIERIEQTIENLKQNKQLIIAESS
                     VEPKVIAFDFYEDMPFHRDFLPRHENGVKFSALSGEELLFQNTYYSIGGGFIVDEANF
                     EQVKNSDLSVPFPYQNAADLLAHCKNNGLPLSSLVWKNELALRSRVEISDYLAMIWNT
                     MEACIERGLNTEGLLPGPLKVVRRASGLRRMLEANSKFNTDPMQIIDWVNMFALAVNE
                     ENAAGGRVVTAPTNGACGIVPAVLAYYQKFIAQLNQDTIERYLLTTSVIGSLYKMNAS
                     ISGAEVGCQGEVGVACSMAAAGLTEIMGGSAEQVCMAAEIAMEHNLGLTCDPVGGQVQ
                     VPCIERNAIASVKAINASRMALRRTSSPQVSLDKVIETMYETGKDMNAKYRETSTGGL
                     AIKILPCD"
     gene            complement(2788..4377)
                     /gene="purH"
                     /locus_tag="PXB27_000472"
     CDS             complement(2788..4377)
                     /gene="purH"
                     /locus_tag="PXB27_000472"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005821463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDL5345299.1"
                     /translation="MAIQQALLSVSDKKGIVEFARGLSARGVKLLSTGGTAKLLAEAG
                     LLVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGTDDEVMNQHGIERIDMVVVNLY
                     PFAQTVANPNCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVNNNDFDAILAEMDQHQ
                     NSLTLETRFNLAIKAFEHTAQYDSMIANYFGQKVPPYQGAEEEDLTQPSGQFPRTLNL
                     NFVRKQTMRYGENSHQNAAFYVENELKEASVSTAKQLQGKALSYNNIADTDAALECVK
                     EFSEPACVIVKHANPCGVALGKNILDAYNRAYQTDPTSAFGGIIAFNRELDGETAQTI
                     ADRQFVEVIIAPTVSASAKEVLATKKNVRVLECGEFSEAVKRLDFKRVNGGLLVQDAD
                     QGSVSIDDLKVVSERKPTEAELKDLLFCWKVAKYVKSNAIVYAKDGQTIGIGAGQMSR
                     VYSAKIAGIKAEDEGLQVVGCVMASDAFFPFRDGIDAAAKVGITCVIHPGGSMRDQEV
                     IDAANEHGMAVVLTGMRHFRH"
     gene            4549..4818
                     /locus_tag="PXB27_000473"
     CDS             4549..4818
                     /locus_tag="PXB27_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL5345300.1"
                     /translation="MINHNLADFVFEYFTENNLKAGTLRYRYIKENVIDAYSTRVDDA
                     FQGKGIAGELYNALIAFAQEKGLKIKPSCSYIEVKMQRSHRDLIA"
     gene            complement(4905..6350)
                     /gene="dacB"
                     /locus_tag="PXB27_000474"
     CDS             complement(4905..6350)
                     /gene="dacB"
                     /locus_tag="PXB27_000474"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005615286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HDL5345301.1"
                     /translation="MIFKNFTRAFLNKFILSTLLIPSITQAEINTEELLNTLPAGTSV
                     SFIAKNLDTNQIITQYQSDIFMLPASTQKVFTALAAKLTLNDDFRFQTALLTNGKVEN
                     GVLKGNLIARFSGDPELTSGQIYQLMSKLKQQGINKIEGDLILDPSVFASHDKASGWI
                     WNDLTMCFNAPPAAINVDHNCFYVTLNADQPIGEFAKVNVPSAYPVQVFSSAYIVEPK
                     EAPFCQLDVVVHDNNRYQIKGCMARQSQPFGLSFSVQDPTNYGANMLKAQLKSLKIAF
                     NGQVKEPLTAQNGTLLAEHYSEPLPVLLKKMMKKSDNQIADALFRTVANKQHNRPASF
                     QLGSYVIRQLLKTKANIDFKNSVVADGSGLSRHNQVSSRTMLETLEYIAQNEESLKLF
                     ETFPIAGVDGTISGRGSISTEPLAKNLIAKTGSLKGVYNLAGFMKNARGERIAFVQFI
                     NGYSTGELESKTKRAPLNTFENKFYMALFNE"
     gene            6447..6923
                     /gene="greA"
                     /locus_tag="PXB27_000475"
     CDS             6447..6923
                     /gene="greA"
                     /locus_tag="PXB27_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDL5345302.1"
                     /translation="MKQIPMTVRGAEQLREELDFLKNVRRPQIIDAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIQEIEGKLGNAQIIDVTKMANNGKVIFGATVQLVNADTDEE
                     VTYRIVGDDEANIKEGLISVNSPIARGLIGKEVDDSVSITTPGGKVEFDIVGVEYI"
     gene            complement(6979..7395)
                     /gene="ndk"
                     /locus_tag="PXB27_000476"
     CDS             complement(6979..7395)
                     /gene="ndk"
                     /locus_tag="PXB27_000476"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDL5345303.1"
                     /translation="MIQQTLSIIKPDATRRNLIGKILAQLEENGLTIKALKKVHLTRE
                     QAEGFYAEHKGKDFFEPLVEFMTSGSIVVSVLEGENAISHYRALMGATNPDNREKGTI
                     RDLYALSMRENSVHGSDSEESAKREIAYFFVPSEIV"
     gene            complement(7405..9483)
                     /gene="metG"
                     /locus_tag="PXB27_000477"
     CDS             complement(7405..9483)
                     /gene="metG"
                     /locus_tag="PXB27_000477"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDL5345304.1"
                     /translation="MANQKRKMLVTCALPYANGAIHLGHMLEHIQADIWVRFQRMRGN
                     EVHFVCADDAHGTPIMLNADKLGITPEQLIEKAKADHEADFASFNISFDNYHSTHSEE
                     NRQITTDIYKTLKANGFIKSKVISQLFDPEKNMFLPDRFVKGTCPKCKAEDQYGDNCE
                     VCASTYSPMDLINPRSAVSGATPVVKESEHFFFDLPSFEGMLKEWTRSGSLQPEIANK
                     MQEWFESGLQQWDISRDAPYFGFEIPDAENKFFYVWLDAPIGYIASFKNLCDRAGIDF
                     DEFWQKDSTTELYHFIGKDIVYFHSLFWPAMLEGSGYRKPTNVFAHGYVTVDGAKMSK
                     SRGTFIQANTYLKHLDPECLRYYYAAKLNDRIEDLDFNLDDFVQRVNSDIVNKLVNLA
                     SRNASFIAKRFEGKLAEKLDDEALFNEFTAQAETIANFYENREFNKAIRAIMELTDKA
                     NKYIDDKAPWVIAKEEGKEAELQAVCSMGIELFRVLMSYLKPVLPKLAERAEAFLQSE
                     LRWDNIATPLFGHTVAPFKSLFSRLEKKQIDAVIDETKALFAEANKKTENQTKGTQKV
                     EQQAVEKQENIAVEPISPEITIDDFAKLDLRAAKVLNCEAVPKSDKMLRFELDLGDHQ
                     RQVFSGIKAAYPNPEALIGRFVIVIANLAPRKMSFGMSEGMILSAGTGGANLFLLDAD
                     SGVQPGMQVK"
     gene            9695..10666
                     /gene="menC"
                     /locus_tag="PXB27_000478"
     CDS             9695..10666
                     /gene="menC"
                     /locus_tag="PXB27_000478"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HDL5345305.1"
                     /translation="MRQVKLYRYQLPIQTGVVLRKQPLKERQGVLVCLQEQEKVGWGE
                     IAPLPSFSQESLEQAENQAVNWLKAWQKGEPETLEQLFPSVAFGLSCALAELNNELGE
                     QANNQSAVLCYGDLEKLHTAFSQHTNPIGKIKIGLNPEQEGEQANLLLHTFPNLRLRL
                     DANRLWSLEQAVKFAEKIANEHKPRIQFIEEPCQALELSRQFATQTDIAIAWDETVRE
                     ANFLVKKEPNLTAIIIKPTLVGSLEKCVNLIQQAQKQGLVAVISSSLESSLGLTQLAR
                     IAQQYTPNSVPGLDTLNLMQHQLLRDWHDSSLPLVGLESEFVREVVV"
     gene            10911..11612
                     /gene="rsuA"
                     /locus_tag="PXB27_000479"
     CDS             10911..11612
                     /gene="rsuA"
                     /locus_tag="PXB27_000479"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HDL5345306.1"
                     /translation="MRLDKFIAENTGLTRSQAAKVLKSGVVTVNGNIEKSGAAKISQE
                     DEIYYEDQKLEWVEEGQYFMLYKPQGYICSHDDGEYPTVFQFFDYPLMTKLHTAGRLD
                     VDTTGLVLLTDDGKWSHRITSPKHHCEKTYLVTLADPVEDFYAQRLAEGILLRGEKEP
                     TLPAQLEILDDYNVNLTISEGRYHQVKRMFAALGNKVEALHRWRIGDVVLDERLEEGE
                     FRSLTSEEIASFNGK"
     gene            11602..12783
                     /locus_tag="PXB27_000480"
     CDS             11602..12783
                     /locus_tag="PXB27_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HDL5345307.1"
                     /translation="MESRRPSLSFFIILGMMAMLPPLAIDMYLPSFLDIARDLAVSQE
                     KVQTTLAVFTLGFGAGQLFWGPMADSFGRKIIILIGLVGSAIAAYFLTQVQNIETFYL
                     LRLVQGLCAAAPAVVLGALVRDLFERNLFAKLMSVIMIISMLAPLLAPIVGGYIAKYF
                     HWHSIFYVLVAMGILCVVLISWKIPETLAPEKRLPLSFGKTLSNFAILLKHRPTLGYV
                     LIGGLTFAGIFCFLTSGSIVYIGLYGVSQENFGYFFGLNMIVMVTMTAINSKMVVKVG
                     SETMLRIALAMQLLAGIWLALVAIFDLGFWAMAIGIPLYIGMLSTIGSNASAAILEQF
                     PQMAGTANGVAGTARFGVASLVGAGLSHIAVTSAAPMLLAMALCVMLAGVVYYFLCYK
                     R"
     gene            complement(12923..14242)
                     /gene="rlmD"
                     /locus_tag="PXB27_000481"
     CDS             complement(12923..14242)
                     /gene="rlmD"
                     /locus_tag="PXB27_000481"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDL5345308.1"
                     /translation="MAIFYSEKSAKKPIKSPACQKATIQSLDYQGLGIAKINGKTWFI
                     ENALPDEQVEFKVLEEKRQYGRGQAVKILKKSANRRMPSCELYGQCGGCQMQHISLEL
                     QRKAKQQALFQRLQKLQTHAIDVQPMIIGNDKHYRRRAKLSIAIQQGKAVIGFRLQNS
                     NQIIPLAHCEVLVEPLANLLPKLKHLIENWQQPKKLGHIELVSADNTIALFFRHLGAL
                     SAQDNHNLRQFATQEQLSLFVMTSENHIEHLCGERPYYQIQGLKLHFSIRDFIQVNAT
                     LNEKMVEKALEWLELSNQDRVLDLFCGMGNFTLPIAERAKSVVGVEGVEPMVQQAREN
                     AVTSGLKNVEFYQTNLDEPFSDKPWAKEPFNKVLLDPARNGALFCLDHLTSLNPERIV
                     YVSCNPATLVRDAEKLLQLGYRLEKAAMIDMFPHTGHLESISLFLKS"
     gene            14327..15652
                     /locus_tag="PXB27_000482"
     CDS             14327..15652
                     /locus_tag="PXB27_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase
                     family protein"
                     /protein_id="HDL5345309.1"
                     /translation="MISSVWNERYLADKKREQDHRSPFQRDRARLLHSEAFRCLQAKT
                     QIHAVGEDDFYRTRLTHSLEVAQIGSSLREKLLADVEGFQAVCSEQEFAKNMANLTAL
                     LPDHSLVESLCFAHDLGHPPFGHGGEMALNYKMYAFGGFEGNAQSFRIMTQLEPYTET
                     AGMNLTRRTLLGVMKYPALLSQTSPIEPAEMSEARHINLHHFPQSKGIFDDNNHFFEW
                     VLAPLSEKDRRQFCTVQPNQDPKQPNKTQYKSLDCSIMELADDIAYGVHDLEDAIVGG
                     MVTPQSWQNAEKLLAECQSDWVKQRLPEIREKLFSQHRYERKDVIGALVNHFITNVRW
                     KALPEFDEPLLRYNAYLPESVACVLKILKDFVYQYVICDAKTQRVERKGQRILMELFD
                     ILSSDPMRLLPNNVRERWKNAEESKRARVICDYLAGMSDGQAFKLFESL"
     gene            15773..16072
                     /locus_tag="PXB27_000483"
     CDS             15773..16072
                     /locus_tag="PXB27_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345310.1"
                     /translation="MRFLPSEIYQPRGELVKADRQGNGEFEVEYRVSGNDVRGLAKSA
                     IAHAKRKGFHLVESDIHRDDADLKFKRGDQELDIEIEVKGRNRIEYKADLDLDKN"
     gene            16233..19145
                     /gene="rapA"
                     /locus_tag="PXB27_000484"
     CDS             16233..19145
                     /gene="rapA"
                     /locus_tag="PXB27_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HDL5345311.1"
                     /translation="MFLVGQRWLSESENNLGLGIVTSVDHRTVTINFPAAEEERVYAI
                     AVAPLTRVQFQAGDRINSIDGWQLEVSEVVENQGAVIYLGKRVDNGEEAVLPEMLLDH
                     KISFSKPQDRLFSAQIDRSDRFALRFNALQHQQAQYQSPLRGLRGIRASLIPHQLHIA
                     KEVGQRLAPRVLLADEVGLGKTIEAGMILQQQLFSGRAERVLVLVPESLQHQWLVEML
                     RRFNLKFSLFDEERCSDFDKQDENGDDVSENPFESEALVITSLNWLETSPQRANQVLA
                     ADWDLLIVDEAHHLEWSETESSIGYQLVERLSQQIPAVLLLTATPEQLGQESHFARLA
                     LLDSDRFYDYAEFVQEQAQYKPVADAVSSLLSDKPLSNDEQNAIAELLSEQDTEPMFR
                     AINSKKSDENDRLQARQELISELIDRHGTSRVLFRNTRQGVKGFPHREYHQISLEMPK
                     QYQNALKVMGMMGSVSQSDLLYPEYLFQRMNPNAKWAEFDPRIDWLITFLKNHRDEKV
                     LVICKQADTAIALEQVLREREAIRSAVFHEKMSIVERDKAAAYFAQQEEGAQVLISSS
                     IGSEGRNFQFAKDLVLFNLPDNPDLLEQSIGRLDRIGQKNDIQIYVPCFNLSAQQMLA
                     EWYHKGLNAFEETCPMGTAIFRELGEELQQFLQKPELSAQFDDFVAKTYKRQQYLKAE
                     LEKGQDRLLELNSNGGEAAQQLANDIAKEDNNPQLVNFALSLFDVIGVEQEDLGEQSI
                     VISPTGHMLVPDFPGLSEDGTTITFNRELALMREEVEFLTWDHPMIRNGIDLITSGDI
                     GKSAISLLVNNKLPAGTLLLEALYVVEAQAPKGLNLTRFLPPTPIRVLVDNKGNNIAP
                     QVSFAGLERQLKPVNKQMANKIAKMARADIEKLIKASEKAVEPQVQQVIETAKFEANS
                     KLKAELHRLESLKMVNKNIRADEVEALEQQLNASLAQLETANYRLDSLRVIVSNKA"
     gene            19272..19568
                     /locus_tag="PXB27_000485"
     CDS             19272..19568
                     /locus_tag="PXB27_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HDL5345312.1"
                     /translation="MYIDLQYAVPLLFEYDPNKSQINLAKYGIDFEQAKLLWEDERKV
                     VLEACIEPELRYFLIGKIYDKYWTAFFTPRNDVIRLISVRRSRKKEISYYEKYH"
     gene            19552..19794
                     /locus_tag="PXB27_000486"
     CDS             19552..19794
                     /locus_tag="PXB27_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HDL5345313.1"
                     /translation="MRNTISAEELERIFDEGKEDILQYCDLSTAHRPGQMKRVNVDFP
                     NWMVEALDKQARHLGVPRQALIKMWIAEKLPVQSPR"
     gene            19806..20474
                     /gene="rluA"
                     /locus_tag="PXB27_000487"
     CDS             19806..20474
                     /gene="rluA"
                     /locus_tag="PXB27_000487"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HDL5345314.1"
                     /translation="MALIEYNPPLEPFLDEVYRDNHILVINKPSGLLSVPGNRPEYYD
                     SAMTRVQQKYGFTEPAHRLDMATSGILLFALSKAAEKELKRQFREREPKKHYIALLWG
                     KLGEKVGDKGEMNFPLICDWENRPRQKICYERGKKAITHYEVLAHYPNNIARVKLTPI
                     TGRSHQLRVHSLALGHPIIGDKFYANPLAKSLSPRLCLHAESLTITHPITRELMTFTA
                     EPTF"
     gene            complement(20452..21027)
                     /gene="ampD"
                     /locus_tag="PXB27_000488"
     CDS             complement(20452..21027)
                     /gene="ampD"
                     /locus_tag="PXB27_000488"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HDL5345315.1"
                     /translation="MPNLKIEQGCLTGQKQVPSPHFSQRPDQEDISLLVIHYISLPPE
                     EFGGNFIDLFFQGKLEPTAHPYFEEIKDLRVSAHCLINREGEITQYVNFNDMAWHAGV
                     SCFEGREKCNEYSIGIELEGSNNQPFTQAQYQSLANLTKAIQQSYPQITDERITGHCH
                     IAPERKIDPGQYFDWAYYKHLLNEAKKSAQQ"
     gene            21087..21929
                     /locus_tag="PXB27_000489"
     CDS             21087..21929
                     /locus_tag="PXB27_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HDL5345316.1"
                     /translation="MLSYRHSFHAGNHADVLKHIVQLLILRALKEKEKGFLYLDTHSG
                     VGRYSLLSFESEKTGEYLEGVARLWERDDLPEEVAFYIDELKKLNRGGKLKYYAGSPL
                     LAVQQLREQDRAILTELHPNDFPLLRQEFAKAKNVQTKRENGFQQLKSALPPKERRGF
                     VLIDPPYELKEDYELVVKAIEEGYKRFATGVYAIWYPVVLRQHTKRIVRGLMDTGIRK
                     ILQIELAVRPDSDQRGMTASGMIVINPPWQLETQMKKILPYLTEVLVPEGTGSWKVEW
                     ITPE"
     gene            22052..22693
                     /gene="pyrE"
                     /locus_tag="PXB27_000490"
     CDS             22052..22693
                     /gene="pyrE"
                     /locus_tag="PXB27_000490"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDL5345317.1"
                     /translation="MQQYKHDFIEFALSRNVLKFGEFTLKSGRISPYFFNAGLFNTGR
                     DLAKLGEYYAKAIQASGIEYDVLFGPAYKGIPIATTVAVALANLFDVDKPCCFNRKEA
                     KDHGEGGNLIGSPLTGKILLVDDVITAGTAIRESMEIINANNAQLAGVLIALNRKEKG
                     KGELSAIQEVERDYGCQVFSIVDFDDLVAFIETSPQYAPYLPKMREYREKYGV"
     gene            22867..23956
                     /locus_tag="PXB27_000491"
                     /pseudo
     CDS             22867..23956
                     /locus_tag="PXB27_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607068.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
     gene            complement(24021..25211)
                     /gene="metC"
                     /locus_tag="PXB27_000492"
     CDS             complement(24021..25211)
                     /gene="metC"
                     /locus_tag="PXB27_000492"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HDL5345318.1"
                     /translation="MSNYKNLSTKLVHTGRKKRNTQGAVNPVIQRASSLVFETLEEKK
                     HATQNRYKGALFYGRRGTLTHFSLQEAMCELEGGAGCYLYPCGAAAVTNAILAFVEQG
                     NHVLMTGAAYEPTQEFCNALLKRMGVSTTYYDPMIGEGLKALVQPNTKVLFLESPSSL
                     TMEVPDIPTLVRVAREINPEIVIMIDNTWSAGVLFPALEYGIDISIQAGTKYLVGHSD
                     VMIGTAVSNARCWDKLREGSYLMGQMVDADSAYITARGLRTLGIRLKQHEQSSIAVAN
                     WLAQRPEVKAVYHPALESCPGHEFFKRDFLGSSGLFSFELCEKLSNDKLANFLEHFEL
                     FSMAYSWGGFESLILANQPEEIAKIRPAIERKLTGTLIRIHVGLEAVEDLIADLEQGF
                     NRIR"
     gene            complement(25270..25809)
                     /gene="dsbB"
                     /locus_tag="PXB27_000493"
     CDS             complement(25270..25809)
                     /gene="dsbB"
                     /locus_tag="PXB27_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HDL5345319.1"
                     /translation="MLYFFKNLSQSRSAWFLLMLSCLILEGTALYFQHGMGLVPCVMC
                     IYERVALFGILFAGILGFLGNRYAIIRYLAILIWLGSAFKGLVLSIKHHDYQVNPSPW
                     NQCEFKVNFPQTLPLDQWLPQVFAPGPVNCSQSQWEMFGFSMAQWLIFTFAVYLIVAV
                     IVLISQFKRSRQRNNFMFR"
     gene            complement(25872..27413)
                     /gene="nhaB"
                     /locus_tag="PXB27_000494"
     CDS             complement(25872..27413)
                     /gene="nhaB"
                     /locus_tag="PXB27_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton antiporter NhaB"
                     /protein_id="HDL5345320.1"
                     /translation="MSSIDALFKSFLGKSPEWYKLCILAFLIINPLIYFFINPFVAGW
                     FLVAEFIFTLSMALKCYPLQPGGLLAIEAVVIGMTNPHHIKEEIMANFDVILLLMFMV
                     AGIYFMKQLLLYIFTKLLVAIHSKKILSLSFCLSAAFLSAFLDALTVIAVIISVGTGF
                     YGVYHKVASGNNFEDSTDITNDEKIISNKEILEQFRGFLRSLLMHAGIGSALGGVMTM
                     VGEPQNLIIASQAEWGFIEFLLRALPVSLPVLICGIATCFLVEKYKLFGYGDRLPSKV
                     WVVLARYSQLKEQKMTKQDRLKMLVQAIAGIWLIIGLALHLADVGIIGLTIIIICTAF
                     CGITDEHTLGKAFQESMPFTALLIVFFSVVAVIIDLKLFEPIIAFVLSAEPHSQLSLF
                     YIFNGLLSMISDNVFVGTVYINEAKTALDAGIIDREQFDLIAVAINTGTNLPSVATPN
                     GQAAFLFLLTSSFAPLIKLSYGRMVYMALPYTIVLSIVGFLGLEFLLPSMTEVMINWG
                     WFITR"
BASE COUNT         7922 a         5424 c         6155 g         7930 t
ORIGIN      
        1 aatcttgttg tgttactgtt tcccatcgct aatatagcat agcttgttag ataattcttt
       61 aaaggagatt actatggcta tgccatctca acgtttttct cttacttggg tactaaattt
      121 atttggtact gcggtaggag ccggcgtttt atttttgcca attaatgcag gtatgggtgg
      181 gttctaccca cttattatta tgactttgtt agttggacca atgacatatt tagctcaccg
      241 tggcttaaca cgttttgtgc tttcttcaaa atacaaaggg agcgatatta cggctgttgt
      301 tcgtgaacat tttggtgaac aggccggtaa attgatcacg ctactgtact tctttgccat
      361 ttttccgatt ttactgattt atggtgtagg tattaccaat accgtgagtt cttttatgga
      421 gaatcagctt catatcgcgc caccatctag ggctgtatta tcgtttatgc taatagctgc
      481 tatgatcgga gtaatgttat taagtgagca agtcatgtta aaaatcacaa cttgtcttgt
      541 gtatccatta gtgttgattt tattgggctt atctatttat ttaattccac agtggaatac
      601 agctgcctta cagcaaatgc caactacagg agacttcctt actacacttt ggctgactat
      661 tcctgtttta gtctttgctt ttaaccattc accggctatt tcttcttttg cactttctca
      721 acagaaatat taccaagatg acaagaaggc tgaaattgaa tcagctaaag tattacgttc
      781 taccgccttt atcttggtgt tatttgtgat gttctttgta tttagctgtg tattaacttt
      841 gacacctgaa gaattagcac aagctaaagt gcaaaatatt tcgattcttt cttaccttgc
      901 gaataaattt gataatccga ttatttccta ttttggaccg ttggttgctt ttttggcgat
      961 tggtagctct ttctttgggc attatttagg tgcgcgcgag gggttagaag gattggttaa
     1021 tcaaatgcgt aaagagccga ttgatccaag caaattccgc aaaattaccg ctattacttt
     1081 tttgatcatt ttatggattg ttgcgactat caatccaagc attttaggct tcattgaaag
     1141 ccttggtggg ccgattattg cgatgatctt gtttattatg cctgtctatg ccgtatataa
     1201 agtaccggca cttgcacgtt ttaaaggtga atttggccat ttatttgtgc ttgttatggg
     1261 ttgtattgct atttcagcaa tagtatatgg gttactgtaa tctttctaaa atcaatgtag
     1321 aatacgccct tttattttct atgtgaattt gtgtttatga ttagtgtatt tgatatgttt
     1381 aaagtaggga ttgggccttc cagttcccat actgcggggc cgatgaaagc ggcaaaggaa
     1441 tttgtcgatg aattacaaaa tcgcaaattt ttaaacaaaa ttgaccgctt gcgggtagat
     1501 gtctatggtt cattatcact caccgggcga ggacataata cggatattgc catcataatg
     1561 ggattaatgg ggtatttgcc tcataatgtg gatattgaac gtattgagca gacgattgaa
     1621 aatctaaaac aaaataagca acttatcatt gctgaaagca gtgttgagcc taaagtgatt
     1681 gcgttcgatt tttatgagga tatgccattt catcgagatt tcttacctcg gcacgaaaat
     1741 ggtgtgaaat ttagtgcctt gagcggtgaa gagttgctat ttcaaaacac ctattattct
     1801 attggtggtg gctttattgt tgatgaagct aactttgagc aagttaaaaa ttcagatctt
     1861 tctgttccat tcccatatca gaatgcagca gatttacttg cacattgtaa gaataacggg
     1921 ttaccgcttt cttctttagt ctggaaaaat gagcttgctt tacgcagtag agtagaaatt
     1981 tcggattatt tggcaatgat ttggaataca atggaagcct gtattgagcg tggtttgaat
     2041 acggaagggt tactacccgg gcctctgaaa gtggtacgcc gtgccagtgg gctacgcaga
     2101 atgttagagg caaacagtaa attcaatacc gacccaatgc aaattatcga ttgggtgaat
     2161 atgtttgccc ttgccgtgaa tgaagaaaat gcggcaggtg ggcgagtagt caccgcacct
     2221 accaatggag cctgtggtat tgttccggct gtgttggcgt actaccaaaa atttattgca
     2281 cagcttaatc aagatactat tgagcgttat ttattgacta ccagtgtgat tggttcgtta
     2341 tacaaaatga atgcctctat ttccggtgct gaagtaggtt gccaagggga agttggtgtt
     2401 gcttgttcaa tggcggcagc aggtttaacg gaaattatgg gtggcagtgc tgaacaggtg
     2461 tgtatggctg cagaaattgc aatggagcac aatttaggcc taacttgtga ccctgttggc
     2521 ggacaagtgc aagtgccttg tattgaaaga aatgcaatcg cttcagtcaa agcgattaat
     2581 gccagcagaa tggcactacg ccgtacttct tcgcctcagg taagtttaga taaagtcatt
     2641 gaaacaatgt acgaaaccgg caaagatatg aatgcaaaat accgtgaaac ctcaaccggt
     2701 ggcttagcaa tcaaaatttt accttgtgat taattaatga aaaaggctac cttatcaaaa
     2761 taaggtagcc ttttagtttg tagaacatta atgacggaaa tgcctcattc cggttagtac
     2821 cactgccata ccgtgttcgt tggcggcatc aatcacttct tgatcacgca ttgaaccacc
     2881 tgggtgaatc acacaagtaa tccctacttt tgcagcagcg tcaataccat cacggaacgg
     2941 gaagaacgca tcggaagcca ttacacaacc cacaacttgt aagccctcgt cttccgcttt
     3001 aatacctgca atttttgcgg aataaacacg gctcatctgc cctgcaccga taccgatagt
     3061 ttgcccgtct ttggcataca caatcgcatt cgatttcaca tatttcgcca ctttccagca
     3121 gaacaatagg tcttttagct cagcttcagt tggtttacgc tctgaaacca cctttaaatc
     3181 gtcaatagat acagagcctt ggtcggcatc ttgcactaat aatccgccgt ttacccgttt
     3241 gaaatctaag cgttttacag cttctgagaa ctcgccacat tcaagcacac gcacgttttt
     3301 cttagtggct aacacttctt tggcagaagc agaaacggtt ggtgcaataa tcacttcaac
     3361 aaattgacgg tctgcgatgg tttgagcagt ttcgccgtct aattcacggt taaaggcaat
     3421 aatgccaccg aaagcagaag ttggatcggt ttggtaagca cggttgtagg catctaaaat
     3481 atttttaccc aaagcaacac cacaagggtt agcgtgtttt acaatcacac aagcaggctc
     3541 gctaaattct ttcacgcatt caagagccgc atcggtatct gcaatattat tatacgaaag
     3601 ggctttacct tgtaactgtt tggcggtgga aacagaggct tcttttaact cattttcgac
     3661 atagaatgca gcgttttgat gcgaattttc gccataacgc atcgtttgtt tacgcacaaa
     3721 attgaggttt aaggtgcgag ggaattgtcc acttggttgg gttaaatctt cttcctcagc
     3781 cccttgataa ggcggcactt tttgcccgaa atagttggca atcattgaat cgtattgtgc
     3841 ggtatgctca aacgctttaa tagcaagatt aaaacgggtt tctaaagtta aactgttttg
     3901 atgttgatcc atttcggcaa gaatagcatc aaaatcattg ttattgacca caatcgccac
     3961 atctttatga tttttcgctg cagaacgcac cattgtcgga ccaccgatat caatattttc
     4021 caccgcatct tcaaggctac aatttggatt ggcaacggtt tgagcaaaag ggtagaggtt
     4081 tactaccacc atatcaattc gttcaatgcc gtgttggttc atcacttcat catcagtacc
     4141 acggcgacct agaattccgc cgtgcacttt cgggtgcagg gtttttacac gcccgtccat
     4201 catttcagga aagccggtgt aatctgatac ttctgttact agcaaaccgg cttcagccag
     4261 taattttgcg gttccgcccg tagaaagtaa ttttacccca cgagccgata gcccacgagc
     4321 aaattcgaca atgccttttt tatcagacac acttaataac gcctgttgaa tcgccattct
     4381 taaattcctt aaaatgagtg ttaacgtgaa aataaacggc aaagattata acgcaaacgt
     4441 ttgctttttt catctacaag tggtcgattt tgcaaatttt tctgcaaaat cgaccgcttg
     4501 cactcgtgat taaagcgggt agaatagagg aaaaaaatcg gaggaactat gattaaccac
     4561 aatttagcag atttcgtatt tgagtatttt accgaaaaca acctaaaagc aggcacacta
     4621 cgttatcgct acataaaaga gaacgtgatt gatgcttaca gtacccgagt agatgatgcc
     4681 tttcaaggta aaggtattgc aggagagctt tacaatgctt taattgcgtt tgcgcaagaa
     4741 aaagggctga aaatcaagcc aagttgtagt tatattgagg taaaaatgca acgaagtcat
     4801 cgagatttaa ttgcttagag aggagcaagc ggtcgtaaaa tttcaaaaat ttacgaccgc
     4861 tgtcttattc aattctcaaa aatccatcac acaaaatata agccctattc attaaacaac
     4921 gccatataaa acttattttc aaatgtatta agcggagccc gtttggtttt gctttcgagc
     4981 tcgccggtgg aatagccgtt aataaattgc acaaaagcga tgcgttcgcc acgggcgttt
     5041 ttcataaagc cggcaaggtt gtaaacccct ttcagtgagc cggttttggc aatcagattt
     5101 ttggcaagcg gttctgttga aatagagcct ctgccggaaa tagtaccgtc cacgcctgca
     5161 atcgggaagg tttcaaacag ttttaagctt tcttcgtttt gagcgatata ttcaagggtc
     5221 tccagcattg ttcggctgct gacttgatta tgacgggaaa gccctgagcc atcagccacc
     5281 acgctgtttt taaaatcaat attggctttg gtttttaata actggcgaat cacataacta
     5341 cctaattgga acgatgcggg gcggttgtgt tgcttgttgg caacggttct aaacagggca
     5401 tcggcaattt ggttatccga ttttttcatc atttttttca ataataccgg caacggctcg
     5461 gaataatgct cggcaagtag cgtgccgttt tgggcagtaa gtggctcttt tacttgcccg
     5521 ttaaaggcaa ttttcaggct ttttaactgg gctttaagca tatttgcccc gtaattagtt
     5581 gggtcttgca ccgaaaaact taagccgaac ggttgagact ggcgagccat acagcctttg
     5641 atttggtagc ggttgttatc gtgaaccacc acatcaagct ggcaaaacgg agcttctttc
     5701 ggctcgacaa tataagcaga gctaaacacc tgcaccggat aagcggacgg cacattcact
     5761 ttagcaaatt cgccaatggg ttggtcggca tttaaggtga cataaaaaca gttgtgatcc
     5821 acattaattg ccgccggtgg ggcgttaaag cacatcgtca agtcgttcca aatccaaccg
     5881 gaggctttgt cgtggctggc gaaaaccgac gggtctagaa ttaaatcccc ctcaatttta
     5941 ttgatacctt gttgctttaa tttgctcatt aattgataaa tttgaccgct tgtaagctca
     6001 gggtcgccac taaaacgggc aattaaattg ccttttaaca ctccgttttc tacttttccg
     6061 ttggttaaaa gggcagtttg aaagcggaaa tcatcgttta aagttaattt tgctgccagt
     6121 gccgtaaaga ctttttgggt actggcaggt agcataaaaa tatcactttg gtattgggtg
     6181 atgatttggt tagtgtctaa atttttagca ataaaactaa cagaagtacc ggcaggcaag
     6241 gtatttaata attcttcggt attaatttca gcctgtgtaa tgcttggaat cagaagtgta
     6301 gataagataa atttatttaa aaaagcacga gtgaaatttt taaaaatcat aataaaaggg
     6361 gagttgaaat ttttagtgag aaattgaata atagcccttt cgttttaatt cgtaaaccga
     6421 taatttttag taaggaaata gatttaatga aacaaattcc tatgacggtg cgtggtgcag
     6481 agcaactgcg tgaagaattg gattttttaa aaaatgtgcg tcgcccacaa attattgatg
     6541 cgattgcaga agcccgtgaa cacggtgatt taaaagagaa cgcggagtat cacgcagccc
     6601 gtgaacaaca aggtttctgt gaggggcgta ttcaagaaat tgaaggcaaa ttaggcaatg
     6661 ctcaaattat tgacgtcact aaaatggcaa acaacggcaa agtgattttt ggggcaacgg
     6721 tgcagttagt gaatgctgat acagatgaag aagtcactta ccgcattgtg ggtgatgatg
     6781 aagccaatat taaagagggc ttaatttcag ttaattcccc gattgctcgt ggtttaatcg
     6841 gcaaagaggt ggacgattca gtcagcatta ccacaccggg tggcaaagtt gagtttgaca
     6901 ttgttggcgt agaatatatt taatctacgc tagggcgaat tacaatagtg gggcgaaaaa
     6961 attttcgccc ctactttatt aaacaatctc actcggtaca aagaagtagg caatttcacg
     7021 ttttgccgac tcttcggaat ctgaaccgtg taccgaattt tcacgcatac ttaaggcata
     7081 taaatcgcga atggtgcctt tttcacggtt atccggattg gttgccccca ttaatgcacg
     7141 ataatggcta attgcatttt ccccttccag tacagaaacg acaatagatc ctgatgtcat
     7201 aaattccact aacggctcaa agaaatcttt gcctttgtgt tccgcataaa atccttccgc
     7261 ttgctcacgg gtaagatgta ctttttttag agctttgata gtcagcccgt tttcttccaa
     7321 ttgagccaaa attttgccga ttaaattacg gcgtgtagca tccggcttaa taatagaaag
     7381 cgtttgttga atcatcatct tctcctattt aacttgcatt cccggctgaa caccgctgtc
     7441 tgcatctaat aagaacaggt tcgcaccgcc tgtgccggca gataaaatca ttccctccga
     7501 cataccgaac gacattttgc gtggagcaag gttggcaatt acaatcacaa aacgcccgat
     7561 aagtgcttcc ggatttgggt aggccgcctt aatgcctgag aatacttggc gttggtggtc
     7621 gcctaaatcc agctcaaaac gtaacatttt gtctgatttt ggcacagcct cgcagttcaa
     7681 tactttggct gcacgtaaat ctaatttcgc gaaatcatca atcgtaattt cagggctgat
     7741 tggctcaact gcaatatttt cttgtttttc gaccgcttgt tgttccactt tttgtgttcc
     7801 tttggtttgg ttttcggttt tcttattcgc ttcggcaaat aaggctttgg tttcatcaat
     7861 cactgcatca atctgttttt tctcaaggcg tgagaatagc gatttaaacg gtgccacggt
     7921 atgcccgaat aatggggtag cgatattatc ccaacgcaat tcgctttgta agaatgcttc
     7981 ggcacgttct gccagttttg gtaacaccgg ttttaggtag ctcatcagca cacggaataa
     8041 ctcaatgccc attgaacaaa ccgcttgtaa ttcagcctct ttgccttctt ctttggcgat
     8101 gacccaaggg gctttgtcgt caatgtattt attggcttta tcggttaatt ccataatggc
     8161 acgaatagct ttattaaatt cacggttttc atagaaattc gctatggttt ctgcttgagc
     8221 ggtaaattca ttaaataacg cttcgtcatc taatttttca gctaatttgc cctcaaagcg
     8281 tttagcgata aaactggcgt tgcgagaagc aagattcact aatttgttta cgatatcgct
     8341 gtttacacgt tgcacgaagt cgtctaaatt aaaatctaaa tcttcaatgc gatcgtttaa
     8401 ttttgccgca tagtaatagc gtaagcattc cggatcgaga tgttttaaat aggtgttggc
     8461 ttgaatgaat gtaccacgag atttcgacat tttcgccccg tccactgtca cataaccgtg
     8521 agcaaacaca ttggtcggtt tgcggtagcc gctgccttcc aacattgccg gccaaaacag
     8581 gctgtggaag taaacaatgt ctttaccgat gaagtgataa agctcggtgg tggaatcttt
     8641 ttgccaaaac tcatcaaaat caatgcctgc acggtcgcat aaatttttaa acgatgctat
     8701 ataaccaata ggggcatcta accaaacgta gaagaatttg ttttctgcgt ccggaatttc
     8761 aaaaccgaaa taaggggcat cacggctgat gtcccattgc tgtaaaccac tttcaaacca
     8821 ttcttgcatt ttattggcaa tttcaggttg gagtgagccg gaacgagtcc actctttcaa
     8881 cattccctca aagcttggca ggtcaaaaaa gaaatgttct gattctttta ccaccggagt
     8941 tgcaccggaa actgccgagc gagggttgat taaatccatc gggctatagg tagaggcaca
     9001 tacttcacag ttatcgccat attgatcttc ggctttacat ttcgggcagg tccctttcac
     9061 aaagcgatcc ggcaagaaca tatttttttc tgggtcgaac agttgtgaaa tcactttgct
     9121 tttaataaaa ccgttcgctt ttaaggtttt ataaatatcg gtagtgattt ggcggttctc
     9181 ttcgctgtgg gtggaatggt aattatcaaa gctgatatta aaactggcaa aatccgcttc
     9241 gtgatcggct ttggcttttt cgattaactg ctcaggggta atgcctaatt tatcggcatt
     9301 aagcataata ggcgtgccgt gggcatcatc agcacagaca aagtggactt cattgccacg
     9361 catacgttga aaacgcaccc aaatatctgc ttgaatatgt tcaagcatat gacctaaatg
     9421 aattgcaccg ttagcatagg gcaaggcaca agtaacaagc atttttcgtt tttggttagc
     9481 cattcttttt ctcattaaaa ttagggtaaa aattgccgtc tattttagcc caaaagtggc
     9541 aggtttacca gttggcgtga atttattaga gctttgggct aacaagcggt agaatatgat
     9601 gcgctagagg cgaaacattt ttcgcctcaa tttcccaaat gatgatgtgg gcgaaagatg
     9661 tttcgcctct aaacctattt tggataaaaa tagtatgcga caagtaaagt tataccgcta
     9721 ccaactcccc attcaaaccg gtgtagtgtt acgcaagcaa cctttgaaag aaaggcaagg
     9781 ggtgttggtg tgtttgcaag aacaggagaa agtcggctgg ggcgaaattg caccgcttcc
     9841 aagttttagc caagagagcc tagaacaagc tgaaaaccaa gctgtgaatt ggttaaaggc
     9901 gtggcagaaa ggagagcctg aaacgttaga gcagcttttc ccgtctgttg cctttgggct
     9961 aagctgtgcg ttggctgaac tgaataatga attaggcgag caagcgaata accaatcggc
    10021 ggtgctgtgc tatggcgatt tggaaaagtt acacacggca tttagccaac atacgaaccc
    10081 aatcggtaaa attaaaatcg gtctcaatcc tgaacaggaa ggcgagcagg caaatttgct
    10141 gttgcatact tttccgaatt tacgcctaag gcttgatgcc aaccgtcttt ggagcttgga
    10201 acaagcggtc aaatttgctg aaaaaattgc aaatgaacat aaaccccgta ttcaatttat
    10261 cgaagaacct tgccaagcgc tcgaactttc ccgccaattt gccacacaaa ccgacattgc
    10321 gattgcgtgg gacgaaaccg tgagagaggc gaattttttg gtaaaaaaag agccaaattt
    10381 gaccgctatt atcattaaac ctacgctggt tggttcgctt gaaaaatgcg tgaatttaat
    10441 tcaacaagca cagaagcaag gattagtagc ggtgattagt tcaagtcttg agagcagttt
    10501 gggcttaacc caactggctc gtatcgccca acaatacacc ccaaacagtg tgccggggtt
    10561 ggatacgtta aatttaatgc aacaccaatt attgcgagat tggcacgact ctagcttgcc
    10621 gttggtcggg ctagaaagcg aatttgttag ggaagttgtg gtataatctc ccaattaaac
    10681 actctctccc cttgtgggag agagaccgct tatgagcgtt cagcgaaata agcagagaga
    10741 ggggaagcgg tcatttttct aaaaaagttt gcacatttcc cctctccctg atttttcttc
    10801 tggacgaaga cgtatttccc ccctctccct gatttttctt ctagacgaag aaaaatctgt
    10861 ccctctccca cgaggggaga gggtaaagta atttatggta taaaaatagt atgcgtttag
    10921 acaaatttat cgccgaaaat accggtttga cccgttcaca agcggctaaa gtgctgaaaa
    10981 gtggtgtggt gacggtaaac ggcaatattg aaaaaagtgg tgcggcaaaa atcagtcaag
    11041 aagatgaaat ttattacgaa gatcagaaat tagagtgggt ggaagagggg cagtatttta
    11101 tgctctataa gccacagggc tatatttgtt cacacgatga tggtgagtac ccaacggtgt
    11161 ttcaattctt tgattatccg ttaatgacta aattgcatac cgcagggcgg ttagatgtgg
    11221 ataccacagg gctggtgctg ttaaccgatg acggcaaatg gtcgcaccgt attacctcgc
    11281 ccaagcacca ttgtgaaaag acatacttag tgacgcttgc tgatccggtg gaagattttt
    11341 atgcacagcg attggcagaa ggaattttat tgcgtggcga gaaagagcca accttgccgg
    11401 ctcaacttga aattttagac gattacaatg tgaatttaac tattagtgag gggcgttatc
    11461 atcaagttaa gcgaatgttt gccgcccttg ggaataaagt cgaggcattg caccgctggc
    11521 gaattggcga tgtggtgtta gatgaaaggt tggaagaagg tgaattccgc tcgctcactt
    11581 cagaggagat agccagtttt aatggaaagt agaagaccaa gcctgagctt tttcatcata
    11641 ttaggtatga tggcaatgtt gccaccatta gccattgata tgtatttgcc ctcattttta
    11701 gacattgccc gtgacttggc ggtttcgcaa gaaaaagtgc aaaccacctt ggcggtattt
    11761 accctaggtt ttggggcagg gcaactattc tggggaccga tggcagacag ctttgggcgg
    11821 aaaatcatta ttttaatcgg attagtcggc tcggcgattg ccgcttattt tctgacccaa
    11881 gttcagaata ttgaaacatt ttacctgcta aggttagtac agggcttatg tgcggcagcc
    11941 ccggcggtgg tgctgggagc attagtgaga gatctgtttg agcgtaattt atttgccaaa
    12001 ttgatgtcgg ttattatgat tatttctatg cttgccccat tgcttgcacc gattgttggc
    12061 ggttatattg ccaaatattt ccactggcac tcaattttct atgtgttagt ggcgatgggg
    12121 attttgtgcg tggtattaat tagttggaaa atccctgaaa cattagcccc cgaaaaacgc
    12181 ctgccgttaa gttttggcaa aactttaagc aattttgcca tcttactgaa acataggcct
    12241 actcttggtt atgtgctgat tggcggctta acctttgcag gtattttctg ttttttaacc
    12301 tcaggctcga ttgtctatat tgggctttat ggcgtgtcgc aagaaaattt cggctatttc
    12361 ttcgggctga atatgattgt gatggtgacg atgacggcga ttaacagcaa aatggtagtg
    12421 aaagtcggct ctgaaacgat gttacgaatt gctttagcta tgcaactgtt agccggcatt
    12481 tggctggctt tagtggcgat ttttgattta ggcttctggg caatggcaat cggtattccg
    12541 ctttatatcg gaatgctttc caccattggt agtaatgcca gtgccgccat tttagagcaa
    12601 tttccacaaa tggcaggcac ggctaacgga gtagcaggta cggctcgttt tggtgttgct
    12661 tcccttgttg gagcagggct ttcacatatt gctgtaacca gtgctgcccc aatgcttttg
    12721 gcgatggcat tgtgcgttat gttagccggt gtggtgtatt attttctgtg ttataagcga
    12781 taactaggtt ggggttagct tgcgaaaacc caacaaattt ctacaagcgg ttaataatag
    12841 tacatttttt gcaaattttt aggcagaatt aaccgcttgt gtggtacgag cgtggacgct
    12901 cgcaccatcg gggcaatatc tactaagatt ttaaaaacag cgaaatagat tccaaatgcc
    12961 cggtgtgggg gaacatatca atcattgccg ctttttctag cctgtagcct agttgcaaca
    13021 gtttttcggc atctcgcacc agtgttgccg gattgcaaga gacataaaca atccgctcag
    13081 gattgagtga tgtcaagtga tctaggcaaa atagtgctcc gttgcgtgcc gggtcgagta
    13141 gtaccttgtt aaacggctct tttgcccaag gtttgtcaga aaacggctca tctaaattgg
    13201 tttggtaaaa ttccacattt tttagaccgc ttgtcaccgc attctctcgg gcttgttgca
    13261 ccattggttc taccccttcc acaccgacca ccgatttcgc tcgctcagca ataggcaagg
    13321 taaaattccc cattccacag aagagatcta acactcgatc ttggttggat aattccagcc
    13381 actcaagggc tttttccacc attttttcat tgagcgtggc attcacctga ataaaatccc
    13441 gaattgaaaa atgcaacttt agaccctgaa tttgataata cgggcgttca ccgcacagat
    13501 gctcaatgtg attttcgcta gtcatcacaa aaagagataa ttgctcttgt gtggcgaatt
    13561 gacgcaaatt gtggttatct tgcgccgata atgcccctaa atggcggaaa aatagggcaa
    13621 tggtattgtc cgcactgacc agctcaatat gccccaattt tttcggctgt tgccagtttt
    13681 caatcaggtg ttttaatttt gggagcaaat tggcaagcgg ttcaaccagc acttcacagt
    13741 gtgcaagcgg tataatttgg ttggaatttt gcaaacgaaa gccgattacc gccttgcctt
    13801 gctggatcgc aatgcttaat tttgcccgac ggcggtagtg tttatcgttg ccgataatca
    13861 tcggctgaac atcaatagcg tgggtttgca atttctgtaa gcgttggaat aaagcctgtt
    13921 gttttgcctt acgttgcagt tcaagcgaaa tatgctgcat ttggcagcca ccgcattgcc
    13981 cgtaaagttc gcagcttggc attcgccgat ttgcagattt tttaagaatt ttaaccgctt
    14041 gtccacgtcc gtattggcgt ttctcttcca acactttaaa ttccacctgc tcatcaggca
    14101 gggcattttc gataaaccaa gttttgccgt tgattttggc aatccctaag ccttgataat
    14161 ctaacgattg aatagttgcc ttttgacaag cgggtgattt tatcggtttt tttgccgatt
    14221 tttcagaata aaaaatagcc attgttttca gatgaattgt tatgatataa agtgtcgaaa
    14281 tggggagatt atataagaaa agcagttggc ggaaaatgga ggcaatatga tttcatcggt
    14341 ttggaatgaa cgttatttgg cggataaaaa acgggaacaa gatcaccgct cgccttttca
    14401 gcgggatcgg gcaaggttat tgcattcgga ggcttttcgc tgtttgcagg cgaaaacgca
    14461 aattcacgcc gtgggggaag atgattttta tcgaacccgt ttaacccatt cgttggaagt
    14521 ggctcaaatc ggtagcagtt tgcgagaaaa attattagcg gatgtggaag gctttcaagc
    14581 ggtctgttcg gagcaggaat ttgcaaaaaa tatggcaaat ctgaccgctt tattgccgga
    14641 tcatagcttg gtggaatccc tttgttttgc ccacgatctc gggcaccctc cgttcgggca
    14701 cggtggtgaa atggcgttaa attataaaat gtacgccttt ggcggcttcg agggtaatgc
    14761 tcaatccttt cggattatga cccagcttga gccttacacc gaaacagccg gaatgaatct
    14821 gacccgtcgc accttactgg gggtaatgaa atatccggca ctcttgtctc aaacctcgcc
    14881 gattgagccg gcagaaatga gcgaggctcg gcatattaat ttgcaccatt ttccacagag
    14941 caaagggatt tttgatgaca acaaccactt ttttgagtgg gtattagccc cgttaagtga
    15001 aaaagaccga cgccaatttt gcacggttca gcccaatcaa gatccgaaac agccaaacaa
    15061 aacgcaatat aaatctctcg attgcagcat aatggaacta gcggacgata ttgcctatgg
    15121 cgtacacgat ttggaagatg cgattgtagg tggaatggtc acaccgcaat cgtggcaaaa
    15181 tgcggaaaaa ttgctggcgg agtgccaatc cgattgggtg aaacaacgcc tgccggagat
    15241 tcgggaaaag ctcttttctc agcaccgcta cgagcgaaaa gatgtgatcg gggcattagt
    15301 gaatcatttt attaccaatg tgcgttggaa agctctgcct gaatttgacg agccgttgct
    15361 acgttataat gcctatttac cggaaagtgt tgcctgtgtg ctgaaaatct tgaaagattt
    15421 tgtgtatcaa tatgtgattt gtgatgccaa aacccagcgg gtggaacgca aggggcaacg
    15481 cattctaatg gaattgttcg atattttaag cagtgatccg atgcgattac tccctaataa
    15541 tgtgcgggaa cgttggaaaa atgcggaaga atctaagcgt gcaagggtaa tttgcgacta
    15601 tttagccggt atgtctgatg ggcaggcgtt taaacttttt gaaagtttat aatgaacttt
    15661 gttaattttg caaggtctca gtaataggtt tcatttattt aataggagat ttcaatgaaa
    15721 actgtttcta aaggtctttt agtattagca actttggctt ctgcaacagc atttgcggtt
    15781 tcttccaagt gaaatttatc agcctcgtgg cgaattagtt aaagcagatc gtcaaggtaa
    15841 tggtgagttt gaagtagaat atcgtgtcag tggtaatgat gttcgtggct tagcgaaaag
    15901 tgcgatcgcc cacgctaaac gtaaaggttt ccatttagta gaatctgata ttcatagaga
    15961 tgatgctgat ttaaaattca aacgtggtga ccaagagctg gatattgaaa ttgaagtaaa
    16021 aggtagaaat cggattgaat ataaggcaga tctagattta gataaaaact aaatttaccc
    16081 cttaaaacga ccgcttgtgt gatttaaccg cacaagcggt ctttttattg tgcttttttg
    16141 caaaaatact caagccgacc ggtcccggaa tttggtaaaa tagccgttta aatgacaata
    16201 atgatgcttt tacttattac ttagaggaaa aaatgttttt agtcggtcag cgttggctaa
    16261 gtgaatcgga aaataattta ggtttgggaa tcgtgacttc ggtggatcat cgcactgtca
    16321 ctatcaattt cccggcagcg gaagaagagc gggtctatgc aattgcagtt gcacctttaa
    16381 ctcgagtgca gtttcaagcc ggagatagaa ttaacagtat agatggctgg cagttagagg
    16441 tttcagaggt tgtggaaaat caaggtgccg taatctattt gggcaaaagg gtggataacg
    16501 gtgaagaagc ggtactgcct gaaatgttgc tcgatcataa aatcagtttc agcaaaccgc
    16561 aagaccgcct tttttcggct cagattgatc gctcagatcg ctttgcatta cgctttaacg
    16621 ctttgcagca ccagcaagcg caatatcagt cgccactgcg tggtttgcga ggcattcgag
    16681 ccagcctgat tccacatcaa ttacatattg caaaagaagt cgggcaacgc cttgctcctc
    16741 gtgtgctatt agccgatgaa gtgggactgg gtaaaactat tgaagctggt atgattttgc
    16801 aacaacagct attttcgggc agagcagaac gagtattggt attagtgccg gaaagtttgc
    16861 aacaccaatg gttggtggaa atgcttcgcc gttttaatct gaaattctcg ttattcgatg
    16921 aagaacgttg tagtgatttt gataaacaag atgaaaatgg cgatgatgta agtgaaaatc
    16981 catttgagag cgaagcgtta gtcattactt cactaaactg gctggaaacc tcaccgcaac
    17041 gagccaatca agtgcttgct gccgactggg atctattaat tgttgatgaa gctcaccatt
    17101 tagaatggtc ggaaaccgaa tcttctatcg gctatcagtt ggtagagcgt ttatctcaac
    17161 aaattcctgc cgtcttgctt ttaacggcaa caccggagca attagggcaa gagagccatt
    17221 ttgcccgctt ggcgttgtta gattcggatc gtttttatga ttatgccgag tttgtacagg
    17281 agcaagcaca gtataaaccg gtggcagatg cggtgagtag cttattaagc gataaaccgc
    17341 taagtaatga cgagcagaat gcgattgcgg agctgttaag cgagcaagat accgagccga
    17401 tgttccgtgc aattaatagc aaaaaatcgg acgaaaatga ccgcttgcaa gcccgtcaag
    17461 agctgattag tgagctaatt gaccgccatg gaaccagccg ggtgttgttt agaaataccc
    17521 gtcagggagt gaaaggcttc ccacatcggg aataccacca aatcagccta gaaatgccga
    17581 aacaatacca aaatgcgtta aaagtgatgg gaatgatggg gagtgtgagc caaagtgatt
    17641 tgctctatcc tgaatatttg ttccaacgaa tgaacccgaa tgcgaaatgg gctgaattcg
    17701 accctcggat tgattggctc atcactttct taaaaaatca tcgtgatgaa aaagtgctgg
    17761 tgatctgcaa acaggcggac actgcgattg ctttggagca agttttgcgt gagcgtgagg
    17821 cgattcgttc tgccgttttc cacgagaaaa tgtcgattgt tgaacgtgac aaagctgccg
    17881 cctattttgc tcaacaagaa gaaggcgctc aggtgctgat tagttcaagc atcggttcag
    17941 aagggcgaaa tttccaattt gctaaagatt tagtgctgtt taatttgccg gataacccgg
    18001 atttattgga gcaaagtatt gggcgattag atcgtatcgg acagaaaaat gacattcaga
    18061 tttatgtgcc ttgctttaat ttatcggctc aacagatgtt ggcagagtgg tatcacaagg
    18121 gcttgaatgc gtttgaagaa acctgcccga tgggaacagc gatcttccgt gaattaggtg
    18181 aagaattgca acaattcctg caaaaacctg aactttcagc ccaatttgat gattttgttg
    18241 cgaaaaccta taaacgccaa cagtatttga aagccgaact ggagaaagga caggacagat
    18301 tattagaact gaattctaat ggcggagaag cagctcaaca gctcgctaat gatattgcga
    18361 aagaagataa taatccgcag ttggttaatt ttgccttgag tttgtttgat gtgattggcg
    18421 ttgaacagga agatttaggc gagcagagca ttgtgattag cccaaccgga catatgcttg
    18481 tgcctgattt cccggggcta tcggaagatg gcacgaccat tacctttaat cgtgaactgg
    18541 cgttaatgcg ggaagaggtg gaatttctca cttgggatca cccgatgatc cgcaatggta
    18601 ttgatttaat cacctccggc gatattggca aatcagcaat ctcattgctg gtgaataata
    18661 aattgcctgc cggtacatta ttacttgagg cgctttatgt ggtggaagca caagcaccga
    18721 aagggctaaa tttaacccgt ttcctaccgc caaccccgat tcgggtttta gtggataaca
    18781 aaggcaacaa tattgctccg caggtctctt ttgccgggct tgaacgccaa ttaaaaccgg
    18841 tgaataaaca gatggcaaat aaaattgcca aaatggctcg agcggatatt gaaaaattaa
    18901 ttaaagcgag tgaaaaagcg gtagaacctc aggtccaaca ggtgattgaa actgccaaat
    18961 ttgaggcaaa tagcaaatta aaagcagaat tacaccgctt ggaatcactc aaaatggtga
    19021 ataaaaatat tcgagccgat gaagttgaag ccttagaaca acagcttaac gcttcccttg
    19081 cccaacttga aacggcaaat tatcgcttag atagcttgcg agtgattgta agtaataaag
    19141 cgtaattttt tgcaagtacg aaaagtgatt ttttcgatct gtgtcgaaaa aatcactttt
    19201 ctattttgtg tcatccttgc agattattta tgctattcct catattgtaa aaggtataaa
    19261 aaaggaatat tatgtatata gatttgcaat atgctgtgcc gttattgttt gaatacgacc
    19321 cgaacaaaag ccaaattaat ttagccaaat atggtattga ttttgaacag gcaaagttgc
    19381 tttgggaaga tgaacgaaaa gttgtactgg aggcttgcat tgaacccgaa ttacgctatt
    19441 tcctgattgg caagatttat gataaatatt ggacagcgtt ttttacacca agaaatgatg
    19501 ttattcgttt aatttcagtt agacgttccc gtaaaaaaga gatctcatac tatgagaaat
    19561 accattagtg ccgaagaatt agaacgtatc ttcgatgaag gcaaagaaga tattttacaa
    19621 tactgcgatc tttctaccgc tcatcgcccg gggcaaatga aaagggtgaa tgttgatttc
    19681 ccaaattgga tggtagaagc gttagataag caagccagac atttaggtgt gcctcgccaa
    19741 gccttgatta aaatgtggat tgcggaaaaa ttaccggttc aatcacctag gtaagcgagt
    19801 ggcaaatggc attaattgaa tataaccctc cattagaacc atttttagat gaggtttatc
    19861 gggataacca tattttagta attaataagc ctagtggttt attatcggtg ccgggcaatc
    19921 gtcccgaata ttacgacagt gcaatgactc gagtacagca aaaatatggc tttaccgaac
    19981 cggctcatcg tttggatatg gcaacaagcg gtattctttt gttcgcccta agcaaagcgg
    20041 cggaaaaaga actaaaacgc caatttcggg aacgtgaacc gaaaaaacac tatattgcgt
    20101 tactttgggg caaattaggt gaaaaagttg gggataaggg cgaaatgaat tttccgctga
    20161 tttgcgattg ggaaaatcgc ccccgccaga aaatttgcta tgaacgaggc aaaaaagcca
    20221 ttacccatta tgaagtgtta gctcattatc cgaataatat agcgagagta aaactcacgc
    20281 ctatcacggg gcgttcgcat cagctacgag tacatagcct tgcgttaggt cacccgatta
    20341 ttggcgataa gttttatgct aatccgctgg caaaaagttt atcgccccgt ttatgtttgc
    20401 acgcggagtc actaacaatt actcacccga ttacaaggga gttgatgacg tttactgctg
    20461 agccgacttt ttagcctcgt tcagtaggtg tttgtaatat gcccaatcaa aatattgccc
    20521 cgggtcgatt ttccgttccg gggcaatatg gcaatgcccg gtgatgcgtt catcggtaat
    20581 ttgtggatag ctttgctgaa tggctttggt caaatttgcc aaagattggt attgagcttg
    20641 agtaaacggc tgattattac tgccttccag ttcaatgcca atcgaatatt cattgcattt
    20701 ctcccgccct tcaaaacaag agacgccggc gtgccacgcc atatcgttaa aattcacata
    20761 ttgcgtgatt tccccctccc tattaatcaa acaatgggcg gaaacccgta gatctttgat
    20821 ttcttcaaaa taagggtgag cggttggttc gagtttacct tggaaaaaca gatcgataaa
    20881 atttccacca aattcttccg gcggtaagct gatgtagtga ataaccaaca aggaaatatc
    20941 ctcttgatca ggacgttggc taaaatgcgg agacggcact tgtttttgac cggttaaaca
    21001 gccctgttcg atttttaaat ttggcatagt ctaaataggg tgaatacgtt aaaatatggc
    21061 tcattttact agaaaaagag aagaatatgt taagttatcg ccacagtttc cacgcaggca
    21121 accacgctga tgtgctaaaa cacattgtcc agcttttgat cttgcgagca ttaaaagaaa
    21181 aagaaaaagg ttttttatat cttgatactc actccggtgt ggggcgttat agtctgttaa
    21241 gttttgaatc ggaaaaaaca ggcgaatatt tagagggcgt tgcccgttta tgggagcgtg
    21301 atgatttacc ggaagaagtg gcattctata ttgatgagct gaaaaaatta aaccgtggcg
    21361 gcaagttaaa gtattacgca ggctccccat tattggcggt acagcaactg cgtgagcaag
    21421 accgagccat tcttaccgaa cttcacccca atgattttcc gctgttacga caagaatttg
    21481 ccaaagcgaa aaatgtgcaa accaagcgag aaaatggctt tcaacaatta aaatccgccc
    21541 tgccgcctaa agagcgtcga ggttttgtgt tgattgatcc accttatgaa ttaaaagaag
    21601 attatgaatt ggtggttaaa gcgatagaag agggctacaa acggtttgcc actggtgtgt
    21661 atgccatttg gtatccggtg gtgcttcgcc aacataccaa acgtatagtg cgaggtttaa
    21721 tggataccgg cattcgcaaa attctacaaa ttgagcttgc cgttcgtcct gatagcgacc
    21781 aacgtggaat gacggcaagt ggtatgattg tgatcaaccc accgtggcag cttgaaaccc
    21841 agatgaaaaa aatcctgcca tatttaaccg aggtgcttgt gccggaagga acaggttctt
    21901 ggaaggtgga gtggattacg cctgagtaaa ttaaatacgg atagcacaag cgtggacgct
    21961 tgcgccatca taaattctga tttgcaatat tttttagaaa aataaccgct tgtcttatca
    22021 agaagtgggg gaaatataaa aggaaaaaac gatgcaacaa tacaaacacg actttattga
    22081 atttgcctta agccgcaatg tgctgaaatt tggcgaattt accttaaaat caggcagaat
    22141 cagcccctat tttttcaatg ctgggctttt taacaccggg cgcgatttag ccaaattagg
    22201 cgaatattac gccaaagcca ttcaagccag cggcattgag tatgatgtgt tattcgggcc
    22261 ggcttataaa ggcattccga ttgcaaccac ggttgccgtg gcactagcca acctgttcga
    22321 tgtggataaa ccttgttgct ttaaccgcaa agaggcaaaa gatcacggtg aaggcggtaa
    22381 tttaatcggt tctcccttaa cgggcaagat tttgctggtg gacgatgtga ttaccgcagg
    22441 cacggcaatt cgtgagtcga tggaaattat caatgccaat aatgcccagc ttgccggtgt
    22501 gctgattgcg ttaaaccgta aagagaaagg caagggcgag ttgtctgcga ttcaagaagt
    22561 cgaacgtgat tacggttgcc aagttttctc gattgtggat tttgatgatt tggtagcatt
    22621 tattgaaacg tcgccacaat acgctcctta tttgccgaaa atgcgtgaat atcgtgaaaa
    22681 atacggtgtt taacttttgc aaaaaaacag caaaaactca ccgcttgtca ctctctaatc
    22741 tcaccattat gaatcacttt catcttttgg tgagattttt tcaattaaaa agccgataaa
    22801 ttgattgtga gctttagcta attggcttag gataaactcc tgtaatacag gagttggaga
    22861 actaaaatgg cacatcaaaa attacctttt cacgcagaca ccgttggctc atatttgcgt
    22921 tccgatgctt ggaaaaaagc tcacgcagac tataaagcag gcaacatttc cctcgaacaa
    22981 cgtgatgaaa ttgtagaagc ggaagtgaaa aaattagtac aagcatcaat tagatgcggg
    23041 cattcaagtt gttacagacg gcgaatatca ccgctcttgg tggcatattg atttcttgga
    23101 aaatctgaac ggcattgaag gctatgtgcc ggaaaaagcc tatgcgttta aaggggttga
    23161 ggttcgccct tataatacac gttgttgcgg taaagtgtct tggaatccga atcacccttt
    23221 tattgagcat ttcaaaaaat tcaatgcgat tgtaggtggt cgtgcggtgg cgaaatttac
    23281 cattccaagt ccaaaccaat taatgtaccc gattcagtgg gatcacggca tttacccaaa
    23341 ccgtgaggaa tttgccaaag atgtgcaaca ggcttataaa gaggcgatta aagcgttcta
    23401 tgatgccggc tgtcgttatt tgcaaattga tgatgtctat tgggggtcgt tgtgtaataa
    23461 cgttggtcaa ccgacatttg aagccgataa agctcaagcg gtggcgaata ttcaagccat
    23521 tcttgccgat aagccggaag atttaaccat taccacccac gtttgccgag gcaactataa
    23581 atcttcttat ttattgacag gggcttatga tccgattgcc gacggcttat ttgctcaaac
    23641 caactatgat gggtatttct tagaatatga tgacgagcgt tcaggcggtt ttgagccgtt
    23701 aaaacatttt gccaacaaca aagggcgtgt ggtattgggc ttaattagtt caaaattccc
    23761 tgaattggaa gataaggaag cggttaaagc ccgtattcaa gaagcggcaa aatatgtgcc
    23821 gttagagcag ctttgcctaa gcccgcaatg tggttttgcc tcaaccgaag agggcaatat
    23881 tatgtcggaa gcacagcaat gggcgaaagt tcgtcttgtg gaggaaatcg cccaagaagt
    23941 ttggggcgaa gattaattag agtgaaaaat gcccgtaaca agcgggcatt ttttgtgaat
    24001 tttttgcaaa ttcaagccat ttagcgaatg cggttaaaac cttgttctaa atcggcaatc
    24061 aaatcttcca ccgcttccaa gccaacgtga atgcgaatta atgttcccgt cagtttgcgt
    24121 tcaatcgccg ggcggatttt ggcaatttct tccggctgat tggcaagaat cagcgattca
    24181 aacccacccc aagaatacgc cattgaaaac agttcaaaat gctctaggaa gttggctaat
    24241 ttgtcattcg agagcttttc gcaaagttca aatgaaaata gaccgcttga acctaaaaaa
    24301 tcccgcttaa aaaattcgtg ccccgggcag ctttccaacg ccgggtgata aactgctttc
    24361 acttcagggc gttgagccag ccagttagct accgcaatgc tactttgttc gtgctgtttt
    24421 aagcgaatgc ctaaggtgcg tagcccccga gcggtgatgt aagcggaatc agcatccacc
    24481 atttgcccca ttaaataaga gccttcacgc agtttatccc aacaacgggc gttagaaacc
    24541 gcagtaccga tcatcacatc agaatgcccg actaaatatt tagtgccggc ttgaatggaa
    24601 atatcaatgc catattctaa agcagggaac agcacaccgg cagaccaagt gttgtcgatc
    24661 ataatcacga tttctgggtt gatttcacga gcaacacgca cgagagtcgg aatatcaggc
    24721 acttccattg ttagcgagct cggcgattct aaaaaaagca ctttggtgtt cggttgcaca
    24781 agggctttca agccttcacc aatcatcggg tcataataag tagttgaaac acccatacgt
    24841 tttaacagcg cattacagaa ttcttgtgtc ggttcgtaag ctgcgcctgt cattaaaacg
    24901 tggttgcctt gttccacaaa agctaaaatc gcattggtga ctgccgccgc accgcaaggg
    24961 taaaggtagc aacctgctcc accttctaat tcacacatcg cttcttgtaa tgaaaaatga
    25021 gtgagagtgc cacgtctgcc ataaaataat gctcctttgt agcggttttg tgtagcgtgt
    25081 tttttctctt ctaaggtttc aaacactaaa gatgaagcac gctgaatcac cgggttaact
    25141 gctccttgtg tgttacgttt tttacgccct gtatgaacaa gtttggtgga aagattttta
    25201 tagttagaca taacttttcc taaagtaaaa tgcatagggg caaatcaaca tttgcccctt
    25261 tattaataat taacgaaaca taaagttatt tctttgacgg ctacgtttaa attggctgat
    25321 tagcacaatg accgcaacaa ttaagtaaac cgcaaaagta aagattagcc attgtgccat
    25381 tgaaaagccg aacatttccc attggctttg gctacaatta accggaccgg gggcaaacac
    25441 ttgtggtagc cattggtcaa gcggcaaggt ttgcgggaaa ttaactttaa attcgcattg
    25501 attccatggt gatggattga cctgataatc gtggtgtttg attgaaagta ctagcccttt
    25561 aaatgcgcta ccaagccaaa ttagaattgc caagtagcga ataatcgcat atctattacc
    25621 aagaaatcct aaaataccgg caaataaaat accaaataaa gcaactcgct cataaataca
    25681 cattacacaa ggtactaatc ccatcccatg ttggaaataa agcgctgtgc cttcaagtat
    25741 aagacagctt aacattagta aaaaccatgc actgcgagat tgtgatagat ttttaaaaaa
    25801 atagagcata gttattcctc attttgtgaa cttaaggcat tctaccttat ttggtatgag
    25861 ttatctataa actaacgtgt aataaaccat ccccaattta tcattacttc agtcatagaa
    25921 ggtagtaaga attctaaccc taagaaaccg acaatagata gaacaatggt ataaggtaat
    25981 gccatataga ccattctgcc gtaagacagt ttaattaacg gcgcaaagga ggacgttaat
    26041 aagaataaga aagcagcttg tccgtttggg gtagcaacag aaggaagatt tgtgccagta
    26101 ttaattgcaa ctgcaatcag gtcgaactgc tcacgatcta taatgccggc atctagtgcg
    26161 gttttagctt cattaatgta aacagtaccg acgaagacgt tatctgaaat catcgagagc
    26221 aaaccattga aaatatagaa taatgaaagc tgtgaatgtg gttcggcaga taacacaaag
    26281 gcaatgatcg gctcaaataa ttttaaatca ataatcacgg caacaaccga gaaaaacaca
    26341 ataagcaatg cagtaaaagg catagattct tggaatgctt tccctaaagt gtgttcatca
    26401 gtaatgccac aaaatgcagt gcaaataatg atgatggtta agccaataat ccctacatcg
    26461 gcaagatgta gcgctaagcc aataattaac caaattccag caatggcttg caccagcatc
    26521 tttaagcgat cttgtttcgt cattttttgt tcttttagtt ggctatagcg agcgagtacg
    26581 acccaaactt tgctaggaag ccggtctcca taaccaaata atttatattt ttctacaaga
    26641 aagcaggtcg caatcccaca aattaacacg ggtaagctaa ctggtaatgc tctaagtaag
    26701 aattcgataa atccccattc cgcttgggag gcaataatta agttttgtgg ttctcccacc
    26761 atggtcatta cgccacctaa agctgagcca ataccggcgt gcatcaataa acttcttaaa
    26821 aaaccacgaa attgctctaa aatttcttta ttagagatga ttttttcatc attggtaata
    26881 tcagtagaat cttcaaagtt attacctgat gcgactttat gatatacgcc gtagaaacct
    26941 gttccaacgc taatgattac agcaataacc gttaaagcat caagaaaggc ggataaaaaa
    27001 gcagcgctta agcagaaaga aagggaaaga atttttttcg aatgaatggc gacaagcagt
    27061 ttggtgaaaa tataaagcaa taattgcttc ataaagtaaa taccggcaac cataaacatt
    27121 aaaagaagaa taacatcaaa gtttgccata atttcttctt taatgtggtg aggatttgtc
    27181 ataccgatta caactgcttc aatcgctaat aaaccacctg gctgtaatgg ataacatttt
    27241 aatgccatag agagagtaaa aataaattca gcgactagaa accagcctgc aacaaaagga
    27301 ttgataaaga aataaatgag aggattgata attaaaaatg ccaaaatgca caatttatac
    27361 cactcggggc tttttcctaa gaaacttttg aacagagcat caatgctact catataaagg
    27421 acctcaaaat t
//
LOCUS       DANLUQ010000014        26852 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.014, whole genome shotgun sequence.
ACCESSION   DANLUQ010000014 DANLUQ010000000
VERSION     DANLUQ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 26852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26852
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.014"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            114..761
                     /locus_tag="PXB27_000495"
     CDS             114..761
                     /locus_tag="PXB27_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HDL5345321.1"
                     /translation="MSIFQQTNPNRRRYGLAVFIGIIAGIISAFVKWGAEHPFPPRSP
                     IDLFTAACPQPVLDAMNAAADQVAAKTIALQECSRAFLNPPHVFLRDYIGIDPTEAAF
                     TFADHAFNWIGVTHMIFSLVFAIGYCIVAEIFPKIKFWQGIGAGIIANICVHYITFPA
                     LGLTPPVAEWPLYEHISELVGHIFWFWTIEVIRRDLRNRITREPDAEVPLDQPFR"
     gene            complement(894..2270)
                     /gene="trkA"
                     /locus_tag="PXB27_000496"
     CDS             complement(894..2270)
                     /gene="trkA"
                     /locus_tag="PXB27_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HDL5345322.1"
                     /translation="MKVIILGAGQVGSTLAENLVSEDNDIVLVDNDPTRLDKLKDKHD
                     LQVIRGEYASPQTLRDAGAGDADLLVAVTNSDEVNMIACQIAYTLFNVPTKIARIRSA
                     DYVRERERLFNDDVLPIDHIIAPEILVKEDILRLIHYPGALQITHFADELVSIVNVKA
                     YYGGPLVGYPISALRDHLPHIDARVVAIFRQDKAIVPQGSTIIEAGDEVFFICATQHI
                     RAVMSELQRLDRPHKRIMIVGGGSIGSSLARDLEDQYRVKIIERNPVRAEKLAEKLSK
                     TMVLTGDASDEELLFEEHIENIDLFLAVTSDDEANIMSALLAKRLGAKKVIILVQRPA
                     YLHLIQGGTIDIAISPQQATISALASYVRKGDILQVSSLKLGVAGAVEIIAHGDATTS
                     KVVGRQIRELKLPQGAIVGAVVRGEEVIIAHKSTMIAENDRVIIFVNDKKQIDEIEKL
                     FQLGVFFL"
     gene            complement(2270..3601)
                     /gene="rsmB"
                     /locus_tag="PXB27_000497"
     CDS             complement(2270..3601)
                     /gene="rsmB"
                     /locus_tag="PXB27_000497"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015431887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDL5345323.1"
                     /translation="MKKQSFKALSSRAISATIILQVLDQGKSLSTLIPDAQKQLEPKD
                     LPLVQEITFGVCRVLPRLESIIKLLVEKPLKGKTRLVHCLLLVGLYQLLYMRVPAHAA
                     VDEVVNATKTLKLDSFRALTNGVLRRFLREQEEILAKVDKHWQTLHPEWLVNKLKKAY
                     PNWREIVEANNQRPPMWIRVNQQHIKTNDYAALISELVEPEFAKNSENPTACVPKCAL
                     LLERAVNVNQLPHFEQGWATVQDAHAQWSAELLGAENGETVLDACAAPGGKTTHILEK
                     APKAKVMALDIEESRLSRVRENLVRLGQIAQVICGDASKPEEWLEEGVLFDRILLDAP
                     CSATGVIRRHPDIKWLRKEGDIAELATLQGEILKALWQRLKPNGILLYATCSVLPQEN
                     SEQIQRFLQDTPNAKQLEIDFNGEKVLEKQFFPQPNGGDGFFYAKLQKVAE"
     gene            complement(3653..4078)
                     /locus_tag="PXB27_000498"
     CDS             complement(3653..4078)
                     /locus_tag="PXB27_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL5345324.1"
                     /translation="MYNKLLIAIDLADLKSAKYVVDTALQMTAKNPNAVYRVVSIIEP
                     VDNSLISAFLPKNFDKEVVAEANQKLHEFTKNHFPEGSKVQHIVAYGTIYEEICRIAD
                     EKSVDLIMMLASSKPNAKGLSSNTVKVARNTTKPILVLR"
     gene            complement(4171..6819)
                     /locus_tag="PXB27_000499"
     CDS             complement(4171..6819)
                     /locus_tag="PXB27_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HDL5345325.1"
                     /translation="MSVESKVDYDDLQDMVASNDSGGRTPSGLAKKAIVYVAILWSVF
                     QIYYASPLPFVFQEWLTHAGINLNVVVDDTKARSIHLAFAMFLAFLSYPAFASSPKHH
                     IPKIDWVFAIVGAGLALYYIFFYEGLVKRFGAPNTQDIIAGCLGILLVLEATRRSLGL
                     PLVIIAIVFLIYNFFGQYFPADWIVSHRSGSLSQIINQQWITTEGVFGVALGVSTKYV
                     FLFVLFGALLDKAGAGNYFIKTAFAYLGHLRGGPAKAAVVSSALTGLISGSSIANVVT
                     TGTFTIPMMKRVGFTKEKAGAVEVASSVNGQIMPPVMGAAAFLMIEYVNMPYSQLITH
                     AFLPALISYIALVYIVHLEACKMNLQGLPRAEEPSPILVFLLRTVASILVIVGLAIGV
                     YYGLGWIRDVAEDYAFMIVCAFLGLWYLLAIRRVASYPDLEADDPNSEIVSLPKRKPT
                     VNAGLHYLIPVVVLLWCLMVEMLSPGLSAFWGTITLIFIQLTQRPLLNFFRKESVTKE
                     IIKQGVRDVVDSLEAGGRNMTGIALATATAGIIVGVVALTGFGVQLSSVIELLSMGNI
                     VLMLILVAVFSLILGMGLPTTANYIVVSSLMATVIMEVGRQNGLIVPLIAVHLFVFYF
                     GIMADVTPPVGLASFAAAAISGGSPINTGMVAFKYSLRTVILPFLFIFNTDLLLIDLR
                     YPGHGWVVFITATVGVLAFTSATMQYIVTKNKWWETALLVVAAFALFRPGFFMDRIYP
                     SERHIEPVQFEEQLLASKVGQNVTLKVEGLNPYGKEIEFYAQLPVPEGATGEERLKNL
                     GLTLIQTDEKIKVDGVETPKVVIDMVEMDSPAAKAGLNWDQTIHYIAVPQDAPSKDWI
                     FIPALLLVFGIVANQRRRVKKQCVIA"
     gene            complement(7021..7983)
                     /locus_tag="PXB27_000500"
     CDS             complement(7021..7983)
                     /locus_tag="PXB27_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HDL5345326.1"
                     /translation="MKKLVKLSFLASLVMGTASVQAAETFVTIGTGGQTGVYYVVGQS
                     ICQLVNRDSAKTGLKCNAPSTGASVANLNAIAAKEMDMGIAQSDWQYHAYNGSSSFEG
                     KKNDKIRAIFSLHPEPFTVMARTDSGIKSFDDLKAKRVNVGDPGSGTRATMNVILAAK
                     GWTDKDFKVASELKPAEMASVMCDNNLDAITYNVGHPNGALKEAAASCDAKLVPVTGP
                     EIDKLVADHSYYAKATIPGGLYKGSDEPTETFGVYATLVTSADVDADKVYNVTKAVFD
                     NFDRFKRLHPAFEHLKQEEMIKNALSAPLHEGAIRYYKEKGWIK"
     gene            complement(8167..10014)
                     /gene="selB"
                     /locus_tag="PXB27_000501"
     CDS             complement(8167..10014)
                     /gene="selB"
                     /locus_tag="PXB27_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HDL5345327.1"
                     /translation="MIICTAGHVDHGKTSLLQALTGTNTAHLPEEQKRGLTIDLGYAY
                     LPIENDILGFIDVPGHQRFLSNMLAGLGGIQHALLVISAEEGIKPQTEEHLDILHLLN
                     FQHIMIVITKADRAEETQISHLIEQVKTSYPFLAEAKTFVTSAKTQQGIEELKAHLIQ
                     LNQQHTQTHKPFRYAIDRVFNVKGAGLVVTGTTVAGKVSVGSELFLSNGKKVRVKAIH
                     AQNTPSETGVAGQRLALNIANVEKEEIKRGDWITELEPKFATDRITVQLTANQAFKEN
                     SIVHLYHFASHITGKLNLLEGQQAVKNQQFFAEVILDEPLHIAVGDKLIIRSGDDSLT
                     LAGAEVLEIHSPKRHKRTEARLALVKNLAKTTACNDFATRLDLYLQNRAMDLSLLLWA
                     EQCFAEDIAKLGFDVSSKWLFNSDFKAQAQQRVLEKMAEYHLQHQDQIGVTKARLYRI
                     ALLDLPENLANQFIEDLVEQKALVNSRGWLHLPEHRIELNADELQIWQQIRPLFEATN
                     QALWVRDIASQLSIDETQMRNLLYKVGKLGYLIPIVKDRFLLSEQIGQFATLIKDFIA
                     QNGSISVNQLRDELQYGRKLTVQLIEYFDRSGFLRRKGDVHLLRDSETF"
     gene            complement(10024..10320)
                     /locus_tag="PXB27_000502"
     CDS             complement(10024..10320)
                     /locus_tag="PXB27_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5345328.1"
                     /translation="MMKFDYNIVWLQKAEIKLFYQADYILQQSQNPIIAEQFYDAIKS
                     EVDKLSFTADVYRLRKKKEIPILNGKYQVKFLIGQENVYIVDFKSARQNSYSFL"
     gene            complement(10317..10493)
                     /locus_tag="PXB27_000503"
     CDS             complement(10317..10493)
                     /locus_tag="PXB27_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345329.1"
                     /translation="MELILAQDLAKLRTDKPQSFYTNDELDLLHQLENGLIETKSHAQ
                     VMEDLRKKLGITEK"
     gene            complement(10562..11194)
                     /gene="pdxH"
                     /locus_tag="PXB27_000504"
     CDS             complement(10562..11194)
                     /gene="pdxH"
                     /locus_tag="PXB27_000504"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005575912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HDL5345330.1"
                     /translation="MDLHHIREDYTKKVLSIRDCHSNPITQFEQWLSEAINAKVNEPT
                     AMNIATVAANGRPSSRMVLLKEVNSQGFVFFTNYQSRKGWEIADNPFGCLTFFWAELQ
                     RQVRIEGKIAKIPEAESDAYFDSRPYTSRLGAWASEQSQPIENYKALLLKSAQFALKY
                     PLSVPRPPHWGGYILIPDSIELWQGRSSRLHDRIHYWLENNEWKKERLSP"
     gene            complement(11252..12634)
                     /locus_tag="PXB27_000505"
     CDS             complement(11252..12634)
                     /locus_tag="PXB27_000505"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HDL5345331.1"
                     /translation="MQQLFRQIPSLDLLLKKPQAVELCQKFAYSSVAEQARSLLTQAR
                     QFISQQQALPDFIADENLFFIRLEQKVQALSEVKIRSVFNLTGTVLHTNLGRGLWSEK
                     AIQAATNAMRNNVALEFDIEAGKRSHRDNYISELLQILTGAEAACIVNNNAAAVLLML
                     ATFAQGKEVIVSRGELVEIGGAFRIPDIMAQAGCKLVEVGTTNRTHLKDYRNAITENT
                     AFLMKVHTSNYHIEGFTSSVSEEELVALGKEFNLPVITDLGSGSLTDMQALNLPAEPK
                     VQQKVAAGVDLVSFSGDKLLGGPQAGIIVGSQAMISQLQQHPLKRVLRCDKVILSALE
                     ATLRHYLFPEQLTDNLPTLHLLTQPLETLQHKAERLKQALHKRLNSAYILQIELSLAQ
                     IGSGALPTETLPSVAVTISAEKQSDLIALEQQFKTYPSPIIGRFAQQKFWLDLRSVAE
                     FDKLIEMLEY"
     gene            12702..12796
                     /locus_tag="PXB27_000506"
     tRNA            12702..12796
                     /locus_tag="PXB27_000506"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12736..12738,aa:Sec,seq:tca)
     gene            13003..13692
                     /locus_tag="PXB27_000507"
     CDS             13003..13692
                     /locus_tag="PXB27_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDL5345332.1"
                     /translation="MSTLSRDSILSQIQNGLIASCQPVDDGPMDKPEIVAAMAAASVI
                     GGAKGIRIEGVDNLKAARAAVNVPIIGIVKRDLPDSPVRITPFLEDIDALAEAGADII
                     AVDGTFRTRPVSIEEAVKRIHEKGCLAMADCSTLEEGLHCQQLGFDIVGSTMSGYTGG
                     EMPDEPDYQLVKDLKAAGCNVMAEGRYNTPELAKTAIEIGADYVTVGSALTRLEHIVS
                     WFADSVKSAQK"
     gene            13702..14604
                     /locus_tag="PXB27_000508"
     CDS             13702..14604
                     /locus_tag="PXB27_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HDL5345333.1"
                     /translation="MRCLAIDIGGTKIATAIVQHNQVSQRQQIQTPTDKPQADQAGSL
                     HQAIGEIMQHYQGQFDFVAVASTGIINKGVLTALNPKNLGGLAEFPLKQSIEKHTDKP
                     IALLNDVQAAVCAEYLNEDQQAVKNFVFITVSTGVGGGIILNGELQIGSNGIAGHIGH
                     TLADPNGPLCGCGRVGCVEAVAAGRAIEAVSSQWEKPCSPKEVFERFRQGNPQAVELV
                     QKSAKAIANLIADLKISLDIQKVVLGGSVGLAEGYLPQVSNYLAEMPSVYHCVLENAL
                     SGQDAGLIGAAWWAENQLNQGFTL"
     gene            14629..15507
                     /gene="nanA"
                     /locus_tag="PXB27_000509"
     CDS             14629..15507
                     /gene="nanA"
                     /locus_tag="PXB27_000509"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005718701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDL5345334.1"
                     /translation="MKNLKGIFSALLVSFNEDGTINEKGLRQIIRHNIDKMKVDGLYV
                     GGSTGENFMLSTEEKKEIFRIAKDEAKDEIALIAQVGSVNLKEAVELGKYATELGYDS
                     LSAVTPFYYKFSFPEIKNFYETIIRETGNKMIVYSIPFLTGVNIGVSQFAELFENEKI
                     IGVKFTAGDFYLLERLRKAFPNHLIYAGFDEMMLPATVLGVDGAIGSTFNVNGIRARQ
                     IFELAQQGKIKEAFEIQNVTNDLIEGILGNGLYQTIKGLLELEGVQAGYCREPMTKEQ
                     TPAQKEVTKQLKAKFL"
     gene            complement(15568..15774)
                     /locus_tag="PXB27_000510"
     CDS             complement(15568..15774)
                     /locus_tag="PXB27_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345335.1"
                     /translation="MDNKQTNGFNDIIVKADITQNIWSTGFYDIGRELKSENTRQEKV
                     RLKYNGDTLHRGRKSRMASLIASR"
     gene            complement(15732..16370)
                     /locus_tag="PXB27_000511"
     CDS             complement(15732..16370)
                     /locus_tag="PXB27_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345336.1"
                     /translation="MKKILLLVLLLTLATQADEGNNTQVCAEQQHQIIAHYFKISQEK
                     LSARTLASACKTAPNNSTETLVSYAVIEPKYSKENYQNDENTPKWTLFVARVNDNAIL
                     QSWEQDMEIDASIDVNADSLKIDTARYILNDKVRAFGVRFNNAARGPSAPEYSANDHL
                     MLFVPEKEKLKLVFDYPMTFWSILDDKETKTETQTWYCKWITNRPTASMILL"
     gene            complement(16612..17331)
                     /locus_tag="PXB27_000512"
     CDS             complement(16612..17331)
                     /locus_tag="PXB27_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL5345337.1"
                     /translation="MSKPNIYDESFSERMKWIAKNSFKDNYSEFARAVGVAQASLSRW
                     VKGEADPSRSNLVKIAEVSNVSLEWLATGKESKPQSENTSIVAREFERLEKMADDIVS
                     MINSFRTINVSAGFGSFNEGVTKPDGQEPYADSLLQSLGVKAHNCGVFWANGTSMHPT
                     IADSDQMLVDFSKREPRGDDKIYLVQNGESVWVKRIRKEWDYIELISDNESYRPIQIT
                     AEEAQNLQIIGQVVHIGHSLV"
     gene            17508..17786
                     /locus_tag="PXB27_000513"
     CDS             17508..17786
                     /locus_tag="PXB27_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5345338.1"
                     /translation="MSVLNDVKKTAQRDWHKADILAELRKKGWSLRSLAKAGNVSHNT
                     LKSVLDKPYPKMERLVANAIGVAPEVIWAGRTAERIERNRRPTLTNKF"
     gene            17831..18634
                     /locus_tag="PXB27_000514"
     CDS             17831..18634
                     /locus_tag="PXB27_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL5345339.1"
                     /translation="MSELSLKTHYSVAELLSFKLSSLPSAHKNVLEKAIRENWQSQKR
                     KGRGGGVEYELISLPTEVQQEIRTKLLKLLPAEISKGELSVVRQNIDLEQITDKQLST
                     ADARIMVVRWFLMQEVQLGLSRTKTLDQVIAAVASSEIPAEICKAIMAGNSKAGGKLK
                     LSKRTLHSWVLAYEAGENSAERLKQMIPLKTQKRAVPERCGWLQAFLPFYQTFSNVAL
                     TQAYAQFAMQYEGEDLPTESQVRYALKQLPDYVVQQGRKLPVKKIRLAR"
     gene            18601..19113
                     /locus_tag="PXB27_000515"
                     /pseudo
     CDS             18601..19113
                     /locus_tag="PXB27_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251293.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            19124..19498
                     /locus_tag="PXB27_000516"
     CDS             19124..19498
                     /locus_tag="PXB27_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5345340.1"
                     /translation="MSIVYEGSCVLEVDGVEIEITKLDVKTVTGRKLVKTMNSSGRAR
                     GYSQGIAEYTLSVTAVEPQDGSVIDWDNIKNAKLTKYPLDNSEKRTSYLGCFTTDVSE
                     SYTVDGESVRDIQLNALRKVNE"
     gene            19498..19866
                     /locus_tag="PXB27_000517"
     CDS             19498..19866
                     /locus_tag="PXB27_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345341.1"
                     /translation="MKITLNLGIEYNGKIRRDCTVRLLTMGGQCTAQEMIADLGFEEN
                     SEALSKREQTLVDMAYLSQQVTFDGIYQGNVTAQFLFDNLATEDYWQLIEATLTLRKK
                     RLENGVSQSESESPANPDLA"
     gene            19896..20084
                     /locus_tag="PXB27_000518"
     CDS             19896..20084
                     /locus_tag="PXB27_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345342.1"
                     /translation="MILLSKFAITAEQVWQMPLLEVFAWIESYLESEGVKTPHSPMGA
                     NSEVKRESYVFTRCGKIE"
     gene            20137..21132
                     /locus_tag="PXB27_000519"
     CDS             20137..21132
                     /locus_tag="PXB27_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL5345343.1"
                     /translation="MAKNMKVQLELTAKDTASPVLNQAAKTAEKAFKNTERAAQKSGN
                     AQTQAAQKAANATESSNRKIEQSYRNARRVAADVSRARETLGVRAENAIQREIEQTRA
                     AYDRLKRSGVASQNELRRASEATKQRVRELNAELGKTSFGDKAKGFGRGLMSAGVGIA
                     AGAAMARQPAKNQMEFDRQLAMASNTAFSDRDVKGRIEGKKELLAAVQEAVKVGGGTK
                     EDALLTLDTLIASGTMKAETAMSLLPTLQKGAVATGASSEDMAKIAISSMQQFGIKEE
                     DIGRALDMAVAAGQAGNFELADMARWLPQQMAAGNTARHWAVYYRLYRMTQTADG"
     gene            21081..21638
                     /locus_tag="PXB27_000520"
     CDS             21081..21638
                     /locus_tag="PXB27_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HDL5345344.1"
                     /translation="MGGLLPPVSYDPNGRWLALSLEVEGRELDRIDARAKTLIDAVDV
                     STGIFIDDWERVCGLTANSSLPLETRIERVIAKLNQLGGLSIGYITQVAKELGYCIKI
                     SEPQPFIAGLSRAGDMLWHKDIMWTFFVDVCNPTDDYKRFRASVSMAGDALMGRYKDP
                     ILESLLEEVKPAFSRVWVRYLGDIE"
     gene            21635..23695
                     /locus_tag="PXB27_000521"
     CDS             21635..23695
                     /locus_tag="PXB27_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015483985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5345345.1"
                     /translation="MKQVMNPINTPTQRFKDGNPATGEYGTIVTAEFLNNVQDSVINT
                     QQELHSVLAEAGIEANDEQVNQVAKAIKKIAGDATRDNFNALANPDGYKHIGRCKSVA
                     ELRTIRPTEHGQRILVDAYYEDGTTGGGEFVADLQDLVTPDDGGVCFVVNNNGGRWKR
                     VDLSHLTLFDFGAVGDGVTNDESAFVNAMRYSQFFIENGTFRINNAVNSVRDNVQILG
                     NKTGKLVLGAGIQQAEAEVFNINHSNYFISGFCIETPNKAIGIRFKSLDDAGVKNLHI
                     DNVVFNGTFYGVRAGESIQADTNYPTDNVIVQNCQSYCGSGNAGHYLCTKVKGVRFFN
                     NIAIGGRNVSAYGATSCSDIFILGNREQGMAVTTVDVEAGVQVEDVQTGECVVANNIC
                     SHDIWMSGAKNAIINSNLCRRLRVTSGNPTDTGSNNVEFSNNQAKSIAISKYGSFDNP
                     SLFIDNINFINNILEPSEFNQDVYIDGTILKQCQFINNRCISKSKQYSVRLARNNPCL
                     NLTFKSNKLSDNPAIISGKQGVIFCDDLSLGVNKHRLRLRFSSPINFTVNQEWQKMPF
                     DRVLNDINGHVDIVSGQFIARTGGTFHLKGKITVTNSSEDVLNLYQLRLLINNEEKER
                     LINQSVSPKNSKSNTNGMTTINFDTVVYLKVGDIVDLQYKTTHELTLLSDSFLSQIYV
                     TQEF"
     gene            23925..24135
                     /locus_tag="PXB27_000522"
                     /pseudo
     CDS             23925..24135
                     /locus_tag="PXB27_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484516.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(24318..25913)
                     /locus_tag="PXB27_000523"
     CDS             complement(24318..25913)
                     /locus_tag="PXB27_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5345346.1"
                     /translation="MRLSQLSLSVLLLSASSLVAAAPVNTLINCVATPPMKLSPAITN
                     DANDFNASSQQIYNRLLAFKAGELTVEPSLAERWEISDDGLSYTFHLRKDVKFHSNKQ
                     FTPTRTLNADDVVFSFQRQMDLNHPYHKVSDGTYFYYHWMNLPKILKTVEKVDDYTVK
                     ITLQQPNAPFLTTLAMDFLSIYSKEYADKLLAEGKPELLDQAPIGTGPFVFQAYQTDQ
                     AVRYVANPDYFQGKAKFEKLIFSITPDAGTRYAKLKAGECDVIDFPNISDIAQMKQDP
                     KVNLLQRDGLNLAYIGLNSTKPALNNAKVRQALHYATDKKAIVEAVYQGGGTVASNPF
                     PDSVLGYNPNLPQYEFNLEKAKALLTEAGFADGFETEIWVQPVVRPSNPNPRRTAEII
                     QADWAKIGVKAKLVTHEWADFNKRTREGEFSTGTYGWTSRNGDPDNFLFPLLSKSNIP
                     GTNYGRWTDEKFEQLLAQAVQTQNRDERAKLYQQAVEIANEQTPFIPLAHAINYVPLS
                     KRVQGFVQNPFGYTPFYGVSLSE"
     gene            26062..26502
                     /gene="mioC"
                     /locus_tag="PXB27_000524"
     CDS             26062..26502
                     /gene="mioC"
                     /locus_tag="PXB27_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HDL5345347.1"
                     /translation="MTSICIITGSTLGGAEYVADHINEVLSEQGFEVELFNQATLADI
                     EGKTNLIIVTSTHGAGELPENIQQLFDDLANSSADFSSMKFGVIGLGSSDYDTFCNAV
                     NIVEEALKAKGASQVCESLRIDVMNNFDHDGSAEEWLPSFVEQL"
     gene            26514..26681
                     /locus_tag="PXB27_000525"
     CDS             26514..26681
                     /locus_tag="PXB27_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HDL5345348.1"
                     /translation="MNKISANKAKWIKIAIILIYMFSPVDILPEAILGPFGLVDDAAA
                     LMLLIKTILEK"
     gene            complement(26770..>26852)
                     /locus_tag="PXB27_000526"
     CDS             complement(26770..>26852)
                     /locus_tag="PXB27_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005715729.1"
                     /note="transcriptional repressor of asnA which codes for
                     aspartate-ammonia ligase; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HDL5345349.1"
                     /translation="IQSIDEIQSTETLISLQNPISRDILP"
BASE COUNT         7830 a         5691 c         5697 g         7634 t
ORIGIN      
        1 tttgcaattt aaattaattg agtgcagaat gccattagcc ttttttgaaa tcacattcca
       61 taaaatggct tgattaacac aacacaatta tcactcaaat taggattttc attatgagta
      121 ttttccaaca aaccaatcca aaccgccgtc gttatgggct agctgtattt atcgggatta
      181 ttgccggtat tatttcagca ttcgtaaaat ggggggcaga acatcctttc ccacctcgta
      241 gccctatcga tctgtttacc gctgcttgcc cgcaacctgt gttagatgca atgaatgccg
      301 cagcagacca agtggctgcc aaaaccatcg cattacaaga atgttcacgt gcatttttaa
      361 acccaccaca cgttttctta cgtgattata ttggtatcga tccaactgaa gcagcattta
      421 cctttgctga ccacgcgttc aactggattg gtgtcaccca tatgattttc tcgttagtct
      481 ttgcgattgg ttactgcatt gtggcggaaa tcttcccaaa aattaaattc tggcaaggta
      541 tcggtgccgg tatcatcgct aatatttgtg ttcactacat caccttcccg gcattaggtt
      601 taacaccacc ggttgctgaa tggccgttat atgaacacat ttctgaatta gttggtcata
      661 ttttctggtt ctggaccatt gaggtgattc gtcgcgattt acgtaaccgt attacgcgtg
      721 aacctgatgc cgaagtgccg ttagatcaac cgttccgtta atttaaaatt agctcgattt
      781 atacaaaagc cgcaatcacg ttgcggcttt cttattttac aagcggtcaa attttcatga
      841 aaatttacca aacaaaaagc acattgtttc atgtagcaat gcggagcttg gctttataag
      901 aaaaatacac caagttggaa cagtttttca atttcatcga tctgtttctt atcgttcaca
      961 aaaataatca cacggtcatt ttctgcaatc atggtggatt tgtgggcgat aataacttct
     1021 tcgcctcgca ccaccgcccc aacaattgcc ccttgcggta gttttaattc acggatttgt
     1081 cgtccaacca cttttgaagt ggttgcatcg ccgtgagcga taatttctac cgctcctgca
     1141 accccaagtt tgagtgaaga aacttgtaaa atatcgcctt ttctaacgta actagcgaga
     1201 gcagaaatag tagcttgttg tggggaaatg gcaatatcaa tagtgccacc ttgaatcagg
     1261 tgtaaatagg cagggcgttg cactaaaata atcacttttt tcgccccaag ccgttttgcc
     1321 agcagggcgg acataatatt cgcttcatca tcactggtga cggcaaggaa taaatcaata
     1381 ttctcaatat gctcctcaaa cagcaactct tcatcggagg catcaccggt aagcaccatc
     1441 gttttagata gtttttctgc taatttttcg gctcgaaccg gattgcgttc gataattttt
     1501 acccgatact gatcttctaa atcccgagct aacgaagagc cgatgctccc tccaccgaca
     1561 atcataatcc gcttatgtgg gcgatcaagc cgttgtagtt cactcattac tgctcgaata
     1621 tgttgggttg cacagatgaa aaagacttca tcacctgcct caataatagt tgaaccttgt
     1681 ggcacaatcg ctttgtcttg gcggaaaatt gccacgaccc gagcatcaat atgcggtaaa
     1741 tgatcacgca gggctgaaat cgggtaaccg accaaaggcc caccgtaata ggcttttaca
     1801 ttcacgatac tgactaactc atcagcaaaa tgagtaattt gcaacgcccc cggatagtga
     1861 ataaggcgta aaatatcttc tttgactaaa atttcagggg caataatatg gtcaataggc
     1921 aatacatcat cattaaacaa ccgctctcgt tctctcacat aatcagcact gcgaatacgg
     1981 gcaattttgg tcggcacatt aaataaagtg taagcaattt ggcaagcgat catattcact
     2041 tcatcactat tagttacagc aactaataga tcagcatcac ctgcaccggc atcacgcaag
     2101 gtttgaggtg aagcatattc gccccgaatc acctgtaaat cgtgtttatc tttgagcttg
     2161 tctaaacgag ttgggtcatt atccaccaag acaatatcat tgtcttcact caccaaattt
     2221 tctgccagcg tagaaccaac ttgtcctgcc cctaaaataa ttactttcat tactctgcca
     2281 ctttttgtag ttttgcgtag aagaaaccat caccaccgtt tggttgtggg aagaactgtt
     2341 tttctaacac tttttcgccg ttaaaatcga tttcaagttg ttttgcattc ggtgtatctt
     2401 gtaaaaaccg ttgaatttgc tcgctatttt cttgcggtaa caccgagcaa gtggcatata
     2461 acaaaatgcc gtttggcttt aacctttgcc ataacgcctt gagaatctca ccctgtaatg
     2521 tggctaattc tgcaatatca ccctctttac gcaaccattt aatatcaggg tggcggcgaa
     2581 tgactccggt tgcggaacaa ggggcatcaa gtaaaatgcg gtcaaacagc acaccctctt
     2641 caagccattc ctcaggtttg gaagcatcgc cacaaattac ctgtgcaatc tgccccagtc
     2701 ggactaagtt ttcacgcact cggcttaaac ggctctcttc aatatccaac gccataacct
     2761 ttgctttcgg tgctttttct aaaatatggg tagttttgcc acccggtgcg gcacaggcat
     2821 ctaaaacggt ttcaccattt tctgccccga gcaactccgc tgaccattgt gcgtgagcat
     2881 cttgcacggt tgcccaacct tgctcgaaat gtggcagttg gttcacattt accgctctct
     2941 ccagcaataa agcacacttt ggcacgcaag cggtcggatt ttctgaattt tttgcaaatt
     3001 caggctcaac cagctcgcta atcaacgccg cataatcatt ggtcttgata tgttgttgat
     3061 tgactcgaat ccacattggt gggcgttggt tgtttgcttc aacaatttct cgccagttcg
     3121 gataggcttt tttaagttta ttgaccaacc attcagggtg gagcgtttgc caatgtttat
     3181 ctaccttcgc taagatttct tcttgctctc gcagaaaacg gcgtagcaca ccgttagtca
     3241 aggctcggaa gctatccagt tttagcgttt tagttgcatt cactacctca tcaaccgcag
     3301 cgtgtgccgg cactcgcata taaagcagtt ggtataaacc cactaacagt aagcaatgca
     3361 ccaaacgggt tttgcctttc agcggttttt caactaataa tttgatgatc gattccaagc
     3421 gaggcagcac acggcacact ccgaaggtga tttcttgcac taagggtaaa tcttttggtt
     3481 caagctgttt ttgagcgtca ggaatcaagg tggaaagtga tttaccttga tctaacacct
     3541 gtaaaataat ggttgcactt attgctctag aagaaagtgc tttgaacgat tgttttttca
     3601 taaataaaaa tgccattccg acagaatggc actattgtaa ctgaatgcgg gattatctta
     3661 aaactaaaat tggcttggtg gtgttgcgag ccactttaac cgtattagag cttaagccct
     3721 tcgcatttgg tttgctgctt gccagcatca taattaaatc caccgacttt tcatcggcaa
     3781 tacggcaaat ttcttcataa atcgtaccat acgccacaat gtgctgcact tttgaacctt
     3841 caggaaaatg gtttttggta aattcgtgca atttttggtt tgcttcagcc accacttctt
     3901 tatcgaaatt tttcggcaag aatgcagaaa ttaagctgtt atccaccggc tcaataatag
     3961 acacaacccg ataaaccgca ttcggatttt ttgccgtcat ttgtaatgcc gtatctacca
     4021 catatttagc actttttagg tcggctaaat caatcgcaat aagtaatttg ttatacatat
     4081 ttattccgta ggggcaggtc ctgtgcctgc ccgaatatta actaaaatag cggacgggca
     4141 caggacccgt ccctacatag catataaata ctatgcaata acacactgtt ttttcactct
     4201 acggcgttgg tttgccacaa taccaaatac caacaataga gcaggaatga agatccaatc
     4261 tttagaagga gcatcttgcg gtacggcaat gtagtgaatg gtttgatccc agtttaagcc
     4321 tgctttagct gccggagaat ccatttctac catatcaatc accacttttg gtgtttctac
     4381 gccatcaact ttgatttttt catcggtttg aattagcgtt aagcctaagt ttttcaaacg
     4441 ctcttcgccg gttgctcctt ccggtacagg taattgagcg tagaactcaa tttctttacc
     4501 gtaagggttt aatccctcta cttttaaagt aacattttga cctactttgc ttgcaagcaa
     4561 ctgttcttca aactgcaccg gctcgatatg acgttcagac ggataaattc tgtccataaa
     4621 gaagcccgga cggaacaagg cgaaagcggc tacaaccagc aacgccgttt cccaccattt
     4681 gtttttagtc acaatatatt gcattgtagc agaggtaaag gcgagaaccc caacagttgc
     4741 ggtaataaac acaacccagc cgtgccccgg ataacgcaga tcaattaata ataaatcggt
     4801 attaaaaata aataagaacg gtaaaatgac ggttcttaaa ctatatttaa atgccaccat
     4861 accggtatta atcgggctac cgcctgaaat tgctgccgca gcgaacgagg ctaaaccaac
     4921 aggtggagtc acatctgcca taatcccaaa atagaatacg aataaatgca ccgcaatcaa
     4981 cggcacgatt aacccgtttt gacgaccgac ttccatgata accgttgcca ttaatgatga
     5041 caccacaatg tagtttgcag ttgtcggtaa gcccattccc aaaattaagc tgaatacggc
     5101 taccaagatc aacattagca caatgttgcc cattgatagg agttcaatca cacttgaaag
     5161 ctgaacccca aagcccgtta gtgccacaac gccgacaata atccctgctg ttgcggtagc
     5221 gagggcgata ccggtcatat tacgaccgcc agcctcaagg ctatctacca catctcgaac
     5281 accttgtttg ataatctctt tggttacgct ctctttacgg aagaaattca gtaacgggcg
     5341 ttgggttaat tgaataaaaa ttaaggtaat ggttccccag aatgcggata agcccggcga
     5401 aagcatttcc accattaagc accaaagtag cacaaccaca ggaataaggt aatgtaaacc
     5461 tgcatttacg gttggttttc tttttggtag agaaacaatt tctgagtttg gatcgtccgc
     5521 ttctaaatca gggtagcttg ccacacggcg aatagccagt agataccata agcctaagaa
     5581 agcacagaca atcataaacg cataatcttc tgccacatca cgaatccaac ctaagccgta
     5641 gtaaacgcca atcgctaatc ctacgatcac taaaattgaa gctacggttc ttagcaagaa
     5701 gactaaaatc gggcttggct cttcggctct tggtaagcct tgtaaattca tcttacaagc
     5761 ttctaaatgc acgatataca ccaacgcaat atacgaaatt aacgcaggta agaaggcgtg
     5821 agtaatcagc tggctatacg gcatattcac atattcgatc atcaagaacg ctgcagcccc
     5881 cattaccggt ggcataattt gaccgtttac tgatgaggca acttccaccg caccggcttt
     5941 ttctttggta aagccgaccc gtttcatcat tggaatagtg aatgtacctg tcgtcacgac
     6001 gtttgcaatc gaagagcctg aaatcaagcc ggttaaagcg gaagaaacca ccgccgcttt
     6061 tgccggacca ccacgcaaat gccctaaata ggcaaaggcg gttttaataa agtagttacc
     6121 tgctcctgct ttatcgagca acgccccaaa taatacgaac aggaacacat atttggtcga
     6181 aaccccaagg gctacaccaa acacgccttc agtggtaatc cattgttggt tgataatttg
     6241 agaaagcgaa cctgagcggt ggcttacaat ccaatctgcc gggaaatatt gcccgaagaa
     6301 gttatagatc aggaatacaa tcgcaataat cacaagcggt aagccaagac tacggcgggt
     6361 cgcttccagc accaataaaa tccctaaaca gccggcgata atatcttgcg tatttggcgc
     6421 tccaaaacgt ttcaccaaac cttcatagaa gaaaatataa tacaacgcta acccagctcc
     6481 tacgatggca aacacccaat ctattttagg aatatgatgt ttaggcgagc tggcaaaagc
     6541 agggtaagat aggaacgcca agaacattgc aaaggctaag tggatagaac gggctttggt
     6601 gtcgtccacc accacattca aattaatgcc tgcgtgagta agccattctt ggaatacaaa
     6661 cggcaatgga gaagcataat aaatttggaa cacagaccaa agaatggcga catacacaat
     6721 ggcttttttc gcaagaccgg acggggttcg ccccccactg tcattggagg caaccatatc
     6781 ttgtaggtca tcgtaatcaa ccttagattc aactgacata aagactcctc aaaacgcaag
     6841 cggtcatttt cttattaaat tttacaaatt tcctcaaaaa acaaccgctt gctcgtgaaa
     6901 acactgttga taagttatca attaaaggtg aaaatcgata acttatcaac agtttcaaaa
     6961 aagcaaaaaa ccagcacgct aaaaaacgtg ctgattgaat caaattacag aaaggaaata
     7021 ttacttaatc caacctttct ctttatagta acgaattgca ccttcgtgta acggagcaga
     7081 aagggcgttt ttgatcatct cttcttgttt taagtgttca aacgccggat gtaaacgttt
     7141 gaaacggtcg aagttatcaa ataccgcttt ggttacattg tacactttat ctgcatccac
     7201 atcggcagaa gtcactaacg ttgcatatac accgaaggtt tctgtcggct catccgaacc
     7261 tttatataaa ccgcccggaa tcgtagcttt ggcataatat gagtgatctg ccactaattt
     7321 atcaatttca gggccggtta ccggcactaa tttcgcatcg caagaagcgg ctgcttcttt
     7381 taacgcacca ttcgggtgac ctacgttata agtgatggca tctaagttgt tatcacacat
     7441 tacagatgcc atttctgccg gtttcaattc agaagccact ttgaaatctt tatccgtcca
     7501 gccttttgcc gctaaaatca cgttcattgt tgcacgagta ccggaacccg ggtcgcccac
     7561 gtttacacgt tttgctttta aatcatcgaa cgatttaatg cctgaatcgg tacgagccat
     7621 tactgtaaac ggttccgggt gaagcgagaa aatggcacga attttatcgt ttttcttacc
     7681 ttcaaatgag cttgagccgt tataggcgtg gtattgccag tctgattgtg caatccccat
     7741 atccatttct tttgctgcaa tcgcatttaa gtttgcaacc gatgcccctg ttgaaggagc
     7801 gttacatttt aaacctgttt ttgctgaatc acggtttact aattggcaaa tagattgacc
     7861 cacaacataa taaacaccgg tttgaccgcc tgttccgata gtcacaaatg tttcagccgc
     7921 ttgaacagat gctgttccca tcacaaggct tgcaaggaaa gaaagtttga ctaatttttt
     7981 catttttaac tcctaaaatt aaggatcaga aaacaaagat ttattgtttt gagccactgc
     8041 tcacactaca agagatacaa aacttctttg agttgcattt gcctcttaga gcttgttttt
     8101 agcatagatt ttataattag taaatttatt ttttcgcata aaaaaatcta tgcctaatgc
     8161 cacaagctaa aatgtttcac tatctcttaa caaatgcaca tcgccttttc gtcttaaaaa
     8221 accgctacga tcaaaatatt caattaattg taccgtcaat tttcgcccat actgcaactc
     8281 atctcgcaac tggttcaccg aaatgctgcc gttttgggcg ataaaatctt taattaacgt
     8341 tgcaaattgc ccgatttgtt cgcttaataa aaatctatct ttgacgattg ggattaaata
     8401 gcccaatttc ccgactttat acagcaaatt ccgcatttgg gtttcatcaa tcgagagctg
     8461 gcttgcaata tccctcaccc aaagagcttg attggttgcc tcaaacagcg gtcgaatttg
     8521 ttgccaaatt tgcaattcat cggcgttcaa ttcgattcga tgttccggca aatgtaacca
     8581 accacgacta ttgaccaagg ctttttgctc caccaaatcc tcaataaatt gatttgccaa
     8641 gttttcaggc aaatccaata acgctatgcg atataaacga gctttggtca cgccgatttg
     8701 atcttgatgt tgcaggtggt attctgccat tttttccaac acccgttgct gggcttgagc
     8761 tttaaaatcc gaattaaata accatttaga cgagacatca aaccccagtt ttgcaatatc
     8821 ctctgcaaaa cattgctccg cccataacag caaggataaa tccatcgctc tgttttgcag
     8881 atagagatca aggcgtgtcg caaagtcatt acaagcggtg gtttttgcta aattttttac
     8941 caaagctaaa cgggcttcgg tacgtttatg ccgttttggt gagtgaattt ccagcacctc
     9001 cgctccggct aacgttaggc tgtcatcacc gctgcggatg atcaacttat caccaacggc
     9061 aatatgaagt ggttcatcta aaatcacttc tgcaaaaaat tgctgatttt tgaccgcttg
     9121 ttgcccctca agcaaattca atttgccggt aatgtgcgaa gcaaaatggt aaagatgcac
     9181 aatgctgttt tctttgaagg cttgattggc agtgagttga accgtaatgc gatcggtggc
     9241 aaatttcggc tcaagctcgg taatccaatc gcctcgcttg atttcctctt tttccacgtt
     9301 agcgatattt aacgccaaac gttgccctgc cactcccgtt tccgaaggcg tattttgggc
     9361 gtgaatcgct ttgacccgca cttttttacc attcgacaaa aataactcag agccaacgct
     9421 cacttttccc gccaccgttg tgccggtaac gaccaaccct gctcctttca cattaaacac
     9481 acggtcaatc gcataacgga acggtttatg ggtttgggta tgttgttgat ttaattgaat
     9541 taaatgtgct tttaattctt caatcccttg ttgcgttttt gccgaagtaa caaaggtttt
     9601 tgcttccgct aaaaacggat agcttgtttt cacttgctca attaagtggc tgatttgagt
     9661 ttcttccgcc cgatctgcct tagtaatcac aatcataata tgctggaaat tcagcaggtg
     9721 gagaatatcc aaatgctctt cggtttgtgg cttaatccct tcttcggcag aaatcaccaa
     9781 cagggcgtgt tgaatgccgc caagccctgc cagcatatta gataaaaaac gctgatgacc
     9841 cggcacatca ataaacccta aaatatcgtt ctcaatcggt aaataggcat agcccaaatc
     9901 aatggttagc ccacgttttt gttcttcggg caaatgggcg gtgttagtgc cggttaaggc
     9961 ttgtaaaagc gatgttttgc cgtggtcaac gtgaccggcg gtgcagatga tcattgatag
    10021 tccttataaa aatgagtaac tattctggcg agcagattta aaatctacaa tatagacatt
    10081 ttcctgacca atcaggaact ttacttggta cttaccgttt aaaatgggaa tttctttttt
    10141 cttgcgaagt cgataaacat cggcagtaaa ggaaagttta tccacttcac tttttattgc
    10201 atcataaaat tgttctgcaa taatcgggtt ttgggattgt tgtaatatat aatctgcttg
    10261 ataaaacaat tttatttctg ctttctgtag ccaaacaata ttgtaatcaa acttcatcat
    10321 ttttccgtga ttcccaattt tttgcgtaaa tcttccatca cctgagcgtg ggatttagtt
    10381 tcaattaagc cattttctaa ttgatgtaat aaatcaagtt catcattagt ataaaaactt
    10441 tgtggcttat ctgtgcgtaa ttttgctaaa tcttgtgcga gtatgagttc catatttccc
    10501 ccttacaaac ttagattatt caatattgta acctgataaa actaaagttc aaggagtgta
    10561 tttaaggcga taaccgctct tttttccact cattattttc caaccaataa tgaatgcggt
    10621 cgtgtagccg gctacttctg ccttgccaga gttcaatact gtcgggaatc agaatatagc
    10681 caccccaatg cggtgggcga ggcactgaaa gtggatattt caatgcaaat tgagctgatt
    10741 ttagcaacaa agccttgtag ttttcgatag gctgactctg ctcgctcgcc caagccccca
    10801 aacggctggt gtaaggacga gagtcaaaat aggcatcgga ttctgcctct ggaatttttg
    10861 ctattttccc ttcaatccgc acttgacgtt gtaattctgc ccaaaagaag gttaaacatc
    10921 caaaaggatt atcggcaatt tcccaccctt tacggctttg ataattggta aagaacacaa
    10981 agccttgtga atttacctct ttcaacagca ccattcggct acttggtcgc ccgtttgctg
    11041 caacggtagc aatattcatt gctgtcggct cattcacttt ggcattgatt gcctcactca
    11101 accattgttc aaattgggtg attgggtttg agtgacaatc tcggattgaa agcacttttt
    11161 tggtgtaatc ttctcgaatg tggtgcagat ccataaggat tcctttatct tattctaaat
    11221 aatcggacag gcacagtctg ccctaccaaa attaatattc caacatctca atcaacttat
    11281 caaactccgc gaccgagcgt aaatctaacc aaaatttctg ctgagcaaaa cgcccgataa
    11341 tcgggcttgg gtaggtttta aattgttgtt ccaatgcaat taaatcgctc tgtttttcag
    11401 cggaaatagt cactgccacc gaaggtaagg tttctgtggg taacgcaccg ctgccgattt
    11461 gagcgaggct tagctcaatt tgcaaaatat aagcagaatt taaccgcttg tgtagtgcct
    11521 gttttaatcg ctctgctttg tgttgcaagg tttcaagcgg ttgagtgagt aaatgaagag
    11581 tcggcaagtt atcggttaat tgctccggaa ataagtagtg gcgtagcgtt gcctcaagtg
    11641 ccgataaaat cactttatcg caacgtagca cccgttttag cgggtgttgt tgcagttggc
    11701 taatcatcgc ttggctaccg acaataatgc cggcttgcgg cccgcctaat aatttatcgc
    11761 cagagaagga aaccaaatct accccagccg ctactttttg ctgaaccttt ggctcagcag
    11821 gcaaattaag agcttgcata tcagttagcg aaccactgcc taagtcggta atcacaggca
    11881 ggttaaactc tttaccgaga gccaccaatt cctcttccga caccgaactg gtgaacccct
    11941 caatgtggta gttactggtg tgaactttca ttaaaaaggc ggtattttcg gtgatcgcgt
    12001 tgcgataatc ctttaaatga gtgcgattag tcgtaccaac ttccaccaat ttgcaaccgg
    12061 cttgagccat aatatccgga atgcggaatg ccccgccaat ttccaccagc tcgccacggg
    12121 aaacgatgac ttccttgcct tgtgcgaagg ttgctaacat caacaacacc gctgccgcat
    12181 tattattcac aatgcaagcg gcctccgctc cggtaagtat ttgcaacaac tcggaaatgt
    12241 agttatctcg gtgcgagcgc ttgcctgctt caatatcaaa ttccaacgcc acattgtttc
    12301 gcattgcgtt ggttgctgcc tgtatcgcct tttccgacca caacccacgc cctaagttgg
    12361 tgtgtaacac cgtaccggtt aagttgaaca cggaacggat tttcacctca ctcaacgctt
    12421 gcactttttg ttccagcctt ataaaaaaca ggttttcatc ggcaataaaa tccggcaagg
    12481 cttgctgttg ggaaataaat tgacgtgctt gagtaagcaa actcctagct tgttcagcca
    12541 cgctggaata tgcaaatttt tggcataatt cgaccgcttg tggctttttc aacaagaggt
    12601 cgagcgaggg aatttggcga aatagttgtt gcataatgtg aatgattgag agaatatttt
    12661 ggacaattct aataaataaa gtacaatatt gccaattttt cggaagtgcg tcatctccgg
    12721 tgaggtggca ggacttcaaa tcctgttgag gacgccagcg ttctcgggtg ggttcaactc
    12781 ccatgcactt ccgccacatt tcaaaagccg ttaatcttta acggcttttt gcatttctgc
    12841 cacattctct ctttccgttt tgataaaaaa tgagacttag ctcacatttt tttatttttc
    12901 tgtttgtagt aaaaatttat taaattataa ttatttggag tattttttca caaacaaaat
    12961 ctagtccatt gactaccttt tgaacataaa aaaggaactg ttatgtcaac actatctcgt
    13021 gattctattt taagtcaaat tcaaaacggg ctgattgcct cctgccaacc ggttgatgac
    13081 ggccccatgg ataaacctga aattgtcgcc gcaatggcag cagcctctgt gattggtggt
    13141 gctaaaggca ttcgcattga aggtgtcgat aatttaaaag ccgctcgtgc ggcagtgaat
    13201 gtgccgatta tcggcattgt aaaacgtgat ttgccagatt cccctgtgcg tattacgcca
    13261 tttttagaag atattgatgc cttagccgaa gccggtgccg atattattgc ggtggacggc
    13321 acattccgca cccgcccggt cagcattgaa gaggcggtaa aacgcattca cgaaaaaggt
    13381 tgcttggcaa tggcggattg ctccaccctt gaagaaggtt tacattgcca acaattaggc
    13441 tttgatattg tgggtagtac aatgtctggc tacaccggtg gcgaaatgcc tgatgagccg
    13501 gactatcaat tagtgaaaga tttaaaagct gccggctgta atgttatggc ggaaggtcgc
    13561 tacaacacgc ctgaactggc aaaaacggcg attgaaatcg gggcggatta cgttaccgtt
    13621 ggatcggcat tgacccgctt agagcatatt gtgagctggt ttgccgactc tgtaaaatcg
    13681 gctcaaaaat aggagctgct tatgcgttgt ttagcaatag acatcggtgg cacgaaaatt
    13741 gccacggcaa ttgtgcaaca caatcaagtg tcacaacgcc aacaaattca gaccccgact
    13801 gataaaccac aagctgatca ggcaggcagt ttgcatcaag cgattggtga gataatgcaa
    13861 cattatcaag ggcaatttga ttttgtggca gttgcctcaa caggcattat caataaaggg
    13921 gtgcttaccg cattaaatcc taaaaattta ggcggattgg cagaatttcc gttgaaacaa
    13981 agcattgaaa aacataccga taagccgatt gcattattaa atgacgtgca agcggcggtg
    14041 tgtgccgaat atttaaatga agaccaacaa gcggtcaaaa attttgtatt tattaccgtt
    14101 tccaccggcg taggcggcgg cattatttta aacggcgaac tgcaaatcgg cagtaacggc
    14161 attgcaggac atatcgggca tactctagcc gatccgaacg ggcctctctg tggttgtgga
    14221 cgagtcggct gtgtcgaagc agtcgcagca gggcgtgcaa tagaggcagt ttccagccaa
    14281 tgggaaaagc cttgttcacc gaaagaggtg ttcgagcgat tccgccaagg caacccacaa
    14341 gcggtcgaat tagtacaaaa atctgcaaaa gcgattgcta atttaattgc agatttaaaa
    14401 atcagtttag atatacaaaa agtggtgtta ggtggcagtg tgggcttagc ggaagggtat
    14461 ttaccgcagg tgtccaacta tttagctgaa atgccgagcg tatatcattg cgtattggaa
    14521 aatgctctat ccgggcaaga tgccggctta atcggtgcgg cttggtgggc ggaaaaccaa
    14581 cttaatcaag gttttactct ttaacaaatt ctaactccgg agatccaaat gaaaaactta
    14641 aaaggtattt ttagtgcgtt actcgtctct ttcaacgaag acggcaccat caacgaaaaa
    14701 ggcttacgcc aaattatccg ccacaacatc gacaaaatga aagtggacgg tttatatgtc
    14761 ggcggttcaa ccggtgaaaa ctttatgctt tctaccgaag agaaaaaaga aattttccgc
    14821 attgccaaag acgaagcgaa agatgaaatc gcccttatcg cccaagtcgg tagcgtaaac
    14881 ttaaaagagg cggtagaact gggtaaatat gccacagaat taggctacga cagcttatct
    14941 gcggtcacac cgttctacta caaattcagc ttcccggaaa tcaaaaattt ctacgaaacc
    15001 attattcgtg aaaccggcaa caaaatgatt gtttactcca ttccgttctt aaccggcgta
    15061 aacatcggtg tgagccaatt cgctgaatta tttgaaaacg aaaaaatcat cggtgtaaaa
    15121 ttcactgccg gcgatttcta cctgttagag cgtttacgca aagcgttccc gaaccactta
    15181 atctacgcag gtttcgatga aatgatgcta ccggcaacgg tattaggtgt ggacggtgca
    15241 atcggctcaa ccttcaacgt aaacggtatt cgtgcaagac aaatctttga attagcccaa
    15301 caaggcaaaa tcaaagaagc atttgaaatc caaaatgtaa ccaacgattt aatcgaaggc
    15361 attttaggca acggtttata ccaaaccatc aaaggcttat tggagttaga aggtgtgcaa
    15421 gcaggttact gccgtgagcc gatgacgaaa gaacaaactc cggcacaaaa agaagtgacc
    15481 aaacagttaa aagctaaatt cttgtaattt aagttgcaaa aggctaaacg aaaatcaccg
    15541 cttgtagtaa caagcggtga ttttttctca acgacttgca attaatgaag ccattctgct
    15601 ttttctacca cggtgtaacg tgtcaccatt atattttaat cgcacttttt cttggcgagt
    15661 attttctgat ttcaactctc tacctatatc ataaaaccct gtactccaga tattttgagt
    15721 aatatccgct tttacaataa tatcattgaa gccgttggtc tgtttgttat ccatttgcaa
    15781 taccaagttt gtgtttccgt tttggtttct ttatcatcta aaatagacca aaaagtcatc
    15841 gggtaatcaa aaacaagttt taacttttct ttttccggca caaataacat caaatgatca
    15901 tttgcagaat actcaggagc acttggacca cgagcagcat tattaaacct cacgccgaaa
    15961 gctcttactt tgtcgtttag aatataacgg gcggtatcaa ttttaagact atctgcattc
    16021 acatcgatag aggcatcgat ctccatatcc tgctcccacg attgcaatat cgcattatca
    16081 ttcacacgag caacaaaaag cgtccactta ggtgtgtttt cgtcgttttg ataattttct
    16141 ttgctgtatt taggctctat taccgcataa ctcactaagg tttccgttga attgttagga
    16201 gctgttttac aggcagaagc taaagtacga gcagaaagtt tctcttgaga aatcttaaag
    16261 taatgagcaa taatttgatg ttgttgctca gcacaaacct gtgtgttatt accttcatct
    16321 gcttgagttg caagcgtaag taaaagaact aagaggagta tctttttcat atagatttat
    16381 ccgtatagaa agataactca ttctatgggg tactgactct ttttgcatag agagaaagaa
    16441 aaaaatttgg tcggaaaaat aacggcgagc ctagcgaaaa caagggttcg ccgtaatttt
    16501 atgggggaag ctatgtaaaa aacaaaatga gcaggattag aaaaaaatga gcagtttttg
    16561 cgatattttt tgcataggtt tgatcggttt taaaactatt ttaaatctgt tttaaactaa
    16621 agagtgtccg atgtggacta cctgcccgat gatttggagg ttttgagctt cttcggcggt
    16681 aatctgtatt gggcggtagg actcgttatc gctaattaat tcaatatagt cccattcttt
    16741 tctaatccgt ttaacccata cgctttcccc attctgcaca agataaatct tatcatcacc
    16801 acgaggctct cgtttgctga aatctaccag catttggtca ctgtctgcaa tggtagggtg
    16861 cattgatgtt ccatttgccc aaaacacgcc gcagttgtgg gcttttacgc cgaggctttg
    16921 cagcaggctg tcggcgtaag gttcttgccc gtctggcttc gttacgccct cgttaaagct
    16981 gccaaagcct gctgaaacgt tgattgttct gaagctgtta atcattgaaa caatatcatc
    17041 agccattttt tctagtcgct caaactctct tgccacaata ctggtattct ctgattgtgg
    17101 tttgctttcc ttacctgttg ccagccattc aaggctcaca tttgacacct ctgctatttt
    17161 tacaagattt gaacgagatg gatctgcctc tccttttacc cacctagata gtgatgcttg
    17221 ggctaccccc actgctcttg caaactcact gtaattgtcc ttaaagctat tttttgcaat
    17281 ccatttcatt ctttctgaga agctctcatc gtaaatgttt ggtttactca tcttttacgt
    17341 ttccattaat aaagttttac tcgcaaaaca tactcataaa ccattgattt ttaagttatt
    17401 tatgaataaa gcaaaaatat tttagatgtt tttatgtttt acggttgatt tgtttaaagt
    17461 tttacggtta aatacacgca tcaaatagat aaaaagaggt gtatcgaatg agtgtattaa
    17521 acgatgttaa aaaaaccgca cagagagatt ggcataaagc cgatattctt gcggaacttc
    17581 gtaaaaaagg ttggtcactt cgttctttgg ctaaagcagg caatgttagc cataacacgc
    17641 tgaagtctgt tttagacaag ccttacccca aaatggaacg cttagttgca aatgccatag
    17701 gggttgcccc tgaagtgatt tgggctgggc gaacagcaga acgcattgaa cgtaatcggc
    17761 gaccaacttt aactaataag ttttaatcat aaatgatctt taacgtaaaa gatataggag
    17821 aagaacattt atgagcgaat taagtttaaa aacgcactat tcagtggctg aattgttaag
    17881 ttttaagtta tctagcctac caagtgctca taaaaatgtg cttgagaaag caataaggga
    17941 aaactggcaa tctcaaaaac gcaaaggcag aggcggcggg gttgaatatg aattaatcag
    18001 tttacccacc gaagtccaac aggaaatccg caccaagtta ctcaaacttc ttcctgctga
    18061 aatcagcaaa ggcgaattga gtgtagtgcg acaaaacatt gatttggagc agataaccga
    18121 taaacaactt tctacggcag atgctcgcat tatggtggtg cggtggttct tgatgcagga
    18181 agtgcagttg gggctaagcc gcacaaaaac gctagaccaa gtcattgcag cggtggcaag
    18241 cagtgaaatt cctgctgaaa tttgcaaagc gattatggcg ggcaatagta aagccggtgg
    18301 aaagttaaag ctatctaaac gcaccttgca tagttgggtg ctggcgtatg aggcaggcga
    18361 aaattcagcg gaacggttaa aacagatgat accgctgaaa acacaaaaac gtgccgtgcc
    18421 cgagcggtgt ggttggttgc aagcctttct gccgttttat caaaccttta gcaatgtggc
    18481 actcacacag gcttatgctc aatttgcaat gcagtatgag ggcgaagatt tacctacaga
    18541 gagccaagtt cggtatgcct tgaagcagtt gcccgattat gtggtgcaac aagggcggaa
    18601 attgccggtg aagaagatcc ggctcgcccg ttaaataccc ttgaagttaa aggcttaacg
    18661 gtcgtagatg actcgcagaa accgttattt agcgaagtaa accaagcgtt atatcacggt
    18721 ttaagcccga ttgaggtggt ggcaagccgt gtgcagatta cccgtgcgat tactacatac
    18781 accaaaaacg tgacgaatac tgatgaccca agctatttgg atttaaccac cattcgtacg
    18841 cttgactatg tgcgtaaggc aatccaaacc cgtcagcgtt tgcgtttccc tcgtgcgaaa
    18901 aactcccacc gtattgtggc aaaagtacgc tcggagattt tagatgtgct ttatcagctt
    18961 gagggcgaag aggtaatcga aaatgtggaa gcgtggaaaa atcgcttatt agtggtgcgt
    19021 aatcaagacc cgacttactt ggatttagaa atccctgcgg atgtggttaa tggcttgcac
    19081 gttattcgta acaaaattac gttaatttta tagggggtgc tagatgtcaa ttgtttatga
    19141 aggctcttgc gtgcttgagg tagatggcgt tgagattgaa attaccaaac ttgatgtaaa
    19201 aaccgtaacc ggtcgcaagc tggttaaaac gatgaacagt tccggacgtg caagaggtta
    19261 ttcgcaaggc attgcggaat atactctctc ggtgactgca gttgagcctc aagacggttc
    19321 ggtaattgat tgggataaca ttaaaaatgc gaagctcact aaatacccgt tagacaatag
    19381 cgaaaaacgc acctcttatt taggctgttt tactaccgat gtgagcgagt cttacaccgt
    19441 tgatggtgag tcggtgcgtg atattcaact caacgcattg cgtaaggtga atgaataatg
    19501 aaaatcactt taaatcttgg cattgaatat aacgggaaaa ttcgccgtga ttgtacggtg
    19561 cgattgctga cgatgggcgg tcaatgcacg gctcaagaaa tgattgcgga tttagggttt
    19621 gaggaaaact cggaagccct atcaaagcgt gaacaaacct tggtcgatat ggcttacctt
    19681 tcgcagcaag ttacctttga tggtatttat caaggtaatg tgacggctca atttttattt
    19741 gacaatttag ctaccgaaga ctactggcag ttgattgagg caacgctcac actccgaaaa
    19801 aagcggttag aaaatggggt gagccaaagc gagagcgaaa gcccggcaaa tcccgatttg
    19861 gcgtagcaga agcctttaag cagtaccgac aagcggtgat tttactctca aaatttgcaa
    19921 ttaccgccga gcaggtatgg caaatgccgt tgcttgaggt atttgcgtgg attgagagct
    19981 acttggagag tgagggcgta aaaacaccgc atagcccaat gggtgcgaat agcgaagtga
    20041 aacgcgaaag ctatgtcttt actcggtgtg gcaaaattga ataggggcga aagccccttt
    20101 tttgtacttt aaaattagtt taaagagact ttaaaaatgg caaaaaatat gaaagtgcaa
    20161 ctggaattga cggcaaaaga tactgcctcc ccagttttaa accaagccgc taaaaccgcc
    20221 gaaaaagcct ttaaaaatac agagcgagcg gcacaaaaaa gtggcaatgc tcaaacgcaa
    20281 gcagcacaga aagcagcgaa tgctacagaa agctcaaatc ggaaaataga acagagttac
    20341 cgtaatgccc gccgagtggc tgccgatgtg agccgagccc gtgaaacatt aggtgttcgg
    20401 gcggaaaatg caatccaacg cgaaattgaa caaactcgtg cagcctatga ccgcttaaaa
    20461 cgctcaggtg ttgcctctca aaatgagcta cgccgtgcct cagaagccac taagcaacgg
    20521 gtgcgagagc tgaatgcaga attgggtaag acaagctttg gggataaggc taaaggattt
    20581 gggcgtggtt tgatgagtgc cggtgtcggt attgcagcag gtgctgcaat ggctcgtcag
    20641 ccagccaaga accaaatgga gtttgaccga cagttagcga tggcatcaaa cactgccttt
    20701 tccgaccgtg atgttaaagg gcgtattgag ggcaagaaag agttattagc agcggtgcaa
    20761 gaggcggtta aggttggggg tggaacaaaa gaagatgctc ttttaactct tgatacgcta
    20821 attgcctcag gaacgatgaa ggctgaaact gcaatgagcc tattaccaac cttgcagaaa
    20881 ggtgctgtag caacaggtgc aagctccgag gatatggcaa aaattgcgat ctcctcaatg
    20941 caacaatttg ggattaaaga agaggatatt gggcgggcat tggatatggc agttgcagcc
    21001 gggcaagcag gtaactttga gcttgcagat atggctcgct ggctaccaca acaaatggcc
    21061 gcaggtaaca cggcaaggca ttgggcggtt tattaccgcc tgtatcgtat gacccaaacg
    21121 gcagatggct agcgttatcc cttgaagttg aggggagaga gctagaccgc attgatgcga
    21181 gagccaagac tttaatcgat gccgttgatg tatcaacagg catttttatt gatgattggg
    21241 agcgggtttg tggcttaacg gcgaatagct cactaccgct tgaaacccga attgagcgtg
    21301 tgattgcaaa gctgaatcag ttgggtggat tgtcgatagg ctatatcact caagtggcca
    21361 aagagcttgg atattgcatc aaaattagtg agccacaacc ctttattgct gggctaagcc
    21421 gagctgggga tatgttatgg cataaagaca ttatgtggac attttttgtg gatgtttgca
    21481 accccacaga tgattacaag cgttttagag caagcgtgtc aatggctggc gatgcgttaa
    21541 tgggacgata caaagaccca attttagaaa gtctacttga agaggtaaaa cctgcattta
    21601 gccgtgtgtg ggtaagatat ttaggagata ttgaatgaaa caagtgatga atccgattaa
    21661 taccccaaca cagcgtttta aagatggcaa ccctgccact ggtgaatatg gcacgattgt
    21721 gactgctgaa tttttgaata acgtccaaga ttcggtcatt aacacccaac aagaattgca
    21781 cagtgtactt gctgaagcag gaatcgaagc aaatgacgaa caagttaatc aggtggctaa
    21841 agcaatcaag aaaattgcgg gcgatgctac tcgtgataat tttaacgcat tagccaaccc
    21901 tgatggttac aaacacatcg gtcgctgcaa atcggttgcg gagttacgta ctatccgccc
    21961 aacggaacac ggtcagcgta ttttggtgga tgcttactat gaggacggca caacaggcgg
    22021 tggggagttt gtggctgatt tgcaggattt agttacgcct gatgatggtg gggtttgttt
    22081 tgttgtgaat aataatgggg ggcggtggaa gcgtgttgac ctttcacatc ttacattatt
    22141 tgattttggg gccgtcggtg acggggtaac aaatgatgaa tcagcatttg taaatgctat
    22201 gagatattct caatttttta tcgaaaacgg tacatttcgt atcaacaatg ccgttaattc
    22261 tgttcgcgat aatgtgcaaa ttttgggcaa taagaccggc aaattagtat taggtgcagg
    22321 tatccagcaa gcagaggccg aagtttttaa tattaatcac agcaattatt ttatctcggg
    22381 tttttgtatt gaaacaccga acaaagcaat tggtattcga tttaagtctt tggatgatgc
    22441 tggtgttaaa aatctccata ttgacaatgt tgtgtttaat ggcaccttct acggggtcag
    22501 ggcaggtgaa agtattcagg cggatactaa ttacccaact gataatgtga ttgtacaaaa
    22561 ctgccaatcg tattgtggtt caggtaatgc tggacattat ctctgcacca aagttaaagg
    22621 tgtgcgattt tttaacaata tagcaattgg aggtcgcaat gtatcagctt acggtgcaac
    22681 atcttgctct gatattttca ttttgggtaa tcgtgaacag gggatggcag tgacgacagt
    22741 tgatgtggaa gcgggcgtac aagtggaaga tgtacaaact ggcgaatgtg tggtagcgaa
    22801 taatatttgc agccacgata tttggatgtc tggtgctaaa aatgccatta tcaacagcaa
    22861 cctctgccgt cgcttgcgtg tcacatcagg taatccgact gacacaggca gcaacaatgt
    22921 agaattttca aataatcaag caaaatctat tgcaatatcg aaatatggtt ctttcgataa
    22981 tccttcattg tttattgata atatcaattt tattaataac attttggagc ccagtgaatt
    23041 taatcaagat gtgtatattg acgggacaat tttaaaacag tgccaattta ttaataatcg
    23101 ttgtattagc aagtctaagc aatattctgt cagacttgct cgcaataatc cgtgcttaaa
    23161 tttaacattt aagagtaata aattatcgga caatcctgcg attatatcag gtaagcaagg
    23221 tgttattttc tgcgatgatt tgagcttagg agtaaataag cacagattaa gattgcgatt
    23281 ttcatcgccg attaatttta ctgttaatca agaatggcaa aaaatgccgt ttgacagagt
    23341 gttaaatgat atcaatggtc atgttgatat tgtcagtggt caatttattg caaggacagg
    23401 cggcactttt catttaaaag gtaaaataac ggttacaaat tcatcggaag atgtgctgaa
    23461 tttatatcaa ctcaggctgt taataaataa cgaagagaaa gaaagactga ttaatcaaag
    23521 tgtttcacct aaaaacagca aatcaaatac aaatggtatg acaacaatca attttgatac
    23581 tgtagtttat ttaaaagtag gtgacattgt tgatttgcag tataaaacaa cgcatgagtt
    23641 gacattactg tcagactcat ttttatctca aatttatgtg actcaggagt tttaagtgac
    23701 taaaaaacac tactgcacag gctggcgtaa tgcgccagcc aatatcaata gttgctgtca
    23761 tcagcatgac cgagactacg gtatcaaggg cacagtcagc cgagccgaag ccgacaagcg
    23821 gttaagacat tgcttaattt cgcaagggaa tccgattaag gcgtgggtgt tttgggttgt
    23881 ggtgaggttg tttgggtgga tttattacaa ggagaaaaat atcaatggaa agtcataaaa
    23941 caggaaatat cattactgag caagcaagca agcaagcaag caagcaagca agcaagcaag
    24001 caagcaagca agcaagcaag caagcaagca agcaagcaag caagcaagca agcaagcaag
    24061 caaagtgtag ctcaatttaa acaagctccg ctacctttta caggtcaaaa gcggatgttt
    24121 ttaaaacact tttaatgatg atttaaaggg cgtttgtttt cagtttattt tgcataactt
    24181 ttgtgattac ggctatgcaa aatgatttgc aaaactatgc aaaataaatc gaccgcttac
    24241 aattctaaaa gaaaagcagt ctattctcta ccacattttg caaaaaagtg gcaaaaatag
    24301 accgcttgta tggcatatca ctccgataaa ctcaccccat aaaatggcgt gtagccaaac
    24361 ggattttgca caaaaccttg cacccgtttg ctgagtggta cataattgat agcgtgagcc
    24421 agcggaataa atggcgtttg ttcgttggca atttccaccg cttgttgata gagcttcgcc
    24481 cgctcgtcac ggttttgggt ttgtacagct tgagctaata actgctcaaa tttctcatcc
    24541 gtccaacgcc cataatttgt tcccggaata ttagatttgc ttaataaagg gaagaggaaa
    24601 ttatcaggat caccattacg gcttgtccaa ccataagtgc cggtagagaa ctcgccttca
    24661 cgggtgcgtt tgttaaaatc cgcccattcg tgggtcacta atttcgcttt caccccgatt
    24721 ttcgcccaat ccgcctgaat aatttcagcc gttcgacgtg ggtttgggtt agaagggcgc
    24781 actaccggtt gtacccaaat ttccgtttca aatccatcgg caaagccggc ttctgttaat
    24841 agggctttgg ctttttcaag gttaaattca tattgcggta aatttggatt atagcccaac
    24901 accgaatcag ggaacggatt acttgccacc gtgccaccgc cttgataaac agcctcgaca
    24961 atcgctttct tatcggttgc ataatgcaag gcttggcgaa ctttggcgtt atttaatgcc
    25021 ggtttggtgc tgtttaagcc gatataagct aaattcagcc cgtctctctg taacaaattc
    25081 accttcggat cttgtttcat ttgggcaata tcggaaatat tcgggaaatc aatcacatcg
    25141 cattcacccg ctttcagttt ggcataacga gtgcccgcat caggggtaat actaaaaatc
    25201 agtttctcaa attttgcttt accttggaaa taatccgggt tcgcaacata acgcaccgct
    25261 tgatcggttt gataggcttg gaacacaaaa ggccctgtgc cgatcggggc ttgatccaat
    25321 aactccggct tgccttccgc cagcaattta tccgcatatt cttttgaata aatcgacaag
    25381 aaatccatcg ctaaggttgt cagaaaagga gcattaggtt gctgtaaggt gattttaaca
    25441 gtgtaatcgt ccactttttc cactgttttt aaaatcttcg gcaaattcat ccaatggtaa
    25501 tagaaataag taccgtcaga gaccttatgg taagggtggt ttaaatccat ctggcgttgg
    25561 aacgagaaca ctacatcatc cgcattcagc gttcgagttg gcgtaaattg tttattggaa
    25621 tgaaatttca cgtctttacg taaatggaat gtgtaactta agccatcgtc actgatttcc
    25681 caacgttccg ccaaactcgg ctcaaccgtt agttcaccgg ctttaaacgc taataagcga
    25741 ttataaattt gttgtgaact ggcgttgaaa tcattggcat cattagtaat tgcaggacta
    25801 agtttcatcg gtggagttgc cacgcaatta atcaacgtat ttaccggagc tgctgccacc
    25861 aaactgctgg cacttaagag taaaacagaa agagataatt gagaaagacg cataaagact
    25921 ccttaacatt ttctactaac ttactcggca ttaacgtatt ttcaaaataa ctaaaggtta
    25981 taatttataa tcagaataat cagttgcaaa ccaatgattc ttggctagaa tattggcagt
    26041 tttttaaacg agagatttac tatgacatca atttgtatca tcaccggcag caccttaggc
    26101 ggtgcggaat atgttgccga tcacattaat gaggtattaa gcgaacaagg ttttgaagtg
    26161 gaactgttta accaagccac ccttgccgat attgagggca aaaccaatct gattatcgtt
    26221 acctctaccc acggagccgg cgagttaccc gaaaatatcc aacagctatt tgacgattta
    26281 gcaaatagct cggctgattt cagcagtatg aaatttggtg taatcggttt aggtagctcc
    26341 gactatgaca ccttctgcaa tgcagtaaat attgttgaag aagctctcaa agcgaaaggg
    26401 gcaagccaag tttgcgaatc actgagaatt gatgtaatga ataatttcga ccacgatggc
    26461 agtgctgaag aatggttacc atcatttgtt gaacaattat aacttaagta tcaatgaaca
    26521 aaatcagtgc aaataaggca aagtggatta aaatcgctat tatcttaatc tatatgttca
    26581 gccctgtaga tattctaccc gaagcgattt taggcccatt cggtttggtt gatgatgccg
    26641 ccgcattaat gctattgatt aagactattt tggaaaaata ggcatagtag acaaaatagt
    26701 tgcaaaaaac caaaacaaaa cgaccgcttg taacccaata caagtggtct tatcttttac
    26761 ttaattcttt taagggagaa tatctcttga aataggattt tgaagtgaaa taagcgtttc
    26821 tgttgactga atttcatcaa tagactgaat tt
//
LOCUS       DANLUQ010000015        25769 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.015, whole genome shotgun sequence.
ACCESSION   DANLUQ010000015 DANLUQ010000000
VERSION     DANLUQ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 25769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25769
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.015"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            52..1029
                     /gene="manX"
                     /locus_tag="PXB27_000527"
     CDS             52..1029
                     /gene="manX"
                     /locus_tag="PXB27_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HDL5345350.1"
                     /translation="MSIAIIIATHGVAAEQLLKTTEMLIGEQEDVAYIDFVPGENAET
                     IMGKYQALVEGPLSHCDQVLFLVDMWGGSPFNAANRFQDGKSGMDVVAGVNIPMLVNT
                     CMARDDVESLEELVEVALEAGSTGIRSLNHQPEVAEVQTATPAQETAAVQQPEIVDDG
                     RRMTIALARIDDRLIHGQVATVWTKESKVNRIIVVNEGVAKDSVRTTMLKSVAPLGVT
                     AHVVDVPKMIRVYNNPEYADDRVMLLFTNPSDVLALIEGGVPLNSINVGGISHKEGKQ
                     MVSHSVAVDQNDIDAFKAIDAKGVELEVRKVSSDSRQYMMDLLKKHNLI"
     gene            1041..1841
                     /locus_tag="PXB27_000528"
     CDS             1041..1841
                     /locus_tag="PXB27_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005716709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HDL5345351.1"
                     /translation="MELSALQIVLLFLVACISGMGSILDEWQTHRPLIACTMIGLVLG
                     DVTTGVIVGGSLELLALGWMNIGAAVAPDAALASIVSTILVIVGRQDISTGIAIAIPL
                     AAAGQVLTYVVRAITVGFQHAADKSIEDGNLSRLDWLHRGALLLQAMRIAIPALIVAL
                     TAGTDAVQTMLNAIPEVVTTGLKIAGGFIAVVGYAMVINMMRAGHLMPFFFAGFVIAG
                     FTDFNLVALGVLGTVIAMVYIHMHPKYNKSQQVVVAAAPKNDLDNRLD"
     gene            1855..2691
                     /locus_tag="PXB27_000529"
     CDS             1855..2691
                     /locus_tag="PXB27_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005564636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HDL5345352.1"
                     /translation="MTTEIKKVTKSDLNAVVRRSNLFQGSWNFERMQALGFAYAMVPV
                     IKRLYPDPNGPGRKEAIKRHSEFFNTQPFVGAPILGVTIAMEEERANGKEIDNAAING
                     IKVGLMGPLAGVGDPIFWGTVRPVFAALGAGLALKGELLGPILFFALFNIVRLAARYY
                     GVVYGYKKGLDVVQDMSGGLLQKLTEGASILGLFIMGALVQKWTSINVPLVLSTIQKQ
                     DGTIEVTTVQSILDSLMPGLLPLLLTFACMWLLRNRVNAIWIILGFFVIGILGAATGA
                     LA"
     gene            2784..3269
                     /locus_tag="PXB27_000530"
     CDS             2784..3269
                     /locus_tag="PXB27_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HDL5345353.1"
                     /translation="MTNTILILFILLGLVFAIYDQVFMHKLKGTTLLEIRLKKQKTID
                     TWLLIGLIVLSISYGVQNQIQPFTLYLLATCIILSVYLAFFRSPRLLLKQHGFFFANV
                     FFNYDKIYQVNIAEGKADEKILVIDLHSGRRLLAYVENAEDLQPVVDFFGGYKKESEQ
                     K"
     gene            complement(3355..4146)
                     /gene="tpiA"
                     /locus_tag="PXB27_000531"
     CDS             complement(3355..4146)
                     /gene="tpiA"
                     /locus_tag="PXB27_000531"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDL5345354.1"
                     /translation="MARRPLVMGNWKLNGDKAFTKTLIADLKAELHGVEGCDVAIAPP
                     VMYLAHAEASLAGCGCGCSDKGLIALGSQNVDVNVKGAFTGDISPEMLKEFGAKYIII
                     GHSERRTYHKESDEFIAQKFKVLKDAGLVPVLCIGESEAENEAGKTEEVCARQIDAVI
                     NAHGVEAFNGAVIAYEPIWAIGTGKSATPAQAQAVHKFIRDHIAAKSQAVADQVIIQY
                     GGSVNDANAAELFTQPDIDGALVGGVSLKAPAFAVIVKAAAAAKA"
     gene            4306..5325
                     /gene="truD"
                     /locus_tag="PXB27_000532"
     CDS             4306..5325
                     /gene="truD"
                     /locus_tag="PXB27_000532"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HDL5345355.1"
                     /translation="MKLNYLYSPPQAVGRLKAEYADFIVREELGYELAGEGEFVAVKI
                     RKTNASTLFVGEKLAKFVGISEREMSYAGLKDRHTVTEQWFCLHLAGKETPDFSAFEC
                     EGVEVLEVTRHNRKIRVGSLAGNHFELLLRDVAESEELIYRLQQLQAVGFPNYFTEQR
                     FGRDGHNLTQALRWANGEISVKDRKKRSFYLSAARSEVFNLVVSQRINDCLTQKILEN
                     DYVQLAGSNSFFCVKSDEIAETQARLAQQDLLLTAPLIGERSLELECNPAEKAIIEQH
                     AQLVALMQKERMAAARRAMLCKPQNLEWQFEQEGLRLKFFLDSGSYATALVRELILLT
                     EESEA"
     gene            5496..6284
                     /gene="surE"
                     /locus_tag="PXB27_000533"
     CDS             5496..6284
                     /gene="surE"
                     /locus_tag="PXB27_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HDL5345356.1"
                     /translation="MRILISNDDGFHAVGIQTLAKALRDAGHIVTIIAPDRNRSAASS
                     CLTLVDPLRVHRFDEYNYAVIAGTPADCVHLALNGLFDGKFAEKFDLVVSGINHGANL
                     GDDVVYSGTVAAALEGRHLPLPSIAVSLVGRKHSSFLDGENHFGTAAQVVLDLLEKME
                     TQRFPSNHVLNVNVPNLPYSELKGTMITRLGERSPAAEIVKQKDPRNADVYWIGVTGE
                     PLDESFGTDFYALNRDCVSITPIQVDMTAHKTIATLKGIFNETV"
     gene            6271..6855
                     /locus_tag="PXB27_000534"
     CDS             6271..6855
                     /locus_tag="PXB27_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDL5345357.1"
                     /translation="MKLFETIYDKTMQWSKHRFAAFWLSFVSFIEAIFFPIPPDVMLI
                     PMSMAKPQNAFKYAVYTTLASVAGGVIGYFIGLYATDWVQGVIANWGMQANFDKAKLW
                     FETWGVAVVFLAGFSPIPYKVFTICAGVMQMAFFPFVITAAISRFARFILVAKLSAWG
                     GKKYEQKIRRSIELIGWGTIVLAVVAYIIYTLMK"
     gene            6873..7373
                     /locus_tag="PXB27_000535"
     CDS             6873..7373
                     /locus_tag="PXB27_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL5345358.1"
                     /translation="MKKSLFVLSLISFALVGCSSNSGESTSAVETTEANATWQSADSI
                     QTAPMPASMNQPIATQPTYQQPTYSAPQPISAAPAPMPVSSGSDSSQVETVGNCQVVR
                     DANNAPIYSQITKGCYTESHYTVGKSDTLYLIGYLTGTNANQIAQLNGLNTGSPLKVG
                     QVLRVR"
     gene            7386..7586
                     /locus_tag="PXB27_000536"
     CDS             7386..7586
                     /locus_tag="PXB27_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345359.1"
                     /translation="MNRTLLAFAISCAFLTACSSPTQPQVEDPNMLPAGTLQPIEGSG
                     ASQDSYSWPSDIQSTSMPDSMK"
     gene            7605..8885
                     /locus_tag="PXB27_000537"
     CDS             7605..8885
                     /locus_tag="PXB27_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDL5345360.1"
                     /translation="MKKQFILLPLVSAVLAACSSNNPAPVVNASGNNELSPGVMQPVD
                     VSAGAINSSGGWQSDIQSAPMPSSMNTTPVQTMPQPVSVPQPVATVPQPITNPVITEQ
                     PPQPTTTTKIVKKTKTVEKKVDQNFEIPRDANNAPMYNQIQKGFYDGSTYTVRKGDTM
                     FLIAYIVGKDVKEIAALNNMSEPYQLSVGQKLKTGKSATETITVEEKVTVPVEPQITY
                     QQGANGTTYASGGNITGPVKAGTANADVTPVNNGIKATTATAPAVTASVGTVNATEDR
                     TASTIRAIDGTASGAVQSSNTVSAPASSIKWQWPTSGRVISGFSSAEGGNKGIDIAGN
                     KGQDVRAAAAGKVVYAGNALQGYGNLIIIKHTDDFLSAYAHNNTIEVDEQETVKAGQK
                     IATLGSTGTNTNKLHFEIRYKGKSVDPARYLPRK"
     gene            9126..9317
                     /locus_tag="PXB27_000538"
     CDS             9126..9317
                     /locus_tag="PXB27_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345361.1"
                     /translation="MDPTEPSHRIYILLSEDRFQAEVFSTKLSESSMLLEAVKGGYIS
                     KDGKVRLLKKAEGWKIYYE"
     gene            9310..9876
                     /locus_tag="PXB27_000539"
     CDS             9310..9876
                     /locus_tag="PXB27_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDL5345362.1"
                     /translation="MSETKIRCDWCKSSEIYMKYHDEEWGKPEFDSLKLFEKICLEGQ
                     QAGLSWITVLKKREAYRQAFHQFNPEKIAKMGEEEIDLLMQNTSLIHHRAKLEAIIKN
                     AKAYITMQQNGEDFSRFIWSFVNYQPQINDVPTLEAVPAHTETSKAMSKALKKRGFVF
                     VGETTCYAFMQSMGLVNDHLNNCVFKHK"
     gene            complement(9923..10153)
                     /gene="acpP"
                     /locus_tag="PXB27_000540"
     CDS             complement(9923..10153)
                     /gene="acpP"
                     /locus_tag="PXB27_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005725021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDL5345363.1"
                     /translation="MSIEERVKKIIVDQLGAKAEDVKPEASFIEDLGADSLDTVELVM
                     ALEEEFDIEIPDEEAEKITTVQSAIDYVQNNQ"
     gene            complement(10340..11017)
                     /gene="rpe"
                     /locus_tag="PXB27_000541"
     CDS             complement(10340..11017)
                     /gene="rpe"
                     /locus_tag="PXB27_000541"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDL5345364.1"
                     /translation="MSKNPYLIAPSILSADLARLGDDVAEVLKAGADLIHFDVMDNHY
                     VPNLTFGPAICKALRDYGIQCPIDVHLMVKPVERLIPDFAKAGADYITFHPEATEHID
                     RNLQLIRDLGCKSGLVFNPATPLSYLDYVMDKVDVVLLMSVNPGFGGQSFIPSTLDKL
                     KEVRRRIDESGYNIRLEVDGGVKINNIADIAAAGADMFVAGSAIFDQPDYQAVIAQMR
                     DELRKVG"
     gene            11211..11483
                     /locus_tag="PXB27_000542"
     CDS             11211..11483
                     /locus_tag="PXB27_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005709555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDL5345365.1"
                     /translation="MKKGIHPENYREVLFYDASVQQGWVIRSCAATNKTMVWEDGKEY
                     PLYTLDTSSASHPVYTGKRREVNTEGRASQFKDRFKGFASTLSNQK"
     gene            11492..11617
                     /gene="ykgO"
                     /locus_tag="PXB27_000543"
     CDS             11492..11617
                     /gene="ykgO"
                     /locus_tag="PXB27_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HDL5345366.1"
                     /translation="MKILNSLKTAKSRHPDCQIVRRKGKLYVICKTNPRFKARQR"
     gene            11797..12570
                     /locus_tag="PXB27_000544"
     CDS             11797..12570
                     /locus_tag="PXB27_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL5345367.1"
                     /translation="MLKKLSIAALALTLSVSAFAKESLLERINNKGTITVGTEGTYAP
                     YTYHDASGKLTGYDVEVTRAVAEKLDVKVEFKETQWDAMLAGLKGERFDLVANQVALT
                     SPERRATFDKSESYSWDGAALLVREDETKIKTPADVKGVKTAQTLSSNYGEIARENGA
                     DIVPIDGMAQGLLLIQQKRADATFNSHLSLLDYLKKNPNSGLKIVWEKEEKVGAGLIA
                     NKGNDEALAKISKAIVELREDGTLKKLGEQFFGKDISVQ"
     gene            12653..13369
                     /locus_tag="PXB27_000545"
     CDS             12653..13369
                     /locus_tag="PXB27_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL5345368.1"
                     /translation="MLNDLLLSIPFMTQARVDLVLSGFWPMVQAAFLVSIPLAIASFI
                     IGMLIAVAVALVRISPATGFFHRLLLWLVKGYISIIRGTPMLVQICIVFYGLPAIGIF
                     IDPIPAAIIGFSLNIGAYGSETIRAAISSVPKGQWEAGYTIGMTYMQTFRRIIAPQAF
                     RVAVPPLSNTFIGLFKDTSLASVVTVTEMFRVAQQVANMSYDFLPVYIEAGLIYWCFC
                     WVLFLIQARLEVRFNRFVAK"
     gene            13379..14131
                     /locus_tag="PXB27_000546"
     CDS             13379..14131
                     /locus_tag="PXB27_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL5345369.1"
                     /translation="MLKIRNIHKTFGNNAILRGIDLDIQKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEQGTLEFSGNAPLKIDFAVKQSKKEILALRRKSGMVFQNYNLFPHKTAL
                     ENVMEGPVFVQQQAVEIAQKNAKALLEKVGLADKAELYPFQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLNTMKELANEGWTMVVVTHEIKFALDVADVVVVMDG
                     GEIVEQGSPQQLFNHPQHERTKRFLNQIRADK"
     gene            complement(14347..14880)
                     /locus_tag="PXB27_000547"
     CDS             complement(14347..14880)
                     /locus_tag="PXB27_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDL5345370.1"
                     /translation="MEFLAPVISLIVGFLSFVLVLRTWLQFCRVDPRLPISQTLLKIT
                     EPMVSPVGKLIPTVKNICFPALLIAAALVTLQFILFGVDLPRAVLIGLLSVVKTFGQI
                     LFFTTLIRALMSWVTQGNHPLDYTVAQITEPVLGFIRKLLPRTGMLDFSVMVLGFGLL
                     LLNSLFYQIFGGLWAAA"
     gene            complement(14986..15327)
                     /locus_tag="PXB27_000548"
     CDS             complement(14986..15327)
                     /locus_tag="PXB27_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HDL5345371.1"
                     /translation="MAIQRNARQRKKMHLGEYQELGFLIKFTFAEGTGIDQIDETVDR
                     FIDEVIRPNGLALEANGYLNWEGLVCLEKLGKCDESHRQLVKTWLETNGLKEVQVSEL
                     FDIWWDYPAQA"
     gene            complement(15351..16100)
                     /gene="trmB"
                     /locus_tag="PXB27_000549"
     CDS             complement(15351..16100)
                     /gene="trmB"
                     /locus_tag="PXB27_000549"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDL5345372.1"
                     /translation="MSEKQTFADKKRKTVEQAEFTEDGRYLRKVRSFVLRTGRLSDYQ
                     RDMMNNNWANLGLDYQPMPFNFEQIFGNNNPVVLEIGFGMGKSLVEMAEQNPNRNYIG
                     IEVHTPGVGACIAYALEKGVKNLRVICHDATEILRDSIADGTLGGLQLYFPDPWQKAK
                     HHKRRIVQPEFISRVIRKLSPNGFIHFATDWENYAEHMLEVLRQFESELQNTSATNDF
                     IPRPNFRPLTKFEERGHRLGHGVWDLYFTKK"
     gene            complement(16110..17060)
                     /locus_tag="PXB27_000550"
     CDS             complement(16110..17060)
                     /locus_tag="PXB27_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDL5345373.1"
                     /translation="MNSRILATGSYLPTKVRTNADLEKMVDTSDEWIVTRSGIRERRI
                     AEPDETVATMGAKAAQQALEMAGIEANDIDLIVVGTTTNSHAYPSAACQIQGLLQIED
                     AIAFDVAAACTGFVYALTVADQFVRNGQVKKALVIGSDINSRMLDETDRSTVVLFGDG
                     AGAVILEASEELGIISTHLHASPDLENNLTLRSQKRGEERSGFIEMQGNATFKIAVNQ
                     LSSVVEETLEANNLQKSDLDWLIPHQANLRIITATAKKLEMEMDQVVVTLDKYANTSG
                     ATVPVALDEAVRDGRIQRGQLLLLEAFGGGWTWGSVLVKF"
     gene            complement(17206..18225)
                     /gene="hemB"
                     /locus_tag="PXB27_000551"
     CDS             complement(17206..18225)
                     /gene="hemB"
                     /locus_tag="PXB27_000551"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDL5345374.1"
                     /translation="MQVIQSQYPLRRMRRLRKHDFSRRLVAENQLTVNDLIYPVFVIE
                     GENQRVQVPSMPGVERLTIDQLLVEAAELVKYGVPAVALFPVVGDVKKSLMAEEAYNP
                     QGLAQRAVRALKAQFPELGVITDVALDPFTTHGQDGIIDETGYVLNDVTTEVLVKQAL
                     SHAEAGADIVAPSDMMDGRIGKIREALEQNGFINTQIMAYSAKYASNYYGPFRDAVGS
                     AGNLKGGNKYTYQVDPANGNEGLHEVAMDIQEGADMVMVKPGMPYLDMVWRVKENFGV
                     PTFAYQVSGEYAMHMAAIQNGWLKERECIMEGLLCFKRAGADGILTYFAKRVAKWLYE
                     EAQAK"
     gene            complement(18306..19061)
                     /gene="tatC"
                     /locus_tag="PXB27_000552"
     CDS             complement(18306..19061)
                     /gene="tatC"
                     /locus_tag="PXB27_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HDL5345375.1"
                     /translation="MSQVDESQPLITHLIELRNRLLRSFICVLLVFCGLVYWANDIYT
                     LLATPLTDNLPDGATMIATNVATPFFTPIKLTIVVSVFLSVPFILYQIWAFVAPALYK
                     QEKRLVYPLLVSSTLLFYCGVAFAYYVVFPLVFGFLTSTAPEGVTMATDISSYLDFVL
                     TIFMAFGICFEVPVAIILLCWAGVTTPEDLKAKRPYIIVAAFVIGMLLTPPDIFSQTL
                     LAIPMCLLFEVGVLFARFYRPKDDDEPSEQVAQ"
     gene            complement(19048..19692)
                     /gene="tatB"
                     /locus_tag="PXB27_000553"
     CDS             complement(19048..19692)
                     /gene="tatB"
                     /locus_tag="PXB27_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HDL5345376.1"
                     /translation="MFDIGFSELVLIMIVGLVVLGPKRMPIAIRTVMGWVSTIRGLAA
                     NVQNELAQELKLQELKESIKKAEELNLSSLSPELNKTVEDLKMSATQLQDSLNSAKNE
                     LEKTAAESSTIPKLTDEQVAEIQQKIEQESQVLEAQELQKNAQNKPLEVDSLSEQLNV
                     TRTEKPLSADELAEQAEMDESLLTLESYYPPDDDLATPQSSESAQKSEANNVAS"
     gene            complement(19696..19917)
                     /gene="tatA"
                     /locus_tag="PXB27_000554"
     CDS             complement(19696..19917)
                     /gene="tatA"
                     /locus_tag="PXB27_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HDL5345377.1"
                     /translation="MGGISIWQLLIVVAIIVLLFGTKKLRTLGSDLGESVKGFKKAMA
                     DEKKDAEFEKVEAKDAISAEQKAKEKEQA"
     gene            complement(20028..21665)
                     /gene="ubiB"
                     /locus_tag="PXB27_000555"
     CDS             complement(20028..21665)
                     /gene="ubiB"
                     /locus_tag="PXB27_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HDL5345378.1"
                     /translation="MNIKNLNRFYHIIRTFLRYGIDEAIPNLPITRKIKLGRKALFWV
                     KNQHSDKPYGVRLRLALQELGPVWIKLGQMLATRRDLFAPELADQLALLQDNVEPFDG
                     KIARQIIEQALGGKLETWFENFDETALASASIAQVHTAKFNQNQPLAGKDVVLKVIRP
                     GIEPVIKADLELMYEIAHFIPKLSNDGKRLRAVEVVREYEKTILDELDLRKEMHNAIR
                     LRANFENSEMLYVPEMYQDFCHKNLIAMERIYGIPVSNIAELEANSTDMKLLAERGVQ
                     VFFTQVFRDSFFHADMHAGNIFVNREHPENPQYIGIDCGIVGTLNHNDKRYLAESFVA
                     FFNRDYRRVALMHVESGWTPPDTDIDKFEQAFREVCEPIFAKPLSEISFGHVLLNLFN
                     VAREFNMEVQPQLVLLQKTLLYIEGLGRQVYPQLDLWQTAKPFLQNWLNEQVGVKAMV
                     RDIKQRLPQYREHFAEFPEALFKALQEQKQINFRLAEINESLKAQNSNANSRFLVLSI
                     SAIVICGAFWKFESLPIWLSVPLLTLFPLVLILSQRK"
     gene            complement(21739..22716)
                     /locus_tag="PXB27_000556"
     CDS             complement(21739..22716)
                     /locus_tag="PXB27_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005825116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HDL5345379.1"
                     /translation="MKQIIAQARENAVRAVDFQRVLMYWHIGKRIFEQEQQGQERADY
                     GTYLIKMLAKELEPEFGSGFCSRQLERYRQFYRAFPIATALRSQLNWTQYRRLIQISD
                     SDKREFYLAESCKNNWSSRQLERQINSSLYERLLLSNDKESVLAVARNEQQPVDPKQL
                     IKDPMVLEFLGLKRESAYYEKDLEQAIITHLQDFLLELGNGFSFIARQKRLHLDGDDF
                     FVDLVCYNRLLQCFVIFEIKTHKLTHQDLGQLQMYVNYFDRVEKLPHENATIGVLLCA
                     DKNDAVVKFSLPENQNKIFASQYQLYLPSEQTLIAEVQKEIENFEQKYP"
     gene            complement(22877..23524)
                     /locus_tag="PXB27_000557"
     CDS             complement(22877..23524)
                     /locus_tag="PXB27_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HDL5345380.1"
                     /translation="MLNNVKQQLMLPQFTFGLIETAFNALLKRSPHIEPNLRKLSGKV
                     LKIALTSPKIDFFVLFSESRTDWLGNYEGEADCTVQLAFDTLPKLADKQKLTELINNK
                     SLVLTGDIQVLQHFTALLEQLEKDPAELLSPFVGDVVAQTSTDLAKKLLGKVKAQFEQ
                     NNQHLVENLIQERPVLVHRLQAVHFYDQVEELAQQALKLEQKFTHYVRSSSHQQS"
     gene            complement(23715..24467)
                     /gene="ubiE"
                     /locus_tag="PXB27_000558"
     CDS             complement(23715..24467)
                     /gene="ubiE"
                     /locus_tag="PXB27_000558"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HDL5345381.1"
                     /translation="MQQSAETTHFGFKTVAKEEKQQLVANVFHSVAGKYDLMNDLLSF
                     GIHRVWKRFTIDCSGVRKGQKVLDLAGGTGDFTAKFSRIVGESGEVILADINSSMLEV
                     GREKLRNLGVVGNVSYVQANAECLPFADNTFDCIVISFGLRNVTDKDKALRSMFRVLK
                     PGGRLLVLEFSKPIIDPISQLYNFYSFNILPKVGEVVVNDAESYRYLAESIRMHPKQD
                     ELKAMMEQTGFESVNYYNLSAGIVALHRGYKF"
     gene            24812..25765
                     /gene="corA"
                     /locus_tag="PXB27_000559"
     CDS             24812..25765
                     /gene="corA"
                     /locus_tag="PXB27_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HDL5345382.1"
                     /translation="MIRAFGLDNACLISVDETNPTLLNDAIWIDLIDPSEAERTVLEN
                     RLDQTLAEERELEDLEASARFFEDEDGLHLHSFFYCLDDDDYADIATVAFTIRDGRLF
                     TLRDRDLPAFRLYRMRSRREKLIDSNAYELLLDLFETKIEQLADVIEEVYADLEKLSR
                     VILNGQQENENFNDALSRLTEFEDASSKVRLCLMDTQRALSFLLRKTRLPNNQLEQAR
                     DIMRDIESLQPHNESLFQKVNFLMQAAMGYINIEQNRIMKFFSVVSVMFLPATLVAST
                     YGMNFEFMPELHFQYGYPMAIGLMIVAASAPYLYFKRKGWL"
BASE COUNT         7439 a         5577 c         5197 g         7556 t
ORIGIN      
        1 aaaatctcgc cccagtaaat aaacaactta aaaaaccgca aggagtaaca aatgagcatt
       61 gctattatca tagcaaccca tggtgttgct gcagagcaac ttctcaaaac aactgagatg
      121 ttgattggtg aacaagaaga tgttgcttac attgactttg ttcccggcga aaatgcagaa
      181 acgattatgg gcaaatatca agcattggtt gaaggtccac tatcacactg tgatcaagtg
      241 ctgtttttgg tggatatgtg gggaggaagc ccattcaacg ccgcaaaccg attccaagat
      301 ggaaaatcgg gtatggatgt tgttgcgggt gtgaatattc ctatgttagt gaacacctgt
      361 atggctcgtg atgatgtgga gtcgttggaa gaattggtgg aagtcgcatt agaagccggc
      421 tcaaccggga ttcgttcact taatcaccaa cctgaagtgg ctgaggtgca aaccgctact
      481 cctgcacaag aaactgcagc tgttcaacag cctgaaattg ttgatgacgg acgtcgtatg
      541 accattgccc ttgcccgtat tgatgaccgc ttgattcacg gtcaagttgc gacggtttgg
      601 acaaaagaaa gtaaagtcaa tcgcattatt gtggtaaacg aaggtgtagc aaaagatagt
      661 gtacgaacta ctatgctaaa aagtgttgct cctcttggcg taacggctca cgttgttgat
      721 gtacctaaaa tgattcgtgt ctataacaac cctgaatatg ccgatgatcg tgtaatgtta
      781 ctctttacca acccaagtga tgtcttagcc ttaattgaag gcggtgtacc actcaatagc
      841 attaacgtgg gcggtatttc gcataaagaa gggaaacaaa tggttagcca ttctgtggcg
      901 gttgatcaaa atgatattga tgccttcaaa gcaattgatg cgaaaggcgt tgaattagaa
      961 gtacgcaaag tctcaagcga tagccgtcag tatatgatgg atttattgaa aaaacataac
     1021 ttaatctaaa ggaaattact atggaacttt cagcattaca aattgtgctt ttattcctcg
     1081 ttgcctgtat ctcaggtatg ggctcaattc ttgatgaatg gcaaacccac cgtccgttaa
     1141 ttgcctgtac gatgataggc ttagtgcttg gtgatgtgac taccggcgtt attgttggtg
     1201 gctcattaga gttattagct ttaggttgga tgaacatcgg tgccgctgtt gcaccagatg
     1261 cggcattagc ctctattgtc tcaaccattt tagtcattgt aggtcgtcaa gatatttcaa
     1321 ctggtatcgc catcgctatt ccgcttgcag ctgccggtca agtattaacc tatgttgtgc
     1381 gtgcaatcac cgtaggtttc caacacgcag cagataaatc aattgaagac ggtaacttaa
     1441 gccgtttaga ctggttacat cgtggtgctt tattgcttca agcaatgcgt attgccattc
     1501 cggcattaat tgtggcatta acagcaggta cagatgcggt tcaaacaatg ttaaatgcca
     1561 ttcctgaagt ggttacaacc gggctgaaaa ttgccggtgg cttcatcgcc gtggtgggtt
     1621 atgcgatggt gatcaatatg atgcgtgcag gtcatttaat gccgttcttc ttcgcaggct
     1681 ttgtaattgc aggctttacc gatttcaatc ttgttgccct tggggtatta ggtacggtta
     1741 ttgcaatggt ttatatccat atgcacccta aatataacaa aagccaacag gtagtggtgg
     1801 cagctgcacc aaaaaatgat ttagacaacc gtttagacta agagggcatt taaaatgaca
     1861 actgaaatca aaaaagtaac aaaaagcgat ttgaatgccg ttgtacgtcg ttcaaactta
     1921 ttccaaggtt cgtggaactt tgagcgtatg caggctcttg gttttgcgta tgcgatggta
     1981 ccggttatca aacgtttata tccggatcca aatggcccgg gtcgcaaaga agcaattaaa
     2041 cgccattctg agttctttaa cacccagcca ttcgtcggtg caccgatttt aggggtaacc
     2101 attgctatgg aagaagaacg tgccaacggt aaagaaatcg acaatgccgc aattaacggt
     2161 attaaagttg gtttaatggg accattagcc ggtgtgggtg acccaatttt ctggggaact
     2221 gttcgccctg tatttgccgc attaggagca ggcttagcct taaaaggtga gcttttaggc
     2281 ccaatcctgt ttttcgcctt atttaacata gtgcgtttag ccgcacgcta ttatggcgta
     2341 gtgtatggct ataaaaaagg gttagatgtt gtacaagata tgagcggcgg tttattgcaa
     2401 aaattaaccg aaggtgcttc aatcttaggt ctgtttatta tgggggcatt ggtacaaaaa
     2461 tggacgagca ttaacgttcc gcttgtgctt tcaaccattc aaaaacaaga cggtaccatc
     2521 gaagttacca cagtgcaaag cattctcgac agcttaatgc cgggcctact cccattatta
     2581 ttaacctttg cttgtatgtg gttacttcgt aatcgagtaa atgctatctg gattatcctc
     2641 ggtttctttg ttatcggtat cctcggcgct gcaaccggtg cattagctta attgcaaaat
     2701 tttctgtgaa aacgaccgct tgaacttgaa cattcaagcg gttttttata cgctttagcc
     2761 caaataaatt cacttgctta attatgacca ataccatcct tattctcttt attttacttg
     2821 gcttagtatt cgccatttat gaccaggttt ttatgcacaa actcaaaggc acaaccttac
     2881 tggaaatccg tttaaaaaag caaaaaacaa ttgatacttg gttattgatt ggtttaatcg
     2941 tcttgtcaat tagctatggc gtacaaaatc aaatccagcc ttttacactt tacctgctgg
     3001 cgacttgcat tattctgtcg gtctatctcg cctttttccg ttcccctcgc ttgttgttaa
     3061 aacagcacgg ttttttcttt gcgaatgtct tttttaatta cgacaagatt tatcaagtca
     3121 atattgcaga aggaaaagca gacgaaaaaa ttttggtaat tgatttacat agcggaagaa
     3181 ggctgcttgc ctatgttgaa aatgcggaag atcttcaacc ggtagtggat ttttttggcg
     3241 gttataaaaa ggaaagtgag cagaaatagt ttgcgaacaa aacacaagcg gtcatttttc
     3301 cctgaaattt tgcaaaaaac tgaggaaaaa tgaccgcttg taaaatcggt taaattaggc
     3361 tttagcagcc gctgccgctt taacgatcac cgcaaaagca ggcgctttaa gtgaaacacc
     3421 gcctactaat gcaccgtcaa tatccggttg agtgaataac tctgctgcat tggcatcgtt
     3481 tacagagcca ccgtattgaa tgatcacttg gtcagccact gcttgcgatt tagctgcaat
     3541 atggtcacga ataaatttat gcactgcttg tgcttgtgcc ggagtcgctg atttgccggt
     3601 accgattgcc caaattggtt cgtaagcaat aactgcaccg ttgaatgctt ccacaccgtg
     3661 cgcgttgatg accgcatcaa tttgtcttgc acacacttct tcagttttac ctgcttcatt
     3721 ttcggcttca gattcaccga tacataatac aggcactaag ccggcatctt ttaatacttt
     3781 gaatttttga gcaataaatt catcgctttc tttgtggtaa gtacggcgtt ctgaatgccc
     3841 gataataata tattttgccc caaactcttt taacatttca ggtgaaatat caccggtgaa
     3901 tgcccctttt acgttcacat caacgttttg tgaaccaagt gcgattaagc ctttatcact
     3961 gcaaccacag ccacaaccgg caagtgatgc ttcagcgtgt gccaaataca tcaccggtgg
     4021 agcgattgct acatcacagc cttcaacgcc atgtaattct gcttttaaat ctgcaattaa
     4081 ggttttagta aacgctttat caccgtttaa tttccagtta cccattacaa gtggacgacg
     4141 tgccattttg tttctcctat atgaagttga acttaaatat aaacgttgca aactatacca
     4201 aattttaatg tcgttttgtt gtttttattt tgctttttct gatctagatc gaaaggttta
     4261 acgtataatg ccgaaatctt attttttatg ttattcgaga agtgaatgaa actgaattat
     4321 ttatactctc ctcctcaagc tgttgggcga ctaaaagccg aatatgcaga ctttattgtg
     4381 cgtgaagaac tgggctacga actagccggc gaaggggaat ttgtggcggt taaaatccgc
     4441 aaaaccaacg ccagcacgct gtttgtgggg gaaaagttag ccaaatttgt cggtatttcc
     4501 gagcgtgaaa tgagttatgc aggcttaaag gaccgccaca ccgtgactga gcaatggttc
     4561 tgcttacact tggcaggcaa agaaacgccc gatttctccg cctttgaatg cgaaggcgtg
     4621 gaagtgctgg aagtgacccg ccacaaccgc aaaattcgtg ttggttcgct cgccggcaat
     4681 cattttgaat tattgctacg tgatgtagca gaaagcgaag agttgattta ccgtttgcaa
     4741 cagttgcaag cggtcggatt tcccaattat tttaccgaac aacgtttcgg gcgagacgga
     4801 cataacctga ctcaagcctt gcgttgggca aacggggaaa ttagcgtgaa agatcgcaaa
     4861 aaacgcagtt tctacctttc cgctgcccgc tcggaagtgt ttaatttagt ggtttcacag
     4921 cggattaatg actgcttaac ccaaaaaatc ttggagaatg attacgttca acttgccggc
     4981 tctaacagct ttttctgcgt gaaatcggac gaaattgctg aaactcaagc ccgtttagca
     5041 cagcaagatc tacttctcac cgctccgctg ataggcgaaa gatcgcttga attagaatgc
     5101 aatcctgctg aaaaagccat catagagcaa cacgctcaat tggttgcctt aatgcaaaaa
     5161 gagcgtatgg cagccgctcg gcgagcaatg ctttgtaagc cacaaaatct ggaatggcaa
     5221 tttgaacaag aagggctacg gttaaaattc tttttagatt caggcagtta tgccactgca
     5281 ctggtgcgag agttaattct gcttaccgaa gagagcgaag cttagtccat aacacataac
     5341 aagcggtaag attttaatca ttttttgcaa tttgcaaaac tttaacaaaa tcttaccgct
     5401 ttttgcactt ttagactaaa aaaactgtcg aaaaattgtt agaattgcga tctttgtatt
     5461 tagagtctat caagatgtaa gatctaaagg aaattatgcg aattttaatt agtaatgacg
     5521 atggttttca cgctgtcggc attcaaactt tagccaaagc cttgcgagat gcaggccata
     5581 tcgtcaccat tattgcaccg gatcgtaacc gcagtgcagc ttcaagctgc ctaaccttag
     5641 tagatccact tcgggtgcat cgttttgatg aatataatta tgcggtgatc gccggcacac
     5701 ctgccgattg cgtgcattta gccttaaacg gcttgtttga cggcaaattt gcagaaaaat
     5761 ttgatttggt ggtctccggc attaatcacg gggcaaattt aggcgatgat gtggtttact
     5821 ccggtacggt agcggcagca ttggaaggcc gccacttgcc attaccaagt attgcagtat
     5881 cactggttgg gcgtaaacac tcttcgtttt tagacggtga aaatcatttt ggaacggcag
     5941 cacaggtagt gttagattta ttggaaaaaa tggaaacgca acgttttcca tcaaaccacg
     6001 ttctcaatgt taatgtgccg aatttacctt attcagagct aaaaggcaca atgatcaccc
     6061 gcttaggtga acgttcaccc gctgcggaaa ttgtgaagca aaaagatcct cgtaatgctg
     6121 atgtctattg gattggtgta acaggtgaac cactggatga aagttttggt actgattttt
     6181 atgcattaaa ccgagattgc gtttctatca caccaattca agtggatatg accgctcata
     6241 aaacgatagc aaccttaaaa ggcattttta atgaaactgt ttgaaacaat ttatgacaaa
     6301 acaatgcagt ggtctaagca ccgcttcgct gccttttggt tgagttttgt cagttttatc
     6361 gaggcgatct ttttcccgat tccgccggat gtgatgttga ttccgatgtc gatggcaaaa
     6421 ccgcaaaatg cgtttaaata tgcagtttac actacccttg cttcggttgc cggtggtgtt
     6481 attggttatt ttattggctt atatgctacc gattgggtgc aaggcgtcat cgcaaattgg
     6541 ggaatgcaag caaactttga taaagcaaaa ttgtggtttg aaacttgggg tgtcgcggta
     6601 gtatttttag ccggtttttc gccgattccc tataaggtat ttaccatctg tgccggtgta
     6661 atgcagatgg cgttcttccc gtttgtgatt actgctgcga tttctcgctt tgcccgtttt
     6721 attttggtag caaaactctc tgcttggggt ggcaaaaaat atgaacaaaa aattcgccgc
     6781 tcaattgaat taattggttg gggaacaatc gttttagccg ttgttgccta tataatttat
     6841 acattaatga aataaatttt aaggatataa aaatgaaaaa atcacttttt gttttatctc
     6901 tgatttcttt tgctttagtg ggttgttcaa gcaattcggg tgaatccact tctgcggtag
     6961 aaacaacaga agcaaatgca acttggcaat cagcagatag cattcaaact gcaccaatgc
     7021 ctgcctcgat gaatcaacct attgccactc agccaaccta tcagcagccg acttactctg
     7081 caccacaacc aatttcggca gcaccagcac caatgcctgt ttcaagtggt agtgatagct
     7141 ctcaagttga aacagtaggg aactgccaag tggtgcgtga tgcgaataat gctcctatct
     7201 attcacaaat taccaaaggc tgctacacag aaagtcacta tacggtaggt aaaagtgata
     7261 cgctttattt aatcggttat ctaaccggca ccaatgccaa tcaaattgcg cagctaaatg
     7321 gtttaaatac cggctctcca ctgaaagtcg ggcaagtctt acgtgttcgt taataggcag
     7381 agaaaatgaa cagaaccctt ttagcatttg caatttcttg tgcatttttg accgcttgta
     7441 gctcgcctac tcagccacaa gttgaagatc caaatatgtt acctgcaggc acattacaac
     7501 cgattgaagg ctcaggcgca agccaagaca gttacagttg gccgtctgat attcaatcaa
     7561 cgtcaatgcc tgacagtatg aaataatttt aggacaatca tttaatgaaa aaacaattta
     7621 ttcttctccc tttagtctct gctgttttgg cagcctgttc ttcaaacaat ccagcaccgg
     7681 tggtgaatgc ttccggtaat aacgaattaa gcccgggcgt gatgcagcct gttgatgtga
     7741 gtgcaggtgc aattaattca agcggtggct ggcagtctga tattcaatct gcaccaatgc
     7801 caagctcgat gaataccact ccggtgcaaa caatgcccca gccggtttca gtgccacaac
     7861 ctgtggcgac tgtaccgcag ccaattacca atccggtgat taccgaacaa ccaccacagc
     7921 cgacaaccac gaccaaaatc gtgaaaaaaa cgaaaacggt cgagaaaaaa gtggatcaaa
     7981 attttgagat cccgcgtgat gccaacaatg ccccaatgta caaccaaatt caaaaagggt
     8041 tctacgatgg ctctacctat accgtacgca aaggcgacac aatgtttttg attgcctata
     8101 ttgtgggtaa agatgtaaaa gaaatcgccg ctcttaataa tatgagcgaa ccttaccaat
     8161 taagcgtagg gcaaaaactc aaaaccggca aatctgcaac ggaaactatt accgtggaag
     8221 aaaaagtcac cgtaccggtt gaaccacaaa ttacctacca acaaggtgca aacggcacaa
     8281 cctatgcttc tggcggtaat attactgggc cggtaaaagc aggcacagcc aacgctgatg
     8341 tcacgccggt taataacggt attaaagcaa caactgcaac tgctccggca gtaactgctt
     8401 ctgtcggtac agtaaatgca acagaggacc gtaccgcctc caccattcgt gctatagatg
     8461 gcacagcaag tggtgccgta caaagtagta atactgtttc tgcaccagct tcatcaatta
     8521 aatggcaatg gccaaccagc gggcgtgtaa tttcaggctt ctcctcagca gaaggaggta
     8581 ataaaggcat tgatattgcc ggcaataaag gacaagatgt acgtgctgcc gccgcaggta
     8641 aagtggtata tgccggtaat gccttgcagg gttacggtaa cttaatcatc atcaaacata
     8701 ccgatgattt cttaagtgct tatgcacaca acaatactat tgaagttgat gagcaagaaa
     8761 cggtgaaagc cggtcagaaa atcgcgactt taggtagtac aggaaccaat acgaataaat
     8821 tacattttga aattcgttac aaaggaaaat ctgtcgatcc ggctcgttat ttaccaagaa
     8881 aataagagta actttttagc ataatccgac cgcttgtgta ctacaagcgg tcaattttta
     8941 ggaaaattat gcaaatgaaa aaatatatcg cacacatgat aagttcgtta ctgctgatag
     9001 cttgttcgga ctaccgagca acaccaacta gcccaagcga tgcttacact tgctctcctt
     9061 cttttcgcta ctctttctta aaaaaaacgt gcataccttt attcagtata gccgacattc
     9121 aattattgga tccaaccgag ccttcacatc gcatttatat tttgttatct gaagatcgat
     9181 ttcaagccga agtcttctca acgaagctct ctgaatcttc tatgttattg gaggcggtta
     9241 aaggaggcta tatttcaaaa gacggaaaag ttcgtttact caaaaaagct gaaggttgga
     9301 aaatttacta tgagtgagac taaaatacgt tgtgattggt gtaaaagcag tgaaatttat
     9361 atgaaatatc acgacgaaga atggggaaaa ccggagtttg acagcctcaa actgtttgag
     9421 aaaatctgcc ttgaagggca acaagccgga ctttcttgga ttacggtgtt gaaaaaacgg
     9481 gaagcctacc gccaagcatt ccatcagttt aatcctgaaa aaattgccaa aatgggtgaa
     9541 gaagaaattg atttgttaat gcaaaacact agcttaatcc accaccgagc aaagttagaa
     9601 gccattataa aaaatgcgaa agcttatatc acaatgcagc aaaatggcga agacttcagt
     9661 cggtttattt ggtcttttgt gaattatcag ccgcaaatta atgatgtacc aacacttgaa
     9721 gctgttcctg ctcatacaga aacctcaaaa gcaatgtcaa aagcgttgaa aaaacgtggg
     9781 tttgtgtttg taggcgagac tacttgctat gcttttatgc agtcaatggg gctggttaat
     9841 gaccatttga ataattgcgt gttcaaacat aaataaacac aaaaaattag gcgaccattt
     9901 ggtcgcctaa cattaattca gattattgat tgttttgaac gtaatcaatt gctgattgaa
     9961 ctgtagtaat tttttcagct tcttcatctg gaatttcgat atcgaattct tcttctaaag
    10021 ccattactaa ttcaacagtg tctaaagaat ctgcacctaa atcttcaatg aatgaagctt
    10081 ctggttttac atcttctgct ttcgcgccaa gttgatcaac aatgattttt tttacgcgtt
    10141 cttcaatgct cattttgttt ttcctatagt gaatatcgca taagtgcgag agttaaatag
    10201 tgtaatcgaa ataagtttaa atacaacctt ttataaaaag gtcaaaccag actattccta
    10261 tacaccagaa tatatctgtt tttccattct atcattatgt ttgtgtttaa tctatagaga
    10321 taaatcaaat ttcatttgat taccctactt tccgtaattc atctcgcatt tgtgcaatca
    10381 ctgcttgata atctggctgg tcaaaaatcg cagaacctgc cacaaacata tctgctcctg
    10441 cggctgcaat gtcggcaatg ttatttattt tcacaccacc atcaacctct aagcgaatgt
    10501 tataaccgct ctcatcaata cgacgacgga cttctttcaa tttatctaag gtggacggaa
    10561 taaatgattg tccgccaaag cccggattca cagacatcaa taaaaccacg tcaactttat
    10621 ccatcacata atctaaatag ctcaatggcg ttgccgggtt aaacaccaag cctgatttac
    10681 aacctaaatc acggataagc tgtaaatttc gatctatatg ttccgttgct tcagggtgga
    10741 atgtaatata atccgctccg gctttggcaa aatccggaat caaacgctca accggtttta
    10801 ccattaaatg cacatcaatc gggcattgaa tgccgtaatc acgtaaggct ttgcagatcg
    10861 ccggcccgaa ggtcaaattc ggcacgtagt ggttgtccat tacatcaaag tgaattaagt
    10921 ctgcaccggc ttttaacacc tctgccacat catcacccaa gcgagctaaa tcggcagata
    10981 aaatagacgg tgcaattaaa taaggattct tactcatatt tcctcactat caaaaaataa
    11041 atctacgtta gattaccata attttaacag cgaaaagagt ctctcttcct aaaaatccgt
    11101 ttttttacaa aacaccataa aaaagttatg gaattgtatt taaagttcgt atattatgta
    11161 tcccatattt taattatggt taatgacctt atctaaattc tggagaaaat atgaaaaaag
    11221 ggattcatcc tgaaaattat cgtgaagtgt tgttctatga tgcctcagtg caacaaggct
    11281 gggttatccg ttcttgtgcg gcaaccaaca aaacaatggt atgggaagat ggcaaagaat
    11341 acccacttta caccttagat acctcatcgg cgtctcaccc tgtttacaca ggtaaacgcc
    11401 gtgaagtgaa tacagaaggt cgtgcaagtc aatttaaaga tagatttaaa ggctttgctt
    11461 caacgctttc aaatcaaaaa tagaggataa aatgaaaatc ttaaactcac ttaaaaccgc
    11521 aaaatcacgc cacccggatt gccaaattgt cagacgcaaa ggcaaacttt atgtgatttg
    11581 caaaaccaac ccgagattta aagctcgtca gcgttaaaaa ccagcacctt aataagctaa
    11641 tttattaagg tgttttttat ttcatcttct caatattacc tattttaacc acatcaacga
    11701 ttttattatt tcttattcca tttagttatt tgcaagcggt caattttccc cttaaatttg
    11761 caaaaaattt attttaccaa ttaaaaggaa atgattatgt tgaaaaaatt atcaattgct
    11821 gcattagctc ttaccctttc tgtttctgca tttgctaaag agagcctact tgagcgtata
    11881 aacaacaaag ggactattac tgtgggtacg gaaggaactt atgcccctta cacctatcac
    11941 gatgcgagcg gtaaattaac cggttacgat gtagaagtaa cccgtgcggt ggctgaaaaa
    12001 ttagacgtaa aagtggaatt taaagagaca caatgggacg ctatgttagc cggtttaaaa
    12061 ggtgagcgtt ttgacttagt ggcaaaccaa gtggctttaa ccagcccgga acgtcgagcg
    12121 acatttgata aatccgaatc ttacagctgg gacggtgccg ccttattagt gcgtgaagat
    12181 gaaactaaaa tcaaaacccc tgctgatgta aaaggcgtga aaactgccca aaccttgagc
    12241 tcaaactatg gcgagattgc ccgtgaaaac ggtgcagaca tcgtgccaat tgacggtatg
    12301 gctcaaggct tactgttaat tcaacaaaaa cgcgccgatg caactttcaa tagccactta
    12361 tcgctattag attacttgaa aaagaacccg aattccggct taaaaattgt gtgggaaaaa
    12421 gaagaaaaag tgggtgcagg cttaattgcg aataaaggca atgacgaagc attagcgaaa
    12481 atcagcaaag caatcgtaga attacgtgaa gacggcacgt taaaaaaatt gggcgagcag
    12541 ttcttcggta aagatattag tgtacaataa tccatattgg attaggcttg gggcgaattc
    12601 gcccctttta tttttaaatc tccctcaaaa cgggagattt taggtttttc agatgttaaa
    12661 cgatctactt ctctcaatcc cctttatgac ccaagctcgg gtcgatttag tgctaagcgg
    12721 tttttggccg atggtacaag cggcattttt agtctctatt ccgctggcaa tcgcctcatt
    12781 tattatcggt atgttaattg ccgttgcagt tgcattagtg cgaattagcc ctgcaacagg
    12841 cttttttcac cgcttgctac tgtggctggt aaaaggttat atctcgatta ttcgcggcac
    12901 gccgatgttg gttcagatct gtattgtgtt ttatggtttg cctgcaatcg gcatttttat
    12961 cgatccgatt ccggcggcaa ttatcgggtt ttcactcaat atcggggctt atggctcgga
    13021 aacgatccgt gctgcgatct cctccgtgcc aaaaggacaa tgggaagccg gttatactat
    13081 cggtatgact tatatgcaaa cttttcgccg tatcattgcc ccgcaagcgt ttcgagttgc
    13141 tgttccgcca cttagcaaca cctttatcgg tctgtttaaa gatacctctc ttgcctccgt
    13201 tgttaccgta accgaaatgt tccgggttgc tcaacaggta gcaaatatgt cttatgactt
    13261 cctacctgtt tatattgaag ccggtttgat ttattggtgc ttctgctggg tgctattttt
    13321 aattcaggcg agattggaag tccgctttaa ccgatttgtg gcgaaataag gaggaattgt
    13381 gcttaaaatt cgcaatattc ataaaacctt tggcaacaac gctattctac gcggcattga
    13441 tttagatatt caaaaagggc aagtggtagt aattttaggc ccttccggtt caggcaaaac
    13501 cacctttctt cgttgcttaa atgcactgga aatgcccgag caaggcacat tggaatttag
    13561 tggtaatgct ccgctgaaaa ttgattttgc cgtaaaacag agcaaaaaag agattttagc
    13621 gctacgccgc aaatccggta tggtgttcca aaactacaac ctgttcccgc ataaaaccgc
    13681 attggaaaat gtgatggaag gaccggtatt tgtgcaacaa caagcggtcg aaattgctca
    13741 aaaaaatgca aaagccctgt tagaaaaagt cggactggcg gataaagcag aactctaccc
    13801 gtttcaactg tcaggcgggc aacagcaacg ggttggcatt gcacgagcat tagcaattca
    13861 gccggagctg atgttatttg atgaaccaac ttcggcactt gatcctgagt tagtgcaaga
    13921 cgtgctaaat acgatgaaag aactggcaaa tgaaggctgg acaatggtcg tggttactca
    13981 cgaaatcaaa tttgcccttg atgttgccga tgtagtggtg gtaatggacg gcggtgaaat
    14041 cgttgaacaa ggctcgccac agcaactgtt taaccaccca caacacgaac gaactaaacg
    14101 ttttttaaat caaatcagag cggataaata agaaacgata gcgctagcgt ccacgctagt
    14161 gctatctaat aaatggtaca agcggttaat tatgtataaa ttgttatact gttttggcac
    14221 gagcgtggac gctcgcgcca tcgtacagta aaatcagcta acaaggttcc ccataaagga
    14281 taaataagac aaggctcctt aatcttttag attaaggagc ttatttttag attttagaaa
    14341 tccgatttaa gccgcagccc atagtccacc aaaaatttgg tagaacaagc tatttagtag
    14401 caataagccg aaacctagca ccattaccga gaaatctaac attcccgttc ttggcaggag
    14461 cttgcggata aaacctaaca ccggttcggt aatttgtgct accgtgtagt ctaatgggtg
    14521 gttgccttga gtgacccagc tcattaatgc acgaatcaag gtggtgaaaa atagaatttg
    14581 tccgaaggtt ttgaccacac ttaataagcc gattaatact gctctgggta aatctacgcc
    14641 aaacagaata aactgtaacg ttaccaaagc ggcggcaatt aatagtgccg gaaagcaaat
    14701 atttttaacg gttggaatca gcttgccaac cggtgaaacc atcggttcgg taattttaag
    14761 taaggtttgt gaaatcggta aacgaggatc aacccgacaa aattgcagcc aagttcttag
    14821 caccagcaca aaacttaaaa agccaacaat aagcgaaata acaggagcta aaaattccat
    14881 ctctttttct ctttctatac aaaacaagcg gtcgtttttt agaaaaaatt tgcaaatttt
    14941 cagtaaaatc tgaccgcttg tatcttatta aattaactag gcaaattaag cttgtgctgg
    15001 gtagtcccac caaatatcaa ataattcgct cacttgaacc tcttttaagc cgttagtttc
    15061 taaccaagtt ttcactaatt ggcggtgaga ttcatcacat ttgcctaatt tttctaagca
    15121 gactaaacct tcccagttta agtagccgtt cgcttcaagg gctaaaccgt ttggtcgaat
    15181 cacttcatcg ataaaacgat ctacggtttc atcaatttgg tcaatgcctg tgccttcagc
    15241 aaaggtgaat ttaattaaga aacctaattc ttggtattca cctaagtgca tttttttacg
    15301 ttggcgtgcg ttacgttgaa tagccatttt ttctctcctc tatgaataaa tcatttcttt
    15361 gtaaaatata aatcccacac cccgtgtcct aaacgatgcc ctcgctcttc aaatttggtt
    15421 agcgggcgga aattcggacg tggaataaaa tcattagttg ctgatgtatt ttgcaactcg
    15481 ctctcaaatt ggcgtaatac ttcaagcata tgttctgcgt aattttccca gtcggtggca
    15541 aaatgaataa agccgttagg gcttaatttt ctgatcacac ggctgataaa ttcaggctgc
    15601 acaatacggc gtttgtggtg ttttgctttt tgccaagggt ctgggaagta aagctgtaag
    15661 ccacctaatg taccgtcagc aatgctgtct cgcaaaattt cggtggcatc gtggcaaatt
    15721 actcttaaat tcttcacgcc tttttctaac gcataagcga tacaagcccc caccccgggc
    15781 gtgtgcacct caatgccgat atagttgcga ttcgggtttt gctctgccat ttccaccagc
    15841 gattttccca ttccgaagcc gatttctaac actaccgggt tgttattgcc gaaaatctgc
    15901 tcaaaattaa agggcattgg ctggtaatct aagcctaaat ttgcccagtt gttgttcatc
    15961 atatcacgtt gataatcgct caaacgaccg gtacgcaata caaaactccg cacttttcgt
    16021 aaataacgcc catcttcggt aaactcggct tgctcaacgg ttttacgttt tttatctgca
    16081 aaagtctgtt tttctgacat tttcttgcct taaaacttca ctaataccga accccaagtc
    16141 cagccaccgc cgaatgcttc aaggagtagt aattgcccac gttgaattcg cccgtcacgc
    16201 accgcttcat ctaatgccac cggcactgtt gctccactgg tattggcata tttatcaagg
    16261 gtaaccacca cttgatccat ttccatctct aattttttcg ccgttgcggt gataatgcgt
    16321 aaattcgctt ggtgcggaat cagccaatct aaatcggatt tttgtagatt attcgcttct
    16381 aaggtttctt ctaccacgct tgaaagttgg tttactgcaa ttttaaaggt ggcattgccc
    16441 tgcatttcaa taaagccgga acgctcttcg ccacgctttt ggcttcttaa ggttaagtta
    16501 ttttctaaat ccggtgaggc gtgcaagtgg gttgaaataa tacccagctc ttcactcgcc
    16561 tctaaaatca ccgccccagc tccgtcacca aataatacta ctgtgctgcg gtcggtttcg
    16621 tctaacattc tggaattgat gtctgaacca ataaccaatg cctttttcac ttgcccgtta
    16681 cgcacgaatt gatcggcaac agttaaagca tacacaaatc ctgtacaagc cgctgccaca
    16741 tcaaaagcaa tcgcgtcttc aatctgaagc aagccttgaa tttggcaagc tgcacttggg
    16801 taagcgtggg agttggtagt tgttcccacc acaattaaat caatgtcatt tgcctcaatt
    16861 cctgccattt ccagtgcttg ttgagcggct tttgccccca ttgttgccac tgtttcgtca
    16921 ggctcggcaa tacggcgttc acgaatgccg gaacgggtta cgatccattc atcagaggta
    16981 tcgaccattt tttctaaatc agcattggta cgcacctttg tcggtaaata gcttcccgtt
    17041 gctaaaattc tactattcat atttttatta ttctcaatca attaaatgat aatccgttat
    17101 tatagcttga aaaccaacaa atatataggc tttacaagcg gtcattttta gcaaaaaagt
    17161 tgcattcacg aacgattttg cactagaacc accgcttgct agtatttatt tcgcttgagc
    17221 ctcttcgtat agccacttcg ctactcgctt ggcgaaataa gttagaatac cgtctgctcc
    17281 tgcccgttta aagcagagta atccctccat aatgcactca cgctctttca gccagccgtt
    17341 ttggattgcc gccatatgca tcgcgtattc gcccgaaact tggtaggcga aggttggcac
    17401 gccaaaattc tctttcacac gccacaccat atccaaataa ggcatacccg gtttcaccat
    17461 caccatatct gccccttctt gaatatccat cgccacttcg tgcaagcctt cgttgccgtt
    17521 agccggatcg acttggtaag tgtatttatt accgcctttt aagttgccgg cagaacctac
    17581 tgcatcacgg aacggtccgt aataattaga cgcatattta gccgaatacg ccataatttg
    17641 cgtattgata aaaccgtttt gctcaagggc ttcacgaatt ttgccgattc gaccgtccat
    17701 catatcgctc ggggcaacaa tatccgcccc cgcttctgcg tgcgataacg cctgttttac
    17761 caatacttca gtagtcacat cattcagtac atagcctgtt tcatcaataa tgccgtcttg
    17821 tccgtgcgtg gtaaacgggt ctaacgccac atcagtaatc acgcctaatt ccggaaattg
    17881 agcttttaaa gctcgcacgg cacgctgtgc caacccttgc gggttatagg cctcttctgc
    17941 catcagtgat tttttcacat cacccaccac agggaaaaga gccaccgcag gcacgccata
    18001 tttaaccagt tctgcggctt caactaaaag ctggtcgata gttaaacgct ctacacccgg
    18061 catagaaggc acttgtactc gctgattttc cccttcgatg acaaaaacag ggtaaatcaa
    18121 atcattaacg gtaagttgat tttctgccac taagcggcgg ctgaaatcgt gcttgcgtaa
    18181 acgacgcata cggcgtaaag gatattggga ttgaattact tgcattttta atttcctaat
    18241 tcttaaaata cctattcttg aaataagaaa ggcatagcaa acaggtgcta tgccttttca
    18301 aaatattact gtgcaacctg ctctgatggt tcatcatcat ctttggggcg ataaaaccga
    18361 gcaaatagca caccaacttc aaacaataaa cacattggaa ttgccaataa tgtttgcgag
    18421 aaaatatctg gtggggtgag caacatacca attacaaatg ctgccacaat aatataaggg
    18481 cgttttgcct ttaaatcttc gggtgtggtt acgcctgccc aacataacaa gataatcgca
    18541 accggcactt caaagcagat cccgaatgcc ataaaaatgg ttaaaacgaa atcaagatag
    18601 ctactaatat ccgttgccat tgttacccct tccggagcgg tagaggttaa aaagccaaat
    18661 acaagcggaa atacaacata ataggcaaaa gcgacaccac aataaaacag caatgtgcta
    18721 gataccaata atgggtaaac caaacgcttt tcctgcttgt ataaagcagg ggcgacaaac
    18781 gcccaaattt ggtacaaaat aaaaggaact gataaaaaca ccgaaaccac aatggttaat
    18841 ttaattgggg taaaaaacgg agtggctaca ttggttgcaa tcattgttgc accgtctggt
    18901 aaattgtccg ttaaaggtgt tgccaataag gtgtaaatat cattggccca gtaaaccaat
    18961 ccgcaaaaca ccaagagcac acagataaaa ctacgcagta atcggtttct cagctcaatt
    19021 aaatgtgtaa tcagaggttg agactcatca acttgcgaca ttatttgcct ccgatttctg
    19081 cgctgattct gatgattgag gcgtagctaa atcatcatct ggcggatagt aagattccaa
    19141 cgttaaaagc gattcgtcca tttctgcttg ttcagccagc tcatcagccg aaagtggctt
    19201 ttctgtccgc gtaacattaa gttgctcaga taaactatcc acttcaagcg gtttattttg
    19261 cgcatttttt tgcaattctt gcgcctctaa cacctggctt tcttgctcga ttttttgctg
    19321 aatttctgcg acttgctcat cggttaattt aggaattgtg ctgctttccg cagccgtttt
    19381 ttccaactca ttttttgcag aattaagcga atcttgcaac tgggtggctg acattttcag
    19441 atcttccacc gttttattta attctggcga aagggagctt aaattcaact cttctgcctt
    19501 tttaatgctc tcttttaatt cttgcagttt cagctcttga gccagttcat tttgtacgtt
    19561 tgcggctaaa cctcgaattg tactcaccca gcccattacg gtacgaatcg caatcggcat
    19621 acgtttcggg cctagcacca ccaagcccac aatcataatc agtactaatt cagaaaaacc
    19681 tatatcaaac acggattaag cctgctcttt ctcttttgct ttctgctctg ctgaaattgc
    19741 atcttttgct tccacttttt caaattcagc atcttttttc tcatctgcca ttgctttttt
    19801 gaagcctttt acagattcgc ccaaatccga acctaaagta cgtaatttct tcgtaccgaa
    19861 aagtaatacg atgattgcaa ccacaatcag aagctgccaa atactaatac cgcccataaa
    19921 aactcctttc atttaatagg gaatagataa aataaaacgc tgtttattat atgttacttt
    19981 gacatataaa aaagtaattt tgcaaaaaat ttgtcaaaaa caaccgctta tttgcgttga
    20041 ctcaaaatca ataccagtgg aaataaagtt aataacggca cacttagcca tatcggcaaa
    20101 gattcaaatt tccaaaatgc gccacagatc acaatcgctg aaattgacag cacaagaaaa
    20161 cggctatttg cgtttgaatt ctgtgctttt aaactttcgt taatttccgc taaacggaaa
    20221 ttaatttgct tttgctcttg taaggcttta aacaatgcct ccggaaactc cgcaaaatgc
    20281 tcccgatatt gcggtaaacg ttgcttaatg tctcgaacca tcgctttcac acctacctgc
    20341 tcattcagcc agttttgtaa aaacggtttt gcggtttgcc ataaatccaa ctgtggataa
    20401 acctgcctgc ccaaaccttc aatataaaga agtgtttttt gcaacaacac caactgcggc
    20461 tgcacttcca tattaaattc tcttgccaca ttaaacaggt tgagcaaaac gtgtccgaat
    20521 gaaatttccg ataacggctt agcaaaaatc ggctcacata cctcacgaaa ggcttgctca
    20581 aatttatcaa tatcggtatc cggcggcgtc cagcccgatt ccacgtgcat taaggcaaca
    20641 cggcggtaat ctcggttgaa aaatgcgaca aaactttcgg ctaaatagcg tttgtcattg
    20701 tggttcaacg tgccgacaat cccgcagtca atgccgatat attgcggatt ttccgggtgt
    20761 tctctgttca caaaaatatt gccggcgtgc atatcggcgt ggaaaaaact gtcacggaat
    20821 acttgggtaa aaaatacctg cacaccacgc tctgccagca atttcatatc ggtgctgttc
    20881 gcttccaact cggcaatgtt agacactgga atgccataaa tccgctccat cgcaatcaag
    20941 tttttatggc aaaaatcttg atacatttca ggcacataca gcatttcgct attttcaaaa
    21001 ttcgcccgaa gccgaatggc attgtgcatt tctttgcgta aatccagctc gtctaaaata
    21061 gttttttcgt actctcgcac cacttcaacc gcccgtagcc gtttgccgtc attcgataat
    21121 ttcgggataa agtgagcaat ttcatacatc aactctaaat ccgccttaat caccggctca
    21181 atgcccgggc gaatcacttt taacaccaca tctttgcctg caagcggttg attttggtta
    21241 aattttgccg tgtgaacctg tgcaatcgaa gcagaagcta aggcagtttc atcaaagttt
    21301 tcaaaccaag tttcaagctt tccgcccaaa gcctgctcga taatttgacg agcgattttg
    21361 ccgtcaaacg gttcaacatt atcttgtagc aaggcgagct ggtcggcaag ctccggtgca
    21421 aacagatcac gtctggtagc taacatttgc ccgagcttga tccaaaccgg tccaagctct
    21481 tgcaaagcca aacgtaaacg tacaccgtaa ggtttgtctg aatgttgatt cttcacccaa
    21541 aacaaagcct tacgccctag ctttattttg cgggtaattg gcagattcgg aatggcttca
    21601 tcaataccat agcgaagaaa agtgcggata atatggtaaa agcggttgag atttttgatg
    21661 ttcattgaaa tatatattta atggtaatta ggaagatttt agcaaaattc tgtaaaaatt
    21721 taaccgcttg ttgggctact atgggtattt ctgctcaaaa ttctcaattt ctttctgtac
    21781 ttccgcaata agtgtttgtt cagaggggag gtaaagttgg tattggctgg caaaaatttt
    21841 attctgattt tccggtagcg aaaattttac caccgcatca tttttatcgg cacaaagcaa
    21901 gacaccaata gtggcatttt cgtgcggcag tttttcaact cggtcaaaat aattgacgta
    21961 catctgtaat tgccccaaat cttgatgggt gagcttgtgg gttttaattt caaaaatcac
    22021 aaaacattga agtaaacggt tgtaacaaac caaatcgaca aaaaaatcat caccgtctag
    22081 gtgcaaacgc ttttggcgtg cgataaacga gaaaccgtta cccaattcta gtagaaaatc
    22141 ctgcaaatgc gtaatgatgg cttgctctaa atccttttcg taataagcac tttcccgttt
    22201 tagtccgaga aattccaata ccatcgggtc tttaatgagc tgtttcggat caaccggctg
    22261 ctgctcattt ctggcaacag caagcacact ttccttatca ttactgagca ataatcgctc
    22321 gtatagacta ctattaattt gccgttccag ctggcgtgaa ctccaattat tcttacaact
    22381 ttcagccaaa taaaattccc ttttgtcaga atcggagatt tgaattagac gacgatattg
    22441 tgtccaattc aattgcgacc gcagtgcggt cgcaattgga aacgcacgat aaaactggcg
    22501 ataacgctct aattgacggc tacaaaaacc acttccaaat tctggctcaa gctcttttgc
    22561 gagcatttta atcaggtatg ttccataatc agcacgctct tgcccttgtt gctcctgttc
    22621 aaaaatccgt tttccgatgt gccaatacat cagcacacgt tggaaatcta ccgcacgaac
    22681 cgcattttca cgagcttgag caataatttg tttaatatct tagatcatcg ttgtttctat
    22741 taacataaaa gcctccgttt tggattaagt ttatgcttaa tctatccgga ggcaatttct
    22801 ttcactcaac ttttcgagtc atatcacaat tttattttgt taaatttaat tttgcacaaa
    22861 cgcttgcgtt tgaacatcag ctttgctgat ggctcgaaga gcgaacgtag tgagtaaatt
    22921 tttgctctaa tttgagcgct tgttgggcaa gttcttcgac ttggtcatag aaatgcaccg
    22981 cctgtaaacg atgcaccaac accggtcttt cttggattaa attctcaacc aaatgttgat
    23041 tattttgctc aaattgagct tttactttac ctagcaattt tttagcaaga tcggtgctag
    23101 tttgagccac tacatcgcca acaaaagggg aaagcagttc ggcagggtct ttttcgagct
    23161 gttcaagtaa tgcagtgaaa tgttgcaaca cttgaatatc gcccgtcagc accaacgatt
    23221 tattattaat cagctcggtt aatttttgct tatccgctag tttcggcagg gtgtcaaatg
    23281 ccagttgcac cgtacaatct gcttccccct cgtagttgcc aagccaatcg gttcggcttt
    23341 cgctaaacaa cacaaaaaag tcgattttag gcgaagttag ggcgattttt agcacttttc
    23401 cggacaattt acgcaaattc ggctcaatat gaggcgagcg tttaagtaac gcattaaaag
    23461 cggtttcaat cagaccaaag gtaaattgcg ggagcattaa ttgttgtttt acattattta
    23521 acattcgtac ctacttaccc tcttcacttt tgggagaagg atagattgtt cttcattaag
    23581 aagcaaaatc agggagaggg gtatttgcaa aatattgaat aaatatgacc gcttgccccc
    23641 tctctctgct tatttcgcta aacgctcata agcggtctct ctcccacaaa gggagagagt
    23701 catttaagag aaaactaaaa tttataacct ctgtgtaatg ccacaatgcc ggcacttaag
    23761 ttgtagtaat tcacgctttc aaagccagtt tgttccatca ttgcttttag ctcgtcttgt
    23821 ttcgggtgca ttcggatcga ttcagctaaa tagcggtaac tttcggcgtc atttaccacc
    23881 acttcgccca ctttcggcaa aatattgaat gagtagaaat tataaagctg gctgattggg
    23941 tcgataatcg gttttgagaa ttccaacaca agtaagcgac cgcccggttt tagcacgcgg
    24001 aacattgaac gtagggcttt gtctttgtcg gtaacattac gcaagccaaa actaatcaca
    24061 atacaatcaa aagtgttatc agcaaaaggc aagcattcgg cattcgcttg aacgtagctc
    24121 acatttccaa ccacgcctaa attgcgtaat ttttcccgcc cgacttccaa cattgagctg
    24181 ttaatatcgg ctaaaatcac ctcaccactt tcaccaacaa tgcgagagaa ttttgcggta
    24241 aaatcacccg tgccgccggc aagatccagc actttttgcc ctttacgcac gccactgcaa
    24301 tcaatggtaa agcgtttcca cactcgatga atgccgaaag aaagcaaatc gttcattagg
    24361 tcatatttgc ctgccaccga gtgaaacaca tttgcgacca gttgctgttt ttcttctttc
    24421 gctacggttt taaacccaaa gtgggtggtt tctgccgatt gttgcacttc ttgttgcact
    24481 tcatctttag gtgctaattg ttctacatcg ctcattttct tgtctctagt tgcaaaattt
    24541 aggcgtaacc atagcaaaaa agcggtcgtt tttctttaac tttttgcaaa ttttcttaag
    24601 taaataaccg ctttcttgac tagaaaagcc aaattctgta acattcgcac actatttttt
    24661 cttgaggatt acacattttg gacagtcata gtcgatactt acccgtttaa cctatcttgt
    24721 ctaacctcaa tgttaccgac aaggtcggta atgctcgcct agtatctttg ttcctttttg
    24781 ttccaagcct cctaactatt tggagctaaa catgatccgt gcttttggac tagataacgc
    24841 atgtttaatc agcgttgatg aaactaaccc aactttatta aatgatgcga tttggattga
    24901 tctcatcgat ccatcagaag ctgaacgaac cgtattagaa aaccgcttag accaaacact
    24961 cgctgaagaa cgtgaattgg aagacttaga ggcgtctgcc cgtttctttg aagatgaaga
    25021 cggtttgcac ctgcactcgt tcttttactg cttagatgat gacgattacg ccgatattgc
    25081 cactgtcgcc ttcactattc gtgacgggcg tttgtttacc ttgcgtgatc gggatttacc
    25141 cgcatttcgc ttgtatcgaa tgcgttcccg ccgtgagaaa ttgattgaca gcaacgccta
    25201 tgaactctta ctcgatttat ttgagaccaa aatcgagcag cttgcagacg taattgaaga
    25261 agtctatgct gacttagaaa aattaagtcg ggttattcta aacggtcagc aagaaaatga
    25321 gaatttcaat gatgcccttt ctcgcttaac tgaatttgaa gatgcaagtt caaaagtgcg
    25381 tttatgtttg atggatacgc aacgggcgtt aagtttctta ctgcgtaaaa cccgtttgcc
    25441 gaataatcag ctagaacaag cccgtgacat tatgcgagat attgaatctc tgcaaccgca
    25501 caatgaatcc ttgttccaaa aagtgaattt tttaatgcaa gcggcaatgg gttatatcaa
    25561 tattgagcag aaccgaatta tgaaattctt ctcggtggtg tcagtgatgt tcctacctgc
    25621 tacgctggtt gcttctactt acggtatgaa ctttgaattt atgccggaac ttcatttcca
    25681 atatggctac cctatggcga ttggtttaat gattgttgcc gcctccgccc cttatcttta
    25741 ttttaaacgt aaaggttggt tataattta
//
LOCUS       DANLUQ010000016        25630 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.016, whole genome shotgun sequence.
ACCESSION   DANLUQ010000016 DANLUQ010000000
VERSION     DANLUQ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 25630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25630
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.016"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            26..1942
                     /gene="iolC"
                     /locus_tag="PXB27_000560"
     CDS             26..1942
                     /gene="iolC"
                     /locus_tag="PXB27_000560"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HDL5345383.1"
                     /translation="MATVNHQDRPLDLICLGRVAVDFYGQQIGSRLEEMTTFSKYLGG
                     SSGNVAYGTAVQGLRSSMLARVGDEHMGRFLREELQSVGVDTSHLITDKERLTALVIL
                     GIKDKETFPLIFYRDNCADMAITKDDFSEAYIASSRCLAITGTHLSNPKTREAVLTAL
                     EYAGRNEVKRALDIDYRPVLWGLTSLGDGETRYIDSAEVTKSLQEVLHHFDLIVGTEE
                     EFHIAGGSTDTLTALKNVRKVSNAVLVCKRGALGCSVFEGELPNDLDGGLNVYGVRVE
                     VLNVLGAGDAFMSGLLRGYLNNEGWEQACHYANACGALVVSRHGCSPAMPTKRELDDY
                     LTRAESVPRPDLDERLNHLHRVTTRKKQWENLCIFAFDHRKQLVDMANKVGADLARIP
                     KLKQLLLKAMEQTATQSGLPQNQAGILADTTFGQDVLNEITGKDYWIARPIEEPSSRP
                     LELEYGDLGSQLASFPRDHVIKCLAFYHPNDESGLKARQDRKLKEVYQACCRTGHELL
                     LEIILPADMPQSEAFYNQAIAHFYQLGIKPEWWKLPDVSRAQWQAISATIEANDPDCR
                     GILILGLDAPLEVFKDTFEQAKGCEKVKGFAVGRTIFGEPSEKWLAGELDDNTLISAV
                     SEKYRTLIDLWQQR"
     gene            2232..3572
                     /locus_tag="PXB27_000561"
     CDS             2232..3572
                     /locus_tag="PXB27_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HDL5345384.1"
                     /translation="MSCNTSPKRFHASWESIKQHQVPEWYEDAKFGIFIHWGIYSVPA
                     FAPPTCQLGEIDIDEQWFCNNPYAEWYFNSINVKKGPTYEHHIKTYGESFAYNDFLPL
                     WKAEKWQPAEWAKLFAKAGAKYVVLTTKHHDGFCLYPSKYTDFNCMQAGPKRDLMGEL
                     TENVREQGLKMGAYYSGIIDWTYSSAPIFTESQNFSNACPTYEYADYAYKQVVELIDR
                     YKPDVLWNDIGWPKVGEHMLPHLFAHYYNSVQEGVVDDRWNKLWCDFTSKEYKHGVAS
                     RDKKWEMCRGMGLSFGYNQVEDESHLISVKDLISLLVETVADNGNLLLNIGPKADGTI
                     PQEQVERLLALGSWLEINGEGIYKSRCSQHQTEQIGDVTYRYTKVGADLYLYIDGLTE
                     AQISLPLSVYGELQALNTELKFTIENTAQGRVLHIQNYQPDWYVLGFKIADGEK"
     gene            3575..4915
                     /locus_tag="PXB27_000562"
     CDS             3575..4915
                     /locus_tag="PXB27_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL5345385.1"
                     /translation="MSVSQRFIDFMNAHVAPVARRMENQPHISAIRDGFIVVLPFLIV
                     GSFIMILLIPPFDENTQNAFGQAWWRFANWASPYGWNFFQMSFNAISLFTSASIAYNL
                     AKAYKREPLPAAFLSVMAFLLVAAPVKDGMMDIKFFGGIGLFSAIFIAIYSVEMTRLL
                     EFLKIKIRLPKEVPHAVAESLNIVIPILAILLTLYPFAIWVESATGRNIPQLIMDFMA
                     PLIAVSDSLGAICLFVIATHLLWFLGINGSLVLMQLWTPFLLQNMAANLAAFQAGEPL
                     PFIITNSFWDFYIVHGASGGVIALAFLLVRSKSAHLRSIGKIGLVPSFFSIGEPIVYG
                     VPMVVNPLFFIPLIFAPLANAVIAYLILDFNLIHRVYLMAPWTTPAPIGAYLVSAGDI
                     WAPILSIALIILDIVIYYPFFKMYEKVCVEKEKAQNLDEKAQQELLEKARMAAQ"
     gene            5053..5877
                     /locus_tag="PXB27_000563"
     CDS             5053..5877
                     /locus_tag="PXB27_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL5345386.1"
                     /translation="MNGPTQLAHFQEQIRKEYDGLSKRLKQVAQYVLDNGNSVVFDTV
                     STIAERADVPPSTLIRFANAFGFSGFNEIKQLYRQDMMEGTSRYTERVQLFRQIEPLD
                     EQNSELNHILDVFVQGNTQALQQLSTQVSEEQLAQTVDILHNAKRIFIVGLKRSFSIA
                     NYLNYALHHLDYDVFMIDGSGGMFDEQLSRIREGDAVIAVSFSPYANETLNVVTATAR
                     PGVKHIAITDSQLSPLLSFSDVSFIIKEAQVSGFRSQCATMTLAQTIAVSLALKKA"
     gene            complement(5921..6163)
                     /locus_tag="PXB27_000564"
     CDS             complement(5921..6163)
                     /locus_tag="PXB27_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL5345387.1"
                     /translation="MSDKSIILEIINALGNKENIKQVEACLTRLRVVLHNNGLLNKSA
                     LKKLGAVDVIKVGDTQQIIFGAKAGQYRDEINALIG"
     gene            6428..6676
                     /gene="rpsP"
                     /locus_tag="PXB27_000565"
     CDS             6428..6676
                     /gene="rpsP"
                     /locus_tag="PXB27_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDL5345388.1"
                     /translation="MVTIRLTRGGAKKRPFYQIVVVDSRSPRDGRFIERIGFFNPLAA
                     GQAERLRLDVAKVDAWVAKGADLSDRVASLVKEARKAA"
     gene            6709..7236
                     /gene="rimM"
                     /locus_tag="PXB27_000566"
     CDS             6709..7236
                     /gene="rimM"
                     /locus_tag="PXB27_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDL5345389.1"
                     /translation="MSEQKIEVVGKLGSTYGIRGWLRLYSSTEQTESIFDYQPWFLKI
                     KGQWQQVELESWRYHSNDLIVKLKGTEDREAAQLLTNAEIGVDLSVFPELEEGDYYWH
                     DLIGCSVVNLEGYTMGTVAEMMETGSNDVLVVRNNGKDAFGKQERLIPFLYEQVVKRV
                     DLATKTITVEWDAGF"
     gene            7281..8036
                     /gene="trmD"
                     /locus_tag="PXB27_000567"
     CDS             7281..8036
                     /gene="trmD"
                     /locus_tag="PXB27_000567"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDL5345390.1"
                     /translation="MWIGIISLFPEMFKAITDFGVTGRAVKQNLLQVECWNPRDFTFD
                     KHKTVDDRPYGGGPGMLMMVQPLRDAIHAAKKAALAVEGGEAKVIYLSPQGRKLDQNG
                     VQTLASNQKLILICGRYEGVDERLIQTEVDEEWSIGDYVLTGGELPAMTLIDAVARFV
                     PGVLGKQASALEDSFAEGLLDCPHYTRPEVLDGMPVPEVLMSGHHEQIRKWRLEQSLE
                     RTWLRRPELLDSLALTDEQRVLLAKIKKQHKIS"
     gene            8063..8413
                     /gene="rplS"
                     /locus_tag="PXB27_000568"
     CDS             8063..8413
                     /gene="rplS"
                     /locus_tag="PXB27_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDL5345391.1"
                     /translation="MSNIIKQLEQEQLKQDLPSFRPGDTLEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTLRKVSNGVGVERVFQTHSPVIDSISVKRKGAVRKAKLYYLRE
                     RSGKSARIKERLGD"
     gene            8569..8925
                     /locus_tag="PXB27_000569"
     CDS             8569..8925
                     /locus_tag="PXB27_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345392.1"
                     /translation="MKQLSIRLPDDEFTFIKTFASAHKLTLQNFVMSAIQAKVAEMSA
                     KNKISEESNIHQPLSKDEKIMLNEAKVFYSARSHLKAENVILPSDFMEQIKSGCGAEK
                     MAEMMGYTKEDWESMK"
     gene            8927..9250
                     /locus_tag="PXB27_000570"
     CDS             8927..9250
                     /locus_tag="PXB27_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345393.1"
                     /translation="MGQITFSNIFTKVIFENVLSASTKEKIKQMLFQSVVALPPLHSE
                     RKMILQLKKQKITIFYQVIEEQSVQILAYQFGGTDKLTGVSKAHFKTLDAIFQMTDEE
                     KEGKF"
     gene            complement(9260..9592)
                     /locus_tag="PXB27_000571"
     CDS             complement(9260..9592)
                     /locus_tag="PXB27_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDL5345394.1"
                     /translation="MQIEQYQQWVRQFYQQQGWYERSPFMRVTYLAEEIGEVACAVRA
                     IEIGRERPDETEASQQQKRDNLIEELGDVMDNLFVLADKYGISMTEVLERHQAKFEKR
                     YLQKNMEK"
     gene            complement(9685..10881)
                     /locus_tag="PXB27_000572"
     CDS             complement(9685..10881)
                     /locus_tag="PXB27_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDL5345395.1"
                     /translation="MNIPFCLPENISPETFLREYWQKRPLLIRNGLPQIVGLFEPEDI
                     LELALEEEVTARLIQCENEKWSVKSSPLVEEDLEELPVQWSVMVQNLEQWSPELGELW
                     QAFGFIPQWQRDDIMVSCSPLNGTVGKHYDEYDVFLVQGYGQRRWQLGKWCDPSTEFK
                     PNQPIRIFDDMGELVMDEVMNPGDVLYVPSRMAHYGVSESEQGLTFSFGLRYPNATDL
                     LENLCKTIEQQADNINSSEFHLPFRLTPNEQPNALLDPKMVKMFKHQLIDLLQNSEQF
                     DELLTHSVATAASSRRYELLQTDNEYYPDEVQEILEAGGWLQQDANVKILYTENPQRV
                     YVNGEWIDELNEAEMALLIRIANGDSISWNKLASKVQDQAELELMLDTICDWLDSGWI
                     ILSEAE"
     gene            complement(11030..12373)
                     /gene="accC"
                     /locus_tag="PXB27_000573"
     CDS             complement(11030..12373)
                     /gene="accC"
                     /locus_tag="PXB27_000573"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDL5345396.1"
                     /translation="MLEKVVIANRGEIALRILRACKELGIKTVAVHSTADRELKHVKL
                     ADETICIGPAPSVKSYLNIPMIIAAAEVTNADAIHPGYGFLSENANFAEQVEKSGFIF
                     IGPTADVIRLMGDKVSAINAMKKAGVPCVPGSDGPVGNDPKKNKEIAKRIGYPVIIKA
                     SGGGGGRGMRVVHHEKDLEESIAMTKAEAKAAFNNDMVYMEKYLENPRHIEIQVLADT
                     HGNAVYLAERDCSMQRRHQKVVEEAPAQGITPELRQFIGERCANACREIGYRGAGTFE
                     FLYENGEFYFIEMNTRLQVEHPVTEMITGVDLVKEQLRVAAGLPLSITQDDIKVKGHA
                     IECRINAEDPNTFLPSPGKITRLHVPGGLGVRWDSHIYSGYSVPPHYDSMIGKLITFA
                     ENRDIAIRRMENALSETIVEGIKTNIPLHTQILADKEFRKGGTNIHYLEKKLGLK"
     gene            complement(12445..12912)
                     /locus_tag="PXB27_000574"
     CDS             complement(12445..12912)
                     /locus_tag="PXB27_000574"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDL5345397.1"
                     /translation="MDIRKIKKLIELVEESGITELEVSEEEGTVRISRAAPAVAPAAV
                     QYAAAPVVAPAAPQVASTVPVAAPAEAPKAAETSGHAVLSPMVGTFYRSPSPDAKPFV
                     EVGQTVKIGDTLCIVEAMKMMNRIESDKAGVVKEILVNDGEPVEFDQKLFIIE"
     gene            complement(13165..13947)
                     /locus_tag="PXB27_000575"
     CDS             complement(13165..13947)
                     /locus_tag="PXB27_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL5345398.1"
                     /translation="MAVKNEQFIVICNGVFRLFGNIVKILSYPFHALFPKKRFTIPEY
                     SPAKIQSSIPSKITKTIWQTNYSNKVTLPMYANYLFNRLMSLSYDYRYVSTEEREAYI
                     KANADERTFNAYSKLTDGAAQADFWRIFTLLREGGIYIDIDGHLVYPLSKIIGENDSE
                     VLIKRRDKYTNFFLASEAGNWILKDTLELIISNIENRRIEGGVFVMTGPDTLNNAIGD
                     KEVNFRRDKVTCAQGTFTNEYFQYIDKPGSKWNYKKNEDLLK"
     gene            complement(13957..14394)
                     /gene="aroQ"
                     /locus_tag="PXB27_000576"
     CDS             complement(13957..14394)
                     /gene="aroQ"
                     /locus_tag="PXB27_000576"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HDL5345399.1"
                     /translation="MKKILLLNGPNLNMLGKREPQIYGSQTLADIENHLKKLAAAQDV
                     ELESFQANGEEPIINRIHQAFQHTDFIIINPGAFTHTSVAIRDALLSVSIPFVEVHLS
                     NVHSREPFRHHSYLSDIAKGVICGLGAKGYEYALDFAISHLKA"
     gene            complement(14480..14848)
                     /locus_tag="PXB27_000577"
     CDS             complement(14480..14848)
                     /locus_tag="PXB27_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL5345400.1"
                     /translation="MAFTIRKMVEQDTHQVFPLMQKLAVFEHYIDSFAITPEVVMESG
                     FRKSPPDFYCLVAENASNIIGILVYYFLPYTAQNRPAIYMKELYVDETARNQKVGEAL
                     MQALKNEAKAHHCKTIKSLE"
     gene            complement(14908..15732)
                     /gene="dapD"
                     /locus_tag="PXB27_000578"
     CDS             complement(14908..15732)
                     /gene="dapD"
                     /locus_tag="PXB27_000578"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HDL5345401.1"
                     /translation="MSLQAIIEAAFERRAEITPKTVAAETKAAVEEVIAGLDNGSLRV
                     AEKKDGEWVVNQWVKKAVLLSFRIADNEIIDGAETKFYDKVPTKYGNYTEEQFKADGI
                     RAVPGAVVRKGSHIEKNVVLMPSFVNIGAYVGEGTMVDTWVTVGSCAQIGKNVHLSGG
                     VGIGGVLEPLQANPTIIGDNCFIGARSEIVEGVIVEDNCVISMGVFIGQSTKIYDRET
                     GEVHYGRVPAGSVVVSGSLPSKDGSHNLYCAVIVKKVDEKTLSKVGLNDLLRSIDE"
     gene            15880..16542
                     /locus_tag="PXB27_000579"
     CDS             15880..16542
                     /locus_tag="PXB27_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDL5345402.1"
                     /translation="MENFANLLTGKTREHLVPLPNALSDKHYLQPQVVEAFLTLQHAA
                     KQAGFNLQPASTFRDFERQKGIWNAKFRGERKVHDDNGVAIDMAALSDLEKCKAMLRW
                     SAVPGASRHHWGTEIDIFDPDLLPENTPLLLEPWEYLEGGYFADLTKWLHVNAETFGF
                     YFPFDGKHSRVGFEPWHISYRPISNEYEQRFNDEILKAAWQDEEVAGKECLLEHFEEL
                     LK"
     gene            16539..17291
                     /locus_tag="PXB27_000580"
     CDS             16539..17291
                     /locus_tag="PXB27_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL5345403.1"
                     /translation="MIQLINETQQHDKFRQICDKWQLVEDKSSPLALVLTEERLELRK
                     LDEPKLGAIAVNFVDGTMAHRRKFGGGRGEAVAKAVGIKGDYLPTVIDATAGLGRDAF
                     VLAAVGCKVLLVERNPIVAALLEDGLNRAYQDVEIGSFMQERMILADVRDISQLDPQS
                     QMAEVVYLDPMYPHKQKSALVKKEMRVFQHLVGADLDSDSFFLPAKGLAKKRVVVKRP
                     DYAPFLAEQKPDFSHTTKNHRFDVYLSHLKND"
     gene            17382..18218
                     /gene="ftsN"
                     /locus_tag="PXB27_000581"
     CDS             17382..18218
                     /gene="ftsN"
                     /locus_tag="PXB27_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HDL5345404.1"
                     /translation="MPQRDYVARSNEKKSKKNQKESNVKKILMRIIGVILFFGIGLWF
                     LKSSAPVPTVPSAPQPANSQTKSELPSRPEETWSYIRDLETRTVITDNSQTSQERLAQ
                     LTAEQKQQIEERRKQEEARLAAEQAQQKPATDVSATELAPKTEEVKSLTEEELAAKKA
                     QEQALAQKQAEEKRRQAELKKQQEAKKEQPAKIVASAATLADKAANQAGKFGLQCGAF
                     KNKAQAENMQARLAMAGFNARIASSADWNRVFVGPIGDRAATSRAQSNARSVAECVIV
                     AM"
     gene            complement(18260..19441)
                     /locus_tag="PXB27_000582"
     CDS             complement(18260..19441)
                     /locus_tag="PXB27_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HDL5345405.1"
                     /translation="MKTELDMIVIGGGMIGAATALGLAKQGLRLALLEKNPLPTFNPD
                     SDYDLRISAISMGSIQLLEKLGVWEQIVAMRSTPYTQLETWEIDGFNTLFSAADLGLE
                     KLGDMVENNVIQIALWEQLKQYPNCLQAVGFEQIFANKAGEYWEVTVDGNTFAAPLLI
                     AADGANSQVRQWAGIGLTAWQYRQHCLLATVTTEKPAPSGTWQQFYPTGPRAFLPLSG
                     NHGCVVWYDSPQKIAELKMLPAEKLGVAIEQHFPSRLNAELGNIQVQASGAFPLTRQH
                     AQHYVQNGVVLVGDAAHTINPLAGQGVNLGFKDVKTLLEVIEQAVKKGKNFASEQVLQ
                     RYEQKRKPDNLLMQTGMDVFYKAFKTDLLPLKIARNVGLVLADKVSPIKKQALKYAIG
                     I"
     gene            19548..19886
                     /locus_tag="PXB27_000583"
     CDS             19548..19886
                     /locus_tag="PXB27_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345406.1"
                     /translation="MSCKTHANHEHEHGEGCGHTAIKHNDHIDYLHDGHLHHNKHGDH
                     YDEHVLEVNETNPADCNAAHCCGDHVHGEVCGHEAVPHGDHIDYIVNGRLHHQHGDHC
                     DDHGPVEIVK"
     gene            20018..20887
                     /locus_tag="PXB27_000584"
     CDS             20018..20887
                     /locus_tag="PXB27_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HDL5345407.1"
                     /translation="MTLAIVYSRASIGVEAPLVTIEVHISNGQPKLTIVGLPEATVKE
                     AGDRVRSALLNANFIYPPQRITINLAPADLPKEGGRFDLPIAIGILAASGQIDSDLLT
                     QFEFLGELALTGHLRGVHGTIPAVISADKAKRQMILAKQNANEASLVSNATTYFAGSL
                     LEVVNMLNKRDKLPICQHISQHSAEIRPLVSRDLTDIIGQQHAKRALMIAAAGQHNLL
                     FLGPPGTGKTMLASRLADLLPEMTDEEAIETASVTSLVQNELNFQNWKQRPFRSPHHS
                     ASMVALVGGGCEN"
     gene            20979..22790
                     /locus_tag="PXB27_000585"
     CDS             20979..22790
                     /locus_tag="PXB27_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345408.1"
                     /translation="MFYLNNDNFKREPYRGYCPSHLEKLFTKPHLDIRGNGYYYPDNE
                     YVVAWYESDFIFKSRKAQAHAKVKLVNLTCPKYTGKNSAEYLSVLYISVPLNYLHYFT
                     IGSIWKGGVAKEQFALEEFYITVEAENQDGKRENLSIVSFGESEENGLEKPFDYTIYT
                     IPTEYHNYGDDLNRLLSISYQNQKFIIHPLHIFMMHYGYSTDIKRILATYPLDEVRER
                     LFIDKVVENFDVERYVVLPKYFVKKDAIFLYHFKYDEETTGTRVKKLVSQFRLNIHNQ
                     THPIEIDFWHTQKVELKIRGVRLGNAVLCAEIIGLNQPEGEDITLVLSQSKREKSGNK
                     TEEQNNEVNNVPITRVYTREPELDELPLTDNSPDNRTVEYNKRQFELLGRQRQIRALK
                     KAREESNQKMKALTPDEIDSLGVGESDGRNGKVGLAFCFLDDTPVGKSSKLYKLWQHA
                     KAVTEWNHGEAHWYTPNLGFRNDDELLPVSLETNKCFDYPEIAIIIRLQIYGETFFLI
                     DFSMKQRDISIRGLGYKPDPNEDFLYESDMSKSELSELLSAVHHHEHLPKEYIEKKNK
                     EKTKLTTFNHSEAESSNWAYNAVQKLTSRAITKPTYF"
     gene            22799..25579
                     /locus_tag="PXB27_000586"
     CDS             22799..25579
                     /locus_tag="PXB27_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HDL5345409.1"
                     /translation="MNAEQRVIVTPTFWGKFFGKIKSVELQQNKVIVTDKKNNITEHD
                     LGKTFDFPAIQKSFFGTKLFFKDDSTEVVLSKLAKKQTDSLLLEIEKVVASNIKVKVK
                     EGFQHFANLAENQYLRDSDIPTLNDRVRLSVLSYGDNKEHFQKYFDESLVKKIQYISS
                     LFPVNAINAQTIRREYEQHVLNHRKHFFDVIESNPLTEQQRLSVIRNNDINMVLAAAG
                     TGKTSVMVAKALDLIDSGACKSEDILILAYNRDAATELSERLQECAREANLDLENSPS
                     ISTFHALGRRIIKECGKSVHLSKFAEDPMKLDRWFNKWLEDYIKEDPDNLAKFIQLAY
                     QPIDPFQFKTKDEYDRYVNDNDYRTLQGERVRGYQELLIANWLFSHSVEYEYEPRYVS
                     KRRIEIGFDYKPDFSLGDGVYLEHFGIDRQGRTRADINAQEYNANIQRKRELHAEHNT
                     TLLETYHYNWVENTLYKRLEQLMNEQFIPLKPKSQQEILDALNESGIFKENKNRYLKC
                     LQAIRTERLDYQQILKRLTDAKIVYAKEYATLLMRIHDAYVKELRSANEIDFDDMILL
                     ATQLVKTGEFKPKWKHILVDEFQDISMARLELLKEIYTKGPRPIWTVVGDDWQSIYRF
                     SGGKLEVTTRFNDMVGSHTLSKLEKTYRYNNSIADTAGQFIMQNPEQYQKNVVTHTKV
                     ADSCVHLYDSHVVKDEKSEANISLKASAILKLIRQKAPEATVAILARYRYLLEDAKSV
                     IKDKNVKFWTFHGSKGLEADYCILLGFFQGKVGFPNQNKEEAVVEALLPMLDSFPHSE
                     ERRLFYVALTRAKKESYLIADANAPSEFINELLSPKYKIDIVSKRFQERYRTIFKCNE
                     CSTGYFVMKQGKFGQFYSCTSGEVCRSSPRICEKCGRPMKLREGKFGQFWGCSGYGIK
                     GDQCTNTRKYFM"
BASE COUNT         7598 a         5247 c         5520 g         7265 t
ORIGIN      
        1 aatttacttg ttgtaaggag cggttatggc aacagttaat catcaagatc gtccattgga
       61 tcttatctgt ttagggcgtg tggcggtgga cttttatggg caacaaatcg gttcccgttt
      121 ggaagagatg accacatttt caaaatacct cggcggctcg tcaggcaatg tggcttacgg
      181 tacggcggtg caaggtttac gttcttcaat gctggcaagg gtcggtgacg aacatatggg
      241 gcgttttcta cgtgaagagc tacaaagcgt aggcgtggac accagccatt taattaccga
      301 taaagaacgc ttaactgcac tggttattct cggcattaaa gataaagaga cttttccgct
      361 gattttctat cgtgataact gtgcggatat ggcaatcacc aaagacgatt ttagcgaagc
      421 ctatattgct tccagccgtt gcttagcaat taccggcacg cacctgtcaa accctaaaac
      481 ccgtgaagct gtgctgaccg cactcgaata tgcgggtaga aatgaggtaa aacgagcgtt
      541 agatattgat tatcgccctg tgttatgggg attaacctca ctcggtgatg gcgaaacccg
      601 ttatatagac agtgccgaag tcaccaaatc gctacaagaa gtattgcacc atttcgatct
      661 gattgtcggc acagaggaag aatttcatat cgcaggcggt tcgaccgata ctctcactgc
      721 actcaaaaat gtgcgtaaag tctctaatgc cgtattagtg tgcaaacgtg gggcattagg
      781 gtgttcagta tttgaaggcg agctgccgaa tgatttagac ggtggcttga atgtgtacgg
      841 cgtgcgtgta gaagtgctga atgtgctggg tgcaggcgat gcttttatgt cgggcttact
      901 gcgtggctac ttaaataacg aaggttggga acaagcgtgc cactatgcca atgcgtgcgg
      961 agcgttagtg gtatctcgtc acggctgttc accggcaatg ccgaccaaac gtgaacttga
     1021 cgattattta acccgtgcag agagtgtacc acgtccggat ttagatgagc gtttaaacca
     1081 cttacaccgt gtcaccaccc gcaaaaaaca gtgggaaaat ctctgtattt ttgcttttga
     1141 tcaccgcaaa cagttggtgg atatggcaaa taaagtcggt gcagatcttg ctcgcatacc
     1201 gaaactgaaa cagctgctat taaaagcgat ggaacaaacc gcaactcaat ccggcttacc
     1261 acaaaatcaa gcagggatcc tagccgatac cactttcggg caagatgtac ttaatgagat
     1321 taccggcaaa gattactgga ttgcccgccc gattgaagag ccgtcttccc gcccgctgga
     1381 attagagtat ggagatttag gctctcagct agcatcattc ccacgcgatc acgtcatcaa
     1441 atgcttggca ttttaccacc cgaatgatga aagcggttta aaagcccgcc aagatcgcaa
     1501 gttaaaagag gtgtatcaag cctgttgccg taccggtcac gagctattgt tggaaattat
     1561 tctgcctgct gatatgccac agagtgaggc tttctataac caagcgatag cacatttcta
     1621 ccaacttggc attaagccgg aatggtggaa attgccggat gtttctcgcg cacaatggca
     1681 agcgatttcc gccaccattg aggcaaacga tccggactgt cgtggtattt taattttagg
     1741 tttagatgcc ccgttagagg tgtttaaaga cacctttgaa caggcaaaag gctgtgagaa
     1801 agtgaaaggc tttgcagtcg gccgcaccat tttcggtgaa ccgtccgaaa aatggcttgc
     1861 cggtgaattg gacgacaata ccttgatttc tgcggtatcg gagaaatatc gcactttaat
     1921 cgatctctgg caacaacgtt aatcatattg ctttaagtga aaacccctta gcttatgata
     1981 ttaagcaagg ggttttgttt tgcaaaaaat ccggctaaac agaccgcttg ctagccgtaa
     2041 aaatgcccga attttcctaa aaaagcgaat atcgggcatt cctcaatctg cttatcacaa
     2101 aataagtgtg atgaagttca catttcatac ataaaccccc ccccttttta tcgaaacaaa
     2161 ttgttttatt tttatcaaaa tggaaaatgt atttcagttt agttctattt agattcaata
     2221 ggagtctgtg tatgagttgc aacacttcac caaaacgttt ccacgccagt tgggagtcga
     2281 tcaaacagca ccaagtacca gagtggtatg aagatgctaa gtttgggatt tttattcact
     2341 gggggattta ctccgtaccc gcctttgccc caccgacctg tcagttaggt gaaattgata
     2401 ttgatgaaca gtggttctgt aataaccctt atgcagaatg gtattttaat tccatcaatg
     2461 tcaaaaaagg cccgacctac gaacatcata tcaaaactta tggcgaatcc tttgcctata
     2521 acgacttcct gccactatgg aaagcggaaa aatggcaacc agcagaatgg gcgaaactgt
     2581 ttgccaaagc tggcgccaaa tatgtggtat taaccaccaa acatcacgat ggtttctgtc
     2641 tctatccaag taaatatacc gatttcaact gtatgcaagc cggaccgaag cgggatttaa
     2701 tgggcgagct gactgaaaat gtgcgggagc aaggcttaaa aatgggcgct tactactcgg
     2761 gcattatcga ttggacttat tcctctgccc cgattttcac cgaaagtcaa aacttctcaa
     2821 acgcttgccc gacttacgaa tacgctgact acgcctataa acaagtggta gagctgatcg
     2881 accgctataa accagatgta ttgtggaatg atattggctg gccaaaagtg ggcgagcata
     2941 tgttaccaca cctatttgct cattattaca acagcgttca agagggcgta gtggacgacc
     3001 gctggaacaa actatggtgc gatttcacct ccaaagaata caaacacggc gtggcttccc
     3061 gtgataagaa atgggaaatg tgccgtggta tgggcttatc gttcggctat aaccaggtag
     3121 aagacgaaag ccatttgatt tcggtgaaag acttgatttc cctattggta gaaaccgtag
     3181 ccgacaacgg caatttattg ctgaatatcg gcccgaaagc agatggcact attccgcaag
     3241 agcaagttga acgcttatta gcactgggga gctggctgga aataaatgga gagggaatct
     3301 ataaatcccg ttgtagccaa caccaaaccg agcaaatcgg tgatgtaacc tatcgttata
     3361 ccaaagttgg ggcggatctc tatctttaca ttgatggctt aaccgaagcc caaatcagct
     3421 tgccactgtc agtttatggt gagctacaag cactcaatac cgagctgaaa tttaccattg
     3481 aaaacaccgc acaaggcaga gtgttacata ttcaaaacta ccagccggat tggtatgtat
     3541 taggctttaa aattgcagat ggggagaaat aggtatgtcc gtttcacaac gttttattga
     3601 ctttatgaat gcccacgttg ctccagtagc acgtcgaatg gaaaatcaac cgcatatttc
     3661 cgctattcga gacggtttta ttgtagtgtt accattctta attgtcggca gctttattat
     3721 gattttgcta attccgccat ttgatgaaaa tacccaaaat gctttcgggc aagcttggtg
     3781 gcgttttgcg aattgggcaa gcccttacgg ttggaatttc ttccaaatgt cgtttaatgc
     3841 gatttcatta tttacctctg ccagtatcgc ctacaacctt gccaaagcct ataaaagaga
     3901 gcccctgcca gcggcatttc tctcggtaat ggcatttttg ttagtggctg caccggtcaa
     3961 agacggaatg atggatatta aattctttgg cggtattggg ctattctctg cgatttttat
     4021 tgccatttat tcggtagaaa tgacccgctt gttggagttc ttaaaaatta aaatccgctt
     4081 accgaaagaa gtgccgcacg ctgtagcaga atctctgaat atcgtgattc caattttagc
     4141 gattttactg actctctatc cgtttgccat ttgggtggaa agtgccacag gtcgtaatat
     4201 tccacaacta attatggact ttatggcgcc attgattgct gtgagtgact cgctaggggc
     4261 aatttgcttg ttcgttattg ctacccattt gctctggttc ttaggcatta acggctcatt
     4321 ggtactaatg caactttgga caccattcct actgcaaaat atggcggcaa acttagcagc
     4381 attccaagct ggtgaaccgc tcccctttat catcaccaat tccttctggg atttctatat
     4441 cgtacacggg gcaagtggtg gtgtaatagc cttggcattc ctgctggtac gcagtaaatc
     4501 ggcacactta cgctctatcg gtaaaatcgg cttggttccg tccttcttct ctatcggtga
     4561 accaattgtg tatggtgtgc caatggtggt taatccgctg ttctttatcc cgctgatttt
     4621 cgcaccacta gcgaatgcgg ttattgccta cctgatttta gattttaacc ttattcaccg
     4681 tgtttacttg atggcaccgt ggacaacacc ggcaccgatt ggggcatatc tggtttctgc
     4741 cggtgatatt tgggcaccaa ttttaagtat tgcactgatt attttggata ttgtgatcta
     4801 ctatccattc ttcaaaatgt atgaaaaagt ctgcgtcgag aaagaaaaag cacaaaatct
     4861 agatgaaaaa gcacaacagg agttgctaga aaaagcacga atggcagcac aataacactt
     4921 atttctagcg taattgagag gggctaatcg aaagattagc ccttattttt gcaaaaaatt
     4981 gacaaaattt atccgcttgt aaacaagaaa agcgtataat gaaataaaaa attcaagcag
     5041 agataatttg aaatgaatgg acctactcaa ttagcacatt ttcaagaaca aatccgaaaa
     5101 gagtatgacg gactaagtaa acgtttaaaa caagtggcac agtatgtgct ggataatggc
     5161 aacagcgtgg tgtttgatac ggtttctacc attgctgaac gagctgatgt tcccccttca
     5221 accttaatcc gttttgcgaa tgcgtttgga tttagcggtt ttaatgaaat taaacagctt
     5281 tatcgccaag atatgatgga aggcacatca cgttacaccg aacgtgtgca actttttcgt
     5341 caaattgaac cgcttgatga gcaaaacagt gagctcaatc atattttaga tgtgttcgta
     5401 cagggtaata cgcaggcact gcaacagtta tcgacacaag tcagcgaaga gcagttagca
     5461 caaacggtag atattttaca caatgccaaa cgcattttta ttgtgggttt aaaacgctca
     5521 ttcagcattg cgaactacct aaactatgcg ttgcaccatt tggactatga tgtctttatg
     5581 atcgatggtt cgggcggtat gtttgacgag caattaagcc gaatccgtga aggtgatgcg
     5641 gtgattgccg tgagcttttc cccgtatgcg aatgaaacac tcaacgtggt gacagcaact
     5701 gccagaccgg gggtaaaaca tattgcgatc actgatagcc aacttagccc actcctttca
     5761 tttagcgatg tgtcgtttat tattaaagaa gcacaggtga gtggttttcg atcccagtgt
     5821 gccacaatga cgctcgcaca aacgattgcc gtttcactgg cgttgaaaaa agcctagatt
     5881 aagaaagtga aagacctgcg taaatacagg tctttgtcta ttaaccgatt aaagcgttaa
     5941 tttcatcacg atactgcccg gcttttgcac caaaaataat ttgttgcgta tcaccgactt
     6001 taatgacatc aaccgcccca agctttttca gtgccgactt atttaataaa ccgttgttat
     6061 gtaacaccac acgtaaacgg gttaaacaag cttcaacttg cttgatattc tctttgtttc
     6121 ctaaagcatt aataatttct agaatgatag atttatcaga catatttcct ccatacctat
     6181 ttatatttaa aactcttatt atgatatttt aaacattttc tgatggttag aacaattttt
     6241 attagtaatt tgtataaaaa aacgctaaat gtatctttct ttaaaagagt catatcaatg
     6301 aatctccctt gtctttccgc aaaaaatctg tataatttga ccgcttgttt tctcttttga
     6361 aacaagttcg ttaccctcac ctcgaacgag ggtttatttt ttactatcaa catttagagg
     6421 aaaggttatg gtaaccattc gtttaactcg tggcggagct aaaaaacgcc cattttacca
     6481 aatcgtggta gtggacagcc gttcacctcg tgatggtcgc tttatcgagc gtatcggttt
     6541 cttcaaccca ttagcagcag gtcaagctga gcgtttacgc ttagatgtgg ctaaagtaga
     6601 tgcttgggtt gctaaaggtg ctgatttatc agaccgtgtt gcttctttag taaaagaagc
     6661 tcgcaaagca gcttaatttt taagccaaaa ttaaataagt aggtgagtat gagcgaacaa
     6721 aagatcgaag ttgtcggaaa attaggttca acctatggta tccgtggttg gttgcgtctc
     6781 tattcatcga cagaacaaac tgagagcatt ttcgactacc agccgtggtt tttaaaaatt
     6841 aaaggtcagt ggcagcaggt cgaattagaa agctggcgtt atcatagcaa tgatttgatt
     6901 gttaaattga aaggcactga agaccgtgaa gccgcacagt tattaaccaa cgctgaaatt
     6961 ggcgtggatt tgtctgtgtt tcctgaattg gaagagggag attactattg gcacgatttg
     7021 atcggctgtt cggtagtaaa cttagagggc tacacaatgg gaacagttgc cgaaatgatg
     7081 gaaacaggtt caaatgatgt gcttgtggtg cgtaataacg gcaaagatgc tttcggtaaa
     7141 caagaacgat taattccgtt tttgtatgaa caagtagtta aaagagtaga tctcgccacc
     7201 aaaaccatta cggtggagtg ggacgctggt ttctaacctc aggtatgcgg tgagctttgg
     7261 aaaacattta aggcgttaaa atgtggattg gtattatttc actgtttcct gaaatgttta
     7321 aagcaattac cgatttcggg gtgacaggac gtgcagttaa acaaaatcta ctgcaagttg
     7381 aatgttggaa tcctcgtgat tttacattcg ataaacataa aactgtagat gatcgccctt
     7441 atggcggtgg tccgggaatg ttgatgatgg tgcaaccttt gcgtgatgcg attcacgcag
     7501 cgaaaaaggc ggcattagca gtagaaggcg gtgaagctaa agtgatttat ctttcgccgc
     7561 aaggacgtaa actcgatcaa aacggtgtgc aaacactggc ttcaaatcag aaactgattt
     7621 taatttgtgg acgatatgaa ggcgtggacg agcgattgat tcaaacagaa gttgatgaag
     7681 aatggtcaat cggtgattac gttctcacag ggggggaatt acctgcaatg acattaattg
     7741 atgcagttgc aaggtttgtt cccggggtat taggcaaaca ggcttccgcg ttagaggatt
     7801 ctttcgcaga gggattatta gattgcccac attatacccg ccctgaagta ttagacggta
     7861 tgcctgttcc ggaagtgctg atgtctggac accatgaaca aattcgtaaa tggcggttgg
     7921 aacaatcgct ggaacgaacg tggttaagac gccctgagct attggatagc ctagctctga
     7981 ctgacgaaca gcgtgtgttg ttagctaaaa ttaaaaaaca gcacaaaatc agttaattct
     8041 aggattatag aaggttttta aaatgagtaa catcatcaaa caacttgaac aagaacagtt
     8101 aaaacaagac ttacctagct tccgtccggg tgacacatta gaagttaagg tatgggtagt
     8161 agaaggtagt aaaaaacgtt tgcaagcgtt cgaaggcgtg gttattgcaa ttcgtaaccg
     8221 tggcttgcac tctgcattca ctctacgtaa agtttcaaat ggcgtaggcg ttgagcgtgt
     8281 attccaaact cactcacctg taattgacag catttctgtt aaacgtaaag gtgcggtacg
     8341 taaagctaaa ctttactact tacgtgagcg ttctggtaaa tctgcacgta tcaaagagcg
     8401 tcttggcgac taattgctac aaaacagtga aatgcacaaa agccttgagg aaactcaagg
     8461 cttttgcttt ttgtacttac aagcagttat ttttcctcaa tgatttacaa atcttgcttt
     8521 cttttttgtt ataatgttaa caagataaca ttaatgatag gtgtcgatat gaaacaatta
     8581 agtattagat tgccagacga tgaatttact tttattaaaa catttgcttc tgctcataaa
     8641 ctgactttac aaaattttgt gatgtccgca atccaagcta aagttgcaga aatgtctgct
     8701 aaaaataaaa tatctgaaga aagtaacatt catcaacctc tttctaaaga tgaaaaaata
     8761 atgctaaatg aggcaaaagt attttattct gctcgttcgc atttaaaggc agaaaatgtg
     8821 attcttccga gtgattttat ggagcaaatt aaatcaggct gtggagcaga aaaaatggca
     8881 gaaatgatgg gatataccaa agaagattgg gagtcaatga agtaatatgg ggcagattac
     8941 atttagtaat attttcacaa aagtgatttt tgagaatgtg ctttctgctt caactaaaga
     9001 gaaaataaaa caaatgcttt ttcagtctgt ggttgcatta cctcctctcc attcagaaag
     9061 aaaaatgatt ttacagttga aaaagcaaaa aattaccatt ttttatcaag ttattgagga
     9121 acaatctgtg caaatcctcg cttatcaatt tggtggaact gataaattga caggtgtgtc
     9181 taaagctcac tttaaaacac tagatgccat ttttcaaatg acggatgaag agaaagaagg
     9241 aaaattctaa tgtaagcggt tatttttcca tatttttttg caaataacgt ttctcaaatt
     9301 ttgcttgatg gcgttcaagc acttccgtca tcgaaatgcc atatttatcg gctaatacaa
     9361 aaagattatc catcacatcg cctaactctt cgattaagtt atctcgtttt tgctgttgtg
     9421 aagcttcggt ttcgtccggt cgttcacgcc cgatttcaat cgctcttact gcacaagcca
     9481 cttcgccgat ttcttctgct agataagtca cccgcataaa agggcttcgt tcataccaac
     9541 cttgttgttg gtaaaactgg cgaacccatt gttgatattg ttcaatttgc attttatttc
     9601 tgattcaaaa agtagggacg aaaaattttt cgccctttta taagaaaatg tatcagggca
     9661 aatgatgatt tgcccctagg agtgttactc tgcctcagag agaataatcc agccactatc
     9721 cagccagtcg caaatggtgt ctagcattag ctccagctcc gcttggtctt gcactttgga
     9781 ggcaagtttg ttccacgaaa tcgaatctcc attggcgata cgaatcagta atgccatttc
     9841 tgcttcgttt aactcatcaa tccactcgcc atttacataa acccgttgag ggttctcggt
     9901 gtagaggatt tttacatttg catcttgctg taaccagccg ccggcttcga gaatttcttg
     9961 cacttcgtca gggtaatatt cattgtcagt ttgcaataat tcataacggc gggagcttgc
    10021 cgcagtggca acactgtgag tgagtaactc atcgaactgt tcggaatttt ggagcaagtc
    10081 gattaactga tgtttaaaca ttttcaccat tttcggatca agcaacgcat tcggctgttc
    10141 attcggcgtt aagcggaacg gaagatgaaa ttccgatgaa ttaatgttgt cggcttgttg
    10201 ttcgatggtt ttgcagagat tttccagtaa atcggtcgca tttggataac gtaaaccaaa
    10261 agagaaagtt aaaccttgct cgctttcgga aacaccatag tgagccattc gagacggcac
    10321 ataaagcaca tcacccgggt tcattacctc gtccattacc agttcgccca tatcatcaaa
    10381 aatgcggatc ggttggtttg gtttgaattc ggtgcttgga tcgcaccatt tgcctaattg
    10441 ccaacgacgt tgcccatagc cttgcaccaa gaacacatcg tactcatcat aatgtttgcc
    10501 gacagtgccg ttgagcggtg agcaagaaac cataatgtca tcacgttgcc attgcgggat
    10561 aaaaccaaaa gcctgccaaa gctcgcctag ctcaggagac cattgctcga ggttttgcac
    10621 cattacagac cattgtacag gtaactcttc caaatcttcc tcaacaagcg ggctggattt
    10681 aacagaccat ttctcgtttt cgcattgaat caggcgtgca gtgacttctt cctctaatgc
    10741 cagttctaaa atatcttccg gctcaaacaa tccgacaatt tggggtaagc cgttacgaat
    10801 taatagcggt cgtttttgcc aatattctcg caaaaaggtt tcagggctga tattttcagg
    10861 taagcagaat ggaatgttca taatggctcc ttgctacgtg cttctttgag attactgata
    10921 ttctacccga gaaatcagga aaaacgggaa attttttatg atttagcgac atcattcaca
    10981 ttttgaagag gaaataatga aaaaggcgaa cattgttcgc ctttgaaatt tattttaaac
    11041 cgagtttttt ctcgagatag tggatattgg tgccaccttt gcggaattct ttatccgcaa
    11101 gaatttgagt atgtaaagga atattggttt taatgccttc cacaatggtt tcggaaaggg
    11161 cattttccat ccggcgaatc gcaatatcac gattttcagc aaaggtaatc aatttaccaa
    11221 tcattgaatc gtaatgtggc ggcacgctat agcctgagta aatatgtgaa tcccaacgca
    11281 cacctaaacc acccggaacg tgtaaacggg taattttacc cggcgatggc aagaaggtgt
    11341 ttggatcttc tgcgttgata cggcactcaa tggcgtgacc tttcacttta atatcatctt
    11401 gtgtgatcga aagtggtaag ccggctgcca cacgcagttg ctcttttacc aagtccacac
    11461 cggtaatcat ttcggttaca gggtgttcta cctgtaagcg ggtgttcatt tcaatgaagt
    11521 aaaactcgcc gttttcatat aagaactcaa aagtacctgc accacggtag ccaatttcac
    11581 ggcaggcatt ggcacaacgc tccccaataa attgacgcaa ttcaggtgta ataccctgag
    11641 caggtgcttc ttccaccact ttttggtggc gacgttgcat tgaacagtca cgctctgcta
    11701 aataaaccgc attaccgtga gtatcggcaa gtacttggat ttcaatatgg cgtggatttt
    11761 ctaaatattt ctccatatac accatatcgt tattaaatgc cgctttcgct tcggctttcg
    11821 tcattgcgat agattcttcc aaatctttct cgtgatgaac cacacgcata ccacgaccgc
    11881 caccaccacc tgatgctttg atgatcaccg ggtagccgat acgtttagcg atttctttat
    11941 ttttcttcgg atcgttacca accggaccgt cagaacccgg tacgcaaggc acgccggctt
    12001 ttttcattgc attgatagca gagactttat cgcccattaa gcgaatcaca tcagccgttg
    12061 gaccaataaa gataaagcct gatttctcaa cttgttcggc aaagtttgcg ttttcagata
    12121 agaaaccgta gcccggatga atagcatcgg cattcgttac ttcggcagcg gcaataatca
    12181 tcgggatatt taaatagctt tttaccgatg gagcaggacc gatacaaatg gtttcatcgg
    12241 ctaatttaac gtgttttaat tcacggtcgg cagttgaatg cactgcaacc gttttaatgc
    12301 ccaattcttt acaagcacgc aaaatacgca gggcaatttc accacggttt gcgataacaa
    12361 ctttttctaa catagggaaa ttccttgtgt tctagaaaat gtagggcgtg ttgcacacgc
    12421 ccgttggata atttaaggaa gagcttattc aatgatgaat aatttttggt cgaattctac
    12481 cggttcgcca tcattaacta agatttcttt taccacaccg gctttgtctg actcgatacg
    12541 gttcatcatt ttcattgctt ctacaataca taacgtatca ccgattttca cggtttgacc
    12601 cacttcaacg aacggttttg catctgggct tgggctacgg tagaaagtcc ccaccattgg
    12661 tgaaagcact gcgtggccac tcgtttctgc ggcttttggt gcttcagccg gagctgcaac
    12721 aggtactgtc gaagctactt gaggtgccgc tggtgcaaca acaggagctg ctgcatattg
    12781 aactgcagct ggtgcaacag ctggagcagc acggctaata cgcaccgtac cttcttcttc
    12841 agacacttct aactcggtaa tgccagattc ttcaactaat tcaataagtt ttttgatttt
    12901 gcgaatatcc ataataagat tccgtatgtg attaaataaa ataaaaaaga gaactccaca
    12961 tttggggtat aaattcaaat gtagagttaa aaaatgctgg gcgtagaata cctgaatttc
    13021 gctaattttg cgaataaaat ctgataattt tggcgaaaat ggacgatttt agcattcatc
    13081 gtaccagtta ggatttttgg aaaatggtga ctcacaagcg attgaaaatt gccaattttt
    13141 tgtaaaaaac aaccgcttgt cagattattt caataaatct tcatttttct tgtaattcca
    13201 cttgctaccg ggcttgtcga tatactgaaa atattcgttg gtaaaagtac cttgagcaca
    13261 agttactttg tctcggcgga aatttacttc tttatcacca atggcattat ttagggtgtc
    13321 aggaccggtc ataacaaaca cgccaccttc aatccgacga ttctcaatat tgctgataat
    13381 cagctcaagg gtgtctttta gaatccaatt tccagcttcc gaagccaaga aaaagttggt
    13441 gtatttatca cggcgtttaa ttaacacttc gctatcattt tcgccaataa ttttagagag
    13501 tgggtaaacc aaatgcccgt caatatcaat ataaatgcca ccttctcgga gcaaagtaaa
    13561 aattcgccaa aaatcagctt gtgccgcacc atcggttaat ttgctatagg cgttaaaggt
    13621 gcgttcatcg gcgttggcct tgatgtatgc ctctcgttct tctgtgctaa cgtagcgata
    13681 atcgtagctc aatgacatta ggcggttaaa cagataattc gcatacattg gcaaggtgac
    13741 tttgttagaa taattcgttt gccaaatggt tttggtaatt ttgctcggaa tgcttgattg
    13801 gatttttgcc gggctatatt ccggaatggt gaaacgtttt ttcggaaaaa gtgcatggaa
    13861 cggataactc aatattttga cgatattacc gaataaacgg aagactccgt tacagatcac
    13921 aataaattgt tcatttttaa cagccatttt attaccttat gcttttaagt gtgaaattgc
    13981 aaaatccaac gcatattcat agccttttgc ccctaaaccg caaataacgc cttttgcaat
    14041 atcacttaga taggagtgat ggcgaaaagg ttcacggcta tgaacgttag acaaatgcac
    14101 ctcaacaaac ggaatcgaca cactcaataa cgcatcacga attgccacgc tggtgtgagt
    14161 aaatgccccg gggttaataa taataaaatc ggtatgctga aaggcttggt gaatacggtt
    14221 gataatcggc tcttcaccgt tggcttgaaa gctctccagc tcaacatctt gggccgcagc
    14281 caattttttt aagtggtttt caatatccgc caaagtttgc gaaccgtaaa tttgtggctc
    14341 acgtttgccc aacatattta aattcgggcc gttaaggaga aggatttttt tcatatttac
    14401 ttattcaact tgtaattcat aattaagcca ttcacggttt tctttcgccc ccaagcgttg
    14461 ataaaaacga ataccaccgt cattccaagg attttattgt cttacaatga tgtgccttcg
    14521 cctcattttt caaggcttgc attaacgcct ctcccacttt ctgattccga gcagtttcat
    14581 caacataaag ctctttcata taaatagcgg ggcggttttg ggcagtgtaa ggaagaaaat
    14641 aataaaccaa tatgccgata atgttagacg cattttcagc cactaaacag taaaaatcgg
    14701 gtgggctttt gcgaaagccg gattccatta caacttcagg agtaatcgca aaagaatcga
    14761 tatagtgctc aaacacagcc agtttttgca tcagagggaa cacttggtgg gtatcttgtt
    14821 cgaccatttt gcggattgta aatgccataa aattccttac aagcggtaaa aaaatccctc
    14881 acttttgcaa agcgagggag gttttaatta ttcgtcaata gaacgtaata agtcgtttaa
    14941 gcccactttg cttaaggttt tctcatccac ttttttcaca atcaccgcac agtagaggtt
    15001 gtgtgaacca tcttttgatg gaagcgagcc tgaaaccaca acagagcctg ccggcacacg
    15061 gccgtaatgt acttcgccgg tttcacggtc gtaaattttg gtagattgac cgataaacac
    15121 gcccattgag atcacgcagt tatcttcgac aatcacgcct tctacgattt cagaacgtgc
    15181 accgataaag cagttgtcgc cgatgatggt tgggttcgct tgtaatggct ctaacacacc
    15241 accgatgccc acgccaccag aaaggtgaac atttttaccg atttgcgcac aagaacccac
    15301 tgttacccaa gtgtccacca ttgtgccttc gccaacgtat gcaccgatgt tcacgaaaga
    15361 tggcattaac accacgtttt tctcgatatg tgaaccttta cgcaccaccg cacccggcac
    15421 ggcacgaatg ccatctgctt tgaattgctc ttcggtgtag ttgccgtatt tggttggcac
    15481 tttgtcgtag aatttggttt ctgcaccgtc gatgatttca ttgtcggcaa tacggaatga
    15541 aagtaatacc gcttttttca cccattggtt tactacccac tcgccatctt ttttctcggc
    15601 aacacgcagt gaaccgttgt ctaatcctgc gatcacctct tctactgctg ctttggtttc
    15661 agcagcaacg gttttcggag taatttccgc acggcgttca aatgccgctt caataatagc
    15721 ttgtaaagac attgttgtgt tccttcaata tataaattaa aacagaaaat tcggctcact
    15781 ttttaacata gaaaggcgtt tatttccaca agaaaaaaca tatcgcagaa atttagttta
    15841 taattccaag ccacttattt gatacaagcg gtgatttttg tggaaaattt tgcaaatctg
    15901 cttaccggta aaacgcgtga gcatttagtg ccgttgccga acgctctttc ggataaacat
    15961 taccttcagc ctcaggtggt tgaggctttt ttgacgttgc agcatgctgc aaaacaagcc
    16021 ggctttaatt tacagcctgc cagtactttt cgtgattttg aacgccaaaa agggatttgg
    16081 aatgcgaagt ttaggggtga gcgtaaggtt cacgatgata acggggttgc gattgatatg
    16141 gcggcgttat cggatcttga aaagtgcaaa gcaatgttga gatggtctgc ggtgccgggg
    16201 gcaagtcggc atcattgggg aacggaaatt gatatttttg accctgattt attgccggaa
    16261 aatacgcctt tattgcttga gccgtgggaa tatttggaag gtggctattt tgccgattta
    16321 accaagtggt tacacgttaa tgcggaaaca tttggctttt atttcccgtt tgacggtaaa
    16381 cattctcgag tgggctttga gccgtggcac attagctatc gcccgatttc aaatgaatat
    16441 gagcaacgct ttaacgatga aatcttaaaa gctgcgtggc aagatgaaga agtggcgggt
    16501 aaggaatgct tacttgaaca ttttgaagaa ttactaaaat gatccaatta attaacgaaa
    16561 cccaacaaca cgacaaattc cgccaaattt gcgacaaatg gcagctggtt gaagataaat
    16621 ccagcccgtt agccttagtg ctaacggagg aacgattgga actacgcaaa ttagacgaac
    16681 ccaagctcgg ggcgattgca gtgaattttg tggacggtac gatggcacat cggcgtaaat
    16741 tcggtggtgg gcgaggtgaa gccgttgcga aagcggtcgg cattaaaggg gattatttgc
    16801 caactgtgat tgatgcgaca gccggtttag ggcgagatgc ttttgtatta gcagcggttg
    16861 gttgtaaagt gctgttagtg gaacgcaatc caattgtcgc tgccttgttg gaagatggtt
    16921 taaaccgagc ttatcaagat gtggaaatcg gcagttttat gcaagaaaga atgatcttag
    16981 ccgatgttcg agacatttct cagctagatc cacaatccca aatggctgag gtagtgtatt
    17041 tagatccgat gtatccgcac aaacaaaaaa gtgcgttggt gaaaaaagaa atgcgggtgt
    17101 ttcaacattt agttggggca gatttagatt cggacagctt tttcttaccg gcaaaaggat
    17161 tggcaaaaaa acgtgtggta gtgaagcgtc cggactatgc cccgttttta gcggagcaaa
    17221 agcctgattt ttcacacacc accaaaaatc accgttttga tgtttatctt tctcacctga
    17281 aaaatgatta aaggtgggtc atgcgatagg caagcggtta tttttacagt aaaatttaca
    17341 aattatgtta tcgtttattt atatttaaaa aggcggttat cgtgccacaa cgtgattatg
    17401 tcgctcgttc caacgagaaa aaaagtaaaa aaaatcaaaa agaaagtaat gttaaaaaga
    17461 ttttaatgcg gattatcggg gtaattttat tcttcggtat tggtttatgg tttttgaaat
    17521 ccagtgcacc tgtaccaaca gttccgtcag caccgcagcc tgctaattca cagactaaaa
    17581 gcgaattacc aagtcgaccg gaagagacgt ggagctatat tcgtgattta gaaacccgta
    17641 cggtaattac cgataatagc caaacttcac aagaacgttt ggcacaatta acggcggaac
    17701 aaaagcagca aattgaagaa agacgcaaac aagaagaagc acgcttagcc gctgagcaag
    17761 ctcaacaaaa gcctgcgacc gatgtatctg ctacagaatt ggctccgaaa acagaggaag
    17821 ttaagtcgtt aacggaagaa gaattagcag caaaaaaagc acaggagcaa gctttagcac
    17881 aaaagcaagc ggaagagaaa cgcagacaag cagagcttaa aaaacagcag gaagcgaaaa
    17941 aagagcagcc ggctaaaatt gtagcaagtg ctgcaacact ggcagataaa gctgcaaatc
    18001 aggcgggtaa atttgggtta cagtgcggtg catttaaaaa taaagcccaa gccgaaaata
    18061 tgcaagcaag attggcaatg gcaggtttta acgcccgtat cgcaagcagt gccgattgga
    18121 accgtgtttt tgtcggcccg ataggtgaca gagcggcaac ttccagagct caatccaatg
    18181 cacgcagtgt agcggaatgt gtgattgtgg cgatgtaaaa ttgattgaaa tattgaagag
    18241 agctataggc tctctttttt tatatcccaa tcgcatattt caaggcttgc tttttgatgg
    18301 gtgacacttt atcagccaat actaaaccga cattgcgggc aattttgagc ggtaataaat
    18361 cggtcttaaa cgctttataa aacacgtcca tgcctgtttg catcagtaaa ttatccggtt
    18421 tacgtttttg ctcatagcgt tgaagcacct gctcacttgc aaaatttttg ccttttttga
    18481 ccgcttgttc gatcacctca agcaaggttt taacatcttt aaagcctaaa tttacacctt
    18541 gtccggcaag cggattaatg gtgtgagccg catcgccgac taataccacc ccgttttgca
    18601 catagtgctg agcgtgctgg cgggttagcg ggaaagcccc tgaagcctgc acctgaatgt
    18661 tgcctaattc tgcgttgagc cggcttggga aatgttgctc aatcgcaact cccagctttt
    18721 ctgctggaag cattttcagt tcggcaattt tttgtggtga atcgtaccaa acaacgcagc
    18781 cgtgattgcc ggaaagcggt aaaaaggctc gagggccggt tggatagaat tgttgccaag
    18841 tgccgcttgg agcaggtttt tcggtggtaa cggtggcgag taaacagtgt tggcgatatt
    18901 gccaagcggt taaaccaatg cccgcccatt ggcgaacctg tgaatttgca ccgtctgcgg
    18961 caatcagcag tggggcagca aaggtgttgc catcaaccgt cacttcccaa tactcgcccg
    19021 ctttatttgc aaaaatttgc tcaaatccga ccgcttgtag gcagtttgga tattgcttta
    19081 attgctccca taacgcaatt tgaataacgt tgttttcgac catatcgccg agcttttcca
    19141 aacccaaatc agctgccgaa aaaagagtgt tgaatccatc aatttcccag gtttcgagct
    19201 gggtataagg ggtgcttctc attgcaacaa tttgctccca cacgcccaat ttttctaaca
    19261 gttgaatcga gcccatacta atggcggaaa ttcgcaaatc gtagtctgaa tcaggattaa
    19321 aggtcggcag agggtttttc tccaacaagg ctaaacgcag cccctgtttt gctaagccta
    19381 gagctgttgc tgccccaatc ataccgccac cgattacaat catatctaat tcagttttca
    19441 ttttttcttc ctttttagtt aggcaaaata ctgctaaaac gccttgaatt gtagtgcatt
    19501 tcagattagg atcaaaccga tttatctatt tatctaagga gcgttttatg tcttgcaaaa
    19561 ctcatgcaaa ccacgagcac gaacacggcg aaggctgtgg tcatactgct attaaacata
    19621 acgaccatat cgactattta cacgatgggc atttacacca caacaaacac ggcgatcact
    19681 atgacgaaca cgttttagaa gtgaatgaaa ccaacccggc agactgtaat gccgcccatt
    19741 gctgcggcga ccacgttcat ggtgaggttt gcggacacga agctgtgccg cacggcgatc
    19801 atatcgacta catcgtgaat gggcgtttgc accaccaaca cggcgaccac tgcgatgacc
    19861 acgggccggt tgaaattgtg aaataagatt tttgatggga ggttttagcc tcccattatt
    19921 atttttgcga tctcgctcga aaaattgaac attcctgcta cccctcttcc gattttcctt
    19981 ttatgctctc ggtattgatt tctaataccg aaatgtgatg accttagcga ttgtttacag
    20041 ccgagcctcc attggcgttg aagccccgtt ggtgacgatt gaagtgcata ttagcaacgg
    20101 acaaccaaaa ttaaccattg tcggcttgcc cgaagccaca gtgaaagagg ctggtgatcg
    20161 tgtgcggagt gcgttgctga acgctaattt tatctatccg ccacaacgga tcacgattaa
    20221 tcttgctccg gcagatttac ccaaagaagg cggacgtttt gacttgccga ttgcgattgg
    20281 cattctggca gcctcagggc aaattgattc tgatttatta acgcagttcg agtttttggg
    20341 cgagttggca ctgacagggc atttgcgagg agtgcacggc acgattccgg cagtgatttc
    20401 tgccgataaa gccaaacggc agatgatctt agccaagcaa aatgcgaatg aggcatcgct
    20461 tgtttccaat gccaccacct actttgccgg ctcgttgttg gaggtggtaa atatgctcaa
    20521 caaacgggat aaactgccga tttgtcagca catttcgcaa cattctgccg aaattcgacc
    20581 gcttgtcagc cgagatctta ctgatattat cgggcaacaa cacgccaagc gagccttaat
    20641 gattgccgct gccgggcaac ataatttgct gtttctcgga ccaccgggaa cgggtaaaac
    20701 aatgttggca agccggctgg cggatttact gccggaaatg accgatgagg aggcgattga
    20761 aaccgcctcg gtgaccagcc ttgtgcaaaa tgagctgaat tttcagaact ggaaacaacg
    20821 cccgtttcgt tcgccccatc acagtgcatc aatggtggca ttagtgggag gtggctgtga
    20881 aaactagaaa taaatgtcaa aaacaagaaa taaatgtcac aaatctttct ctaatttcgc
    20941 tataattcga tcatcaaaac tttaagggga aatccgttat gttctacctc aataacgaca
    21001 atttcaaacg agaaccttat cgtggatatt gtccgagtca ccttgaaaag ctttttacta
    21061 aaccacacct tgatattaga ggtaacggtt attattaccc tgacaatgaa tatgttgtag
    21121 cttggtatga atcggacttc attttcaaat caagaaaagc tcaagcacat gcaaaagtaa
    21181 agcttgttaa tttaacttgc cccaaataca ctggcaaaaa cagtgctgaa tatttaagcg
    21241 ttttgtatat ctccgttccc cttaattatc tacattattt cactatcggc agtatctgga
    21301 aaggcggtgt cgccaaagag cagtttgctc tcgaagaatt ttacattacg gttgaagctg
    21361 aaaatcaaga tggtaaacga gaaaacctct caatcgtttc attcggtgag agtgaggaaa
    21421 acggtttaga gaaaccattt gattacacaa tctacactat ccccacagaa tatcacaact
    21481 acggtgatga cctaaaccga ctactttcca tctcttacca aaatcaaaaa ttcattatcc
    21541 atccgctaca tatttttatg atgcactacg ggtactctac ggacatcaag cgtatcttgg
    21601 caacataccc acttgacgag gtaagagaac gcctatttat agataaggtc gttgagaatt
    21661 ttgatgtaga gcgttatgtc gttttaccga aatactttgt aaaaaaagat gcgattttcc
    21721 tctatcactt caaatatgac gaagagacta cgggaacaag ggtaaaaaag cttgtaagcc
    21781 agtttaggtt aaatattcac aatcaaacac atccaataga aatagatttt tggcacacgc
    21841 aaaaggttga attgaaaatc cgtggtgttc gacttggaaa tgctgtgtta tgcgcagaaa
    21901 ttatcggtct taatcagccc gaaggcgaag atattacgct agtgctatct caatcgaaaa
    21961 gagaaaaatc gggtaataaa accgaggaac aaaataacga ggtaaataac gttcctatta
    22021 ccagagttta tacacgagaa cctgaattgg atgagttacc actcaccgat aactccccag
    22081 ataatcgaac agtcgaatac aacaagcgac aatttgaact cttgggccgt caacgccaaa
    22141 tcagagcgct caagaaagca cgcgaagaga gtaatcagaa aatgaaagcc ttgactccag
    22201 atgagataga ctcactgggt gtaggtgaat cagatggccg aaacggtaag gttggactcg
    22261 ctttctgttt ccttgatgat acacctgttg gcaaaagctc caaactatac aaattatggc
    22321 agcacgcgaa ggctgttacg gaatggaatc acggtgaggc gcactggtac acgcctaatc
    22381 tcggttttag aaatgacgat gaactccttc ccgtttcctt agaaaccaac aaatgtttcg
    22441 actaccctga aatcgcgata attatccgcc tccaaatata cggagaaaca tttttcctga
    22501 ttgatttctc aatgaagcaa agagatataa gcattcgtgg cttagggtat aaacccgatc
    22561 caaacgaaga ttttctctat gaaagtgata tgagcaaaag cgaattatcc gaattattgt
    22621 ccgcagttca tcatcatgaa catctgccaa aagagtacat agagaaaaag aacaaagaaa
    22681 aaaccaaact cactacattt aaccatagcg aagccgaaag tagtaactgg gcttataacg
    22741 cggtgcaaaa attaacttca agggcaatta ctaagccgac atatttttaa ttatttttat
    22801 gaatgcagaa caacgtgtaa ttgttactcc gacattttgg ggtaaatttt ttggaaaaat
    22861 taaaagtgtt gaacttcaac aaaacaaagt tatcgtaact gacaaaaaga ataatataac
    22921 tgaacatgat ttaggaaaaa cttttgactt tccagctatc cagaagtctt tctttggtac
    22981 taaattattt tttaaagacg atagcacaga agttgtttta tcaaaacttg cgaaaaaaca
    23041 aactgatagt ctattacttg aaatcgaaaa agtagttgca tctaatatta aagtgaaagt
    23101 aaaagagggt tttcaacact ttgcaaattt agccgaaaat caatatctca gagattccga
    23161 cattcctaca ttaaatgatc gtgtaagatt gtcagtgctt tcctatgggg ataacaaaga
    23221 acattttcaa aaatactttg atgaaagttt ggttaagaaa attcaatata tctcttcact
    23281 ttttcctgta aatgccataa atgctcaaac aattcgaaga gaatacgagc aacatgtttt
    23341 aaatcatcgc aagcatttct ttgatgtcat tgaatctaat ccgcttactg agcaacaaag
    23401 attgtcagtc attcggaaca acgacatcaa tatggttctt gctgccgcgg ggacaggaaa
    23461 aacgtctgta atggtcgcaa aagcattgga cttaattgat tctggtgctt gtaaaagcga
    23521 agatattttg attcttgcat acaaccgcga tgcagcaacc gaactatccg aacgcttaca
    23581 agaatgtgca cgagaagcca atttagatct ggaaaatagc cctagcatct cgactttcca
    23641 tgcgcttggc cgaagaatta ttaaagaatg tggaaaatca gtccacttgt caaaatttgc
    23701 cgaagatccg atgaaattag atagatggtt caacaaatgg ctagaggact acatcaaaga
    23761 agatcctgat aatcttgcaa aattcattca acttgcatac caacctatcg atccattcca
    23821 attcaaaacg aaagacgaat acgatagata tgtaaatgac aatgactatc gaaccttaca
    23881 aggcgaacgc gttcgtggtt atcaagagtt attgatcgcg aactggcttt ttagccacag
    23941 cgtagaatat gagtatgaac caaggtatgt atcaaaacgc cgtatcgaga ttggttttga
    24001 ctacaaaccc gatttctctt tgggtgatgg tgtttacctt gaacatttcg gcattgaccg
    24061 acaaggtaga actcgcgcag atattaacgc gcaggaatat aatgccaata tccaaagaaa
    24121 acgtgagtta cacgccgaac ataatacaac attactggag acataccact acaactgggt
    24181 ggaaaatacg ttatataaac gactagagca attgatgaat gaacagttca ttcctctgaa
    24241 gccaaaatca caacaagaga ttttagatgc actcaatgaa tcaggaatct tcaaagaaaa
    24301 caaaaatcgc tatttgaagt gccttcaagc tattcgtaca gagcgattag actatcaaca
    24361 aatcttaaaa cgtctaaccg atgcgaaaat tgtctatgct aaagaatatg cgacattgct
    24421 catgcgtatt catgatgcct acgttaaaga actacgtagc gcaaatgaaa tcgactttga
    24481 cgatatgatt ttgttggcta ctcaactggt gaaaacaggt gagtttaaac ctaaatggaa
    24541 acatatttta gtcgatgaat ttcaagatat ttctatggct cgattggagt tattgaaaga
    24601 aatttatact aaagggcctc gaccgatttg gactgttgtt ggagatgact ggcaatcaat
    24661 ctatcgtttc tctggcggta aactagaagt tactacgcga tttaatgata tggtcggctc
    24721 acacacgcta agtaaactag aaaaaaccta ccgctataac aatagtattg ctgacacagc
    24781 aggtcaattc attatgcaaa atcctgagca gtatcagaaa aatgttgtaa cgcatacaaa
    24841 ggtcgctgat tcttgtgttc atttgtatga ctctcatgtc gtaaaggatg aaaaatcaga
    24901 agccaatatc agtttaaaag cctcagcaat attaaaactg atacgtcaaa aagcacctga
    24961 agccactgtc gcaattttgg ctagataccg ttacctttta gaagatgcaa aaagtgtaat
    25021 caaagataag aatgttaaat tttggacgtt ccacggctca aaagggttgg aagcagatta
    25081 ctgcatttta cttggtttct tccaaggaaa agtcggattt cccaaccaaa ataaagaaga
    25141 agccgttgta gaagcattgc ttcctatgct agacagtttt ccacactcag aagaacgtag
    25201 gttattttat gtggcattaa cgcgtgcgaa aaaagaaagt tatttgatcg cagatgcgaa
    25261 cgcgccatca gaatttatca atgaattatt atcaccgaaa tacaagatag atattgtgtc
    25321 aaaacgattc caagaacgtt atcggacaat ctttaaatgt aatgaatgta gcactggcta
    25381 tttcgtgatg aaacaaggta aatttggtca gttctattct tgtacatcag gggaagtatg
    25441 ccgctcttcg ccacggattt gtgaaaaatg tggccgtcct atgaagctgc gagaaggtaa
    25501 atttggtcag ttttggggat gctcaggata tggaatcaaa ggcgatcaat gtacgaatac
    25561 tcgtaagtat tttatgtaat atattatttt ggaggatttt gtggatttat ttgaatcaaa
    25621 aaaacaatta
//
LOCUS       DANLUQ010000017        24271 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.017, whole genome shotgun sequence.
ACCESSION   DANLUQ010000017 DANLUQ010000000
VERSION     DANLUQ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 24271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24271
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.017"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(108..1220)
                     /locus_tag="PXB27_000587"
     CDS             complement(108..1220)
                     /locus_tag="PXB27_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345410.1"
                     /translation="MKNNKTDTLLFLEQFARYLTAKSWQIAFELEYAVIWQKDLGHRI
                     LEVTLPKPIAEDREEVLQKALEKLAKIEEKNVETLKLNISNQYTDKLSVRVIGESVKD
                     GTIPLNEGVKLFEKTKHLINALALSAKKKKAAFGHNSGGKDVAEFMSQVRLGQTQIGS
                     YIVNLSYPVENIEIKEQNEIIQAISFSRSVSHNLVNSLNKLKEKITHYDHNPTIFAEL
                     IPEGVSHNLCEAIIGLSGSDQQRKIEIKLQVGEISDESMPSETFNIKFSQKEIKVIQV
                     ASRYYQGEYTLPHYEIIGKVVGLHSSNLADGGYIQVPCKIEDKTVDIRVDLPPEQYKL
                     AAEAHKTEQQIRCKGENLYINKKKGRLQKLTSINIL"
     gene            complement(1217..1720)
                     /locus_tag="PXB27_000588"
     CDS             complement(1217..1720)
                     /locus_tag="PXB27_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4365 domain-containing protein"
                     /protein_id="HDL5345411.1"
                     /translation="MLSPTQQMEQFNIAYVLAITANAGFNHAVPVVDNHSVDLAISAE
                     FPTEEGKRSDPEIKLQLKSEGDLHIKNGLVSYKLKKKNYDDLRKNCANPRYLIVCDLP
                     KKTTQWLSHKKKFMTLKRHCYWVSLKGFPATTNRSSVTLTFPENQRFTTDVLIKMIEY
                     ARVGETL"
     gene            complement(1725..2456)
                     /locus_tag="PXB27_000589"
     CDS             complement(1725..2456)
                     /locus_tag="PXB27_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL5345412.1"
                     /translation="MRPLKEIRYDNLLRLIDEAKSTSDLANRTGIAVSYLLQIKNKNA
                     IQNGKPKGIGDKIAAKLEDGMNKPRGWLDQIHSEQSNLINSRIGNEEQKLQEQDLNDF
                     IIIDVLDVSASAGFGSSSELVEVVNQMRYVPEQFYSLFRNMAPRYIRIINLSGDSMYP
                     TFSSGDMLFVDISVNEFTGDGVYVFTYKGHLYVKRLQNTGDQILVISDNKLYEKWSIT
                     EENQDQLFIHARVKVHQSQQLNFIG"
     gene            2583..2807
                     /locus_tag="PXB27_000590"
     CDS             2583..2807
                     /locus_tag="PXB27_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5345413.1"
                     /translation="MQLQEYIEKSKWGEISRLAKAIDVAIPIVSFWVNGKRQVPAKRC
                     PEIEKFTEGKVTCEELRPDVDWAVLRNSGK"
     gene            2856..3617
                     /locus_tag="PXB27_000591"
     CDS             2856..3617
                     /locus_tag="PXB27_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HDL5345414.1"
                     /translation="MKSQSLLPITAQNASGAEVKISSIEIAELCEKQHKNVIVDIRKM
                     LAELKLNSADFSAQYQTKDGRMQPCYFLPKRETMILVSGYRIDLRAKIIDRLDELEKQ
                     HQPSIPKNYPEALRMAAELAEQNQVLLLEKQQTTAENHALKSYFEPGLTPAQFVKGLN
                     GVNSNKINDYLRSRGWLYKDKHCWRVFSHVRDKYLTESFKRIEVDPVERIEMATYKPI
                     LLEKGAVKIFEAYLQGKLPMKADWNGKYYHSKVAV"
     gene            3614..4405
                     /locus_tag="PXB27_000592"
     CDS             3614..4405
                     /locus_tag="PXB27_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5345415.1"
                     /translation="MSMLLMVKAMNCTVGNSARKLVLLKLADNANDDGVCFPSYQFIA
                     DKCEMSKRSAISHIDELIKMGYVSKKSRKNKDGSSSNLYQLHLDAPSEKSALGGENFA
                     PGGSENSAPITSHSINQSINHNTSEQNSDGVDKKSKYRFSDNDMAVAKWIFKRVKQLA
                     PQTKESYLPNWANEVRLMVERDKHTHQEICSLFKFATEHHFWGRNILSVVSLRAKWDR
                     LNLERQSSLRPNNIHSATGNGDIQDTTGFSKGLVVDVGGGTKWEF"
     gene            4393..5028
                     /locus_tag="PXB27_000593"
     CDS             4393..5028
                     /locus_tag="PXB27_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDL5345416.1"
                     /translation="MGILATTTQNQVLPVEIVEQAEVETTKIFNRLKATHLGWRANIK
                     SQAEYDNARREWVRLLIENRLTPKEVERGLKIADTDKNPYLPSFGQFLEWCKSIDYAE
                     LGLPDMDQFLKRLNHFSAFGFTEIDNFKFRSNAEYWLLTDLYERNRKHSWKDETLRNQ
                     AEKALVEMAKRIMSGEQIPPPKPVIEEKKKVPIDPRIQRILDEKKQAGAMQ"
     gene            5025..5597
                     /locus_tag="PXB27_000594"
     CDS             5025..5597
                     /locus_tag="PXB27_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine methyltransferase"
                     /protein_id="HDL5345417.1"
                     /translation="MTDFDKDTYQTPNYVRNWLNHRYAWFHIDGCSNGKNALYSHWIG
                     KAAEGLDEDTLSGQIAEDFLAENLLDVILECVADWGELLRIFVNPPYSNPLPFVQRAA
                     ELKKAGHIVVMLLPADKSTRWYQVIQENASEVIDIIGGRINFINPLTDEEVKGNSKGS
                     MVAVFDPFVEGIVTRQMPLDKIKELGGYEK"
     gene            5667..6695
                     /locus_tag="PXB27_000595"
     CDS             5667..6695
                     /locus_tag="PXB27_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HDL5345418.1"
                     /translation="MAEGLLLTPYFQTEVGIVFYKVPKDVSLAAFGERTLLQPAPTDL
                     ATVKSGKVANFSANSTACKELAEFAKSAKVRSTLNQRDKYSNDLPYTRFVKSIQTCQC
                     RDGKYCHHEKVFVENEKGAVQLCWYHDKMRMDGEIPNEKLEALADENWEKFIAQQIRL
                     TLRKTQSSAIEFADLVLFASIKGLLDELDSNALQQFLGYPPLNNSGVSKESNIGFDDV
                     PNARANLKKWAETLKLKVEPEPLAAFMAKPKLQRFEWRKWLQFVKAQPCMCCGQQADD
                     PHHIIGYGGAMGSKQHDLFTLPLCRIHHDELHRNVGKFEQDYGSQLELLIKFLDRAIG
                     LGALEIDG"
     gene            6688..7047
                     /locus_tag="PXB27_000596"
     CDS             6688..7047
                     /locus_tag="PXB27_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDL5345419.1"
                     /translation="MVELVLPYPPSVNDYWRWVSKTRRVVCKRGKDYQWATFLAAQNK
                     PKFGGQVEIKCDVYFPDNRDRDLDNLGKCILDSLVYSKVIVDDSRHYVKKLTFEDKGN
                     QKGGAVIVRIKERLDVN"
     gene            7037..7396
                     /locus_tag="PXB27_000597"
     CDS             7037..7396
                     /locus_tag="PXB27_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345420.1"
                     /translation="MLIDIKGILRFWGYSANGRLGTEFPCVAAGMKPAVSASNHRILR
                     LTDESIFEIDRCIKQLKEQEPQQYEVLIGRYAARVSDTQIEKVLGISHSTFKRELAQA
                     EMYVLGVVVGLKLALVV"
     gene            7476..8282
                     /locus_tag="PXB27_000598"
     CDS             7476..8282
                     /locus_tag="PXB27_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HDL5345421.1"
                     /translation="MKKLVLVTALSLVLSACGSGGGGGSSASNPNKEATPQSPSKSET
                     KSDTSSQNQVKGALSKVSADLQEQVKALKQINVDGRIIELAQEPIGFIEKDLDNGKKG
                     KAYNLAYSSIGYVLPKDVKTDEYGRVIDERASANDVGIFGLATKFQDLPKSGTAHYSG
                     VSFGANSEGKLTLTADFGDKKVNGSITERKLLSNNQALSSINLLSAPISQTKFAGEEI
                     HFAGIAQANVEGNIVQAPYGGKFMGPKAEEVVGFVADDHNDPYEVFAGQK"
     gene            8446..8793
                     /locus_tag="PXB27_000599"
     CDS             8446..8793
                     /locus_tag="PXB27_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HDL5345422.1"
                     /translation="MPEKNPDIYTQAWNYFAMFVSNHSSFICAVIIAFFTSITKSFLY
                     GKTDTPKRVFAEAILCAVIAGSLRPILIYFNADVSLITPIGAGIGLLGTSAIRQMILK
                     FLNKKAGVDDNVN"
     gene            8783..9379
                     /locus_tag="PXB27_000600"
     CDS             8783..9379
                     /locus_tag="PXB27_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 19 protein"
                     /protein_id="HDL5345423.1"
                     /translation="MLISERKFKQVFPSAIDGIYAEIAKHIEKAGCKTKEQQAMFLAQ
                     CGVECAGYKTFEENLNYSATALLATFPKYFTQHNVVLYARDKRAIANRVYANRMGNGN
                     EQSGDGWKYRGRGLIQITGKANYIEFAKWEGLPSLVTSPDQITDNLALTVQVAVWYWQ
                     SRKLASYGSDILKVTKLINGGTHGLSQRTNYYQRLMVA"
     gene            9380..9730
                     /locus_tag="PXB27_000601"
     CDS             9380..9730
                     /locus_tag="PXB27_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HDL5345424.1"
                     /translation="MSKYIYLVATILILGLCGWIWQQSKQINTLRAENQTQAQTIEQQ
                     QKANQRLADKLEQERQAVEKIRKSANEMRNKVEIVQNEIKSILAQDSCAKADLPNGVA
                     DSIKRLHRQNNHKH"
     gene            9735..9908
                     /locus_tag="PXB27_000602"
     CDS             9735..9908
                     /locus_tag="PXB27_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345425.1"
                     /translation="MYPPAAYLVPCERTEFSGNTYGDTVEYLIKVMGERDLCANQIDR
                     IREWQAQTKQGFK"
     gene            10044..10442
                     /locus_tag="PXB27_000603"
     CDS             10044..10442
                     /locus_tag="PXB27_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345426.1"
                     /translation="MIVKLKLGAVVVLSQVKQATIAYAGSEAYQTAIEQAKKGLLDED
                     KGKPFETVLHFPAVYADEDGQEEICPEQIATTVRSDNVSGEPVAVVLIPDDNVVGELQ
                     SIFIYKTDYAEIFTDNGVKLSAVRTSPAPR"
     gene            10590..11066
                     /locus_tag="PXB27_000604"
     CDS             10590..11066
                     /locus_tag="PXB27_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HDL5345427.1"
                     /translation="MDNLFDLKLNINQIAELTGLHRQTVSQRVAGLNPALGSNSKLKL
                     YALRDLILTGLAEKMSADVDSLSPADRRAWFQSENERLKFEKEIGELIPASEVALEMS
                     ALAKTVVQALETLPDILERDCGLQPKDLIRVQQVTDDVRDQMALHIQEVTTDTENE"
     gene            11066..13177
                     /locus_tag="PXB27_000605"
     CDS             11066..13177
                     /locus_tag="PXB27_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010786315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HDL5345428.1"
                     /translation="MFASAKEIRRDIANAIKAPRRMRVSEAVAEYMRVPLGGGNSVKW
                     DKHTAAYMLEPMDCLNSREYDAVIFVGPARTGKTIGLIDGWITYSIICDPSDFLLVQL
                     TQEKASEHSRKRLDRTFRCSPEIAKRLSPRKNDNNVHDKYFRAGNLLKIGWPSINVLS
                     SSDYKYVALTDYDRWPEDVDGEGDGFSLASKRTTTFMSAGMTLVESSPGKDIVDLKHI
                     PKSTHEAPPTTGILSLYNRGDRRRFYWQCPDCSEYFEPSMANMVGYREDSDFVKASEN
                     ARLQCPHCQTLIAPELKRELNIKGVWLKEGQKIDASGQISGESRKSRIASFWLEGPAA
                     AYQTWAQLTYKLLNAEHEYEMTGSEETLKAVTNTDWGLPYLPRSALEQRRSDELMERR
                     EEVEEKTVPAQCRFIVAAVDVQGGKNRRFVVQMVGYGENGERWLIDRYNISHTLPDSD
                     GVIEKIDPRIPDDWYILISDVLEKRYPLAHNPNHFMPILAMAVDSGGEDGVTDNAYQF
                     WRKCRRDGYAKRVYLVKGDSTKRQKLITKTYPDNTARSDRHSSARGDVPLYLLQTDYL
                     KDRINNALFRDTVGANYIHFPDWLGEWFFNELTYEERGADGKWRKPGKGNNEAFDLFC
                     YAHAIAILRGYERIKWGDENDVPSWAKLPDINPNIIRETPRTAEQAVEIEEKSEKPQP
                     KPTKAKSSWLSGGGRKTGGWL"
     gene            13174..13398
                     /locus_tag="PXB27_000606"
     CDS             13174..13398
                     /locus_tag="PXB27_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345429.1"
                     /translation="MSLYTIDELKQKIRTLDEKIETAQSQVSFNGRSVSYQVAELTKQ
                     RDRYQTMLDEQLAQSGQRTKQHRIKYARFI"
     gene            13398..14897
                     /locus_tag="PXB27_000607"
     CDS             13398..14897
                     /locus_tag="PXB27_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL5345430.1"
                     /translation="MNFLEKTIATLSPKWAANRSRNRYVLNAYEAAQPSRTHKASRES
                     KGANSTVRQSAVSLREQARALDQNHDIVIGILDKMEERVIGSKGIHIEPQPLTLAGEV
                     HEELAGQIRKLWAEWSVKPDVTGLYTRPLLERMLLRTWLRDGEVFVQLVKGKVAGLEH
                     GSQVAFSLEALEPDFVPMQSDEAKNGLVQGVFLNAWRKPTAYQVYLDNPQESNGMYGK
                     VKTVPADNMLHLAFRKRLHQIRGVSMLHGVIVRLADLKEYEESERVAARIAAAMTMYI
                     KKGDAALYDDDSNSDGERLFDIAPGAVIDDLKPGEDIGLINSNRPNVNLETFRNGQLR
                     ATAAGTRSSYSSIARDYNGTYSAQRQELVESFEGYAVLQDAFVAAISRPIYREWLKMA
                     IASQAIKLPPDIDQNSLFNAVYSGPVMPWIDPIKEANAWKERIKGGLATEGQAIRASG
                     NNPAEVKRQRIVEIKENQAEGLKFDTDLTNYGGQDKSFSNQKDEINEDE"
     gene            14909..16870
                     /locus_tag="PXB27_000608"
     CDS             14909..16870
                     /locus_tag="PXB27_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HDL5345431.1"
                     /translation="MKTWFNIQAKANDTAEISIFDEIGFWGVTAQQFAKDLKALGNNL
                     KQINLHIHSPGGDVFDGIAIYNLLKNHPANKTVYIDGLAASMASVIAMAGDEIIMPEN
                     AMMMIHKPWGIQGGDADDMRKYADLLDKVESTLIMAYVAKTGKYETDLAEMLKEETWL
                     TGKECVEQGFADKLAEPLVAMACIQSKKLEDYTKMPDKIKNMLFAPQGNAGANPKHEQ
                     PQTPPQAGENPQNVAKPDAMVALAQRNATIKATFAAFGTQFDGLLAECLADVSMTAEQ
                     AKDKLLAKLGENTTPSVPQNHIHAGNGNIVGDSVKQSLMARAGQDTDKTNARDNAYNA
                     MTLRELARASLVDRGVGIAGYTPMQMVGLAFTHSSSDFGQILIDVAHKSLLKGWETAT
                     ENYEQFTTRGTLTDFRPAKRVGLGEFGYLPEVGEGEEYTYGTIGDEGASVALATYGQL
                     FSITRQAIINDDMHLLTKIPEKMGAAAKATITKLVFALITGNATAQDGKKLFDATHKN
                     SLTGAALNVENIDKAIQLMNGFVNSRGEPLSIEPEFMLLPTSLHTKAKQILGSSSVEG
                     ADINSGIINPIRDIVSPIKSPRLQIADPKSWYLLNKEAIEVSYLDGVDSPYIEQQHGF
                     TVDGVSTKVRIDAGVNVIDYRGVVKVTNA"
     gene            16944..17267
                     /locus_tag="PXB27_000609"
     CDS             16944..17267
                     /locus_tag="PXB27_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HDL5345432.1"
                     /translation="MAKNYIQDGNTVRLTATKAITSGDVIVAEDLIAIAVSDAAKNDA
                     VVGLTTGVFSVKAKQADDIKQGAVLYWSETEGATLTAGSNKRLGIAWKDSGTSSNQVD
                     VKINV"
     gene            17260..17562
                     /locus_tag="PXB27_000610"
     CDS             17260..17562
                     /locus_tag="PXB27_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5345433.1"
                     /translation="MSSPFEQAIAAADQTIQQTMMSEWLIGGKPYPATFDEAPAIFNG
                     LHSTDDRAIHGTERTLTLFRASGYKPRLDDRVSGNGKKYLVKSYHFVDQLIVLQLE"
     gene            17566..18090
                     /locus_tag="PXB27_000611"
     CDS             17566..18090
                     /locus_tag="PXB27_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5345434.1"
                     /translation="MGSQVTGLKELQASLKKIARQTVPKAAAQAIRTVGRQAMNKAVK
                     SVAAEIGVNQKTIKGRAKMTAKPTPSRLQATIKVNRSHMPMIRVLERKSNRLSPTKGS
                     IRVGKHTVQRGFRQRLANGRTHIMYRQGRKRYGIDVAKVPLSQPLTQAFDRELKNYPE
                     QVQAELAKQIAGKL"
     gene            18087..18482
                     /locus_tag="PXB27_000612"
     CDS             18087..18482
                     /locus_tag="PXB27_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5345435.1"
                     /translation="MKIHTKIRKEVLELLQTKLPEIDNFYNGLPTFIDIEEEQLAVSV
                     YLDDISLQEITVCNNQWDAKLNITIHLKSTESAEDELDEWAEKIREIIDDYPEFEHLE
                     SVALSQYQYEQDQNQRTWHMATLIFDVEY"
     gene            18510..19151
                     /locus_tag="PXB27_000613"
     CDS             18510..19151
                     /locus_tag="PXB27_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345436.1"
                     /translation="MAKTTKVQGTKFRISVGREAQKAITAINLNTATLTIASSGYKKG
                     DAIELTGCGQLDGIYPVLSVTTDQVKLCEEVNWTGKDLPANYTKAKAALVQYSEQFCA
                     VKNIEKSDDTLSTEDVTTVCSEGTETEPGEIEFGSIKLSFFHKPTTEMQTRLRKLFYD
                     KSTFAYKLELPDNHGTTYGEGFIEAGNGFSGEVKGKYEGSVSIKPSKRDYLLV"
     gene            19235..19636
                     /locus_tag="PXB27_000614"
     CDS             19235..19636
                     /locus_tag="PXB27_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345437.1"
                     /translation="MTLREKLLANKPKLQPIEINGETYYLREATVGDMNKQIFETRSW
                     LIQQAEQENVELPAEDDETFDEALNRFGEKYRLAQSVAYRLCDENGVLLFNPLDINDL
                     NAIAELDSKVIIDFNQAVSAPKDSASEESSK"
     gene            19681..19911
                     /locus_tag="PXB27_000615"
     CDS             19681..19911
                     /locus_tag="PXB27_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HDL5345438.1"
                     /translation="MPERHFAEYRLFYQEQPFGLWRDDYRTAQLAHLTAMFNRDPKGK
                     APKLAEFMPFFNRTDEVEEIEDDGVADYLAKR"
     gene            19974..20201
                     /locus_tag="PXB27_000616"
     CDS             19974..20201
                     /locus_tag="PXB27_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345439.1"
                     /translation="MFEALDRFMINTIKIAIIVFLVACIFISMPNWWIASILVLFPTA
                     YLLDKYWWRNTEMYRRKMDKLVEEMKNNRQI"
     gene            20255..23821
                     /locus_tag="PXB27_000617"
     CDS             20255..23821
                     /locus_tag="PXB27_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HDL5345440.1"
                     /translation="MSSSNRLTITLDVENSKLLAGFAQSDTVANKFARSFIVNMDQAQ
                     QKARQFADRSTKYLKNIEQAANNLNSSVSKERFWQIANRLQFPTSKVISYANSYTELG
                     NKLKLVTESESQHARAMAAVYDISLKTAQSTQATSSVYQTFAQNAKQLGISQNDVAKL
                     TETVAKSVAISGASSATASNALVQFSQSLLMGKLKAQEFNSLMTQTPSVIQAIAKGLG
                     ITTAELKAMVDNGEMSAEKMIEGLKKAESYVNGQYAKTTTTISGAMQNLSTAAEKWVG
                     EMDSTVGVSQKAVAVLNALNANFDTVAKTVLVAGGAFALFKGYMNVRPFIESRAAIYE
                     NQKAIIAENQARQQAMAIRQQELVGLSQQTALKLEQTLQTYNQNKAELEAIAVKQQKI
                     TKEREEIAVAIQAAATRSERLALSAELQALDQKEITLGQQKIAVERQQIAAKQALKVA
                     YAENAVAQSAMSAGMITATTTTRAMNGVLAAARAELNLLKAAALSNPLMTLAMVATTA
                     GTALWGFFESQKAAREEALRYADSLDSVRVNIEKMTKAQTDAEMVKLSRSIKEQETAL
                     ANLKQRQAELTREIEQGATVYEDAFAGTVRVTMSAEELAKKQEELTLVTADAEKAQKT
                     LNSSLEAQEALKAHIPIANLREEFVKLYPHIDESQIKVDGLNIAIGNFTVKSPEMVIA
                     TNNIANALGGVAGEAMRAAILVANLSSMKLDANGAAIIDPKHLEKIEQIERNNAIGSA
                     KGKDKIALQVKDALIKSGMKEGDAGYERLKAAYEQQFTLQNAPKGKGGKTKAEKEAEK
                     AAKKQQSYQNQVAEMTNRLAGLKANASDIAIFGQVSDYQEVRKLTEDIAINAEKYKGY
                     GEQGVARLKELASQLESAQQQVAISQFAYNGGEKLKAMEFELTLLGKTRQEQELMQYN
                     HELDLEAARLKIGMADENIAKLDQEIVKLKERRAEIQAQAEQARGSFKQGMLDGLNNI
                     EADVSNVAANVANITQNTFDGMADSLTNFVMTGKADFRSFANSILSDLSKMIIKMMLF
                     NAIKQGATMLFGYQGPTTTVNGNAVYGLGTWATGGYTGDGGKYTPAGIVHKGEYVITK
                     EATARLGLDYLNYLNYGKRGFSSGGGVAVPRVPSSSYQPKSAQSNISVQVINNGEPVD
                     AKVSQKQQGDQMQITVELMREIARQEANGMIQNNFRAGGVFA"
     gene            23821..24150
                     /locus_tag="PXB27_000618"
     CDS             23821..24150
                     /locus_tag="PXB27_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5345441.1"
                     /translation="METLKWCVRTDLAVENTPEIMEVKFGDGYTQRSPKGLNNLLRAY
                     TGTIKVKKGEHLAVEAFFAKHRGVSPFYFKDPYTQQNKKVVCSSWPAKMVGLNHWEFS
                     VTFKEVP"
     gene            24150..>24271
                     /locus_tag="PXB27_000619"
     CDS             24150..>24271
                     /locus_tag="PXB27_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDL5345442.1"
                     /translation="MPVSISNQMKLDLAKLEQNAMLDLYEVDLRTLKDKSGNAGS"
BASE COUNT         7564 a         4894 c         5492 g         6321 t
ORIGIN      
        1 gcggtttaac tgcttaataa ataaacaggt aagaagtgca aatggtcatt ttgaattggg
       61 attttgcaga ttttaggcaa aagaaaaccg ccacgagggc ggtttgtcta taagatattt
      121 attgaggtta atttttgtag ccgacctttt ttcttattga tatataggtt ttctccttta
      181 catcgaattt gctgttcggt tttgtgtgct tcagctgcca atttgtactg ctcaggagga
      241 aggtcaaccc taatgtcaac cgttttatct tctattttac aaggtacctg aatataacct
      301 ccatcagcta aattgctgga gtgtaaccct acaactttgc caatgatttc atagtgtggg
      361 agggtatatt ctccttggta atacctagat gcaacctgaa ttactttaat ttctttttga
      421 gaaaatttaa tattaaatgt ttcagaaggc attgattcat cactaatttc acccacttgg
      481 agtttaattt cgatttttct ttgttggtct gaaccactta atccaataat agcttcacaa
      541 agattatgac ttactccttc aggtatcaat tcagcaaaaa tagtcggatt gtgatcataa
      601 tgagtaattt tttctttcag cttgttcagg ctgttaacca aattatgcga tacactccga
      661 gaaaatgaaa ttgcctgaat aatttcattc tgttccttaa tttcaatatt ctcaacagga
      721 taagataaat tgacaatata gcttccaatt tgagtttgcc ctaaacgaac ttgagacata
      781 aactctgcta catctttgcc acctgagtta tgtccaaaag ctgccttttt ctttttagct
      841 gaaagtgcca aggcattaat taaatgtttg gttttttcaa ataattttac accttcgttt
      901 agcggaattg tcccatcttt aacgctttcg ccaatcactc taacagatag cttgtctgta
      961 tattgatttg aaatattaag ctttaacgtt tctacgtttt tttcttcaat tttggcgagc
     1021 ttttcaagtg ctttctgcaa cacttcctct ctatcttcag caattggctt tggtaatgta
     1081 acctctaaaa ttcgatgtcc aagatctttc tgccaaataa ccgcatactc taattcaaac
     1141 gcaatttgcc agcttttggc agttaaatag cgagcaaact gttcgagaaa cagtaacgtg
     1201 tccgttttat tgtttttcat aaagtttctc ctactctagc gtattcaatc attttaatga
     1261 gtacatcagt ggtaaatcgt tgattttctg gaaatgtgag ggtaacacta cttctatttg
     1321 ttgttgccgg aaagcctttt aatgataccc aataacaatg tctttttaaa gtcataaatt
     1381 ttttcttatg tgagagccat tgagtcgttt ttttaggcaa gtcacataca attaagtagc
     1441 gagggttcgc acaatttttc cttaaatcat catagttttt ctttttgagt ttatagctta
     1501 ccagaccatt tttaatatga agatctcctt ctgatttaag ttgtagcttt atttccggat
     1561 cacttctttt cccttcttca gtcggaaatt ctgcagaaat tgcaagatca acactatgat
     1621 tgtctactac tggtaccgca tgattaaagc ctgcattagc agtaatagct aacacataag
     1681 caatattaaa ttgttccatt tgttgtgtag gtgaaagcat atttctatcc aataaaattc
     1741 aactgttggc tttggtgaac ttttactcta gcatggataa atagctgatc ttgattttct
     1801 tcagtgatac tccacttttc atagagtttg ttatctgaaa tcactaatat ttgatcgcct
     1861 gtattttgta atctttttac atacaaatgt cctttgtatg taaatacata aacaccatcg
     1921 ccggtaaact catttacgct aatatcaaca aaaagcatat ctcccgagga aaatgtcggg
     1981 tacatagaat caccacttaa attgataatt ctgatgtatc gaggtgccat attccgaaag
     2041 agggaataaa attgctcagg cacataacgc atttgattta ctacttcaac taattcactg
     2101 cttgagccga agcctgcact agcactgaca tctaatacat caattatgat aaagtcattt
     2161 aaatcttgct cctgcaattt ttgttcttca ttgcctatcc ttgaattgat aaggtttgat
     2221 tgctctgaat gtatttgatc taaccagcct ctaggtttat tcatcccatc ttctaatttg
     2281 gcggcaatct tgtcaccaat gccttttggc ttaccgttct gaatagcatt tttatttttg
     2341 atttgtagta gatagcttac tgcgatacct gttcgattag ctaaatcaga tgtgctttta
     2401 gcctcatcta tcaagcgtaa taaattatcg tatctaattt cttttagggg tctcatatcc
     2461 cacctccata ctatttgctt tattttatta tcatttcgat aacatttaaa tatgatctat
     2521 tttgttgatt ttgatttatc tttaagataa tattcactta tcttttaggt aaggattatg
     2581 aaatgcaatt acaagaatac atagaaaaat ctaagtgggg ggaaatctct cgattagcta
     2641 aagccatcga tgtagcaatt ccaatagttt cattttgggt aaacggcaaa cgccaagtac
     2701 cggcaaagcg ttgcccggaa attgaaaaat ttacagaagg gaaagtaact tgtgaagagc
     2761 tacgccctga tgttgattgg gcagttttac gaaattcagg caaataaaaa accaccgctg
     2821 gaacggtggc taattacaac ctaaggaggt ttattttgaa atcccaatca ttattaccga
     2881 taaccgcaca aaatgcaagc ggtgcggaag tcaaaatcag cagtattgaa attgcagagt
     2941 tatgcgaaaa acaacataag aacgttattg tagatattcg caagatgtta gctgagctga
     3001 aattgaactc ggctgatttt tcagcccagt atcaaaccaa agacggcaga atgcaaccct
     3061 gctactttct tccaaaacga gaaacaatga ttttagtttc cggctatcgc attgatctga
     3121 gagccaaaat cattgaccgc ttagatgagt tagaaaagca acatcagccc tctattccga
     3181 aaaattaccc cgaggctctg cgaatggcag cggagttggc ggaacaaaac caagttttat
     3241 tgttggaaaa acagcagact accgcagaaa accacgcctt gaaatcctac tttgaaccgg
     3301 gtttgacccc tgctcagttt gtaaaagggc tgaatggcgt aaatagtaac aagataaatg
     3361 attatttacg tagtcgaggc tggttataca aagacaagca ttgctggcgg gtgtttagcc
     3421 acgttcggga taaatatctg acggagtcat ttaagagaat tgaagttgat cctgttgaac
     3481 gtattgagat ggccacctac aagccaattt tgcttgagaa aggtgctgtt aagatttttg
     3541 aggcttattt acaaggtaag ttaccaatga aagccgattg gaacggcaag tattatcaca
     3601 gtaaggtggc ggtatgagta tgttattaat ggttaaggcg atgaattgta cggtgggaaa
     3661 ttctgcccgt aaattagtgc tgttaaaact cgctgataac gcaaatgatg atggtgtttg
     3721 ctttcctagt tatcaattta ttgcagataa gtgtgaaatg tctaaacgca gtgctattag
     3781 ccatattgat gaattaatca aaatgggata tgtgagcaaa aaatcacgca aaaacaaaga
     3841 tgggtctagt tcaaatttat atcaattaca ccttgatgcc cctagtgaaa aatctgcact
     3901 agggggtgaa aattttgcac cagggggtag tgaaaattct gcacccataa ccagtcactc
     3961 tattaaccag tcaattaacc ataatacgtc cgaacaaaat tcggacgggg tggataaaaa
     4021 atcaaaatat cgtttttctg ataacgatat ggcagtggca aagtggattt ttaaacgagt
     4081 gaaacaacta gctcctcaga ccaaggagtc ttacttgccg aattgggcca atgaagtccg
     4141 cttgatggta gaacgtgata aacatactca ccaagaaatt tgctcgttgt ttaaatttgc
     4201 gaccgaacac catttttggg gcaggaatat cctaagtgtt gtatctcttc gagctaagtg
     4261 ggatcggcta aatctagaaa ggcaatcatc attacgacca aacaatattc actcagcaac
     4321 aggcaatggt gatattcaag acacaacggg gttttcaaaa ggtcttgttg ttgatgtagg
     4381 aggagggaca aaatgggaat tttagctaca acgactcaaa atcaagtatt acctgttgag
     4441 attgtggaac aagcagaggt ggaaacaacc aagattttca accgcttgaa agcaactcac
     4501 ttaggctggc gagccaatat caagagtcaa gccgaatatg ataatgctcg ccgtgaatgg
     4561 gttcgtttgt tgattgaaaa tcgcttaacg ccaaaagaag ttgaacgagg tttgaaaatt
     4621 gcggataccg ataaaaatcc ctatttaccg agctttggac aattcctcga atggtgtaaa
     4681 tccattgact acgcagaatt aggtttgcct gatatggatc aattcctcaa gcgtttaaat
     4741 catttttccg cttttgggtt tactgaaatc gataatttta agtttcgttc aaacgctgaa
     4801 tattggctct taactgacct ttatgaacga aatcgaaaac atagttggaa agatgaaaca
     4861 ttacgtaacc aagctgaaaa agcgttagta gagatggcaa aacggattat gagcggtgaa
     4921 caaattccac ctcctaagcc agtaatcgaa gagaaaaaga aagtgccaat agatccacgc
     4981 attcagcgaa ttttggacga gaagaaacaa gcaggagcaa tgcaatgaca gattttgata
     5041 aagacactta ccaaacacct aattatgtgc gtaattggct aaatcatcgc tatgcgtggt
     5101 ttcacattga cggttgttct aacggcaaaa acgcattgta tagccactgg attggcaaag
     5161 cggcagaggg cttagacgaa gacacactaa gtggtcagat tgccgaggat ttcttggcag
     5221 agaacttgct cgatgtgatc ttggaatgtg tggcagattg gggcgaatta ctgcgtattt
     5281 tcgtgaatcc gccatacagc aatccactgc cgtttgtaca acgggcggca gagctgaaaa
     5341 aagccggcca tattgtggta atgcttttac cggcagataa atctacccgt tggtatcagg
     5401 ttatccaaga aaacgctagt gaggtgatcg acattatcgg cggacgtatt aatttcatca
     5461 atccgctcac agacgaggaa gtaaaaggca acagcaaagg ctcgatggtg gcggtgttcg
     5521 atccgtttgt ggagggaata gttacccgtc aaatgccgtt ggataagatt aaggagctgg
     5581 gcggctatga aaaataacga aggctgggac ggtttagata aggcgtatca ttgccttagc
     5641 cgcaacaaaa agaaatgggg gcaataatgg cggaaggttt gctacttacc ccttactttc
     5701 aaaccgaagt gggtattgtg ttttacaaag tgcctaaaga tgtttctctt gctgctttcg
     5761 gtgagcggac tttgttgcaa cctgcaccga ccgatttagc aacggttaaa tcaggcaagg
     5821 ttgcaaattt ttcggcaaat tcgaccgctt gtaaagagtt ggcagagttt gctaaatcgg
     5881 caaaagttcg ctcaacgcta aatcagcgtg acaaatacag taatgatttg ccttataccc
     5941 ggtttgtaaa gtcaattcag acgtgccaat gccgagatgg taaatactgc caccacgaaa
     6001 aagtattcgt ggagaatgaa aaaggtgcgg tacagctttg ttggtatcac gacaaaatgc
     6061 gaatggacgg tgaaatcccg aatgaaaaat tagaagcact agcggatgaa aactgggaaa
     6121 aatttatcgc ccaacaaatc cgcttaacct tacgcaaaac gcaatcttct gccattgaat
     6181 ttgcggactt agtgttgttt gcgagtatca aaggtttgct tgatgaatta gacagcaacg
     6241 cattacagca gtttctcggc tatccaccac tgaataatag cggtgtgagc aaagaaagta
     6301 atatcggatt tgatgatgtg ccgaatgctc gagccaacct caaaaagtgg gctgaaacgt
     6361 taaaactgaa agtcgagccg gagccgttgg cggcatttat ggcaaaaccg aaattacagc
     6421 ggttcgagtg gcggaagtgg ttgcagtttg taaaagcaca gccttgtatg tgttgcggac
     6481 aacaagcaga cgacccacat catattatcg gctatggcgg agcgatgggg agcaaacagc
     6541 acgatttatt cactctgccc ctatgccgta ttcatcacga tgaattgcat cgcaatgtgg
     6601 gtaaatttga gcaagattac ggctcgcaat tagagttgtt gattaagttt ttagaccgag
     6661 cgataggttt gggggcgtta gaaattgatg gttgaattgg tgctgcccta tccgccaagt
     6721 gtaaacgact attggcggtg ggtaagcaaa acaagacgag tggtgtgtaa acgaggcaaa
     6781 gactatcaat gggcaacatt tctcgcagca cagaataaac ctaaatttgg cggacaggtt
     6841 gaaattaagt gcgatgttta cttccctgat aaccgagatc gggatttgga taacttaggc
     6901 aaatgtattt tagattcgtt ggtatatagc aaagtgattg ttgatgactc ccggcactat
     6961 gtcaaaaagc tgacatttga ggacaagggc aatcaaaaag gcggtgcggt aattgtaagg
     7021 attaaggaga gactagatgt taattgatat taaaggtatt ttacggtttt ggggctattc
     7081 cgccaacggc agacttggta cggaatttcc ctgtgtggcg gcagggatga aacctgctgt
     7141 atcggcaagc aaccaccgca tattacgttt aactgacgaa tccatttttg aaattgaccg
     7201 ctgtatcaag caacttaaag agcaagagcc tcagcaatat gaggtgctga ttggtcggta
     7261 tgcagcaagg gtatctgata cacaaatcga aaaagtatta ggtatcagtc actccacctt
     7321 taaacgagaa ttggcacagg cggaaatgta tgtgctgggt gtggtagttg ggttaaaatt
     7381 ggcgttggtg gtttaaataa tgtgatctgt atcacaacat taattcaata aatgagataa
     7441 agtatagact ctttttacct atggagattt taccaatgaa aaagttagtt ttagttacag
     7501 cattaagttt agttttatct gcttgcggta gtggtggagg tggtggctca tcagcatcaa
     7561 atcctaataa ggaagctact ccacagtccc catctaaaag tgaaactaag tcagatactt
     7621 catctcaaaa tcaagtaaaa ggtgctttat ctaaagtttc ggcagattta caagagcaag
     7681 taaaagcact taagcaaatt aatgttgatg gtagaatcat tgagttagcc caagagccta
     7741 tcggttttat agaaaaagat ttagataatg gcaaaaaagg aaaagcctat aacttagctt
     7801 attctagtat tggttatgtg ttacctaaag atgtaaaaac ggatgaatat ggacgagtaa
     7861 ttgatgagcg agcttctgct aatgatgtcg gtatatttgg actagcaacc aaattccaag
     7921 atttaccaaa atccggaact gcacactact ctggcgtatc atttggtgca aacagtgaag
     7981 gtaaattaac acttactgct gattttggtg acaagaaagt aaatggctca attactgaaa
     8041 gaaagctatt atctaataac caagcattat cttctattaa cttattatct gcccctatca
     8101 gtcaaaccaa gtttgctggt gaagaaatac attttgccgg cattgctcaa gccaacgtag
     8161 aaggtaacat tgttcaagct ccttatggcg gtaaatttat ggggccaaaa gcggaagaag
     8221 ttgttggttt cgttgccgat gatcataatg acccatatga agtatttgct ggtcaaaaat
     8281 aatttttaat gtatgattga caaggtcgcc ctaaaagtat aggatttatg caatccttgc
     8341 gattagtgca agtgcattac gcaatactat ttttatagcc ctgattggtt cagccagtcg
     8401 gggctttttt gttgccccga aaaagtgggg tggagtatga atacaatgcc tgaaaagaac
     8461 cctgatatat atacgcaggc ttggaattat tttgcaatgt ttgtcagcaa tcatagcagt
     8521 tttatttgtg ctgttatcat tgcttttttt acgtctatta ccaagtcatt tttatacggc
     8581 aaaaccgaca caccaaagcg ggtctttgca gaagcgattc tgtgtgcggt gattgccggt
     8641 tcgttgcgtc cgattttaat ctattttaat gctgatgttt ccttaattac cccgattggt
     8701 gccggtatcg gactactagg gacaagtgct attcgtcaaa tgattttgaa attcctcaat
     8761 aagaaagcag gagttgatga taatgttaat tagcgaaaga aaatttaaac aagtattccc
     8821 cagtgcgatt gacggaattt atgccgaaat tgccaaacac attgaaaaag ccggttgcaa
     8881 gacgaaagag cagcaagcaa tgtttttggc tcaatgtggc gtagagtgtg ctggttataa
     8941 aacctttgaa gagaatttga attattccgc tacggcatta ttagccacat ttccgaaata
     9001 ttttacccaa cacaatgttg tgctttatgc ccgagataag cgtgcgattg ctaatcgggt
     9061 gtatgcaaat cgaatgggta acggcaatga acaaagtggc gatggttgga aatatcgtgg
     9121 gcgtgggctg atccagatta ctggtaaagc gaattatatt gagtttgcga agtgggaggg
     9181 gttgccaagc ttggtgacca gtcctgacca aatcacagat aaccttgctc ttacggtgca
     9241 ggtggcggta tggtattggc aaagtcgcaa attagcaagt tacggtagtg acattctaaa
     9301 agtaaccaag ctgattaacg gcggcactca cgggctatca caacgcacga actattatca
     9361 acgattaatg gtggcataaa tgagtaaata tatttatctc gtagccacca ttttgatttt
     9421 gggcttgtgt ggttggattt ggcagcagtc caagcagatt aataccttaa gagccgaaaa
     9481 ccaaacgcaa gcccaaacca ttgagcagca gcaaaaagcc aatcaacggc ttgctgataa
     9541 gctcgaacaa gagcgacaag cggtcgaaaa aatccgaaaa agtgcaaatg aaatgcgaaa
     9601 caaagtggag attgttcaaa atgagattaa gtcaatcctt gcccaagaca gttgtgctaa
     9661 agctgatttg cctaatggcg ttgctgatag cattaagcgg ttgcacaggc agaacaatca
     9721 caagcactga atatttgtat ccgccggcgg cttaccttgt gccgtgcgag cggactgaat
     9781 ttagcggcaa tacatacggc gatacggttg agtatctgat aaaagtgatg ggagagcgtg
     9841 atttgtgtgc taaccaaatc gaccgtatca gagaatggca agcacaaacc aaacaaggtt
     9901 ttaaatagct tggcggatta agccgctatg acatatggga gcgtaacagc tccctttttt
     9961 attgtaccgc attacttagt tatgcttttg gttatatcaa gaccataact aagcaatgtg
    10021 ttctattaac ataaggatta tttatgattg tgaaattgaa attaggtgcg gtagttgtgc
    10081 tatcacaagt taaacaagcc accatcgcat acgctggatc agaggcatac caaacagcta
    10141 ttgagcaagc caagaaaggt ttacttgatg aagacaaagg taaaccattt gaaactgttc
    10201 ttcacttccc tgctgtgtat gccgatgaag atggacaaga agaaatttgc cctgaacaga
    10261 ttgcaactac agtacgttca gataatgtaa gtggtgagcc tgttgcagtg gtattaatcc
    10321 cagatgataa cgtagttggc gagttacagt ctattttcat ttataagaca gattatgcag
    10381 aaatttttac tgacaatggt gtgaaattaa gtgcagtaag aacaagtcca gctcctcgtt
    10441 aattttgtaa aaggtactcc cgaggggata cccctttcca cggggttgcg gcagcgtggt
    10501 tttcggcaat tttttgagtt tctaggcatc atcatcttcc tatgatgttg ctgtttttta
    10561 ggcggttttg atttttagga tttgagcaaa tggacaactt atttgatcta aaacttaaca
    10621 tcaatcagat cgccgaactc acaggattgc accgccagac ggtatctcag cgagtcgctg
    10681 ggttaaatcc agcactcggt agtaatagca aattaaaact ctatgctctg cgtgatttaa
    10741 tcctcaccgg gttggcggaa aagatgtcgg cagatgtcga tagcctatca cctgctgatc
    10801 gtcgtgcgtg gttccagtcg gaaaacgaaa gactaaaatt tgaaaaagaa attggcgagc
    10861 ttatcccagc aagcgaagtg gctcttgaaa tgtcagcttt agcaaaaacg gtagtacaag
    10921 ccttagaaac cttgcctgat attttagagc gagactgcgg attgcagcct aaagatttga
    10981 tacgagtgca gcaagtaact gacgatgtac gagaccaaat ggcattacat atccaagaag
    11041 ttaccactga cacggagaat gaataatgtt tgctagtgct aaagaaattc gacgtgatat
    11101 agcaaatgcc ataaaagcac ctcggcgaat gagagtgtca gaagcagtag cggaatatat
    11161 gcgtgtacca cttggcggag gcaactcggt caaatgggat aagcacaccg ccgcctatat
    11221 gttagaaccg atggactgcc tcaatagccg agaatacgat gcggtgattt ttgtcggccc
    11281 ggcacgaacg ggtaaaacca tcggcttgat tgacggctgg ataacctact caattatctg
    11341 cgatccgtct gatttcctgt tggtgcaact tacccaagag aaagctagcg aacatagccg
    11401 aaaacggctt gaccgcacat tccgctgttc gcccgaaatt gccaagcggc taagcccacg
    11461 caaaaacgat aacaatgtcc acgacaagta tttcagagcg ggcaacttgc tcaaaatcgg
    11521 ctggccgtct atcaatgtgc tttcttcatc ggactacaaa tatgttgctc tgactgacta
    11581 cgaccgctgg ccggaagatg tggacggaga gggcgacggt ttctcgcttg cctcaaagcg
    11641 gacgaccacc tttatgtcag caggaatgac tttggtggag agttcgcccg gtaaagatat
    11701 tgttgatctc aaacatatcc cgaaaagcac ccacgaggct ccaccgacta ccggtatttt
    11761 aagcctctat aatcgaggtg atcgccgtcg gttttactgg caatgccccg actgctccga
    11821 atacttcgag ccgtcaatgg caaatatggt cggctaccgt gaagacagtg attttgtcaa
    11881 agccagcgaa aacgcacggt tacaatgccc tcactgccaa accctgattg cccccgaact
    11941 caaacgagag ctaaatatca agggcgtatg gctcaaagag gggcaaaaaa ttgatgcaag
    12001 cggtcaaatt agtggcgaaa gtcgcaaatc ccgtattgcc tccttttggc ttgaagggcc
    12061 agcggcagcc taccaaacgt gggcgcagct cacctacaaa ctgctcaatg ccgaacacga
    12121 atatgaaatg actggcagtg aggaaaccct caaagcggta acaaataccg actgggggtt
    12181 accttactta ccacgatctg cattagaaca acgccgttct gatgagttga tggaacgccg
    12241 agaagaagta gaagagaaaa ctgtaccagc tcaatgtcgc tttattgtgg cggcggtgga
    12301 cgtacagggc ggtaaaaacc gccgatttgt ggtgcaaatg gtcggttacg gtgaaaatgg
    12361 cgaacgctgg ttgattgacc gctataacat ctcacatact ttgcccgaca gtgatggcgt
    12421 gattgaaaaa attgaccctc gtattcccga tgattggtac atactgattt cggacgtact
    12481 agaaaaacgc tacccacttg cccacaatcc aaaccacttt atgccgattt tggcgatggc
    12541 ggtggacagt ggcggtgaag atggggtaac cgataacgcc taccaatttt ggcgcaaatg
    12601 ccgccgagat ggctatgcga aacgagtcta tttagtcaaa ggcgacagca ccaaacggca
    12661 aaagctgatt accaaaactt accccgacaa taccgcacga agtgaccgcc actcctccgc
    12721 acggggcgat gtgccgttgt acttgctcca aaccgactac ctcaaagatc ggattaacaa
    12781 cgccctgttc cgtgatacgg tgggggcgaa ctacatccac ttccccgact ggctaggtga
    12841 atggtttttt aacgaattga cctacgaaga acgaggagcg gacggcaaat ggcggaaacc
    12901 gggcaaaggc aataacgaag ccttcgacct gttctgctat gcccacgcca ttgcaatttt
    12961 acgaggctac gaacgaatca aatggggcga tgaaaacgat gtgccaagct gggcaaaact
    13021 gcccgacatc aaccctaata tcattcggga aacgccaaga acggctgaac aagcggtcga
    13081 aattgaagaa aaatccgaaa aaccacaacc taaacccacc aaagcaaaaa gcagttggtt
    13141 aagtggtggc ggtagaaaaa caggaggctg gctatgagcc tttacaccat agacgagctt
    13201 aaacaaaaaa tccgcacgct tgatgaaaaa atcgaaaccg cccaaagcca agtgagcttt
    13261 aatggtcggt cggtttcata tcaagtggcg gaactaacca aacagcgtga tcgctatcaa
    13321 acaatgctgg atgaacaact tgcccaaagc gggcaacgca ccaaacagca tcgaattaaa
    13381 tatgcgaggt ttatttgatg aacttcctcg aaaaaaccat tgccaccctt tccccgaaat
    13441 gggcggcaaa tcgctctcga aatcgctatg tgctaaatgc ctatgaggcg gcacagccta
    13501 gccgaaccca taaggcaagc cgagagagca aaggggcaaa ctctactgtt cgccaaagtg
    13561 cggtcagcct acgggaacaa gcccgagcct tagaccaaaa tcacgatatt gtgatcggca
    13621 ttctcgacaa aatggaagag cgggtgattg gctcaaaagg cattcatatt gaaccgcagc
    13681 cgctgacgtt ggcaggtgaa gtccacgaag aattagctgg gcaaatccga aagctgtggg
    13741 cggaatggtc ggtgaaacct gatgtgacgg gattatacac tcgcccattg ctagaacgaa
    13801 tgctacttcg gacttggcta cgagacggcg aagtctttgt tcagttggtg aaaggcaagg
    13861 tggcagggtt agaacacggc tcacaggttg ctttttccct tgaagcctta gagccggatt
    13921 ttgtgccaat gcaatccgat gaggcaaaaa acggcttggt gcaaggcgtg tttctcaatg
    13981 cgtggcggaa gccgactgct taccaagtct atttagacaa tccgcaagaa agcaacggaa
    14041 tgtacggcaa agtgaaaacc gtgccggcag acaatatgtt gcacctcgcc tttcgcaagc
    14101 ggttacatca aattcgtggt gtaagtatgt tgcacggtgt gattgtgcga ttggcggatc
    14161 tgaaagaata cgaagagagc gaacgagttg ccgctcgcat tgccgccgca atgacgatgt
    14221 acattaaaaa aggcgatgcg gcactgtatg atgacgacag taacagcgat ggcgaacgcc
    14281 tgtttgatat tgcccccggt gcggtgattg atgatttaaa accgggtgaa gacatcggct
    14341 taatcaattc caaccgcccg aatgtgaatt tggaaacttt ccgcaatgga caactgcgag
    14401 ccactgccgc cggtactcgt tcgagctatt ccagcatcgc ccgagactac aacggcactt
    14461 actctgcaca gcgacaagag ctggttgaaa gttttgaagg ttatgcggtg ctacaagatg
    14521 cgtttgtggc ggcaatcagc cgcccgattt atcgggaatg gctcaaaatg gcaatcgcct
    14581 cacaagcgat caaactgccg cctgatattg accaaaactc tctctttaat gcggtttatt
    14641 caggtcctgt aatgccgtgg attgacccaa tcaaagaggc gaatgcgtgg aaagaacgaa
    14701 ttaaaggcgg tttagccacc gaagggcaag caatccgagc cagtggcaat aacccagccg
    14761 aagtcaaacg gcaacgaatt gtcgagatca aagaaaatca agccgaaggg ctaaaatttg
    14821 ataccgatct caccaattac ggtgggcagg ataaatcttt ctccaatcaa aaagatgaaa
    14881 ttaacgaaga tgaataacag gagggtgcgt gaaaacgtgg ttcaacattc aagccaaagc
    14941 gaatgacacc gccgaaatct caatttttga cgaaatcggc ttttggggtg tcaccgccca
    15001 acaatttgct aaagacttaa aagcccttgg caacaattta aaacaaatca atctgcatat
    15061 ccactcgccc ggtggtgatg tgtttgacgg cattgccatt tacaacctgc taaaaaatca
    15121 ccctgctaac aagaccgtct atattgacgg tcttgccgca tcaatggcta gcgtaattgc
    15181 aatggcaggt gatgaaatca ttatgcctga aaacgctatg atgatgatcc acaaaccgtg
    15241 gggcattcaa ggcggtgatg cggatgatat gcgtaaatat gcggatttac tggataaggt
    15301 tgaaagtaca ctgattatgg cgtatgtcgc caaaacaggt aaatacgaaa ccgatttggc
    15361 agaaatgctg aaagaagaaa cttggctcac aggcaaagag tgcgtggagc aaggtttcgc
    15421 cgataagtta gccgaacccc ttgtggcgat ggcttgtatc caatccaaaa aattagagga
    15481 ctacacaaaa atgcctgaca aaatcaaaaa tatgctattc gcaccacaag gcaacgcagg
    15541 tgcaaatcca aaacatgaac aacctcaaac accaccacaa gcgggtgaaa atccgcaaaa
    15601 tgtggcaaaa cctgatgcaa tggtggcatt agcccaacgc aatgcaacga ttaaagcgac
    15661 ctttgccgct ttcggcacac aatttgacgg cttgctggca gaatgtttag ctgatgtgtc
    15721 aatgaccgct gagcaagcca aagacaagct ccttgccaaa ctgggcgaaa acaccacccc
    15781 aagcgtaccg caaaaccaca ttcacgcagg taacggtaac atcgttggcg acagcgtgaa
    15841 gcagtcgtta atggctcgtg ctggtcaaga taccgacaaa accaacgcaa gagataatgc
    15901 ctacaatgca atgaccttgc gtgagttggc tcgtgcctca ttggttgatc gtggcgtggg
    15961 tattgcgggc tacaccccaa tgcagatggt cggtcttgcc tttactcatt cgagctccga
    16021 cttcggtcaa atcttgattg atgtggctca taaatcccta ctcaaaggct gggaaaccgc
    16081 taccgaaaac tacgagcagt ttaccacccg tggcactctt actgacttcc gaccggcaaa
    16141 acgagtcggt ttaggtgagt ttggctacct gccggaagtg ggcgaaggtg aagaatacac
    16201 ctacggcaca atcggtgatg aaggtgcatc tgttgcattg gcgacttacg gacaactgtt
    16261 tagcattacc cgtcaggcga ttatcaatga cgatatgcat cttttaacta aaatccctga
    16321 gaaaatgggt gcagcagcca aagcgactat taccaaactg gtgtttgcct tgattaccgg
    16381 caatgcgaca gcccaagacg gtaaaaagct gtttgatgct acacataaaa acagcttaac
    16441 gggtgcggca ttgaatgtgg aaaacattga caaggcaatc cagttaatga atggctttgt
    16501 aaacagccgt ggtgaaccgc tctctattga gccagaattt atgctgttac caacctcatt
    16561 gcataccaaa gcaaaacaga ttcttggttc gtcaagcgtg gaaggtgcag acatcaacag
    16621 cggtatcatt aacccgatcc gtgatattgt cagcccgatt aaatcgcctc gcttacaaat
    16681 tgcagatccg aaatcatggt acttgctcaa taaagaggcg attgaagtct cttatctgga
    16741 cggggtggat agcccttaca tcgagcaaca gcacggcttt acggtggacg gtgtttcgac
    16801 caaagtccgt atcgatgctg gtgtgaatgt gattgactac cgtggtgtgg tgaaagtgac
    16861 caacgcttaa tttaaacaat ttaaaacctg accgcacttt taacagtgcg gttttttatt
    16921 atccgaaaaa aggaaaaaga gatatggcta aaaactatat tcaagacggc aataccgtgc
    16981 gtttaactgc aaccaaagcg attacttccg gcgatgtcat tgtggcggag gatttaattg
    17041 cgattgcggt ttctgatgcg gcaaaaaatg atgcagtagt tggcttaact accggtgttt
    17101 ttagcgtaaa agctaaacaa gcagatgaca tcaaacaagg tgcagtgttg tactggtctg
    17161 aaacagaggg cgcaactctc accgcaggct caaataaacg acttggcatt gcgtggaaag
    17221 actctggcac atcatctaat caagttgatg tgaaaatcaa tgtctagccc gtttgagcag
    17281 gcaatagctg ccgccgatca aactattcag caaactatga tgagtgagtg gttgattggc
    17341 ggcaagcctt accctgcaac ttttgatgaa gcccccgcta tttttaacgg tttacactca
    17401 accgatgaca gagctattca cggcacggaa cgcacactga ccctatttcg ggcgagtggc
    17461 tacaaaccga gattggacga ccgagtgagc ggaaatggca agaaatacct tgttaaatcc
    17521 tatcactttg tcgatcagtt aatcgtattg caattggagt aaacaatggg ttctcaagtg
    17581 accggtttaa aagaactgca agcctccctg aaaaaaatag ctaggcaaac cgtacctaaa
    17641 gcagccgctc aagcaatccg tactgttggg cggcaagcaa tgaataaggc ggttaaatcg
    17701 gtggcggcag agattggcgt aaatcaaaag acaatcaaag gtagagcaaa aatgacggca
    17761 aagcccacgc cgtcaagatt gcaagccacg attaaggtta atcgatcaca tatgccgatg
    17821 attcgggtac tagagcgcaa gtccaaccgc ctgtcaccca ccaaaggcag tattagagtt
    17881 gggaaacaca ctgtacaacg tggttttcgc caacgactcg ccaatgggcg aacgcacatt
    17941 atgtaccggc aaggtcgtaa gcgttacggc attgatgtag caaaagtgcc gttatcccaa
    18001 ccacttacac aagcatttga tcgagaattg aaaaactatc ccgaacaagt gcaagcggaa
    18061 ttagctaaac aaattgcggg gaaattatga aaatccacac aaaaatccga aaagaagtcc
    18121 ttgaattatt acagactaaa ttgccggaaa ttgataattt ttataacggg ctgccaactt
    18181 ttattgatat tgaggaggaa caacttgcag tatcggttta tctcgatgat atcagtctgc
    18241 aagaaataac tgtttgtaat aatcaatggg acgccaagct caatattaca atccatctga
    18301 aatctaccga gagtgcagaa gatgaattag acgaatgggc ggaaaaaatt cgtgaaatta
    18361 tcgatgatta tcccgaattt gagcatttag agtcagtcgc gctctctcag tatcaatatg
    18421 agcaagacca gaatcagcgt acgtggcaca tggcgacatt aatctttgat gttgagtatt
    18481 aaacaaataa tctctatagg agacttaaaa tggcaaaaac aacaaaagtc caaggcacga
    18541 aatttagaat tagcgttgga cgtgaggctc aaaaagcaat taccgcaatt aacttaaata
    18601 ctgctactct aacgattgca tcctcaggct ataaaaaagg tgatgcaatc gagcttacag
    18661 gttgtggtca attagatggt atctacccgg tgttatcagt aactaccgat caggtaaaat
    18721 tatgcgaaga agtgaactgg acaggtaaag atttaccagc taattacacc aaagccaagg
    18781 cggcactagt gcaatactcg gagcaattct gcgcggtaaa aaacattgaa aaatctgatg
    18841 atactttgag taccgaagat gtaacaacgg tttgttcaga gggcacagaa accgagccgg
    18901 gtgaaattga atttggttcg atcaaactca gtttcttcca caagcctact accgaaatgc
    18961 aaacccgatt gcgcaaactc ttttacgaca aatccacctt tgcctacaaa ctggaactac
    19021 cggataacca tggcacgacc tatggcgaag gctttattga ggccggcaac ggtttctcgg
    19081 gcgaagttaa gggtaagtat gaaggctctg tatctattaa gccaagcaaa cgcgactact
    19141 tattagttta attgcaaaaa aaccataaaa ttcgaccgtt tgtagaaata acaagcggtc
    19201 tttttatatc agaattttac aaaaggaacg aacaatgaca ctgcgtgaga aactccttgc
    19261 taataaacca aaattgcaac ctatcgaaat caacggcgag acctactacc tgcgtgaagc
    19321 tactgttggt gatatgaaca agcagatttt tgaaacccga agctggctca ttcaacaagc
    19381 tgaacaagaa aatgttgaat taccggcaga agatgatgaa acctttgatg aggctctcaa
    19441 ccgttttggc gaaaaatacc gccttgctca atcggttgcc taccgcttat gtgatgaaaa
    19501 cggtgtatta ctgtttaatc ctctcgacat taacgatctg aatgccattg ccgaattaga
    19561 cagtaaagtg attatcgact ttaaccaagc cgtgtccgcc ccaaaagact cagcgagcga
    19621 agaaagttcc aaataaccct ttcgctcgca ctgggtaaaa cacttgaaga aatcgaacaa
    19681 atgcccgaac gccactttgc cgaatatcgg cttttctacc aagaacagcc tttcggctta
    19741 tggcgagacg attaccgcac cgcccaactt gcccacttaa ccgcaatgtt caaccgtgat
    19801 ccgaaaggca aagctccaaa attagcggag tttatgccgt tttttaatcg gacagatgag
    19861 gtggaggaaa tagaagatga tggtgtggca gattatttgg cgaaaaggta aagaaaacag
    19921 cttgaaattc cttgctcttg gatatatatt tcctcaaaga gaaggaggga agtatgtttg
    19981 aagcactaga ccgttttatg attaatacga ttaaaattgc aattattgta tttttggtcg
    20041 cttgcatctt tattagtatg ccgaactggt ggatagcttc tatcttagta cttttcccga
    20101 ctgcctattt gcttgataaa tactggtggc gaaataccga aatgtatcgc cgcaaaatgg
    20161 ataaattagt tgaagagatg aaaaataatc gtcaaatcta aattaacatc aattctagcc
    20221 cgctttatgc gggcttttta tttggggtga aaaagtgagt agctcaaaca gattaactat
    20281 taccttagat gtagaaaatt ctaagttact agctggattc gcacaatctg atacagtagc
    20341 aaataagttt gctcgcagct tcattgtgaa tatggatcag gctcagcaaa aagcacgtca
    20401 atttgctgat agatcgacaa aatatctaaa aaatattgaa caagcagcaa ataatcttaa
    20461 tagctctgta tcaaaagaac gtttttggca aattgcaaat cggttgcaat tcccgacaag
    20521 taaagtgatt agttatgcca atagctacac cgaactcggt aataaactca aattagtcac
    20581 cgaaagtgaa tcccaacacg cccgagcaat ggcggcggtg tatgatattt cgctcaaaac
    20641 cgcccaatct acacaggcaa cctcttcggt ttatcagacc tttgctcaaa atgcaaaaca
    20701 gctcggtatt tctcaaaacg atgtggcaaa actaacggaa acggtggcga aatcagtggc
    20761 gatctctggt gcaagttcgg caaccgcttc aaacgcccta gtgcaattca gccaatcgtt
    20821 gttaatgggt aagctcaaag cccaagaatt taactcattg atgacccaaa caccgtctgt
    20881 tattcaggcg attgccaaag ggttaggaat tactactgcc gaacttaaag caatggtgga
    20941 taacggtgag atgtcggctg aaaaaatgat tgaggggcta aaaaaagcag agagttatgt
    21001 aaacggacag tacgccaaaa caaccactac aattagtgga gcaatgcaaa atctttcaac
    21061 tgcggctgaa aagtgggttg gcgaaatgga tagtaccgtt ggtgtgtcac agaaagccgt
    21121 tgccgttctg aatgcgttaa atgctaattt tgacacggtt gctaaaacgg tacttgtcgc
    21181 cggtggagcc tttgctcttt ttaaaggcta tatgaatgta cgcccattta tcgagtcaag
    21241 agcggctatt tatgaaaatc aaaaagcaat tattgctgaa aatcaagccc gccaacaggc
    21301 aatggcgatt cgtcagcaag agttagtagg actgtctcaa caaaccgctt taaaactgga
    21361 acaaacatta caaacctaca atcaaaataa agccgaatta gaggcaattg cagttaaaca
    21421 gcagaaaatt acaaaagaac gggaagaaat cgcagtggca attcaagcgg ctgcaactcg
    21481 ttcggaaaga ttagcgttat ccgccgaatt acaagcctta gatcaaaagg aaatcacgct
    21541 cggacaacag aaaatagcgg ttgaacgcca gcaaattgca gcaaagcagg ctctcaaagt
    21601 ggcgtatgct gaaaatgcgg ttgctcaaag tgcaatgagt gcaggtatga tcacagcaac
    21661 cacaacaacc agagcaatga atggtgtttt agcggctgca agagctgaat tgaatttact
    21721 gaaagcagca gcactgtcta acccattaat gacactcgca atggtggcta caactgccgg
    21781 tactgccctt tggggatttt ttgaaagtca aaaagcagct cgtgaagaag ccctccgcta
    21841 cgctgacagc ttagactctg ttcgagtcaa tatcgaaaaa atgactaagg ctcaaactga
    21901 tgcagaaatg gttaaattgt ctcgttcgat caaagaacag gaaacagcat tagctaatct
    21961 gaaacaacgc caagctgaac ttacacgaga aatagagcaa ggagctacgg tttatgagga
    22021 tgcgtttgcc ggcactgtgc gtgttacgat gagtgcggaa gagcttgcga aaaaacaaga
    22081 agaattaaca ttagttactg ctgatgctga aaaagctcaa aaaaccttga attctagctt
    22141 agaggctcaa gaagccttaa aagctcatat tccgattgct aacctacgag aggaatttgt
    22201 taaattatat cctcacattg atgaaagcca aatcaaagtt gatggcttaa atattgctat
    22261 cggtaatttt accgtcaaat caccggagat ggttattgct actaataaca ttgctaatgc
    22321 tcttggtggc gttgccggag aggctatgag agcggctatt ctagtcgcta atctgtcaag
    22381 tatgaagctg gatgctaacg gtgctgcaat tatcgatcct aaacatcttg aaaaaatcga
    22441 gcaaattgaa cgtaacaatg cgattggttc ggctaaaggt aaagataaaa tcgcattgca
    22501 agtaaaagac gctctgatta aatcaggtat gaaagagggc gatgccggat acgagcgact
    22561 aaaagcagca tatgagcaac aatttacgtt gcaaaatgcc cctaagggca agggtggaaa
    22621 aaccaaagcc gaaaaagagg cagaaaaagc ggctaaaaag caacaaagtt atcaaaacca
    22681 agtggcggag atgacaaatc gtttggcagg cttaaaagcc aatgcttcag atatcgcgat
    22741 tttcgggcaa gtctccgact accaagaagt gcgtaaactg actgaagata ttgcgatcaa
    22801 tgccgaaaaa tacaaaggct acggtgagca aggtgtcgca aggcttaaag agttagccag
    22861 tcagttagag tccgcccagc aacaagtcgc tatttctcaa tttgcctaca atggcggtga
    22921 gaaactcaaa gcaatggagt ttgaattaac cttgctcggt aaaacccgcc aagagcaaga
    22981 gttaatgcag tacaaccacg aattagatct tgaagcagct cggctcaaaa tcggtatggc
    23041 tgatgaaaat attgcgaagt tagaccagga aatcgttaag ctaaaagaac gtcgagcgga
    23101 aatccaagca caagcagaac aagctcgtgg cagctttaaa caaggtatgt tagatggttt
    23161 gaataatatc gaagctgatg tcagcaatgt agcggcaaat gttgccaata ttactcaaaa
    23221 tacctttgac ggtatggcgg atagcttgac taattttgtg atgacaggta aagcggattt
    23281 ccgtagtttt gctaattcca tattatctga tcttagcaaa atgattatca aaatgatgtt
    23341 atttaatgct atcaaacaag gtgcaactat gttgtttggc taccaaggac cgactacaac
    23401 ggtaaatggt aatgctgttt atgggttagg tacttgggca acaggtggct acaccggcga
    23461 tggtggtaaa tacaccccag cgggtatcgt ccacaaaggg gaatacgtca tcaccaaaga
    23521 ggcaaccgct cgtcttggtt tggattacct aaactatctt aactatggca aacgagggtt
    23581 ttccagcggt ggtggcgttg ctgtgccgag agtgccatcc tcatcttatc aacctaaatc
    23641 agctcaaagt aatatcagtg ttcaggtgat taataacgga gagccggttg atgcaaaagt
    23701 cagccagaaa cagcaaggcg atcaaatgca aattaccgtt gagttaatgc gtgaaattgc
    23761 ccgacaggag gcaaacggta tgatccaaaa caacttcaga gctggaggag tatttgccta
    23821 atggaaacat taaaatggtg tgttcgcacc gatttagcgg ttgaaaatac ccctgaaata
    23881 atggaagtca aattcggcga tggttatacc caacgctcgc ccaaaggctt aaacaattta
    23941 ttacgagcct atacaggcac aatcaaagtg aaaaaggggg aacatttagc ggtggaggca
    24001 ttttttgcca aacatcgtgg tgtttcccct ttttacttta aagatccgta cacacaacaa
    24061 aacaaaaagg tagtgtgtag cagttggccc gcaaaaatgg ttggcttaaa ccattgggaa
    24121 tttagtgtaa cctttaagga agtaccctaa tgcctgttag catatccaat caaatgaaac
    24181 tcgaccttgc caaacttgag caaaatgcaa tgcttgattt gtacgaggtg gacttacgca
    24241 cattaaagga taaaagcggt aatgctggtt c
//
LOCUS       DANLUQ010000018        23770 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.018, whole genome shotgun sequence.
ACCESSION   DANLUQ010000018 DANLUQ010000000
VERSION     DANLUQ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 23770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23770
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.018"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(199..2547)
                     /locus_tag="PXB27_000620"
     CDS             complement(199..2547)
                     /locus_tag="PXB27_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDL5345443.1"
                     /translation="MLISAQFSPLVWAENNDVAVLDEVSVVGSTPSISQGSEVTLLKV
                     SDKIIAGKEFKKRSATLGNALASELGVHSNPFGGGASKPIIRGQEGARIRILQNGSDV
                     IDMSNLSPDHAVVADSLLADQVEILRGSSTLLYASSSPVGIVNVVDKRIPTAIPEKGY
                     EVELNSRFDTAAKEKVGALGATFGIGNHIAVRVEGLSRHSDNYRVPGINLGERLNYVP
                     DTYNKSKVGTLGLSFVGERGYIGASYSKRRDNYGLPGHNHKFDFCTGHIYGNKRDKYA
                     YTYLYPHLIGEENIGSNPHFHCGTNHAEDGTHSHDNPFGHAHDHTHKGPWVDLESKRI
                     DVKAELRQPFKGVDKIKASYADADYYHDEKDAGVLATRYHKQLKKDQDYGKPVNIFKN
                     RGKNTRLEVYHAPLGGLTGVWGVQYQTQKSSMNAPKDREVKFPLVENTNKQFSLFGVE
                     QYMWDSVAVELAGRMEKQKIEIEYDRNEIKRLQEHYRISGGKQVEPDLSPYDETAYAY
                     SGTLNWFFHPDYQLSFTASHNERLPTPMELYYHGQHIATNSFEYGNKDLKKEQSNNVE
                     LGLGYQAERIGYKVSVYYNHFKNYIYNENLFRENQLFMRRYNQAKARFYGVEAEASYR
                     FNDQYQATIFGDMVRGWLTNLPPLKINSDYSVFKDYLPENVEKGKDYLLYRADQNTPR
                     TPPMRLGFRFNAEFTPNWSGDLELIRTFTQRRTSQLEYITEGNTMLNVGLSYSNKWKE
                     LDYKISLNGTNLLNQPVYIHTSYHQFVPQMGRNFMLGMEMKF"
     gene            2732..4189
                     /locus_tag="PXB27_000621"
     CDS             2732..4189
                     /locus_tag="PXB27_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345444.1"
                     /translation="MRSTFKLTLAALAVSIVLSGCKSSQGPSIVADSPPPKEITATDG
                     NDKEKTDNKEKKSAQPNKSDPQPDKPANAKDPVKPDAETNKPSKKDKEYTPPATEKID
                     NSSVQPDKMVPNPIENKENDSNKPNPKESDSDNNDKVTEGNKPNAPTETVNKDKEAEK
                     SKKENKEDSSNNTPNINFDGAKPIDTDSKGSPYRVINLNPRKSVVNASAMQKVDFDSF
                     TDESVENSPFVSSDTEKEYNNTLVFGLGAGDKDSYFKNLNQKGDKHLGIHSGVYEDAN
                     LEGNTKKINYLYVNQPYSSYGALFTDQKDSHLFNVRLTAGRDDVKENGDSYAEYGVFT
                     QSGKEAKWNDNLIGDATYKGQAIARVIRQVNGEQVAGIPQFDGDVTLNLHLSKEWNES
                     KLNGKIDSQSVGVITLKESEIANPTALRSNIGFGEEVIVENKENLVGDYAVDFTGPQL
                     EEAVGVVELENEAETFSENEVIKYNAVFGVSKAAK"
     gene            4253..5665
                     /locus_tag="PXB27_000622"
     CDS             4253..5665
                     /locus_tag="PXB27_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HDL5345445.1"
                     /translation="MKKIALLALFPMMLFARDPNTTQAYIEHTAQPTTTPQRVKIEKS
                     VPIFNETELRANQLLTEKLLIQAIYSRRLANMEKLTAIYRDFTHIDPVLLRYAQGKIA
                     LLKGDYAQAIQHFQTILAKQPKLNTVRIELAIALLYDQQLNQAQAQFEKAKSAEHLPP
                     KVHALIEQYQQHLAKKQEWKFSFSANYVRDGNINRTSKQPNIEQTGYVKQPNLLPQTA
                     HGVHYAFGLQRDFNLFDSHYVSFDNQTQGELYWDNHDYDEITNRTLFGYAYKKSDQTT
                     RLLPFYEKRWKHHRSERWTNGIRLEHAQWFSPHWQLATALEYGKKRYFDLPIYNGSYK
                     LASATLLWLRNPQQFFFVGTDFNQDKTQLKQYSSDTKGIRFGWQREWQALELSSRLTL
                     GYTDRRYKDTAILGGKLNLGRIRHDRVQSANLTLWKRDWHWLGLMPKLQFSYAKHRSN
                     LPSLYAYTDKNINLIIESRF"
     gene            complement(5915..6238)
                     /locus_tag="PXB27_000623"
     CDS             complement(5915..6238)
                     /locus_tag="PXB27_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin"
                     /protein_id="HDL5345446.1"
                     /translation="MQKAYQIYTHSEREKVTEAKLELGKVETRLRNEGKTKEEIAQHP
                     EYLQLKRDLNDKQQTFDNAYGTGSKTKRAVDAVTAALQGLAAKDSGQAAVGLASPYLN
                     EQIKK"
     gene            complement(6207..6734)
                     /locus_tag="PXB27_000624"
     CDS             complement(6207..6734)
                     /locus_tag="PXB27_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HDL5345447.1"
                     /translation="MRDKNKERQQTLERYQELYDQDPELRVIAANSEGDLDLLQETFF
                     VKGELTITDSTPKEKWNWLHMCNLNSYSPSPLSVVEFYIKHGVDVNAQDCYGMTPLHY
                     AMRAKNVDAAIALLNAGADPNIENKDQVIPLSMIGAVPKEIELIEHDISEVKETQELV
                     KGIGEIAEGLSDLHA"
     gene            complement(6736..16380)
                     /locus_tag="PXB27_000625"
     CDS             complement(6736..16380)
                     /locus_tag="PXB27_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HDL5345448.1"
                     /translation="MNKHCFRTIFSKTLQRIVVVSELAKLAGKSESESMTSGAILQKI
                     CKIRPLAFSLFCALGFVTLSDNAMAETLIIQADSTAPKNQQPIILQTANGLPQINIQT
                     PNDKGLSHNKYSQFDVAEKGAILNNSRTNTATQQAGMVQGNPYLARGEAKVILNEVNS
                     SKPSVMKGYVEVAGKKAEVIIANPSGLHCDGCGIINADRATLTTGKPQINQGNLEGFV
                     VEKGKVTVAGKGLDNSRVDYTDILAREAKINAGVWSKKETKVITGKNTVKRSNSDNNL
                     QIIHTNQPLAGEEKPTVAIDVGELGGMYAGKIHLIGTEQGVGVRNAGHIGASADTLTI
                     DSQGRIVNSGTFNAQRQVQLNAQHGIDNQGKIENKQGNIQLNSKADIQNSGSIVARGG
                     NIQKKAETKIQQSGETVAKGNITYTARQIHADKNSLIAAGVDVTDTAQGEVRKLEAQS
                     VAGKTIQLTAPEKTTVQGKNIASGKINVAAAEIDANQSQNNAHTVEYSAKSGDIQANS
                     AQFSANQIKLTTPKWLSTEGSQLTAEVIEAQQANLNARGSVWKQTGEQDFRLKAESIH
                     TDSARFSVGGHFEIDGNRLTNNQGLLSSGKRLGLNLNDAVDSVDGKLVAKGELSIRAP
                     LLNNQQGLILSGEKQAITVNTLKNQSGVITSQTEQTLDITSELQNQGGRISASGVAIN
                     AQSLDNREQGEISSVKELALTVSQQVDNRHGVIQSVGNLTLNSTLLDNQGGVLKSATD
                     ITLNVPTVLNNRISENGSLIEAGGSLTMNSFEVENQGTLARQPQPIQGIIASQFVLNS
                     TKQLNNEKGGIYIGSSARFTLSDSLNNRNGEILSWGDLAIFGGKSLTMTNTEGKVSAN
                     NGLEITAKALSGDGDVQAGDIRLNLQENFDITKDINANRSMIIQTEGDILNRKKLAAN
                     DRLQLTANQITNAQNARISSADTRLTATSTVYNEGLINSRSDNDNAQTVIKAQEIHNI
                     GTGNIYGDKVALGAERILNQDKDGTSATIAARQSLGLAAKVIENNTLVYEANKKSGSL
                     IYSGGEIAIGRQLNEQDTVIGNAGQLHNRSSIIEAEGRIQLNADKVANVNSHYRSELE
                     TTDRQTVNEHFILPRDKHNRGGSDVYDNGHLPTINVNQLRKGYYRKTWSYWQKYDEAN
                     LPVITDISQLNATTVLAKPYAQSCANDESVDCTLLPAGNYKKDNPVWTWFNLTPPAED
                     APEMTPEIIQAMNEEDLANQHLSEAEQEKLQKEQGQGIAPVVPARPIEPVRGSTETEA
                     QYQKRLQTYQAELKAYEAAEAAYQRYVLLKPLIDWEAKYGANIQALDEAIAKHNKANL
                     SQEYPHFWNLWIKERMVKENITKASLPAQILAGGDLTYQSDEFINDKSWVIAGKALNP
                     IGRSRIANLDDADAVRQDWELGRRQFSFTEYHGGTRRRHSRYDENDGPLARLKEVHKD
                     MNIFVELENTSPTAYTDYVDAKAANAVKPEGNQVERGNLPDAPNPPTMAIERIPSPNA
                     AAFEVRSMTVDTRLPNQGLYRINPQADSHVLIETDPDFTDKKRWLSSDYMFNALRHDH
                     NNVHKRLGDGFYEQRLIREQINRLTGRQFLGDYTDFDSQYKALMDAGITFAQKFNLRL
                     GITLSPEQVASLTSDIVWFETQTVRLPSGETVSVLVPKVYALARKGDIDGKGSLISAD
                     ALTINSKEIVNQGTIAGRRLAQFNAERLENSGTLSGGVLAAKVSGDLENIGGKIEADK
                     AILLDIAGNFNHTSTTRTTQIDLDGFKRAETNLDRKALLHVKDENGLLQVQAGNITSA
                     GADIINDGQGQTYLSAKNQLNLTALQVGFDEKMGGGNHYRNEALNDVVISRVQGKGDV
                     VVSGKNLYSEGADLDAKARLALLAENDMVLGSATRSRDYEEYHKTKSGGALAKKTKTT
                     YDNISETEHKGTELTGEDILVKAGHDIQGESLLAVSRSGDIDIIGGNNVTLSSATNQL
                     SEQHIRETKRSGFLNGGEIGFTIGSQKTRQENSLEGAIQSTARNTLGSEGGNIRIQAG
                     NKASVSNVDVLISNEKTAVISGKNGVRVESGKDVINSTDNYEFSQSGLSIALSTPVTD
                     AVQSARQSVQKAKATQNEKLKGVYAVKAAEDAVIAAQNAQKVAETLGNLGNALTENHA
                     AAENPAVKISVSLGTQKQQRESRSQSVTHSKSTLNGGNIALISGAGKVALEGVDTRVK
                     DTLLLDGKLGIESKGVADTYQNNTKNKNHSASVGVFIGLDRDSFGIGFEAGVSGGKGK
                     ENIETETWQNNQLQAGKIVTNSANGKLMLDATNVKANHWEGEVQDFEARSRQDISKYK
                     SEYTEGSVHGTLAVGTTGVNGHVAYNGAKLNTAQVENQTAIDIGKSGMDVKVKKNAHF
                     EGAVMTSQAEKENNRFQAGTLTTGDIENHSELKTRSVAISGGTSGVNPMSALSLLGNK
                     NESERSTTKAAIGENIAITLTEDLNAETTLNHLNRDTQNANQKVTKYDISEVKETQEL
                     VKGIGEIAEKAYQIYTHSEREKVTEAKLELGKAQAQKASKEEIGKLEANLDRLQKEFD
                     KNYGTGSKTKRAVEAVTAALQGLAAKDVGQAAVGLASPYLNAEIKKYTEGDTQANLIA
                     HALLGAVEAAATGNNVLAGAAAGAGSEAAADFIVKTLYKGKSTQDLTEAEKQNVALLS
                     QLASGLASGLIGDSTQSAAVGADIGKRAVENNYLSNWQEQKRSQELKSCHGDARCENQ
                     VRLYWGLVDIGQDVSFVSGAVAGVPENLAESVEGLISMFSSPIETIVAMKELIAREDT
                     LSYIANMTKQEYLAKLDKMQVYYEQAGAVGSFNAGKEFGNMMTDVVTALTGAGALVKT
                     GVGTTAKIVTKSGKELAIPKTRSSASLGTCSFHGDMEVKTDKGYRQISSIKVGDKVLA
                     KNERTGITTYQKVQAHYSNPYDYTVYVEVADSKGKYHTIVSNKIHPFFTQVLSGRVPI
                     SSEGHSYNGEIAKAQWVDAQHLQKGYRLLAESGEWQTVTKVKIKQERLKAYNMTVEKD
                     HTYFIRGTNADNEGVWVHNDCWHALPNDAKQVKNIDGYKAYQFTDPKDGKVVTVVQKD
                     LNRFETPNHQAGVDPHFNRLSQQIDAETGRYLSTDNPVDGLYNRAYLRSETLQKIYAN
                     YEKLPNGNYRNKLTRQEIEQPIDIGHMYGWEHRRLSLAAKELNWSQKQFNDYVNARPE
                     NFRLENISENRSHKNEMPGKDNIKSIIDDMKKFQQIGK"
     gene            complement(16432..18207)
                     /locus_tag="PXB27_000626"
     CDS             complement(16432..18207)
                     /locus_tag="PXB27_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HDL5345449.1"
                     /translation="MKTPQNLILSTVLLSASFVAEANSSSLVQQARIDATQQQIDAAQ
                     QHRQQQYQQNQAEKFQPKADVRLETQINDTLTLSNHELPCYPIHQITLIDYSPDNSLT
                     TSQFQWAFEKAVKSFHLTLPHCFGGEGLAVLMKQIQNEIIGKGYVTTRVVTAEQDLRG
                     GKLVLTVIPGKIRNTLVVDSGKVPRFTKLHALTGFTFAEGDILNVRDIEQSLENLKRV
                     PTAEANIEILPSSSEKAEVGDSDLKISYSQAFPFRLNLGLDDAGSKSTGKLQTSATLS
                     IDNIFSANDLFYTSFTHSMKQKGDDNGRRASKNLTLYYSVPFGYWQLAFSHTHHRYHQ
                     EVFGAFDKSYLYAGESDTDKLTFSYLLYRDAKRKTSLSGSLWPRQSQNYIDGSEIEVQ
                     KRRMAGWEAGFSHKEYFPTATLELSANFKRGTAARGALSAPEEDYGEGTSRPKIITAS
                     ASLTKPFFWGKQPWQWQSSLNAQWNKTPLVPQDRFSIGGRYTIRGFDGELTLSGERGW
                     NWRNELSWQFNPNHAFYWAVDGGRVSGWATENQLGHHLMGTAIGFRGGVKGFSYDVFA
                     GRPVRKPEGFKTSDTVAGFNIGYNF"
     gene            18362..19447
                     /gene="queA"
                     /locus_tag="PXB27_000627"
     CDS             18362..19447
                     /gene="queA"
                     /locus_tag="PXB27_000627"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDL5345450.1"
                     /translation="MLVSDFHFNLPDELIARYPTAERSASRLLHLNGETGEFADQQFV
                     DLYDHINEGDLLIFNNTRVIPARLYGRKPSGGKVEALVERVLDEHRCLAHVKASKAPK
                     EGAELIFGEDKLGEGKGFTATMVARHDTLFELVFNEEQPLFELLQQAGHMPLPPYIDR
                     PDEDADQERYQTVYSKVLGAVAAPTAGLHFDNAMLEKLKAKGVDTAFVTLHVGAGTFQ
                     PVRVETIEDHKMHAEYAEVSQEVVDKILATKAKGKRVIAIGTTSVRSVESAAQAAEKD
                     GKLIAPFFSDTAIFLYPGKTFKVIDALVTNFHLPESTLIMLVSAFAGYRNTMNAYAHA
                     VETKYRFFSYGDAMFINKNPNALSDVP"
     gene            complement(19474..20012)
                     /locus_tag="PXB27_000628"
                     /pseudo
     CDS             complement(19474..20012)
                     /locus_tag="PXB27_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015701733.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(20025..22307)
                     /locus_tag="PXB27_000629"
     CDS             complement(20025..22307)
                     /locus_tag="PXB27_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HDL5345451.1"
                     /translation="MTLDRFCIALILFHLPILWLPKAFLFVGIVIGTLVLILSVYLRS
                     KLTAVLGLLVCIGYIQIIQVAKNAEKTTASKTPVQFEISKILKQTDYQTAIATLNTGE
                     KIYLNWQSKQPLLLNQFYQAELALRPISARSNIGNFDRQRWYFANHINMTATVRKVET
                     LAKTDYSLRTNWLNKVKAETDALPTQGFLLALAFGERAWLKTEHWGQFQQTATAHLIA
                     ISGLHIGLAMLFGVYFAKGVQWLCLKSELTALQAVGFSLYFARIVSFITAFGYSYLAG
                     FAVPTARALLAIAFVLLCQVLRRHYTPWQYWGRIVALLILIDPLSLLSDSFWLSILAV
                     ASLILWYQYFPLSRFLNDEYRKKITKFNRLWLSLLHLQIGIWLIFSPVQLYFFEGISA
                     FALLANLIIVPLYSFLLVPLILFTLLTDNLFSTWLLADYLAQFSLWLLDPFSNAWFAL
                     SHWQQWQLLSLNLLVLVLLYCKLYRLSYKKWLSAVAVSLLFNLSFYLPKLFNQTQTEW
                     IMFDVGQGLAMAFIYQQNKAVIYDTGSSWQANDGSRNSMAKMEILPYLKRNGIEVEAI
                     FLSHDDNDHSGGVVELLQAYPKARLISSSKVTYHNINPEPCIAGTSWQFGEWQLLAIY
                     PSHIAERAKNQDSCVILAKNDRLKILLTGDSGVEQERQFAHQLGKIDFLQVGHHGSKS
                     STGETLLSITQPAFAVISAGRWNPWKLPNKQIIERLNRRHINVLNTVETGMIRVKFYP
                     SSIEIEQARGAFSPWYKQFY"
     gene            complement(22449..23663)
                     /gene="sstT"
                     /locus_tag="PXB27_000630"
     CDS             complement(22449..23663)
                     /gene="sstT"
                     /locus_tag="PXB27_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HDL5345452.1"
                     /translation="MSQSLSSKILGGNLVLRIAIGLVLGVLLAFINKEWATSVGVLGQ
                     FFVKSLRAIAPILVFVLVLAAIANKEVGSDSKLKPILVLYVLGTFFAALTAVVLSFMF
                     PTTLELTASPDGLTPPQGIGEILKTVIFNLVDNPLVALSNANFIGILAWSIGLGIAFR
                     HGSASSKAFLNDLANAVSFVVKVVIAFAPIGVFGLVAETVAINGIDAFEDYARLLVVL
                     VGAMLFVALVLNPLLVYWKIRRNPYPLTFTCLRESGVTAFFTRSSAANIPVNMNLAKR
                     LGVRDEIASVAIPLGASINMAGAAITVTVLTLAAAYTQGIQPDFATALLLSVVASICA
                     CGASGVAGGSLLLIPLACSLFNIPNDIAAQVIGVGFVIGVIQDSVETALNSSTDVLFT
                     AAVSYAEDQKSA"
BASE COUNT         6428 a         5498 c         4684 g         7160 t
ORIGIN      
        1 taataaaggg ataaagttaa ttgtcttatt taacaacaaa caggaaattt tggtaagctc
       61 cgaaaataac aagaaaggaa aaacagcact attttatcca aattaccaat acctgagttt
      121 taaacaagcg gttattttta gccgtttttt tgcaaaattt gaagaaaaaa taaccgcttg
      181 tcttgttatg tcgagaactt aaaatttcat ctccattccc aacataaaat ttcgtcccat
      241 ttgtggtaca aattggtgat acgaagtgtg gatatacacc ggctgattca gtaagtttgt
      301 gccgtttaga ctgattttgt aatccagctc tttccactta ttgctatacg acaacccgac
      361 attcaacatc gtattacctt cggtaatata ttccaactgg ctcgtacggc gttgggtaaa
      421 ggtgcggatc agctctaaat cccccgacca atttggggta aattccgcat taaagcgaaa
      481 acctaaacgc attggcggtg tacgaggggt gttttggtca gcccgatata gaagataatc
      541 tttccctttt tccacatttt ccggcagata atctttgaac acactataat cgctatttat
      601 cttcaatggt ggtaaatttg ttagccagcc tcgtaccata tcgccgaaaa tcgtggcttg
      661 gtattggtcg ttaaagcggt agcttgcctc tgcttctacc ccgtaaaaac gggcttttgc
      721 ctgattataa cggcgcataa atagctgatt ttcacggaaa agattttcat tgtagatgta
      781 gtttttaaag tgattgtaat aaacactcac tttatagcca atacgttcag cttgatagcc
      841 caagcccaac tccacattat ttgactgctc ttttttcaag tctttattgc cgtattcaaa
      901 cgaattagta gcaatatgct gcccgtgata atacagctcc attggcgttg gcaagcgttc
      961 attgtgcgag gcagtaaacg aaagctgata gtcagggtgg aagaaccagt taagcgtgcc
     1021 ggaataggca taagccgttt catcataggg tgaaagatct ggctcaacct gtttgccgcc
     1081 actgatacgg tagtgttctt gtaaccgttt gatttcattg cgatcatact caatctcaat
     1141 cttctgcttt tccattcgtc cggcaagctc aacagcaacg ctatcccaca tatactgctc
     1201 aaccccaaac aggctgaact gtttattggt attttcaacc agcgggaatt tcacttcgcg
     1261 atctttcggc gcattcatac tgcttttttg cgtttgatac tgaacacccc atacaccggt
     1321 taagccacct agcggtgcgt gatagacctc caagcgggta tttttgcccc gatttttgaa
     1381 aatattcacc ggcttgccgt aatcctgatc ttttttcagc tgtttatggt aacgggtggc
     1441 aagcacgcct gcatcttttt catcgtggta ataatcggca tcagcatagc ttgccttaat
     1501 tttatctacg cccttaaacg gctggcgtag ctcggctttc acatcaatcc gttttgattc
     1561 taaatccacc cacgggcctt tgtgagtgtg atcgtgggcg tgtccgaacg ggttatcgtg
     1621 gctgtgtgtg ccgtcctcgg cgtggttggt tccacagtgg aagtgcggat tgctcccaat
     1681 attttcctca ccaatcaaat gcggatagag ataggtataa gcgtatttat cccgcttatt
     1741 accgtagata tgaccggtac agaaatcaaa tttatggtta tgccccggca agccataatt
     1801 atcccgccgt ttgctgtatg aggcaccaat atagcctcgt tcaccgacaa aggataagcc
     1861 caacgtgccg actttggatt tattgtaagt gtccggcaca tagtttaagc gctcgcctaa
     1921 gttgatcccc ggcacacggt agttatcact atgacgactc agcccttcga ctcgcaccgc
     1981 aatgtggtta ccgatgccaa aggttgcccc taatgcgccg actttctctt tggcggcggt
     2041 atcaaaacgg ctattgagtt caacctcata tcccttttca ggaatagcgg tcggaatacg
     2101 tttatccacc acatttacaa ttcctaccgg cgaactgctg gcataaagca aggtgctgga
     2161 accacgcaag atttcgacct gatcggcaag cagtgaatca gcaacaacgg cgtgatcagg
     2221 agataggttc gacatatcaa tcacgtctga accgttttgc aaaatgcgaa ttctcgcccc
     2281 ttcctgccca cgaataatcg gcttactcgc tccgccacca aacgggttac tatgcacgcc
     2341 taattctgac gccaacgcat tacccagcgt tgccgaacgc tttttaaatt ccttacctgc
     2401 aataatttta tcactgactt ttagcaaggt aacttcactc ccctgtgata tacttggagt
     2461 gctgccaacg accgaaactt catctaacac cgctacatcg ttattttccg cccaaacaag
     2521 cgggctaaat tgagccgaaa tcagcaatgc caatcctgtt ttcttaaaca caatctttct
     2581 ccttaaaaaa attaaggata ataaggtact gcaaaatatg agtctttaca ataataacca
     2641 taaaaaaatt atatttttta aattttccct attgccaaac aggaaatata tccattaaaa
     2701 ttttatggat ttaatctgac ggagacaaaa tatgcgttct acattcaaac ttactcttgc
     2761 agctttagct gtttctattg ttttaagtgg ctgtaaatct agccaaggtc catctattgt
     2821 tgcagacagc cctccaccta aggaaataac agctactgat ggcaacgata aagagaaaac
     2881 cgataacaaa gagaaaaagt ctgcgcaacc aaataaatcc gatcctcaac cggataaacc
     2941 tgctaacgca aaagacccag taaaacctga tgcggagaca aataaaccaa gtaaaaaaga
     3001 taaagaatat acaccacctg ctactgaaaa aatcgataac tcatcagtac agccggataa
     3061 aatggtgcct aatcctatcg agaataagga aaacgacagt aataaaccta accccaaaga
     3121 aagcgatagc gataataatg acaaagtcac tgaaggcaat aaaccaaatg ccccaacaga
     3181 aacagtaaat aaagataaag aagctgagaa aagtaaaaag gagaataaag aggactcaag
     3241 caataatact cctaacatta attttgacgg ggcaaagcct atagatacag atagtaaagg
     3301 tagcccgtac cgagtaataa acctaaatcc gagaaaaagc gtagtaaatg caagtgctat
     3361 gcaaaaagta gatttcgact cttttacgga tgaatctgtt gaaaattcac catttgtcag
     3421 tagcgatact gaaaaagaat acaataatac acttgttttt gggcttggtg ccggagataa
     3481 agattcttat tttaagaact taaatcaaaa aggggataaa catttaggta ttcatagtgg
     3541 cgtgtatgaa gatgcgaatt tagaaggtaa tactaaaaaa attaactatc tctatgtaaa
     3601 tcaaccttat tcgagctatg gtgctttatt tacggaccaa aaagacagcc atttattcaa
     3661 cgttcgtctg accgctggta gagatgatgt aaaagaaaat ggagatagtt atgccgaata
     3721 tggtgtattc acacaatcag gaaaagaagc taaatggaat gataacctga ttggtgatgc
     3781 cacttacaaa ggtcaagcta ttgctcgcgt tattcgtcag gtaaatgggg aacaagtcgc
     3841 aggtattcct caatttgatg gtgatgttac attaaattta cacctttcca aagaatggaa
     3901 tgaaagtaaa ctaaacggca aaattgatag ccaaagtgtt ggggtaatta cacttaaaga
     3961 aagtgaaata gctaacccta ccgcactccg ctcaaatatt ggttttggag aagaagtcat
     4021 agttgagaat aaagagaatc ttgtgggtga ttacgctgtc gattttaccg ggcctcagct
     4081 tgaagaggcg gtaggcgttg ttgaattaga aaacgaagcc gagacattct ctgaaaatga
     4141 agtcattaaa tacaatgcgg tatttggcgt aagcaaagcg gcgaaatagt attttttgta
     4201 tcttacaaag ggctgttaag cccttttatt tttattattt taaggataaa aaatgaaaaa
     4261 aattgcctta ctggcacttt tcccaatgat gttatttgcc cgagatccta atactacaca
     4321 ggcttatatt gagcatactg cacagccaac tacaaccccc caaagggtca agatcgaaaa
     4381 atcagtgcct atttttaacg aaactgaatt acgtgctaat caattattga ccgaaaagct
     4441 acttatccaa gctatttata gtcgtaggct tgctaacatg gaaaaattaa ctgcgattta
     4501 tcgggatttt acccacattg acccggtttt actgcgttat gcacaaggta aaattgccct
     4561 actgaaaggc gattacgcac aggcaattca gcacttccaa accattcttg ccaaacaacc
     4621 caaactaaac actgtaagaa ttgagctggc gattgctcta ttgtacgatc agcaacttaa
     4681 tcaagcccaa gcacaatttg aaaaagccaa aagtgccgaa catctaccgc ctaaagttca
     4741 cgcattaatt gagcaatatc agcaacatct ggcaaaaaaa caggaatgga aatttagttt
     4801 ttcagccaat tatgtgcgag acggcaacat taaccgaacc tcgaaacagc cgaacattga
     4861 gcaaaccggc tatgtaaaac aacctaattt gttgccccaa accgcacatg gtgttcacta
     4921 tgcgtttgga ttacagagag atttcaacct gttcgactca cactatgtga gttttgacaa
     4981 ccaaacccaa ggtgaacttt attgggataa ccacgattat gatgaaatta ccaatcgcac
     5041 cttgtttgga tacgcttaca aaaaatccga ccaaaccacc cgcttgttac cgttttatga
     5101 aaaacgctgg aaacatcacc gttcggaacg ctggacaaac ggaattcgac ttgaacacgc
     5161 acaatggttt tcaccgcatt ggcaactcgc caccgcattg gaatacggca aaaaacgtta
     5221 ttttgatttg ccgatttata acggcagcta caaacttgcc tctgccacat tgctttggtt
     5281 acgcaatcca caacaatttt tctttgtcgg cactgatttc aatcaagaca aaactcagtt
     5341 aaaacaatat agctctgata ctaaaggcat acgctttggc tggcaacgag aatggcaagc
     5401 cctcgagctt tccagtcgcc tcacgcttgg ctatactgac agacgttaca aagacaccgc
     5461 tatattggga ggaaaactta atcttggcag aatccgccat gaccgtgtgc aaagtgctaa
     5521 cctcacgctt tggaaacgag attggcactg gctcggcctg atgccgaagc tgcaatttag
     5581 ttacgccaaa caccgtagca acctgccgag cctctatgcc tacaccgata aaaacatcaa
     5641 tttaattatt gaaagtcggt tctagctcca taaaaacaac aagcggttaa aattcctaga
     5701 aattttgcaa attgcaaaaa atgtagaaaa tttaaccgct tgtatctata ctgcagcttc
     5761 ttttccttca gtataaatta atatacaccg ttgcaacaat aatggctgct ccctgtcatc
     5821 accttgaact gaaaattgct caaggtggat atgactatcg cagccgcttc caccgcaccc
     5881 agtaatgtat gggcaagcag gttggcttgg gtatctactt tttaatttgt tcgttgagat
     5941 atggcgaagc taagcctact gctgcttgtc ctgaatcttt cgccgctaaa ccttgtaagg
     6001 ctgccgtcac cgcatccacc gcacgcttgg ttttgctgcc cgtgccgtag gcgttatcaa
     6061 aagtctgctg tttatcgttt aaatcccgtt ttaattggag atattcagga tgttgagcga
     6121 tttcttcttt tgtttttccc tcatttctca aacgggtttc cactttgccc aattccagct
     6181 tcgcttccgt cactttttca cgctcactat gcgtgtaaat ctgataagcc ttctgcaatc
     6241 tcgcctatgc ccttcaccag ctcctgtgtt tctttcacct cactaatatc gtgttctatt
     6301 aactctatct ctttaggaac tgctccaatc atagacaatg gaataacttg atctttattt
     6361 tcaatattcg gatctgcccc cgcatttaat aatgctatcg ctgcatcaac attctttgcc
     6421 ctcattgcat aatgtaaagg ggtcattccg tagcaatctt gggcattaac atctacccca
     6481 tgtttaatgt aaaattcgac tacagaaaga ggagatggtg aataactatt taagttacac
     6541 atatgtagcc agttccattt ttcttttggt gtgctatccg taatagtcag ttcacctttt
     6601 acgaaaaatg tttcttgtaa aagatcgaga tcgccttctg agtttgctgc aataactctt
     6661 aattcaggat cttgatcata taattcttga taacgttcta atgtctgctg acgttcttta
     6721 ttcttatctc tcattttact ttcctatttg ttggaatttt ttcatgtcat cgattattga
     6781 tttaatatta tctttccccg gcatctcatt cttatgactc cgattttcag atatattttc
     6841 taatctgaaa ttctccggtc tcgcattaac ataatcatta aattgttttt gactccaatt
     6901 taattctttt gccgctaatg ataatcggcg atgttcccag ccatacatat gacctatatc
     6961 aataggctgt tcaatttctt gacgggttaa tttattgcga taattaccat ttggcaattt
     7021 ttcataatta gcataaattt tctgtaacgt ttccgagcgt aaataagctc ggttatatag
     7081 cccatctacc ggattatctg tcgataaata gcgtcccgtt tcggcatcaa tttgctgaga
     7141 caaacggtta aaatgtggat ctaccccggc ttgatggttt ggcgtttcaa aacgattgag
     7201 atctttttga actactgtca ccactttccc gtctttcggg tctgtgaact gataagcttt
     7261 atagccatca atatttttta cctgtttggc atcattcggt aaagcgtgcc aacaatcatt
     7321 atgcacccat accccttcat tatcggcatt tgtacctcta ataaagtaag tatgatcttt
     7381 ctctaccgtc atattgtagg cttttaatct ttcctgtttg atttttacct tagtgacagt
     7441 ctgccactca ccactttcag caagtaagcg atagcctttt tgcaaatgtt gagcatcaac
     7501 ccactgagct tttgctatct ctccgttgta agaatgcccc tccgaagaaa tcggcactct
     7561 gcccgataat acttgcgtaa agaacgggtg gattttgttt gacacaatgg tgtggtattt
     7621 tcctttgctg tctgcgactt ctacatacac cgtataatcg tatggattac tgtaatgagc
     7681 ttggactttc tgataggtgg ttataccggt gcgttcattt ttcgccagca ccttatcgcc
     7741 cactttaatg ctggaaatct gtcgataacc tttatcagtt ttgacttcca tatcaccgtg
     7801 gaatgagcaa gtgcctaaac tagcgctcga tctggtctta ggaatcgcaa gttctttacc
     7861 tgatttggtg actattttcg ccgttgtccc tacacctgtt ttaacaagag caccagcacc
     7921 ggttaatgct gtcaccacat cagtcatcat attgccaaac tcttttccgg cattaaaaga
     7981 acctacagcc cctgcctgtt catagtaaac ctgcatttta tcgagtttag ctaaatactc
     8041 ctgtttagtc atattagcaa tataggagag cgtatcttct cttgcgatca attctttcat
     8101 cgccacaata gtttctattg ggcttgagaa catggagatc aatccttcaa cgctctcagc
     8161 gagattctca ggaacaccag caacggcgcc tgatacaaaa ctaacatctt gcccaatatc
     8221 aactaatccc caatataatc gaacttggtt ttcacagcgg gcatcaccat ggcaggattt
     8281 taattcttgt gagcgtttct gctcctgcca gttcgagagg tagttattct ccactgccct
     8341 tttcccaata tccgccccaa ccgctgccga ttgtgtacta tcccctatca aacctgaagc
     8401 cagtccgctt gcaagctggc ttagcaacgc cacattctgt ttctcggctt cggttaagtc
     8461 ttgcgtggat ttgcccttgt agagggtttt aactataaag tccgctgccg cttcgctccc
     8521 tgcgcctgca gcagcccctg ccagtacatt gttccctgtc gcagccgctt ccaccgcacc
     8581 cagtaaggcg tgggcaatca ggttggcttg ggtatcccct tcggtgtact ttttgatttc
     8641 tgcatttaag tatggtgagg ctaaacccac tgccgcttgt cccacgtctt tcgccgctaa
     8701 accttgcaag gctgccgtca ccgcttccac cgctcgtttg gttttactgc ccgtgccgta
     8761 gtttttatca aactcttttt gcaagcggtc taaattggct tctaatttac caatctcttc
     8821 cttgctcgct ttttgagctt gtgctttgcc caattccagc ttcgcttccg tcactttttc
     8881 acgctcacta tgcgtgtaaa tctgataagc cttttctgca atctcgccta tgcctttcac
     8941 cagctcttgt gtttctttca cctcactgat atcgtatttc gtcacttttt gattggcgtt
     9001 ttgcgtatct cggttgagat gattaagcgt agtttccgca tttaggtctt cggtgagggt
     9061 gatagcgata ttttctccaa tcgctgcctt tgttgtgctt cgctcgcttt cgtttttatt
     9121 gcccagtaag ctcagtgccg acatcgggtt caccccactt gtccctccgc taatcgcgac
     9181 acttcgggtt ttcaattcac tgtggttttc aatatcgccg gtggtcagcg tgccggcttg
     9241 gaagcggttg ttttctttct ccgcttggct agtcattacc gctccctcaa aatgggcgtt
     9301 ctttttcact ttaacgtcca ttccactctt gcctatgtcg attgcagttt ggttttccac
     9361 ctgagcagtg ttcaacttcg ctccattata tgccacatga ccatttactc ctgttgtccc
     9421 taccgccaat gtgccatgta cgctgccttc tgtgtattcg cttttatatt tgcttatatc
     9481 ttgtcgactt cttgcctcaa aatcttgtac ctcaccttcc caatgatttg ctttgacatt
     9541 ggttgcatcc agcatgagtt taccatttgc agaatttgta acaattttac ccgcttgcag
     9601 ttggttgttc tgccacgttt cagtttcgat attctccttg cctttacctc cactcactcc
     9661 tgcctcaaaa ccaataccaa aactatctct atctaaaccg ataaatacgc ccacactcgc
     9721 actgtgattc ttattcttag tgttgttttg ataagtatcc gcaacacctt tggattcaat
     9781 acccagctta ccgtcaagca gcagggtgtc tttcactcgg gtatccaccc cttccagcgc
     9841 aactttgccc gctcccgaaa tcaaggcaat attgccgccg ttcagggtgc ttttgctgtg
     9901 ggtcacgctt tgactacggc tttctctttg ctgtttttgc gtgccgagac tgactgaaat
     9961 cttcaccgcc ggattttctg ccgcagcatg gttttctgtg agtgcattgc caagattgcc
    10021 cagcgtttct gccactttct gagcattttg tgctgcaatc acggcatcct ccgccgcttt
    10081 taccgcatat acccctttga gtttctcgtt ttgtgttgcc ttcgcttttt gcaccgattg
    10141 ccgtgccgat tgcaccgcat ccgttaccgg tgtactcaac gcaatgctta agcccgactg
    10201 gctaaattca taattatcgg tgctgttaat aacatctttc cctgattcaa cccgtacccc
    10261 gtttttgcct gaaatcacgg cggttttttc gttggaaatc agcacatcga cattgctcac
    10321 gctcgcttta ttgccggctt gaatccgaat attgccccct tcactgccca atgtattgcg
    10381 tgccgtgctt tggattgccc cctctaagct gttctcttgg cgtgtttttt ggctaccgat
    10441 agtaaagccg atttcaccac cgttcaagaa accgctgcgt tttgtctcac gaatatgctg
    10501 ttctgaaagt tggtttgttg ccgaactgag cgttacatta ttaccgccga taatatcaat
    10561 atcgccactg cgtgagacgg caagaagact ttcaccttga atatcgtgac cggctttcac
    10621 taaaatgtct tcaccggtta gttcagtgcc tttgtgttcg gtttcagaaa tattgtcata
    10681 agtcgttttt gttttcttcg ctagcgcacc accacttttt gttttgtgat actcttcata
    10741 atcgcgagaa cgtgttgcgc tacccaacac catatcattt tcagccagta acgctaaacg
    10801 cgctttggca tccaaatccg ctccttcact gtaaaggttt ttccctgaaa ccaccacatc
    10861 gcctttgcct tgcacccgtg aaatgaccac atcattcaag gcttcattac gatagtgatt
    10921 gccaccgccc attttttcat caaaaccaac ctgtaaagcg gtcaaattca actgattttt
    10981 tgcagataag taggtctgcc cctgtccgtc attgatgatg tctgcgcctg ctgaggtgat
    11041 gttgcctgcc tgtacttgca gcagtccatt ttcatcttta acgtgcagta gggctttgcg
    11101 gtcaaggttg gtttcagcgc gtttgaaacc gtctaggtca atctgtgtgg ttcgagtggt
    11161 ggaggtatgg ttaaaattgc ccgcaatatc cagcaaaatc gctttatccg cttcaatctt
    11221 gccgccgata ttctctagat caccgctcac tttggcagca agcacgccac cactgagtgt
    11281 gccactattc tctaaccgtt cggcattaaa ctgtgctaaa cggcggcctg ctatcgtgcc
    11341 ttggtttaca atctctttac tgttgatggt aagcgcatcg gctgaaatca ggctgccttt
    11401 gccgtcaata tcacctttgc gtgcaagggc atacactttc ggcactaata ccgataccgt
    11461 ttcaccgctt ggcaagcgaa ccgtttgggt ttcaaaccaa acaatatcgc tggtcaagct
    11521 tgccacttgc tccggcgata aggtaatgcc taaccgaaga ttgaatttct gcgcaaaggt
    11581 gatgccggca tccatcaggg ctttatattg gctatcaaaa tccgtatagt cgcccaaaaa
    11641 ttgtctgccg gttaagcggt tgatttgctc tcgaattaag cgctgttcat aaaaaccatc
    11701 gcccaaacgt ttatgcacat tgttatggtc gtggcgtagg gcattgaaca tataatcact
    11761 gcttaaccag cgttttttat cggtgaaatc cggatcggtt tcgatcagaa catggctatc
    11821 cgcttgaggg ttaatgcgat aaagcccttg gtttggcaaa cgggtatcga ccgtcattga
    11881 gcgaacctca aatgcggctg cattcgggct tggtatgcgt tcaatcgcca ttgttggcgg
    11941 attaggggca tcgggtagat taccccgttc aacctgattg ccttccggct tcacggcatt
    12001 tgctgcttta gcgtccacat aatctgtata agcggtcggt gaagtatttt ccagctcgac
    12061 aaaaatattc atatccttat ggacttcttt taaacgagcg agaggaccat cattttcatc
    12121 gtagcgggag tggcgacgtc ttgtgcctcc gtggtactca gtaaaagaga attggcgcct
    12181 tcccagttcc caatcttggc gaacagcatc tgcatcatca aggttggcaa ttctactgcg
    12241 accaatcggg tttaacgctt tgcctgcaat aacccaactt ttatcgttga taaattcatc
    12301 gctttgataa gtgagatcgc cacctgccaa gatctgtgcc ggtaagctgg ctttggtgat
    12361 attctctttt accatacgct ctttaatcca caaattccag aaatgcgggt attcttggct
    12421 taaattcgct ttattgtgct ttgcaatcgc ttcatctaat gcttgaatat tggcaccata
    12481 ttttgcttcc caatcaatta acggttttaa tagcacataa cgctgataag ccgcttctgc
    12541 cgcttcatag gctttcagtt ccgcttgata tgtttgtagt cgtttttggt attgggcttc
    12601 cgtttccgta gagcctctaa caggttcaat cggtcttgcc ggaaccaccg gtgcaatgcc
    12661 ttgcccttgt tccttctgta atttttcctg ctctgcctca cttaaatgct gattcgctaa
    12721 atcttcttca ttcattgctt gaataatttc aggcgtcatt tccggtgcat cttcagctgg
    12781 tggtgttaaa ttaaaccacg tccatactgg gttatctttt ttataattac ctgcaggtaa
    12841 tagagtacaa tctacacttt catcattcgc acagctttga gcatagggtt ttgccaatac
    12901 ggtcgtggca ttcagctggc taatatcggt aattaccggc aagtttgctt catcatactt
    12961 ttgccaatag ctccacgttt tgcgatagta gcctttgcgg agttgattca cattaattgt
    13021 cggcaaatgc ccgttatcat atacatcact tccacctcga ttatgcttat ctcttggcaa
    13081 aataaaatgt tcgttcacag tttggcgatc agtggtctcc aattccgaac gataatgact
    13141 atttacattc gctaccttat ccgcattcaa ctgaatacgc ccttcagctt caataatact
    13201 gctgcggtta tgtagctgcc ctgcgttgcc aataacggta tcttgctcat ttaattgacg
    13261 tccaatcgct atctctcctc cgctatatat taatgaaccg cttttcttat tcgcttcata
    13321 aactagggta ttattttcaa ttactttagc cgctaaaccg agagattgac gagcagcaat
    13381 agtggcagag gtcccgtctt tatcttgatt tagtatcctc tccgcaccta acgccacctt
    13441 atcgccataa atattccctg tgccgatatt gtggatttct tgagctttaa tcacagtttg
    13501 agcgttatcg ttgtccgagc ggctattgat taagccttcg ttatagacgg ttgaagttgc
    13561 agtcaaacgg gtatctgccg agcttattcg ggcattttgt gcattggtta tttggtttgc
    13621 cgtgagctgc aagcggtcat ttgcggctaa ttttttgcga ttgagaatat cgccctcagt
    13681 ctgaataatc atcgacctat tcgcattgat atctttggta atatcaaagt tctcttgcaa
    13741 attcaggcga atatcgcctg cttgtacatc accatcaccg gataacgctt tggcagtgat
    13801 ctccaaacca ttatttgcag aaacttttcc ctcggtattc gtcattgtga gtgattttcc
    13861 gccgaaaata gccagatcgc cccaacttaa aatttcccca ttacggttat ttaagctgtc
    13921 gctgagggta aatcgtgctg atgagccgat ataaataccg cctttttcat tattgagctg
    13981 ttttgtgctg ttcagtacaa actgcgatgc aataatcccc tgtattggct gaggttggcg
    14041 tgccaaggta ccttgatttt ccacctcgaa actgttcatc gtaagtgagc cacctgcttc
    14101 aataagtgag ccattttccg agatccgatt atttaagaca gtcggtacat tgagcgtgat
    14161 atctgtggcc gatttcagta ccccaccttg gttatccaaa agcgtgctat ttagggtgag
    14221 atttcccaca ctttgaataa caccgtgccg attatctact tgttgggaaa ccgtgagcgc
    14281 taactctttt actgaactaa tttctccctg ctcacggtta tctaaggatt gagcgttaat
    14341 cgcaacaccg ctggcggaaa ttcgcccgcc ttgattttgc agttcgctag ttatatcaag
    14401 ggtttgctcc gtttgagagg tgatcacccc cgattgattt tttaacgtat tgaccgtaat
    14461 cgcctgtttt tcgccactca aaatcagacc ttgctgatta ttcagcaatg gagcacgaat
    14521 agataactcg ccttttgcaa ccaatttgcc atcgactgaa tccacagcat catttaaatt
    14581 tagccctaaa cgcttgccac tacttaatag cccttggtta tttgttaaac gattaccgtc
    14641 aatctcaaaa tgtccaccca ctgaaaagcg agccgagtca gtatggatac tctctgcttt
    14701 taggcgaaaa tcctgctcac cagtctgctt ccaaaccgag ccacgagcat ttaaattcgc
    14761 ttgttgggcc tcaatcacct ctgcggttag ctggcttcct tcagtggaaa gccattttgg
    14821 ggtggtgagc ttgatctgat ttgcactaaa ctgcgcacta ttcgcctgaa tatcgcccga
    14881 ttttgcactg tattcaacgg tatgggcatt attttggctc tgattggcat caatctcagc
    14941 tgccgccacg ttaattttcc ccgaagcgat attttttccc tgcacagtgg ttttttctgg
    15001 ggcagtgagt tgaatagttt tccctgccac agattgagct tccagcttcc gtacttcacc
    15061 ttgtgcagtg tcggttacgt ccaccccagc ggcaatcagt gagtttttgt cggcgtgaat
    15121 ttggcgggcg gtgtaggtga tattgccttt tgcgaccgtt tcgccacttt gctggatttt
    15181 agtttcggct tttttctgaa tattgccacc cctcgccacg atggaaccgc tattttgaat
    15241 atcggctttc gagttaagct gaatattgcc ctgtttattt tcaattttgc cttggttgtc
    15301 aatcccgtgt tgggcattga gttgcacttg tctttgagcg ttgaacgtgc cgctgtttac
    15361 aatccgccct tgactgtcaa tggtgagcgt atcggcactt gcaccgatat gcccagcgtt
    15421 acgcacgccc acgccctgtt ccgtaccaat caggtggatt ttgccggcat acattccgcc
    15481 cagctcaccc acatcaatcg caactgtggg cttttcttcc cctgcaagcg gttgatttgt
    15541 atgaataatt tgcaaattgt tgtccgaatt tgaccgcttg acggtgtttt tgccggtaat
    15601 gactttagtc tcttttttcg accagacacc ggcgttgatt ttcgcttctc gggcgaggat
    15661 atcggtgtaa tccactcggc tgttgtccag ccctttgcca gcaaccgtta ccttgccttt
    15721 ttccactaca aaaccttcta aattgccttg atttatctgt ggttttccgg tggtcagcgt
    15781 ggctcggtcg gcgttgataa taccgcagcc gtcacagtgc aagccgctcg ggttggcgat
    15841 aatcacttcc gcttttttgc cagccacttc cacataacct ttcattaccg aaggcttact
    15901 ggaattgact tcatttaaaa tcaccttcgc ttcgccacgg gcaagatacg ggttgccttg
    15961 caccataccg gcttgttggg tggcggtgtt ggtgcggctg ttgttgagaa ttgccccctt
    16021 ctccgctacg tcaaattggc tgtatttatt gtgggaaagc cctttatcgt tcggggtttg
    16081 aatattgatt tgtggcagcc cgttggcggt ttgcaaaata atcggttgct gatttttcgg
    16141 ggcagtgcta tcggcttgaa taatcaaagt ttccgccata gcgttgtctg ataaggtaac
    16201 gaatcccaac gcacagaaca agctgaacgc aagcggtcga attttgcaaa ttttttgtaa
    16261 aattgcaccg cttgtcatac tttcactttc tgatttacct gctaattttg ctaattcaga
    16321 taccaccaca atacgttgta aggtcttgct gaaaatagtg cgaaaacaat gcttattcat
    16381 aataattcct taatagcaca atatacttaa tacaatatgc ccaatcattc attaaaaatt
    16441 gtaaccaata ttaaaaccgg caacggtgtc tgaggtttta aacccttccg gttttcgcac
    16501 cggtctaccg gcaaacacgt cataactaaa tcctttaact ccaccccgaa acccaatggc
    16561 tgttcccatt aaatgatgtc cgagctgatt ttccgttgcc caaccggaca ctcgaccacc
    16621 atctaccgcc caataaaaag cgtggttcgg attaaattgc cagctcaact cattacgcca
    16681 gttccagccc cgctcaccgg aaagagtcag ttcaccgtca aatccacgta tggtgtaacg
    16741 cccgccaatc gaaaaacggt cttgcggaac cagaggggtt ttattccatt gggcatttag
    16801 gctactttgc cactgccatg gctgcttgcc ccaaaaaaac ggtttcgtca ggcttgcact
    16861 tgccgtgatg attttagggc gagaagtccc ttcgccataa tcttcctcgg gagcagataa
    16921 tgcccctcgt gctgccgtac ctcgtttgaa attagccgaa agctcaagcg tagcggtcgg
    16981 gaaatattct ttatgcgaaa aacccgcttc ccaacctgcc attcggcgtt tttgtacttc
    17041 aatttctgag ccgtcaatat agttttgcga ctggcgtggc caaagtgaac cggacaagct
    17101 cgttttacgt ttggcatcac gataaagcaa ataagaaaag gttaatttat cggtatcact
    17161 ttcaccggcg tagagatagc tcttgtcaaa ggcaccaaat acttcttgat gataacgatg
    17221 atgagtgtgt gaaaatgcta actgccaata gccaaacggc acggaatagt aaagcgtgag
    17281 atttttactg gcacgtctgc cgttatcatc acctttttgt ttcatcgaat gggtaaaaga
    17341 ggtatagaac aggtcatttg ccgaaaaaat attatctata gacagagtgg cggaggtttg
    17401 taatttgcct gtggatttcg agccggcatc gtccagccca agatttaagc gaaatggaaa
    17461 cgcttgcgaa tagctgattt ttaaatcact gtctcccacc tccgcttttt cgcttgagct
    17521 gggtaaaatc tcaatatttg cctccgccgt cggcacccgt tttagatttt ccagagactg
    17581 ctcaatatct cgcacattca gaatatcgcc ttcggcaaaa gtaaaaccgg ttaaggcgtg
    17641 cagcttggta aaacgcggta ctttgccgct atctaccact aaagtattgc gtattttgcc
    17701 cggaatcacg gttaaaacta attttccgcc acgtaaatct tgctccgcag taaccactcg
    17761 tgtagtgaca taacctttgc caataatttc attttggatt tgcttcatca acacagctaa
    17821 cccttcacca ccaaagcagt gaggtaatgt caaatgaaat gattttactg ctttttcaaa
    17881 cgcccattgg aattggctgg tagtcagaga attgtcagga gaatagtcaa tgagagtgat
    17941 ttgatgaatg gggtaacaag gcaattcgtg attggaaaga gtaagtgtat cattgatttg
    18001 tgtctcgagc cgtacatcag ctttcggctg gaatttttct gcttgatttt gttggtattg
    18061 ctgttggcga tgttgctggg cagcatcaat ttgttgttga gttgcatcaa tgcgtgcttg
    18121 ttgtacaagg cttgaagaat ttgcttcagc aacaaaagaa gcacttaaca ataccgtgct
    18181 taaaatcaga ttttgtgggg ttttcataag atcaaataag taagacattc aacgttttta
    18241 gtctataaaa atttatgttg ttttgcaaac tccgttttat ggagctttat acatatctga
    18301 cgactttggt tttttatgct attatctccg ccatttttat actatagaga atagaataac
    18361 cgtgttagtt tcagattttc atttcaactt acccgatgag ctgattgctc gctacccgac
    18421 tgccgaacgc tctgccagcc gtttattaca cttaaatggc gaaacaggcg aatttgccga
    18481 tcaacaattt gtggatttat acgatcatat caacgaaggc gatctattga tcttcaataa
    18541 cactcgagtg atcccggctc gcctttacgg tcgaaaacct agtggcggca aagtagaggc
    18601 attagtggaa cgggtgctag atgaacaccg ctgcttggct cacgttaaag cctcaaaagc
    18661 accgaaagaa ggggctgaat tgatctttgg ggaagataag ctaggtgaag gcaaaggctt
    18721 tacggcgaca atggtagctc gccacgatac tctgtttgaa ttggtgttta acgaagagca
    18781 accgttattc gaattgctac agcaagctgg tcatatgcct cttccacctt atatcgaccg
    18841 ccccgatgaa gatgctgacc aagagcgtta tcaaaccgtt tatagcaaag tgttaggtgc
    18901 agttgccgcc cctaccgccg gtttgcattt tgacaatgca atgcttgaaa aactaaaagc
    18961 aaaaggggtg gatactgcct tcgtaacatt gcacgttggt gccggcactt tccagccggt
    19021 acgggtagaa accattgaag atcataaaat gcacgccgaa tatgccgaag tgagccaaga
    19081 agtagtcgat aaaattctcg caacgaaagc caaaggaaaa agagtgattg caattggtac
    19141 gacatcagta cgttcggttg aaagtgccgc ccaagcggct gaaaaagatg ggaaattgat
    19201 tgctccgttc ttttctgaca ccgctatttt cctttacccg ggaaaaacct ttaaagtcat
    19261 tgatgcgtta gtcaccaatt tccatttacc ggaatccacg ctaattatgt tagtctcagc
    19321 atttgccggc tatagaaata cgatgaatgc ctacgcccac gcagtagaaa ctaaataccg
    19381 ttttttcagc tatggcgatg cgatgtttat caataaaaac ccaaatgcgt tgagtgatgt
    19441 tccttaattt atgagggtta taagggaata acattatgaa caaaaacaat cttccaacca
    19501 ttttaaggga ttgttttcaa tatattcaat aatttggaag tacgatgttt cattacgaat
    19561 aatgtgttca taataattcc tttgccaaat ttcagaaata tcggtatttt gacgaaccca
    19621 tttagttacg ccaattttaa aaccacgcac aatagagccg attgttcgtg atgttccccg
    19681 taagggcgaa acatttttcg cccttataca tggggcaaat gatgatttgc ccctacagtt
    19741 tcgacgattt caataatgcc atgtaaatga ttaggcataa tcacaaagct atgcaagctt
    19801 acatttggaa aatgatcggg tatatcttcc caacattgac gtacaatatt ccctatctta
    19861 ttaagcctca tttcatcatc aacaatctca ccgagaaaat ggtgtttatt tttacaacaa
    19921 atagtaatga aatagaatcc ttgtgatcgg taatcataat gctttagacg aattgaacgc
    19981 ctcctatgaa tctccggctg atatttctgc atttttctta ttccttaata aaactgctta
    20041 taccaagggg aaaatgcccc ccttgcctgc tcaatttcaa tagaacttgg gtaaaatttt
    20101 actcttatca ttcccgtttc tacggtattt aatacattaa tatgtcttct gtttaatctc
    20161 tcaataatct gtttattcgg taatttccat gggttccatc ttccagcaga aatcactgca
    20221 aatgctggct gggtaatcga taataaagtt tcgccagtgc tgcttttgct accgtgatgt
    20281 ccaacctgta gaaaatcaat tttgccgagc tgatgggcaa attggcgttc ttgttccacg
    20341 ccagaatccc ctgtcagtaa tatcttcaag cggtcgtttt tggctaaaat aacgcaagag
    20401 tcttggtttt tagctcgttc tgcaatgtgt gacgggtaaa ttgccaataa ttgccattcg
    20461 ccaaattgcc acgatgttcc ggcaatacaa ggctcgggat ttatattgtg atacgtcacc
    20521 ttgcttgaac taattaacct tgcttttggg tatgcttgca ataactccac taccccacct
    20581 gaatgatcat tatcatcgtg acttagaaaa atcgcctcta cctcaatgcc atttcgcttt
    20641 aaatacggca agatttccat ttttgccatt gaattgcgac taccatcatt tgcttgccaa
    20701 ctggagccgg tatcgtaaat taccgcttta ttttgttgat aaatgaacgc cattgccagc
    20761 ccttgcccaa catcaaacat aatccattct gtttgggttt gattaaagag cttagggaga
    20821 taaaaactca gattgaacaa tagggatacc gctactgcac ttaaccactt tttataagat
    20881 agccgatata atttgcaata cagcaatact aaaaccaata aattcagtga aagtaactgc
    20941 cactgttgcc aatggcttaa tgcaaaccac gcattagaaa atggatctaa cagccatagg
    21001 ctaaattgag caagatagtc cgccaacagc cacgttgaaa acagattgtc ggtcaagagc
    21061 gtaaataaaa taagcggtac aagcaaaaag ctatacaacg gcacaataat caggttggca
    21121 agcagtgcaa aagcagagat gccctcaaaa aagtagagtt gaaccggtga gaaaatcagc
    21181 caaatgccga tttgtaagtg caaaagcgat aaccacaagc ggttaaattt tgtgattttt
    21241 ttacgatatt catcgtttaa aaatcggcta agaggaaaat attgatacca taaaattaaa
    21301 cttgctaccg ctaaaattga gagccaaaaa ctatcagaaa gcaggctcaa tgggtcaatt
    21361 aaaatcagta acgccactat tctcccccaa tattgccaag gggtatagtg tcgccgcaga
    21421 acttggcaaa gcaagacaaa cgcaatcgcc agcaacgctc tggcggtcgg cacggcaaaa
    21481 ccggctaaat agctgtaacc aaatgcggtg atgaagctaa ctattcttgc aaaataaagt
    21541 gaaaatccga ccgcttgtaa tgcggtaagc tcgcttttca agcaaagcca ttgcacgcct
    21601 ttggcaaagt aaacaccaaa caacattgcc agcccgatat gcaagcctga aatggcaatt
    21661 agatgggcag tagcggtttg ttggaattgc ccccagtgtt ctgttttcag ccacgccctt
    21721 tcaccaaaag ccagagccaa taaaaagcct tgcgtaggca aagcgtccgt ttcagctttc
    21781 actttattca gccaatttgt tcgcaagcta taatcagttt tggctaatgt ttccaccttg
    21841 cgaaccgttg ctgtcatatt gatatgattg gcaaaatacc aacgctgtcg gtcaaaatta
    21901 ccgatattgc tacgagctga aataggacga agagccagtt cggcttgata gaattgatta
    21961 agtaacaacg gctgtttcga ttgccagttc aagtagatct tctcgcctgt atttagtgtc
    22021 gcaatcgctg tttgataatc ggtttgtttt aagattttac tgatttcaaa ttgaacaggc
    22081 gttttactag cagtcgtttt ttccgcattt tttgcaactt gaatgatctg aatatagcca
    22141 atacaaacca ataagccgag tacagccgtt aattttgagc gtagataaac actcaatatc
    22201 agcactaagg ttcctattac tatacccaca aatagaaacg ctttaggcaa ccataaaata
    22261 ggcagatgaa atagaatcag ggcaatacaa aaacggtcta aagtcataat tcggcttact
    22321 ttttaaagct gagtatgccg tttgtgctta atattttcag aataaatttt cagattttcg
    22381 acacagttcg caaaaatact tgaaaaaata aaggcagact ttttagcctg ccttataaag
    22441 aagagatatt atgctgattt ttgatcttca gcgtaactta ctgctgcagt gaataatacg
    22501 tctgttgatg aatttaatgc ggtttcaacc gagtcttgaa tcacaccaat cacaaaaccc
    22561 accccaatta cttgagcagc aatatcgttt ggaatattaa ataaactaca ggctaatgga
    22621 atcaataata atgaaccgcc tgccacacct gatgcgccac aagcacagat tgatgcaact
    22681 acgcttaata gtagtgctgt tgcgaaatca ggttgaatac cttgtgtata agctgccgct
    22741 agggttaata cggttacagt aatcgctgca cctgccatat taatacttgc acctaatgga
    22801 atagctacag aagcgatctc atcacgaacg cccaaacgct tggctaaatt catattcacc
    22861 ggaatgttgg ctgctgaact gcgggtaaag aatgccgtta caccactctc tcgtaaacag
    22921 gtaaatgtaa gtgggtatgg gttgcggcga attttccaat acactaataa tggatttagt
    22981 actaatgcca caaataacat tgcgccaact aagactacta ataaacgtgc gtaatcttca
    23041 aacgcatcaa taccattgat ggcaacagtt tcagctacta aaccaaatac cccaataggc
    23101 gcaaatgcaa tcacaacttt cacaacaaac gaaactgcgt tagctaaatc atttaagaat
    23161 gcttttgaac ttgccgaacc atggcggaat gcaatcccta aaccgataga ccacgcaaga
    23221 ataccgataa agtttgcatt acttaatgcg actaatggat tatcaactaa gttaaaaata
    23281 acggttttta gaatttcacc aatgccttgt ggcggggtta aaccgtctgg gcttgcagta
    23341 agctctaatg tagttgggaa cataaagctt aaaacaacag ccgttaatgc cgcaaaaaat
    23401 gtacctaata cataaagcac cagaatcggc tttaatttgc tatctgaacc aacttcttta
    23461 tttgcaatcg cagccaaaac taatacaaat actaaaatag gcgcaatggc acgtagtgat
    23521 ttcacaaaaa attgacctaa tacaccaacg ctggtagccc attctttatt aataaatgcc
    23581 agcaataccc ctagcactaa accaatggcg atacgtaata ctaaattacc gccaagaatt
    23641 tttgatgata atgattgact cataaccctt cacttctgta agacaacaaa aaataaggat
    23701 aaataaaatt tatccgcctg aaaaaatagg tttataagat tagcggaaaa taaacaaata
    23761 tggaataaac
//
LOCUS       DANLUQ010000019        23437 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.019, whole genome shotgun sequence.
ACCESSION   DANLUQ010000019 DANLUQ010000000
VERSION     DANLUQ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 23437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23437
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.019"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            34..1509
                     /gene="mepM"
                     /locus_tag="PXB27_000631"
     CDS             34..1509
                     /gene="mepM"
                     /locus_tag="PXB27_000631"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HDL5345453.1"
                     /translation="MAVFLLAVFSLFVDIVLALKDQSSINNTSSQEDIIAIDKQEKYQ
                     SINTNLAENNTLHQEQHSSFINYVTDLLTQSALVIEHDDEIVDDQTATSYEDELVGQD
                     DEVEEGIETVENQVPGEKLPEEAEKALEDLLDVADQALRIQNQFSYTVARGDKLKDVL
                     EQSGLTASVAKNLIKQHSALAQLEPGQQFYWVLDNDGELEYMNWLVSEKEERVYERKE
                     NGKFSFQRFEKKGEWKLDVVRGTIQNSFAVSLKAVGLSDRQINQLAVGLQSQIATNKL
                     KKGDRFAILIKREYIDGKVTDLGNVEGIHVISSKKSYYAIQADNGRYYSRYGETLSGG
                     FARHPLLFAARVTSSFNPKRRHPITGRISPHKGVDYGVPIGTPIIAPSDGVVEHVAYQ
                     ARGAGRYIRIKHGHITTVYMHLSKTLVKPGQSVKKGERIALSGNTGGSTGPHLHYEFH
                     INGRPVNPITVKLPGSNSGMGNKERQAFLNKAKNVESRLKM"
     gene            1519..2202
                     /gene="dsbC"
                     /locus_tag="PXB27_000632"
     CDS             1519..2202
                     /gene="dsbC"
                     /locus_tag="PXB27_000632"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HDL5345454.1"
                     /translation="MKKNLITLGLMCLSTITFASDAKLKSQLEKIGATDVKISDSPLT
                     GFKMATSNEGVVYISQDGRYVIQGKVFELKNGKAIDITNQGLLAELNSYTNEMIVYPA
                     KNEKHVITVFMDITCHYCHLLHTKIKEYNDLGITIRYLAFPRGGMNTQTAKQMEAIWT
                     SADKVKALNDAEDGKLPTQIKTPDIVKKHYQLGIKFGVTGTPNMVTSEGELIGGYVEP
                     KELAKMLSE"
     gene            2287..4008
                     /gene="recJ"
                     /locus_tag="PXB27_000633"
     CDS             2287..4008
                     /gene="recJ"
                     /locus_tag="PXB27_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDL5345455.1"
                     /translation="MEKIIKRRPLLTTEIVSTNPLLNRIYQSRGIKNTQELEHNLKNL
                     HRPQQLANIEQAVDLLLQAFREKSRIIIVGDFDADGATSTSLALIALRQLGFDKVDYL
                     IPDRFSQGYGLSVAVAEMVLAKGADLVLTVDNGISSTEGIALLKSHQIQVLVTDHHLP
                     PESLPNADALVNPNLAFCEFPSKSLAGVGVIFYVMMALRSRMREQHLFENKPEPNFAE
                     LLDLVALGTVADVVPLDQNNRILVHQGLQRIRSGHCRLGIKALAEVGKRDLSTLQAAD
                     LGFAIAPRLNAAGRLENMSLGVELLICENMELARRLALDLDSLNQTRKEFEQEMKAEA
                     LSICANLPSLAQSEQAHGIVLYQADWHQGVIGILASRIKDQFNRPVIAFAQESEESEY
                     LKGSARSINGVHMRDLLEHIDMRHPDLIEKFGGHAMAAGLTIHQSKLDLFKQIFDQSI
                     NAIIEPEQLQGIIYTDGELNALEFTLANAEMIRQGGPWGQHFPEPSFEGEFSILQQKL
                     LAGKHLKLMLQSEKGQLFDAIWFNVDVRAFPDLSIKKARLVYRLDINEFRGEKSLQLK
                     VEYLKYI"
     gene            4136..4984
                     /locus_tag="PXB27_000634"
     CDS             4136..4984
                     /locus_tag="PXB27_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDL5345456.1"
                     /translation="MEKKALFRGFLLSTVALAVAACGNLSKVSDEGTTENPVFPKISE
                     SEFNHDGSQFGSWPNWENVRQIEKGMNKDQLYNLIGRPHFEEGLYGVREWDYAFNYRE
                     NGVHKICQFKILFDKNMNAQSFFWYPNGCNGNSSFALTGDFLFDFDKATLTTKGKEVV
                     DNVAQQLKASKAQQVKVAGYTDRLGSAAYNLDLSQRRSNTVKARLVQQGVTAQIEAVG
                     YGKANQVKACDGETGQALKDCLRPNRRVEISANCGVMKQNEGGNVAGPNGPAPLYQTP
                     AYDGSK"
     gene            5072..5890
                     /locus_tag="PXB27_000635"
     CDS             5072..5890
                     /locus_tag="PXB27_000635"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
                     /protein_id="HDL5345457.1"
                     /translation="MNYQAIAFDLDGTLLSSNATILESSKEAIKKVREKGIKVYFVTG
                     RHHTAVRPYYVELGLDTPVVCCNGTYVYDFKNEKVLSGNPLSAEIASKLVNDAQAQGI
                     HTAVYFEDAMTYESLNIHFTKFQKWVESCAPEVRPNVYQVENFQQEIDKGVTVWKVLI
                     SDPDLAKMQKFVEKLPPDQVSPEWSWVDRVDITSVGNSKGARLAEVLKTEGIDLQKVI
                     AFGDNFNDVSMLETVGLGIAMGNSEPDIQQKAKKTIGSNNEDSIANELKAQFNL"
     gene            5893..7584
                     /gene="narQ"
                     /locus_tag="PXB27_000636"
     CDS             5893..7584
                     /gene="narQ"
                     /locus_tag="PXB27_000636"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HDL5345458.1"
                     /translation="MIKAKYSIARRIGLYFLIMIGFASIISGISLGIMWSNKSDASLI
                     NVSGSLRMQSYRLLSEMDSKKALLPERLLEYHRTLHATELSSLKQSVLLPDSVIESYQ
                     QLLKDWYEMQSYVAQGDKVNYASHIEDYVQRVDDFVFRLQEFAELKLKIATGVIAIAM
                     LLIIALAYIGVWYTRKKIIGPLRQLVKASWQIQNKDFDHVKLAVNEPNELGLLSSTFT
                     EMSNELLKLYTSLEDKVQDKTRRLLAVNRSLLVLYQCSQLLTAKPINKEVLNQVLKTV
                     FDNEHLLGIDLQVYGAEYWNVSIDNKPSLDWVSAEIAIENEKLGILSWKPSLVCPGER
                     LIQNVSEMIGRSLYVVQMQKQQQQLVLMEERAIIARELHDSLAQSLTFFKIQVSLLKL
                     AKTDEKRNEILTVFEKALNDAYSQLRELLTTFRLTIQEANLQQALEKILESLRPKTSA
                     QIRLSCKLPSHMFNAQQQVHVLQIVREAVINAIKHSQATEINVIAETKDDGEHCLTIQ
                     DNGVGLAAHTQPEGHYGLTIMQERAAELKAEFSIENVESGGVKVQVLLPSMISKR"
     gene            7635..8153
                     /locus_tag="PXB27_000637"
     CDS             7635..8153
                     /locus_tag="PXB27_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL5345459.1"
                     /translation="MQFRKAVEADFETILAIYNQAIPTHQITADLELATTQNRRKWFD
                     FHLETAQYPIWVVEDEKGIAGWFSFSPFYERPAFLHTSEISIYLDRYSKGKGYGSKII
                     EFMQAEMLNHNIHTLMAYVFELNTVSQNLMRKHGFEQWGRFPNIANMGKDEKGEDKWR
                     TLLMMSYQKGIE"
     gene            8196..8795
                     /gene="rdgB"
                     /locus_tag="PXB27_000638"
     CDS             8196..8795
                     /gene="rdgB"
                     /locus_tag="PXB27_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HDL5345460.1"
                     /translation="MEKTKIVLATSNADKVKEMADVLSQFGFEVVAQSEFGIESPEET
                     GLTFVENALIKARFAAKMTGLPAIADDSGLSVMALGGEPGLYSARYAGEQATDADNRQ
                     KLLAKMQGQENRLAKFVSCIVFLKHETDPTPYIASGECFGEILTEERGENGFGYDSLF
                     FYPPKACTFAELETKEKKAISHRAIALEQLKQQLAGENK"
     gene            8792..9112
                     /locus_tag="PXB27_000639"
     CDS             8792..9112
                     /locus_tag="PXB27_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HDL5345461.1"
                     /translation="MIITTTPTIEGKQIVEYKGLVFGEVVSGANFIRDFLAGITDIIG
                     GRSGAYESKLNGARKEALAELAKEAQKVGANAVVGVSMEYQSMGGDKGMFIVVATGTA
                     VVIR"
     gene            9116..9568
                     /locus_tag="PXB27_000640"
     CDS             9116..9568
                     /locus_tag="PXB27_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HDL5345462.1"
                     /translation="MQFEQHFEANFWQNKSLLEMNDAEWEALCDGCGKCCYRKYIQGR
                     GRRERLYYTSVACNLLDVNTGRCMNYSNRFKIESDCTKLTKKNLPDFGWLPKTCAYRL
                     LYEGKPLFDWHPLISKDPDSVKNAGMLIANGIHEKDVIDWFEFVIDEV"
     gene            complement(9609..10190)
                     /gene="lpcA"
                     /locus_tag="PXB27_000641"
     CDS             complement(9609..10190)
                     /gene="lpcA"
                     /locus_tag="PXB27_000641"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HDL5345463.1"
                     /translation="MYLEQIKAELQEAADVLDKFMSDEKNIKLIQDAAMLIANSFKQG
                     GKVISCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHLSCVSNDFGYEYVFS
                     RYLEAVGQKGDVLFGLSTSGNSKNVLNAIKVAKEKGMKVIAMTGKDGGQMAGLADVEI
                     RVPHFRYADRIQEIHIKVIHILMMLIEFEMEKA"
     gene            10499..11458
                     /locus_tag="PXB27_000642"
     CDS             10499..11458
                     /locus_tag="PXB27_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL5345464.1"
                     /translation="MVVNPESFEQIIGQSVKIKEVVEQAKKFANLDAPLLIQGETGTG
                     KDLFAKSCHHFGSRRMQKFIAVNCAGLPAEEAESEMFGHRGNGVESVGFFEYAHGGTV
                     LLDSISELSLEMQAKLLRFLNDGSFRRVGEDQEIQVNVRVICTSLQPLSLLVEKGKVR
                     EDLFHRLNVLTLNLPPLRDRKEDIPLLADYFIAQISNQLGVSKLEYDNVFLQALQNYH
                     WRGNLRELYNALYRACTLSHSYRLAVKDLHLPRDMEAMIEVREEATLEEMMSQYEAQL
                     LHQFYAEYPSTRKLAQRLGLSHTAVANKLRQYGLTKNGNSKDE"
     gene            11451..13202
                     /locus_tag="PXB27_000643"
     CDS             11451..13202
                     /locus_tag="PXB27_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5345465.1"
                     /translation="MNKLAVIFSVCFANLGLNSTAYAAPSIPQELLENSLVYCTTSSG
                     FSFNPQKSDVSSNMNVVTEQIYDKLVEFNPKKNRLEPALAESYSISDDGLVITLRLRK
                     NVAFHTTPWFTPSRKLNAEDVVFSLKRMIGKESDLPEVKSEPESVLHHQRQSYIYQKT
                     AGQIYFPYFESVDLANKIASIHSPSNDIVKIELVRPDSALLAHLASQYAVILSKEYAL
                     QLNADDNLVQLDLLPVGTGVYQLESYENNDYVRLKPNMNYWGKKAYIQNMVVDFSTEA
                     TGRMAKFLNNECDVVAFPEPSQLSVLNNRQLISSKGANLVYLAFNMQRGIGQDLALRQ
                     RIARGINRKRIAEKLFYGTGEVADNLLPTALWSKKNNGSYPYQPHQNSGEQAVEMHDS
                     IINRSLDSKTETEEVNQKQRLKLWVIDEKRVYNQHPLKMAEMIRADLATQNLEVEIRA
                     VSRAYLVQQLANKSADYDLILGGWLANNLDPNSFLEALLSCKAAETVTNLSNWCNEDF
                     DFWLQTARLSDDPASANLLYEFAQKLLEEQLPILPLMNANRVLVIKDGVKQADISPFG
                     QVKLSELRLGLGHKKGN"
     gene            13205..14170
                     /locus_tag="PXB27_000644"
     CDS             13205..14170
                     /locus_tag="PXB27_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL5345466.1"
                     /translation="MLIVFFRKIFLTLITLIFLSLISFNILLRDPLNHLAELGWFEAY
                     CYYVKSLLNGDFGISFSNGEPLTAQILQVFPATITLCLSALIVSLIIGLPLGFFAATQ
                     QKNMVGRLLNTLGSLSLAVPVFWLALVLMYYASLNQWEVSAIGEIHSLYPKQMVTGFL
                     LLDIWLSDVPYKLKMMQSALHHLALPTLVLAVPATLEVMHITQARAGYVMKQNYIKIA
                     KTRGWTPFRVWRTHIICNTLPALLPMIARTFILIFAFGMLIENIFSWGGIGRWLINAL
                     AIQDYNAISAGVVAIGVFVLLVDMVTGFVRVMLDPSDKKDWYGSY"
     gene            14157..15050
                     /locus_tag="PXB27_000645"
     CDS             14157..15050
                     /locus_tag="PXB27_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL5345467.1"
                     /translation="MAVIKEPEQFRETNAFKAFWFHFRQDKLALGSFYLFLLLLALIF
                     VGSLLAPYNYNEQFVGLELMPPSWDDKGQISHFLGTDDLGRDILSRIMYGFYYTLGSA
                     LMITLLIAIIGGMIGIYAGLKKGRSFWLISHLFDTFLFMPILLIAIIIATLMEASLVN
                     AMLAIFLAMLPHFIHKIYQATEKQLKKEYIITLKLDGASCWYLIREVIIPNLTGVAIK
                     ALSHIFVIAILDISALSFIMLGAKSPTPEWGAMIRESMELIYIAPWTVILPGLAIIFS
                     IFTVTILGTRLSVIFEKYRKE"
     gene            15121..16173
                     /locus_tag="PXB27_000646"
     CDS             15121..16173
                     /locus_tag="PXB27_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL5345468.1"
                     /translation="MALLDIRHLSIEIDMPKGRVKMVDNINLTVDEGEICGLVGESGS
                     GKSLIAKVICGMIKDEWIVSADRFRFDDIELLKLSPAERRKLVGDHISMVFQDPLTSL
                     DPSQQIGKQLMQTIQFRGKWWQRFGWKKKKAIELLHRVGIRDHQDIMKSYPSDITEGE
                     AQKVMIAMAVANQPRLLVADEPTNIMESTTQLQVYRLLSSMNQNLGTSILLVSNDMRS
                     IQNWVDSYNVLYCGQTVEIADKESILESSYHPYTRVLLNSSPDFSQPLAFKSSLNTLR
                     GSVPMLQEMPIGCRLGPRCPFAQKKCINKPPLVKVKQHEFACHFPINFREQNLLKNKT
                     ENTPFVVAEIAEKEAF"
     gene            complement(16182..16412)
                     /locus_tag="PXB27_000647"
     CDS             complement(16182..16412)
                     /locus_tag="PXB27_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HDL5345469.1"
                     /translation="MKLTACKLVFLLYLMFNLTACGTVLSLVEQDYRVYAGVIKDFQV
                     MQEGGIFAVLAVIDLPLSFVLDTLMLPVTLSQ"
     gene            16542..17624
                     /gene="leuB"
                     /locus_tag="PXB27_000648"
     CDS             16542..17624
                     /gene="leuB"
                     /locus_tag="PXB27_000648"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDL5345470.1"
                     /translation="MQTYNIAVLAGDGIGPEVMAEAIKVLEATQQKFGFKLNFNQYNI
                     GGAAIDAQGKALPENTLKGCEEADAILFGSVGGPKWTHLLPDEQPERGSLLPLRKHFS
                     LFCNLRPAALYQGLEKFCPLRADIAAKGFDMVVVRELTGGIYFGQPKGRDGEGAQTRA
                     FDTEVYYKYEIERITRVAFESAMKRKKHVTSVDKANVLQSSILWRETVTEVAKEFPEV
                     TLEHMYIDNATMQLIKAPESFDILLCSNIFGDIISDEAAMITGSMGMLPSASLNEQGF
                     GLYEPAGGSAPDIAGKNIANPIAQILSAAMMLRYSFNLGDAADAIETAIQKALADGYR
                     TADLADDSKPLSTSEMGDIIAKNILA"
     gene            complement(17711..18106)
                     /gene="msrB"
                     /locus_tag="PXB27_000649"
     CDS             complement(17711..18106)
                     /gene="msrB"
                     /locus_tag="PXB27_000649"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDL5345471.1"
                     /translation="MIKPIHALTEQQVDILINHGTERPFTGKFLNEHRVGIYRCARCH
                     NDLFHSDTKFDAGCGWPSFYQTISESSLRYLDDYSLGRHRIEIRCGNCDSHMGHVFPD
                     GPPPTGLRFCLNSAALNFKWEETGEEIDG"
     gene            18331..19335
                     /gene="gap"
                     /locus_tag="PXB27_000650"
     CDS             18331..19335
                     /gene="gap"
                     /locus_tag="PXB27_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010129843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDL5345472.1"
                     /translation="MAIKIGINGFGRIGRIVFRAAQHRDDIEVVGINDLIDVDYMAYM
                     LKYDSTHGRFDGTVEVKDGQLVVNGKAIRVTAERDPANLKWNEIGVDIAVEATGLFLD
                     DETARKHITAGAKKVVLTGPSKDATPMFVNGVNFDTYAGQDIVSNASCTTNCLAPLAK
                     VIHEKFGIKEGLMTTVHATTATQKTVDGPSAKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPALNGKLTGMAFRVPTTNVSVVDLTVNLEKPATYAEICAEIKRASENEMKGVLGYTE
                     DAVVSTDFNGATETSVFDAAAGIALTDTFVKLVSWYDNEVGYSNKVLDLVAHVYNYKG
                     "
     gene            complement(19670..19746)
                     /locus_tag="PXB27_000651"
     tRNA            complement(19670..19746)
                     /locus_tag="PXB27_000651"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19710..19712),aa:Val,seq:gac)
     gene            complement(19810..20610)
                     /gene="apaH"
                     /locus_tag="PXB27_000652"
     CDS             complement(19810..20610)
                     /gene="apaH"
                     /locus_tag="PXB27_000652"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HDL5345473.1"
                     /translation="MATYIVGDLHGCFNEFQLLLEKVNYNPKQDELFLTGDLVARGED
                     SLACLRFVKDSANNAKTVLGNHDLHLLGIKRVKPNDKIDAIFEAKDRLDLQNWLRNQP
                     LVIHHPKHQFLLTHAGISPEWDLATTLACAKEAEKVLQSDNYADYIAQMYENKPERWS
                     EDLQGVERWRYIINVFTRMRFCYTDKRLDFDCKLPLEQAPQELLAWFDLDNPLFTQKN
                     IIFGHWASLMGKCTRPNIYALDTGCAWGSHLTMLRWEDKQIFTQKRLK"
     gene            complement(20702..22678)
                     /locus_tag="PXB27_000653"
     CDS             complement(20702..22678)
                     /locus_tag="PXB27_000653"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HDL5345474.1"
                     /translation="MFQNNPLLAQLKQQIEASKEYVAGVVKASDKAFGFLECDKKSYF
                     IPPAEMKKVMHGDSVKAVVKRDGDKEQVEIDSLLEPMLDRFIAQVRLNKEGKLQLAVD
                     HPSIKQTIPANTHKKATEKLENGDWVVAQLKTHPLRDDRFFFAQVTQFICKESDNFAP
                     WWVTLARHEQPREPVANEKNYELHDELAREDLSHLYFTTIDSPSTQDMDDALFIEPIK
                     QNGKRTGWRLVVAIADPTAYIPENSHIEKAARQRCFTNYLPGFNIPMLPRELSDDLCS
                     LVPNEKRPALVGFIETDLNGNLIKETTFTSAWVESKDRLAYDDVSDYLEGVENAWQPQ
                     SEETKQQIEWLHQFTQARIQWRAENALLFKEQGDYTFELNEDGSVKDIHVEYRRIANQ
                     MIEESMILANICCAKFLSQHAKTGVFNTHSGFDPKNYELVKSFLLQTLASDENRDELA
                     ERYSAERLATLEGYCQMQRDIQDYPEKFLELRLRRYLTFAEFKAEVAPHFGLGITDYA
                     TWTSPIRKYGDMVNHRLIKQVLLGKQSQAVEESVLSRLQEARRQNRLVERDIADWLYA
                     RYLAPMVEQAVEFECEIADVSRGGLRAKVIKNGAQIFVPFSSLHDNKEEMEYRQEELA
                     LYIKGEKAYQIGQAVKVKLTEVRLETRSIVGNII"
     gene            22838..23116
                     /locus_tag="PXB27_000654"
     CDS             22838..23116
                     /locus_tag="PXB27_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bor family protein"
                     /protein_id="HDL5345475.1"
                     /translation="MKKLLAIATVFSLAACSTQTLELSPAKEAQYDRVQHFFVSGLAQ
                     KQEIDAAKICNGADKVGKVETETTFFNGLLGTITYGIYSPRQIRVYCN"
     gene            23141..23437
                     /locus_tag="PXB27_000655"
     CDS             23141..23437
                     /locus_tag="PXB27_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bor family protein"
                     /protein_id="HDL5345476.1"
                     /translation="MKKLLAMSVLTALLTTACTTQTAHIGGKVTDNIKPTKSVTQSFF
                     IGGIGQEETLNVVEVCGSKDKVQQVETVLSGGDILLGLVTIGIYTPRTANVYCK"
BASE COUNT         7339 a         4045 c         5002 g         7051 t
ORIGIN      
        1 agagatcgta agcgacataa aaaacatttt aagattgcgg ttttcttatt agcggtattt
       61 agtctatttg ttgatattgt tctcgcacta aaagatcaat cctcaatcaa taatacatct
      121 tcacaggaag atattattgc cattgataaa caggaaaaat atcagagtat caacactaat
      181 ttggcggaaa ataatactct ccatcaggag caacattcca gttttattaa ttatgtgact
      241 gatttactga ctcagtcggc attagtaatc gaacatgacg acgaaattgt tgatgatcaa
      301 acagcgactt cttatgaaga tgaattagtt gggcaagacg atgaagtaga agaagggatt
      361 gaaacggttg aaaatcaagt tcctggggaa aaactcccag aagaagctga aaaggcatta
      421 gaagatttac ttgatgtggc tgatcaagca ctacgaattc aaaaccaatt cagttatacc
      481 gtagctcgag gtgataaact taaagatgta ttagagcaat caggcttaac tgcatccgtt
      541 gcgaaaaatt taattaagca acattcggca ttagcacaat tagaaccggg tcaacaattt
      601 tattgggtat tagataatga cggtgaatta gaatatatga actggctggt ttccgaaaaa
      661 gaggaacggg tttatgagcg aaaagaaaat ggtaaatttt cttttcaaag atttgagaaa
      721 aaaggggaat ggaagttaga tgttgtaaga ggaactattc agaatagttt tgcggttagc
      781 ttaaaagccg ttggcttatc agaccgccaa attaaccagc tagcggttgg attgcagtca
      841 caaattgcga cgaataaatt aaaaaaaggg gataggtttg caattctgat taagcgggaa
      901 tatattgacg gcaaagtaac ggatttagga aatgtggaag gaatccatgt aattagcagt
      961 aaaaaaagtt attatgctat tcaagcagat aatggacgct attatagtcg atatggtgaa
     1021 acattaagtg gaggatttgc tcgtcatcca ttattatttg cagcaagagt aacttcttct
     1081 ttcaacccca aacgcagaca ccctattaca gggagaatta gtccgcataa aggggttgac
     1141 tatggcgtgc caataggtac tccgatcatc gcaccaagtg atggtgtcgt agagcatgtg
     1201 gcatatcaag cccgtggagc aggacgttat attcggataa aacacggaca tattacgact
     1261 gtttatatgc acctcagcaa aacgttggta aaaccagggc agagtgttaa aaaaggggag
     1321 cggatagcat tatcgggtaa tacaggcggt tcaaccgggc ctcatttaca ctatgaattc
     1381 catattaatg gtcgaccggt caacccgatt acggtaaaat tgcctggctc aaattcaggt
     1441 atgggtaata aagaacgtca agcattctta aataaagcga agaatgtaga aagtagatta
     1501 aaaatgtaag gattctcaat gaaaaaaaat ctaatcactt taggattaat gtgtttatca
     1561 accatcacgt ttgcttcaga tgcaaaacta aaaagccagc ttgaaaaaat tggagccaca
     1621 gatgttaaaa tttcagattc tccattaaca ggctttaaaa tggcgacttc aaatgaaggt
     1681 gttgtatata tcagccaaga cggtcgttat gtaattcaag gtaaagtatt tgagctaaaa
     1741 aatggtaaag cgattgatat tactaatcaa ggattgttgg cagaattgaa ttcatacact
     1801 aatgaaatga tcgtatatcc agcgaaaaat gagaaacatg tgattacggt gtttatggat
     1861 attacttgcc attattgcca tttactacat actaaaatca aagaatataa tgatttaggt
     1921 attaccattc gctatttggc attcccgcgt ggtggaatga atacccaaac agcaaaacaa
     1981 atggaagcta tttggacatc agcagacaaa gtaaaagcac tgaatgatgc tgaagatggt
     2041 aaattaccaa cccaaataaa aacgccggat attgtgaaaa aacattacca attagggatt
     2101 aaatttggtg tgacaggtac cccaaatatg gtaacttcag aaggtgagtt gattggtggt
     2161 tatgtagagc ctaaagaatt agcgaaaatg ttaagtgagt aattcattaa cttgaataca
     2221 agcggtcttt ttttgtaaaa tttttgcaaa agaagaccgc ttgttgtttc attaaagaag
     2281 agtattgtgg aaaaaataat taaaaggcgc ccattattaa caacggaaat agtctctaca
     2341 aacccgttat taaatcgaat ctatcaaagt cgaggcataa agaatacgca agaactagag
     2401 cataatttaa agaatttgca tagaccacag caacttgcca atattgagca agccgttgac
     2461 ttgctattac aagcctttcg tgaaaaaagc cggatcatta ttgtgggtga ttttgatgca
     2521 gatggtgcaa ccagtacatc gcttgccctt attgcgttac gccaattggg tttcgataag
     2581 gtagattatt taattccgga tcgtttttct caggggtacg gattgagtgt agcggttgct
     2641 gaaatggtat tagcaaaagg ggcggattta gtcttaacgg tagataatgg aatttcttcg
     2701 actgaaggca ttgctctgct aaaaagccat cagattcaag ttttggttac cgatcatcat
     2761 ttaccgccag agagcctacc caatgcagat gcactggtaa accccaattt agctttttgt
     2821 gaatttccgt ctaaatcttt agccggggta ggcgtgatct tctatgtaat gatggcattg
     2881 cgttctcgta tgagagaaca acatttattt gaaaataaac cagagccaaa ttttgctgaa
     2941 ttactggatt tagtcgcttt aggcacagtg gctgatgtag tgccgttaga tcaaaataat
     3001 cggatattgg tgcatcaagg gttacagcga atccgttcag ggcattgccg tttagggata
     3061 aaagctttag cggaggtggg caagcgagat ctttctactt tgcaagcagc agatttgggt
     3121 tttgctatcg caccacgttt gaatgcagct ggtcggttag aaaatatgtc tttaggggtt
     3181 gagttactaa tttgtgaaaa tatggaatta gctcgccggc ttgcgcttga tcttgattct
     3241 cttaatcaaa cccgaaaaga gtttgaacaa gaaatgaaag cagaagcact gtcgatttgt
     3301 gcgaatttgc caagtcttgc gcagtctgaa caggcacacg gaatagtgtt atatcaagca
     3361 gattggcacc aaggtgtaat tgggatctta gcttctcgca ttaaagatca atttaaccgt
     3421 cctgtcattg cttttgcaca agaaagtgag gaaagtgaat atctaaaagg ttctgctcgc
     3481 tcgatcaacg gagtacatat gcgagattta ctggaacata tcgatatgcg acatccagac
     3541 ttgattgaaa aatttggtgg acacgcaatg gcggctggtt tgactatcca tcaatcaaaa
     3601 ttagatttat ttaaacagat atttgaccaa tctattaatg caattatcga gcctgagcaa
     3661 ttacaaggca tcatttatac cgatggtgag ttaaatgcac tagagtttac tttagccaat
     3721 gccgagatga ttaggcaagg tgggccttgg ggacaacatt ttccagaacc tagttttgaa
     3781 ggggagttta gcattctcca acaaaaatta ctcgcaggta aacatttaaa attaatgttg
     3841 caatcagaga aagggcaact atttgatgca atttggttta atgtggatgt aagggcgttt
     3901 cctgacttat cgatcaaaaa ggcaagatta gtttataggt tggatattaa tgaatttcga
     3961 ggagagaaat ctttgcaatt aaaagtggag tatcttaaat atatttgaaa tgttatttta
     4021 tgtaaattta atagaattaa ttatatttgt attgtagaat ccgtccgaat gcaactttaa
     4081 taggttgtga agctattttt ttatacagat tactataagg aaacttaata tgaaaatgga
     4141 aaaaaaagcc cttttccgtg gatttttatt atcgacagtg gcattagcag tggctgcttg
     4201 tggtaactta agtaaagtga gtgatgaagg aacaactgaa aacccggtat tcccgaaaat
     4261 ctcagaatct gaatttaacc acgatggttc acaatttggt tcatggccaa actgggaaaa
     4321 cgttcgtcaa attgaaaaag ggatgaacaa agatcaatta tataatctaa ttggtcgtcc
     4381 tcattttgaa gaaggcttat atggtgttcg tgaatgggac tatgcattca attaccgtga
     4441 aaatggcgta cataaaattt gccaatttaa aattttattc gataaaaata tgaatgcaca
     4501 aagtttcttc tggtatccaa atggatgtaa tggaaactca tcatttgctt taactggcga
     4561 tttcttattc gatttcgata aagcgacttt aactacaaaa ggtaaagaag ttgttgataa
     4621 tgttgctcaa caattaaaag catctaaagc acaacaagtt aaagtagcag gctatacaga
     4681 ccgtttaggc tctgctgctt ataacttaga cttatcacag cgtcgttcta atacagttaa
     4741 agctcgttta gtacaacaag gcgttacagc acaaattgaa gcggtaggtt atggtaaagc
     4801 gaatcaagta aaagcttgtg atggtgaaac tggtcaagca ttaaaagatt gtttacgccc
     4861 taaccgtcgt gttgaaattt ctgcaaattg tggtgtaatg aaacaaaatg aaggtggcaa
     4921 tgttgcaggt cctaatggtc cggctccact ttatcaaacc ccagcatatg atggcagtaa
     4981 ataatttcta gttttgacta acttagagta gaatattaag gcaaccaaat ggttgccttt
     5041 tcttttatct atttatttat aggggaatac tatgaactac caagctattg catttgattt
     5101 agacggaaca ctactttcat cgaacgcaac aattttagaa tccagtaaag aggctattaa
     5161 aaaggtaaga gaaaagggaa ttaaagtcta ctttgttacc ggtaggcacc atactgcggt
     5221 tcgcccttat tatgtagaac tagggctgga tacgccagtt gtgtgttgca atggcactta
     5281 tgtgtatgat tttaaaaatg aaaaagtatt aagcggtaat ccgttatcag cagaaattgc
     5341 ttcaaaatta gttaatgatg cacaagctca agggattcat actgcggttt attttgaaga
     5401 tgcaatgact tatgagtctt tgaatattca cttcactaaa ttccaaaaat gggttgagtc
     5461 ttgcgcacca gaagttcgtc cgaatgttta tcaggtagaa aatttccaac aagaaattga
     5521 taaaggtgta accgtatgga aagtgcttat tagcgatcca gatcttgcca aaatgcaaaa
     5581 atttgttgaa aaattaccgc ctgatcaagt aagcccggaa tggtcttggg ttgatagggt
     5641 tgatattacc agtgttggta acagcaaagg ggcaagatta gcggaagtat taaaaacaga
     5701 aggtattgat ttacaaaagg tgattgcctt tggcgataat ttcaatgatg tttctatgct
     5761 agaaaccgtt ggattaggca ttgcaatggg aaattcagaa ccagatattc agcaaaaagc
     5821 gaagaaaact ataggttcaa ataatgaaga tagtattgct aatgaattga aagctcaatt
     5881 taatctttaa ttatgataaa agcaaaatac tctattgcta ggcggatagg actctatttt
     5941 ttaattatga tcggatttgc ttcgattatc agtggaatct cacttggtat tatgtggagc
     6001 aataaatctg atgcgagcct gattaatgtt tcaggctcac ttcgtatgca aagttatcga
     6061 ttgttatcag aaatggattc aaaaaaagcg ctgctacctg agcgattgtt ggaatatcat
     6121 cgaacgctac atgctacaga gcttagttca ttaaagcaaa gtgttttatt gccagatagc
     6181 gtgatagaaa gctatcaaca attacttaaa gattggtatg aaatgcaatc ttatgtcgct
     6241 cagggagata aagttaatta tgcttcgcac attgaagatt atgtgcagag agttgatgat
     6301 tttgttttcc gcttacaaga gttcgctgag ttaaaattaa agattgcaac aggcgtgatt
     6361 gcgattgcaa tgttactgat tattgccctt gcttatattg gcgtttggta tactcgaaaa
     6421 aagattattg gacctttaag gcaattggta aaagcgagtt ggcaaattca aaataaagat
     6481 tttgaccacg taaaattagc ggttaatgaa cccaatgaat tagggctact ttcctctact
     6541 tttactgaga tgtctaatga attattaaaa ttatatactt cattagaaga taaagttcag
     6601 gataaaacac gtcgtttatt ggcggtaaat cgttctttac tggtattata tcaatgctct
     6661 caattattaa ctgctaagcc gattaataaa gaagtgttaa atcaagtact taaaacggta
     6721 tttgataatg aacatttgct agggattgat ttacaagtat atggggctga atattggaat
     6781 gtatcaatag acaataaacc aagtttagat tgggtttctg ctgaaattgc aatagaaaat
     6841 gaaaaattgg gtatcttaag ctggaaaccc tcactggttt gtcctggtga acgattgatc
     6901 caaaatgtgt cggaaatgat tggtcgtagc ctttatgtag tgcagatgca aaagcaacag
     6961 caacaattgg tactcatgga ggagcgtgca attattgctc gtgagttaca cgattcctta
     7021 gcccaatctc ttacgttttt caaaattcag gttagcctgc ttaagcttgc taaaacagat
     7081 gaaaagcgta atgaaatttt aactgtattt gaaaaagcgt taaatgatgc ttacagccaa
     7141 ttaagggagc tgttgacgac gtttaggcta acgattcaag aagcgaattt acaacaagcg
     7201 ttagaaaaaa ttttagaaag cttaagaccg aaaacctcag cacagattcg cttaagttgc
     7261 aaattacctt cgcatatgtt taatgctcag cagcaagttc acgtattgca aattgtacga
     7321 gaagctgtga ttaatgctat taagcattcg caagccactg aaattaatgt gattgctgaa
     7381 acaaaggatg atggtgaaca ttgtttgact attcaagata atggcgtagg gcttgctgcc
     7441 catacgcaac cagaagggca ttatggactg acgattatgc aagaacgggc ggctgaactc
     7501 aaagccgaat tttcgattga aaatgtggaa agtggcggag taaaagtaca ggttttattg
     7561 ccgagtatga ttagcaagcg gtaataaaat attaattttt tgcgtattat tggcaacgaa
     7621 taaaaggata acaaatgcaa ttccgaaaag cagtagaagc ggattttgag acaatattgg
     7681 caatttataa tcaagcaatt cccactcacc aaattaccgc cgatttagaa ctggcaacta
     7741 cgcaaaatcg gcggaagtgg ttcgattttc accttgaaac agcccaatat cctatttggg
     7801 tggtcgaaga tgaaaaaggc attgctggct ggtttagttt ttcaccattt tatgaaagac
     7861 cggcattttt acatacttcg gaaatcagta tctatttaga ccgctattcc aaaggaaaag
     7921 gctatggctc aaaaattatt gagtttatgc aagctgaaat gcttaatcac aatattcata
     7981 cgttgatggc gtatgtgttt gaattaaata cagtgagcca aaatctaatg agaaaacacg
     8041 gctttgaaca atggggaaga tttccgaata ttgctaatat gggaaaagat gagaaaggtg
     8101 aggataaatg gcgaacgttg ctgatgatgt cttaccaaaa aggtatcgaa taacaagcgg
     8161 tgatttttta gaatattttt gcaaataagg acaatatgga aaagacaaaa attgtacttg
     8221 caacaagtaa tgcagataaa gttaaagaaa tggcagatgt actctcacaa tttggttttg
     8281 aggtggtggc tcaaagtgaa tttgggattg aatcaccgga agaaaccggt ttgacttttg
     8341 ttgaaaatgc gctgattaaa gcacgatttg ctgctaaaat gacaggttta ccggcgattg
     8401 ccgatgattc aggtttatcg gttatggcac ttggtggtga accggggcta tattctgcac
     8461 gttatgccgg agagcaggca accgatgccg ataatcgcca aaaattacta gccaaaatgc
     8521 aagggcaaga gaatcgtctg gcgaaatttg tcagttgtat cgtgttttta aaacacgaaa
     8581 ctgacccaac accttatatt gcgtcgggtg aatgtttcgg tgaaattttg acggaagaaa
     8641 gaggtgaaaa tggtttcggc tacgattccc tgtttttcta cccaccgaaa gcttgcactt
     8701 ttgccgaatt agaaaccaaa gagaaaaaag cgatttcaca ccgtgcaatc gcactagagc
     8761 aattaaaaca gcaattagca ggagagaata aatgattatt acaactacac ctacgataga
     8821 gggtaaacaa attgtagaat acaaagggtt agtatttggc gaagtagttt ctggggctaa
     8881 ttttatccgt gatttcttag ccggtatcac cgatattatt ggcggacgtt caggtgctta
     8941 tgaaagcaaa ttaaacggtg caagaaaaga ggcattagct gaattagcta aggaggcaca
     9001 aaaagtgggg gcaaatgcgg tggttggcgt ttctatggag tatcaatcaa tgggcggcga
     9061 taaaggaatg ttcatcgtgg tagcgactgg tactgcggtt gtaatacgtt aatttatgca
     9121 gtttgaacaa cactttgaag caaatttctg gcagaacaaa tcgctacttg aaatgaatga
     9181 tgctgaatgg gaagcattat gtgacggttg tggtaagtgc tgctaccgga aatatattca
     9241 gggaagaggg cggcgtgaac ggctttacta tacctcggtt gcctgtaatt tattagatgt
     9301 gaatacaggc cgttgtatga attactcgaa ccgttttaaa attgagtcgg attgtacaaa
     9361 attaactaaa aagaacttac ctgattttgg ctggttgcca aaaacctgtg cttatcgttt
     9421 actttatgaa ggaaagcctc tttttgattg gcacccttta atctctaaag atcctgattc
     9481 tgtaaaaaat gccggaatgc tgattgctaa cggtattcac gaaaaagatg tgattgattg
     9541 gtttgagttt gttattgatg aagtatgaga aaaaggcgtt tggtgcaaac gcctttttgt
     9601 tatagagatt aggctttttc catctcaaat tcaattaaca tcatcaaaat atgaatcact
     9661 ttaatgtgaa tttcttggat gcggtctgcg taacggaaat gaggtactcg gatttccaca
     9721 tcggctaatc ctgccatttg cccgccatct ttgccggtca ttgcaatcac tttcatgcct
     9781 ttttctttgg caactttgat cgcatttaac acatttttag agttaccaga ggttgataga
     9841 ccaaagagta catcgccttt ttgcccaacg gcctctagat aacgagagaa gacatattca
     9901 taaccgaagt cattacttac acagcttaaa tggctgacat cggaaatagc gattgccggg
     9961 tatccgggac ggttttcacg gtaacgacct gttaattctt cggcaaagtg catcgcatca
    10021 cagtgcgaac cgccattacc gcaagaaatc accttaccac cttgcttgaa actatttgca
    10081 atcagcatgg cggcatcttg aattaattta atattttttt catcagacat aaatttgtct
    10141 aatacatctg cagcttcttg aagctcagct ttaatttgtt ctaaatacat catttgctcc
    10201 tttgtttgca aaacgcttac ctttaaacat cggctttacc aatgggggta gcgagtaaaa
    10261 aattcgtttt ttatgaccgc ttgaggcata aaaagtgcaa taagtttaga agatctatgc
    10321 tgtattgtaa acctaataac ttaaataatc tgaatagggt tgtaaacaaa agtttccaaa
    10381 agagaaaaat agcggtatga tcgcctcaca atccctctcg cattgttaac tctagtagct
    10441 aatatcaggc tttaagcgta ttaaacaatg cacaaatttg ctatttatag gagactatat
    10501 ggtcgtaaat ccagaatctt ttgaacagat aattggacaa tctgtaaaga taaaagaggt
    10561 tgttgaacaa gcaaaaaaat ttgccaacct tgatgcacca ttattgattc aaggtgagac
    10621 gggaacagga aaagatttat ttgccaaatc ttgtcaccat tttggatctc gtcgaatgca
    10681 aaaatttatt gccgtgaact gtgccggttt accggctgaa gaagcagaat cggaaatgtt
    10741 cggacatcgt ggcaatggcg ttgagtccgt cggtttcttt gaatatgctc acggtggtac
    10801 agtattatta gactctattt ctgagttatc gctggaaatg caagccaaat tattgcgctt
    10861 tttaaatgat ggctcttttc gtcgagtggg cgaagatcaa gagattcaag tcaatgttag
    10921 ggtgatctgt acttcattac agcctttgtc attattagtt gaaaaaggca aagtaagaga
    10981 agatctgttt catcgtttaa atgttctcac tttaaattta ccacctttgc gagatcgaaa
    11041 agaggatatt ccattattag ctgattactt tatcgcccaa attagcaatc agttgggggt
    11101 gagcaaatta gagtatgata atgttttttt gcaagcattg cagaattatc actggcgagg
    11161 caacttaaga gagctttata acgcacttta tcgagcttgt acactttcgc atagctaccg
    11221 tttagcggta aaagatcttc atttacccag agatatggag gcgatgattg aagtacgtga
    11281 ggaggcgaca cttgaggaaa tgatgagcca gtatgaagcc caattattgc accaatttta
    11341 tgcagaatat ccgagtaccc gaaaattagc acaacggctc ggactttcac atactgcggt
    11401 ggcaaacaaa ttgcgtcaat atgggttaac aaaaaatgga aacagtaaag atgaataaac
    11461 tagcggtcat tttttcagta tgttttgcaa atttagggct aaattcgacc gcttacgcag
    11521 cgccaagtat tcctcaagag ctacttgaaa atagccttgt gtattgcact acttcatcgg
    11581 gattctcttt taacccacaa aaatccgatg tgagttcgaa tatgaatgtg gtaacagagc
    11641 agatctacga taaattagtt gagtttaatc cgaaaaaaaa tcgactagag cctgctttag
    11701 ctgaaagtta ttcaatcagc gacgatggtt tagtgattac attacgttta agaaaaaatg
    11761 tcgcttttca tacaactcca tggtttactc cgagtcgtaa attaaacgca gaagatgtgg
    11821 tgttttcgct aaaacgaatg attggtaaag aaagtgattt acctgaagtg aaaagtgagc
    11881 ctgaaagtgt attacaccac cagcgacaaa gctatattta ccaaaaaaca gcggggcaga
    11941 tttattttcc ttattttgaa agtgttgatt tagcgaataa aattgccagc attcattctc
    12001 cgtcaaatga cattgtaaaa attgaattag tccgtcctga ttcagcattg ttggcgcatt
    12061 tagccagcca atatgcagtt attctgtcta aagaatatgc tttacagctc aatgcagatg
    12121 ataacttagt tcaactggat ttattaccgg taggaacagg cgtatatcaa cttgaaagtt
    12181 atgaaaataa tgattatgtg cgtttaaaac caaatatgaa ttactggggc aaaaaagcat
    12241 atattcaaaa tatggtcgta gatttttcca cagaagcgac cggcaggatg gcaaaatttt
    12301 taaataatga atgtgatgtg gttgcttttc cggaacctag ccaattatca gtgttaaata
    12361 atcggcagct tattagtagt aaaggtgcta atttggttta tttagcattc aatatgcaac
    12421 ggggtattgg gcaggatttg gcattacgcc aacgtattgc acgcggcata aatcgcaaac
    12481 gtattgcaga gaaattattt tatggtacag gtgaagtagc ggataaccta ttaccaacag
    12541 ctttgtggtc gaaaaaaaat aatggaagct acccttatca accacaccaa aattcaggtg
    12601 agcaagcggt tgaaatgcat gacagtatta taaatcggtc tttagatagc aaaacagaga
    12661 ccgaagaagt aaaccaaaaa cagcgcttaa aactgtgggt aattgatgaa aaacgagttt
    12721 ataatcaaca cccattaaaa atggcagaaa tgatacgtgc tgatttagct acgcaaaatt
    12781 tagaggtcga aattcgcgca gtaagtcgag cttatttggt gcaacagctt gctaataaaa
    12841 gtgcggatta tgatttgatt ttaggtggtt ggttagcaaa taatcttgat cctaacagtt
    12901 ttttggaggc cttactcagt tgtaaagctg cggaaacggt gactaattta tcaaattggt
    12961 gcaatgaaga ttttgatttt tggttacaaa cagcccgtct aagtgatgat ccggcatcag
    13021 ctaatttatt gtatgaattt gcacaaaaat tattagaaga gcaattgcct attctccctt
    13081 taatgaatgc taatcgtgta ttagtcataa aagatggagt gaaacaggca gatatttccc
    13141 ctttcggcca ggtaaaatta tcagagttac gtttgggctt ggggcataaa aaaggaaact
    13201 aattatgctg attgtctttt ttcgaaaaat ttttctaacc ttgattacgt tgattttttt
    13261 gtcacttatc agttttaata ttctgttacg agatccatta aaccacttag ccgagttagg
    13321 ctggtttgag gcttattgtt attatgtaaa atctctgcta aatggtgatt ttggtattag
    13381 tttcagtaat ggagaaccgc tcacagcgca aattttacag gtgttcccgg ctacaattac
    13441 gctttgttta tcggctctca ttgtttcttt aattattggt ttgcctcttg gtttttttgc
    13501 ggctactcag caaaaaaata tggttgggcg attattaaat acactaggtt cattgagttt
    13561 agccgtgccg gtattttggc tggcattagt gctaatgtat tacgcttctc tcaatcagtg
    13621 ggaagtgtca gcaattggag aaatccattc gctttatccg aaacagatgg taaccggctt
    13681 tttgctatta gatatttggt tatctgatgt accttataaa ttaaaaatga tgcagagtgc
    13741 tttacatcac ttagctttgc cgacattagt cttggctgtg ccagctacat tagaagttat
    13801 gcatattact caagctagag caggttatgt aatgaaacaa aattacatta aaatagcgaa
    13861 aacgcgtggc tggacaccgt ttagagtttg gagaacgcat attatttgta atactttacc
    13921 tgcattatta cctatgattg cccgtacctt tattttaatt tttgccttcg gtatgttaat
    13981 tgagaacata tttagctggg gaggaattgg acgttggctg ataaatgccc ttgctattca
    14041 agactataat gcgatttcag cgggagtagt tgcaatcggg gtgtttgtgt tattagtaga
    14101 tatggttaca ggctttgtgc gggtgatgtt agacccatca gataaaaagg attggtatgg
    14161 cagttattaa agaacccgag caatttagag aaaccaacgc atttaaggca ttttggtttc
    14221 atttccgaca agataaactt gcacttggca gtttttatct ttttttgcta ttactggctc
    14281 tcatttttgt tggtagtttg cttgctcctt acaattataa tgaacaattt gttggtttag
    14341 aattaatgcc tccctcttgg gacgacaaag gtcaaatcag ccatttttta ggcactgacg
    14401 atttaggacg ggatatttta agccgtatta tgtatggttt ttactacacg cttggttccg
    14461 cactgatgat taccttatta attgcgatta tcggcggtat gatcgggatt tatgccgggc
    14521 tgaaaaaagg acgttcattt tggttgatta gccatttgtt cgataccttt ttatttatgc
    14581 cgattttatt gattgcgatc atcattgcaa ccttaatgga agcgagtttg gtcaatgcaa
    14641 tgttggcgat tttcttagcg atgttgccgc attttatcca taaaatttat caagcaaccg
    14701 aaaaacaact caaaaaagag tacatcatta ccttaaagct agacggtgca agctgctggt
    14761 atttaattcg agaagtgatt attcctaatc ttaccggcgt ggcaattaaa gcactttcac
    14821 atatttttgt gatcgccata ttagatatta gtgcgttaag ttttattatg ctgggggcta
    14881 aaagcccgac ccctgaatgg ggagcgatga ttcgtgaatc catggaactg atttatatag
    14941 ctccttggac ggtgatatta cctggcttag caattatttt tagtattttt accgttacaa
    15001 tacttggtac acgcttaagc gtgatttttg aaaaataccg taaagaatag tatttaaata
    15061 ggatactgac acataacaag cggtcatttt taagtaaaat tttacagatt aaggaatagt
    15121 atggcattac ttgatattcg ccatttaagt attgaaattg atatgccaaa aggcagagta
    15181 aaaatggtgg ataacatcaa cctgactgtt gatgagggcg aaatttgtgg cttggtggga
    15241 gaatctggtt cgggaaaaag cctcatagcc aaagtgattt gcggtatgat taaagatgaa
    15301 tggattgtga gtgcagatcg ctttcgcttt gacgatattg aattactgaa actttcgcca
    15361 gcggaacgcc gaaaattggt aggcgatcat atttcaatgg tattccaaga tccgcttact
    15421 agccttgacc caagccagca aattggtaaa caactgatgc aaaccattca atttagaggg
    15481 aagtggtggc aacgatttgg gtggaaaaag aaaaaagcaa tcgagctact acaccgtgtt
    15541 ggtattcgtg atcatcaaga tattatgaaa agttacccaa gcgatattac cgaaggcgaa
    15601 gctcagaaag tgatgattgc aatggcggtg gcaaatcagc cccgattatt ggtggcagat
    15661 gagccgacta atattatgga atcaaccacg cagttgcagg tttatcgctt gctttccagt
    15721 atgaatcaga atttgggaac ctcaatcttg ctggtgagta atgacatgcg tagcattcaa
    15781 aattgggttg attcttacaa tgtgctttat tgtggacaaa ccgttgaaat tgcggataaa
    15841 gagagcattc ttgaatcgtc ttaccacccc tatactcgag tgttactcaa tagttcgccg
    15901 gacttttctc agcctttagc ctttaaaagt agcttaaata cgctacgagg ctcagtcccg
    15961 atgttacaag aaatgccgat tggttgtcgt ttaggccctc gttgcccttt tgcacaaaaa
    16021 aaatgtatca acaaaccgcc attagtgaaa gtaaaacagc acgagtttgc ctgccatttt
    16081 ccgattaact ttagagaaca aaatttgctt aaaaataaaa cggaaaatac gccgtttgtg
    16141 gtggctgaaa tagcggaaaa agaggcgttt taatgtggtt attattggct aagtgtaacg
    16201 ggtaacatta aagtatctaa tacgaaactc aagggtaaat cgatcaccgc aagcaccgcg
    16261 aatataccgc cttcttgcat tacttggaaa tcttttatta cgccggcata aacacggtaa
    16321 tcctgttcaa ccaagcttag tactgtgcca caagcggtga ggttaaacat caaatagagt
    16381 agaaaaacga gtttacaagc ggtcaatttc atcaaaaaat ctccaaatac gcttttattt
    16441 tacgctattt ttatcgggtt ggcgtaaaat tcgttttaaa aacagctatt ttcaggcaga
    16501 atgtagccga atcgacatta aatatactaa gaggatctta tatgcaaact tacaacattg
    16561 cagtgcttgc cggtgacggc attgggccgg aagtgatggc agaagctatc aaagtgcttg
    16621 aagcgactca acaaaaattt ggctttaaac taaattttaa tcaatataac attggtggag
    16681 cagcgattga tgctcaaggt aaagcgttac ccgaaaatac cttaaaaggc tgtgaagagg
    16741 cagatgcgat tttgtttggc tcggtaggtg gcccgaaatg gacgcattta ctacctgatg
    16801 agcaacctga acgaggttcg ctattaccac tgcgtaaaca tttctcgcta ttctgtaatt
    16861 tacgccctgc tgcattgtat caagggctgg aaaaattctg cccattacgt gccgatattg
    16921 ctgctaaagg ttttgatatg gtggtggtgc gtgaattaac cggcgggatt tattttggac
    16981 agccgaaagg ccgtgatggt gaaggtgcgc aaacgagagc gtttgacacc gaagtatact
    17041 acaaatatga aatcgagcgt attacccgtg tggcgtttga atctgcaatg aaacgtaaaa
    17101 aacacgtaac ctctgtggat aaagccaatg tgttacaaag ctcgatttta tggcgtgaaa
    17161 ccgtcacgga agtagctaaa gagttcccag aagtcacgct tgagcatatg tatattgata
    17221 atgcgacaat gcagctaatc aaagcacctg aatcgttcga tattttatta tgctcaaaca
    17281 tcttcggcga tattatttcc gatgaggctg caatgattac aggttctatg ggcatgttgc
    17341 cttcagccag cctcaatgag caaggtttcg gcttatatga accggcaggc ggttcagcac
    17401 cggatattgc cggtaaaaat attgcgaatc cgattgcgca aattttatca gccgcaatga
    17461 tgttgcgtta tagctttaac ttaggtgatg ctgctgatgc gattgaaaca gcgatccaaa
    17521 aagcccttgc tgatggctac cgtaccgctg atttagctga tgacagcaaa ccgctttcta
    17581 cctcagaaat gggcgatatt attgcgaaaa atattctcgc ttaaggctag aagttaaata
    17641 gcacaagcgg tcattattcg ccaaattttt gcaaattttc cgctaataat gaccgcttgt
    17701 ttattactcg ttagccgtca atctcttcgc ctgtttcttc ccacttaaaa ttcaatgccg
    17761 cagagttgag gcaaaaacgc aaccctgttg gtggtgggcc gtccggaaaa acgtgtccca
    17821 tatgcgaatc acaattccca caacgaattt cgatacgatg acgtcctaaa ctgtaatcat
    17881 ctaagtaacg tagtgacgat tctgaaatcg tttggtaaaa acttggccag ccacaaccgg
    17941 catcaaattt agtatctgag tgaaataaat cgttatggca tctggcacaa cgataaattc
    18001 ctacccgatg ttcattcaaa aattttccgg taaacggacg ttccgtaccg tggttgatta
    18061 aaatatcaac ttgttgttct gtcagggcgt gaataggttt aatcatcagc aattctccta
    18121 agatagtagc gaagtcattg tagctttttt tgacctgaaa atcgttataa atttcaaaat
    18181 ttgacataga tcacaaaaaa tttatctgtt atattggaag ggttcttttg gcttgtcagg
    18241 aaagtgaaaa gatgtgctat aattcaagca tctttagtgt agtacataaa gaacaaattt
    18301 tgtttaactt taatataggt agaaaaatct atggcaatta aaattggtat taacggcttt
    18361 ggccgtatcg gtcgtatcgt tttccgtgca gcacaacacc gtgatgacat cgaagtggta
    18421 ggtatcaacg acttaatcga tgttgattat atggcttata tgctgaaata tgattcaact
    18481 cacggtcgtt ttgacggtac tgttgaagtt aaagacggtc aattagtagt taacggtaaa
    18541 gcgatccgcg taacagctga gcgtgacccg gctaacttaa aatggaatga gatcggtgtt
    18601 gatatcgcag ttgaagcaac aggtttattc ttagatgatg aaactgctcg caaacacatc
    18661 accgcaggtg cgaagaaagt tgttttaacc ggtccgtcta aagatgcaac acctatgttc
    18721 gtaaacggcg taaactttga tacttacgca ggtcaagaca tcgtgtctaa cgcttcttgt
    18781 acaactaact gtttagcacc attagcgaaa gttattcacg aaaaattcgg tatcaaagaa
    18841 ggtttaatga caactgttca cgcaactact gcaacgcaaa aaacagtaga tggtccatca
    18901 gcgaaagact ggcgtggtgg tcgtggcgcg tcacaaaaca tcattccttc atcaacaggt
    18961 gcagcgaaag cagtaggtaa ggtattacct gcattaaacg gtaaattaac cggtatggct
    19021 ttccgtgttc caaccactaa cgtttctgtt gttgatttaa ctgtaaactt agaaaaacca
    19081 gcaacttatg cagaaatctg tgctgaaatc aaacgtgctt cagaaaacga aatgaaaggc
    19141 gtattaggct acacagaaga tgcagttgtt tcaacagact tcaatggtgc aactgaaact
    19201 tcagtatttg atgcagcagc aggtatcgca ttaactgata ctttcgttaa attagtatct
    19261 tggtacgata acgaagttgg ctactcaaac aaagtattag acttagttgc tcacgtatat
    19321 aactacaaag gttaattgct aagttatatt ttaaatatag agccaaattc tgaacggaat
    19381 ttggctcttt tgctgcgaac aataaattga atatagtcgc gttttcccat aaaatcaatt
    19441 tcctacaaac aggaactaag cacagtaagg cagattttct tgctctttgc agtttatttt
    19501 gtgtaaataa atattattag agactacaaa cgattaataa atatcaagtt tcctatgaaa
    19561 aatgatgttt ttattaatgc tatattgctt tttgagagat gatttaaatt attgattata
    19621 agtatggtaa gattaaagcc tgaatcggga tattcaggct taaaaataat ggtgcgacta
    19681 gctggactcg aaccagtgac ccccaccatg tcaaggtggt gctctaacca actgagctat
    19741 agtcgcatta acggtgtgca ttctattgga aaaaggaact tagtgcaata tgcttttggt
    19801 cgaatgcttt tattttagac gcttttgggt aaaaatttgt ttatcttccc aacggagcat
    19861 ggttaaatgg cttccccaag cacagccggt atcaagtgca tagatgtttg gacgagtgca
    19921 tttgcccatt aggcttgccc agtgaccaaa gataatgttt ttctgtgtaa ataacggatt
    19981 atctaagtca aaccaagcca gcagttcttg aggggcttgt tcaagcggta atttacagtc
    20041 aaaatctaac cgcttgtcgg tgtagcaaaa ccgcatacgg gtaaacacat tgatgatata
    20101 acgccaacgt tcaacacctt gcaaatcttc actccagcgt tcgggcttat tctcatacat
    20161 ttgggcgatg tagtccgcat aattgtcact ttgtagcacc ttttctgcct ctttggcaca
    20221 agctagggta gttgctaaat cccactcagg gctgattcct gcgtgagtca gcaaaaattg
    20281 atgtttgggg tgatgaatta caagcggttg atttctgagc caattttgca aatcgagccg
    20341 atctttagcc tcaaaaatag catcaatctt atcatttggt tttacccgtt tgatgccaag
    20401 caagtgcaaa tcgtggttgc ctagcacggt tttggcattg tttgcagagt ctttcacaaa
    20461 acgtaaacag gctaaagagt cctcaccacg ggcgactaaa tcccctgtta aaaagagttc
    20521 gtcttgcttg gggttataat tgactttttc taataaaagc tgaaattcgt taaagcaacc
    20581 gtgtaggtcg cctacaatat aagttgccat tgtttctcct ttgttcccct aaagttataa
    20641 acaagcggtt atattttaga gaaaatttgc aaaatctcat caaaatataa ccgcttgaaa
    20701 tttaaatgat atttcctacg attgagcggg tttctaagcg tacttcggtg agtttcactt
    20761 tcaccgcttg cccgatctga taggcttttt cacctttaat ataaagtgca agttcttctt
    20821 ggcggtattc catttcctct ttgttgtcgt gcaagctgga gaatggcaca aaaatttgag
    20881 caccgttttt tatgactttc gcacgcaagc caccacggga aacatctgca atttcgcact
    20941 caaattcgac cgcttgttcc accattgggg caaggtaacg agcataaagc cagtcagcaa
    21001 tatcacgttc tactaaacgg ttttgacggc gagcttcttg taaacggctt aaaactgatt
    21061 cttccaccgc ttggctttgt ttgccaagta acacttgctt gattaaacgg tggtttacca
    21121 tatcgccata tttacggatg ggtgatgtcc aagtggcgta atcggtaatg cctagtccga
    21181 aatgcggggc aacttcggct ttaaattcag cgaaggttaa ataacgtcgt aaacgcaact
    21241 ctaagaattt ctctgggtag tcttgaatat cacgttgcat ttggcaatag ccttctaagg
    21301 ttgctaaacg ctcggcggag tagcgttcgg caagctcatc acgattttca tcgctggcta
    21361 aagtttggag taaaaatgat tttaccaatt cataattttt cggatcaaaa cctgaatggg
    21421 tattaaatac accggttttg gcatgttggc ttaagaattt tgcacaacaa atattggcaa
    21481 gaatcattga ttcttcgatc atttgattcg caatacggcg atattcaacg tgaatatctt
    21541 ttaccgagcc atcttcattt aactcaaagg tataatcgcc ttgttctttg aatagcaacg
    21601 cattctcggc acgccattga atgcgggctt gagtaaattg atgtagccac tcaatttgtt
    21661 gttttgtttc ttcagactga ggttgccacg cattttccac gccttcgaga taatcggaaa
    21721 catcgtcgta agccaagcga tcttttgatt cgacccaagc agaggtaaag gttgtctctt
    21781 taatgagatt gccgtttaaa tcggtttcaa taaagccaac gagggcaggg cgtttttcat
    21841 tcgggacaag cgaacagaga tcgtctgaga gttctcgagg taacattgga atgttaaagc
    21901 ccggcaaata gttggtgaaa caacgctggc gagcggcttt ttcaatatgg gaattttctg
    21961 ggatataagc ggttgggtca gcaatcgcta ccactaaacg ccagccggtt cgcttgccat
    22021 tttgcttaat tggttcgatg aacagagcat cgtccatatc ttgcgtgctt gggctgtcaa
    22081 tagtggtaaa gtaaagatga gataaatctt cacgagctaa ttcatcgtgt aattcatagt
    22141 ttttttcatt tgcgaccggt tcacgaggct gttcgtggcg ggcaagtgtg acccaccaag
    22201 gggcaaaatt atcagattct ttacagataa attgggtaac ttgagcaaag aaaaaacggt
    22261 cgtcacgcaa tgggtgcgtt tttaattgtg caaccaccca gtcgccattt tctaattttt
    22321 cagtggcttt tttatgggta tttgccggga tagtttgctt aatgcttggg tgatcaaccg
    22381 caagttgcaa tttgccctct ttattcaagc gaacttgggc aataaatcta tccagcattg
    22441 gctcaagtag tgaatcaatt tctacctgtt ctttgtcgcc atcacgtttg actacggctt
    22501 tcactgaatc accgtgcatt acttttttca tttccgcagg tggaataaaa tagctttttt
    22561 tatcgcattc taaaaaaccg aatgctttgt ctgaggcttt gacaacgcct gcgacatatt
    22621 ctttgcttgc ttcaatttgt tgtttgagtt gggctaaaag gggattgttt tgaaacataa
    22681 tttctcattt taaaaataaa aaaaggtgct atcgttgcac cgaaaaaaac aatggcatta
    22741 taacggattt gaaccaagtt cagcaaataa atgctgttgc tatcacttat aaaggttggt
    22801 agaatataat ccatttaaaa ataatggaga cttttcaatg aaaaaattac ttgcaattgc
    22861 aacagtattt tctttagcag cctgttctac tcaaacactt gaactatctc ctgctaagga
    22921 agctcaatat gatcgtgttc aacacttctt tgtttcaggg cttgctcaaa aacaagagat
    22981 agatgcagca aaaatttgta atggtgcaga taaggtaggt aaggtagaaa ctgaaactac
    23041 tttctttaat ggcttgttag gtacaattac ttatggtatc tattcacctc gccaaatccg
    23101 tgtatattgt aactaatcct agtaatttaa ggagttgaaa atgaaaaaat tgcttgctat
    23161 gtctgtatta acagccctgc tgactacagc atgtacaaca caaactgccc atattggcgg
    23221 caaagttact gataatatta aaccaactaa gagtgtaact cagtcgttct ttataggtgg
    23281 gattgggcaa gaagaaacat tgaatgttgt agaggtttgt ggttccaaag ataaagtgca
    23341 gcaggttgaa acagtacttt caggtgggga cattctatta ggtcttgtga ctatcggtat
    23401 ttatacacct cgtactgcaa atgtatattg taagtaa
//
LOCUS       DANLUQ010000020        22769 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.020, whole genome shotgun sequence.
ACCESSION   DANLUQ010000020 DANLUQ010000000
VERSION     DANLUQ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 22769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22769
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.020"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            59..1165
                     /gene="mltA"
                     /locus_tag="PXB27_000656"
     CDS             59..1165
                     /gene="mltA"
                     /locus_tag="PXB27_000656"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HDL5345477.1"
                     /translation="MNWKAYKKAIAAASVVAIATVLVACSSSGQKGKTTSYDAEKAKF
                     GAKYNGRHFMPYGTHFQAGRLGLNAAPVNQLDFLQQLSNVRAYSSLADSYAIAYGKIS
                     RWISSGGNINDLAKYGISTRLMRGEDGYQNVLMTGYYSPVIKARRTPQGQYQHPIYAM
                     PSSKRFSRAEIYAGALQGQGLELAYSNSMLDNFLLGVQGSGYVDFGDGRLNYFAYAGQ
                     NGFKYASVGRLLVEDGEIPKEKMSVQAIREWGQRNPSRVQGLLERNPSYVFFKNDPTG
                     QVKGSAGVPLVPLASVASDRSIVPSGSVLLVEMPLIDSKGNWTGKHEMRLMVALDVGG
                     AVKGHHFDLYQGIGDKAGHIAGLMKHYGRVWVLN"
     gene            1178..1627
                     /locus_tag="PXB27_000657"
     CDS             1178..1627
                     /locus_tag="PXB27_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345478.1"
                     /translation="MLKKLSAIFLLLPSAVLANNLQNITASTEPKYKISEIDVRILIR
                     QLNNIEQCIYPELAKPGYQQIYANWNLAENLTMQYFEYQLLKELLGEENQKLMQNDTL
                     STEYFHLLHSRLNHQKANVDPEKCDTFKPRYKEIYKSMENALTKKNQ"
     gene            complement(1661..3178)
                     /gene="purF"
                     /locus_tag="PXB27_000658"
     CDS             complement(1661..3178)
                     /gene="purF"
                     /locus_tag="PXB27_000658"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDL5345479.1"
                     /translation="MCGIVGIIGNSPVNQAIYDGLTLLQHRGQDAAGIVTIDDENRFR
                     LRKANGLVSDVFQQEHMVRLQGNVGIGHVRYPTAGSSSVSEAQPFYVNSPFGITLVHN
                     GNLTNNAELKARLYNEARRHVNTNSDSESLLNIFAYFLDLYSTQHLSPDNIFETVRKT
                     NDSIRGAYACIAMIIGHGMVAFRDPFGIRPLVLGKREIEGKTEYMFASESVALDVVGF
                     EFVRDVLPGEAIYVTFDGQLHSQICADNPKLNPCIFEYVYFARPDSVIDGVSVYSARV
                     HMGELLGEKIKREWGRIIDDIDVVIPIPETSNDIAVRIANMLYKPYRQGFVKNRYVAR
                     TFIMPGQAQRKSSVRRKLNAIASEFKGKSVLLVDDSIVRGTTSEQIVEMARAAGAKRV
                     YFASAAPEIRYPNVYGIDMPTCEELVAYDRSVEEVAQMIGVDKLIFQDLEALYKSIQL
                     ENPTIHRFDDSVFTGEYITGDVDKCYLDSIARSRNDKAKAEAAKQATNLEIHNER"
     gene            complement(3193..3696)
                     /locus_tag="PXB27_000659"
     CDS             complement(3193..3696)
                     /locus_tag="PXB27_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HDL5345480.1"
                     /translation="MVDLIVIGIIAFSVLISLWRGFTNEALSLLGWVIAFFVASSFYP
                     YLSGYLTQINSVYLQNSEFLRNGIAAAILFILTLIVCGVINGLLGKVIDLSGMSITDR
                     ILGGAFGALRGILIVSAILFFLDTFTQASQTELWKSSLLIPHFDFIVKWFFEQLQANS
                     SFLNTTK"
     gene            complement(3842..4303)
                     /gene="rnhA"
                     /locus_tag="PXB27_000660"
     CDS             complement(3842..4303)
                     /gene="rnhA"
                     /locus_tag="PXB27_000660"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HDL5345481.1"
                     /translation="MKLVEIFTDGSCLGNPGKGGIGILLRYNGYEKTVSKGYFQTTNN
                     RMELRAVIEALAMLKEPCKVQLNSDSQYMKNGIQKWIFNWKKNDWKTSDKKPVKNKDL
                     WVALDQEIQRHQIEWSWVKGHSGHRENEICDELAKQGANNPTLDDVGYIAD"
     gene            complement(4396..5727)
                     /gene="argG"
                     /locus_tag="PXB27_000661"
     CDS             complement(4396..5727)
                     /gene="argG"
                     /locus_tag="PXB27_000661"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005722481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HDL5345482.1"
                     /translation="MSATILEALPLGQKVGIAFSGGLDTSAALLWMRKKGAVPYAYTA
                     NLGQPDEDDYNAIPKKAMEYGAENARLIDCRQQLAHEGIAAIQCGAFHISTGGIPYFN
                     TTPLGRAVTGTMLVAAMKEDDVNIWGDGSTFKGNDIERFYRYGLLTNPKLKIYKPWLD
                     QLFIDELGGRLEMSKFLIENGFDYKMSVEKAYSTDSNMLGATHEAKDLEELSTGMKIV
                     KPIMGVAFWDESVEIKAETVSVTFEEGVPVALNGKRIDDAVELIMEANRIGGRHGLGM
                     SDQIENRIIEAKSRGIYEAPGMALLHIAYERLVTGIHNEDTIEQYRINGLRLGRLLYQ
                     GRWFDPQALMLRETAQRWVAKAITGTVTLELRRGNDFTILNTESPNLTYEAERLSMEK
                     VEDAPFTPADRIGQLTMRNLDIVDTRGKLAVYTDAGLLAANNSVIPQLGKK"
     gene            5911..6951
                     /locus_tag="PXB27_000662"
     CDS             5911..6951
                     /locus_tag="PXB27_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDL5345483.1"
                     /translation="MKKLLVIRNDKIGDFMVCFPAFAMLKQSMPNVEITALVPSYTAP
                     LAELCPSIDKIIIDTANKKDKNEFNRILQAVKNEQFDAVICFVSDWHNAKLTWQSGIK
                     YRLAPATKLFQFLYNHRLTQRRSQSAKAESEYNLDLARAFLSKHNIPIVEPHTPYLSV
                     EQQTVQAQKAKLCEQLNITTVKKWVFVHSSTGGSANNLSIEQYAELIQGILAEFDCYV
                     VLTAGKGESEKAQQLAQQINHNNVVVYDKNEGLQDFTHSLACADLFIAGSTGPLHISG
                     ALNIPTIGFYPSRLSAIPRRWKPINDANKHIAFCPPSDKASQMNLTLISISEALKTIV
                     PFIRRMWNTPSL"
     gene            complement(7016..7774)
                     /locus_tag="PXB27_000663"
     CDS             complement(7016..7774)
                     /locus_tag="PXB27_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL5345484.1"
                     /translation="MPTLSVAMIVKNEAQDLRACLDTVKDWVDEIVILDSGSTDETPQ
                     IAAEYNARFYTNTDWQGFGKQRQLAQQYVTSDYVLWLDADERVTAELKQSILQAVKND
                     EKNTVYKISRLSEVFGKQIRHSGWYPDCVVRLYRTEFAKYGDELVHEKVHYPKEAKIQ
                     KLQGDLLHFTYKDIHHYLVKSASYAKAWAIQRANAGKTASLFDGVTHAIGCFVKMYLL
                     KAGFLDGKQGFLLAVLSAHSTFVKYADLWNRTRK"
     gene            7901..8215
                     /gene="yhbY"
                     /locus_tag="PXB27_000664"
     CDS             7901..8215
                     /gene="yhbY"
                     /locus_tag="PXB27_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010786380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDL5345485.1"
                     /translation="MSITLTTKQKQFLKGLAHHLSPVVMLGGNGLTEGVLAEIDNALD
                     HHELIKVKVAGADRETKQLIIDAILRETKAAEVQTIGHILVLYRQSEEKKISLPKATK
                     AI"
     gene            8276..8998
                     /locus_tag="PXB27_000665"
     CDS             8276..8998
                     /locus_tag="PXB27_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDL5345486.1"
                     /translation="MFKKFSLYTLAMLLIPVVTWLANWHWSLAIQYAEFDFDYFLYLI
                     TETGSVPYAIITCVVFALLLARVVRKRYSLIFVITVCVASMVATQAIKTAMKSTFQEP
                     RPFVTALFQDKTDDFYQLTREQRGLAVMEKIGANQNFVTAHQAKEVGYSFPSGHTIFA
                     VSWLLLVVGFCRNMRGQAVVLAHFFAISWAALMLISRLRLGMHYPIDLFTSSLIAFLI
                     NLALFVWFVPWLESKKWFAKKP"
     gene            9067..9228
                     /locus_tag="PXB27_000666"
     CDS             9067..9228
                     /locus_tag="PXB27_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345487.1"
                     /translation="MEMSIEKIAELLSDKEKDDYPFSIEERQFAAQASADTWEAILNN
                     TIQPFDFKK"
     gene            9236..9598
                     /locus_tag="PXB27_000667"
     CDS             9236..9598
                     /locus_tag="PXB27_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345488.1"
                     /translation="MYQVFIAEPKDPNLEKGLLKHFIKSYLTQEELDFLHKILTNLPN
                     IRPFWGDTGYKYIVIDNVYFLYEVSESETKVILLGTKFNNKRKTFNFANTKTQQKSMK
                     KWINDLAIYKPHKINQSQ"
     gene            9611..10528
                     /gene="nagK"
                     /locus_tag="PXB27_000668"
     CDS             9611..10528
                     /gene="nagK"
                     /locus_tag="PXB27_000668"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005708433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HDL5345489.1"
                     /translation="MAIYYGLDVGGTKIELAAFNEKLEKLHSERVPTPQTNYQDWLTA
                     IKTLVETADTKFDEKGTVGLGIPGFVNRETGIAEITNIRVADGKTILKDLAECLGREV
                     RAENDANCFALSEAWDESNKPYSTVLGLIIGTGFGGGFVFDGKIHSGRIGMAGEVGHM
                     QLNYHALKLLDWDKAPIYECGCGNQACLDTYISGRGFELLFNDLVGEKISAKAIIERF
                     YQQETKVVAFVEKYIELMAISISNLITILDPDMIVFGGGLSNFDYIYQALPKALPKHL
                     MRSAKVPVIKKAIHGDSGGTRGAAALFLK"
     gene            complement(10599..12548)
                     /locus_tag="PXB27_000669"
     CDS             complement(10599..12548)
                     /locus_tag="PXB27_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL5345490.1"
                     /translation="MYQRSQSRTVLFPILLFVGINLVILTLSRLVLGVWQSDRVSAVD
                     GWIPLILQGIRIDISSLCWLFGLPALFSVLCFHKNVLGSIWQTVLRIWLTVGSVFILF
                     MEVATPNFIETFDLRPNRLFIEYLVHPREVFTMLINGHLWAVICGIGIAMISLIIYWK
                     LSGWATKNLHFPSWKMRPLLALLVAAIVFVGGRSSFAHRGINPAMVAFSSDPLVNSLV
                     LNSGYSVLYAVQQLKDEDKSSEQYGKMSVEEMFNIVKQVRNRPASDYISDEIPTLTQN
                     KATYQGKPKNIVIILEESLGAQFVGSLGGKNLTPNLDKLSEQGWYFTNLYATGTRSVR
                     GIEAVTAGFTPTPARSVVKLTKSQTNFFSIAELLKRQGYHTSFIYGGEKHFDNMASFF
                     YGNGFQQIIDEKDYKNPKFTATWGVSDEDLFDKANETFNQLHKSGKPFFSLVFSSSNH
                     DPFEFPDGKIELYEQPKQTRHNAAKYADYAIGHFFELAKKSEYWQDTVFLVIADHDSR
                     AVGEHLVPIQHFHIPALLLGEHIQPRTDNRLVSQLDMPTTLLSVAGISGQYPMIGYDL
                     TQDIDPNRAFMQYDATQAMMRGNDVVVMRPNLPIEGFSYDKANYKLTPKEQPESFKKE
                     ALAHALLGSYLYKNQLYKLPEETKQ"
     gene            complement(12581..13369)
                     /locus_tag="PXB27_000670"
     CDS             complement(12581..13369)
                     /locus_tag="PXB27_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HDL5345491.1"
                     /translation="MQVTELNIYPIKSTQAIQVSQAFVEPKGLNFDREFMLAEPNGKF
                     ITARKDPELYRLAAFPIATGVMITHTSGQKCVALYQDFVEEQSSEVWGTHFNAKVAAE
                     AVNQWLSEIFGRAVQLRWLGSQSQRIVANFADHPMSFADSNPVSLMSLKSLEQVQQWS
                     PIPLEMARFRPNIVIDGNVAFEEEQWQQVQIGEVLFTKAALCTRCILITRDLNTLELD
                     PKSEPFRTLKEKHCDEKGKPVFGIHLVPQNSGVIKVGDPVIIQL"
     gene            13494..13724
                     /gene="xseB"
                     /locus_tag="PXB27_000671"
     CDS             13494..13724
                     /gene="xseB"
                     /locus_tag="PXB27_000671"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDL5345492.1"
                     /translation="MAKKPTQDFESTLKELEAIVTHLESGDLPLEEALNEFETAIKLV
                     QQGQERLQKAEQRIQILLNKNDTAPLSDYERE"
     gene            13730..14623
                     /gene="ispA"
                     /locus_tag="PXB27_000672"
     CDS             13730..14623
                     /gene="ispA"
                     /locus_tag="PXB27_000672"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HDL5345493.1"
                     /translation="MSYLLATDLTHLQQRINHFLAEKLKTTDSVGSPLVEAMAYAVLL
                     GGKRVRPFLIYATGRMLGVPLEKLDHSAAAMEAIHAYSLVHDDLPAMDDDRLRRGKPT
                     CHIAFDEATAILAGDALQSFAFELLATDPHLTDREKVVQVTELATASGAKGMCLGQSL
                     DLIAENKAVSLEELELIHRNKTGALILSSVMMGLNLSEYSQNVAIKQPLVNYAKAIGL
                     AFQVQDDILDVIGETDKIGKIVGSDEHLNKSTYPKLLGLDGAKQKAEMLYQTALHSLE
                     QLPFDTTALKELANFIVKREN"
     gene            complement(14658..15458)
                     /locus_tag="PXB27_000673"
     CDS             complement(14658..15458)
                     /locus_tag="PXB27_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HDL5345494.1"
                     /translation="MCQLLGMNCNTPTDITFSFDGFRRRGGLTDHHTDGFGIAFFEGK
                     GVRVFRDNRPGASSPMAEAISQYQIKSLNVIAHIRKATRGDVNLENTHPFIRELWGEN
                     WVFAHNGTVEGVGVCEECHYQPIGTTDSEAAFCRIVSELKEMYPAKPSEKEIFDAVVQ
                     ITSRIAEHGVFNFVLSNGHWMIARCTTNLHYVCRKAPFGKALRDDDVSIDFSQYTKIT
                     DKVTIIATQPLTKNENWTKMKNGGYVFFKDGDVVYEIEGTLPEAQPHV"
     gene            complement(15583..16368)
                     /gene="znuB"
                     /locus_tag="PXB27_000674"
     CDS             complement(15583..16368)
                     /gene="znuB"
                     /locus_tag="PXB27_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005724066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HDL5345495.1"
                     /translation="MFDILFPAWLAGVLLAFITAPLGAFVVWRKMAYFGDTLSHSALL
                     GVALGIFLELDPYLAVIGMTILLALLLVWLEQKTNYSVDTVLGIIAHTSLSLGVITIS
                     LLDNVRVDLMSYLFGDLLAINFSDVVFIGIGATLICLILMLFWKRLLSITISPELAQI
                     EGLNVARLRLLLMILTALTIALSMKFVGALIITSLLIIPSATARRFAKTPEKMVLIAI
                     FFSIIAVSGGLLLSSWKDTPAGPSVVICAGLIFLLSLFKKEYH"
     gene            complement(16455..17792)
                     /locus_tag="PXB27_000675"
     CDS             complement(16455..17792)
                     /locus_tag="PXB27_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDL5345496.1"
                     /translation="MSSLSFDFSEDFRPLAAKMRPRNLAEYVGQSHLIGEGKPLRRAI
                     EAGHSHSMIFWGPPGTGKTTLAEIIAHHLDAEVERISAVTSGVKEIREVIEQAKLNRQ
                     AGRRTLLFVDEVHRFNKSQQDAFLPHIEDGTIIFIGATTENPSFELNNALLSRARIYI
                     LKPLQAVEILKILQMAIADTERGLGNETLVLEDDVLALLADYVNGDARFALNCLELMV
                     DMAQDSAKGKVLNKSLLTEVLGERQARFDKGGDRYYDLISALHKSIRGSSADGALYWY
                     ARILTAGGDPLYVARRLLAIASEDIGNADPRAMQIAIVAWDCFTRVGAYEGERAIAQA
                     IIYLAVAPKSNAVYQAFNEAKRLAKEAKDYDVPAHLRNAPTQLMKSLGYGEEYRYAHN
                     EPNAYAAGENYFPPELKDTEFYFPSERGMEKQIKEKMAWLKAQDQASPIQRYK"
     gene            complement(17802..18290)
                     /gene="mreD"
                     /locus_tag="PXB27_000676"
     CDS             complement(17802..18290)
                     /gene="mreD"
                     /locus_tag="PXB27_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDL5345497.1"
                     /translation="MRTNPIFQILVIIAIFVVAFVLEIMPWPVGFQGLRPTWVVLVLI
                     YWALALPDKMSVGTAFIAGIVWDLILGSILGIHALVLSVAIYFVAKYHLILRNLSLWL
                     QSLLVMAYIVLIRFFIFVIEYILHSAEFNSQELLGAVISGVLWSWIFLLMRHIRRQLR
                     LR"
     gene            complement(18290..19345)
                     /gene="mreC"
                     /locus_tag="PXB27_000677"
     CDS             complement(18290..19345)
                     /gene="mreC"
                     /locus_tag="PXB27_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDL5345498.1"
                     /translation="MKPIFAKAPSLGMRLIIAVIFSIMLIISDGRSSLMIQARNLLET
                     AVSGLYYFANTPRSILDGVSGNFIDNNKLQLENNLLKEQLREKSADLLLLDQLKVENQ
                     RLRLLLSSPLRQDEYKKIAEVLAAEMDAYRQQVVINQGRLDGAFVGQPIIDERGVVGQ
                     VISVGEKSSRVLLLSDITHAIPVQVLRNDVRGIASGTGVNNELVIDNLPRSIDVVKGD
                     VLVTSGLGGRFPEGYPVAIVETVENDTKSQFARIVARPLASFDRLRYLLLLWPTAEEK
                     NYSQSLSPAQVREVVEERRNSVNPFERLKQMSNKKTEVQLEAKEEQIENDEINENPAA
                     QPEAENEQNYHTEEGAE"
     gene            complement(19447..20499)
                     /locus_tag="PXB27_000678"
     CDS             complement(19447..20499)
                     /locus_tag="PXB27_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HDL5345499.1"
                     /translation="MFKKFLGLFSNDLSIDLGTANTLVYVKGQGIVLDEPSVVAVRQD
                     RMGSLKSIAAVGSHAKLMLGRTPKSIMAIRPMKDGVIADFFVTEKMLQHFIKQVHSNN
                     FMRPSPRVLVCVPAGATQVERRAIKESAIGAGAREVYLIEEPMAAAIGAGLPVHEAMG
                     SMVIDIGGGTTEVAVLSLNGIVYSTSVRIGGDKLDEAIIAYVRRHFGSAIGEATAERI
                     KQEIASAKIESDDDVKTMEVHGHNLAEGAPRTFELNSRHVLEAIEQPLNGIVEAVKSV
                     LEQCPPELAADIFERGMVLTGGGALLHNLDRLLSDSTGVQVVVAEDPLTCVARGGGKA
                     LEMIDMHGGDVFSIDD"
     gene            complement(20614..21243)
                     /locus_tag="PXB27_000679"
     CDS             complement(20614..21243)
                     /locus_tag="PXB27_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HDL5345500.1"
                     /translation="MDIFIQGFIVCFGLIVSIGAQNAFLLKQGILKQHIFWIALLCFL
                     GDVFLMTIGVLGLGTLIAELPVVSFIISLLGAIFLLSYGSRSFISVFKSGEYLVASGE
                     NATSLKKALLITFAITFLNPHVYIDTVVIVGSIGGKLDFNGKIYFLAGALLCSFIWFF
                     GIGYGASLLSPYFAKRRTWQILDAVTGLIMYFIAFSLLIYTYQLALQIF"
     gene            21347..21994
                     /gene="udk"
                     /locus_tag="PXB27_000680"
     CDS             21347..21994
                     /gene="udk"
                     /locus_tag="PXB27_000680"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDL5345501.1"
                     /translation="MTDTSQTSSCVVIAIAGASASGKSLIASTIYKELKNELGTDNIG
                     IISEDAYYRDQSHLTMEERIKTNYDHPNSMDHHLLVEHLRQLKQGNAIEIPEYDYTEH
                     NRKTTTKHFEPKKIIILEGILLLTDENIRNEINVSIFVDAPLDICFIRRLQRDMVERG
                     RSMESVISQYRKTVRPMFLQFIEPSKQYADIIVPKGGKNRIAIDILKAQIKQLLK"
     gene            22009..22590
                     /gene="dcd"
                     /locus_tag="PXB27_000681"
     CDS             22009..22590
                     /gene="dcd"
                     /locus_tag="PXB27_000681"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HDL5345502.1"
                     /translation="MRLCDTDIKRYLDEGIIAITPRPSDDKISGATADVRLGNSFRVF
                     REHKTPYIDLSGPREEVAAQLNKVMSDEIIIGDDEAFFLHPGDLALATTLESVSLPAN
                     IVGWLDGRSSLARLGLMVHVTAHRIDPGWEGKIVLEFFNAGKLPLALRPNMAIGALSF
                     EILSGDAAKPYNARKDAKYKNQQSAISSRINQD"
     gene            22668..>22769
                     /locus_tag="PXB27_000682"
     CDS             22668..>22769
                     /locus_tag="PXB27_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009041538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HDL5345503.1"
                     /translation="MLHYAVVFFVIAIIAALLGFGGIAGSATEIAKIL"
BASE COUNT         7066 a         4885 c         4254 g         6564 t
ORIGIN      
        1 tgtttttgct attcttctgt ttatttttca cattttatta acgatataag gatattcgat
       61 gaattggaaa gcatataaaa aagctattgc ggcagctagc gtggtagcga ttgcaacggt
      121 attagtggcg tgttcgtctt caggccaaaa aggcaaaaca acctcttatg atgcggaaaa
      181 agctaaattt ggagcaaaat ataatggtcg tcactttatg ccttatggca cacattttca
      241 agctggacgt ttaggattaa atgctgctcc tgttaatcaa ctagactttt tacagcagct
      301 ttctaatgtc agagcctata gtagcttggc agattcttat gctatcgctt acggaaaaat
      361 cagtcgttgg atctcatcgg gcggtaatat taatgattta gcaaaatatg gtattagtac
      421 tcgtttaatg cgtggcgaag atggctacca gaatgtgtta atgacagggt attactcgcc
      481 ggttatcaaa gcccgtagaa cgcctcaggg gcaataccaa cacccgattt atgcgatgcc
      541 gtctagcaaa cgtttttcac gggcggaaat ttacgcagga gcattgcaag gtcagggctt
      601 agaactggct tatagtaatt caatgctcga taacttctta ttaggtgttc aaggtagcgg
      661 ttacgttgac tttggtgatg gtcgcttaaa ctattttgct tatgcagggc aaaatggctt
      721 taaatatgcc agcgttgggc gtttattagt agaagatggc gaaattccga aagagaaaat
      781 gtcagtgcag gctatccgtg aatgggggca acgtaaccct agccgtgttc aaggtttgtt
      841 ggagcgtaat ccatcgtatg ttttctttaa aaacgatcct accggtcagg taaaaggctc
      901 tgccggtgtg ccgttagtac cacttgcatc tgttgcatca gatcgtagca ttgtgccatc
      961 aggtagcgtg ttgttggtcg aaatgccgtt gattgatagc aaaggaaatt ggaccggcaa
     1021 acacgaaatg cgtttaatgg tggctcttga tgttggcggt gcagtgaaag gccatcactt
     1081 cgatttatat caagggattg gcgataaagc aggccatatc gcaggtttaa tgaaacatta
     1141 cggccgtgtt tgggtattaa attaatacag ggtaactatg ttaaaaaaac tatctgccat
     1201 attcttactt ctaccgagcg ccgtgcttgc aaataatttg caaaatataa ccgcttcaac
     1261 tgagcctaaa tataaaatct cagaaataga tgtcagaatt ttgatacgcc agctcaataa
     1321 tattgagcaa tgtatctatc ctgagttagc taaacccggt taccagcaaa tctacgcaaa
     1381 ttggaatctg gctgaaaatt taacgatgca atattttgaa tatcagcttc taaaagagtt
     1441 gctcggagag gaaaatcaga aactgatgca aaatgatacg ctttctaccg agtatttcca
     1501 tttactgcat tctcgtttaa atcaccaaaa agcaaatgta gatcccgaaa aatgcgatac
     1561 atttaaacct agatataaag agatttataa aagtatggaa aatgcactca ctaagaaaaa
     1621 tcaatagtaa aaaactcgca gttttcactg cgagtttctt ttatctttcg ttatgaattt
     1681 ctaaattggt ggcttgtttt gccgcctctg cttttgcttt atcgtttcga gatcttgcta
     1741 tactgtctaa atagcattta tctacatcac ctgtaatata ttctcctgta aatacagagt
     1801 catcaaagcg atgaatagtc ggattttcca gttgaataga cttataaagt gcttcaaggt
     1861 cttggaaaat caatttatcc acccctatca tctgtgcaac ctcttccact gagcgatcat
     1921 aagccactaa ttcttcacaa gtcggcatat caatgccata cacattcggg tagcgaattt
     1981 ccggtgcggc agaggcaaaa taaacccgtt ttgcaccagc tgctcgtgcc atttccacga
     2041 tttgttcaga cgttgtacct cgtacaatag aatcatcaac cagtaacacg cttttgcctt
     2101 taaattcaga ggcaatcgca tttaatttac ggcgaaccga gcttttacgc tgtgcttgcc
     2161 ccggcataat aaaagttcga gctacatagc ggtttttaac aaacccttga cgatagggtt
     2221 tatacaacat attagcaata cgtaccgcaa tatcatttga ggtttcagga atcgggatca
     2281 ccacatcaat atcatcgata attcgtcccc attcacgttt aattttctca cctaataatt
     2341 cgcccatatg cactcgtgca gaatatacag aaacgccatc aatgacggaa tcaggacggg
     2401 caaaataaac atattcaaaa atacaaggat tcagttttgg attatcggca caaatttgcg
     2461 aatgtaattg cccatcaaag gtaacataaa tcgcttcacc cggcagcaca tctcgcacaa
     2521 attcaaaccc cactacatca agagccacac tttccgaagc aaacatatat tcggttttac
     2581 cctcgatttc acgtttaccc agcactaacg ggcgaatacc gaatgggtca cggaaagcaa
     2641 ccataccgtg tccgataatc atcgcaatgc aagcataagc accacgaatg ctatcattgg
     2701 ttttacgaac cgtttcaaag atattgtctg ggcttaaatg ctgagtggaa tagagatcta
     2761 aaaagtaagc aaaaatatta agaagggatt cagaatcaga attagtgttc acatggcggc
     2821 gggcttcgtt gtataagcga gctttaagtt ccgcattatt agttaaatta ccgttgtgaa
     2881 ctaaggtaat accgaaaggt gaattgacat aaaatggctg ggcttcagac acacttgagc
     2941 tacctgctgt tgggtagcga acgtgaccaa ttccaacatt gccttgtaat ctcaccatat
     3001 gctcttgctg gaaaacatcg ctgactaagc cgttagcttt gcgtaagcgg aaacgatttt
     3061 catcgtctat ggtgacgata cctgcggcat cttgtcctcg gtgttgaagt aatgttaaac
     3121 catcataaat cgcctgattc accggcgaat tcccaataat accgacaatg ccgcacatat
     3181 agaaaaacct ctttatttag tcgtgtttaa aaaactggaa tttgcttgta actgttcaaa
     3241 gaaccacttc acaataaagt caaaatgcgg aattaatagc gatgatttcc ataattccgt
     3301 ttggcttgct tgggtaaagg tatctaagaa aaataaaata gccgacacaa ttaaaatacc
     3361 acgcaatgcc ccaaaagcac cgcctaaaat acggtcggtg atagacattc cgctcaaatc
     3421 aatcactttg cctaataaac cgttgatcac accacaaaca attaaagtca aaatgaacag
     3481 aatcgccgct gcaataccat tgcgtaaaaa ttctgaattt tgcaaataaa ccgaattaat
     3541 ttgtgtgaga taaccgctta aataaggata aaacgagctt gccacaaaaa aggcgattac
     3601 ccaaccaagt agcgataatg cttcattggt aaaacctcgc cataaactga taagcactga
     3661 aaaagcaatg attccgataa caattaaatc aaccataaca caccttaaag cgtaaagctc
     3721 tcctattcta caaaaaaaga aaacgtttgc gtagcactat ttcgctaaaa ataagtgctt
     3781 tatgcaaata caagcggtta aattcggtaa aaaatttaca caatccgacc gcttgtaaaa
     3841 cctagtctgc aatataacca acatcgtcca acgttggatt atttgctcct tgtttagcca
     3901 gctcatcgca aatctcattt tcacgatgtc ctgaatgccc tttcacccaa ctccattcaa
     3961 tttggtggcg ttgaatttct tggtctaaag caacccataa atctttattt ttaactggct
     4021 ttttatcgct ggttttccag tcatttttct tccagttaaa aatccatttt tggatcccgt
     4081 ttttcatata ttggctgtca ctattgagtt gcaccttaca aggctctttt agcattgcta
     4141 aagcctcaat caccgcccgc aattccattc ggttattggt ggtttgaaaa tagcctttgc
     4201 tcacggtttt ttcgtacccg ttataacgca gcaaaatacc aatcccccct ttgccggggt
     4261 tgcctaaaca agaaccgtct gtaaaaattt caactaattt catcatcacg ctttgaaaat
     4321 aaaagcccca aagcattaac tttggggcag cttttaggct aaattctaaa agttatcaga
     4381 atggagatca actgattatt tttttccaag ttgtggaatc acgctattat tagccgccaa
     4441 taaacctgca tcggtataaa ctgctaattt accacgggta tcgacaatat ctaagttacg
     4501 cattgttaat tgaccgatac gatctgccgg cgtaaacggt gcatcttcta ctttttccat
     4561 acttaaacgt tctgcttcgt aggttaagtt tggcgattca gtgttaagaa tagtgaaatc
     4621 gttaccacgg cgtaactcaa gcgtgaccgt accggtaatc gcttttgcca cccaacgttg
     4681 agccgtttca cgcaacatta aagcttgcgg gtcaaaccag cggccttgat acaataaacg
     4741 ccctaaacgt aatccgttaa tgcgatattg ctcaatcgta tcttcgttgt gaataccggt
     4801 aactaaacgc tcgtaagcga tgtgtaataa tgccattccc ggtgcttcat aaataccacg
     4861 agatttcgct tcgataatac ggttttcgat ttggtctgac attcccaaac catgtctgcc
     4921 accaatgcgg ttagcctcca taattaattc aacggcatca tcaatacgtt taccgtttaa
     4981 cgccaccggc acaccttctt caaaggtaac agaaacagtt tccgctttaa tttcaactga
     5041 ttcgtcccag aatgccacgc ccataatcgg ttttacgatc ttcataccgg tgcttaattc
     5101 ttctaaatct ttcgcttcgt gggttgcacc taacatattc gagtctgtag agtaggcttt
     5161 ttccaccgac attttatagt caaaaccgtt ttcgattaag aatttagaca tttccaagcg
     5221 accgcctaat tcatcaatga atagttggtc taaccacggc ttataaattt ttaattttgg
     5281 attggttaat aaaccgtaac gatagaaacg ctcaatatcg ttacctttaa aggttgaacc
     5341 atcgccccag atattcacat catcttcttt cattgccgca accaacattg tgccggttac
     5401 cgcacgacca agtggcgtgg tgttgaaata cggaataccg ccggttgaaa tatggaaagc
     5461 accgcattga attgcggcaa tgccttcgtg agctaactgt tggcgacagt cgattaatcg
     5521 tgcattttca gccccatatt ccatcgcttt tttcggaatt gcgttgtaat catcttcatc
     5581 cggctgacct aaatttgcgg tgtacgcata aggcactgcc ccttttttac gcatccataa
     5641 cagtgctgct gaggtatcta aaccgcctga aaaggcgata cccactttct gccctaaagg
     5701 taaagcttct aaaattgttg ctgacatacg aattcctata aattttgaaa gtaaaaatat
     5761 tcaaacgcaa acgcttacgt tcaagcaaaa tattctagct gaaaagtagt aaaaattcat
     5821 ctaaaattta gcccgatgat ttgcaaattt tttgtgaaat ttgaccgctt ataatttact
     5881 gaaaacctaa aagcaataga gaaaatcaga atgaaaaaac tactcgtaat ccgtaatgat
     5941 aaaattgggg actttatggt ctgctttccg gcatttgcta tgttgaagca atcaatgccg
     6001 aatgtggaaa ttaccgctct tgtgccaagt tatactgctc cacttgccga gctttgccct
     6061 tctattgata agattattat tgatacggcg aataaaaaag ataaaaatga atttaaccgt
     6121 attttacaag cggttaaaaa tgagcaattt gatgcggtta tttgttttgt ttcagactgg
     6181 cataatgcca aactcacctg gcaaagtggg attaaatacc gcctcgctcc agcaactaaa
     6241 ttattccaat ttttatataa ccaccgttta acccaacgcc gttctcaatc agcaaaagcg
     6301 gaatccgaat ataatttgga tttagcccgt gcttttttaa gcaaacacaa tataccgatt
     6361 gttgaacctc acacgccata cttaagtgtt gaacagcaaa ccgtccaggc acaaaaagca
     6421 aaattatgcg aacaactcaa tattaccacc gttaaaaagt gggtatttgt gcatagttca
     6481 accggtggtt cggcaaataa tttatctata gaacagtatg cagagttaat tcaaggtatt
     6541 ttggcagaat ttgattgcta tgtggtactc accgcaggga aaggcgaaag cgaaaaagct
     6601 cagcagttag ctcaacaaat caatcacaac aacgtggtgg tttatgataa aaatgagggg
     6661 ttacaagatt ttacacattc gcttgcgtgt gcagatttgt ttattgccgg ctcaaccggt
     6721 ccgttacata ttagtggggc gttaaatatt ccaactattg gcttctaccc gagccgactt
     6781 tctgccattc cacgccgttg gaaaccgatt aatgatgcga ataaacacat tgcattttgc
     6841 ccaccgtctg ataaagcctc acaaatgaat ttaactttaa tttctatttc tgaggcatta
     6901 aaaacgatag taccgtttat tcggagaatg tggaatactc cctctctctg ataaaattgc
     6961 taaacgcaat tttatctatc tctctcccac tagagggaga gagtactgaa aagcatcatt
     7021 tccgagtacg attccacaaa tccgcatatt ttacgaaagt agaatgtgcc gaaagaactg
     7081 ccaataaaaa tccttgcttg ccatctaaaa aaccggcttt taatagatac attttcacaa
     7141 aacagccgat ggcgtgagtc acgccatcaa ataagctggc tgttttgcct gcatttgccc
     7201 gttggattgc ccacgcttta gcgtaacttg ccgattttac caaatagtgg tgaatatctt
     7261 tataggtgaa atgcagcaaa tcgccttgta gtttctggat ttttgcctct tttgggtaat
     7321 gtactttttc gtgaaccaat tcatcaccat atttggcaaa ttccgtgcga tacaatctca
     7381 ccacacaatc cggataccaa cccgagtggc gaatttgttt gccaaatact tcacttaagc
     7441 ggctgatttt gtaaacggta tttttttcat catttttgac cgcttgtaaa atagattgct
     7501 tcagttcggc tgtcactcgc tcatctgcat caagccaaag tacataatca ctggtcacat
     7561 attgttgagc taactgacgc tgtttaccaa agccttgcca atcggtgtta gtgtagaacc
     7621 ttgcgttgta ttcagcagca atttgtggtg tttcatcagt actgccacta tctaaaatca
     7681 caatttcatc tacccaatct tttactgtgt caaggcaagc ccgtaaatct tgagcttcat
     7741 ttttgacaat catcgcaaca cttaatgtcg gcatagttta tccttctctc tttttgtcgt
     7801 gtgtttttct gctattcgtt gcattttact ttaaaaatag tggtttttgt gctattttct
     7861 acgccaaatt ttatcaacaa aacaaattaa cggaaaaaga atgtcaatta cattaacaac
     7921 caaacaaaaa caattcctaa aaggcttggc tcatcattta agcccagtgg tgatgctggg
     7981 gggtaacggc ttaacagaag gcgtgttagc tgaaatcgat aacgccttag accaccacga
     8041 actgattaaa gttaaagttg ccggggctga tcgtgaaacc aaacaattaa ttattgatgc
     8101 cattctgcgt gaaactaaag cggctgaagt acaaaccatt gggcacattt tagtgcttta
     8161 ccgccaaagc gaagagaaaa aaatcagctt gccaaaagca actaaagcga tctaattgca
     8221 aaaatatgtt taaattcaac cgcttgtatt caagcggttt tttataggac tttctatgtt
     8281 taaaaaattc tctctttata cccttgcaat gttgctcatt cctgtggtga cttggctggc
     8341 aaattggcac tggtctctgg caattcaata cgctgaattt gatttcgact actttttata
     8401 tttaatcacc gaaaccggct ccgtacctta tgccatcatc acctgtgtgg ttttcgcctt
     8461 attactggcg agggtggttc gcaaacgcta ctctttaatt tttgtgatta ccgtgtgtgt
     8521 cgcttcaatg gtagccactc aagcaatcaa aacagcgatg aaatcaacct ttcaagagcc
     8581 tcgtccattt gtcactgctc ttttccaaga taaaacagac gatttttatc aattaacacg
     8641 cgagcaacgt gggcttgccg taatggaaaa aatcggtgca aatcaaaatt ttgttaccgc
     8701 tcaccaagcc aaagaagtgg gctattcttt cccatcaggc cacactattt ttgcggtttc
     8761 ttggctatta ttggtggtag gtttctgccg taatatgcga ggacaagcgg tcgttttggc
     8821 tcattttttt gcaatcagtt gggcggcgtt aatgctgatt agccgcttgc gtttaggtat
     8881 gcactacccg attgatttat tcaccagtag tttaattgcg tttctaatca atttagcctt
     8941 atttgtgtgg tttgtgcctt ggttggaaag taaaaaatgg tttgctaaaa agccttaatt
     9001 ggcaatttag ccaaatcaat atacaataca aattattgaa taagtaccta aaataaggag
     9061 cttagaatgg aaatgtcgat agaaaaaata gctgaactat taagcgataa agaaaaagat
     9121 gactatccgt ttagtattga ggaaagacaa tttgccgccc aggctagtgc tgatacttgg
     9181 gaggcaatct taaataacac tattcaacct tttgacttca aaaaataggc ttgctatgta
     9241 tcaagttttt attgcagaac caaaagaccc aaacttagag aaaggcttat tgaaacactt
     9301 cattaagtct tatcttactc aagaagaact cgatttcctg cataaaattc tcactaattt
     9361 acccaacatc agaccttttt ggggcgatac cggttacaaa tatatcgtca ttgataatgt
     9421 ttacttcttg tatgaagttt ccgaaagtga aacgaaagtc attctacttg gcaccaaatt
     9481 taataataaa cggaaaactt ttaattttgc gaatacgaaa acccagcaaa aatcaatgaa
     9541 gaaatggatt aatgatttag caatttataa accgcataaa atcaatcaat cgcaataaga
     9601 gagcgtaatt atggcaattt attatggctt agatgtgggc ggcacaaaaa ttgaattagc
     9661 cgcttttaat gaaaaactag aaaaactaca cagcgaacgt gtgccgacac cgcagacaaa
     9721 ttatcaagat tggctgacgg caattaaaac gctggttgag actgccgata caaaatttga
     9781 cgaaaaaggc actgtcgggc tgggcattcc gggctttgta aaccgtgaaa ccggcattgc
     9841 ggaaattacc aatattcgag tggctgacgg caaaacgatt ctgaaagatt tagccgaatg
     9901 tttagggcgt gaagtgcgtg ccgaaaacga tgctaactgc tttgccctgt ctgaagcgtg
     9961 ggacgaaagt aataagcctt attccaccgt attagggttg attatcggca ccggttttgg
    10021 cggtggtttt gtgtttgacg gtaaaattca ttcaggcaga atcggaatgg caggcgaagt
    10081 tgggcatatg caacttaact accacgcact taaattgctc gactgggata aagccccaat
    10141 ttatgaatgt ggttgtggca accaagcctg tttagacacc tatatttcag ggcggggttt
    10201 tgagttactg tttaatgatt tagtaggaga aaaaatctcg gcgaaagcga ttatcgaacg
    10261 cttctaccaa caagaaacca aagtggtggc atttgtcgaa aaatatattg agctaatggc
    10321 aatcagtatc agcaatctga ttacgatttt agatccggat atgattgtat tcggtggcgg
    10381 tttatccaac tttgattata tctaccaagc tctaccaaaa gcattaccaa aacacttaat
    10441 gcgttcagcg aaagtgccgg tcatcaaaaa agcaattcac ggcgactcag gtggcactcg
    10501 tggtgcagca gcattattct taaagtaatg aacaaatttg agagagggca agcggtcatt
    10561 tttaataaaa atattgcagc cctctctttt aatttgcctt actgcttagt ttcttccggt
    10621 aatttatata actgattttt atataaataa ctgccaagca atgcgtgtgc taacgcctct
    10681 tttttaaacg attccggctg ttctttaggg gtgagtttat agttagcttt atcgtagcta
    10741 aatccttcta tcggtaaatt tggtcgcatt accaccacat catttccccg catcatcgcc
    10801 tgtgtcgcat catattgcat aaaggcacgg tttgggtcaa tgtcttgcgt taaatcatac
    10861 cctatcatcg gatattgacc acttattccc gccacagaaa gtaatgttgt cggcatatcg
    10921 agctgactca ctaaacggtt atccgttctt ggctgaatgt gttcgcctaa taaaagtgcc
    10981 ggaatatgga agtgttgaat tggcactaaa tgctcgccaa cggctcttga atcgtgatct
    11041 gcaatcacta aaaacaccgt atcttgccag tactctgatt ttttcgctaa ctcaaagaaa
    11101 tgcccgatag cataatcggc atattttgcc gcattatggc gagtctgctt cggttgttca
    11161 tagagttcaa ttttgccgtc cggaaattca aacggatcgt ggttactgga actgaatacc
    11221 aaactaaaaa acggtttacc tgatttgtgc agttgattaa aagtttcatt tgccttatca
    11281 aacagatctt catcactcac gccccaagtt gcggtaaatt tagggttttt ataatctttt
    11341 tcatcgataa tttgctggaa accattaccg tagaaaaagc tcgccatatt atcaaaatgc
    11401 ttctccccac cataaataaa ggaagtatgg tatccttgac gttttaataa ttcggcaata
    11461 ctaaaaaagt tagtttggct tttcgtcagt tttaccacag atcttgccgg tgttggtgta
    11521 aaaccagccg ttaccgcttc aattcctctc accgaacgag tacctgttgc ataaagattg
    11581 gtaaaatacc agccttgttc tgaaagttta tctaaatttg gagtgagatt tttccctcct
    11641 agcgaaccga caaattgagc ccctaaactt tcttctaaga taatcacaat gtttttaggc
    11701 tttccttgat aagtcgcttt attttgcgtg agggtgggaa tttcatctga aatataatct
    11761 gaagctggtc gattacgaac ctgcttaaca atgttgaaca tttcctccac agacattttt
    11821 ccatactgtt cggaagattt atcttcatct ttcaattgct gaaccgcata aagcacggaa
    11881 taacctgaat ttagcaccag tgaattgaca agcggatcgc ttgaaaatgc caccattgcc
    11941 ggattaatcc ctcgatgggc aaaactggaa cgcccaccaa caaaaactat tgcagcaacc
    12001 aataaagcca gtagcggacg cattttccag ctcgggaaat gcaaattttt ggttgcccaa
    12061 cccgataatt tccaataaat gatgagagaa atcatcgcaa tgccaatccc acaaataaca
    12121 gcccataaat gtccgttaat cagcattgtg aacacttctc tcggatggac caaatactca
    12181 atgaaaagac gatttgggcg aaggtcaaag gtttcaataa aattcggtgt ggcgacttcc
    12241 ataaaaagaa taaatacact accgacagtt agccaaattc ttaaaacagt ctgccaaatc
    12301 gaacccagta catttttatg aaaacacaac acgctaaata atgccggtaa gccaaatagc
    12361 caacatagcg aagatatatc aatgcgaata ccttgtagta ttaatggtat ccatccatca
    12421 acagcggata ctcgatcaga ttgccaaacg cctaacacta aacgggataa ggtgaggata
    12481 actaaattaa tcccaacaaa tagtaggatt ggaaatagca cagttcttga ttgactgcgt
    12541 tggtacattt tgaatctcct tttaaaaaaa tatcggcata ttataattga ataatgacag
    12601 gatcgcctac ttttatcaca ccactgtttt gtggcacaag gtgaataccg aacaccggct
    12661 tacctttttc atcgcaatgc ttttctttta aggttctaaa cggctccgat ttggggtcta
    12721 actcaagcgt gtttaaatcc cgtgtaatca aaatgcaacg agtacataat gccgctttgg
    12781 taaatagcac ttcgccaatt tgaacttgtt gccactgctc ttcttcaaat gccacattgc
    12841 catcaatcac aatattcggg cgaaaacgag ccatttcgag tggaatcggc gaccattgtt
    12901 gcacttgctc aagcgatttt agagacatca aactcaccgg gttgctatct gcaaaactca
    12961 ttgggtgatc ggcaaaattc gcaacaattc gttggctttg cgagccaagc caacgtagtt
    13021 gcacagccct accaaaaatt tcactcagcc attgattgac cgcttctgcc gccactttgg
    13081 cattaaaatg tgttccccac acttcactac tctgctcttc cacaaaatct tggtaaagtg
    13141 caacgcattt ttgaccgctt gtatgggtaa tcattacccc tgtcgcaatc gggaaagccg
    13201 ctagacggta taattccgga tctttacggg cagtaataaa tttgccgtta ggctcggcta
    13261 gcataaattc acggtcaaaa tttaagcctt tcggttcaac aaatgcctgt gaaacttgaa
    13321 tagcttgagt tgattttatc gggtaaatat tgagctcggt aacttgcata gaaaatcctc
    13381 catttggaaa taggataaca atacctgcaa gcggtcgaaa agtaaaagga atttgcaaat
    13441 cccttgtgag aacggtaaac ttgagcaact tttacagcaa taggtcagca acaatggcaa
    13501 agaaacccac tcaagatttt gaaagcacac ttaaagaatt agaagcaatc gttacccatt
    13561 tggaatccgg tgatttaccg cttgaagaag ccctcaacga atttgaaact gcaatcaaat
    13621 tagtgcaaca agggcaagaa cggttacaaa aagccgagca acgtattcaa atcttattaa
    13681 ataaaaacga tactgcccca cttagcgatt acgaaagaga ataatcacaa tgagctatct
    13741 attagcaacc gatctgactc acctgcaaca acggattaac cattttctcg ctgaaaaatt
    13801 aaaaacaacg gatagcgttg gttcgccttt agtagaagca atggcttatg cggtattgct
    13861 aggcggtaaa cgggtgcgac cgtttttaat ctatgccacc ggcagaatgc ttggtgtgcc
    13921 gttggaaaaa ttagatcata gtgcggcggc aatggaggct attcacgctt actcgctggt
    13981 tcacgatgat ttaccggcaa tggacgatga tagattacgc cgtggcaagc caacctgcca
    14041 tattgccttt gatgaagcta ccgctatttt agccggtgat gcgttacaaa gttttgcgtt
    14101 tgaactgctg gcaaccgatc cgcatttgac cgaccgagaa aaagttgttc aagttacaga
    14161 attggcaacc gcttccggtg caaaaggaat gtgcttaggg caaagtttgg atttgattgc
    14221 ggaaaataaa gcggtgagct tggaagagct tgaattaatt catcgcaaca aaaccggggc
    14281 attgattcta tcatccgtga tgatgggatt aaacctttcc gaatattcgc aaaatgtagc
    14341 cataaaacaa ccgcttgtaa actatgccaa agcgattggc ttagcctttc aggtgcaaga
    14401 tgatatttta gatgtcattg gcgaaacaga taaaatcggc aaaatagtgg gttcagatga
    14461 acacttaaat aaaagcacat acccgaaatt gttgggctta gacggggcaa aacaaaaagc
    14521 cgaaatgctt tatcaaaccg ctctccattc gctggaacag ttgcctttcg ataccaccgc
    14581 attaaaagag ttagctaatt ttattgttaa gcgtgaaaac taagaaaaaa cctagccgaa
    14641 gctaggtttt ttagtgatta aacgtgaggc tgagcttccg gcaatgtgcc ttcaatttca
    14701 tataccacat cgccgtcttt aaagaaaaca tagccaccgt ttttcatttt ggtccagttt
    14761 tcatttttgg taagtggctg agtggcaata atcgtcactt tatcggtaat tttggtatat
    14821 tgactaaaat caatcgacac atcatcatca cgcaaggctt tgccaaacgg tgctttacgg
    14881 caaacgtaat gcagattagt tgtacaacgg gcaatcatcc aatgaccgtt ggataaaaca
    14941 aaattaaata ccccgtgttc cgcaattctt gaggtgattt gcaccacggc atcaaaaatc
    15001 tctttttcgc ttggtttagc cggatacatc tcttttaatt ccgacacaat acggcaaaat
    15061 gccgcttcgg aatcggttgt accaatcggc tggtagtggc actcctcgca taccccaaca
    15121 ccttccactg tgccgttatg ggcaaacacc cagttctcgc cccataattc acgaatgaag
    15181 gggtgggtat tttccaaatt cacatcacca cgcgtagctt tacgaatatg tgcaataaca
    15241 ttgagcgatt taatttgata ttggctgatt gcttctgcca tcggcgatga tgctcccggg
    15301 cggttatcac gaaaaacccg aacccctttg ccttcaaaaa aggcaattcc aaacccatca
    15361 gtatggtgat ccgtcagccc accccgacgg cgaaatccat caaaagaaaa agtaatatcg
    15421 gttggcgtat tacaattcat tccaagtaat tgacacataa tttgcttctt aaaaccaaat
    15481 aatttacaac aaaaaataat tgttacattt aaacagattg attagaaaat tcaatgctct
    15541 ttacataaaa atcttatgct ttattttcat acattatgct gtttaatggt attctttttt
    15601 gaatagcgat aatagaaaaa tcaagccggc acaaatgact accgaaggcc ctgctggtgt
    15661 atctttccaa ctggataaca ataaaccgcc actgactgca ataatactga agaaaatcgc
    15721 aatcaacacc attttttcag gcgttttagc aaagcgtctt gccgtagcag acggaataat
    15781 cagcagtgag gtaataatta aagcaccgac aaatttcata cttaatgcga tagttaatgc
    15841 ggttaaaatc atcagcaata aacgtaagcg agccacattt aatccttcaa tttgagcaag
    15901 ttccgggctg atggtaatcg acaacaaccg cttccaaaac agcattaaaa tcaaacaaat
    15961 tagggttgct ccgattccga taaacaccac atcagaaaaa ttaattgcca gtaaatcgcc
    16021 gaataagtaa gacatcaaat ccactcgtac attatccaac aaactaatgg taattacccc
    16081 gagagaaagg ctagtatggg cgataatccc caacacggta tccaccgaat aattggtttt
    16141 ttgctccaac caaaccagca ataatgcaag aagaatagtc attccaatta cagcaagata
    16201 cggatcgagt tctaaaaaaa tccctaatgc cacgccgagc aatgcagagt gcgaaagtgt
    16261 gtcgccgaaa tacgccattt ttcgccacac cacaaaagcc cctagaggtg cggtaataaa
    16321 ggctaacaaa acaccggcaa gccaagccgg aaataaaata tcaaacatat tggttctcaa
    16381 aggcaagtaa aacaagcggt tattttttcg gaaaaatttg caaatttttt gctgatttag
    16441 accgcttgtc atatttattt ataacgttga atcgggctgg cttgatcctg tgctttcagc
    16501 cacgccattt tttctttaat ctgtttttcc attccccgct cacttgggaa gtagaattcg
    16561 gtatctttca gctcaggcgg gaaataattc tccccggcgg cataagcgtt tggctcattg
    16621 tgggcatagc gatactcttc gccatagcct aaagatttca ttaattgagt gggagcatta
    16681 cgcaaatgcg ccggcacatc gtagtctttt gcctctttag cgaggcgctt tgcttcatta
    16741 aaagcttgat agaccgcatt gctctttggg gcaactgcca aataaatgat cgcttgggca
    16801 atcgctcttt caccttcata agcccccact cgggtaaagc aatcccaagc cacaatcgca
    16861 atttgcattg ctcgagggtc ggcattacca atatcttcag aagcaatcgc aagcagcctt
    16921 cttgccacat aaagcggatc accaccggcg gttaaaattc gggcatacca gtataatgct
    16981 ccatcagccg atgagcctcg aatggattta tgtaacgcag aaatgagatc atagtaacga
    17041 tcaccgcctt tatcaaaacg agcttgtcgt tcgcctaaca cttcggtcag caaactttta
    17101 ttcagcactt tccctttagc tgaatcttgt gccatatcta ccattaattc caaacagttc
    17161 aaggcaaaac gggcatcacc attaacataa tcagcaagca atgccagtac atcatcttca
    17221 agtaccaaag tttcattgcc taaaccacgt tcggtatcgg ctatcgccat ttgcaaaatt
    17281 tttagaattt cgaccgcttg taacggcttt aaaatataaa ttctggctcg ggaaagcagt
    17341 gcgttattta gctcaaaaga gggattttcg gtagtcgcac caataaaaat aatagtgccg
    17401 tcctcaatat gcggtaaaaa cgcatcttgc tggcttttgt taaagcggtg aacttcatcc
    17461 acaaaaagca acgttcgtct gccggcttgg cggtttaatt tggcttgttc aatcacctct
    17521 cggatctctt ttacaccgct ggtcacagcg gaaatacgtt caacttccgc atcaagatga
    17581 tgggcgataa tttcagctag ggtcgttttt ccggtgccgg gcggtcccca aaaaatcata
    17641 gaatgactat gtccggcttc aatcgctctg cgtaacggtt tgccttcgcc aattaaatga
    17701 gattgcccga catattcagc caagtttcga ggtcgcattt tggctgcaag cggtcgaaaa
    17761 tcttcactaa aatcaaaaga caaactactc attttaacct cttaacgtaa ccgcagttgg
    17821 cgacgaatat gacgcattaa taagaaaatc caagaccaaa gcacaccact gattacagca
    17881 cctaacaatt cctgtgaatt aaattcggca ctgtgtaaaa tgtattcaat cacgaaaata
    17941 aagaaacgga tcagcacaat atatgccatt actaataggc tttgtagcca tagcgataag
    18001 tttcgcagaa tcagatggta tttcgcaaca aaataaatcg ccaccgataa cactaaggcg
    18061 tggatgccta aaatcgagcc aagaatcaga tcccagacga tgccggcaat aaaagccgtc
    18121 cccacactca ttttatcagg taatgccaat gcccaataaa ttaacaccaa aacgacccaa
    18181 gtagggcgta gcccttggaa tcctaccggc caaggcataa tctcaagtac aaaagcaacc
    18241 acaaaaatcg caattatcac aaggatctga aaaatcgggt ttgttctcat tattccgctc
    18301 cttcttccgt atgatagttt tgttcatttt ccgcttctgg ttgtgctgcc ggattctcat
    18361 ttatctcatc attctctatt tgttcttctt ttgcctctag ctgcacttca gttttcttgt
    18421 tagacatctg ttttaaacgt tcaaatggat tcacgctatt acgtctttct tcaacaactt
    18481 cacgtacttg cgctggcgac aagctttggg agtagttttt ctcttccgcc gtcggccaaa
    18541 gcagtaataa gtaacgtaag cgatcaaatg aggctaacgg acgagcaaca attctggcaa
    18601 attgactttt ggtatcattt tcaaccgttt ctacaatcgc caccggatat ccctcaggga
    18661 aacgtccacc taagcctgaa gtcaccagca catcaccttt taccacatca atagaacgag
    18721 gcaagttatc aatgaccaat tcattattca cccctgtacc actggcaatg ccacgtacat
    18781 cattgcgtag cacttgaaca ggaatagcat gagtaatatc agagagcaat aaaacacggc
    18841 tagatttttc ccctacagaa ataacttgtc caacaacacc tctttcatca ataataggtt
    18901 gcccgacaaa agcaccgtct aaacgccctt gattaatcac tacttgctga cgataagcat
    18961 ccatctcagc agctaatact tcagcaattt ttttatactc atcttgccga agcggagaac
    19021 tcaataacaa tcttaaacgc tgattttcaa cctttagctg atcaagcaat aataaatcgg
    19081 cacttttttc tcttagctgc tcttttaata aattattctc aagctgtaat ttattattat
    19141 caataaaatt gccacttacc ccatctaaaa ttgaacgggg ggtattagca aaataatata
    19201 atccacttac cgcggtttct aatagattac gcgcttgaat cattaaagag ctacgcccat
    19261 cggaaataat cagcataata gaaaatatca ctgcgataat caggcgcatc cctaaagagg
    19321 gagctttagc aaaaataggt ttcattagag aaatatatgt gaatttattg ggaatctata
    19381 acgaaaattg acaatagaat caatccattg ccaacctcca tttaaacagc tgctaagaga
    19441 aatgtattaa tcatcaatac taaatacatc accaccgtgc atatcgatca tttctaatgc
    19501 tttaccacca ccacgagcta cacaagttaa tggatcttct gctactacga cttgcacgcc
    19561 agttgaatca gaaagtaaac gatctaaatt atgtaataat gcaccacctc ctgttaacac
    19621 cataccgcgc tcaaaaatgt ctgctgccaa ttcaggtggg cattgctcta agactgattt
    19681 aactgcctct acaataccat ttaatggttg ttcaattgct tctaatacat gtcttgagtt
    19741 aagctcgaaa gtacgaggag cgccttcagc taaattgtgt ccgtgtactt ccattgtttt
    19801 aacatcatca tcggattcaa tcttagcact agcaatttct tgtttaattc gctctgccgt
    19861 tgcttcacca atagcagaac caaaatgacg acgtacataa gcaataatag cttcatctaa
    19921 cttatcacca ccaatacgca cagaagttga atacacaatg ccgtttaaag aaagcactgc
    19981 cacttcggtt gtaccgccac caatgtcaat taccattgac cccattgctt cgtgaaccgg
    20041 caaacctgca ccgattgcag ccgccattgg ctcttcaatt aaatagactt cacgcgctcc
    20101 tgctccaatt gcagattctt taattgcacg gcgttccact tgtgttgcac ctgctggcac
    20161 gcacactaat acacgagggc ttgggcgcat aaagttatta ctatgtactt gtttaataaa
    20221 gtgttgcaac attttttctg ttacaaaaaa atccgcaatg acaccatctt tcattggacg
    20281 aatcgccatt atgctttttg gtgtacggcc tagcattaat tttgcatgtg agcctaccgc
    20341 cgcaatactt tttagcgatc ccatacgatc ttgacgtaca gcaaccactg agggttcatc
    20401 tagcacaata ccttgacctt taacataaac cagtgtattt gctgtcccga gatcgataga
    20461 aagatcgttt gaaaataatc ctaaaaattt tttaaacata aaagaatccg ttaaaataaa
    20521 attattcaaa aataagcaca ttttatagtg cgaacattgc tactaatata gcaaaaaagc
    20581 ccgtcattca tcaatgaaaa aatttaaact ccactagaaa atttgcagag caagttgata
    20641 agtataaatc agtaaactaa aagcgataaa gtacataatt agcccggtaa cagcatctaa
    20701 aatttgccaa gttcttcgct ttgcaaaata aggggaaagt aagcttgctc cataacctat
    20761 gccaaaaaac caaataaatg agcaaagtaa tgcacctgct aaaaagtata ttttaccgtt
    20821 aaaatctaat ttcccaccga tactgcctac aatcacaacc gtatcaatat aaacgtgagg
    20881 atttaagaat gtaatcgcaa aggtaatcag taaagccttt tttaagctcg ttgcattctc
    20941 tccacttgcc acaaggtatt caccactttt aaatacgctg ataaatgaac ggctaccgta
    21001 gcttaataga aaaatggccc ctaaaagcga aataatgaaa cttacaacag gcaattccgc
    21061 aattaacgtg cctaagccta gcacacctat cgtcattaaa aatacatcac ctaaaaaaca
    21121 gagcaatgca atccaaaata tgtgttgttt taaaatccct tgctttaata aaaaggcatt
    21181 ttgtgcgcca atagagacaa ttaagccaaa acacactata aatccttgaa tgaaaatatc
    21241 cattttttct ccaaaatcgc ataacaagcg gtcaattata caaaaaattt tgcaaaactt
    21301 cggtataatt tatcgaatac tcttttgact gaataggaaa ttttttatga ctgatactag
    21361 ccagacttca tcttgcgttg tcattgcgat tgccggcgca tctgcctcag gtaagagcct
    21421 gattgcctca accatctata aagaactcaa aaatgaattg ggaacagata atatcggtat
    21481 tatttccgaa gatgcttatt atagagatca atctcatcta acaatggaag aacggattaa
    21541 aaccaattac gatcacccta attcaatgga tcatcattta ttggttgaac atttacgcca
    21601 attaaaacaa ggtaatgcga ttgaaattcc agaatatgac tataccgaac ataatcgtaa
    21661 aacgaccacg aagcactttg aaccaaagaa aatcattatt ttagaaggta ttttattgct
    21721 gacagatgag aatattcgca atgaaattaa tgtttctatt ttcgtagatg ctcctctgga
    21781 catctgcttt atccgccgct tacaacgtga tatggtggaa cgtggacgtt cgatggaatc
    21841 agtgattagt caatatcgca aaaccgttcg cccaatgttc ttacaattta tcgaaccctc
    21901 taaacaatat gctgatatta ttgtgccaaa aggtggcaaa aaccgtattg ccattgatat
    21961 tcttaaagca caaattaagc aattactaaa ataaaatagg agctaaatat gcgtttatgt
    22021 gatacagata ttaaacgtta cttagatgaa ggtattattg ccataacgcc tcgaccttca
    22081 gacgataaaa tttctggcgc cactgcagat gtgcgtttag gtaattcgtt tcgtgtattt
    22141 cgtgaacata aaacgcccta tattgattta agtggcccta gagaagaagt tgccgctcag
    22201 ttaaataaag tgatgagtga tgaaattatc atcggtgatg atgaagcatt tttccttcat
    22261 cccggtgacc ttgcactcgc aacaaccctt gaatcggttt ccttaccagc caatatcgtt
    22321 ggatggttag atggtcgttc gtcacttgct agattaggct taatggtaca cgttaccgcc
    22381 cacagaatcg atccgggttg ggaaggcaaa attgtattgg aattttttaa tgctggaaaa
    22441 ttacccctag ctttacgccc taatatggca attggcgcat taagctttga gattttaagt
    22501 ggtgatgctg caaaacccta taatgcgcga aaagatgcaa aatataagaa ccagcaaagt
    22561 gccatttcaa gtcggattaa tcaagattaa tatagtacat gccttgcaat catacattaa
    22621 ctatgttagt tttttaatca gtttattaca cagtaaggag tataactatg ttacattatg
    22681 cagtcgtatt ttttgtcatc gctataatcg cagcgctatt aggatttggt ggtattgctg
    22741 gaagtgctac tgaaattgcc aaaattctc
//
LOCUS       DANLUQ010000021        22294 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.021, whole genome shotgun sequence.
ACCESSION   DANLUQ010000021 DANLUQ010000000
VERSION     DANLUQ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 22294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22294
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.021"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            101..1753
                     /locus_tag="PXB27_000683"
     CDS             101..1753
                     /locus_tag="PXB27_000683"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HDL5345504.1"
                     /translation="MNGARFITESLKAHGVTTLFGYPGGAIMPTYDAIYDSGLDHLLC
                     RNEQGAAMAAIGYARATGKVGVCIATSGPGATNLITGLGDAALDSIPVVAITGQVASH
                     LIGTDAFQEADVLGLSLACTKHSFIVQNIEELPEILALAFQIAQSGRPGPVLVDIPRD
                     VQLAQTSALPIVYPKEPADAQDPNKLVVAKQLLATAKRPVLYVGGGVSMADGVQAVRN
                     FVKIANIPSVSTLKGLGTIDPTDPLYMGMIGMHGTKAANYAVQECDLLIACGARFDDR
                     VTGKLDTFAPHAKVIHIDIDNAEINKLRQAQVALKGNLIEAVNYLAQPLDIEPWREDV
                     RNLKQQFDFQYQENSGDGNIDAVALLHTLSQRKAKNSIVTTDVGQHQMWSAQHLTYHD
                     PKNYITSAGFGSMGFGLPAAIGAIKARAEDQVILITGDGSIMMNIQELGTLKRADLAV
                     KILLLDNQRLGMVRQWQSLFFHGRHSQTILDDNPDFVLLAKAFDIDGERIEKASEVSD
                     AIDRMFNAKGAYLLHVCIPQEDNVWPLVPPGACNADMLDDEV"
     gene            1763..1981
                     /gene="ilvM"
                     /locus_tag="PXB27_000684"
     CDS             1763..1981
                     /gene="ilvM"
                     /locus_tag="PXB27_000684"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HDL5345505.1"
                     /translation="MEEYQLTLKANKRPETLERILRVIRHRGFEVKCLNVESTDSAFN
                     LNFTLASKRPLSLLSHQLEKLFDIIEIN"
     gene            2194..2667
                     /gene="nrdG"
                     /locus_tag="PXB27_000685"
     CDS             2194..2667
                     /gene="nrdG"
                     /locus_tag="PXB27_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDL5345506.1"
                     /translation="MENLRFSSEQIVWQEVPNETSLAFLITGCPLGCKGCHSADSWKA
                     CLGKELSSEYLSERLKRYQGLISCVLFMGGEWEAEKLLPLLQLAKKQGLNTCLYSGLE
                     QHEIAETLLHELTYLKTGRWIAERGGLESLTTNQRFTDLRNNQNLNHLFIRGASI"
     gene            2664..4445
                     /gene="nrdD"
                     /locus_tag="PXB27_000686"
     CDS             2664..4445
                     /gene="nrdD"
                     /locus_tag="PXB27_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDL5345507.1"
                     /translation="MIRLQPTQLDGKLHFINQYIQAKNAADGSKMDANANVTQKNIAT
                     MEQELMKDFFVQINRAKVSEKIAKIFGKETACEYLRQIEAHEIYVHDETSLKPYCVSV
                     TMYPFLRDGLTKLGGESKAPKHLASFCGSFINFVFAVSSQFAGAVATVEFLTYFDYFA
                     RKDYGDNYLQTHRSDIENHLQQVVYSINQPAAARGYQSVFWNISVYDKFYFEAMFSRF
                     VFPDFEKPNWETTSKLQLFFMQWFNKERSKAILTFPVVTAAMLTEKGKCKDDEFANQM
                     AKELSQGNSFFIYQSDNPDSLASCCRLRNEISDHSFSYSLGAGGVATGSINVITINMN
                     RLVQDNRDLSEEVSKIHKYQYAYRKLMEEYLAAGMLPVYDAGFISLHKQFLTIGINGM
                     AEAAESQGLTVGYNPDYIDFVQSRLKIIFQANQAAGEKYGVKFNTEFVPAENLGVKNA
                     KWDKEDGYFVPRDCYNSYFYVVEDEEINALDKFLLHGRELIEWLDGGSALHLNLDEAL
                     TQTGYRSLLDTAAKTGCNYFCINVKITICNECSHIDKRTLHSCSKCGSDNIDYGTRVI
                     GYLKRVSAFSSARQKEHSLRFYHREAA"
     regulatory      4502..4598
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4655..5473
                     /gene="thiM"
                     /locus_tag="PXB27_000687"
     CDS             4655..5473
                     /gene="thiM"
                     /locus_tag="PXB27_000687"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDL5345508.1"
                     /translation="MTTSLPYHSHYLQKIRQNRPLVHNITNIVAANFSANGLLALGAS
                     PIMAAAIEEMESVPALSNAVVINIGTLIGKEVEAMELAGKTANRLGIPLILDPVGVGA
                     TPFRRQTVETLVDQIHFTAIRGNAGELATLAGVEWQAKGVDAGEGSHNVVEIAQQIAK
                     RYQTLVAISGETDVVSSGSHTALLHNGSPMFPKITASGCLLSAVCGAFLAVADKEHYF
                     NALIEACAAYAVAGEVAAQALKPTQYGQFAVNLLDTLGALTPETVEQYVRVEYV"
     gene            5466..6779
                     /locus_tag="PXB27_000688"
     CDS             5466..6779
                     /locus_tag="PXB27_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5345509.1"
                     /translation="MSKTHSNPTKVAVASMVGTAIEYFDNYIYTMAAVLVFNVQFFNS
                     DDPVSNQLMSLSVLALAFFTRPMGSILFGHFGDKYGRKQTLIASLLLMGLSTVAIGLL
                     PNYAAIGIWATVLLCLCRIGQGLGLGGEWGGAALVATEHAPKHKRAFFGIFPQMGAPI
                     GLLLANGAFYLVNLIYGEQTFLDWAWRIPFVASIVMIAIGLYIRIKISESPIFLKAEQ
                     QGKNRHTPLKTLFSQHLKPFMIGVLIATAGYVLFYILVAFTPIFVKSPVAVSQAGHAT
                     GLGLPVNLYTQYLLITSVIFGIAIFFSGIYSDKIGRRLLLLLATGATVVYGLTMPFFL
                     ENGTPTSIFIFLTIGMILIGLSFGPMAVILPELFPTEVRYTGASLAYTAAGILGASVF
                     TIIAVKINENFGLFGVGGYLSVASLLSFWALWQVHETKDLELEEI"
     regulatory      6915..7011
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7079..8608
                     /gene="thiE"
                     /locus_tag="PXB27_000689"
     CDS             7079..8608
                     /gene="thiE"
                     /locus_tag="PXB27_000689"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDL5345510.1"
                     /translation="MSNYSNSPYQPNVIWTVAGSDSCAGAGLQTDLHTFHDFNLVGCS
                     VVTSVTAQHPHGVLCVTPVDDHTFRQQFEALLVQGYPNAIKIGLLCSQAQVEILCEYI
                     QKIRAESSHYCYVVYDPVAVASSGQALSDSILLQIVQQKLYPLVDLITPNGTELALLS
                     DTEIQTFGDVKTASHKLFAQGIKAVLAKGGHFEWLGETVRDYLLTANEIYAFSHARQQ
                     SVNTHGTGCTYASAVGAMLATGFDLADAVTVATAYLQKGLSEKQGRGQTALSSLCHTG
                     YPDDIAFFPQTELISAPLKLPKEPFAPTEYQLGLYPVVDSVKWLERLILVGVKTLQIR
                     IKNRPLAEIEQEIAHSVALAKQHNVRLFVNDYWQLAMKYQAYGVHLGQEDLATADLNA
                     IQQSGLRLGVSTHCYFEIMRALAVKPSYIAFGHVFPTQTKVMPSQPQGLTNLAKYVAL
                     VAPLNIPTVAIGGINEQSIENVMHTGVGSAAVVSAVTQAKDWQQAVKNLAKFANGGRN
                     E"
     gene            8601..9773
                     /gene="cytX"
                     /locus_tag="PXB27_000690"
     CDS             8601..9773
                     /gene="cytX"
                     /locus_tag="PXB27_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HDL5345511.1"
                     /translation="MNRTLSTLNSGLIWAGAGISASAIVVGTWIAPLGWQQGLLAIVL
                     GHLLGGILFFAAGLIGAKTGKNAMQTVQISFGRGSVFFSLANVLQLVGWTAIMIYTGA
                     EISNALSLALWEYSAFSLWAIVLGLLIVLWLFTGSNQVGKFKLVTLIAMFLLTLWLSI
                     KVLNGQENFPVSGEMPFSTAVELATIMPLSWLPLVSDYTAKAKKPFAATLSATIGYLI
                     TSAWMYALGLGMVLFTGQTEIAPMLLMTGMSLIGIMVMILSTVTTTFLDAYSAGVSAG
                     NITAKFKETPTAILVTIIGTLLAISLPVTQYENFLLLIGSVFAPMIAVQIADFFVLKR
                     HQAQPNVDWVAAGLWLVGFVLYHLLSYHQVHLLLGLTIPVMLVIFLSTIFVRRFTK"
     gene            9812..10021
                     /locus_tag="PXB27_000691"
     CDS             9812..10021
                     /locus_tag="PXB27_000691"
                     /inference="COORDINATES: protein motif:HMM:NF015737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDL5345512.1"
                     /translation="MESLVLNNDWVVTPQAQLAYHTINGKADEERLNLLVARAGVRVA
                     KGFALNNGWNLQPYAEVNGIAEKGQ"
     gene            10029..10205
                     /locus_tag="PXB27_000692"
     CDS             10029..10205
                     /locus_tag="PXB27_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDL5345513.1"
                     /translation="MQVNQYQFGVAENKGRVQGSLGLSAGNGNHRLGLEASVASGKKL
                     KQPFNVLVNYRYQW"
     gene            complement(10246..12102)
                     /gene="sppA"
                     /locus_tag="PXB27_000693"
     CDS             complement(10246..12102)
                     /gene="sppA"
                     /locus_tag="PXB27_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDL5345514.1"
                     /translation="MLTILKAFYRIFRCIRECVINLFFILFILLLVPVVAFISSSGST
                     QKPVFNQGALRLNLDGYLADNREEFTDFYRLVQSELGSSEPFKISTFDVVQAISHAKN
                     DPKITGLVLDLQKLQSADFSSLDFIGNEINNFKQSGKPVIAVGEDYSQKQYYLASFAD
                     EIYLNKVGAVDLKGLNYSNTYFKTLLDKIEAEPHIFRVGTYKSAVEPFMRDDMSEEAK
                     QNARGWLNGTWQQTVATLAQNRNIEPQALDLSPANYIEKYKQAKGDDAQLALNQKWVS
                     QLVSSQESRDKLIAQFGKNSEGSYNQIEFLDYMTELNDRFAEVNAPKIAVVNVEGAIA
                     GGESDEMNVGSETLVKLLQQARDDKNVEGLILRINSPGGSAVASELIRQEVEAFQQAG
                     KPVVASMGGMAASGGYWIAATSDKIIASPNTLTGSIGIFGLAVTFEKTAKNLGVSEDG
                     IATSALAQQAGLKSLPQEQAEVLQIGIENGYDRFLELVSRGRKMPKEVVDKVAQGQVW
                     LGTDALKHGLVDELGNFNVAYNALSELINQKREAENQPKVEQFGLQWFAEQDDSLFGT
                     LARDFKAQLQFSVANWLDLPLLKQPQQQLETLSRFNDPKQMYLYCLNCGKID"
     gene            12205..12768
                     /locus_tag="PXB27_000694"
     CDS             12205..12768
                     /locus_tag="PXB27_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL5345515.1"
                     /translation="MQILDLLHHRRSNKKFGDKAPNAEQLEQILKAGLRVPDHGRLKP
                     YRFVVIEKSGMPQFGECLKSAVAEFDMGEERLKKAEKLANQAPMIIGVVATLDDSIAK
                     VPSWEQMITAGCATYAIQLAANAQGFDTVWITNKWINGSALRSAFGCQESDKIIALIM
                     LGSPAEGEQIALAPESESLEGFVRYLK"
     gene            complement(12839..15163)
                     /locus_tag="PXB27_000695"
     CDS             complement(12839..15163)
                     /locus_tag="PXB27_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDL5345516.1"
                     /translation="MTDLNLKISQIIAGELNVGSHQILAAITLLGEGNTIPFIARYRK
                     EVTGGLDDTQLRHFETRLIYLREMDERRQTILKSIEEQGKLTDELKAKILSSESKTEL
                     EDLYLPYKPKRRTRGQIAIEAGLEPLADSLWNDPKQDPETLAANFVDAEKGVADTKAA
                     LDGARYILMERFSEDAELLAKLRQYLTAYATLESKVIDGKEEEGEKFRDYFAHSEPFN
                     SVPSHRALAMFRGRNEGVLSLSLNADPTAEEGTKSSHCEEIIREHLGVIFNNQPADKW
                     REQVIAWTWKIKAALHLETELMATLCEKAEEEAIDVFARNLSALLMAAPAGARNTMGL
                     DPGLRTGVKVAVVDNTGKLLDTATIYPHTGREAEAQLAIFSLIKQHNVELIAIGNGTA
                     SRETERFAKAVIKEIKENKPQTVVVSEAGASVYSASEFAANEFPNLDVSLRGAVSIAR
                     RLQDPLAELVKIEPKAIGVGQYQHDVNQSQLARKLDAVVEDCVNAVGVDLNTASAPLL
                     ARVAGMTKTLAQNIVAFRDENGRFTSRSELKKVPRLGPKAFEQCAGFMRIIGGKNALD
                     ASSVHPEAYPVVEKILQATTSTLAELMGNNSKIHSLNAKDFVDEQFGLPTVNDIFKEL
                     EKPGRDPRGEFKTAVFMDGVEEITDLKAGMILEGTVTNVANFGAFVDIGVHQDGLVHI
                     SMLSNSFVEDPHTVVKAGDVVKVKVLEVDAARKRIALTMRLDDKPTDKKEPTSGRNSA
                     EICKNTQKNDRLQSNNRNAFGNNAFADALKGWKK"
     gene            complement(15163..15717)
                     /gene="pilW"
                     /locus_tag="PXB27_000696"
     CDS             complement(15163..15717)
                     /gene="pilW"
                     /locus_tag="PXB27_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HDL5345517.1"
                     /translation="MFAKFFPKMTACILIFLLAACSSLPPSSSEVEFNRSEAVKARIN
                     LALAYLEQNDFPKAKQNIDKALAHDEKDYLPYSVLAYYYQQTGDAENAEKAYQTALKL
                     SQNRPDVLNNYGTFLCKQGQFQQAYQQFEQAVKSKAPYYHQADSLENMVLCAKKEPNL
                     QKIAETLTELEKLDKPRANLLKEN"
     gene            15937..16188
                     /locus_tag="PXB27_000697"
     CDS             15937..16188
                     /locus_tag="PXB27_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5345518.1"
                     /translation="MKIVVFQRKALKQLRKIPTGAEIRRKCDDLAYFPYCANIKALTN
                     HQYDYRFRVGNYRIFFNIEGETMNIVSIEEVKKRDERTY"
     gene            16172..16519
                     /locus_tag="PXB27_000698"
     CDS             16172..16519
                     /locus_tag="PXB27_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015702293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL5345519.1"
                     /translation="MKELTNIQYLNNEEGKPAFAVLPIATLEWLKQKANIKTAIETGI
                     PSAVAGIALDNNYSALRAWREYLGLTQEEVAQRLGISQSAYSQHENAKNLRKTTRIKI
                     AYALGLNEEQLDF"
     gene            complement(16560..17720)
                     /gene="trmG/rlmN"
                     /locus_tag="PXB27_000699"
     CDS             complement(16560..17720)
                     /gene="trmG/rlmN"
                     /locus_tag="PXB27_000699"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HDL5345520.1"
                     /translation="MSELITNAGCGSAEQPKEKINLLNLNRQEMRELFAEMGEKPFRA
                     DQLMKWIYHFGEENFDNMSNINKVLREKLKRIAEIKAPEVAVEQRSADGTIKWAMQVG
                     DQQIETVYIPEDDRATLCVSSQVGCALACTFCSTAQQGFNRNLSVAEIIGQVWRASKI
                     IGNFGVTGVRPITNVVMMGMGEPLLNMNNVIPAMEIMLDDFAYGLSKRRVTLSTSGVV
                     PALDKMREQIDVALAISLHAPNDELRDELVPINKKYNIKMLMDSVNKYREVSNANHGK
                     VTIEYVMLDHVNDSTNHAHQLAEVLKNTPCKINLIPWNPFPEAPYGKSSNSRVDRFQK
                     TLMEYGFTVTVRKTRGDDIDAACGQLAGDVIDRTKRTLEKRKFGQGIAVKTH"
     gene            17980..18876
                     /locus_tag="PXB27_000700"
     CDS             17980..18876
                     /locus_tag="PXB27_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDL5345521.1"
                     /translation="MKLSTIKKALVLTFGLSAAMAMAEPFKVVTTFTVIQDIAQNVAG
                     DKATVESITKPGAEIHDYQPTPKDIVKAQKADLVLWNGMNLERWFERFFENVKGKPAV
                     VVTEGITPMPITEGEYKNLPNPHAWMSTDNALIYIENIRAALVKYDPQNADTYNANAK
                     AYAEKVKGIAEPLRQRLAFIPEQQRWLVTSEGAFSYLAKDYNLKELYLWAINAEEQGS
                     PKQVKKVIDGVKANKIPVVFSESTVSDKPAKQVAKETGALYGGVIYVDSLSEKDGPVP
                     TYLDLLKVTIGTIVEGFERSKK"
     gene            18889..19632
                     /locus_tag="PXB27_000701"
     CDS             18889..19632
                     /locus_tag="PXB27_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL5345522.1"
                     /translation="MTTVSASISVENLSVRYNNGHIALQDVSFHLENGTICALIGVNG
                     GGKSTLFKSLMGLVKPLTGSVKLSQLPIAKALKQNLVSYVPQSEEVDWQFPVSVYDVV
                     MMGRYGYMNFLRRPSQTDKLKVEQAMQRLDVLHLKDRQIGELSGGQKKRVFLARALAQ
                     ESKIILLDEPFTGVDVKTENAIVELLKQLKNEGHLILVSTHNLASVPSFCDQVLMVNR
                     TLLAKGKTEEIFNNQNLERVFGGLLHYQK"
     gene            complement(19689..20498)
                     /locus_tag="PXB27_000702"
     CDS             complement(19689..20498)
                     /locus_tag="PXB27_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL5345523.1"
                     /translation="MLNWILEPLQFEFMQIALITAILVAGVSAVLSCYLVLKGWSLMG
                     DAISHAVLPGVVISSLAGLPLSIGAFFSGLGCALSVGYLKENSRLKEDTVMGIVFSGM
                     FAFGILLFTAFNTGEHLTHILFGNLLGVTKSDLLQTLLISLFTFVVMVAKRKDFLLYC
                     FDVNQAKVAGLPVRLLHYGLLSLLALTIISAMQVVGVILVVAMLITPGITAYLLTKRF
                     DSMLLIALGVAIFSSVFGTLLSYHIDAATGPTIILVQAAIFFLSFAYSKIH"
     gene            complement(20491..21351)
                     /locus_tag="PXB27_000703"
     CDS             complement(20491..21351)
                     /locus_tag="PXB27_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL5345524.1"
                     /translation="MLDLLLEPFTYNYMQKAIFVSMGVGLVCAFLSAFLMLKGWSLIG
                     DALSHAVVPGVAIAYALKLPYAFGAFFSGILAALSILWVKSLTKIKEDAVIGFIFTTF
                     FALGMFIVSLNPTSVDVNSIVMGNILGIADDDLWQVVLIIAISLLGLILFWKDLLLVF
                     FDEHHALSVGLSPLRYKVIFFTLLSACVVVALQTVGAILVIAMVVTPGATAYLLTDRF
                     PRLVLIAMLIGTLTSGIGAYLSYFLDGATGAVIVCLQTLIFLFAFCFAPKYGLITQKR
                     KLHRVEVQNA"
     gene            complement(21425..22210)
                     /locus_tag="PXB27_000704"
     CDS             complement(21425..22210)
                     /locus_tag="PXB27_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HDL5345525.1"
                     /translation="MMKKITILGLGWLGLPLAEALQNKGWRVAGTKRTVGELAIECYP
                     LDLNALAVTSEIEQLLAADALVIALPLSQIRPENYGCGIQTLVSLAIEKGLKHLVFTS
                     STSVLPMKSGVFDENAVVDSANLLVKMENWLLSQPIHCDILRLAGLVGKKRHPVFYLA
                     GKQNLSGAGQPVNLVHLEDCIAAISLLLEQPNGQRIFHLCAEQHPSRKDYYSEMAKRF
                     GLADLHFSEENQPLVRLIKADKICQELGFRYYYPDPYQFKVDI"
BASE COUNT         6408 a         4947 c         4688 g         6251 t
ORIGIN      
        1 ctatttattt ttactgaaaa taaagtttaa aaaacaggga atttatgtta tctatattgc
       61 ctggttttaa aataataaaa atattaagga cacaacatcc atgaatggag caagatttat
      121 caccgagagt ttaaaagctc acggggtaac gacacttttt ggctatccgg gcggggctat
      181 tatgcctacc tatgatgcaa tttatgattc aggcttagat catttacttt gccgcaatga
      241 acaaggtgct gcaatggcag ctatcggtta tgcccgtgca acaggtaaag taggggtttg
      301 tattgcgacc tccggccctg gggcaactaa tttaatcacc ggtttaggcg atgccgcact
      361 tgattctatt cctgtggtag caattaccgg acaagttgcc agccatttaa tcggcactga
      421 tgccttccaa gaagctgatg tactaggttt gtcactcgct tgcactaaac acagttttat
      481 tgttcagaat attgaggagc tccctgaaat tttagccctc gcattccaaa ttgcacaaag
      541 tggcagacct ggtccggtgc tggtcgatat tccacgtgat gtgcaactag ctcaaacttc
      601 ggctctgcct atcgtttatc ctaaagaacc tgccgatgca caagatccca acaaattagt
      661 ggttgccaaa caattattag ccaccgccaa acgcccggta ctttatgtcg gcggtggcgt
      721 tagtatggct gatggtgtac aagcggtcag aaattttgta aaaattgcaa atattccgtc
      781 tgtttctacc ttaaaagggt taggcactat cgatccaacc gatccgctct atatgggaat
      841 gatcggtatg cacggcacaa aagcagcaaa ttacgcggta caagagtgtg atttactcat
      901 tgcctgtggt gcccgctttg atgaccgtgt taccggcaaa ttagacactt ttgcccctca
      961 cgctaaagtg attcacattg atatcgacaa tgcggaaatc aacaagctcc gtcaagcaca
     1021 agttgcctta aaagggaatt tgattgaagc cgtgaattac ttggctcaac cgttagatat
     1081 tgaaccatgg cgtgaagatg tgcgtaattt aaagcagcaa tttgactttc aatatcaaga
     1141 aaatagtggt gatggcaata ttgatgccgt ggcgttatta cacacgcttt cacagcggaa
     1201 agccaaaaac agcatcgtca ctaccgatgt agggcagcat caaatgtggt cggcacaaca
     1261 tttaacttat cacgatccga aaaactatat cacctctgcc ggttttggct ctatgggttt
     1321 tggcttgcct gcggcgattg gggcaatcaa agctcgagcg gaagatcagg ttattttaat
     1381 taccggtgat ggctcgatta tgatgaatat tcaagaactc ggcacgctta aaagagctga
     1441 tttagcggtc aaaatcctat tactggataa ccaacgttta ggtatggttc gccaatggca
     1501 atcgctgttc ttccacgggc gacacagcca aaccatttta gatgataacc ctgattttgt
     1561 gttgttggcg aaagcctttg atattgacgg tgagcgaatt gagaaagcga gcgaagtgtc
     1621 agatgcaata gacagaatgt tcaatgccaa aggggcttac ttacttcacg tttgtattcc
     1681 acaggaagat aacgtttggc cactggtacc gccgggggcg tgtaatgccg atatgttaga
     1741 cgatgaggta taggagaata agatggaaga atatcaatta acgcttaaag ctaacaaacg
     1801 ccctgaaacc ttagagcgta ttttacgggt tatacgtcac cgtggttttg aggtgaaatg
     1861 cttaaatgta gaatcaacag acagcgcctt taacctaaat tttacattag caagtaaacg
     1921 tccactctct cttttaagcc atcaattaga aaaactgttt gatatcattg aaatcaatta
     1981 atcagacttt tcattcgccg tgtaaaaaaa tacacggctt ttttatgctt aaaatttatc
     2041 attcgctatc cttttgattt tattcatttc ttcaaatttc aactcttttt tttcacttat
     2101 tgctctttat tttcccattc aacagatcta acatactata tatagtgtta aataatgtta
     2161 aaaaacacaa tatattgtgt taatgaatta gtaatggaaa atctcagatt cagctcggaa
     2221 caaattgttt ggcaagaagt gccgaatgag acctcacttg cttttctgat tacaggctgc
     2281 ccgcttggct gtaaaggttg ccatagtgcg gatagctgga aagcctgtct tgggaaagag
     2341 cttagcagtg aatatctctc tgaacgccta aaacgctacc aaggcttaat tagctgcgta
     2401 ttgtttatgg gcggagaatg ggaagcagaa aaattactgc cgctgttgca actggcaaaa
     2461 aaacaggggc taaacacctg tctttatagt ggtttggagc agcacgaaat cgccgagact
     2521 ttattacacg agctaaccta cttaaaaacc gggcgttgga ttgccgaacg aggaggattg
     2581 gaaagtttaa ccaccaacca acgctttact gatttacgca ataaccaaaa tcttaaccat
     2641 ctttttattc gaggagcttc aatatgattc gcttacaacc gacacaatta gacggaaaac
     2701 tacatttcat taaccaatat attcaagcca aaaatgccgc agacggttca aaaatggacg
     2761 ccaacgctaa cgtcacgcaa aaaaacatag cgacaatgga acaggagcta atgaaagatt
     2821 tttttgtgca aatcaaccga gcaaaagtga gtgaaaaaat tgcaaaaatt tttggaaaag
     2881 aaaccgcttg tgaatattta cgtcaaatcg aagcacacga aatttacgta cacgatgaaa
     2941 ccagcctaaa accctactgc gtttcagtca ctatgtaccc tttcttgcgt gatggtttga
     3001 ctaaactcgg cggcgaatcc aaagccccga aacatttagc ctcattctgc ggttctttta
     3061 tcaattttgt ctttgcagtc agttctcagt ttgccggtgc agtcgccacg gttgaatttc
     3121 tgacctattt tgactatttt gcccgcaaag attacggcga taattactta cagactcatc
     3181 gttcagatat tgaaaaccac ctgcagcagg ttgtgtatag catcaaccaa ccggcagccg
     3241 ctcgtggtta tcaaagcgtc ttttggaata tttcagtgta tgacaaattc tattttgaag
     3301 cgatgtttag ccgttttgtt tttcctgatt ttgagaaacc aaattgggaa accacttcaa
     3361 aattacaact tttctttatg caatggttca ataaagagcg aagcaaagcg attctcactt
     3421 tccctgttgt gaccgcagca atgcttaccg aaaagggcaa atgtaaagat gacgagtttg
     3481 ccaatcaaat ggcaaaagag ctatctcaag gcaattcatt ctttatttat caatcagata
     3541 atccggattc ccttgcttct tgctgccgct tgagaaatga aatcagcgat catagcttct
     3601 cctattcatt aggggctgga ggtgttgcga caggttcaat caatgtaatt actattaata
     3661 tgaaccgttt ggtgcaagat aaccgtgatt taagcgaaga agtcagcaag atccataaat
     3721 atcaatacgc ttaccgcaaa ctgatggaag agtatttagc tgccggtatg ttgccggttt
     3781 acgatgccgg ttttatttca ctgcataaac aattcctgac tattggcatt aacggaatgg
     3841 cagaagctgc tgaatcacaa ggtttaaccg taggttacaa tccagactat attgattttg
     3901 tgcaatctcg gttaaaaatt attttccaag ctaaccaagc agccggtgaa aagtatgggg
     3961 taaaatttaa taccgaattt gtgcctgctg aaaatttagg ggtcaaaaac gccaaatggg
     4021 acaaagagga cggctatttc gtaccacgag attgctacaa ttcctatttc tatgtggtag
     4081 aagatgaaga aattaatgct ttggataaat tcttactgca cggcagagag ctgattgaat
     4141 ggctggacgg aggctccgct ctacacttga atttagacga agccttaacc caaaccggat
     4201 atcgctcctt gctggatacc gccgctaaaa ccggctgcaa ttatttttgt attaacgtta
     4261 aaatcacgat ttgcaacgaa tgtagccaca tcgacaaacg cacgttgcac agttgtagca
     4321 aatgtggctc cgataatatt gattacggca ctcgggtaat cggctattta aaacgagtct
     4381 ccgcctttag ttccgcccgc caaaaagagc acagcttgcg tttctatcac cgagaagcag
     4441 cttaattgaa aactgcagtt gtagcccttt acaaccgctt ttttagtgat tatattaaag
     4501 cctcatttag tcggggtgct tgcctaaggg caagctgaga aatacccgtt gaacctgatg
     4561 cagttagcac tgacgtagga aactaaaggc gtaaacccat ctattccccg ttggtgttgg
     4621 tgcgttgttt ttcaatcata ttaaggaata aacgatgacc acatcactcc cctatcactc
     4681 gcattatttg caaaaaattc gacaaaatcg accgcttgtt cacaacatta ccaacattgt
     4741 tgccgctaat ttttctgcca acggcttatt ggctcttggt gcttctccca ttatggcagc
     4801 tgctattgag gagatggaat ccgtgccggc actcagtaac gccgtggtga ttaatatcgg
     4861 cacgcttatc ggcaaagaag tagaagcaat ggagcttgct ggcaaaaccg ccaaccgttt
     4921 aggtattccg ctcattcttg accctgtcgg cgttggagca acacctttcc gccgtcaaac
     4981 agttgaaacc ttagtcgacc aaattcactt caccgccatt cgtggaaatg cgggggaact
     5041 agctaccctc gccggtgtag aatggcaagc caaaggcgtt gatgccggcg aaggctcgca
     5101 taatgtggtt gaaatcgccc aacagattgc caaacgttac caaaccttag tcgcaatcag
     5161 tggtgaaacc gatgtggtaa gctctggctc acacacagct ttattgcaca acggcagccc
     5221 gatgttcccc aaaattaccg cttcaggctg tttattaagt gcggtatgcg gggcattctt
     5281 agccgttgcc gacaaagagc actatttcaa cgcacttatt gaggcttgcg ccgcttatgc
     5341 cgttgcaggc gaagtggctg ctcaagcact caaaccaact caatacgggc aatttgccgt
     5401 caatctgctg gatacccttg gggcattaac gcctgaaacc gttgagcaat atgtgagggt
     5461 tgaatatgtc taaaactcac tcaaacccaa ctaaagtggc ggttgcctca atggtcggca
     5521 cggcgattga atattttgat aactacatct acacgatggc agcagtgctg gtgtttaatg
     5581 tgcagttttt taatagcgat gatccggttt ccaaccaatt aatgtcgctt tccgtactag
     5641 ctcttgcctt tttcacccgc ccgatggggt cgattttatt cgggcatttc ggcgataaat
     5701 acggacgcaa acaaactctg attgcctcgc tcttattaat gggcttatct accgtagcaa
     5761 tcgggctttt gcctaattat gccgccattg gcatttgggc aaccgtgtta ctctgcctct
     5821 gtagaatcgg acaaggttta ggcttaggcg gcgaatgggg cggagctgct ctcgttgcca
     5881 ctgaacacgc cccgaaacac aagcgggcat tttttggtat ttttccgcaa atgggcgccc
     5941 caatcggctt gctgttggca aacggagcgt tctacttggt gaatttaatt tacggtgaac
     6001 aaaccttctt ggattgggcg tggcgaattc cgtttgtcgc ttctattgtg atgatcgcca
     6061 ttgggcttta tatccgaatt aagatcagcg aaagcccgat tttccttaaa gcggaacaac
     6121 aaggtaaaaa ccgccatacc ccgcttaaaa ctttgtttag ccaacacctc aagcctttta
     6181 tgatcggcgt gctgattgcc accgccggct atgtgttgtt ctacatttta gttgctttca
     6241 ccccgatttt cgtcaaaagc ccggtggcag tttctcaagc aggacacgct acaggcttag
     6301 gtttaccggt aaatctctac acccaatatt tgctgattac ctccgtgatt ttcggcattg
     6361 cgattttctt ctccggcatt tattcagaca aaatcggacg ccgtttactg ctgttacttg
     6421 ccacaggagc aaccgtagtt tacggcttaa caatgccgtt tttcctcgaa aacggcacac
     6481 cgacttcgat ctttattttc ctcaccattg gaatgatctt aatcggctta agcttcggcc
     6541 cgatggcagt aattctaccc gaactcttcc caacagaagt acgttacaca ggggcttcgc
     6601 ttgcctacac cgctgccggt attttaggag ctagcgtgtt cactattatt gcggtcaaaa
     6661 tcaatgaaaa tttcggctta ttcggtgtag gcggctattt atcggttgcc tcattactga
     6721 gcttctgggc attgtggcaa gtccacgaaa ccaaagattt ggaattagag gagatataaa
     6781 aaaccactca cttaacgacg gggcaagtta ccaccttgcc ccaagatgag ggaatgcaaa
     6841 tttccctcta atagacttca caacggatat tttgcatttt tttataaaaa ataaccgctt
     6901 gtaagctata ctaaaaacgc ttgtcggagt gccgtaaggc tgagatcgca acagcgagat
     6961 ccgtggaacc tgtaggttag tacctgcgta gggaacaagt tttacccaaa ttcagatttt
     7021 gggatcatta ccacatctct ggcaagcaaa accctaagca tattatgagg attttgcgat
     7081 gtctaattat tcaaactctc cataccaacc taacgtcatt tggacggttg ccggctcaga
     7141 ttcctgtgcc ggtgccggtc tgcaaaccga tttacatacc tttcacgatt ttaacctagt
     7201 gggttgttca gtagttactt ctgtaacggc tcaacatccg cacggtgtgc tgtgtgtaac
     7261 gcctgttgat gatcacacct tccgccaaca gtttgaagcc ttgctggttc aaggctaccc
     7321 gaacgcaatc aaaatcggct tactctgctc gcaagcacaa gtagagatct tgtgtgaata
     7381 tatccaaaaa atccgtgcag aaagctcgca ttattgttat gtggtttacg accctgtggc
     7441 agtggcaagc agtgggcagg cgttatccga cagtatttta ttgcagatag tgcaacaaaa
     7501 attgtatccg ctggtggatt taatcactcc aaacggcact gaactcgctt tattaagcga
     7561 cacagaaatc cagacctttg gtgatgtgaa aaccgctagc cacaaactgt ttgcacaggg
     7621 aatcaaagcc gtgttagcca aaggtggaca ttttgaatgg ctgggggaaa cggtaagaga
     7681 ttatctgctg actgcaaatg aaatttatgc ctttagtcac gcccgacagc aaagtgtgaa
     7741 tacccatggc acaggttgca cctatgcttc agcggttgga gcgatgttgg caaccggctt
     7801 tgatttagct gatgccgtca ctgttgccac cgcttatttg caaaaaggct tatcggaaaa
     7861 acaaggcaga gggcaaactg ctttaagttc actttgccac accggctacc ctgatgatat
     7921 cgctttcttc ccacaaaccg agctgatttc tgcccctctt aaattgccga aagagccttt
     7981 tgctccaacg gaatatcaac ttgggcttta ccctgttgta gatagcgtta aatggcttga
     8041 acgcttaatt cttgtagggg taaaaacctt gcaaattaga atcaaaaatc gaccgcttgc
     8101 agagattgag caagaaattg cccattcggt cgcactggcg aaacagcaca atgtgcgttt
     8161 gtttgtaaat gattactggc agttagccat gaagtatcag gcttacggcg tgcatttagg
     8221 gcaggaggat ttagccaccg ccgatttaaa tgcaattcag caatctgggt tacggcttgg
     8281 tgtttcaacc cactgctact ttgaaataat gcgggcattg gcggtgaaac cgtcttatat
     8341 cgcttttggc cacgttttcc ctactcaaac caaagtaatg ccgtctcaac cacaaggctt
     8401 aacaaattta gccaaatatg ttgctctcgt tgcaccgctt aacattccaa ctgtggcgat
     8461 tggtggaatt aatgagcaaa gcattgaaaa tgtaatgcat accggtgtcg gcagtgccgc
     8521 ggtggtaagt gccgttaccc aagcaaaaga ttggcaacaa gcggtcaaaa acttagcaaa
     8581 atttgcaaat ggaggccgta atgaatagaa ctcttagtac gctaaacagt ggtttgattt
     8641 gggcgggagc gggcattagt gccagtgcaa tcgtggttgg cacttggatt gccccacttg
     8701 gctggcaaca aggcttgctg gcgatagtgc tagggcattt attaggcggc attttgtttt
     8761 ttgctgccgg cttaattggg gcgaaaaccg gcaaaaatgc gatgcaaacg gtgcaaattt
     8821 cctttggcag aggctcggtg tttttctccc ttgccaatgt gctacagctt gttggttgga
     8881 cagccattat gatctataca ggggcagaga tcagtaatgc ccttagcctt gccttatggg
     8941 aatattcggc tttctcgctt tgggcaatcg tgcttgggtt gctgattgtg ctatggttat
     9001 ttaccggctc taatcaggtc ggaaaattca agttagtgac attgatagcg atgtttttgc
     9061 tcaccttatg gctaagcatt aaggtattga atgggcagga aaattttcct gtttcgggtg
     9121 aaatgccgtt tagcaccgca gtggaattag caactattat gcctctttcg tggctacctt
     9181 tggtatcaga ctataccgct aaagcgaaaa agccttttgc cgcgaccctt tcggcgacca
     9241 taggctattt gatcaccagt gcgtggatgt atgcgttagg tctgggtatg gtgctattta
     9301 ccgggcaaac cgaaattgcc cctatgttac taatgacagg aatgagcctt atcggcatta
     9361 tggtgatgat tctttcgact gtgactacca cgtttcttga tgcctattcc gccggtgtca
     9421 gtgccggcaa tatcacggca aaattcaaag aaaccccaac cgccattcta gtcacaatca
     9481 tcggcacgct actggcaatc agtttaccgg ttacccaata tgaaaatttc ctgctgctga
     9541 taggctcggt attcgccccg atgattgccg tgcaaattgc ggatttcttt gtgttaaaac
     9601 gccaccaagc acagcctaat gttgattggg ttgctgccgg attatggctt gtcggcttcg
     9661 tgctatacca ccttctctct taccaccaag tgcatttact actcggctta accattccag
     9721 tgatgttagt gatttttctc agcaccattt ttgtgaggag gttcacaaaa tagaaagata
     9781 agcggttcaa ttggagtgaa aatttgctaa aattgagtcg cttgttttaa acaatgactg
     9841 ggtagtaaca ccacaagctc aacttgccta ccacaccatt aacggtaaag ccgatgagga
     9901 acggttaaat cttttagttg cccgtgccgg cgtaagagta gcaaaaggct ttgccttgaa
     9961 taatggttgg aatctgcaac cttatgcgga agtaaacggc attgctgaaa aaggccaata
    10021 gtacgaaagt gcaagtaaac caatatcagt ttggagttgc cgaaaacaag ggcagagtgc
    10081 aaggttcact tggcttgagt gcaggaaacg gtaatcaccg tttaggttta gaggcatcag
    10141 ttgcttcagg taagaaactg aaacaaccat ttaatgtatt agtaaattat cgttatcaat
    10201 ggtaatttaa gagggcaggt gaaaacctgc ccttaaattc aataattaat ctatcttccc
    10261 acaatttaaa cagtaaagat acatctgttt cggatcgtta aaacgtgaaa gagtctctag
    10321 ctgttgttgc ggttgcttca acagcggtaa atcaagccaa tttgccacgc taaattgtaa
    10381 ttgagctttg aaatcacggg caagcgtgcc aaataagctg tcatcttgct ccgcaaacca
    10441 ttgtaagccg aactgttcga ctttaggttg gttttccgct tcacgttttt ggttgatcaa
    10501 ttcagataac gcattatatg ccacattaaa attgcccaac tcatcaacca atccgtgttt
    10561 taacgcatca gttcccagcc acacttgccc ttgagccact ttatctacca cctcttttgg
    10621 cattttcctg ccacgagaaa ccagctctaa aaaacggtcg tagccgtttt caatcccaat
    10681 ttgcagcact tctgcctgct cttgcggtag gctttttaaa ccggcttgtt gtgctagggc
    10741 agaagtcgct atgccgtctt cgctcacacc gagattttta gcggtttttt caaaagttac
    10801 tgctaaaccg aaaataccga tagagccggt aagcgtattc gggctagcaa taattttatc
    10861 gctggtagcc gcaatccaat aaccgcccga tgctgccatt ccgcccattg aagcaaccac
    10921 cggtttgccg gcttgttgaa aggcttccac ttcttggcga atcagttcag acgccacagc
    10981 actgccgccg gggctgttaa tgcgtagaat taagccttcc acatttttgt catctcgagc
    11041 ttgttgcagt aattttacca gcgtctcgct gcccacattc atttcgtcac tttcaccgcc
    11101 agcaatcgcc ccttccacat tcactaccgc aattttcgga gcattcactt ctgcaaagcg
    11161 gtcgtttagt tcagtcatat aatctaaaaa ttcaatctgg ttataactgc cttcgctgtt
    11221 tttgccaaac tgtgcaatca gtttatcccg actttcttgg ctggacacta actgactaac
    11281 ccatttttga tttaatgcca gttgagcatc atctccttta gcttgtttat atttttcaat
    11341 gtaattggca ggcgataaat ccagtgcttg cggttcaatg ttgcggtttt gagctagtgt
    11401 tgccacagtt tgttgccaag tgccgtttaa ccagcctcgg gcattttgtt tcgcctcctc
    11461 agacatatca tcacgcataa acggctcaac cgccgattta taagtaccga cacggaaaat
    11521 atgcggttca gcctcaattt tatcgagcaa ggttttgaaa taagtgttgg aataatttaa
    11581 gccttttaaa tccaccgcac cgactttatt gagataaatt tcatcagcaa aggaagcaag
    11641 ataatattgt ttttgcgagt agtcttcgcc cactgcaatc accggcttgc ccgattgttt
    11701 gaagttgttg atttcattgc cgataaaatc caatgagctg aaatcagcac tttgtagctt
    11761 ttgcagatct aacaccaagc cggtaatttt cgggtcattt ttagcgtggc taatcgcttg
    11821 caccacatca aaagtcgaga ttttaaacgg ctcggaacta cctaattccg attgcaccaa
    11881 gcggtagaaa tcagtgaatt cttcccgatt atctgctaaa taaccatcta aatttaaacg
    11941 taacgcccct tgattgaaca ccggcttttg cgtgctgcca gaagagctga taaatgcgac
    12001 caccggcact aatagcaaaa taaacagaat aaaaaagaga ttaatgacac actctctaat
    12061 acaacggaag attcggtaaa atgcttttaa aatcgttaac atcggaaatt cctaaaaata
    12121 aactcagtcg gcacagtata aagcaatcgc taaatgaaga aaatatgcta ttcttgaacg
    12181 caaaaaatta acaaaggaac gcttatgcaa atcttggatt tattacacca tcgccgttca
    12241 aataaaaaat ttggcgataa agccccgaat gcagaacaac tggaacaaat tctaaaagcc
    12301 ggcttgcgag tgccggatca cgggcggtta aagccttatc gctttgtggt gattgaaaaa
    12361 agcggtatgc cacagtttgg tgaatgctta aagtctgcgg tagcagaatt tgatatgggc
    12421 gaggaacgct taaaaaaagc ggaaaaatta gctaatcaag ctccaatgat tatcggcgtg
    12481 gtagcaacat tagacgacag catcgccaaa gtgccaagtt gggaacaaat gattaccgca
    12541 ggctgtgcaa cttatgcgat tcagttagcg gcaaatgcac agggctttga tacggtttgg
    12601 atcaccaata aatggattaa cggctctgct cttcgctccg cttttggttg ccaagaatct
    12661 gataaaatca ttgccttaat tatgcttggc tcaccggcag agggcgagca aattgcatta
    12721 gcccctgaaa gtgaaagttt agaggggttt gtacgttatc ttaagtaagc aggttaacag
    12781 ataaaagtta gtaataaggg cgaaaaatat ttcgcccttg cttttatctg ttttcgcctt
    12841 acttcttcca gccttttaac gcatcggcaa aggcattgtt accaaaagcg ttgcgattgt
    12901 tgctttgcaa gcggtcattt ttctgcgtat ttttgcaaat ttctgctgaa tttcgaccgc
    12961 ttgttggctc ttttttatcg gttggtttgt cgtctaaacg catcgtaagg gcaatacgtt
    13021 tgcgggcagc gtccacctca agcactttga ctttcaccac atcaccagct ttcactaccg
    13081 tgtgcggatc ttcaacaaaa gagtttgaga gcattgaaat atgcaccaag ccatcttggt
    13141 gtacgccaat atcgacaaaa gccccaaagt ttgccacatt ggtcactgtg ccctccaaaa
    13201 tcataccggc tttgaggtcg gtaatttctt ccacgccgtc cataaacacg gcggttttaa
    13261 actctccacg agggtcacgc cccggttttt ctaactcttt gaaaatatcg ttcaccgttg
    13321 gtaaaccaaa ctgctcatcc acaaaatctt tggcgttgag cgagtgaatt ttgctgttgt
    13381 tacccattaa ttctgcaagt gttgaggtag ttgcctgtaa gattttttct accaccgggt
    13441 aagcttccgg gtgaacgctt gaggcatcaa gggcattttt cccaccgata attcgcataa
    13501 aaccggcaca ctgttcaaag gctttcggcc ctaaacgagg cacttttttc agctctgagc
    13561 ggctggtaaa acggccgttt tcgtcacgga atgccacgat attttgtgcc aatgttttgg
    13621 tcattcccgc cacccgagcc agcaacggag cggaggctgt attcagatcc acgcccactg
    13681 cgtttacaca atcttctact accgcatcga gtttacgggc aagttgggat tggtttacat
    13741 cgtgttgata ctgcccaaca ccaatcgctt ttggttcgat tttcaccaac tcggcaagtg
    13801 gatcttgcaa acggcgggca atcgaaaccg ctccccgtaa tgaaacgtct aaatttggaa
    13861 actcgttagc cgcaaattca gaggcagaat agaccgatgc cccagcctca ctcaccacca
    13921 ccgtttgcgg tttattctct ttgatttctt taatcaccgc tttggcaaaa cgttcggttt
    13981 cacgagaggc cgtaccgtta ccaatcgcaa tcagttccac attgtgttgt ttgattaagc
    14041 tgaaaatcgc aagctgtgcc tccgcctctc gcccagtgtg agggtaaatt gtggcggtat
    14101 ccagcaattt accggtgtta tccaccaccg ccactttcac gccggtacgc aagcccgggt
    14161 ctagccccat tgtatttctc gcaccggcag gggctgccat taataatgcc gagagattgc
    14221 gggcaaacac atcaatcgcc tcttcttcag ctttttcaca cagggttgcc attaattcgg
    14281 tttctaaatg cagagctgct ttgattttcc acgtccaagc aatcacttgc tcacgccatt
    14341 tatctgctgg ttgattatta aaaatcacgc ccaaatgctc acgaataatt tcttcacaat
    14401 ggctggattt tgtgccttct tctgcagtcg gatcggcatt taatgaaagt gataacaccc
    14461 cttcatttcg cccacggaac atcgccaaag cacggtggga aggcacgctg ttaaacggct
    14521 cgctatgagc aaaataatca cggaatttct cgccctcttc ctctttgccg tcaatcactt
    14581 tcgattccaa cgtggcgtaa gcggtcaaat attggcgaag ttttgcaagt agctcagcat
    14641 cttcgctaaa acgctccatt aaaatatatc ttgccccgtc taatgcggcc ttggtatccg
    14701 caacgccttt ttccgcatca acaaagtttg ccgctaaggt ttcaggatct tgtttcggat
    14761 cattccataa actgtccgcc aatggctcta accctgcttc aatcgcaatt tgccctctgg
    14821 tgcgacgttt tggtttatac ggcaaataga gatcttccag ctcggtcttg ctttcactgc
    14881 ttagaatttt ggcttttaat tcatcggtta atttgccttg ttcttcaatc gacttcaaaa
    14941 tggtttggcg gcgttcgtcc atttcacgca aataaattaa gcgggtttca aaatggcgaa
    15001 gttgggtgtc gtctagcccg ccggtcactt ctttacggta acgagcaata aatggaatgg
    15061 tattgccctc acctaatagc gtgatggctg caagaatttg gtgtgagcca acgttgagtt
    15121 cgccggcaat aatttggctg atttttagat ttaagtcagt cattagtttt ccttcaataa
    15181 atttgctcga ggtttatcca atttctcaag ctcggtcaat gtttcggcaa ttttttgcag
    15241 attcggctct tttttcgcac ataacaccat attttctaag ctatccgctt ggtgatagta
    15301 gggtgctttg cttttgacag cctgctcaaa ttgctgataa gcctgttgaa attgaccctg
    15361 tttacataaa aaagtgccgt aattattcag cacatcaggg cggttttgac tcaatttcag
    15421 tgccgtttgg taggcttttt cggcattttc agcatcacct gtttgttggt aataataggc
    15481 aagcaccgaa taagggaggt aatctttttc atcgtgagcc aaggctttat caatattctg
    15541 cttagctttt gggaaatcat tttgctccag ataagctaaa gccaaattaa tacgggcttt
    15601 taccgcttcc gagcggttaa attcgacttc agaggaagaa gggggaagag aagaacaagc
    15661 cgctaataaa aaaatcagaa tacaagcggt catttttgga aaaaattttg caaacataac
    15721 gttctcctac aacaaagggc ttaattttac tgttccatcc tcgctaagac tagcgatttt
    15781 ttgcgaagaa gaaaaatttt tcgttttcat ttttcgacgc agatcgcaaa aataataatg
    15841 gggtgttgtg ctagggtaaa tggtaggaat acactctcta tttaatataa gaatttcatt
    15901 gaaaaaactt ataaaactta taaaatagag gcaaaaatga aaatagtggt attccagcgt
    15961 aaagccttaa agcaattacg aaaaatccca actggtgcag aaattcgccg aaaatgtgat
    16021 gatttagctt actttcccta ctgtgccaat attaaggcat taacaaatca tcaatatgat
    16081 tatcgctttc gggtcggaaa ttaccgtatt tttttcaata ttgaaggcga aacgatgaat
    16141 attgtgtcta tcgaagaggt taaaaaacga gatgaaagaa cttactaata ttcaatattt
    16201 aaataatgaa gaaggaaaac ctgcctttgc cgtacttccg atagcaacac ttgaatggct
    16261 taagcagaaa gcaaatatta aaaccgcaat agagaccggt attccatctg ctgttgctgg
    16321 aattgcatta gataataact actcagctct gcgtgcttgg cgggaatatt taggtttaac
    16381 tcaagaagag gtggcacaac gcttggggat ttcgcaatca gcttatagcc aacacgaaaa
    16441 tgcaaaaaat ctacgaaaaa cgacccgcat aaaaattgcc tatgcgttgg gtttaaatga
    16501 agagcaatta gatttctaat aacaaaaagg caggaacctc tgccttttca ctattcagat
    16561 taatgggttt taaccgcaat accttgcccg aatttacgtt tttcaagggt gcgtttggtg
    16621 cggtcaatca catcgccagc aagctgtccg caagcggcat caatatcatc acctcgtgtt
    16681 ttacgcactg ttaccgtgaa accgtactcc atcaaggttt tttggaaacg atctacccga
    16741 gagttagagc ttttaccata aggagcttct gggaacgggt tccacggaat caagttaatt
    16801 ttacacggcg tgttttttaa tacttcagca agttggtggg cgtggtttgt gctgtcattg
    16861 acatgatcta acatcacata ttcaatcgtg accttgccgt ggttggcgtt agaaacttca
    16921 cggtatttat tcaccgaatc catcaacatt ttgatgttgt actttttatt aatcggcact
    16981 aattcatcac gcagttcatc gttcggagcg tgaagtgaaa ttgccaacgc cacatcaatt
    17041 tgctcacgca ttttgtctaa tgccggcacc acacccgaag tggaaagcgt gacacgacgt
    17101 ttagataagc cgtaagcaaa atcatctaac atgatttcca ttgccggaat cacgttattc
    17161 atattcagca atggttcacc cattcccatc atgaccacat tggtaatcgg gcgaacaccg
    17221 gtcacgccaa aattgccgat aattttagaa gcacgccaca cttgcccgat aatttccgcc
    17281 acgcttaagt tacggttaaa gccttgttga gcggttgagc agaaggtaca agccaaggca
    17341 cagcctactt gtgaagaaac acacagggtt gcacggtcat cttcaggaat atataccgtc
    17401 tcgatttgct gatcgcccac ttgcattgcc catttaatcg taccgtctgc agaacgttgt
    17461 tctaccgcca cttcaggggc tttaatttcg gcaatacgct ttaatttttc acgcaatact
    17521 ttattgatat tgctcatatt atcgaaattt tcttcgccaa aatggtagat ccatttcatt
    17581 agctggtcgg cacggaaagg tttttccccc atttctgcaa acagttctcg catttcttga
    17641 cgattaagat ttaataaatt gattttttct tttggttgtt cagcagaccc acagcctgca
    17701 ttggtaatca gttctgacat aaagttgaaa gcctcgttat tacacggatt acggcaaaag
    17761 gttagggcaa atgcccttat tttgcgattg gattttaaag cgtgcaattt tactggctct
    17821 tgagggatta ggctagtaat ttttaatcaa aagagttgaa ttgcctgagt aaacttatca
    17881 agaataacct tataaaaaca agactattta catcattccc aatgttctat agaatagctt
    17941 tcgataattc tcattctcaa taacaaaagg aggctaaaaa tgaaactatc aacaattaaa
    18001 aaagcattgg tattaacctt tggtttaagt gcagcgatgg caatggcaga gccgtttaaa
    18061 gtagttacca cttttaccgt gattcaagat attgcacaaa atgtcgccgg cgataaagcc
    18121 actgtggaat ccattactaa accgggtgct gaaattcacg attatcaacc aacaccaaaa
    18181 gacattgtaa aagctcaaaa ggcagactta gtgttatgga acggtatgaa ccttgagcgt
    18241 tggtttgaac gtttctttga aaatgtaaaa ggtaaaccgg cggttgtcgt caccgaaggc
    18301 atcaccccaa tgccgattac cgaaggtgaa tataaaaatc taccaaatcc gcacgcttgg
    18361 atgtccaccg ataacgcatt aatttacatt gaaaatattc gtgcggcttt agtaaaatat
    18421 gacccacaaa acgcagacac ctataatgcc aatgcaaaag cctatgctga aaaagtaaaa
    18481 ggtattgctg agccactccg ccaacgttta gcttttattc ctgagcagca acgttggtta
    18541 gtaaccagtg aaggggcatt tagctattta gcgaaagact acaacttaaa agaactttat
    18601 ttatgggcaa tcaatgcgga agagcaaggt tcgccaaaac aagtgaaaaa agtgattgat
    18661 ggtgtaaaag ccaacaaaat ccctgttgtg tttagcgaaa gtaccgtttc cgacaaaccg
    18721 gcaaaacaag tggcgaaaga aaccggtgca ttatacggtg gcgtaattta tgtggattca
    18781 ctttcagaga aagatggtcc ggttccaacc tatttagatt tgcttaaagt gactatcggc
    18841 accattgtcg aagggttcga acgaagcaaa aaataataag atttagcaat gacaactgtt
    18901 tcagcctcca tttccgtcga aaatttatcg gtccgttata ataacggaca tatcgctttg
    18961 caagatgtct cgtttcatct ggaaaacggc acaatttgtg cattaatcgg cgtgaatgga
    19021 ggtggaaaat ccactctgtt taaaagttta atggggctgg ttaagccatt aaccggctct
    19081 gttaagctga gtcagctccc gattgctaaa gccctcaaac aaaatttggt ttcctatgtg
    19141 ccacaaagtg aagaagtgga ctggcaattt ccagtttcgg tttacgatgt ggtaatgatg
    19201 gggcgatacg gctatatgaa ttttctacgc cgcccaagcc aaaccgataa gctcaaagta
    19261 gagcaagcaa tgcaacgcct tgatgtttta catctcaaag acaggcaaat cggcgaactt
    19321 tccggcgggc aaaaaaaacg ggtattttta gcccgtgcct tagctcaaga aagtaaaatc
    19381 atcttacttg atgagccttt caccggtgtg gatgttaaaa ccgaaaatgc gattgttgag
    19441 ctacttaaac agcttaaaaa tgaggggcat ttgattttag tttccaccca caaccttgcc
    19501 agcgtaccaa gcttttgcga tcaggttttg atggtcaatc gcacgctact ggcaaaaggt
    19561 aaaacggaag aaatctttaa taatcaaaat ttagaacggg tgttcggcgg tttattgcat
    19621 tatcaaaagt agtgtttcag caacaaatca caaacaaata gggaggcaaa tgcctcccta
    19681 tttttgtatc aatgaatctt agaatatgca aaactgagga aaaaaatggc cgcttgcacg
    19741 agaataatcg tcggccctgt tgcggcatca atatggtagc taagcaacgt gccgaatacc
    19801 gatgaaaata tcgcaacgcc taaggcaatc agtaacatac tatcaaaccg cttagtcagc
    19861 aggtaagcag tgatccccgg ggtaatcaac attgccacca ctaaaatcac gcccaccact
    19921 tgcattgcac tgataatagt taaagcaagc aaggaaagta aaccataatg caataatcta
    19981 accggtaagc ctgctacttt ggcttgatta acatcaaagc aatagagcaa gaagtctttg
    20041 cgtttcgcca ccatcaccac aaaggtaaag aggcttatga gtaaagtctg aagcaaatcg
    20101 cttttggtta cgcctaatag attaccaaat aaaatatggg ttaaatgctc accggtatta
    20161 aaggcggtaa acagcaagat cccaaaggca aacatacctg aaaacacaat ccccattacc
    20221 gtgtcttctt tcaagcgact attttctttt aaatagccca ccgataaggc acagcctaat
    20281 cctgaaaaaa acgcccctat tgagagtggt aacccggcaa gtgaggaaat caccacaccg
    20341 ggcagaacag cgtgcgaaat tgcatcgccc attaaagacc agcctttcag caccagatag
    20401 caagataaaa ccgcactcac gccagcaact aaaattgcgg taattaacgc aatttgcata
    20461 aattcaaatt gcaacggctc taaaatccaa ttaagcattt tgaacctcca cacggtgcaa
    20521 tttccgcttt tgagtaatca agccatattt aggagcaaag cagaatgcga ataagaaaat
    20581 taaggtttgt aagcacacaa ttaccgcacc ggttgcaccg tctaaaaagt agcttaaata
    20641 tgccccaatc cctgatgtca gcgtgccgat aagcattgcg atcagcacta atctagggaa
    20701 acggtcggta agtaaataag cagttgctcc cggcgtgacg accatcgcaa taaccaaaat
    20761 tgccccaacc gtttgcagag ccactactac acaggcactt aataaggtaa agaaaatcac
    20821 tttatagcgt aatggcgaaa gcccgactga caatgcgtgg tgttcgtcaa aaaagacgag
    20881 tagcaaatcc ttccaaaata ggattaaacc cagtaacgaa atcgcaataa tcagtaccac
    20941 ctgccacaag tcatcatcag caatgcctaa aatatttccc attacaatcg aattaacatc
    21001 aaccgatgtt ggattgaggg aaacaataaa cataccaagc gcaaaaaaag tcgtgaaaat
    21061 aaagccgata accgcatctt ctttaatttt cgtcaggctt tttacccaaa gaatcgataa
    21121 tgccgccaaa atcccggaaa aaaacgcacc aaaggcgtag ggcaatttta gtgcataagc
    21181 gatggcaaca cctggcacta cagcgtgtga taaggcatca ccaatcaaag accagccttt
    21241 taacatcaaa aaggcggaaa gaaaagcaca aactaacccc acgcccatac tgacaaaaat
    21301 cgctttttgc atatagttgt aggtaaaagg ctctaataaa agatcaagca taaaatccta
    21361 acaagttgtt gataatagtt cgcatatact atcatataaa ctccttttct tgtatggatt
    21421 aaacttaaat atctacttta aattgataag gatcaggata gtaataacga aaaccaagct
    21481 cttggcaaat tttgtctgct tttatcagcc ttacaagcgg ttgattttct tcagaaaaat
    21541 gcaaatccgc taacccaaaa cgttttgcca tttcactata ataatcttta cggcttgggt
    21601 gctgttcggc acataaatga aaaatacgct gcccgttcgg ctgctccaac agcaatgaaa
    21661 tggctgcaat acagtcctct aaatgcacta aattgaccgg ctgccctgct ccactcaagt
    21721 tctgttttcc cgccaaataa aaaaccgggt gccgtttttt gcccaccaag ccagctaaac
    21781 gcaaaatatc acaatgaatt ggctggctca gcaaccaatt ttccattttt actaataggt
    21841 tagcagaatc aaccactgca ttttcatcaa aaacgcccga tttcattggc aaaaccgaag
    21901 tcgagctggt gaaaaccaga tgtttcaagc ccttttctat tgcaagcgaa actaatgttt
    21961 gaataccgca accgtaattt tcagggcgaa tttggctaag gggcaaggca atcactaacg
    22021 catcagcagc aagtaattgc tcgatttcgc ttgtcactgc taacgcattt aaatctagag
    22081 gataacattc tatcgctaat tctccgaccg ttcgcttagt acctgcaaca cgccagccct
    22141 tattttgtaa tgcttcagct aaaggtagtc cgagccagcc aagccctaaa atggtaattt
    22201 ttttcatcat ttacgtttct caagattaaa attggaatga ggggaaattc aggtacaaaa
    22261 aaagcacctc gtggtgctat ctattttatt tcta
//
LOCUS       DANLUQ010000022        22100 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.022, whole genome shotgun sequence.
ACCESSION   DANLUQ010000022 DANLUQ010000000
VERSION     DANLUQ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 22100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22100
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.022"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(105..947)
                     /locus_tag="PXB27_000705"
     CDS             complement(105..947)
                     /locus_tag="PXB27_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HDL5345526.1"
                     /translation="MKVGLVLEGGGLRGIFTAGVLDVFLDEHIHIDGAIGVSAGALFG
                     VNLPSKQKGRALRYNKKYAKDKRYMGLHSLLTTGNIVNRDFAYYELPSKLDPFDQQAF
                     EDSGMDFWVTVTNVETGEPEYIKIDNVFEQMEAFRATSSMPIVSKIVEIEGKKYLDGG
                     VSDSIPVQKFIEMGYDKIIVVLTRPLEYRKKPSSMWLFKRFYKKYPKLVQRWENRYAE
                     YNQAVEQIIQLNEKQQIFVIRPSRTVKISRLETDVNKIQAMYDLGVEDAKNALAGLRA
                     YLAK"
     gene            1007..1324
                     /locus_tag="PXB27_000706"
     CDS             1007..1324
                     /locus_tag="PXB27_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HDL5345527.1"
                     /translation="MADAEFMDFLLDQLSPISGLRSKKMFGEYCLFFDNKIVAIISKD
                     YRIFVKANPQTLPLFLAENAEQFHYFAKGKVQKMHYWSIPELAIEEADELVKWIRLGL
                     QAV"
     gene            1414..2127
                     /locus_tag="PXB27_000707"
     CDS             1414..2127
                     /locus_tag="PXB27_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease"
                     /protein_id="HDL5345528.1"
                     /translation="MNQKKLVSLIVVIVLAIISQIFSFFDKKEPSKAQEKSTQSSQSH
                     QVSTSSLGNYDVQMKNDRLKQHIVDTDYYTLALSWSPAFCQKQKRNNHGKIPSHLEYQ
                     CGGSADFGWVIHGLWPQSGKARSVDEHPRYCQGDLPMVAEQTIKQYLPESPGATLLQG
                     EWEKHGACAFNQADDYFAKQKELFNQLKLPDTEMSKLALFNWMRANNPHLQNVYLGGS
                     KDELYICYDKAFQPMDCPR"
     gene            2127..2621
                     /locus_tag="PXB27_000708"
                     /pseudo
     CDS             2127..2621
                     /locus_tag="PXB27_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
     gene            2726..4006
                     /locus_tag="PXB27_000709"
     CDS             2726..4006
                     /locus_tag="PXB27_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL5345529.1"
                     /translation="METIVIVGGGAGGLELATYLGNKLGRKQKAKVVLIDRNATHLWK
                     PLLHEVATGTIDEGTDALSYRAHAKNHSFEFQQGTLVAVDREKKTVSLAPIYNDQNQL
                     LVKERHISYDKLVIAIGSKSNDFGTKGVAENCIFLDGSEQAKDFQKRMMELFLRFSHS
                     ENKDVKIAIVGGGATGIELSAELYHVVKNLNSYGFGKLNRASLKVTLIEAGPRLIPAL
                     PEKVSVSAFHELKKAGVSVRLGTMITEARPDGLMTRLGEKIEADIMVWAAGVKAPEIT
                     REFGFETNRLNQIEIKDTLQTTVDESVFVIGDCASLIQDGKPIPPRAQAAHQMATRCG
                     KNIVALIEGKEMKPFRFNDKGSLLSFSKFGTVGNLMGNLVSGDMFIEGKIARLAYLSL
                     YRMHQVALHGCVKTGLIVLVGQINRFLRPTMKLH"
     gene            complement(4093..5130)
                     /locus_tag="PXB27_000710"
     CDS             complement(4093..5130)
                     /locus_tag="PXB27_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5345530.1"
                     /translation="MNVLKLISLKSILIASGIFLSAQAAQAEGRLTVYCSVQNNVCEE
                     MTKQFSHKYNVETKFIHGGTGTIFGKVKAEASNPQADIWYGGTIEPHFQAGELGLLEA
                     YRSPKQAEILPQFKSLVESEQGKFTSIVYMLVLGLGVNTEKLAKLGIEAPKKWEDLSD
                     PRLKGEVQLPDPRSSGTTYTFMTTLSTLWGEEKTFDYLKKLNNNVSQYVKSTLVTSNL
                     ARGESAVTVGFVHSYATEKEKGAPIEAIIPEGKVGYALGGVSIIKNARNLDNAKLFMD
                     WVLSKEAQEIPWKKHGVYQTPTNINAEVAPQSTKAEALDFVEIDYQKAGSSEEGKRLI
                     DKWLAEIKLAN"
     gene            5352..6046
                     /locus_tag="PXB27_000711"
                     /pseudo
     CDS             5352..6046
                     /locus_tag="PXB27_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249137.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
     gene            6057..6359
                     /locus_tag="PXB27_000712"
     CDS             6057..6359
                     /locus_tag="PXB27_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDL5345531.1"
                     /translation="MFAALVLGSQICRFLPEFLPKKVLSSPILQKLNKMLPLVIMLLL
                     LLTSLNFPKNGEGFNLILAQILALIGVLGSYVWLKNTFLSVALGIFGVNVFLSLLG"
     gene            complement(6480..7781)
                     /locus_tag="PXB27_000713"
     CDS             complement(6480..7781)
                     /locus_tag="PXB27_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL5345532.1"
                     /translation="MSLFKFEERGSNARQEIIAGLTTFLAMVYSVIVVPGMLSQAGFP
                     AESVFIATCLVSGLGSILIGLWANAPMAIGCAISLTAFTAFSLVLGQQVSIPVALGAI
                     FLMGVVFTLISATGIRTWILRNLPSSIAHGAGIGIGLFLLIIAATNVKLVVNSGMDIP
                     VKLGDFTSFPVLVSLIGLAAIIGLEKLKVKGSILWVIIAITIVGLIFDPNVKYTGFFK
                     MPSFGENSQFLNLDIMGALNTAILPVVFALVMTAIFDATGTIRAVAGQAGLLDKDGQI
                     INGDKALTSDSFGSILSGLFGTAPAAVYIESAAGTAVGGKTGLTAVVVGIGFLFMLFF
                     QPLAFLVPSYATAPALMYVGLLMLSNVSKLDFSDFVGAMSGLVCAVFIVLTANIVTGI
                     MLGFATLVIGRLISGEANKLNLGTVIIAIILVAFYAFDLAI"
     gene            complement(7914..8888)
                     /gene="secF"
                     /locus_tag="PXB27_000714"
     CDS             complement(7914..8888)
                     /gene="secF"
                     /locus_tag="PXB27_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HDL5345533.1"
                     /translation="MAIENVQQDVAEIKLPYKLIPFMKYRYVGFIFSLIVTALCIFTI
                     ATKGFNWGLDFTGGTVIETQFSQPADLAKLRTELENSGYGNALVQTTGSQKDLMIRLA
                     ASAGDMSVGNKVMEMIHQKLDPNATIKSIEFVGPNVGEELTQGAIYATLATLAMLLIY
                     VGTRFEWRLAVGGIVALFHDVLVTIGIFSFLQIEIDLTFVAAILSVVGYSLNDSIVVF
                     DRVRENFSKVRRSSSIEIIDISLSQTLSRTLMTSVTTLFVVLALFYLGGPTLHSFSLA
                     LLIGIGFGTYSSIYIAIGVALQLGLKREHMVQPKVEKEGADQDAFIDY"
     gene            complement(8898..10739)
                     /gene="secD"
                     /locus_tag="PXB27_000715"
     CDS             complement(8898..10739)
                     /gene="secD"
                     /locus_tag="PXB27_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HDL5345534.1"
                     /translation="MLNRFPLWKNLMVIFVIAIGALYALPNLYGDDPSVQISGTRGQQ
                     ATPETLSQVQSTLSAMNIQPKSAVLENGSILVRLESSEQQLPAKEKISEALGNNYSVA
                     LNLAPATPQWLSDIGGSPMKRGLDLLGGVRFLMEVDMNTALAKQQDTLQDSLRSDFRK
                     EKLQYQAVKKGENFATEVEFADNDTAEKAVRLVRLSHPTLDILVNDNKVTLTPSAQAL
                     SESRTMAIEQNLSILRKRVEELGVSEPTIQRQGADRIVVELPGVQDTARAKELLGATA
                     TLEFRLVNTEANVASAAKGIVPADSGLQNTRDGEPVVLRRKPSLGGEHIIDATSGTDE
                     RGLPQVSIRLDTEGGEMMGEITKMAIQKPMATLYTEFKDSGRKDANGKVILEKHQEVI
                     NVATIQTRLGNQFQITGINSPAEAQNLAVLLRSGALIAPIVIVEERTIGASLGADNVK
                     QGMEASILGLGLTILFCLAFYKVFGIFASLALIVNMILTIGLMSLIGATLTMPGIAGI
                     VLAVGMSVDANVLIYERIKEEIRNGRPIQQAIHEGYNGAFSSIFDSNLTTILTSLVLY
                     GVGTGPVKGFAVTLALGVIISMFTAITGTRMLVNWVYGGKRVKKLWI"
     gene            complement(10779..11072)
                     /gene="yajC"
                     /locus_tag="PXB27_000716"
     CDS             complement(10779..11072)
                     /gene="yajC"
                     /locus_tag="PXB27_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDL5345535.1"
                     /translation="MQQGGGMEMIFILIIFGAIFYFMIYRPQAKRQKQQRDLLASLTK
                     GDEVLTSGGLIGKITKVTADNDNIVIALNDTTEVTIKRDFVVAVLPKGSLKSL"
     gene            11273..11830
                     /locus_tag="PXB27_000717"
     CDS             11273..11830
                     /locus_tag="PXB27_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HDL5345536.1"
                     /translation="MKKLFAQAVIFGAFFASSAFAHNVQLNQALPAVSVAKDGELAVA
                     GGKVSYKNWHSSSLAGKVRVIHHFAGRSSVKEKNEDLMTAIKNAGFDRSKYQTVTIVN
                     ADDAIVGTGVFVKNSVENGKLDNPHSQVVLDQQGSVKKAWGLKEKESFIAVLDKSGKV
                     QFTSEGKLSAKQIQEVIGLVKTLTQ"
     gene            complement(11871..12839)
                     /locus_tag="PXB27_000718"
     CDS             complement(11871..12839)
                     /locus_tag="PXB27_000718"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HDL5345537.1"
                     /translation="MKLFTLENAFLRITLSDVGASWLSCIVKMPNEEREVLVTTSAKN
                     WQKQTAFFGATIGRYANRIANAEYRLNGKTYQLAKNNGEHNLHGGELGADKIVWQLES
                     QSEQAVKFSYFFADGEEGFGGEVKAFVEYRLTENELQISFDAVANQDTPLCLTNHAYF
                     NLQGSETILDHELIINADGYLPVDASGIPNAPLKKVESTSFDFRNAKKIGQDLLADDD
                     QKAVKGYDHSFHLAKNSENPTACLAVEDLKMELRTSMPALQLYTGNWLKGQPNLTKGE
                     YEDYAGVALEPEFFPNSLNQPELLQFGGITKAGEEYHHTISYRFII"
     gene            complement(12965..14128)
                     /gene="galK"
                     /locus_tag="PXB27_000719"
     CDS             complement(12965..14128)
                     /gene="galK"
                     /locus_tag="PXB27_000719"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDL5345538.1"
                     /translation="MKPQEIALQQFEQHFGYKAERTVFAPGRVNIIGEHTDYNDGFVM
                     PCAINYGTAVSFAKRSDNKWRVFAIDLNQQDEFDLSQAIEPSEQKWANYVRGVVKFIQ
                     AKHPEFKQGADLAITSDVPMSSGLSSSAALEVSIGKTAQLLGDLPLSLPQIALIGQEA
                     ENKFVGCNCGNMDQLTSALGQQDHLVMIDCRSLEITPTPVPQGYSIAIINSNVKHDLV
                     TGEYNSRREQCEFAAHFFGKAKLRDVTAEQFIERASELSEENELAYKRAKHIISENQR
                     VLEAVEALKANDMVKLGELMAQSHDSMRDDFEITCPEVDCLAEIAQQAIGKNGGARMT
                     GGGFGGCVVCLVPNDKVEDLRQAVANNYEKQTGIKETFHVCTASDGVRECPAR"
     gene            complement(14138..15184)
                     /gene="galT"
                     /locus_tag="PXB27_000720"
     CDS             complement(14138..15184)
                     /gene="galT"
                     /locus_tag="PXB27_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HDL5345539.1"
                     /translation="MSEFVLNDHPHRRFNPLKNQWILVSPHRAKRPWQGQQEEIVQDE
                     KPSYDPSCYLCPGNKRITGEQNPNYTKPFVFKNDFSALLPDTPAPIPNDDPLFQISHT
                     QGESRVICFSPDHSKTLPQLSIEEITDVVTVWQEQANELKEHYQWVQIFENKGSMMGC
                     SNPHPHGQIWASNFLPNEIAIEDECQAKYFAQYGSPLLLDYAQRELDKQERIVAETED
                     WIAVVPYWAAWPFETLLLPKAKQFKSITDLNEAEKADLALALKKLTTRYDNLFNISFP
                     YSMGFHFAPFNGKENAHWQLHAHFYPPLLRSATVRKFMVGYEMMAESQRDLTPEQAAE
                     RLNAVSDTKHYKDK"
     gene            15397..16248
                     /locus_tag="PXB27_000721"
     CDS             15397..16248
                     /locus_tag="PXB27_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF817 domain-containing protein"
                     /protein_id="HDL5345540.1"
                     /translation="MERLNKFDAWLMNHKPNARLSGASRLLTEFWFFGLKEARACLFA
                     GLFFIAMFSVPKTGLWGLPRYDILLIFAIVVQIAMVKLGLETWDELKAISLFHLVGFV
                     LEVFKTSSNIQSWAYPDFAYTKLFNVPLFTGFMYAAVGSYIIQAWRLFDLKIKSHLPY
                     WLATLTALMIYLNFFTHHYIGDYRWYIAAFALGLYARTFVFFTPYDITRKMPLLLSFF
                     LIGFFIWLAENIGTFAGIWRYPNQFGAWSMVHMSKWSAWALLVMMTFTIIVHLKHIKQ
                     TVRVSSD"
     gene            complement(16334..17419)
                     /locus_tag="PXB27_000722"
     CDS             complement(16334..17419)
                     /locus_tag="PXB27_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL5345541.1"
                     /translation="MEYRYYITTALVIAVLQLFLFNFAKTVSWLFNLSSKNRKTVGIL
                     TFLVANGLVILHLTRTFALFRIMAFILVLLLFAFFISFFTACIYKIFNGNTTVNRWLK
                     IAYPFAFFGLLGLGYYNAYTPTVVHYSVKLDKPLEKPLRIGMASDLHLGKLFGTKQLD
                     QLAEIFNQQKVDLILLPGDIMDDNTEVYVADKMQPHLAKLKAPLGVYATLGNHDFFGA
                     QETIAKEITDAGITVLWDQTAEINGKFTLIGRNDDLVKNRPSAEQLLQNVNTKLPVIL
                     LDHRPTEIEQHAKLPIDIQVSGHAHKGQVFPASIITKLIYRLHYGYEKIGFGHYFVTS
                     GYGFWGVPMRLGSQSEVMIIDVEGENP"
     gene            complement(17441..18421)
                     /locus_tag="PXB27_000723"
     CDS             complement(17441..18421)
                     /locus_tag="PXB27_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDL5345542.1"
                     /translation="MLFDSIQFSIGVTLPTILLMLFGIFPRRRKFIDDTFCNMASKVV
                     FNFTLPPMIFLNMLKSPLDFSSQINLISTGVGGSILIYLWAEWWAARHIENRRYRCIF
                     TQGTFRTNAAILGIALILNAYGDKALAAASVYISCLVITFNVLAVITLTSSLSEKKVN
                     FGRLILSILRNPLILAVIAGLTFNYFELSHQIPTPLESAGKLMASLTLPLALICTGAS
                     INFKQLKQFNQGVESTINKIVLFSASIRLIFAPIFLLLLGKFVFQLPPMELGIVFVAA
                     SAPVASATYAMTRNYGGDGEAAANLIGITTLGSMFSASIGLFLLRQIGWV"
     gene            complement(18524..19622)
                     /gene="prfB"
                     /locus_tag="PXB27_000724"
     CDS             complement(join(18524..19546,19548..19622))
                     /gene="prfB"
                     /locus_tag="PXB27_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014550889.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDL5345543.1"
                     /translation="MFELNPIKNQLTDLSERTAVIRGYLDFEHKVERLEEVNAELEQP
                     DVWNKPEKAQALGKERAMLEQIVNTIKALEQGIEDVEGLIELAVEAEDQDTFNEAQVE
                     ADELEKKLADLEFKRMFSGPHDSADCYLDLQAGSGGTEAQDWTEMLLRMYLRWAESKG
                     FKAELMEASDGDVAGIKSATVKISGDYAYGWFRTETGIHRLVRKSPFDANNGRHTSFS
                     AAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNKTESAVRITHIPSGIVVQCQN
                     GRSQHQNKDQAMKQLKAKLYELEMSKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DHRTGVEHRTTQQVLDGDLDKFVEASLKAGL"
     gene            19878..20786
                     /locus_tag="PXB27_000725"
     CDS             19878..20786
                     /locus_tag="PXB27_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDL5345544.1"
                     /translation="MAKRPSDIDATLLQLEILRRIPKLPAKIDAKSLHQQLLDAGFER
                     DIRTVQRSLKMLCEHFDIECDERSKPFGYSWKIRSEGLALPILNEQQSLLLKLAEQQL
                     KYLLPANLMASMKPFFEQADRMVSGAKDKPEYQWLNKVCSTPTSLPLIPAKIKEEIFN
                     SVSQALFQNKLLKIEYQNQHGKKHQATIMPLAISQQGASIYLVCRYEGFDDNRLLALH
                     RIRKAEISTFSFERPKDFNLQAYQDAGHLGFGNGEKASLTFSISRDAGFHLTETPLAK
                     DQKILEESETHYRIQAIVADNDMLTW"
     gene            21087..21728
                     /locus_tag="PXB27_000726"
     CDS             21087..21728
                     /locus_tag="PXB27_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HDL5345545.1"
                     /translation="MPFQLTFCQQAGNQHQKNQDALFNGKAVYQWQLKNAESYLLDND
                     KVIFGVADGVSSHPRNHLASHYLMALLSQCQSLDSQWLRQAHGDFCEKLAKDYFGASC
                     TFVACELHHNGRGRMVNVGDSRAYKITANGEWQQLSFDHTILAEMKQKGLVKEGSNYA
                     SMYNGLTDCIVADFDADDFRIHIAEFQLEKGESILLCSDGLHEQLAPEAIQAI"
     gene            21895..>22100
                     /locus_tag="PXB27_000727"
     CDS             21895..>22100
                     /locus_tag="PXB27_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HDL5345546.1"
                     /translation="MKNDFHYAAELIRNADGILITAGAGMSVDSGLPDFRSVGGLWNS
                     YPPLKKFNLQFMQIATPLAYQERP"
BASE COUNT         6338 a         4553 c         4693 g         6516 t
ORIGIN      
        1 gtttggacag gcacaggacc ggtccctata aaatttgttt ggaagtgttt ccgtctagac
       61 gtctataata tattttttga acgttttagg caaatagaat ttgtttattt ggctaaatat
      121 gcccttaaac cagccaacgc atttttggca tcttctaccc ctaaatcgta cattgcttgt
      181 attttattta catccgtttc aaggcgactg attttgactg ttctgcttgg acgaatcaca
      241 aaaatctgtt gtttttcatt gagttgaata atctgctcaa cggcttgatt atattccgca
      301 tagcggtttt cccagcgttg cactaatttc ggatattttt tataaaaacg tttaaacagc
      361 cacatcgagc ttggtttctt acggtattca agtgggcgag taagcaccac gataattttg
      421 tcatatccca tttcaataaa tttctgcacc ggaatactgt ctgacacacc accgtctaaa
      481 tattttttac cctcaatttc gactattttg gatacaatcg gcattgagga agtagctcga
      541 aaggcttcca tttgctcgaa cacattgtca attttgatgt attcgggctc accggtttcg
      601 acattggtta cagtaaccca aaagtccata ccggaatcct caaaggcttg ttggtcaaac
      661 ggatccagtt tggaagggag ttcatagtaa gcaaaatccc gattaacgat attgccggtg
      721 gtaagcaagc tgtgtaaccc catataccgc ttatctttag cgtatttttt gttgtatcgc
      781 aacgccctac ctttttgttt tgaaggcaaa ttcacgccaa ataacgcacc ggctgaaaca
      841 ccgattgctc catctatatg aatatgttca tctaaaaata catctaacac gccggcagta
      901 aaaatacctc gcaaaccacc gccttccagc actaatccaa ctttcatatc attctccctg
      961 taatttgtgt attataaccc gaattatctt gctaggagaa atcacaatgg ctgatgcaga
     1021 atttatggat ttcctgttag atcaactaag cccgatttcc ggtttgcgtt cgaaaaaaat
     1081 gtttggtgaa tattgcttgt tttttgataa taaaattgtc gctattatca gcaaagatta
     1141 tcgcattttt gtcaaagcta atccgcaaac cttgccgtta ttcttagctg aaaatgccga
     1201 acaatttcac tattttgcta aaggaaaagt gcaaaaaatg cactactgga gcattccgga
     1261 attagcgatt gaagaagccg atgaactggt caaatggatt cggctaggct tacaagcggt
     1321 gtaatttagc gaaaattttg taaaaaaacg ctaaaatatg cccgcttgtt agggggggga
     1381 cctaatattt ctatacttta agattttata tttatgaacc aaaaaaaatt agtttctttg
     1441 attgtcgtta ttgtgttggc aatcatttct caaattttta gcttttttga taaaaaagag
     1501 ccttcaaaag cacaagaaaa atcgacccag agtagtcagt ctcatcaagt ttcaacttca
     1561 tcactaggga actatgatgt gcagatgaaa aacgaccgtt tgaaacagca tattgtagat
     1621 accgattatt acaccttagc gttgtcgtgg tcgccggcgt tttgccaaaa acaaaagcga
     1681 aataatcacg gtaaaattcc gtctcatttg gaatatcaat gtggtggctc agcggatttt
     1741 ggttgggtta ttcacggctt atggccgcaa agtggcaagg caagaagtgt tgatgaacac
     1801 cctcgttatt gccaaggcga tttgccgatg gtggcagagc agacgattaa gcaatattta
     1861 cctgaatcgc cgggggcaac tttgttgcaa ggcgaatggg aaaaacacgg tgcttgtgcc
     1921 tttaaccaag cggatgatta cttcgccaaa caaaaagagc tatttaatca gctcaaatta
     1981 ccggataccg aaatgtctaa attagcactg tttaactgga tgcgagccaa taaccctcat
     2041 ttgcaaaatg tctatttagg gggtagcaaa gatgagctgt atatttgcta tgataaagca
     2101 tttcaaccaa tggattgccc acgctaatgg cagatcttcg ccaactgaaa atgcaggttc
     2161 agcgccaatt aaagcgtgat cttgaacgtg caaatcacta ttttaatacc acttttacgc
     2221 caccaagtat tagctatgcg gtgcgtggcg taaaagccgg tgtggcttat cttgaacgca
     2281 acgaagtacg gtttaatcct gtgttattgt aagaaaatga acaggcattt atccgccaag
     2341 ttgtgccgca cgaactggct catatcttgg tttatcaaca ttttgggcgg gtacaatctc
     2401 atggtaaaga gtggaaaatg atgatggaaa ccgtactggg tgtgccggcg gaaatttacc
     2461 attgttttga tacccaaagt gtgcaacagc aatttagtta ccaatgtgcc tgccagactc
     2521 accagctttc gattcgccga cacaatgccg tgatgcgaga taaaaaaagc tatatttgcc
     2581 gtttatgtaa aactcaactc cattttgtag aaaaaagtta atgtggagaa aagttaagag
     2641 aatgttacga tttagctaga cattcctgtt taaacgccta caatttgccg tagtttttat
     2701 ttttaatttt atttccggag tatttatgga aacaattgta attgtaggtg gcggtgcagg
     2761 cggtttggag cttgcaacct atctaggcaa caaattaggg cgtaaacaaa aggcaaaagt
     2821 ggtgctgatt gaccgcaatg caacgcattt atggaaacca ctgttgcacg aggtggcaac
     2881 cggtactatt gatgagggaa ctgatgcgtt aagctatcgg gctcacgcta aaaatcatag
     2941 ttttgaattt cagcaaggta cgctggtggc ggtagataga gagaagaaaa cggtttcact
     3001 cgccccgatt tataatgatc aaaaccaact tctcgttaaa gaacgccata tcagttatga
     3061 caaattagtg atcgcgatcg gcagtaaatc caatgatttt ggcaccaaag gggttgcaga
     3121 aaattgcatt ttcttagatg gctcagagca agcaaaagat ttccaaaaac gaatgatgga
     3181 gctgttttta cgtttttcac atagtgaaaa taaagatgta aaaattgcga ttgtgggggg
     3241 gggagcaaca ggtattgagc tttcagcgga gctttaccac gtggtgaaaa atttaaattc
     3301 ttacggcttt ggtaaattaa accgtgccag cttgaaagtc actttaattg aggcaggccc
     3361 tcgtttgatt ccggcattac cggagaaagt gtcggtttct gctttccacg aattgaaaaa
     3421 agcgggtgtt tcggtgcgtt taggtacgat gattaccgaa gctcgtccag atgggctaat
     3481 gacccgttta ggtgagaaaa ttgaggcgga tattatggtt tgggcggctg gcgtgaaagc
     3541 ccctgaaatt acccgtgaat ttggttttga aactaaccgc ttgaaccaaa ttgagattaa
     3601 agacacgcta caaaccaccg ttgatgagtc agtgtttgtc attggcgatt gtgcatcatt
     3661 aattcaagat ggcaagccaa ttccacccag agcacaagca gcccaccaaa tggcaacccg
     3721 atgtggtaaa aatattgtgg cattgataga aggaaaagag atgaagccgt ttcgatttaa
     3781 cgataaaggc tcactgcttt ccttctcaaa attcggcacg gtgggtaatt taatggggaa
     3841 tttagtgagc ggtgatatgt ttattgaagg taaaattgcc cgcttggctt acctctcgct
     3901 ttatcgaatg caccaagtgg cattacacgg ctgtgtcaaa accggcttga ttgtgttggt
     3961 agggcaaatt aaccgtttct taagaccaac aatgaaacta cattaaacaa aaatccgcat
     4021 caaatgatgc ggatttttta tggcaaaata acaagcggtt attttcttgc ctatttttgc
     4081 aaatactcac gcctagtttg ctaatttaat ttccgccaac catttatcaa ttaagcgttt
     4141 gccctcttcg ctggaacctg ctttttggta atcaatttcc acaaagtcta acgcttcggc
     4201 tttggttgat tgtggagcaa cttcggcgtt gatgtttgtt ggggtttggt aaacaccgtg
     4261 ttttttccaa ggaatttcct gtgcttcttt tgaaagcacc caatccataa agagtttggc
     4321 gttatctaaa ttacgggcat ttttgataat gctgacaccg cctaacgcat agcccacttt
     4381 accttccgga ataatggctt caataggcgc acctttttct ttttcagtgg cataactatg
     4441 tacaaagcct accgtaaccg cactttcacc acgagcaaga ttgctggtga ctagggtgct
     4501 tttcacatat tgcgaaacgt tattgttgag ttttttcaga taatcaaagg ttttctcttc
     4561 gccccataag gttgaaagag tggtcataaa cgtataagtc gtgccggagc tacgaggatc
     4621 aggcagttgt acttcacctt ttaagcgagg atctgataaa tcttcccatt ttttcggtgc
     4681 ctcaatgcct agtttggcga gtttttcggt attcacgcct aaccctaata caagcatata
     4741 aacgatagag gtaaatttac cttgttcact ttcaactaag gatttaaatt gtggcagaat
     4801 ttcagcttgt tttggtgaac ggtaggcttc cagtaagcct aattcgcctg cttgaaagtg
     4861 aggttcaatc gtgccgccat accaaatatc tgcctgaggg ttcgatgctt ccgcttttac
     4921 ttttccgaaa attgtaccgg ttccgccgtg aatgaattta gtttcgacat tatatttgtg
     4981 tgaaaattgc ttggtcattt cttcacaaac attattttgt accgagcaat aaacagttaa
     5041 acgaccttct gcttgtgctg cttgagctga taagaaaatg ccagaggcga ttaagatcga
     5101 ttttaatgag attaatttga gtacgttcat cactttcctg aaaacataaa ataaaaggta
     5161 gtgaagtcta gcatttcaaa gcgctggttg aaaatagtta attgcaatga agtagggcaa
     5221 ttgtgcctat tactttaata tttaccaact atttgcaagt tttagcaaaa aataaccgct
     5281 tgtagtagct cttgctattt tgtcaaaatt cagaataata aatgccttta gtctaatgtt
     5341 aaattttagt tatggaaaat agttggaaat atgcttttaa attaatcacg ccgattttta
     5401 tgggctattt tgcggcaggg gttgcttacg gaatgcttgc caccaatgcc ggaatgcctg
     5461 cgtggtttac gattgtaatg agttttaccg tggtatcggg gacggcacaa tatgcagcga
     5521 ttccattttt tgtctccggt gttggggcgg tgtcggtctt tctcagtacg cttttaatga
     5581 gtctacgttt tagtttttac accttaaata tgatggagca taagccgata tccaaagcga
     5641 aaagtctggt cgctattgca ggtttaactg atgaaggctt tgcggtgtta tcttcattac
     5701 cgaaagagca aaagcaagca ttattcttta aaatcgctgt gctatgtgtg gcttattgga
     5761 cattttccac gattgtcggt gtgttgttag gcgattcagt ggcgaactat attccgcatt
     5821 tggattttgc tctgccttgt ttgcaatttt agcctatgag caatacaaaa accaaaaaca
     5881 atggaaaccg attgtgattg cgttaatcgg atttttactt gctcgtcaga ttaccgaaac
     5941 cagcgtgtta ttggtggcaa ttttagtagc gattgtcatt gtggctttat tacctcaatc
     6001 ttcttcaatc aaactagaga aaggagtaga aaatgagcac aaatgatact ttgataatgt
     6061 ttgcagcatt agtgctcggc tcgcaaattt gtcgtttttt gccggaattt ttaccgaaaa
     6121 aggtgttatc ttcgcccatt ttgcaaaaat taaacaaaat gttaccgctt gtgattatgc
     6181 ttttattgct tttaacgagc ctaaactttc ctaaaaacgg ggaaggcttt aacttaatat
     6241 tggcacaaat tctggcttta ataggcgtat taggtagcta tgtttggctt aaaaatacct
     6301 ttttaagcgt agcgttgggg attttcggtg tgaatgtatt tttatccctg ttaggataaa
     6361 taccaaaggt cattttatag gagcgaaaca ccttcgctca atcattaaca gatgtggggc
     6421 gaaaaatttt tcgcccctta tttatcacaa gcggtctttt tctttcaatt ttttgcaaat
     6481 taaatcgcta aatcaaacgc atagaaggca actaaaataa tcgcaataat tactgtacct
     6541 aggtttaatt tattggcttc gcctgaaatt aagcgaccaa tcacaagggt ggcgaaacct
     6601 agcataatgc ctgtcacaat gttggcggtt aatacgatga acaccgcaca gactaaacct
     6661 gacattgcac ctacaaaatc gctgaaatct aatttagaga cattacttag cattaataag
     6721 cccacataca ttaatgccgg tgcggtggca tagcttggta ctaagaatgc aagaggttgg
     6781 aagaacagca taaataagaa accgatacca accactacag cggttaaacc tgttttaccg
     6841 ccaacggctg tacctgctgc cgattcaata tacactgccg ctggtgctgt accaaataag
     6901 cccgataaaa ttgatccgaa tgagtcagaa gttagggctt tatcgccatt gataatttgt
     6961 ccgtctttat ctaataaacc ggcttgccct gcaaccgcac ggatagtacc tgttgcatca
     7021 aaaatagcgg tcattactaa cgcaaacact accggtaaaa tggcggtatt taatgctccc
     7081 ataatatcaa gatttaggaa ttgcgaattt tcaccaaaac tcggcatttt gaaaaagcct
     7141 gtgtatttta cgttagggtc aaaaattaaa cctacgatgg taatagcaat aatgacccat
     7201 aaaatactgc cttttacttt cagcttttct aacccgataa tggcggctag accgattaat
     7261 gacactaaaa ccggaaatga agtgaagtcc cctaatttaa ccggaatatc cataccgcta
     7321 ttgaccacta atttaacgtt agtggcggca ataatcagta agaataaacc aataccaatt
     7381 ccagcaccgt gggcaatgct gcttggcaaa ttacgtaaaa tccaagtacg aataccggta
     7441 gcagaaatca aggtaaatac tacgcccatt aagaaaatcg ccccgagtgc aacaggaata
     7501 gaaacttgtt gccctaatac taagctaaag gcagtaaaag cagtaagtga aatcgcacaa
     7561 ccgattgcca ttggggcatt tgcccataaa ccgattaaga tagagccgag acctgaaact
     7621 aaacaggtgg caataaatac cgattcggca ggaaagcctg cttggcttag cattcccggc
     7681 acaacgatga cggaataaac catggctaaa aaggtagtta aaccggcgat gatttcttga
     7741 cgagcgtttg aaccacgctc ctcaaattta aataacgaca taagaacctc aaaagaagaa
     7801 aatgaaaggg gaaattttag gatgcggaat tttacagaaa tttaaaataa ttagcaaacg
     7861 tttgcgtaaa aagtgaaaga aaagcaggaa taaatcctgc ttttgtttga tggttagtaa
     7921 tcgatgaaag cgtcttgatc tgccccttcc ttttcaactt taggctgaac catatgttca
     7981 cgttttagcc caagttgtag tgccacacca attgcaatgt agatggatga ataagtaccg
     8041 aaaccgatac cgatcaataa tgcaagtgag aaactatgaa gcgttggacc acctagatag
     8101 aataaggcta gtaccacaaa gagggtggtt actgatgtca ttaaagtacg cgaaagtgtt
     8161 tggctcaagg aaatatcgat aatttcaatt gagctggagc gacgaacttt agaaaagttt
     8221 tcacgtacac ggtcaaacac cacaattgag tcatttaacg agtaaccgac aacagataaa
     8281 atcgctgcta caaaggtcaa gtcaatttca atctgtaaga aagagaagat tccgatcgtc
     8341 actaatacat cgtgaaataa agccacaata ccgcctaccg ctaaacgcca ctcaaaacgt
     8401 gttcccacgt aaataagtag catagcaagt gttgccaaag tggcataaat agccccttgg
     8461 gttagctctt cccccacgtt tgggccaacg aattcaatgc tcttgatagt cgcattcgga
     8521 tctaattttt gatggatcat ctccatcact ttattcccaa cagacatatc accggcactg
     8581 gcggcaagtc ggatcattaa atctttttga ctgcctgtgg tttgcactaa ggcattgccg
     8641 tagccgctat tttcaagctc cgtacgcagt tttgctaaat cagcaggttg tgaaaattga
     8701 gtttcgatta ctgtcccgcc ggtaaaatct aacccccagt taaagccttt tgtcgcaata
     8761 gtaaaaatgc ataaagcagt tacaatcagc gaaaaaatga atccgacata gcggtatttc
     8821 ataaagggga ttaatttata aggcagttta atttctgcta catcttgttg tacattttca
     8881 atagccattt ttccacctta aatccaaagt ttcttcacgc gtttgccacc gtaaacccag
     8941 tttacaagca ttcgagtacc tgtaattgcg gtaaacatag aaataattac ccctaatgca
     9001 agcgttaccg caaagccttt taccggacct gtacctacac cataaagcac aagagaagtc
     9061 aaaatagtgg tgaggttgga gtcaaaaata cttgagaatg cgccattata accttcgtgg
     9121 attgcttgtt gaattgggcg accgttacga atctcttctt tgatccgctc gtagattagt
     9181 acgttggcat caactgacat acccacagca agtacgatac cggcaattcc cggcatagta
     9241 agggttgcac cgattaatga cattaaacca atcgttaaga tcatattcac aatcaaggca
     9301 agagaggcaa atataccaaa tactttataa aaggctagac agaacaggat cgttaagcct
     9361 aagcctaaaa tactagcttc cataccttgt ttaacattat ctgccccaag agaagcaccg
     9421 atagtacgct cttccacaat cacgattgga gcaatcaacg cacctgaacg taataatacc
     9481 gcaaggtttt gagcttccgc tggtgaatta atccctgtaa tctggaattg attacctaaa
     9541 cgggtttgaa tggttgcaac gttaattacc tcttggtgtt tttctaaaat gactttaccg
     9601 ttagcatctt tacgacctga atctttaaat tcggtataaa gtgttgccat cggtttttgg
     9661 attgccattt tagtaatttc acccatcatc tcgccgcctt cggtatcgag tcggatactc
     9721 acttgcggca agccacgctc atcagtgcct gaggttgcat caataatatg ttcaccccct
     9781 aacgaaggtt tacggcgtaa tacaactggc tcgccatcac gggtgttttg cagtcctgaa
     9841 tcagccggca caatgccttt cgccgcagag gccacattgg cttcggtatt gactaaccgg
     9901 aactcaaggg ttgcagttgc ccctaaaagc tctttggcac gagcggtatc ttgcacaccc
     9961 ggtaattcaa ctacgatacg atctgcacct tgacgctgaa tggtcggctc agaaacgccc
    10021 aattcctcaa cacgtttacg caaaatagaa aggttttgtt cgattgccat cgtgcgagat
    10081 tcagaaagag cttgtgctga tggcgttaag gtgactttat tatcatttac caaaatatca
    10141 agcgttgggt gagagagacg cactaaacgc actgcttttt cggcggtatc gttatcggca
    10201 aattcaacct cagttgcaaa attttcgcct tttttgaccg cttgatattg caatttttct
    10261 ttacggaaat cactacgcag gctgtcttgc aaagtgtctt gttgttttgc aagcgccgtg
    10321 ttcatatcca cttccattaa gaaacgaaca ccgccaagca agtccaagcc tcgcttcatc
    10381 gggctaccgc caatgtcaga caaccactgt ggcgttgctg gtgcaaggtt taaggcaaca
    10441 gagtagttat tccccaatgc ttctgagatt ttttctttgg caggcaattg ctgttcgctg
    10501 ctttcgagac gaaccaaaat cgagccattt tctaacacgg cagatttcgg ctgaatattc
    10561 attgctgaaa gtgtggattg cacttgagac agtgtctcag gtgtggcttg ttgcccacga
    10621 gtaccggaaa tttgtaccga aggatcatcg ccatataaat ttggaagggc atataaagca
    10681 ccaatggcga tcacgaagat caccattaaa ttcttccata gagggaaacg gtttaacaca
    10741 ataactcccc gaatgttaaa gttggataag agggttgatt aaagtgattt taatgaacct
    10801 tttggtaaca ccgcaacaac gaaatcacgt ttgatggtca cttcagttgt gtcatttaat
    10861 gcaatcacga tgttatcgtt atcagcggtc actttggtaa ttttaccgat taaaccaccg
    10921 ctggttaata cttcatcgcc ttttgtcaag cttgccagta aatcacgttg ttgtttttgg
    10981 cgttttgcct gaggacgata aatcataaaa tagaaaattg caccgaaaat aatcaggata
    11041 aagatcattt ccattccgcc accttgttgc ataaaaactc cttaaatgtt tgagtggtat
    11101 aaaaataagt gttataaaaa acgcctgtta aaataccata aattcaatga attaacgagt
    11161 agaaaaattc atcgaatgct tttcttttaa acaaaaattt gagtagaatc agcactttta
    11221 gtttaggatt aggttcttgg cgtgttcttg cgctgacaca ataggagaaa aaatgaaaaa
    11281 attattcgct caagcggtta tttttggggc tttttttgca agttccgctt ttgctcacaa
    11341 tgtgcaatta aatcaagcat tacctgctgt ttcggttgca aaagacgggg aattggcagt
    11401 ggcaggtgga aaggttagct ataaaaattg gcattcatca tctctggcag gtaaagtccg
    11461 tgttattcat cattttgccg gtagaagttc ggtgaaagaa aaaaatgaag atttgatgac
    11521 agcaattaaa aatgccggtt ttgatcgctc gaaataccaa acggtgacga ttgtaaatgc
    11581 tgatgatgcg attgtcggca caggggtatt tgtgaaaaat agtgtggaaa acggcaaact
    11641 tgacaatcct cacagccaag tagtgttaga ccagcaaggc agtgtgaaaa aagcgtgggg
    11701 cttaaaagag aaagagagct ttattgcagt gctggataaa tcgggtaagg tacaatttac
    11761 ctctgagggc aaactctctg caaaacagat tcaagaagtg attgggttgg tgaaaacatt
    11821 aacgcaataa ccagtgtttt tatgggcgga ttttgtatcc gcccatcatt ctagataata
    11881 aatcgataac ttatcgtgtg atgatactct tctcctgctt ttgttatccc gccaaattgg
    11941 agtaattcag gctggtttag tgaatttggg aaaaactcag gctctaatgc tacaccagcg
    12001 taatcttcat attcgccttt agtcaaatta ggttgtcctt ttagccagtt gccggtgtaa
    12061 agttgtaatg ctggcatgga ggtgcggagt tccattttta agtcttccac ggctaaacaa
    12121 gcggtcggat tttcactatt ttttgcaaga tgaaaggagt gatcgtaacc ttttaccgct
    12181 ttttggtcgt catcagccaa taaatcttgc ccaatttttt tagcattgcg gaagtcaaaa
    12241 ctggtactct ccactttttt cagtggagca ttaggaatac cgctggcatc aaccggtaag
    12301 tatccgtctg cattaattat cagctcgtga tctaaaatcg tctctgaacc ttgcaaatta
    12361 aaataggcgt gattagttaa gcaaagcggt gtatcttggt tagcaacggc atcaaagctg
    12421 atttgaagct cattctctgt taagcgatac tccacaaaag ctttcacttc gccgccaaat
    12481 ccttcctcgc catctgcaaa aaaataacta aatttgaccg cttgttcgct ttggctttca
    12541 agttgccaca ctattttatc tgccccaagc tcgccaccgt ggagattatg ttcaccgtta
    12601 tttttagcga gctggtaggt tttgccgttt aagcgatatt cggcattggc aatgcggtta
    12661 gcgtaacgcc caatggtcgc tccaaagaaa gcggtctgtt tttgccaatt ttttgcagac
    12721 gtggtgacta atacttcccg ttcctcgttc ggcattttga caatgcacga aagccaagaa
    12781 gccccaacat cagaaagggt aatgcgtaaa aacgcatttt caagggtgaa taatttcatc
    12841 ggttgacctt aattacctag cacggcatag ccgtactagg ttaagcataa ttcagccctg
    12901 ctagggctga ggttatttat tttagagtag cggagctact tccttatgct taacccggta
    12961 cgctttagcg tgccgggcat tctcgcaccc catcggaggc agtacaaacg tggaaggttt
    13021 ctttaatgcc ggtttgcttt tcgtaattgt tagcaacggc ttggcggaga tcttccactt
    13081 tgtcatttgg cactaggcac acgacacaac cgccaaagcc gccgccagtc attctggctc
    13141 cgccgttttt accgatagct tgttgggcga tttcggctaa gcaatccact tcagggcagg
    13201 tgatttcaaa atcatcacgc attgagtcgt gagattgagc catcaactcc ccgagtttga
    13261 ccatatcatt cgcttttaag gcctctactg cttccagaac tcgttgattt tcgctgatga
    13321 tatgttttgc tcgtttatac gccagctcat tctcttcact aagttctgag gctcgttcaa
    13381 taaattgttc cgctgtgaca tcacgcaatt ttgctttgcc gaagaagtgg gcggcaaatt
    13441 cgcactgttc acggcgactg ttgtattcgc cggtaaccaa gtcgtgtttt acgttggagt
    13501 taataatcgc aatcgaatag ccttgtggca caggggttgg ggtgatttct aaactacggc
    13561 agtcgatcat cactaaatga tcttgttgcc cgagagcgga ggtgagctga tccatattgc
    13621 cgcagttgca gccgacaaat ttattctcag cctcttgccc gattaatgcg atttgcggta
    13681 aagaaagcgg taaatcgccg agtaattgag cggttttacc aatcgacact tccaatgcgg
    13741 cggaagaact taaacctgat gacatcggca catcgctggt aatggctaaa tctgctcctt
    13801 gtttgaattc ggggtgtttg gcttgaataa atttcaccac gccacgcaca taattcgccc
    13861 atttttgctc gcttggctca atcgcctggc ttaaatcaaa ttcatcttgt tggttgagat
    13921 caatggcgaa cacccgccat ttgttgtcac tacgtttggc aaagctaacc gctgtgccgt
    13981 aattgatggc acaaggcatt acaaacccat cgttgtaatc agtatgttcg ccgataatat
    14041 tcactctgcc cggggcaaat accgtgcgtt ctgctttata accaaaatgt tgttcaaatt
    14101 gttgcaaagc gatttcttgt ggtttcattt caattcctta tttatcttta tagtgtttgg
    14161 tatcacttac tgcatttaaa cgttcggcag cttgttccgg ggttaaatca cgttggcttt
    14221 ctgccatcat ttcataaccg accataaatt tacgcactgt tgccgaacgc aagagcggcg
    14281 gatagaagtg agcgtgtaat tgccagtgag cattttcctt gccgttaaac ggagcgaagt
    14341 gaaagcccat tgaataagga aagctgatgt taaacagatt gtcgtaacgg gtggtgagct
    14401 ttttcaatgc aagggctaaa tccgcttttt ccgcttcgtt taaatcggta atggatttga
    14461 actgcttggc tttcggcaac agcaaggttt caaacggcca agccgcccag taaggcacaa
    14521 ccgcaatcca atcttcggtt tctgccacaa ttcgctcttg tttgtctaat tcacgttgtg
    14581 cgtagtcaag caacagtggc gagccatatt gagcaaaata tttcgcttgg cattcgtctt
    14641 caatcgcaat ttcattaggt agaaaattac ttgcccaaat ttgcccgtgt gggtgagggt
    14701 tagaacaccc catcattgag cctttgttct caaaaatttg cacccactga taatgctctt
    14761 tcagctcatt cgcttgctct tgccaaactg ttaccacatc agtaatttcc tcgatggaaa
    14821 gttgcggtaa ggttttacta tgatcaggcg aaaaacagat cacacggctt tcgccttgtg
    14881 tatgagaaat ctgaaacagc ggatcatcat tcggtattgg tgccggtgtg tctggtagca
    14941 gggcagagaa gtcgttttta aacacaaacg gcttggtgta attcgggttt tgttcgccgg
    15001 taatccgctt attgccgggg cagaggtaac aacttgggtc gtagctcggt ttttcatctt
    15061 gcacgatttc ttcttgttgc ccttgccacg ggcgttttgc ccgatgcgga gagactaaaa
    15121 tccattgatt ttttaatgga ttaaaacgac gatgtgggtg atcgttgagg acaaattcgc
    15181 tcatataaaa ctcctcgaaa ttagttatgt aaagagtata gcgaaagtca gacaaaatga
    15241 ctgtgacata gctcacaaaa taatgatgtc aagcggtcaa atttcagaaa aattttgcaa
    15301 gttttcctcc attttgcgat ttaaggtagc ttacgtggaa ttgaatgtgg cttttggtag
    15361 aatagatgaa tatgcaagat attaagacaa aaatagatgg aaagactgaa taaatttgat
    15421 gcttggttaa tgaatcataa acctaatgct aggttaagcg gagcgagtcg tttgttgaca
    15481 gagttttggt tctttgggtt aaaagaagct agagcctgtt tatttgcggg attatttttt
    15541 attgcgatgt tttcggtacc aaaaacggga ttatggggct tacctcgtta tgatattttg
    15601 ctgatttttg ccatagtggt gcaaattgca atggtgaaac tggggctgga aacgtgggac
    15661 gagctaaaag caataagcct atttcatttg gttggttttg tgttagaagt ctttaaaaca
    15721 tcgagtaata ttcaatcttg ggcatatccg gattttgcct atactaaatt gttcaatgtg
    15781 ccattattta ctggttttat gtatgcagca gttggttctt acattatcca agcgtggcgg
    15841 ttgtttgatt tgaaaattaa aagccacctg ccctattggt tagccacttt aacggcactg
    15901 atgatttacc tgaatttctt tacgcatcat tacattgggg attaccgttg gtatattgcc
    15961 gcttttgcat tagggctgta tgcacgtact tttgtatttt tcacccctta tgacattaca
    16021 cgaaaaatgc cactgctgct ttctttcttc ctgataggtt ttttcatttg gttggcggaa
    16081 aatatcggta cgtttgccgg aatttggcgc tacccgaatc agtttggggc ttggtctatg
    16141 gtgcatatga gcaagtggag tgcttgggcg ttattggtga tgatgacttt taccatcatt
    16201 gtgcatttaa agcatatcaa gcaaacagtc agagtctcga gcgattaaca agctgtgggt
    16261 tttggcgaaa attttactca tcagttattt tcagtggaat taggggcaaa taataatttg
    16321 cccctatatc aaattatgga ttttcccctt ccacatcaat aatcatcact tccgactgtg
    16381 agcctaaccg catcggcacg ccccagaatc cgtagccgga ggtgacaaaa taatgcccga
    16441 agccgatttt ttcatagccg taatgtaatc gatagatcag tttggtaata atacttgcag
    16501 ggaacacttg ccctttgtga gcgtgtcctg aaacttgaat atcaatcggc aatttagcat
    16561 gttgctcaat ttcagtcggg cggtggtcaa gcaaaattac cggtagttta gtgttcacgt
    16621 tttgtaaaag ctgttcggca gaaggtcgat ttttgactaa atcatcatta cgcccaataa
    16681 gggtaaattt tccgttaatt tcggccgttt gatcccataa aacagtaata ccggcgtcgg
    16741 taatttcttt tgcaatcgtt tcttgagccc caaaaaagtc gtgattgcca agggtagcat
    16801 acacgccaag aggagctttt aatttggcta aatgaggctg cattttatcg gcaacataaa
    16861 cttcggtatt gtcgtccata atatcgcccg gcaataaaat cagatcgact ttttgctggt
    16921 tgaaaatctc tgccagttgg tcaagttgct tggttccgaa cagttttcct aaatgcagat
    16981 cgcttgccat accaatgcga agcggtttct ctaatggttt atccaatttc accgaataat
    17041 gtactactgt tggggtataa gcgttgtaat agccaagccc aagtaagcca aagaatgcaa
    17101 atgggtaggc gattttcagc cagcgattga cggtagtatt gccgttgaat attttgtaaa
    17161 tacaagcggt gaaaaaactg ataaaaaatg caaaaagtaa caataccaat atgaatgcca
    17221 ttattctaaa tagcgcaaaa gtacgagtaa gatgcaaaat cactaatccg tttgcgacta
    17281 agaaagtgag aataccaact gttttacggt ttttacttga taaattaaat aaccagctga
    17341 cagtctttgc aaagttgaat aaaaagagtt gtaacaccgc aatcacaagg gcggtggtaa
    17401 tgtagtaacg atattccata tttttccttc aataaattgg ctaaacccag ccgatttgtc
    17461 ttaataaaaa taagccgatg ctggcactaa acattgagcc gagagtcgta atgccgatca
    17521 gatttgcggc tgcctcgccg tctccgccat agttgcgtgt cattgcgtag gtggctgaag
    17581 caacgggagc agaagcagcc acaaagacaa tgcctagttc catcggcgga agttgaaaca
    17641 caaatttccc gagtagtagc aagaaaattg gagcaaaaat cagccgaatc gaggcagaaa
    17701 acagcacaat tttattgatg gtgctttcca ctccttgatt aaattgtttc aactgtttga
    17761 aattaatgct tgctccggtg cagataagtg ctaatggtag tgtcaggctc gccattaatt
    17821 taccagcaga ctcaagcggg gttggaattt ggtggcttaa ttcaaaatag ttaaaagtta
    17881 agcctgcaat gacggctaga atcagcggat ttctcaatat cgacaatatt aatctgccaa
    17941 aattcacttt tttctcgctt aacgatgaag tgagggtaat cactgcaagc acattaaagg
    18001 taatcaccaa gcacgaaata taaactgaag cggcagcgag agctttatcg ccataagcat
    18061 tgagaattaa ggcaatgcct aaaatcgccg cattggtacg gaaagtgcct tgagtgaaaa
    18121 tacaacgata acggcggttt tcaatatgtc gagccgccca ccattccgcc cataaataga
    18181 tcaaaatcga accgccgact ccggtcgaga tcaggttgat ttggctggag aaatctaacg
    18241 ggctttttag catattcaag aaaatcatcg gtggcagggt aaaattaaat accacctttg
    18301 atgccatatt gcaaaaggta tcgtcaataa attttcgtct gcgtggaaaa ataccgaaca
    18361 gcattaacaa aatggttggt aaggtaacgc caatgctaaa ttggatagag tcgaaaagca
    18421 taatgtttgc cttaaatgat ggcacgagtg tcttttgctt gtaccgtaga ttatcaataa
    18481 agtaccagcg tggacgctgg tactatcatc aatggaaatg aaattacaat cctgccttta
    18541 agcttgcttc gacaaattta tccaaatcac cgtccagcac ttgttgggtg gtgcgatgtt
    18601 ccacaccggt acggtggtct ttaatccgtg aatcgtctag cacataagag cggatctggc
    18661 tgccccagcc gatgtcggat ttgttgtctt ccatcgcctg tttttccgca tttttcttgc
    18721 tcatttccag ctcatatagc ttcgctttaa gctgtttcat cgcctgatct ttattttgat
    18781 gttgtgaacg accgttctgg cactgcacca caatgccgct cggtatatgg gtaattcgca
    18841 ccgccgattc ggttttattg acgtgctgac cacccgcccc tgaggcacga tacacatcaa
    18901 tccgcaaatc cgcagggttg atttcaatat caatatcgtc atcgacttcc ggataaacga
    18961 aagccgccga gaacgaggta tgacgaccgt tattggcatc aaacgggctt ttacgcacca
    19021 agcggtgaat acccgtttcg gttctgaacc agccgtaagc atagtcgccg gaaattttca
    19081 cggtggcgga tttaatccct gccacatcac cgtctgaggc ttccattagt tcggctttaa
    19141 accctttgct ctctgcccaa cgcaaataca tacgcagtaa catttctgtc cagtcctgag
    19201 cttccgttcc acccgaaccg gcttgcaaat ccaaatagca atcggcactg tcgtgtgggc
    19261 cgctgaacat ccgcttaaat tctaaatcgg caagtttttt ctctaattcg tctgcttcca
    19321 cttgggcttc attgaaggta tcctgatctt ctgcttccac cgccagctca atcaaccctt
    19381 caacatcttc aatcccttgc tctaaggctt taatcgtatt gacgatctgc tccaacattg
    19441 cccgctcttt accaagcgct tgtgcttttt ccggcttgtt ccatacatcg ggttgttcca
    19501 gctcggcatt gacctcttct aagcgttcga ctttatgctc aaagtcaaag ataccccctg
    19561 atcaccgcgg tgcgctcgct taaatcagtt agttggtttt taattgggtt taattcaaac
    19621 attgtcgttc ctgtttttgc ttattttaaa aagatgaatt atagctgaag ttaagcaagc
    19681 ggtcgaattt tgcaagaaat ggagcaatga tcgtttctag taatgagatt tggttcataa
    19741 aagttgcaac tgtttctgct atttggcttt gatagctaaa aatctttaaa aaaacaagcg
    19801 gtcgttttac cccatttttt gcaaaacgac cgtagcttct tgctataatc gctgaaaaaa
    19861 tgctgcaagg agattttatg gcaaagcgcc caagtgatat tgatgcaacc ctattgcaac
    19921 tggaaatcct acgccgtatc cccaaattac ccgctaaaat tgatgctaaa agtttgcacc
    19981 aacagctatt agatgccggc tttgaacggg atattcgcac tgttcagcgt tcgctcaaaa
    20041 tgttatgcga gcattttgat attgaatgcg atgagcggag caaacccttt ggttatagct
    20101 ggaaaatccg ctcagagggg ctggcattgc cgattctcaa cgaacaacaa tcattactgc
    20161 taaaacttgc cgagcaacag ctgaaatatc tgttacccgc aaatttaatg gcatcaatga
    20221 aacccttttt tgagcaggca gacagaatgg tttccggggc gaaagacaag cccgaatacc
    20281 aatggctaaa taaagtttgc tccacgccaa ccagcctacc gctgatccct gcaaaaatta
    20341 aagaagagat ttttaattcc gtaagccaag ccttatttca aaataagttg ttaaagatcg
    20401 aatatcagaa ccagcacggc aaaaaacacc aagcgacaat tatgccgttg gcgatttcac
    20461 aacaaggtgc gagcatttat ttggtctgcc gttacgaagg gtttgacgac aaccgcctgt
    20521 tggctcttca ccgcattcgt aaagcggaga tttctacttt tagttttgaa cgcccgaaag
    20581 attttaattt gcaagcctac caagatgccg gacacctcgg ttttggcaat ggcgaaaaag
    20641 ctagccttac cttttcgatt agcagagacg ccggtttcca tttaaccgaa acaccgcttg
    20701 ccaaagatca aaaaattctc gaggagagtg aaactcacta ccgtattcaa gccatagtcg
    20761 ctgataatga tatgctaacc tggtagctac gcaaatttgg cgaagatatt tgggatatcg
    20821 agaaagaagc gctaaaataa gctaaacaag cggtcggttt tgtgggaatt tttgctaaat
    20881 agtatcgacc gctcctccct aaacagacct ttatcataaa tacaactctt tcaatcagat
    20941 attcttactg tctattgatg aacgattcga atttttctag tggttgaaat ttaaactaat
    21001 tttcttgctt tttggcattt ttcagacttt taattctctc cattagcctg tttttttacg
    21061 ctctttttca taaacaggaa taaccaatgc cttttcaact tactttctgc caacaagccg
    21121 gcaatcagca ccagaaaaat caagatgcac tgtttaacgg caaagcggtg taccaatggc
    21181 aacttaaaaa tgccgagagt tatctgctgg ataacgacaa agtaattttt ggggttgcgg
    21241 acggcgtttc cagccaccct cgaaatcatc ttgcctcaca ttatttaatg gcgttactca
    21301 gccaatgcca atcccttgat agccaatggt tgcgacaggc tcacggcgat ttttgcgaaa
    21361 aattagcgaa agattatttt ggtgcttctt gcacttttgt tgcttgtgaa ttacaccata
    21421 acggcagagg gcgtatggtt aatgtgggcg acagtcgagc ctataaaatt acggcaaacg
    21481 gggagtggca acaactgagt tttgatcaca ccatactggc tgaaatgaaa cagaaaggct
    21541 tggttaagga agggagcaac tatgcctcaa tgtataacgg cttaaccgat tgtattgttg
    21601 ccgattttga cgccgatgat ttccgcattc atatagccga atttcaactg gaaaaaggcg
    21661 agagcatttt gctctgttct gacggtttgc acgaacaact cgccccagaa gctattcagg
    21721 cgatttagga cggatacgaa agcccgtctc atcgtctgca aatttgcaga aaaatggtaa
    21781 aaatgcaccg cttgtatgac gatttttctg cggtgatttg tgaaattgtg taattaaaac
    21841 gacattattt gtcgcattgg tgcttaaaat ccgcataaaa cagaggagaa aaaaatgaaa
    21901 aacgatttcc attatgccgc tgaattaatc cgcaatgcag acggcatttt aattaccgca
    21961 ggggctggaa tgtcggtgga cagtggcttg cccgatttcc gtagcgtggg agggttatgg
    22021 aattcctacc caccgctcaa aaaatttaat ttgcaattta tgcagattgc cacaccgctt
    22081 gcctatcagg aacgccctga
//
LOCUS       DANLUQ010000023        21233 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.023, whole genome shotgun sequence.
ACCESSION   DANLUQ010000023 DANLUQ010000000
VERSION     DANLUQ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 21233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21233
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.023"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..358
                     /locus_tag="PXB27_000728"
     CDS             <1..358
                     /locus_tag="PXB27_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5345547.1"
                     /translation="ELVSVSFFVNNLIAVLLQSDFSRKLNLTVHKLIYIAPILIILQI
                     MPWFYALQWLSTITAFLYLVIFATITVYANTALSNMLVKLDNGKNQGLMFGSSKLILA
                     ITTLGVMNVLPYIFLI"
     gene            complement(355..1488)
                     /locus_tag="PXB27_000729"
     CDS             complement(355..1488)
                     /locus_tag="PXB27_000729"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HDL5345548.1"
                     /translation="MLIFMMNEYSPNYYIGVMSGTSLDGVDIALLDFAKNPPKMTACD
                     FFPMPEELRADISALLKTGETNLQKLGEIDHRLGLLYAETINAFLAKNQLKAEDIQAI
                     GCHGQTVWHSPDSEYPFTTQIGDMNLVAEQTGITTIADFRRKDMAVGGQGAPLVPAFH
                     QAIFSDPNRLTVVLNIGGISNISVLNPNSPTVGYDVGAGNTLMDSWIEQHQGKRYDKN
                     GEWARTGKLNTELLATLLDESFFAKAPPKSTGRELFNLTWLAKKLENLTACQPQDVQR
                     TLAEFTAQSIANELLRFESSLPRLLLVCGGGSRNPLLMERISALLPEWEVTTTTEFGL
                     DIDYVEAAAFAWLAYQRIHNLPSNMPSVTGAKKAVSLGVIYPA"
     gene            complement(1680..2519)
                     /gene="queF"
                     /locus_tag="PXB27_000730"
     CDS             complement(1680..2519)
                     /gene="queF"
                     /locus_tag="PXB27_000730"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HDL5345549.1"
                     /translation="MNYNDKSLSALKLGQKTEYKSEYDASLLQPVPRKLNRDSLGITQ
                     EQPFNRGADVWTCYELSWLNQNGLPQVAIADVAIDFKSENLIESKSFKLYLNSFNQTK
                     FSSIEEVGKTIQADLARCASGQVSVKIYQLSHYTQQPIVDFAGDCIDEQDIQIDSYAF
                     STHYLENIAEDELVEETLVSHLLKSNCLITSQPDWGSVQIRYKGKKLNREKLLRYLVS
                     FREHNEFHEQCVERIFCDLMQFAEPEKLTVYARYTRRGGLDINPFRSNFEEVPENLRM
                     ARQ"
     gene            complement(2524..3072)
                     /gene="gmhB"
                     /locus_tag="PXB27_000731"
     CDS             complement(2524..3072)
                     /gene="gmhB"
                     /locus_tag="PXB27_000731"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HDL5345550.1"
                     /translation="MGKKAIFLDRDGTINIDHGYVHQIDDFQFIDGVGKALKQLQDKG
                     YLLVLVTNQSGIARGYFSEAQFMQLTEWMDWSLDEDYGVVLDGIYYCPHHPEGKGEYR
                     KDCDCRKPKAGMFTQAIQDLNIDPSQSYMVGDKLEDILAAEAAGVKTKVLVRTDKAVT
                     EEGQAKADLVLDSLVDLVKYIK"
     gene            complement(3072..3539)
                     /gene="bcp"
                     /locus_tag="PXB27_000732"
     CDS             complement(3072..3539)
                     /gene="bcp"
                     /locus_tag="PXB27_000732"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HDL5345551.1"
                     /translation="MNILQAGDKAPKFTLQNQADEPVSLSQFAGKKVLVYFYPKALTP
                     GCTTQACGLRDSKSELDELNVVVLGISTDLPKKLAQFVEKKALNFTLLSDPDHQVAEA
                     FGVWGEKKFMGKTYDGIHRISFLIDEQGGVEQVFDKFKTGEHHQMIVDYLRGR"
     gene            complement(3626..3823)
                     /locus_tag="PXB27_000733"
     CDS             complement(3626..3823)
                     /locus_tag="PXB27_000733"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345552.1"
                     /translation="MKFGYLNGVLEGKGKMRFDEAKLHAESEFEKYRIVQDRLFESDF
                     DKVLKEMLRSENQYRPSFSTT"
     gene            complement(3792..4274)
                     /locus_tag="PXB27_000734"
     CDS             complement(3792..4274)
                     /locus_tag="PXB27_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HDL5345553.1"
                     /translation="MIDKQIRNSTAEFLIFTQQNGAENITVRYEDNEIWLTQKLIAQL
                     FDCSTDNVSLHLKNIFNSRELDPNSVTEDFSVTAADVKNYRTKHYNFDAVISIGYRVN
                     SVRATQFRQWATKVLRNFAQKGYVIDRQRMENGTFLNEDYFEQLLAEIREIWLSERRS
                     "
     gene            complement(4417..5574)
                     /gene="hemW"
                     /locus_tag="PXB27_000735"
     CDS             complement(4417..5574)
                     /gene="hemW"
                     /locus_tag="PXB27_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HDL5345554.1"
                     /translation="MNLILPPLSLYIHIPWCVQKCPYCDFNSHAQKGLIPEQEYIEHL
                     LADLSQDLTAYRVAIGDRKLYSIFIGGGTPSLFSAEGIAYLLIEVEKRIAFEPNIEIT
                     LEANPGTAEAERFLGYAQGGVTRISMGIQSFEPEKLLKLGRIHDANEAKQAIKFAQNS
                     AKSGLQSFNIDLMHGLPNQSVKQALADLETGISLNPPHLSWYQLTIEPNTMFYYRKPT
                     LPDDDELWDIFEQGHQLLTNAGYEQYETSAYAKKGYQCRHNLNYWRFGDYLAIGCGAH
                     GKISYPTGEIYRFSKIKHPKGYMRGEYRYSQDLIELDDRPFEFFMNRFRLLEATPKQE
                     FEWFTGLDREVVRPTIDWALNQNYISETATHWQITQHGKLFLNELLEGFLE"
     gene            5796..7379
                     /locus_tag="PXB27_000736"
     CDS             5796..7379
                     /locus_tag="PXB27_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5345555.1"
                     /translation="MKKQFEHNESRRGFMKLIAGVGAGMAFSGTLGTFTPKAFAAPAA
                     GSTIEAGIAYPISTGFDPLTSSGASSMAANLHIFEGLVDLHPATRKPYLALAAAEPEK
                     IDDVTYRITLRDGAKFHNGNPVTTEDVVYSFERVLDPAKASLFAQFIPFIDTVKKVDD
                     KVVEFKLKYPFALFKERLTIVKIVPKAVVEAGQAAFDANPVGTGPYKFVSATKDDRIV
                     FEAFADYNGGYPAQVEKMTWFLLSDDAARVTAQESGRVQAIESVPYLDAERLKRKGTV
                     ESVQSFGLLFLMFNCEKAPFNNPKVRQALHYGLDTQKLIDVVFLGNAKAATSYVQDTH
                     PDYVKANSQYDFDKAKAEALLAEAGVTELKFELLATDHSWVKECAPLILESWNSLKGV
                     KVSLKHLQSGALYGTHVDKGAYEVVIAPGDPSVFGNDLDLLLSWWYRGDVWPKRRFRW
                     SETAEYAEVQKLLDEAAKNPAASKEAWAKAINIIAEQVPLYPIIHRKLPTAWNDKALT
                     GFQPLPTTGMSFIGVGRAK"
     gene            7591..8544
                     /locus_tag="PXB27_000737"
     CDS             7591..8544
                     /locus_tag="PXB27_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5345556.1"
                     /translation="MEIILRLLWRRLMALPVMILGVTALVFIVLQFTPGDPATVALGE
                     SASEAAKEIYREQHGLNDPLLVQYFRFLGNLFVLDFGMTTPPEQPIVDMIAKAFPLTL
                     QLTFIGVFLAAIVSFTLGVTAALYRDTWIDQLIRLISVAAVATPSFWLGILLIQYFSL
                     KLDWLPSGGFIPFSEDPKGYVNSMVLPSLALAVPVCASLIRVVRTTMVEEMDKDYVRT
                     AIGNGVPYSTVIRHNVLRNALITPVTVLGLRVGYLLGGAVVIEQIFDLPGMGKLIFNG
                     IVNHDLNLVQGVVLTIAFTFVLVNIIVDILYLLINPKIRSL"
     gene            8546..10516
                     /locus_tag="PXB27_000738"
     CDS             8546..10516
                     /locus_tag="PXB27_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HDL5345557.1"
                     /translation="MFRQGLAARLASGGARFRALSTSSKIALLFIVFIALVAIFAPLV
                     APYDPLQTLRPVQAPSGDYLFGTDRLGRDIFSRMVWGARTSLFIGLGAVGVAILFGSI
                     LGATAATADKFGNEVIMRFMDILMAFPGIALAAVLLATFGNSVPVIIVTIAVVYTPQL
                     ARVVRANVVSQWEEDYVRAERVIGGSRTYILLKHVVRNTAAPVLVFATVMVADAIVFE
                     ASLSFLGAGVQPPFPSWGNILSEGRNLVLSGFWWATTFAGIMILLTVLALNILAEGLT
                     DALVNPKLKRSTQSKEDVAKPLATDVQEAMSETLALKRYLLKLHEKETTRTDRMQLNS
                     TAKPILQVKNLSIRFPNRYGETPLVDNISFTVHEGETMGLVGESGCGKSITAFSIMGL
                     LPKTAKITGEILFTDRSGKQHNLLESADLNALRGHEISMIYQDALSALNPSMRIKDQM
                     AQLISRGGKQSAETLLQWVKLDPEKTLNRYPHELSGGQRQRVLIAMALAREPKLLIAD
                     EPTTALDVTVQAEVIKLLNELREKLGFAMVFVSHDLALVAQIAHHITVMYAGQVVETA
                     PTTPLLANPTHEYTRGLLGSVLSTELRAERLYQIPGSVPSPFDFAKGDRFASRSLRPD
                     ANPEQHLKLVPTGDHPQHFWASHLEDPQSKEA"
     gene            10531..11337
                     /locus_tag="PXB27_000739"
     CDS             10531..11337
                     /locus_tag="PXB27_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5345558.1"
                     /translation="MSMKFIKEQPIIELSNIVVQFASRDGTLFNPKKFTAVNDVSLAI
                     HAGETVGLVGQSGCGKSTLASVMIGLQKPTSGTVKFNGLEMKYGSAEARKYFGSQVSV
                     IFQDPATALNPRMRVLDILKDPMDIHNILQPQEREKRVYDLLSRVGLPRSAALVEPTR
                     LSGGQKQRVAIARALALNPKLIVADEPTSALDVSVRAQVLNLLADLKKELNLAMVFIS
                     HDLQTVHQVSDRIVVMNGGKIVEMGDASDVFERPTQEYTRTLIEAAPSLL"
     gene            11400..12986
                     /locus_tag="PXB27_000740"
     CDS             11400..12986
                     /locus_tag="PXB27_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HDL5345559.1"
                     /translation="MIQITQTINIDLLIVGSGIAGLSAAVEAKEKGINTLLISKSTIG
                     SGASYFPLKATLGIQVTGDEADKIHFKEDIENIGKGKINPNVVQAYIEDSPQAIELLH
                     RIGFQPWKRNDNRPACFAKYPRPIYLIKDWRESALRAKQILQEQHIAFYENATLLHIV
                     TDNNQVQGAVFSLTTSGQISYIFCKTKHLILAAGGIAGLYKDNLYPADVIGSLHYVAQ
                     QAGATLVNLAFIQFIPAFVEPKYKVLFGEHTLKYVTQITDSKGNNLFPHLNAEQFAKM
                     VLERSHYAPFSLDFAGVEFDLVMMKHLLENPHEKGIYLKYSPKLYEDTEEFYTVYLNW
                     LKNEVGIDLVQDKIAIKPFAHSCNGGIQINQWGESAVKGLFAIGEISHCIEGANRMGG
                     NSVGGGLVFAKRAVSEIVRSLEQNDLQKNSEISPLAMAEKALNQLANSTANLELTASE
                     VLSTIREKMALYANVYRTRSNLKLLLEELNRLEQEFDPIYHAQYQGIEIYNALKTAQL
                     VVNQMLNEQSLGAHYLDEQN"
     gene            13032..14414
                     /gene="nhaC"
                     /locus_tag="PXB27_000741"
     CDS             13032..14414
                     /gene="nhaC"
                     /locus_tag="PXB27_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL5345560.1"
                     /translation="MNNYLLKLKPMTAVILSLLTVFILGFTMIGLGWVPQISILAVIV
                     MLLLFGLINKIPFKNMQEGMASGLMSSIGAVYLFFFIGLLVSALMMSGAIPTLMYYGL
                     SVISPELFYISAFALSSVVGIALGSSLTTTATIGVAFIGMASAFGANPAIVAGAVVSG
                     AFFGDKTSPLSDTTTIAASVVGIDMFAHIRNMLYTTIPAWILTALFLWFLTEPTDAQN
                     LANIENLKTNLQETGLIHGYSLLPFVTLILMAIKRVNSIYIIVTTVTLSLIITYIHSS
                     PTLEQLGNYFFTSYKPVEGVDLGEVASLVSRGGINSMFFTITIVLLALSLGGLLQILG
                     ILPALLNGISGFLTSTGRVTLVVASTAVGINVLIGEQYLSLLLSGNTFRSTYERLGLE
                     VKNLSRTIEDAGTVINPLVPWGVCGVFISSVLNVPVLDYLPYAFFCYICLILTLIFGF
                     TGVTISKKAN"
     gene            complement(14457..15653)
                     /gene="ispC"
                     /locus_tag="PXB27_000742"
     CDS             complement(14457..15653)
                     /gene="ispC"
                     /locus_tag="PXB27_000742"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HDL5345561.1"
                     /translation="MKKLVILGSTGSIGKSTLSVVEHNPNPNQYEVFALVGGKNVELM
                     LEQCRQYQPTFAAMDDEKSAQALKTALATLGSKTEVLAGQAAICELAAHSEVDMVMAA
                     IVGAAGLLPTLSAVKAGKTVLLANKESLVTCGQIFIDEARKSGAKLLPVDSEHNAIFQ
                     SLPPEAQQKVGFCPLAELGVSKIILTGSGGPFRTKPLNEFDAITPAQAVAHPNWSMGK
                     KISVDSATMMNKGLEYIEARWLFNATAEEMDIIIHPQSIIHSMVRYIDGSVIAQMGNP
                     DMCTPIAHTMAYPNRINAGVPPLDFFKLKELTFIEPDFARYPNLKLAIEAFAEGQYAT
                     TAMNAANEIAVEAFLNEQIRFTDIVNVNRTVVENIAPIQVSEIADVLHIDKLARELAK
                     QAVLQL"
     gene            complement(15772..17970)
                     /gene="relA"
                     /locus_tag="PXB27_000743"
     CDS             complement(15772..17970)
                     /gene="relA"
                     /locus_tag="PXB27_000743"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HDL5345562.1"
                     /translation="MVAIRYSHQLEPNNFELSSWCASLNMSPVTFEQIHNAWSYAQEK
                     LDTDYSLMWYGIEMVEVLHGLNMDDDSLVAAMLFPLVKHNVIDLVQLKEDFSNDIKNL
                     VKGVLEMENIRQLSANNVSDLQIDNIRRMLLAMVDDFRCVVIKLAERIAYLRDSNHHS
                     EEDTVLAAKECSHVYAPLANRLGIGQLKWELEDYCFRILHPVSYTQIAKFELGERRVD
                     RERYIANFVADLTACLSPEIKNVQIYGRPKHIYSIWKKMQKKRLRFNQLFDIRAVRVI
                     VPTLEDCYTALGIIHTKYTHLPEHFDDYIANPKPNGYQSIHTVVLGEGEKTIEVQIRT
                     QQMHDDAELGVAAHWKYKEGATAGRSGYEEKIVWLRKLLEWQNDLADSGDMVAEMRSQ
                     IFDDRVYVFTPRGEVIDLPQKATPLDFAYAIHSEIGHRCIGAKVAGRIVPFTYHLQMG
                     DQVEIITQKEPNPSRDWLNPNTGFVNTGKARSKIIAWFKKLDREKNIPIGKEALEAEM
                     EKFGFTLKQIEEYALPRYNLKQFDDLYAGIGGGDIRLHQLMHFLQSKLIKPTAEQEDE
                     AVLKQVNKNTNQPKPSKGYVIVEGVGNLMHSIARCCQPIPGDEIVGYITQGRGISIHR
                     ADCEQLFELQSLNPERVVEADWGEGYTSGLSLTIRVIANDRNGLLRDVSAIMANEKVN
                     VLGVSSRTDVKRSLATIDMEIQLNNVEILNKLLARIAQLDDVIEAKRLSS"
     gene            18224..19531
                     /locus_tag="PXB27_000744"
     CDS             18224..19531
                     /locus_tag="PXB27_000744"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HDL5345563.1"
                     /translation="MTILALGINHKTASVGLREKVAFVDDKRKLALEQIQTSGLAESV
                     VILSTCNRTELYFHQPNISPREESEENIQWREQCFRWFAQIHQLDESELRECLYFKQN
                     LEAANHLMKVASGLDSLILGEPQILGQVKQAYQYSEDFYQHQQKAMSTKLSRLFQRTF
                     ATAKRVRSETEIGSQAVSVAYAACGLARQIFDNFGKLRFLLVGAGETIELVARYLIQH
                     GAKNIMLANRTHIRAEMLAEKLNAPMQILSLSALQLGLNQADVVISSTGSPDTLISKE
                     MVEIAQKQRQFDPLLLIDIAVPRDIDEKAGDLEGVYAYSVDDLQNIIQRNMAQREEAA
                     KQAAEIVKEESKAFFEWLKQQQSSDLIKRYRQDAEQTRQELLEKALQAISQGQDSQKV
                     LSELSYKLTNQLLHAPTQALQAMAKSGNSTGLQSFSRALRLEE"
     gene            19716..>21233
                     /gene="der"
                     /locus_tag="PXB27_000745"
     CDS             19716..>21233
                     /gene="der"
                     /locus_tag="PXB27_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDL5345564.1"
                     /translation="MTPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGH
                     ANIAGYDFIVIDTGGIDGTEEGVEEKMAEQSLLAIEEADVVLFLVDARAGLLPADVGI
                     AQYLRQREKTTVVVANKTDGIDADSHCAEFYQLGLGEVEQIAAAQGRGVTQLIEQVLA
                     PLGEQLNAEQFNQEQAGENAKNVANEETDEWDTEFDFENEEDAALLDEALAEEAEESI
                     EDKNIKIAIVGRPNVGKSTLTNRILGEERVVVYDMPGTTRDSIYIPMERDGQQYTIID
                     TAGVRKRGKVNLAVEKFSVIKTLQAIQDANVVLLTIEAREGISDQDLSLLGFILNAGR
                     SLVIVVNKWDGLSQDIKDQVKSELDRRLDFIDFARVHFISALHGSGVGNLFDSVKEAY
                     ACATQKTSTSMLTRILRMAADEHQPPLVNGRRVKLKYAHPGGYNPPIIVIHGNQVEKL
                     ADSYKRYLSNYFRKSLKIIGSPIRILFQEGNNPFAGKKNKLTPSQLRKRKRLMKFI"
BASE COUNT         6082 a         4574 c         4252 g         6325 t
ORIGIN      
        1 agaattagtc tctgtctcat tctttgtgaa taatttaatt gcagtgttat tgcaatctga
       61 tttttcacga aaattaaatc tgacagtaca taaacttatt tatattgcac caatactgat
      121 tatacttcaa ataatgcctt ggttttatgc actccaatgg ctttcaacca tcactgcatt
      181 tctctattta gtgatttttg ccactattac cgtgtatgcg aatacggcac tatctaatat
      241 gctagtgaaa ttagacaatg gtaaaaacca aggcttaatg tttggaagct ctaaattaat
      301 tttagctatt accactctcg gagtaatgaa tgtattacct tacattttct taatctaagc
      361 agggtaaatc acgcccaaac tcacggcttt ttttgctcct gtcacgctag gcatattact
      421 gggtaaatta tggattcgct ggtaagctaa ccaagcaaaa gcggcagcct ctacataatc
      481 aatatctaaa ccaaattctg tagtggttgt aacctcccac tcgggaagta aagcagaaat
      541 ccgttccatt aataacggat ttcttgaccc accaccacaa actaaaagca agcgaggcag
      601 agagctttca aagcgtaata actcattggc aatactttga gcagtaaatt ctgccagcgt
      661 gcgttgcaca tcttgtggct gacaagcggt caaattttcc aatttttttg caagccacgt
      721 taaattaaac agctcccgcc cagtgctttt aggcggggct ttagcaaaaa acgactcgtc
      781 taataacgtc gctaacagtt cagtattgag cttgcccgtt ctcgcccatt cgccgttttt
      841 atcgtatctt ttgccttgat gttgttcgat ccaactatcc atcaaagtat ttccggctcc
      901 tacatcataa ccaactgtag gggaattggg atttaacacc gaaatattgc tgatgccgcc
      961 aatattcagc accactgtta gacgatttgg atcgctaaaa atagcttgat gaaatgccgg
     1021 cacaagcgga gccccttgcc cacctactgc catatcttta cgccgaaaat cagcgatagt
     1081 ggtaataccg gtttgctctg ccaccaaatt catatcgcca atttgggtgg tgaagggata
     1141 ctcagaatcc ggtgaatgcc acaccgtctg cccgtgacaa cctatggctt gaatatcttc
     1201 tgcttttagc tgatttttcg ctaaaaacgc attgatcgtt tctgcataaa gcaagcctaa
     1261 acggtgatca atttccccca gtttttggag attggtttcg ccggttttaa gtaacgcaga
     1321 aatatccgcc cgtaattcct ccggcattgg aaaaaaatca caagcggtca ttttcggtgg
     1381 attttttgca aaatccagca aagcaatatc caccccatca aggctcgtgc cggacattac
     1441 gcctatatag taatttgggc tatattcatt catcataaaa atcaacatct gctatttgtt
     1501 acttttcttt gctttgccaa agaaaagtaa ccaaaagaaa ggcaacccga gcttcaccgc
     1561 tatcctcgct tagtttgaat tggcttaacg gcaaatttac attcgctccg ctcagagtaa
     1621 atttacctac caattcaaac acgctcaggc ggttcagacg ggaacaaaaa acaacaaaat
     1681 cattggcgtg ccattcttag attttccggc acttcctcaa aattcgatct aaacggattg
     1741 atgtctagcc cgcctcgtct ggtgtagcgt gcgtaaactg ttaatttttc cggctcggca
     1801 aattgcatta aatcacagaa aatgcgttcc acacattgtt cgtggaactc attatgctca
     1861 cggaacgaca ctaaataacg taacagtttt tcacggttta gctttttgcc tttgtagcga
     1921 atttgcacac ttccccagtc aggctgtgac gtaattaagc agttagattt gagcaaatgg
     1981 ctgactaagg tttcttccac caactcatct tctgcaatgt tctctaaata atgagtcgaa
     2041 aacgcataac tgtcgatttg aatatcttgc tcatcaatgc aatcgcctgc aaaatcaact
     2101 atcggctgtt gcgtgtagtg tgataattgg taaattttta ccgaaacctg accgcttgca
     2161 cagcgagcca gatccgcttg aatcgttttc ccaacttctt ctattgagct aaatttggtt
     2221 tggttaaaac tgttcaaata gagcttgaaa cttttcgatt caattaagtt ttcgctctta
     2281 aaatcaatgg cgacatctgc aatcgctact tgcggtagcc cattttggtt caaccaagat
     2341 agctcatagc aagtccacac atcagcccct cggttaaaag gctgctcttg tgtaatgcct
     2401 aaactatcac ggtttaattt acgaggaaca ggttgcaaaa gcgaggcatc atattcgctt
     2461 ttgtactcgg ttttttgccc taatttcagg gcggaaaggg atttatcgtt ataattcatt
     2521 gctttattta atatatttta ctaaatcaac taaactatct aacaccaaat cggctttagc
     2581 ctgaccctct tccgtcactg ctttgtcagt gcgaaccagc actttggttt tcacgccggc
     2641 ggcttctgcg gcaaggatat cttctaattt atcgcccacc atataagatt ggcttgggtc
     2701 gatgtttaaa tcttgaatcg cttgagtaaa catacctgct ttaggcttac ggcaatcaca
     2761 atctttacgg tattcgcctt tgccttcggg gtgatgtggg cagtagtaaa taccgtctag
     2821 taccacgcca taatcttcat cgagcgacca gtccatccat tcggttagtt gcataaattg
     2881 tgcttcgcta aaatagcctc gggcaatgcc agattgattg gttaccagca ccaataaata
     2941 acctttgtct tgtagctgtt ttaacgcctt gccgacaccg tcaataaatt gaaaatcatc
     3001 aatttgatgc acatagccgt gatcgatatt aattgtgccg tcacggtcta aaaaaatggc
     3061 tttttttccc attagcgacc ccgtagataa tccacgatca tttggtgatg ttcgccggtt
     3121 ttaaatttat caaacacttg ctcaaccccc ccctgctcat caattaaaaa actgatgcga
     3181 tgaatgccgt cataggtttt tcccataaat ttcttttcgc cccatacacc aaaggcttct
     3241 gcaacttggt gatccgggtc agataataag gtaaaattaa gtgctttttt ctctacaaat
     3301 tgagctaatt ttttcggtaa atcggtgcta attccgagaa caaccacatt gagctcatca
     3361 agttctgatt tgctgtcacg cagtccgcac gcttgcgtag tgcaaccggg tgtaagagct
     3421 ttcggataaa aataaaccag tacttttttg ccggcaaatt gagagagaga aaccggctcg
     3481 tcagcctgat tctgaagggt aaatttaggg gctttatcgc ctgcttgtaa gatattcatt
     3541 tgtaattttt ccataaaaaa taaccgcttg cattatacac ggcaagcggt caaacttggc
     3601 aaaaaatttg caaaatgcgg ttgttttatg ttgttgaaaa tgatggacgg tattgatttt
     3661 ccgaacgcaa catttctttc aataccttgt caaaatcgct ttcaaataat ctgtcttgaa
     3721 caatgcggta tttctcaaat tcgctttctg cgtgcaattt cgcttcatca aaacgcattt
     3781 tgcctttgcc ctcaagaacg ccgttcagat aaccaaattt cacgaatttc tgccagcaac
     3841 tgctcaaaat aatcttcatt taaaaatgta ccattttcca ttctttggcg gtcaatcaca
     3901 tagccttttt gggcgaaatt ccgtaatact ttagttgccc attggcgaaa ttgtgtcgcc
     3961 cgcaccgagt ttaccctata gcctatcgaa ataacggcat caaagttgta gtgtttggta
     4021 cgataatttt tgacatctgc ggcagttacc gagaaatcct cggtaactga atttggatcg
     4081 agttccctac tattaaaaat gttttttaaa tgcaaagaaa cattatctgt tgagcaatca
     4141 aaaagctgag caattaattt ttgcgtaagc caaatttcgt tatcttcata gcgaacggta
     4201 atattttctg ccccattttg ttgagtaaaa atcaaaaatt cggcggtact attacggatt
     4261 tgtttatcga tcataagctc tccttattgt gaaggctatg ctactaaata ctgttttttt
     4321 gtctagtaaa aaatgaggat ttttcgacgc agatcacaaa aaacgcctgt aatttagcat
     4381 tacaatgcgg tcttttttta gtcgtttttt tgcaaattat tccaaaaatc cttccagtaa
     4441 ttcatttaaa aacaatttac cgtgttgggt aatttgccaa tgggttgcgg tttcggaaat
     4501 atagttttgg tttaacgccc aatcgattgt cgggcgaaca acttccctgt ctaaccctgt
     4561 aaaccattca aattcttgtt ttggtgtcgc ttcaagtaat ctaaagcggt tcataaaaaa
     4621 ctcaaacggg cggtcgtcta attcaatcaa atcttggctg tagcgatatt cacctcgcat
     4681 ataacctttc gggtgtttga ttttgctaaa gcgataaatt tccccggtcg gatatgaaat
     4741 tttgccgtga gcgccacaac caatcgccaa ataatcccca aatcgccaat aatttaaatt
     4801 gtgtctgcat tggtagcctt ttttggcata agccgaggtt tcatactgct catagccggc
     4861 attcgttaaa agttgatgcc cttgctcgaa aatatcccat aactcgtcat catcaggcaa
     4921 ggttggctta cgataataga acatcgtatt cggctcaatc gtgagctgat accacgataa
     4981 atgcggtgga ttgagtgaaa tccctgtttc taaatccgcc aaggcttgtt taaccgattg
     5041 gtttggtaag ccgtgcatta aatcaatgtt gaaactttgc aagcccgatt ttgcagaatt
     5101 ttgggcaaat ttaatcgctt gttttgcttc atttgcatcg tgaatccgtc ctaatttcaa
     5161 taatttttcc ggctcaaaac tttggattcc cattgaaatg cgggttacgc caccttgtgc
     5221 gtaacctaaa aatcgctctg cctctgcggt tcccgggttc gcttccagtg taatttcgat
     5281 atttggctcg aatgcaatcc gtttttctac ttcaattaat aaataagcta tcccttccgc
     5341 tgaaaataag ctcggggtgc cgccgccaat aaaaatagaa tataatttac gatcacctat
     5401 tgccactcgg taagcggtca aatcttggga taaatctgca agtaaatgtt cgatatattc
     5461 ttgctcggga attaatcctt tttgagcgtg cgagttaaaa tcgcaataag ggcatttttg
     5521 cacgcaccaa gggatatgga tataaaggct taaaggcggt aaaatcaggt tcactgtcaa
     5581 tttttcattt atgattaaat tggcgaataa taacgaaaag cacgtgaagt gaaaagcaaa
     5641 gattttcgtg agctagatca caaattcaat aattcttgct ttaccacttt gacattaatg
     5701 atatgataaa ttcaattcaa aaaagtgcat tttttgataa attcacttca gcaaataata
     5761 ccgcccctat cataaaaact taaaggagct ttaagatgaa aaaacagttt gaacacaacg
     5821 aatcccgccg tggttttatg aaactaattg caggtgtcgg tgccggtatg gcatttagtg
     5881 gcactttagg tacatttaca cctaaagcat ttgcagcccc tgccgccggc tcaacgattg
     5941 aggccggtat agcttatccc atatcaaccg gatttgaccc gcttacctca agcggtgcat
     6001 cctcaatggc ggcaaacctc catatttttg aaggcctcgt ggacttacat ccagccactc
     6061 gcaaaccata tttagcattg gcagcagcag agcctgaaaa aattgatgat gtaacctatc
     6121 gaattacttt acgtgacggg gctaaattcc acaatggtaa tccggttacc actgaagacg
     6181 tagtttactc atttgaacgt gtattagacc cagctaaagc atcacttttc gcacaattca
     6241 ttccatttat tgacactgtg aaaaaagttg atgacaaagt ggttgaattc aaattgaaat
     6301 atccatttgc gttattcaaa gagcgcctaa ccattgttaa aatcgtacca aaagcagtgg
     6361 tagaagccgg tcaagctgca tttgatgcta atccagtggg gactggccca tataaatttg
     6421 tatccgcaac taaagatgat cgtattgttt tcgaagcatt tgcagattat aacggtggct
     6481 acccggcaca agtcgagaaa atgacttggt tcttactttc tgatgatgcc gctcgtgtaa
     6541 ccgctcaaga atctggccgt gttcaagcaa ttgagtctgt gccttactta gatgcagaac
     6601 gcttaaaacg taaaggtacc gttgagtcag tacaatcatt cggcttactg ttcttaatgt
     6661 ttaactgtga aaaagcaccg tttaacaacc cgaaagttcg ccaagcgtta cattatggct
     6721 tagataccca aaaattaatt gatgtggtgt tcttaggcaa tgcaaaagct gctacatctt
     6781 acgtgcaaga tactcacccg gattatgtga aagcaaattc gcaatatgat ttcgataaag
     6841 cgaaagccga agcgttatta gcagaagcgg gtgtgacaga gttaaaattt gaattgttgg
     6901 caaccgatca ctcttgggtg aaagaatgtg cgccattaat tcttgaatcg tggaacagct
     6961 taaaaggcgt gaaagtttca ctcaaacact tacaatcagg tgcattatat ggcacacacg
     7021 ttgataaagg tgcatacgaa gttgtgattg cacctggcga cccatcagtt ttcggtaatg
     7081 acttagacct attattaagc tggtggtatc gtggtgatgt atggccgaaa cgtcgtttcc
     7141 gctggtctga aacagctgaa tatgcagaag tacaaaaact gcttgatgaa gcggccaaaa
     7201 atcctgctgc ttctaaagaa gcctgggcga aagcaatcaa tattatcgct gaacaagtgc
     7261 ctctttaccc aattattcac cgtaaattgc caactgcgtg gaatgataag gcattaacag
     7321 gtttccaacc attaccaaca acaggtatgt catttatcgg tgttggtcgt gctaaatagt
     7381 cgtaacggct agctaaatgc aagcggtaat attttacaaa aaatttaccg cttgcataaa
     7441 taaaaatcaa aatgatgtaa tattcgtctt atatcactca gaattcttac catttctata
     7501 ttttcattta taatcctaca tatcactata ttagcttgta ggtaaactta gatttaattt
     7561 tggagtgtaa ctacactcaa ggagaagaaa atggaaatta tactccgatt attatggcgt
     7621 cgcctaatgg ctctgccggt aatgatctta ggcgtaactg ccttggtatt tattgtttta
     7681 caattcaccc ccggagatcc ggcaaccgtt gctctcggtg aaagcgccag cgaagccgct
     7741 aaagaaatct atcgtgaaca acatgggtta aatgatccat tgcttgtaca atattttcgc
     7801 tttttaggga atttattcgt attagatttc ggtatgacaa cccctccaga acagcctatt
     7861 gttgatatga ttgctaaagc attcccttta acattacagc ttacctttat tggggtcttc
     7921 ttagctgcta tcgtctcttt cacattaggt gtaacggcag cactttaccg tgatacttgg
     7981 attgatcaat taatccgttt aatctcggtt gccgctgttg ccacaccttc tttctggttg
     8041 ggtattttat tgattcaata cttctcctta aaattagatt ggttgccttc aggcggtttt
     8101 attccattta gcgaagatcc aaaaggctat gtaaactcaa tggttctccc ttcactcgct
     8161 ttagctgttc ctgtatgtgc ctcattgatc cgtgtcgtgc gtacaacaat ggtagaagaa
     8221 atggataaag attatgttcg aacagccatc ggtaatggtg taccttactc aacggtgatt
     8281 cgccacaacg tgcttcgtaa tgccttaatt actccggtaa cagtattagg tttacgggtt
     8341 ggttatttat taggtggtgc agttgtgatt gaacagatct tcgacttacc gggaatgggt
     8401 aaattaattt tcaacggtat cgtcaatcac gacttgaact tagtgcaagg tgtagtatta
     8461 actatcgcat ttactttcgt gttggtgaat atcattgttg atattcttta cttgctcatt
     8521 aatccaaaaa ttcggagttt ataatatgtt tcgccaagga ttagctgctc gacttgcttc
     8581 aggcggtgca agatttagag cattatcaac cagctcaaaa atcgccttac tctttattgt
     8641 atttatcgca ttagtcgcta tttttgcacc attagtagcg ccttatgacc cactgcaaac
     8701 gctacgtcca gtgcaagctc caagcggtga ttatttattc ggtacagacc gtttaggacg
     8761 tgatattttc tcgcgtatgg tatggggagc aagaacatct ctctttatcg gtttaggtgc
     8821 ggtaggcgtt gcgattctat tcggtagtat tttaggggca actgcggcaa ccgccgataa
     8881 attcggtaac gaagtgatta tgcgttttat ggatatctta atggcattcc cgggtatcgc
     8941 attagctgct gtattacttg ccactttcgg taactctgta cctgtgatta tcgtgactat
     9001 tgcggttgtt tacactcccc aacttgcccg agtggtgcgt gctaacgtag tatctcaatg
     9061 ggaagaagat tatgtgcgtg ccgagcgtgt aattggtggt agccgtactt atattctatt
     9121 aaaacacgtt gtgcgtaata ctgctgcacc ggttttagta ttcgcaacag tgatggttgc
     9181 tgatgccatc gtatttgaag catcactttc attcttaggt gcaggtgtcc agcctccatt
     9241 cccatcttgg ggaaatatct tatctgaagg tcgtaacctt gtattaagtg gcttctggtg
     9301 ggctacaact ttcgcaggga ttatgatttt attaaccgta cttgcgttaa atatccttgc
     9361 tgaaggctta accgatgcgt tagtaaatcc aaaactaaaa cgttcaactc aaagtaaaga
     9421 agatgtcgca aaaccattgg caacagacgt tcaagaagca atgtctgaaa ctcttgcgtt
     9481 aaaacgttac ttactcaaat tacacgagaa agaaacgacc cgtactgacc gtatgcaatt
     9541 aaactcaacg gccaaaccga ttttacaagt gaaaaactta tcaatccgtt tcccaaatcg
     9601 ttatggtgaa actccgcttg ttgataatat cagctttact gttcacgaag gcgaaaccat
     9661 gggcttagtg ggtgaatctg gttgtggtaa atcaatcacc gcattctcaa ttatgggttt
     9721 attaccaaaa actgcaaaaa tcactggtga aatcctcttt accgaccgtt caggtaaaca
     9781 acacaatctt ttagaatcag ccgatttaaa tgcccttcgt ggtcacgaaa tctcgatgat
     9841 ttaccaagat gcattaagtg cgttaaaccc atcaatgcgt attaaagacc aaatggcaca
     9901 attaatcagc cgtggtggta aacaatcagc agaaacatta ttgcaatggg taaaacttga
     9961 cccggaaaaa acgctcaacc gttatcctca cgagttatct ggaggacaac gtcagcgtgt
    10021 attaattgca atggcacttg ctcgtgaacc gaaactattg attgctgatg aaccaacaac
    10081 agcattagac gtaaccgttc aagctgaagt gatcaaatta ttaaacgaat tacgtgagaa
    10141 acttggattt gcaatggtat tcgtgagcca cgacttagct ttagtggcac aaatcgctca
    10201 ccacatcacg gtaatgtatg ctggtcaagt ggttgaaact gcaccaacta cgccattatt
    10261 ggcaaaccca acacacgaat acacacgtgg attattaggc tcggtacttt ctaccgaatt
    10321 gcgtgcagaa cgcctatacc aaattccggg cagtgtgcct tcaccatttg attttgcgaa
    10381 aggtgaccgt tttgcgagtc gttcattaag accagatgca aaccctgaac agcacttgaa
    10441 attagtgcca accggtgatc acccacaaca cttctgggct tcacacttag aagatccaca
    10501 atctaaagaa gcataaagga gagcaatacg atgagtatga aatttatcaa agaacagcca
    10561 attattgaac tgtctaatat tgttgtgcaa tttgcttctc gtgacggcac cttatttaac
    10621 ccgaaaaaat tcacggcggt aaatgatgtc agcctagcaa ttcacgccgg tgaaacagtc
    10681 ggtctggttg gacaatctgg ttgtggcaaa tctactttag ccagtgtgat gatcgggctt
    10741 caaaaaccaa cctccggcac agtaaaattt aacggcttag agatgaaata tggcagtgcg
    10801 gaagctcgca aatatttcgg tagccaagtg tcagtaattt tccaagaccc ggcaaccgcc
    10861 ctaaatccgc gtatgcgtgt gttggatatt ttaaaagatc cgatggatat tcataatatc
    10921 ttacaaccgc aagaacgtga gaagcgggtt tatgatttgc tctctcgagt cggtctacct
    10981 cgttcagcgg cgttggtcga gccaacccga ttatctggtg ggcaaaagca acgtgtagcg
    11041 attgctcgtg cattggcatt aaatccgaag ctgattgtgg ctgatgaacc aacctctgca
    11101 cttgacgtat cggttcgagc tcaagtactg aacctgctgg cagacttgaa aaaagaactc
    11161 aatttagcga tggtatttat ctctcacgac ttacaaaccg ttcaccaagt ttcagaccgc
    11221 attgtggtta tgaacggcgg taaaattgtg gaaatgggcg atgcttcaga cgtatttgaa
    11281 cgcccaacgc aagagtacac ccgaacctta attgaagcag caccatcttt attataattg
    11341 tgctttgtgc ctacaagcgg taaaattttt cgcaaatttt accgcttgtg tttttattta
    11401 tgatccaaat tacccaaacc attaacattg atttattaat tgtcggctct ggcatcgctg
    11461 ggctttctgc tgcagttgag gcaaaagaaa aaggcattaa taccttactg attagcaaat
    11521 ctacgattgg ctcgggagcg agctactttc cgcttaaggc aacacttggc attcaagtaa
    11581 ccggcgatga agccgataaa attcacttta aagaagatat tgaaaacatc ggcaaaggca
    11641 aaatcaaccc taatgtggta caagcctata tcgaagattc accacaagcg attgaactac
    11701 tccaccgtat cggctttcag ccttggaagc gtaacgacaa ccgtccggct tgttttgcca
    11761 aataccctcg cccaatttat ttaattaaag attggcggga atctgcccta cgagcgaaac
    11821 agattttgca agagcaacac attgctttct atgaaaatgc taccctttta cacatcgtca
    11881 cagacaataa ccaagtgcaa ggtgcggtat tttcgctcac tacaagcggt caaatttcct
    11941 atattttttg caaaaccaag catcttattt tagccgcagg cgggattgct gggctgtata
    12001 aagataatct ctacccggct gatgttatcg gttcacttca ttatgtggca caacaagccg
    12061 gggcaacctt ggttaatctt gcctttattc agttcattcc tgcctttgtc gagccaaaat
    12121 acaaggtgct atttggcgaa catacattga aatatgtcac ccaaatcacc gattctaagg
    12181 gtaacaatct atttccacac ttaaatgccg agcaatttgc aaaaatggtg ctggaacgta
    12241 gccattatgc tcctttcagc cttgattttg ccggcgtgga gttcgattta gtgatgatga
    12301 aacatttgct tgaaaaccct cacgaaaaag gcatttacct taaatacagc cccaaactgt
    12361 atgaagacac ggaagagttt tataccgtct atttgaattg gctcaaaaat gaagtcggga
    12421 ttgatttagt gcaagataag attgcaatca agccgtttgc tcatagctgt aacggcggca
    12481 ttcaaattaa tcaatggggc gaaagtgcag taaaagggct atttgcgatt ggtgagattt
    12541 ctcactgcat tgaaggggca aaccgtatgg gtggtaactc agtcggcggt ggcttagtgt
    12601 ttgccaaacg tgcggtatcg gaaattgttc gttcgctaga acagaatgat ttgcaaaaaa
    12661 acagcgaaat ctcaccgctt gcaatggcag aaaaagcatt aaatcagctt gctaattcaa
    12721 ctgctaatct agaactaact gccagtgaag tgctttctac tattcgagag aaaatggcgt
    12781 tatacgccaa tgtgtaccgc actcggagca acctcaaact actacttgag gaattaaacc
    12841 gtttggaaca agaatttgac ccaatctatc acgctcaata tcaaggcatt gaaatttaca
    12901 atgcgttaaa aaccgctcaa ttagtggtca atcaaatgct aaatgagcag agtttaggcg
    12961 cgcactactt agatgaacaa aattagtcta taatagccaa cttatttaaa aataataaat
    13021 taggagtata aatgaataac tacctactta aattaaaacc aatgacagcg gtcatacttt
    13081 cgcttttgac ggtctttatc ttaggtttta caatgattgg gcttggctgg gtgcctcaaa
    13141 tttcaattct agccgtgatt gtaatgctgc tacttttcgg cttaatcaat aaaattccgt
    13201 ttaaaaatat gcaagagggt atggcaagcg ggctgatgtc cagtattggt gcggtttacc
    13261 tattcttttt tattggatta ttggtttcag ctttgatgat gtctggtgcg attccaacct
    13321 taatgtatta tggcttaagt gtgatttcac ctgaattatt ttacatttct gcattcgcat
    13381 taagttctgt agtaggtatt gcgttaggca gtagtttaac cacaaccgcc accatcggtg
    13441 ttgcttttat cggtatggct tctgctttcg gtgcgaaccc tgcgattgta gcaggtgcgg
    13501 tggtttcagg ggcgtttttt ggcgataaaa cctctccact ttcagacact actaccattg
    13561 ctgcttccgt agtgggcatt gatatgttcg ctcatatccg taatatgctt tataccacca
    13621 tccctgcgtg gattttaaca gcattattcc tttggttttt aaccgaacct actgatgcac
    13681 aaaatttagc aaatatagag aacctaaaaa ccaatttgca agaaacagga ttaatacacg
    13741 gctactcttt gctacctttt gttacgttaa ttttaatggc aattaagcgt gttaattcaa
    13801 tttatattat tgtaactaca gtaacactct cgctaattat tacctatatc cacagttcgc
    13861 caacccttga acagctaggg aattatttct ttaccagcta taaacctgtt gaaggcgtag
    13921 atttaggcga agtggcatca ttagtctctc gtggtggcat taatagtatg ttctttacca
    13981 tcactattgt attgcttgcg ttaagtttag gtggtctttt acaaatcctc ggcattttac
    14041 cggcactact taacggcatt agcggctttt taaccagcac aggacgtgtt actcttgtag
    14101 ttgccagcac tgcggtggga attaacgtct taatcggtga gcaatatttg agcttattgt
    14161 tatcaggtaa tactttccgc tcaacttatg aacgtttagg cttagaagtg aaaaacttat
    14221 ctcgcaccat tgaagatgca ggcacggtaa tcaacccatt agtcccttgg ggcgtgtgcg
    14281 gtgtgtttat cagtagcgta ttaaatgtac cggtgttaga ttatctacca tacgcattct
    14341 tctgttatat ctgtttaatc cttaccttaa tttttggatt taccggggta acaatcagta
    14401 aaaaagctaa ctaatatgtg aaaaatgcct acaaatttgt aggcattttc ttttcattag
    14461 agctgaagaa cagcttgttt ggctaactct ctggcgagtt tatcaatatg caatacatca
    14521 gcaatttcac tgacttgaat tggagcaata ttttccacca ccgttctatt cacgttcaca
    14581 atatccgtaa agcggatttg ctcgtttaaa aacgcctcaa cggcaatttc attggcggca
    14641 ttcatcgctg ttgtcgcata ctgtccttct gcaaaagcct caatcgctaa ttttaagttc
    14701 gggtaacggg caaaatccgg ctcgataaag gttagctctt ttaatttaaa gaaatctaat
    14761 ggtggcacac cagcattaat tcggtttgga tatgccatcg tgtgagcaat cggcgtacac
    14821 atatccgggt tgcccatttg agcaatcacc gagccatcaa tataacgcac catcgaatga
    14881 ataattgatt gtgggtgaat gatgatatcc atttcctcgg ctgttgcatt aaacaaccag
    14941 cgggcttcaa tatactccaa gcctttgttc atcattgtgg ctgaatcgac tgaaattttc
    15001 ttacccatag accaattggg gtgagcaact gcttgagccg gtgtaatcgc atcaaattca
    15061 tttaatggct tagtacggaa cggtccaccc gatcctgtta aaataatttt actgacaccc
    15121 agttcagcga gtggacaaaa cccgactttt tgctgtgcct ccggtggaag cgattggaaa
    15181 atcgcattgt gttcactatc taccggtaag agcttcgcac cggatttacg agcctcatca
    15241 ataaaaattt gcccacaggt taccagcgat tctttatttg ccaacaatac ggttttaccc
    15301 gctttcaccg cagaaagtgt cggtaataac cctgctgctc ctacaatagc agccattacc
    15361 atatccactt cggaatgtgc agcaagctca caaatagctg cttgaccggc taacacttca
    15421 gttttagacc ccaaagttgc caatgccgtt tttaatgctt gtgcggattt ttcatcatcc
    15481 attgccgcaa aagtcggctg atattgacga cattgctcca gcattaattc aacattcttt
    15541 ccaccaacta acgcaaacac ctcatattgg tttgggtttg ggttatgctc aactaccgat
    15601 aaagtgcttt taccgataga acctgttgaa cctaaaatca ctaatttttt cattttgttt
    15661 ctctttatct aatgactgat aagggcgaaa gatttttcgc ccctacgccg taattatgcg
    15721 gatactcatt cgttgtttgt aaattgttta gcaaaaacaa ccgcttgtag attaactcga
    15781 aagccgtttt gcttcgataa catcatctaa ttgagcgata cgagccaata atttattcaa
    15841 aatttcaacg ttgttaagct gaatttccat atcaatggtg gcaaggctac gtttcacatc
    15901 ggttcggctg gaaacgccta acacgtttac tttttcattt gccataattg cactgacatc
    15961 tcgcagtaag ccgttgcggt cattggcaat cactcgaatg gttaagctta aaccagaggt
    16021 atagccttcg ccccaatccg cctccacaac tcgttctggg ttgagacttt gtaattcaaa
    16081 cagttgctca caatctgcac ggtgaatcga aatccctctg ccttgtgtga tgtaacccac
    16141 gatttcatca cccggaatcg gctggcagca acgggcaatg gaatgcatta agttccctac
    16201 gccctctaca atcacatagc ctttgcttgg cttcggctga ttggtgtttt tattcacctg
    16261 tttaagtacc gcttcgtctt cttgttcggc ggtcggttta attaattttg attgcaagaa
    16321 gtgcattaac tgatgaagac gaatatcgcc accgccaata ccggcataga gatcatcaaa
    16381 ctgttttaag ttatagcgag gcagagcata ttcttcaatt tgcttgagcg taaaaccgaa
    16441 tttttccatt tcggcttcaa gggcctcttt gccgattgga atatttttct ctcggtctaa
    16501 ttttttaaac cacgcaataa tttttgagcg agctttgcct gtattcacaa aaccggtatt
    16561 cggattgagc caatctcggc ttgggttcgg ctctttttgg gtgataattt caacctgatc
    16621 gcccatttgc agatgataag taaacggcac aatccgccct gccacttttg ctccaatgca
    16681 acgatgcccg atttcactat gaattgcata agcaaaatca agtggtgtag ctttttgcgg
    16741 taaatcgatc acctcgcctc ttggggtaaa gacataaaca cgatcatcaa aaatttgcga
    16801 acgcatttct gccaccatat cgcctgaatc ggcaagatcg ttttgccact caagcaattt
    16861 gcgtagccac acgatttttt cttcgtagcc cgaacgccct gcggttgccc cctctttata
    16921 tttccagtga gcggctacgc ctagctccgc atcatcgtgc atttgctggg tacgaatttg
    16981 cacttcaatc gttttttcac cttcgcctaa tacaactgta tgaatagact gataaccgtt
    17041 aggttttggg ttagcaatat aatcatcgaa atgttccggc aaatgggtat atttggtgtg
    17101 aataatgcct aatgccgtgt agcaatcttc taacgttggc actattaccc gcacagcccg
    17161 aatatcgaaa agctgattaa atctcaaccg ttttttctgc attttcttcc aaatgctgta
    17221 gatatgtttc gggcgtccat aaatctgcac attcttgatt tcaggactta aacaagcggt
    17281 cagatctgct acaaaatttg caatataacg ctcacgatcc acacggcgtt cgcccaattc
    17341 aaatttagca atttgagtgt aagagacagg gtgtaaaatg cggaaacagt aatcttccag
    17401 ctcccatttg agctgcccga tacccaatcg attggcaagc ggtgcataaa cgtgagaaca
    17461 ttctttagcg gcgagaacgg tatcttcttc ggaatggtgg ttactatcac gcaggtaggc
    17521 aattcgttcg gcaagtttaa tcaccacaca gcggaaatcg tccaccatcg ccaacaacat
    17581 gcgacggata ttgtcgattt gcagatccga cacattattg gcactaagct ggcggatatt
    17641 ctccatttcg agtacgcctt taactaagtt tttgatgtcg ttactgaaat cttcttttaa
    17701 ttgcaccaag tcgatcacat tgtgttttac cagaggaaaa agcattgcgg caactaagct
    17761 gtcatcgtcc atattcaagc cgtgcaatac ttcaaccatc tcaatgccat accacatcag
    17821 actatagtcg gtatccagtt tttcctgtgc ataactccac gcattgtgaa tctgctcaaa
    17881 agtcacagga gacatattca ggcttgcaca ccaactcgac aactcaaaat tattcggttc
    17941 aagttgatgg gaataacgaa ttgctaccat aaggcctcct tgtaaatttt tgtggaaatt
    18001 agaccgcttg tacagcttaa aatggagttt tattatacgt ggaaattcag aaaaaattta
    18061 cccctaatcg aaaatttaat ccaagttatc ttatcctctt tactccttaa gaggtagcaa
    18121 ccttgtgata aatcaaacct atcaacacaa tcatttgcac gacttcacgc tcttgattaa
    18181 aatacttgcg tttttatttt gatctttatt tttgtgggta gcaatgacaa ttttagcttt
    18241 aggaatcaac cataaaaccg cctctgtggg tttaagagag aaagtggcgt ttgtagatga
    18301 caaacgtaaa ctggctcttg aacaaatcca aacgagcgga ttggctgaaa gcgtggtcat
    18361 tctctctacc tgcaaccgca ccgagctcta cttccaccag cctaatattt ctccccgtga
    18421 agagagcgag gaaaatatcc aatggcgtga acaatgtttc cgctggtttg cccaaattca
    18481 tcaacttgat gaaagcgagt tgcgtgaatg tttatatttt aaacaaaatt tagaagccgc
    18541 taaccactta atgaaagtgg caagcggatt agattcacta attttaggcg aaccacaaat
    18601 tttagggcag gttaaacaag cctatcaata tagcgaagat ttctaccaac atcagcaaaa
    18661 agcgatgtcc accaagctat cacgcttgtt ccaacgcacc tttgccaccg caaaacgagt
    18721 acgttccgaa accgaaatcg gcagccaagc ggtgtcagtt gcttatgcag cttgcggttt
    18781 agcccgtcaa attttcgata attttggcaa gttacgcttt ttgctggtag gggcaggcga
    18841 aaccattgaa ttagtcgccc gctatttaat tcaacacggg gcgaaaaata taatgttggc
    18901 aaaccgcacc catattcggg cggaaatgtt ggcggaaaaa ctaaacgctc caatgcaaat
    18961 tctgtcgtta agtgctttgc aactcggttt aaaccaagct gatgtagtta ttagctccac
    19021 cggtagccct gataccctaa tcagtaaaga aatggtggaa attgcgcaaa aacaacgcca
    19081 atttgaccca cttttgctga ttgatattgc cgttcctcgt gatattgacg aaaaagccgg
    19141 cgacttagag ggcgtttacg cttatagtgt tgatgattta caaaatatta tccaacgcaa
    19201 tatggctcag cgtgaagaag ccgccaaaca agccgctgaa atcgtcaaag aggaaagcaa
    19261 agcatttttt gaatggttaa aacaacagca gtctagtgat ttaatcaaac gctatcgtca
    19321 agatgcggag caaacccgcc aagaattgtt ggagaaagcc ctacaagcaa tcagtcaagg
    19381 gcaagacagc caaaaagtat taagtgagct aagctacaaa ctcaccaacc agttactaca
    19441 cgccccaacc caagcgttac aagcaatggc aaaaagtggc aactccaccg gcttacaaag
    19501 tttttcaaga gcattacggc tagaagaatg atgctcagca cattctttta ggcatatttg
    19561 caaaaaattg aacattttta accgcttgcg gacgcacgcc gtgcgtccct ccccgtgttc
    19621 ccgatcttct tctaatttca acttccccaa atttccgcta taataagcca atttttgcaa
    19681 ctattttgca aataaccgga tatagaggat acaaaatgac tcctgttgtt gccctagtgg
    19741 gcagacctaa tgtcggcaaa tcgactttat ttaaccgttt aacccgtact cgagatgcct
    19801 tagtagcaga tttccccggc ttaacccgag accgtaaata tggtcacgct aatattgccg
    19861 gctacgattt cattgtaatc gacaccggtg gtattgatgg tacagaggaa ggcgtggaag
    19921 aaaaaatggc ggaacaatct ttattggcga ttgaagaagc cgatgtggtg cttttcttgg
    19981 ttgatgcccg agccggatta ttacctgccg atgtcggcat tgctcaatat ttacgccaac
    20041 gtgaaaaaac cacggtggtg gttgccaata aaaccgatgg tattgatgcc gactctcact
    20101 gtgccgaatt ctatcaatta ggcttaggcg aagtagaaca aattgctgca gcacaagggc
    20161 gtggggttac gcagcttatc gagcaagtgt tagctccttt aggcgaacag ctaaatgccg
    20221 agcaatttaa ccaagaacaa gcgggcgaaa atgctaaaaa tgttgcaaat gaagaaacag
    20281 acgaatggga taccgaattc gactttgaga acgaagaaga tgcggcatta ttagatgaag
    20341 ccttggcaga agaagcagaa gagtcgattg aagataaaaa tatcaaaatt gcgattgtcg
    20401 gtcgtccgaa tgttggtaaa tcaacactga ccaaccgcat tttaggcgaa gagcgtgtgg
    20461 tagtttatga tatgccagga acaacccgtg attctattta tattccaatg gagcgtgacg
    20521 gtcagcaata taccatcatt gataccgccg gtgtgcgtaa acgtggcaaa gtgaatttag
    20581 cggttgagaa attctcggta attaaaacct tgcaagccat tcaagatgca aacgtggtgc
    20641 tattaactat tgaggcccga gaaggtattt ccgatcagga tttatcacta ctcggcttta
    20701 ttctaaatgc tggtcgttca ttggtgattg tggtaaacaa atgggacggc ttgtcgcaag
    20761 atattaaaga tcaagtgaaa tcagagttag atcgccgttt agattttatt gattttgcca
    20821 gagtgcattt tatctcggca ttgcacggct caggcgtggg gaatttgttt gattcagtaa
    20881 aagaagccta cgcttgtgca acgcaaaaaa cctcgacctc aatgctgact cgcattttgc
    20941 gtatggctgc tgatgaacac caaccaccat tggttaatgg tcgccgagtg aaattaaaat
    21001 atgctcaccc gggtggctac aacccaccaa ttattgtgat tcacggcaat caggttgaaa
    21061 aattagcgga ttcttacaaa cgttatttaa gtaattattt ccgtaaaagt ttaaaaatta
    21121 tcggctcgcc aattcggatt ttattccaag aaggcaataa tccttttgcc ggtaagaaaa
    21181 acaaactgac accaagccaa ttacgcaaac gtaaacgttt gatgaagttt att
//
LOCUS       DANLUQ010000024        20720 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.024, whole genome shotgun sequence.
ACCESSION   DANLUQ010000024 DANLUQ010000000
VERSION     DANLUQ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 20720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20720
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.024"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            32..769
                     /locus_tag="PXB27_000746"
     CDS             32..769
                     /locus_tag="PXB27_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009020527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDL5345565.1"
                     /translation="MRGGKQLAKEQYDVVIDPTVTLRNRDLLFLRTISAKHYVGYQKQ
                     KYRLFDLNVEDRKQHFSEIYQDALALIGFENIETQYDVPLNATSNQSIKHYLYENQIR
                     NYIALNLFGAGSARRFSEQKINELLSCLKQHSDKPIVLLTFPEVTAKLCSFTKKYKNI
                     FVYEKTKSVFDSIELIRFADLLISPDTATVHIASGLSKKIIAFYSSDEQNFIHWHPNN
                     QAETHILRFRKSVNDLDFAAIKPEWLK"
     gene            771..1661
                     /gene="ilvY"
                     /locus_tag="PXB27_000747"
     CDS             771..1661
                     /gene="ilvY"
                     /locus_tag="PXB27_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HDL5345566.1"
                     /translation="MDFQSLKLFLDIAQSRSFIRSAEKNYMTASTLTRHIQRIEEELG
                     QPLFLRDNRQVRLTEAGERFLAFAQQGWAEWQQLQNQLSPAQGDLEGELKVFCSVTAS
                     YSHLPPILGKFRQKYPKVDIKLSTGDPALALEQVQSLAADISVAGKPEFLPNNIVFHY
                     IDDIQLSIIAPRVASQATQFLQQSPIDWKNIPFILPVNGPVRKRIDRWFKEQKIKDPI
                     IYATVSGHEAIVPMVALGFGVALLPDIVIKHSPMNNQVSYVNLPSPITPFELGICVQQ
                     KRLQEPIINAFWQLLSDNSA"
     gene            complement(1795..4197)
                     /gene="lon"
                     /locus_tag="PXB27_000748"
     CDS             complement(1795..4197)
                     /gene="lon"
                     /locus_tag="PXB27_000748"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HDL5345567.1"
                     /translation="MAPRKKKAIELPLLPLRDVVVFPYMVMPLFVGREKSIQALRSAM
                     DSNKQLFLVTQQDPNKEEPNAEDMYGVGVIANIIQMLNLPDGTVKVLVEGQTRAKIEQ
                     IHDDENGFWAAIQPIYSEYDDENEELKAIAKTTLTEFENYVKNNKKIPAEIIAKLQKI
                     TLEDRLADTIASNLIAPVKKKQELLEQPNLIARFEALLIAMATEMDTLETETRIRNRV
                     KQQMEKNQRDYYLNEQIKAIQKELGNGDDVEQSELDKLKEKIDAAKLPTEVKDKLNTE
                     FKKLKAMPQSSSEATVVRGYIDWILQMPWHKKSAVKKDLAQAQEILDKDHYGLERVKE
                     RIVEYLAVQSRLNKLKGPILCLVGPPGVGKTSLGQSIANATGRKYVRMALGGVRDEAE
                     IRGHRRTYIGSMPGSLMMKMAKVGVRNPLFLLDEIDKMAQDMRGDPASALLEVLDPEQ
                     NKAFNDHYLEVDYDLSDVMFVATSNSMNIPPALLDRMEVIRLSGYTEDEKMHIARGHL
                     LAKQQENNGLKEGELTIEDSAILSIIRYYTREAGVRSLEREIAKICRKAVKALVLDKK
                     LKSITVSEKNIEEYLGVKRFDYGKMDSQNRIGEVTGLAWTEVGGDLLTIETASVAGKG
                     KFSYTGSLGDVMKESIQAAMVVVRSRAEKLGIAEDFHEKRDIHVHVPDGATPKDGPSA
                     GIAMCTALISSLTGNPVRKEVAMTGEISLRGKVLPIGGLKEKLLAAHRGGITTVIIPK
                     ENEKDLEEIPENAKAALDIHAVETIDEVLALALENPPMGIEILSKAEIKVKKSRAKAT
                     VQ"
     regulatory      4401..4519
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            4695..5792
                     /gene="ribD"
                     /locus_tag="PXB27_000749"
     CDS             4695..5792
                     /gene="ribD"
                     /locus_tag="PXB27_000749"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005708727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDL5345568.1"
                     /translation="MTDVQYMAYAITLAEKARGWTSPNPLVGCVIVKNGNIIAEGYHQ
                     KAGSWHAERNAILNCEEDLTGATAYVTLEPCCHHGRTPPCSELLIERGIKKVFIGSRD
                     PNPLVSGKGAAQLRAAGIEVVEDFMRTECDALNPIFFHYIQTKRPYVLLKYGMTADGK
                     IATYTGNSKWITGEAARANVQKSRHEYSAIMVGVETVLADDPMLNSRMENAKQPVRIV
                     CDSQLRTPLDSQLVQTARQYRTIIATLNNDAKKQAEYTACGVEILVTGERDKRVDLAD
                     LLQKLGELGIDSLLIEGGSQLNFSVLEQGLVNRVHCYIAPKLIGGKAAKTPIGGKGIG
                     ELAQAVKLRLKSTELLGEDILLDYEVDLLRN"
     gene            6009..6656
                     /gene="ribE"
                     /locus_tag="PXB27_000750"
     CDS             6009..6656
                     /gene="ribE"
                     /locus_tag="PXB27_000750"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDL5345569.1"
                     /translation="MFTGIIEEVGQIAQIKKQGEFVVVTVKAKKILTDVHLGDSIAVN
                     GVCLTVTSFTQEQFTADVMSETLKRTSLGELSINSSVNLERAMAANGRFGGHIVSGHI
                     DGTGVVAEITPADNSTWYRIKAEPKLMRYIIEKGSITIDGISLTVVDVDAESFRVSII
                     PHTIKETNLGTKKIGSLVNLENDIVGKYIEQFLLKKEPENPQSKITADFLKNAGF"
     gene            6675..7874
                     /locus_tag="PXB27_000751"
     CDS             6675..7874
                     /locus_tag="PXB27_000751"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HDL5345570.1"
                     /translation="MFKFSTVEEAIQAIAQGKIILVSDDADRENEGDFICAAEFATPE
                     NINFMAKYGKGLICTPISTEIAKKLNFHPMVLENEDNHQTAFTVSVDHIQTGTGISAF
                     ERSLTCLKMLDENAKPEDFRRPGHVFPLVAKDGGVLVRNGHTEATVDLARLAGLKPAG
                     LCCEIMAEDGTMMQMPELQAFAQAHNMPFITIQQLQEYRRKHDSLVNVVSVVKMPTKY
                     GEFKAHSFVETISGKEHVALVKGDIGDGENVLCRIHSECLTGDAFGSQRCDCGQQFAA
                     AMNQVEQEGRGVVLYLRQEGRGIGLINKLRAYELQDRGMDTVEANLALGFKDDEREYY
                     IAAQMFEKLGVKSIRLLTNNPAKIEGLIEQGLNVVAREPIMVEPNEHDLEYLKVKQHK
                     MRHMFNF"
     gene            8080..8544
                     /locus_tag="PXB27_000752"
     CDS             8080..8544
                     /locus_tag="PXB27_000752"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDL5345571.1"
                     /translation="MATFQGNYIATGLKFGIVATNWHKIFIDQLLNGATDKLLRHGVE
                     EKNIDTVWVPGALEISIAAKKMAQSGRYDAIICLGAVVRGATSHYDVVVNESAKGISS
                     SALETGVPIINGILTVENLEQAIERSGTKAGNKGEECAMVAIEMANLLKALA"
     gene            complement(8590..10098)
                     /gene="lnt"
                     /locus_tag="PXB27_000753"
     CDS             complement(8590..10098)
                     /gene="lnt"
                     /locus_tag="PXB27_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HDL5345572.1"
                     /translation="MNFAKNPPFLTACLLAIILGAIGTFAYSPFDIWILAFASAAGLV
                     WSATLANRKTALWATFAWSIGYFCTGVNWVSVSMTQFGGVPTIVSYIAVFLLACYLAI
                     YNLLFSVVSHRLKLNNPFALAAIFTFTEYLRGVVFTGFPWLQFGYSLIDSPFAGIAPI
                     LGVEGLTFLVIVASSYLVKIIQQENKTVPSLMVLAVIFGLSFATRFLQFVQIDTEKQP
                     LVVSLVQGNIEQKMKWDPQHFNHSVRTYEQLILPLLGNSELIILPESAIPALENQISP
                     LLGSLDHLARQHGSEIIIGTLYQNEQEELFNSSVLLGEKEREYALHSNPRYNKHHLVP
                     FGEYVPFGSVLDWMREVFILPINLSQGSFVQPSFLAKNNRFNMAICYEIIFGDQLQQN
                     QKSQNSDYLLTITNDAWFGSSIGPWQHFQMARMRALELGKPLLRAANTGITAIVDANG
                     KVIEQLPQFTADALTATIVPTKGDTPFKQLGHWLIYSFSLFCVIASLLLRRK"
     gene            complement(10157..11056)
                     /gene="corC"
                     /locus_tag="PXB27_000754"
     CDS             complement(10157..11056)
                     /gene="corC"
                     /locus_tag="PXB27_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249295.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HDL5345573.1"
                     /translation="MSDEQQSVNTTEKSEKKSILHSLFSGLFQNEPKNREDLVEVIRD
                     SVENELIDSDTKEMIEGVMEISELRVRDIMIPRAQIVFIDANEPLDVCIDLIIESAHS
                     RFPVIRGEKDTIEGILHSKDLLKYLRSDSEPFNLQQVLRPAVIVPESKRLDRMLKEFR
                     SERFHMAVVVDEFGAVSGLVTIEDILEQIVGDIEDEFDEEEVKPIRQLSRHTYAVLPL
                     TDIKKFNEFFATDFNDEEVDTIGGLVMQAFGYFPKRDEQIELNGIDFKVTSANSRQLI
                     QLRVTLSDEQLEKMEKTSPNEEE"
     gene            11194..11802
                     /locus_tag="PXB27_000755"
     CDS             11194..11802
                     /locus_tag="PXB27_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HDL5345574.1"
                     /translation="MKLTKIALSLTALVVSSMSVAGTLSTSSMVDILAFDGQRVKSGT
                     TSVQMSENKTHQVVVEVGGTVEGDFFTSDQIILTFQGSAENAKLETPRLNSKLDLRKF
                     QENQTVAIKTDSGKVISHKQDFLKGEGFLAKTRVEDNLTKYNLAKKTASVEQFATAPL
                     EAKGQIVVDTNKVSQEELQLLFRKADKDTQKRFLEWAKKNIK"
     gene            complement(11883..12266)
                     /locus_tag="PXB27_000756"
     CDS             complement(11883..12266)
                     /locus_tag="PXB27_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345575.1"
                     /translation="MKTLKLGCIFSLALMLSSCTLTPEQQAERKAKQIRAEQDLQVQL
                     AKQCDVETAELMYQQFNPPLSQTEQEEKAFKKRYAEKVNAPMFQACYKLAWQNYKAQV
                     ELEEIRWNYDRDYMYRGWRYCYYCW"
     gene            complement(12296..12865)
                     /locus_tag="PXB27_000757"
     CDS             complement(12296..12865)
                     /locus_tag="PXB27_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDL5345576.1"
                     /translation="MNETTINDFSLLCRLFGNLFYRHPTDPILAGTFNWLAQGGLRQV
                     WALNTDSQSENALTLLQKNANPTQLAASYQALFAENGAISPTISAYGLSVNNFVAFRQ
                     ERAMPELENADHIGLLFLTASWIEDNLDSALAQKILFEQFLLPCAGKFLGKIEAFDNG
                     FYKALAQLSRDALSAMADELEESELEAVE"
     gene            12924..13769
                     /gene="nfo"
                     /locus_tag="PXB27_000758"
     CDS             12924..13769
                     /gene="nfo"
                     /locus_tag="PXB27_000758"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDL5345577.1"
                     /translation="MKYIGAHVSASGGVENAVLRSVEIGANAFALFTKNQRQWQAPAL
                     KAETIEKFKRFCKVHHFSAEQILPHDSYLINLGNPEAEALEKSRVAFIDEMSRANQLG
                     LKLLNFHPGAHLKQISEQDCLARIAESINIAVEKVPNVIAVIENTAGQGSNLGWHFDH
                     LATIIEQVEDKSRVGVCLDTCHLFSAGYDISSYEKSEAVFNDFNQTVGFHYLRGMHLN
                     GSKTPLGSRVDRHHTLREGTIGTEVFAFIMQNMHFNHIPMILETISPEIWADEIKFLR
                     TLELK"
     gene            13897..14469
                     /locus_tag="PXB27_000759"
     CDS             13897..14469
                     /locus_tag="PXB27_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL5345578.1"
                     /translation="MKKNLFATTFKPVFIASILSVFGGYSSIAVASLSSKPATPNQII
                     ASKAERLAKSSSVFTNRANLPTSSAHTANYSGRINSVYHNWVGTRYRLGGTTKAGIDC
                     SAFVREVMGKAFSKDMPRSTAEQRHVGHSISKSDLRPGDLVFFRKNNHVGVYIGNGKF
                     VHASSSRGVTTSSLSESYWSRNYTQSRRVI"
     gene            complement(14536..14994)
                     /locus_tag="PXB27_000760"
     CDS             complement(14536..14994)
                     /locus_tag="PXB27_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL5345579.1"
                     /translation="MASIRQISDQTAAYQPIPLYRDFNSSRSVKLDLNSLCIKRPRDT
                     FFIHVKNPNLIAWGIELDDLLIVEQTNQYLVNDLLVLEKDGEYKFYQFFNEIQNGEGI
                     AEKILFSLDVSESNLRITDWAEVKIAGVITNVVHQMRTRGAFTQAKQRVA"
     gene            complement(15168..15551)
                     /gene="grcA"
                     /locus_tag="PXB27_000761"
     CDS             complement(15168..15551)
                     /gene="grcA"
                     /locus_tag="PXB27_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HDL5345580.1"
                     /translation="MIKGVQITESSNSNLLNSFWLLDEAKNEARCLAAKAGFEEDQVV
                     AISELGDIAYREVPVNVAPTIKVEGGQHLNVNVLRRETLEDAIQNPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVITRTFTESL"
     gene            15710..16399
                     /gene="ung"
                     /locus_tag="PXB27_000762"
     CDS             15710..16399
                     /gene="ung"
                     /locus_tag="PXB27_000762"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDL5345581.1"
                     /translation="MKTWTEALGEEKQKAYFQQILQYVHNERLAGKVIYPPQNEVFSA
                     FALTEFSDVKVVILGQDPYHGPNQAHGLAFSVKPPVAPPPSLVNMYKELSEDIEGFQI
                     PPHGYLIDWAKQGVFMLNTSLTVEQGRAGSHSQIGWEIFTDKVIAQLNQHRDNLVFLL
                     WGSHAQRKGQFIDRNRHCVLASPHPSPLSAYRGFFGCKHFSKANNYLISQGISPINWQ
                     LSVDVSYSSTH"
     gene            16415..17335
                     /locus_tag="PXB27_000763"
     CDS             16415..17335
                     /locus_tag="PXB27_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HDL5345582.1"
                     /translation="MNFDLPIVSIAFVVLVLVALSSTIKIVPQGYHWTVERFGRYTKT
                     LSPGLNIVVPFIDRIGRKMNMMEQVLDIPSQEVISRDNASVAIDAVCFVQTVDARRAA
                     YEVNHLEQAIVNLTMTNMRTVLGSMDLDDMLSQRDLINGRLLSIVDEATNIWGVKVTR
                     IEIRDVRPPKELVAAMNAQMKAERNKRADILEAEGIRQAEILRAEGEKQSRILKAEGE
                     RQEAFLQAEARERAAEAEAKATQMVSEAIAKGDTTAINYFIAQKYTEALKEIGSSDNS
                     KVVLMPLEAGNLIGSVAGIAEILKSNKTSS"
     gene            17371..17808
                     /locus_tag="PXB27_000764"
     CDS             17371..17808
                     /locus_tag="PXB27_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDL5345583.1"
                     /translation="MDWFFNWIGWSILGFVLLALELVLPGVFIMWWGFASLILAFIVA
                     LFTLGQALQIALFAIIATIFSLIWWKYQHGKDKKEDQSSSLNSREHAMLGAQGFVVEI
                     LPNGIARGKFGDTTWRVIGNNLQVADKIEVQKVEGITLFVRKQ"
     gene            complement(17949..20585)
                     /locus_tag="PXB27_000765"
     CDS             complement(17949..20585)
                     /locus_tag="PXB27_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDL5345584.1"
                     /translation="MDEGFMVKHPRHSSHLHPLIHQLTTPEGEVRIYDASYPQFEVNP
                     VEKEDGIDLIPSLETHGAGVAGIIAAQADKTLGDGYSGGIAKGAELYVATKSYKRTLE
                     KVIQDAKKELENAKDEEDEKTPSLDQMAKNDLLASKEKEMAIERAEWASGLNKLLDNN
                     VFAINNSWNPFSISDDINVVDKFYQSIKQNKHNPLLQAIMRAKNSNTLLVFAAGNESK
                     KQPGVMALLPRYFPELEKNLISAVAVDKEQKIASYSNHCGASKNWCVAAPGDLHVLIG
                     VADEHKKPQYGLTKEQGTSFSAPAITASLAVLKERFDYLTATQIRDTLLTTATDLGEK
                     GVDNVYGWGLINLKKAVNGPTQFLNDETITVTRDDHWSNPLASQFKITKKGDKSLHLD
                     GENHLDTVAVEEGRLALNGKTKVKTISNHANLAVNGTEVEQNYSSSGQSQLEVLGKSG
                     LIANAQANIHLAGSLKIDDKLTEKTEAGDVSATVVQLKDKATYQGGFTQLVENENLAK
                     RGLIQDLYFKESEIIAKVNKPLTDEKADTNGQAGLALLNALRTTPIAYRRSWYNGWLQ
                     SALEQRKLDNLHYAVSNNIYADSLELLRSQNAKGLTQAQQHLFTAYHTPLQTTVWAEH
                     LNQKQSASSKHTDVKHHQSQLGVNHKLADKTVLSATLSQQKNRLEKPFAQATLKQTAL
                     NIGLRYHLDNAWFSEATLQFARQKYQQSRRFASHQLGTAETRGSTLGGEMRIGYQFMP
                     NQWIIEPSLGVQWIQTKMNGLNESGELATQTAAMRYRDVNIVPSVKLQRTFQLEQGSI
                     SPYIGLNYLHRLNGKITKITSNIAGKTLHSEATTKRNRQLNGEVGVKLHYKNWFTAMN
                     LDYSRVKSANLFGWKVNVGFSF"
BASE COUNT         6066 a         3999 c         4333 g         6322 t
ORIGIN      
        1 tttggttaag acaaaaagta ttcctgatta tattcgtggt ggcaaacaac ttgcaaaaga
       61 gcagtatgat gttgtaatcg atccgactgt tactttacgg aatcgtgatc tcttattttt
      121 acgcactata tcagccaaac attatgtagg ttatcagaaa caaaaatatc gattatttga
      181 tctaaatgta gaggatagaa aacagcattt ttcagagatt tatcaagatg cgttagcttt
      241 aatcgggttt gaaaatatag aaactcagta tgatgtgcca ctaaatgcca caagcaacca
      301 atccataaag cattatttat atgaaaacca aattagaaat tatattgcgt taaatctttt
      361 tggtgcaggt tcggcaagac gttttagtga gcagaaaatt aatgaactgc tgagttgctt
      421 aaaacagcat agtgataaac cgatagtctt acttactttt cctgaagtaa cggcaaaatt
      481 gtgttccttt actaagaagt ataaaaatat atttgtctat gagaaaacca aaagcgtttt
      541 tgattcaata gaactgattc gttttgcgga cttattaatt tcacctgata ctgcaaccgt
      601 gcatattgcc tcaggcttga gtaaaaagat aattgctttt tacagtagcg atgaacagaa
      661 ttttatacat tggcatccga ataatcaggc tgaaacccat attctccgtt ttagaaaaag
      721 cgtgaatgat ttagattttg ctgcaataaa accggagtgg ctaaaataat atggacttcc
      781 aaagccttaa acttttccta gatatagccc aaagccgtag ttttattcgc agtgcggaga
      841 agaattatat gacggcttcc acacttaccc gccatattca gcgtatagag gaggagctcg
      901 ggcagccgtt gtttctgcgt gataatcgac aagtgcgttt aactgaagca ggggaaaggt
      961 ttttggcgtt tgctcaacaa ggttgggcag agtggcagca gttgcaaaac cagttgtcgc
     1021 ctgctcaagg tgatttagaa ggagagttga aagtgttctg ctcggtaacg gcttcttaca
     1081 gccatttacc gccgattttg ggaaagtttc gccaaaaata tccgaaagtg gatattaaac
     1141 tgagtaccgg tgatccggct ttggcattgg aacaggttca gtcattggct gcagatattt
     1201 cggttgcggg taagccggaa tttctgccga ataatattgt gtttcactat attgatgata
     1261 ttcaactttc gattattgcc cctcgtgtgg cgagccaagc cactcaattt ctgcaacagt
     1321 cgccgataga ttggaaaaat atccccttta ttttgccggt gaacggacct gtacgaaaac
     1381 ggattgaccg ttggtttaaa gagcaaaaaa tcaaagaccc gattatttat gctaccgtct
     1441 ccggacacga agcgattgtg ccgatggttg cactaggctt tggggtcgca ctgctaccgg
     1501 atattgtgat taagcatagc ccaatgaata atcaagtttc ttatgtgaat ttaccctcgc
     1561 cgatcactcc gtttgagctg ggaatttgcg ttcagcaaaa acgcttgcaa gagccgatca
     1621 tcaacgcatt ttggcaatta ttgtctgata attcagcata acaagcggtt agattttgct
     1681 aaatttttgc aaaaaataga gcaaaaccga ccgcttgtag ataaaacttt cagataaaaa
     1741 ataaggtgcg tgattgtgat ctgcgcacct tatgttgttt tagattattg cactttattg
     1801 cactgtggct ttagctcgcg attttttcac cttgatctcg gcttttgaca agatttcaat
     1861 ccccattggc gggttttcta gtgccagagc tagtacctcg tcaatggttt ctactgcgtg
     1921 aatgtccaac gctgcttttg cattttccgg aatttcctct aagtcttttt cattttcttt
     1981 cggaataatc accgtcgtaa taccaccacg atgggctgcc agtaatttct cttttaagcc
     2041 gccgattggc aatactttgc cacgcaagct gatttcaccg gtcatcgcta cttctttacg
     2101 cacagggtta cctgttaggc tggaaattaa tgcagtacac attgcaatcc ctgcactcgg
     2161 accatctttc ggtgttgcac cgtccggcac gtgaacgtga atatcccgtt tttcgtggaa
     2221 gtcttcagca atgcctaatt tttctgcacg agagcgtaca accaccattg ccgcttgaat
     2281 agactctttc attacatcgc ctaatgaacc ggtgtaagag aatttgcctt tacctgcaac
     2341 agaagcggtt tcaatggtta ataaatcacc gccaacttct gtccacgcta aaccggtcac
     2401 ttcgccaatg cggttttggc tatccatttt gccgtagtca aaacgtttta cgcctaagta
     2461 ttcttcgata tttttctcag aaactgtaat ggattttaat ttcttatcca atactaaagc
     2521 tttcacggct ttacggcaga ttttcgcaat ttcacgctca aggctacgca cgcctgcttc
     2581 acgggtgtag tagcggataa tacttaaaat tgcactgtct tcaatggtca gctcaccctc
     2641 ttttagtccg ttattttctt gctgtttagc cagtaaatga ccacgagcaa tgtgcatttt
     2701 ctcatcttcg gtatagcctg aaagacgaat cacttccata cggtcgagca atgctggcgg
     2761 aatgttcatt gagtttgagg ttgccacaaa cattacatcg gataaatcgt aatccacctc
     2821 taaataatgg tcgttaaagg ctttattttg ctcaggatct aacacttcaa gcagagcaga
     2881 agccgggtcg ccacgcatat cttgagccat tttgtcgatt tcatctaaca agaacagcgg
     2941 attacgcacg ccgactttcg ccattttcat cattaggcta cccggcattg agccgatgta
     3001 agtacggcga tgcccacgga tttccgcttc atcacgcacg ccacctaacg ccatacgcac
     3061 atatttacgc cctgtggcat tggcaataga ttgccctaat gaggttttac caacccccgg
     3121 cggaccaact aagcacaaaa tcggaccttt taatttattt agacgacttt gcactgctaa
     3181 gtattctaca atccgctctt tcactcgttc taagccgtag tggtctttat ccaaaatctc
     3241 ttgagcttgg gctaaatctt ttttgaccgc tgatttttta tgccacggca tttgtaaaat
     3301 ccagtcaata taaccacgca caacggtagc ttcggaagag ctttgcggca ttgctttgag
     3361 ctttttgaac tccgtattca gcttgtcttt tacctcggtc ggtaattttg cagcgtcaat
     3421 tttctcttta agcttatcta actcgctttg ttcaacgtca tcgccattac ctaactcttt
     3481 ttgaatcgct ttaatttgct cgttgaggta gtaatcacgt tggttttttt ccatttgctg
     3541 tttaacacga ttacgaatcc gagtttcggt ttcaagcgta tccatctcag ttgccatcgc
     3601 aattaacaag gcttcaaaac gggcaatcag atttggttgc tccaataatt cctgtttttt
     3661 cttcaccgga gcaattaagt tagaggcaat ggtatccgcc aagcggtctt ccaacgtgat
     3721 tttttgcagt ttagcaataa tttccgccgg aattttcttg ttatttttaa cgtaattttc
     3781 aaattcggtt aaagtggttt tagcaatcgc ttttaactct tcgttttcgt catcatattc
     3841 cgaatatatt ggctgaattg ccgcccagaa gccgttttca tcatcgtgaa tttgctcgat
     3901 tttggcacgg gtttgtcctt ctaccagcac tttaactgtg ccgtccggca ggtttaacat
     3961 ttgaataatg ttggcaatta cacctacgcc atacatatct tctgcattcg gctcttcttt
     4021 atttggatct tgctgagtga ctaaaaacag ttgtttattg gaatccatcg ccgagcgtag
     4081 ggcttgaatt gatttttcac gtcctacaaa taacggcata accatataag gaaaaaccac
     4141 cacatcacgc aatgggagta gtggtaattc aatcgctttc ttttttcttg gtgccataaa
     4201 tttgctctct ctatcaaaat ataatccccc attatgaggg cgaatttggg aaaaacaagg
     4261 gaaaatttga tttatcttaa aaaaactcga atagtcaaaa cagtagatgt tggcgattta
     4321 atcaattgtt aaaatcaggg taattagctt aatttcccgc ttgacatcaa atcgtaatgc
     4381 gagcaaaatc ccctgcatat tttaatcttc agggcagggt gaaattcccg atcggcggta
     4441 aagtccgcga gccgagcaat cggcaggatt tggtgaaatt ccaaaaccga cagtatagtc
     4501 tggatgaaag aagagtcaaa aaccgcaaaa aataccgcac aagcggtcat tttttgtgtc
     4561 ttttttgcaa gccttgagtc gtgagatttc aaggcttcat tgttttgcag gcggcgtttg
     4621 caaatttttt actaaaaacg accgcttgta ttgcaataat cctttcatta cgccctcaat
     4681 ttatcaaaat gtatatgaca gatgttcaat atatggctta tgccatcacc cttgccgaaa
     4741 aagcacgggg ttggacaagc cctaatccgc tagttggctg tgtgatcgtg aaaaacggca
     4801 acattatcgc cgaagggtat caccaaaaag ccggtagttg gcacgctgaa cgcaatgcga
     4861 ttttaaactg cgaggaagat ctgacggggg caaccgctta tgtaacctta gagccttgtt
     4921 gccaccacgg gcgaaccccg ccttgttccg aactgctgat tgaacgtggg atcaaaaaag
     4981 tgtttatcgg ctctcgagat ccgaacccac ttgtttctgg caaaggagcg gcacagctta
     5041 gagccgccgg cattgaggtt gtggaagact ttatgcgaac ggagtgcgat gcgctgaacc
     5101 cgattttctt ccattacatt caaaccaaac gcccttatgt gttgctgaaa tatgggatga
     5161 cggcagatgg taaaattgcg acttatacgg ggaattcgaa atggatcacc ggcgaggctg
     5221 cccgagctaa tgtacagaaa agccgccacg aatatagtgc gattatggtc ggggtggaaa
     5281 cggtgcttgc cgatgaccca atgctcaaca gccgaatgga aaatgctaaa cagccggtgc
     5341 ggattgtatg tgacagccaa ctgcgtaccc cattggatag ccaactagtc caaactgcac
     5401 gccaatatcg caccattatt gctacgctca ataatgatgc aaaaaagcag gcagaatata
     5461 ccgcttgcgg tgttgaaatt ttagtgacag gcgaacgtga caagcgggtg gatttagccg
     5521 atcttttgca aaagctgggc gaactcggga tcgacagctt attgattgaa ggtggttcgc
     5581 aactcaattt cagcgtgctg gaacaggggt tagtcaatcg agtacattgc tatattgccc
     5641 cgaaactgat tggcggcaaa gcggcaaaaa ccccgattgg tggcaagggg attggcgagt
     5701 tagcacaagc ggttaaatta cgtctaaaat ccaccgaatt gctcggagaa gatattttgt
     5761 tggattatga agtggacttg cttcgtaatt aaattgaaat ttccttactc tcgcccgttt
     5821 acgggagaga gacagcataa aatttcgttc agaaatttta tgcagagaga ggggaaaatg
     5881 agtgtaatct tccctctccc tccgaaaatc ctacggattt tctcttccct ctcccgtaaa
     5941 cgggagaggg gaatagtaag ataagcagtt ctcagactaa attggaagta aatcggagaa
     6001 aaatttaaat gttcacaggc attatcgaag aagttggcca gatagcccaa attaagaaac
     6061 aaggcgagtt tgtggtggta acggttaaag caaaaaagat tctgaccgat gtacatttag
     6121 gggatagcat tgcggtaaat ggcgtgtgtt taaccgtgac ttcatttacg caggagcaat
     6181 ttactgctga tgttatgtca gaaaccttaa aacgtacatc acttggcgaa ttaagtatca
     6241 acagctcggt taatcttgag cgggcaatgg cggcaaacgg gcgttttggt gggcatattg
     6301 tgtcaggtca tattgatggc acgggtgtgg ttgcagagat tacaccagca gataattcaa
     6361 cttggtatcg cattaaagcc gaacctaagt taatgcgtta catcattgaa aaaggctcaa
     6421 ttactattga tggcattagt ttaacggtgg tggatgttga tgcggaatct ttccgggtat
     6481 cgattattcc gcataccatt aaggaaacca acctcggtac gaaaaaaatc ggtagtttgg
     6541 tgaatttaga aaatgacatt gtcggcaaat atatcgaaca gtttttattg aaaaaagagc
     6601 ctgaaaatcc gcaaagtaaa attactgcgg actttcttaa aaatgcaggg ttttaaccaa
     6661 ataaaggaca aataatgttt aagttttcaa cggtagaaga agcaattcaa gcaattgcac
     6721 aagggaaaat tattttagtc agcgatgatg ccgaccgtga aaatgaaggg gacttcattt
     6781 gtgcagcgga atttgcgaca ccggaaaata tcaactttat ggcgaaatat ggtaaagggt
     6841 tgatttgtac gccgatttcg acagaaattg ccaaaaaatt aaattttcac ccgatggtgc
     6901 tagagaacga agataatcat caaaccgcat ttaccgtttc tgttgaccat attcaaaccg
     6961 gcaccggtat ttccgctttt gagcgttcat taacttgcct gaaaatgctt gatgaaaatg
     7021 ctaagccgga agattttaga cgtccggggc atgttttccc attagtggca aaagacggag
     7081 gcgtattagt gcgtaacggt cataccgaag caactgtgga tttagctcgt ttggcgggct
     7141 tgaaaccggc agggctttgc tgtgaaatta tggcagaaga cggcacgatg atgcagatgc
     7201 cggaattaca ggcatttgca caagcacaca atatgccgtt tattactatt cagcagttac
     7261 aagagtatcg tagaaaacac gacagcttgg tgaatgtggt ttcggtggta aaaatgccga
     7321 ccaaatacgg tgagttcaaa gcacacagtt tcgttgaaac tatttcaggc aaagaacacg
     7381 tcgctttagt taaaggtgat attggtgatg gcgaaaatgt attatgccgt atccattccg
     7441 aatgtttaac cggcgatgcc tttggctcac aacgttgcga ttgtggtcag cagttcgcag
     7501 cggcaatgaa ccaagtggag caagagggca gaggcgtggt gctttattta cgccaagaag
     7561 gacgtggcat cggtttaatt aataaactgc gtgcttacga attgcaagat agaggaatgg
     7621 acacggttga agccaacctg gcattaggtt tcaaagatga tgagcgtgaa tattatatcg
     7681 ccgctcaaat gtttgaaaaa ttaggcgtaa aatcgattcg tttgttaacc aataatccgg
     7741 caaaaattga aggactcatt gagcaaggtt taaacgtagt ggctcgtgag ccgattatgg
     7801 ttgagccaaa cgaacacgat ttggaatacc ttaaagttaa acagcacaaa atgcgacata
     7861 tgtttaattt ctaatccaat actctctccc cttgtgggag agagacagaa aaatacttcg
     7921 ttcagaagta tttttcagag agagggtata ttttttagaa aaagtttgca atttcccctc
     7981 tccctgattt ttcttctgaa cgaagaaaaa tctatccctc tcccacaagg ggagagggtt
     8041 attgagtatc aaatttattt taaagaagga aaacaaacaa tggcaacatt tcaaggaaac
     8101 tacatcgcaa ctggtttaaa attcggtatt gtggcaacta actggcacaa gatttttatc
     8161 gatcaactgt taaacggtgc aaccgataaa ttattgcgtc acggcgtaga agaaaaaaat
     8221 atcgacaccg tatgggtgcc gggtgcatta gaaatctcaa ttgcagcgaa aaaaatggca
     8281 caaagcggtc gttatgatgc gattatctgc ttaggtgcgg tcgtgcgtgg agcaaccagc
     8341 cactatgatg tggtagtgaa cgagtcagca aaaggcatca gctcttctgc tttagaaacc
     8401 ggcgtgccga ttattaacgg tattttaacg gtggaaaatt tagagcaagc gattgagcgt
     8461 tcaggcacta aagccggtaa taaaggcgaa gagtgtgcaa tggtagcgat tgaaatggcg
     8521 aacctattaa aagccttagc gtaatcagcg ataaaatccc cttagaccca agagcctaag
     8581 gggatttttt tattttctgc gtagtaacag gctggcaatg acacaaaata ggctgaaact
     8641 gtaaattaac cagtgaccta attgtttaaa cggcgtatcg cctttggttg gcacaatggt
     8701 ggcagttagt gcatcagcag taaattgcgg tagttgttca attacttttc cgttggcatc
     8761 aacaatggcg gtaataccgg tattcgcagc tcgaagcaat ggtttgccaa gttctaacgc
     8821 tcgcattctt gccatttgga aatgttgcca aggcccgatg gaagaaccaa accacgcatc
     8881 attggtgata gttaataaat aatctgaatt ttggcttttt tggttttgtt gtaactgatc
     8941 accaaaaata atttcatagc aaatcgccat attaaatcgg ttgtttttcg ccaagaatga
     9001 tggttgaacg aaactgcctt gtgaaagatt aattggtaga ataaacactt cccgcatcca
     9061 atccagcacc gagccaaacg gcacatattc gccaaacggc acaaggtgat gtttgttgta
     9121 gcgagggttg ctgtgcaggg catattccct ctctttttcg cctaatagca cgctactgtt
     9181 aaacagttcc tcttgctcat tttgatagag tgtgccgata ataatttcac ttccgtgttg
     9241 gcgagctaag tgatcgagcg agccaagcag cgggctgatt tgattttcaa gtgccggaat
     9301 ggcggattca ggcaaaataa tcagttcact gttacctaat aggggcagaa tgagctgttc
     9361 gtaggttcgt acgctgtggt tgaaatgttg cggatcccac ttcatttttt gctcaatatt
     9421 cccttgtact aaactcacta caagcggttg tttttctgta tcaatttgca caaattggag
     9481 aaagcgagtg gcaaaactta agccaaaaat gaccgctagt accatcaatg atggcacagt
     9541 tttgttctct tgctgaataa ttttcactag atagctactt gcaactatta ccaagaaagt
     9601 caaaccttca acgcccaaaa tcggcgcaat accagcaaag gggctgtcta ttaagctata
     9661 gccgaattgt agccacggaa aaccggtaaa taccacgcct cgtaaatatt cggtaaaggt
     9721 aaaaatcgca gccaatgcaa acggattatt cagtttgagc ctgtgtgaaa ctacactaaa
     9781 cagcagatta taaatagcga gatagcacgc caacaggaat actgctatgt agcttactat
     9841 ggtaggaaca ccaccaaatt gggtcatact cacagaaacc cagtttacac cggtgcagaa
     9901 gtagccgata gaccaagcaa aggttgccca taatgcggtt tttctgtttg ccagagtggc
     9961 tgaccaaacc aagccggctg cggaagcaaa ggcaagaatc caaatatcaa aaggcgaata
    10021 ggcgaaagtg ccgattgcac ccagtatgat tgcaaggaga caagcggtta aaaaaggcgg
    10081 attttttgca aaattcataa taactcacta aataaatgag gcacgccgaa acgtgccttg
    10141 attgaaactc actatattat tcttcttcgt taggagaggt tttttccatt ttctctaact
    10201 gttcatcgga aagtgtgact cgtagctgaa tcaattggcg gctattagct gaggtgactt
    10261 taaaatcaat tccgtttaac tcaatttgct catctcgttt cgggaaataa ccgaaagcct
    10321 gcatcactaa accaccaatg gtatccacct cttcgtcatt aaagtcggta gcaaaaaatt
    10381 cattgaattt tttgatgtcg gtgagcggta ataccgcata agtatgtctt gaaagctggc
    10441 gaattggttt gacctcttct tcatcaaact catcttcaat atcacccaca atttgttcaa
    10501 gaatgtcttc aatggtgact aagcctgaaa ccgcaccaaa ttcatcaacc actactgcca
    10561 tatggaagcg ttctgaacgg aactctttta gcattcggtc taggcgtttg ctttccggta
    10621 caataaccgc agggcgtaat acctgttgca gattaaaggg ttcagagtca gaacgtaaat
    10681 atttcaataa atctttagag tgaagaatac cttcgatagt atctttctca ccccgaatca
    10741 ccggaaaacg agagtgtgcg gattcaataa ttaaatcaat acagacatct aaaggttcat
    10801 tggcatcaat aaacacaatt tgcgcacggg gaatcataat atcccgcact cgtaattccg
    10861 agatttccat tactccttcg atcatctctt tggtgtcgct gtcgatcaat tcattttcaa
    10921 cagagtctcg aatcacttcg actagatctt cacggttttt aggctcgttt tgaaaaagcc
    10981 cgctaaatag agagtgtaaa atcgattttt tttctgattt ttcggtggtg tttacacttt
    11041 gctgttcatc actcatagtt tgttccactt aaataaagta cgcttataat aagcaaattc
    11101 agtcacaaag caaataaaac attgctattt tcactcaaat tagcatagga taagcaaaaa
    11161 tctttttatt tcttcatatt ttaggagttt gaaatgaaat taaccaaaat agcattaagt
    11221 ttaacagcct tagttgtcag ttcgatgagt gtggccggga cgttaagtac atcatcaatg
    11281 gtggacattt tagcctttga cgggcaacgt gttaaatccg gcacgacttc ggttcaaatg
    11341 agtgagaata aaacgcatca agtggtagtg gaagtcggtg ggacagttga aggggatttt
    11401 ttcacctctg atcaaatcat tttgactttc caaggcagtg cggaaaatgc aaaattagaa
    11461 acgccaagat taaattcaaa gttagatctt cgtaaattcc aagagaatca gactgtggca
    11521 attaaaaccg attcaggtaa ggttatttca cataaacaag atttcttaaa aggggaaggt
    11581 ttccttgcga aaacaagagt tgaagataat ttaacgaaat ataacttagc gaaaaaaaca
    11641 gcttcagttg aacagtttgc gacggcacct cttgaagcaa aaggacaaat tgtggtggat
    11701 acaaataaag tttcacaaga agaattacag cttttattta gaaaggcaga taaagacacc
    11761 caaaaacgtt tcttagaatg ggcgaagaaa aatataaaat aaacctatat caattaataa
    11821 gcggtcattt ctcgccaatt ttttgcaagg tgttaaaaaa atgaccgctc atacaatgag
    11881 atttaccagc aataatagca gtaacgccag cctctataca tatagtctct atcgtaattc
    11941 caacgaattt cttcaagttc tacttgtgcc ttgtaatttt gccaagcaag cttataacag
    12001 gcttggaaca ttggggcatt gactttttcg gcatagcgtt tcttaaatgc tttctcttct
    12061 tgttcggttt ggctaagcgg cggattaaat tgttggtaca ttaactccgc cgtttctaca
    12121 tcacattgtt tggcgagctg aacttgcaaa tcttgttctg cccgaatttg cttcgcttta
    12181 cgttcagctt gttgctctgg agttaaagta cagctactta acataagtgc gagtgaaaaa
    12241 atgcagccta atttgagtgt tttcattgct gaatcctcta gttgaaattg gtgatttatt
    12301 ctaccgcttc taactcactt tcttcaagtt catcagccat agcactaagg gcatcacggc
    12361 taagttgtgc gagggcttta taaaaaccat tgtcgaaagc ttctattttt cctaagaatt
    12421 tgcctgcaca aggtagtaaa aattgttcga ataatatttt ttgagctaag gcagaatcga
    12481 gattatcttc gatccaagat gccgtgagaa atagtaaccc aatgtgatcg gcattttcta
    12541 attcaggcat tgcccgctct tgtctaaaag ctacaaaatt attaactgat aatccatagg
    12601 ctgaaattgt tggagaaata gccccatttt cagcaaaaag cgcttgatag cttgctgcta
    12661 attgcgtagg gttagcattc ttttgtaaga gagtcagcgc attttcactt tggctatccg
    12721 tatttaatgc ccagacttgg cgtaatcctc cttgggcaag ccagttaaat gtgccagcta
    12781 aaatagggtc ggtagggtga cgataaaata agttgccaaa caaacggcaa agcagggaaa
    12841 aatcgttaat ggtagtttcg ttcataattt gaaatagata gaaaaaagaa ttgttagtat
    12901 agcagaacac aagagaggac tgaatgaaat atataggcgc acacgttagt gcttcaggtg
    12961 gggtggaaaa tgcagttttg cgttccgtag aaattggtgc taatgcattt gcgttattta
    13021 ctaagaatca acgtcaatgg caggcaccgg cattaaaagc cgaaactatc gaaaaattta
    13081 agcgtttttg taaagtgcat catttttcag cagagcaaat cttaccacac gatagctatt
    13141 tgattaattt aggaaatccg gaagcggagg cattggagaa atctcgtgta gcatttattg
    13201 atgagatgtc tcgagcaaat caattaggtt taaaattgtt gaattttcac ccgggggcgc
    13261 atttaaagca aatttccgag caagattgtt tggctagaat tgcagaatcg atcaatattg
    13321 cagttgaaaa agtaccgaat gttattgccg tgattgaaaa tacggcagga cagggttcta
    13381 atttaggttg gcatttcgat catttggcaa ctattattga gcaagttgag gataaaagcc
    13441 gagtgggggt gtgtttagat acttgtcact tattttctgc cggctatgat attagttctt
    13501 atgaaaagag cgaggcagta tttaatgatt ttaaccaaac ggttggtttt cattatttaa
    13561 ggggaatgca cttaaatggt tcaaaaacac cattaggcag cagagtggat cgtcatcata
    13621 ccttaagaga aggcacaata ggaaccgaag tgtttgcatt tattatgcaa aatatgcatt
    13681 ttaaccatat tcctatgatt ttagagacaa tttcgcctga aatttgggca gatgaaataa
    13741 aatttctacg cacattggaa ctaaaatagg ttatactggt ctgagtagtg ggtctagcag
    13801 agtctagacc ttattttgtt atctttttta atcttttaga agatttagtt aaggaattta
    13861 ccgctgtaat tgttctggcg agacgagaca taaattatga aaaaaaatct ttttgcaaca
    13921 accttcaagc ccgtatttat tgcttctatt ttgtctgttt ttggagggta ttcttccatt
    13981 gctgtagcaa gtttaagttc taaaccggcc acaccaaatc aaatcattgc aagtaaagca
    14041 gaaagattag ctaagtcttc ttcagtattt accaatcgag caaatcttcc aacttcttcc
    14101 gctcatacag cgaattatag tggtcgtatt aatagtgttt atcataattg ggtagggaca
    14161 cgctatcgtt taggcggcac aacaaaagca ggaattgatt gttccgcctt tgtgcgtgaa
    14221 gtaatgggca aagcatttag taaagatatg cctcgttcaa ctgcagaaca acgccatgtc
    14281 gggcattcca tttcaaaatc tgatttacgc ccaggtgatt tagtcttttt ccgtaagaat
    14341 aaccatgtag gggtttatat tggtaatggt aaatttgtac acgctagttc aagtagaggg
    14401 gtaaccacca gctcattgtc agaaagctac tggtcaagaa actacacaca atctcgccgt
    14461 gtaatttaat aaattacaat tccaagatga aaagaacact cacttttgcg agtgttcttt
    14521 tttaattaaa aaagtttatg ctacgcgttg cttagcttgt gtaaatgctc cacgagtacg
    14581 catttgatga accacattag taattacacc tgctattttt acttcagccc aatcagtaat
    14641 tctaagattt gattcgctca catctaatga aaataaaatt ttttcagcaa taccttcccc
    14701 attctgaatt tcattaaaga attgatagaa tttatattcc ccatcttttt caagcaccaa
    14761 tagatcatta actaaatatt ggttggtctg ttctacaatc aataaatcgt ctagttcaat
    14821 tccccacgcg attaaatttg ggtttttcac gtggataaaa aaagtgtctc ttgggcgttt
    14881 gatacaaagt gagttaagat cgagctttac agagcggctg ctattgaaat cccgataaag
    14941 cggaatcggc tgataagctg ccgtttgatc tgaaatttgt ctaattgaag ccatagtaaa
    15001 atcctctatc tgttttttta tacagggtat tttactggct aaaataccag tgtcaaccat
    15061 tattttggaa ttttttgtaa atttaaccag tgttttgacc gcttgttatc ctttaaaaag
    15121 caataaaaaa gcacccatag ggtgcttctt atgcttaaaa gctgttatta taggctttca
    15181 gtgaatgtac gagtaataac atcgcgttgt tgctcagggg ttaatgagtt gaaacgaact
    15241 gcataacctg aaacacggat cgttaattga ggatatttct cagggttttg aatcgcatct
    15301 tctaatgttt cacggcgtaa aacgtttacg tttaagtgct gaccaccttc aactttaatc
    15361 gttggtgcaa cattaacggg tacttcacgg tatgcgatat cacctaattc gctgatagca
    15421 acaacttgat cttcttcaaa tcctgctttt gcagctaaac aacgagcttc atttttagct
    15481 tcgtctaata accagaaaga gtttaataag ttgctgtttg atgattctgt gatttgtaca
    15541 cctttaatca ttttgatatc tcctctaaga ttgaaattat aaaaaaattt taaaaattca
    15601 aatttatttg aagttattgt accccttttt agataaaaag cgataaaaat ttaaccattt
    15661 ttagacatga ttgagttggt tgggtaaaaa atatacactt ggtggtttta tgaaaacttg
    15721 gacagaagct ttaggcgaag aaaaacaaaa agcgtatttt caacaaattt tacaatatgt
    15781 acataacgaa cgattagccg gcaaagtcat ctatcctccg caaaacgagg tgtttagtgc
    15841 ttttgcctta accgaattta gtgacgtgaa agtagtaatt ttagggcaag atccttatca
    15901 cggcccaaat caagcccacg gattagcctt ttcagttaaa ccgccggttg ccccgccacc
    15961 ttctctggtc aatatgtata aagaattatc tgaagatatt gaaggatttc aaattccacc
    16021 gcatggctat ttaattgact gggcaaaaca aggggtattt atgttaaaca ccagcttaac
    16081 ggttgagcaa ggtagagccg gttcgcattc acaaattggt tgggaaattt ttaccgataa
    16141 agtgatcgca caattaaatc aacaccgtga taatttagtc tttctacttt ggggaagcca
    16201 cgctcagaga aaggggcaat ttatcgatcg taatcgccat tgtgtgttag cttctccgca
    16261 cccatcacca ctttccgcat atcgtggatt ttttggttgt aaacattttt ctaaagccaa
    16321 taactacttg atttctcaag gaatatcccc aattaattgg caattatcag tagacgttag
    16381 ttattcttca acacattagt ttaaggagca aacgatgaat tttgatttac cgattgttag
    16441 tattgcattt gttgtgcttg ttttagttgc attaagttcg actattaaaa ttgtgccaca
    16501 gggctatcat tggacagtag aacgttttgg tcgttatact aaaacgctaa gcccggggtt
    16561 gaacattgtc gtacctttta ttgatcgtat tgggcgaaaa atgaatatga tggagcaagt
    16621 tttagatatt ccatcgcaag aggttatttc acgtgataat gcaagtgttg cgattgatgc
    16681 tgtatgtttt gttcaaacag ttgatgctcg ccgagctgcc tatgaagtga atcatttaga
    16741 gcaagcaatt gttaatttaa ccatgacaaa tatgcgtacc gtgctcggct ctatggatct
    16801 agatgatatg ctctctcagc gtgatttaat taacggtaga ttactttcca ttgtcgatga
    16861 ggcgactaat atttggggag taaaagtcac ccgtattgag attcgggatg ttcgtccacc
    16921 gaaagagctg gttgcggcaa tgaacgctca aatgaaagct gagcgtaata aacgtgctga
    16981 tattcttgaa gcagaaggga ttcgacaagc tgaaattctg cgggctgaag gggaaaaaca
    17041 atctcgtatt ttaaaagcag aaggtgaaag acaagaagca ttcttacagg cagaagctcg
    17101 tgaacgagcc gcagaagcgg aagctaaagc cactcaaatg gtttctgaag caattgctaa
    17161 aggcgatact acagcgatta actattttat tgcacaaaaa tacaccgaag ctttgaaaga
    17221 aataggttct tcagataaca gtaaagttgt acttatgccg ttagaagctg gaaatttgat
    17281 tggttctgtt gctggtattg ctgaaatttt aaaaagcaat aaaacaagct catagcctaa
    17341 aaccggatta attgagtata tggagggagt atggactggt tttttaattg gattggctgg
    17401 agcatattag gctttgtact acttgcatta gaattagtat taccgggtgt ctttattatg
    17461 tggtggggat ttgcctcgct tattcttgct ttcatcgttg cactttttac attagggcaa
    17521 gcgttacaaa ttgcgttatt tgcgataatt gcgactattt ttagtcttat ttggtggaaa
    17581 tatcagcatg gaaaagacaa gaaagaggat cagagcagtt cccttaactc tcgagagcat
    17641 gctatgctag gagctcaagg ctttgtggtt gaaatcttac ctaatggcat cgctcgagga
    17701 aaatttggtg atacgacttg gcgtgttata ggtaataatt tacaagtagc agataagatt
    17761 gaagtccaga aagtggaggg aattacctta tttgtccgta agcaataagt tttttcttat
    17821 ttagtcatag aacactaaac aaaaaccttg aacagttgtt caaggttttt tctctcaagc
    17881 ggttgaaaat agagtgaaat ttgcaaattt tcaccctaaa ttaaccgctt gtgtcccacc
    17941 ataataagtt agaaactaaa gccaacattt actttccaac caaataggtt tgcagatttc
    18001 actcgactgt aatctaaatt catcgcagta aaccaatttt tataatgcag tttcaccccg
    18061 acttcgccgt ttaattggcg attacgtttc gtcgttgctt cactatgcag tgtttttcct
    18121 gcaatgttac tggtaatttt ggttattttt ccatttaatc tatgcaagta atttagcccg
    18181 atataagggg aaatagagcc ttgctcaagt tggaaagttc gctgtaattt cacgcttgga
    18241 acaatattca catcacgata acgcattgca gccgtttgag tcgcaagttc gccactttca
    18301 tttaaaccat tcattttggt ttgaatccat tgtacgccaa ggcttggttc aattatccat
    18361 tggtttggca taaattgata accgatccgc atttcaccac cgagcgttga acctctggtt
    18421 tctgcagtac ctagttgatg gctagcaaat cgacggcttt gttgatattt ctggcgagca
    18481 aattgcaatg tagcttcact aaaccaagcg ttatctaaat gataacgtaa gccaatattt
    18541 aatgctgttt gttttaaggt agcttgtgca aatggttttt ctaagcggtt tttttgctgt
    18601 gaaagtgttg cactaagtac cgttttatct gccagtttgt ggtttactcc caattgtgat
    18661 tggtggtgtt taacatcggt atgcttcgat gaggctgatt gtttttgatt caaatgttca
    18721 gcccaaacag tcgtttgcag tggtgtatgg tatgccgtaa acaaatgttg ctgtgcttgg
    18781 gttaagcctt tggcattttg gctacgtagc aattcaaggc tatctgcata aatattgtta
    18841 gatacagcat agtgcagatt atctaattta cgttgttcga gtgcagattg cagccaaccg
    18901 ttataccaac tacggcgata ggcaataggt gtagttctta acgcatttaa taatgctaaa
    18961 ccggcttgcc cgtttgtatc ggctttctca tcagttagtg gcttattcac ttttgcgata
    19021 atttcgctct ctttgaaata gagatcttgg attaaccctc gtttggctaa attctcgttt
    19081 tcgactaatt gtgtaaatcc gccttgataa gttgctttat ctttcaactg tactacggtc
    19141 gctgaaacat cacctgcttc ggttttttct gttaatttat catcaatttt taacgagccg
    19201 gctaaatgta tattggcttg agcattggca attagcccag atttgcccaa cacttctaat
    19261 tggctttgtc cagacgaaga ataattttgc tcaacttcgg ttccattgac agctaaattt
    19321 gcatggttac tgattgtttt caccttagtt ttaccgttta aggccaatct accttcttca
    19381 actgctaccg tatctaaatg attttcgccg tctaaatgca gtgatttgtc cccttttttt
    19441 gtaattttaa attggctggc gagtggattc gaccaatggt catcacgagt tacggtaatc
    19501 gtttcatcat ttaggaattg agttgggcca ttaacagctt ttttcaggtt aatgagaccc
    19561 cagccataaa cattatcgac ccctttttca cctaaatcag tggcggtagt caataaagta
    19621 tcgcgaattt gagtcgcagt cagataatca aagcgttctt ttaatactgc taatgaagca
    19681 gttatagcag gtgctgaaaa tgacgtacct tgctcttttg ttaagccata ttgtggtttt
    19741 ttatgttcat cagcaacgcc aattaaaaca tgtaaatcgc ccggtgctgc tacacaccag
    19801 tttttacttg ctccgcagtg gttagagtat gaagcaattt tttgttcttt atctaccgca
    19861 acggcagaaa ttaaattttt ctcaagctcg ggaaagtaac gtggtagaag agccattacg
    19921 cctggctgtt tcttgctctc attaccagca gcaaagacta ataaggtgtt gctatttttg
    19981 gctctcataa tagcttgaag tagcggattg tgcttatttt gcttgataga ttgataaaat
    20041 ttatctacaa cattgatgtc atcgctaata gaaaaaggat tccaactatt attgattgca
    20101 aaaacattat tatctagtaa tttattcagc ccggatgccc attcagcacg ttcaattgcc
    20161 atttcttttt ctttgcttgc taataagtca ttctttgcca tttgatccag acttggcgtt
    20221 ttttcatcct cttcgtcttt tgcattttct aattctttct tagcatcttg aataactttt
    20281 tctaaagttc gtttatatga cttcgttgca acataaagct cagctccctt tgcaatacca
    20341 ccactatagc catcaccaag agttttatct gcttgagcgg caataatacc agctacacct
    20401 gcaccgtgtg tttcaagaga aggaataaga tcaattccat cttctttttc tacggggttt
    20461 acctcgaatt gagggtaact tgcatcgtag attcttactt cgccttcggg tgttgttaat
    20521 tgatgaataa gaggatgaag atgtgaacta tggcgagggt gtttaaccat gaagccctca
    20581 tccattacgc ctagtttaac acccttgcca ctatattttg agctgaggct ctcagataat
    20641 tgaatgatag gttgtggatt ctctattgat tctgtagcgt atgcaataga taaaaaacta
    20701 gaaatgctaa ttgcaataag
//
LOCUS       DANLUQ010000025        20474 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.025, whole genome shotgun sequence.
ACCESSION   DANLUQ010000025 DANLUQ010000000
VERSION     DANLUQ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 20474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20474
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.025"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..1158
                     /locus_tag="PXB27_000766"
     CDS             <1..1158
                     /locus_tag="PXB27_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HDL5345585.1"
                     /translation="AITFIEAKVGSALQPGWYLVNQAPKSRYDSENRYVHYNKSPIKL
                     IAWGYFNGIINAATNLHTVSPNLDIEKLRQFITDLRLSFPIQAPEVTNDDMHHPNEIR
                     NLVIALNLVQDPTEHIDELPKKVYPSDLFNFGKAQKTLVGSVSIIYRNVWNEIRTQHF
                     DGEDAILKALKLVSNRIYQGNSSPQSVNVFCYSKYLRSELRDFVADLVHKCITIQTGC
                     LLQENEFSTLKVAGKTWQFLFHKKTFSIKQIDEQQAVENLNSFAKTKQNAKSENEKKK
                     EFPQQISEFASEGFLQFFFEDNVDNSFNVYVVDERNNVESYYNCFGNKEEKIRKINKL
                     QNEKQLKTETPASFGSFNHPQFYQLISPNGELCIVPFQSRQHLDYLAQKHH"
     gene            1189..3057
                     /locus_tag="PXB27_000767"
     CDS             1189..3057
                     /locus_tag="PXB27_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDL5345586.1"
                     /translation="MSGLVSPELIKTVALLATSVTIVPLFKRLGLGSVLGYLVAGCLI
                     GPSGIGLFQDPAAVVHMAELGVVMFLFIIGLEMHPELLWAMRKAIFGRGFLQVAICGT
                     LLTLAGIYLLGFSKEVAFIAGMGFTLSSTAIVMQVLEDKGISSTPKGQRIVATLLFED
                     LAIVPLLASVAFLAPNQVDAESSTNWSAIGLSLVAVLILVASGKWLMNPIFRVISKAK
                     VREMMTAAALLVVLGSALLMEISGLSMAMGAFLAGVMLSESSFRHQLEADIEPFRGLL
                     LGLFFMGVGMSLDLSLVLNNWLWLLVIVAVYVIGKGLGIFIAASITRLTLNESIVRTT
                     IMAHGGEFAFVLFSAAATAGILTPDTHATLTAAVIVSMLLSPLLILIKQGVRKKKAKN
                     ESTNMTGVELPEDLEANVLVIGFGRFNQIVCQALLAKGLSVSVIDSNTDHIRAAARFG
                     FKLYYGDGARLDVLRASGIANANCVIVGVGNPERTEKIVELIKSEYPLVPVFARTYDR
                     QSAVDLVKKHHVDYHIRETFESALALSQAAIERLGSSEEEAKQIISYVRRLDAERFNE
                     ELLHGFSAEVIRKYWVPTPWVNPQHEGSALNEETADILEELGETVEVIKVDITDKN"
     gene            complement(3217..4113)
                     /gene="malM"
                     /locus_tag="PXB27_000768"
     CDS             complement(3217..4113)
                     /gene="malM"
                     /locus_tag="PXB27_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HDL5345587.1"
                     /translation="MKKTLLSTAIFFAHLLVVSPSIANGSAGKSIHINSAELAQIQWQ
                     DVPFSQTVKTDLSEQHKQAFTTAFAGIASPVAAYRIPANQGTLEIEIESPVMDKNVFV
                     PTAVVLDSQFNVASTYPSSEFKLLEERGLKGSRLGAELNLTPAMNQDYIYLLIYTTQQ
                     DLAKTTMMPHPAKTYAKATGKQPPAINDIEVAHSLKGQIHINVTGSNGTKFIGLPTEI
                     FSSNKAATPAAKTAIPLPIKENPVAVKTAVDKDTEAYFNQAVMKAIKAKDINKAMNLV
                     NEAEKLGLTSPRQIFLKNVSSN"
     gene            complement(4194..5483)
                     /locus_tag="PXB27_000769"
     CDS             complement(4194..5483)
                     /locus_tag="PXB27_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin"
                     /protein_id="HDL5345588.1"
                     /translation="MKKTVLAVAISGAMFAASASAVDFHGYARSGIGWTSGGGEQSAF
                     TVNGGGSKYRLGNESDTYAELKLGQELFKSGEKSIYFDTNLAYGGTLHNNDWTPTSPA
                     LRELNVQFKNFADSLPGATLWAGKRFYQRHDVHMNDFYYWDISGPGAGVENIDLGFGK
                     LSLAVTRDTERGGANTFGYTTVYKLDPSTGKIKLDKQRNKADVYNDIFDVRLAGIELW
                     KDGSLELGFDYGNAHAKKGAALEKGATKNGYMVTAEYTQGNFFGGFNKFTAQYATDSM
                     TSWNNGHAQGSKADNKGNMLRLINQGVVQASDKVEVMYALIYEKTKLDNKQGKTWYSA
                     GIRPMYKWNDTMSTLLEVGYDRIKDQATGKKNDLVKYTVAQQWQAGNSIWARPAIRVF
                     GTYARWNDKFNTKARTDAGYKAKDGEFITGVQFEAWW"
     gene            complement(5581..6699)
                     /gene="malK"
                     /locus_tag="PXB27_000770"
     CDS             complement(5581..6699)
                     /gene="malK"
                     /locus_tag="PXB27_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005552148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HDL5345589.1"
                     /translation="MANVSLRNVGKSYGDVHISKDINLEINEGEFVVFVGPSGCGKST
                     LLRMIAGLEDITTGELYIGGKLMNEVEPSKRGIGMVFQSYALYPHLDVAENMSFGLKL
                     AGVSKAERDQRVNQVAEILQLAHLLDRKPKALSGGQRQRVAIGRTLVSQPEVFLLDEP
                     LSNLDAALRVQMRVEISKLHKKLNRTMIYVTHDQVEAMTLADKIVVLNAGGIAQVGKP
                     LELYHYPANRFVAGFIGSPKMNFLPVKVIAVEPERVKIELPDANHHNFWIPVSGEGVN
                     VGDNLSLGIRPEHLVPSDQTQVTLHGIVQVVELLGNETQIHLEIPEIKQPTLIYRQND
                     VVLVNEGDDLNIGILPERCHLFKEDGTACKRLFVEKGV"
     gene            7069..8259
                     /gene="malE"
                     /locus_tag="PXB27_000771"
     CDS             7069..8259
                     /gene="malE"
                     /locus_tag="PXB27_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005537801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HDL5345590.1"
                     /translation="MKNKCVKFTLATLATLVLSGTVMAKMTEGKLVIWINGDKGYNGL
                     AEVGKKFEKDTGVQVLVEHPDRLEEKFAQVASTGDGPDIMFWAHDRFGGYAQSGLLAE
                     VNVSKEFKDKFVDFAWDAETYNGKIIGYPVAIEAISLIYNKDLVKEAPKSWEEIIELD
                     KKLKKEGKNAIMWNLSEPYFTWPIAASNGAYAFKFANGKYDPKDIGVNNEGAVKALQF
                     VVDMVKNKHISADMDYAVAEASFNKGQSALTINGPWSWGNIDKSGIKYGVTLLPTLNG
                     QASKPFVGVLSAGVNSSSPNKDLAKEFLENYLLTDDGLDAVNKDKPLGAVALKSYQEK
                     LAADPRIAATMANAKNGEIMPNIPQMSSFWYAEKSAINNAVTGRQSVKEALDDAHSRI
                     QKQQ"
     gene            8378..9916
                     /gene="malF"
                     /locus_tag="PXB27_000772"
     CDS             8378..9916
                     /gene="malF"
                     /locus_tag="PXB27_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HDL5345591.1"
                     /translation="MLTQTQSKSTHWLKYLLAGLVLLLDFYLVVLMYSQGEYLFAILT
                     LIILTSGVYIFTNKNTYAWRYVYPGITGMAIFILFPLVATIAIAFTNYSGSNQLSFER
                     AVSVLTEQRYFAGDKYQFTLYPQADNKYQIALTNPTTEQTFVSEPISLATGTNVVVTE
                     QNRPTGEVAPLKVITQNRAALQSMKVVLPNNNELTMSSLRQFSEQKPRYQFDEENKIL
                     LNNETGKRYQANDETGFFQSIDENGHWQSETLEPGYTVTSGFNNFIKIFTDEGIQKPF
                     VQIFIWTVIFSLLTVVFTVILGMVLACLVQWEALKGKAVYRVLLILPYAVPSFISILI
                     FKGLFNQSFGEINMILNQLFGISPEWFNDPLLAKVMILIVNTWLGYPYMMILCMGLLK
                     AIPADLYEASAMDGASTWQNFSKITFPLLLKPLTPLMIASFAFNFNNFVLIQLLTNGR
                     PDMIGTTTPAGYTDLLVSYTYRIAFEGSGTQDFGLAAAIATIIFLLVGGLALLNIKAT
                     KMEL"
     gene            9969..10859
                     /gene="malG"
                     /locus_tag="PXB27_000773"
     CDS             9969..10859
                     /gene="malG"
                     /locus_tag="PXB27_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HDL5345592.1"
                     /translation="MAIVQSKSVRYRVWATHFILICFLALIIFPLLMVIGISLRPGNL
                     AIGDIIPSQISWEHWQAALGFDVTHADGTVTPPPFPVLRWLWNSIKVATITSIGIVTL
                     STTCAYAFARMKFKGKKTILQGMLIFQMFPAVLSLVALYALFDRLGQYVPFLGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDGSLEEAASLDGATPWQAFRLILLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLYPQNYLWGDFAAAAVLSAIPITL
                     VFLLAQRWLIGGLTAGGVKG"
     gene            10934..12991
                     /locus_tag="PXB27_000774"
     CDS             10934..12991
                     /locus_tag="PXB27_000774"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HDL5345593.1"
                     /translation="MKNALPYLCLFYTSSLFAQSWQHSHFSDFNDDLEKHFFQSQVQL
                     EKGSYPLRFKFGEQCYQPQQAIKLNQSVALVPCINEAPQLRLFRQGNYLAQIDLRSGT
                     PTPTISVEQQLQHNEAMFQSCPNWDKKPIEIDVSSTFAEGESVSDFYSGNTAIVKNGK
                     VTLMPNENASGLILLEKSNTENTATFDWKNATVYFVLTDRFYNGDPTNDHSYHRQKDG
                     MQEIGTFHGGDLKGLTEKLDYLQQLGVNVLWISSPLEQMHGWVGGGDKGDFPHYGYHG
                     YYHLDWTKLDANMGTEDDLSRFVQQAHQRGIRVLFDVVMNHTGYATLADMQEFNFGGF
                     YLTPDEISRTLGEKWTHWKPKSGQNWHSFNDFIRFNDNQAWQNWWGKEWVRADIADYD
                     SPKFDDLKMSLAALPDLKTESEQAVKLPEFFANKNTNAKTLPNAKVRDYLIGWLSDWV
                     RKYGIDGFRVDTAKHVEKSTWLVLKQSAQQALNEWQKANPNAGFNDRFWMTGEAWGHG
                     VFKSDYYQNGFDAMINFDFQDQAKQGLNCFAHIEPIYAEMNRKLSDFNVLSYLSSHDT
                     RLFFHSDSEQNIEKHKTAANLLLLSPGAVQIYYGDESGRTFGATGSDLIQGTRSDMNW
                     QELQQDPQKQALHQHWQKLTQFRQRHPAIGAGVHQLIKNDSYFAFKRVLNEDKVMVVW
                     AGN"
     gene            complement(13105..15831)
                     /gene="malT"
                     /locus_tag="PXB27_000775"
     CDS             complement(13105..15831)
                     /gene="malT"
                     /locus_tag="PXB27_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HDL5345594.1"
                     /translation="MLIPSKLVCSFRLQNSIPRKRLIQELDKSCSYPVVLINAPAGYG
                     KTTLVSQWIENKKNIGWYALDEGDNNSDRFATYFSAALHSAINEEIDVLLEENRKANL
                     LALFNQLLIKAAAFSEHFYLAIDDYHIIENDEIHEALKYWIKHQPSNMTLILISRSIP
                     PLSIASLRVQEQLLEIDINQLMFDHQESTAFFQARLGSELNQQDVIALCNEVEGWPTA
                     LQLISLFAKNKSHTLQVPLQDIAKRLAKSNHFHINEYLADEVLNKVDEETRLFILRCS
                     VLHSMNESLVQAVTGEQNSRTKLESLEKQGLFLQQMANSKWQSSDDSWWKFHPLFASF
                     LNLCCQNELYAELPQLHQKAANAWLQLGYVTEALHHAMQLSDTGLLLNILSKHAWTVF
                     HQGELKLLEESLNCLDYAHLTEHTNLVLLKAWLVQSQHRHIEVGGILADFSRALTENK
                     IELSKTANAEFNVLRAQVAINSGDENTALQLASEALNDLSENAYYAHIVATSIIGEAH
                     HCHGNLAEALAMLQKAERMARQHHTYHNILWSQLQQSEILLAQGFSQAAYEMLDKASE
                     FVKENHLQKVPMYEFLLRSKGKILWEWYNLDKAESMAVAGMNALSKFEDKLQCLTLLT
                     KISLARGNLDNTARLLGEVEQLEHSHSYHHDWIANADQVRIFYWQMTNDVSAVRNWLI
                     QNPSPVSDKNHFTQVQWRNIARSRILLEEYHEAKAILDNLIETAEKFSLISDLNRALI
                     VRNRLYFLQGEKELAQQDLIQALKLTRQTNFISAFVIEGDVMAQQIRHLLQLNVLDEL
                     VLHKAQFILRNINQFYRQKFAHFDENFVNQLLKNPKVPELLKISPLTQREWQVLGLIY
                     SGYSNEQISDELQVAATTIKTHIRNLYQKIGVTNRNEAISYTKELLMLMGYH"
     gene            15995..18364
                     /gene="malP"
                     /locus_tag="PXB27_000776"
     CDS             15995..18364
                     /gene="malP"
                     /locus_tag="PXB27_000776"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HDL5345595.1"
                     /translation="MTFNSIVEKYCRYFDVTDPKHFTLQQWYQVVAEGSLELIYSQPA
                     TKPTESRHVNYLSMEFLIGRLTGNNLMNLGYYEQIRDYLKQYQVELVDVLEQERDPAL
                     GNGGLGRLAACFLDSMATVGQNATGYGLHYQYGLFKQSFKEGMQKEAADTWARDSYPW
                     HRFNPSKTRHIGFGGKIKHIQADQYEWQPKLTIQGKAFDLPIVGYKNNIIQPLRLWQA
                     DSDQSFDFDAFNEGKFLSADKTIVNAAALTQVLYPNDNHQAGQKLRLMQQYFHCACSV
                     ADILDRHISEGYSLEDLAKRQVIQLNDTHPTLAIPELMRVLLDEHQFTWEQAWDICSN
                     TFAYTNHTLLPEALEQWDQRLFKQLLPRHYQIVEKINDIFHDKVQAEFGDDAKIWEKL
                     AVLFDYRVRMANLCVVTCFRVNGVAEIHSDLLVTDLFPEYHKLFPTKFCNVTNGITPR
                     RWIRQANPQLSDLLDRTLKQDWTHNLELLKGVEQYADDTGFREEYQRIKRNNKVVLAN
                     EIHKTLGLNVNPNAIFDVQIKRFHEYKRQHLNLLNIIATYQSLKANPNQDYTPRLFLF
                     SGKAAPGYYLAKNIIHAINNVADIINNDKQVNDRLQVAFLPDYRVSLAEKIIPAADVS
                     EQISMAGKEASGTGNMKLALNGALTLGTLDGANVEIADMVGEENVFIFGHTVESVREL
                     LAKGYQPKAYYNQDPILKNAVDFLATGKASNGDKEAFKLMLDSLLERDPFLVFADFDS
                     YRQAQEKIGVAYLDQETWLRSAILNTARLGAFSSDRSIKDYQQRIWLNR"
     gene            18446..>20474
                     /gene="malQ"
                     /locus_tag="PXB27_000777"
     CDS             18446..>20474
                     /gene="malQ"
                     /locus_tag="PXB27_000777"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005565649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HDL5345596.1"
                     /translation="MKITAKQYYRAGIMPYFFDERGVKKTASEKIKHSLFKAFNGNTQ
                     ARETPIPTVNIFYQHQPCFLPFHLADKKHPLTGTWQLQLENSNQIISGKIKARGITLP
                     QDLPLGYHQLQLKTVDKTFNCTIIIAPERCYQPQAVIEQKRLWGTFLQLYTLKSAHNW
                     GIGDFGDLKDFIQQIAPYQADFIGLNPIHSLFPANPNSASPYSPSSRRWLNIAYIDIN
                     QLAEFQQSDEAQTWFHSAEIQQQLSELRTQDWLNYEQIIPLKLKGLRFAFAKFQQNMT
                     ACAAPAFIEFVQKGGESLQVQATFDALHQHLSAQFADQWGWDFWEKAYQDYHSAAVQQ
                     FRETHKTDIEFYLWLQFVADQQLAECDAICRQQNMRIGMYRDLAVGVSPNGAETWNDK
                     QLYCLNASVGAPPDVLGPQGQNWGLTPMNPHVLKQQAYRPFIELVRANMSHCGALRLD
                     HIMSLLRLWWIPKGDSAVNGAYIRYPVDDLIAILALESQRHQCLIIGEDLGTVPKEIV
                     SKLKNAGILSYKIFYFEFDEQGKSRDLQHYPYQAMTTLSTHDLPTINGYWRGYDFELG
                     EKFGVYPNSNILAILKQDRVNAKAKILQRLQEHNVEIAPEISEQLNSPVNTAFIHCLQ
                     TYVANVSSGLFGFQPEDWLGMTEPVNIPGTSTQYANWRRRLSVNLEEIFTDK"
BASE COUNT         6129 a         4159 c         3997 g         6189 t
ORIGIN      
        1 gcaattacat ttattgaagc taaagttgga tctgcgttac aacccggttg gtatttagtt
       61 aatcaggcac ctaaaagtcg ttatgactct gaaaatcgtt atgtccatta taataaaagc
      121 ccgattaagc ttattgcttg ggggtatttt aacggcatta ttaacgcagc aactaattta
      181 catactgtta gccctaattt agatatcgag aaattgcgac aatttatcac cgacttgcgc
      241 ttatctttcc caattcaagc gccggaagtg accaatgatg acatgcatca ccctaatgaa
      301 attcgcaatt tggttatagc gcttaattta gtacaagatc caaccgaaca tatcgatgaa
      361 ctaccaaaaa aagtttatcc aagcgatctg tttaattttg gtaaggcaca aaaaacgtta
      421 gttggtagtg tgagcattat ttatcgaaat gtatggaatg aaattcgaac tcaacatttt
      481 gacggtgaag atgctatttt aaaagcacta aaattggtat ccaatcgaat ttatcaagga
      541 aattcttcac ctcaatcagt taatgttttt tgctatagta aatatctacg tagtgagtta
      601 cgtgattttg tggcagattt agttcataaa tgtattacaa ttcagactgg ttgtttactg
      661 caagagaatg aatttagcac tttaaaagta gcaggaaaaa cctggcagtt tctttttcat
      721 aaaaaaacat ttagcattaa acaaattgat gaacaacaag cggttgaaaa tttaaattct
      781 tttgcaaaaa ccaagcaaaa tgctaaaagc gaaaatgaaa agaaaaaaga atttccgcag
      841 caaattagtg aatttgccag tgaaggcttt ttacagtttt tctttgaaga caatgtcgat
      901 aatagtttta atgtatatgt tgtagatgaa cgtaataatg tcgaaagtta ctataactgt
      961 ttcggcaata aagaagagaa aatcaggaaa atcaataaat tacaaaatga aaaacaatta
     1021 aaaacagaaa caccagcatc atttggaagc tttaatcatc cacaatttta tcaacttatt
     1081 tcaccaaacg gtgagttatg tattgtgccg tttcaaagcc gccagcattt agattattta
     1141 gcacaaaaac atcattaatc ttatttatat tatacaggtg ggaattctat gtcaggatta
     1201 gttagtccgg aattaattaa aacagtcgca ttacttgcaa ccagcgtcac tattgtaccg
     1261 ctatttaaac gccttggttt aggaagcgta ttgggttatt tagtcgcagg atgtttaatc
     1321 gggccatcgg gcattggatt gttccaagac cctgccgctg ttgtgcatat ggcagaactt
     1381 ggcgtggtta tgtttttgtt tattattggt ttggaaatgc acccggaact gctttgggcg
     1441 atgcgtaaag ccatcttcgg gcgaggtttc ttacaagttg cgatttgcgg tactttatta
     1501 accttagccg gcatttactt attaggtttt tccaaagaag tggcatttat tgccggtatg
     1561 ggttttacgc tatcttctac tgccattgta atgcaagtgc tggaagataa agggataagt
     1621 tccacgccaa aagggcaacg tattgttgca actttactat ttgaagattt agcgattgtg
     1681 ccattattag cctcggttgc ctttcttgct cctaaccaag tagatgctga atccagtact
     1741 aattggtctg ctattggtct tagcctagtt gccgttctta ttttggttgc gagtggaaaa
     1801 tggctgatga accctatttt ccgtgttatt tctaaagcca aagtacgaga aatgatgact
     1861 gccgctgcct tacttgtggt attaggctcc gctcttttaa tggaaatcag tgggctttca
     1921 atggcaatgg gggcattcct tgccggagta atgctttcgg agtcttcttt ccgccatcaa
     1981 ctagaagctg atattgaacc cttccgtggt ttacttttag gcttattctt tatgggcgta
     2041 ggaatgtcat tagacctcag cctcgtcctc aataattggc tatggttatt ggtgattgta
     2101 gcagtttatg ttatcggtaa aggccttgga atcttcattg ctgcctctat cacccgatta
     2161 acactgaatg aatctattgt gcgaactacc attatggctc acggtggtga atttgccttt
     2221 gtgctattct cagccgccgc aaccgctggg attttaaccc cagatactca cgctacctta
     2281 accgctgcgg taattgtctc aatgttgctt tccccacttc tcattttaat caagcaagga
     2341 gtgagaaaga aaaaagcgaa aaatgaatcc actaatatga caggggttga attaccggaa
     2401 gatttagaag ccaatgtatt agtcatcggt tttggacgct ttaaccaaat agtctgccaa
     2461 gccttattag ccaaaggact aagtgtatcg gttattgatt caaatactga tcatattcgt
     2521 gctgctgctc gttttggttt taaactttac tatggtgatg gagcgagact agatgtactt
     2581 agggcatctg gcatcgccaa tgcaaactgc gtaattgttg gcgtaggcaa tccggaaaga
     2641 acggagaaaa tcgtagagct gattaaatct gaatacccct tagtacctgt atttgcgaga
     2701 acctatgata gacaaagtgc ggttgattta gtcaaaaagc accacgttga ttatcacatt
     2761 cgagaaacct ttgaatcagc cctcgcatta agccaagccg ccattgaacg cttaggttct
     2821 agcgaggaag aggcaaaaca aattatcagc tatgttcgcc gattagatgc agaacgcttt
     2881 aatgaggaat tattacacgg tttctccgca gaagttatcc gcaaatattg ggtaccaaca
     2941 ccttgggtaa atccacaaca cgaaggtagt gcacttaatg aagaaacggc tgatatttta
     3001 gaagagcttg gtgagacagt agaagttata aaagtggata tcactgataa aaattaaaga
     3061 atttatactt tgaatcctta ttcaagtata agccttccta tacatatcgc ataatcactc
     3121 gtaaaaaagc accctatcgg cataatctga tagggtgtta atggtaataa accatcgcac
     3181 gatgacaagg agaatatcta tgaaaaaaac aataaatcaa tttgaactta cattttttaa
     3241 gaaaatttgg cgtggagaag tcagccctaa tttttctgcc tcgttaacca aattcatcgc
     3301 tttattaata tcttttgctt taatcgcttt cattaccgcc tgattaaaat aggcttccgt
     3361 atctttatct actgctgttt ttactgcaac tggattttct tttatcggca acggtatagc
     3421 cgttttagct gccggcgttg ctgctttgtt tgagctgaaa atttcagtcg gtaatccaat
     3481 aaatttagtg ccgttcgagc cggtaacgtt gatgtgaatt tgccctttta aactgtgtgc
     3541 tacttcaata tcgttaattg ctggtggttg tttaccggtt gcttttgcat aggtttttgc
     3601 cggatgcggc atcatcgtgg ttttggctaa atcttgctgc gtggtataaa tgagcaagta
     3661 aatataatct tggttcattg ccggcgttaa attaagttct gcccctaaac ggctaccttt
     3721 gagtcctcgc tcttccaata atttaaactc agaagagggg taagttgatg ccacattaaa
     3781 ttgactatcc aacacgactg ccgtgggtac aaaaacattt ttgtccatca ccgggctttc
     3841 gatttcaatt tccaatgtgc cttgatttgc cggaatacga taagctgcaa ccgggctggc
     3901 aataccggca aacgctgtgg taaatgcttg tttgtgttgc tcgcttagat ctgtttttac
     3961 ggtttgtgaa aatggcacat cttgccattg aatttgagct aattcagccg agttaatatg
     4021 aatagactta cctgccgagc catttgctat actcggagac accaccaaaa gatgggcgaa
     4081 gaatattgct gtcgaaagaa gcgttttttt catattaacc cctaaaaatc gtgaaatagg
     4141 ataaagtgct gaaatagtgg gtaggtaatc ctacccacac aaaggtttga tgattaccac
     4201 caagcttcaa attgaacacc ggtgataaac tcgccgtctt tggctttata gccggcatcg
     4261 gtacgagctt tcgtgttgaa tttatcattc caacgtgcat aagtaccaaa cacacggatt
     4321 gcagggcgag cccaaatgct attaccagcc tgccattgtt gtgcaacggt gtattttacc
     4381 aagtcatttt tcttgcctgt tgcttgatct ttaatgcgat cataaccaac ttctaataat
     4441 gtactcattg tatcattcca tttgtacatt gggcgaatac ctgctgagta ccaagtttta
     4501 ccttgtttat tatctagttt tgttttttcg tagattaagg catacattac ttcaacttta
     4561 tcactcgctt gcactacccc ttggttgatt aaacgaagca tattaccttt attatctgct
     4621 ttcgaaccct gtgcgtgccc attattccaa gaagtcatag agtcggttgc atattgtgca
     4681 gtaaatttat tgaaaccacc aaagaagtta ccttgagtgt attccgctgt taccatataa
     4741 ccgtttttgg ttgctccttt ttcaagggct gcacctttct tagcatgtgc attaccgtaa
     4801 tcaaagccta actctaacga accatctttc caaagctcaa taccggctaa ccgaacatca
     4861 aaaatatcgt tgtaaacatc ggctttatta cgctgcttat ctaatttaat tttacctgtg
     4921 ctaggatcta atttataaac tgtagtatat ccaaaagtat tcgcccctcc acgctctgta
     4981 tcacgagtaa ccgctaaaga gagttttccg aagcctaaat caatattttc aacccctgca
     5041 cccggacctg aaatatccca gtaatagaag tcattcatat gtacatcatg gcgttggtaa
     5101 aaacgtttac cagcccataa ggtggcacct ggtaaactat ctgcaaagtt tttgaactgt
     5161 acatttaatt cacgtagtgc tggacttgtt ggtgtccaat cattattgtg aagcgtaccg
     5221 ccgtaagcaa ggtttgtatc aaagtagata gatttctcac cacttttgaa taattcctga
     5281 cctaatttca attctgcata ggtatcagat tcgttaccta aacggtattt tgaaccacca
     5341 ccatttaccg tgaatgcgga ttgctccccc ccccccgatg tccaaccgat acctgaacgt
     5401 gcatatccgt ggaaatcaac cgctgatgca ctggctgcga acattgcacc actgattgca
     5461 actgctagga ctgttttttt cataatagaa cctctcttaa gatttgattt tacttaggtt
     5521 aattattaat tgattagaag attgaaaata ccctctaaaa tcagtaataa actgaaattg
     5581 ttatacccct ttttcaacaa acaaacgttt gcaagctgta ccgtcttcct taaataaatg
     5641 gcagcgttcc ggcaatatac cgatattcag atcgtcgcct tcattaacta ataccacatc
     5701 gttttgacga taaattaagg ttggttgttt gatttcaggg atttcaaggt ggatttgtgt
     5761 ttcatttccg agcaattcca caacttgcac aataccgtgt aaggtcactt gggtttgatc
     5821 cgaaggaact aaatgttccg ggcggatacc taaagataag ttatctccca cattcacccc
     5881 ttcgccggaa accggaatcc aaaaattatg atgattcgca tcaggtaact caatttttac
     5941 ccgttccggc tctaccgcaa tcacttttac cggtaaaaag ttcattttag gcgagccgat
     6001 aaaccctgct acaaagcgat ttgccgggta atgataaagt tctaacggtt tacctacttg
     6061 agcaataccg ccggcattta ataccacgat tttgtccgct aatgtcattg cttcaacttg
     6121 atcgtgcgta acataaatca ttgtgcggtt tagtttttta tgtaatttgg aaatttctac
     6181 ccgcatttgt acacgcaatg cggcatcaag gttggaaagt ggttcatcga gtaaaaatac
     6241 ttccggttgg gaaaccagtg ttctaccaat agccacccgt tggcgttgtc cgccggaaag
     6301 tgctttaggt tttcgatcta ataaatgggc aagctgtaag atttcggcaa cttggttaac
     6361 tcgttgatcc cgctctgctt tacttacacc tgccagcttt aagccaaaag acatattttc
     6421 ggcaacgtct aaatgtggat agagagcata ggattggaac accataccga taccacgttt
     6481 tgaaggctct acttcattca tcagttttcc accgatatag agttctcctg tcgtaatatc
     6541 ttccaaacct gctatcattc gtaatagcgt agatttgcca caacctgacg gtccaacgaa
     6601 aacaacgaat tctccttcgt taatttctaa attaatatcc ttagagatat gaacatcacc
     6661 atacgatttt cccacgttac gtaatgatac attcgccata actaaacctc ttttctgctt
     6721 ttgcttgttg gttttattta ggctgaatag tcgccttttt atctaaagaa gaaatcatcc
     6781 taaaatactt ttttttaggg ttagagccat agccatagcc ataattattt aaaaaacgcc
     6841 aattcagaaa aattaaataa attttgtgat caacctctca tttttaatgc ctatttttgt
     6901 gaagcagatc acaaaatgat aaaaaatgtt acttagatca tacttttagc ttggggggcg
     6961 tagagcaaag ggtgatctca aatagattgt atagcccata atttgccata atttaataaa
     7021 tgggttgttt catatccgtt tcttttattt aacttcaagg agttttttat gaaaaacaaa
     7081 tgtgtcaaat tcaccttagc tactcttgct accttagttc tttcaggaac agtaatggca
     7141 aaaatgacgg aaggtaaact cgttatctgg attaacggtg ataaagggta caatgggctt
     7201 gccgaagtcg gtaagaaatt tgaaaaagat accggtgttc aagttttagt tgaacacccg
     7261 gatagacttg aagaaaaatt tgcacaggtt gcctcaactg gtgacggtcc ggatattatg
     7321 ttctgggctc acgaccgctt tggtggttat gcacaatccg gtttattggc tgaagtgaat
     7381 gtaagcaaag aatttaaaga taaatttgtc gattttgctt gggatgctga aacttacaac
     7441 ggcaaaatta tcggttatcc ggttgcgatt gaagcgattt cattaattta taataaagat
     7501 ttagtgaaag aagcaccaaa atcttgggaa gaaattattg agttagataa aaaactcaaa
     7561 aaagagggca aaaatgccat tatgtggaac ttatccgagc cctatttcac gtggccgatt
     7621 gcggcttcaa acggagctta tgcattcaaa tttgctaacg gcaaatacga tccaaaagat
     7681 attggtgtga acaatgaagg ggcagtaaaa gcactacaat ttgtagtcga tatggtgaaa
     7741 aataaacata ttagtgcgga tatggactat gccgttgccg aagcctcctt taataaagga
     7801 caaagtgcct taaccatcaa tggtccttgg tcttggggca atattgataa aagcggtatt
     7861 aaatatggtg taactttact cccaacatta aacggtcaag cttctaaacc attcgtaggg
     7921 gtgttaagtg ccggtgtaaa cagctcaagc ccaaataaag atttagccaa agaattctta
     7981 gagaattatt tactcaccga cgacggttta gatgcagtca ataaagataa acctttaggt
     8041 gcggtggcgt taaaatctta ccaagagaaa ttagcggctg atcctcgtat tgctgcaacg
     8101 atggcaaatg cgaaaaacgg tgaaattatg ccaaatattc cgcaaatgtc ttctttctgg
     8161 tatgccgaaa aatctgctat caataatgca gtgaccggtc gtcaatcggt gaaagaagca
     8221 ttagatgatg cacattcacg cattcaaaaa caacaataat tcttcatcat tctgagtggg
     8281 ttattatgac ccactctatt tttagattca ccctaaatac aataaaagta cagtaaaagt
     8341 accgtaacaa aatgcagtaa aaattcgagg tgctgttatg ctaactcaaa ctcaatccaa
     8401 atccactcat tggctcaaat atctcttagc cggattagtc ttgctccttg acttctatct
     8461 tgttgtctta atgtactcac aaggtgaata tctttttgct attttaacgc tgattattct
     8521 aacttccggt gtctatattt ttaccaataa aaatacctat gcttggcggt atgtttaccc
     8581 tggcattacc ggaatggcaa tttttatttt gtttccgctg gttgcgacta ttgcaattgc
     8641 gtttaccaac tatagtggtt ctaatcaatt atcctttgaa cgagcggtat ccgtcttaac
     8701 tgaacaacgc tattttgccg gagataaata tcaattcacc ctttatccgc aagcagataa
     8761 taaatatcaa attgcattaa ccaatccgac gacagagcaa acttttgtgt cagagccgat
     8821 ttcattggct accggaacga atgtggttgt aaccgagcaa aacagaccaa ctggcgaagt
     8881 cgctcccctt aaagtgatta cgcaaaatcg ggccgcactt caatcaatga aagtggtttt
     8941 accgaataat aatgaactga caatgagctc attacgtcaa ttctccgagc aaaaaccacg
     9001 ctatcaattt gatgaagaaa ataaaatatt actcaataat gagactggca aacgttatca
     9061 agcaaatgac gaaaccggtt tcttccaatc gattgatgaa aacggtcatt ggcaaagcga
     9121 aacacttgaa ccaggttata ctgtcacatc aggctttaat aacttcatta aaatttttac
     9181 cgatgaaggt attcaaaaac cttttgtcca aatatttatt tggacggtga tcttctcttt
     9241 attaaccgtt gtctttaccg ttattctcgg tatggttctt gcctgtttgg tacaatggga
     9301 ggcattaaaa ggtaaagcag tctatcgtgt cttattgatt ttaccttatg ccgtaccatc
     9361 atttatttcg attctgattt ttaaaggact atttaaccaa agttttggtg agatcaatat
     9421 gattttaaac caactattcg gcatcagccc tgaatggttt aacgatccgc tccttgccaa
     9481 agtgatgatc ttaattgtca atacgtggtt aggttatcct tatatgatga ttttatgtat
     9541 ggggttactc aaagctattc cggctgattt atatgaagcc tccgcaatgg acggtgcttc
     9601 cacttggcaa aacttcagta aaatcacttt cccattactg ttaaaaccac ttactccgtt
     9661 aatgattgct tcgtttgcat ttaactttaa taactttgta ttaattcaac tattaacaaa
     9721 cggtcgcccg gatatgattg gaacaaccac tccagccggt tataccgatt tattagtcag
     9781 ctacacctat cgtattgcct ttgaaggaag tggtacgcaa gatttcggct tagcagccgc
     9841 catcgcaacc attattttct tattagtcgg tggcttggca ctcttaaata tcaaagcaac
     9901 caaaatggaa ttataaaagc gggagggatt taacaatccc gaccccaaac taactttagg
     9961 agttatatat ggcaattgtt caatctaaat ctgtgcgtta tcgggtatgg gcaacccatt
    10021 tcatcttaat ctgtttcctt gccttaatta ttttcccttt attaatggtg attggcattt
    10081 cgttacgacc gggaaatctg gctatcggtg atattattcc aagccaaatt tcgtgggaac
    10141 attggcaagc cgctcttggt tttgatgtta cacacgccga tggtacagtc acaccgccgc
    10201 cattcccggt gctacgctgg ttatggaact ccatcaaagt ggcaacaatt acctctatcg
    10261 gcattgtaac cttatctacg acctgtgctt atgcatttgc ccgaatgaaa tttaaaggca
    10321 aaaaaaccat tctacaaggt atgctgattt tccaaatgtt cccggcagtg ctttccttag
    10381 ttgccttata tgcgttgttt gatcgtcttg ggcagtatgt accgtttctt ggtttaaaca
    10441 cacacggtgg tgttattttt gcctacttag gcggaattgc cttacacgtt tggacaatca
    10501 aaggctactt tgaaacgatt gatggttcat tggaagaagc cgcttccctt gatggtgcaa
    10561 ccccgtggca agcgttccga ttaatcttat taccgctttc agtgccgatt ttggcggtag
    10621 ttttcattct ttcatttatt gccgccatta ccgaagtacc ggttgcctca ctcctattgc
    10681 gtgatgtaaa cagctacacc ctagccgttg gtatgcaaca atacctctat ccgcaaaact
    10741 atttatgggg tgacttcgca gcagcggcgg tcttatccgc aattccgatt acgctggtct
    10801 tcttattggc acaacgctgg ttaatcggtg gtttaactgc cggcggcgtg aaaggctagt
    10861 ttgcaaaaaa ttctctaaaa acgaccgctt gtcgagctga tactgcggtc gtcaatcttt
    10921 attagggctg tctatgaaaa atgcgttacc ttatctctgc ttattctata cctcaagcct
    10981 ttttgcccaa tcttggcaac attcccattt ttctgatttt aacgatgatc tcgaaaaaca
    11041 tttttttcaa tcccaagttc aattggaaaa agggagctat ccgcttaggt tcaaatttgg
    11101 cgaacaatgt tatcaacccc aacaagcgat aaaattaaat caatcggttg cgttagtccc
    11161 ttgtataaat gaagctccac aactgcggtt gtttcgtcaa ggcaactatc tggcacaaat
    11221 tgatctgcga agcggcactc cgacacccac aatcagcgta gaacagcagc tacagcacaa
    11281 tgaagcgatg tttcaaagtt gcccgaattg ggataaaaaa ccgattgaaa ttgatgtttc
    11341 ttccactttt gccgaaggcg aatcggttag cgatttttat tcaggcaaca ccgctatcgt
    11401 taaaaacggc aaagtcacct taatgcccaa tgaaaacgca agcggtctga ttttgctgga
    11461 aaaaagcaat accgaaaata cggcaacgtt tgattggaaa aatgccaccg tctattttgt
    11521 gctgaccgac cgcttttata acggcgatcc gaccaatgac cacagctacc accgccaaaa
    11581 agacggaatg caagaaatcg gcacctttca cggtggtgat ttaaaaggat taaccgaaaa
    11641 gctcgattac ctacaacaac ttggggtaaa cgtattgtgg attagctctc cgttagaaca
    11701 aatgcacggc tgggttggcg gcggtgataa gggggatttt ccgcattatg gctatcacgg
    11761 ttactatcat ttagattgga cgaaattaga tgccaatatg ggtactgaag acgatttaag
    11821 tcgctttgtt caacaagccc accagcgtgg tattcgggtg ctatttgatg tggtgatgaa
    11881 ccataccggc tacgcgacgc ttgccgatat gcaggagttt aactttggcg gattttactt
    11941 aacaccggac gaaattagcc gcacattagg cgaaaaatgg acacactgga agccgaaatc
    12001 aggacaaaat tggcacagtt tcaatgattt cattcgcttt aacgataatc aggcttggca
    12061 aaattggtgg ggaaaagaat gggttagagc tgatattgcc gattacgaca gccctaaatt
    12121 tgatgattta aaaatgtcgc tcgctgccct tcctgatcta aaaaccgaga gcgaacaagc
    12181 ggtcaaatta cctgaatttt ttgcaaataa aaataccaat gccaaaacat taccaaacgc
    12241 caaagtgcgt gattatttaa ttggttggtt atccgattgg gtcagaaaat atggcattga
    12301 cggttttcgg gttgataccg ccaaacacgt tgaaaaatcc acgtggctgg tattaaaaca
    12361 atcggcacaa caagccttaa acgaatggca aaaagccaat ccgaacgccg gttttaacga
    12421 tcgcttttgg atgacaggag aagcgtgggg acacggcgtg ttcaaaagtg actattatca
    12481 aaacggtttt gatgcaatga tcaatttcga ttttcaagat caggctaaac aagggttaaa
    12541 ttgctttgcc catattgagc cgatttatgc cgaaatgaat cgcaaattaa gcgacttcaa
    12601 tgtgttgagc tatttatctt cacacgatac tcgattattt ttccattcgg atagtgaaca
    12661 aaatatcgaa aaacacaaaa ccgccgcaaa tttgctgttg ctttcacccg gtgcagtgca
    12721 aatctattat ggtgatgaaa gtgggcggac atttggtgcg accggttcag acctgataca
    12781 aggcacccgg tcggatatga attggcaaga gttgcagcaa gatcctcaga aacaggcatt
    12841 gcatcaacat tggcaaaaat tgacacaatt ccgccaacgc catccggcta ttggcgcagg
    12901 tgtccatcaa ctcatcaaaa atgatagcta ctttgcattt aagcgtgtgt tgaatgaaga
    12961 taaagtgatg gtggtttggg ccggaaatta aatctacttt agcttcaagt tttctctctt
    13021 ctggggaaaa cttgaagccg gttcggggaa acaagttgca aaaaattact aaaaatagac
    13081 cgcttgttcc ccatgagctt gtgatcaatg ataccccatc aacatcagca gttctttcgt
    13141 atagctgatt gcttcgttac gattcgtcac accgattttc tgataaagat ttcggatatg
    13201 ggttttaatc gtggttgcag ccacttgcaa ttcatctgaa atttgttcat tgctatatcc
    13261 ggagtaaatc aaacccaata cttgccattc ccgctgagtg agcggactaa tttttaagag
    13321 ttccggcact ttcggatttt tcaataattg attcacaaag ttttcatcaa aatgggcaaa
    13381 tttttggcga taaaattgat tgatgttgcg taaaataaac tgtgctttgt gcaataccag
    13441 ttcatctagc acattaagtt gcaataaatg gcggatctgc tgtgccatta catccccttc
    13501 aatcacaaac gcactgataa aattcgtttg gcgggtgagt tttagtgcct gaattaaatc
    13561 ttgctgagcc aattctttct cgccttgtaa aaaataaagc cggttacgaa caattaatgc
    13621 gcgatttaaa tcgctaatca gagaaaattt ttcagccgtt tcaattaaat tatctaaaat
    13681 ggctttcgcc tcgtgatatt cctcaagcaa gattcgagac cgtgcaatat ttcgccattg
    13741 cacttgggta aaatgatttt tatctgaaac cggtgagggg ttttgaatta accagttcct
    13801 caccgcagaa acgtcattgg tcatctgcca ataaaatata cgtacctgat cggcgttggc
    13861 aatccaatcg tggtgataag aatgagaatg ttctaattgt tcgacctcgc ctaataatcg
    13921 ggcagtgtta tccaaattgc ctcgagccaa cgagatcttc gtgagtaaag ttaagcattg
    13981 taacttatct tcaaatttag ataaggcatt cattccggca actgccattg attcagcttt
    14041 atctaagtta taccattccc ataaaatctt gcctttagaa cgtagcaaaa attcatacat
    14101 cggcactttt tgtagatggt tttctttcac aaactcgctt gctttatcta acatttcata
    14161 ggctgcttgt gaaaaccctt gggcaagcaa aatttcagat tgttgaagct gtgaccacaa
    14221 aatattgtgg taagtatgat gctgacgtgc catacgctct gctttttgca acattgctaa
    14281 tgcttctgcc agattgccgt ggcaatgatg agcttcgcca ataatagacg ttgcaacaat
    14341 atgagcataa taggcatttt ctgaaagatc gtttaacgct tcagacgcta attgaagtgc
    14401 ggtattctca tcaccggaat tgatagcaac ttgtgcccgt agcacattaa attccgcatt
    14461 ggcagttttg ctcaattcaa ttttattttc agttaatgca cgagaaaaat ccgctaaaat
    14521 accacctact tcaatatggc ggtgctggct ttgtaccaac caggctttga gtaacaccaa
    14581 attagtatgt tcggttaaat gggcgtaatc taagcaattt aagctctctt ccaataattt
    14641 gagttctcct tgatgaaaca ccgtccaagc gtgtttgctc aaaatattga gtagtaatcc
    14701 ggtatccgat aactgcattg cgtgatgtaa ggcttctgta acatagccta attgtaacca
    14761 cgcattcgcc gccttttgat gcagttgcgg taattcagca tataactcat tttggcaaca
    14821 taaatttaaa aaggaagcaa aaagcggatg gaatttccac cagctatcat cactactctg
    14881 ccacttactg tttgccattt gttgtaaaaa caaaccttgc ttttcgagag actctaattt
    14941 ggtgcgactg ttttgctccc ctgtaacagc ctgcactaaa gactcattca ttgaatgcaa
    15001 aaccgaacaa cgcaaaataa acaaacgagt ctcttcatcg actttattaa gcacttcatc
    15061 ggctaaatat tcgttaatat gaaaatggtt tgatttggct aaacgtttgg caatatcttg
    15121 cagtggtact tgtagcgtgt gtgatttatt ttttgcaaaa aggctaatta gctgaagggc
    15181 tgtgggccac ccttcaactt cgttacacag tgcgataaca tcttgctgat ttaactccga
    15241 acctaatctt gcttgaaaaa aagcggtact ttcttggtga tcaaacatta actggttaat
    15301 atcaatttcc aataattgtt cttgcactct taaactggca atacttaacg ggggaattga
    15361 tcgagaaatc aagataagcg tcatattaga cggttgatgt ttaatccaat attttaatgc
    15421 ttcgtgaatt tcatcatttt caataatatg ataatcatca atagcaagat aaaaatgctc
    15481 tgaaaatgcc gcggctttaa tcaataattg attaaataaa gctaataaat tagctttacg
    15541 attttcctct aacaatacgt ctatttcttc attgattgct gaatgcaatg ccgcactaaa
    15601 ataagtggca aaacgatctg aattattatc gccttcatct aacgcatacc agccgatatt
    15661 ctttttattt tcaatccatt gggaaaccag agtggttttg ccataacctg ccggtgcatt
    15721 tatcaatacg accggataac tgcaagattt gtctaattct tgaattaaac gttttcgagg
    15781 aatgctgttt tgtaatcgaa aagaacaaac aagttttgat ggtattaaca taataaattc
    15841 aattttctct cataacaaaa tagggatgat atatccacca tttgtgttct cactacttcc
    15901 ttataatcat accatacaag atgattattg ggatgatttg attttatttg gattttacat
    15961 aatagcgaca ttcatcttta accgggggtt tcctatgaca tttaattcta ttgtagaaaa
    16021 atactgtcgc tactttgatg tgacagatcc aaaacacttt accttacagc agtggtatca
    16081 agttgttgct gaaggttccc ttgagctaat ttatagccag cctgcaacca aaccgacaga
    16141 atcacgccac gtgaactacc tctcaatgga gttcttaatc ggtcggttaa ccggcaataa
    16201 tttaatgaat ttaggttatt acgaacaaat tcgtgactat cttaaacaat atcaagttga
    16261 attagtagat gttttagaac aagagcgtga tccggcattg ggtaatggcg gattaggtcg
    16321 tcttgctgcc tgctttttag attcgatggc aactgtcgga caaaatgcaa ccggctatgg
    16381 gcttcattat caatatggtc tgtttaaaca aagcttcaaa gagggaatgc agaaagaagc
    16441 agcggatact tgggctcgtg acagctaccc gtggcatcgt tttaatccgt ctaaaacccg
    16501 ccatatcggg tttggtggaa aaatcaaaca tatccaagcc gatcaatatg aatggcagcc
    16561 taaattgacc attcaaggca aagcctttga cttgccgatt gtaggttata aaaacaacat
    16621 cattcaacca ttacgtttat ggcaagcaga tagtgaccaa tcttttgatt ttgatgcctt
    16681 taatgagggt aaatttttaa gtgccgataa aacgattgtc aatgcagccg cactaactca
    16741 agtgctgtat ccaaacgaca accatcaagc cggacaaaaa ttacgcttaa tgcagcaata
    16801 cttccattgt gcctgctcgg ttgccgatat tttagatcgt cacattagcg aaggttattc
    16861 gttagaagat ttagcaaaac gccaagttat tcaactcaat gatacccatc caacgctcgc
    16921 tatacctgaa ttgatgcgtg tactgttaga tgaacatcaa ttcacttggg agcaggcttg
    16981 ggatatttgc agcaatacct ttgcttacac taaccatacc ttattacctg aagcacttga
    17041 acaatgggat caacgcctat ttaagcagct attacctcgc cattatcaaa ttgtggaaaa
    17101 aatcaatgat attttccacg acaaagtaca ggcagaattt ggtgatgatg caaaaatttg
    17161 ggaaaaatta gccgtgctat ttgattaccg tgtgcgtatg gcaaacctct gcgttgtcac
    17221 ctgcttccgt gtaaacggcg ttgcggaaat tcactccgat ctcttagtga ccgatctatt
    17281 cccggaatac cacaaattat tcccaacaaa attctgcaat gttactaacg gcatcacacc
    17341 tcgccgctgg attcgacaag ctaatccaca attaagtgat ttattggatc gtaccttaaa
    17401 acaagattgg acacacaatc tggaattatt aaaaggcgtt gaacaatatg cggatgacac
    17461 cggtttccgt gaagaatatc aacgcattaa acgcaataac aaagtggttt tagccaatga
    17521 aatccataaa acattagggt taaatgtcaa tcctaatgcg atttttgatg tgcaaattaa
    17581 gcgtttccac gaatataaac gccaacattt gaatttgtta aacattattg cgacctatca
    17641 atcgctaaaa gctaacccga accaagacta cacaccacgt ctattcctat tctcgggcaa
    17701 agctgcaccg ggttattatt tagcaaaaaa tattattcac gctattaata atgtggcaga
    17761 tattattaat aacgacaagc aggtaaatga tcgactacaa gtggcattct taccggatta
    17821 tcgagtcagt cttgcggaaa aaattattcc agcagctgat gtatcagaac agatttcaat
    17881 ggcaggtaaa gaagcctccg gtaccggtaa tatgaaattg gcactcaacg gcgcattaac
    17941 cttaggtacg ctagacggtg ccaatgttga aattgctgat atggtcggtg aggagaatgt
    18001 ctttatcttc ggtcacaccg tagaaagcgt gagggaatta ttagcaaaag gctaccaacc
    18061 gaaagcctac tataaccaag atccgattct gaaaaatgcg gttgatttct tagccacagg
    18121 aaaagcctct aacggagata aagaggcatt caagctgatg ttagatagcc tgttggaacg
    18181 agatcctttc ttagtctttg ccgactttga tagctatcgc caagcacaag agaaaattgg
    18241 tgtagcctat ttagatcaag aaacttggct gcgtagtgca attttaaata ctgctcgttt
    18301 gggtgcattt agctcagacc gctcaattaa agattatcaa caacgtattt ggttgaatag
    18361 ataataaatt cgggcgactt ctacaataaa ggaagtcgcc tattttctgc tgtatttcat
    18421 atgtatttat atatggagag aaatgatgaa aataactgcc aaacaatatt atcgggctgg
    18481 aattatgccc tattttttcg atgagcgtgg tgtaaaaaaa acagcatctg aaaaaataaa
    18541 acattcgtta ttcaaagcct ttaacggcaa tactcaagca agagaaacac ctatccctac
    18601 ggtcaacatt ttttaccagc accaaccctg ttttttacct tttcatttag cggataaaaa
    18661 acatccgtta accggtactt ggcagctaca actagaaaat tcaaaccaaa ttatttccgg
    18721 caaaattaaa gcaaggggaa ttacattacc tcaagatttg cccttaggtt atcaccaatt
    18781 acagctaaaa acggtagata aaacctttaa ttgcaccatt attatcgctc cggaacgctg
    18841 ctaccaacca caagccgtca ttgaacaaaa aagattgtgg ggaaccttcc tacaactata
    18901 caccttaaaa tcagcccaca actgggggat tggcgatttt ggcgatttaa aagacttcat
    18961 tcaacagatt gccccttacc aagcagattt tatcggcttg aacccgattc actcactttt
    19021 tccggcaaac ccgaattctg ccagcccata cagtccatcc tctcgccgat ggctgaatat
    19081 tgcttacatt gatatcaatc agctagcgga atttcaacaa agtgatgaag cacaaacttg
    19141 gtttcattct gctgaaatcc aacaacaatt atcagaatta cgcactcagg attggttgaa
    19201 ttacgagcaa atcattccgt tgaaattgaa agggctacgc tttgctttcg caaaatttca
    19261 gcaaaatatg accgcttgcg ctgcccctgc ttttatcgaa tttgtacaaa aaggtggtga
    19321 aagcttacaa gttcaagcca catttgatgc ccttcatcag catctatcgg cacaatttgc
    19381 cgaccaatgg ggctgggatt tctgggagaa agcctaccaa gactaccact ccgcagccgt
    19441 gcagcaattt agagaaacgc ataaaaccga catagaattt tacctttggt tacaattcgt
    19501 tgccgatcaa cagctagcag aatgtgatgc tatctgccgt cagcaaaata tgcgtattgg
    19561 tatgtatcgc gatctcgccg ttggcgtcag cccgaatggt gcagaaacgt ggaatgacaa
    19621 acaactttat tgcttaaatg cctctgtcgg cgctccgcct gatgtattag gtccgcaagg
    19681 acaaaactgg ggcttaaccc caatgaatcc gcatgtatta aagcaacaag cctaccgtcc
    19741 gtttattgag ttagtcagag ctaatatgag tcattgcggt gcattacgcc ttgaccatat
    19801 tatgtcttta ctccgtttat ggtggatccc caaaggcgat agtgcggtta atggtgctta
    19861 tattcgttat ccagttgatg atttaatcgc aattttggca cttgaaagcc aacgccacca
    19921 atgcctaatt atcggtgaag atttaggtac tgtgccaaaa gaaattgtga gcaagctcaa
    19981 aaatgcgggg attttatctt acaaaatttt ctattttgaa tttgatgaac aagggaagag
    20041 tcgagactta cagcattacc cttaccaagc aatgaccaca ttaagcaccc acgatttacc
    20101 gactatcaat ggttattggc gtggctatga ttttgaatta ggcgaaaaat tcggcgtcta
    20161 tcctaattcg aatattttgg caatccttaa gcaagatcga gtcaatgcca aagccaaaat
    20221 cttacaacgc ttacaagaac ataacgtaga gatcgcacct gaaattagcg agcaacttaa
    20281 ttctccggtg aataccgcat ttattcactg tttacaaacc tatgttgcaa atgtaagcag
    20341 cggattattc ggcttccaac ccgaagactg gttgggtatg actgagccgg ttaacattcc
    20401 gggaaccagt acacaatatg caaactggcg acgtcggctt agtgttaacc tcgaggagat
    20461 cttcacagat aaag
//
LOCUS       DANLUQ010000026        20411 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.026, whole genome shotgun sequence.
ACCESSION   DANLUQ010000026 DANLUQ010000000
VERSION     DANLUQ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 20411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20411
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.026"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..254)
                     /locus_tag="PXB27_000778"
     CDS             complement(<1..254)
                     /locus_tag="PXB27_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HDL5345597.1"
                     /translation="MPTKPHKEYHELVELLISRGMLINDKSYAEKKLSQVGYYRLSGF
                     WHISRKPNQDGGLSENFLENVQFEEVYKLYIFDKKLRLLL"
     gene            complement(466..2583)
                     /gene="spoT"
                     /locus_tag="PXB27_000779"
     CDS             complement(466..2583)
                     /gene="spoT"
                     /locus_tag="PXB27_000779"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HDL5345598.1"
                     /translation="MYLFEPLDRIIQGYLPPEQIERVKRAYVIARDAHEGQTRSSGEP
                     YITHPVAVASIIAEMKLDQEAVIAALLHDVIEDTPYTEEELAAEFGQSVADIVQGVSK
                     LDKLKFRTRQEAEVANFRKMILAMTKDIRVVLIKLADRTHNMRTLGALRPDKRRRIAK
                     ETLEIYSPLAHRLGIEHIKNELEDLGFEAMHPQRYRVLKMAIASARGHRKELIQRIAD
                     DIRGRLEEVQIEARVYGREKHLYSIYQKMRQKDQHFHSILDIYAFRVVVDNVDNCYRV
                     LGQMHALYKPRPKRVKDYIAVPKPNGYQSLHTSMIGPHGVPVEVQIRTEDMDKMADMG
                     VTAHWRYKEGESHHNTTVQLKAQQWLQSIVELQNSAGNTAEFIDSVKSDLFSDDIFVF
                     TPKGRIVELPANATAIDFAYAVHSGIGDRCVGAMVDRNPYPLSEPLRTGQTVEIITEQ
                     GKRPNASWLNFVVSGKARAKIRQSLKNQQHDEAIALGRRQLVRAMNLRSLDELCSETI
                     QSVLDELRLSSFDELLAEIGLGNQMSNVIAQRLLGEPLLIDTDGNPENNQPLKIEGIE
                     NRLISFANCCLPIPGDEIIAHVSSGKGLAIHNACCGNVKDYATEPSNYLPVEWEIAKE
                     KAIQFETEIVLDIINEPGVLAGITSTLAKLNSNIGSINSEAREGNIYQVRVQISVENK
                     EHSELILRKLLSVKGVVKAARKQ"
     gene            complement(2693..2974)
                     /gene="rpoZ"
                     /locus_tag="PXB27_000780"
     CDS             complement(2693..2974)
                     /gene="rpoZ"
                     /locus_tag="PXB27_000780"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL5345599.1"
                     /translation="MARVTVQEAAEKIGNRFDLILIAARRARQLQLHTREPLVPEEND
                     KPTVIALREIEEGLITTDIMDQLENHDSIKQEAAEQEAISFLTEMSNEA"
     gene            complement(3077..3574)
                     /locus_tag="PXB27_000781"
     CDS             complement(3077..3574)
                     /locus_tag="PXB27_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL5345600.1"
                     /translation="MAHFYEYLEFNSDEDRENQLEVYVEVKLSTDTEEKLQAVGVQGD
                     WLVMAEPYCPDCVEIVAYFQRMAKLNPNIKVKYVARKDNQERKHFDSDEQQQAVISAQ
                     KIPTIFDIRNGKTELVLSEFPQFLKDKMAANPEQFEQLKADFRMGKFGKEVEAELLAI
                     LLNTK"
     gene            complement(3599..4621)
                     /locus_tag="PXB27_000782"
     CDS             complement(3599..4621)
                     /locus_tag="PXB27_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005825060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL5345601.1"
                     /translation="MSVTKKRLTLSDIAELSGVSKTTVSMILNGRGNDFRIKSETCQK
                     VKEIAKKHGYRANVYAKSLQAQRTNVIGLVIPDLTNYGFALTSRTLEKLCRENGLQLV
                     IACSDDTFEGEKKAIERLLDRQVDLLITAPTHNEPTYYQNAAQYTPMLQLDRYISGLN
                     IPSITSNDTQSISMLVENIVRAYHLKEFFYLGGQLSLSPSQNRLKGFREGLEQSHLAL
                     DENWVLHQDYQPESGYQMFAQIVEKLGRLPEAVFTASFTLLEGVMRYLTEHQQMDKLL
                     TQELHLATFDDHHLLNALPFHIHSIKQDHQQIAERTFELIQQKLAQQKIDDVKVDCEI
                     LWRKSI"
     gene            complement(4661..6091)
                     /locus_tag="PXB27_000783"
     CDS             complement(4661..6091)
                     /locus_tag="PXB27_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HDL5345602.1"
                     /translation="MHIFNNGKYKSLHAQSEGELNALRQTVLSDPDFYPTYHLAPETG
                     LFNDPNGLIFDGEKYHIFAQWFPYGALHGMKHWQHFITTDFQTFSKGDLLIPDELFES
                     HGCYSGGAILWQDKIVAFYTGNTRRPSDNARIPHQNIAIFDKSGKLLEKRCIIDQAPA
                     GYTEHVRDPKPYLTAEGKIRFVLGAQRENLTGTALIYEMDDLNATPRLIAELEVHNFD
                     NSNVFMWECPDLFSLGGKDLFVWSPQGKLRESHQFQNNYHATYALGELNGNRLRAEHI
                     EELDYGFDFYAPQTVENSDRILFGWVGLPDLTYPTDKYQWHSILTLPRKLSLQNGKVI
                     QNPIVNVGELQAVEMSENFAIENLDTAYLQISVENQPLVLDFFSNEQGQKLSLRFENG
                     VLSLDRSQTEQTELMEKFGSVRHCKIEQLETIEIFFDRSVVEIFVNGGEKVLTSRFFI
                     AKRENVVKSSRPIQVKVAKVLPISVE"
     gene            complement(6196..7119)
                     /locus_tag="PXB27_000784"
     CDS             complement(6196..7119)
                     /locus_tag="PXB27_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HDL5345603.1"
                     /translation="MSKIWVTGDAVVDLIPDGEHHYLKCAGGAPANVAVGVSRLGVEA
                     GFIGRVGNDPLGKFMREVLQAENVCTKQMILDAQHRTSTVIVGLDNGERSFTFMVNPS
                     ADQFLEIGDLPEFNQGDFLHCCSIALINDPSRSTTIEGIRRVKKAGGYVSFDPNLRES
                     LWKSLDEMKTVVNSVVAMADILKFSEEELTLLTETETLEQATQAITAQYPEKLIIITL
                     GKDGAIYHFNGKSQVVAGKALKPVDTTGAGDAFVSGLLAGLAQTPNWHDETVLVEVIR
                     KANASGALATTAKGAMSALPNKAELDAFLAE"
     gene            complement(7236..8663)
                     /gene="miaB"
                     /locus_tag="PXB27_000785"
     CDS             complement(7236..8663)
                     /gene="miaB"
                     /locus_tag="PXB27_000785"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HDL5345604.1"
                     /translation="MAKLHITTWGCQMNEYDSSKMADLLNSTHGFELTENPEDADVLL
                     LNTCSIREKAQEKVFSQLGRWKNWKKDKPNLIIGVGGCVASQEGEHIRDRAPYVDIVF
                     GPQTLHRLPEMINQIRGGKSSIVDISFPEIEKFDRLPEPKAEGPTAFVSIMEGCNKYC
                     SFCVVPYTRGEEVSRPVDDVLFEIAQLAAQGVREVNLLGQNVNAYRGETFDGGICTFA
                     ELLRLVAAIDGIDRLRYTTSHPIEFTDDIIEVYRDTPELVSFLHLPIQSGSDRVLTMM
                     KRNHTALEYKAIIRKLRAVRPEIQISSDFIVGFPGETAEDFEQTMKVIEQVNFDMSFS
                     FIYSARPGTPAADLPDNVSDDEKKERLSRLQQRINHQAMQFSRAMLGTEQRILVEGPS
                     KKDLMELTGRTETNRVVNFVGTPDMIGKFVDVKITDVYTNSLRGEVIRTEDQMGLRVV
                     ESAESVIARTRKEDDLGVGKYVVNL"
     gene            complement(8880..9380)
                     /gene="crr"
                     /locus_tag="PXB27_000786"
     CDS             complement(8880..9380)
                     /gene="crr"
                     /locus_tag="PXB27_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005702996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL5345605.1"
                     /translation="MGLFDKLFGSKQKESQEIKIYAPLTGEIVNIEDVPDVVFSEKIV
                     GDGVAIRPSGDTIVAPVNGTIGKIFETNHAFSIESEEGVELFVHFGIDTVELKGEGFT
                     RLAQEGQTVKVGEPIIKFDLALLEGKAKSVLTPIVISNMDEISNLSKLNGQVVAGESV
                     VLTLTK"
     gene            complement(9465..11186)
                     /gene="ptsI"
                     /locus_tag="PXB27_000787"
     CDS             complement(9465..11186)
                     /gene="ptsI"
                     /locus_tag="PXB27_000787"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HDL5345606.1"
                     /translation="MITGIAASPGAVFGKALVLKEEPIVLNTRKITESEVEAEKAKFF
                     TGREKAAAQLTAIKEKARRTLGEEKEAIFEGHLMILEDEELEEEILSYLTDNLVTADV
                     AASKVIEMQASMLAEIDDEYLKERAGDIRDIGNRLVRNILNMNIVDLGDIQDEVILVA
                     YDLTPSETAQLNLDKVLGFITDVGGRTSHTSIMARSLELPAIVGTNNVTSLVKTGDFL
                     ILDAVNNEVHLNPSEEKIANFKALQEKLNAEKEELAKLKELPAQTLDGHKVDVAGNIG
                     TIRDVEGVLRNGGEAVGLYRTEFLFMDRTQLPGEEEQFQAYKEIVEAMNGQMVILRTM
                     DIGGDKELPYLDLPKEMNPFLGWRAVRIGFTRREILDTQLRAVLRASAFGNLAVMFPM
                     IISVEEIRLLKSIVAELKDQLRAEGHAFDENLKLGIMVETPSAAVNARHLAKEADFFS
                     IGTNDLTQYTLAVDRGNEIIAHLYNPLSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     VRATALLLGMGLDEFSMSGISVPHVKKLIRSINFADAKALADEALAQPTAADIERLVD
                     EFYQKLN"
     gene            complement(11308..11565)
                     /gene="ptsH"
                     /locus_tag="PXB27_000788"
     CDS             complement(11308..11565)
                     /gene="ptsH"
                     /locus_tag="PXB27_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HDL5345607.1"
                     /translation="MYSKDVVITAPNGLHTRPAAEFVKAAKGFASDITVTSGGKSASA
                     KSLFKLQTLGLTQGTTITISADGEDEQKAVDFLVDLIPTLE"
     gene            11826..13400
                     /gene="thiP"
                     /locus_tag="PXB27_000789"
     CDS             11826..13400
                     /gene="thiP"
                     /locus_tag="PXB27_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease"
                     /protein_id="HDL5345608.1"
                     /translation="MLSTLTKTTAWFIYFSIIALYVFSLWALMDYHQNDTLFVFSDIT
                     ATVAYSLLQASLSAVISLFLGSLLARSFFYLDFKGKNLLYKTISFIWALPSLVIIFAV
                     IGVFGNSGWLAKLFQQLGWNWQFNLYGLHGIILAHCLFNIPFVMKYYTGGLQLTPSSR
                     FQLAAQLNLTGWNYIQIVELPIIKTLLPYMFMTVFLVCFTSFPIVLMLGGSPKYSTLE
                     VAIYQAVTFEFDFAKALILIGVQFVVGIGLQLVMNYISTSALKKLKQQPNRVEIWKPK
                     PTGLAKLGLQAVIFLQSFAIILPLVSVIWAGLSVSKLSERLLNPTLWQATQYSLYLSL
                     IASIFVITISYLIALEARQLLYTKAKLRYSILTAITLFPLLLPIFLLSVGLFILLMNS
                     ELSSEALLLLVGICNGLTLLPFIYPMLFSAMWNSLTSHDKLAQGLGLKGLRRWWIVEK
                     NQLIRPLVTVFALAMSSSLGSFTVIAFFGNADFSSLPYLLYQQLGSYRTEDAAVTALI
                     LMLFALLPFLFSKDKL"
     gene            13397..14047
                     /gene="thiQ"
                     /locus_tag="PXB27_000790"
     CDS             13397..14047
                     /gene="thiQ"
                     /locus_tag="PXB27_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein"
                     /protein_id="HDL5345609.1"
                     /translation="MIELDLHFTYEQMNMHFQLSVKKGERIAVVGESGAGKSTLLNLI
                     AGFEKAESQQSQLWLNQKNHLDTEVAQRPVSMLFQDNNIFPHLTVEQNIGLALVPSLS
                     LNPKQKAQVKQIATQMGIETFLQRQADQLSGGQKQRVALARTLLRDKPILLLDEPFSA
                     LDPQRRAELQQIVFDICQSRNLTLLMVTHQLSESLFLFNRVITVADGEIVSDLSPL"
     gene            14354..15768
                     /locus_tag="PXB27_000791"
                     /pseudo
     CDS             14354..15768
                     /locus_tag="PXB27_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605131.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sucrose transporter subunit IIBC"
     gene            complement(15884..16687)
                     /gene="map"
                     /locus_tag="PXB27_000792"
     CDS             complement(15884..16687)
                     /gene="map"
                     /locus_tag="PXB27_000792"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDL5345610.1"
                     /translation="MNNIPLRNEEEIQKLRVACKLASDVLVMIAPYVKEGVTTGELDK
                     ICHEYILANGATSASLGYHGFPKSVCISLNEVVCHGIPSDDKKLKKGDILNIDVTVIK
                     DGYYGDNSMMYVVGEPSVRDKRLMAVTLESLYVGIRTVKAGVRLKEIGTAIQKYVEKE
                     GFSVVREYCGHGIGTEFHCDPQVVHYATDDGGVVLKEGMVFTIEPMVNAGKKEVRLMG
                     DGWTVKTKDRSHSAQYEHQIVVTKDGCEVLTIRDEEIAEGRISRIMHNS"
     gene            complement(16773..17438)
                     /locus_tag="PXB27_000793"
     CDS             complement(16773..17438)
                     /locus_tag="PXB27_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HDL5345611.1"
                     /translation="MNFTTYFSDENKRRSLILLSLFHIFIIAISNYLVQISFEVQVPL
                     TEFSFPTTWGTLTFPFIFLATDLTVRVFGADLARKIIFVVMFPALVISYVISVLFFET
                     QFQGFSALTEFNFFVFRIALASFAAYLVGQLLDVLVFNSLRQGKRWWLAPTCSTIFGT
                     LIDTFVFFAIAFYKSSDEYMAEHWFTIGSVDYAFKLFVSLLLFLPLYGVILNFIMKKL
                     DLK"
     gene            17631..18320
                     /locus_tag="PXB27_000794"
     CDS             17631..18320
                     /locus_tag="PXB27_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HDL5345612.1"
                     /translation="MLQLRKANERGDGSFSWLKSYHTFSFANYYDLKHIHFSHLRVIN
                     EDFIAPNHGFGKHPHDNMEILTYMLKGRVAHEDSMGNKTEVKAGEFQIMSAGTGVFHS
                     EINPDPNETLHLYQIWIIPNQKDVTPRYNQDRFAPKEGATLILSPNAEEGSFKIYQDM
                     KLWRYQYPTAKSEQLALEPNRSYWLQVVKGNLNINGVELSTSDALGIRQEKALEINTQ
                     DDVEFLLFDLV"
     gene            complement(18388..19179)
                     /locus_tag="PXB27_000795"
     CDS             complement(18388..19179)
                     /locus_tag="PXB27_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HDL5345613.1"
                     /translation="MNNLRIKMISPEIALAKYNELDVLEINHSKVKAKIALQGAQLLS
                     WQPKNTQQDVLWLSEIEPFQTGVAIRGGIPICHPWFGTDKKPAHGTARIQAWNLLEHT
                     SSADGIRVVLGLENQAKLEMILGEVCELYFTHLAPEPAQAALHTYFQVGDITKTEVQG
                     LATQCFDKLTNQEIEVPSPRKISENVDCIYPAQAVNFIQDFANQRTIKVEHINATETV
                     LWNPWHNTVSAMTETAYQNMVCVETARINTLLQQNETLGVRILVR"
     gene            19303..20307
                     /gene="fbp"
                     /locus_tag="PXB27_000796"
     CDS             19303..20307
                     /gene="fbp"
                     /locus_tag="PXB27_000796"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HDL5345614.1"
                     /translation="MKTLGEFIIEKQAEYPGAKGELSGILSSIRLAAKIIHREINRAG
                     LSQDILGTAGSENVQGEAQMKLDVFANETMKKALLAREDVAGFASEEDDNFVAFDNER
                     GRNAKYILMTDPLDGSSNIEVNVSVGTIFSIYRRVSPIGTPVTQEDFLQEGRKQVAAG
                     YVTYGSSTMLVYTTGNGVNGFTYDPSLGLFILSHPDLKTPTEGKYYSINEGQYVTFPQ
                     GVKRFIKYCQETDEAIKRPYSSRYIGSLVSDFHRNLLKGGIYIYPTSTVYPKGKLRLL
                     YECNPIAFLAEQAGGMATDGINPILDIKPTELHQRVPFFVGSTSMVKQAGEFMQEFAK
                     "
BASE COUNT         5899 a         4473 c         3817 g         6222 t
ORIGIN      
        1 aataataaaa gccttagttt tttatcaaaa atatatagct tataaacttc ttcaaattgt
       61 acattttcga gaaaattttc gctaagccca ccatcttgat taggctttct agatatatgc
      121 caaaatccag ataaacgata ataccctacc tgagataatt ttttctctgc atagctttta
      181 tcatttatga gcattcctct agatatgagt agttctacta attcgtgata ctctttatgt
      241 ggttttgtag gcataaaatt gaaggcattt taagaaaggc aaggacgtag cttgacactc
      301 taagtcgatt acatttagta aacctcaggg acaggggcta cgccacgatt gcaagggatt
      361 atacatttta gctgattaaa gtcaaaaggt ttcatcaatt aataatagca atactgtact
      421 tttaaggact ataattgcaa aaaactgata aaattttacc gcttgttact gcttacgggc
      481 cgcttttaca acacctttta cacttaatag cttacggaga atcagctcag aatgctcttt
      541 attttccaca gaaatttgta ctctcacttg atagatattt ccttctcttg cctcgctatt
      601 aatgctgcca atgttgctat ttaatttggc aagcgttgat gtgatccccg cgagtacccc
      661 tggttcattg ataatatcca gtacgatttc cgtttcaaat tgaatcgctt tttctttggc
      721 aatttcccat tccacaggta aataattact cggctctgtt gcgtaatctt tcacattacc
      781 acaacaagca ttgtggattg ccaatccttt acctgaactg acgtgagcaa taatttcatc
      841 acccggtata ggtaagcaac agtttgcaaa actgataagg cggttctcaa tcccttcaat
      901 tttaagcggt tgattatttt ctggattacc atcagtatca attaatagtg gctcccctaa
      961 taagcgttga gcgataacat tactcatttg gttacctaga ccaatttcag ccagtagctc
     1021 atcaaagctt gataaacgta gctcgtctaa tacggattga atagtttctg aacacaattc
     1081 atcaagtgag cgtaaattca ttgcccgaac tagctgtcta cgcccaagtg caatcgcctc
     1141 atcgtgctgc tgatttttaa gcgattgacg aattttagcc ctcgccttac cacttactac
     1201 aaaatttaac cacgatgcat ttgggcgctt accttgctcg gtaataattt ccaccgtctg
     1261 tcctgttcgt agcggttctg aaagtggata cggattgcga tcaaccatcg cacctacaca
     1321 acgatcccca atgcctgaat gcaccgcata ggcaaagtca atagcggtag cattcgccgg
     1381 caattcaaca atacgccctt tcggggtaaa gacgaaaata tcgtcagaga acagatccga
     1441 tttaacgcta tcaataaatt ctgctgtatt gccggcactg ttttgcaatt caacaatact
     1501 ttgtagccat tgctgggctt tgagttgaac ggtcgtgtta tggtgcgact cgccctcttt
     1561 gtaacgccag tgagctgtta cccccatatc cgccattttg tccatatctt cggtgcgaat
     1621 ctgtacctcc accggtacac cgtgcgggcc aatcatcgaa gtgtgtaaag attgatagcc
     1681 gttcggtttt ggcacggcaa tataatcttt cacccgtttc ggacgaggct tatacagtgc
     1741 gtgcatctgc cctaatacac ggtaacaatt atctacatta tcaaccacta ctcggaaagc
     1801 ataaatatct aaaatcgaat gaaaatgttg atctttctgg cgcatttttt ggtaaatgga
     1861 atagagatgt ttttcacgcc catacacacg ggcttcaatt tgcacctctt ctaaccgacc
     1921 acgaatatca tcggcaatgc gttgaattag ctctttacga tgtcctctgg cactggcaat
     1981 agccattttc agtacccgat aacgttgcgg gtgcattgcc tcgaagccta aatcttccaa
     2041 ctcgttttta atatgctcta ttcccaagcg atgtgccaac gggctataaa tctctaaggt
     2101 ttctttcgct atacggcggc gtttatccgg acgcaaagca ccaagcgtac gcatattatg
     2161 ggttcggtcg gcaagtttaa ttaacacaac ccgaatgtct ttggtcattg ccaaaatcat
     2221 tttacggaag tttgccactt ctgcctcttg gcgggtgcgg aattttaatt tatccagctt
     2281 agaaacgcct tgtacaatat ctgctacgct ctgtccaaat tccgccgcca gttcttcttc
     2341 ggtgtaaggt gtatcttcaa tcacatcgtg caacaaagcc gcaatcaccg cttcttgatc
     2401 gagcttcatt tccgcaataa tcgacgccac tgctaccggg tgagtaatat agggctcacc
     2461 gctagagcgt gtttgtccct cgtgagcatc acgggctatc acataagcac gttttacacg
     2521 ctcaatctgc tcgggtggca aatagccttg aataattcga tctaaaggtt caaaaagata
     2581 cacgttatac ctcaacaaca gaaataaaaa tggacaacga atttgcaaat tttgaggaaa
     2641 atcctaccgc ttgcagactc aattgtccat tctctattta cgaaaaataa aattacgctt
     2701 cgttgctcat ttcagttaaa aatgaaatcg cctcttgctc cgccgcttct tgcttaatag
     2761 agtcgtgatt ttccagctga tccataatgt cagttgtaat taaaccttct tcaatttcac
     2821 gtaatgcaat cacggtcggc ttatcgtttt cttcaggcac aagtggctca cgggtgtgaa
     2881 gttgtaattg tcttgcacga cgtgcagcaa ttaagattaa atcaaaacgg ttgccaattt
     2941 tctccgctgc ttcttgtacg gttacacgag ccataaattt actccaaaga tttattcggt
     3001 tttattcata aaaagattgc gaattataca gaaattccgc caattccgca atgaaagcgg
     3061 gctaaattca caaaatttat ttggtattca gtaaaatcgc caataattca gcctcaacct
     3121 ctttgccgaa ttttcccatt ctaaaatcgg cttttagttg ctcgaattgc tcagggttcg
     3181 ctgccatttt atcttttaaa aattgcggaa attcagataa cactaattct gttttgccat
     3241 tacgaatatc aaaaatagtt ggaatttttt gcgcagaaat gaccgcttgt tgctgttcat
     3301 cgctgtcaaa atgtttgcgt tcctgattat ctttgcgagc cacatatttc accttaatgt
     3361 taggattgag ctttgccatt cgctggaaat aagccacaat ttccacacaa tcggggcaat
     3421 atggctctgc cattaccagc cagtcccctt gcacgccaac agcttgcagt ttttcttccg
     3481 tatctgtgga aagcttgacc tccacataca cttctaattg attttcacga tcttcatcgc
     3541 tgttaaattc taaatattcg taaaaatgtg ccataatttt gtcttttcct atctcatctt
     3601 aaatggattt acgccataaa atttcacaat caactttaac gtcatctatt ttctgttgcg
     3661 ctaacttttg ctgaattaat tcaaaggtac gttcggcaat ttgttggtgg tcttgcttaa
     3721 ttgaatgaat atgaaaaggt agtgcattca gtaaatggtg atcatcaaag gttgctaaat
     3781 gcagttcttg agtcaataat ttatccattt gttggtgttc agtcagatag cgcattactc
     3841 cctcaagtag ggtaaaagag gctgtgaata ccgcttctgg gagcctgcca agtttttcaa
     3901 ctatctgagc gaacatttga tagcccgatt ccggctgata gtcttgatgc agaacccaat
     3961 tttcatcaag tgccaaatgt gactgctcca aaccttcacg gaagcctttt aaacgatttt
     4021 gacttggcga gagtgaaagt tgtccaccta agtaaaaaaa ctcttttaag tgataagccc
     4081 gtacaatatt ttccaccaac atactgatac tttgtgtatc attactggtg atagaaggga
     4141 tattcaaccc agaaatatag cggtctagct gtaacattgg ggtatattgt gccgcatttt
     4201 gataataagt cggctcatta tgtgtcggag cagtaatcag taaatctacc tgtctgtcta
     4261 ataaacgctc tatcgctttc ttctcacctt caaaggtatc atccgagcaa gcgatcacta
     4321 gctgtaaccc gttttcacgg cataatttct ctaaggttct tgaggtcaga gcaaagccat
     4381 aattcgttaa atcgggaatg actaaaccga tcacattcgt gcgttgagcc tgtaatgatt
     4441 ttgcatatac attagcacga taaccgtgtt ttttagcgat ctctttcact ttttggcagg
     4501 tttcggattt gattctaaaa tcattgcccc gcccatttaa aatcatactg actgttgttt
     4561 tagatacgcc gctgagttcc gcaatatcac tgagcgttag gcgtttttta gtcacactta
     4621 tctcctttta gcataaaaat gggcattact gcccattgct ttattctacg ctaattggca
     4681 atacttttgc tactttcact tgaatcggac gagagctttt tacgacattt tcacgcttag
     4741 cgataaaaaa gcgtgaagtc agcacttttt ccccgccgtt tacgaaaatt tccaccacag
     4801 aacggtcaaa gaaaatctcg attgtctcta attgttcaat tttgcaatga cgaaccgagc
     4861 caaatttttc catcaattct gtttgttcgg tttggctacg atctaagctc aacaccccat
     4921 tttcaaaacg taagctgagc ttttgcccct gttcattgct gaaaaaatcc aacacaagcg
     4981 gttgattttc cacagaaatt tgcaagtagg cagtatccaa attctcgatt gcaaaatttt
     5041 ctgacatttc gaccgcttgt aattcgccta cattcacgat tgggttttgt atcactttgc
     5101 cgttttgcag ggaaagttta cgaggcaatg tcagtattga atgccactga tatttatcag
     5161 ttgggtaagt gagatcaggc aagccgaccc agccaaacaa aatgcgatcg gaattttcca
     5221 ccgtttgcgg tgcgtaaaag tcaaagccgt agtccagctc ttcaatatgc tctgcccgta
     5281 atctgttgcc gtttagctcg ccaagtgcgt aagtggcgtg gtagttattt tggaattgat
     5341 ggctttcacg cagtttgcct tgtggcgacc acacaaacag atctttacca ccaagtgaga
     5401 acagatccgg acactcccac ataaacacat tgctgttatc aaaattgtgg acttccagtt
     5461 ctgcgattaa acggggtgtg gcatttaaat cgtccatttc ataaattaac gccgtgccgg
     5521 tcaagttttc acgctgagcc cccaacacaa aacgaatttt gccctctgcg gtaaggtaag
     5581 gtttcgggtc acgcacgtgt tcggtgtagc ctgccggtgc ttggtcgata atgcaacgct
     5641 tttccaataa tttgcccgat ttatcaaaaa tcgcgatatt ttggtgagga atgcgggcat
     5701 tatctgatgg gcggcgtgta ttgccggtgt aaaaagcgac aatcttatct tgccataaaa
     5761 tcgccccacc tgaataacag ccgtgggatt caaacagctc atcgggaatc agcaaatccc
     5821 ctttactgaa cgtttgaaaa tcggtagtga tgaaatgctg ccaatgtttc attccatgta
     5881 atgccccata aggaaaccat tgggcgaaaa tatggtattt ttcaccatcg aaaatcaagc
     5941 cgtttgggtc attaaataag cctgtttcgg gagcgagatg ataggttggg taaaaatcgg
     6001 gatcagataa aacggtttgg cgaagagcgt ttaattcgcc ctcgctttgt gcgtgcaggc
     6061 ttttgtattt gccgttgttg aaaatgtgca tagagtgttc ctgtcatttc agaaattacg
     6121 atggtgcaag cgtccacgct tgtacccttt tgtaaaaaat gaggcacaag cgtggacact
     6181 tgcgccatca ttacattatt ccgccaaaaa tgcgtctaac tccgctttat tcggtaatgc
     6241 cgacatcgcc ccttttgctg tggtggctaa cgcaccggaa gcatttgctt tgcggatcac
     6301 ttcaactaac acagtttcat cgtgccaatt cggcgtttga gcaagaccgg ctaataatcc
     6361 gctgacaaag gcatctccgg caccggtggt atccacaggt ttcaaggctt tgccggcaac
     6421 gacttggctt tttccgttga aatggtagat tgcaccgtct ttgccaaggg tgataataat
     6481 caatttctcc ggatactgag cggtaatagc ttgtgtggct tgttccaaag tttctgtctc
     6541 ggttaataag gtcagctctt cttcggaaaa tttgagaata tccgccatcg ccaccacaga
     6601 atttaccact gttttcattt catcaaggct cttccagagc gattcacgca agtttggatc
     6661 aaacgaaaca tagccacccg cctttttcac tcggcgaatc ccttcaatgg tagtggaacg
     6721 ggatgggtcg ttaatcaagg caatagaaca gcagtgtaag aaatcacctt ggttaaattc
     6781 aggtaaatcg ccgatttcta aaaattggtc ggcactcgga tttaccataa aagtaaaact
     6841 acgctcaccg ttatctaagc caacaatcac ggtggaagtg cggtgctgag cgtctaaaat
     6901 catctgtttg gtgcaaacat tttctgcctg taacacttct cgcataaatt tacccagagg
     6961 gtcgttaccc acacgcccga taaatcctgc ctcaacccct aaacgagaaa cgcctactgc
     7021 aacattcgcc ggagcaccac cggcacattt taaatagtga tgttcgccgt cgggaattaa
     7081 atccaccacc gcatcgcctg ttacccaaat cttactcatc tcatacacct ttaattagtc
     7141 acaaaacacc aagctaaaac gatttagcat ttattcgatt atatactaaa agaccgcttg
     7201 tacataccta caagcggtca ttttttcttt aatttttaca gattaaccac gtatttacct
     7261 acacctaaat catcttcttt acgagtgcgg gcaatcacgc tttctgccga ttccaccaca
     7321 cgtaatccca tttggtcttc ggtacgaatc acttcgccac ggagtgagtt ggtataaaca
     7381 tcggtgattt tcacatcgac aaatttgcca atcatatccg gtgtgccaac aaagtttact
     7441 acacggttag tttcggttct gccggttaat tccatcaagt ctttcttaga cgggccttca
     7501 accaaaatgc gttgttctgt accgagcatt gcacggctaa attgcattgc ttggtggttg
     7561 atgcgttgtt gtaaacggga taatcgctct tttttctcat catcggagac attatcaggc
     7621 agatctgccg ctggtgtacc cggtcgagcc gagtagataa agctaaagct catatcaaag
     7681 ttcacttgct cgatcacttt cattgtttgc tcgaaatcct ctgcggtttc gcccgggaag
     7741 cctacaataa agtccgaact aatttgaatc tcaggtcgaa cagcacgcaa tttacggata
     7801 atcgctttgt attccaacgc tgtgtggtta cgcttcatca ttgttaatac acggtctgaa
     7861 ccactttgaa tcggtaaatg taagaaactg accaattcag gtgtatcacg ataaacttcg
     7921 ataatatcat cggtaaattc aatcgggtgg ctggtggtgt aacgtaagcg gtcaataccg
     7981 tcaatcgctg ccactaagcg taataattcg gcaaaggtac aaatgccgcc atcaaaagtt
     8041 tcaccacggt aagcatttac gttttgaccg agtaaattaa cctcacgcac accttgtgcc
     8101 gcaagttggg cgatttcaaa taatacgtca tccaccgggc gggaaacctc ttcgccacga
     8161 gtgtaaggca caacgcagaa cgaacaatat ttattgcagc cttccataat tgaaacgaat
     8221 gctgtagggc cttcggcttt tggctccggc aagcggtcaa atttttcaat ttccgggaaa
     8281 gaaatatcga caatcgagct tttaccgcca cggatttggt tgatcatttc aggcaaacgg
     8341 tgcaaagttt gtggcccgaa aacaatatct acataagggg cacgatcacg gatatgctcc
     8401 ccttcttgtg aagccacaca accgccaaca ccgataatca ggttcggttt atcttttttc
     8461 cagtttttcc aacgcccgag ttgcgagaat actttttctt gtgctttttc acggatagaa
     8521 caagtgttta gcagcaacac gtctgcatct tcagggtttt cagtcagttc aaaaccgtga
     8581 gtagagttta ggagatccgc catttttgat gagtcgtact cattcatttg gcagccccaa
     8641 gtggtaatat gtaatttagc cataacgttt aaaattaata aataattcaa taacttagaa
     8701 aagaaaagcg taaagcaatt tattattttt gacttaaaat aaacccttta cgcaaaattt
     8761 gtggcgttat tttaccttgt tgcctatttt ttatctataa ataatcgcca tttatccgat
     8821 tttacctaaa acaaaaaagc cacgatcttc catcgtggct tccagctaag taggaggtat
     8881 tatttggtta aggttaaaac cacgctttca ccggcaacaa cttggccatt taatttagat
     8941 aagttgctga tttcatccat atttgaaatc acgattggtg ttaatactga tttggctttg
     9001 ccttcaagta gagctaaatc aaatttaatg atcggctcgc ccacttttac ggtttgacct
     9061 tcctgtgcaa ggcgagtaaa gccttcgcct tttaattcaa cagtatcaat accgaagtga
     9121 acaaataatt ccacgccttc ttcagattca atcgaaaaag cgtggttggt ttcaaaaatt
     9181 ttgccgatag tcccatttac tggagcaacg atagtgtctc cactcgggcg aattgccaca
     9241 ccgtcaccta caattttttc agagaaaaca acatcaggca catcttcaat atttacgatt
     9301 tcacctgtta atggcgcata gattttgatt tcttgactct ctttttgttt tgagccgaat
     9361 aatttatcaa ataaacccat cttaaaatct cccttagata ttagatacaa aattctcgta
     9421 cattttatac gagaattttg tatttgtatg ctaaataatt aaaattagtt taatttctga
     9481 taaaactcat ctactaagcg ttcaatatct gcagcagttg gctgagcaag cgcctcatca
     9541 gctaatgctt tagcatcggc aaaattaatt gagcgaatga gttttttaac gtgtggcaca
     9601 gagataccgc tcatactaaa ttcatctaat cccataccga gcaacaacgc agttgcacgc
     9661 acgtcaccgg ctaattcacc acacatacct gtccatttgc cttcagcgtg tgaagcatca
     9721 ataacttgct tgattaagtt taagactgat gggcttaatg ggttatataa atgagcaata
     9781 atttcattac cacgatctac cgcaagtgta tattgggtta agtcattagt accaatacta
     9841 aagaagtcgg cttcttttgc aagatgacgt gcatttaccg ctgctgatgg ggtttccacc
     9901 ataataccta gttttaagtt ctcatcaaaa gcgtgacctt ctgcacgtaa ttgatctttc
     9961 aattccgcaa cgatggattt taataaacgg atttcttcca ccgaaataat catcgggaac
    10021 attaccgcta aattaccgaa cgcagaagca cgtaataccg cacgtaactg agtatctaaa
    10081 atttcacggc gggtgaaacc gatacgcacc gcgcgccaac ctaagaatgg gttcatttct
    10141 ttcggtaaat caaggtatgg taactcttta tcacctccga tatccatcgt acgcagaatg
    10201 accatttgac cattcattgc ttcaacaatc tctttgtagg cttgaaattg ctcttcttct
    10261 cccggtaatt gagtacgatc cataaacaaa aactcagtac ggtataaacc aaccgcttca
    10321 ccaccgttgc gtaatacacc ttccacatca cggatagtac cgatattccc tgccacatca
    10381 actttatggc catctaatgt ttgagcaggc aactctttta atttggctaa ttcttctttt
    10441 tccgcattta atttctcttg taatgctttg aaattagcga ttttttcttc cgatggattt
    10501 aaatgtactt cattgttcac tgcatctaaa atcagaaaat caccagtttt aactagggaa
    10561 gtaacattgt tagtaccaac aatggcagga agctctaggg aacgagccat aatagaagtg
    10621 tgagaagtac gaccaccgac atcggtaata aagcctaata ctttatctaa atttaattga
    10681 gctgtttcgg aaggggttaa gtcataagcg actaaaataa cttcatcttg aatatcacca
    10741 agatctacga tattcatatt taaaatattg cgtaccaagc ggttaccaat atcacgaata
    10801 tcacctgcac gttcttttaa atattcatcg tcaatttccg caagcattga agcctgcatc
    10861 tcaatcactt tacttgctgc aacatccgca gtcacaagat tatcagtaag gtaactcaaa
    10921 atttcttctt caagctcttc atcttctaag atcatcaagt ggccttcgaa aatcgcctct
    10981 ttttcctcac ctaatgtacg gcgagctttt tctttaatcg cggttaattg agcagctgct
    11041 ttttcgcggc ctgtaaagaa ttttgctttt tctgcctcaa cttcgctttc agtaattttg
    11101 cgagtattta gtacaatcgg ctcttctttt aaaaccaatg cttttccaaa tactgcacca
    11161 ggggaagctg cgatacctgt aatcataatt gtgtccttaa tttaagatga tgtaaaaatc
    11221 atctggatgt tacatagcaa aacactttat tgtagcttat taagctacaa taaagtgaca
    11281 taaatcttaa atccgagaat agaaaaatta ttctaaagta ggaatcaaat caactaagaa
    11341 atcaactgct ttttgctcat cttcgccgtc cgcagaaatt gtaattgttg tgccttgagt
    11401 caaacctaat gtttgtaatt tgaataagct ttttgcactt gcacttttac cacctgaagt
    11461 cacagtgata tcagaagcaa aacctttagc tgcttttaca aattcagccg ctgggcgagt
    11521 gtgtaaacca tttggagcgg taataacaac atcttttgag tacataaaat cttcctctta
    11581 aatttgaaag ttaaaaaaat ttgttttaat ccatttattt tgcgcacgca actttactac
    11641 aaattcaaat taaaaacacg ctaaaaagcg caaaaactcg tcgtatcacc ctgctaaata
    11701 gggtgttttt taagctaact taaaaatcat ttgtcgaata tcaagattct agcacacact
    11761 taagcggaaa tgttacaata atttcgctac cacttctttc acttatattc taaatttgga
    11821 gcaatatgct tagcacatta accaaaacaa ccgcatggtt tatctacttt agtattattg
    11881 ctctctatgt ttttagtcta tgggcgttaa tggactatca ccaaaatgat accttatttg
    11941 ttttttccga tataacggcc acggtcgctt atagcctact tcaagccagc ctctctgcgg
    12001 taatttcact atttttaggc agcctactgg caagatcttt cttttatttg gactttaaag
    12061 gaaaaaattt actctataaa accatttcgt ttatctgggc attaccctca ctagtaatta
    12121 tttttgcggt aattggcgta tttggtaact caggctggct agccaaactt ttccaacaac
    12181 tcggttggaa ttggcaattt aatttatacg gcttgcacgg catcattttg gctcactgcc
    12241 tgtttaacat tccgtttgtg atgaaatatt ataccggagg gcttcaattg actccaagca
    12301 gtcgctttca gcttgcggct caattaaacc taacaggttg gaattatatc caaattgtgg
    12361 aactaccgat aatcaaaact ttgctgcctt atatgtttat gactgttttt ctggtctgct
    12421 ttaccagttt tccaatcgta ttaatgttag gcggtagccc aaaatacagc acgcttgaag
    12481 ttgccattta tcaagcggtc acttttgaat ttgattttgc aaaagccctc atcttaattg
    12541 gggtacaatt tgtagtgggc attggcttac aattagtaat gaattatatc tcgacttctg
    12601 cactcaaaaa gctaaaacag caacctaacc gagtagaaat ttggaaacca aagcctaccg
    12661 gactggctaa actcggctta caagcggtta tttttttgca aagttttgca ataattctac
    12721 cgcttgtcag cgtcatttgg gcaggactaa gcgtgtcaaa actcagtgag cgtttgctca
    12781 accctacttt atggcaagct actcagtatt ctttatactt aagtttaatt gcttctatat
    12841 tcgtaatcac aatcagctat ttgattgcac ttgaagctcg ccaattactc tatacaaaag
    12901 ccaagctcag atatagcatt ctcaccgcaa tcaccctatt ccccttactt ctgccgatct
    12961 ttttattatc ggttggtttg tttattttat taatgaatag tgaactctca agcgaggcat
    13021 tactgctact ggtcgggata tgcaacggct taacattact gccatttatt tacccaatgc
    13081 ttttctccgc aatgtggaat agcctcacct cgcacgacaa attagcacaa ggacttgggc
    13141 taaaggggct tcgccgttgg tggattgtgg aaaaaaatca actaattcga ccgcttgtta
    13201 ccgtatttgc gttagcaatg tcatcgagct taggcagttt caccgtcatt gcttttttcg
    13261 gcaacgctga tttcagctca ctcccttatt tactctacca acaacttggt agctatagaa
    13321 ccgaagatgc tgccgttacc gcactcattc tgatgttatt tgcgttacta ccattcttat
    13381 tcagcaagga taaactatga tcgaactgga tctccatttc acttacgaac aaatgaatat
    13441 gcactttcag ctttctgtca aaaaaggcga acgtattgca gtggttggcg aaagtggcgc
    13501 aggcaaaagc acattactta atctaattgc cggttttgaa aaggcagaaa gtcagcaaag
    13561 ccagctgtgg ctaaatcaga aaaatcatct tgataccgaa gttgcacaac gtcctgtttc
    13621 aatgctattc caagataaca atattttccc gcacttaacg gtagaacaaa acatcggctt
    13681 agctttagta ccaagtttgt cgctcaatcc gaaacaaaaa gcacaggtta aacaaattgc
    13741 cacacaaatg gggattgaaa ccttccttca gcgtcaagcc gatcagctat cgggcggaca
    13801 aaaacagcga gtggcattag ctcgtacttt gttgcgggat aagccgattt tattattgga
    13861 cgaacccttc tccgccctcg acccgcaacg ccgagccgaa ttacagcaaa tcgtgtttga
    13921 catttgccaa agcagaaatc taaccctgtt aatggtaact caccaactat ccgaaagcct
    13981 atttctattt aatcgggtga taacggtagc agatggggag attgtttctg atctctcccc
    14041 tctttagcaa agcagatctg ctaggttcta aatgtaggga cgccacgctg gcgtacgcaa
    14101 cgatgtcaat tccaacaatc taacggtgaa tgatgttccg cacggatact agcgttattc
    14161 atttttttca gaactcgccc cctactttag caaagagggg aactgtaact aatattacct
    14221 ccctattccg ctattctctt ccccaaaatg tgatctactt cacaaatttg gaaatatttt
    14281 aaatcttctc ttttttagct aaaccgattt agttaaaatg caaccgatta ttttttatga
    14341 aaggtaaatt caaatgaact atccaaaaat tgcagaacaa acgattgcaa aattaggtgg
    14401 caaagaaaac attaccacac ttgcccactg tgcaacccgt ttacgcttaa ccattgctga
    14461 tgaatccaaa attgataaag aggcgattga aaatattgaa ggcgtgaaag gtcaattctc
    14521 tactcccggt caataccaaa ttatttttgg atcaggcacg gtaaatgaag tgtatgccca
    14581 aatgcaaaaa caaatgggta tggcagatat gtccacctct gaagtagcgg ctgccggtgc
    14641 agcaaatcag ccattactac aacgtttggt aaaaggctta gccgatattt tcgtgccgat
    14701 cattccggct attgtggctg gcggtttgtt aatgggtatt cactcaatgc taacggcagt
    14761 cggtttcttc tgggaaggtg aatctgttgt tagccgttat ccgaatattt ccgatttagt
    14821 tgatttcatc aacactattg ctaacgcacc atttgtattc ttacctgtat tactcggttt
    14881 ctcagctaca cgcaaatttg gcggtaatcc attcttaggt gcggcacttg gtatgttatt
    14941 ggttcacccg gcacttgcag acggttggaa ctatgccaaa accttaatgg aaggtaacat
    15001 caaatactgg aatattttcg gcttagaaat tgaaaaagtg ggttatcaag gcacggttat
    15061 tccaactatc gtttcggctt gggtgttagc aacccttgag aaaactttcc gtaaatttgt
    15121 gccaagctat ttagataact tggtgacgcc aatgatgtca ttatttatcg ccggtatttt
    15181 agctttcact gtaatcggac cattgggtcg tgaagcaggt tcagcgattt cagcaggctt
    15241 aacgtggtta tatgacagct taggctttat cggtggagca attttcggta cattctacgc
    15301 tccaatcgtg attaccggta tgcaccaaac ctttattgcg gttgaaaccc aattattagc
    15361 tgaagtagca caaaccggcg gtacatttat cttcccgatt gcagcaatgt ctaacattgc
    15421 acaaggtgca gcctgtttag gtgtagcttt tgcaatgaaa gatgccaaag tgcgtggttt
    15481 agccgtgccg tcaggtattt cagccctgtt aggtatcaac cggcaatgtt cggagtaaat
    15541 ttacgctacc gccaagcctt tattgcggcg atgatcggtt caggtttagc cagtgcattt
    15601 atcgctttct ttaatgtgaa agcaattgcc ttaggtgcag ccggtttctt aggtattccg
    15661 tcgattaaac ctgacagcct tgcaatgtat tctgtcggta tggtaatttc atttgtagtt
    15721 gcatttacca tttcagccat ttgggtgaaa aaagcacaag cgaaataatc aaaaatagat
    15781 cacaaaaaag ggattaagtg ttagcttaat cccttttgtt tttaaacgcc ttagcttaac
    15841 aatgagtttc gcaagcggtc atttttcgct aaaaatttgc aaattagctg ttgtgcataa
    15901 ttcgagaaat acgcccttcg gcaatctctt catcacgaat tgtcagcact tcacagccgt
    15961 ctttggtaac tacaatttga tgctcatatt gtgcggagtg gctgcggtct ttggttttca
    16021 ctgtccaacc gtcacccatt aagcggactt cttttttgcc ggcattcacc attggctcaa
    16081 tcgtaaacac cattccttct tttaacacta caccgccatc atcggtagca taatgcacta
    16141 cttgtgggtc gcaatggaat tcagtaccaa taccgtgtcc gcaatattct cgtactaccg
    16201 aaaagccttc tttttccaca tatttttgaa tcgctgtacc aatttctttt aaacgcaccc
    16261 ccgctttcac ggtgcgaatc ccgacataaa gggactctag cgtaaccgcc attaagcgtt
    16321 tatcccgcac acttggctca ccgactacat acatcatcga gttatcgcca tagtagccat
    16381 ctttgataac cgtcacatcg atgtttaaaa tatcgccttt tttcagcttt ttgtcatcac
    16441 ttggaatacc gtgacaaacg acctcattta acgaaatgca caccgatttt gggaagccgt
    16501 gatagcccaa actggctgaa gttgccccat ttgccaaaat gtattcgtga caaattttat
    16561 ctaattcacc agttgttacg ccctctttca cataaggggc gatcattact aaaacatctg
    16621 aagccagttt acaggctact cgtagttttt gaatttcttc ttcgtttctt aatggaatat
    16681 tattcattac attgctctct gctatatctg ccataaaaag taaggggatt ataccttttt
    16741 tcagcacaat ccccaaacct cagtttagat ttctacttta aatcaagttt cttcataatg
    16801 aaatttaata tcacaccata aagcggtaga aatagcagta ggctcacaaa gagtttaaac
    16861 gcataatcga ccgaaccaat agtaaaccaa tgctctgcca tatattcatc gctactttta
    16921 taaaaggcga tagcaaagaa tacaaaagta tcgattaaag taccaaaaat ggttgaacag
    16981 gttggagcaa gccaccaacg cttaccttgt cttaaactat taaatactag aacgtctaat
    17041 aattgcccaa ctaaataagc tgcaaagcta gccagtgcaa tacggaaaac aaaaaagttg
    17101 aactcagtta atgcactaaa gccttgaaat tgggtttcaa agaataaaac ggaaatcaca
    17161 tagcttatga caagtgccgg aaacatcaca acaaaaataa tttttctagc aagatctgca
    17221 ccaaatacac gcacggttaa atcggtcgct aagaaaataa atgggaatgt taacgttccc
    17281 caagttgtcg gaaagctaaa ttctgttagt ggtacttgaa cttcaaagct aatttgcact
    17341 aaatagttgc taattgcgat gataaaaata tggaaaagtg aaagtaaaat tagagaacga
    17401 cgtttgttct catccgaaaa ataagtagta aagttcatat tgtatccttt ttcgttaaat
    17461 gaaaacttga ggtaagggaa ctcaaggggg cattattcta tacattttcc cataaaaatc
    17521 aagctcatat tatttcaaaa aatgaaacaa ttaatctatt aaattagggt ttatctattt
    17581 ttgacataaa gtaaaatggc acaacttaac acttacataa ggaatagata atgttacaac
    17641 tgagaaaagc caatgaacga ggcgatggtt cttttagctg gcttaaaagc taccacactt
    17701 tttcctttgc caattattac gacctaaaac atatccattt ttcacattta cgggtaatta
    17761 acgaagattt cattgcacct aatcacggtt ttggcaagca cccgcacgat aatatggaaa
    17821 ttttaactta tatgttaaaa ggacgtgtgg ctcacgaaga cagtatgggt aataaaaccg
    17881 aagtaaaagc tggcgaattt caaattatgt ctgccggaac cggggttttc cattcagaaa
    17941 ttaatccaga tccgaatgaa accttacacc tttaccagat ttggattatc ccaaaccaaa
    18001 aagatgtgac ccctcgctac aaccaagata gatttgcccc gaaagaggga gcaaccttga
    18061 ttctttcgcc aaacgcagaa gaaggttcat ttaaaattta ccaagatatg aagctgtggc
    18121 gttatcaata cccaacagcc aaatcagagc aactggcatt agagccaaac cgcagttact
    18181 ggctacaagt ggtgaaaggg aatctaaaca tcaatggcgt tgaactttcc accagcgatg
    18241 ccttaggcat tcgtcaagag aaagcacttg aaataaacac tcaagatgac gtggaatttt
    18301 tactgtttga tctagtgtaa atacaagaga caagcggtta aatttgtaaa aaatattact
    18361 aattcagctg cttgctctca ttaaacatta tcgcaccaaa attctcactc ctagcgtttc
    18421 attttgttga agtaaggtat ttatccgtgc tgtttccaca cataccatat tttgatacgc
    18481 cgtttctgtc atcgcactaa cggtattatg ccaaggattc caaagtacgg tttcggtagc
    18541 attgatatgc tcaactttaa tggtgcgctg atttgcaaaa tcttgaataa aattcaccgc
    18601 ttgtgccgga taaatacaat ccacattttc acttatcttg cgaggtgatg gcacttcaat
    18661 ttcttgattt gtcaatttat caaaacattg tgttgctaag ccttgtactt ccgttttcgt
    18721 aatatcaccg acctgaaaat aagtatgcaa tgcagcctgt gccggctccg gtgctaagtg
    18781 agtaaaatac aactcacaga cttcgcctag tatcatctcg agttttgcct gattttctaa
    18841 ccccaaaaca acacgaatcc catctgctga ggaagtatgt tccagcaaat tccacgcttg
    18901 aatacgggct gtgccgtgag caggcttttt gtctgtacca aaccaagggt ggcaaatcgg
    18961 aatgccgcca cgaatcgcca cacctgtttg gaaaggttca atttcactta accataatac
    19021 atcttgctga gtatttttag gttgccaact cagtaactgc gcaccttgca atgcaatttt
    19081 agccttcact ttggagtgat taatctctaa aacatccaat tcattatatt ttgctaatgc
    19141 tatttcaggg ctgatcattt tgattcgtag attattcatc gtattgctcc tcatcttgaa
    19201 agataaaatg tgaagtggat cacagaatta aagcttaaac cgctacctct ttaaaggtaa
    19261 tcgttataat aagggcattc taacatagcg aaggagaaaa aaatgaaaac actcggcgaa
    19321 tttattattg aaaaacaagc agaataccca ggggcaaaag gggaattaag tgggatttta
    19381 tcttctattc gtttagccgc taaaattatt catcgagaaa tcaaccgtgc cggtttaagt
    19441 caagatattt taggtactgc aggttctgaa aatgtacagg gtgaagccca aatgaaactt
    19501 gatgtattcg ccaacgaaac gatgaaaaaa gcgttacttg cacgtgaaga tgttgccggc
    19561 tttgcatcgg aagaagatga taattttgtt gcctttgata atgagcgtgg gagaaatgca
    19621 aaatatattt taatgaccga tccgcttgat ggctcctcca atattgaagt gaatgtttct
    19681 gtgggaacaa tcttctctat ttaccgccgt gtctccccta tcggcacccc agtaacacaa
    19741 gaagatttct tgcaggaagg gcgtaaacaa gttgccgccg gttatgttac ttatggttct
    19801 tcaaccatgc tggtttacac cacaggcaat ggcgtgaacg gttttaccta tgatccgtca
    19861 ttaggcttat ttattctttc acaccctgat ctaaaaaccc ctaccgaagg aaaatattac
    19921 tccatcaatg aagggcagta tgttactttc ccacaaggtg tgaaacgctt tattaagtat
    19981 tgccaagaaa ccgatgaagc catcaaacgt ccatactctt cacgctatat cggctcgctg
    20041 gtgtccgatt tccaccgcaa tttattaaaa ggcggtattt atatttaccc aacctccacc
    20101 gtatatccaa aagggaaatt gcgtttactc tatgaatgca acccgattgc gtttcttgcc
    20161 gaacaagccg gaggaatggc gacagatggt attaatccga ttttagatat taagccaaca
    20221 gagctacacc agcgtgtacc gttctttgtc ggttcaacct caatggtcaa acaagcgggc
    20281 gaatttatgc aagaatttgc aaaataatgc tcctgcttat cactcaataa aactttcaac
    20341 aaagcagctg cgtattccgt agctgctttt gcctatctta agattgtaaa tttgtataaa
    20401 aaacaggtaa g
//
LOCUS       DANLUQ010000027        20364 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.027, whole genome shotgun sequence.
ACCESSION   DANLUQ010000027 DANLUQ010000000
VERSION     DANLUQ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 20364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20364
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.027"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            11..694
                     /locus_tag="PXB27_000797"
     CDS             11..694
                     /locus_tag="PXB27_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2572 family protein"
                     /protein_id="HDL5345615.1"
                     /translation="MNKYKNASALLVSLILISTILSVLFLSKEKWLGQQNITHFYREK
                     YLSDQYNFLALYPQDKTQICQKFQKEQISQADLSDKQFRFLQYQFACQFHSLFKDKKP
                     TKEKYIHFTHLADYLDLSNVPNQEIYTIHSLAELPSSSEREPKIVLAKNAINENLPQE
                     FYGIVITDYLFDITGKKMYGTLYSSYDNAREERNLTFKKEVLAELEARYSAWRYLPYS
                     EHLMGAVDD"
     gene            687..980
                     /locus_tag="PXB27_000798"
     CDS             687..980
                     /locus_tag="PXB27_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5374 domain-containing protein"
                     /protein_id="HDL5345616.1"
                     /translation="MIKYKAETALSLLVAITLFAIVVLSFSQWQSAQNHRLNQHFQQQ
                     QAVMILENQLALKLAGLDCESSITQNQIRYQIQCSGNRLTIRFPLGKIELNND"
     gene            1029..4319
                     /gene="recC"
                     /locus_tag="PXB27_000799"
     CDS             1029..4319
                     /gene="recC"
                     /locus_tag="PXB27_000799"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HDL5345617.1"
                     /translation="MFTLYQSNQISSLAEMLVKIQQVNPLEDPFEPETILIQSQGMAQ
                     WLQMQIAELNGVMGNCDFLYPTTFLWQQYRLLFPELPKENIFERSSLVWRIMRLLPSC
                     LTLSEFAPLKRYLGDQPNKEQYQLKLYQLSAKIAELFDQYLVYRPHWLVSWEEGNLQA
                     IENEIKIATAAKPIDFDLIRADMKWQSHLWQQIVSDLQSESDEQVFTTSHRAYLQQNY
                     FAKLDDLTETEKAKLPKRIFIFGISSFSPLQLAVFKKLSEHCDIHLFFFNPSEKYWGD
                     SVEEKVWQKLALSHKISQDDLDNLLAEQRNKLLNLWGKQGRDFLAQLIEFEPNTMDVF
                     VEPEENSNLNKLKKQIFSLENDATITDLTADNSLQIHSCHSPMREVEVLHNQLLNLFE
                     QDSTLLPKDIIVMSPDINKYAPYIDAVFSRYNTGNRDPRYIPFSLSDQAFTQVDPIIH
                     SFLQLLAMKESPFNAEELFDLLEVNAIQTRFGFSTEDIHTLRVWVRNAGVRSGVEIEQ
                     PHWQNYNSWENGLNRLLLGSSLKEEQGIWENTLAFGDSYGLNAELVGRLASFIEALLA
                     WVNFLQTPQPLAAWQQAIKNLTADLYQENAENAASILLLNNVNDQIIEQISATHFSDD
                     LQIEVLALLFEQQLSQHSQQLNFLVGRVNFATLLPMRAIPFKVICLLGMNEADFPRQQ
                     QINSFDLMQYAPKKGDRAKRDDDRYLFLEALLSAQQMFYISYIGQSQKDGKEMLPSIL
                     VSQLLDAMNEALTPECKQQLSDEVEVLVRKQPLSVFSKKNFTHPRDHAYNAEWIIKPT
                     VAVQDFLSEPLTNESVASLELDELISFVQNPIRFFFLKVLGISFNTYDDTIDESEIFE
                     MSGLDNYRILNELLTINLAQQQRFFAEEQLKGNLPASYFAQIATQDLIGKITEMQAAL
                     SHYLASESRILSVNREVELERQTLTLNGNIANLYQNEVVLWRVGHLRDKDKIQTWIYH
                     LLLCAYFPEKTVKFYYREGEQVGVLSFETVSQIEALSQLSIYLNAYLDGLSQAQLVIY
                     DRIEEYLKGDGTNSEQVLIDLAEKDERDGSYLHRLLAQTKELDSQAIHQRTQSWFSLM
                     NQKIAKGD"
     gene            4455..7400
                     /gene="ptrA"
                     /locus_tag="PXB27_000800"
     CDS             4455..7400
                     /gene="ptrA"
                     /locus_tag="PXB27_000800"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HDL5345618.1"
                     /translation="MKLSFLTQFVAAAFALNVMTYPAFAEVAKNSEKTTASQPQSQLG
                     FELIKQSINKSPNDKAIYQGIKLDNGMEVLLISDSQANKSLMSAIIPIGSMDDPISQQ
                     GLAHYLEHMILMGSKNYPETNSLDGFLTKNGGMNNASTASHRTAYYLQVNNEAFSEAV
                     TRLADTLAFPLLLETNAKKEVNAVNAEMVRAKSHDGHLLHSVNLATANPAHPATKFAV
                     GNKETLSDKPDSKLQTELEQFYKQHYSANLFKVVLYSNQSIEQMAKLAANTLGKMENK
                     HFSIPQTAEPRYRDEDKSVFIQYKPVKSNKMLVIGFDFPNDEMQFKHKTGKYIAYMLN
                     NNTDGTLSDYLIKQGLSDGGIEAVSSANIGRDRGSFDIYIELTDKGLSEQDQIISLVF
                     QQIEKIKQEGIQESYFSEVKESLKQDFQHLQVEKNMDFVEFIADQMLHFPLEHVIDQP
                     YLTETMDNEAIKAKLAAMNIDNARIMLVSDTAKTDKKTPYFEAGYSVAKITQEQKQKW
                     LDFSKNPQLNLPELNPYFTTDFTLNKVETGLTKPKLINDQAGNLIYTMPSVYFTNEPK
                     AKIGVGFNIRPRKDDLKSLTAASILGYMNNLAQTKLDFQSSVAGMNLSLAPAANGLAL
                     SAEGYTQNLAKLMLDSVKQFSRFEFNEDVLAQAKQRLHQELDRNEKENSLNQANAALS
                     RFASYPYFDVPKQREMIDSITLADIEQLRQRILQQSSGFKLLSVGNLSAEQVSALAED
                     LQAVIKNQKSANATGQYVDVSTSQRKINVIKSVPNEDNALLMAFMPNGYNELESLTRA
                     KLLRSIISRWYFDDLRTDKQLGYVVYATDNIIGKTAGIQFMVQSPNTTPAGILEHNER
                     FFTQSFERLKNLSDEEFGKYRDSLVEKLQYKPESLAEEFSEFGSDFSRNNPKFDRLQQ
                     VIELTKALTKADIVAFYQKAVIEKQGFVFVSQAVGTKTKAEDVAKFDGFEQIESIEKL
                     QREFEIKDY"
     gene            complement(7449..9095)
                     /locus_tag="PXB27_000801"
     CDS             complement(7449..9095)
                     /locus_tag="PXB27_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005606119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5345619.1"
                     /translation="MKFPTLTKISASVLAVLALAACDDKSNENKPTAAAAKPQTEKTF
                     VNCVSRSPTGFSPILMMDGLSYNASSQQIYNRLVEFVPGTTDIEPALAESWEISEDGL
                     TYTFKLRKGVKFHSNKDFTPSRELNADDVVFSFNRQLDKNHPYHTVSNGTYPYFNAMK
                     FPTLLKSVEKVDDSTVRITLTKRDASFLSSLGMDFTSIYSAEYADKMMKAGIPEAVDT
                     TPIGTGPFVFSGYVLDQASRYVANKEYWKGKPDIDRLIFEIVPDATTRYAKLQSGQCD
                     LMDFPNATDIEKMKNDPKINLLSQPGLNIAYVAFNTEKAPFDNVKVRQALNLAVDKKA
                     IIDVVYQGNGFAAKNPLPPTIWGYNDSLADSEYNVEKAKQLLAEAGFPNGFETELWVQ
                     PVVRASNPNPRRMSEIIQADWEKIGVKAKLVTYEWGDYIKRTKAGELTAGTYGWSGDN
                     GDPDNFLSPLFGTPNIGNSNYARFTNPELDALLEKALGSSDKAERTKLYEQAQVILRE
                     QAPWINVAHSINFAPTSKRVEGYKQSPFGYTYLYPVKIVD"
     gene            9171..11261
                     /locus_tag="PXB27_000802"
     CDS             9171..11261
                     /locus_tag="PXB27_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HDL5345620.1"
                     /translation="MIFANQSIELDTVNVIATRDPSKFADTPQKQTKDALLVKQATSV
                     AAALKALPNVDVQGGARAIVQKPSIRGLSDNRIVQVIDGVRQNFDLAHRGSYFVPMSL
                     IQEIEVIKGPSSSLWGSGALGGVVAMRTPNALDLLKNNDKFGAKIRQGYQTANNLSET
                     EASVFAANDRFDALLSGFYNNADNLRSGEGKELNNTGYKQAGGLVKFGWQINDTHRLE
                     LSHRHSQFKQTAPGNNEVENQFTDNDVSAQISAWHAANPGNPSNPVYLAKMLAFYGQL
                     HSSLGSVSYLSDQKITDKSTSLNYYFNPSNPYLSTQVTLYSNSTTENEHRKISGVEDQ
                     TKLSTRGVNIRNHSELSWFSLVYGVDYMLDKVATQRGRNNTDAQYRANPYDAKSKTTG
                     AYLIAHIPLFDEKVVFSPSVRYDRFDTSSETVKYKDSHWSPAAKLTWKATNWLDLTAK
                     YNEAFRAPSMQERFVSGSHFGTTVRGLPINNIFLTNPNLRPETAKNKEVTFNVHFDNV
                     ISQGDKFNFEATYFRNDVKDLINLQVFKANPTSPMEFIPTRSQYQNIANARLSGIELQ
                     TRYQTERLAVFANYGSTKGKDKDSGEALSNIAASKIGFGVNYAVVQDKFTVGANVTRY
                     QAQHRVPKNHGVTYQGYTLTDLHATYAPLKGEWKNLRLDVAVENLFDKKYQPAFSLME
                     GSGRNVKLSAAYSF"
     gene            11332..13020
                     /locus_tag="PXB27_000803"
     CDS             11332..13020
                     /locus_tag="PXB27_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HDL5345621.1"
                     /translation="MKFYPSLIASAVSFAVVLSGQAAVNVPDAGSLQRELDQRQAQQK
                     VKPSGEWFNQAAKAPYTATDGQELTFPLTQVQVFDRTNQEISTPELAPILANYVGKAV
                     SLTDLSNLANEITEFYRSHNYLVAKAILPPQEIEQGKVKILLFKGNVGEVRLQNQSAL
                     SDKFVARLANTTVNGSEFILKDELEKFALTMNDVPGVNTGLELSAGKQAGEANLLIKL
                     QNAERFSGYVSADNQGNKNTGRYRLATGIKANNLIGWGDELKVDLMSSNNANLKNARL
                     DYSSLIDGYATRLGLTANYLDYELGGNFKALQSQGHTHNLGAYVLHPTIRLPDFRLNT
                     KVSFNHQRLTDKQQAVNVAQKRQVNSLTVGINGSWNSLKDGTSYFSLSALFGNLANHT
                     NEQAHYVATDFQPKSRFTVYQYSLSHEQALPKSFAFNLGVNGQFADKTLDSSQKMLLG
                     GLYGVRGHQAGVASVDEGHLVQTELKHYLPLFSKSLLTSSVFYDYGWGKYYKHSGFLA
                     RGVNNSVKLQSIGAGLSLSEAGSYAINASVAKPLGKKIENADKHQFWFSAIKTF"
     gene            13032..15848
                     /locus_tag="PXB27_000804"
     CDS             13032..15848
                     /locus_tag="PXB27_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HDL5345622.1"
                     /translation="MFKRNAISLCILTACSGLAYANTADLPQGERVRVGTATIERTTD
                     KMTVNQSSNKVQIDWNSFDIGKNKEVEFKQPTEMAVAYNRVTGGNASQIQGKLTANGK
                     VYLANPNGVLITKDAEVNVGGLLVTTKDLEKISENGTKDKFSRKLAKEGKILNQGKIT
                     AKGERAFVVLVGDDVANEGEINAKNYYHKETKKVTECYDDSWSLVKQCYDFDKEIVKS
                     TSGEVHLSSGENVTFTLSDRYIDVALDDNTVNSIIKNDGAIIANGDITLTAKGRNQAL
                     DSLIINNGVLQANKVQNKNGTIILSAGETQLHEKSQIKGEKVSFAEASYYGPTEDIKV
                     VSKTGSKVTAPVIDFKGKTVNIQGEFGREDSTDLYNDELQILKTTVNIEVPNTEDIRI
                     ADTDNGGSGSFIQAGALASLLANNGRVNVQGKGFDVSGKLNVNAFKQTDSVLKFINQA
                     GINIHNADIHANGRLFFVTNLPNDTDFQSNIRITDSKINLGNGSMGFGRSSKRWEMHD
                     VVRRADTELRKKFNLDMKNVELNQVDDFVVAGGFQQVNLDNVKARGKTNFYLDSGNSR
                     MFVGEQIYEYGVTDIEKRTQLANLDQRRKRWNSDNYDETDLNWEYNQRFDGFAAKHNH
                     RAPIADTEINIANSDISLDNGFVHLMAEKINLDDSKINITFNQDNSLDESTQINRLGL
                     NGKVSMKNSHINVVGDEKDGISPARHYATMFLVGELLGEKSSIFLKSHQGAVLRTDSN
                     VTIAGKNSKDDLKITAITTGKKMGNEIIGPDGLPTSEDNDANASNTAFAIGYEPNATA
                     IVENASITALAPNGGLAYTGTSKTQIDAKDNATFTFFEKPRAGYDKLLGDIKGNPDKL
                     TPRDFARLYDNIKGIKASSLSEQQLGLADTAKKTSEHTLANSLDVENLVSVTVCDEQN
                     KCEERMLGNKNTNAKVIVGDIKFE"
     gene            15971..16102
                     /locus_tag="PXB27_000805"
                     /pseudo
     CDS             15971..16102
                     /locus_tag="PXB27_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587011.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(16157..16918)
                     /locus_tag="PXB27_000806"
     CDS             complement(16157..16918)
                     /locus_tag="PXB27_000806"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDL5345623.1"
                     /translation="MKLNQAQIQNALICGQAVVFDEIDSTNEYLLTHHQRLENGTVCL
                     AEKQTAGRGRRGRTWYSPESENLYFSILWHYPMEQAADLPPLSLVVALIIAESLTVQN
                     VKDIQIKWPNDIYYQGKKMGGILLETQANRNGLDVVIGIGLNLGMAKVDENIVTQAWA
                     DLSQYHFNRNELVCRLAYELQKNLKIYPLVGFAHYAERWQSFDLFRHKAVKLITEAEE
                     IHGISQGINEQGELILKQGDNLRYFGIGEISLRAD"
     gene            complement(16930..17961)
                     /gene="murB"
                     /locus_tag="PXB27_000807"
     CDS             complement(16930..17961)
                     /gene="murB"
                     /locus_tag="PXB27_000807"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDL5345624.1"
                     /translation="MMKHSLTPFHTFHLPAKATQIIEFTTVEQLLSEWQKAFNAQLPI
                     LILGQGSNVLFLEDFDGVVLVNKLKGITHHEDEHFHYLHVQGGENWHELVKWTLAHNI
                     AGIENLALIPGVASSAPIQNIGAYGVEFEQVCDFVEVLNLRSGELFKLSKSECEFGYR
                     ESVFKHQYRDEFAIISVGLKLAKAWKPVLSYGSLAQLNPETVTPIQIFDEVCAVRSSK
                     LPNPDEFGNAGSFFKNPVISNEQFAPIQAKFPAIPHYPQADGTVKLAAGWLIDQTGLK
                     DLQIGGAAVHTQQALVLINKENATGQDVLALAKTVRQRVKEKFGVEIQPEVRFIGKNG
                     EVDSEKITR"
     gene            complement(18065..18739)
                     /gene="crp"
                     /locus_tag="PXB27_000808"
     CDS             complement(18065..18739)
                     /gene="crp"
                     /locus_tag="PXB27_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010787261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HDL5345625.1"
                     /translation="MQDMTLSTAPLENVTPPPSIHDPAVEWFLTHCHIHRYPAKYTLI
                     HAGEDAESLFYIVNGSVSVYIKDDETKEMLLTNLGTGEFVGELSLFEEKVQQRTAYVR
                     TKGPCEIAEISYKKFKQLVHLNPDILMYLSAQMARRLRQTSRQVGNLAFLDVAGRIAQ
                     TLLNLAKMPDAMTHPEGMQIKITRQEIGQMVGCSRETVGRIIKMLEDEGLISAHGKTI
                     VVYGAR"
     gene            complement(18801..19019)
                     /locus_tag="PXB27_000809"
     CDS             complement(18801..19019)
                     /locus_tag="PXB27_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HDL5345626.1"
                     /translation="MIIPWQELEESTLHNILDSFILREGTDYGERELSLAEKRERLLT
                     QLKADKVVIVWSELHESLDIKDKKSFLG"
     gene            complement(19022..19627)
                     /gene="slmA"
                     /locus_tag="PXB27_000810"
     CDS             complement(19022..19627)
                     /gene="slmA"
                     /locus_tag="PXB27_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HDL5345627.1"
                     /translation="MTEPKIKMPKKSVVERRQQVLEVLISLLNSEEGMQRITTERLAA
                     AVGVSEGALYRYFPSKTKMFEALIEKIEQTLTSYIHANKRQDSSANSVRAILYVILEF
                     ARKNPGVTRILTGHALMFEEELLKARVAKFFDNLELQFANILQLSKLRERKSFEDERA
                     LAGYLVNFCEGQFLRLVRSNFSYNQHQHFEKQWAFIKPLFD"
     gene            complement(19639..20094)
                     /gene="dut"
                     /locus_tag="PXB27_000811"
     CDS             complement(19639..20094)
                     /gene="dut"
                     /locus_tag="PXB27_000811"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014550997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDL5345628.1"
                     /translation="MKQIDLKILDSRIGNEFPLPAYATEGSAGLDLRAMLNEPLTVKA
                     GETVLIPTGISIYIADPNLAAVILPRSGLGHKNGIVLGNLVGLIDSDYQGELKVSLWN
                     RSQADFTVNVGDRIAQLVFVPVVQASFNIVQEFEQTDRGEGGFGHSGKQ"
     gene            complement(20128..>20364)
                     /locus_tag="PXB27_000812"
     CDS             complement(20128..>20364)
                     /locus_tag="PXB27_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDL5345629.1"
                     /translation="YNYQTEHLLDDIAQQKISPNYIPHIIHFTGGIKPLEGKFNSSYV
                     ENVASLFRFYHNTSWEILTELPLGTIQLKPFITE"
BASE COUNT         6114 a         4213 c         4420 g         5617 t
ORIGIN      
        1 cgaggtaaaa gtgaataaat ataaaaatgc cagtgcatta ttggtttcat taatacttat
       61 ttctacaatt ttgtcggttt tatttttaag taaagaaaaa tggcttggac aacaaaacat
      121 tacccatttt tatagagaga aatacctttc cgatcaatat aattttttag cgttatatcc
      181 acaagataaa acgcaaatat gccaaaaatt tcaaaaagag cagatcagcc aagcggattt
      241 atccgataaa caattcaggt ttttacagta tcaatttgcc tgccaattcc atagtttatt
      301 taaagataaa aaaccgacta aagagaaata tattcacttt acccatttgg cggattattt
      361 agatttatca aatgtgccaa accaagagat ttatactatt cattctttag ctgaattacc
      421 tagtagcagt gaacgtgagc ctaaaattgt gcttgctaaa aacgcaatta atgaaaactt
      481 accgcaagag ttttacggca ttgtgataac cgactatctg tttgatatta ccggcaaaaa
      541 aatgtacggc acgctttatt cctcttatga taatgcccga gaggagcgaa atttaacgtt
      601 taaaaaagag gtactggcag agcttgaggc gaggtattcc gcttggcgat atttgccata
      661 ttccgaacat ttgatgggag cggttgatga ttaaatataa agcagaaacg gctttgtcgc
      721 ttttggttgc catcacgcta tttgcgattg tggtgttgag tttcagccag tggcaatcgg
      781 cacaaaacca caggctaaat caacatttcc aacagcaaca agcggttatg attttagaaa
      841 atcagcttgc cctcaaatta gccgggttag attgtgaatc aagtattact caaaatcaga
      901 ttcgctatca gattcaatgc tctggcaatc gcttaaccat tcgctttccg ttaggcaaaa
      961 ttgagctgaa taatgattga atcgtccctc gctttggagg acaataattc aattagcgag
     1021 gcgttattat gtttacactt taccaatcca accaaatttc atcactggcg gagatgctgg
     1081 tgaaaatcca gcaggtaaac ccacttgaag atccgtttga gccggaaacc attttgatcc
     1141 aaagtcaggg aatggcacag tggctacaaa tgcagattgc tgaattaaac ggtgtgatgg
     1201 ggaattgcga ttttctctac cctactacat ttttatggca acagtatcgg ctgttgttcc
     1261 cggagctgcc gaaagagaat atttttgagc gtagttcgtt ggtttggcgg attatgcggt
     1321 tattgccgag ttgcttgaca ctatcggagt ttgctccgct aaagcgttat ttaggtgatc
     1381 aacctaataa agaacagtat cagcttaagc tctatcaact ttctgccaaa attgccgaat
     1441 tgttcgacca atatttggtg tatcgccccc attggctggt tagctgggaa gagggcaact
     1501 tacaagcgat cgaaaatgag ataaaaattg caactgccgc taagccgatt gattttgact
     1561 taattcgagc cgatatgaaa tggcaaagcc atttatggca acagattgta tctgatctgc
     1621 aatcggaatc tgatgagcag gtttttacta cctcccaccg tgcctatttg caacaaaatt
     1681 acttcgctaa gttagatgac cttactgaaa cggaaaaagc caaattgcca aaacgcattt
     1741 tcatttttgg gatttcgtcc ttctcgccat tacagttagc ggtgtttaag aaactcagcg
     1801 agcattgcga tattcacctg ttctttttta acccaagcga aaaatattgg ggcgacagtg
     1861 tggaagaaaa agtgtggcaa aaattggcgt taagccacaa gatttcgcaa gacgatctgg
     1921 ataacttatt ggcggagcaa agaaataaac tactgaatct ttggggcaaa caagggcggg
     1981 atttcttagc tcaattaatc gaatttgagc cgaataccat ggatgtcttt gtcgaaccag
     2041 aggaaaacag caacctcaat aaactcaaaa aacagatttt cagcttagaa aatgacgcca
     2101 ctattaccga tttaaccgcc gataattcac tccaaattca ttcgtgccac agcccaatgc
     2161 gggaagtgga agtgttgcat aaccagctcc tgaatttgtt tgagcaagat agcactttgc
     2221 tgccgaaaga tattattgtg atgtcgccgg atattaataa atacgctcct tacattgatg
     2281 cggtgttttc tcgctacaat acgggtaatc gggatcctcg ctatattcct ttttcattat
     2341 ccgaccaagc atttacgcag gttgatccga ttatccacag tttcctgcaa ctgctggcaa
     2401 tgaaagaaag cccgtttaat gccgaagaac tgtttgattt actggaagtg aatgcgatcc
     2461 aaacccgttt tggctttagt acggaagata ttcataccct gcgggtttgg gtgagaaatg
     2521 ccggcgtacg ttcgggtgta gaaatcgagc aacctcactg gcaaaactac aattcgtggg
     2581 aaaacgggct aaaccgttta ttgcttggct caagcctgaa agaagagcag gggatttggg
     2641 aaaacaccct tgcctttggg gatagctatg gcttaaatgc cgagctggtg gggcgacttg
     2701 cctcttttat tgaggcttta ttagcgtggg taaactttct gcaaacgcca cagccgttgg
     2761 cagcgtggca gcaggcaata aaaaatttga ctgctgatct gtatcaagaa aatgccgaaa
     2821 atgctgcatc aattttattg ttgaataatg tgaatgatca gattatcgag caaatttccg
     2881 caacccattt ttcagacgat ctgcaaattg aggttttagc cttattgttt gagcagcagc
     2941 tcagccaaca ttcacagcag cttaatttct tggtcggacg ggtgaatttt gccaccttgc
     3001 ttccgatgcg ggcgattccg tttaaggtaa tttgcttgct gggtatgaat gaggcagatt
     3061 tccctcgcca acaacaaatc aatagctttg atttaatgca gtacgccccg aaaaagggag
     3121 accgtgccaa acgtgatgat gaccgctact tgttcttaga agccttactt tccgctcagc
     3181 aaatgtttta catcagctat atcgggcagt cgcaaaaaga cggcaaagag atgctaccgt
     3241 cgattttggt ttcccaactg ttagatgcaa tgaatgaagc tttaacgcct gaatgcaagc
     3301 aacaattaag cgatgaagtg gaagttttgg tcagaaaaca acctttaagt gtgtttagca
     3361 aaaagaattt tacccaccca cgagatcacg cttataacgc agagtggatt atcaagccga
     3421 ctgttgccgt acaagatttt cttagtgagc ctttaaccaa tgaaagcgtg gcttcgctag
     3481 aacttgatga attaatctct tttgtacaaa acccgattag gtttttcttt ctaaaagtgt
     3541 tggggatttc gtttaacact tatgatgaca ccattgatga aagcgaaatt tttgagatgt
     3601 cggggctgga taactaccgg attttaaacg aattattaac gataaattta gcacaacagc
     3661 aacgtttttt tgccgaagag caactcaaag gcaatttacc tgcttcttat tttgctcaaa
     3721 ttgccacaca agatttgatc ggcaaaatta ccgaaatgca agcggcgtta agccactatt
     3781 tagccagcga aagccgtatt ttgagtgtta atcgggaggt tgagctggaa cgccaaaccc
     3841 tcaccctaaa cggcaatatt gccaacctat atcagaatga agtggtgcta tggcgagtag
     3901 ggcatttacg agataaagac aagattcaga cttggatcta ccatttatta ttgtgtgcct
     3961 atttccctga aaaaacggtg aaattctatt accgagaggg tgagcaagtt ggtgtgttga
     4021 gctttgaaac cgtctcgcag atagaggcat tatcgcaatt aagcatttat ttgaatgctt
     4081 atttggacgg attatcgcaa gcccagttag tgatctacga ccgaattgag gaatatctca
     4141 aaggtgatgg cacaaacagc gaacaagttc ttatcgattt agcagagaaa gatgagcgag
     4201 acggcagcta tctccatcgg cttctcgctc aaactaaaga gctggattct caagcgattc
     4261 accaacgtac tcaaagctgg tttagcttaa tgaatcagaa aatagcgaaa ggcgattaac
     4321 tacaagcggt cgtttttggc tgaaaatttg caaaatgaga aggcgaataa taattcgcct
     4381 acctataaaa atagaatttt ttccgtattt tactgtcaat ttattactac aatgctaatt
     4441 tcaaaaggag atgtatgaaa ttatcttttc ttacccaatt cgttgcagcg gcttttgccc
     4501 tcaatgtgat gacttaccct gcatttgcgg aagttgcaaa aaattctgaa aaaacgaccg
     4561 ctagtcaacc ccaatcccaa ctcggttttg agctgattaa acagagcatt aacaaaagcc
     4621 cgaatgataa agcgatttat caaggtatta aacttgataa tggaatggaa gtgctactga
     4681 tttccgacag tcaagcgaat aaatcgttga tgtctgccat tattccgatt ggctcaatgg
     4741 atgacccgat ttctcagcaa ggtttagccc actatcttga gcatatgatc ttgatgggct
     4801 cgaaaaacta tccggaaacc aacagcttag acggtttttt aaccaaaaat ggcggtatga
     4861 ataatgcctc gactgcttct caccgcacgg cgtattatct acaagtcaat aacgaagcct
     4921 ttagcgaagc ggtaacacga ctggcggata cgttagcttt cccgttattg ctggaaacca
     4981 acgccaagaa agaggttaat gcggtcaatg cggagatggt gcgagctaaa tcgcacgatg
     5041 ggcatttatt gcacagtgtt aatttagcca ccgcaaaccc ggctcatcct gctaccaaat
     5101 ttgcggtagg caataaagag acgttatctg acaagccgga tagcaaatta caaaccgagt
     5161 tggagcaatt ttataaacag cattattctg cgaatctgtt taaagtggtg ctttattcca
     5221 atcaatcgat tgagcagatg gcaaaattgg cagccaacac tttaggcaaa atggaaaaca
     5281 aacatttcag cataccgcaa accgctgagc cacgttatcg tgatgaggac aaatcggtat
     5341 ttattcaata taagccggtg aaatccaaca aaatgttagt cattggcttt gattttccga
     5401 atgacgaaat gcagtttaag cacaaaaccg gtaaatatat tgcttatatg ttaaacaata
     5461 atactgacgg cacgctttcg gattacttga ttaaacaggg gttgtctgac ggcggtattg
     5521 aggcggttag cagtgccaat atcgggcggg atcgaggctc gtttgacatc tatattgaac
     5581 tgacagacaa aggtttgagc gagcaagatc aaattatttc attggttttc caacaaattg
     5641 agaagatcaa acaagaaggc attcaagaga gctattttag cgaagtgaaa gagagcctga
     5701 agcaggattt ccaacatttg caagtggaaa aaaatatgga tttcgtggaa tttattgccg
     5761 atcaaatgct ccatttcccg ttagaacacg ttatcgatca gccttatctg accgaaacaa
     5821 tggataacga ggcaattaaa gcaaaattgg cggcaatgaa tattgataat gcccgcatta
     5881 tgttagtcag cgatactgcc aaaaccgaca agaaaacccc ttattttgaa gcaggttatt
     5941 cggtggcaaa aatcacgcaa gaacaaaagc aaaaatggct ggatttcagt aaaaatccac
     6001 agctcaattt gcctgaattg aacccgtatt tcactaccga ttttacctta aacaaggtcg
     6061 aaaccggctt aactaaaccg aaattgatta acgaccaagc ggggaattta atctatacaa
     6121 tgccgagcgt gtattttacc aatgaaccga aggctaaaat cggcgttggc tttaatattc
     6181 gcccgagaaa agatgactta aagagcctga ccgcagcgtc tattttgggt tatatgaaca
     6241 atttagccca aaccaagtta gattttcagt cttcggtggc gggtatgaat ttaagtttag
     6301 ctccggcggc aaacggttta gccttaagtg cagagggtta tacccaaaat ctggctaaat
     6361 taatgctaga cagcgtaaaa cagttcagcc gctttgagtt taacgaagac gtgttggcac
     6421 aggctaaaca acgtttgcac caagagctgg atcgtaacga aaaagaaaac agccttaatc
     6481 aagctaatgc agcattaagc cgttttgcct cttaccctta ttttgatgtg ccgaaacagc
     6541 gtgagatgat tgatagcatt acccttgcgg atattgagca actacgccag cgaattttgc
     6601 agcagagcag cggctttaag ttgttatcgg tggggaattt gtcggctgag caggtttctg
     6661 ctctggcaga ggatttacaa gcggtcataa aaaaccaaaa atctgcaaat gctaccggac
     6721 agtatgtgga tgtgagtacc agtcagcgta agattaatgt gattaaaagc gtgccgaatg
     6781 aagataatgc gttgctaatg gcatttatgc cgaacggcta taacgagctg gaaagcctta
     6841 ctcgagccaa attgctcaga agcattattt cccgttggta ttttgatgat ttgcgtactg
     6901 ataagcaact aggctatgtg gtttatgcga cagataatat catcgggaaa accgccggta
     6961 tccagtttat ggtacaaagc ccgaatacca caccggcagg gattttagag cataacgaac
     7021 gtttctttac acaaagtttc gaacgtttaa aaaatctatc tgatgaggaa tttggaaaat
     7081 atcgtgatag cctagtggaa aaactgcaat ataaaccgga gtctttagcg gaagagtttt
     7141 ctgagttcgg ttcagacttt agcagaaata acccgaaatt tgaccgcttg caacaggtga
     7201 tcgaattaac caaagcactc accaaagcag atattgtggc attttaccaa aaagctgtga
     7261 ttgaaaagca aggcttcgtg tttgtcagcc aagctgtcgg caccaaaacc aaagctgaag
     7321 acgttgcgaa atttgacggt tttgaacaga tcgaaagcat tgaaaagcta caacgagagt
     7381 ttgagattaa ggattattag agagttatta gagagagtta ggggcgaaaa attttcgccc
     7441 ctcatttttt aatccacaat tttcaccgga tataagtaag tatatccgaa cggactttgt
     7501 ttgtagcctt caacacgttt gctggttggg gcaaagttaa ttgagtgagc cacgttaatc
     7561 cacggagctt gctctcttaa gataacctgt gcctgctcgt ataacttagt acgttctgct
     7621 ttatcagatg agcctaatgc tttttctaat aacgcatcta actcagggtt agtaaagcga
     7681 gcatagttac tgttgccgat atttggcgtg ccgaatagtg gcgataagaa gttatccgga
     7741 tcgccgttat cgcccgacca accgtaagtt ccggcggtta attcaccggc tttggtacgc
     7801 ttgatgtaat caccccattc ataggtgact aattttgctt ttacgccgat tttttcccag
     7861 tcggcttgaa taatttcaga catacggcgt gggttcgggt tagaagcacg caccacaggc
     7921 tgcacccata attcggtttc aaaaccgttc gggaagcctg cttcagctaa tagttgcttc
     7981 gctttttcca cgttatattc tgaatccgct aagctgtcgt tatagcccca aatggttggc
     8041 ggaagtgggt ttttcgccgc aaaaccgtta ccttggtaaa ctacatcaat aatcgctttt
     8101 ttatccactg ctaagtttaa cgcttggcga actttcacgt tatcaaacgg agctttttcg
     8161 gtattgaatg ccacataagc aatgttcaaa cccggttgag aaagtaaatt gattttcgga
     8221 tcatttttca ttttctcaat gtcggttgca tttgggaagt ccattaaatc acattgaccg
     8281 gattgtaatt tcgcataacg ggttgttgca tccggcacga tctcaaaaat caaacggtca
     8341 atgtcaggct tacctttcca atactctttg tttgctacat aacgactggc ttggtctagc
     8401 acataaccgc taaacacaaa cggacctgtg ccgattggcg tggtatctac cgcttcaggt
     8461 atgccggctt tcatcatttt atccgcatat tccgccgaat aaatggaggt gaaatccata
     8521 ccaaggctgg ataagaatga cgcatcacgt ttggttaagg taatacgaac ggtgctgtcg
     8581 tccacttttt ccaccgattt caataatgtc gggaatttca ttgcgttgaa atacggataa
     8641 gtgccgttag agacggtgtg atacggatga tttttatcta actgacggtt aaatgaaaat
     8701 actacatcgt ccgcattcaa ttcacggctt ggagtgaagt ctttgtttga atggaatttc
     8761 acgcctttgc gtaatttaaa ggtgtaagtt aagccgtctt cgctgatttc ccagctttct
     8821 gccaatgccg gttcaatatc ggttgtaccc ggcacaaatt ccaccaagcg gttgtaaatt
     8881 tgttgcgagc tggcgttata agataaaccg tccatcatta aaatcgggct aaaaccggtt
     8941 ggcgaacggc taacgcagtt tacaaaggtt ttctcggttt gcggttttgc tgctgccgca
     9001 gtaggtttat tttcgttgct tttatcatcg caagccgcta gagctaatac cgctaaaacg
     9061 cttgcactaa ttttggttaa cgttggaaat ttcatattat tctccgctta atcttaggat
     9121 taacttacga agattacctt ttccacaatg aggagtaaag aacaaaaagc attatttttg
     9181 caaatcaatc catagaatta gataccgtca acgtcattgc cacccgtgat ccaagtaaat
     9241 ttgccgatac gccacaaaaa caaactaaag atgcactttt ggtaaaacaa gcgacgagtg
     9301 tggcggcggc attaaaagcg ttgccgaatg tggatgtgca aggaggggcc cgtgcgattg
     9361 tgcagaagcc gtctattcgt gggttgagcg ataatcgtat tgtgcaggtg attgacggag
     9421 tgcgtcagaa ttttgatttg gctcaccgtg gttcttattt tgtgccgatg tcattgattc
     9481 aagaaattga agtaatcaaa ggtccaagca gctcgttgtg ggggagcggt gccttaggtg
     9541 gggtggtggc aatgcgcaca ccgaatgcgt tggatttatt gaaaaataat gacaaattcg
     9601 gggcaaaaat ccgtcaaggt tatcaaacgg cgaataatct ttccgaaacc gaagcctcgg
     9661 tgtttgcagc aaatgatcgg tttgatgccc tgctgagtgg tttctacaat aatgctgaca
     9721 atttgcgtag cggtgaaggc aaggaactga ataacacggg ctataagcaa gccggcggtt
     9781 tagtaaaatt cggctggcaa attaacgata ctcatcgttt ggaattatcc catcgtcata
     9841 gccaatttaa acaaactgca ccgggtaata acgaggtaga aaatcaattt accgataatg
     9901 atgttagtgc acaaattagt gcttggcatg cggctaatcc tggaaatcca agtaatccag
     9961 tatatcttgc gaaaatgtta gccttttacg gtcaattaca ttcaagtctt ggtagtgtga
    10021 gttatttaag tgatcaaaaa attaccgata aaagcacctc acttaactac tatttcaacc
    10081 caagcaatcc ttatttgagc acacaagtga cattatatag caatagtacc actgaaaacg
    10141 aacatcgaaa aataagtggt gtggaagatc aaactaaact aagcactcgt ggtgtgaata
    10201 tccgtaatca ttccgaattg tcttggttct ccttggtgta tggcgtggat tatatgttgg
    10261 ataaagtagc cactcagcgt gggagaaata atacggacgc acaataccgt gctaatcctt
    10321 atgatgctaa atctaaaact acaggtgctt atttaattgc acatattccg ctgtttgatg
    10381 aaaaagtggt cttttctccg agcgtgcgtt acgaccgttt cgatacttca agtgaaacgg
    10441 tgaaatataa agacagccat tggtctcctg ctgcaaaact tacttggaaa gcgacaaatt
    10501 ggttggattt aaccgcgaaa tataacgaag ctttcagagc cccttctatg caagaacgat
    10561 ttgtgagcgg gtctcatttt ggtaccacag tgagaggatt acctattaat aatattttct
    10621 taactaaccc taatttacgc ccggaaacgg caaaaaataa ggaagtaacc ttcaatgtac
    10681 attttgataa tgtcatctcg caaggagata aatttaactt tgaagcgact tatttccgta
    10741 atgatgtgaa ggatttgatt aatcttcaag tattcaaagc aaatcctaca agcccaatgg
    10801 aatttattcc cactcgttcc caatatcaaa atattgcgaa tgcacgtttg agtggtattg
    10861 aattacaaac tcgttatcaa actgaacgtt tggcagtttt tgctaactac ggtagtacaa
    10921 aaggtaaaga taaagacagc ggggaagcct tgtcgaacat tgctgcaagc aaaatcggct
    10981 tcggggtcaa ttatgccgtg gtgcaagaca aattcacggt gggggcaaac gtaacccgtt
    11041 atcaagcaca acatcgtgtg ccgaaaaacc acggcgtgac ttaccaaggc tatacgctga
    11101 ccgatctaca cgccacttac gccccattaa aaggcgaatg gaaaaacctg cgtttagacg
    11161 tggcggttga aaacctattc gataaaaaat accaaccggc attcagctta atggaaggca
    11221 gcggtcgtaa tgtgaaactc agtgcggctt acagcttcta atgtctttaa cggaagacgg
    11281 cacaagcggt cgttttccgg ttattttttg caatattaaa agggagttac tatgaaattt
    11341 tatccgtcgc tgatcgcaag tgcggtcagt tttgccgttg ttttgagtgg acaagcggca
    11401 gtgaatgtgc cggatgccgg ctcgttacag cgtgaattag accaacgtca agctcaacaa
    11461 aaagtcaaac cgagtggcga atggtttaat caagcggcaa aagcacctta taccgcaacg
    11521 gacggtcaag aactgacatt tccattaact caggtacaag tttttgatcg aaccaaccaa
    11581 gaaatctcta ccccggaatt agccccgatt ttggcaaact atgtgggtaa agcggtttcg
    11641 ctgaccgatt taagcaactt ggcaaatgag atcaccgagt tttatcgtag ccacaattat
    11701 ctggtggcaa aagccatttt accgccacag gaaattgaac aaggtaaggt gaaaatcctg
    11761 ttgtttaaag gcaatgtcgg tgaagtgcgg ttacaaaatc agagtgcttt aagcgataaa
    11821 tttgttgcac gccttgccaa caccacagtg aatggttcgg aatttatcct aaaagacgaa
    11881 ttggaaaaat ttgccctcac gatgaatgat gttcccgggg tgaataccgg cttggagctt
    11941 tctgccggta agcaagcagg cgaggcgaat ttgctgatta agctccaaaa tgccgagcgt
    12001 ttctcaggct atgtatcggc agataaccaa ggcaataaaa ataccggacg ttaccgttta
    12061 gccacaggga taaaagccaa taatctgata ggttggggcg atgagttgaa agtggatttg
    12121 atgagttcca acaacgctaa tttaaaaaat gcgcgtttgg attattcgag cttgattgac
    12181 ggctacgcta cccgtttggg cttaacggcg aattatctcg actacgaatt aggcggtaac
    12241 tttaaagccc tgcaatccca agggcatact cacaatcttg gggcttatgt gttgcacccg
    12301 acaatccgct tgccggattt tcggttaaac accaaagtca gctttaatca tcaacgttta
    12361 accgacaaac aacaagcggt caatgtggca caaaaacgcc aagtaaacag cttaacggtg
    12421 ggcattaacg ggtcgtggaa ttcgcttaaa gacggcacaa gctatttctc tctgtctgcc
    12481 ttgttcggca atttagctaa ccacaccaac gagcaagcgc attatgtggc aacagatttt
    12541 cagcccaaat cccgttttac ggtctatcaa tacagcctgt ctcacgaaca agcgctgcca
    12601 aaatcctttg ccttcaacct tggcgtgaac gggcagtttg cggataaaac cctcgacagc
    12661 tcacaaaaaa tgttactggg cggtttatac ggcgtgcgtg gtcatcaagc cggtgtcgct
    12721 tcggtggatg aggggcattt agtgcaaacc gaactcaagc attatttacc gctcttttcc
    12781 aaaagcctgc tgaccagctc ggtcttctat gattatggtt ggggaaaata ctataaacac
    12841 agcggatttt tggcgcgtgg tgtaaacaat agcgtgaaat tacagagtat cggagcaggg
    12901 ctttcgctct ctgaagcagg cagttatgcc attaacgcca gtgttgccaa accgctgggt
    12961 aaaaaaatcg agaatgcaga taaacaccaa ttctggttct ccgccatcaa aaccttctaa
    13021 ggagacaaac tatgtttaaa cgtaatgcaa tttctctctg cattttgacc gcttgcagcg
    13081 gtttagccta tgccaataca gcagatttgc cgcaaggtga aagggtgcgt gtcgggacgg
    13141 caacgattga gcgcacaacc gataaaatga ccgtgaatca atccagcaat aaagtacaaa
    13201 ttgattggaa cagctttgat attggtaaga ataaagaagt ggaattcaaa cagccgacag
    13261 agatggcggt ggcatataac cgtgttaccg gcggaaacgc cagtcaaatt caaggcaagc
    13321 tgaccgcaaa cggcaaagtg tatttagcca acccgaacgg tgtactcatc accaaagatg
    13381 cggaagtgaa tgtgggcggt ttgttagtga ccacaaaaga tttagaaaaa atcagcgaaa
    13441 acggcacaaa agataaattc agtcgcaaat tagcgaaaga aggcaaaatt cttaaccaag
    13501 gtaaaattac ggccaaaggc gagcgggctt ttgtggtatt ggtgggcgac gatgtggcta
    13561 atgaagggga aattaacgcc aaaaattatt accacaaaga aacgaaaaaa gtcaccgaat
    13621 gttatgacga ttcttggagc ttggtaaaac aatgctacga ttttgataaa gaaatcgtga
    13681 aaagtaccag tggagaagtg catttatcct ccggagaaaa tgtcaccttt actttaagcg
    13741 accgctatat tgatgtggcg ttagacgata atacggtcaa tagcattatc aaaaatgacg
    13801 gggcaatcat cgccaatggt gacattaccc tcactgccaa agggcgaaat caagccttag
    13861 acagcttaat tatcaataac ggcgtattgc aagccaataa ggtgcaaaat aaaaacggta
    13921 cgatcatttt atctgccggt gaaacccaat tacacgaaaa aagccaaatt aaaggcgaga
    13981 aagtgagctt tgccgaagct tcttattacg gtccgacaga agacattaaa gtggtttcca
    14041 aaaccggctc aaaagtgacc gcacctgtga tcgatttcaa aggtaaaacc gtgaatattc
    14101 aaggggaatt tgggcgtgaa gacagcaccg atctctataa cgatgaacta caaatcctca
    14161 aaaccacggt gaatattgaa gtcccgaata cggaagatat tcgcattgca gataccgaca
    14221 acggaggtag cggttccttt atacaagcgg gtgctttagc ctcattactt gcaaataacg
    14281 gacgggttaa tgtacagggc aaaggttttg atgtctcagg caagttgaat gtgaatgcct
    14341 ttaaacaaac ggattccgtg ctgaaattta tcaatcaggc gggcatcaat atccacaatg
    14401 ccgatattca cgcaaacggg cgtttattct ttgtgacaaa cttgccgaat gacaccgatt
    14461 tccaatcgaa tatccgcatt accgacagca aaatcaactt aggcaacggc tcaatgggct
    14521 ttggtcgcag tagcaaacgt tgggaaatgc acgatgtggt tcgacgtgcc gatacggaac
    14581 tgcgtaaaaa attcaatctg gatatgaaaa acgtggaatt aaaccaagtg gatgattttg
    14641 tggtagcggg cggtttccaa caagttaatt tggataacgt gaaagcccgt ggcaaaacca
    14701 acttctatct ggattccggc aactcccgca tgtttgtcgg tgagcagatt tatgaatatg
    14761 gcgtaaccga tattgaaaaa cgcacccaat tggcgaattt agaccagcgt agaaaacgtt
    14821 ggaattcgga taactatgat gagaccgatc tcaactggga atacaaccaa cgctttgacg
    14881 gctttgcagc aaaacacaat caccgcgcgc cgattgccga tacggaaatc aatattgcca
    14941 atagtgatat cagcttagat aacggttttg tgcatttaat ggcagaaaaa atcaatctgg
    15001 acgacagcaa aatcaatatc accttcaacc aagacaacag cttggatgaa agtacccaaa
    15061 tcaaccgctt aggactaaac ggcaaagtgt cgatgaaaaa cagccatatt aacgtagtgg
    15121 gcgatgaaaa agacggtatc tcaccggctc gccattatgc cacgatgttc ttagtggggg
    15181 agctgcttgg tgaaaagagt tctattttcc tcaaatccca ccaaggtgca gtattgagaa
    15241 ccgacagcaa tgttacaatt gccggtaaaa acagcaaaga cgacctcaaa attaccgcca
    15301 ttaccaccgg taaaaaaatg ggcaatgaaa tcatcggacc ggacggcttg ccaacctcgg
    15361 aagataacga tgccaacgcc agcaacaccg cctttgccat cggctatgaa cctaacgcta
    15421 cggctatcgt cgaaaatgca agcattaccg cattagcacc aaacggcggc ttggcataca
    15481 ccggcacgtc taaaacccaa attgacgcga aggataatgc tacctttacc ttctttgaaa
    15541 aacctcgagc cggctatgac aaattgcttg gcgacatcaa aggtaacccc gataaattaa
    15601 ccccacgcga tttcgcccgt ttatacgaca acattaaagg catcaaagcc agcagcctat
    15661 ccgaacaaca gcttggccta gcagacaccg cgaagaaaac cagcgaacac accttagcaa
    15721 acagcctaga cgtagaaaat ttggtttcag tgacagtgtg tgatgagcag aataagtgtg
    15781 aggagcgtat gttaggtaac aaaaatacca atgctaaagt tattgtcgga gatatcaaat
    15841 ttgagtagcg ttgaagaaag tggtagaagc tcagacttga tctgataaag cactataaaa
    15901 agtgagagtt tcccgtttag aatatgagtg tcaaaattca atctaaacaa aaaggaaact
    15961 ctcatgtttt attacttaca atcccctcat taaacacaag accggtttat taaatttagc
    16021 agaagaaccg ggcaatatct ctcaagcctg caatgtggtg ggtatgagcc tagatacatt
    16081 ctattgctat caacaagcgg ttatttttgg caactaattt gcaaaaaaac aataaaatcc
    16141 gaccgcttgt agagggttaa tccgccctta gtgaaatttc accgatgccg aaatagcgga
    16201 ggttgtcgcc ctgttttaga atgagttcgc cctgctcgtt aatgccttgc gaaatgccgt
    16261 ggatttcttc ggcttcggta atgagcttaa ccgctttatg gcggaaaagg tcgaaacttt
    16321 gccagcgttc ggcataatga gcgaagccga caagcggata aattttgaga tttttttgca
    16381 attcataggc taaacggcaa accaattcgt tgcggttgaa atgatattgc gataaatctg
    16441 cccacgcttg cgtcacaata ttttcatcca cttttgccat tcctaggttc agcccaatgc
    16501 cgataaccac atctaaacca ttgcgattgg cttgggtttc caacaaaatg ccccccattt
    16561 ttttgccttg gtaataaatg tcattcggcc atttaatttg aatatctttt acattttgaa
    16621 cagtaaggct ttccgcaatg atcaaggcta ccactaaact taagggcggt aaatctgccg
    16681 cctgctccat cgggtaatgc cataaaatcg agaaatagag attttcactc tcgggcgaat
    16741 accaagttcg acctcgtctg ccacgtccgg cagtttgttt ttcagcgagg caaaccgtgc
    16801 cgttttccaa acgttggtga tgagtgagca aatattcatt ggttgaatca atttcatcga
    16861 acacaacggc ttgcccgcaa attaaagcat tttggatttg agcttggtta agtttcatta
    16921 tttccccact tagcgagtga ttttttcgct atcgacctca ccgtttttac cgataaaacg
    16981 cacttcaggc tgaatttcta ccccaaattt ttctttcact ctctggcgaa cggttttggc
    17041 tagagccaag acatcttgcc ctgttgcatt ttctttatta atcagcacta aggcttgctg
    17101 ggtatgtacc gccgctccgc caatttgtaa gtctttcagc ccggtttggt caattaacca
    17161 acctgcggct aattttaccg ttccatcagc ttgagggtag tgaggaatgg ctgggaattt
    17221 ggcttgtatt ggtgcaaatt gttcgtttga aatcaccgga tttttaaaaa agctgccggc
    17281 attgccaaat tcgtcagggt ttggcaattt ggaggagcga accgcacaga cttcatcaaa
    17341 aatctgtatt ggcgtaacgg tttccggatt gagttgggct aatgatccgt aacttagcac
    17401 cggtttccac gctttggcta actttaagcc taccgagata atcgcaaatt catcacggta
    17461 ttggtgttta aacacacttt cacgatagcc aaattcgcac tcggatttgg atagcttaaa
    17521 gagttctccg gaacgtaaat ttaacacttc cacgaaatca catacctgtt caaattcaac
    17581 cccataagct cctatatttt gaatgggagc agagctggcg acacccggaa ttaacgccag
    17641 attttcaatg ccggcaatat tgtgtgccaa cgtccattta accaattcgt gccagttttc
    17701 cccaccttga acgtgcaaat agtggaaatg ttcgtcttcg tggtgcgtaa tccctttgag
    17761 tttatttact aacaccacac cgtcaaaatc ctctaaaaac aaaacgttag agccttgccc
    17821 gagaatcaag attggcagtt gagcgttaaa ggctttttgc cattcgctca atagctgttc
    17881 gactgtggta aattcaataa tttgggtcgc ttttgcaggc aaatggaaag tgtgaaaagg
    17941 ggttaagcta tgtttcatca tcaaattagt tagttaagaa gtggaaataa caagcggtga
    18001 tatttttaca aaattttgca attaagattt tgggcaggca caggatctgc ccctacattc
    18061 agaattacct tgccccatat accacaatgg ttttgccgtg agcagaaatt aagccttcgt
    18121 cttccaacat ttttataatg cgtccaacgg tttcacggga acaaccgacc atttgcccga
    18181 tttcttggcg ggtaattttg atctgcatac cttccgggtg agtcattgca tccggcattt
    18241 tagctaagtt gagcaaggtt tgagcaatgc gaccggcaac gtctaaaaag gcaagattac
    18301 cgacttggcg tgaagtttga cgtaaacggc gagccatttg tgccgataaa tacattaaaa
    18361 tatcggggtt taaatggact agttgtttga attttttata ggaaatttcc gcaatttcac
    18421 aaggaccttt agtgcggacg taagcggtac gttgttggac tttttcttca aataaactta
    18481 attcaccgac aaactcacct gtgcctaagt tagttaagag catctctttg gtttcatcat
    18541 ctttgatgta aaccgacact gagccattca cgatgtagaa aagtgattct gcatcttcac
    18601 cggcgtgaat gagtgtatat ttcgccggat aacgatggat atgacaatgg gttaaaaacc
    18661 actccaccgc agggtcgtga atagagggcg gaggagttac attttccaga ggagctgttg
    18721 aaagtgtcat atcttgcata gtatttccta taactcttat actaggttaa atttttagaa
    18781 ttttaacacg aaattaaagg ttagcctaaa aaagattttt tgtctttaat atcaaggctt
    18841 tcgtgtaatt ccgaccaaac aatcaccact ttatctgcct taagttgtgt gagtaatcgt
    18901 tctctttttt ctgccagaga aagttccctt tccccgtagt ctgtgccttc tcgtaaaatg
    18961 aaggaatcta aaatattatg taaggttgat tcttcgagtt cttgccaagg tataatcata
    19021 attaatcaaa taaaggctta ataaatgccc actgtttttc aaaatgttgg tgttggttat
    19081 agctgaaatt agaacgaacc aagcgtaaaa attgcccttc gcagaaattc actaaataac
    19141 cggctaaggc tctttcgtct tcaaagctct tgcgttcacg cagtttacta agttgcaaaa
    19201 tattggcaaa ttgtaactcc aagttatcaa agaattttgc cactcgagct ttgagcaact
    19261 cctcttcaaa catcaaggcg tgtccggtca ggatgcgggt aacgcccggg tttttgcgag
    19321 caaattctaa aatcacatat aaaattgctc gcaccgaatt ggcactgctg tcttggcgtt
    19381 tattggcgtg aatatagctg gtgagggtct gctcgatttt ctcgattaac gcttcaaaca
    19441 tcttggtttt acttgggaaa tagcgataca acgccccctc cgagaccccc accgcagcgg
    19501 ctaaacgctc ggtagtgata cgctgcattc cctcttcaga atttaacaag ctgattaaca
    19561 cttccaacac ttgttggcgg cgttccacca ctgatttttt cggcatttta attttaggtt
    19621 ctgtcattgc caatcctatt attgcttacc tgaatgccca aaaccgcctt cgccacggtc
    19681 ggtctgctca aactcttgca caatgttaaa acttgcctga accaccggca caaagactaa
    19741 ctgagcaata cggtcgccca cgttcacggt aaaatcagct tggctacggt tccaaagcga
    19801 cactttcagc tcgccttggt aatcgctatc gatcaagccg actaagttgc ctagcacgat
    19861 cccgttttta tgccctaaac ctgatcttgg taagatcacc gctgccaaat tcggatcggc
    19921 gatataaatt gaaatccccg ttgggatcaa caccgtttca cccgctttta ccgtcagcgg
    19981 ctcgttgagc atcgcccgta gatccaagcc tgccgagcct tcggtcgcat acgcaggtaa
    20041 tggaaattca ttgccgatac ggctatctaa aatttttaaa tcaatttgtt tcattctttt
    20101 tcctttttgt aaatttttaa aactatatta ttcagttata aaaggtttta attgtatcgt
    20161 tccaagtgga agttcagtca atatttccca gcttgtgttg tggtaaaatc gaaataaact
    20221 tgcaacgttt tcaacgtagc ttgaattaaa tttaccttct aatggcttta ttcctccagt
    20281 aaaatgaatt atatgtggaa tataatttgg cgaaattttt tgctgggcaa tatcatctaa
    20341 taaatgttct gtctgataat tata
//
LOCUS       DANLUQ010000028        20065 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.028, whole genome shotgun sequence.
ACCESSION   DANLUQ010000028 DANLUQ010000000
VERSION     DANLUQ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 20065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20065
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.028"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            342..1706
                     /locus_tag="PXB27_000813"
     CDS             342..1706
                     /locus_tag="PXB27_000813"
                     /EC_number="2.6.1.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005820549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HDL5345630.1"
                     /translation="MTIKTPIQAVLASNQYFLDRQDAMESNVRSYPRKLPFAYQKAQG
                     VWVTDVEGNEYLDFLAGAGTLALGHNHPKLIQAVKDVLDSGLPLHSLDITTPLKDAFT
                     EELLSFFPKDKYILQFTGPSGADANEAAIKLAKTYTGRGNVIAFSGGFHGMTHGALSL
                     TGNLSAKNAVQGLMPGVQFMPYPHEYRCPFGIGGEAGAKAVEHYFENFIEDVESGVVK
                     PAAVILEAIQGEGGVVPAPISFLQKVREVTQKHGILMIVDEVQAGFCRSGKMFAFEHA
                     GIEPDIVVMSKAIGGSLPLAVLAIKKEFDAWQPAGHTGTFRGNQLAMATGYVSLKIMR
                     EENLAKNAQERGEYLTNALRELSKELPCIGNVRGKGLMMGIDIVDERQPKDATGAYPR
                     DCDLAVAIQKYCFKNKLLLERGGRGGNVVRVLCAVNITQAECEEFIKRFKQSVAEAVK
                     AVRG"
     gene            1796..3331
                     /gene="ddc"
                     /locus_tag="PXB27_000814"
     CDS             1796..3331
                     /gene="ddc"
                     /locus_tag="PXB27_000814"
                     /EC_number="4.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2,4-diaminobutyrate decarboxylase"
                     /protein_id="HDL5345631.1"
                     /translation="MSNLEKHRQSLFCSDPKSIADYESAMNNAVKAVSDWLKEEKMYT
                     GGSIKELRAKINGFNPTQAGVGVQKSLEHLVEIFLKPSLKVHHPHSLAHLHCPTMVTS
                     QIAEVLINATNQSMDSWDQSPAGSIMEEHLIDWLRQKVGYGKGTSGVFTSGGTQSNLM
                     GVLLARDWAIANHWKKADGSEWSVQADGIPADAMQKVKVVCSENAHFSVQKNMAMMGM
                     GFQSVVTVPSNANAQMDVEALAKTLADLKAEGKIVACIVATAGTTDAGAIDDLKAIRK
                     LADEHQAWVHVDAAWGGALLLSKDFRHFLDGIELTDSITLDFHKHFFQSISCGAFLLK
                     DEANYRFIDYKADYLNSEYDEEHGVPNLVSKSLQTTRRFDALKLWFTVEALGEELYAS
                     MIDHGVYLSKEVEAYINATDGLEMLVPAQFASVLFRVVPQGYPAEFVDTLNQNVADEL
                     FARGEANIGVTKVGEHQSLKMTTLSPIATLENVRALLAQVLAEADRIKDSIVNGTYVP
                     PID"
     gene            3434..3796
                     /locus_tag="PXB27_000815"
     CDS             3434..3796
                     /locus_tag="PXB27_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NirD/YgiW/YdeI family stress tolerance protein"
                     /protein_id="HDL5345632.1"
                     /translation="MKKVLGLTLMAMALSACNSTASNGSSQANTGVAPANVISVKQAL
                     AAADDSYVTVEGTLLNKVGDETYLFKDATGQIRVEIDDEVWAGQHVGSEGNKVRLYGE
                     IDKGFNKTELDVKTLTVL"
     gene            complement(3886..4473)
                     /locus_tag="PXB27_000816"
     CDS             complement(3886..4473)
                     /locus_tag="PXB27_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL5345633.1"
                     /translation="MNHLIIYAHPNPSSFNHAILEKTIAASVPNKVVVRDLYQLNFKP
                     ILDWAEFQGTLSKHYEADVAIEHQYWLEADVITLIYPLWWMGFPAILKGYLDRVLSYD
                     FAYRNGEIESIGLLTGKKMQQFVTLGNSNEKYERKGFLAAFEHTLGKGLFEFCGIRDV
                     KMHYFGNIGLKETDYTAILQQITASCSTILKEQAV"
     gene            4649..5197
                     /gene="napF"
                     /locus_tag="PXB27_000817"
     CDS             4649..5197
                     /gene="napF"
                     /locus_tag="PXB27_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HDL5345634.1"
                     /translation="MTDYPNQSLPRRSFLRGQFLDSLKSQKVSQQGYEPIRPPWANLA
                     NFLEKCTACGNCIQACETQIIVKGAAGYPEIDFSRGECTFCEKCVHSCEAEVFRAVSE
                     QPWSHKIEIQAGCLLKLGTECRSCGDSCEMRVIRFRPSLGGIAEMVLNLESCNGCGAC
                     LSVCPTSAIKIQPLQTQANRDE"
     gene            5190..5456
                     /locus_tag="PXB27_000818"
     CDS             5190..5456
                     /locus_tag="PXB27_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HDL5345635.1"
                     /translation="MSEKLSEQENWYVCSLVVQAKPEKLEEVKAEILKIPHTEIHGEK
                     TDEGKLVVTIESNVHLALSDRIDQVRDIKGVIVVSLISNYIDEQ"
     gene            5495..7993
                     /gene="napA"
                     /locus_tag="PXB27_000819"
     CDS             5495..7993
                     /gene="napA"
                     /locus_tag="PXB27_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005566990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase catalytic subunit NapA"
                     /protein_id="HDL5345636.1"
                     /translation="MELNRRDFMKANAAVAAAAAAGIMIPVKNVQADDTGIRWDKAPC
                     RYCGTGCSVLVGTKDGKVVATQGDPDAEVNRGLNCIKGYFLSKMMYGADRMQEPMLRM
                     KDGKFDKNGDFTPVSWDQAFSIMAEKIKTTIKNDGPNAVGMFSSGQTTIFEGYAKVKL
                     WKGGFRSNTIDPNARHCMASAAVAFMRTFGMDEPMGCYNDIEKTDAFVLWGSNMAEMH
                     PILWSRISDRRLSDNKVKVVVMSTYEHRSFELADTPIIFKPHSDLAILNYIANYIIQN
                     DKVNWDFVNKHTKFKRGETNIGYGLRPEHKLQQNTNAKTAGKMYDSDFEEFKKIVAPY
                     TLEEAHRISGVPKDQLETLAKLYADPNQKLVSFWTMGFNQHTRGVWVNHMMYNVHLLT
                     GKIALEGCGPFSLTGQPSACGTAREVGTFIHRLPADMVVTNPKHREIVENSWKLPAGA
                     IPDVPGFHATAQSRALKDGKMKFLWQMCTNNMQGGPNINEEIFPGWRNPETFIVVSDP
                     YPSVSAVAADLILPTCMWVEKEGAYGNAERRTQFWRQQVKGPANARSDTWQLVEFSKY
                     FKVEEVWPEELLAKAPELRGKTLYEILYRNGKVDSYQVPTNIPGYMNDEADHFGYYIQ
                     KGLFEEYAEFGRGHGHDLADFDTYHKVRGLRWPVVKDEKTGEYKETLWRYREGYDPYV
                     KAGEEVAFYGNADKKAVILGVPYEPPAEAPDEEYDLWFCTGRVLEHWHTGTMTRRVPE
                     LHKAFPTNLVWMHPNDAKKRGLRHGDKVKVISRRGEYVSNLDTRGRNKCPEGLIYSTF
                     FDAGQLINKVTLDATDPISFETDFKKCAVKVVKA"
     gene            8082..8987
                     /gene="napG"
                     /locus_tag="PXB27_000820"
     CDS             8082..8987
                     /gene="napG"
                     /locus_tag="PXB27_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HDL5345637.1"
                     /translation="MKMDPNRRQFLKNATRTAAGVCGVGMILGLQQQQSFARQGVALR
                     PPGALPEQDFLSACTRCGQCVQACPYDMLHLASLISPVEAGTPYFIARDKPCEMCLDI
                     PCMNACPTGALSEELKDIDDARMGLAVLLDHETCLNWQGLRCDVCYRVCPLIDKAITL
                     EKVHNDRTSIHAKLIPTVHSDACTGCGKCEQACVLEEAAIKVLPMDLAKGLLGRHYRL
                     GWQEKQNAGKSLIEDVHPEGLRPAYDARMPEGLHEPVYQHMEVKPDVKVATPSRATQN
                     YVPNSTTVEAPEHFPDLDLNLKGVK"
     gene            8987..9865
                     /gene="napH"
                     /locus_tag="PXB27_000821"
     CDS             8987..9865
                     /gene="napH"
                     /locus_tag="PXB27_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HDL5345638.1"
                     /translation="MAANSPKNAGLEARQKLGWWHAYRFLILRRLSQLSIILMFLSGP
                     LWNVWILKGNYSGSLLLDTVPMTDPLMTAEVLATGYRPEWTVLVGALIMVAFYGIVGS
                     KLFCSWVCPMNMVTDTAAWLRRKLGIRQTAKIPRNLRYVILAVILVGSAISGTLLWEW
                     INPVAALGRVFVFGLGATLWLVLVVFLFDLLVAEHGWCGHLCPIGATYALIGAKSLIK
                     VKVVDRNRCDNCMDCYNVCPEPQVLRVPLHGKPEESTVILSKDCITCGRCIDVCAENV
                     FAFGMRFQGSIDVKNI"
     gene            9911..10339
                     /locus_tag="PXB27_000822"
     CDS             9911..10339
                     /locus_tag="PXB27_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HDL5345639.1"
                     /translation="MRKYLAFILAVMSGFAMAEAPKLSGSIDETTESVAPGFHNPQKD
                     VGTIPTTFPFQPPLVPHSIRGLQVSKNVNHCLGCHAIEPSKTTGATRLPPSHFTDRDG
                     KVYENESPRRYFCLQCHVQQTDVNPIIQNKFESVRESQAK"
     gene            10350..10958
                     /locus_tag="PXB27_000823"
     CDS             10350..10958
                     /locus_tag="PXB27_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HDL5345640.1"
                     /translation="MLRRFCNWLRSPSKMAIGAVILISALGGIFAWSGFNAGLAMTNT
                     EEFCSDCHMNDVVPEYRASAHYSNRSGVKAICSDCHVPHEFFPKWQRKIIAAKEVYAH
                     FTGKVDTKEKFEAHRGEMAEREWARMKANSSQECRNCHNFDDMDFTQQKNIAQQMHAL
                     AQEQGKTCIDCHKGIAHNLPHMEAIQKNFIPDDLLKKAEEKK"
     gene            complement(11020..11862)
                     /gene="cpdA"
                     /locus_tag="PXB27_000824"
     CDS             complement(11020..11862)
                     /gene="cpdA"
                     /locus_tag="PXB27_000824"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HDL5345641.1"
                     /translation="MDAKRMHEYIKLPVTGVIKLLQITDPHLFSNPEETLLNVKTVKS
                     FSAVIEQINKQAKQYFDLVLATGDLIQDNNIAGYHYFAQITNSLNSPIVWLEGNHDVQ
                     PSMSEILAQYKHILPHKQILLGEQWQLLMLNTQVVGMPYGELSYSQLKWLEEKLAQYP
                     QRFTLVALHHNILPTNSAWLDQHSLKNSNELSEIFAKFKNVKAIVHGHIHQQVDDIWH
                     GIPIYATPSTCIQFKPNCDQFTLDLQPQGWREIELHENGTIETVVKRLNSNDFLPNVN
                     AIGY"
     gene            12152..12286
                     /locus_tag="PXB27_000825"
     CDS             12152..12286
                     /locus_tag="PXB27_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/trimethylamine N-oxide reductase
                     NapE/TorE"
                     /protein_id="HDL5345642.1"
                     /translation="MRSELLKTLLLSGLILPLLMAFAVGAYGFIVWMLQLFVIGLPGA
                     "
     gene            12290..13453
                     /gene="torC"
                     /locus_tag="PXB27_000826"
     CDS             12290..13453
                     /gene="torC"
                     /locus_tag="PXB27_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HDL5345643.1"
                     /translation="MLGSIKALFTRPATKIGLGVLVGIGFIAGAISWHQFNKAMDHTS
                     TEEFCVSCHSMQQPLEELKKTAHWSNKSGVTATCSSCHLPHDYTEKYARKVQASREAW
                     AELSGKYKEEGSFEKHRLEMAEREWARFAANGSKECKACHSYERMNFDKMGEAARKAM
                     KPAAEKDQSCMDCHKGIAHHLPKADENALKSKFDQFVNKSPNQGKSYYTKGNAELFLD
                     EGLSQAIGQLETAVPVAFVQAGKNADLVELTMWRKEKGFGRIWYHDFGKNITDAVLTK
                     EFMANQPKFEVLETKEDPITGLNWQKVKLQAWIGKAQLLDDVTPIWANAENQYKTQCS
                     TCHRQPDVAHFDSNSWIGLFNGMVGFTNMDKQTGKEVLRYLQMHASDSEEAKH"
     gene            13553..16018
                     /gene="torA"
                     /locus_tag="PXB27_000827"
     CDS             13553..16018
                     /gene="torA"
                     /locus_tag="PXB27_000827"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HDL5345644.1"
                     /translation="MQQSRRQFLKNMSVMAAAVTMPNFLVPRNVFASEDLSQWKVSGS
                     HWGAMRAKIENGRVAEIKPFEFDKHPTEMLNGIKGLIYSDSRIRYPMVRLDWLKNRHN
                     SNTAQRGDNRFVRVTWDEALDLFYQELERVQKEYGPWALHTANVGWRSTGQFHSCGNH
                     MIRAIAMHGNSVGTAGDYSTGAGQTILPYVLGSTEVYSQGTSWEIILKESENIIFWAS
                     DPVKNLQVGWNCETHEAYAYLEKLKEKVAAKGVNVICVDPVKSKTQNFLGCEQQYINP
                     QTDVPFMLALAHTLYTENLYDKKFLDMYTVGFEKFLPYLLGETEDKVAKTPEWAAEIC
                     GISADKIREFARMLAGKRTQFIFGWAIQRQQHGEQPYWMGAVLASMLGQIGLEGGGIS
                     YAHHYSSIGIPESGAAMPGAFPLNIDEGQTPKYDNKDYKGYSDTIPVARMTDSLLHAG
                     ETIDYNGKKVTYAPFKMAIFTGCNQWHRHSERNKMKQAFQKLETIVSINYSWTATCRF
                     SDIVLPACTPFERNDIDAYGSYSNRGVIAMHKLVDPLYDSRSDFEIFRDLCRRFGKEK
                     EYSRGMDEMQWVEKLYKDCRRENRGKFEMPEFAEFWQKGYVLFPEGKPWVRHADFRED
                     PELHALGTPSGFIEIYSNKIASFGYDDCKGHPMWFEKAERSHGGKNSDKFPYWLQSVH
                     PDKRLHSQLCESKELRETYSVQGREPLYINPQDAAKHGIQDGDLVRVFNDRGQAIVGA
                     VLSDNFPTGVVRLQEGAWYSPLDESVGAIDTYGDPNTMTLDIGTSKLAQAVSANTCLV
                     NIEKFIGNAPAPNGFNGAIEVTA"
     gene            16018..16608
                     /gene="torD"
                     /locus_tag="PXB27_000828"
     CDS             16018..16608
                     /gene="torD"
                     /locus_tag="PXB27_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HDL5345645.1"
                     /translation="MTLAISREERQFIYGWFNAMLARELSDEQLNALQAGEFDDFFAY
                     LSELGFAEQVAKFQAEVTACRQLEFPRLELAADFAQLFLLEGNLSAIPYASAYLSGEA
                     LAKNLAEMDRLLGEFGLQVNRETKEPSDHLCVYLTLFDRLLAEKPLETQQQFLQNQLM
                     SWLPAWFAKAKTVKTNTGVYPALLRLFEQVLLKEQR"
     gene            16748..17437
                     /locus_tag="PXB27_000829"
     CDS             16748..17437
                     /locus_tag="PXB27_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL5345646.1"
                     /translation="MQNNKTTYLNWYDLCVLTLILFGEAIYTSTQQYFITTEMQGNFE
                     ENLTFSNEQNYWALLTQLALLLIAMGYLKWRNFDFSRWVIRPSWLAFFSGIGLFILLS
                     LAMDLYFETLYGVYNLVYPEPFWAVLREVNISTVLYAILNGFYEEIFFLGICLAVKPE
                     YLKWAVIYSLIIRVSFHTYQGMESALGLGIWLGLIIYLLYSRSKQKNLFPFWVAHSIA
                     DILGLSLLAYF"
     gene            17595..18455
                     /locus_tag="PXB27_000830"
     CDS             17595..18455
                     /locus_tag="PXB27_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HDL5345647.1"
                     /translation="MKSIKKLTLATLILVGLTACGSGGSGSSSNNAKSKPQVELKKTE
                     EDKKTEEDKKTEEDKKTEEDKKTEEDKKTEEDKKTEEDKKAEEAKKIAEAKGIGNKEY
                     KDGLNELEEKNETGKNGEEIRTHGYLYNSHYSVVTAKMKQTLQENGRQMEPTVEVKGL
                     KTENLPTEGKATYKGEAFDSHGNNSNSVVGGELIYNVDFSSRTGSGLVKNVQGGSIEL
                     AQGEIKNDSIIASAHQKYNDQAVGNGSYNIQFFGPNAEEIGGKIELNGEGEGSMKQML
                     GIAGTREEQK"
     gene            18504..19898
                     /locus_tag="PXB27_000831"
     CDS             18504..19898
                     /locus_tag="PXB27_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HDL5345648.1"
                     /translation="MYKKHFLFLLVSPLVMAETVEFQQDPKEFDGQRQAVKFEQEIAK
                     KDLKLTAYLEEDNSLKGIEQHINQAILRQDWAKLERLLGDYAKQEQFDPLLLDYALAA
                     LAYAKKDYRSAAFYYQQLLKKQPDLTYPRFDLALILKEDYRYREAEQEFKQLKTDDLR
                     NAVNSALAQIEEEESWKPDFYLQYTQTNNVNNASSSPTVNVNGRIFIKESDSLPQSAK
                     GVRYGVGLSKMRNIGGNHFVGVDLGYNGVYYWNNHDYSEQSVSLTPKYSYRTATYRIG
                     VSPFIEQNWLGSSRYNYQFGSSINAVKLFERGLISGSFSHLQKRYSEQHIADRYNGYR
                     NQLTLGTQWQAVKNWRFFANLNASREITREKAQSSNKWLATIGVIYQTPSWASSVSVG
                     YGKRYFADKHYLFGYKRQDKEYQANVAVWNRSWHWQGIVPKLNFRYQKIDSNIADFYS
                     RSNKEVFMTLEKLF"
BASE COUNT         5983 a         3979 c         4672 g         5431 t
ORIGIN      
        1 ttaatcttat tgatttaatt taatcaattt taaggtggta aattggatag ggtatgaaag
       61 tgaaaggcat ttattgctag ataaaagctt cccttttcaa taaaaaagag tatcatacgc
      121 tctcttaaac gtaaccctac atttaagtct gtttccaacc tatcccaatt tctttattcc
      181 ttaggggcat atagggtaaa aacaaaaaag ctgaaacgct ttttttaata agtagcgagt
      241 caaatgcccc atggcatcta ttcttttagg aatagaaagc ttaggctttc cgcaaggcac
      301 ccgacttatt tttttcacct cttgtagaag gtttattttc aatgacaatt aaaactccta
      361 ttcaagccgt tcttgcttca aaccaatatt ttttagatcg ccaagatgca atggaatcaa
      421 acgtgcgtag ctacccacgt aaattacctt ttgcttacca aaaagcacaa ggcgtttggg
      481 taactgatgt tgaaggcaat gaatacctcg atttcctagc aggtgcagga acgcttgcat
      541 taggtcataa ccaccctaaa ttaatccaag cagttaaaga tgtattagac agtggtttac
      601 ctttacactc gttagatatt acgactccgt taaaagatgc ttttacggaa gaattactct
      661 ctttctttcc aaaagataaa tatatcctcc aatttactgg cccgtctggt gcagatgcta
      721 acgaagcggc aattaaatta gccaaaacct acactggtcg tggcaacgta attgctttct
      781 ccggtggttt ccacggaatg acccatggtg cgttatcgct caccggtaat ttaagtgcga
      841 aaaatgcagt acaaggctta atgccgggcg tacagtttat gccttatccg cacgaatacc
      901 gctgtccgtt tggcattggt ggcgaagccg gtgcaaaagc agttgaacat tattttgaga
      961 actttattga agatgttgaa agtggtgtgg taaaaccggc agcggtgatc cttgaagcca
     1021 ttcaaggtga aggcggtgtg gttccggctc caatcagctt cttacaaaaa gtgcgtgaag
     1081 tcactcaaaa acacggcatc ttaatgattg tggacgaagt tcaagctggt ttctgccgtt
     1141 cagggaaaat gtttgccttt gaacacgcag gcattgagcc ggatatcgta gtaatgtcaa
     1201 aagcgattgg tggtagctta ccgcttgcgg tattagcgat caaaaaagag tttgacgctt
     1261 ggcaaccggc aggtcatacc ggtacattcc gtggtaacca attagcaatg gcaaccggct
     1321 atgtatcgct taaaattatg cgtgaagaga atttagcgaa aaacgcacaa gagcgtggcg
     1381 aatacttaac taacgcatta cgtgaattaa gcaaagaatt accttgtatt ggcaatgtgc
     1441 gtggtaaagg cttaatgatg ggaattgata ttgttgatga acgtcagcca aaagatgcaa
     1501 caggtgcata cccgcgtgat tgcgatttag ccgttgcaat tcaaaaatac tgctttaaaa
     1561 acaaattgtt attagagcgt ggcggacgtg gcggtaatgt ggtgcgtgtt ctttgtgccg
     1621 tgaatattac ccaagcagaa tgtgaagagt ttattaaacg ctttaaacaa tctgttgctg
     1681 aagcagtgaa agcggtgcgt ggttaatagt tagaatggta ggagcgaaac atttttcgcc
     1741 ctaatgtaaa aaggggcgaa tatttattcg cccctacacc aataggatag aaataatgtc
     1801 aaacctagaa aaacacagac aatccctgtt ttgtagcgat ccaaaatcga ttgcggatta
     1861 tgaatcggca atgaataacg ctgtgaaagc ggtttcagac tggctgaaag aagaaaaaat
     1921 gtacaccggt ggctcaatta aagagttgcg tgccaaaatc aacggtttta acccaaccca
     1981 agctggtgtg ggcgtgcaaa aatcactgga gcatttggtg gaaattttct taaagccaag
     2041 cctcaaagtt caccaccctc attctttggc ccacttacat tgcccgacaa tggtaaccag
     2101 ccaaattgca gaagtgttaa ttaatgccac caaccaatca atggactctt gggatcaaag
     2161 cccggcaggt tcgattatgg aagagcattt aattgactgg ctacgtcaaa aagtcggcta
     2221 tggcaaagga acttcaggag tctttacctc aggtggtacg caatcaaacc tgatgggcgt
     2281 attattggct cgtgactggg cgatagcgaa ccattggaaa aaggcagacg gttcagagtg
     2341 gtcggtgcaa gccgacggta ttcctgccga tgcaatgcaa aaagtgaaag tggtttgttc
     2401 cgaaaacgct cacttctcgg tgcagaaaaa tatggcaatg atgggaatgg gtttccaatc
     2461 ggtagtgact gtaccgtcaa atgccaatgc acaaatggac gttgaagctc ttgcgaaaac
     2521 gttggcggat ttaaaagccg aaggcaaaat tgtggcttgt atcgtcgcaa ccgcagggac
     2581 gaccgatgcc ggtgcgattg atgatttaaa agcgatccgt aaattagccg atgaacatca
     2641 ggcttgggtt cacgttgatg ctgcttgggg tggtgcatta ttactgtcga aagatttccg
     2701 ccacttctta gacggcattg aattaaccga ttccattacc cttgatttcc acaagcattt
     2761 cttccaatca atttcgtgtg gggcattctt gctaaaagat gaagcgaatt accgctttat
     2821 tgattacaag gcagactacc tgaattcaga atatgacgaa gaacacggcg tgccgaattt
     2881 agtgtcaaaa tcattgcaaa ctacccgccg ttttgatgcg ttaaaattat ggtttaccgt
     2941 tgaagccttg ggtgaggagc tatacgcttc aatgatcgac cacggggttt atctttctaa
     3001 agaagtggaa gcctacatta atgccaccga tggcttggaa atgttagtgc cggcacaatt
     3061 tgcctcagtc ttattccgtg tcgtaccaca aggttacccg gcagaatttg tggatacctt
     3121 aaaccaaaat gtggcagatg agttatttgc tcgtggcgaa gctaacattg gtgtgaccaa
     3181 agtcggtgag catcaatcac tgaaaatgac tactttaagc ccgattgcaa cgcttgaaaa
     3241 cgtaagagca ttattagctc aggtgttggc agaagcagat cggattaaag attcgattgt
     3301 aaatggaact tatgttccgc caattgatta aactgcgtta aaaataaaaa ggtgttttcg
     3361 acatttattt gtgttttaat gtttgcattc ggtagtcttt atcgaatgca aattttttta
     3421 acaaggagtt tttatgaaaa aagtcctggg tttaacattg atggcaatgg cgttatcggc
     3481 gtgtaattca actgcttcca acggttcttc acaagcaaat acaggtgttg caccggcgaa
     3541 tgtgattagc gtgaaacagg cgttagcggc agcagatgac agctacgtga cggtagaagg
     3601 cacactctta aacaaagtcg gtgacgaaac ttacttattt aaagatgcga cagggcaaat
     3661 ccgtgttgag attgacgatg aagtatgggc aggtcagcac gttggtagcg aaggcaataa
     3721 agtccgttta tacggagaga ttgacaaagg tttcaataaa accgagttag atgttaaaac
     3781 actcactgtt ctgtagaaga tcaaacctta atttggttcc ccgccttgtt tggcggggtt
     3841 tttacgttaa ttaatcaaac catccttttg caaaaaaacg caaaattata ccgcttgttc
     3901 cttcaatatt gtgctacaag atgctgtgat ttgctgcaaa atagcggtgt aatcagtctc
     3961 ttttaaacca atattaccga aatagtgcat ttttacatca cgaatcccac agaactcaaa
     4021 cagccctttt cccagcgtat gttcaaatgc tgctaaaaag ccttttcgtt catatttttc
     4081 attggaatta ccgagcgtta caaattgttg catttttttg ccggtcagca agccgatact
     4141 ttcaatttcc ccgttacgat aggcaaaatc ataacttaac accctgtcta aatagccttt
     4201 taaaattgcc ggaaagccca tccaccaaag cgggtaaatt aacgtaatca catcagcttc
     4261 aagccaatat tgatgctcaa ttgccacatc tgcttcatag tgtttactga gtgtgccttg
     4321 aaattccgcc cagtctaata tcggcttgaa gttgagttgg tagagatctc gtacaaccac
     4381 cttatttgga acggaagctg ctatcgtttt ttccaaaatc gcatggttaa agctggaggg
     4441 attcgggtgt gcgtaaataa tcaggtgatt catagcgttt tcctttactg tgtcgaggca
     4501 gctactagaa gattttagcg aaactttctt tgatgaaaat caatattttg gcgaatatac
     4561 cggcaaatac cccgtttgag gaatgaacgg ttttaagtga acattagata atttagccta
     4621 ttatagtgat gagtaaaaag gtgttttctt gactgattat cctaatcaat cacttcctcg
     4681 ccgtagcttt ttgcgagggc aatttttaga ctcgttaaaa agccaaaaag tcagtcagca
     4741 agggtatgag ccgattcgtc cgccttgggc taatttagca aattttttag aaaaatgcac
     4801 cgcttgcggt aactgcattc aggcttgcga aacccagatt attgtaaaag gtgctgcggg
     4861 ctaccctgaa attgatttta gccgtggtga atgcactttt tgtgaaaaat gtgtgcattc
     4921 ttgcgaggca gaggtattcc gagcagtttc agaacagcct tggagccata aaattgagat
     4981 tcaagccggc tgtttattaa agctcggaac ggaatgccga agctgtggcg atagctgtga
     5041 gatgagagtg attcgtttcc gcccgagttt aggtggcatt gccgaaatgg tattaaattt
     5101 ggaaagctgt aatggctgtg gggcttgttt gagcgtttgt ccgacaagtg cgataaaaat
     5161 tcagccatta caaacacagg caaacagaga tgagtgaaaa attatccgaa caggaaaact
     5221 ggtatgtgtg cagtctcgtg gtgcaagcaa aaccggaaaa attggaagaa gtaaaagccg
     5281 agattctgaa gattcctcat accgaaattc acggtgagaa aacagatgaa ggcaaattgg
     5341 tcgtgacgat tgaaagcaat gtgcatttag ccctttccga tcgaatcgat caagtaagag
     5401 atattaaagg cgtgattgtg gtgtctttaa tttctaatta cattgatgaa cagtgattaa
     5461 tgattatttt tgaagccaaa cgtgggacat agttatggaa ctcaatcgta gagattttat
     5521 gaaagcgaac gccgctgtgg cagctgctgc agccgcagga attatgattc ctgtgaaaaa
     5581 cgttcaggca gatgacaccg gtattcgttg ggataaagcc ccttgtcgtt actgtggtac
     5641 aggttgtagc gtgctagtcg gtactaaaga cggtaaagta gtggcaaccc aaggtgaccc
     5701 ggatgcagaa gtaaaccgtg gtttaaactg tattaaaggt tatttcttat ccaaaatgat
     5761 gtacggtgca gaccgtatgc aagagccaat gttgcgtatg aaagacggta agttcgacaa
     5821 gaacggcgat ttcacgccgg tttcttggga tcaagccttt agcattatgg cggagaagat
     5881 taaaaccact attaaaaatg acggcccgaa tgctgtaggt atgttctcat caggtcaaac
     5941 aaccattttt gaaggttatg cgaaagtgaa actttggaaa ggtggtttcc gttcaaatac
     6001 gattgacccg aatgcccgcc actgtatggc gtccgctgcg gttgccttta tgcgtacctt
     6061 tggtatggac gagccgatgg gctgctataa cgacatcgaa aaaaccgatg cgttcgtgct
     6121 ttggggttca aatatggcag aaatgcaccc gattttatgg tctcgtattt ctgatcgccg
     6181 tttatcagac aataaagtga aagtggtggt aatgtccact tacgaacacc gttcgtttga
     6241 attagccgat acaccgatta tcttcaaacc acactcagat ttagcaattt taaactacat
     6301 tgcgaactac attatccaaa acgataaagt aaattgggat tttgttaaca aacacaccaa
     6361 atttaaacgg ggtgaaacca atatcggtta cggtttacgt cctgaacaca aattacaaca
     6421 aaataccaat gcgaaaactg ccggtaaaat gtatgacagc gattttgaag agtttaagaa
     6481 aatcgttgcc ccttatacat tagaagaagc tcaccgtatt tcaggcgtgc cgaaagatca
     6541 gcttgaaaca ttagcgaaat tatatgcaga tcctaaccaa aaattagtgt cgttctggac
     6601 gatgggcttt aaccaacata cccgtggtgt gtgggtaaac catatgatgt acaacgtcca
     6661 tttattaacc ggtaagatcg cattagaagg ttgtgggccg ttctcactca cgggtcagcc
     6721 gtccgcttgt ggtactgccc gtgaagtggg tacatttatt caccgcttac cggcagatat
     6781 ggtggtcaca aatccgaaac accgtgagat tgtagagaat tcttggaaat taccggctgg
     6841 agcaattcct gatgtaccgg gctttcacgc tacagctcaa agccgtgcct taaaagacgg
     6901 caaaatgaaa ttcttatggc agatgtgtac caacaatatg cagggcggcc cgaatatcaa
     6961 tgaagagatc ttcccgggct ggcgtaatcc tgaaaccttt attgtggttt cagatccgta
     7021 cccatctgtt tcagcagtgg ctgctgactt aattcttcca acctgtatgt gggtggaaaa
     7081 agaaggggct tacggtaacg cagaacgccg tacccaattc tggcgtcagc aagttaaagg
     7141 gccggcaaat gcccgttcag atacttggca gttagtagag ttctctaaat acttcaaagt
     7201 agaagaagta tggccggaag agttattagc taaagcaccg gaattgcgtg gtaaaactct
     7261 atacgaaatc ctttaccgca acggcaaagt agatagctac caagtgccaa ctaacattcc
     7321 gggctatatg aatgacgaag cagatcactt cggttactac attcaaaaag gcttatttga
     7381 agagtatgcg gaatttggcc gtggtcacgg tcacgattta gccgactttg acacctacca
     7441 caaagtacgt ggcttacgtt ggccggtggt aaaagacgag aaaaccggcg aatacaaaga
     7501 aactttatgg cgttaccgtg aaggctatga cccatacgtt aaagccggtg aagaagtggc
     7561 attctatggc aatgcggaca aaaaagcagt gattttaggt gtgccatacg aaccacctgc
     7621 ggaagcaccg gacgaagaat atgacttatg gttctgtacc ggtcgtgtac tagaacattg
     7681 gcacaccggt acgatgaccc gccgtgtgcc ggagttacat aaagcattcc caaccaactt
     7741 ggtttggatg cacccgaatg atgcgaaaaa acgaggctta cgtcacggtg ataaagtgaa
     7801 agtgatttca cgccgtggtg aatatgtatc gaacttagat acccgtggac gtaataaatg
     7861 tccggaaggt ttgatttact cgactttctt cgatgccggt cagttaatca acaaagtgac
     7921 acttgatgcg actgatccaa tttcatttga aaccgacttc aaaaaatgtg cggttaaagt
     7981 ggtgaaagct taattgtagg ggcaaattat ttgcctcaga caagcggtca aatttgcaaa
     8041 tttttttaca aaaacgaccg cttacctaag aggaacccac aatgaaaatg gatccaaatc
     8101 gtcgccagtt tttaaaaaat gcaacccgta ccgcagccgg tgtttgcggt gtaggaatga
     8161 ttttagggct acagcaacaa caaagttttg cccgtcaagg ggtagcacta cgcccaccgg
     8221 gtgcattgcc ggagcaagat ttcctgagtg cttgcacccg ttgcggacaa tgtgtgcagg
     8281 cttgtcctta cgatatgttg catttggcat cgcttatttc accagtggaa gccggcacgc
     8341 catactttat tgcacgggat aaaccttgcg aaatgtgctt ggatattccc tgtatgaatg
     8401 cttgcccaac cggggcatta agcgaagagc taaaagacat tgacgatgca cgaatggggc
     8461 ttgcggtgtt gctcgaccac gaaacttgtc ttaactggca aggcttgcgt tgtgacgtgt
     8521 gttaccgtgt ttgtccgtta atcgacaaag cgattactct tgagaaagtg cataacgacc
     8581 gtacctcgat tcacgccaaa ctgattccga ccgtgcattc tgatgcttgc accggttgcg
     8641 gtaaatgtga acaggcttgc gtattggaag aggcagcgat caaagtgtta ccgatggatt
     8701 tagccaaagg cttgctcggt cgtcactatc gtttaggctg gcaagagaaa caaaatgccg
     8761 gtaaatcgct gattgaagat gttcacccag agggtttacg cccagcttat gatgccagaa
     8821 tgccggaagg cttacacgag ccggtgtatc agcatatgga agtaaaacct gatgtgaaag
     8881 tggctacgcc aagtcgggcg acacaaaact atgtgccgaa ctctacgacg gtggaagctc
     8941 cggagcattt ccctgattta gacttgaatc tcaagggggt gaaataatgg cagccaattc
     9001 tccaaaaaat gcgggcttag aggctcgtca gaaactaggt tggtggcacg cttaccgctt
     9061 tttgatttta agacgcttaa gccaactcag cattattttg atgtttttaa gcggcccact
     9121 ttggaatgtc tggattttaa aaggaaacta cagcggtagc ttattactag ataccgtgcc
     9181 gatgacagac ccattaatga ccgctgaagt gctggcaacc ggctatcgcc ctgaatggac
     9241 ggtattggtt ggggcgttaa ttatggtggc gttttacggc attgtcggca gcaagctgtt
     9301 ttgtagttgg gtctgcccga tgaatatggt aacagatact gctgcttggt taagacgcaa
     9361 actcggtatt cgccaaactg ctaaaattcc acgcaattta cgctatgtga ttttggcggt
     9421 gattttagtc ggtagtgcga tttccggaac actattgtgg gagtggatca acccggttgc
     9481 ggctctcggt cgggtgtttg tattcggttt aggggcaacg ctttggctgg ttttagtggt
     9541 gttcttgttt gacttattgg tggcagaaca tggctggtgt ggtcatcttt gcccgattgg
     9601 tgcaacgtat gccttaattg gggcgaaaag cctgattaaa gtcaaagtgg tagatcgtaa
     9661 ccgttgcgat aattgtatgg attgttacaa cgtttgtccg gaacctcaag tgttgcgtgt
     9721 gccgctacac ggtaaaccgg aggaaagcac ggttatctta tctaaagatt gcattacctg
     9781 cggacgttgt attgacgttt gtgccgaaaa tgtatttgcc ttcggaatgc gttttcaagg
     9841 cagtattgat gtgaaaaata tttaattttt atctttttga aaacaagaat tttaatggag
     9901 tgaacctcaa atgagaaaat atctcgcctt cattttagcc gtgatgtccg gctttgcaat
     9961 ggcggaagcc cctaagttat ccggatcaat tgatgagaca accgaaagtg ttgcaccggg
    10021 atttcataac ccacaaaaag atgtgggcac gattccaacc actttccctt tccaaccacc
    10081 gcttgttcca catagtattc gtggcttaca agtgagcaaa aacgtaaacc attgtttagg
    10141 ttgccacgct attgaaccat ctaaaaccac aggtgcgacc cgtttaccgc caagccattt
    10201 taccgaccgt gatggcaagg tgtacgaaaa cgaatctcct cgtcgttatt tctgtttgca
    10261 atgccacgtg caacaaactg atgtaaaccc gattattcaa aataaatttg aatctgtacg
    10321 tgaatctcaa gctaaataag gagtaagtaa tgttgagaag attttgtaac tggttacgta
    10381 gcccaagcaa aatggcaatt ggtgctgtaa tccttatttc tgcattaggt ggtattttcg
    10441 cttggagtgg ttttaatgca gggttagcaa tgacaaatac tgaagagttt tgttcagatt
    10501 gccatatgaa cgatgttgtg ccggaatacc gtgcctctgc tcactattca aaccgcagcg
    10561 gtgtgaaagc gatttgttca gattgccacg taccgcacga attctttcca aaatggcaac
    10621 gtaaaatcat agccgcaaaa gaagtttatg cacactttac cggtaaagta gataccaaag
    10681 agaaatttga agctcatcgt ggcgaaatgg cagaacgtga atgggcacgt atgaaagcga
    10741 acagctctca agagtgccgt aactgtcata attttgatga tatggacttc actcaacaaa
    10801 aaaacattgc gcagcaaatg cacgcattgg cacaagagca aggtaaaacc tgtattgact
    10861 gccacaaagg gattgcacac aatctcccac atatggaagc gatccagaaa aactttatcc
    10921 ctgatgattt gctgaaaaaa gcagaagaga agaaataact taacgtgttt caatagaaaa
    10981 ccctcaattt ccattgaggg tttcttttta gtagccaagt tagtaaccaa tggcattgac
    11041 atttggtaaa aaatcgttgg aatttaaccg cttgacgacc gtttctatcg tgccgttttc
    11101 gtgcaattca atttcacgcc agccttgtgg ttgtaagtct aaggtaaact ggtcgcaatt
    11161 cggcttaaat tgaatgcaag tagatggggt ggcataaatc ggaataccat gccaaatatc
    11221 atcaacctgc tgatgaatat gaccatgcac aatcgctttt acattcttaa attttgcgaa
    11281 aatctcggat aattcattgc tgttttttaa gctatgctga tccagccacg ctgagtttgt
    11341 cggcaaaata ttgtggtgca gagcaaccaa ggtaaaccgt tgcggatatt gtgccaattt
    11401 ttcttctaac catttaagct gtgagtaaga gagttcgcca taaggcatac ctaccacctg
    11461 ggtatttagc atcagaagct gccattgctc accgagtaaa atttgtttat ggggaaggat
    11521 atgtttatat tgcgccaaaa tttctgacat actcggctga acatcgtgat tcccctctag
    11581 ccacactatc ggcgagttta gcgaattagt tatctgtgca aaatagtgat aaccagcaat
    11641 attgttatct tgaatcaaat cacctgtggc aagaactaga tcaaaatatt gcttcgcttg
    11701 tttgtttatt tgctcaataa ctgccgaaaa actttttact gttttgacat taagtagagt
    11761 ttcctccgga ttggagaaaa ggtgagggtc agttatttgt aataacttaa ttacccctgt
    11821 tacgggtaac ttaatatatt cgtgcattcg ttttgcatcc attcaaattc tagtaatttt
    11881 taactggtga gattctatca gtaagctaaa agtaaaaatg tggactatgc cgcatttttg
    11941 aaaaaaagtg agattaatca aaaaaaattt attattttcg atagctaaga caatggtaag
    12001 tttgattaat actttcatat tgtaatagat gatttttata ctgtttggct gtttctagaa
    12061 caacttcttt atatttttga ttgatatcaa ggcaaattgt ttctgaagat aaaataatta
    12121 acacttatta gttaataaaa aaggagttga aatgcgttca gaactattaa aaacgctttt
    12181 gctatcaggg ctgattttac cgctattgat ggcgtttgcc gtaggagctt acggctttat
    12241 tgtgtggatg ttacaactgt ttgttattgg tttaccggga gcgtagaaaa tgttaggttc
    12301 aatcaaagca ttatttactc ggcctgcaac caaaatcggg ttgggagtgt tagttggtat
    12361 cggttttatt gccggagcaa ttagctggca tcaatttaat aaagcgatgg atcataccag
    12421 cactgaagag ttttgcgtga gttgccactc aatgcagcag ccgttagagg agcttaaaaa
    12481 aaccgcccac tggtcgaata aaagcggggt aacggcaacc tgttcaagct gccacttacc
    12541 gcacgattat accgaaaaat atgcccgcaa agtgcaggca agccgtgagg cgtgggcaga
    12601 attatccggc aaatataaag aagaaggctc atttgaaaaa caccgcttag aaatggcaga
    12661 gcgtgagtgg gcaagatttg ccgccaatgg ttcaaaagag tgtaaggctt gccacagcta
    12721 tgagcgaatg aattttgata aaatgggcga agcagctcgt aaggcaatga aaccggctgc
    12781 ggagaaagat caaagttgta tggattgcca caaaggcatt gctcaccact taccgaaagc
    12841 cgatgaaaat gcactgaaat cgaagtttga tcagtttgtg aataaatccc caaaccaagg
    12901 caaatcttac tacaccaaag ggaatgccga attatttcta gacgaaggtt taagccaagc
    12961 tatcgggcaa ttagaaaccg ccgtgccggt ggcatttgtg caagcgggca aaaatgcgga
    13021 tttggttgag ctgacaatgt ggcgtaaaga gaaaggtttc ggtcgcattt ggtatcacga
    13081 tttcgggaaa aacattaccg atgcggttct aaccaaagag tttatggcaa atcagccgaa
    13141 atttgaggtg ttggaaacca aagaagatcc gattaccggc ttgaattggc aaaaagtgaa
    13201 actacaagcg tggattggca aagctcaatt attagacgat gttaccccaa tctgggcgaa
    13261 tgcagaaaac caatacaaaa cccaatgtag cacttgccac agacagcctg atgtcgccca
    13321 cttcgattcc aacagctgga tcggcttatt caacggaatg gtcggcttta ccaatatgga
    13381 taaacagacc ggcaaagagg tgctacgtta tttacaaatg cacgcctccg atagtgaaga
    13441 agcgaagcat taataatatt actcccctct ttactaaaga ggggaattaa tcgagcgttg
    13501 aataacattc tctgtactag ctcacgtttt tacaaataaa gaaggacaag atatgcaaca
    13561 atctcgccgt caatttttaa aaaatatgtc tgtgatggct gccgctgtca caatgccgaa
    13621 tttcctcgtg cctcgcaatg tgtttgcgag tgaagacctc agtcaatgga aagtttccgg
    13681 ctctcactgg ggggcaatgc gtgccaaaat tgagaacgga cgagtggcag aaatcaaacc
    13741 gttcgagttt gataaacacc cgaccgaaat gctcaacggc attaaaggct taatttacag
    13801 cgattcccgt atccgctacc cgatggtgcg tttagattgg ctcaaaaacc gccataacag
    13861 caacaccgca cagcgtggcg ataaccgctt tgtgcgggta acgtgggacg aagccctcga
    13921 cctgttctac caagagttag aacgggtaca aaaagagtac ggcccgtggg cgttacacac
    13981 cgccaatgtg ggctggcgtt caaccggtca attccacagt tgcggtaacc atatgatccg
    14041 agcgattgcg atgcacggta acagtgtcgg cacggcaggc gactattcta ccggtgcagg
    14101 gcagaccatt ctgccgtatg tgctgggttc aaccgaagtc tattcgcagg gcacgtcttg
    14161 ggaaatcatc ttaaaagaga gcgaaaacat cattttctgg gcaagcgacc cggtgaagaa
    14221 cctgcaagtg ggttggaact gcgaaaccca cgaagcctat gcctacttgg aaaaactgaa
    14281 agagaaagtg gcagcaaaag gcgtgaatgt gatctgtgtt gatccggtga aaagcaaaac
    14341 ccagaatttc ttaggttgcg aacagcagta catcaatcca caaaccgatg tgccgtttat
    14401 gttggcactt gcccacacgc tctacaccga gaacctgtac gacaaaaaat tcttggatat
    14461 gtacacggtc ggctttgaaa aattcctgcc gtatctgtta ggcgaaaccg aagacaaagt
    14521 ggcgaaaacc ccggaatggg cggctgaaat ttgtggtatt tccgccgaca aaatccgtga
    14581 atttgcccga atgcttgccg gcaaacgcac ccaatttatt ttcggctggg caattcaacg
    14641 ccaacaacac ggcgaacaac cgtactggat gggagcggtg ctagcctcaa tgttagggca
    14701 aatcggcttg gaaggcggcg gcatcagcta tgctcaccat tacagctcta tcggtattcc
    14761 tgaatccggt gcggcaatgc cgggggcgtt cccgctcaat attgatgagg ggcaaacccc
    14821 gaaatacgac aacaaagatt acaaaggcta cagcgacacc attccggtgg ctcgtatgac
    14881 cgacagcttg ctccacgccg gcgaaaccat tgattacaac ggcaaaaaag tgacttacgc
    14941 tccgttcaaa atggcgattt tcaccggctg taaccaatgg caccgccatt ccgagcggaa
    15001 caaaatgaaa caggcgttcc aaaaactgga aaccattgtt tcgatcaact acagttggac
    15061 ggcaacctgc cgcttctccg acatcgtgct gccggcctgt accccatttg aacgcaacga
    15121 tattgacgcc tacggctcat acagcaaccg tggcgtgatt gcaatgcaca agctggttga
    15181 tccactctac gactctcgct ccgactttga gattttccga gatctctgcc gccgtttcgg
    15241 taaagagaaa gagtacagcc gtggaatgga cgaaatgcag tgggtagaaa aactctacaa
    15301 agattgtcgc cgtgaaaaca gaggtaaatt tgaaatgccg gagtttgctg aattttggca
    15361 aaaaggctat gtgctgttcc cagagggcaa accttgggtt cgccacgccg atttccgtga
    15421 agatccggaa ttgcacgcac ttggcacacc gtccggcttt atcgaaattt acagcaacaa
    15481 aatcgcctct ttcggctatg acgattgcaa aggtcaccca atgtggtttg agaaagccga
    15541 acgttcacac ggcggtaaaa attccgacaa attcccgtac tggttgcaat cagttcaccc
    15601 ggacaaacgc ttacactctc aactttgtga atcgaaagaa ctgcgtgaaa cttacagcgt
    15661 gcaagggcgt gaaccgcttt acattaaccc acaagatgcc gccaaacacg gcattcaaga
    15721 cggagattta gtgcgagtgt ttaacgaccg tgggcaagcg attgtggggg cggtgctttc
    15781 cgacaacttc ccgaccggcg tagtgcgttt acaagaaggg gcttggtatt caccccttga
    15841 cgaaagcgtg ggggcgattg acacctatgg cgacccgaac acaatgaccc tcgacatcgg
    15901 cacgtccaag ttggcacaag cggtttccgc caacacttgc ttggtgaaca tcgagaaatt
    15961 tatcggcaat gccccggctc cgaatggctt taacggtgcg attgaggtaa cggcttaatg
    16021 acgttggcaa tcagtcgtga agaacggcaa tttatctatg gctggtttaa cgcaatgctc
    16081 gcccgagaac tttcggacga gcagttaaat gccttacaag cgggcgaatt tgacgatttt
    16141 tttgcatacc tcagcgaatt gggttttgcc gaacaggttg caaaatttca agcagaagtg
    16201 accgcttgta ggcagctgga atttcctcgt ctagaactgg cggctgactt tgcccaactg
    16261 tttttattgg aaggcaatct cagtgccatt ccttacgcct ccgcctattt aagcggcgaa
    16321 gcgttagcga aaaatttggc ggaaatggac agactgctgg gcgaatttgg gctgcaagtt
    16381 aatcgagaaa ccaaagagcc gagcgatcac ctttgcgttt atctcaccct ttttgaccgc
    16441 ttgcttgctg aaaaaccgtt ggaaacgcaa cagcaatttc tgcaaaacca actgatgagc
    16501 tggctccctg cgtggttcgc caaagccaaa accgtcaaaa ctaacaccgg cgtttatccg
    16561 gcgttgctaa ggttgtttga acaggtgttg ctgaaggaac aaagataaag ttatttattt
    16621 gagaatgatt tgtattgagc gaattgagaa aggaagttaa aatttcaact tcctttttta
    16681 ttggctggat ttatccacat tattacaagc ggttattttt ataaaaaaat ttacaaagga
    16741 gacattaatg caaaataata aaaccaccta cctaaattgg tacgatttat gcgtgctaac
    16801 cctgatcctg tttggtgagg caatttatac ctcaacccag caatatttca tcaccaccga
    16861 aatgcaaggt aattttgaag aaaatttaac cttttcaaat gaacaaaatt attgggcatt
    16921 actgacgcaa cttgcattgt tgctgattgc aatgggctat ctaaaatggc ggaattttga
    16981 tttcagccgt tgggtgattc gtccctcttg gctggctttt tttagcggta tcgggttgtt
    17041 tatcttgctc tcgttagcga tggatctcta ttttgaaaca ttatatggtg tttacaacct
    17101 agtgtatccg gagccatttt gggcagtatt gagagaagtg aatatttcta ccgtgctgta
    17161 tgcgatttta aacggctttt atgaagaaat ctttttcctc ggtatttgct tggcggttaa
    17221 acctgaatat ttaaaatggg cggtgattta ttcactgatt attcgggtga gttttcatac
    17281 ctatcaagga atggaaagtg cgttaggatt aggaatttgg ctcggtttaa ttatttattt
    17341 gctctactca cgctcaaagc agaaaaacct atttccattt tgggtggcac acagtattgc
    17401 ggatattttg gggctgagtt tgttggctta tttttagtta aaggtgctac atttaagcgg
    17461 cataagttga tagagctgat cgtaccagta gcataaaaac attattattt gtatggaaag
    17521 gtgcttttag gttacaatat aaccgatagt tattggttat aactaaaaat tatttttaat
    17581 ttaacggagc aaatatgaag agtatcaaaa aattaaccct agccactttg attttagtgg
    17641 gtttaactgc ttgtggtagc ggtggttcag gtagcagttc taataatgct aaatcgaaac
    17701 cacaagttga gttaaaaaaa actgaagagg ataaaaaaac tgaagaggat aaaaaaactg
    17761 aagaggataa aaaaactgaa gaggataaaa aaactgaaga ggataaaaaa actgaagagg
    17821 ataaaaaaac tgaagaggat aaaaaggctg aagaagcaaa aaaaattgca gaggcaaaag
    17881 gaattggcaa taaagaatat aaagatggat taaatgagtt agaagaaaaa aatgaaacag
    17941 gtaaaaacgg agaagagatt cgaacccatg gttatctcta taacagccat tattccgttg
    18001 taactgccaa aatgaaacaa actttacagg aaaatggtcg tcaaatggaa cctacggttg
    18061 aagtgaaagg gcttaaaact gagaatttac cgacagaggg caaagcaaca tataaaggag
    18121 aagcctttga ttctcacggt aataacagca attcagtagt aggtggagaa ttaatctata
    18181 atgttgattt ctctagtcgt accggatcag gtctagttaa aaatgtacag ggcggttcta
    18241 tcgagttagc acaaggcgaa ataaaaaatg atagtattat agcttctgct caccaaaagt
    18301 ataatgatca ggctgttgga aatggtagtt acaatatcca attctttggt ccaaatgcag
    18361 aagaaattgg agggaaaatt gagcttaatg gtgagggcga aggctcaatg aagcagatgt
    18421 taggtattgc aggaacccga gaagaacaaa aataacattt aatacccaat gccgtttaat
    18481 cttaaacggc attctttttt actatgtata aaaaacattt tctattttta cttgtttcgc
    18541 ctttagtaat ggcggaaacg gttgagtttc agcaagatcc caaagagttt gatgggcagc
    18601 gacaagcggt taaatttgag caagaaattg caaaaaagga tttaaaatta accgcttact
    18661 tggaggaaga taattctctt aaaggtattg aacagcacat taaccaagcg attcttagac
    18721 aagattgggc gaagctggaa aggctgcttg gcgattatgc aaagcaagag caatttgatc
    18781 cgctactttt agactatgct ttggcggcat tggcttatgc taaaaaagat tatcgctctg
    18841 cggcgtttta ttatcagcag ttgctgaaaa aacagcctga tttaacctac cctcgttttg
    18901 atttggctct gattttaaaa gaggattatc gctacaggga agcagagcaa gaatttaagc
    18961 aactcaaaac cgatgattta cgcaatgcgg tgaacagtgc gttggctcaa atagaggagg
    19021 aagagagctg gaagccggat ttttatttgc aatatactca aaccaacaat gtgaataatg
    19081 cttcttcgtc gccaacggta aatgtaaacg ggcggatttt tattaaagaa tcagacagct
    19141 tgccgcagtc ggcaaaaggc gtaagatatg gcgttggctt gagtaaaatg cgaaatatcg
    19201 gcggtaatca ttttgtggga gtcgatcttg gctataacgg cgtttattac tggaataacc
    19261 acgattatag cgaacagtcg gtctcactaa caccaaaata tagctacaga acggcaactt
    19321 atcgtatcgg ggtttcgccc tttattgagc agaattggct gggtagctca cggtataact
    19381 atcaatttgg tagcagtatc aatgctgtta aactttttga acgagggctg atttccggct
    19441 cattcagcca tctgcaaaaa cgttatagtg aacagcatat tgccgaccgt tacaatggtt
    19501 atcgcaacca actcacgcta ggtacgcaat ggcaagcggt caaaaattgg cgattttttg
    19561 caaatcttaa cgctagccgt gaaattaccc gtgagaaagc tcaatcttcc aataagtggc
    19621 tggcgaccat tggcgtaatt taccaaaccc caagctgggc aagttcggta agtgtcggat
    19681 atggcaagcg ttattttgct gataaacatt atctgtttgg ctataaacgc caagataagg
    19741 aatatcaagc gaatgtggcg gtgtggaatc gaagttggca ctggcaaggg attgtgccga
    19801 aattgaattt ccgttatcaa aaaatcgata gcaatattgc cgatttctac tctcgtagca
    19861 ataaagaagt ctttatgacc ttagagaaac tattttaagg ctgtgtatga atgtcgggga
    19921 gaaaaatttt ctccccgata tttatgtgta gaagatcaga cttgatctga caattcactc
    19981 taaaaagtga gagtttcccg tttagaatat gagtgtcaaa aatcaatcta aacaaaaagg
    20041 aaactctcat gttttattct aacaa
//
LOCUS       DANLUQ010000029        19989 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.029, whole genome shotgun sequence.
ACCESSION   DANLUQ010000029 DANLUQ010000000
VERSION     DANLUQ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 19989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19989
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.029"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            324..620
                     /locus_tag="PXB27_000832"
     CDS             324..620
                     /locus_tag="PXB27_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HDL5345649.1"
                     /translation="MTAITIPQFEEVTSKQFQSEFGRIVDIVKGKTPVAITQYGRPTM
                     VLFPYDQAVEARRLLAKQRLLEMLAERDKNPPAALAEITLEELDKMIDEERNAL"
     gene            610..1026
                     /locus_tag="PXB27_000833"
     CDS             610..1026
                     /locus_tag="PXB27_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HDL5345650.1"
                     /translation="MHSDYLVIDTNILISGLLFPKSNPNLAVKKALLGYTICVSQATF
                     DEFLEVSARPKFAKYFSIQEREEFIANLLDAVKFVEIKETITDCQDPKDNKFLEVAIS
                     AKAKYLVTGDKKDLLSMNPYRNVEIISVSEFLAETR"
     gene            1026..2684
                     /locus_tag="PXB27_000834"
     CDS             1026..2684
                     /locus_tag="PXB27_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HDL5345651.1"
                     /translation="MKRFLTLFLLLFSSFSLAETHQIGILAQRGEAYTRTHWQPWVDW
                     LNSQFPNERFELVPLGINEASRRAELDFLLTNQAQFFYLSNQNVRWLATLNSPFSANG
                     EQGAVGSSIWVRADSHYQQLSDLKNQTISAVDNDAFGGFLLGLYQFHQAGMRQNRDFS
                     VQFSGFPVENALALLAEKQVEAAIVPVCLLEELEKEGKFKRSDFRLILQNPQAQGCLA
                     STPLLPNWSLAAMENVPNELAVQLATRLLNSHNPDLPRWTLPFSSAQADHILRELYRH
                     PQQKSLWATVLDWVRLNKFGLLAVVLFILLNLVALRYQVYRKSKALQQAHCKMQQYQQ
                     ELTRADRLALLGEMTTGFAHELKQPLSAVRMYAEGLKSQNSDPYQQRILDKLIAQVDR
                     AVKTMQSIRDWAQNRPSGEPQAVILNQLITNVIEFVAVENRQNAQISLIADRTFRLNL
                     HATVLEQVLTNCLLNALQAGASEISVRLQAVENGLEIAIEDNGGGFSPAQLEFPFVPF
                     RTDKPHGLGLGLVLCQRLMQSLNGRIVLANGENGAKVVLLLPQI"
     gene            2770..3417
                     /locus_tag="PXB27_000835"
     CDS             2770..3417
                     /locus_tag="PXB27_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL5345652.1"
                     /translation="MIIHILDDEESILDAMSFLLAPLGVEIQTWQSSLDFLAQADLHR
                     QGVLLLDIRMPLPDGQQVHQQLREAQSTLAVVIMTAHGDVPMAVAELKKGAVDFLQKP
                     ASFEQLKQAIAQAEKVSEKAVKIREISQNYAKLTEKERNLVPLIMQGFTNKQIADKLA
                     ISVRTVEVHRANVMEKMQAESLAGLVQMIGELQYKGSGNCSATFSFDFCRINLLL"
     gene            complement(3401..4339)
                     /gene="ttcA"
                     /locus_tag="PXB27_000836"
     CDS             complement(3401..4339)
                     /gene="ttcA"
                     /locus_tag="PXB27_000836"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HDL5345653.1"
                     /translation="MTDDIQKSDKKITYNFNKLQKRLRRNVGNAIADFGMIEEGDRVM
                     VCLSGGKDSYTLLDILLNLQQSAPVNFSVVAVNLDQKQPGFPEEVLPRYLESIGVEYK
                     IVEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDMLATLF
                     LNMFYGGKMKSMPPKLISDDGKQIVIRPLAYCKEKDIIKYAEAKQFPIIPCNLCGSQP
                     NLQRQVVKEMLNTWDRQYPGRLETMFSALQDIVPSHLCDPKLFDFKGIKHGQTIEGIE
                     GDTAFDDVKIEAKTFLYEDDDNNFAEAGMIQFKEVN"
     gene            complement(4402..5115)
                     /gene="ubiG"
                     /locus_tag="PXB27_000837"
     CDS             complement(4402..5115)
                     /gene="ubiG"
                     /locus_tag="PXB27_000837"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HDL5345654.1"
                     /translation="MQNVDQQEITKFENMAKTWWDPNGSFKPIHLLNPLRLAYILEKS
                     NGLFGKKVLDVGCGGGILSEAMAKQGAIVTGIDMTSQPLEVAKQHAKESGLEIDYQQT
                     TIENFLAKMTACNQEKFDVITCMEMLEHVPDPLSIIQSCKALLKPDGVLFFSTINRTF
                     KAYMLVIIGAEYVLKMLPKGTHEFEKFIKPAELLAWCDQADLRCQEMRGYHFNPLTEK
                     FWLNNDVSCNYIATLKPNN"
     gene            5180..5560
                     /locus_tag="PXB27_000838"
     CDS             5180..5560
                     /locus_tag="PXB27_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HDL5345655.1"
                     /translation="MTRINLVPPTELCDQHLLAEHRELTRIPNAVAKGKFSLKGQPED
                     YKLGEGHVRFFFNKLTFLKKRYDALHEECLARGFKVQYFWAEHLPQDPSLWLDYQPTE
                     TALTVNRERIALRMPANPRFSERK"
     gene            5657..8353
                     /gene="gyrA"
                     /locus_tag="PXB27_000839"
     CDS             5657..8353
                     /gene="gyrA"
                     /locus_tag="PXB27_000839"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HDL5345656.1"
                     /translation="MSELAKDIIPVSIEDELKTSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLFSMDQNSNTYNKPHVKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDAPAAMRYTEVRMQKITQELLTDLDKETVDFSPNYDGKEMIPDVLP
                     TKIPALLVNGSSGIAVGMATNIPPHNLGEVLDGCLAYIENEQISVEELMRYIPGPDFP
                     TGAIINGRKGIEEAYRTGRGKVYVRAKASVETTDKGREQIVVTELPYQVNKAKLIEKI
                     AELVKEKKIEGISKIDDYSDKKGISIVIEVKRDAVGEVVLNHLYSLTQMQVTFGINMV
                     ALDNGQPKVLNLRQIIEAFVKHRREVVTRRTIFELRKARERAHILEGLAIALANIDPV
                     IELIRASKTAEEAREGLLARPWALGNVAPMLEAAGVGASRPEDLPEHLGVRDGEYYLS
                     EAQARAILELRLHRLTGLEHEKIVEEYKELLTEIGELIHILTSPERLMEVIREELERV
                     KTDFNDERRTEITAASGDINIEDLIAQEDVVVTLSHAGYVKYQPLSDYEAQRRGGKGK
                     SATKMKEDDFIEKLLVANTHDTILCFSSRGRLYQLKVYQLPQASRGARGTPIVNILPL
                     VKEENERITAILPIPNGEFSADKFIFMATASGVVKKVSLDAFSNVRASGLIALKLREG
                     DELIGVDITDGNSEIMLFSAQGRVVRFAETAVRAMGRTATGVRGIKLSTTEISSEEIE
                     EAEIIETDNEEAEDSSSVSLDKDRVVSLVIPRTEGDILTVTQNGYGKRTVISEYPVKS
                     RATKGVVSIKVNERNGKVVAAVQVEETDQIMLITDAGTLVRTRVAEVSLVGRNTQGVR
                     IIRTAEDEQVVSLERVCEPEDDENESLDALENAETAETSTEE"
     gene            8448..10412
                     /locus_tag="PXB27_000840"
     CDS             8448..10412
                     /locus_tag="PXB27_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5345657.1"
                     /translation="MALINLSNAYLGFGDHPLLDHTELHIEPGERVCLVGRNGAGKST
                     LMKILAGEVQLDDGKLVFEKDIVVTRLEQDPPRHIEETVFDYVAEGIAHLADLLKQYH
                     HISLQMQTDYSDELLAKLSAVQSQLEHANGWQFENRIQDTLKLLELAPDKRLCELSGG
                     WVRRAALARALVADPDVLLLDEPTNHLDIEAIAWLENLLLDFKGSIIFISHDRAFIRK
                     MATRIVDLDRGKLVSYPSNYDKYLEEKAEDLRVEALQNELFDKKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKALREERRNRREVQGTAKIQIDNTARSGKIVFEVENAGYEIGGRTL
                     LKNFSTTILRGDKIALIGANGIGKTTLIKLLLGEIQPTSGSVRCGTKLEVAYFDQYRV
                     DLDPEKTVMDNVADGKQDVEVNGVKRHVLGYLQDFLFPPKRAMTPVKALSGGERNCLL
                     LAKLLLKPNNLLILDEPTNDLDVETLELLEEMLTDYQGTLIIVSHDRQFVDNTVTECY
                     FFEGEGVVNKYVGGYFDAKQQQENYFAATADKRLNSSKNLQKIEEKAPLAEQEKQKAD
                     QEKTKAEPTKKTKLSYKEQRELDELPAKMEQLEADIEALQAEVNSAEFFAKDPSYTNA
                     QLQKLADTEAALEQAFERWEALENIKNGNI"
     gene            10494..11252
                     /gene="tcdA"
                     /locus_tag="PXB27_000841"
     CDS             10494..11252
                     /gene="tcdA"
                     /locus_tag="PXB27_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HDL5345658.1"
                     /translation="MTERLDNYEQRFGGIGRLYTPEGLEKLRRSHICVIGIGGVGSWA
                     VEALARSGIGKITMIDMDDICVTNINRQIHAMSGTVAQLKTEAMKERIERINPECVVE
                     IIDDFLTQDNLADYLNRGYDYVIDAIDSLRVKAAMIAFCKRNKIKIITTGGAGGQTDP
                     TKIQITDLSKTVQDPLAARVRSLLRKEYGFSNKNKFGVECVSSTQQLIFPQMGEACEV
                     SATMNCANGFGAVTMVTATFGFFAVSRVIDKLLK"
     gene            11299..12666
                     /locus_tag="PXB27_000842"
     CDS             11299..12666
                     /locus_tag="PXB27_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HDL5345659.1"
                     /translation="MAIHRVNPKGSMEQLSHLEMELLAKNTQGNLHQLYRNCSLAVLN
                     SGVHTDDSRALLSQYPDFEIRLLTREKGVSLELHNPPETAFVDGKMILNIQYHLFAVL
                     RDIVFVNALKNAIRPLEETSLEALTTNTVFSILRNAKAIEMNTDPNLVVCWGGHSINE
                     TEYQYCRAVGQEFGLRELNIVTGCGAGVMEAPMKGAAIGHANQRYKNGRFIGITEPSI
                     IASEPPNPIVNELVIMPDIEKRLEAFVRLGHAIVVFPGGPGTLEELLYIIGIKLNPAN
                     HAQQLPLILTAPKESADYFYAIDQFIGETLGEEARSLYEIIIDDSVLVARKVKQAMIE
                     VKSSRYESGDAYNFNWSLKIEEDFITPFIPTHENMANLNLSLAQPKEQLAANLRRAFS
                     GIVAGNIKPETSDLIEKYGVFKLHGDRKLMEHIDKLLERFVVQHRMKIPTGEAYQPCY
                     EIVKG"
     gene            12686..14233
                     /locus_tag="PXB27_000843"
     CDS             12686..14233
                     /locus_tag="PXB27_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HDL5345660.1"
                     /translation="MYLKHIEISGFRGINHLSLQLRPNMVLIGENAWGKSSLIDALSL
                     IFNLEQALYQFQFKDFHIQYTDTPNQVRDIQLVFTFCEEHQHDLAVQFPAYLAQAAFY
                     IDNHRCVSLAVTGKLHGEQISTDYQFVDQSLKPIQIEQAENAIRQFIYHHPVYRLRDA
                     RLNNRVHNELLLKGSSQLEDDFQRELNALSSLLKYYFLTSQSRYLIKTSMQDTALWWE
                     KVKSLCLRLKQQPELVESAQQHISTLFLFNPRSTVIEKPIILFEDLNAQLHPRMIAIF
                     WELLGFLPSQRITTTNSIELLSQVPLREICRLVRHHDHTQAYWLERHSLGKEDLRKLT
                     FHIHHNRGLALFAKAWILVEGETEVWILQELARLLDIHLEMEGIKIVEFAQCGLRPLI
                     KYARAMGIEWYVLTDGDDSGKRYADTVKSMEEDFAHRLTMLPQKDIEHFFYKAGLRNV
                     FIQLANWRSQEKYPVSYIIKRAIQRSSKPDLAIALANEMGQRGEHSIPTLFKQLFADV
                     LALINKV"
     gene            14456..15490
                     /gene="metN"
                     /locus_tag="PXB27_000844"
     CDS             14456..15490
                     /gene="metN"
                     /locus_tag="PXB27_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HDL5345661.1"
                     /translation="MIQLKNISKQFEVKGKKITALDNVNLEVPRGTIYGVIGASGAGK
                     STLIRCVNLLERPTIGQVIVDGQDLTAMSDKELIHARRNIAMIFQHFNLLSSQTVFEN
                     VALSLRLSNTPKEQVEKKVNELLDLVGLTDRKDVYPANLSGGQKQRVAIARALASDPH
                     VLLCDEATSALDPATTQSILQLLKDINKRLGLTILLITHEMEVVKRICDRVAVIDKGQ
                     LIESGSVSEIFSNPKTELAQKFIQSTFHFELPQEYTEQLSSSPTEHSKPIIKFEFTGR
                     SVDAPLLSMASKKFGIDFSILMSQIDYAGGVKFGFVIAEVSGNPDSIAEAKFYLIDNN
                     VKVEVLGYVG"
     gene            15480..16157
                     /locus_tag="PXB27_000845"
     CDS             15480..16157
                     /locus_tag="PXB27_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5345662.1"
                     /translation="MWADFMQEMTPQMWSLVWESTFETVYMGFVATALAVVIGLPIGF
                     LAFLTGKGEILENPRLHQVLDVVINIGRSIPFIILLIILLPFTRLVVGTTLGTTAAIV
                     PLSVSAIPFFARLTSNALLEIPSGLTEAAKSMGATNWQVVTKFYLPESLPILINGITL
                     TLVALIGYSAMAGAVGGGGLGNLAISYGEHRNMVYVKWIATIIIVAIVMISQKLGDHL
                     AKRYDHR"
     gene            16180..17013
                     /locus_tag="PXB27_000846"
     CDS             16180..17013
                     /locus_tag="PXB27_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family lipoprotein"
                     /protein_id="HDL5345663.1"
                     /translation="MSFKKILGVALVSALALTACKEEKKAESTAAPAAQAPAKIKVGV
                     MSGPEHTVAERAAQIAKEKYGLEVEFVLFNDYALPNTAVSKGDLDANAFQHKPYLDKD
                     SQSKGLNNLVIVGNTFVYPLAGYSKKVKNVSELAEGAVIAVPNDPSNLARALILLEKQ
                     GLIKLKDNTNLFSTSVDIIENPKNLKIKEVDTSIAAKALDDVDLAVVNNTYAGQVGLN
                     TQDHGVFVESKDSPYVNIIVARQDNKDAANVQNFIKSYQTEEVYQEAQKHFKDGVVKG
                     W"
     gene            17096..17926
                     /locus_tag="PXB27_000847"
     CDS             17096..17926
                     /locus_tag="PXB27_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family lipoprotein"
                     /protein_id="HDL5345664.1"
                     /translation="MNFKKLLGVALVSALALTACKDEKAQAPATTAKTENKAPLKVGV
                     MTGPEAQMTEVAVKIAKEKYGLDVELVQFTEYTQPNAALHSKDLDANAFQTVPYLEQE
                     VKDRGYKLAIIGNTLVWPIAAYSKKIKNISELKDGATVAIPNNASNTARALLLLQAHG
                     LLKLKDPKNVFATENDIIENPKNIKIVQADTSLLTRMLDDVELAVINNTYAGQAGLSP
                     DKDGIIVESKDSPYVNLVVSREDNKDDPRLQTFVKSFQTEEVFQEALKLFNGGVVKGW
                     "
     gene            17978..18769
                     /gene="metQ"
                     /locus_tag="PXB27_000848"
     CDS             17978..18769
                     /gene="metQ"
                     /locus_tag="PXB27_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HDL5345665.1"
                     /translation="MKIMKLAGAVAIFSLFLTACNDKAEKLKVGVISGPEHKVMEVAA
                     KIAKEKYNRDVELVVFTDYATPNAALDKGDLDLNAFQHKPYLDNQIQEKGYKLVPVGN
                     TFVYPIAAYSKKIKSLAELKDGDTIAVPNDPTNLARALILLEKQDLIKLRADAGLKAT
                     SVDIIENPRKLVIQEIEAPLLPRTLDDVAFSIINTTYAGQNGLTPTKDGIFVEDKDSP
                     YVNLIVARENNQHSEAVKDLVKAYQTEEVYNKANEEFKGAMIKGW"
     gene            complement(18814..19965)
                     /gene="metK"
                     /locus_tag="PXB27_000849"
     CDS             complement(18814..19965)
                     /gene="metK"
                     /locus_tag="PXB27_000849"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018356717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDL5345666.1"
                     /translation="MAINLFTSESVSEGHPDKIADQISDAVLDEILKQDPKARVACET
                     YVKTGMALVGGEITTSAWVDIENLTRQVICDIGYTHSDMGFDAHSCAVLNAIGKQSPD
                     INQGVDRADPLEQGAGDQGIMFGYATNETEVLMPAPITYAHRLMEQQSKVRKSGKLDW
                     LRPDSKSQLTFAYENNKIIGIDAVVLSTQHAEHISQKELIEGVMEEIIKPVLPSEWLS
                     QNTKYFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQLSYAIGVADPTSIMVETFGTGKVSNEILVKLIYQNF
                     DLRPYGLIKMLDLIKPIYRETAAYGHFGRKHFPWEKTDKAEALRAGAGL"
BASE COUNT         5884 a         3998 c         4691 g         5416 t
ORIGIN      
        1 aaatgcctct gtgaaaatca cagccatact aggaaataaa aggatttaat ttataaatga
       61 attgactata agcaatataa tctatcgaat tatctctttt gttcaaggca agtatggata
      121 cctgaccgca ggcctggaga tagaacctac atataatatg ctctactaca ttaagccata
      181 aaaaatagaa aatcaagtgt ttgttcaagt ttttatgtta ttgaatttac aataactaca
      241 aaaatttatc ttaattggct cgattgattt ttacacaaaa catagctaac atatccaaaa
      301 tcactcacat taggagctta aaaatgacag cgattactat ccctcaattt gaagaggtga
      361 cttctaagca atttcaatct gagttcggca gaattgttga tatcgtaaaa ggcaaaaccc
      421 ctgtggcgat tacccaatat ggcagaccga caatggtgct ttttccttac gatcaagcgg
      481 ttgaagctcg gcgattatta gcaaaacagc gtttgcttga aatgcttgca gagcgagata
      541 agaatccgcc ggcagctctt gcagaaatca cgcttgaaga gttggataaa atgattgatg
      601 aggaacgtaa tgcactctga ttatttagtg attgatacga atattttgat tagtggttta
      661 ttgttcccaa aatcaaatcc caatttagca gtgaaaaaag ccttattagg ctataccatt
      721 tgtgtctcgc aagcgacttt tgacgaattt ttagaagtct ccgctagacc gaaatttgct
      781 aaatatttca gtattcagga aagggaagaa tttattgcca atttgttgga tgcagtaaag
      841 tttgtggaaa ttaaagaaac gattacagat tgccaagatc ccaaagataa taaattttta
      901 gaagtggcga tcagtgcaaa agcgaagtat ttagtgacgg gcgataaaaa agatctcctc
      961 agtatgaacc cttatcggaa tgttgaaatt atttccgtca gcgaattttt agcggaaact
     1021 cgctaatgaa acggtttctc actctttttc ttctcctttt ttcatctttt tcattagccg
     1081 aaacccacca aatcggcatt ctcgctcagc gtggggaagc ctacacccgc acccattggc
     1141 agccttgggt ggattggtta aattcgcagt ttcccaacga gcggtttgag ctggttccat
     1201 tggggattaa cgaggcgagc cgccgggcgg agttggattt tctgctgacc aatcaagctc
     1261 agtttttcta tttgagtaac caaaatgtgc gatggcttgc cacgctcaac agtccgtttt
     1321 cggcaaatgg tgagcaaggg gcggtgggca gtagcatttg ggtgcgtgcc gacagccatt
     1381 atcagcagtt aagcgatctg aaaaatcaga ctatcagtgc ggtggataac gatgctttcg
     1441 gcggtttttt gcttgggttg tatcagtttc atcaagcagg aatgcggcaa aatcgggatt
     1501 tttcggtgca gtttagcggt tttccggtgg aaaatgcgtt ggctttgctg gcggaaaaac
     1561 aggtggaagc ggcgattgta ccggtctgtt tgctggaaga gttggagaaa gagggcaagt
     1621 tcaagcggtc ggattttcgc ttaattttgc aaaacccaca ggcgcagggt tgccttgcca
     1681 gcacgccgtt gttgccgaac tggtcgctgg cggcaatgga aaatgtgcca aacgagctgg
     1741 cggtacagct tgccacacgc ttgctaaaca gccacaatcc cgacctaccc cgctggacgt
     1801 tgccgttttc ttccgcccaa gccgatcaca ttctgcgtga actgtatcgc cacccgcaac
     1861 aaaaaagcct gtgggcaacg gtgctggatt gggttcgtct caataaattc ggcttgttgg
     1921 cggtggtgtt gttcattttg ctcaaccttg tcgccctgcg ttatcaggtc tatcgcaaaa
     1981 gcaaggcgtt acagcaggca cactgcaaaa tgcagcagta tcagcaggaa ctgacccgag
     2041 ccgaccgcct tgctttgttg ggcgagatga caaccggttt tgctcacgaa ctgaaacagc
     2101 cgttgtcggc ggtgcgaatg tacgccgagg ggctgaaaag ccaaaacagc gatccttatc
     2161 aacagcggat tttggataag ctgattgcgc aggtagatcg ggcagtgaaa acgatgcaga
     2221 gcattcggga ctgggcgcaa aaccgcccga gtggtgaacc gcaagcggtc attttgaacc
     2281 aattgattac taatgtgatc gagtttgtgg cggtggaaaa tcgccagaat gcacaaatct
     2341 cgctgattgc cgaccgcact tttcggctta atttacacgc cacggtactg gaacaagtac
     2401 tgacgaactg cctgctcaac gccctgcaag ccggtgcaag cgagatatcg gtgcggttac
     2461 aagcggtcga aaacgggctg gaaattgcaa tcgaagacaa cggcggcggt ttttcccctg
     2521 cccagcttga atttccgttt gtgccatttc gcaccgacaa accacacggc ttagggctag
     2581 gcttggtgct atgccaacgg ctgatgcaat cgctcaacgg acggattgta ttggcgaacg
     2641 gggagaacgg ggcgaaggta gtgcttttac tccctcaaat ataaatgtag gggcaggtcc
     2701 tgtgcctgcc cgtaaatcct atcaaattca gggcgggcac aggacccgcc cctacggaaa
     2761 aaagaaaaaa tgattattca cattcttgat gacgaagaaa gcattttaga cgctatgtcg
     2821 ttcctgcttg ccccgcttgg ggtggaaatc caaacgtggc aatccagcct agattttttg
     2881 gcacaggcgg atttacaccg gcaaggcgtg ctgttactag atattcgaat gccgctgcca
     2941 gacgggcaac aggttcatca acaactgcga gaggcacaat cgaccctggc ggtggtgatt
     3001 atgaccgccc acggtgatgt gccgatggca gtcgctgaat tgaaaaaagg cgcagtggat
     3061 tttctgcaaa agccggcaag ttttgaacag ctaaaacagg cgattgcaca ggcggaaaaa
     3121 gtaagtgaaa aagcggtgaa aattcgtgaa atttcgcaaa attacgccaa attgacggaa
     3181 aaagagcgta accttgtgcc actgattatg caaggtttca ccaataaaca gatcgcagat
     3241 aaattagcga tttcagtgcg tacggtggaa gtacatcgag ccaatgtgat ggaaaaaatg
     3301 caggcggaga gtttggcggg gctggtgcag atgatcgggg agttacaata taaaggtagc
     3361 ggaaattgct ctgctacctt tagtttcgac ttttgccgta ttaatttact tctttaaact
     3421 gaatcattcc cgcttcggca aaattgttat cgtcatcttc gtataagaag gttttggctt
     3481 caattttgac atcatcaaaa gcggtgtcgc cttctatgcc ttcaatggtt tgaccgtgtt
     3541 taatcccttt aaaatcaaac aatttcggat cgcataagtg ggatggcacg atatcttgca
     3601 aagcactgaa cattgtttct aaacgccccg ggtattggcg atcccaagta ttcagcattt
     3661 ctttcaccac ttggcgttgt aaattaggct gagaaccaca cagattacac ggaataatcg
     3721 ggaactgctt ggcttcggca tatttgataa tgtctttttc tttacaatag gcgagtgggc
     3781 ggatcacaat ttgcttgccg tcatcagaga tcagtttagg tggcatactt ttcattttgc
     3841 cgccgtagaa catatttaag aataaagtcg ccagcatatc gtcacggtga tgccctaatg
     3901 caattttggt tgcacccaat tcggtggcgg tgcggtataa aatcccacgg cgaagtcgtg
     3961 agcaaagtga acaggtggtt ttgccctccg gaattttctc ttttacgatg ccgtaggtgt
     4021 tttcttccac aattttgtat tccacgccaa tgctttctaa atagcgaggc aatacctctt
     4081 ccggaaatcc cggttgcttt tggtctaaat tgaccgctac cacactaaaa ttcaccggtg
     4141 cactttgctg taaattgagc aaaatatcta acagcgtata gctgtcttta ccgccggaaa
     4201 ggcagaccat tacacggtcg ccctcttcaa tcataccaaa atcggcaatg gcattaccga
     4261 cattacggcg taaacgtttt tgtaacttgt tgaaattata agtaattttt ttatcagatt
     4321 tttgaatatc gtctgtcata atagttgtac taggcttaga aattagcggt gtatagtaaa
     4381 gatttcgcac tattttgcaa gttaattatt cggcttcagc gtagcgatat agttacaact
     4441 cacatcatta ttgagccaaa atttttcggt taatgggttg aaatggtagc ctctcatctc
     4501 ttggcaacgt aaatcagcct gatcgcacca agctaacaat tcggcaggct tgatgaattt
     4561 ttcaaattcg tgcgtgcctt ttggcaacat tttcagcaca tattccgccc cgataattac
     4621 caacatatag gctttaaaag tacggttgat ggtagagaaa aacagcacgc cgtcaggttt
     4681 gagcagggct ttacagcttt gaatgataga aagcggatcg ggaacgtgtt ccagcatttc
     4741 catacaagta atcacatcga atttttcttg attacaagcg gtcatttttg ctaaaaaatt
     4801 ttcaatcgtg gtttgttggt aatcaatctc taacccactt tctttggcgt gctgctttgc
     4861 cacttcaagc ggttgagagg tcatatcgat gccggtaaca atcgctccct gttttgccat
     4921 tgcctcactt aaaataccgc caccacagcc gacatctaac acctttttgc caaataaacc
     4981 gttcgatttt tctaaaatat aggctaaacg cagtggatta agcagatgaa tcggcttgaa
     5041 actgccgttt ggatcccacc aggttttagc catattttca aattttgtga tttcttgctg
     5101 atcgacgttt tgcataaact gttcctatgg taaatttagg gcattactat aacgcattta
     5161 gcctaaaagg aaaatcagaa tgacccgaat taaccttgtt ccgccaacgg aattatgcga
     5221 tcaacatctg cttgccgaac atcgtgaact tactcggatt ccgaatgcgg tagcgaaagg
     5281 caaatttagc ctaaaaggac agccagaaga ttacaaactg ggcgagggac acgttcggtt
     5341 tttcttcaat aaactcacgt ttttgaaaaa gcgttacgat gcgttacacg aggaatgttt
     5401 agcaagaggt tttaaggtgc aatatttctg ggcggaacat ttaccacaag atccctctct
     5461 ctggctagat tatcagccga ctgaaaccgc tctgactgta aatcgggagc gaattgcatt
     5521 aagaatgccg gcaaatccac gttttagcga aagaaaatga ccgcttgtac gcctttttgt
     5581 tgctttttat gctataatct cgccaatttt ttcgccttat ttaaggctct tttactaata
     5641 aaattaggaa atttcaatga gcgaattagc caaagatatt attcccgtaa gtattgagga
     5701 cgaattaaaa acctcttacc ttgactatgc gatgtcggtg attgttgggc gtgccttgcc
     5761 tgatgtacga gacggtttaa agccggtaca ccgccgagtg cttttttcaa tggatcaaaa
     5821 cagcaacacc tataataagc cgcacgttaa atctgcccgt gtggtcggtg atgtgatcgg
     5881 taaatatcac ccacacggtg actctgcggt gtatgacact attgtgcgta tggctcaacc
     5941 gttctcacta cgttatatgt tggtggacgg gcaaggtaac tttggttcta ttgatggcga
     6001 tgcaccggca gcaatgcgtt ataccgaagt gcgtatgcag aaaattacgc aggaattatt
     6061 aaccgatctc gataaagaaa cggtggattt ctcaccgaac tatgacggca aagagatgat
     6121 tcctgatgta ttgccaacca aaattccggc tctgttagtc aatggttctt cagggattgc
     6181 ggtcggtatg gcaaccaata ttccacctca taatttaggc gaagtattag atggttgctt
     6241 ggcgtatatt gaaaatgaac agattagtgt tgaagagcta atgcgttata tcccggggcc
     6301 ggatttcccg accggggcga ttatcaacgg ccgtaaaggg attgaagagg cttaccgcac
     6361 cggtcgtggc aaggtgtatg tgcgcgcgaa agccagcgtg gaaacaactg ataaagggcg
     6421 tgagcaaatt gttgtaaccg aactgccata ccaagtgaat aaggcaaaat taattgaaaa
     6481 aattgccgag ctggttaaag agaagaaaat cgaaggcatc agcaaaattg atgactattc
     6541 cgataaaaaa gggatttcga ttgttattga agtcaagcgt gatgcagtgg gtgaagtggt
     6601 gttaaaccac ctttattctt taacccaaat gcaggtcacg tttggtatca atatggtggc
     6661 gttagataat gggcagccaa aagtgctgaa cttacgccag atcatcgaag cctttgttaa
     6721 acaccgtcgt gaagtggtca cacgccgtac gatttttgaa ttgcgtaaag cccgtgaacg
     6781 tgcccatatt ttagaaggtt tggcgattgc gttagcgaat atcgacccgg taattgaatt
     6841 aattcgagcc tcaaaaaccg cagaagaagc ccgtgaaggc ttattggctc gcccttgggc
     6901 attaggcaat gttgcaccaa tgttagaggc tgctggtgtt ggtgcttccc gaccggaaga
     6961 tttacctgaa cacttaggag tgcgtgatgg cgaatactat ttatctgaag ctcaagccag
     7021 agcgatttta gagctacgct tacaccgctt aaccggttta gaacacgaga aaatcgttga
     7081 agaatacaaa gaactcttaa ctgaaattgg cgaattaatc cacattttaa ccagcccaga
     7141 acgcttaatg gaagtgatcc gtgaagagtt agaacgtgta aaaaccgatt ttaatgatga
     7201 acgccgtact gaaattaccg cagcctctgg cgacatcaac attgaagact taatcgcaca
     7261 agaagatgtg gtggttacgc tttctcacgc cggctatgtg aaatatcagc cgttatccga
     7321 ctacgaagca caacgccgtg gcggtaaagg taagtcggca accaagatga aagaagatga
     7381 tttcatcgaa aaactcttgg tggcaaacac ccacgacacc attctttgct tctcaagccg
     7441 tgggcgttta tatcaattaa aagtgtatca attaccgcaa gcaagccgtg gggcaagagg
     7501 cacgccgatt gtgaatattc tgccgttagt gaaagaagaa aacgaacgta ttaccgcgat
     7561 tctaccgatt ccaaacggtg aatttagtgc cgataagttt atctttatgg caacagctag
     7621 cggtgtagtg aaaaaagttt cccttgatgc ctttagcaac gtgcgtgcaa gcggtctgat
     7681 tgcgttaaaa cttcgtgaag gcgatgagct gatcggggta gatattactg atggtaacag
     7741 cgaaattatg ttgttctcgg cacaagggcg agtagttcgc tttgccgaaa cagcagttcg
     7801 tgcgatggga cgtaccgcca ccggcgtacg aggtattaaa ctttctacca ccgaaatttc
     7861 aagcgaagaa attgaagaag ctgaaattat tgaaactgac aatgaagagg cagaagattc
     7921 ctccagcgtc agccttgata aagaccgtgt ggtttcttta gtgattcctc gcaccgaagg
     7981 cgatatttta accgtcaccc aaaacggcta cggtaaacgt acggtgatta gcgaatatcc
     8041 ggtgaaatcc cgagcaacca aaggggtcgt ttcgattaaa gtgaatgaac gaaacggcaa
     8101 agtggttgcg gcagttcaag tagaggaaac cgaccaaatt atgttgatta ccgatgccgg
     8161 cacgctggtt cgcacccgag tggcagaagt cagcctcgtt ggacgaaaca cccaaggcgt
     8221 acgtattatt cgcaccgcag aagacgaaca agttgttagc cttgaacgag tctgcgagcc
     8281 tgaagacgat gaaaatgaaa gcctagacgc attagaaaat gccgaaacag cagaaacatc
     8341 aactgaagaa taatcttaac taaacaagcg gtcgaatttt cacaattttt tgcaaaaata
     8401 gaaggaaatt cgaccgtttt tggatcatac agtagataat aaaaactatg gcacttatca
     8461 atctttccaa cgcctattta ggctttggcg atcatccttt attggatcat actgaattac
     8521 atatcgaacc cggcgagcgg gtttgtttgg tggggcgtaa cggggcgggt aaatcgaccc
     8581 tgatgaaaat tctggcgggc gaagttcagc ttgatgacgg caagttggta tttgaaaaag
     8641 acattgtcgt tacacgtctt gaacaagatc cgccccgcca tattgaagaa acggtgttcg
     8701 attatgtggc ggaggggatt gcccatcttg ccgacttatt gaagcaatat caccacattt
     8761 ccctgcaaat gcagaccgat tacagcgatg aactgctcgc caaactttca gcggtgcaat
     8821 cgcaattaga acacgctaac ggttggcagt ttgaaaaccg cattcaagac accttgaaac
     8881 tgctggaact cgcccccgac aaacgcctgt gcgaactctc aggcggttgg gtacgccgtg
     8941 cggcattggc tcgggcgttg gttgccgatc ctgatgtatt gctgttggac gaaccgacca
     9001 accacctcga cattgaggcg attgcgtggc tggaaaatct gttattggat ttcaaaggca
     9061 gtattatttt tatctcgcac gaccgtgcct ttatccgcaa aatggcgact cggattgtgg
     9121 atttagatcg aggcaaattg gtttcctacc caagcaatta cgataaatac ttggaagaaa
     9181 aagccgagga tctgcgagta gaagcgttgc aaaatgagct gttcgacaaa aaactcgccc
     9241 aagaagaagt ctggatccgt caagggatta aggcaagacg cacccgaaac gaagggcgtg
     9301 tgcgagcgtt gaaagcgttg cgtgaagagc gtagaaaccg ccgagaggta caaggcacgg
     9361 cgaaaattca gatcgacaac accgcacgca gcggcaaaat tgtgtttgaa gtggaaaatg
     9421 ccggctatga aatcggcggc agaaccctgc tgaaaaattt cagcaccacc attttgcgtg
     9481 gcgataaaat tgccctgatt ggagcgaacg ggatcggcaa aaccacgctg atcaagctgt
     9541 tacttggcga aattcagccg acatctggtt cagtgcgttg tggcacaaaa ttggaagtgg
     9601 cgtacttcga ccaataccga gtcgatcttg accctgaaaa aaccgtgatg gacaacgtgg
     9661 cagacggcaa gcaagatgta gaagtaaacg gggtgaaacg ccacgttctt ggctatctgc
     9721 aagacttcct gttcccgcca aaacgggcga tgacaccggt gaaagccctc tcgggtggtg
     9781 aacgcaactg cttgttgctg gcaaaactgt tactcaaacc gaacaacttg ctgattttgg
     9841 acgaaccgac caacgatttg gacgtggaaa ccctcgaact gctggaagag atgctcaccg
     9901 actatcaagg caccctgatt attgtgagcc acgaccgtca atttgtggat aataccgtaa
     9961 ccgaatgcta cttcttcgag ggcgaaggcg tggtgaataa atatgtcggc ggctatttcg
    10021 atgccaaaca acagcaggaa aattacttcg ccgccactgc cgacaagcgg ttaaattcga
    10081 gtaaaaattt gcaaaaaata gaggaaaaag caccgcttgc ggagcaagag aaacagaaag
    10141 ccgatcagga aaaaacgaaa gcagagccga cgaagaaaac caaactttcc tacaaagaac
    10201 agcgtgaact ggacgagtta ccggctaaaa tggaacagct tgaagccgac attgaagccc
    10261 tgcaagccga agtaaatagt gccgaattct tcgccaaaga tccaagctac accaacgccc
    10321 agctacaaaa acttgccgat accgaagccg cgttagagca agcctttgag cggtgggaag
    10381 cgttggaaaa tattaaaaac gggaatatct aatgtagggg cgaatgatta ttcgcccttg
    10441 atggataaaa tgattgggcg aaaaattttt cgcccctaca aatgtgaagc aaaatgacag
    10501 aaagattaga taactacgaa caacgttttg gtggcattgg tcgcctttat acgccggaag
    10561 gattggagaa actacgccgt tcgcatattt gcgtgattgg gattggcggg gttggctcgt
    10621 gggcagtgga ggcattggct cgctcaggta tcggtaagat cacgatgatc gatatggacg
    10681 atatttgcgt gaccaatatc aaccgccaaa ttcacgcaat gagtggcacg gtggctcaac
    10741 tgaaaaccga agcgatgaaa gagcggattg agcggatcaa ccctgaatgt gtggtggaaa
    10801 ttattgatga tttcttaacg caggataatc tcgcagacta cctcaatcga ggatatgact
    10861 atgtgatcga tgcgattgac agtttgcggg taaaagcggc gatgattgcg ttttgtaaac
    10921 gcaataagat caaaattatc accaccggcg gggcgggcgg gcagaccgat ccgaccaaaa
    10981 ttcaaattac cgatttaagc aaaacggtgc aagatccgtt ggcggcaagg gttcgtagcc
    11041 tgttgcgtaa agagtacggt ttcagcaata aaaacaaatt cggcgtggag tgcgtttctt
    11101 cgactcagca actgattttc ccacaaatgg gtgaggcctg tgaggtgtcg gcaacaatga
    11161 actgtgccaa cggctttgga gcggtgacaa tggtgacggc aacgttcggc ttttttgcgg
    11221 tgagtcgggt gattgataaa ctattaaaat aacaagcggt catattaaat gaattttttg
    11281 caaatcaagg agtaaataat ggcaatacat cgagtcaacc cgaaagggag tatggaacag
    11341 ctttcccatt tggaaatgga gttgttggca aagaatacgc agggtaattt gcatcaactt
    11401 tatcgtaatt gctctttggc ggtgctaaat tccggtgtgc ataccgatga cagccgagcg
    11461 ttgttaagcc aataccctga ttttgaaatc cgcttattaa cccgtgaaaa aggcgtgtcg
    11521 cttgaattgc ataatccacc cgaaactgcg tttgttgacg gcaagatgat tttgaatatt
    11581 caatatcacc tttttgcggt gctgcgggat attgtgtttg tgaatgcgtt aaaaaatgcg
    11641 attagaccgc ttgaagagac ttctttagaa gccttaacca cgaataccgt attttcgatt
    11701 ttgcggaatg cgaaagcgat tgaaatgaat accgacccaa atttggtggt gtgctgggga
    11761 gggcattcga ttaatgaaac cgaataccaa tattgccgtg ccgtcgggca agagtttggc
    11821 ttgcgtgagc tgaatattgt gaccggttgc ggagccggtg tgatggaagc cccgatgaaa
    11881 ggagcggcta tcggacacgc caatcaacgt tataaaaacg ggcgttttat cggcattacc
    11941 gagccgtcta ttattgcctc cgagccgcca aacccgattg tgaatgaatt ggtaattatg
    12001 ccggatattg aaaaacggtt ggaagccttt gtgcgtttag ggcacgccat tgtggttttc
    12061 ccgggcgggc cgggaacgct ggaagagttg ctgtatatta tcggtattaa gttaaacccg
    12121 gctaaccatg ctcagcaatt accactgatt ttaaccgccc cgaaagaatc ggcagactat
    12181 ttctatgcca tcgatcaatt tattggcgaa actttaggcg aagaagctcg cagtttgtat
    12241 gaaattatta ttgatgactc ggtgttggtg gctcgtaaag tcaagcaagc gatgattgag
    12301 gtaaaaagca gccgttatga aagtggcgat gcttataatt tcaactggtc gttaaaaatt
    12361 gaggaagatt ttattacgcc atttattcca acccacgaaa atatggcaaa cctgaattta
    12421 agtttagcac agccaaaaga gcagctggct gcgaatttaa gacgggcatt ttcagggatt
    12481 gttgcaggta atattaagcc ggagacctcc gatttaattg agaaatatgg ggtgtttaaa
    12541 ttacacggcg atcgtaaatt aatggagcat atagataaac tgttggagcg ttttgtggtt
    12601 caacaccgta tgaaaattcc aacgggtgag gcgtaccaac cttgctatga aattgtaaaa
    12661 gggtaatgct aacaaggggt aaggtatgta tttaaagcat attgaaatca gtggctttcg
    12721 tggtattaac catttatcgc tccagctacg accaaatatg gtattgattg gcgaaaatgc
    12781 gtgggggaaa tccagtctga ttgatgcatt aagcctgatt tttaaccttg agcaggcgtt
    12841 gtatcaattt cagttcaaag atttccatat tcaatacacc gataccccca accaagtgcg
    12901 ggacattcaa ctggtgttta ccttttgtga agagcatcag cacgatttag cggtgcaatt
    12961 tcccgcttat ttggcacaag ccgcgtttta tattgataat caccgctgtg tttcgcttgc
    13021 cgtaaccgga aaattacacg gagagcagat tagcaccgac tatcaatttg tggatcaaag
    13081 cctaaaaccg atccagatag aacaagcgga aaacgccatt cggcagttta tttaccatca
    13141 ccctgtttat cgcctgcgtg atgcaaggct caataacagg gtgcataatg aattattact
    13201 taaaggatcg tctcaacttg aagacgattt tcaacgtgaa ttaaacgccc tctcttcgct
    13261 actgaaatac tattttctaa ccagccaaag tcgctatttg atcaaaacca gtatgcaaga
    13321 caccgcattg tggtgggaaa aagtaaaatc cctctgttta cgcctaaaac agcagccgga
    13381 attggtcgaa tctgctcagc agcatatttc tactctattt ttgtttaatc ctagatctac
    13441 agtaatagaa aagccgatta ttttatttga agatctgaat gctcaactac accctcgaat
    13501 gattgcgatt ttttgggaac tgctcggttt tttacccagc caacggatca ccactactaa
    13561 ttctattgaa ttgttatcgc aagtgcctct gcgtgaaatc tgccgtttgg tgcgtcatca
    13621 cgatcatacc caagcctatt ggcttgaacg ccacagttta ggcaaggaag atttgcgaaa
    13681 attaaccttt catattcacc ataatcgtgg cttggcactg tttgctaaag cgtggatttt
    13741 ggttgagggg gaaacggagg tttggatttt gcaagagctg gcgagattat tagatattca
    13801 tttggaaatg gaagggatca aaatcgttga atttgctcag tgtggcttac gtccgttaat
    13861 taaatacgcc agagcaatgg ggatcgaatg gtatgtgctg actgacggtg acgattccgg
    13921 caaacgttat gcagatacgg taaaaagtat ggaagaggat tttgctcacc ggctcacgat
    13981 gttgccacaa aaagatatcg aacatttttt ctacaaagca ggcttgcgga atgtgtttat
    14041 acagcttgca aattggcgtt cacaagaaaa atatccggtg tcctacatca ttaaacgagc
    14101 gattcaacga agctcaaagc ctgatttagc cattgcatta gccaatgaga tggggcaacg
    14161 gggagagcat tcaattccaa ctttatttaa acaattattt gcagatgtgc tggcattgat
    14221 aaacaaagta tagaatacaa gcggttaaat ttgacaaaaa attgccaatt ttgaccgctt
    14281 gtttgcttgt gatgaaaacc aatcatacaa aactctaatt tatttcaagt gcaagctatt
    14341 attttatgcg ttatgcttat ttggaataaa aaaatcgggt tagttgattg acatagcatt
    14401 ctagacgtct aaaataacca aaaataatag ttaattgaat gtggataaat agaaaatgat
    14461 tcagctgaaa aatatcagca aacaatttga ggtaaagggc aaaaaaatta ccgctctgga
    14521 taatgtaaac ctagaagtac caagaggcac tatttatggt gtcattgggg catcaggggc
    14581 aggaaaaagt accttaattc gctgtgtaaa tttattagaa cgcccaacaa tcgggcaagt
    14641 gattgtcgat gggcaagatt taacggcaat gtcggataaa gagttgattc acgctcgccg
    14701 taatattgca atgattttcc aacattttaa tttgctttca tcacaaactg tatttgaaaa
    14761 cgtggcgtta tcgttgcgtt taagcaatac gccaaaagag caagttgaga agaaagtcaa
    14821 tgagctgttg gatttagtgg ggttaacaga tcgtaaagat gtttaccctg caaatctttc
    14881 cggtgggcag aaacagcgtg tagcgattgc tcgtgcgtta gccagcgatc cacacgtttt
    14941 gttgtgtgat gaagcgacca gtgcgttaga tccggcgact acgcaatcca ttttgcagct
    15001 attaaaagat attaataaac gtcttggttt gacgatttta ttaatcactc acgaaatgga
    15061 agtggtaaaa cgcatttgtg accgagtagc agtgattgat aaagggcagt tgattgaatc
    15121 tggctcggta agtgaaattt tctcgaatcc gaaaaccgaa ttagctcaga aatttattca
    15181 atcgactttc cattttgaat taccgcaaga atataccgag cagctttcaa gttcgccgac
    15241 tgaacatagt aagccgatta ttaaatttga gtttaccgga cgttcggtgg atgcaccttt
    15301 gctttcaatg gcatcgaaga aattcggcat tgattttagt attttgatgt cgcaaattga
    15361 ttatgcaggc ggggtcaaat ttggatttgt aattgcagaa gtgagtggta atccggattc
    15421 cattgcagag gcaaaatttt atttaattga taacaatgta aaagtagagg tgttaggcta
    15481 tgtgggctga ttttatgcag gagatgacgc cacagatgtg gagcttagta tgggaatcta
    15541 cttttgaaac cgtttatatg ggctttgtgg caacggcatt ggcggtagtc atcggtttac
    15601 cgattggttt cttggcgttt ttaaccggta aaggcgaaat tttagaaaat ccgagattac
    15661 accaagtgtt agatgtcgtt attaacatcg ggcgttcgat tccgttcatt attttattga
    15721 ttattttgtt gccatttact cgcttagttg tgggcacaac attaggcaca acggcagcga
    15781 ttgttccttt aagtgtttcg gccattccgt tttttgcccg tttaacttca aatgcgttgc
    15841 ttgaaattcc gtccggttta acagaagcgg caaaatcaat gggagcaaca aattggcagg
    15901 tagtgacaaa attttattta ccggagtcat taccgatttt gattaatggc attacgctaa
    15961 cgctggttgc tttaattggc tactcagcaa tggcgggggc tgttggtggc ggcggtttag
    16021 gcaatttagc gattagctat ggcgaacacc gtaatatggt gtatgtgaaa tggattgcta
    16081 ccattattat tgttgcgatt gtgatgatta gccaaaaatt gggtgatcat ttagcaaaac
    16141 gttacgatca tcgttaattt tatttaaaaa gaggtttcta tgagtttcaa gaaaatttta
    16201 ggcgttgcat tggtttctgc attagcatta accgcttgta aagaagagaa aaaggcagaa
    16261 tctaccgctg ctccggcagc tcaagctccg gctaaaatca aagtaggggt aatgtcaggc
    16321 cctgagcata ccgttgcaga acgtgcagca cagattgcaa aagaaaaata tggtttagaa
    16381 gttgagttcg ttttatttaa cgactacgct ttaccgaata ctgcagtaag taaaggtgat
    16441 ttagatgcaa acgcattcca acacaaacca tatttagata aagacagcca atcaaaaggt
    16501 ttaaacaact tagtgattgt gggtaacacc tttgtttatc cgttagcggg ctactctaaa
    16561 aaagtgaaaa atgtgtctga gttagcagaa ggtgcggtaa ttgcagtacc aaatgatcct
    16621 tcaaacttag ctcgtgcttt gattttatta gaaaaacagg ggttaattaa attaaaagat
    16681 aataccaatt tattctcaac ttcagtggat attatcgaaa atccgaaaaa cttaaaaatt
    16741 aaagaagtgg atacttcaat tgccgcaaaa gcgttagatg acgtagattt agcggtggta
    16801 aataacactt atgccgggca ggtaggctta aatacccaag atcacggtgt atttgttgag
    16861 tctaaagatt caccgtatgt gaatattatt gtggctcgcc aagacaataa agatgcagct
    16921 aatgtacaaa actttattaa atcttaccaa accgaagaag tgtaccaaga agcacaaaaa
    16981 cactttaaag atggtgtagt aaaaggttgg taatttagct gatttttact taaatggcga
    17041 ttaagcgcta ggttaatcgc cattttcatc tttaaaatat aggaaggagc aaacaatgaa
    17101 ctttaaaaaa ttattaggtg tggcgttagt atctgcctta gcacttactg cgtgtaaaga
    17161 tgaaaaagca caagcacctg ctacaacagc taaaactgaa aacaaagccc cattaaaagt
    17221 gggtgtgatg accggccctg aagcccaaat gaccgaagtg gcagtgaaaa ttgcaaaaga
    17281 gaaatatggc ttagatgtag agttagtgca gtttactgaa tacactcaac caaatgccgc
    17341 acttcattct aaagatttag atgctaacgc gttccaaacg gtgccttatt tagagcaaga
    17401 agtgaaagat cgtggttata aattagcgat tatcggcaat acgctagtat ggccaatcgc
    17461 ggcttattct aaaaaaatta aaaacatttc cgagttaaaa gacggagcga ctgttgcgat
    17521 tccaaacaat gcaagtaata ctgctcgtgc gttattattg cttcaagctc acggtttatt
    17581 gaaattaaaa gatccgaaaa atgtgtttgc taccgaaaac gatattatcg aaaacccgaa
    17641 aaatatcaaa atcgtacagg cggatacctc acttttaacc cgtatgttag atgatgtaga
    17701 acttgcggta atcaacaaca cttacgcagg tcaagctggg ttaagtccgg ataaagacgg
    17761 tattattgtg gaatctaaag attcaccgta tgtgaattta gtggtaagtc gtgaagataa
    17821 taaagatgac ccacgcttac aaacttttgt gaagtcattc caaaccgaag aagtattcca
    17881 agaagcgtta aaattattta acggtggtgt ggtgaaaggt tggtaatcag ttggacggca
    17941 tattctgccg tccttttctt aaaaggagaa acagataatg aaaataatga aattagccgg
    18001 tgcagttgca attttctcgc tttttttaac cgcttgtaat gataaagccg aaaagttgaa
    18061 agtcggtgtg atttccggcc ctgaacataa ggtaatggaa gtggcggcaa aaattgcaaa
    18121 ggaaaaatat aaccgtgatg ttgaattagt ggtatttacc gattatgcca cgcctaatgc
    18181 agctttagat aaaggcgatc ttgatttgaa tgctttccag cataaacctt atttagataa
    18241 ccaaattcag gaaaaaggct ataaattagt gccggtcggc aatacttttg tttatccgat
    18301 tgcggcttat tccaaaaaaa ttaaatcgct ggcagagttg aaagatggtg atactattgc
    18361 agtgcctaat gatccgacta atttagcccg tgctttaatt ttattggaaa aacaagattt
    18421 aattaagctg cgagcagatg caggcttaaa agcaaccagt gtggatatta ttgaaaaccc
    18481 tcgtaaattg gtgatccaag aaattgaagc accattattg cctcgaacgt tggacgatgt
    18541 tgccttttcg attattaata ctacctacgc agggcaaaac ggtttaacgc caaccaaaga
    18601 cggaatattc gttgaagaca aggattcgcc ttatgtgaat ttaattgttg cccgtgaaaa
    18661 taaccaacat tctgaagccg taaaagattt ggtgaaagcc taccaaacag aagaggtgta
    18721 taacaaagcg aatgaagagt ttaaaggggc gatgataaaa ggctggtaat tgaagataaa
    18781 taaggcatag attcctatgc cttacttttt atgttacaga cctgctccgg ctcttaatgc
    18841 ttcggctttg tcagtttttt cccaaggaaa atgtttacga ccgaagtgtc cgtaggcagc
    18901 ggtttcacgg tagattggtt tgattaaatc taacattttg attaagccat acgggcgtaa
    18961 gtcaaagttt tggtagatta atttcacaag tatttcgttg ctcactttac ctgtgccgaa
    19021 ggtttcgacc ataattgatg ttgggtcagc caccccgata gcataagaaa gttggatctc
    19081 acagcgatcg gctaagcctg cggcaacgat attttttgcg acataacgag cagcataagc
    19141 agcagagcga tccacttttg acgggtcttt accagagaat gcaccaccac cgtggcgagc
    19201 cgcaccaccg taggtatcta caatgatttt acgcccggtt aaaccacagt cacccattgg
    19261 accgccaatc acaaaacgac cggttgggtt gatgaaatat ttggtgtttt ggctgagcca
    19321 ttcgcttggt agtaccggtt tgatgatctc ttccatcacc ccttcaatta actctttttg
    19381 cgaaatatgc tctgcgtgtt gtgtagaaag cactacggca tcaatgccga taattttatt
    19441 gttctcataa gcaaatgtta attggctttt agagtcaggg cgtaaccaat cgagtttgcc
    19501 cgatttacgc acttttgact gctgttccat taagcggtgt gcgtaagtaa ttggagccgg
    19561 cattaacacc tcggtttcat tggtagcgta gccaaacata atcccttggt cgcccgcacc
    19621 ttgctctaac ggatcggcac ggtcaacgcc ctggttgatg tccggagatt gtttaccaat
    19681 cgcatttaat accgcacaag agtgagcatc aaagcccata tcagagtgag tatagccgat
    19741 gtcgcaaatc acttggcggg ttaagttttc aatatccacc cacgcagagg tagtgatttc
    19801 tccccctact aatgccatac cggttttcac ataagtttca caagcaacgc gtgctttagg
    19861 gtcttgtttt aaaatttcat ctaataccgc gtctgaaatt tggtctgcga ttttatccgg
    19921 atgcccttct gaaaccgatt cagaagtaaa taaattaatt gccatttgta ttcctctcta
    19981 tcttaaata
//
LOCUS       DANLUQ010000030        19917 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.030, whole genome shotgun sequence.
ACCESSION   DANLUQ010000030 DANLUQ010000000
VERSION     DANLUQ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 19917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19917
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.030"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(90..728)
                     /locus_tag="PXB27_000850"
     CDS             complement(90..728)
                     /locus_tag="PXB27_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDL5345667.1"
                     /translation="MRYTTEQIIEKLRQLKVVPVIALDKAEDILPLAKTLSENGLPVV
                     EITFRSEAAEEAIKLVQANYPEVLIAAGTVLTKEQVLTAKNAGADCIVTPGFNPTIVK
                     YCQVLGLPVTPGVNNPMAIEAALELGIQAVKFFPAEASGGVKMIKALLGPYGNLQIMP
                     TGGISPQNINDYLAIPNVVACGGSWFVEKSLIKEQKWEEIGKLTKEVVELVK"
     gene            complement(816..1763)
                     /locus_tag="PXB27_000851"
     CDS             complement(816..1763)
                     /locus_tag="PXB27_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDL5345668.1"
                     /translation="MKKLAILGECMIELNGEPFGEMHQTYGGDSLNTATYLARVSSPE
                     QIEVRYISALGTDKLSQGMLAAWQVDNINTDFVLRDEHRQPGLYLIQLDKQGERTFLY
                     WRNQSAARYLLQHPGYPKALSALNEVDMVYLSGISLAILPENDRTLLIAQLRELKTQG
                     VKIAFDSNYRPKLWESVAAAQAHYAELLQLVDLALVTFDDEQLLWGDVNEQATFDRLT
                     QYGIQTIVVKQGSLGATVLQNGERTFVPTVAVEKVVDTTSAGDSFNAGFLNGYLLGKD
                     VVTCCQQGNAVAGIVIQHKGAIIAKSATTHLKSQFEQSI"
     gene            complement(1780..2625)
                     /locus_tag="PXB27_000852"
     CDS             complement(1780..2625)
                     /locus_tag="PXB27_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL5345669.1"
                     /translation="MSIAQNHNFKDKVVVITGAGGVLCAYFAKEIAKTGAKVALLDIN
                     LEAAQNFASEIADEGYIAKAYQTNVLELESIKQARDEILKDFGTCDILINGAGGNNPK
                     ATTDNEFHDINLPENTKSFFELDKAGIEFVFNLNYLGTLLPTQVFAKDMLGKEGANII
                     NISSMNAFTPLTKIPAYSGAKSAISNFTQWLAVYFSKVGIRCNAIAPGFLVSNQNKAL
                     LFNADGTPTARANKILTNTPMGRFGQADELVGAILFLMDHHYSSFINGVVLPVDGGFS
                     AYSGV"
     gene            complement(2637..3908)
                     /locus_tag="PXB27_000853"
     CDS             complement(2637..3908)
                     /locus_tag="PXB27_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HDL5345670.1"
                     /translation="MLWLFLGSLFLFLFLGVPVALSMLLSASFMLWKMDMFDAQLITE
                     NFVMGTNNFPLMAIPFFMLTGEIMKHGGISERIINFAMSMVGHIKGGLGYVVIISGLI
                     FAGLSGSAVADTAALGAILIPMMVARKYDVARSTGLICAAGIISVVIPPSIPMIVYGI
                     TAGASITKLFMGGMVPGFLMVLGLWLVWKFLYRNEEQTVEPKKTGKERWAAFKSAFLP
                     LLLPIIIIVGLRGGIFTPTEAGVVAAIYAAIISIILKGLTLDKIQDVFLGTIKTTAMV
                     MFVAAAAMISAFAVTVAQIPMELVEFIKGITDSPTVLMFVIMIFLLLVGCVMDLIPAV
                     LIFVPVLLPLLRAYNIDIAYFGIMMVINLSIGLITPPVGTVLYVGSGIAKIGIARLTK
                     GIYPFLLVYAAILFLLILFPEIIIVPMNWLS"
     gene            complement(3911..4390)
                     /locus_tag="PXB27_000854"
     CDS             complement(3911..4390)
                     /locus_tag="PXB27_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDL5345671.1"
                     /translation="MQISTALDRTLKLLAIIALSTMIILVFFNAVLRYFFDSGIAWSE
                     EFARICFVYMIFLGIILVAKERGHLTVDIVVSALPAKVQMYLLFFADILVLVALSFIT
                     YGAYQLMLLTYTQKMPATEISSAFLYFAAVISSISYFAIVFTNLWKHIRLAFIKEEK"
     gene            complement(4451..5434)
                     /locus_tag="PXB27_000855"
     CDS             complement(4451..5434)
                     /locus_tag="PXB27_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDL5345672.1"
                     /translation="MKLKTFTKALLISSLMFTGSAFAKTIIKIGHYNSDVHPSNIALQ
                     EYFKKTIEEKTEGRYEIRIFPNNQLGGEDQIVNGLRNGTIEGGITGLLLQNVDPIFGV
                     WEWPYLFKDNAEAKKVLESPIAQEIGEKMEKYGIKLLAYGMNGFRVISSNKKVENFDG
                     FKGLRLRVPLNSLFVDWAKAMHINPQSMPLSEVFTALEQKVIDGQENPYMLIKDSGLY
                     EVQKYIIESNHIFSPGLLQLSLKTWNKMSKEDQTIFLEAAKLYQEKEWELAMKMEQDV
                     KDFFHKNGKEVIVPSEQFKADMLKASETLYQNFYQKYDWAKDVISKIHAAK"
     gene            complement(5463..6488)
                     /locus_tag="PXB27_000856"
     CDS             complement(5463..6488)
                     /locus_tag="PXB27_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDL5345673.1"
                     /translation="MEKPLKSVCLVEPNKVGIQTVSYPVKGENDVLIKVEGMGICGSD
                     IGAFRGTNPLVTYPRILGHEIVGTVIESGKGMPNNINVGDRVIIEPYIYCGHCYPCSI
                     GRTNCCESLKVLGVHIDGAMQEVVRHPAHMIIKTPDMPIHELALAEPLTISLHAIHRT
                     KVKAGEHVAIIGAGAIGLMAALVAKAYGATPILIDILDKRLDYAKSIGIPHTINPTKE
                     NDLEAIKSITNGRMAEVVIEASGANVAVQNTLKYTSFAGRIALTGWPKTETPLPTNLI
                     TFKELNIYGARTSKGAFEEALELLASRRIEPKDIISKVITFDEIPHYIEELSANPDDY
                     LKIIAVF"
     gene            6652..7413
                     /locus_tag="PXB27_000857"
     CDS             6652..7413
                     /locus_tag="PXB27_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HDL5345674.1"
                     /translation="MQNSSRTYTKIGKILKEEIAQGKYRVGDKLPTERDISERFEVSR
                     TIVREAIVMLEVEKLIEVKKGSGVYVIRVPELFPEDFPEEKDNELRDVGPFELLQARQ
                     LLESSIAEFAAQQATKQDIQVLRNILESEKKLLLEKMDDYSADKEFHLALAHMTGNDV
                     LIQLQEQLWRYRFNSAMWAQLHSRILQHDHHHLWIEDHERILNAIQRKNPALARKAMW
                     QHLENVKTKLLELSDVEDPKFDGYLFNVNPVVVGI"
     gene            7443..8627
                     /gene="uxuA"
                     /locus_tag="PXB27_000858"
     CDS             7443..8627
                     /gene="uxuA"
                     /locus_tag="PXB27_000858"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012341261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL5345675.1"
                     /translation="MEQAWRWYGPNDPVSLDDVRQAGATGIVTALHHIPNGQVWSVEE
                     INKRKALLAEKGLVWSVVESVPVHEEIKTQTGNYQQWIDNYKQTLRNLASCGIDTVCY
                     NFMPVLDWTRTDLEYEMPDGSKALRFDQVAFAAFELYILKRPGAEATYSSAEQAEAKA
                     YFEQMSDADIAKLTKNIIAGLPGSEEGYTLEEFQAQLDRYQGLSAEKFRTHLAYFLNE
                     IVPVAQEVGIRMAIHPDDPPRPILGLPRIVSTIEDMQWFVETQPLAANGFTFCTGSYG
                     VLAENDLVKMAEQFADRIYFAHLRATQREENPQSFHEADHLAGDVDMFGVVKALLTEE
                     YRRKANGDHRLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLRGLAEFRGLELALKKVYF
                     ND"
     gene            8678..9547
                     /locus_tag="PXB27_000859"
     CDS             8678..9547
                     /locus_tag="PXB27_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL5345676.1"
                     /translation="MFKKYFGEIILFLVTFIAAAGWFFSKHALVGLPPIGFMGGRFIL
                     ASLILLPFSYRELYRLDKVQVKYSVITGLTYGIYTIFWVLGLAFSTVFGEGAFLVSLA
                     MLIAPLLSWLIFKHTPPKIFWLALPIALSGLYFLSAEKGIFHFSLGSTIFLVSSVFGA
                     LYFVLNNQYAKQVSLFPLITIQLAIVGAVCSLYSYFMESWHFPILTEVWLWFAASVLI
                     ATNLRFLMQTLGQKYCHIGTSALIMLLEPVWTLLLSVMLLGEQLSATKWLGCSLILIA
                     LIIYRLPILRLKK"
     gene            9715..10029
                     /gene="rsfS"
                     /locus_tag="PXB27_000860"
     CDS             9715..10029
                     /gene="rsfS"
                     /locus_tag="PXB27_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDL5345677.1"
                     /translation="MEQNLVEFLTKTLDDLKAQDILAIDVKGKSSITDTMILATGTSS
                     RHVASTAERLITEAKQAGFEVFGDEGKAVADWIVVDFGKAMVHLLQSDSREMYQLEKL
                     WA"
     gene            10106..10573
                     /gene="rlmH"
                     /locus_tag="PXB27_000861"
     CDS             10106..10573
                     /gene="rlmH"
                     /locus_tag="PXB27_000861"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010618862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDL5345678.1"
                     /translation="MKIQLIAVGTKMPAWVTTGFEEYQRRFPKEMPFELVEIPAGKRG
                     KNADTKRILELEGKAMLAAAGKAKIVTLDIPGKAWTTEQLATQLEGWKNDGRDVALLI
                     GGPEGLSPECKAAAEQSWSLSPLTLPHPLVRVVVAESLYRAWSVTINHPYHRE"
     gene            10597..12546
                     /gene="mrdA"
                     /locus_tag="PXB27_000862"
     CDS             10597..12546
                     /gene="mrdA"
                     /locus_tag="PXB27_000862"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDL5345679.1"
                     /translation="MFGKLAKFTASNQREKVRDGQAESNLYARRALVAFLGVLALSGV
                     LLTNLYHLQVVNYEDYQTRSNGNRIKLLPVPPARGLIYDRNGKVLAENLTYFGLFIVP
                     EKTENIEQTLTELKDIVGLTDEDIENYHKEKKRSSRYTPIMLKGNLNEAQMARFAVNQ
                     YRFPSLDVQAYYKRNYPYGELLTHIIGYVAKINENDKKKLQEAGKFGNYAGSHDMGKL
                     GIEKFYEDQLHGVTGFEEVEINNRGKVIRKLRDQAGVAGSSIRLSIDVELQQYVMSLI
                     AKHKGAVVVLDPRDSSILAMVSNPSYDNNLFVGGISGQDYKALLDDPTRPLYSRTTQG
                     TYPPASTIKPFMAVAGLEEGMITPSTTISDPGYWILPGTTQRYRDWKRSGHGATNVNK
                     AITESSDTFFYQLAYKMGIDKMSDWMKKFGFGVKTGIDIFEESAGVMPTREWKQKRYK
                     KPWVQGDTIPVGIGQGYWISTPLQLAKATAVLINNGRVNTPHLMLEVQSSNSVTPYQD
                     PLLYEDIKGVPDVYWNLAKQGMYNVNHAANGTARKVFAGAFYHSAGKTGTAQVFNLNG
                     KNYDKNAIKKELHDHAWFIGYAPYEQPKIVVALILENAGGGGSAAAPVARQIMDYALR
                     KGIGEQPDSQNSEQNKPLVTEEHHE"
     gene            12539..13666
                     /gene="rodA"
                     /locus_tag="PXB27_000863"
     CDS             12539..13666
                     /gene="rodA"
                     /locus_tag="PXB27_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HDL5345680.1"
                     /translation="MNSGFKKLFWKIFSLDLLLLSGLLAITGYGLLVLYSASGANERM
                     FTSRLIQVSLGLGLMLIMAMVPPRFYQKVSPYLYIFCLILLILVDLVGETSKGAQRWL
                     NLGFVRFQPSEIAKLAVPLMVATYLGKRPLPPSFKDTFIALAIIIVPTLLVAIQPDLG
                     TSILVCSAGIFVLFLAGLSWKLIGAGVVFLAGFIPIMWFFLMHDYQKTRVMTLIDPNK
                     DPLGAGYHIIQSKIAIGSGGINGKGWMEGTQSQLEFLPEPHTDFIFAVLSEEHGMIGV
                     LILLAIYLFIIARGLVIGTKSDSAFGRILSGGTALLFFVYVFVNIGMVSGILPVVGVP
                     LPLFSYGGTSYVTLMAAFGLMMSTYVHRKRVGSNNPYDKLK"
     gene            13758..14417
                     /locus_tag="PXB27_000864"
     CDS             13758..14417
                     /locus_tag="PXB27_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HDL5345681.1"
                     /translation="MRLGKIVTLLLAGFLTFSSLNSLAATKNTKAKSSILAKKQATSK
                     AIKKVGISKVKKKEVVAKATKKKTISTVTTKPIKSTKKLARKVVKKTVTAKKKLVKKQ
                     HKPLVKKHFQTGVASYYANYFNGRRTANGETFNNAKMTAAHRTLPFGTLIEVTNLRNG
                     RSVVVRVNDRGPYAHARVLDLSSAAAKKIGMHHSGTARVKIAIVDKSKLGSTNQFYVM
                     N"
     gene            14431..15609
                     /locus_tag="PXB27_000865"
     CDS             14431..15609
                     /locus_tag="PXB27_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HDL5345682.1"
                     /translation="MKKTLVKAMSVVGFAVSSIAYADLNTNFNLSAPQINAQTYILMD
                     YNSGEVLVSLNPDQRQYPASLTKMMTSYVVGEALKSGKVQNSDMVIVTENSWAQKFPG
                     SSLMFLDLNSKVSVGELMKGLIIVSGNDASVALAEHVSGSQAAFINEMNKYAQQFGLK
                     NTHFTTVHGLDDPEQYSSARDMAIIGSHIIRDQPEEYKIYAEKEFQYNIKKPQPNRNG
                     LLWDKTIQVDGMKTGHTDKAGYNLVASAINPNTRLISVVMGVPTYKGREVESKKLLQW
                     GFANFETIKSIPAAQSVAEQSVYYGEVNKVQLGSVQDSFVTIPKGRSADLKARYELEK
                     KNLEAPLAKGQVVGKMIYQLDGKDVASTNLQVLQDVPEAGIFGKAWDWIALTVKSLFD
                     "
     gene            15777..16076
                     /locus_tag="PXB27_000866"
     CDS             15777..16076
                     /locus_tag="PXB27_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HDL5345683.1"
                     /translation="MSQPKAINLQDLPQAKLKDLLEFPCHFTFKVVGANREDLVDDVV
                     VVTQKYAKGDYNPRQQKSSKGTYNSVSIDIVAENIEQVETLYTELAKINGVRMVL"
     gene            16138..16791
                     /gene="lipB"
                     /locus_tag="PXB27_000867"
     CDS             16138..16791
                     /gene="lipB"
                     /locus_tag="PXB27_000867"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HDL5345684.1"
                     /translation="MKLIIRQLGIQPYEEIWHQMQSFTDSRDGNTDDEIWLVQHPAVF
                     TQGSAGKPEHLLNPTNIPVVQSDRGGQITYHGEGQQIMYVLIDIKRLKSLGKEVSVRD
                     LVTALEQTVVQTLAEYGIESYPKADAPGVYVDGKKICSLGLRIRKGCSFHGLALNINM
                     DLTPFQNINPCGYAGLEMCQLSELVHRPDLRCEEVAPKLVAHFSTILGYNNQQIINN"
     gene            16806..17813
                     /gene="lipA"
                     /locus_tag="PXB27_000868"
     CDS             16806..17813
                     /gene="lipA"
                     /locus_tag="PXB27_000868"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HDL5345685.1"
                     /translation="MATSTAQTTATATVKKMSPFKMEKGVKYRDAAKTSVIQVRNIDP
                     DQELLKKPEWMKIKLPANSAKIDSIKNGMRRHGLHSVCEEASCPNLHECFNHGTATFM
                     IMGAICTRRCPFCDVAHGKPLPLDPDEPRKVAETVQDMKLKYVVITSVDRDDLPDRGA
                     AHFSATVREIKALNPHCKVEILVPDFRGRVEQAVAILKENPPDVFNHNLENVPRLYRE
                     VRPGADYKWSLELLKIFKQEFPHIPTKSGLMVGLGETNEEILEVMQDLRDHGVTMLTI
                     GQYLQPSRHHLKVERYVPPAEFDMFREEAEKMGFEHAACGPFVRSSYHADLQAKGELV
                     K"
     gene            18000..19103
                     /locus_tag="PXB27_000869"
     CDS             18000..19103
                     /locus_tag="PXB27_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5345686.1"
                     /translation="MKKLLLNSVFAATLLAFGATANAEIKTITDVLGREVKVDVPAKR
                     VALTFYYPDYIAVTGVENFDKVVGISREFWEKFNPGSWALFAEKMPNLKNIADLGYVN
                     SGTFSTEKTIALKPDVLVLPEIQYQALASEISRIEQAGIPVVVDFNKQTIENHTKSVR
                     VFGQLTGTEERAEKIAKEYEEGIADIQSCIDNAKVSKPKIYAEFGNKGPKEHSFTFGK
                     NMWGAIAETVRGDNISAPFVENWGQINPEQVLVSKPDVIMISGTELGNDTNPEIMSMG
                     INIEEADAQKRLKGFIERTGWKELPAVKSGRVYGLYHTASRSISDLASAQFMAKALYP
                     ELFKDIDPEKTYLDFHNNYLPITPKGTFFMQLK"
     gene            complement(19194..>19917)
                     /locus_tag="PXB27_000870"
     CDS             complement(19194..>19917)
                     /locus_tag="PXB27_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL5345687.1"
                     /translation="VVADKINLHFEQGKIYTLLGPNGAGKSSLLKTLFGEIAHQGTID
                     FDGNKLTSSHLHSWRKEIGYMPQDTQVEASLSALEVILLGQLDSLNMYISDELLNEAV
                     DIMDKLGISHLAHQDIMRLSGGQRQMVMFAQVLLRKPHILLLDEPVSALDMYHQINLL
                     EYVNAYTRANNLITIMVLHDLSLAAQFSDSVMLLSEGKFKHKAELVICWNAILFRDSI
                     MSILRFYMMETARRLSARFANV"
BASE COUNT         5941 a         3836 c         4319 g         5821 t
ORIGIN      
        1 aagtatgcaa tatttgttat gctttgtaaa taagaggaaa gagatccatt ttgggtagaa
       61 aatagctcat tgcttggaac tttaaatggt tatttcacca attcaaccac ttccttcgtc
      121 aatttcccaa tctcctccca cttctgttcc ttaatcaggg atttctccac aaaccaagag
      181 ccgccgcagg cgactacgtt gggaatggcg aggtagtcgt tgatgttttg tggcgagatg
      241 ccgccggtcg gcataatctg taagttgccg taagggccga gcagggcttt aatcattttc
      301 acgccgccac tggcttcggc agggaaaaat ttaactgctt gtatgccaag ttcgagggcg
      361 gcttcaatcg ccatcgggtt gttcacaccc ggtgtaacag gcaaacctaa cacttggcaa
      421 tacttaacga tagtcggatt aaaacccgga gtcacaatgc aatccgcccc agcgtttttg
      481 gcagttaaga cttgctcttt ggtcagcact gtgcctgctg caatcaatac ttccggatag
      541 ttggcttgca ctaatttaat tgcctcttca gctgcttccg agcggaaagt gatttccacc
      601 accggcaaac cgttttcgga aagggttttc gccagcggta aaatatcttc tgctttatcc
      661 aaagcaatca cgggaacaac tttcagttgt cttaattttt caatgatttg ttcggtggtg
      721 taacgcattt ctagcctcat tatgttgtat tgcaagcggt cgcttttgta aaaaattttg
      781 caaatttaac cgcttgtcgt tgtgctattg ttggtctaaa tggattgttc aaactgcgat
      841 ttcaaatgag tagtggcgga tttggcaata atcgcccctt tgtgctgaat gacgataccg
      901 gcaacggcat ttccttgttg gcaacaggta acgacatctt tacccaataa gtaaccgtta
      961 aggaagccgg cattaaagga atcgccggcg gaagtggtat cgaccacctt ctctaccgca
     1021 acagttggca caaaagtacg ctccccgttt tgcaacaccg ttgcccccag agagccttgt
     1081 ttcaccacaa tggtctgaat gccatattga gtgagacggt caaaggtggc ttgctcattc
     1141 acatcgcccc aaagcagttg ctcgtcatca aaggtcacga gagctaaatc gactaattgc
     1201 aacagctcgg catagtgagc ttgggcagca gccacacttt cccacagttt cggccggtaa
     1261 ttgctgtcaa aggcgatttt caccccttgt gttttgagct cacgcaactg tgcaatcagc
     1321 aaagtgcggt cgttttccgg caagatggca aggctgatac cgctcaagta aaccatatct
     1381 acctcgttta gtgccgagag tgctttggga tagccggggt gttgcaacaa ataacgggcg
     1441 gcagattgat ttcgccaata gaggaacgtt cgctcgccct gtttatccaa ctgaatcaga
     1501 tacagccccg gctggcggtg ctcgtcacgc aacacaaaat ccgtgttgat attatccacc
     1561 tgccacgccg ctaacattcc ctgactgagc ttatccgtgc cgagagcgga aatataacgc
     1621 acctcaatct gttcaggcga actcacccga gccaaatagg tagcggtgtt gagcgaatcc
     1681 ccaccatagg tttggtgcat ctccccaaac ggctccccgt ttaactcaat catacattca
     1741 ccgagaatgg cgagtttttt cataggaata ccttctaagt tacacgccgc tataagcaga
     1801 gaatccgcca tcaacaggta atacaacgcc attgataaag ctggaataat ggtgatccat
     1861 taaaaatagg attgcaccta ccaattcatc ggcttgtccg aaacgtccca taggggtatt
     1921 cgtgaggatt ttattagctc tagctgttgg cgtaccatcg gcgttaaata gtagagcttt
     1981 attttgatta ctcactaaaa atcccggtgc aatagcatta cagcggatac ctacttttga
     2041 gaaataaact gctaaccatt gtgtaaagtt actgattgca gattttgctc cagaataggc
     2101 agggatttta gttaatggag taaaggcatt catactggat atattaatga tattagcgcc
     2161 ctctttaccc aacatatctt ttgcgaaaac ctgtgttggt aatagtgtgc ctaagtaatt
     2221 taaattaaat acaaattcaa tccctgcttt atctaattca aaaaaagatt ttgtattttc
     2281 cggtaagtta atatcgtgaa attcattatc tgtcgttgct tttggattat tcccacctgc
     2341 accattaatt aaaatatcac aagtgccaaa atcttttaga atttcatctc ttgcctgttt
     2401 tatgctttct aattcaagta cattagtttg gtacgctttc gcaatatagc cttcatcggc
     2461 aatttcagaa gcaaaattct gtgctgcttc tagattgata tcgagtagtg caacttttgc
     2521 acctgttttt gcaatttctt ttgcaaaata agcacatagc acaccacctg ctccggtaat
     2581 aacaacaact ttatctttaa aattatgatt ttgggcaata gacataataa gaactcctaa
     2641 gataaccaat tcattggtac gataataatt tcagggaaaa gtattaataa gaacagtatt
     2701 gcagcataga ctagcaagaa tggatagata cctttggtta atctagcaat accaattttt
     2761 gctataccag aaccaacata taataccgtt ccaacaggag gcgtaattaa accgatagat
     2821 aggttaatta ccatcataat accaaagtaa gcaatatcga tgttatacgc tcttagtaat
     2881 ggtaatagta ctggtacaaa aattaatact gctgggatta agtccattac acagccgact
     2941 aataataaga aaatcataat gacgaacatt agtacagtcg ggctatcggt aatacctttg
     3001 ataaactcaa ctaactccat aggtatttga gcaacagtaa ctgcaaatgc agatatcatt
     3061 gccgccgctg caacaaacat aaccattgca gttgttttga tagtgcctaa gaaaacatct
     3121 tggattttat ctaaggttaa cccttttaag ataatgctga ttattgctgc ataaattgct
     3181 gctacaactc ccgcttctgt tggtgtaaag atgccgcctc ttaaacctac gataataata
     3241 attggaagta gcaatggtaa gaatgctgat ttaaatgcag cccaacgttc tttacctgtt
     3301 tttttcggtt caacggtttg ttcttcgttt cggtataaga atttccaaac taaccataaa
     3361 cctaaaacca ttaaaaagcc tggaaccatt cctcccataa ataatttggt aattgatgca
     3421 ccggctgtaa taccatagac aatcattgga atgcttggtg ggataactac agaaataata
     3481 cctgctgcac agattaagcc tgttgaacgt gcaacatcat attttcttgc aaccatcatc
     3541 ggaataagaa ttgcaccgag agccgcggta tcagcaacag cggagccgga taaacctgcg
     3601 aaaattaagc ctgaaataat cactacatag cccaatccgc ctttaatatg gccgaccatg
     3661 ctcattgcaa aattgataat acgttcggaa ataccgccgt gtttcataat ttcaccggtc
     3721 agcataaaaa acgggatagc catcagcgga aaattatttg tacccattac gaaattctcc
     3781 gtgattaatt gggcatcaaa catatccatt ttccatagca taaaactggc actaagtagc
     3841 atagagagag caacgggcac tcctaaaaag agaaagagga atagacttcc taaaaatagc
     3901 cataacatat ttatttctcc tctttaatga aagctagccg aatatgtttc cataaatttg
     3961 tgaatacaat agcgaaatag gagatagatg aaataactgc tgcaaaatat agaaacgcag
     4021 atgaaatttc agttgctggc attttttgtg tgtatgtcaa taacattaac tggtatgcac
     4081 cgtaagtaat aaatgacagt gcaactaaga ctaaaatatc agcaaagaac agtaagtaca
     4141 tttgtacttt tgccggtagt gcagaaacaa caatatcaac ggtgaggtgt cccctttcct
     4201 ttgcaacgag gattattcct agaaagatca tatagacaaa gcagattctg gcgaattcct
     4261 cagaccaagc tattccggag tcaaaaaaat atcgtaaaac cgcattaaaa aagactagga
     4321 taatcattgt agatagagca attatcgcaa ggagtttgag agttctatct aatgctgtag
     4381 aaatttgcat aaatttctcc ttcttcattt agatgggcga cctcttttat ccggtcgcct
     4441 cgcttattgc ttatttggct gcgtggattt ttgaaataac atctttagcc caatcatatt
     4501 tctgatagaa attttgataa agcgtttcgg atgcttttaa catatcagct ttaaattgtt
     4561 cactcggaac aatgacttct ttgccatttt tgtggaagaa atcttttaca tcttgttcca
     4621 tcttcatcgc taattcccac tctttttctt ggtagagttt tgctgcctct aagaagattg
     4681 tctgatcttc tttagacatt ttattccaag ttttcaaact aagttgtagt aagccaggtg
     4741 agaaaatatg attagattca ataatatatt tttgcacctc gtataaacca gaatctttaa
     4801 ttaacatata tggattttct tgcccatcaa tgactttttg ttctagtgcg gtaaatactt
     4861 cacttaaagg catactttga gggttaatat gcattgcttt tgcccagtcc acaaacaacg
     4921 aatttagtgg aacccttaat cgcaaacctt taaatccatc aaaattctca acttttttat
     4981 tggaagagat aacacggaag ccattcatac catacgctag taatttaatg ccgtacttct
     5041 ccattttttc cccgatttct tgagcaatag gagattctag aactttctta gcttcggcat
     5101 tatctttaaa tagatatggc cactcccata cgccaaaaat tggatcaaca ttttgtagta
     5161 ataaacctgt aatgccacct tcaatcgtgc cattacgcaa accatttaca atttgatctt
     5221 ctcctcccaa ctggttattt gggaaaatac ggatttcgta gcgtccttct gttttttctt
     5281 caatggtttt tttgaagtat tcctgcaatg cgatatttga tggatgaaca tctgaattat
     5341 agtgaccaat cttaatgata gtcttcgcaa atgccgaacc ggtgaacatc aggctagaaa
     5401 ttaatagagc tttagtgaaa gttttgagtt tcatagttat tacctcgtag gtgagttagt
     5461 atttaaaata cagcgatgat ttttaagtaa tcatcaggat tagctgagag ttcttcgata
     5521 tagtgaggta tttcatcaaa tgtaattacc ttactaatga tatcttttgg ttcaattctt
     5581 cttgatgcca gaagctctaa tgcttcttca aatgccccct tgcttgttct tgccccataa
     5641 atattaagtt ctttgaaggt gattaaatta gttggtaatg gtgtttctgt tttcggccat
     5701 ccggttaaag caatacgtcc agcaaaagag gtgtatttaa gcgtattttg tactgcgaca
     5761 tttgctcctg acgcttcaat aaccacctct gccattcttc cattggtaat tgatttaata
     5821 gcttcaagat cgttttcttt tgttggatta attgtgtgtg gaataccaat agattttgcg
     5881 taatccaatc gcttatctaa aatgtcaatt aaaatcggcg tcgctccata agctttggca
     5941 accaatgcag ccattagccc aattgcccct gctccaatga tagcaacatg ttcaccagct
     6001 ttgacttttg ttctatgaat tgcgtgtaag gaaatagtta agggttctgc taacgctaat
     6061 tcgtgtattg gcatatcagg tgttttgatt atcatatggg ctggatgtct aacaacctct
     6121 tgcattgccc catcaatatg tacccccaat acttttagag attcgcaaca atttgttcta
     6181 ccaatggagc agggatagca atgtccacaa tagatgtaag gttcaatgat gacacgatct
     6241 cctacattaa tattatttgg cataccttta ccagactcaa ttactgtgcc aacgatttca
     6301 tgccctaaaa ttctaggata agtaacaagt ggattagtgc ctctaaatgc tccaatatca
     6361 gagccacata tccccatacc ttctactttg attagcacat cattttcacc tttaacagga
     6421 taagaaacgg tttgtatacc aactttatta ggttctacaa gacatactga tttgagtggt
     6481 ttttccatga ttttgtcctt ttttgataaa gttaatcgac attttgtagt gtcaatggta
     6541 ttttatgcac agatactaac accaaaaaac aagtttggca ttccaatttt tgtgattttg
     6601 tgaacctgat cacaaaaatg aaattttgga attccaattt cgtgaggatt tatgcaaaat
     6661 agttccagaa cttacactaa aataggaaaa attctcaaag aggaaattgc tcaaggaaaa
     6721 tacagagtag gagataagtt acctactgag agagatattt ctgaaagatt tgaagtgagt
     6781 agaactatcg taagggaggc gatagtgatg ctagaagtag aaaagcttat tgaggtaaaa
     6841 aaaggatcgg gcgtgtatgt cattagagtc ccggagcttt ttccagaaga ttttccagaa
     6901 gagaaagata atgagctaag ggatgttggg ccatttgaac tacttcaagc acgacaatta
     6961 ttagaaagca gtattgctga gtttgcagca cagcaggcta ccaaacaaga tatacaagta
     7021 cttcgtaaca tattagaaag tgagaagaaa cttttgttag agaagatgga tgactacagt
     7081 gcagataagg aatttcatct tgcattagct catatgacgg gtaacgatgt tctgattcaa
     7141 ttacaagaac agctttggcg ataccgcttt aatagtgcga tgtgggctca gctacactcc
     7201 agaattttac agcatgatca tcaccacttg tggatagagg atcatgagcg aattttaaat
     7261 gcaatacaga gaaaaaatcc tgcattagct cgtaaagcaa tgtggcaaca tctagaaaat
     7321 gttaaaacta aattgcttga attgtctgat gtagaggatc ctaaatttga tggatatcta
     7381 ttcaatgtaa accccgttgt agtaggtatc taaaacctaa actacacaat gaggagttta
     7441 ctatggaaca agcatggcgt tggtatggac caaacgatcc ggtatcttta gatgatgtaa
     7501 gacaagcagg ggcaacgggg attgtgacag cgttgcacca tattcccaac gggcaggtgt
     7561 ggtcggttga ggaaattaat aaacgtaagg cattattagc tgaaaaaggg ctggtttggt
     7621 cggtggtaga aagtgtgcct gttcacgaag aaatcaaaac tcaaacaggc aactatcagc
     7681 aatggattga taactataag caaaccttgc gtaatttggc aagctgtggg attgatacgg
     7741 tgtgctataa ctttatgccg gtgctagatt ggactcgtac cgatctggag tatgaaatgc
     7801 ctgatggctc aaaagcattg cgttttgacc aagtggcgtt tgcggcgttt gagttatata
     7861 tcttaaaacg cccgggagcg gaagccactt actcatcagc agaacaagcc gaagccaaag
     7921 cctattttga gcagatgagt gacgctgata ttgcaaaatt aaccaaaaat atcattgccg
     7981 gtttgccggg gtctgaggaa ggctatacgc tcgaagaatt ccaagcacag cttgaccgct
     8041 atcaagggct ctcggctgaa aaattccgta cccatttagc ctatttctta aatgagattg
     8101 tgccggttgc acaagaggtg ggcattcgta tggcgattca ccctgacgat ccgcctcgcc
     8161 cgattttagg cttgccacgc attgtttcaa ccatagaaga tatgcaatgg tttgtggaaa
     8221 ctcaaccgct tgcagccaat ggcttcacgt tctgtaccgg ttcatacggc gtattagccg
     8281 agaatgattt ggtcaaaatg gcagagcagt ttgcagatcg catttatttt gctcacttac
     8341 gagctaccca acgtgaagaa aatccacaaa gtttccacga agcggatcat cttgccggtg
     8401 atgtcgatat gtttggcgtg gtgaaagcat tacttaccga agagtatcgc cgcaaggcaa
     8461 atggcgatca ccgattaatt ccaatgcgtc cggatcacgg ccatcaaatg ctcgatgatc
     8521 tgaagaaaaa aaccaaccca ggctattctg cgattggtcg tttaagagga ttagcggagt
     8581 tccgtggatt ggagttggca cttaagaaag tctattttaa cgattagcct tatttattat
     8641 aatgccctct actgtagagg gcattttata gtttgatatg ttcaaaaaat atttcggcga
     8701 aataatcctt tttctcgtta cctttattgc tgcagcgggg tggtttttct ctaaacacgc
     8761 tttagttgga ttaccgccta ttggctttat gggagggcgt tttatattag cctctttgat
     8821 tcttctgcct ttttcttacc gagagctgta tcgtttagat aaggttcaag ttaaatattc
     8881 ggtgattacc ggtcttactt acggaattta tactattttt tgggtattgg ggctggcttt
     8941 tagtacagtg tttggtgaag gggctttttt ggtgagcctt gcgatgctga ttgctccttt
     9001 gctctcttgg ttgatattca aacatacgcc accgaagatt ttttggttag ccttacccat
     9061 tgcattaagt gggctttact ttctttctgc ggagaaaggc atctttcatt tttccttagg
     9121 cagtacgatt tttctggtgt catcagtgtt tggggcgtta tattttgtgt taaataatca
     9181 atacgccaaa caagtctcat tgtttccatt aattactatc caattggcga tagtcggtgc
     9241 ggtctgtagc ttgtattcct attttatgga aagttggcat ttcccgattc tcaccgaagt
     9301 gtggctctgg tttgccgcaa gtgtattgat tgcgacaaat ttacgttttt taatgcaaac
     9361 actggggcaa aaatattgcc atattggtac ttctgcactc attatgttac ttgagccggt
     9421 ttggacattg ttattgagcg tgatgttatt gggggaacaa cttagtgcga caaaatggct
     9481 aggttgcagc ttgattttga tagcattgat tatttatcga ctccctattc tacgactcaa
     9541 aaaataggta aaacaaaagc aaatatgcca tagtattgga agcagttagg cattttagag
     9601 taagtaacaa gcggtcattt ttgcaaaaaa tcttgcaaaa atgaccgctt tctttctttt
     9661 atccgttcaa tctatgttaa aattctacat ttttataaga tgagagagag aaacgtggaa
     9721 cagaatttag tcgaattttt aaccaaaacc ttagatgatt taaaggcaca agatatttta
     9781 gccattgatg tgaaagggaa atctagcatt actgatacga tgattttagc caccggtaca
     9841 tcgagccgtc acgttgcttc aacggcagag cgtttaatta cggaagcaaa acaggctggg
     9901 ttcgaggtat ttggagatga aggtaaagca gttgccgatt ggatcgtggt cgattttggg
     9961 aaggcaatgg ttcacttgtt gcaatccgat agccgtgaga tgtaccaatt agagaaactt
    10021 tgggcgtagc agttcaataa ggaacgacaa gcggtgattt ttgcaacatt ttttgcaaat
    10081 taaaccgctt gtaagtgaaa gagagatgaa aattcaatta attgcggttg gcactaaaat
    10141 gccggcgtgg gtaacgacag ggtttgaaga gtatcaacgc cgttttccga aagagatgcc
    10201 gtttgagttg gtggaaattc cggcaggaaa acggggcaag aatgctgata ctaaacgcat
    10261 tcttgagctt gaaggcaaag caatgttggc ggcagcgggc aaggcaaaaa tcgtgacttt
    10321 ggatattccg ggcaaagcgt ggacaaccga gcagttagcc actcagttag agggctggaa
    10381 aaacgatggg cgagatgtag cgttattgat tggcggcccg gaaggattat caccggaatg
    10441 caaagccgct gccgagcaga gttggtcgtt gtcgccttta accttacctc acccactggt
    10501 gcgggtggtg gtggcggaaa gtttgtatcg ggcgtggtcg gttaccatta atcaccctta
    10561 tcatcgagag tagtgagtca ttttagatag tgttatatgt ttggtaaatt agcaaaattc
    10621 acagcttcaa accagcgaga aaaggtgcga gacgggcagg cagaaagcaa tctgtatgcc
    10681 cgccgtgcct tagttgcgtt tctgggggtg ttggcgttaa gtggtgtttt attaaccaac
    10741 ttgtatcact tgcaagtggt gaattatgag gactaccaaa cccgttcaaa cggtaaccgc
    10801 attaaattgt tgcctgtgcc accggctcga ggcttaattt atgaccgtaa cggtaaagta
    10861 ttggcggaaa atttaaccta tttcgggctg tttattgtgc ctgaaaagac ggaaaacatt
    10921 gagcagactt taaccgagtt gaaggatatt gttgggctga cggacgaaga tatcgagaat
    10981 tatcataaag agaaaaaacg cagttctcgc tataccccaa ttatgctcaa aggcaatttg
    11041 aatgaggctc agatggctcg ttttgcggtg aaccaatacc gttttccaag tttagatgtg
    11101 caggcgtatt ataagcggaa ttatccttac ggcgagcttt taacccatat tatcggttat
    11161 gtggcgaaaa ttaacgaaaa cgataagaaa aagctgcaag aagccgggaa attcggcaat
    11221 tatgccggtt cgcacgatat ggggaaattg gggattgaaa aattctatga agatcagctc
    11281 cacggtgtca ccggttttga agaggtagaa attaataacc gagggaaagt aatccgtaaa
    11341 ttgcgtgacc aagcaggggt tgccggtagc agtattcgct tgagtattga tgtggaatta
    11401 cagcaatatg tgatgtcgtt aattgctaag cataaagggg cggtggtggt gcttgaccct
    11461 cgtgatagca gtattttagc gatggtctca aacccaagtt atgacaataa tttatttgtg
    11521 ggcggtattt cagggcagga ttataaagca ttattggacg atccaacccg accactttat
    11581 agccgtacta cacaaggcac ttatccaccg gcttcaacta ttaagccatt tatggcggtg
    11641 gcaggtttgg aagagggaat gattacccct tctaccacca tttcagaccc cggctattgg
    11701 attttgcccg gcactacaca acgttatcga gattggaaac gtagcggaca cggtgcaact
    11761 aatgtgaata aagcaattac cgaatcgtct gatacctttt tctatcaatt agcctataaa
    11821 atggggattg ataaaatgtc ggattggatg aaaaaattcg gtttcggagt gaaaaccggt
    11881 atcgatattt ttgaggaatc cgcaggggta atgccaaccc gtgaatggaa acaaaaacgc
    11941 tataaaaaac cttgggtgca gggcgatact attccggttg ggattgggca gggctactgg
    12001 atttcgaccc cgttacagtt ggcgaaagcc acggcggtgt taatcaacaa cgggcgggtg
    12061 aatacaccgc atttaatgct tgaagtgcaa agcagtaatt cggttacgcc gtatcaagac
    12121 ccattgttgt atgaagatat taaaggcgtg ccggatgtct attggaattt agcaaaacag
    12181 ggaatgtata acgtaaacca cgccgcaaac ggtacggcac gaaaagtgtt tgccggtgca
    12241 ttttatcatt cagcagggaa aaccggtacg gctcaggtgt ttaacttaaa cggtaaaaat
    12301 tacgataaaa atgcgattaa aaaagagttg cacgaccacg cttggtttat cggctatgct
    12361 ccttatgaac aacctaaaat agtggtggca ttaattttag aaaatgcagg cggtggtggt
    12421 agtgccgcgg ctcctgttgc tcgtcagatt atggattatg ctttgagaaa aggcattggt
    12481 gaacagccgg attcgcaaaa ttctgagcaa aataaaccgc ttgtcacaga ggaacatcat
    12541 gaatagtggc tttaaaaaac ttttctggaa aattttttca ctcgatctcc ttttattatc
    12601 aggattatta gctattaccg gttatggttt attagtacta tatagcgcaa gcggtgcaaa
    12661 tgagcggatg tttaccagta gactgattca agtttcgcta gggcttgggc taatgttgat
    12721 aatggcaatg gtacctcctc gcttttatca aaaagtttcg ccttatctgt atattttctg
    12781 tctgatatta ttgattttgg tggatttggt cggcgaaacc agtaaaggag cgcaacgttg
    12841 gttgaattta ggatttgttc gctttcagcc ttctgagatt gccaagctcg ctgtgccact
    12901 gatggtagcc acttatttag gtaagcggcc gcttcctccg agttttaaag atacttttat
    12961 cgccttagcc attattattg tgccaacctt attggtggca attcagcccg atttgggaac
    13021 atctatttta gtgtgttctg ccggtatttt tgttttattt ttggcagggc taagttggaa
    13081 attaattggc gcaggtgtgg tgtttttagc cggttttatc ccgataatgt ggtttttctt
    13141 aatgcacgat tatcaaaaaa cccgggtgat gaccttaatt gatcctaata aagacccgtt
    13201 gggtgccggc tatcatatta ttcaatccaa aattgcgata ggctctggtg gcattaatgg
    13261 aaaaggctgg atggaaggaa cgcaatccca gcttgaattt ttacctgaac cgcataccga
    13321 ttttattttt gcagtgttaa gcgaagagca tggtatgata ggtgtgctga ttttattagc
    13381 gatttatttg tttattattg cccgaggttt agtgattggc acaaaatcgg attctgcttt
    13441 cgggcgtata ttatcaggtg gtacagccct tctatttttt gtttacgttt ttgtaaatat
    13501 tggtatggtg agtggtattt tacccgtagt cggtgtacca ttaccgcttt tcagttacgg
    13561 cggaacctcc tatgtgacgc tgatggcggc atttggtttg atgatgtcaa cctatgttca
    13621 ccgtaagcgt gtaggtagta ataatcctta tgataaactg aaataaatta aacttttgct
    13681 tttttgcggt atcaaaaagc agtgaaacga tcatcgttac aaacaataac gacatccaca
    13741 tagaggaaaa aacttatatg agattaggta aaattgtaac cctgttgtta gctggatttc
    13801 tgacatttag ttcattaaat tctttggctg cgacaaagaa tacaaaagct aaatcgagta
    13861 ttttagccaa gaagcaagca acttctaaag caataaaaaa agtaggtatt tctaaagtta
    13921 agaaaaaaga agtagtggct aaagctacaa aaaagaaaac aatttctact gttacaacta
    13981 agccgattaa atctacaaaa aagctggctc gcaaggttgt caagaaaacg gtaaccgcta
    14041 agaaaaaatt ggtaaaaaag cagcataagc cactggtcaa aaaacatttc cagactggtg
    14101 ttgccagcta ttatgcaaat tactttaatg gtcgccgaac cgctaatggg gagactttca
    14161 ataacgcaaa aatgacggct gcacatcgaa ctttaccatt tggtacttta attgaagtga
    14221 cgaatttacg aaatggtcgt tctgtggtgg tgcgtgtaaa tgatcgcgga ccttatgctc
    14281 acgcacgggt cttggattta tcatcagccg cagcgaaaaa aatcggtatg caccattcgg
    14341 ggacggcaag agtgaaaatt gcgattgttg ataagtcaaa attgggatcg actaatcagt
    14401 tttatgtaat gaattaattt aagagggaat atgaaaaaaa cattagtgaa agcgatgagc
    14461 gtggtaggtt ttgcggtttc aagcattgcc tatgcggatt taaacactaa cttcaattta
    14521 tcggcacccc agatcaacgc acaaacctat attttgatgg attacaactc cggtgaagtt
    14581 ttagtgagcc taaatccgga tcaacgtcaa tatcctgcat cattgaccaa aatgatgact
    14641 agctacgtag ttggtgaagc cctaaaatcg ggaaaagtgc aaaacagtga tatggtgata
    14701 gtcaccgaaa attcgtgggc gcaaaaattc cccggctctt cattaatgtt tttagattta
    14761 aacagcaaag tttcagtagg tgagttgatg aaagggctga ttatcgtctc gggtaatgat
    14821 gcctctgtgg cgcttgctga acatgtttca ggctcacaag cggcgttcat taatgaaatg
    14881 aacaaatatg cccaacagtt tggcttaaag aatactcact ttaccactgt tcacggttta
    14941 gatgaccctg agcaatattc ttctgctcgt gatatggcga ttattggttc gcatattatt
    15001 cgtgatcagc cggaagagta taaaatttat gcggaaaaag agttccaata taacatcaaa
    15061 aaaccacaac caaaccgcaa cggattattg tgggataaaa ccattcaagt tgatggtatg
    15121 aaaacagggc atactgataa agccggctat aacttagtcg catcagcaat taacccgaat
    15181 acacgtttaa tttctgtagt gatgggcgtg ccaacttata aagggcgtga agtggaaagt
    15241 aagaaattac tgcaatgggg ctttgcgaac tttgaaacca ttaaatctat tccggcagct
    15301 caatcggtgg cagagcaaag tgtctattac ggtgaagtca ataaagtaca attaggctcg
    15361 gtgcaagata gctttgtgac tatcccgaaa ggtcgctcgg cagatttaaa agcccgttat
    15421 gagttagaga agaaaaacct cgaagctcct ttagcaaaag ggcaggtagt gggtaaaatg
    15481 atctaccaac ttgacggcaa agatgttgca agcactaact tacaagtctt acaagatgta
    15541 ccggaagccg gtattttcgg caaagcgtgg gattggattg cccttactgt gaagagcttg
    15601 ttcgattaat cttgttattt gcctttttat ccccatctag ggagcaatgc aagcggtgtg
    15661 attttgcaaa aaatttgcaa aatttcaccg cttaccgttt tagatatgaa tgtataaaaa
    15721 gtgtagtggc gaaaaatttt tcgcccctcg aagaaagaat agaagaagga tcaaaaatgt
    15781 ctcaacccaa agcgataaac ttacaagatt tacctcaagc caaattaaaa gatctgctag
    15841 agtttccgtg ccatttcacg ttcaaagtgg tgggagcaaa ccgagaagat ttggttgatg
    15901 atgtggtagt ggtcacgcaa aaatatgcca aaggcgatta caatccacgt cagcaaaaaa
    15961 gctcaaaagg tacctataat tccgtttcga ttgatattgt ggcggaaaat attgagcaag
    16021 tagaaactct ctacacggaa cttgccaaaa ttaatggcgt aagaatggta ttataatgcg
    16081 aggggcgaat gacattcgcc ctcaattttt caataaagcg atgttggaaa atagaaaatg
    16141 aaattaataa ttcgccaact aggcattcag ccttatgaag aaatttggca ccaaatgcaa
    16201 tctttcacag atagccgtga tgggaatacc gatgatgaga tctggctggt tcagcacccg
    16261 gctgtgttta cgcaaggatc ggcaggcaaa ccggagcatt tgttaaatcc aaccaatatt
    16321 ccggtggtgc aaagcgaccg tggcggtcaa attacttacc acggcgaggg gcagcaaatt
    16381 atgtatgtgc tgattgatat caaacggctg aaatccttgg gtaaagaagt gtcggttcga
    16441 gatttagtga ctgccctgga gcaaaccgtg gtacaaacgt tggcagaata tggcatagaa
    16501 agttatccaa aagccgatgc acccggggtg tatgtggacg gcaagaaaat ctgctcactt
    16561 ggtttacgaa ttcgaaaagg ttgctctttc cacggtttgg cattaaatat caatatggac
    16621 ttaacgcctt ttcaaaacat caatccgtgc ggttatgcag ggctggaaat gtgccaatta
    16681 agtgaactgg ttcatcgccc tgatttgcgt tgcgaagagg ttgctccaaa attagtggct
    16741 catttcagca caattttagg ctataataac caacaaatta ttaacaatta ggataaaaca
    16801 cagtaatggc tacatcgaca gctcaaacga cggcaactgc tacagtgaaa aaaatgagcc
    16861 ctttcaaaat ggaaaaaggg gtgaaatatc gtgatgccgc caaaacctct gtgattcagg
    16921 tgcgtaatat cgacccggat caagaactct taaaaaaacc ggagtggatg aaaatcaaac
    16981 tgccggcaaa ttcggcaaaa attgatagca ttaaaaacgg tatgcgtcgc cacggcttgc
    17041 actcggtgtg cgaggaagcc tcttgcccga atttacacga atgttttaac cacggtactg
    17101 caacctttat gattatgggg gcaatctgta cccgtcgttg cccgttctgt gatgtggcac
    17161 acggcaaacc gcttccgctt gacccggacg aacctcgcaa agtggcagaa acggtgcaag
    17221 atatgaaact caaatatgtg gtgattacct cagtggatcg tgatgatttg cccgaccgtg
    17281 gtgcggctca tttctctgcg accgtgcgtg aaatcaaagc cttaaatccg cattgtaagg
    17341 ttgaaatttt agtgcctgat ttccgtggac gtgtggagca agcggtggca attctcaaag
    17401 aaaatccacc tgatgtcttt aaccacaacc tagaaaatgt gccacgtctc tatcgtgaag
    17461 ttcgtccggg agcggattac aaatggtcgt tggaattatt gaaaatcttc aagcaagagt
    17521 tcccgcatat tccgaccaaa tccggcttaa tggtaggctt aggtgaaacc aacgaagaaa
    17581 tcttggaagt aatgcaagat ttacgagacc acggcgtgac gatgctcacc atcgggcaat
    17641 acttacaacc aagccgccac cacttaaaag tggaacgtta tgtgccaccg gcagagtttg
    17701 atatgttccg tgaggaagct gaaaaaatgg gctttgaaca cgcagcctgc ggaccgttcg
    17761 tgcgttcttc ttaccacgcg gatttacaag cgaaaggcga attggtgaaa taaaatcgat
    17821 aagggcgaat attatttgcc ctttgttttt gtggtttgca aagttttagc aaaattaaac
    17881 cgcttgtaat ctcttctaat ttactcttct atagataatt tcgttgttgc gaattatttt
    17941 cattgactat ttgttataga aagttacaat tcaactttcc atttcttttt ggagttccaa
    18001 tgaaaaaatt actattaaac agcgtatttg ctgccacatt gcttgctttt ggtgcaactg
    18061 ccaatgccga aattaaaacc atcactgatg tattagggcg tgaggtcaaa gttgatgtgc
    18121 ctgctaaacg tgttgcactg actttctatt accctgacta tattgcggta accggtgttg
    18181 aaaatttcga taaggtagta gggatttcac gagagttttg ggaaaagttc aacccaggca
    18241 gttgggcatt atttgcagaa aaaatgccaa atctaaaaaa cattgccgat cttggctatg
    18301 tgaatagtgg tactttttca acagaaaaaa ccatcgcatt aaaaccagat gtgttagtgc
    18361 tacctgaaat tcaatatcaa gcgttagcca gtgaaatttc acgcattgag caagctggca
    18421 ttcctgttgt agtagatttc aacaagcaaa ctattgaaaa ccatacaaaa agcgtgagag
    18481 ttttcggtca gcttacaggc actgaagaga gagcggagaa aattgccaaa gagtatgaag
    18541 agggcattgc tgatattcag agctgtattg ataatgcaaa agtaagtaag ccgaaaatct
    18601 atgctgaatt tggtaataaa ggcccgaaag agcatagttt tacctttggt aaaaatatgt
    18661 ggggagcgat tgccgaaacg gtgcgaggcg ataacattag tgccccgttt gtagaaaact
    18721 gggggcagat taaccctgag caggtgttag tttctaaacc tgatgtgatt atgatttcag
    18781 gtacggaact aggcaacgac actaaccctg agattatgtc gatggggatt aatattgaag
    18841 aagccgatgc tcaaaaacgc ttaaaaggct ttattgagcg tacaggttgg aaagagttac
    18901 ctgcggtaaa atcagggcgt gtttatggtt tatatcatac agcttctcgc tcaatttcag
    18961 atttagcgag tgctcaattt atggcaaaag cactttatcc tgaattgttt aaagatatcg
    19021 atcctgaaaa aacgtatttg gatttccaca acaactactt accaattacg ccaaaaggca
    19081 cattctttat gcaactgaaa taaaaataac aagcggtcat tttgcagatt tattttgcaa
    19141 aatgaccgct tgtattcgct tttactattt gcggttcaaa cagcaatcaa tatttaaaca
    19201 tttgcgaagc gggcggataa tcggcgagcc gtttccatca tataaaatct caatattgac
    19261 attatagagt ctctgaataa tatcgcattc caacatatca caagctctgc cttgtgcttg
    19321 aatttcccct cgcttaatag catcacacta tccgaaaatt gagccgcaag gcttaaatcg
    19381 tgcagcacca taattgtaat taggttattc gctcgagtgt aggcattcac atattcaagt
    19441 aagttgattt gatgatacat atccaacgca ctgacaggct catctagcag cagaatgtgt
    19501 ggtttacgta gcaatacttg tgcgaacatt accatttgcc gttgcccgcc acttagccgc
    19561 ataatatctt gatgagctag atgtgaaata ccgagtttat ccataatatc aacggcttca
    19621 ttgagtaatt catcgctaat atacatattc aggctatcaa gttgccctaa taaaataacc
    19681 tctaaggcac tgagtgaggc ctctacctgt gtgtcttgtg gcatatagcc aatttcttta
    19741 cgccaactat gcaagtggct tgaggttaat ttattaccgt caaaatcaat tgtgccttga
    19801 tgagcaattt ccccaaatag agttttaagg agagaagatt tacccgcacc atttgggcca
    19861 agtaaagtat agatcttgcc ttgttcaaaa tgtagattga ttttatcagc caccact
//
LOCUS       DANLUQ010000031        19881 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.031, whole genome shotgun sequence.
ACCESSION   DANLUQ010000031 DANLUQ010000000
VERSION     DANLUQ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 19881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19881
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.031"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            238..450
                     /locus_tag="PXB27_000871"
     CDS             238..450
                     /locus_tag="PXB27_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL5345688.1"
                     /translation="MSAITGTVKWFNATKGFGFITPDQGGKDVFVHFSAIEANGGYRT
                     LEDGAKVQFEIQDSERGASAVNVKTI"
     gene            complement(510..1664)
                     /locus_tag="PXB27_000872"
     CDS             complement(510..1664)
                     /locus_tag="PXB27_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HDL5345689.1"
                     /translation="MMKVSPFQWAAFNYFGFFCAYGVLLPFLPVWLKHHGYSTEMIGL
                     FAAVGYLFRFAGSMLASQRVKTVSQLIPTARSLTWLNIAAAAALLFSGDFIWLLFPVL
                     MLFQMFNSGAMPIADSIASIWQHQIGLDYGRARLFGSMAFVVGSLSAGYLIGLLGENS
                     VVWIIIGFLVFLGIGQLATPKVGFEEQGNKANTSSVTYRQLLKEPETLKMLIAVSLIS
                     ASHAAYYTYSTIHWSNASISTAMTSLFWGLAVVAEILFFLVSKRLFKSWKISHLMILA
                     AVFAVIRWAIMGSTTEIVLIIVMQMLHSITFGAAHIAMIRYISMQSAERVTKLQGLYF
                     GLASCAFMAVFTFIAGMVYQHSPSLTFWLMAIFTLPAIFIVPKRFEVQIN"
     gene            complement(1661..2479)
                     /locus_tag="PXB27_000873"
     CDS             complement(1661..2479)
                     /locus_tag="PXB27_000873"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDL5345690.1"
                     /translation="MNEKKLAFIGTGNMAYAIIQGLLKSGYSAEQIIACNKSNLARRE
                     ELQAVGIATNFANREAVEQADVIVLAVKPQMMAQVCAEFADVDFSNKWVISVAAGISV
                     QRLETLLPSAKNIIRTMPNTPSLISEGMTGLFAKKSVNPTACQFAEQLMSAVGKCYWV
                     ETEEQINHIIAITGSSPAYFFRFMEAMQQSAVQMGFSEQDARTLVQSVALGAAKLVEA
                     NPTTPLSTLRENVTSKGGTTAKALEVFEQHDLAKTVDDAMWAAIHRAREMEQSL"
     gene            2556..3464
                     /gene="rdgC"
                     /locus_tag="PXB27_000874"
     CDS             2556..3464
                     /gene="rdgC"
                     /locus_tag="PXB27_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HDL5345691.1"
                     /translation="MVWFKNVMIYKLTGELALESNSLNELQQETRFTPCSQHDMSKFG
                     WATPLTGSDLLHFSQANQFLLISHKEDKLLPANVVKAETEQRIAELEEKEARKLKKTE
                     KQAVKDQVVSALLPRAFSKHQFTALWLDLDNQLVYVDAASSKRAEDTLALLRKTLGSL
                     PVVPISFTLPPAEVMTNWIAKGHTPSWLNLLEEAELKSFDTDSIIRCKRQDLETEEIA
                     RHLQAGKFITKLAIEWEGHFNCVLNEDATLSRLKFADEVREKNDDILKEDVAQRFDAD
                     FLLMTEELKLFTEKMIAEFGGIKSRI"
     gene            3485..4090
                     /locus_tag="PXB27_000875"
     CDS             3485..4090
                     /locus_tag="PXB27_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase family hydrolase"
                     /protein_id="HDL5345692.1"
                     /translation="MLDQTIIDQYDALIFDMDGTLIDTMPSHAKAWEKVGEVLGYPIN
                     PKPMYELSGSTTFVIAREIMQRSNIPEHYFEQVVQLKREFGIEMVLANATLLPAFEII
                     KANVGKKPMAIGTGSHRAMVELLDQKFNLRQYVSVIVDSDDVSNHKPAPETFLKCAEK
                     LGIAPHRCLVFEDADLGVQAALNGNMNVFDVRTNTITHSTR"
     gene            4106..4897
                     /locus_tag="PXB27_000876"
     CDS             4106..4897
                     /locus_tag="PXB27_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDL5345693.1"
                     /translation="MKTEWIKHLIQQLTTLSKAIENYFTSGSFGVKFYPFLMHPCFRK
                     SCAFLIFIIAMVSSFSGLFYLVAEHITILFSERSLTTYFHHSTLELMIPVGTMIDEKI
                     SELSPLSVVMRSMFVLQAVVFFFIYAIGITHIAHNKLRVIGLLLSLCFAIGCSLVAGI
                     QPSSRGEFGIYNLGASITFLIGNLTLIIAGFDLYHPSMQFFKRFSIGAGVIGAICILI
                     TIFLPTLFSPLIERAGIYTIMLWEILAGFAIIKRLLKLPSFRQQG"
     gene            4901..6043
                     /gene="argE"
                     /locus_tag="PXB27_000877"
     CDS             4901..6043
                     /gene="argE"
                     /locus_tag="PXB27_000877"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016533842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HDL5345694.1"
                     /translation="MKQIPFIQRYAQLIELPTISSLVAEEDLSNRRLIELLATWLADF
                     GFKTEILAVEGSRNKYNLLATYGEGEGGLLLAGHTDTVPFDEGKWRFNPFQLTEKAGK
                     LYGLGTADMKGFFAFVVEVVSQLDLTQIKKPIRILATADEETTMLGARTFAQHSHIRP
                     DCAIIGEPTSLKPIRAHKGHIGESVRITGRSGHSSDPAKGINAIELMHQSIGYLMKMR
                     DELREKYHNPLFQVTHPTMNFGNIHGGDAINRICACCELQFDMRPLPNMAVQDLYAMV
                     NEHLKPMLEQYGDLIEIRHLHDGIPGYECEHSAQIVQVVEKLLGEKCDAVNYCTEAPF
                     IQQLCPTLVLGPGSIEQAHQPDEFLEMKYIEPTKELLTKLIYHFCG"
     gene            complement(6190..6477)
                     /locus_tag="PXB27_000878"
     CDS             complement(6190..6477)
                     /locus_tag="PXB27_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5345695.1"
                     /translation="MPKSWELSLTARSNVIEILQSVEDYTGHWLSAVSLWEQLQNKLD
                     LIAFMPTIGRRREDGTLETFCRNYRIVYEIHLEKVYIITIIHSRRLYPRPS"
     gene            complement(6470..6661)
                     /locus_tag="PXB27_000879"
     CDS             complement(6470..6661)
                     /locus_tag="PXB27_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345696.1"
                     /translation="MLFKEQGYDEFLAEQIKAGQEAIARGETFTKEQINNELNHLFAD
                     MQKRQQLRENENLFEVNYA"
     gene            complement(6951..7541)
                     /gene="yraP"
                     /locus_tag="PXB27_000880"
     CDS             complement(6951..7541)
                     /gene="yraP"
                     /locus_tag="PXB27_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HDL5345697.1"
                     /translation="MNKLANKLLKGSLLVLGFISLQGCITTAVVTSAAVATKVATDPR
                     TTGTQVDDEILEEKVAYNINKDEQIKQEARINVVAYNGKVLLIGQAPSMDVVENAKNL
                     AAGAEGVTEIYNEIRQGEKIGFGQITQDSWITTQVKSKLLVNSEVKATEVKVVTENGE
                     VFLMGKVSQNQADAAAEAARNVGGVTKVVKVFSYAQ"
     gene            complement(7551..8135)
                     /locus_tag="PXB27_000881"
     CDS             complement(7551..8135)
                     /locus_tag="PXB27_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL5345698.1"
                     /translation="MLNKIQDRFTESIQTQISASELLPAKLSEAANRIVACLLRGDKV
                     IVCGHGRSYGNAELLVSHLLHRYELARPSFAAVQLQLSGVLAGVVAQDNELEAVYKKQ
                     LQAVAKEGDLFIVFSPVGNEECILQAIHTAKNQHLDLDIIALTGSNNDHTLGLLDESD
                     IEISAPSTNQLRVIESHHFYVNLLCELIDHLLFS"
     gene            complement(8141..8512)
                     /locus_tag="PXB27_000882"
     CDS             complement(8141..8512)
                     /locus_tag="PXB27_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HDL5345699.1"
                     /translation="MELFNKLINKREQGSFYEQKARVFLEQQGLRFVAANQAFKGGEL
                     DPIMQDGQTVVFVEVRQRKSNRFGSAVESIDYRKQQKWQNAANMWLLKQNQSLETANC
                     RFDVVAFEGNQTPVWIPNFLG"
     gene            complement(8487..10208)
                     /locus_tag="PXB27_000883"
     CDS             complement(8487..10208)
                     /locus_tag="PXB27_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HDL5345700.1"
                     /translation="MATILNHTMKKALVPTAIALFISACTSVNPVTESIKNEAYSSSE
                     FYINKADQTKEAEDKISYQLLAVRKLIDENKEYEAQNTFSEILTAEMNEVQKLEYALV
                     SAQLAALQGKNEQATSQLKAIQDPLLSSAQRLRYIQTQARIAANQKDVIGIVRARSQL
                     NNFYKMNRERQENNDIIWQTLRDANRGMLEKTVPEAGEMELAGWLALINIYNQNVTTP
                     AQMPQAINNWKAQYPSHSAVAVMPTELQGVSNFQQTQLNSVALLLPLSGDAKILGDII
                     KRGFNDAKADDSTAVQTFDTDSSDVNSLISQAKQQGANVIVGPLLKSRVDEMLLSPEI
                     QNVNILALNATENVRNIAQVCYYGLSPESEAQSGAEKIYQDGNSVAIVAAPQDDYGNR
                     SAEAFAKRWRQLTNSDADVRYYNQPLDAVAAIQNAGVSKAALYILGNAEQVLEIKQGI
                     DSSTLKDRLAIYTSSRSNSPNNGIDFYTSMEGVKFSEVPLLADQSSSEYKKAENLANS
                     DFSMMRLYAMGADAWSLATKFNEFRHIPGYKISGLTGELKAGQHCNIERSLSWMQYRN
                     GAIQQAY"
     gene            10282..11154
                     /gene="rsmI"
                     /locus_tag="PXB27_000884"
     CDS             10282..11154
                     /gene="rsmI"
                     /locus_tag="PXB27_000884"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDL5345701.1"
                     /translation="MKNRQNSSGINPNYGILYIVATPIGNLGDITQRALDTLSSVDLV
                     AAEDTRHSGLLLSHYGIKKPFFALHDHNEQQKAVVLVEKLAKGENIALISDAGTPLIS
                     DPGFHLVRHCRQAGVKVVPIPGACAAVTALCASGIASDRFCFEGFLPAKTKSRCDKLT
                     ELENEPRTLIFYESTHRILDTLADMQTVFGEDRYVVMAREITKTWETIYGDQLGNLIE
                     WLNEDSNRIKGEIVLVVEGKPQSSEEAFSSQAIKLLTLLCQELPLKKAAAIVAETFGY
                     KKNALYQYGLEHFE"
     gene            complement(11399..12565)
                     /locus_tag="PXB27_000885"
     CDS             complement(11399..12565)
                     /locus_tag="PXB27_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5345702.1"
                     /translation="MTNLLLAIIYLSFISLGLPDAILGSAWPSMHQQLAVPLSYAGVV
                     SMIMSIGTITSSLMSDKLTYHLGTGKVMVISVALTALALWGFSISDAFWILCLWAIPY
                     GLGAGAVDAALNNYVAVHYASRHMSWLHCMWGVGASIGPHILGYALLQGRGWEAGYFT
                     ISMLQIGLTLMLFCSLPFWKLRKQITAEINHSAKPMSLKETLKIPGVKYGILAFFGYC
                     GVEQTAGLWASSYFVMVKDVGLEQAAAFGSIFFIGITVGRGVSGFLTLKFSNRQMIKL
                     GSALILVGIAILCLPLGKYVTLASLIIIGLGCAPIFPCLIHSTPAFFGEDKSQSVIGV
                     QMASAYIGVLLLPPIFGFIANAISIQLLPLYLLLMFAIMAYAYRNLVRETSPSP"
     gene            complement(12717..14231)
                     /gene="putP"
                     /locus_tag="PXB27_000886"
     CDS             complement(12717..14231)
                     /gene="putP"
                     /locus_tag="PXB27_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HDL5345703.1"
                     /translation="MFGLDPTTIMFVVYILGMIAIGFIAYRVTNNLSDYILGGRRLGS
                     FVTALSAGASDMSGWLLMGLPGAVYAGGLIEGWIAIGLTIGAYLNWKLVAGRLRSHTE
                     HSGDALTLPEYFHNRFGDKTRLIKILSAVIFLLFFAIYCASGVVAGARVFENLFGLPY
                     HQAIWYGAVATILYTFIGGFLAVSWTDTIQASLMVFALILTPFFVMYNLGGFDEMQAL
                     IDRAGEMAQKDYNDIFTGTSVLGIISLMAWGLGYFGQPHIVVRFMAAESVKSLENARR
                     ISMTWMIICLLGAIGIGYFGMAYFFGNPEQVGNISKNPEQIFIELAKLLFNPWVAGIL
                     LSAILAAVMSTLSAQLLICSSAITEDLYKGILRPQASDKELVWLSRLMVLAVAALAIY
                     IAQDPDSKVFGLVRNAWAGFGSAFGPVVLLSLFWKRMNGFGAIAGMLTGALIVHFWGD
                     ITLKLALPEIYSMIPGFIAATLMIIVVSLITPMPSKAVLDTFDRANKAYLAEVK"
     gene            complement(14325..15113)
                     /locus_tag="PXB27_000887"
     CDS             complement(14325..15113)
                     /locus_tag="PXB27_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDL5345704.1"
                     /translation="MNAKKEKESSGNQSLIRGLTLLELLAKFPNGCPLAHLAELSGLN
                     KSTVHRLLQGLQQEGYVRPAPTAGSYRLTTKCLAIGQRALSSINILHIAAPHLEALNL
                     TLGETVNFAMRENDHAILINKLEATTGMMRTRAYIGQQMQLYCSGMGKLFLAYGSADY
                     IPQYWQTKSDIIQKLTVNTITTIDGMQTELETIRRQGFAMDNEENELGVSCIACPIFD
                     HQQKVHYAISVSLSTARLNQLGRENLLPAIQETAEKISAELGGR"
     gene            complement(15110..15688)
                     /locus_tag="PXB27_000888"
     CDS             complement(15110..15688)
                     /locus_tag="PXB27_000888"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDL5345705.1"
                     /translation="MNQSSLTDLRSQLRQSMRLKRQSLTAGQQTQAAESIIPQALSLI
                     ESYQASHIAFYLPFNGEISPIPLMEQLLQQGKKLYLPLLHPFTSGQLLFVNYNCKTIL
                     KFNRLGIQEPILDVRNILPLQELEMIFTPLVACDKAGNRLGMGGGFYDRTLAQAPHLI
                     SVGLAHECQQVEQLPIESWDMPLDHIILGAAK"
     gene            complement(15690..15881)
                     /gene="ssrS"
                     /locus_tag="PXB27_000889"
     ncRNA           complement(15690..15881)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PXB27_000889"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(15986..16276)
                     /locus_tag="PXB27_000890"
     CDS             complement(15986..16276)
                     /locus_tag="PXB27_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDL5345706.1"
                     /translation="MSANNIELQIFGQVLRLQCPVEQQEHLLASAQRLEERVVVLKEQ
                     SGIIQLEKVLAIVALNLNYELEQAQRQNAENKNVLVACIEQLDNSLGKFQSN"
     gene            16355..16912
                     /locus_tag="PXB27_000891"
     CDS             16355..16912
                     /locus_tag="PXB27_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HDL5345707.1"
                     /translation="MAITDSQQFKQLVSQLQIDDTPAEFHGFLCGLIAGGIQDESWKT
                     LTYQFTNDGHAFSIEPLNTLTDFYQQLSESFSEANTLFSLWLPQNEEDGFALADGISE
                     WTNSFLLGLGVAQPKLQQETDEVGEAIDDLDEIAKLGYSDDDNNEELLEAGEEVLEYL
                     RVLALFLHSHFALGVPKTEEKPPLH"
     gene            17010..18074
                     /gene="hemE"
                     /locus_tag="PXB27_000892"
     CDS             17010..18074
                     /gene="hemE"
                     /locus_tag="PXB27_000892"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HDL5345708.1"
                     /translation="MSELKNDRYLKALLREPVDMTPVWMMRQAGRYLPEYKATRAEAG
                     DFMSLCRNADLACEVTLQPLRRYELDAAILFSDILTVPDAMGLGLSFGAGEGPKFARP
                     VASLNDVNQLPIPDPEDELQYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTKIKKMLYAEPHILHKLLDKLADSVILYLNAQIKAGAQAVMVFDTWGGVLAHED
                     YKAFSLHYMHKIVDGLIRENEGRKVPVTLFTKGGGLWLEAIAETGCDAVGLDWTVDIA
                     DARRRIGDKVALQGNMDPSVLYASPERIEQEVKNILAGFGNGSGHVFNLGHGIHLDVP
                     VESPKVFVDAIHNYSKQYHK"
     gene            18221..18811
                     /locus_tag="PXB27_000893"
     CDS             18221..18811
                     /locus_tag="PXB27_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF416 family protein"
                     /protein_id="HDL5345709.1"
                     /translation="MRNPIHKRMERFEPWQNTTFMACLCERMYPNYQLFCEVTEQPEK
                     AKVFQNILNLVWEYLTIKGAKINFDNQLEKLEEIIPDVNEYAFFGVLPAQEACEALSE
                     LLHSIIAGSTLEQAIRISQISLGTVASYLEMQQDKELNEQELKNSEEIQEELDVQWQI
                     YRLLNECEKRDLDLIFELKDEIRSSGISNIGLNINQ"
     gene            18947..19219
                     /locus_tag="PXB27_000894"
     CDS             18947..19219
                     /locus_tag="PXB27_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005819506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDL5345710.1"
                     /translation="MNKTELIDAIAAGANLTKKDAKAALEATLDAISASLKAGDAVQL
                     IGFGTFKVNERKARTGRNPKTGEEIKIAAAKVPAFVSGKALKDLVK"
     gene            complement(19308..19868)
                     /locus_tag="PXB27_000895"
     CDS             complement(19308..19868)
                     /locus_tag="PXB27_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345711.1"
                     /translation="MKKSFLSLLVLGTTMALAACDQAKEAAQSTTDKAVEVKEAVAEK
                     AAEVKSATEAKVEELKAAANDAAQATADKTEEAKKAVAEKATELKTTTEAKVEELKAA
                     ANDAAQATTDKTEEAKKAVAEKAAELKATTEAKVEELKAAASDATQATADKAEEAKKA
                     VAEKAAEVKVETETKVEEMKKAIESK"
BASE COUNT         5821 a         4411 c         4049 g         5600 t
ORIGIN      
        1 attccacatt acgtttacaa aagagataga ctcttttttc atttgaatgt tcataaaagt
       61 ataaaaatga tttgcttaaa aaatgaataa gtggtgtaat ggctcaagct gttcgtgcgt
      121 taattaaagt tgaatcttgt tagttatttt cctcaaggca agtgattgat agatacgtct
      181 gtaatatccc gtttagctat cttttatcac tttttgtatt tatataagga aaattttatg
      241 tcagcaatta ccggcacagt aaaatggttc aacgccacca aaggttttgg ttttatcacc
      301 cctgatcaag gtggcaaaga tgtattcgta cacttctctg ctatcgaagc aaacggcggc
      361 taccgcacat tagaagacgg tgctaaagta caatttgaaa tccaagattc agagcgtgga
      421 gcttcagccg taaacgtgaa aacaatctaa tttcagcaaa ttcaatgaat ttcgataaaa
      481 atgccgacaa cttgtcggca tttttgctat caattaatct gtacttcaaa ccttttcggc
      541 acaataaaaa tggcaggtag ggtaaaaatt gccatcaacc aaaatgtcag gcttggggaa
      601 tgttgataga ccattccggc aataaaggta aatactgcca taaaagcaca gcttgccaag
      661 ccaaaataga gaccttgtaa tttcgtcaca cgctctgccg attgcatcga aatatagcga
      721 atcatcgcaa tatgagccgc cccgaaggta atagaatgta gcatttgcat tacaataatc
      781 agcacgatct ctgtagtact acccattatt gcccaacgaa tcactgcaaa aacggcagct
      841 aaaatcatta aatggctgat tttccacgat ttaaacaagc gtttagagac caaaaagaac
      901 aaaatttccg ccaccaccgc caagccccaa aacaggctag tcattgcggt tgaaatactg
      961 gcatttgacc aatgaatggt gctgtaagtg taataagcgg cgtgcgaggc tgaaattaac
     1021 gacaccgcga tcaacatttt taaggtttcc ggctctttca ataattggcg ataagttacg
     1081 cttgaagtat tggctttatt accttgttct tcaaacccga ctttgggcgt agcaagctgc
     1141 cctattccta aaaaaaccaa gaaaccgatg ataatccaca ccaccgaatt ttcaccaagc
     1201 aagccgataa gatagccggc agaaagtgag cctaccacaa acgccattga gccgaataac
     1261 cttgctctgc cgtaatccag cccgatctga tgttgccaaa ttgaggcaat gctatcggct
     1321 atcggcattg cccccgaatt gaacatttga aacaacatta acaccggaaa aagtagccaa
     1381 atgaaatccc ctgaaaacag caacgccgcc gcagccgcaa tattcagcca agttaaactg
     1441 cgggcggtgg gaatgagttg gctcacggtt tttacccgtt gtgaggcaag catactgccg
     1501 gcaaagcgga acaaatagcc aacggcggca aataagccga tcatttcagt cgaatagccg
     1561 tgatgtttaa gccacactgg caaaaacggc aacaacacgc cataggcaca aaaaaagcca
     1621 aagtaattaa atgccgccca ttggaacggg gaaactttca tcataacgat tgctccattt
     1681 ctctggctcg atgaattgcc gcccacattg catcatcaac ggttttagca agatcgtgct
     1741 gctcaaacac ctcaagggct ttcgccgttg tgcctccctt tgaggtcaca ttttcccgca
     1801 aggtcgaaag tggcgtagtc gggttcgctt ccaccaattt cgccgccccc aatgcgacag
     1861 attgtacaag cgttcttgcg tcctgctcgg aaaagcccat ttgcaccgca ctttgctgca
     1921 ttgcctccat aaaacggaag aaatacgccg ggcttgagcc ggttatcgca ataatgtggt
     1981 tgatttgctc ctcggtttcc acccagtaac atttaccaac ggctgacatc aactgttcgg
     2041 caaattggca agcggtcgga tttaccgatt tttttgcaaa taatccggtc attccttcgc
     2101 tgattaaaga cggggtgttt ggcatcgtgc gaatgatatt tttcgcactc ggcaacagcg
     2161 tttccaagcg ttgcacagaa atacctgctg ctactgaaat cacccatttg ttggaaaaat
     2221 caacatcggc aaactcggca cacacttgtg ccatcatctg tggttttacc gccagcacga
     2281 tcacgtcagc ctgctctacc gcctcacgat ttgcaaaatt tgtcgcaatt ccgaccgctt
     2341 gtaactcctc tcggcgagcc aaattacttt tattgcacgc aatgatttgt tcggcagaat
     2401 aaccgctttt taataagcct tgaataatcg catacgccat atttccggtg ccgataaagg
     2461 caagtttttt ctcgttcatt cgcaaattct ctactgtgtt gggttgaggt ctgttaaaat
     2521 aacactattc tacacccgat aaaggatatg caaaaatggt ttggttcaaa aatgtgatga
     2581 tctataaatt aaccggcgag ctggcgttgg aaagcaatag ccttaacgag ttacagcaag
     2641 aaacccgctt taccccttgc agccagcacg atatgagcaa attcggctgg gctacgccac
     2701 ttaccggctc ggatttactg catttctcac aagcgaacca atttttgctg atttcacaca
     2761 aagaagataa attattacct gccaatgtgg tgaaagccga aaccgagcaa cgcattgcag
     2821 aactggaaga aaaagaggct cgtaagctga aaaaaaccga aaaacaagcc gtgaaagatc
     2881 aggtcgtttc tgcgttattg cctcgagcat tcagcaaaca tcaattcacc gctctttggt
     2941 tggatttaga taatcagttg gtttatgtgg atgcagcctc gagcaaacgg gcggaagata
     3001 cgctggcgtt actgcgaaaa acacttggct cgttgccggt tgtgccgatt tcctttaccc
     3061 taccgccggc agaggtaatg acgaattgga tcgccaaagg gcatacgcca agttggttga
     3121 atttgttgga agaggcagaa ctgaaatctt tcgataccga cagcattatt cgctgtaaac
     3181 gccaagattt agagacggaa gaaatcgccc ggcacttgca agcgggcaaa tttatcacaa
     3241 aacttgcgat tgaatgggaa gggcatttca attgcgtgtt aaatgaagac gccacgcttt
     3301 cacgcctgaa gtttgccgat gaagtgcgag agaaaaatga cgatattctc aaagaagatg
     3361 tggctcaacg ctttgatgcc gatttcttat taatgaccga agagctgaaa cttttcaccg
     3421 aaaaaatgat tgccgaattt ggcggaatta aatccagaat ttagaaaaaa atttaaggaa
     3481 agcaatgtta gatcaaacca ttatcgacca atatgacgcc ttgatttttg atatggacgg
     3541 cacattaatt gacacgatgc caagccacgc caaggcgtgg gaaaaggtgg gcgaagtgtt
     3601 aggctaccca atcaatccaa agccaatgta cgaactgagt ggctcaacca cttttgtgat
     3661 tgcccgagaa ataatgcaac gctccaatat ccccgagcat tattttgagc aagtggtaca
     3721 actaaaacgg gaatttggga ttgaaatggt gttagccaat gccactctgt tgcctgcctt
     3781 tgagattatt aaagctaacg ttggcaaaaa accaatggca atcggcacag gctcacaccg
     3841 tgcaatggtg gaattattag accaaaaatt taacttacgt caatatgttt cggttattgt
     3901 agattctgat gatgtaagca accataagcc agcccctgaa acctttttaa aatgtgcgga
     3961 aaaacttggc attgccccac accgctgttt ggtgtttgaa gatgccgatt taggcgtgca
     4021 agccgccctc aacggcaata tgaacgtgtt tgatgtacgt acgaatacga taactcactc
     4081 aacccgttaa aaatgatagg tttatatgaa aacagaatgg attaaacatt taattcaaca
     4141 attaaccacg ctttccaaag ccatcgaaaa ctactttacc agcggttcat ttggggtgaa
     4201 attctaccct tttctaatgc acccttgttt tcgtaaaagc tgtgcctttt tgatttttat
     4261 tattgctatg gtttcaagtt ttagcgggct attttatttg gtggcggaac acatcacgat
     4321 tttatttagc gaaagatcgc tcaccactta cttccaccat agcacccttg aattgatgat
     4381 tccagtcggc acaatgatag atgaaaaaat cagcgaatta tcaccacttt cggtggtaat
     4441 gcgttcaatg tttgtgctac aagcagtcgt ttttttcttt atttatgcaa tcggcatcac
     4501 ccacattgct cacaacaaat tgcgggtcat cggcttattg ctttcgctat gctttgcgat
     4561 tggctgctca ctagttgccg gtattcagcc aagcagtcgt ggcgaattcg gcatttacaa
     4621 tttaggggca agcattacgt ttttaatcgg caatttaacg ctgatcatcg caggcttcga
     4681 tctctaccac ccttcaatgc aattttttaa acgctttagc attggtgcgg gcgttatcgg
     4741 ggcaatctgt attttaatca cgattttcct accaaccctg tttagcccgt taattgaacg
     4801 agcaggtatt tataccatta tgctgtggga aattttagcc ggttttgcca tcattaaacg
     4861 cctgttgaaa ttgccctcat tcagacaaca aggataacca atgaaacaga ttccctttat
     4921 tcaacgctat gctcaactga ttgagctacc gaccatttcc agccttgttg ccgaagaaga
     4981 tttatcgaac cgccgtttga ttgaattgct cgccacttgg ctggcagatt tcggctttaa
     5041 aaccgagatt ttagcggtag aaggcagccg taataaatac aatttacttg cgacctatgg
     5101 cgagggtgag ggcggcttat tattggcagg gcataccgac accgtgccgt ttgatgaagg
     5161 aaaatggcgc ttcaatccat tccaattaac cgaaaaagca ggcaaactct acggcttagg
     5221 cacggcagat atgaaaggct ttttcgcctt tgtggtggag gttgtgagcc aactcgacct
     5281 aacccaaatc aaaaagccga ttcgcattct tgccaccgct gatgaagaaa ccacaatgct
     5341 gggggcaaga acctttgctc agcacagcca cattcgcccc gattgtgcga ttattggcga
     5401 gccgacctcg ctcaaaccga ttcgagccca caaagggcat attggcgaat cggttcgcat
     5461 taccggcaga agcggacact ccagcgaccc cgccaaaggc attaatgcga ttgaattaat
     5521 gcaccaatct atcggttatt taatgaaaat gcgtgatgaa ctgcgagaga aataccacaa
     5581 tccgctgttc caagtcaccc acccgacaat gaacttcggc aatattcacg gcggcgatgc
     5641 cattaaccgc atttgtgcct gttgcgaatt gcaatttgat atgcgaccac taccgaatat
     5701 ggcggtgcaa gatttgtatg caatggtaaa cgagcattta aaaccgatgc tggaacaata
     5761 tggcgattta attgaaatcc gccatttaca cgatggaatt ccgggctacg aatgcgaaca
     5821 ctcggcacaa atcgtgcaag tggttgaaaa gctactgggc gaaaaatgcg atgccgtcaa
     5881 ttactgcacc gaagccccat tcattcaaca actctgccca acgctggtac taggccccgg
     5941 ctccattgaa caagcccacc agcccgatga gtttttggaa atgaaatata tcgaaccgac
     6001 aaaagagtta ttgactaaat tgatttatca tttttgtgga taaatcaaca gaaacaagcg
     6061 ctcattttcg ccatactttt tgcaaaagaa tcggcaaaaa tgaccgcttg atatttagta
     6121 ttgagtgacc tataaacttc catacttcat cattgattta aggagattct gttagatagg
     6181 tattaatctc tagctaggtc ttggatacaa acggcgagaa tgaattatag taataatata
     6241 tactttttct aggtgtatct catagacaat acgataattg cggcagaatg tttctaatgt
     6301 gccatcttct ctacgtcgcc ctattgtagg cataaatgca atgagatcga gtttattttg
     6361 taattgttcc caaagtgaaa ctgcgctcaa ccaatgccct gtataatctt ctaccgactg
     6421 taaaatctca atgacattag accttgcggt aagtgacagc tcccaagatt taggcataat
     6481 taacctcaaa caaattttca ttttctcgta attgctgacg tttctgcata tccgcaaata
     6541 aatgatttaa ttcgttattg atttgttctt tggtaaaggt ttctcctcga gctattgctt
     6601 cttgaccagc tttaatttgc tctgcaagaa actcatcata tccttgttct ttgaatagca
     6661 tattctgctc ctttgatttg tgtcttgatt ccactatgtt caagacatag tttaagttta
     6721 aatctacgct caaagcaatg aagagtcaag ggaatcctta tgttgatagg gtgtgcatat
     6781 ttttcgagtc aaatgaatta aaaatcatca ttacgatcac ccactcatag attttatctt
     6841 cctatctaag gtcatcttct tatatcaaac aagcggtcta aatttgcaaa aaaatgacaa
     6901 atttagaccg cttgtgagtt acaaaaatat aaactaattg agcttatcaa ttactgcgca
     6961 taactaaata ctttcaccac tttggttact ccacctacat tgcgagcggc ttcggcggcg
     7021 gcatcagctt ggttttggct gactttgccc attaaaaaca cttcgccgtt ttcggtgacg
     7081 actttcactt cggtggcttt tacctcgcta tttactaaca gtttggattt cacttgggtg
     7141 gtaatccagc tgtcttgtgt aatctgtcca aagccgattt tttcgccttg gcggatttcg
     7201 ttgtaaattt ccgtcacgcc ctccgcccca gcagcgaggt ttttcgcatt ttccactacg
     7261 tccatacttg gtgcttgccc gattaataac actttgccgt tgtaagccac cacgttaatg
     7321 cgggcttctt gtttgatttg ctcgtcttta ttaatgttgt aagcgacttt ttcttctaaa
     7381 atttcatcat caacttgcgt gccggtggtg cgtgggtctg tcgccacttt agttgcaacc
     7441 gccgcagaag tcaccaccgc cgtggtgata caaccttgta aagagataaa acctaatact
     7501 aataggctgc cctttaatag tttatttgct aatttgttca taatctctcc ttacgaaaat
     7561 agtaaatggt cgataagttc acataataaa ttcacataaa aatgatggct ttcaatcacc
     7621 ctcagttgat tggttgaagg ggctgaaatt tcaatatcac tttcatctaa taagccgagt
     7681 gtgtggtcgt tattactgcc tgttaaagca ataatatcta aatctaaatg ttgatttttc
     7741 gccgtatgaa tagcttgcaa tatacattct tcattaccca caggcgaaaa aacaatgaag
     7801 agatcaccct cttttgcaac cgcctgcaac tgttttttat aaacagcctc taactcgtta
     7861 tcttgtgcca caactcctgc caatacaccg cttaattgca gttgtacggc agcaaaactc
     7921 ggtcttgcca gttcataacg gtgaagtaag tggctgacca ataattcggc attaccgtaa
     7981 gagcgaccgt gtccgcagac gataacttta tcgccacgca acaaacaggc aacaatgcgg
     8041 tttgccgctt cacttaattt agccggcaat agctcagagg cggaaatttg ggtttgaata
     8101 ctttcggtaa atctatcttg gattttattt aacattcttt ctaacctaaa aagttgggaa
     8161 tccacacagg cgtttggttt ccttcaaaag caaccacatc aaaacggcaa tttgcagttt
     8221 ctaaactttg gttttgttta agcaaccaca tattagcggc attttgccat ttttgttgct
     8281 tgcggtaatc aatactctca acggctgaac cgaagcggtt gcttttacgc tggcgaactt
     8341 ctacaaacac aacggtttgt ccgtcttgca taatcggatc cagttcgcca cctttaaatg
     8401 cttggttagc ggcaacaaaa cgaagccctt gctgttctaa aaacactcgg gctttttgtt
     8461 cataaaacga gccttgttcc cgtttattaa taagcttgtt gaatagctcc attgcgatat
     8521 tgcatccaag ataaactacg ttcaatatta caatgttgtc cggcttttaa ctcaccggtt
     8581 aaacctgaaa ttttgtagcc cggaatatgg cggaattcat tgaatttggt tgccaatgac
     8641 cacgcatcag cccccattgc gtataaacgc atcattgaga aatcgctatt ggctaaattc
     8701 tcggcttttt tatattcact agaactttga tcagctaaca gtggcacttc actaaatttt
     8761 accccttcca ttgaagtgta aaaatcaatg ccgttatttg gcgaattact gcgagatgag
     8821 gtatagatcg ctaaacgatc ttttaaggtt gagctatcaa taccttgttt aatttctaat
     8881 acttgctcgg cattccccaa aatatataaa gctgctttgc tcacacctgc attttgaata
     8941 gcggcaacgg catcaagcgg ttggttgtag taacgcacat cagcatcgct attggtaagc
     9001 tgacgccagc gtttggcaaa ggcttctgcc gagcggttgc cgtagtcatc ttgaggggcc
     9061 gcaacgatag cgacactatt accgtcttga taaatttttt ccgcccctga ttgtgcctca
     9121 gattcaggag ataaaccgta gtaacacacc tgagcaatat tacgcacatt ttcagtggca
     9181 ttcagtgcca aaatattcac gttttggatt tctgggctaa gtagcatttc atcaacacga
     9241 gatttgagca atggcccgac aatcacattt gccccttgct gttttgcctg tgaaattaag
     9301 gaattaacat cagatgaatc ggtatcaaaa gtttgcactg cggttgagtc atcagcctta
     9361 gcatcattaa aaccacgctt gataatatca cccaaaattt ttgcatcacc acttagaggc
     9421 aatagcaatg cgacactgtt taactgggtt tgttggaaat tactcacccc ttgtaattcc
     9481 gttggcatta cggcaaccgc actatggctc gggtattggg ctttccaatt attaattgct
     9541 tgtggcattt gtgccggtgt cgtcacattt tgattataga tattaattaa tgccaaccaa
     9601 ccagcaagct ccatctcacc cgcttcaggc acagtttttt ccagcattcc tcgatttgca
     9661 tcacgcaagg tttgccaaat aatatcgtta ttttcttggc gttcacggtt cattttatag
     9721 aaattgttaa gttgcgatct tgcacgcaca atgccaatca catctttctg attggcagca
     9781 atacgggctt gggtttgaat ataacgcaaa cgctgtgccg aacttaatag cggatcttga
     9841 atggctttta attggcttgt tgcttgctcg tttttccctt gtaaggcggc aagctgggcg
     9901 gaaaccaagg catactctaa cttttgcacc tcgttcatct ctgcggtcag aatttcacta
     9961 aaggtatttt gagcctcata ttccttattt tcatcaatta atttgcgtac agctaaaagt
    10021 tgatacgaaa ttttatcttc cgcttctttg gtttgatctg ctttattaat atagaattcc
    10081 gagcttgaat aggcttcatt tttaatggat tcggtgacag gatttaccga tgtacaggca
    10141 gaaatgaata atgcaatcgc cgttggcacc aatgcctttt tcattgtatg gtttaaaata
    10201 gttgccattg tcagatcctc aatagaatat aatgtgctaa atttaatgtg aaatcttact
    10261 tatcaaacga gctaaaagca aatgaaaaac agacaaaatt catctggaat caacccaaat
    10321 tacggaattt tatatattgt tgccacgcct atcggcaatt taggcgatat tacccaacgg
    10381 gcgttagaca ccctttcatc tgtcgattta gtcgccgccg aagacactcg ccacagcggt
    10441 ttattgctga gccactacgg cattaaaaaa ccgtttttcg ccttgcacga ccataatgaa
    10501 cagcagaaag cggtcgtttt agtcgaaaaa ttagcaaaag gggaaaacat tgcgttaatt
    10561 tccgatgccg gaacgccact gattagcgac ccgggctttc atcttgtccg tcattgccgc
    10621 caagccgggg taaaagttgt gccgatcccc ggggcttgtg ccgctgttac tgccttatgt
    10681 gcttcaggca tagcttcaga ccgtttttgt tttgaagggt tcttaccggc aaaaacaaaa
    10741 tcccgttgcg ataaacttac cgagcttgaa aacgagcctc gcacactgat tttttatgaa
    10801 tccacccacc gtattttaga cactcttgcc gatatgcaga ctgtttttgg cgaagatcgc
    10861 tatgtggtaa tggcgcgtga aatcacgaaa acgtgggaaa ccatttatgg agatcaactg
    10921 ggtaacttaa ttgaatggct aaatgaggat agcaaccgaa ttaaaggcga aattgttctg
    10981 gtggttgaag gtaaaccgca aagtagtgag gaagcctttt ccagccaagc gatcaaatta
    11041 ctgacattac tctgccaaga acttccgctt aaaaaagccg ctgccattgt ggcagaaacc
    11101 ttcggctata agaaaaatgc cctgtatcaa tatggattgg agcattttga gtaacagaca
    11161 agcggtcaga ttttgtgagt tttttgcaga ttggcactcc cctctctctg aaaaatgttt
    11221 ctgaacgaaa catttcccgg tctcaatact cgctacgctc gctccttcgg agccgttggc
    11281 aaagccaacg ttcaaatgta aacatttgtc ccataagggg agagagctaa attattcgct
    11341 tgtcgacccc tctccactcg tgggagaggg acagattctt cttcgttcag aagaaaaatc
    11401 agggagaggg ggaagtttct cgcactaaat tccgataagc atatgccata atcgcaaaca
    11461 tcaacagcaa gtaaagcggt aataattgaa tgctgatagc attagcaata aagccgaaaa
    11521 tcggtggcaa taacaggacg ccgatatagg cacttgccat ttgcacgccg atcaccgatt
    11581 gagacttgtc ctcgccgaaa aacgcagggg tcgaatgaat taagcacggg aaaatcgggg
    11641 cacaacctag cccgataata attaaacttg ccagcgtaac atatttgcct aacggcaagc
    11701 acagtatcgc aatgccgact aagatcaagg ctgaaccaag tttaatcatt tgtctgttgc
    11761 taaatttaag cgtcaagaag ccacttacgc ctctgccaac ggtaatgccg ataaagaaaa
    11821 tactgccgaa cgcagccgct tgttctaagc cgacatcttt aaccattaca aaatagctac
    11881 tcgcccacag tccggcggtt tgttctacgc cacaatagcc aaaaaaagct aaaatgccat
    11941 atttcacgcc cggaattttt agcgtttctt ttaagctcat cggttttgcc gaatggttga
    12001 tttcagcggt aatctgcttg cgtaatttcc aaaaaggcag tgagcaaaac agcattagcg
    12061 ttagcccgat ttgtaacatt gaaatggtga aatagcctgc ctcccagcct cgcccttgca
    12121 ataaggcgta gcctaaaatg tgcggcccga tagaagctcc aacgccccac atacaatgca
    12181 gccagctcat atggcggctt gcgtagtgaa ctgctacata gttattcaac gccgcatcaa
    12241 ccgctccagc ccctaagccg taaggaattg cccataaaca taagatccag aaagcatcac
    12301 tgattgaaaa gccccacaat gctaaagccg ttaaggcaac gctgatgacc attactttgc
    12361 ctgtgccgag atggtaggtc agtttgtccg acattaagct agaggtaata gtgccaatcg
    12421 acataatcat cgagaccaca ccggcatagg aaagcggaac agcaagctgt tggtgcattg
    12481 acggccacgc cgagcctaaa attgcatcag gcaacccaag gctgataaaa gagagataaa
    12541 taatagcgag taggaggtta gtcatagctt acaatcggtc acaaaaagca tcgctttttg
    12601 aacgttggct ctgccaacgg gagcgaagct gtgaacaatc cttacaagga ttgtgaataa
    12661 tttttgataa attttttgta aaaaaatggc ttaaggagac cttaagccaa attttattat
    12721 ttcacttctg ccaaataggc cttattagca cggtcaaagg tgtctaatac ggctttgctt
    12781 ggcattggcg tgataagtga taccacaatg atcattaggg tagcagcaat aaagcccggg
    12841 atcatcgagt aaatttccgg taaggcgagc ttaagtgtaa tatcgcccca aaaatgcacg
    12901 attaacgcac cggtcagcat tccggcaatc gccccaaaac cgttcatacg tttccagaat
    12961 agtgaaagca ataccacagg cccaaatgcc gagccgaaac ctgcccacgc attacgcact
    13021 aagccaaata ctttgctgtc cgggtcttgg gcaatataaa tagctaatgc tgccaccgca
    13081 agcaccatca agcggcttaa ccaaaccagt tctttatcgg aggcttgagg gcgtaaaatg
    13141 cctttgtata aatcttcggt aatcgcactt gaacaaatca gtaattgagc ggaaagggta
    13201 ctcattaccg cagcaagaat ggcagataat aaaatgccgg ctacccaagg gttaaataac
    13261 aatttagcta attcaatgaa aatttgttcc ggatttttgc tgatattgcc gacttgctcc
    13321 gggttgccaa agaaatatgc cataccaaaa taaccaatgc ctattgcccc cagtaagcag
    13381 ataatcatcc aagtcatact gatacggcgt gcattttcaa gagacttcac actttctgcc
    13441 gccataaaac gcaccacaat atgcggttgt ccgaaataac ccagccccca cgccattaag
    13501 ctgataatgc ctaatacgct ggtgccggtg aaaatgtcgt tgtagtcttt ttgtgccatc
    13561 tcgcctgcac ggtcgattaa ggcttgcatt tcatcaaaac cacctaagtt atacatcaca
    13621 aaaaacgggg tgagaatcag ggcaaaaacc attaatgaag cttgaatagt atccgtccaa
    13681 cttactgcta agaaaccacc aataaaggtg taaagaatgg tagcaacagc cccataccaa
    13741 attgcttggt ggtaaggtaa gccaaataag ttttcaaata ccctcgcacc ggccacaacg
    13801 cctgatgcac agtaaattgc aaaaaagagt aaaaaaatga ccgctgataa aatcttaatt
    13861 aaacgggttt tatcgccaaa acggttatga aaatattcgg gtaaggttaa ggcatcgcca
    13921 ctgtgttctg tatgcgagcg taatctgcct gcgactaatt tccagtttag ataagcaccg
    13981 atagttaaac cgattgcgat ccagccttcg atcaaaccgc ctgcataaac ggctccgggt
    14041 aatcccatca gcaaccagcc ggacatatct gatgcaccgg cagaaagtgc ggtcacaaag
    14101 ctacctaaac ggcgaccgcc taaaatatag tctgaaaggt tattggtaac acgataggca
    14161 ataaagccga tggcaatcat tcctagaata tagacaacaa acataattgt tgttggatca
    14221 agaccaaaca ttattaatga atcctcaaga tttcttattg tataaagatt aaagtgagta
    14281 aagggtaaaa gattacctga aataatcaaa atggcaacac aaaattaccg tccgcccagc
    14341 tcagctgaaa ttttttctgc tgtttcttga atagccggta gtagattttc ccgccccagc
    14401 tgatttaaac gagcagttga aagcgaaaca gaaatcgcat aatggacttt ttgttggtga
    14461 tcaaaaatcg ggcaagcaat acaagaaacc cctaattcat tttcttcgtt atccattgca
    14521 aaaccttgac ggcgaatggt ttccagctcg gtttgcattc cgtcaatcgt ggtgatggta
    14581 ttgaccgtca gtttttgaat gatgtccgat ttagtctgcc aatattgtgg aatgtaatct
    14641 gccgaaccat aagcaagaaa cagttttccc attcccgagc aatagagttg catttgttgc
    14701 ccgatatagg ctcgggtacg catcattccg gtggtagctt ctaatttatt gattaaaata
    14761 gcgtgatcgt tttcccgcat cgcaaaattt accgtctcgc ctaaggttaa atttagggct
    14821 tctaaatgtg gggcggcaat atgcaaaatg ttaatcgaac ttaaggctcg ctgcccgatt
    14881 gccaaacatt tggtggttaa acgataactg cccgcagtcg gtgcagggcg gacgtagccc
    14941 tcttgttgta agccttgtag cagacggtga accgtgcttt tgttcagccc cgaaagctcg
    15001 gctaaatggg caagcggaca gccgtttggg aatttcgcta acaattccaa tagtgttaag
    15061 ccacgaatta aactttgatt tccactcgat tccttttctt tctttgcatt catttggctg
    15121 ctcccaaaat aatatgatct aacggcatat cccaactctc aatcggtaat tgttcgactt
    15181 gctgacattc gtgtgccaaa ccgacactaa tcagatgagg tgcttgagca agggttctat
    15241 cataaaagcc acctcccatt cccagccgat ttccggcttt atcacaagca acaagtgggg
    15301 taaaaatcat ctctaattct tgcaacggaa ggatatttct aacatctaat atcggctctt
    15361 gaatccccaa gcggttaaat tttagaatag ttttgcaatt gtaatttaca aacaataatt
    15421 gaccgcttgt aaaagggtgc aacagcggca gatacagctt tttgccctgt tgaagtaatt
    15481 gttccattaa aggaatagga gaaatttcac cgttaaaagg caaataaaag gcaatatgtg
    15541 aggcttggta agattcaatc aatgaaagag cttgaggaat aatggattcg gctgcttgag
    15601 tttgctgacc ggcagttaaa ctttgccgtt taagccgcat tgattgccgt aattggctac
    15661 gaagatcggt taaagatgat tgattcatat aaagacccga gatgccgcta ccagtaatgg
    15721 tccttgaacc agtcggttca agggagtcaa acttaactgc ttttaggctt cttactccta
    15781 attaaattag ggtaagcttg ctcaccagcg ttagatgtcc aactcaaagt ttcaaattat
    15841 cggctcaggg acgtaactcg taacgcacac cccaggcaag acttatagta acggaatttg
    15901 atgatttttc ctagtattta atcccacaaa atgcaaattc tcaatctttt tggctctatt
    15961 cctaatcaat tcgattaaaa attacctaat tggattgaaa tttgccaagc gaattatcta
    16021 actgctcaat acaagccacc aacacatttt tattttctgc attttggcgt tgggcttgct
    16081 ccagttcata atttaaattc agtgccacaa tcgctaacac tttttcaagc tgaataatgc
    16141 cggactgctc tttcaatacc accacacgct cttccaaacg ctgtgctgaa gccagtaaat
    16201 gctcttgctg ttcaacaggg cattgcaagc gtaaaacctg cccaaaaatc tgtagttcga
    16261 tattatttgc cgacataaat acgaaatcct cacttaatcg caaaattttt gctatattat
    16321 gccattcttt agcaaaatca caaataatcc cattatggct atcacagatt cccaacaatt
    16381 taaacaactg gtatcacaat tacaaattga tgatacaccg gcagagtttc acggtttttt
    16441 atgcggttta attgccggcg gtattcaaga tgagtcttgg aaaaccctca cctaccaatt
    16501 taccaatgac ggacacgcat tttccatcga gccgttaaac actttaaccg atttttatca
    16561 gcaattaagt gaaagctttt cagaagccaa cacgctgttt agcctttggt tgccacaaaa
    16621 cgaagaagat ggttttgcct tagctgatgg tatctccgaa tggacaaaca gtttcttact
    16681 tggacttggc gttgctcaac ctaaattaca acaagaaacc gatgaggttg gcgaggcaat
    16741 cgatgattta gatgaaatcg ccaaactagg ctacagtgac gatgataata acgaggaact
    16801 actggaggct ggcgaagaag tgttggaata tctgcgagtt ttagccttat tcttgcacag
    16861 ccattttgct ttaggcgtgc cgaaaaccga agaaaagcca ccattacact aatgaataaa
    16921 tacaagcggt tcaatttcat ctctattttg caaatatatt atgcggggcg aaaaatattt
    16981 cgcccctacc aaaatcaaag gaaaaaccaa tgtcagaatt aaaaaacgat cgctatttaa
    17041 aagcgttatt gcgtgaacct gtggatatga ccccggtctg gatgatgcgt caagccgggc
    17101 gttatttgcc ggaatataaa gccacccgag cagaagccgg cgattttatg tcactctgcc
    17161 gtaacgctga tttagcgtgt gaagtcacct tacaaccgct acgccgttat gaactcgatg
    17221 cagctatcct attttccgat attttaactg tgcctgatgc aatgggatta ggtttaagtt
    17281 ttggtgcagg tgaaggccct aaatttgccc gcccggtggc aagcctcaat gatgtcaatc
    17341 agctaccgat tcctgatccg gaagacgagt tgcaatatgt aatgaatgcg gtgcgtacca
    17401 ttcgccgaga attgaaaggc gaagtgccac taattggttt ttctggcagc ccttggacac
    17461 tcgccaccta tatggtggaa ggtggttcaa gcaaagcatt taccaaaatc aaaaaaatgc
    17521 tttacgctga gcctcacatt ctacacaaat tgctggacaa gttagctgac agtgtcatct
    17581 tatacttaaa tgcccaaatc aaagccggtg ctcaagcggt gatggtattc gacacttggg
    17641 gcggtgtatt ggctcacgag gattacaaag ccttctcatt acactatatg cacaaaattg
    17701 tggacggctt aattcgagaa aatgaaggac gtaaagtgcc tgttacccta ttcaccaaag
    17761 gcggaggctt atggttagaa gcgattgcag aaacgggctg cgatgccgtt gggctagatt
    17821 ggacagtcga tattgccgat gctcgccgcc gtattggcga caaagttgcg ttgcaaggca
    17881 atatggatcc aagcgtgctg tatgcaagcc ctgaacgaat cgaacaagaa gtaaaaaaca
    17941 ttcttgccgg cttcggcaac ggcagcggac acgtctttaa tcttggacac ggcattcatt
    18001 tagatgtgcc ggtggaaagc ccgaaagtgt ttgttgatgc gattcacaac tactcgaagc
    18061 agtatcataa ataatggata aaatgccctc tctccttgtg ggagagggac agatttttct
    18121 ttgttcagaa gaaaaatcag ggagaggggc aagcggtcat ttttatacag aattttgcaa
    18181 aatcgttccc ttctctctga aaaataaaga aggaaataca atgagaaacc cgattcataa
    18241 acgcatggaa cgtttcgagc cttggcaaaa caccacattt atggcgtgcc tatgcgaaag
    18301 aatgtacccc aactatcagc ttttttgtga agtaaccgaa cagcctgaaa aagctaaggt
    18361 attccaaaat attttaaatt tagtctggga atatttaacc ataaaagggg caaaaattaa
    18421 tttcgataac caactggaaa aattagaaga aatcattcct gatgtgaacg aatatgcgtt
    18481 ttttggtgtc ttacctgcac aggaagcttg cgaagccctt tctgaacttc ttcacagtat
    18541 tatcgctggt tcaacccttg aacaagcaat ccgcatcagc caaatttcat taggcacagt
    18601 tgccagctat ttggaaatgc aacaagataa agaactgaac gagcaagagc taaagaattc
    18661 tgaagaaatt caagaagaac tggacgtaca gtggcaaata taccgcttgc taaacgaatg
    18721 tgaaaaacgt gatcttgatc tcatttttga acttaaagat gaaatcagaa gcagcgggat
    18781 tagtaatatc gggttaaata ttaaccaata gcattgatat ttgcaaaatc ttgcttaata
    18841 atactttgag aaatcttaac tttgaattaa gctatcgcaa ggtttcaata acttctcaga
    18901 cgttttacgt catttattaa aaacacatat cttagaggat aaaactatga acaaaactga
    18961 gttaatcgat gctatcgccg ctggtgcaaa cttaaccaag aaagatgcta aagcagcatt
    19021 agaagcaact cttgatgcaa tctctgcaag cttaaaagca ggcgacgctg ttcaattaat
    19081 cggcttcggt acttttaaag taaatgagcg taaagcacgt actggtcgta acccaaaaac
    19141 tggtgaagag atcaaaattg cagcagctaa agtacctgca ttcgtttcag gtaaagcatt
    19201 aaaagattta gtaaaataat tctttaaaga tataaaaata aagcctggat tataatccag
    19261 gctttatttt tagcgtgttt cgatagctac agagaaacta aaaagcacta tttgctttca
    19321 atcgcttttt tcatttcttc aacttttgtt tctgtctcaa ctttcacttc tgctgccttc
    19381 tcagctactg cctttttagc ttcttctgct ttatctgctg ttgcttgagt cgcatcactt
    19441 gctgctgctt ttagttcttc cactttagct tcagttgtgg cttttaactc agcagctttc
    19501 tctgctactg ccttttttgc ctcttctgtt ttatctgttg ttgcttgagc tgcatcattt
    19561 gctgctgctt ttagttcttc cactttagct tcagttgtgg tttttaactc agtggctttc
    19621 tctgccactg ccttttttgc ctcttctgtt ttatctgctg ttgcttgagc tgcatcattt
    19681 gctgccgctt tcaactcttc aaccttcgct tcagtagccg atttaacttc tgcagctttt
    19741 tcagctacag cttcttttac ttcaaccgct ttgtctgtag ttgattgcgc ggcttctttt
    19801 gcctgatcac aagcagcaag tgccatagtt gtacctaaca ctaataatga taaaaatgat
    19861 tttttcatac cgagttcctt a
//
LOCUS       DANLUQ010000032        19863 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.032, whole genome shotgun sequence.
ACCESSION   DANLUQ010000032 DANLUQ010000000
VERSION     DANLUQ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 19863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19863
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.032"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..400
                     /gene="secE"
                     /locus_tag="PXB27_000896"
     CDS             <1..400
                     /gene="secE"
                     /locus_tag="PXB27_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDL5345712.1"
                     /translation="KTKTPEQVEQKGGKSKGANTALWLVAIVFLAVAAVGNAYFSSHF
                     SLIVRVLLLVVLLVGAVALAAITNQGQKAIHFIKESRTELRKIIWPTRPEATQTTLIV
                     LAMCVVVSLVLWGIDSIIVALITFVTSLRF"
     gene            402..965
                     /gene="nusG"
                     /locus_tag="PXB27_000897"
     CDS             402..965
                     /gene="nusG"
                     /locus_tag="PXB27_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDL5345713.1"
                     /translation="MSEVEVKENGSKRWYVLQAFSGFENRVAVTLREYIKLHKMEEQF
                     GEVLVPTEEVVENVGGKRRKTERKFFPGYVLVEMEMNDDTWHLVKSVPRVMGFIGGTA
                     DRPAPITKREADRILNRVQETAEKPRHRKEFQPGESILVTEGPFADFSGTVEEVDYEK
                     GRLKVSVSIFGRATPVELEFSQVEKQS"
     gene            1198..1626
                     /gene="rplK"
                     /locus_tag="PXB27_000898"
     CDS             1198..1626
                     /gene="rplK"
                     /locus_tag="PXB27_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDL5345714.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     RTESLEKGLPIPVVITVYADKSFTFVTKTPPAAVLLKKAVGIKSGSGKPNKDKVGTVT
                     QEQIRQIAETKAADMTGATIETKMKSIAGTARSMGLIVEE"
     gene            1631..2320
                     /gene="rplA"
                     /locus_tag="PXB27_000899"
     CDS             1631..2320
                     /gene="rplA"
                     /locus_tag="PXB27_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDL5345715.1"
                     /translation="MAKLTKKMKAIKAGVDSTKAYDINEAVEVLKKFATAKFVESVDV
                     AVNLGIDSRKSDQNVRGATVLPHGTGRTARVAVFTQGANAEAAKAAGADLVGMEDLAE
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKSGQ
                     IRYRNDKNGIIHTTIGKADFSAEQLKDNLNALLAALTKAKPTTAKGIFIKKVSISTTM
                     GAGVAVDQASL"
     gene            2382..2801
                     /locus_tag="PXB27_000900"
     CDS             2382..2801
                     /locus_tag="PXB27_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345716.1"
                     /translation="MLRLLSFCFIFLLSACTVLPQIKQLPPQVESVKSFKVENNGQQS
                     LLLIQFYENKWRWIQTDPLGAPIARVWLTKKGWEPDGFVMPNKQAQLLFTALVNALNP
                     HSPPFYLDKGWKIEREKSQFTIWLPDSSKWKVEILGE"
     gene            2888..4110
                     /locus_tag="PXB27_000901"
                     /pseudo
     CDS             2888..4110
                     /locus_tag="PXB27_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005544283.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
     gene            4103..4507
                     /locus_tag="PXB27_000902"
     CDS             4103..4507
                     /locus_tag="PXB27_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005557532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HDL5345717.1"
                     /translation="MHKFTFPIEQIAPLVPHSGNMILLDRITAFGEDFLTAETNIRPD
                     NPLIKHGKLATYASIEIMAQAGEPVRLGYLLGTRKLHLHRQEIPIGSQLQIQIKMSIQ
                     DATGFGVFDCQLIDLADNQVLLEGALNVFSPK"
     gene            4607..6040
                     /locus_tag="PXB27_000903"
     CDS             4607..6040
                     /locus_tag="PXB27_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HDL5345718.1"
                     /translation="MQEAYDIQHLCTRKEDKSFDRKSARKEPKEILRHLIGFANADGG
                     LLVIGIEDNGEITGFNYQKAHQPEEFIHALHNLQKMPIPVTYQQIEVVNSRGLADFVL
                     LFEVTASSGRVITSHDDRVFLRSKDQTLELNFEQRLQLEYEKGQRAFEEQPIARADFT
                     DLDMDLLEQYRQKLHTDRSIEEILKARDLINKEGQITNACILLFGNNPTRYFPNARLR
                     FLRYEGKAPLTGRTFNVVKEFMLEEPIPKLLESAKAVLKSQLREFQRLDENGQFTKID
                     EYPEFAWLEGIVNAIAHRSYSNQGDCIRISMFDDRLEIFSPGGLPRPVTLENMYYTRF
                     SRNPKIARVLYEFGWVKELNEGVNRMAEEMNAYQLNTPSYFEPNENAVRLILENNIEH
                     RTLRALERIEEIFTQDIMATLNKNEVTILQYLYENEMITVKIASEILDRSKELSRRIL
                     KSLEQKNILTWHGLSPKDPTQYYTLKK"
     gene            6054..6782
                     /gene="fabG"
                     /locus_tag="PXB27_000904"
     CDS             6054..6782
                     /gene="fabG"
                     /locus_tag="PXB27_000904"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012771807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HDL5345719.1"
                     /translation="MNNTVLITGSSRGIGKAIALTLAQQGFDIVVHCRSRVEEAEAVA
                     QAVRNLGKNARVLQFDVADREKVRSILEADVEQHGAYYGVVLNAGLTRDNAFPALTDE
                     DWDSVLRTNLDGFYNVLHPIMMPMIRRRKAGRIVCITSVSGLIGNRGQVNYSASKAGI
                     IGAAKALAIELAKRKITVNCVAPGLIDTDILDENVPIEEILKMIPAGRMGDPDEVAHA
                     VAFLMDEKATYITRQVIAVNGGLC"
     gene            6845..7990
                     /locus_tag="PXB27_000905"
     CDS             6845..7990
                     /locus_tag="PXB27_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014167571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HDL5345720.1"
                     /translation="MKRVVITGIGVVTAFGKEWAEIHGYQPPKHWNRKQLRSLGRVSQ
                     FVVEASERALAQAGLLDENGVIFDWLKDRRMGVACGSSTGSTNDIKDAAELLMTGKSD
                     GFNANTYVRMMPHTTAANIGIFFGLTGRIIPTSSACSSGSQGIGYAYEAIKYGMIPMM
                     LAGGGEEFCASKVYVFDSLYAASRRNDNPKGTPRPYDKDRDGLVIGEGAGMFVLEELE
                     HALARGANIIAEVVGYGANSDGSHVTRPQATTMQRCMELALKDAGIQPNQVGYVNGHG
                     TATEQGDIAETQATTAVFGKIPLSSQKSYLGHTLGACGALESWFSIEMMRDGWFAPTL
                     NLENVDERCGDLDYLQGDGREIHTDYVMNNNFAFGGVNTSLIFKRWK"
     gene            8004..8909
                     /locus_tag="PXB27_000906"
                     /pseudo
     CDS             8004..8909
                     /locus_tag="PXB27_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251927.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dialkylresorcinol condensing enzyme"
     gene            8932..10074
                     /locus_tag="PXB27_000907"
     CDS             8932..10074
                     /locus_tag="PXB27_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HDL5345721.1"
                     /translation="MLSNVYITNTSAFLPNAPVSNDAMEAVLGMVGETPSRVRKMILR
                     SNGIQHRYYAIDPITRQATHSSTELAVQAVKKLGNSATSATCLAVGTSYPDQIMPGQG
                     VMVHGLLDAEPMEVLTTAGVCVAGMAAMKHAYNAVRIGEHRAAIATASETASAIMRSE
                     HFRAEIDHKKLDDARPEIGFEKDFLRWMLSDGAGAVLLANQPNEKGLSLKIHWIDLVS
                     YANEMPVCMYAGAEFIDGNYTGWKAMSEAQRAECSAMAVKQDVKLLNENIVKYTVEKA
                     LERIIPKYKLNAEEIDYFLPHYSSGSFRDKLLEGLQRINFLIPQEKWFTNLTTKGNTG
                     SASIYIMLDEFIRTHDLKQGQKILCYVPESGRFSSCFMLLEAVVNS"
     gene            10123..10338
                     /locus_tag="PXB27_000908"
     CDS             10123..10338
                     /locus_tag="PXB27_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDL5345722.1"
                     /translation="MDINDIPQDNSKIFNGERKVIYARNPQPEIRQMISSILACYAWD
                     ENNPFVAKSDARLKALAEVVGKAEIGD"
     gene            10619..11110
                     /gene="rplJ"
                     /locus_tag="PXB27_000909"
     CDS             10619..11110
                     /gene="rplJ"
                     /locus_tag="PXB27_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDL5345723.1"
                     /translation="MALNLQDKQAIVAEVNEAAKGALSAVVADSRGVTVEKMTELRKA
                     AREAGVTMQVVRNTLLRRAVEGTEFECLTDTFTGPTLIAFSNEHPGAAARLFTEFAKT
                     NKEFELKGAAFEGKKQDVAFLATLPTYEEAIARLMGTMKEAAAGKLVRTLAALRDQME
                     AAA"
     gene            11169..11537
                     /gene="rplL"
                     /locus_tag="PXB27_000910"
     CDS             11169..11537
                     /gene="rplL"
                     /locus_tag="PXB27_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDL5345724.1"
                     /translation="MSLTNEQIIEAIASKSVSEIVELITAMEEKFGVSAAAAVAAAPA
                     AGAAAAEEKTEFDVVLAEAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGISK
                     AEAEALKKELEEAGAKVEIK"
     gene            complement(11644..12474)
                     /locus_tag="PXB27_000911"
     CDS             complement(11644..12474)
                     /locus_tag="PXB27_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL5345725.1"
                     /translation="MVQKPLNHYLEMQEHFLYVPYLHHHRRIRVLLPKDYHNEGWERY
                     PVLYMHDGQNVFYSKESYSGHSWKIIPTIKSHQELPKMIIVGIDNAGANRLNEYGPWK
                     TTTGSTPETRNAGGLGEEYAKWVVETVKPFIDIHYRTKPQQEHTLLAGSSMGGIITAY
                     MGSRYPHIFGHLGVFSLASWFSEPDFLRFTHQYPLQPNTKVFIQVGTNEGDEIDSHFI
                     SNTNQTYIDCSLNYYQALIRIGVPLDNIRLRIMANEIHHEMHWADHFVEFLHFSLLRK
                     "
     gene            12700..14241
                     /locus_tag="PXB27_000912"
     CDS             12700..14241
                     /locus_tag="PXB27_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HDL5345726.1"
                     /translation="MQASLQDILEVVKAKNLTYQQKLMSLGNIAERLFNPIELLGYTE
                     EEWEHIENQMICDLNEGYAIYRPRYILPDYNVYMQKGCQFLDLPPPTNLDEALDGLLI
                     LYSHVPSITTFPVYIGRLDQLLEPFITDEEQDYIKIKRFLNHIDKTISDSFCHANIGP
                     NDTKAGRLILKAVIELENITPNMTIRYDKTKTSREFAELAAKACLLVSKPSFANDAYY
                     IQDLGEEYGIASCYNALPECGGAYTLTRLRLGTIGRACASVEQMVNEMLPKVAKLALS
                     TMDKRHKFLVEESNFFESSFLVQEGFIKRTNFTSMIAIVGLADAVNHLLKQEGLNETF
                     GQSKRGDEIATLIMDKLQAVNDAHKGLYVERTNNQYLLHAQVGANNHAEDKMNTPAHR
                     IRVGEEPTLLAHLKQSAPFHKYFPSGTGDLFAFDQTYTDHLDAVVDIIDGSFAQGYRY
                     ITTYLKNTDLIRVTGYLVKKSEVERYRKGEAVLRDTTWYGSGTDDCAKVFDRQLRDEE
                     NVNAG"
     gene            14244..15032
                     /locus_tag="PXB27_000913"
     CDS             14244..15032
                     /locus_tag="PXB27_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HDL5345727.1"
                     /translation="MAALSEIFVPLHRIIPFSNVEGQGNRTSIFLQGCKLNCLYCHNP
                     ETIPRYSKEAKQVSLQYLYDQVMEAVPFIRGVTISGGEPTIHHKKLVPLFEALREQGL
                     TCYLDSSGFFDFEATEPLINVTDKFLFDLKGDGAGLQTLCFDRKNQAGKVPEQALPNV
                     NHIKPENLARNLQNLAKLLPLDKVEEVRLVYLNEFYNAHHLLEKVADLLKPYPDVLLK
                     IIRVHTKGARDETGLARFVPTIEQTDELAAFAKRHGIQKVITIY"
     gene            15045..16302
                     /locus_tag="PXB27_000914"
                     /pseudo
     CDS             15045..16302
                     /locus_tag="PXB27_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005760733.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
     gene            complement(16381..18390)
                     /locus_tag="PXB27_000915"
     CDS             complement(16381..18390)
                     /locus_tag="PXB27_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL5345728.1"
                     /translation="MSAENMPSVIRFEQAVAKKDYESACTELLSILSKLDSNFGGISN
                     IELNMPEQIENLENDKAIYFCTRMAVAITRLFEDPALEISEHGAMRFLTLQRWIALIF
                     ASSPYVNADHILRTYNRNKESANPNTVDLDATLQALIKFCILYLPESNILLNLDAAWN
                     ASSDLTASLCFALQSPRFIGTSSAFAKRAAILQWFPEKLAQIENLNKLPSAISHDVYM
                     HCSYDIEANKHNVKRSLNAVIRRHLLSVGWEDRKIEQLGTRNNKPVMVVLLEHFHSSH
                     SIYRTHSTSMVAAREHFHLIGLGSDAVDEMGQQVFDEFHLLPQDGSLFDRLSFLKDIC
                     DKNNPAVFYMPSIGMDLTTIFASNTRLAPIQAVALGHPATTHSDFIEYVIVEDDYVGS
                     ESCFSEQLLRLPKDALPYVPSALAPQNVVYNLRENPEVIHIGIASTTMKLNPYFLEAL
                     KAIRDRAKVKTHFHFALGQSSGITHPYVERFIKSYLGNDATAHPHSPYDEYLNILHNC
                     DMMLNPFPFGNTNGIIDMVTLGLVGVCKTGPEVHEHIDEGLFKRLGLSNWLITQTAEE
                     YVTQAIRLAENHEERLAIRRDIIENNKLQTLFSGDPRPMGQIFLAKVQAWLADKNSKN
                     AEVEVKTKKVRKAATASQSAKKQTTSKTQTAKAEKDNTAKTETKS"
     gene            complement(18400..19737)
                     /gene="rimO"
                     /locus_tag="PXB27_000916"
     CDS             complement(18400..19737)
                     /gene="rimO"
                     /locus_tag="PXB27_000916"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HDL5345729.1"
                     /translation="MSASPNIGFISLGCPKNLVDSERILTELRADGYNIIPSYENADL
                     VIVNTCGFIDSAVQESLESIGEALEANGKVIVTGCLGAKENQIREVHPKVLEITGPHS
                     YEAVMNHVHKYVPKPEFNPYTSLVPKQGIKLTPKHYAYLKISEGCDHRCTFCIIPSMR
                     GDLASRSIVQVLDEAKRLVDAGVKELLIVSQDTSAYSLDRKKEEATKTVFWNGMPIKS
                     NLITLCEQLGSMGVWVRLHYVYPYPHVDDLIPLMAAGKILPYLDIPLQHASPKILKAM
                     KRPGSIERTLERIKKWREICPELTLRSTFIVGFPGETEEDFQLLLDFLKEAQLDRVGC
                     FKFSPVEGAVATDMPDQVPEEVKEERFHRFMQLQQEISAARLQAKIGKTVQVIIDDID
                     EEGIIGRSMAYAPEIDGVVYVDNPSNQLVTVGQIISVTITHADEYDLWGILNN"
BASE COUNT         5805 a         4071 c         4482 g         5505 t
ORIGIN      
        1 taaaacaaaa acccccgagc aagtcgaaca aaaagggggg aaaagcaaag gtgcaaatac
       61 agcattatgg ctggttgcga ttgtattttt agcagttgct gcggtaggta atgcctattt
      121 ttcatctcat ttttcattaa ttgttcgagt gcttttattg gtggtgttgc tagttggtgc
      181 tgttgcacta gcggcaatta caaatcaggg gcaaaaggca attcatttta ttaaagagtc
      241 tcgaaccgaa ctacgcaaaa ttatctggcc gacacgccct gaagcaacac aaaccacctt
      301 gattgtattg gcaatgtgcg tagtagtttc cttggtttta tggggtattg actcaatcat
      361 cgtcgcatta attacatttg taacaagttt aaggttctaa aatgagtgaa gtagaagtga
      421 aagaaaatgg ctcaaaacgc tggtatgtat tacaagcgtt ttcaggcttt gaaaaccgtg
      481 tggcagtaac attgcgtgaa tacattaagc ttcacaaaat ggaagagcag tttggtgaag
      541 tattagtgcc aaccgaagaa gtcgttgaaa atgtgggcgg taaacgccgt aaaaccgagc
      601 gtaaattctt cccaggttat gtattggttg aaatggaaat gaatgacgat acttggcatt
      661 tagtgaaaag tgtgcctcgt gtaatgggct ttattggtgg tacggcagat cgccccgcac
      721 caattaccaa acgtgaagca gaccgcattc taaaccgtgt acaagaaaca gcggaaaaac
      781 cacgccacag aaaagaattc caaccgggcg aaagcatttt ggttacagaa ggtccgtttg
      841 cagatttctc cggtacggtc gaagaagtgg attacgaaaa aggtcgctta aaagtatcgg
      901 tgtcgatttt tggtcgtgcg acaccggtag aattagaatt cagtcaagtg gaaaagcaga
      961 gctaatttca attaatttca cacacaagcg gttatttttc tcaatttttt gcaaaattca
     1021 gcggaaaaaa gaccgcttgt aacaagattt attcaacaaa gcgaaagttt tgcttgaaaa
     1081 atagtcgcaa attctttaaa atttgcgacc tattacgagc cagcctgctg actcgtattt
     1141 tttatgtaac aggggagctg attttcagcg ttatcaccca tttagaggaa aattaaaatg
     1201 gcaaaaaaag tccaagccta cgttaaactg caagttgcag cgggtatggc taacccatca
     1261 ccaccggttg gtcctgcatt aggtcaacaa ggtgttaaca ttatggaatt ctgtaaagca
     1321 ttcaacgctc gtactgagag cttagagaaa ggtttaccga ttccggttgt tatcacagtt
     1381 tacgctgata aatctttcac attcgttacc aaaactccac cggcggcagt gctactgaaa
     1441 aaagcggtag gtatcaaatc tggttctggt aaaccgaaca aagataaagt gggtacagta
     1501 actcaagagc aaatccgtca aatcgctgaa actaaagcag cagatatgac tggtgcgact
     1561 atcgaaacca aaatgaaatc aatcgctggt acagctcgct caatgggctt aatcgtagag
     1621 gaataataca atggctaaat tgactaaaaa aatgaaagca attaaagctg gcgtagattc
     1681 tactaaagct tatgacatca acgaagcagt tgaagtgtta aaaaaattcg caaccgctaa
     1741 attcgttgag agcgtggacg ttgctgtaaa cttaggtatc gattctcgta aatcagacca
     1801 aaacgtgcgt ggtgcaaccg tattaccaca cggtacaggc cgtaccgctc gtgttgctgt
     1861 gtttacacaa ggtgcaaacg cagaagcagc aaaagcagcg ggtgctgact tagtgggtat
     1921 ggaagatctt gcagagcaaa tcaagaaagg cgaaatgaac tttgatgttg ttatcgcttc
     1981 tccagatgca atgcgtgttg taggtcaatt aggtcaagta ttaggtccac gcggcttaat
     2041 gccaaacccg aaagtaggta cagtaacacc aaacgttgca gaagcggtta aaaatgcgaa
     2101 atctggtcag atccgctacc gtaacgacaa aaacggtatt atccacacta ctatcggtaa
     2161 agcagacttc tcagctgaac aattaaaaga taacttaaac gcattgttag ctgctttaac
     2221 aaaagcaaaa ccaacaacag caaaaggtat cttcatcaag aaagtaagca tctctactac
     2281 aatgggtgct ggtgttgcgg ttgatcaagc atcactttaa tttttaatag aatcaaaaga
     2341 ccgtagtgtt aatactctac ggtctttttc tttaggtacg aatgttaaga ttattatctt
     2401 tttgctttat ttttttgctt agtgcttgta ctgtattacc tcaaattaag caattaccgc
     2461 cacaggttga atctgtaaaa tcctttaaag ttgaaaataa tggccagcaa agtttgcttc
     2521 taattcaatt ttatgaaaat aaatggcgtt ggattcaaac tgatccttta ggtgctccta
     2581 ttgccagagt atggcttact aaaaaaggtt gggagccaga tggctttgta atgccaaata
     2641 agcaggcaca gctattattt acagctttag tcaatgcatt aaaccctcat tctccacctt
     2701 tttatttaga taaaggttgg aaaattgaga gggaaaaatc ccaatttacg atttggctac
     2761 cagatagcag taaatggaaa gtggaaatat tgggggaata agcgaaaact tgaacctcta
     2821 tgcttgaatc acttgaatat gaattttata attttagtgc cattacacaa taatactgga
     2881 ataaataata atgacactat atttatcaga gcccgccatc accagtccga ttggatatgg
     2941 catcgtggag catttgaatg ttttaattaa tgcgaaagta tctcctttag atacgacttc
     3001 acagccttat ttacgacata atatttcaaa tagttcaccg aaatttctag ggcaagtctc
     3061 tgctagattg cgtgattttc ctgttgaact atctattgaa caccgcagcc gcaataacca
     3121 actgctatgg cacgcccttg cagaaattga gccgaaaatt accgcttgta tcgagcaatt
     3181 cgggcgtgag cggattgcgg tgatcatcgg cacgtccact accggcgccg atgaaaatat
     3241 tcccgtcttc aaagccggtg tgcagggcga ttatgccacc gagcagttta atcaacagca
     3301 aattctcttc tctgcgccgt ctgatttcgt tgccgaagtg tatggcttaa cggggctgag
     3361 ttacggcatt tccaccgcct gcacctcaga ggcaagagcg ttaatttctg ctgcccgttt
     3421 actgaaagcc ggcttgtgcg atgcggtgat ttgcggtggt gtggataccc tttcgccttt
     3481 aactatcagc ggatttagct cgctggaagt gttatcaaac caacaaacca acccattttc
     3541 cgctaaccgt aacggcatta acatcggcga aggcgcagcg gtgtttgtga tgagccgtga
     3601 accactggac gaacatcgca ttcagttact gggttatggt tccagctcgg acgcttacca
     3661 tatgtcctca cctcaccccg aaggggaagg ggcaatttcc gctttccaaa atgccctgca
     3721 caatgctgat ttagacgcag ccgacatcgg ctggatcaac ctgcacggca cagggacgct
     3781 tcataacgat caaatggaaa gcctcgctgt ggcaaaaatc ttcggtaatc agaccgcttg
     3841 caccaccacc aaaccctaca ccgggcatac ccttgggctg ccggtgcggt ggaagcggca
     3901 attttgtggg gaatgctctg tcgccaacac aacccgaccg gcaagctccc tgcgcaatta
     3961 tgggataaac aagcggacga aaacctgccg aacattgcga tcaccgatga acagagccgt
     4021 tgggcagcag gcagacggat tggcgcaagc agctcttttg cctttggcgg caataacagc
     4081 gtactgattt tgggagaaag cgatgcataa attcaccttc cctatcgaac aaattgcacc
     4141 attggttcct cacagtggca atatgatcct actcgaccgc attaccgcat tcggcgagga
     4201 ttttttgacc gctgaaacca atattcgccc tgacaatccg ctgatcaaac acggaaaact
     4261 tgccacctac gccagcattg aaattatggc acaagccggc gaaccagttc gcttaggcta
     4321 cctgctcggc actcgcaaac tgcacctcca ccgccaagaa atcccgattg gctcacagct
     4381 acaaattcag atcaaaatgt ctattcaaga tgccaccggc ttcggcgtat tcgactgcca
     4441 actgatcgat ttagccgaca accaagtgct tttagaaggg gcgctgaatg tgtttagtcc
     4501 gaaatagagt tggtagtagt aagtagtagt aagtagtagt aagtagtagt tggtagtagt
     4561 tggtagtagt tggtagtagt tggtagtagt tggtaggaaa ttcgttatgc aagaggccta
     4621 tgacattcaa catctttgta ctcgcaaaga agataaatcc tttgatcgca aaagtgcgag
     4681 aaaagaaccg aaagagattt tgcggcattt gattggtttt gcgaatgcag acggcggttt
     4741 attagttatt ggtattgaag ataatggcga aattaccggt tttaactacc aaaaagccca
     4801 tcagccggaa gaatttatcc acgctctgca caatttacaa aaaatgccga taccggtgac
     4861 ctatcagcaa attgaggtgg taaatagtcg aggtttagcg gattttgtct tattatttga
     4921 agttacagct tcgagtggga gggtgattac ctctcacgat gatcgggttt ttctacgctc
     4981 aaaagatcaa actttggaac tcaattttga acaacgtttg caattagagt acgaaaaagg
     5041 gcagcgtgct tttgaagagc aaccgatagc acgcgctgat tttactgatc tggatatgga
     5101 tttattagaa cagtatcgac agaaattaca cacagatcgc tcaatagaag agattctgaa
     5161 agcccgagat ttaattaaca aagaagggca aattacgaat gcctgtattt tgctatttgg
     5221 gaataacccg acacgttatt ttcctaatgc ccgtttgcgt tttttgcggt atgaaggtaa
     5281 agcacctctg accggtagaa cttttaatgt cgtcaaagaa tttatgcttg aagagcctat
     5341 tcctaagtta ttggagagtg ctaaggcagt attaaaaagc caattacgag agttccaacg
     5401 tttagatgaa aacgggcaat tcacaaaaat tgatgagtat ccagaatttg cttggttaga
     5461 aggtattgtc aatgcgattg cacaccgtag ttacagtaat caaggggact gcattcgcat
     5521 ttcgatgttt gatgaccgtt tggaaatttt tagtccgggt ggattacctc gccctgtgac
     5581 gcttgaaaat atgtactata cccgtttttc acgcaatccg aagattgcac gagtgctgta
     5641 tgaatttggt tgggtcaaag agctgaatga aggtgttaat cgtatggcgg aggaaatgaa
     5701 cgcttaccaa cttaatacac cttcttactt tgaacccaat gaaaatgcgg tcagactgat
     5761 tttagaaaat aatattgaac atcggacatt aagggcatta gaacgaatag aagaaatttt
     5821 tacgcaggat attatggcga cattaaataa aaatgaagtt actatcctgc aatatttata
     5881 tgaaaatgaa atgattactg taaagattgc tagtgagata ttagatcgga gtaaagaatt
     5941 atctcgtaga atattaaaaa gtttagaaca aaaaaatatt ctcacttggc acggcttaag
     6001 tccaaaagat ccaacccaat actacacact caagaaatag taggaaatat aagatgaata
     6061 atacagttct catcaccggc tcaagccgtg gtatcggtaa agcgattgct ttaacccttg
     6121 cacaacaagg gtttgatatt gtggtacatt gccgttctcg tgtggaagaa gcggaagcgg
     6181 tggcacaagc ggtcagaaat ctcggaaaaa atgccagagt gttacagttt gatgtggcgg
     6241 atcgtgaaaa agtgcggtcg attttagagg cagatgtgga acaacatggt gcctattacg
     6301 gcgtggtgct aaatgcaggg cttacccgtg ataacgcctt tcctgcttta accgatgagg
     6361 attgggatag cgtgttgcgt accaatttag acggttttta taatgtcctg cacccgatta
     6421 tgatgccgat gatccgtcgc cgtaaagcag gcaggattgt ctgtatcacc tcggtttccg
     6481 gcttgatcgg caaccgtggg caggtcaatt acagcgcttc aaaagccggc attattggtg
     6541 cggcaaaagc actcgccatc gagcttgcca aacgtaaaat cacggtaaac tgcgtggcgc
     6601 ccggcttgat tgataccgat attctggacg aaaacgtccc aatcgaagaa attctcaaaa
     6661 tgatccccgc aggacgaatg ggcgatcctg atgaagtggc acacgccgtt gcgtttttaa
     6721 tggatgaaaa agccacttac atcacacgcc aagtcatcgc tgtgaatggt gggttgtgtt
     6781 aactttagga taataattcg taggggcgga tccagtaccc gcccgaagcc aaaaagagat
     6841 aaatatgaaa cgagtcgtca tcaccggaat tggtgtggtt accgcttttg gtaaagagtg
     6901 ggcggaaatt cacggctatc aaccgccgaa acattggaac cgcaagcagt tgcgcagcct
     6961 tgggcgagtg tcgcaatttg tggtggaggc cagcgagcgg gctttagcac aggccggttt
     7021 gttggacgaa aacggcgtga ttttcgactg gctaaaagac aggcgaatgg gcgtggcgtg
     7081 tggctcgtcc acaggctcta ccaatgacat caaagatgcc gccgaattgc tgatgaccgg
     7141 caaatcggac ggtttcaatg ccaacaccta tgtgcgaatg atgccccaca ccaccgctgc
     7201 caatattggc attttcttcg ggctgacagg gcggattatc ccgacttcca gcgcttgctc
     7261 ctcgggcagc caaggtatcg gttacgctta cgaagccatt aaatacggta tgatcccgat
     7321 gatgcttgcc ggcggtggag aagaattttg tgcctccaaa gtctatgtct tcgacagcct
     7381 ctatgctgcc agccgccgca acgacaaccc aaaaggcacg ccacgcccct atgacaaaga
     7441 ccgagacggc ttagtgatcg gcgagggagc gggaatgttc gtattggaag agctggaaca
     7501 cgcgttagca cgaggggcaa acattatcgc cgaagtggtg ggctacggtg caaacagtga
     7561 cggttcccac gtcacccgcc cacaagccac cacaatgcaa cgctgtatgg aacttgccct
     7621 taaagatgcc ggtattcaac cgaaccaagt cggctatgta aacggacacg gcacggccac
     7681 cgagcagggc gacattgccg aaactcaagc caccacagcg gtgttcggca aaattccgct
     7741 cagctcgcaa aaaagctact tagggcatac gctcggtgcg tgtggtgcat tggaaagctg
     7801 gttcagcatt gaaatgatgc gagacggctg gttcgcccca acactgaatt tagaaaatgt
     7861 cgatgaacgc tgtggcgact tggattacct tcaaggtgac ggacgggaaa tccacaccga
     7921 ttatgtgatg aacaacaatt tcgccttcgg cggtgtgaat acctcattga tttttaaacg
     7981 ttggaaataa ccgttttcaa ataatgaaaa agatattatt agtttattac tcccaaaccg
     8041 ggcaactcac ccatttggca gaaaattttg tacaatcgct tgaacaagcg ggcgtttttg
     8101 tggaaaaact cgcaatcaaa ccgcaacagg aatacccgtt tccgtggcgg tttatgcgtt
     8161 tttttaacac cttccccgaa accgtccacc tcacaccgcc gccgattgag cctctgccct
     8221 ttcagcacga aatatacgat ttggtgatca tcgcctattc cgtgtggttt ctctccccca
     8281 gccagccgat taccgctttt ttacagagtg aacaggcaaa aaagtgttga aggatacgcc
     8341 cgtcatcacg ctgatcggct gtcgcaatat gtggctgatg gcgcaggaaa aagtgaaaaa
     8401 actgcttgcc gagagtgagg cgaagctcat tggcaacgtg gtcaaagtgg atcaatctaa
     8461 cgactgggcg agttttatca ccacaccgct ttggatgctg accggcaaga aaaaaaatcg
     8521 gcaagctgcc gagcgctggc attgccgaaa gcgaaatgga agatgtaaaa cgcttcgggg
     8581 caaaactttt gcacattttt agccaaaacc aaccgcttga cagcaccctt ttccgccaaa
     8641 tgcaagcggt caaaattgac gaaaaattga tgatgagcga aaaagtcggt gcgagaagtt
     8701 tccacctttg gggcaaatta ctgatcaagt gcggtcaaat ttcaccgcac ttacgccaag
     8761 cggtactcta tttttacatc ctctttttag tgacgttaat tttaaccgtc gtcccgattt
     8821 caacggtgat taaacggcta ttacgcccac tgctgaaaga aaaattagaa aaacaaaaac
     8881 ggtattttgc cgagccttcg ggcgagtaaa cgtaccctac acggaaactt tatgctatct
     8941 aacgtctata tcaccaacac ctccgccttt ttacccaacg cccctgtgtc caacgatgca
     9001 atggaagcgg tgttgggaat ggtgggggaa acgccttccc gagtgcggaa gatgattttg
     9061 cgttctaacg gtattcaaca tcgttattat gccattgacc cgataacccg ccaagcgact
     9121 cacagcagta ccgagttggc ggtgcaagcg gtcaaaaaat tgggaaattc tgcaacatca
     9181 gcgacttgct tggcggtggg gacatcttac cccgaccaaa ttatgccggg gcagggggtg
     9241 atggtacacg gcttgcttga tgccgaaccg atggaagtgc tgaccactgc cggtgtgtgc
     9301 gtggcgggaa tggcagcgat gaagcacgcc tataatgccg tacgcatcgg tgagcatcgt
     9361 gctgcgattg caacggcttc cgaaaccgcc tccgccatta tgcgcagtga acatttccgt
     9421 gccgaaatcg atcataaaaa actggacgat gctcgccctg agatcggttt tgagaaagat
     9481 ttcctgcgtt ggatgctctc agacggtgca ggggcggtat tgcttgccaa tcagccgaac
     9541 gaaaaagggt tgagcctcaa aattcactgg atcgatctgg tctcttacgc taacgaaatg
     9601 ccggtttgta tgtacgccgg tgcggaattt attgacggca actacaccgg ctggaaagca
     9661 atgagcgaag cccaacgtgc cgaatgctcg gcaatggcgg tgaaacaaga tgtcaaactg
     9721 ctcaacgaaa acatcgtcaa atacacggtg gaaaaagcat tggagcggat tatcccgaaa
     9781 tacaagctca acgctgaaga gattgactac ttcctgccgc actactcgtc cggttccttc
     9841 cgagataaat tattggaagg tttacagcgg atcaatttcc tgatcccaca ggaaaaatgg
     9901 ttcaccaacc tgactaccaa aggcaacact gggtcagcct cgatctacat tatgctcgat
     9961 gaatttatcc gcacccacga tctcaaacag ggacaaaaaa tcctctgcta tgtgccggaa
    10021 agcggacgtt tttcgtcttg ttttatgctt ttggaagcgg tggtaaattc gtaaaatcgt
    10081 agggacgggg gctattcccg tccaacaatc atcggaaaac gtatggacat caatgacatt
    10141 ccccaagaca acagcaaaat cttcaacggc gaacgcaaag tgatctacgc ccgcaatccg
    10201 caaccagaaa tccgccaaat gatctcctct attctcgctt gctacgcttg ggacgaaaac
    10261 aacccgtttg tagcgaagtc tgatgcacgg ttgaaggcat tggcggaagt tgttggtaaa
    10321 gcagaaattg gcgattaagt ttgattttgt gctttacagg gtcgcagatt atctgtataa
    10381 tttgcgacct taacttgcga gagcaagctg atggtgctta gcctatctaa gccccgtcta
    10441 agactgtagg ggtgaaaact taataatcct acatagatgg tgaacagaca gaattctctg
    10501 cttctggaca ccttaggctc aaagaagatt gcagtttgta gcaatgcaag cggtaatatt
    10561 tttgggattt tttgtaaatt ctgcttgaaa aggcagagtg tatcaggagc taaaaccaat
    10621 ggcattaaat cttcaagaca aacaagcgat tgttgctgaa gtaaacgaag ctgccaaagg
    10681 tgcactttca gctgttgttg cggattctcg cggtgtaaca gttgaaaaaa tgactgagtt
    10741 acgtaaagca gctcgtgaag ctggtgtgac aatgcaagtt gtacgtaata ctttattacg
    10801 tcgtgcagtg gaaggcaccg agttcgagtg cttaacagat acgtttaccg gtccaactct
    10861 tatcgcattc tcaaatgaac acccaggtgc agcagcacgt ttattcactg aatttgcgaa
    10921 aacaaacaaa gagtttgaac ttaaaggtgc agcctttgaa ggtaaaaaac aagatgttgc
    10981 attcttagca accttaccaa cttacgaaga agcaattgca cgtttaatgg gcacaatgaa
    11041 agaagctgcg gcaggcaaac ttgttcgcac tcttgcagca ttacgcgatc aaatggaagc
    11101 agcagcttaa ttttaagcac tcttctttat tcgtttaaca acttatttta ctttaggaat
    11161 cgattgttat gtcattaact aacgaacaaa tcattgaagc gattgcttct aaatcagtat
    11221 cagaaatcgt tgaattaatc actgcgatgg aagaaaaatt cggtgtttca gcagcggcag
    11281 cagtagcagc agctccagca gcaggcgcag cggcagcaga agagaaaact gagtttgatg
    11341 tagtattagc agaagctggt gcaaacaaag ttgctgtaat caaagcagta cgtggtgcaa
    11401 caggtttagg cttaaaagaa gctaaagact tagtagaatc agctccagct gcattaaaag
    11461 aaggcatctc taaagcagaa gctgaagcac ttaagaaaga attagaagaa gctggcgcga
    11521 aagtagaaat caaataattt ttgatttatt ttgtcagaac ttcaagttca acaaaccccg
    11581 atggaaacat cggggttttg ttttttcaga attgtgtaat gtagatatga ttacctttat
    11641 ccattacttg cgcagcagtg aaaaatgtaa aaattccaca aagtgatctg cccagtgcat
    11701 ttcgtggtgg atttcatttg ccataatccg caggcggata ttgtctaacg gcacgccaat
    11761 ccgaatcagt gcttggtagt aattgagcga acaatcaata taggtttggt tcgtattaga
    11821 gataaagtgc gaatcgattt catcgccttc gttagtgcca acctgaataa acactttggt
    11881 attgggttgt aacggatatt ggtgcgtaaa acgcagaaaa tccggctcac taaaccaaga
    11941 ggcaagcgaa aacaccccta aatgcccgaa aatatgtggg tagcggctgc ccatataagc
    12001 ggtgataatg ccacccattg aactgcctgc caataaggta tgttcttgct gaggcttggt
    12061 gcgatagtga atatcaataa agggtttaac cgtttccacc acccatttgg catattcctc
    12121 ccctaagcct ccggcatttc gggtttcagg ggttgagccg gtggttgttt tccacgggcc
    12181 gtattcattt aagcgattag ctcctgcgtt atcaatgccg acaataatca tcttcggtaa
    12241 ttcttggtgt gatttaatgg ttggaataat tttccaagaa tgccccgaat aagactcttt
    12301 gctgtaaaac acattttgcc cgtcgtgcat atataacacc ggataacgtt cccaaccttc
    12361 gttgtgataa tccttcggca atagtacccg aatgcggcgg tgatggtgta aatagggaac
    12421 atataaaaaa tgctcctgca tttctaaata gtggttaagt ggtttttgta ccatcagtct
    12481 gtccttttgc ttaaattgtg gcgtatttta caccttaatc agtctgagtt aaaacaagcg
    12541 gtcagaaaaa gtcaaaaatt tgcaaattgc tgttacctaa cgcaatcggt tgccttatgc
    12601 ttttgaggaa tataaaagag aaactgtgat ctaagtcaca gcaattttta ttcggtttga
    12661 ctaaaatccg aactatcact taatagagag gaaaatgtta tgcaggcttc attacaagat
    12721 attcttgagg tagtaaaagc aaaaaatctc acttatcagc aaaaattgat gagtttaggc
    12781 aatattgctg agcggttatt taacccgatt gaactacttg gctacactga agaagagtgg
    12841 gaacacattg aaaatcagat gatttgcgat ttaaatgaag gttatgcgat ttatcgccca
    12901 cgttatattt tgcctgatta taatgtgtat atgcaaaaag gttgccaatt cttagattta
    12961 cctccgccaa ccaatttaga tgaagcctta gacggcttgt taattctgta ttcacacgtt
    13021 ccgtccatta ctacttttcc ggtgtatatc gggcgtttag accaactgtt agaaccgttt
    13081 ataaccgatg aagagcaaga ttacattaaa atcaagcgtt tcttaaatca tatcgataaa
    13141 accatttccg attctttctg ccacgctaac atcgggccga atgacaccaa agccggtcgt
    13201 ttaatcttaa aagcggtgat tgagcttgaa aacattacac cgaatatgac gattcgttat
    13261 gataaaacca aaacatcgcg tgaatttgcc gaactggcgg cgaaagcgtg cttgttggtg
    13321 tccaaaccgt cttttgctaa tgatgcctac tacattcaag atttaggcga agaatacgga
    13381 attgcaagct gttataacgc cttaccggaa tgtggcggtg cttacactct gacccgcctg
    13441 cgtttaggta ctatcgggcg tgcttgtgcg agtgttgaac aaatggtcaa tgagatgctg
    13501 ccgaaagtgg caaaactcgc cctttccacg atggataaac ggcataaatt cttggtggaa
    13561 gaaagcaatt tctttgaaag tagcttcttg gttcaggagg gctttatcaa acgtactaac
    13621 ttcacaagta tgattgcgat tgtaggtctt gctgatgcgg tgaatcattt actcaagcaa
    13681 gaaggtttaa atgaaacctt tgggcaaagc aaacgtggcg atgaaattgc gacgctgatt
    13741 atggataagc tccaagcagt aaatgacgct cataaagggc tgtatgttga acgaaccaat
    13801 aaccaatatc tgttacacgc tcaagtgggg gcaaataacc acgctgaaga caaaatgaac
    13861 acgccggcac accgcattcg tgtgggcgaa gagccaacct tattggcaca cttaaaacag
    13921 tctgctcctt tccataaata tttcccatcc ggtacggggg atttattcgc ctttgaccaa
    13981 acctacactg atcatttaga tgcggtggtg gatattattg acggctcgtt cgcccaaggt
    14041 tatcgctaca ttaccactta cctgaaaaat actgatttga ttcgagtgac aggctacttg
    14101 gtgaaaaaaa gcgaggttga acgttatcgc aaaggcgaag cggtattgcg tgataccact
    14161 tggtatggtt caggcaccga tgattgtgcg aaagtgttcg accgccagct gcgtgatgaa
    14221 gaaaatgtga atgcagggta acaatggctg cactttctga gatttttgtg ccgttacacc
    14281 gcattatccc cttctcgaat gtagaagggc agggcaatcg taccagcatt tttctacaag
    14341 gttgcaagct gaattgcctg tattgccata acccggaaac cattccacgt tatagcaagg
    14401 aagcaaaaca ggttagcttg caatacttgt acgatcaagt gatggaggca gttcccttta
    14461 ttcgaggagt cactatttcg ggtggagagc caaccattca tcataaaaaa ttagtgccac
    14521 tgttcgaggc gttgcgtgag caagggctaa cctgttattt agacagcagc ggcttttttg
    14581 attttgaggc aacagagccg ttaattaatg ttaccgataa atttctgttc gatctcaaag
    14641 gagatggggc gggcttacaa acgctatgct ttgatcggaa aaaccaagcc ggcaaagtgc
    14701 ctgaacaggc tcttccaaac gtgaatcata ttaaacctga aaacttggct cgcaatttgc
    14761 aaaatttagc caaattatta ccgcttgata aggtggaaga agtacgtttg gtttacctca
    14821 atgagtttta taacgcccac catttgctcg aaaaagtggc tgacctactt aaaccttacc
    14881 cagatgtact acttaaaatt attcgggtac acaccaaagg agcgagagat gaaacaggct
    14941 tggctcgttt tgtacccact atcgagcaaa ccgatgaact ggcagcattt gcaaaacggc
    15001 acggtattca aaaagttatt actatttact aatggagact tacgatggat atactaatta
    15061 gcataattgg tgtttttgtg ttattaggct taggtgtttt attatcaaat aatcgcaaag
    15121 ccattaaatt tcgcacaatt ttgggcgctt tggcgattca aattggtttt gccgctctta
    15181 ttctttactt tccagcggga agaaatgcat tgctagctac tgcaaattgt gtgagcaata
    15241 ttattaatta tggtaacgaa ggtattagct ttgtgtttgg caatttagcg aatccgagta
    15301 atagcagcat tggttttgtg tttgcggtta aagtattgcc tattattatc ttcttctctg
    15361 cgttaatttc aatgctttac taccttggtg tgatgcaatg ggtgattaaa attattggtg
    15421 gagcattaca aaaaacacta ggcacttcca aggcagaatc aatgtcagcg gcagctaatt
    15481 atttttgttg gtcagacaga agctcctcta gttgtaaaac catacattag ccgtatgaca
    15541 gagtcagaat tatttgccat tatggttggt ggaactgctt caattgctgg ttccgttatg
    15601 gctggttatg ccggaatggg cgttccttta acttatctta ttgcggcctc ctttatggct
    15661 gcaccagctg ggttactatt tgctaaatta atgtatcctc aaacggaaac cttggagagt
    15721 aaagcagatg acaatgttga agttgaaaag ccaaataacg tggttgaggc cctagctaat
    15781 ggtgctagtg caggtgttac tttggctatt aatgtcggtg ctatgttaat agcatttatt
    15841 gcagtgatcg ctttattaaa tggctttatt ggtggcattg gtaattggtt tggttatgag
    15901 gggctaacct tacaaatttt attaggttat ttattccaac cacttgcttt cttaatcggt
    15961 gtgccgtggc aagatgccga tattgcaggt caaatgatag gtatgaaact tgctgttaat
    16021 gaatttgtcg gatatcttga gtttgctaaa tatctacagc ctgatgctgt ggtgcaatta
    16081 agcgataaaa ctaaggcaat aattactttt gctttatgtg gttttgcaaa ctttagagca
    16141 attgcggtat tgattagtgg tatcggaagt atggcaccaa gtcggcgttc agatattgct
    16201 cgtttaggtt taaaagcagt gattggcggc acattggcga acttaatgag tgcaactata
    16261 gcgggtttct ttattggttt aagtggtgcc acattaggtt gatacaagcg gttaaattga
    16321 ctcacttttt tgcaaaaagc ccaagtaagt taaatgacta cttgggcttt gtttaatgaa
    16381 ctagctctta gtttcggttt ttgctgtgtt atccttttcc gctttggctg tctgtgtttt
    16441 cgaagttgtc tgtttttttg cggattggct agcagtagct gcttttctta ctttctttgt
    16501 tttaacttct acttccgcat ttttagagtt tttatccgct aaccatgcct gcactttagc
    16561 taagaatatt tgtcccatcg ggcgaggatc gccagaaaat aatgtttgta atttattatt
    16621 ttcaataatg tcgcgtctaa ttgctaagcg ttcttcgtgg ttttccgcta aacgaatagc
    16681 ttgagtaacg tattcctctg ctgtttgggt aattaaccaa ttagatagcc cgaggcgctt
    16741 aaataaacct tcatcaatat gttcatgaac ttcagggcct gttttacaga caccaactaa
    16801 accaagcgta accatatcga taatgccgtt tgtatttcca aatgggaatg gatttagcat
    16861 catatcgcaa ttatgtagaa tatttagata ttcatcatat ggcgaatgtg ggtgtgcggt
    16921 tgcatcattt cctaaatagc ttttgataaa acgttccaca taaggatgcg taattccact
    16981 agattgccct aatgcaaaat ggaaatgagt ttttaccttc gccctatcac gaatggcttt
    17041 taatgcctct aagaaatatg ggtttaattt catagtggtt gatgcaattc cgatatggat
    17101 aacctctgga ttttcacgta ggttatagac cacattttga ggcgctaatg cggaaggcac
    17161 atagggtagc gcatcttttg gaagacgtaa taattgttca ctaaagcagc tttcggaacc
    17221 aacatagtca tcttctacga tgacatattc aataaaatca gagtgtgtag ttgccggatg
    17281 acctagcgca acagcttgaa ttggtgctag gcgagtgtta ctagcgaaaa tagtggttaa
    17341 atccattcca atgctaggca tatagaatac ggctggatta tttttatcac agatatcttt
    17401 taaaaagctt aagcggtcaa atagtgagcc atcttgtggt aataaatgaa attcgtcgaa
    17461 tacctgttgc cccatttcat ctacagcatc actgcctagc ccaataagat ggaaatgttc
    17521 gcgtgcagca accattgagg ttgaatgagt acgataaata gaatgtgatg aatggaaatg
    17581 ctccaataat actaccatga caggtttatt atttctggta ccaagctgtt caatttttct
    17641 atcttcccaa ccgacagaaa gtaaatgacg acgaattaca gcatttaacg accgttttac
    17701 attatgttta tttgcttcaa tatcataact gcaatgcata taaacatcgt gtgaaatggc
    17761 gctcggtagc ttgttcaagt tttcaatttg cgctaatttt tctgggaacc attgcaaaat
    17821 agccgcgcgt tttgcaaaag cagaggatgt accaataaat ctaggtgatt ggagtgcaaa
    17881 acaaagtgat gcggtgagat ctgatgaagc attccatgca gcatctagat tcaataaaat
    17941 attagattcc ggtaagtata aaatacagaa cttaattagt gcttgtaatg tggcatctaa
    18001 atcaaccgta tttggattag cagattcttt atttctattg taagtacgca agatatggtc
    18061 tgcgtttacg tatggagaac tggcaaaaat taatgctatc cagcgttgta atgtcagaaa
    18121 acgcatagca ccgtgttcgg agatctctaa tgcaggatct tcaaacaagc gagtaatcgc
    18181 taccgccatt cgagtacaga aatagatagc tttatcattt tctaaatttt caatctgctc
    18241 tggcatattc aattcaatat tggaaatgcc accaaaatta ctatctaatt ttgaaaggat
    18301 agacagtagc tcggtacaag ccgattcata atcttttttc gcgaccgcct gctcaaaacg
    18361 gatgacgcta ggcatatttt ctgctgacat aatgtatcct tagttgttta aaattcccca
    18421 cagatcatac tcatctgcat gtgtaatggt tactgaaata atttgcccta ctgtcactaa
    18481 ttggtttgaa ggattatcta catagactac accgtcaatt tccggtgcat atgccattga
    18541 gcgaccgata atgccttctt cgtcaatgtc atcaataatt acttgtacgg ttttgccgat
    18601 ttttgcttgt aagcgagcag cggaaatttc ttgttgtagt tgcataaaac ggtggaaacg
    18661 ttcctctttt acttcttccg gaacttgatc cggcatatcg gtagcaacag caccttccac
    18721 tgggctgaat ttaaagcaac caacacgatc taattgtgct tcttttaaga aatcgagtaa
    18781 tagttggaaa tcttcttctg tttcccccgg gaagccgaca ataaaggtag aacgcagagt
    18841 taattcaggg caaatttcac gccatttttt aatgcgttct aaggttcttt cgattgaacc
    18901 cgggcgtttc atcgctttta agattttcgg gctggcgtgt tggagtggaa tatccaaata
    18961 cggcaaaatt ttgccggcag ccataagcgg aattaaatca tctacgtgtg ggtatgggta
    19021 aacatagtgc aaacgcaccc acacgcccat tgagcctaat tgctcacata gcgtaattaa
    19081 attactttta atcggcatac cgttccagaa aacggttttg gttgcttctt cttttttgcg
    19141 atctaacgaa taggctgagg tgtcttgtga gacgattaac agctctttta ccccggcatc
    19201 aactaggcgt tttgcttcat ctaatacttg cacgatagaa cggcttgcta aatcgccacg
    19261 cattgaagga ataatgcaga aagtgcagcg atgatcgcag ccttcggaaa ttttcaaata
    19321 agcgtaatgc ttcggtgtta atttaatccc ttgttttggc actaagctgg tgtaagggtt
    19381 aaattccggt tttggcacat atttatggac gtggttcatt accgcctcat agctatgtgg
    19441 ccctgtaatt tccaatactt tagggtgtac ctcacgaatt tggttttctt ttgcccctaa
    19501 acagccggtg acaattactt ttccgttagc ttctaacgct tcaccaatgc tttcaagaga
    19561 ttcttgtact gcggaatcaa taaaaccgca ggtgtttaca atcacaagat ccgcattttc
    19621 ataactaggg atgatgttat aaccatccgc acgcagttcg gtcaaaatac gttcggaatc
    19681 aactaaattt ttagggcagc ctaagctgat aaaaccaata tttggtgaag cactcatata
    19741 actctcaata actaaaaaga aaaatgccta caatttttgt ctgtaggcaa aaaactaaca
    19801 agattctaca aaatcttgcc caaaatagcg ataacaagcg gtcaattttt aataaacatt
    19861 tac
//
LOCUS       DANLUQ010000033        19367 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.033, whole genome shotgun sequence.
ACCESSION   DANLUQ010000033 DANLUQ010000000
VERSION     DANLUQ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 19367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19367
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.033"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(154..1626)
                     /locus_tag="PXB27_000917"
     CDS             complement(154..1626)
                     /locus_tag="PXB27_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HDL5345730.1"
                     /translation="MTFTVDSYLEYFLTLLAWIINNNIFAVLVQTGLFVLPLIFVLIS
                     TWMEVKKQGEDEGNKGDLLITWLSLKFYPAMFVVVLVLAPMIPINLNNIELNVERSKA
                     CGYRVPTAPEDSGYNQMNSELGDQQARVPIWWGLFHQINKGVTHALTASIPCKPDLRQ
                     VRFEVQHEKINNPALLTEVRLFVQQCYVPARQKIQNSQLSLSPAQVRETSWLGGRILI
                     ANSELYPRYRAQQPHSLFPYNASRDNGLPNNGEGGFPNCAEWWDTANNGLKDRLLDDV
                     KQNFSAKVIDFFSSKENSDESILRTVLRAENMDISRGKVYPGYGGNLDRSFTNEVTKT
                     ASMFGLSLGSIGAFPAFDSMRQALPMVHSFALMALIILTPLIMVMSGYSLKTLVTLTF
                     VNFALVTLTFWWELARWLDSWLLSVLYDSGTHSRINPYFLENTQDDIIVNFVMGSMFL
                     VLPAIWLGSMSWVGVKMGEFASHLTTGSRDSKTAGSKIGK"
     gene            complement(1637..1954)
                     /locus_tag="PXB27_000918"
     CDS             complement(1637..1954)
                     /locus_tag="PXB27_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
                     /protein_id="HDL5345731.1"
                     /translation="MKSDTTKPKMTRVITVLKITLMIIGIILLLLVVGWLAMTYFPST
                     NFQAWFYETRYAWLGWRFVLYVAIWLLMVQLNRKGQMPLKARLMILSVIPIIEGLNLL
                     YLL"
     gene            complement(1944..2372)
                     /locus_tag="PXB27_000919"
     CDS             complement(1944..2372)
                     /locus_tag="PXB27_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345732.1"
                     /translation="MNKQHYRLFNAGPQKSSLGVVSPAVETQLLGFCEQIRQLIGVGK
                     QITLFPHRVVQNHWAIELSGEQGSVHLLLNVTGRSLLPTSNNSEWSARLIIEIADHVD
                     AYWFSAFLTQYLGLKSNLLPLSTSIADNSVYNEVIDNNEV"
     gene            complement(2372..5209)
                     /locus_tag="PXB27_000920"
     CDS             complement(2372..5209)
                     /locus_tag="PXB27_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HDL5345733.1"
                     /translation="MSFLSEVFSSFKPTKAGVISSEPNENTGESDIIQNKMSEVNNEV
                     QQLKRSGKLTQSQHHRRLYSQEPSFIDHLPWGEYLNEHGVILLDDARSVGAVFDIKPI
                     GTAGRSIEFLSRIRNLVKDALQDSFDELDVNPYVVQFYCQDSDDLQPYIEQLRNYISP
                     IAKNSAFSEEWLRVQAKHFKDIGKPEGLFIDERVTNTAWSGRIRSTKMVIYRYVDKSN
                     DTNENLIVEQLNNACSRINGALTTAGLQLQRLDGEQFRQWLLRWFNPNPSINFEGLSN
                     NDCYHLFGKQTSNDGLPELAFNDFSEDLFYSQPKNEGNYWYFDDQPHEVVVVNNLRKQ
                     PEVGLMTGEITRGRNINALFDLLPESTIMAITIVIHPQDKLEAHLEKISSRAVGENFE
                     SKYLKEDCQTVQSYLKEGHKLYQAAIAFYVRGKDEKELRTRSRQLRTILLSNNLEPVK
                     ENAEIAPLNSYLRWLPMNFNPQMDAKSHYYTKYYFVQHLANLLPIFGRDTGTGNPGIT
                     YFNRGGAPFNFDPFNRKDRTKNAHKLLLGPTGSGKSATLCGQLCQAMAVYRPRTFVVE
                     AGNSFGLLADYAERFGLTVNRITLAPNSGIVLPLFSEAHRLLDMELNPEDVPEDIEDN
                     DDTDDEGDMQRDLLAEMELTARLMITGGEKKEDEKMSRADRAAIRRAILNAARQTKSE
                     GRQTLTEDIKNALEALSHDEHTREERRTRLAEMAESLEMFCTGVNGQFFNREGAIWPE
                     ADLTLVDLAMFAREGYEAELAISYISLINHINSLGEKYQHSDRMILNVTDESHIITAN
                     PLLAKFLVKGSKMWRKLGIWLWLATQNMDDFPNEAAKLLSMVEWWELLNVSASEIDEI
                     NRFRKLSEAQKTMLLSAKKSDRKYTEGVVLATNMEALFRVVPPSLFLALGMTEKHEKA
                     ERKQLMMQHGYTELDAALEVAARIDKARGIH"
     gene            complement(5212..5610)
                     /locus_tag="PXB27_000921"
     CDS             complement(5212..5610)
                     /locus_tag="PXB27_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HDL5345734.1"
                     /translation="MKHLFCIMISALLITGCTTSTEELLPTNGSTMRDIWDKGASNND
                     LHLYRSQQGRTLDPVDYIPNSEQRAYTRTAENEINNLFPRLPNPDLIMYVYPHQTRSG
                     EPLSIPGYSTVLPFYGRVQYAQPGDRTRGL"
     gene            complement(5623..7077)
                     /locus_tag="PXB27_000922"
     CDS             complement(5623..7077)
                     /locus_tag="PXB27_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HDL5345735.1"
                     /translation="MKANKALFVILGIVFILVGGIVYALFGRSESVPQPTQQEILDIS
                     IKDLTPEQVRTMGIQGDTPIDTLRTLVTTVKSNERRMDELIDENRQLKQKEDLVNGRI
                     NDAVNNETRKLQEQIADLKMQLFDMATLVNNKPTTQESTPQVEAQPNTVPNKGFGTAG
                     DKLPIGGATMSNVDTSGMYWVEPSDQISLDASGKPVSGNTVATKFGFPKDFNGGQSAG
                     NNEKKGLPFFPETDTVTSSQGTAGNARGKNQMTSVYTIPSNSTFLDSVAMTGLIGRIP
                     IDNRVSDAYPFKVLIGRENLIANGIELPDVDGAIVSGTATGDWTLSCVRGSVKSMTFV
                     FNDGRIVSTSNKGNGEEIGWLSDSSGIPCVPGERKTNAPEYLGTNFFLAAASAAAQGF
                     SQSQTTTVVDGSSVVGAVTGDQGKFILGQAIGGGLKESADWFRKRFAENFDAVYVPPG
                     HRVSIHIEKEIEIDYNAQGRKVKYGSKSTNSQLD"
     gene            complement(7089..7973)
                     /locus_tag="PXB27_000923"
     CDS             complement(7089..7973)
                     /locus_tag="PXB27_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03749 family integrating conjugative element
                     protein"
                     /protein_id="HDL5345736.1"
                     /translation="MKRLPILLVSLLIGFSGSSLADVLMKWERKPLPVALQVEKERII
                     FVDKNVKVGYPAELDGKLRIQSTGGAVYLKALETFPSTRIEFRDVTTGEIFLFDLTAA
                     SKVANPEEAIRVVFDGTVEKQSFSSNRDDESSDLPIANSRPQLPVPAALTRYAAQSLY
                     APLRTVEPLDGVRRVAHRLPKKITKLLPAYSIEATPLISWQLDEYVVTAIRLQNKGNT
                     QITLDPRELQGQFYAATFQHNWLGGYGENEDTTTLYLVTEGYANNALIPETRPVKLVS
                     KKVKRTTKVETKPAQSAK"
     gene            complement(7973..8617)
                     /locus_tag="PXB27_000924"
     CDS             complement(7973..8617)
                     /locus_tag="PXB27_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03746 family integrating conjugative element
                     protein"
                     /protein_id="HDL5345737.1"
                     /translation="MSQLKSQLHEKDKHIKTTRIFAFLFFIIILVLAGVIYTFPSRLT
                     IYNPPDLRAGSQRAWWEIPKPTIYAFSYQIFQQMNRWMTNGEEDYEKNITTLKPFLTP
                     HCEAFLRQDYKDRLRNNELRSRTRMVYEITGRGYSEDKVTIFSEDSWAVNLDLSVDEH
                     FQGQPVKRVYIRFPISIVRMDVDSAQNPWGLGFNCYHSIPKLLEGVEPKTEEKK"
     gene            complement(8621..8986)
                     /locus_tag="PXB27_000925"
     CDS             complement(8621..8986)
                     /locus_tag="PXB27_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03750 family conjugal transfer protein"
                     /protein_id="HDL5345738.1"
                     /translation="MSEQDTTIPFLPTRLNREATVYGGMTVSEFGISAGLGFMLGLIV
                     GLFLWILTDFWLLIPAMAMLLCIATVLIGKGIVAAVKRGKPEAYLNRLVEKKMDDLFN
                     GHKFIKREGFWSIRRYRRK"
     gene            complement(9009..9398)
                     /locus_tag="PXB27_000926"
     CDS             complement(9009..9398)
                     /locus_tag="PXB27_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2976 domain-containing protein"
                     /protein_id="HDL5345739.1"
                     /translation="MNRIKSLTKHLHSRYLAVMLFFWGSLQNAFAAGPDASKIPDFQI
                     PGVDSNTKDPMEIIWIVLKGLVTLGAVILAAVAILTVVKALIGIYNEATSENGKKGWG
                     HFILALIIGFIVIFFCLWLVKLAVGLF"
     gene            complement(9413..9646)
                     /locus_tag="PXB27_000927"
     CDS             complement(9413..9646)
                     /locus_tag="PXB27_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03758 family integrating conjugative element
                     protein"
                     /protein_id="HDL5345740.1"
                     /translation="MSYSPANAFSVASGIDPFRLKIMIAIIFLSLLLLAVAWALNSGY
                     KGFSFRDKSPFSLLVLFLKGVALLACVTYFFIY"
     gene            complement(9646..9972)
                     /locus_tag="PXB27_000928"
     CDS             complement(9646..9972)
                     /locus_tag="PXB27_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAQPRD family integrative conjugative element
                     protein"
                     /protein_id="HDL5345741.1"
                     /translation="MSISTSLRKGIVVLVTLSCLPALASTEQEQLAQAIKQLEAAKLS
                     LQRAEKVAKASPKTREYFNYSAIHSDIETVKKGIQQYINPSRALPRDPNVLRTLSDDY
                     SKLRGQ"
     gene            10159..10542
                     /locus_tag="PXB27_000929"
     CDS             10159..10542
                     /locus_tag="PXB27_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345742.1"
                     /translation="MRYQMRYLALFLVVFPYVAHSLPSAQKVGNICHAENAQYNYGGL
                     WELSLRGKLICNYWQFLGDDERKKIELQLDEYGEFPIWNTSEQISEQIIEDDEYPQYW
                     EVEDWEDSDWDTESENEDTEATTEE"
     gene            complement(10574..11269)
                     /locus_tag="PXB27_000930"
     CDS             complement(10574..11269)
                     /locus_tag="PXB27_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03747 family integrating conjugative element
                     membrane protein"
                     /protein_id="HDL5345743.1"
                     /translation="MSEETKARRKSGLINLIFGKINMSLAAIAVSLIFSIILEWIGIA
                     FWWPQEGHLHSQTMMLNEMQWLSDDFSQSLFAKSSTAFATSIIENVYNWLFVYTGVKE
                     WLTSPGNGSMEQWVHHYGRAYIESVIYVVITFIIRLIIIVFTSPLFLLTALSGLTEGL
                     SLRDIRKFGAGRESAYLYHHARRYIMPMMVTAWVLYLSIPFSIHPNIILVPAACLFGL
                     SICITAASFKKFL"
     gene            complement(11266..11619)
                     /locus_tag="PXB27_000931"
     CDS             complement(11266..11619)
                     /locus_tag="PXB27_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
                     /protein_id="HDL5345744.1"
                     /translation="MSSAKMREEKRTNLLDLPNKYRNFNGEFSASCGLDNAEELLIHS
                     QSYFIEWFEQGYSFHQFAEKFADQGLSLWSADEVSMRHSDKSKDIFAFYLAFDNNPSG
                     YILVQCQLDREDSLQ"
     gene            complement(11612..13813)
                     /gene="traD"
                     /locus_tag="PXB27_000932"
     CDS             complement(11612..13813)
                     /gene="traD"
                     /locus_tag="PXB27_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HDL5345745.1"
                     /translation="MSAKHTLEAWLRPPVEFFPAAVHASCAFVSAAAPWSLALHPSIG
                     YSIAAGFGALSFYRAKQGWQIVRYHRNLKRLPNYSMTSKQIPVSNKYLFVGKGFEWKP
                     IHTQRLHDCFSDNGSKYLEPSKLFKMAREYEKRHENWLTHLTSKDSRWNLVRPLPPVE
                     GIPAIHGIEPDEVDVVQSVSSRVGHTVVFGTTRVGKTRLAEVFVTQDIHRGKTADERE
                     VVVFFDPKGDADMLKRMYAEAKRAGREDEFYVFHLGYPEISARYNPVGRFNRISEVAG
                     RISGQLSGAGNSAAFKEFAWRFVNIVARALVELGNRPNYVQISQFVQNIDSLFLDYAE
                     KFIRKTDPKIWENLATIAAGLDEKALPFGMKDRPLIFVINKYIAESAVFDPILQGLAS
                     AVRYDRTYFDKIVASLLPLLEKLTTGKISELLSPDYADILDERPIFDWESIIRKRGIV
                     YIGLDALSDATVAAAVGNSMFADLVSMAGHLYKFGTNDGLPGAFKEKSKPVKINLHCD
                     EFNELMGDEFIPLINKGGGAGIQVTAYTQTLSDIEARIGSKPKAGQTLGNFNTLYMLR
                     VKEPATAELLTKQLHEVTIKRSTVTSSTTDSSNPDDDKAFSSNTGQRISESTVPMLVP
                     ANITNLPKGQAFALMDGSKLWKIRMPLPAVDKDDMMHQSLRELTDYMRKNYHVVENWW
                     QPAFTEYTPSRDIAEAFEAMSEPQYKTQIDHQVDNFGIDEPYDIDEEENDE"
     gene            complement(13813..14319)
                     /locus_tag="PXB27_000933"
     CDS             complement(13813..14319)
                     /locus_tag="PXB27_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HDL5345746.1"
                     /translation="MQNKVKVLLFSLYVSHFSWADLTVIADFGGESAVRFYESLQPEH
                     DENAPNYPNSISASVSEADILPIVSHKLSPGTIKEKPFDLTGMQAIFLIGADNTSAQW
                     LQQNREKLITLNATGLVVNVQTLSELNQLRDIVPELRILPTPGDDLAERIGIAHYPVL
                     ITESGVSQ"
     gene            complement(14322..15092)
                     /locus_tag="PXB27_000934"
     CDS             complement(14322..15092)
                     /locus_tag="PXB27_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HDL5345747.1"
                     /translation="MDITRVLVIVAVFLSNISISQAKPFNGFGSEIDSYIRMAAQRYQ
                     VSEAMLRGFVKMEDGWYGNISPTGATGVGQFTMGTWNWLSETERGKQLGMKLITAKNR
                     GSYADPRRDKYTNTLATALLARWHIEQFQLRNIPITDENLYMAHNIGLDGLHRAMMGK
                     ATKEDILNMRRNGMKSWMTVRDFLNYQKGRFLAHKQEANFMTVSKQGHIHTGVAAFVY
                     PTSQMGKSVKWVEPDNKMIWVEPKNAMVWVEPQNTLQR"
     gene            complement(15071..15844)
                     /locus_tag="PXB27_000935"
     CDS             complement(15071..15844)
                     /locus_tag="PXB27_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03759 family integrating conjugative element
                     protein"
                     /protein_id="HDL5345748.1"
                     /translation="MREISMKFNLCRKAIPLAVLPLLLSFALPTLAQNMQTETVSSSQ
                     STLQKQSQHQQSQLSDSLKQAKEWGLTVEEWSRYTELMKGERGMWSPNLDPLTALGIE
                     ARTAEEREKYARMLAKKYYERVSKELDFDKVYRQEFEKLYPNELPFEVEPHISQSVGR
                     VVYFTRFDNCDSCKSDLGKILSYANNQTPLDIYIVGQNISDNDIRKWAADNNIDPIKV
                     SKRLITLNHDKGYWLRYSLGKMPAAYQIKQDGEWTSLVY"
     gene            complement(15844..16473)
                     /locus_tag="PXB27_000936"
     CDS             complement(15844..16473)
                     /locus_tag="PXB27_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345749.1"
                     /translation="MKTKISLMTVFMLGLSGCVHQQDTAQNSDEITPMPDSLLTPQMQ
                     QLPPQDRIVSKDIYQEQTTSNTEVVRDGRYTLISIAPEEGQKYLLEQVVSVKVSNPKN
                     RFTANVQQGLRSTLTNTGLDLCYPPIGEQGNAVQTLFTRPLPKVHYQFGPMKLYEALQ
                     MIAGPAYELTLNDISRTICFKARESSFQNPHKPAVAVTTTTIKTEVIEE"
     gene            complement(16632..16892)
                     /locus_tag="PXB27_000937"
     CDS             complement(16632..16892)
                     /locus_tag="PXB27_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345750.1"
                     /translation="MTFELGRVCTTRAVAECFNQNALVELLNRYCYGDWGNLCEEDKL
                     ANQEAMKYGGRIVARYDVDDESLYIITEADRSCTTILFSYEY"
     gene            complement(17005..17367)
                     /locus_tag="PXB27_000938"
     CDS             complement(17005..17367)
                     /locus_tag="PXB27_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345751.1"
                     /translation="MEDSFFDFDDISCYLGQWEAILEEYSDIVSIEDFWLVAKEFETV
                     PHFGNLYQELVISRLIQRFCTELDIEQDSDLVEFDYYINAIDTHFYINRQRICDIDDW
                     NEMLDKIRKEMTPAKLAA"
     gene            complement(17447..17854)
                     /locus_tag="PXB27_000939"
     CDS             complement(17447..17854)
                     /locus_tag="PXB27_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization protein"
                     /protein_id="HDL5345752.1"
                     /translation="MNRQGIYINGKQVFDSKGKEINSVQIINSTVQTVVGINKGYISM
                     AAGNNVLIVDGQTIALEPSPTINIEVFGNVTNLNAGSENVRVTGDAKSVSTVSGDVTV
                     DGNVTSNVKTVSADIHCKSILGKINTVSGDIYR"
     gene            complement(17880..18560)
                     /locus_tag="PXB27_000940"
     CDS             complement(17880..18560)
                     /locus_tag="PXB27_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345753.1"
                     /translation="MNSLQQFVKTLSPTVLNGRHFDGIVGDVVFVQNGYGKWLDQGFT
                     IIGFDKSIEEKDWIYLDWDCYWFGKNPAQVIPEKSWAQRSWLARAYSISLERFNSLGL
                     EREYNIVSDWDNPIYGTDIQLIEHSKEFFRWTEADDRGIFLVHNEMAFHFFKKEIKAS
                     SEPEYYQPIETFWKDATVVLNIEDLDMLERVGLEQTNTPALAKLTTFVQAARNEISNG
                     NIVFYRAF"
     gene            complement(18666..19097)
                     /locus_tag="PXB27_000941"
     CDS             complement(18666..19097)
                     /locus_tag="PXB27_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3577 domain-containing protein"
                     /protein_id="HDL5345754.1"
                     /translation="MTTQTSTQKNYFNLHTTGIGYLNDIRLVEHKKGNPFYACRVAAL
                     VGESDSPEYRYFDMNVVGKETETLIKKCQKAVSEEKKVLVSFVMADLWFDTFTYSSDS
                     KYHKKGDTGVSLKGRLIRIKMIKIDGEIVYQEKPKADEQSE"
BASE COUNT         5745 a         4059 c         3665 g         5898 t
ORIGIN      
        1 gatttaaaat cacttttttc tgatgatgaa gaacaagaag atgatgaata tcaatatggg
       61 tatgatggac caggtatata tctaggtaat taccgtgttg atgagtaaaa gtaaatagag
      121 tggtatatac cactctatta ttcagttgtt agattatttc cctatcttgg aacccgctgt
      181 tttagagtct ctagaaccag tagttaaatg acttgcaaac tctcccattt ttactcctac
      241 ccaagacata ctgcctagcc aaatcgctgg taagaccaag aacatagaac ccatcacaaa
      301 attcacaata atatcgtctt gtgtattttc aaggaaataa gggtttattc tgctatgtgt
      361 gcctgaatca tagaggaccg acagcaacca agagtcaagc catctagcta gttcccacca
      421 aaatgtgagt gtaactaatg cgaaattcac aaaagttagc gtaaccaatg tttttaacga
      481 ataaccactc ataaccataa ttaaaggcgt gagaataatc aatgccatta atgcaaatga
      541 atgcaccatc ggtaatgctt gccgcatcga atcaaaagca gggaatgctc cgatactgcc
      601 taatgacaga ccaaacattg aagctgtttt agtcacttca tttgtaaatg atctgtctaa
      661 atttccccca tacccgggat aaactttccc tctcgaaata tccatattct cagctcgtaa
      721 tacggtacga agtatggatt catcggaatt ttctttactg ctgaaaaaat caatcacttt
      781 tgcagagaag ttctgtttta catcatctaa taaacgatct tttaaaccat tattcgccgt
      841 atcccaccac tctgcacaat ttggaaaacc accctctcca ttattcggca atccattatc
      901 ccgagaagca ttatagggaa atagactatg aggttgttgg gctcgataac ggggataaag
      961 ctcgctattt gcaatcaaaa tacggccacc taaccagcta gtctcacgaa cctgtgctgg
     1021 gctcaatgaa agttggctat tttgaatttt ctgacgtgcc ggtacatagc attgttgtac
     1081 aaataagcga acctctgtca ataatgctgg attattgatt ttctcgtgtt gaacttcaaa
     1141 acgaacttga cgtaaatccg gtttgcaggg aattgaagct gttaaggcgt gtgttacccc
     1201 tttgttgatt tgatgaaata atccccacca gatagggact ctcgcttgtt gatcacccaa
     1261 ttcagagttc atttggttat aaccactatc ctctggtgca gttggaacac gataaccaca
     1321 ggctttagaa cgttctacat tcaactcaat attattcagg ttgatcggta tcattggagc
     1381 taagaccaat acaacgacaa acatcgccgg gtaaaatttg aggctgagcc aagtaataag
     1441 taaatcccct ttattacctt cgtcctcacc ttgttttttg acttccatcc acgtactgat
     1501 taagacaaaa atgagaggca gaacaaacaa ccctgtttga accaatacag cgaaaatatt
     1561 gttgttgatg atccaagcca gtaaggtaag aaagtattct aagtaactat ctacggtaaa
     1621 ggtcatctca tcctccttaa agtagataca gtaaattaag tccctcaata attgggatga
     1681 cagataaaat cattaacctg gcttttaatg gcatttgccc ttttcgatta agttgtacca
     1741 tcaatagcca aatagctaca tataaaacaa atcgccatcc caaccaggca tagcgggttt
     1801 catagaacca cgcttgaaaa tttgtggaag gaaaataggt cattgccaac cagccaacaa
     1861 cgagtagtaa taaaataata ccgatgatca tcaaggttat ttttaataca gtaataaccc
     1921 ttgtcatttt aggctttgtt gtatcagact tcattattat ctattacctc gttatacaca
     1981 gagttatccg caattgatgt ggataagggt aaaagattac ttttcaaacc gagatactga
     2041 gtaaggaatg ccgaaaacca ataagcatcc acatgatcag ctatttcaat aattaaacga
     2101 gctgaccatt cactgttatt ggaagttggt agcaaagaac gccccgtcac attaagtaat
     2161 aagtgaacag aaccctgctc accagaaagt tcgatagccc agtgattttg cacgacacga
     2221 tgagggaata aggtaatctg tttcccaacg ccgatgagtt ggcgaatttg ttcacaaaat
     2281 ccaagcaatt gagtttctac cgctggagat acaactccta atgaggattt ttgtgggcct
     2341 gcattaaata aacggtaatg ttgtttattc attagtgtat tcctcttgct ttatcaatcc
     2401 gagctgcaac ctctaaagct gcatcgagtt cggtatagcc gtgctgcatc atcagttgct
     2461 tacgttctgc tttttcatgt ttttcagtca ttcctaaagc taaaaataag ctaggcggta
     2521 caactcggaa tagagcttcc atattcgttg ctaataccac gccctcggta tatttacgat
     2581 ctgatttttt ggcagataac aacatcgttt tctgggcctc tgacagtttc cggaaacgat
     2641 tgatttcatc aatttcactg gcagacacat tcaacaattc ccaccattcg accattgaga
     2701 gtaatttcgc cgcttcattc gggaagtcat ccatattttg agtagcaagc catagccaaa
     2761 tccctagttt acgccacatt tttgagcctt taactaggaa tttagctaat aatgggttgg
     2821 ccgtgataat gtgtgattca tctgttacat tcagaatcat ccgatcactg tgttggtatt
     2881 tttcaccaag actgttgata tggttaatga gtgaaatata ggaaatggcc aactctgctt
     2941 catagccttc ccgggcaaac atagctaaat caactaaggt tagatcagcc tccggccaaa
     3001 ttgccccctc tcgattaaaa aattgcccat ttacaccggt acaaaacatt tctaatgatt
     3061 ctgccatttc tgctaaacgg gtacgccgtt cttcacgagt atgctcatca tgacttaatg
     3121 cttccaaagc atttttaata tcttcagtca gcgtttgtct gccttcagat ttggtttggc
     3181 gagctgcatt taaaatggca cgacgaatag cagcacgatc agcacggctc attttttcat
     3241 cttctttctt ttcacctccg gtgatcatca aacgtgccgt cagctccatt tcggccaata
     3301 aatctcgttg catatcacct tcatcatcag tgtcatcgtt atcctcaatg tcttccggca
     3361 catcttccgg gtttaattcc atatccaata gacgatgtgc ttctgaaaag agtggtaaga
     3421 caatgccgct gtttggagct aacgtaatgc gatttacagt cagtccgaaa cgttctgcat
     3481 agtccgctaa taaaccaaat gaatttcctg cctcaacaac aaaagtccgt gggcgataaa
     3541 cggccatagc ctgacataat tgcccacata acgtagctga ttttcccgaa ccggttggac
     3601 ctaataatag cttgtgagca tttttggttc tgtctttacg gttaaatggg tcgaaattaa
     3661 atggtgctcc tcctcgatta aagtaagtga tccccggatt cccagtcccg gtatctcggc
     3721 caaagatagg taataagttt gccaaatgtt gtacaaaata gtacttcgtg tagtaatgtg
     3781 atttggcatc catctgcgga ttaaaattca ttggtagcca acgcaaatag ctgttaagtg
     3841 gggcaatttc tgcattttcc ttcacgggtt ctaggttgtt actgagcaga atcgttctca
     3901 actggcgaga acgggtacgt agctcctttt catccttacc tcgaacgtaa aatgcaatag
     3961 ctgcttgata gagtttatgc ccctctttaa gataagactg tacagtttga cagtcttctt
     4021 ttaggtattt cgactcaaaa ttttcaccta ccgctcgact actaattttt tctaaatgtg
     4081 cctccaactt atcctgtgga tggatgacga tggtaattgc cataatggtt gattccggca
     4141 acaagtcaaa taatgcattg atattacgcc cacgagtaat ctcgccagtc atcaacccca
     4201 cttccggttg tttcctcaaa ttattgacta caacaacttc gtgcggttgg tcatcaaaat
     4261 accaatagtt gccttcattt ttcggttgag aataaaataa atcttcactg aaatcgttaa
     4321 acgctaattc tggaagcccg tcatttgagg tttgtttacc aaaaagatgg tagcaatcat
     4381 tgttgcttaa cccctcaaaa ttaatagaag gattgggatt aaaccaacgt aacagccatt
     4441 gacgaaattg ttcgccatcc agtcgctgca attgtaatcc agcagttgtt agagctccat
     4501 ttatgcgaga acaagcattg ttcagctgct cgacaatcag attttcatta gtgtcattgg
     4561 atttatcaac ataccgatag atcaccattt tggtactacg tatacgacca gaccaagctg
     4621 tatttgtcac tcgctcatca ataaaaagcc cctctggttt accgatatcc ttgaaatgct
     4681 tggcttgtac ccgtaaccat tcttcgctaa atgcactatt tttagcgata ggcgagatat
     4741 aatttcgtag ctgttcaata taaggctgta aatcatcgct gtcttggcaa taaaattgaa
     4801 caacgtacgg atttacatcc aattcatcaa agctgtcttg caaggcatct ttgacaagat
     4861 tacgtatccg acttaaaaat tcaatactac ggccagcagt accaatcggc ttaatatcaa
     4921 atactgcccc aacactacgg gcatcatcaa ggagtatcac accgtgctca ttcaaatatt
     4981 ctccccaagg tagatgatcg ataaaggagg gctcttgtga gtataaccga cggtgatgct
     5041 gtgactgggt gagttttcca ctgcgtttta attgttgaac ctcgttattt acctcactca
     5101 ttttattttg aatgatgtca gactcaccgg tattttcatt tggttcactt gatataaccc
     5161 ctgctttagt cggtttgaaa gaggaaaata cctcacttaa aaaagacatg attaaagccc
     5221 tcttgttcta tcacccggct gagcatattg aactcgtcca tagaaaggta gtactgtaga
     5281 atatcctgga atactgagag gctctccgga acgagtttga tgtgggtaca catacataat
     5341 cagatccgga ttaggtaaac gagggaacaa gttattaatt tcattttctg ccgtacgggt
     5401 ataggctcgt tgctcactat taggaatata atcgacaggg tcaagtgttc gcccttgttg
     5461 tgaacggtat aagtgtagat cgttattact cgctccttta tcccaaatat cccgcatcgt
     5521 actgccgtta gtaggtaata attcttctgt tgatgttgtg caacctgtta tcaataaggc
     5581 acttatcatt atgcaaaata aatgtttcat cgtttgttcc ttctaatcca gttgtgaatt
     5641 cgttgatttt gagccatatt tcactttacg accttgggcg ttgtaatcga tttcaatctc
     5701 tttctcgata tgaatagaga cacgatgacc cggtggcaca tatacagcat caaagttttc
     5761 agcaaaacgt tttctaaacc agtcagcaga ttcttttaag ccgcctccga tagcttgtcc
     5821 taagatgaac ttaccctgat cgccggtaac agctccaaca acactacttc catcaacaac
     5881 agtggttgtt tgtgactgtg agaatccttg ggcagcagca cttgcagcag ccaaaaagaa
     5941 attcgttccc aaatattctg gggcattggt tttccgctct cctggaacac aagggatacc
     6001 cgaactatct gataaccagc caatctcttc gccattccct ttattagagg tactcacaat
     6061 acgtccatca ttgaacacaa aagtcatact ttttacactg cctctaacac aggatagtgt
     6121 ccagtcgcca gtagcagtac ctgatacaat tgctccgtct acatcaggta attcaatacc
     6181 attagcaatg agattttctc gaccaataag tactttgaac ggataagcat ccgatactcg
     6241 attatcaatc gggatacgtc cgatgagtcc cgtcattgca actgaatcaa ggaatgttga
     6301 attacttggt atggtatata ctgatgtcat ctggttttta ccacgtgcgt ttccagccgt
     6361 tccttgtgag cttgtcacag tatctgtttc cgggaaaaac ggcagacctt tcttttcgtt
     6421 atttcctgca gattgtcctc cattaaaatc tttcggaaag ccaaatttag ttgctactgt
     6481 attgcctgac acaggttttc cacttgcatc caaactaatc tgatcgcttg gttctaccca
     6541 atacattcca ctggtatcca cgttggacat agttgcacca ccgataggta atttatctcc
     6601 tgctgttcca aatcctttat tcggtaccgt attaggctga gcctctacct gaggggtaga
     6661 ctcttgtgtt gttggtttgt tattaacgag agtcgccata tcaaataatt gcattttcaa
     6721 atctgcaatc tgctcttgca gttttcgggt ttcattattt accgcatcat tgatacgacc
     6781 attcactaaa tcttctttct gtttgagttg gcgattttca tcaattaact catccatacg
     6841 acgctcattg gatttaacgg ttgtcacaag ggtacgcagc gtatcaatcg gcgtatcacc
     6901 ttgaataccc attgttctga cttgttcagg tgttaaatct ttaatggaaa tatctaaaat
     6961 ttcctgttgt gtcggttgtg gaactgactc actacgtcca aataaggcat agacaattcc
     7021 accgactaag ataaatacaa tgcccagtat gacaaataag gctttattcg ctttcattgc
     7081 ctttcctcct acttcgccga ttgagccggt ttagtttcaa cttttgttgt tcttttcact
     7141 tttttactga ctagtttgac tggacgggtt tccggaatta atgcgttatt agcatagcct
     7201 tcggtcacta aatacagggt tgtggtatct tcattttctc cgtacccccc aagccaatta
     7261 tgctggaaag tcgctgcata aaattgtcct tgaagctctc gagggtcaag cgtaatttga
     7321 gtgttaccct tattttgcaa acgaatagcg gtaactacat attcgtctaa ctgccagcta
     7381 attaaaggtg ttgcttctat tgaataagcc ggtagcagct tggtaatttt tttaggtaga
     7441 cgatgtgcca cacgacgcac accatctaaa ggttcaactg tacgtagtgg agcataaagt
     7501 gattgtgctg cataacgggt caatgcggca ggtactggaa gctgaggacg gctattcgca
     7561 ataggcagat cagagctttc atcatctcta ttgcttgaaa aagattgttt ttctaccgta
     7621 ccgtcaaaaa ccactcgaat tgcctcttca ggattagcga ctttcgaagc tgctgttaaa
     7681 tcaaatagaa aaatttcacc ggttgtaaca tcacggaatt ctatgcgagt ggagggaaat
     7741 gtttctaatg cttttaaata gacggctcca ccggtagatt gaattcgtag cttaccgtcc
     7801 agttctgccg gataaccaac cttgacattt ttatcgacaa aaataattcg ttctttttcc
     7861 acttgtaacg caacaggaag tggttttctt tcccatttca tcaatacatc agccaagcta
     7921 ctcccagaga agcctataag caaagagacg agcaaaattg gtagacgttt cattattttt
     7981 tctcctctgt ttttggctca acaccttcta agagttttgg aatggagtga tagcaattaa
     8041 accctaatcc ccaagggttt tgagcagaat cgacatccat tctcacaata ctgataggaa
     8101 aacgaatata aactcgtttt accggctgtc cttggaaatg ttcatccaca cttaaatcaa
     8161 gattgacagc ccaactatct tcactgaata tggtaacttt atcttcacta tatcctctac
     8221 cggtaatttc atacaccata cgggttcggc tgcgtagttc attgttacgc agacgatctt
     8281 tataatcttg tcgtaaaaat gcttcacagt gtggtgttaa aaatggtttg agggtggtga
     8341 tatttttctc ataatcctct tcgccatttg tcatccaccg attcatttgc tggaaaattt
     8401 gataggagaa tgcataaatt gttggtttag gaatttccca ccaagctcgt tggctcccag
     8461 cacgcagatc tggtgggttg tatatcgtaa ggcgacttgg gaaagtatag attacgcctg
     8521 ccagtaccaa aataataatg aaaaacagga aggcaaaaat tcgagtcgtt ttgatatgtt
     8581 tatctttttc gtgtaattgg ctttttaact ggctcatttg ttatttcctt ctatatcggc
     8641 gaatagacca aaacccttct cgttttataa acttatgacc gttaaagagg tcatccattt
     8701 tcttttcaac taatctattt aaataagcct ctggttttcc tcgtttaacc gcagcgacaa
     8761 taccttttcc gattaagaca gttgcgatgc agagcaacat tgccattgcc ggaataagaa
     8821 gccaaaaatc agttaatatc cacaagaaga gaccaacaat cagacctaac ataaatccta
     8881 atccggcaga tatgccgaac tcactaactg tcatcccacc gtaaacggta gcttcacgat
     8941 taaggcgggt gggaagaaaa ggaatggttg tatcttgttc tgacatattg gtctcctaga
     9001 cattttaatt agaagagtcc gactgcaagt ttgactaacc acaagcagaa gaaaatcaca
     9061 ataaagccga taattaaggc gagaataaag tgtccccaac cttttttacc attttcagat
     9121 gtagcttcgt tatagatgcc aatcaatgct ttaacaactg tcaaaatggc tacggctgct
     9181 aaaataacag ctccgagagt gacaagacct ttcagaacaa tccaaataat ttccattgga
     9241 tctttggtat tactatccac gcctggaatt tggaagtctg ggattttgga agcatcagga
     9301 ccggctgcaa atgcattttg gagggaaccc cagaagaaga gcattacagc caaataacga
     9361 ctatgcagat gtttcgtaag agatttaatg cggttcataa tagacctcga atctaataga
     9421 taaagaaata agtaacacaa gctaacagtg ccactccttt gagaaataaa actaaaagtg
     9481 aaaaaggact tttatctcta aatgaaaatc ccttataacc actattcaat gcccaagcga
     9541 cggctaagag cagtaatgaa agaaaaataa tggcgatcat gattttcaat ctaaaagggt
     9601 cgataccgct ggcaacagaa aacgcattcg caggggagta agacatcact gcccccgtaa
     9661 tttgctataa tcatcactta atgtacgtag tacattcgga tcacgaggta atgctcggct
     9721 tggattgata tattgttgaa ttcctttttt gacggtttca atatcactgt gaatagcaga
     9781 atagttaaag tattctcgtg ttttaggcga ggctttagct actttttctg ctcgttgaag
     9841 tgataatttt gccgcttcca gttgcttaat tgcctgggct aattgctctt gttctgttga
     9901 agcaagtgct ggcaagcaag ataaggtaac aaggacaaca atacctttcc ttaaagatgt
     9961 tgaaatagac atagtgcacc tctattttta gttttacttc aggttaagag tgccaaaagc
    10021 gatcctaaaa tttaatggaa aactctgtta ccgttcagaa gagaaattgc acaaaatagt
    10081 aaatggtgag aaagatatta ttctatgttt aagctagtca attaagtagt atcaacaccg
    10141 aaatcttcac gctttacgat gagataccag atgagatatc tagcactgtt ccttgtagtt
    10201 ttcccatatg ttgcccattc tcttccttca gcacagaaag tggggaatat ttgccatgca
    10261 gaaaatgctc aatataatta cggaggtctt tgggaattaa gtttaagagg aaagttaatc
    10321 tgtaattatt ggcaatttct aggcgatgat gagcgaaaaa agatagagtt acaattggat
    10381 gagtacgggg aatttcctat ttggaatacc tctgaacaaa tttctgaaca aatcattgaa
    10441 gatgatgaat atccacaata ttgggaagtc gaagactggg aggattctga ttgggatact
    10501 gagtcagaaa atgaagatac agaggcaaca acagaagaat aagcccccgc atattgctaa
    10561 gggcttatga aatttacaag aatttcttga aactcgccgc agttatacag atggataaac
    10621 caaacaaaca ggctgctggg actaaaatga tgttcggatg aattgaaaac gggatagata
    10681 aataaagcac ccaagctgtg accatcattg gcataatata acgtctagcg tgatggtata
    10741 ggtaggctga ttcccgcccg gcaccaaatt ttcgtatatc tcgtaatgac agtccttcgg
    10801 ttaaaccgga aagtgcagtc aataaaaaga gtgggctggt aaaaacaata ataatgaggc
    10861 gaataataaa ggtgatcacc acataaataa cagattcaat ataggctctg ccgtagtgat
    10921 gtacccactg ttccatacta ccattacccg gtgaagttaa ccactcctta acacccgtat
    10981 aaacgaataa ccagttatac acgttttcaa taattgatgt tgcaaaagca gtagaagact
    11041 tggcaaacag gctttgagaa aagtcatccg atagccattg catctcattg agcatcatcg
    11101 tttggctatg caaatgccct tcttgcggcc accagaaagc aatgcctatc cactctaaaa
    11161 taatgctgaa tatgagagat actgcaattg cagcaagcga catatttatt tttccaaaaa
    11221 tcaggttgat taaacctgat tttcggcgag ctttagtttc ttcactcatt gcaaactatc
    11281 ctctctatcg agctggcact ggacaaggat atagccggaa ggattattat caaaagcaag
    11341 ataaaaagcg aaaatatctt tggatttatc cgagtgacgc atagatactt catcggcaga
    11401 ccataacgaa agcccttgat ctgcgaattt ttcggcaaac tgatggaagg aataaccttg
    11461 ttcaaaccat tcaataaaat aggattgact atgaatcagg agttcttctg cattatctaa
    11521 gccacaggaa gctgaaaact ccccattaaa attacggtat ttatttggta aatctaatag
    11581 attggttctt ttttcctctc tcattttggc actactcatc attttcctct tcatcaatat
    11641 cgtaaggttc atctatgccg aaattatcta cttgatggtc tatctgtgtt ttatattgag
    11701 gctccgacat cgcttcaaat gcttctgcaa tgtctcggga cggcgtatat tctgtaaatg
    11761 ccggttgcca ccaattttca acaacgtgat agtttttacg catataatct gtcagctctc
    11821 gtaaagattg atgcatcata tcatctttat cgacagcagg taacggcatt cgaattttcc
    11881 ataatttact accgtccatt aaagcgaatg cttgtccttt tggtaaattg gtaatgttgg
    11941 ccggaaccaa cataggaacg gtactttcgc tgatacgctg tccggtattg gagctaaacg
    12001 ccttatcatc gtcaggatta ctggagtcag ttgtactgga cgtgaccgtg ctacgtttaa
    12061 tcgtcacttc gtgtaactgt tttgtgagta attctgccgt agccggttct tttaccctca
    12121 gcatatagag tgtattgaag ttacctaagg tttgtcccgc tttcggttta ctgccgatac
    12181 gagcttcaat gtcggagagt gtttgtgtat aagcagttac ttgtattcca gcaccaccgc
    12241 ctttgttgat gagagggata aattcatcac ccatcaattc attaaattca tcacaatgaa
    12301 gattaatttt cactggtttg gatttttctt tgaatgctcc cggtaatccg tcatttgtcc
    12361 caaatttata taaatgacct gccattgaga ctaagtcagc aaacatactg tttcctacag
    12421 cggccgcaac tgtcgcatca gagagagcat ctaaaccaat ataaacgatt cctcgtttac
    12481 gaataatgga ttcccagtca aaaatcggac gttcatccaa aatatcagca taatccgggg
    12541 ataacagctc agaaattttc cctgtcgtta atttttccaa taacggcaac aatgaggcaa
    12601 caattttatc gaaataggta cggtcatagc gtacagcact ggcaagtcct tgtagaattg
    12661 ggtcaaagac tgcactttca gcaatgtact tgttgatcac aaaaatcagc ggacggtctt
    12721 tcattccgaa aggaagtgct ttttcatcca gtccagcggc gatggttgct aaattttccc
    12781 aaatttttgg atcggtttta cgaatgaatt tttccgcata atcaaggaat aatgaatcaa
    12841 tattttgtac aaactgggaa atttgaacat agtttgggcg atttcccaat tcaactaatg
    12901 cacgtgcgac gatattgaca aatcgccaag cgaactcttt aaatgccgcc gagttacccg
    12961 ctccacttag ctgtcctgaa atacgcccag cgacttccga aatacgatta aagcgtccta
    13021 ctgggttata acgggcagaa atttcgggat aacctagatg gaaaacataa aactcatctt
    13081 ctcgccccgc tcgttttgcc tccgcataca ttcgtttgag catatcggca tcgcctttcg
    13141 gatcaaagaa gactaccact tcacgctcat cagcagtttt acctcggtgg atatcctggg
    13201 taacaaagac ctcagctaaa cgagttttac cgacacgtgt tgtaccgaat actaccgtat
    13261 gcccaacccg agagctaacg gattgaacga catcaacctc atccggctca attccatgga
    13321 tagcaggaat accctcaaca ggtggcagag gtctaaccag attccaccgg ctatcctttg
    13381 aggtaagatg tgttagccaa ttttcgtgtc gtttttcata ctcacgagcc attttaaata
    13441 gcttagaagg ctccagatat tttgaaccgt tatccgaaaa acaatcgtgt aaccgttgag
    13501 tgtgaatagg tttccattca aatcccttac cgacaaacag atacttatta ctgaccggaa
    13561 tttgcttgct tgtcattgaa taattaggca aacgtttcag gttgcgatgg taacgcacaa
    13621 tttgccaacc ttgttttgcc cgataaaagc tcaatgctcc aaaaccagct gcaatactgt
    13681 aaccgatact tggatggagt gccaaagacc aaggagctgc agcactgaca aaggcacaag
    13741 aagcgtgaac cgctgccgga aaaaattcaa caggaggtcg taaccacgcc tctaaggtat
    13801 gtttagcaga cattattgtg aaactccgct ttcagtgatt aagacagggt aatgtgcaat
    13861 accaattcgc tctgcaaggt catctccggg agttggtaat attctcagtt ccggtacaat
    13921 gtctcgtaat tgattaagtt cggagagtgt ttgtacattc actacaagcc ctgttgcatt
    13981 caacgtgatc aacttctctc gattttgttg taaccactga gctgaggtat tatctgctcc
    14041 aattaagaaa atggcttgca ttccggttaa atcaaatggt ttttctttaa tagtcccggg
    14101 agatagttta tgggaaacaa tgggtaaaat atcagcctca ctcactgagg ctgaaatgga
    14161 attcggatag tttggtgcat tttcatcgtg ttccggttgc aaagattcat aaaaacgtac
    14221 cgcactttca ccaccgaaat cagcaatcac ggttaaatca gcccaagaga aatgggacac
    14281 atataaacta aacaataata cttttacctt gttttgcatt tttacctctg aagagtattt
    14341 tggggttcta cccaaaccat tgcattttta ggttcaaccc aaatcatttt attatccggc
    14401 tcaacccatt tgaccgattt tcccatttgg cttgtgggat aaacgaaagc tgcaactccg
    14461 gtatgaatat gcccttgctt ggagacagtc ataaaattgg cttcttgctt atgtgctaaa
    14521 aaacgtcctt tttggtaatt gagaaagtct cttaccgtca tccaactttt cattccatta
    14581 cgacgcatat ttaaaatgtc ttctttagta gctttcccca tcattgctcg atgcagccca
    14641 tctaagccga tattatgagc catatataaa ttctcatcag taatagggat attacggagt
    14701 tgaaattgct caatatgcca acgtgccaat aatgccgttg ctaaggtatt cgtatattta
    14761 tctcgacggg gatcggcgta agaaccccga tttttggctg taatcaactt cataccaagt
    14821 tgctttcccc tctctgtttc agatagccag ttccaggttc ccatagtaaa ctgcccaaca
    14881 ccggttgctc ctgttggtga aatgttgcca taccagccat cttccatttt gacaaaacct
    14941 cgtagcattg cttcactcac ctgataacgt tgagcagcca tacgaatata gctgtcaatt
    15001 tcagaaccaa atccattgaa gggctttgcc tgagaaatgc taatgttgga aagaaagact
    15061 gcaacaatga ctaatacacg agtgatgtcc attcaccatc ctgtttgatt tggtaagcag
    15121 cgggcatttt tcctaacgaa tatctcagcc aatagccttt atcgtggtta agggtaatca
    15181 atcttttact gacttttatt gggtcaatat tattgtcggc agcccattta cgaatatcat
    15241 tatcggagat gttttgccca acaatataaa tatcgagagg tgtttgatta tttgcgtaag
    15301 agaggatttt cccaagatca cttttacaag aatcgcagtt atcaaaacgg gtaaaataaa
    15361 ccacacgacc taccgattga gaaatatgtg gttcaacctc aaaaggtagc tcattcggat
    15421 aaagtttctc aaactcctga cgataaactt tgtcaaaatc aagttctttt gacactcgtt
    15481 cataatattt tttcgccaac atacgagcat atttttccct ttcctcagca gttcgtgctt
    15541 cgatacctaa agcagttaac ggatctagat ttggcgacca catacctcgc tcacctttca
    15601 ttaattcggt ataacgactc cattcttcaa cggtcaatcc ccattcttta gcctgtttta
    15661 ggctatcact taattgtgat tgttgatgtt ggctttgttt ctgcaaagtc gattgtgagc
    15721 tacttacggt ctctgtttgc atattttgag ccagagtagg taatgcaaag gacaataaaa
    15781 gaggtagtac tgccaacggt atagctttac ggcataaatt aaacttcatt gatatttccc
    15841 tcattattcc tcaatgacct cggttttaat tgttgtcgtt gtaacggcaa cagcaggttt
    15901 atgagggttt tggaatgaac tttcccgagc tttaaagcaa atcgtacggg atatatcatt
    15961 aagagttagt tcataagcgg gacctgcaat catttgtaga gcttcatata atttcatcgg
    16021 accgaattgg taatgaactt ttggaagagg tcgggtaaaa agtgtttgaa ccgcattacc
    16081 ttgttctccg ataggtggat aacacaaatc taaacccgta ttagttaatg tagaacgcaa
    16141 accttgctgg acattcgcag taaaacgatt tttaggattg gagactttga ctgatacgac
    16201 ttgttcaagc agatattttt gaccttcctc cggagcaata ctgattaagg tgtagcgtcc
    16261 gtcacgtacc acttcggtat tcgaggtagt ctgttcttgg taaatatctt tggacacgat
    16321 acggtcttgt ggaggtaatt gttgcatttg aggtgttagc aatgaatcag gcataggtgt
    16381 gatttcatca ctattttgag ctgtatcctg ttgatgaaca caaccactta agccaagcat
    16441 aaaaacagtc attaaggata tttttgtttt catttaatct ctccaagtat gaaaggaatc
    16501 tagaccaact atggcgaagt cattgctgat tttgaatgat ttattctgtt gttgattgag
    16561 agaataaaaa aacgccatca ttagacggcg tttgaaaaca caataatgtg ctgtgttaat
    16621 cggatttctc cttagtattc ataagaaaat aagattgttg tacagctacg atcagcttct
    16681 gtgatgatat aaagactttc atcatctaca tcgtagcgag ctacaattct ccctccgtat
    16741 ttcattgctt cctgattcgc taatttatct tcctcacaca aattgcccca atcaccataa
    16801 cagtaacgat taagcaattc aaccaaggca ttttgattga aacattcagc aacagcacga
    16861 gttgtacata ctcgaccaag ttcaaacgtc atacatttct cctaaataaa atacggggaa
    16921 atgtcaccca ttaggggagt atttccccaa gtgggtggac taagactgcg gcaagagtcg
    16981 tttttagtaa aaatttgcaa aaaattaggc agctaattta gccggcgtca tttctttacg
    17041 aatcttgtct aacatttcat tccaatcatc tatatcacag attctttggc gattaatgta
    17101 aaaatgcgtg tctattgcat tgatatagta gtcaaattca actaaatctg aatcttgctc
    17161 aatatctaat tcagtacaga atcgttgaat taagcgagag atgacaagct cctgatacag
    17221 attaccgaaa tgtggtacag tttcaaattc tttggcaaca agccaaaagt cttcaatact
    17281 gacaatatcg ctatattctt ctaagatggc ttcccattgt cctaaatagc aagagatatc
    17341 atcaaagtca aaaaaggaat cttccattat gtttctccaa gttttaaata aaaagtgggg
    17401 aaacatacct accgagaatg tttcccgata gggtgtatag tcaatattag cgataaatat
    17461 caccgctcac tgtgttaatt ttacctaaaa tagatttaca atgaatgtca gcactcacag
    17521 ttttcacatt cgaggttaca ttaccatcca ctgtgacatc accactgaca gttgaaacag
    17581 atttggcatc gcctgttaca cgaacatttt ctgaacctgc attgaggttg gttacattgc
    17641 caaatacttc aatgttgata gtcggagagg gttctaatgc aattgtttgt ccatcaacaa
    17701 ttaatacatt attgccggca gccattgaga tataaccttt atttatccca acaactgttt
    17761 gcactgtact attaatgatt tgaacgctgt tgatctcttt gcctttagag tcaaagactt
    17821 gtttaccgtt gatataaata ccttgtcgat tcatataata atcctctttg tattagatgt
    17881 taaaatgctc gatagaatac gatattacca ttggaaattt cgttgcgagc agcctgtaca
    17941 aacgtagtta attttgctaa agcaggtgta ttagtttgct ctaatcctac tcgctctaac
    18001 atatctagat cttctatgtt caataccacc gtagcatctt tccagaatgt ctcaataggt
    18061 tgatagtatt ccggttccga tgaggcttta atttcttttt tgaagaaatg aaaagccatc
    18121 tcgttgtgaa ctaagaaaat ccctctatca tcggcttcag tccaacggaa aaactccttg
    18181 gaatgttcaa ttaactgaat atctgtacca taaatggggt tatcccaatc agacacaatg
    18241 ttatattcac gctctaagcc taacgaatta aaacgttcaa gagagattga atacgctcta
    18301 gcaagccaag aacgttgagc ccaagatttt tcagggatta cctgtgccgg attttttccg
    18361 aaccaatagc aatcccaatc taggtaaatc caatcctttt cctctatgct tttgtcaaaa
    18421 ccaataatag taaagccttg atcaagccat ttaccatagc cgttctgaac aaagacaaca
    18481 tcgccaacaa taccatcaaa atggcgacca ttaaggactg ttggtgataa tgttttgaca
    18541 aattgttgaa gtgagttcat cgtgtttctc ctaaaattgt aaaataaagg aaaacacacc
    18601 gcagggagtg tttccctacg ggacataaag taaagcccaa cataaattgt cgggcttggg
    18661 taaaattact cagattgctc atctgctttt ggtttttctt gatagacgat ttccccatca
    18721 attttaatca ttttgatgcg gattaagcga cctttaagag acactccagt atcgcctttt
    18781 ttgtgatatt tactgtcact tgaataagtg aaagtatcga accacaaatc agccattaca
    18841 aatgatacaa gcactttttt ctcttcagag accgcttttt ggcatttttt gatgagtgtt
    18901 tcggtttctt taccgactac attcatatca aaatatctgt actctggtga gtcagattcg
    18961 ccaacaagag ctgcaacacg acaagcataa aaaggatttc cttttttatg ctctactaaa
    19021 cgaatatcgt ttaaatagcc aatgccggta gtatgtagat tgaagtaatt tttttgagta
    19081 gaagtttgag ttgtcatagt ttgtttctcc aaatgtaaaa aagcggagaa acatccttac
    19141 ccaacacggg aaaaatgttc cccgcaaggt ggaagtaaaa tggaatctca atgaatattc
    19201 actgaaatga tagggattga gttggcattc ctgcaaattt acagagttcc ctttcagaat
    19261 gctgggagac attttggcaa taactcaact acctctaagg atagccgtcg cttctcagga
    19321 gttatgcgac ttattaagtg ctgttatata cagcgtctta aattgag
//
LOCUS       DANLUQ010000034        19089 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.034, whole genome shotgun sequence.
ACCESSION   DANLUQ010000034 DANLUQ010000000
VERSION     DANLUQ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 19089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19089
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.034"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            203..1138
                     /locus_tag="PXB27_000942"
     CDS             203..1138
                     /locus_tag="PXB27_000942"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03571 family LLM class oxidoreductase"
                     /protein_id="HDL5345755.1"
                     /translation="MNTLSPIFSQVFGQNQLTLGLIAPFMGYQNHIPDNLTTIADNAR
                     LAENLGFSALWVRDVPFYDPHFGDVGQGLDPMVTLGFLSAHTQNIALGTAGLIAPLRS
                     PIHIAKMATSVDSLSHGRFLLGLSSGDRPVEYPAFGADFASRSERFRESIDLINTLTE
                     QDFPHFKGKHYADLTDNLDLLPKPKHRLPIVAIGRARQEMDWLANVPDAWIWHGVNPA
                     DTANIINTLADLRQKDTPTAFGYAQFVEVLDDKNAKAQLFNNIYLRGGAKGLADFWAK
                     EQEKGVKHLALNLKPTQRPADEVLTELAEYALTQF"
     gene            1325..2383
                     /locus_tag="PXB27_000943"
     CDS             1325..2383
                     /locus_tag="PXB27_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HDL5345756.1"
                     /translation="MAYPIKSYATFSATTPLAPHSFERRELLEDDVLIDILYCGVCHS
                     DLHMARNDWGWTQSYPLVPGHEIIGRIIKVGKNAKKFQIGDLVGVGCMVDSCRECSPC
                     QHGLEQYCEVGNIGTYGGIDRFDGTPTQGGYSTCVVVSEDFVLKVSENLDTKAVAPLL
                     CAGITTYSPLRHWNVKAGSKVAVVGLGGLGHMAVKLAKAMGAEVTLFTRSLGKADDAY
                     RLGASRVVLSTDEKQMADVANTFDLIIDTVPYTHDLKPYIPTLALDGTLVLVGLVGEL
                     EQTINTVPMILGRRSISASVIGGIQETQEMLDFCAEHNIVPDVEMIDMQNINDAYERM
                     LKSDVKYRFVIDMGSLKG"
     gene            2463..3293
                     /gene="fghA"
                     /locus_tag="PXB27_000944"
     CDS             2463..3293
                     /gene="fghA"
                     /locus_tag="PXB27_000944"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014167586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HDL5345757.1"
                     /translation="MQQFENYRCFSGEQQVWQHFSASLNCEMKFAIYLPQSAKTQKLP
                     VLYWLSGLTCTEQNFITKSGFQRYADEHHLIVVAPDTSPRGVNVANDEAYDLGQGAGF
                     YLNATQGEWTTHYQMYDYIVSELPTLINAHFPTNGKQSIFGHSMGGHGALTIAFKNPG
                     QYQSVSAFAPIVAPTQVPWGQKAFLAYLGEDQTAWKAYDSVALLEKYRPSLPILIDQG
                     SQDSFLSDQLKPEIFCETADRLGIPYQFNLRDGYDHSYYFISSFIGEHIAFHAKHLKD
                     "
     gene            3546..3932
                     /locus_tag="PXB27_000945"
     CDS             3546..3932
                     /locus_tag="PXB27_000945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345758.1"
                     /translation="MTNQKFINSSLTLAFFTLISFSTAYCYAWGIAAFHGYPWWHVEV
                     GNAGIARALAYVFGTFLTIFLFYLIGYALVNKVFKLHYFKYLGWLRVSVLVTIFSLPV
                     MISFYLFIGKVPMYLHLLYLGTTTSI"
     gene            4007..4276
                     /locus_tag="PXB27_000946"
     CDS             4007..4276
                     /locus_tag="PXB27_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345759.1"
                     /translation="MLSEERFGFFYIFIFIYFSLLSLSIGYIRPELRTTYDYLEIENK
                     RYYVLSIHRNNIYVLGEKTKDNDEFLFFNQDTLKYYRINITKMPN"
     gene            complement(4330..5475)
                     /gene="lldD"
                     /locus_tag="PXB27_000947"
     CDS             complement(4330..5475)
                     /gene="lldD"
                     /locus_tag="PXB27_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent L-lactate dehydrogenase LldD"
                     /protein_id="HDL5345760.1"
                     /translation="MIISSANDYRRAAKRRVPPFMFHYADGGSYAEQTLRRNVSDLED
                     IALRQRVLKDMSQLDTGIELFGEKLSMPVTLAPVGALGMYARRGEVQAAKAADNKGIP
                     FTLSTVSICPIEEVAPAIKRPMWFQLYVLKDRGFMKNALERAKAAGCSTLVFTVDMPT
                     PGARYRDMHSGMSGPYKDIRRVLQAMVHPFWAWDVGIHGKPHTLGNVSNYMGKPIDLN
                     NYIGWLTDNFDPSISWKDLEWIREFWDGPMVIKGILDPEDAKDAVRFGADGIIVSNHG
                     GRQLDGVLSSAKALPSIADAVKGEIKILADSGIRNGLDVVRMLALGADCTMIGRSFVY
                     ALSAAGQAGVENLLDIFLKEMKVAMTLTSNAKISDIGRDALVDLSNL"
     gene            6087..6731
                     /gene="rppH"
                     /locus_tag="PXB27_000948"
     CDS             6087..6731
                     /gene="rppH"
                     /locus_tag="PXB27_000948"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HDL5345761.1"
                     /translation="MIDFDGYRPNVGIVICNKHGQVLWAKRFGQNSWQFPQGGINEGE
                     NIEAAMYRELYEEVGLSKKDVRLLWASKYWLKYKLPKRLVRNEGSGPVCIGQKQRWFL
                     LQLIGDESLINLKTTKNPEFDGWRWVSFWYPVRQVVSFKRDVYRKAMKEFAQVLQNNI
                     QKATDDKTGEKPRIERGETSPHTYKKTSQDSAKAYKREARKPYRPWHQHNQKGK"
     gene            6733..7521
                     /locus_tag="PXB27_000949"
     CDS             6733..7521
                     /locus_tag="PXB27_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDL5345762.1"
                     /translation="MTILLSCLFLGVFVGFLAGLFGIGGGLIIVPSLVFLLPMAGVPP
                     EYIMSTALGTSFSTIIITAFASAQRHHKLGNVDIQTSKYFIPALMVSVFLSGLVVSRL
                     DPQLMSRIFAVMVLYLAGKMIFSIKRAPKNKPLTVQTTIIGGSIIGALASMAGIAGGA
                     FIVPFLNDRGLEMKRAIGTSSFCGAFLGLSGTISFIVSGWGLEGMPDYSLGYVYLPAL
                     FGITATSFFTSKMGASAANALPVPMLKKAFAVLLVCIAINMFLK"
     gene            7529..8326
                     /gene="lgt"
                     /locus_tag="PXB27_000950"
     CDS             7529..8326
                     /gene="lgt"
                     /locus_tag="PXB27_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDL5345763.1"
                     /translation="MNEQFIQFPQISPIVFEIGPIALRWYGLMYLAGFAFAYWLGMRR
                     AKNSNGVWTTEQVDSLVYTCFWGVVLGGRIGDVFFYSFDRFLQDPLYLFRIWEGGMSF
                     HGGLIGVIVAMIWVSYRQKRNFWQTADFIAPLVPFGLGMGRIGNFINDELWGRVTDVP
                     WAVLFPSGGYLPRHPSQLYEAFLEGVVLLFILNWFIQKPRPAGAVSGLFLIGYGTFRF
                     IVEYFREIDHNVNTTADLITRGQLLSLPMIIGGILIMAWAYKVNKVK"
     gene            8413..9081
                     /locus_tag="PXB27_000951"
     CDS             8413..9081
                     /locus_tag="PXB27_000951"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HDL5345764.1"
                     /translation="MSKKYKVIGFDLDGTLINTLPDLTLVANSMFLEAGLPTVTQEKV
                     LTWIGKGADIFFQNAVAHTGRIFQADELVKMRVSFDKYYATYVCEESTLFPNVKETLE
                     ILKANDYPLVVITNKPTKLVEPVLSAFGIYHLFDETLGGQSLPKIKPHPDPMFFICEK
                     FGITPSELLFVGDSENDVIAAKAAGCDVVGLTYGYNYNVPIEQSNPTFVTSEFKDVLK
                     IVGL"
     gene            9151..9411
                     /locus_tag="PXB27_000952"
     CDS             9151..9411
                     /locus_tag="PXB27_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HDL5345765.1"
                     /translation="MPTIALRQVGGSVAMVIPPAFLEALKLSVGSKVDLSLNDNQLVI
                     NPARPKVKYSLEELLRQSDFEAARNEESQSWLSEPAIGKEIL"
     gene            9408..9740
                     /locus_tag="PXB27_000953"
     CDS             9408..9740
                     /locus_tag="PXB27_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL5345766.1"
                     /translation="MKRGDIYWLDLDPTLGHEQSGFRPVVIVSATAFNLASKLPVVVP
                     ITNGGAFAERLGFAVSLKDAGLKTTGIIRCDQPRTVDIAQRHGKFIEALPESLLDEIM
                     ARVSVIFE"
     gene            9867..10898
                     /gene="trpS"
                     /locus_tag="PXB27_000954"
     CDS             9867..10898
                     /gene="trpS"
                     /locus_tag="PXB27_000954"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDL5345767.1"
                     /translation="MSKPIVFSGVQPSGELTIGNYLGALRNWVKMQDDYDCVYCIVDQ
                     HAITVRQDPESLRKSTLDVLALYLACGIDPNKSTIFIQSHVPEHAQLAWVLNCYTYFG
                     EMGRMTQFKDKSARYEENVNVGLFTYPVLMAADILLYQANQVPVGDDQKQHLEITRDI
                     ANRFNALYGKKDSEGNLIEPVFAVPEVFIAKSGARVMSLLEPTKKMSKSDDNRNNVIG
                     LLEDPKAVAKKIKRAMTDGDEPPVVRYDVQNKAGVSNLLDILSAITGKTIAELETEFE
                     GKMYGHLKTEVADKVSELLTNLQERYHHYRQDEALLEKIYREGAEKARAQAKATLDKV
                     YELVGFVRY"
     gene            complement(10962..11717)
                     /locus_tag="PXB27_000955"
     CDS             complement(10962..11717)
                     /locus_tag="PXB27_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HDL5345768.1"
                     /translation="MKFIKKLSYSVFLATVITACATTESINQEAAQSYAQVKTQARQQ
                     NAIDNSSATAKRIHTVFHKMKPYAERANQTGVPFHWEITVLKSDELNAWAMPGGKMAF
                     YTGLVERLNLSNDEIAVVMGHEMAHALKEHGKSDRTVSAITSIVGAIADVAVTASTGV
                     STEGLLSTGVDLIATKPFSRSQETEADEVGLILMAESGYNPSAAPNVWVKMSKANGDS
                     GLSIFSTHPSNADRQENLARLVPEAMKIYNARK"
     gene            complement(11773..12300)
                     /gene="ppa"
                     /locus_tag="PXB27_000956"
     CDS             complement(11773..12300)
                     /gene="ppa"
                     /locus_tag="PXB27_000956"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HDL5345769.1"
                     /translation="MGLELVPAGKELPDDIYVVIEIPANSDPIKYEVDKETGALFVDR
                     FMATAMFYPANYGYVNNTLSSDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGG
                     DAKVVAVPHTKLSKEYDHIKDVGDLPALLKAQIQHFFESYKALEAGKWVKVEGWGDVN
                     EARQEILESFERAKK"
     gene            12517..12592
                     /locus_tag="PXB27_000957"
     tRNA            12517..12592
                     /locus_tag="PXB27_000957"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12550..12552,aa:Lys,seq:ttt)
     gene            12615..12690
                     /locus_tag="PXB27_000958"
     tRNA            12615..12690
                     /locus_tag="PXB27_000958"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12648..12650,aa:Lys,seq:ttt)
     gene            12712..12787
                     /locus_tag="PXB27_000959"
     tRNA            12712..12787
                     /locus_tag="PXB27_000959"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12745..12747,aa:Lys,seq:ttt)
     gene            12880..13914
                     /gene="mltG"
                     /locus_tag="PXB27_000960"
     CDS             12880..13914
                     /gene="mltG"
                     /locus_tag="PXB27_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HDL5345770.1"
                     /translation="MLKKILIALGLAGVIACGGLFYGYQKLTSLAEHPITVQPNQLFV
                     LEKGVSSQKLAALLEEQGIVTHDDADLIPYLMRLYPELSKFKAGAYSLAGLTTVKDLL
                     AHLSSGKEVQLNVQFIEGKTFKIWREQLAKADYLQHTLQGKSEKEIADLLGIPHEKIE
                     GWIAPDTYNYTPNSTDLALLKRAYQKQKTALEQAWKNRAENLPLATPYEMLILASIVE
                     KETGIATERPQVASVFINRLRNNMRLQTDPTVIYGMGDRYNGNIRRKDLEEATPYNTY
                     QIDGLPPTPIAMPSEASIKAVANPDNTPYLYFVADGTGGHKFSKTLNEHNKAVAEWIQ
                     IERKRKNEQK"
     gene            13925..14545
                     /locus_tag="PXB27_000961"
     CDS             13925..14545
                     /locus_tag="PXB27_000961"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDL5345771.1"
                     /translation="MRGKFIVIEGLEGAGKTTAHQVILKELEQAGINDVVMTREPGGT
                     PLAEKLRHLIKHETEEAINDKAELLMLYAARVQLVENVIKPALAEGKWVLGDRHDMSS
                     QAYQGGGRKLDRHLLETLKESVLGTFEPDLTIYLDLDPVIGLERARGRGELDRIEQQS
                     LDFFYRTRERYLELTQHNDKAVIINAEQPIEQVTADIQQVIRNWLK"
     gene            14545..15522
                     /locus_tag="PXB27_000962"
     CDS             14545..15522
                     /locus_tag="PXB27_000962"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDL5345772.1"
                     /translation="MYPWLESTYQQLTDSFLQGRGHHALLFKTDVGLGTDTLIYKVAE
                     WLLCSQKLRHQPCLECKSCLLMQSNNHPDFHILEPIEDKDIGIDQVRELIYKLQNFAQ
                     QGGNTVVYIRGVDRLTEASSNALLKTLEEPRENVYFLLEAPLQATVLATIQSRSQTWV
                     LYAPQASEVMAWLQTQFPAISTDDLEIALRLCHSRPLICKNFIENDRLQQRKTFLQTF
                     WRFYKSHDVFLLFQAFAQEKELILQQLEWLESFFSDALKAKMAISHGWLNPDLQNGIL
                     PFSQQLTTHGLLKGHQILQQTQRDLTEINAVNPELMLLDCLTKLVLVFE"
     gene            15653..15895
                     /locus_tag="PXB27_000963"
     CDS             15653..15895
                     /locus_tag="PXB27_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HDL5345773.1"
                     /translation="MARTTSVTLGEPLNDFVNEMVASGRYGSTSEVVRTALRMLEEKE
                     QYLAQLRQAIDDGDASGYATETIDEMMLRVKAELNV"
     gene            15888..16172
                     /locus_tag="PXB27_000964"
     CDS             15888..16172
                     /locus_tag="PXB27_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5345774.1"
                     /translation="MYKLSAQALRDQEKLFRYSISQFGVQQTESYFADLKHILNLLAD
                     FPTMGTSAEYIRGGLRMHTHQSHTIYYRIKADHIFIVRILHNQMNHKRYI"
     gene            16177..16965
                     /locus_tag="PXB27_000965"
     CDS             16177..16965
                     /locus_tag="PXB27_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchF/TatD family DNA exonuclease"
                     /protein_id="HDL5345775.1"
                     /translation="MKDLFIIDSHCHLDSLDYETRHKNVDEVIENAKARGVHHFISVC
                     TTVGRFEAMKNLTAHRNDVSLSCGVHPLNVEDEPFDYDKLFQFAQDPKVVAIGETGLD
                     YHYTPETKALQQSLFVQQIEIANKLNKPLIIHSRSARQDTMDMLEQNNAEKCGGVLHC
                     FTEDWAMAKRALDIGFYISISGIITFRNAEELREVVRKVPLDRLLVETDSPYLAPIPY
                     RGKPNQPAYVRETCEYVATLKGVSTEELARITTENVQHLFNIKL"
     gene            16975..17496
                     /locus_tag="PXB27_000966"
     CDS             16975..17496
                     /locus_tag="PXB27_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1523 family protein"
                     /protein_id="HDL5345776.1"
                     /translation="MRAILKYFLILVSLSFFIVLGGAVNYAMPSYEDTVVTGMEVRRM
                     DKDGIISKANPADGEVRDVYFLFTEDPETKKVMVYRNEDTGWGLPPYFKFGSADIQAK
                     AQAYANEKQRVQIKYYGWRINWLNEFRNIVSIKPLEEAETVSKPIMSYVLYAVLAFLF
                     FLSVQFIRGIFKD"
     gene            17571..18272
                     /gene="lpxH"
                     /locus_tag="PXB27_000967"
     CDS             17571..18272
                     /gene="lpxH"
                     /locus_tag="PXB27_000967"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HDL5345777.1"
                     /translation="MTYYFIADLHLNENQPEITHLFLQFMQEKALLAEAVYILGDLFD
                     FWIGDDEQSELISTVKQAIKTLTTSGTKCYFICGNRDFLIGKRFSEQTGMVILPDYHV
                     IDLFGTSTLLCHGDTLCTDDVKYQQFRKKVHQKWRQAIFLMFPLSWRIRIAEKIRSKS
                     KQEKRDKSAEIMDVNAEVTAQIVAKYNTTQLIHGHTHRQAIHQHNGFTRIVLGDWKEN
                     YASILSVTEQGAEFI"
     gene            18269..19059
                     /locus_tag="PXB27_000968"
                     /pseudo
     CDS             18269..19059
                     /locus_tag="PXB27_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618283.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
BASE COUNT         5582 a         4095 c         3978 g         5434 t
ORIGIN      
        1 tgttttttcg gaacgtttta tttataataa gccttatgct cacttaagtt tgaaatattt
       61 attataaatt ttgcaaattt tcagtcagct tgaaagcgtc tatttatgcc aatttagcaa
      121 aatactttct caatttatcc cattttaagc aaactcaaaa gcccttaaaa taccttatct
      181 taaataagac aggtacgcca aaatgaacac gctttccccc attttctccc aagtttttgg
      241 gcaaaatcaa ttaacactgg gcttaattgc cccctttatg ggctatcaaa atcacattcc
      301 agacaatttg accaccattg ccgacaatgc acggctggct gaaaacttgg gcttttcagc
      361 attgtgggta cgagatgtgc cgttttatga ccctcatttt ggcgatgtgg ggcaagggct
      421 tgacccgatg gttacacttg gttttttgtc cgcccacacc caaaacatcg cccttggcac
      481 ggcagggctg attgcccctt tgcgtagtcc cattcacatt gccaaaatgg cgacaagtgt
      541 ggatagtttg agtcatggac ggtttttatt gggcttatcc tctggcgata gacctgtgga
      601 ataccctgca tttggggcgg attttgcctc tcgtagcgag cgttttcgtg aaagcattga
      661 tttgattaac accttaaccg agcaggattt tccccatttc aaaggcaaac attatgccga
      721 tttaacagac aatttggatt tgctccccaa acccaaacac cgcttgccca ttgtggcgat
      781 tggacgagca aggcaagaaa tggactggct tgctaatgta cctgatgcgt ggatttggca
      841 cggtgtgaat cctgccgaca ccgcaaacat catcaacacc cttgccgatt tacgccaaaa
      901 agacaccccc acagcatttg gctatgctca atttgtggaa gttttggacg acaaaaacgc
      961 caaagcacag ttatttaaca acatttatct gcgtggtggt gcaaaaggct tggcagactt
     1021 ttgggcaaaa gaacaagaaa aaggcgtgaa acatttggca ctcaatttaa aacccactca
     1081 gcgtcctgct gatgaagtgc ttacagaatt ggcagagtat gctttgacac aattttgatg
     1141 aaaaacaccg cttttgtgat gagggcggtg tttttgtttt taaaatgggc aaaataattt
     1201 ttcagtcagt ctgaaaaccc ccaattatca ttttaactga taccgattta tcaaaaatct
     1261 tgattttcaa taataaatca tcggcactat agtaattgcc atttcagcca tttaaacccc
     1321 aaaaatggct taccctatca aatcctacgc cactttttcg gcaaccacac cacttgcccc
     1381 acacagtttt gaacgccgtg aattgcttga agacgatgtt ttgattgata ttttgtactg
     1441 tggcgtgtgc cattcagact tgcatatggc tcgcaatgat tggggctgga cgcaaagcta
     1501 tccgcttgta ccagggcacg aaatcatcgg tcgtattatc aaagtcggta aaaacgccaa
     1561 aaaattccaa atcggcgatt tggtcggtgt cggttgtatg gtggattcct gccgtgaatg
     1621 cagtccttgc cagcacggtt tggagcagta ctgcgaagtg ggcaacatcg gcacttatgg
     1681 cggtattgac cgctttgacg gcacaccaac acaaggcggt tacagcacat gcgtggtggt
     1741 gagtgaagat tttgtattaa aagtctctga aaacttggac accaaagcgg ttgcccctct
     1801 tttgtgtgca ggcattacca cctattcgcc cctgcgtcat tggaatgtca aagctggctc
     1861 aaaagtcgct gttgtggggc ttggcggact ggggcatatg gcggttaaac ttgccaaagc
     1921 aatgggagcg gaagttacgc tttttactcg ctcactgggt aaagccgatg atgcttaccg
     1981 tttgggcgca agccgtgtgg tgctttctac cgatgaaaag caaatggcag acgttgcaaa
     2041 tacctttgat ttaatcatcg ataccgtgcc ttatacccac gacttgaaac cttatatccc
     2101 aaccttagcg ttggacggca cattggtgct ggtgggcttg gtcggtgagt tggagcaaac
     2161 catcaacacc gtaccgatga ttttaggccg ccgctctatt tccgcctctg tcatcggcgg
     2221 cattcaggaa actcaagaaa tgctcgactt ctgcgccgaa cacaacattg tgccggatgt
     2281 ggaaatgatt gatatgcaaa acatcaatga tgcttatgaa cgaatgctga aatcagacgt
     2341 gaaatatcgg tttgtgattg atatgggaag tttgaaaggt tgatattaca atataaaggc
     2401 ggtgagttcc gcctttattt ttgcaaaaaa tcagcggaat taaaccgctt gtaaggaata
     2461 ttatgcaaca atttgaaaac taccgctgtt ttagcggtga acaacaggtt tggcaacatt
     2521 tctcagccag cttaaactgt gaaatgaaat ttgctattta tctgccgcaa tctgctaaaa
     2581 cacaaaagct acccgtacta tattggcttt ctggtttaac ctgtaccgag cagaacttca
     2641 tcactaaatc tggctttcaa cgttatgccg acgaacatca tctgattgtg gtcgcaccgg
     2701 ataccagccc taggggtgtg aatgtcgcta atgatgaggc ttatgattta ggtcagggtg
     2761 ccggatttta cctaaatgct acgcaaggcg aatggacaac acattaccaa atgtatgatt
     2821 acattgtcag cgaactgcca acattgatta atgctcattt cccgactaac ggtaaacaat
     2881 caatttttgg gcattcaatg ggcggacatg gtgccttaac cattgcattt aaaaatccgg
     2941 ggcaatatca aagtgtatcg gcatttgcac cgattgtcgc cccaacccaa gttccttggg
     3001 ggcaaaaagc atttttagca tatttaggcg aagatcagac cgcttggaag gcgtatgata
     3061 gcgtggcatt gctggaaaaa tatcgcccaa gcttgccaat tctaatcgat caaggcagcc
     3121 aagacagctt tttgagcgat cagttaaagc cggaaatttt ctgtgaaacc gcagatagac
     3181 ttggaatacc ctaccaattc aatttacgtg atggttacga tcacagctac tatttcatta
     3241 gtagctttat cggtgaacat atagcctttc atgcaaaaca tttaaaagac taaagacagt
     3301 aaattatcga aacatgcttt atcattttta tcatgtttcg atatattcag agtgcattac
     3361 aagcggtcag tttacgatca ttttttacaa cacggttgtt ttgtataaaa acaaaggttt
     3421 gaaatacaac aaaagtcgtg ctttaataaa gttctcctct aaacaaagga gttgggtgga
     3481 agctaaactc agtctgcttt cttgtgtttc atctttaact aagtaggtgc aatctattta
     3541 ctgttatgac taatcagaaa ttcatcaact cttctcttac tctcgctttc tttactttaa
     3601 tctctttttc taccgcttac tgttatgctt ggggtattgc tgcttttcat ggctacccgt
     3661 ggtggcatgt tgaggttggt aatgccggta tagctcgcgc actagcctac gtttttggca
     3721 catttttaac tatctttctt ttttatttga taggatacgc tttagttaat aaagttttta
     3781 agctgcatta ctttaaatac cttggctggc tacgggtgag tgttcttgtt actattttta
     3841 gcttgccagt aatgatcagt ttctatctgt ttattggcaa agtgccaatg tatttacacc
     3901 tactctatct agggacaact acctctatct agggacaact accctatcag tattattatt
     3961 tcacaaacat tgggaccacc gcgtattcca cttagatatt caaaaaatgt taagcgaaga
     4021 acgcttcggt tttttctata tttttatttt catctatttt agcttacttt ctttgagtat
     4081 tggctacatt cgccctgagc tgagaacaac gtatgattat cttgaaattg aaaataagcg
     4141 atattatgtc ttatctattc atcgcaataa catttatgtg ctgggcgaaa aaactaaaga
     4201 taacgatgaa tttctctttt tcaaccaaga tacattgaaa tattaccgca ttaatattac
     4261 caaaatgcca aattagccta tctaaaataa caatccgcac ctaaaacgtg cggattgtgg
     4321 ctatttgagc taaagattac ttaaatctac caaagcatct ctgccgatgt cgctaatttt
     4381 agcattgctg gtaagtgtca tcgccacttt catttcttta aggaagatgt cgagcaggtt
     4441 ttccacaccg gcttgaccgg cggcgctaag agcgtaaaca aaggaacgcc cgatcatcgt
     4501 acaatctgcg ccgagagcca gcattctcac cacatctaag ccgttgcgga taccagagtc
     4561 ggcaaggatt ttgatctccc cttttactgc atcggcaatg cttggcaggg ctttggcaga
     4621 agaaagtacc ccatctaact gacgaccacc gtggttggag acgatgatac cgtccgcacc
     4681 aaatcttacg gcatctttag catcttctgg gtcgagaatg cctttgatca ccatcgggcc
     4741 gtcccaaaat tcacgaatcc actctaaatc tttccacgaa attgatgggt cgaaattatc
     4801 ggtcagccag ccgatgtagt tgttcagatc aatcggttta cccatatagt ttgacacatt
     4861 gcctaacgtg tggggtttac cgtgaatgcc cacgtcccac gcccaaaacg ggtgaaccat
     4921 tgcctgcaac acacggcgaa tgtctttgta tgggccgctc attccggagt gcatatcacg
     4981 ataacgtgcg ccgggggtcg gcatatctac ggtaaacact agcgtggaac aaccggcggc
     5041 tttggctcgt tccaatgcat ttttcataaa gccccgatct tttaacacat agagctgaaa
     5101 ccacatcggg cgtttgattg ccggtgcaac ctcttcaatc gggcaaatag agacagtgga
     5161 aagggtaaac ggaatacctt tgttatctgc ggctttggcc gcttgcactt cgccacggcg
     5221 ggcatacatt cctaatgcac cgacaggggc gagggtcacc ggcatagaca gtttttcgcc
     5281 gaacagctcg atgccggtgt ctagttgcga catatctttt aacacacgtt gacgcagggc
     5341 gatgtcttcc aaatcgctca cattacgacg taaggtctgc tcggcgtaag aaccgccatc
     5401 cgcataatgg aacataaaag ggggaacacg acgttttgcc gcacggcggt aatcgtttgc
     5461 agatgaaatg atcatttaaa acctcataaa tcattaagaa aaaagtgcgg ctattctact
     5521 ttaaaataaa aataagaatt actactattt gcaaaatatg agaaatagat cacatttttg
     5581 ttacaaacca attagcataa aaatacaatt gcacaaaaag caaccaacaa aacaattgat
     5641 tattcacaga ttgaaaagat gacactaact atttgattta attataattt atggaaatag
     5701 caaaaggctg atttgagtta ttcacgccta atttgaaaaa ctatcacatc tttatccaca
     5761 aatggtgggg ataactctgc tttatttgcg aagcaaaaat tgccctgctt attcatttta
     5821 aaatttaaac ttattttgac gagaaaaaac gcaacaactt gatctttatc cagatcattg
     5881 cttaagaaaa agatcgcatt tttagatctg attaattgct ctgttttaca acgttccgcc
     5941 tgtttatcct caacttatac cacttatatt caatagttat ccacaaagtt attcacaagc
     6001 ggttatattt tatggttttt ttgcaaatca acccaaagta tgaaacaatt ctcccattct
     6061 agaattttta tcaattaggt ggtcgagtga tcgatttcga tggctaccgc ccaaatgttg
     6121 gaatcgttat ctgtaataag cacgggcaag ttctgtgggc aaaacggttt ggtcaaaact
     6181 cttggcaatt tccgcaaggt ggaattaatg agggggaaaa cattgaagca gcaatgtacc
     6241 gtgagcttta tgaagaggtc ggcttaagta aaaaagatgt acgattgctt tgggcgtcta
     6301 aatattggct aaaatataaa ctgccaaaac gcttggtacg taatgaaggc tctggtcctg
     6361 tctgtatcgg gcaaaaacag cgttggtttc tactgcaatt aatcggtgat gaaagcctga
     6421 ttaatttgaa aaccacgaaa aatccggagt ttgacggctg gcgttgggtc agcttttggt
     6481 atccagtccg ccaagtggtc tcttttaaac gagatgttta tcgcaaagcg atgaaagagt
     6541 ttgcacaggt tttgcaaaac aatattcaaa aagcgactga cgataaaacc ggtgagaaac
     6601 cacgcattga gcgtggcgag acttcgcccc atacttataa aaaaacctct caggattctg
     6661 ccaaagccta taaacgagaa gctcgtaagc cttatcgtcc ttggcatcaa cataatcaaa
     6721 aaggaaaata agatgactat tttattaagc tgcctttttt taggggtgtt tgtcggcttt
     6781 ttagccggct tgttcggcat tggtggtggg ttaatcatcg tgccgagttt ggtcttccta
     6841 ctgcccatgg caggtgtacc gccggaatat ataatgtcca ccgcactcgg cacatcattt
     6901 tcaaccatta ttattaccgc ctttgcatcg gcacaacgcc atcataagtt aggcaatgtc
     6961 gatatccaaa ccagtaaata ttttattcca gcgttgatgg tttcggtttt tctatcgggc
     7021 ttggtggtaa gccgcctcga tccgcaactg atgtctagaa tttttgcggt aatggtactt
     7081 taccttgcag gtaaaatgat tttttcaata aaaagagcac cgaaaaataa accacttacc
     7141 gttcaaacga cgataatcgg aggttcaatc atcggtgcgt tagccagtat ggcagggatc
     7201 gcaggtggag cctttattgt accattttta aacgatcgag gcttagaaat gaaacgtgcc
     7261 atcggcactt cctcattttg cggagcattt ttaggtttat ctggcacaat cagctttatt
     7321 gtaagtggct ggggcttaga aggaatgcct gattattctc tcggttatgt ttatttaccg
     7381 gctctgtttg gtattacggc aacttcattt ttcacctcaa aaatgggggc aagtgccgcc
     7441 aacgctctac ctgtgccgat gctgaaaaaa gcctttgcgg tgcttttggt ctgtatcgcc
     7501 atcaatatgt ttttaaaata aggacactat gaacgaacaa tttatccaat ttccacaaat
     7561 tagcccgatt gtgtttgaaa tcggtccgat tgccctgcgt tggtatgggc taatgtattt
     7621 agccggcttt gcctttgcct actggctggg aatgagacgg gcgaaaaatt ccaatggagt
     7681 ttggacaacc gagcaagtgg atagcttagt ctatacttgc ttctggggcg tagtattagg
     7741 tgggcgtatc ggcgatgtgt tcttttatag cttcgaccgt ttcttacaag atccgctcta
     7801 cctctttcgc atttgggaag gcggaatgtc ctttcacggc ggtttaatcg gcgtcattgt
     7861 agcaatgatt tgggtttcct accgccaaaa acgcaatttc tggcagacag cagattttat
     7921 cgcaccgcta gtaccgttcg ggctaggtat ggggcgtatc ggcaatttca ttaacgatga
     7981 actttggggg cgtgtaaccg atgtgccttg ggcggtgtta tttccaagcg gtggctatct
     8041 tccccgccac ccgtcacagc tttatgaagc ctttttagag ggtgtggtgt tactctttat
     8101 cctcaactgg tttattcaaa agcctcgccc ggcaggtgcg gtttcagggt tatttttaat
     8161 cggctacggc acattccgct ttattgtgga atacttccga gaaatcgacc ataacgtcaa
     8221 taccaccgca gatttaatca ctcgtgggca actactctct ttaccgatga ttatcggcgg
     8281 catactgatt atggcgtggg cgtataaagt aaacaaagtg aaataaacgt aggggcaagt
     8341 caatatttgc cctcattaca aaaattgcaa aaaatttgca gaaaacgacc gcttgtgttt
     8401 gaaggatata ctatgagtaa aaaatacaaa gtcatcggtt tcgacttaga cggaacgcta
     8461 atcaacaccc tccccgatct cactcttgtc gccaattcaa tgtttctaga agccggtttg
     8521 ccaacggtta cacaagaaaa agtgctgact tggatcggta aaggggcaga tattttcttc
     8581 caaaatgcgg ttgctcacac cggcagaatt ttccaagccg atgagttggt gaaaatgcga
     8641 gtgagctttg ataaatacta cgccacctat gtgtgcgagg aaagcaccct tttcccaaat
     8701 gtgaaagaaa ccttagaaat cctcaaagca aacgactacc cgctggtggt gattaccaac
     8761 aaaccgacca aattagtcga gccggtgctt tccgctttcg gcatttatca tttattcgat
     8821 gaaacccttg gtgggcaatc gctccccaaa atcaaaccgc accctgaccc gatgtttttt
     8881 atctgcgaaa aattcggcat taccccatca gaattacttt ttgtcggcga ctcagaaaat
     8941 gacgtgattg ccgccaaagc cgccggttgt gatgtggttg gactaactta cggctataac
     9001 tacaatgtgc cgattgaaca atctaacccg acttttgtga catctgaatt taaagatgtg
     9061 ttgaaaattg tggggttata aagaaaacat ttgttttctt ttgatgtttg ctttagactt
     9121 tccgaaccta aagatttaaa ggagtaaagt atgccaacaa tcgcattacg tcaggtcggt
     9181 ggctcggttg caatggtgat tccgcctgct tttttagagg cactcaagct ctccgtcggt
     9241 agcaaagtgg atttgtcttt aaacgacaac caactcgtga ttaatcctgc ccgcccaaaa
     9301 gtgaaataca gcttagagga attattaaga caatccgatt ttgaggcagc aaggaatgaa
     9361 gaaagccaat cgtggttatc cgagccggca ataggaaaag aaattttatg aaacgtggcg
     9421 atatttactg gctcgatctt gatccaacct taggacacga acaatcaggc ttccgccccg
     9481 ttgtaattgt ttccgcaact gcatttaatc tagccagtaa actgcctgtg gttgtgccga
     9541 ttactaacgg tggagctttt gctgaaagac tgggctttgc cgtttcatta aaagatgccg
     9601 gcttaaaaac aacagggatt attcgatgcg atcaacctcg cacagtcgat attgcacaac
     9661 gccatggaaa atttatcgaa gccttacccg aaagcctact tgatgaaatt atggcaaggg
     9721 tttcagtgat ttttgagtaa gatacaagcg gtctgatttg ctctattttt tgcaaaatca
     9781 tcaataaatc tgaccgcttg tacttttatc ccttcccaat aaccgttaag ataacctata
     9841 tttttcaaat caaacgagaa gataaaatga gcaaacctat tgtatttagt ggcgtgcagc
     9901 cctcaggtga attgactatc ggtaactact taggggcgtt acgcaactgg gtgaaaatgc
     9961 aagatgatta tgactgtgta tattgcattg tcgatcaaca cgccattacc gttcgtcaag
    10021 atccggagtc gctacgcaaa tcaactttag atgtgttagc cctgtattta gcttgtggca
    10081 ttgatccaaa caaaagcact atttttattc aatcccacgt gccggaacac gcccaattag
    10141 cttgggtgct gaactgttac acctatttcg gtgaaatggg gcgtatgacg caatttaaag
    10201 ataaatcggc acgctatgaa gagaatgtca atgtgggctt atttacttat ccggtattaa
    10261 tggcggcaga tattctgctt taccaagcca accaagtgcc ggtaggcgat gaccaaaaac
    10321 aacatttgga aatcacccgt gacattgcca accgtttcaa tgccttatac ggcaaaaaag
    10381 attccgaagg caacctgatc gagcctgttt ttgcagtgcc ggaagtcttt attgcgaaat
    10441 ccggggcaag agtgatgtcg ctacttgagc cgaccaaaaa aatgtcaaaa tcggacgata
    10501 atcgtaacaa tgtgattgga ctattggaag atccgaaagc ggtagcgaag aaaatcaaac
    10561 gggcaatgac cgatggcgat gaaccgccag tagtgcgtta tgacgtacag aataaagccg
    10621 gtgtctcgaa tttattggat attctctccg ccattaccgg caaaaccatt gccgaattag
    10681 aaaccgaatt tgaaggcaaa atgtatggtc atttgaaaac agaagtagca gataaggttt
    10741 ccgaactatt aaccaatctg caagaacgtt atcaccacta ccgccaagat gaagcattat
    10801 tggaaaaaat ctaccgtgaa ggggcagaaa aagctcgagc tcaagccaaa gcaacgttag
    10861 ataaggtata tgaattagtc ggttttgtgc gttactaaca cttaatgaaa aatgctcttg
    10921 aggattcaag agcatttttg caaaaaagaa ccgattgtca attatttacg ggcgttataa
    10981 atcttcattg cttccggcac aaggcgggct aagttttctt gacggtcagc atttgatggg
    11041 tgagttgaga aaatggacaa gcctgaatcg ccattggctt tactcatttt tacccaaaca
    11101 ttcggcgctg ctgatgggtt gtagcctgat tctgccatta aaattaaccc aacttcgtcc
    11161 gcctcagtct cttggctgcg agagaatggc ttggtggcaa tcaaatccac gccagtgctg
    11221 agtaaacctt cggtgcttac gcctgttgag gcagtcacag ccacatctgc aatagcacct
    11281 acaatactgg taatagcgct aaccgtacgg tctgatttac cgtgttcttt cagtgcgtga
    11341 gccatctcgt gtcccattac cactgcaatt tcatcgttcg ataaattaag tctttccact
    11401 aaccccgtgt aaaacgccat tttaccgccc ggcatcgccc acgcattgag ttcatccgat
    11461 ttcaatacgg taatttccca atgaaaaggt acaccagtct ggttggctct ttccgcataa
    11521 ggcttcattt tatgaaaaac agtgtgaatc cgcttagcgg tggcagacga attgtcaatc
    11581 gcattttgct gacgagcttg ggttttcact tgagcataac tttgagccgc ttcttggtta
    11641 atgctttctg ttgtggcaca agcggtaatc actgttgcta aaaaaactga ataacttaat
    11701 tttttgatga atttcatttt atatcccaca aaaaatgcca aacaaatttg cttggcattc
    11761 aaaaataaat aattatttct tagcacgctc gaatgattct aagatttctt ggcgagcttc
    11821 gtttacatcg ccccaacctt ctactttcac ccatttgccc gcttctaatg ctttatagct
    11881 ttcgaagaag tgctggattt gtgcttttaa taacgccggt aagtcaccta catctttgat
    11941 gtggtcatac tctttactta atttggtgtg tggaacagca accacttttg catcaccgcc
    12001 ggcttcatcg gtcattttta acacaccgac aggacggcaa cgaatcaccg aacccggttg
    12061 taacggatat ggtgttggca ctaatacatc aaccgggtca ccgtctgatg ataaggtgtt
    12121 attcacataa ccatagtttg ccggatagaa cattgcggtt gccataaagc gatccacaaa
    12181 taatgccccg gtttctttgt ccacttcata tttgattgga tcagagtttg ccggaatttc
    12241 aattacaaca taaatatcgt ccggtaattc tttaccggca ggaactaatt ctaagcccat
    12301 tttggtttcc ttctataata ggtgaaataa acggcgtgat tatagcctat tttcagaaaa
    12361 agataaaagc ccgtttctct tttcattttc cgccaacaaa tccggctaat tttttgctaa
    12421 attgcttaca aaccgcacac tcaaccattt ttcgataaaa aaaacttgcc aaagttttaa
    12481 aaatccgtaa gatatgcact cgttacgcaa gattatgggt cattagctca gccggtagag
    12541 cagcggactt ttaatccgtt ggtcgaaggt tcgaatcctt catgacccac cattaatctt
    12601 caaaacacct ctaagggtca ttagctcagt cggtagagca gcggactttt aatccgttgg
    12661 tcgaaggttc gaatccttca tgacccacca ttaatttcaa aactcctctg agggtcatta
    12721 gctcagtcgg tagagcagcg gacttttaat ccgttggtcg aaggttcgaa tccttcatga
    12781 cccaccattt actcaaatcc catttgcaaa tttttctgaa aaaacaaccg ctttatagta
    12841 gaattcagtc aatttttatc acttttattt tattcatcaa tgctaaaaaa aatccttatt
    12901 gcattaggct tagccggcgt tattgcttgc ggtggactgt tttacggcta ccaaaaactc
    12961 acctctttag ccgaacaccc gatcacggtt cagccgaatc aacttttcgt attggaaaaa
    13021 ggcgtttcca gccaaaaatt agcggctctg ttagaagagc aaggcattgt cacccacgat
    13081 gacgccgatc ttatccctta tttaatgcga ttataccctg agttaagcaa attcaaggct
    13141 ggtgcttatt cactcgcagg cttaacgaca gtaaaagatt tgctcgctca tttaagctcc
    13201 ggcaaagaag tgcaacttaa cgtgcaattt attgagggca aaacttttaa aatttggcgg
    13261 gaacaactag cgaaagccga ttatttacag cacacgctac aaggcaaatc ggaaaaagaa
    13321 atcgctgact tactcggcat tccacacgaa aaaatcgaag gttggatagc cccggatact
    13381 tacaactaca cgcccaattc caccgattta gccttgctta aacgggctta tcaaaagcag
    13441 aaaacggcgt tggaacaggc ttggaaaaat agagcggaaa atctaccgct tgcaacacca
    13501 tacgaaatgc tgattcttgc ctcgattgtg gaaaaagaga ccggcattgc gaccgaacgc
    13561 ccacaagtgg catcggtctt tattaaccgt ttacgcaaca atatgcgact gcaaaccgat
    13621 ccaaccgtca tttacggaat gggcgatcgt tataacggca atatccgccg caaagattta
    13681 gaagaggcaa cgccatacaa tacttaccag attgacggct taccgccaac gccgattgcc
    13741 atgccgagtg aagcttcgat taaagcggtt gccaacccgg ataatacacc ctacctctat
    13801 tttgtggcag acggcacggg cggtcataaa ttcagcaaaa cactgaatga gcataataaa
    13861 gcggtggcag aatggattca aattgaacgc aaacgcaaaa atgaacaaaa atagtgagaa
    13921 caatatgcgt ggaaaattta ttgtaattga aggcttagag ggcgcaggca aaacgactgc
    13981 tcaccaagtg attctcaaag agttggaaca agccggcatt aatgacgtag taatgacccg
    14041 agaacccggc ggcacccccc ttgccgaaaa gttacgccat ttaattaaac acgaaaccga
    14101 agaagccatt aacgataagg ctgagctgct aatgctctac gctgctcgtg ttcaattagt
    14161 cgaaaatgtg atcaaacctg cccttgccga aggcaaatgg gttctgggcg accgccacga
    14221 tatgtcttcc caagcctatc aaggcggtgg acgtaagttg gatcgtcact tactcgaaac
    14281 cttaaaagaa tcggtactcg gcacattcga gccagattta acgatttatc tcgatctcga
    14341 tccggtgatt ggtttagaaa gagccagagg cagaggcgaa ttagaccgca tcgaacagca
    14401 aagccttgac ttcttctacc gcacacgaga gcgttattta gaattaaccc aacacaacga
    14461 taaagccgtg atcatcaatg ccgaacaacc catcgaacag gtgactgcgg atattcaaca
    14521 agtaattaga aactggctga aataatgtac ccttggctcg aaagcactta tcaacaactt
    14581 accgattcat ttttacaggg tcgaggtcat cacgccttac tgttcaaaac agatgttggt
    14641 cttggtacag acaccttaat ctacaaggtg gctgaatggt tgctttgcag ccaaaaactc
    14701 cgccatcaac cttgtcttga atgcaaaagt tgcctgctta tgcaaagcaa caaccacccc
    14761 gatttccaca ttcttgagcc gattgaagac aaagatattg gtatcgacca agtgcgtgag
    14821 ttgatttata aactccagaa tttcgcccaa caagggggaa atacggtcgt ttatattcga
    14881 ggagttgatc gtttaaccga ggcaagcagt aatgccctgc tcaaaacctt ggaagagcca
    14941 cgtgaaaacg tctatttctt gctcgaagca ccgcttcaag caacagtttt agctaccatt
    15001 caaagccgca gccaaacttg ggtactttac gccccacaag cctcagaagt aatggcttgg
    15061 ctacaaacac aatttccggc aatctcaacc gacgacttag agattgcact ccgcctttgc
    15121 cactcacgcc cactaatttg caaaaatttt atcgaaaatg accgcttgca acagcgaaaa
    15181 acctttttgc aaaccttctg gcgtttctac aaaagccacg atgtcttctt gttattccaa
    15241 gccttcgccc aagaaaaaga gctgatttta cagcaactcg aatggctgga aagtttcttt
    15301 agcgatgccc taaaagctaa aatggcaatc agccacggtt ggctaaaccc ggatctccaa
    15361 aacggcattc tcccttttag ccaacaacta acgacccacg gcttacttaa agggcatcaa
    15421 attctccaac aaacccagcg ggatttaacc gaaattaacg ccgtcaatcc ggaattgatg
    15481 ttgctagatt gcctgacgaa gttggttttg gtgtttgagt aaaagccctc tctcatagaa
    15541 ggtagctttc aattagccgt aaaaagaggc ttctagccct agaatatagt ttataacttg
    15601 caaaattatc tatctttgtt attgtatgaa taattcacac aaggagaata ttatggcaag
    15661 aacaaccagc gtgactttag gcgaaccttt aaatgatttt gtaaatgaaa tggtggcaag
    15721 tgggcgttat ggctctacca gtgaagtagt gagaaccgct ttacgaatgc ttgaggaaaa
    15781 agagcaatac ttagctcagt tgcgacaagc cattgatgat ggtgatgcta gtggttatgc
    15841 aaccgaaacg attgatgaaa tgatgttgag agtaaaggcg gaacttaatg tataaacttt
    15901 ctgctcaagc actccgagat caagagaaac tttttcgtta cagtatctct caatttggtg
    15961 ttcaacaaac agaatcttat tttgcagatc taaagcatat tttgaattta ttagctgatt
    16021 ttccaacaat gggaacttct gcggaatata ttcgtggtgg cttgcgaatg cacacacatc
    16081 aatctcatac aatttattat cgaataaaag cagatcatat atttattgtt cgcatcttac
    16141 ataaccaaat gaatcataaa cgctatattt agaaaaatga aagacttatt tatcatagac
    16201 tcacattgtc atctagattc tctagactac gaaactcgtc acaaaaacgt ggacgaagta
    16261 attgaaaatg ccaaagcccg aggggtgcat cattttatct cggtttgcac tacagttggg
    16321 cgttttgaag caatgaaaaa cttaaccgcc catcgtaatg acgtttcact ttcttgtggc
    16381 gttcacccgt tgaatgtgga agatgaacct tttgattacg ataaattatt tcagttcgct
    16441 caagatccga aagtggttgc cattggggaa actggtttgg attatcacta cacaccggaa
    16501 accaaagcgt tgcaacaatc gcttttcgtt caacaaattg aaattgcaaa caaactcaat
    16561 aagccactca ttatccattc tcgttcggca cgccaagata cgatggatat gttggaacaa
    16621 aataatgccg aaaaatgcgg tggggtgttg cattgcttta ccgaagattg ggcaatggca
    16681 aaacgggctt tagacatcgg cttttatatc tcaatttcgg gcattattac tttccgcaat
    16741 gcagaagaac tccgagaagt cgtgcgaaaa gtgccgttag atcgcttatt ggttgaaaca
    16801 gattcccctt atcttgcccc gattccttat cgtggtaagc cgaaccagcc ggcgtatgtg
    16861 cgggaaactt gcgaatatgt ggcgacatta aaaggcgttt caactgaaga attagctcgc
    16921 attacaaccg aaaatgtgca acacttattc aacattaaat tataaggaac taatatgaga
    16981 gcaatactta agtattttct gattcttgtc tcactatcat tttttattgt attaggcggt
    17041 gcagttaatt atgcaatgcc aagttatgaa gataccgtag taacaggtat ggaagtacgc
    17101 cgaatggata aagatggtat tatcagcaaa gcgaatcctg ctgatggcga ggtgcgcgat
    17161 gtttatttct tattcacgga agatcctgaa accaaaaagg taatggttta tcgtaatgaa
    17221 gataccggct gggggttgcc tccttatttc aaattcggat ctgccgacat tcaagccaaa
    17281 gctcaagcct acgctaacga aaaacaacgt gtgcaaatta aatattacgg ctggcgaatc
    17341 aattggctaa atgaatttag aaatattgtt tcaatcaagc cattagaaga agcagaaaca
    17401 gtcagcaaac caattatgag ctatgtactt tatgccgtat tggcgttctt gttcttcctc
    17461 tcagtacaat ttattcgtgg catttttaaa gattaatgcc gattaacctt tgacaaacgg
    17521 ttcgattttg taaaaaattg aaccgttttc ttttttatca acaatatcaa atgacgtact
    17581 attttattgc cgatctccac ttaaatgaaa atcagcctga aattacccac ctctttttgc
    17641 aatttatgca agaaaaagca ctacttgcag aagcggttta tatcttaggt gatctgtttg
    17701 atttctggat tggcgatgat gagcagtctg aactcatttc aaccgttaaa caggcaatca
    17761 aaaccctcac tacaagcggt acaaaatgct attttatttg cggaaatcgt gattttttaa
    17821 ttggcaaacg attcagcgaa caaacgggta tggttatttt gcctgattat cacgttatag
    17881 atttattcgg cacatctacc ttgctttgcc acggcgatac gctttgtact gatgatgtga
    17941 aatatcaaca atttcgtaaa aaagtccacc aaaaatggcg acaagctatc tttttgatgt
    18001 ttccgctttc ttggcgaatt cgtattgcgg agaaaattcg atccaaaagc aaacaagaaa
    18061 agcgtgataa atccgctgaa attatggacg taaacgctga agttaccgct caaattgtcg
    18121 caaaatacaa tacaactcaa cttatacacg ggcatacgca tcgacaagca atccatcagc
    18181 ataatggatt tacccgcatt gtattaggcg actggaaaga aaattacgcc tctattttaa
    18241 gcgtaaccga gcaaggagct gaatttatat gattaatgtg attattcctt gctataacgc
    18301 cgaaaacacc cttgttcgtg cagtgcaaag tgtattaaat cagtctggat taaatctatt
    18361 gtggcttatt gatgatgctt caacggataa tacgcttgcc ttagctaaac atttacaagc
    18421 ccaagtgcct gataaaatta gagtggaaca aatgcctcaa aatggcggtg ttgccaaagc
    18481 acgcaactgg ggagcattac aaagtgaaac agatttaatt gcctttttag atgctgatga
    18541 tgcttatgaa aacggggcat tgcaagttgc tgaaaagatt tttgagttta aaccggaaac
    18601 cggcttggtt cggttagcat taaaaccggt aacttagatc aacgttacag ctctcaccct
    18661 aattttgagt tagcttggca atatatgcaa atgactggtg caggcaacac tgtatttcgg
    18721 cgtagtttct tcctcgcttg tggcggcttt ccacaacatc agctcttcca agaattaggt
    18781 ggagaagatg gtgctttagg catagccact acacaaatta gctctgtagc tactgcattt
    18841 aatgatgtcg gtgttttaca ttactgccga gagggtatgc acgcagaaag attattaaat
    18901 gcgattttat tcaacgaaaa agatcccaat gttactgaag aaaaagttaa acaagccaac
    18961 ctaattaccg aaaatattgt taataatatt agaaatctac aagactgtct taatagtaaa
    19021 gggattggta ttaagcctta tacgcttgaa tggagctaaa aaagcatttg cagattttcc
    19081 ctaaaaaaa
//
LOCUS       DANLUQ010000035        18946 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.035, whole genome shotgun sequence.
ACCESSION   DANLUQ010000035 DANLUQ010000000
VERSION     DANLUQ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 18946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18946
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.035"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            83..2518
                     /gene="plsB"
                     /locus_tag="PXB27_000969"
     CDS             83..2518
                     /gene="plsB"
                     /locus_tag="PXB27_000969"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HDL5345778.1"
                     /translation="MSGLLNFYRAFLNLPLSLLVKSRSIPTHPVAELELNLEQPIVYV
                     LPYTSQTDLLILQKNCLALNLPDPLQENVIEGQTLLRFVFLDEGRRFFKSKGAKSETE
                     SIFYRYLDLHRANAELDVQLVPVSVLWGRAPGKEDARHLQVLTSFQRFLSMVWFGRDN
                     FVRFSPAVSLRYMVTEYGADEKIAQKIARVAKMHFAKLRYSAMGPRLPNRDAMFNKIL
                     NSEVIQAAIAEEAKKSSPEKARKEAEKIINEIAADVKHESLRVADRVLSWLWNKLYQG
                     INVQNADRVRKLALEGHEIVYVPCHRSHMDYLLLSYLLYHQGLVPPHIAAGINLNFWP
                     AGPIFRSWGAFFIRRTFKGNRLYSTIFREYLAELFYRGYSVEYFIEGGRSRTGRLLEP
                     KTGMMSMSLQALQRGLNRSLSIVPVYIGYEHVLEVDTYAKELRGAAKEKENAGLVLRV
                     LKKLKNLGQGYVNFGEPILVNNYLNQYFPEWKEPSEDGRPKWLNEAVDNISHQVMVNI
                     NKAAAVNAKNLVGSALLASRQRALTREQLIEQLGSYIQLFRNVPYSKDMTLPTESAEV
                     MLNHVIHLPRSGVLIEKDNFGELVRLERESAVLMTYYRNNVQHLFVLPSLVASMVLHH
                     EAVSKDVVIKSVNRIYPFLQAELFLHFKQEELKAHIEAIIAELSRQGVIKNESDMLMI
                     NRSHIRTLQLHSAGVREILQRYYISLSVLLEQPNISRGYLEKESSSIAQRLSVLHGIN
                     APEFFDKAIFSTFTSSLKAQGYFDGDDNLAIAKVKETESLLRSLISIEVQQTIQGAMA
                     KVAESVENPNIAG"
     gene            complement(2623..3186)
                     /locus_tag="PXB27_000970"
     CDS             complement(2623..3186)
                     /locus_tag="PXB27_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HDL5345779.1"
                     /translation="MSAPNSGTNPAPLGLCGFALTTFLLSLINNGTFSGENVGLVLAM
                     GFAFGGTSQMIAGMFEFAKGNTFGFTAFTSYGAFWWSFALFKVFFAANVSAEFVGWYL
                     VAWGTFTLMMFIGTLAKARALQAIFLSLTITFYLLAAGDFTHNTTLTNIGGNFGLLTA
                     FLAFYLAAADIINESFGKTVLPIGEPK"
     gene            complement(3384..4280)
                     /locus_tag="PXB27_000971"
     CDS             complement(3384..4280)
                     /locus_tag="PXB27_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5345780.1"
                     /translation="MDILDRLLAFAQISAGISVKCQLQGDWQLENPHQTGQAIAHIIS
                     YGQAYLQHNGKTHHLKQGDIVFFPRFADHTLRSQIQPLTSKNAVNPEQLGAFTLTQAG
                     LSQSECDMFCLHFYYDHQAELMESLPEMIVFQVAETPLKAMLDILKQEAETPNLAVKS
                     VVNSLAVVILTMILREHLVNHNNQLFGILKGWQDLHLRPLIKAILLNPEHEWQVDDMA
                     AFIHLSRAQLVRLFNKHLCISPHAFVHKIRLQKAAMLLKSQAGSVLSIALSCGFQSES
                     HFITAFKKQYCVTPSQYRQLNP"
     gene            4416..4757
                     /locus_tag="PXB27_000972"
     CDS             4416..4757
                     /locus_tag="PXB27_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDL5345781.1"
                     /translation="MSSFQNWTEHTAHVKQSFGKLGQKHPKMLEAYKALGTAAAAEAL
                     DHKTRELIALAVAITTRCESCISVHAEEAAKAGATESEIAGALATAIALNAGAAYTYA
                     LRALEAVEEQK"
     gene            4768..5121
                     /locus_tag="PXB27_000973"
     CDS             4768..5121
                     /locus_tag="PXB27_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL5345782.1"
                     /translation="MKKLAWILGFAAMMPMMSSAKQASVQQVQAEKAAGIWIDVRSAE
                     EFQQGHLNGAINVTHTEIAQRIAEVAPDKNQPIHLYCRSGHRAEAALNELKKLGYSNV
                     TNHGGYDDLIKAGLK"
     gene            5181..5819
                     /locus_tag="PXB27_000974"
     CDS             5181..5819
                     /locus_tag="PXB27_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005719747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgB family protein"
                     /protein_id="HDL5345783.1"
                     /translation="MSAFNNFVNFVANIVAPMQRQFINFVRIAICIVMVWIGGLKVCQ
                     YEADGIAHFVSNSPFFSYMYKKGPNLVDDGTGKMVMEYTLHKNPEGKMVAKNIEWHKE
                     NGTYTASYIIGATIVTVGLLTLSGIWFPVSGMAGGLLTFGMSIVTLSFMITTPEIWVP
                     NLGGDMPTPAHGFPYLSAVGRLIVKDVIMMAGGLVAAAECANRILARRKAIA"
     gene            5954..7135
                     /gene="purT"
                     /locus_tag="PXB27_000975"
     CDS             5954..7135
                     /gene="purT"
                     /locus_tag="PXB27_000975"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005615456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HDL5345784.1"
                     /translation="MTTIGTPLRTNATKVMMLGSGELGKEVVIELQRLGVEVIAVDRY
                     DNAPAQQVAHRAYTISMLDGNALRDLVEKEKPDFIVPEVEAIATATLVELEQEGYNVI
                     PTAKATQLTMNREGIRRLAAEELGLKTSPYRFVDNFEQFQQAIQEIGIPCVVKPIMSS
                     SGHGQSVIKSEADIQQAWDYSQQGGRAGGGRVIVEGFIKFDYEITQLTVRHIHGTSFL
                     APIGHIQVDGDYRESWQPQQMSDIALKKAQETAEKITSALGGRGIFGVELFVCGDEII
                     FNEVSPRPHDTGIVTMASQELSQFALHARAILGLPIPEIYRISPAASKAIVVEGKSDN
                     VRFGGVDKVLAEIGTNIRLFGKGEVNGHRRLGVILARDENTVRALEKARRAYDKLDIQ
                     L"
     gene            complement(7227..8720)
                     /locus_tag="PXB27_000976"
     CDS             complement(7227..8720)
                     /locus_tag="PXB27_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005556599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL5345785.1"
                     /translation="MFKQLQQVGKAFMLPIAILPAAGLLLGIGGALSNKATVQAYPIL
                     DNPTLQGIFQIMASAGSVVFGNLALLLCIGLGIGLAKRDKGVAALAAVVGYLIMTGTI
                     SALIPLFPPETKGIDTGVIGSLVMGLITVKLHNKYHNIQLPQILGFFGGSRFVPIVTA
                     FAAIFVGAVFFLIWPAFQQWLVSAGQGIAEMGEIGTFFYGFLMRLSGAVGLHHMIYPL
                     FWYTELGGTEVVNGETIIGAQKIFFAQLADPTHQGLFTEGTRFFAGRFDTMMFGLPAA
                     CLAMYHAVPKARRGAISGLFLGAALTSFITGITEPIEFMFLFVAPWLYVFHAFLDGVS
                     FYIADLLNISIGNTFSGGVIDFTLFGILQGNENTNWLRVIIVGLPWAALYYFSFLFLI
                     KKFNVMTPGRAEESEEVVEQQTSSLTENAHKIIAALGSANNIENVDACITRLRVAVKE
                     VKAVDKATLKKLGAIDVLEVGGGVQAVFGAKAVLYKSEVNQILGKED"
     gene            8921..10768
                     /gene="ilvD"
                     /locus_tag="PXB27_000977"
     CDS             8921..10768
                     /gene="ilvD"
                     /locus_tag="PXB27_000977"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012055207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HDL5345786.1"
                     /translation="MPILRSATSTQGRNMAGARALWRATGMKENDFGKPIIAVVNSFT
                     QFVPGHVHLKDMGQLVAAEIEKAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRDLIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMAAMRLNIPTIFVSGGPMEAGKTKLSDQLI
                     RLDLVDAMIESADPNVSDERIDAIERSACPTCGSCSGMFTANSMNCLTEALGLSLPGN
                     GSMLATHADRKELFLRAGREIVELCRKYYQQDDESVLPRSIATFDAFENAMSLDIAMG
                     GSSNTVLHLLATAQEAGVDFKMEDIDRLSRVVPCLSKIAPNTNKYHMEDVHRAGGIMG
                     LLGELDRAGLIHRHVKTVLGLTIEEQLNQYDIIRNKDEDLHKFFRAGPAGIRTTKAFS
                     QDCRWDTVDDDRVNGCIRNVENAVTKEGGLAVLFGNIAEDGCIVKTAGVDESIWKFTG
                     TAIVFESQEDAVAGILGGKVKEGHIVIIRYEGPKGGPGMQEMLYPTSYLKSMGLGKKC
                     ALLTDGRFSGGTSGLSIGHASPEAASGGAIALINDGDTVAIDIPNRSINLMVSDEELA
                     ARRAEMEARGDKAWQPLKREREVSFALKVFGHFATSADKGAVRDKSLLK"
     gene            complement(10902..11252)
                     /locus_tag="PXB27_000978"
     CDS             complement(10902..11252)
                     /locus_tag="PXB27_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HDL5345787.1"
                     /translation="MWHFYLMTIELNNIQYPTDASGYLTNLNDWSVELAEKIAEKEQI
                     SLTAEHWEIIYLVRDFYQEYKTSPAIRMLVKTMAQKFGEEKGNSRYLQRLFPDGPAKQ
                     ATKIAGLPKPAKCL"
     gene            complement(11281..11946)
                     /locus_tag="PXB27_000979"
     CDS             complement(11281..11946)
                     /locus_tag="PXB27_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BAX inhibitor protein"
                     /protein_id="HDL5345788.1"
                     /translation="MENRLAHSYSTGESLISTHKVLRNTYMLLAMTLTFSAVVAFVAI
                     QMNAPAMPWWGLLVGFYGLLFLTNATSNSSAGILSVFALTGFLGYTLGPILNRYIGAG
                     LGDIVALAFGATALVFFACSAYVLTTKKDMSFISGMMVALFVVLLVGIIANIFLAIPA
                     LSLALSSLFVIFSSGAILLATSNIIHGGETNYIRATVDLYVSIYNLFVSFLQIFGVLG
                     SDD"
     gene            12152..12700
                     /gene="pgsA"
                     /locus_tag="PXB27_000980"
     CDS             12152..12700
                     /gene="pgsA"
                     /locus_tag="PXB27_000980"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDL5345789.1"
                     /translation="MKLNFPTYLTLFRVGLTPLFIVAFYLPTKYAPEISTLIFFIASI
                     TDAFDGYLARKWNQTTRLGAFLDPVADKVLVAVAFVCVAEYYHTWWITIPICIMIARE
                     IIISALREWMAELGERASVAVSIWGKVKTTAQMLALGGMLWRQNQLMEILAFILLYIA
                     VGLTIWSMMQYLNASKNSLLKS"
     gene            12778..14616
                     /gene="uvrC"
                     /locus_tag="PXB27_000981"
     CDS             12778..14616
                     /gene="uvrC"
                     /locus_tag="PXB27_000981"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDL5345790.1"
                     /translation="MSEFNAKSFLLNVPHQPGVYRMYDSKGTIIYVGKAKDLKKRLAS
                     YFRSQLSSKKTEALVANLANIETTITHSETEALLLEHNYIKENQPKYNVLLRDDKSYP
                     YILLTKHKHPRLTAFRGSKKIAGEYFGPYPNVTAVRETLNLLQKIFPIRQCEDTYYKN
                     RSRPCLQYQIGRCLAPCVEGCYSPVEYDQQVNLVRLFLQGKDHQVVEHLVQKMEDAAE
                     ALDFEGAARFRDQIQSVRAVQEKQFVSNDRLDDLDIISIVYQNGIACVHILFVRQGKV
                     LGNRSYFPKVPSNTDLTELADTFVGQFYLQMNQHRTIPNQIVIDHQLSESKALSEILS
                     EQAGHKVSILDNAIRGDKQRYLALARTNAMAALVLQLKQESHIQSRYQALKEMLKMSE
                     IKRMECFDISHTMGNQTVASCVVFDENGPLKSDYRRFNIEGITGGDDYAAMEQALLKR
                     YNRELPPEKIPDIIFIDGGKGQLNRALDVFAKLDVEWDKSKPLLIGVAKGVERKAGLE
                     TLIISKWDKEIHLPPDSLALHLIQHIRDESHQHAISGHRKKRQKAFTESGLESIAGVG
                     AKRRQALLKYLGGMQGVKSATLEEIQSVPGISKALAEVIFDTLKNS"
     gene            complement(14621..14896)
                     /locus_tag="PXB27_000982"
     CDS             complement(14621..14896)
                     /locus_tag="PXB27_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345791.1"
                     /translation="MKKLILLGLSVALTACSTPQQQSTVPMDMQAVQDYHQRVINKQT
                     VDKNTKQHDEPLNQSDKRSESINLPIHPIIYPSIGVGYHYGWKRYRY"
     gene            complement(15021..15374)
                     /gene="rplT"
                     /locus_tag="PXB27_000983"
     CDS             complement(15021..15374)
                     /gene="rplT"
                     /locus_tag="PXB27_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009635878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDL5345792.1"
                     /translation="MARVKRGVIARARHKKVLKAAKGYYGARSRVYRVAFQAVVKAAQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGLSYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFAALVEKAKSAL"
     gene            complement(15483..15680)
                     /gene="rpmI"
                     /locus_tag="PXB27_000984"
     CDS             complement(15483..15680)
                     /gene="rpmI"
                     /locus_tag="PXB27_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDL5345793.1"
                     /translation="MPKIKTVRGAAKRFKKTASGGFKRKQSHLRHILTKKTTKRKRQL
                     RHKSMVAKADQVLVVACLPYA"
     gene            complement(15944..16486)
                     /gene="infC"
                     /locus_tag="PXB27_000985"
     CDS             complement(15944..16486)
                     /gene="infC"
                     /locus_tag="PXB27_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015871208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDL5345794.1"
                     /translation="MKPVKRVQSNRAHRLNDEIRGVKEVRLTDENGEQVGIVSLQDAL
                     NRAEEAELDLVEISPNAEPPVCRIMNYGKFIYEKEKAAKEQKKKQKVVQVKEIKFRPG
                     TDDGDYQVKLRSIIRFLEDGDKVKITVRFRGREMAHQEIGMDVLERVKADTAEIAMVE
                     SAPGKLEGRQVVMVIAPKKK"
     gene            complement(16645..18444)
                     /gene="recQ"
                     /locus_tag="PXB27_000986"
     CDS             complement(16645..18444)
                     /gene="recQ"
                     /locus_tag="PXB27_000986"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HDL5345795.1"
                     /translation="MNTTAISVLNNIFGYQKFRDGQEEVINAVLENRDCLVIMTTGGG
                     KSLCYQVPALCLDGITLVISPLISLMKDQVDQLITNGIEAAYLNSTQTLEEQQLVEQK
                     ALSGQLKLLYLSPEKVMTQGFFHFISLCKISFIAVDEAHCVSQWGHDFRPEYTLLGGL
                     RNTFPNVPLMALTATADLTTRSDILHHLRLNSPHIYLGSFDRPNIRYTVQEKFKPLEQ
                     LIKFISKQQGKSGIVYCNSRKKVEEITEKLSARKISVMGYHAGMSFEQRERVQNAFQR
                     DDIQIVVATIAFGMGINKSNVRFVVHFDLPRSIESYYQETGRAGRDDLPSEAVLFYDP
                     SDYAWLQKILLEEPESEQRNIKQHKLQAIGDFAESQTCRRLVLLNYFGEHRQEQCKNC
                     DICLDPPQQYDGLLDAQKVMSVIYRTGQIFGQHHIIAVLRGMNNLKIREHQHDQLSVY
                     GIGKEQSQHYWQNVVRQLIHLGLIIQNIANHSALQLTESARPFLRGEKQLQLALPRLG
                     ISATAFVQKQASHRYDKDLFARLRFLRKQIADLENIPPYIVFNDATLQEMAEFLPTSK
                     VEMLEINGVGERKFERFGQAFMSLIKEYQNNRK"
     gene            complement(18553..18858)
                     /gene="cyaY"
                     /locus_tag="PXB27_000987"
     CDS             complement(18553..18858)
                     /gene="cyaY"
                     /locus_tag="PXB27_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HDL5345796.1"
                     /translation="MNTAQFHQAVEQVWQQIEEKIYDEGLSVDTEIQGAVCTLTFDDE
                     SQVIINKQEAMLELWLASKLGGFHFKNVEGEWVTDEGRSFWQHLEEAFARHGESVSF"
BASE COUNT         5506 a         3607 c         4149 g         5684 t
ORIGIN      
        1 gaaattccat aaaaatttat ggtagaatgg aagaaattag aggttagacc tccaacaata
       61 acgattctaa aaggatattt atatgtctgg cttattaaat ttttatcgag ctttcctaaa
      121 cttaccactt tcacttttag taaaatctcg ctctattccg actcatccgg tggctgaact
      181 ggaactaaat cttgaacagc cgattgtcta tgttctacct tatacctcgc aaaccgattt
      241 attaatttta caaaaaaact gcttggcttt gaatttgccc gatccgttgc aagaaaatgt
      301 gattgaaggg caaacattgc ttcgttttgt atttttagat gaaggtcgcc gtttttttaa
      361 atctaaaggg gcgaaaagtg agacagagtc gattttttat cgttatttag atttacatcg
      421 tgctaatgct gaattagacg tccaattagt gccggtttcg gtattatggg ggcgggctcc
      481 gggcaaagaa gatgcccgcc atttacaggt gcttacctca ttccaacgtt ttttatccat
      541 ggtttggttt gggcgtgata attttgtacg tttctcccct gcagtatcgt tacgctatat
      601 ggtaactgaa tatggtgcag atgaaaaaat cgctcaaaaa atcgcgcgtg tggcgaaaat
      661 gcactttgca aaattgcgtt attcagcaat ggggcctcgt ttaccaaatc gtgatgcgat
      721 gtttaataaa atcctgaatt cagaggtgat tcaagctgcg attgcggaag aagcgaagaa
      781 atcatcgcct gaaaaggctc gcaaagaagc cgagaaaatc attaatgaaa ttgccgctga
      841 tgtaaaacac gagagtttgc gtgtagcaga tcgagttctg agctggcttt ggaataagct
      901 ttatcaaggg atcaacgtgc aaaatgcaga ccgtgtgcgt aaattggcat tagaaggtca
      961 cgagatcgtt tatgtgcctt gccaccgtag ccatatggac tatttgctcc tatcttatct
     1021 actttaccat caaggattag taccgccaca tattgccgct gggatcaatt taaatttctg
     1081 gccggcaggc ccgattttcc gtagttgggg agcatttttc attcgccgta cctttaaagg
     1141 aaatcgcctc tattccacca ttttccgtga atatttagcg gaattattct atcgtggtta
     1201 ttcggtcgaa tattttattg aaggcgggcg ttcacgcaca ggtcgtttat tagagcctaa
     1261 aaccggtatg atgtcgatga gcctacaagc cttacagcga gggctgaata gatctttaag
     1321 tattgtgcct gtttatatcg gttatgaaca cgtcttagaa gtcgatactt acgctaaaga
     1381 gttgagaggt gcggcaaaag agaaagaaaa tgcgggcttg gtgctacgag tgctgaaaaa
     1441 actgaaaaat ttaggtcaag gatatgtgaa tttcggtgag ccaattttgg tgaataatta
     1501 cttaaatcaa tatttccctg agtggaaaga gccttccgaa gatggacgac ctaagtggtt
     1561 aaatgaagcc gtggataata tttcacatca agtaatggtg aatatcaata aagcggcagc
     1621 agttaatgcg aaaaacttag tggggtcagc attattggca tctcgccagc gtgcattaac
     1681 ccgtgaacaa ctcattgaac aacttggaag ttatattcag ttatttcgta atgtacctta
     1741 ttctaaagat atgacattac caactgaaag tgcagaagtg atgttgaatc atgttattca
     1801 tttacctcgt tcgggcgttt taattgaaaa agataatttt ggtgaactgg ttcgtttaga
     1861 acgagaatct gctgtgttaa tgacatatta cagaaataat gtgcagcatt tatttgtgtt
     1921 gccttcactt gttgcaagta tggtacttca tcatgaggct gtttcgaaag atgttgtgat
     1981 taagtcggtt aatcgtattt atccattctt gcaagcagag ttattcttac actttaagca
     2041 agaagaatta aaagcacata ttgaagcaat tattgcggaa ctaagtcggc agggggtgat
     2101 taaaaacgaa agtgatatgt taatgatcaa tcgctcacat attcgtacct tgcaattaca
     2161 ttcagctggt gtgcgtgaaa tccttcaacg ttattatatt agcctaagtg tcttgttgga
     2221 gcagccaaat attagtcgtg gctatttaga aaaagagagt agttcaattg ctcagcgttt
     2281 atcagtcttg catggtatta atgcgccgga gttttttgat aaggcgattt tctctacctt
     2341 tacatcaagt ctaaaagcac aagggtattt tgatggtgat gataatttag cgattgcaaa
     2401 agttaaagaa acggaatcgt tgttacgtag tttgatttcg attgaagttc aacagaccat
     2461 tcaaggtgcg atggctaaag tcgcagaatc tgtagagaat cctaatatcg caggataata
     2521 aatcaaagaa gggcgctaac aagcgctcgt ttcttgcaat tttgttgcca aaaataaaag
     2581 ctgaatgcgc gaccacattc agctttttta cttcctatat aattattttg gttcaccgat
     2641 tggtaaaaca gtcttaccaa aactttcatt aataatatcc gcagcggcta agtagaacgc
     2701 taagaatgcg gttaataaac cgaagttacc accaatgtta gttagggtgg tgttatgggt
     2761 aaaatcacct gcggctaaaa ggtagaaagt aatggttaaa cttaagaaaa tagcttgtaa
     2821 tgcacgggct tttgctagtg tgccgatgaa catcataagt gtgaatgttc cccaagctac
     2881 taagtaccaa ccaacgaatt ctgccgatac attcgcagca aagaacactt taaataatgc
     2941 aaaagaccac cagaatgcac catagctggt aaacgcagta aagccgaacg tgttaccttt
     3001 agcaaattca aacatacctg caatcatttg tgatgtgcca ccgaaagcaa atcccattgc
     3061 taaaactaaa cctacgtttt caccgctgaa tgtaccgtta ttgattaggc ttagtaaaaa
     3121 tgtagtaagt gcaaaaccgc ataaaccgag cggagccggg ttagttcctg aatttggagc
     3181 tgacattgaa aacctctgtt atattgttac accataagtg tggtaattgt ttatttacgc
     3241 cgataaatca gacgttatgt gcctaaaatt tagggcgata aagtatagcg attttgcagt
     3301 aaattgacta gaaatttccg agacaagcgg tgagattttt gcaaaatttt gccaaaaaat
     3361 gaccgcttgt tgttgtcatt tagctatgga tttaactgcc gatattggct gggtgttacg
     3421 caatattgct ttttaaaggc ggtaataaaa tgcgactcgg attggaagcc gcaagataag
     3481 gcgatactta ataccgaccc agcttggctt ttgagcagca ttgctgcttt ttgtagccta
     3541 attttgtgta caaaagcatg tgggctaata cataaatgtt tgttaaataa gcggactaac
     3601 tgggcacgag aaaggtggat aaatgccgcc atatcatcga cttgccattc gtgttcggga
     3661 ttaagcaaaa tagctttgat caaaggtctt aagtgtaagt cttgccaacc ttttaaaata
     3721 ccgaataact gattgttatg gttaactaga tgttctcgta agatcatagt taaaataaca
     3781 acagcaagcg agttaaccac agattttacg gctaaattag gtgtttctgc ttcttgtttt
     3841 agaatatcta acattgcttt taatggtgtc tcggcaactt gaaatacgat catttcaggt
     3901 aagctttcca ttaattcggc ttgatggtca taataaaaat ggaggcagaa catatcgcat
     3961 tctgattgag aaagtcctgc ttgggtgagt gtaaatgcac ccaattgttc agggtttacc
     4021 gcatttttgc tggtcaaagg ctgtatttga ctacgtaatg tatgatcggc aaatctgggg
     4081 aaaaatacaa tatctccctg tttaaggtga tgggttttcc cattatgttg tagataagct
     4141 tgcccatagc tgatgatatg agcgattgct tgtccggttt gatgcggatt ctcaagttgc
     4201 caatctccct gtaactgaca tttcacactg attccggctg agatttgagc gaaagctaga
     4261 agcctatcta atatatccat tgttcattcc ttatatttcc accttaaatt gcgattattt
     4321 cattataata tgagctgatt tgataataaa gtttatcaga taaacgcaat aataaatgcg
     4381 ttttttcata ttattcactt taggaggaaa tcaatatgtc ttcattccaa aattggacag
     4441 aacacacagc tcacgttaag caatcttttg gtaaattagg gcaaaaacac cctaaaatgc
     4501 ttgaagctta taaagcatta ggtacagcgg ccgcagcgga agcattagat cacaaaactc
     4561 gtgaattaat tgcattagca gtagcgatta caacacgttg tgaaagttgc atcagtgtac
     4621 acgcagaaga agcggcaaaa gcaggtgcaa ccgaaagcga aattgccggt gcattagcga
     4681 ctgcgattgc attaaatgcc ggtgcggcat atacctatgc tttacgtgca ttagaagcgg
     4741 tggaagagca aaaataagga gcaataaatg aagaaattgg cttggatttt aggctttgca
     4801 gctatgatgc caatgatgtc tagtgctaaa caagcatcag ttcagcaagt gcaggctgaa
     4861 aaagcagcgg gcatttggat tgatgtacgt tctgctgaag aatttcagca agggcattta
     4921 aatggtgcaa ttaatgtaac gcatactgaa attgcacaac gtatcgcaga agttgctcca
     4981 gataaaaatc agccgattca tctttattgc cgctcgggtc atcgagctga agcggcgctt
     5041 aatgagctaa aaaaattagg ttacagcaat gtaactaatc atggtggtta tgacgatctg
     5101 attaaagccg ggttgaaata actgttttga gtgccaagca tttgcttggc acttttcgtt
     5161 tatatctgag gttaaaaatt atgagtgctt ttaacaattt tgttaatttt gttgcaaata
     5221 ttgttgctcc aatgcaacgc caattcatta actttgtacg tattgccatt tgtatcgtga
     5281 tggtttggat tggtggttta aaagtctgcc aatatgaagc agacggtatt gcccattttg
     5341 tgtctaatag cccgttcttt agttatatgt acaaaaaagg cccgaattta gttgatgacg
     5401 gcacaggtaa aatggtgatg gaatataccc ttcacaaaaa ccctgaaggt aaaatggtgg
     5461 caaaaaacat cgaatggcat aaagaaaacg gcacttatac tgcatcttat atcatcggtg
     5521 caaccattgt gacggttggt ttattgacgt tatccggtat ttggttcccg gtttccggta
     5581 tggcgggggg cttactcacc tttggtatgt cgattgttac actctctttt atgatcacca
     5641 cccctgaaat ttgggtgccg aatttaggtg gggatatgcc aaccccagcg catggtttcc
     5701 catatctgtc agcagtcggt cgtttaattg tgaaagatgt gattatgatg gcaggcggct
     5761 tggttgccgc agcggaatgt gcaaatcgca ttttagccag acgtaaagcg attgcttaat
     5821 tgatagtggt aatgcaagcg gtcattttct tgtaaaattt tgcaataaaa tgaccgcttg
     5881 ttttttgtca atttgccata atttcaagta taattcaatt acgtaatcgt ttgcttttat
     5941 ggagaggaat aaaatgacaa caatcggtac accgttaaga acgaacgcta caaaagtgat
     6001 gatgttaggt tcgggcgagt tggggaaaga agtggtaata gagctgcaac gcttaggcgt
     6061 agaagtgatt gcggtggatc gttatgacaa tgcacctgca cagcaagtgg ctcaccgtgc
     6121 ttatacgatt tcgatgttag acgggaatgc attgcgagat ttggtggaga aagaaaaacc
     6181 ggattttatt gtgcccgaag tagaagccat cgcaactgca accttggttg agttagagca
     6241 agagggatat aacgtaatcc caacggcaaa agcaacgcaa ttaacaatga atcgagaagg
     6301 cattcgccga ttggcggcag aagagttggg cttaaaaacg tcgccttatc gttttgtgga
     6361 taattttgaa cagttccagc aagcgattca agaaatcggc attccgtgtg tggtcaaacc
     6421 gattatgtct tcttccggac acgggcaatc agtaattaaa tctgaagcgg atattcaaca
     6481 ggcttgggat tattcgcaac agggcggacg tgccggcggt gggcgtgtga ttgtagaggg
     6541 ttttatcaaa ttcgattatg aaattaccca gcttaccgtt cgccatattc acggcacgtc
     6601 gtttttagct cccatcgggc atattcaggt tgatggcgat tatcgtgaat cttggcaacc
     6661 gcagcaaatg tcggacattg cattgaaaaa agctcaagaa accgcagaaa aaattacctc
     6721 tgcactgggc ggaagaggga ttttcggcgt ggaacttttt gtctgtggcg atgagattat
     6781 ttttaacgaa gtctccccac gtccacacga taccggaata gtaacaatgg catcacaaga
     6841 gctttctcaa tttgctctgc acgcccgtgc gattttgggc ttaccgattc cggaaattta
     6901 ccgcatcagc ccggcagctt ctaaagcgat tgtggttgaa gggaaatcgg ataatgtgcg
     6961 ttttggcggt gtagataagg tattagccga aatcgggacg aatatccgct tattcggtaa
     7021 aggcgaagta aacggacacc gccgattagg ggtaattctt gctcgtgatg aaaatacagt
     7081 gagagcatta gaaaaagctc ggcgagctta tgataagtta gatattcaat tataaagtca
     7141 ggattttgat attcgttgat agtaggcatt ttatgcctac tatcctatag gcctctaatc
     7201 aaattggatg aaaaagttga agattgttaa tcctctttgc cgagaatttg gttcacttcg
     7261 ctcttataca atactgcttt tgctccaaat acggcttgta caccgccacc gacttccagc
     7321 acgtcaatcg caccgagttt tttcagtgtt gctttatcca cggctttcac ctctttcact
     7381 gccacacgca gacgggtaat acaagcatcc acattttcga tattatttgc agagccaaga
     7441 gcggcgataa ttttatgggc attttcagtc agtgagctgg tttgttgttc gactacctct
     7501 tcactctctt ccgcacgtcc cggtgtcatt acgttgaatt ttttgatcag gaataggaac
     7561 gagaagtagt aaagtgccgc ccacggcaag ccgactatga tcactcgaag ccagttagtg
     7621 ttctcgttgc cttgcaaaat gccaaacagg gtgaagtcga tcacgccacc ggagaaggtg
     7681 ttaccaatcg agatgttgag taagtctgca atgtagaacg acacgccgtc taaaaaagcg
     7741 tggaacacat agagccacgg ggcaacaaac aggaacataa attcaatcgg ctcggtaatg
     7801 ccggtgataa aagaggtgag cgctgcacct aagaacagac cgctaatcgc accacggcgg
     7861 gctttcggca cagcgtgata catcgctaaa caggcggcag gtaagccaaa catcatcgta
     7921 tcgaaacgtc cagcgaagaa acgtgtacct tcggtaaata agccttgatg ggttggatcg
     7981 gcgagttggg cgaagaagat tttttgcgcc ccgataatgg tttcaccatt taccacttct
     8041 gttccgccca attcggtgta ccaaaacagc gggtagatca tatggtgtaa gccgaccgca
     8101 ccgcttaacc gcattaagaa accgtagaaa aaggtgccga tttcgcccat ttccgcaatc
     8161 ccttgccctg ctgaaaccaa ccactgttgg aaagccggcc agatcaaaaa gaacactgcc
     8221 ccgacaaaaa tcgcagcaaa ggcggtcaca atcggcacaa accgagagcc gccaaagaag
     8281 ccgagaattt gcggcagctg aatgttgtgg tatttgttat gcagtttcac cgtaatcaag
     8341 cccattacca gcgagccgat cacaccggta tcaatccctt tggtttcagg aggaaacagc
     8401 ggaatgaggg cagaaatcgt gcctgtcata atcaaataac cgaccactgc tgccagtgcc
     8461 gctacgcctt tatctcgttt tgctaatcca atgcctaaac cgatacacag taataatgca
     8521 aggttgccaa acaccacact gcctgccgat gccataatct ggaaaatgcc ctgtaaagtc
     8581 gggttatcca gtatcgggta ggcttgcacc gttgccttat tcgacaaagc tccgccaatc
     8641 cctaacagca aaccggctgc cggtaaaatc gcaatcggca gcataaaagc ttttccgact
     8701 tgttgcagtt gtttgaacat aaaatttctc cttttttatg gttgtatttg atggtttata
     8761 gcatactgaa atttagccgt atgaggtgtg atataactca cagatttgca aaatttttag
     8821 caaattcgac cgcttgtaaa aaaattaccg cttgcggaaa aattgaagta gaattaagca
     8881 tttttttagc aaacacaaca taaaaaattt ggagaagatt atgcctattt tacgttcagc
     8941 aacttccact cagggtcgta atatggcggg ggctcgtgca ttatggcgtg caaccggtat
     9001 gaaagaaaat gattttggta agccgattat tgcagtggtg aactcattca cccaatttgt
     9061 gccggggcac gtccacttaa aagatatggg gcagttggtt gcggcagaaa ttgaaaaagc
     9121 cggtggggtg gcaaaagaat tcaacaccat tgcggttgat gacggcattg caatgggaca
     9181 cggcggaatg ctttattctt taccaagccg tgatttaatt gcagacagtg tggaatatat
     9241 ggtgaatgct cactgtgctg atgcgatggt gtgtatctct aactgcgata aaatcacccc
     9301 gggaatgtta atggcagcaa tgcggttaaa tattccgact atttttgtct ctggtggccc
     9361 gatggaagcc ggaaaaacta aactttccga tcaattaatt cgcttggatt tagtcgatgc
     9421 aatgatcgaa agtgccgacc cgaatgtgtc tgatgaacgt attgatgcga ttgaacgtag
     9481 tgcttgcccg acttgtggtt cttgttcagg tatgttcacc gcaaactcaa tgaactgttt
     9541 aactgaagct ttaggcttaa gtttaccggg caatggttca atgttggcaa ctcatgctga
     9601 ccgtaaagag cttttcctaa gagcaggtcg tgaaattgtg gaactttgcc gtaaatatta
     9661 ccaacaagac gatgagagcg tgttacctcg ctcaatcgct accttcgatg catttgaaaa
     9721 tgcaatgagc cttgatattg caatgggtgg ttcaagtaat accgtgcttc atttactagc
     9781 gactgctcaa gaagccggcg ttgattttaa aatggaagat attgaccgcc tctctcgtgt
     9841 cgtgccgtgc ttaagtaaaa ttgccccgaa taccaataaa taccatatgg aagacgtaca
     9901 ccgtgccggt gggattatgg gcttgctggg cgaattagat cgtgcaggtt taatccatcg
     9961 ccacgttaaa acagtgctag gtttaacgat agaagagcaa ctgaatcaat acgatattat
    10021 tcgtaataaa gatgaagatt tacacaaatt cttccgtgca ggtccggcag gaattcgtac
    10081 tactaaggct ttctcgcaag attgccgttg ggacactgtt gatgatgacc gtgtaaacgg
    10141 ctgtattcgc aatgtggaaa atgctgtgac caaagagggc gggttagcgg tattatttgg
    10201 gaatattgca gaagatggat gtattgttaa aaccgcaggg gtagatgagt ctatttggaa
    10261 attcactggc accgcaatcg tgtttgaaag ccaagaagat gcggtagcag gtattttagg
    10321 cggaaaagtg aaagaagggc atattgtgat tatccgctat gaaggtccga aaggcggccc
    10381 gggtatgcaa gaaatgcttt atccaactag ttatttgaaa tcaatgggct taggcaaaaa
    10441 atgtgcttta ttaactgacg gtcgtttctc tggcggtacc tcagggttat ctatcggaca
    10501 cgcttcaccg gaagccgctt caggaggagc gattgcctta attaatgatg gcgatacggt
    10561 ggcgattgat attccgaatc gttcgattaa cttaatggtt tcagatgaag aacttgctgc
    10621 tcgccgtgca gaaatggaag cccgtggcga taaagcgtgg cagccgctaa aacgtgagcg
    10681 agaagtgtcg tttgcactaa aagtgtttgg acattttgcc acctctgctg ataaaggtgc
    10741 ggtgagggat aaatcgctgt taaagtaaat aaattagaaa aaagaagcat ttcaataata
    10801 tattgatctg ctctttattc agctgaatat ttaatctagc tgaatggaga gcagatcgat
    10861 agcctaattt acatcttttt gttttgatac agggaaatgt attacaagca ttttgccggt
    10921 tttggcagac ctgcaatttt agtggcttgt tttgcaggac catcaggaaa caagcgttgt
    10981 aaataacggc tgttgccttt ttcttcaccg aatttttgtg ccattgtctt taccagcatt
    11041 cgaattgctg gtgaagtttt gtattcttgg tagaaatccc gcaccagata aataatttcc
    11101 caatgttctg cggttaagct aatttgctct ttttcggcga ttttttctgc taattctaca
    11161 gaccaatcat ttaaattagt taaatagcct gaagcatcgg ttgggtattg gatattattt
    11221 aattcaatcg tcatcaaata aaaatgccac ataatgtggc attcctctta aactaaaacg
    11281 ctagtcgtca ctacctagca caccgaagat ttgtaagaag cttacaaata ggttgtagat
    11341 agacacatat aaatcaacgg ttgcacgaat gtagttggtt tcgccaccgt gaataatgtt
    11401 gctggttgcc agtaaaatcg caccgcttga gaaaatgaca aacaagctgc ttagggctaa
    11461 acttaatgcc ggaattgcta agaagatatt ggcaataata ccgactaata ataccacaaa
    11521 cagggcaacc atcattccgc tgataaagct catatctttt ttggtggtta atacataagc
    11581 tgaacaagcg aagaaaacca atgcggttgc accgaatgca agggcgacaa tatcccctaa
    11641 acccgcaccg atgtaacggt ttaaaatagg acctaaggta tagcctaaga agccggttaa
    11701 agcaaatacg cttaagatac ccgcactgct gtttgaggtg gcattcgtca agaaaagtaa
    11761 gccgtaaaag ccgaccaata atccccacca cggcatagcc ggtgcgttca tttgaattgc
    11821 aacaaaagca acaactgcgg agaaagtcag ggtcattgcc aataacatat aggtattgcg
    11881 aagcactttg tgggtgctaa ttagagattc accagttgaa tagctgtgag ctaaacgatt
    11941 ttccataaaa attccttctg tttttgtgtt taaatcggaa catccgatgg attaatatct
    12001 atggataaat ttccataaat caagaagtaa ggtagccgta atttattgta aagtcaatgc
    12061 gattttgtaa cagaagagta aaaaaaaaca tttagttgtg ttacacttaa taccactcta
    12121 ctttatattc cttttcatcg atgaattcgc tatgaagctc aatttcccaa cttatttaac
    12181 cttatttcgg gtaggattaa ccccactttt tattgtggct ttttatctgc cgacaaaata
    12241 tgcgcctgaa atttcaacac ttattttttt tattgcatcg attaccgatg cctttgacgg
    12301 ctatttagca cgcaaatgga atcaaaccac ccgacttggt gcatttttag atcctgttgc
    12361 ggataaagtc ttggtagctg ttgcgtttgt ctgcgtggcg gagtattacc atacttggtg
    12421 gattactatt ccaatctgca ttatgattgc ccgtgaaatt attatttcgg ctttgcgtga
    12481 atggatggcg gagcttgggg aaagggccag tgttgccgtt tcgatttggg ggaaagtaaa
    12541 aacgactgct caaatgctcg cattaggtgg aatgttatgg agacagaacc aactaatgga
    12601 gattttagcc tttattctgc tttatattgc agtgggttta acgatctggt ctatgatgca
    12661 gtatttaaat gcttcaaaga atagtctatt gaagtcataa gcggtcatat ttttattctt
    12721 ttttgcaatt tgtagagtgg aaatatcgct ctacaatttt caattttatc aactctaatg
    12781 tctgaattta acgctaaatc cttcctgtta aatgtgcctc accaaccggg tgtttatcgt
    12841 atgtatgata gtaaaggcac gataatctat gtgggtaaag ccaaagattt aaaaaagcgt
    12901 ttagccagct attttcgtag ccaactttcc agtaaaaaaa cggaagcctt ggtggcaaat
    12961 cttgctaata ttgaaacgac gatcactcat tctgaaacag aggcgttact ccttgaacat
    13021 aattatatta aggaaaatca gccgaaatat aatgtgttgc tcagagatga caaatcttat
    13081 ccttatattc tgttaactaa acataagcat cctcgtttaa cagcatttcg tggtagtaaa
    13141 aagatcgccg gtgaatattt tgggccttat ccgaatgtta cggcagttcg ggaaacactg
    13201 aatttattgc aaaaaatttt tcctattcgt cagtgtgaag atacttatta taaaaaccgt
    13261 tcacgacctt gcttacagta tcagatcgga cgctgtttag ccccttgtgt agagggctgt
    13321 tattcacccg ttgaatacga tcagcaggtt aatttggttc gtttgttttt acagggaaaa
    13381 gatcaccaag tagtcgagca tctggtacag aaaatggaag atgcggcgga ggctttggat
    13441 tttgaaggtg cggcacgttt tcgggatcaa attcaatcag taagagcggt gcaagaaaaa
    13501 caattcgtct ctaatgatag gctagatgat cttgatatta tctcgatcgt ttatcaaaat
    13561 gggattgcct gtgtgcatat cttatttgtt aggcaaggta aggtactagg caatcgttct
    13621 tactttccaa aagtgccaag taatacagat ttaaccgagc tcgcagatac ctttgtcggt
    13681 cagttttatt tacaaatgaa ccagcaccgc accataccta accaaattgt aattgatcat
    13741 caattaagtg aatctaaggc actcagtgaa attttaagtg agcaagcagg gcataaagtc
    13801 agcattttag ataatgccat cagaggtgat aaacagcgtt atttagcttt agcgagaact
    13861 aatgcaatgg cagcattagt tttacaactt aagcaagaat cccatattca aagccgttat
    13921 caggcattga aagaaatgtt gaaaatgtca gagattaaaa gaatggagtg ctttgacatc
    13981 agtcatacaa tgggcaacca aacagtggct tcttgcgtgg tgtttgatga aaatgggccg
    14041 ctaaaatcgg attaccgccg ttttaacatt gaggggatta ccggaggtga tgattatgca
    14101 gcaatggagc aggctctctt gaaacgctat aatcgagaat taccgccaga aaagatccct
    14161 gatattattt ttattgatgg tggcaaaggg cagttgaacc gagcgttaga tgtcttcgct
    14221 aaacttgatg tggaatggga taaatcgaaa ccgcttttaa ttggtgtggc aaaaggcgta
    14281 gaacgtaaag ctgggctgga aactttaatt atcagcaaat gggataaaga aattcactta
    14341 ccgccagata gtcttgcctt gcatttaatt cagcatattc gagatgaatc ccaccagcat
    14401 gccattagtg ggcatcgtaa aaaacgccaa aaggccttta ccgaaagtgg attagaatct
    14461 attgccggtg tcggtgcaaa acgccgacaa gcgttgctaa aatatttagg cggaatgcag
    14521 ggggtgaagt ctgcaacctt ggaagagatt caatcagttc cgggaatttc taaagcgttg
    14581 gcagaggtta tttttgatac attaaaaaat agttaaatta tcaatagcga tatcgcttcc
    14641 agccgtaatg gtagcccact cctatgcttg ggtaaattat agggtgaatt ggcagattta
    14701 tgctttctga gcgtttatcg ctttggttta atggctcatc atgttgcttt gtatttttat
    14761 caacagtttg cttattgatt actcgttgat ggtaatcctg aactgcttgc atatccattg
    14821 gtactgtgct ttgttgttgg ggtgtcgaac aggcggttag tgcaacgctc aatccgagta
    14881 aaattaattt tttcatatca tttccttata ttgcaaaata ttttgctttt ttgaccgctt
    14941 attttgctat taattcttaa aaagattatt ttatccataa aaaaggacgc actaggcgtc
    15001 ctcatttcta ttctttaaaa ttaaagagca gattttgctt tttcaactaa agctgcgaaa
    15061 gcaactttgt caaatactgc gatgtcagct aagatcttac ggtcgatttc aacagatgct
    15121 tttttcaagc cgttgatgaa tttgctgtaa gataaaccgt tttgacgagc tgctgcgtta
    15181 atacgagcaa tccataattg acggaattga cgtttacgtt gacggcggtc acggtaagca
    15241 tattgagcag ctttaactac tgcttggaaa gcaacgcgat acacgcgtga acgtgcacca
    15301 taataacctt tagcagcctt aagaactttc ttatggcgtg ctcttgcaat aacaccacgt
    15361 tttacacgag ccattattta atctcctaat tgtctatttt gattggtggt cgtcatcacg
    15421 acctttacta agaattctat ccgtacgtcc gtactttact acactacgct aacctaaaag
    15481 gcttatgcgt atggtaagca cgctactact aaaacttggt cagctttcgc taccattgat
    15541 ttgtgacgta attgacgttt acgtttagtg gttttcttag tcaaaatgtg acgtaagtga
    15601 gattgtttac gtttaaaacc gccggaagct gtttttttga aacgcttagc agcaccacgt
    15661 actgttttaa ttttaggcat tgttaaaaaa ctccgcattg tttgttagat attatgaaaa
    15721 taaggcgaat tttgtgaaca aaattacttg gaagcactta ttctccgcta tacgattttc
    15781 atcgttaaaa agtcaagcag gctgctgagt ttgcctgttg agcattttct ctttcttggc
    15841 taggcaaaaa agcttgcgaa ttttagcgaa aatataaccg ctatgcaagc caatttaagc
    15901 tttatcaaga aaaaagcaca ttaaaacaat gtgcttatcg aaattatttc ttcttaggtg
    15961 cgatcaccat cacaacttgg cgaccttcca atttgcctgg ggctgactct accattgcaa
    16021 tttctgcagt atctgctttg acacgttcta aaacgtccat accgatttct tggtgagcca
    16081 tttcacgacc acggaaacgc actgtgattt ttactttatc cccatcttct aagaaacgga
    16141 taatgctacg aagtttaact tgataatcgc catcatcagt tcccggacgg aatttgatct
    16201 ctttaacttg aacaactttt tgttttttct tttgttcttt tgctgctttc tctttttcgt
    16261 agatgaactt accatagttc ataatacgac aaactggtgg ttcagcattt gggctgattt
    16321 ccactaaatc caattcagct tcttctgctc tatttagagc atcttgaagt gatacaatac
    16381 caacttgttc gccgttttca tcggttaagc gaacttcttt tacgccacga atttcgtcat
    16441 taagacgatg tgcacgattt gattgaacac gttttacagg tttaatagcc ttattccttc
    16501 tagtaaattt ttgcttaaat tttaccgctt gagagcgtat aaatatttta gattgcttgt
    16561 tttaagtgat taaaaaacag ctaaacgtgc ggattctata agattttcag tgcttatgca
    16621 ataataaagc ccttatttaa ctgcctattt tcggttattt tgatattctt tgattaaact
    16681 cataaatgct tgcccaaatc gttcaaattt tcgttcacca acaccattaa tttctaacat
    16741 ttccactttt gaagtcggga gaaattctgc catttcttgc aaggtggcat cgttaaagac
    16801 gatataaggt ggaatatttt cgaggtcagc tatctgcttg cgtaaaaaac gtaagcgtgc
    16861 gaataggtct ttgtcatagc gatgacttgc ttgtttttgc acaaatgcag tcgcagatat
    16921 acctaaacgt ggcaatgcaa gctgaagttg cttttctcca cgcaaaaatg gtctggcact
    16981 ttcggttaat tgcagagcgg aatggttagc aatattttga ataatcaacc ctaaatgaat
    17041 cagttgccgc accacatttt gccaataatg ttggctttgt tcttttccga taccataaac
    17101 ggaaagttga tcgtgttgat gttcacgaat tttgagattg ttcattcctc gcaataccgc
    17161 aataatatgg tgctgaccga aaatttgacc ggtgcggtaa atcaccgaca tcactttttg
    17221 ggcatcaagt aagccatcgt attgttgcgg tggatctaaa caaatatcgc agtttttgca
    17281 ttgctcttgg cgatgttccc caaagtagtt gagtaagact aaacggcggc aagtctgtga
    17341 ttcggcgaaa tcgccaattg cctgtaattt atgctgttta atatttctct gttcgctttc
    17401 cggctcttcc aataatattt tttgtaacca agcataatcg ctaggatcat aaaaaagcac
    17461 ggcttcagaa gggagatcat ctcgcccggc tcgtccggtt tcttgatagt aagattcgat
    17521 agagcgaggt aaatcgaaat gtaccacaaa acgtacgttg gatttgttga tccccatacc
    17581 aaacgcaata gtggcaacca caatttgaat atcatcacgc tgaaaggcat tctgcactcg
    17641 ttctctctgt tcaaaactca taccggcgtg atagcccatc acgctgattt tacgggctga
    17701 gagtttctcg gtgatttctt ccactttttt gcgactgttg cagtaaacaa taccgctttt
    17761 gccttgctgt ttgctgatga atttaatgag ctgttcaagc ggtttaaatt tttcctgcac
    17821 ggtgtagcga atattggggc gatcaaagct gcctaaatag atatgtgggc tatttaagcg
    17881 taaatgatgc agaatatcgg agcgggttgt tagatctgcc gtggcggtga gtgccatcaa
    17941 cggcacattc gggaaagtgt ttcgtaagcc acctagcagg gtatattcag ggcggaaatc
    18001 gtgcccccat tgggaaacac aatgggcttc atcaaccgca ataaagctga ttttacatag
    18061 cgaaataaag tggaaaaagc cttgagtcat taccttttca ggggaaagat agagtaattt
    18121 taattgaccg gataaggctt tttgttccac aagttgctgc tcttcgagcg tttgagtgga
    18181 gttaagataa gctgcttcaa tgccgttggt gataagttga tccacctggt ctttcatcaa
    18241 cgaaataagc ggcgaaatca ccaaagtgat gccgtctagg cacaaagccg gcacttggta
    18301 gcaaagggat ttgccaccgc ctgttgtcat aatcacgagg caatcacgat tttccaacac
    18361 ggcattgata acttcttctt gcccgtctcg gaatttttga tagccaaaaa tattatttaa
    18421 aacagaaatt gcggtggtgt tcattggtag ttggttgcta cttatgatag tactcgtctt
    18481 ctgagagtat cttttaaaat gaaaataaag cactcatcaa agacgagtgc tgtcttagtt
    18541 aacaattagg cattagaaag aaacgctttc tccgtgtcga gcaaaagctt cttctaaatg
    18601 ttgccagaac gagcgacctt catctgttac ccattcgcct tctacatttt taaagtggaa
    18661 accacccaat ttgctggcaa gccataattc cagcattgcc tcttgtttat taatgataac
    18721 ttgcgattca tcatcaaagg ttaaggtgca gaccgctcct tggatttcag tatcgactga
    18781 taatccttcg tcataaattt tttcttcaat ttgttgccat acttgctcta cggcttggtg
    18841 gaattgtgct gtgttcattg gtgtatcctt atttgaatgt tatataagta gtaaatactt
    18901 tttgttttag ctgtttaaat ggtctgtaaa gttctttttg tatttt
//
LOCUS       DANLUQ010000036        18832 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.036, whole genome shotgun sequence.
ACCESSION   DANLUQ010000036 DANLUQ010000000
VERSION     DANLUQ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 18832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18832
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.036"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            15..965
                     /gene="tal"
                     /locus_tag="PXB27_000988"
     CDS             15..965
                     /gene="tal"
                     /locus_tag="PXB27_000988"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDL5345797.1"
                     /translation="MSQLDTLSKMTVIVADTGDIEAIKLYKPKDATTNPSLILSASAL
                     PQYAPLIEEAINYAKAQSSDKDQQLVDAEDKLAVNIGLEILKLVPGRISTEVDARFSY
                     DTEKTIAKARKLIGLYNDAGISNDRILIKIASTWQGIKAAEQLEKEGINCNLTLLFSQ
                     AQARACAEAGVYLISPFVGRILDWYKAHEGKEYAPAEDPGVISVTNIYNYYKQHGYDT
                     IVMGASFRNVGEIIELAGCDRLTISPPLLKELQESDEKLVCKLDYKGEIKPRPEKMTE
                     AEFYWEHNQEPMAVEKLAEGIRKFAIDQEKLEAMLAEKLQ"
     gene            complement(1027..1300)
                     /locus_tag="PXB27_000989"
                     /pseudo
     CDS             complement(1027..1300)
                     /locus_tag="PXB27_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599987.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
     gene            complement(1331..1795)
                     /locus_tag="PXB27_000990"
     CDS             complement(1331..1795)
                     /locus_tag="PXB27_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDL5345798.1"
                     /translation="MKKLALAVALMSSFALVGCANTDVFSGSVYSGEQAKEARSIAYG
                     TIVSVRDVKIQARSEGVIGTVGGGVLGGVAGNAIGGGTGQAIATAVGAVAGAVIGNQV
                     EQKASQVSSLEMVIRKDDGKEIVVVQKKEDGFVPGKRVRIVGSNSDLNVSLL"
     gene            complement(1816..2415)
                     /locus_tag="PXB27_000991"
     CDS             complement(1816..2415)
                     /locus_tag="PXB27_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL5345799.1"
                     /translation="MDKIIAENAKFFEKMTACFGDLSQFEQKIRQIAEIAGIDLAAYE
                     IDHLAVRMNQTETAEQWKILLLDGATLLKESEVNGRPIGLFALIQAVEFCGQFVKIVE
                     LPFPKNKVYLEEGWEHIELVFPMLDNESSEQWVSRALSHFRLADNSEIKLKISQPQVE
                     GERLPNPSIAISLRNVTFCNPYCLKLHPYDIKSVIMSEN"
     gene            2449..4176
                     /gene="argS"
                     /locus_tag="PXB27_000992"
     CDS             2449..4176
                     /gene="argS"
                     /locus_tag="PXB27_000992"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDL5345800.1"
                     /translation="MNIQQILSDKIKQAMVASGADSNVEPLVRQSGKPEFGDYQANGA
                     MGAAKKLGMNPREFAQKILENADLNGIADKLEIAGPGFINIFLNPEWLAENTNSSLQA
                     VNFGIKTANPQTIVIDYSSPNVAKEMHVGHLRSTIIGDAVVRTLAFLGNNVIRANHVG
                     DWGTQFGMLIAYLEKMENEHASEMELSDLEAFYRAAKEHYDSDEAFAEKARNYVVKLQ
                     SGDKYCLTMWKKLVDITMHHNQENYNRLNVTLTEKDVMGESLYNPMLPEIVADLKAKG
                     LAVEDDGALVVFLDEFKNKDGDPMGVIVQKKDGGFLYTTTDIAAAKYRYETLKADRAL
                     VFSDSRQAQHMQQAWLITRKAGYVPDSFSLEHPFFGMMLGKDGKPFKTRSGGTVKLKD
                     LLDEAVERADKLIAERSTELTAEEKAAVVEAVAIGSVKYSDLSKNRTTDYVFDWDNML
                     TFEGNTAPYMQYAYTRIRSIFARANIDENALSGSIQLNEPKERALAVKLLQFEEALNG
                     VAKEGMPHILCQYLYELAGSFSSFYEACPILNAEETTKNSRLKLAALTAKTLKQGLDL
                     LGIKTVEKM"
     gene            4366..5532
                     /gene="cgtA"
                     /locus_tag="PXB27_000993"
     CDS             4366..5532
                     /gene="cgtA"
                     /locus_tag="PXB27_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HDL5345801.1"
                     /translation="MKFIDEALIRVEAGDGGNGCVSFRREKFIPKGGPDGGDGGDGGD
                     VYLIADENLNTLIDYRFEKRYAAGRGENGRSAGCTGHRGQDITLRVPVGTRAIDNDTQ
                     EIIGDLTRHGMKMLVAKGGYHGLGNTRFKSSVNRAPRQKTNGTPGEKRDLKLELLLLA
                     DVGMLGLPNAGKSTFIRSVSAAKPKVADYPFTTLVPSLGVARVGADRSFVVADIPGLI
                     EGAADGAGLGIRFLKHLERCRILIHLVDIMPIDESDPAHNISVIESELYQYSEKLAEK
                     PIWLVFNKIDTIGEEEAAERAKDIAEQIGWEGDYYLISAATGQNVQALTRDIMDFIEA
                     NPREVEEENKAPEEVKFKWEDYHNEAMQNAVEEDWDDDWSEEDDEGIEFIYERH"
     gene            5617..6234
                     /locus_tag="PXB27_000994"
     CDS             5617..6234
                     /locus_tag="PXB27_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HDL5345802.1"
                     /translation="MLSSTSCDLFNIPFFQFAQMKKYCPEEIPRIKAEYKTQWEIWKK
                     LHQEVSRQLGQPFAPPHIEKWCNGWQVRAHFFAYYKYEFNRNSAAILSVILNRRRLQV
                     SLDWHCYRADRSQINVHQYNQWWENLDREKFADFEIWHGSESEYDDFRTVSQMSEQAF
                     TLTNEEDFWCIGKNVEKDDLDNIDVVEFIYQTIRALLPLYEKCHQ"
     gene            complement(6281..8239)
                     /locus_tag="PXB27_000995"
     CDS             complement(6281..8239)
                     /locus_tag="PXB27_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HDL5345803.1"
                     /translation="MIAELGNYTLALALAISILLAVLPLWGAEKQNSTLIALARPMTW
                     AMFLALTVSFGSLFYLFAVNDFTVQYVVNNSNSRLPLQYRLSAVWGAHEGSLLLWVWL
                     LSLWSVGVATFTRKMPEDAVARVLGLMGVIGIGFLMFVIFTSNPFDRTFPNFPIDGKE
                     LNPMLQDVGLIFHPPLLYMGYVGFSVAFAFAIASLMTGRLDTAWARWSRPWTMAAWVF
                     LTLGIVLGSWWAYYELGWGGWWFWDPVENASLMPWLAGTALIHSLAVTEKRGTFKAWT
                     VLLAILAFSLCLVGTFLVRSGILVSVHAFASDPTRGLYILAYLVLVVGGSLLLYAFKG
                     SEIKSLDNYERYSRETMLLINNILLMAFLSVVFLGTLLPLIHKQIGLGTISIGAPFFD
                     QMFLILMVPFSFVLGIGPLVKWRRDQVSAIRKPVIIATILMALLGFGLPYLFRNTITV
                     SVVLGTMMSVFIVLLSLYELHQRATHRHSFLSGIFKLSRSHWGMVLAHLGVAMTVFGI
                     AFSQNFSIERDVRMNVGDKAEILDYQFEFKGIKITDGANYQGGTAELEITRNGKYEAT
                     LNAEKRFYTVSRMGMTEAAIDWGFTRDLYAALGEKLEDNSWAVRLYYKPFIRWIWIGG
                     LFMALGGLLCMLDKRYRLRIENKKEVIA"
     gene            complement(8236..8793)
                     /gene="ccmE"
                     /locus_tag="PXB27_000996"
     CDS             complement(8236..8793)
                     /gene="ccmE"
                     /locus_tag="PXB27_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HDL5345804.1"
                     /translation="MNPRRKSRLKVVVSILLGLSVAAGLTLYALSQNIDLFYTPSEIV
                     NGKNDDPKQKPEVGQRIRVGGMVVEDTVKRDDKSLKVVFDLNDIGPSITVEYEGILPD
                     LFREGQGIVAQGVLIEPTRLKATEVLAKHDENYMPPELGDKLKEQHKKSGVSEQDLKG
                     ESETDRKMKAEAEAEKANNVQGESK"
     gene            complement(8979..9176)
                     /gene="ccmD"
                     /locus_tag="PXB27_000997"
     CDS             complement(8979..9176)
                     /gene="ccmD"
                     /locus_tag="PXB27_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HDL5345805.1"
                     /translation="MTFQFESISDFFAMGGYGFYVWLSYGVSFIAMIGLIWLSRREQK
                     AILAQAKKEIVREEKLKQTSQ"
     gene            complement(9191..9928)
                     /locus_tag="PXB27_000998"
     CDS             complement(9191..9928)
                     /locus_tag="PXB27_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HDL5345806.1"
                     /translation="MWKWLHPYAKSETQYHLLGKIQPFLGWLSLIMLAAAFVWGLAFA
                     PKDYQQGDSYRIIFIHVPAAIWSMGVYGSMAVAGLVALVWQIRQANLAMISMAPIGLV
                     FAFISLATGAIWGKPMWGTWWVWDARLTSALVLFFLYIGVMALYSAFQDRQTGAKAAA
                     VLSVVGVINLPIIHFSVEWWNTLHQGATITKFDKPSMAVEMLIPLLLAIFGSLIFTAW
                     FSIWRYRIALLKDERNRPWVKQLAKSV"
     gene            complement(10128..10790)
                     /gene="ccmB"
                     /locus_tag="PXB27_000999"
     CDS             complement(10128..10790)
                     /gene="ccmB"
                     /locus_tag="PXB27_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005565617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HDL5345807.1"
                     /translation="MILLTIIQRELTIAFRKPSEILNPLWFFLMVITVFPILMGPNPE
                     LLGKIAGGIAWVAALLSALLSFERLFRDDYLDGSLEQLMLLPLALPQVALAKVIAHWL
                     LTGLPLILLSPIAAILLSLETHVWWALVLTLLLGTPILSCLGAIGVALTVGLRKGGTL
                     LSLLILPLFLPVLIFAAAVLEAATLNMGYSGQLAIIGAILAVTLTFSPFAIAGALRIS
                     LN"
     gene            complement(10787..11428)
                     /gene="ccmA"
                     /locus_tag="PXB27_001000"
     CDS             complement(10787..11428)
                     /gene="ccmA"
                     /locus_tag="PXB27_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HDL5345808.1"
                     /translation="MNQTAQYQLKLNQIACERGETRLFEGCSFTVQSGEWVQIEGHNG
                     IGKTSLLRILAGLFLPAEGEVLWNDIAIQKQRDHYYSELFYLGHYSGVKPELTPWENL
                     RFYQKIQQLPQDDDALWHALTKVSLIGREDLPCSHLSAGQQRRVALAKLWLTNAKLWI
                     LDEPFTAIDKKGVADLIAHIEQHCEQGRLVIFTSHQAAESDKVRIISLDQFKV"
     gene            complement(11572..12282)
                     /gene="hda"
                     /locus_tag="PXB27_001001"
     CDS             complement(11572..12282)
                     /gene="hda"
                     /locus_tag="PXB27_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HDL5345809.1"
                     /translation="MQLPLPIHQIDEDTFDNFYSQNSEVLLGSLKRNFTDVQQPFFYI
                     WGGKSCGKSHLLKAVSNHFLLNNQTSSYIPLTKANYFSPLVLDNAELLNVICLDDIQA
                     IAGDAEWELAIFNLFNQIREQQGLFNEGQKTLLLISADCPPHQLNIQLPDLRSRLTWG
                     EVYQLNDLTDEQKCQILQLNAHQKGVELSDEVAHFLLKKAGTDLEQLSKILDKLDKAS
                     LQAQRKLTIPFVKEILSL"
     gene            12552..14153
                     /locus_tag="PXB27_001002"
     CDS             12552..14153
                     /locus_tag="PXB27_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5345810.1"
                     /translation="MKNMTKLSLITLALAASSSVFAAPKTFVYCLEASPSALSPVLST
                     DGASFDVNALPIYNKLTDFEDGTTNVIPSLAEKWDISEDGKTYTFHLRKGVKFHDNKE
                     FKPSRELNADDVVFSINRQFDPNHPFHKVSGGNYEYFIGMDMQNIIDKVEKVDDHTVK
                     ISLKVPNAPFLANLAMDFNAVYSAEYAEQSLKAGKPERIDTNPIGSGPFQFVDYQKDA
                     TVRYKAFENYWQGTAKIDRLVFAITPDASVRLAKLQKGECHAMPYPNPADIEDLKKDP
                     NIELMSQSGMNIGYLALNSSIKPLDNQKVRQALNHAVNKQAIVDAVYQGAGQVAKNPI
                     PPTMWSYNEAVQDYDYNPEKAKALLKEAGFENGFDTELWAMPVSRPYNPNARRMAELI
                     QEDWKKVGVNAKIVSYEWGEYLKRLRQGEAATSMIGWTGDNGDPDNFLNTLLSCSAVE
                     AGSNYAKFCHKPFEEVVLGAAQESDKAKRTELYKQAQVIFKEQAPWITIAHSTVYFPV
                     RKEVKGYVMSPFSLHNFYKVDLADK"
     gene            14425..15429
                     /locus_tag="PXB27_001003"
     CDS             14425..15429
                     /locus_tag="PXB27_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005712947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL5345811.1"
                     /translation="MFSFILKRVLMVIPTFIAITLITFALVHFIPGDPIEIRMGERGL
                     SPEVHQQMMEQLGLNRPLFEQYISYIKGVLHGDLGNSFRNNEPVLKEFFTLFPATVEL
                     AFFALLWSLIFGIILGVIAAVKKESWISHTVTTLSLTGYSMPIFWWGLILILYFSTPL
                     GLPASGRLPSEYWIEAETGFMLIDTWNSDEPGAFLAAIKSLILPSIVLGTIPLAVVTR
                     MTRSSMLEVLGEDYIRTAKAKGLNTTRIVIVHALRNALIPVVTVVGLIVGQLLSGAVL
                     TENIFSWPGIGKWIIDAINSRDYPVLQGSVLIIATIIILVNLTVDLLYGIVNPRIRHS
                     "
     gene            15531..16424
                     /locus_tag="PXB27_001004"
     CDS             15531..16424
                     /locus_tag="PXB27_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL5345812.1"
                     /translation="MSGITAVNPPAPKTPLQEFWYYFCQNRGALIGLTFIAIVFVACV
                     FAPWVAPFDPIEQNRSALLLPPAWLEGGNSAHLLGTDDIGRDILSRIIYGSRLSVFIG
                     LVIVLLSCVFGVILGLLAGYYGGTLDIIIMRFVDIMLAIPSLLLTIGVVTILGPSLIN
                     AAIAIAIVSIPSYVRLTRASVLSEKNRDYVTASRVAGASVLRLMFVVILPNCLAPLIV
                     QMTMGISNAILELAALGFLGIGAQPPTPELGTMLAESRGFMQSANWLVTIPGLAILSL
                     VLAFNLMGDGLRDALDPKLKQ"
     gene            16435..17424
                     /gene="dppD"
                     /locus_tag="PXB27_001005"
     CDS             16435..17424
                     /gene="dppD"
                     /locus_tag="PXB27_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HDL5345813.1"
                     /translation="MALLTVDQLSVHFGETKTPFKAVDRISYEVNEGEVLGIVGESGS
                     GKSVSSLAIMGLIDFPGRVMAEKLYFNGNDLLALKPKEKQQIVGADVSMIFQDAMTSL
                     NPSYTVGYQIMEALKVHQGGSKASRRERAIELLTMVGIPDPESRLEVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIDLLLELQRKENMALILITHDLALVA
                     ESAHRIIVMYAGQVVEEGKAEQIFKAPLHPYTQALLKALPEFAEGKSRLQSLPGVVPG
                     KYDRPQGCLLNPRCPYATEKCRTIEPAMQIINGRQVKCHTPLNAQGEPIRIAV"
     gene            17552..18541
                     /locus_tag="PXB27_001006"
     CDS             17552..18541
                     /locus_tag="PXB27_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5345814.1"
                     /translation="MKENNSLPPFLKAVDLKKYYPVKKGMFAKPKTVKALDGVSFTLE
                     RGKTLAIVGESGCGKSTLGRMLTMIESPTEGELFYNGQNFLVNDKETAQLRRKKIQIV
                     FQNPYSSLNPRKKVGSILEEPLLINTNLSAKERKEKVLEMMAKVGLKPEFYDRYPHMF
                     SGGQRQRIAIARGLMLQPDIVVADEPVSALDVSVRAQVLNLMMDLQEEMGLSYVFISH
                     DLSVVEHIADEVMVMYLGRCVEQGDTKTIFSNPRHPYTQALLSATPRLNPEQRRERIK
                     LTGELPSPLNPPKGCAFNARCRFATDRCYVERPELKTYQDGTRIACFVVEENN"
BASE COUNT         5668 a         4141 c         3942 g         5081 t
ORIGIN      
        1 aaaggaatca gactatgagc caacttgaca ccttaagcaa aatgaccgtt atcgttgctg
       61 acactggtga tattgaagca atcaaacttt ataagccaaa agatgcaaca acaaacccgt
      121 ctttaatttt aagtgcatcg gcattaccgc aatatgctcc attaattgaa gaagcgatta
      181 attacgcaaa agcacaaagt agcgataaag atcaacagtt agttgatgct gaagataaat
      241 tagcggtaaa tattggttta gaaatcttaa aacttgtacc tggacgtatt tctacagaag
      301 ttgatgctcg tttttcttat gatactgaaa aaactatcgc taaagcacgt aaattaattg
      361 gattatataa cgatgcaggc atcagtaatg atcgcatttt aattaaaatt gcctctactt
      421 ggcaaggtat aaaggcagca gaacagttag aaaaagaagg cattaactgt aacttaacgt
      481 tattattctc tcaagctcaa gctcgtgcgt gtgcagaagc aggggtttac ttaatctctc
      541 cattcgttgg tcgtatttta gactggtaca aagctcacga aggtaaagaa tatgctccag
      601 ctgaagatcc aggtgtaatt tcagtaacca atatatacaa ttactacaaa caacacggtt
      661 atgacactat tgtaatgggt gcaagtttcc gtaatgtagg agagatcatt gagttagctg
      721 gttgcgaccg tttaaccatc tcaccaccgt tattaaaaga gctacaagag agcgatgaaa
      781 aattagtttg caagctagat tacaaaggtg aaatcaagcc tcgtccagaa aaaatgacag
      841 aagcagaatt ctactgggag cataaccaag agccaatggc agtagaaaaa ttagctgaag
      901 gcattcgtaa atttgcgatt gaccaagaga agttagaagc aatgcttgct gaaaaacttc
      961 aatagacaac gcataaaaaa accggcatta acgataatta atgccggttt taatttctat
     1021 aaatgattat ttaaactcat aacgtctaat cgtttttaga tcgctatctt catttttaat
     1081 ttcatcggca agatgaacac gagcaccaac cgctgcatgt gcatcatact cacgagttag
     1141 agtcacagtt tcaccattat ctcgttgaac tgtaagttca acaacatcgt ttgatactgc
     1201 tttgctagat aaatgttgca ttacctttat cagaataaac atctgcagta caagctgcta
     1261 acataagtga agctaataca gctaaagaaa gttttttcat taataatata tcctctagga
     1321 aaaactaaaa ttaaagcaga gaaacattca ggtctgaatt agaccctacg attctcacac
     1381 gtttgccagg cacaaaaccg tcttctttct tttgaacgac cacgatttct ttaccatcat
     1441 ctttgcgaat aaccatttcc aatgaagaaa cttgactcgc tttttgttct acttgattac
     1501 caattaccgc accggcaact gcccccactg cggttgcaat cgcttgccct gtaccaccgc
     1561 caatcgcatt accggcaacg ccacctaata caccaccgcc gacagtaccg atcacacctt
     1621 cactgcgtgc ttgaattttc acatcacgca ctgaaacaat agtgccataa gcgatagaac
     1681 gagcttcttt tgcctgctca ccggaataaa cgcttccgct aaacacgtct gtatttgcac
     1741 agccaactaa agcaaaactt gacattaatg cgacagctaa agctaatttt ttcattttgt
     1801 ttgttctccg aaacgttaat tttctgacat aattacactt tttatatcat aagggtggag
     1861 ctttagacaa taaggattac aaaaagttac atttctcaag ctgatcgcaa tgcttggatt
     1921 tggcaatctc tccccttcta cctgaggttg gctgattttt aatttaatct cagaattatc
     1981 agcaagtcta aagtgcgaca atgcccttga cacccattgt tccgaacttt cgttgtctaa
     2041 catcggaaaa accagctcaa tatgttccca gccttcttct aaataaacct tatttttcgg
     2101 aaaaggcaac tcgacaatct ttacaaattg tccgcaaaat tcaaccgctt gtattaatgc
     2161 aaacagccct atcggtctgc cgtttacctc gctctcttta agtaaggttg ccccatcaag
     2221 caaaaggatc ttccactgtt ctgcggtttc ggtttggttc attcttaccg caagatgatc
     2281 gatttcatac gctgctaaat caatgccggc gatttcagca atttgtcgga ttttctgttc
     2341 aaattgcgat aaatcgccaa aacaagcggt cattttctcg aaaaattttg cattttctgc
     2401 tataattttg tccatttttt tgtaaataga atttaaatag gttaaacagt gaatattcaa
     2461 caaattttat cagataaaat taaacaggca atggtagcaa gcggtgcaga cagcaatgtc
     2521 gagcctttag tccgccaatc gggcaagccg gaatttggcg attaccaagc caatggtgcg
     2581 atgggtgcag cgaaaaaatt ggggatgaat ccgcgtgaat ttgcccaaaa aatcttagag
     2641 aatgccgatt tgaacggtat tgccgataaa ttagaaattg ccggtccggg ctttatcaac
     2701 attttcttaa acccagaatg gctggcggag aacactaaca gttcattgca agcggtcaat
     2761 tttggcataa aaactgcaaa tccgcagacg attgtgatcg actactcctc gccaaacgta
     2821 gcaaaagaaa tgcacgtcgg gcatttgcgt tccaccatta ttggcgatgc ggtagttcgt
     2881 acccttgcgt ttttaggcaa taatgtgatt cgtgccaacc acgttggcga ctggggtact
     2941 caattcggta tgctgatcgc ttaccttgaa aaaatggaaa atgagcacgc cagcgaaatg
     3001 gagctaagcg atttagaagc cttctatcgt gcggcaaaag agcattatga ttcagacgaa
     3061 gcattcgccg aaaaagcccg taactatgtg gtgaaattac aaagcggcga taaatattgc
     3121 ttaacaatgt ggaaaaaatt ggtcgatatt acaatgcacc acaatcaaga gaactacaat
     3181 cgcttaaatg ttacgctcac cgaaaaagat gtaatgggcg aaagcctcta caatccaatg
     3241 ttgcctgaaa ttgtggcgga tttaaaagcc aaaggcttag cggtggaaga tgacggtgct
     3301 ttagtggttt tcctcgatga atttaaaaat aaagatggcg acccaatggg cgtaatcgtg
     3361 cagaaaaaag atggcggttt cttatatacc actaccgata ttgcggcagc aaaatatcgc
     3421 tacgagacct taaaagctga ccgagcctta gtcttttctg acagccgcca agctcaacat
     3481 atgcaacagg catggctcat tacccgcaaa gcgggctatg tgccggacag tttcagcctt
     3541 gaacacccgt tcttcggaat gatgctaggc aaagacggca aaccgttcaa aacccgtagc
     3601 ggtggcacag tcaaactcaa agatttatta gatgaagcgg tagagcgtgc cgataaactg
     3661 attgccgaac gttcaaccga gctaactgcg gaagaaaaag ccgccgtggt tgaagcggtt
     3721 gcgattggtt cggtgaaata ttctgactta tcgaaaaacc gcaccaccga ttatgtgttc
     3781 gattgggaca atatgctgac ctttgaaggc aataccgccc cgtatatgca gtatgcctac
     3841 acccgcattc gctccatctt tgcccgtgcg aacattgacg aaaatgcatt gagcggttca
     3901 atccaactca atgagccaaa agagcgtgca ttagcagtga aactattgca atttgaagag
     3961 gcattaaacg gcgtggcaaa agaaggtatg ccacatattt tatgccaata tttatacgag
     4021 cttgcaggta gtttctccag tttctacgag gcttgcccga ttctcaatgc cgaagaaaca
     4081 accaaaaaca gccgtttgaa actcgctgcc ttaacggcga aaaccttgaa acaagggttg
     4141 gatttactcg gtattaaaac tgtagagaag atgtaataca aactctctcc ccttgtggga
     4201 gagagaccgc ttatgagcgt tcagcgaaat aagcagagag aggggacaag cggtaatttt
     4261 tattaaaatt tttacacatt ttcccctctc cctgattttt cttctgaacg aagaaaaatc
     4321 tgtccctctc ccacaagggg agagggcata taaaaggaaa caaatatgaa atttattgac
     4381 gaagccctga ttcgtgtaga agcaggcgat ggcggtaatg gctgtgtgag cttccgccgt
     4441 gaaaaattta tcccgaaagg cggacctgac ggtggtgatg gtggcgatgg tggtgatgtg
     4501 tatttaattg ccgatgaaaa cctaaacacc cttatcgact accgctttga aaaacgttat
     4561 gccgctgggc gtggcgagaa tggtcgcagt gccggctgta ccggacatcg tgggcaagat
     4621 attaccctac gagtgccggt tggaacccgt gcgattgata acgacaccca agaaattatt
     4681 ggcgatttaa cccgccacgg aatgaaaatg ctggtggcaa aaggtggtta tcacggttta
     4741 ggtaataccc gctttaaatc ctcagtaaac cgagctccac gccaaaaaac caacggtacg
     4801 cctggcgaaa aacgtgattt gaaattagag ctgttactgc ttgccgatgt gggaatgctt
     4861 ggcttaccaa acgccggtaa atccaccttt atccgcagtg tctctgctgc caaaccaaaa
     4921 gtggcggatt acccgttcac gacgctggtg ccaagtttag gggtagcccg tgtgggggcc
     4981 gaccgcagtt ttgtggttgc cgatattccg gggttaattg aaggtgcagc agatggtgca
     5041 gggctgggga ttcgtttcct aaaacacttg gaacgttgcc gtattttgat tcacttagtc
     5101 gatattatgc cgattgatga aagcgatccg gctcataata tttcggtgat tgaatccgag
     5161 ctttaccaat acagcgaaaa actcgccgaa aaaccgatct ggttggtgtt taataaaatt
     5221 gacaccatcg gcgaagaaga agctgctgag cgagcaaaag acattgccga gcaaatcggc
     5281 tgggaaggcg attactactt gatttctgcc gctaccggtc aaaacgtgca agctctcact
     5341 cgtgatatta tggactttat tgaagccaac ccacgcgaag tggaggaaga aaataaagca
     5401 cctgaagaag tcaaattcaa atgggaagac taccacaacg aagcaatgca aaatgcggta
     5461 gaagaagatt gggacgacga ttggagcgaa gaagatgacg aaggcataga gtttatttac
     5521 gaacgccatt agtgcaataa tacaagcggt tgtttttaaa cggagatttg caaatttccg
     5581 gagaaaaaca accgcttgta ggtttaagga gagtttatgc ttagctcaac atcgtgcgat
     5641 ttattcaata ttccgttctt ccaatttgct cagatgaaga aatattgccc tgaggagatc
     5701 cctcgaatta aggcggaata taaaactcaa tgggaaattt ggaaaaagct acatcaggaa
     5761 gtttctcgtc agcttgggca gccgtttgct ccaccacata ttgagaagtg gtgcaacggt
     5821 tggcaagttc gagcccattt ttttgcctat tacaagtatg aatttaatcg aaattctgcg
     5881 gctattcttt ccgtgatttt aaatcgtcgc cgtttgcaag tgagccttga ttggcattgt
     5941 tatcgagccg atcgctcgca aattaacgtg catcaataca atcaatggtg ggaaaattta
     6001 gatcgggaaa agtttgctga ttttgagatt tggcacggaa gtgagagtga atatgatgat
     6061 tttagaaccg ttagtcagat gtcagagcaa gcgttcaccc taactaacga ggaagatttt
     6121 tggtgtatcg gcaaaaatgt ggaaaaggac gatttggata acattgatgt agtagaattt
     6181 atctaccaaa ccattcgggc attattgcca ctttatgaga aatgtcatca ataaacacta
     6241 aaaagagagg cgaacatcat ttgttcgccc ctttcaaaat ttaagctatc acttcttttt
     6301 tattttcaat gcgtaaacgg tagcgtttat ccaacataca gagtaaacca ccgagagcca
     6361 taaacagccc gccaatccaa atccagcgaa tgaacggttt ataatacaaa cgcactgccc
     6421 aagagttatc ttctaatttt tcgccaagtg cagcgtataa atctcgggta aagccccagt
     6481 caatcgccgc ttcggtcatt cccattcggc tcaccgtata gaaacgtttt tcagcattaa
     6541 gtgtggcttc atatttaccg tttcgagtaa tttcaagctc ggcagtgccg ccttgatagt
     6601 ttgccccatc ggtgatttta atacctttaa attcaaattg gtaatctaaa atctcagctt
     6661 tatcacccac attcattctg acatctcgtt caatgctgaa attttggcta aatgcaatgc
     6721 cgaacactgt catcgcaacc cctaagtgag ccagcaccat tccccaatgt gaacgggaca
     6781 gtttgaaaat gccacttaag aacgagtggc gatgggttgc acgttggtgt agctcataca
     6841 ggctaagtaa cacaataaag acagacatca tcgtgcctaa caccacactc acggtaatgg
     6901 tgttacggaa taagtatggc aatccgaatc caagcaatgc cattaaaata gttgcaataa
     6961 ttaccggttt acgaatagca gaaacttggt ctctacgcca tttcactaat ggtccaatcc
     7021 ctaatacaaa tgagaaagga accattaaaa ttaagaacat ttggtcaaaa aatggtgccc
     7081 cgattgaaat cgtccctaag ccgatttgtt tgtgaattaa tggcagtaag gtccctaaaa
     7141 acaccaccga taaaaatgcc attaataaaa tattattaat gagtagcatc gtttctcttg
     7201 aatagcgttc atagttatct aagcttttaa tttccgagcc tttgaatgca tacagcagta
     7261 aagaaccacc tactactaac accaagtaag ccaagatgta taaaccacga gttggatctg
     7321 aagcaaaggc gtgaactgac accaaaatgc ctgaacgcac taagaaagtc cccactaaac
     7381 agagcgagaa agcaagaatc gctaataaga ccgtccacgc tttaaatgtg cctcgttttt
     7441 cagtcactgc taatgaatga atcagtgctg ttcctgcgag ccacggcatt aatgaggcat
     7501 tctcgacagg atcccagaac caccagccac cccaaccaag ttcgtagtat gcccaccaag
     7561 aaccaagcac gataccgagc gttaaaaata cccacgctgc cattgtccac ggacgagacc
     7621 atcttgccca cgcagtatct aaacgccctg tcatcagtga ggcaatcgca aaagcaaagg
     7681 caacggagaa acccacatag cccatataaa gcaacggtgg gtggaagatc aagcccacat
     7741 cttgcagcat tgggtttaat tctttgccat caatcggaaa atttgggaaa gttctatcaa
     7801 acgggttgga ggtaaaaatc acaaacatca aaaagccgat gccaatgacc cccattagtc
     7861 ctaacactcg agcaacggca tcttccggca ttttacgggt aaaggttgct accccgacag
     7921 accaaagcga caacaaccaa acccaaagca ataatgagcc ttcgtgagcg ccccacaccg
     7981 cagataaacg atattgcaac ggtaaacggc tattggagtt gttcaccaca tattgcacgg
     8041 taaaatcatt caccgcaaac agataaaaca gcgaaccaaa agagacagtc aatgccaaaa
     8101 acattgccca agtcatcggg cgggcaagag caattaaggt gctattttgc ttttctgctc
     8161 cccaaagcgg taaaactgcc aacaagatag aaatcgctag ggctaatgcc aacgtataat
     8221 tgcctaattc tgcaatcatt tgctttcccc ttgcacattg ttggcttttt ctgcttccgc
     8281 ctcggctttc attttgcggt cggtttccga ttcacctttc agatcttgtt cagacacacc
     8341 tgatttttta tgctgttctt ttaatttatc gcctaattcc ggtggcatat agttttcatc
     8401 gtgttttgcc agaacttccg tagcttttaa acgggttggt tcaatcaaca cgccttgtgc
     8461 cacaatgcct tgcccttcac ggaataaatc cggcaaaatt ccttcgtatt ccacggtaat
     8521 cgaaggcccg atatcattga gatcaaatac tacttttaaa cttttatcat cacgctttac
     8581 cgtatcttcc actaccattc cgcccacacg gatacgctga ccaacttcag gcttttgttt
     8641 cggatcatca tttttaccgt ttacaatttc agacggcgtg taaaacagat cgatattttg
     8701 gcttaacgca taaagtgtca agcctgctgc gacagaaagc ccaagtaata tcgaaaccac
     8761 cacttttaat cttgatttgc gtcttggatt catcgtaatc acccttcaaa ataaatgcaa
     8821 aattgcataa aaactcaaaa ataaattagt gtaagaatag caaaaaaccg caaaacattc
     8881 ccgatatttc aaggaagttt tgcgatttct caaatcaaat acctataaag aagtatgagg
     8941 ctatcgttgc aaaaaataaa gttatttcga ccgcttgttt attgtgatgt ttgctttaat
     9001 ttttcttcac gcacaatctc tttttttgct tgagccaaaa tcgctttctg ttcacggcga
     9061 ctaagccaaa tcagcccaat cattgcaatg aaactgacac cataagagag ccatacataa
     9121 aagccgtagc cacccattgc aaaaaaatcg ctgattgatt caaattgaaa tgtcattgtt
     9181 ctctcctact ctaaactgat tttgccagtt gttttaccca agggcgatta cgttcgtcct
     9241 ttaataaggc aatgcgataa cgccaaatgc taaaccaagc ggtaaaaatt aagctaccga
     9301 aaatcgccaa tagcaacgga attaacattt ccaccgccat agacggttta tcgaatttag
     9361 tgatggtcgc cccttgatgc aaggtgttcc accattccac cgaaaagtga ataatcggca
     9421 agttaatcac gccaacaact gacagcacag ccgccgcttt ggctccggtt tgtctgtctt
     9481 ggaatgcgga ataaagagcc atcacgccga tataaaggaa aaataacact aaagcggagg
     9541 ttaaacgagc gtcccacacc caccaagtcc cccacattgg tttgccccaa attgcaccgg
     9601 ttgcaagtga aataaaagcg aatactaagc cgataggtgc cattgaaatc atcgccaagt
     9661 tagcttggcg aatttgccaa accaatgcca ccaagcctgc caccgccatt gagccatata
     9721 cgcccatcga ccaaattgct gccggcacgt gaataaagat aatgcgatag ctatcgccct
     9781 gttggtaatc tttcggggca aaagccaagc cccaaacaaa agcagccgct aacataatca
     9841 aacttagcca acctaaaaac ggttggattt tccctagcaa atggtactgc gtttccgatt
     9901 ttgcataagg gtgtagccat ttccacatat atttctccaa agtaaataat tcccctcacc
     9961 cgtttacagg agagggacga tttgagcttc gttcagaagc gaaaatcagg gagaggggaa
    10021 aaagttgcaa attttcataa taattcgacc gcttcccctc tctctgaaaa acacctctga
    10081 acgaggtgtt tttcggtctc tctccctcaa gagggagaga ggaagtttta gttcaaacta
    10141 attcttaatg caccggcaat cgcaaacggc gaaaaagtta aagtaaccgc taaaattgct
    10201 ccgataatcg ccagttgtcc gctataaccc atatttaagg tggcggcttc taataccgca
    10261 gcggcaaaaa tcagtaccgg taagaataac gggaggatta ataaactcag caacgtaccg
    10321 cccttgcgta agcccacagt gagtgccacg ccaatcgctc ctaagcagct taaaatcggg
    10381 gtgccgagta ataatgtcag cactaacgcc caccaaacgt gggtttcaag tgaaagcaaa
    10441 atggcagcaa tcggtgaaag taaaatcaac ggtaagccgg tcagcagcca gtgagcaatc
    10501 actttcgcta acgcaacctg cggcaaggca agcggcagta acattaattg ctcgagcgaa
    10561 ccgtctaaat aatcatcacg gaataaccgc tcaaaggaaa gcaatgcaga aagcaaagca
    10621 gctacccacg caatgccgcc ggcaattttg cctaataatt cggggttagg tcccattaaa
    10681 atcgggaata cggtaatcac cattaaaaaa aaccacaacg gatttaaaat ctccgagggt
    10741 ttacgaaagg caatggtgag ttcccgctga ataatagtca ataaaatcat actttaaatt
    10801 gatctaacga aataatacgc actttgtcgc tttcagcagc ctggtggctg gtaaaaatca
    10861 ctaatctgcc ttgctcgcaa tgctgttcaa tatgagcaat cagatccgcc acaccctttt
    10921 tatcaatagc ggtaaagggt tcatctaaaa tccaaagttt ggcgttagtg agccacaatt
    10981 ttgccaacgc cacacggcgt tgctgaccgg ctgacaaatg cgaacaaggc aaatcttcac
    11041 gcccgatgag cgacactttc gtcaatgcgt gccaaagggc atcatcatct tgcggtaact
    11101 gttggatttt ctggtagaaa cgcaaatttt cccacggtgt taattccggt tttacccccg
    11161 aataatgccc taaataaaat agttcagaat aatagtgatc acgctgtttt tgaattgcaa
    11221 tatcgttcca cagcacctca ccttccgcag gtaaaaaaag cccggctaaa atacgtaaca
    11281 ggctggtttt tccaatgccg ttatgccctt caatctgcac ccattcacca ctttgcacgg
    11341 taaaactgca cccttcaaac aaacgggttt cgccccgttc gcaggcaatc tgattaagtt
    11401 taagctgata ttgggccgtt tggttcatta tttttcatct ggttgaacat tttagccgaa
    11461 gtgtatcata agcctttgat ttaacgtagc aaataaatta cttctttaga ggtagcaagc
    11521 ggtcaaattt tcaaaaagtt ttgcaaattt tgataagaat caaaccgctt gttacagaga
    11581 gaggatttcc ttcacaaaag gaatggttaa tttacgctgg gcttggagag acgctttatc
    11641 caacttatcc aatattttag agagttgctc taaatccgtt cccgcttttt ttaacagaaa
    11701 atgagcgacc tcatcggaca attccacccc tttttggtga gcatttaact gtaaaatttg
    11761 gcatttttgt tcatcggtta aatcgtttag ctggtacact tcgccccaag ttaagcggga
    11821 acgtaaatcc ggcaattgga tattgagttg gtgtggtggg caatcggcac tgattaacag
    11881 caaggttttt tgcccttcgt taaacaagcc ttgttgttct cgaatttggt taaacaggtt
    11941 aaaaatagca agttcccatt cagcatctcc tgcaatcgct tgaatatcat ctaggcaaat
    12001 cacattcagc agttcggcat tatctaacac caacggcgag aaataattgg ctttagtgag
    12061 cggaatataa ctagaagttt ggttatttaa aagaaagtgg ttacttactg ctttcaacaa
    12121 atgactttta ccgcagctct taccgcccca aatgtaaaaa aacggctgtt gaacatccgt
    12181 aaaatttcgt ttgagggatc caagcaacac ttcgctgttt tggctataaa aattgtcgaa
    12241 agtgtcttca tcaatttgat gaatgggtag aggtaactgc aaggctttcc ttaaatctct
    12301 aagtttgata tttttctaag aatagcggaa aagcaggggt ttgagaagta aaaatagtga
    12361 gaaaatatcc actcattttt ctaatgaaga gatgacaagc ggttgaattt ccacataatt
    12421 ttgtcaatct gaccgaaaac ccaccgtttt gtgttaaaaa aagattgcag ttagctcaaa
    12481 aaattgtata ttcaaagctt tctacttaaa aagtagagta aaacaactat tacaaacaca
    12541 acggagtgaa tatgaaaaac atgactaaat tatcgctaat aacattagcg ctagccgctt
    12601 caagtagcgt ctttgctgca ccgaaaacct ttgtttactg cttagaggct tcgccttcag
    12661 cattaagccc agtgctatca acagacggtg caagctttga tgtgaacgcc ctcccgattt
    12721 ataacaaatt aaccgacttt gaagacggta caactaatgt tattccctct cttgccgaaa
    12781 aatgggacat ttcggaagac ggcaaaactt acaccttcca cctgcgtaaa ggcgtaaaat
    12841 tccacgacaa taaagaattt aaaccaagcc gtgagttaaa tgcagacgat gtagtgttct
    12901 ccattaaccg tcagtttgat ccaaaccatc cattccacaa agtttccggc ggtaactacg
    12961 aatactttat cggtatggat atgcaaaata ttatcgataa agtggaaaaa gtggacgatc
    13021 acaccgttaa aatcagctta aaagtgccaa atgcgccgtt cttagctaac ttagcgatgg
    13081 atttcaacgc cgtttactca gcggaatatg cagagcaatc cttaaaggcc ggcaagcctg
    13141 agcgtattga taccaaccca atcggcagcg gcccgttcca gtttgtggat taccaaaaag
    13201 atgccaccgt tcgttacaaa gcctttgaaa actactggca aggcacagcg aaaattgacc
    13261 gcttagtgtt tgcaattact ccggatgcat cggtacgttt agccaagtta caaaaaggcg
    13321 agtgccacgc aatgccttac cctaacccgg ctgatatcga agatctgaaa aaagatccaa
    13381 atattgaatt aatgagccaa tctggaatga atatcggcta tttggcatta aattcatcga
    13441 ttaaaccatt agataaccaa aaggtacgtc aagcattaaa tcacgctgta aacaaacaag
    13501 cgattgtaga tgcggtatat cagggagccg gtcaagtagc gaaaaaccca attccaccaa
    13561 caatgtggag ctataatgaa gcggttcaag actatgacta caaccctgaa aaagccaaag
    13621 cattgttaaa agaagccggt tttgaaaatg gttttgatac tgaactttgg gcaatgccgg
    13681 tatcacgtcc atataacccg aatgctcgcc gtatggctga acttatccaa gaagactgga
    13741 aaaaagtggg cgtgaatgcg aaaatcgtca gctatgaatg gggcgaatat ctcaaacgct
    13801 tacgccaagg tgaagcggca acctcaatga ttggctggac aggcgataac ggtgacccag
    13861 ataacttcct caacacctta ttaagctgct cagcggtgga agccggttca aactacgcca
    13921 aattctgcca caaaccgttt gaagaagtcg tgcttggtgc tgcccaagaa agcgacaaag
    13981 caaaacgtac cgagctttat aaacaagctc aagtgatctt caaagagcaa gctccttgga
    14041 ttacgattgc tcactccacc gtttacttcc cggtgcgtaa agaagtaaaa ggttatgtga
    14101 tgagtccgtt cagcttacat aacttctaca aagtagattt agcggataaa taatccgcca
    14161 acaagcggta gaatttgcag atttttttac aaatttgacc gcttgtcctc ccctctttga
    14221 actacgaggg gcagggggga aatttggcag aagcgattgc cgaagtgaga ggattgtaaa
    14281 agaggaaata aattttctgt cttattgatt attttcttcg ttaattacta ctgtcaaatc
    14341 tcccctaccc cctctttgct aaagagggga atcagatcga gagatcagac aactttgatg
    14401 aaaatccaac ctaagagatt ttaaatgttt tcatttattt taaaacgtgt gctaatggtg
    14461 atccctacct ttatagcgat tacgttaatt acttttgccc ttgtgcattt tatccccggt
    14521 gatccaattg agatccgaat gggggaacgt ggattatcgc ctgaggttca tcaacaaatg
    14581 atggaacaac tcgggctgaa ccgaccgttg tttgaacaat acatcagcta catcaaaggt
    14641 gtattgcacg gtgatcttgg taactcattt cgtaacaacg aaccggtgct gaaggaattt
    14701 tttaccctat tcccagccac ggttgagttg gcattttttg ccctgctctg gtcgttgatt
    14761 ttcggcatta ttctcggtgt gattgctgcg gtgaaaaaag aaagttggat ttcacacact
    14821 gtaaccacgc tttcactgac cggctactca atgccaattt tctggtgggg cttgatttta
    14881 attctttatt tctccacccc gctcggctta ccggcttcag gtcgtttacc atctgaatac
    14941 tggattgaag ccgaaaccgg ttttatgctg attgatactt ggaattcgga cgaaccaggg
    15001 gcatttttag cggcaattaa atctctgatt ttgccgtcta tcgtactggg gacaatccct
    15061 cttgcagttg tgacccgaat gactcgctct tcaatgttgg aagtgttggg tgaagactat
    15121 atccgcaccg ccaaagccaa aggcttaaac actacccgaa ttgtgattgt tcacgcatta
    15181 agaaatgcct tgattccggt ggtaacggtc gttggcttaa ttgtcgggca actgctttca
    15241 ggagccgttt taacggaaaa catcttctcg tggccgggta tcggcaaatg gattattgat
    15301 gcaatcaaca gccgtgatta ccctgtacta caaggctcag tgctgattat tgctactatt
    15361 attattttag tgaaccttac ggtagatcta ctctatggca ttgtcaatcc gaggattcga
    15421 catagttaga aaaattcctt cgatttttcc cctctttagc aacgagggag ttacacaagc
    15481 ggttcaaatt ccgcaatttt ttacaagatt atttttagtt ggagttaatg atgtcaggaa
    15541 taacggcggt aaatccaccc gcacccaaaa caccgttaca ggagttttgg tactattttt
    15601 gtcaaaatcg gggggcatta ataggactta cctttattgc aatcgtcttt gttgcgtgcg
    15661 tattcgcacc ttgggttgcc ccttttgatc caatcgaaca aaatcgcagt gcgttattgc
    15721 tccctcctgc ttggcttgaa ggggggaatt ccgctcatct gctgggtact gacgatattg
    15781 ggcgtgatat tctttcccgt attatttatg gttcaagatt gtccgtcttt atcggcttag
    15841 tcattgtgct actttcttgc gtattcggcg tgattttagg tctacttgct ggctactatg
    15901 gtggcacact cgacatcatc attatgcgtt ttgttgatat tatgctggcg attccaagcc
    15961 tgcttttaac tatcggcgtg gtcactattt taggcccttc attgataaat gccgctattg
    16021 cgattgcgat tgtctccatt ccaagttatg ttcgcttgac ccgtgcttct gtattaagcg
    16081 agaaaaaccg tgattatgtt actgcttctc gtgttgctgg tgcgagcgtg cttcgcttaa
    16141 tgtttgtggt gattttacct aactgtttag ctccgcttat cgttcagatg acgatgggga
    16201 tctcaaacgc tattttagaa cttgccgccc tcggcttctt aggcattggt gcacagccgc
    16261 caacaccgga gcttggcaca atgcttgccg aatctcgtgg ctttatgcaa tcggcaaact
    16321 ggctggtgac cattccgggt ctagcgattt tatcgttagt attagctttc aacttaatgg
    16381 gtgacggctt gcgtgacgcc ctcgatccaa aactgaaaca ataggagatc taaaatggca
    16441 ttattaacgg ttgatcaact ttccgtccat tttggtgaaa ctaaaacacc atttaaagcg
    16501 gtggatcgta tcagttacga agtcaatgaa ggcgaagtat taggcattgt gggcgaatcc
    16561 ggttcaggaa aatcggtaag ttctctcgct attatgggct taatcgattt ccccggcagg
    16621 gttatggctg aaaaattgta ttttaacggc aatgatttat tggcattaaa accaaaagaa
    16681 aaacaacaga ttgtaggggc tgatgtctca atgatcttcc aagatgcaat gaccagctta
    16741 aatccgagct atactgtggg ctatcagatt atggaagccc taaaagtgca tcaaggtggc
    16801 tcaaaagcca gccgtcgtga gcgagcgatt gagttgctga cgatggtcgg tattcccgat
    16861 cctgaatccc gtttagaagt ttatccgcac cagctttcgg gtggtatgag ccagagagtg
    16921 atgattgcga tggcgattgc ctgtcgcccg aaattattga ttgccgatga gccgactact
    16981 gcgttagatg tgacgattca agctcaaatc attgatttat tacttgaatt acaacgcaaa
    17041 gaaaatatgg cgttgatttt aatcacgcac gatttagcgt tagttgccga gtctgcccat
    17101 cgcattatcg tgatgtatgc agggcaagtc gtggaagagg gcaaagccga gcagatattc
    17161 aaagcaccac tgcaccctta cacccaagca ttgctcaaag ccttaccgga atttgccgaa
    17221 gggaaatccc gcctgcaatc actaccgggc gtagtgccgg gcaaatatga ccgcccacaa
    17281 ggttgcttac tcaaccctcg ctgcccgtat gcaaccgaaa aatgccgaac cattgaaccg
    17341 gcaatgcaga taatcaacgg cagacaggtg aaatgccaca cgccactgaa tgcacagggt
    17401 gagccgataa ggatagctgt ataacgtcat aaagagtagg gacgcaatgc ttcgcgtccg
    17461 ttacaagcgg tgatttttaa ccgatttttg caaaataacg atagtggacg caaagcattg
    17521 cgtccctaca ttttccgaga aggaaaacag aatgaaagaa aataattctc tcccaccatt
    17581 cctcaaagca gtggacttaa aaaaatacta cccagttaaa aagggaatgt tcgctaaacc
    17641 gaaaacggta aaagcgctgg atggtgtctc ttttaccctt gaacgtggca aaacccttgc
    17701 gattgttggc gaatccggct gtggaaaatc aactttgggg cgaatgctca ctatgattga
    17761 aagcccaacc gaaggtgaac tgttctacaa cgggcagaat ttcttagtca atgacaaaga
    17821 aacggctcaa ctacgccgta aaaaaatcca aattgtgttt cagaacccgt atagctcgct
    17881 caatccacgc aaaaaagtag gtagcatttt agaagaacca ttattgatta acaccaactt
    17941 atccgcaaaa gaacgcaaag aaaaagtgtt ggaaatgatg gcgaaagtcg gcttaaaacc
    18001 ggaattttac gaccgttatc cgcatatgtt ttcgggcgga caacgccagc gaattgcgat
    18061 tgctcggggc ttaatgttgc agcctgatat tgtggttgcc gatgaacccg tttccgcatt
    18121 agatgtttcg gttcgggcgc aagtgttgaa tttgatgatg gatttgcaag aagaaatggg
    18181 cttgtcttac gttttcatct cgcacgattt atccgtagtg gaacatattg ccgatgaagt
    18241 gatggtgatg tatttagggc gttgcgtaga acaaggggat accaaaacta tcttcagcaa
    18301 tccacgtcac ccttatacac aagcgttact ctccgcaacc cctcgtttga acccggagca
    18361 acgccgtgag cggattaaac tgactggcga actaccaagc ccgcttaatc caccaaaagg
    18421 ctgtgccttt aacgcccgtt gcagatttgc aaccgatcgt tgctatgtgg agcgaccaga
    18481 gctgaaaacc tatcaagacg gtactcgcat tgcttgtttt gtggtggagg aaaataatta
    18541 ggcaacgctc gctatttaaa aggttaaagt ttttcaactt taacctttat ttttcatact
    18601 taatactcct catcttttgt agcaaaatct ttcattaatt tgctgatatg ctcacaatgg
    18661 tgtttaaatg acaatacggg caaataatca aatgcgtttt aacgccaatc ttctctttta
    18721 aagtgagttt tcgttccttt gagtcagaaa ttaaccttgt tacttgtcgg caattcatct
    18781 atttctctcc gttaatttcc ttgaaagaca ttgttgtaac tgcagccttg ct
//
LOCUS       DANLUQ010000037        18615 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.037, whole genome shotgun sequence.
ACCESSION   DANLUQ010000037 DANLUQ010000000
VERSION     DANLUQ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 18615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18615
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.037"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            89..1507
                     /gene="leuC"
                     /locus_tag="PXB27_001007"
     CDS             89..1507
                     /gene="leuC"
                     /locus_tag="PXB27_001007"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HDL5345815.1"
                     /translation="MQQAKTLYQKLFDSHVVYEAEGETPIIYINRHLIHEVTSPQAFD
                     GLRVAGRQVRQVSKTFGTMDHSISTQVRDVQQLEGQAKIQVLELEKNCEETGIPLFDI
                     TKKQQGIVHVMGPEQGLTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTL
                     KQARAKSMKVEVRGKVNPGITAKDIVLAIIGKTTMAGGTGYVVEFCGEAIRDLSMEGR
                     MTVCNMAIEFGAKAGIVAPDETTFAYLKDRPNAPKGQDWEDAIEYWKTLKADEGAVYD
                     AEVILEAKDIAPQVTWGTNPGQVIGIDDVIPNPEDMADPVTRASAEKALAYIGLEAHT
                     DMKDVPVDQVFIGSCTNARIEDLRAAAAVMKGRKKADNVKRILVVPGSGLVKEQAEKE
                     GLDKIFIEAGAEWRNPGCSMCLGMNDDRLGEWERCASTSNRNFEGRQGRNGRTHLVSP
                     AMAAAAAVFGKFMDIRKVSLNA"
     gene            1520..2122
                     /gene="leuD"
                     /locus_tag="PXB27_001008"
     CDS             1520..2122
                     /gene="leuD"
                     /locus_tag="PXB27_001008"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HDL5345816.1"
                     /translation="MAGIKQHSGLVVPLDAANVDTDAIIPKQFLQAITRVGFGKHLFH
                     EWRYLDAEETQINPDFVLNFPQYQGATILLARKNLGCGSSREHAPWALADYGFKVMIA
                     PSFADIFYNNSLNNHMLPIKLTEEEVEEIFQWVWANEGKQIHVDLEAMTVTVGEKVYT
                     FELDEFRRHCLLNGLDNIGLTLQHEAAIAAYEKNIPAFLR"
     gene            complement(2194..2658)
                     /locus_tag="PXB27_001009"
     CDS             complement(2194..2658)
                     /locus_tag="PXB27_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345817.1"
                     /translation="MNKFKIALMATVAIGLFACTPSPQSNSTKQQAQVGYLKQNISQA
                     ELNNPKDFKRYSYYCKNFATGSSSYLATYFPLWSESRKQENFGIYFQLDGGKVYPFDH
                     LANISLNARATKFEVRYRSYQPIEGSFVELVAREFTSTYYKNNVPWLECREG"
     gene            complement(2682..3188)
                     /locus_tag="PXB27_001010"
     CDS             complement(2682..3188)
                     /locus_tag="PXB27_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345818.1"
                     /translation="MADPHIQSPMDFWDYLTVIIYRSGFVLATLMLLLLPYYPQTAQY
                     GLLVSGTMLASSLHLYAKSFRLIFQFSVWIGLLGAVFNFPLLAFGAMLLVIGGLSYKE
                     YFCFRVFGLNFQPLLVAVLWIAFALEWSLLVQILSLVCGLLLLVLSIQKWRMPLHFDI
                     GDKSKYQI"
     gene            complement(3211..4188)
                     /gene="dusA"
                     /locus_tag="PXB27_001011"
     CDS             complement(3211..4188)
                     /gene="dusA"
                     /locus_tag="PXB27_001011"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HDL5345819.1"
                     /translation="MTNEIQPFYRGRFSVAPMLDWTTRHCRYFHRQFSRQALLYTEMI
                     TAPAIIHAKYDLLEYDPSENPVALQLGGSDPAQLAHCAKLVAERGYAEINLNVGCPSD
                     RVQNGMFGACLMAKADLVADCIKAMQDAVEIPVTVKHRIGIDDLDSYEFLCDFIEKVQ
                     PYSNDFIVHARKAWLTGLSPKENREIPPLDYDRVYQLKRDFPHLKISINGGIKTIEEI
                     KQHLQFVDGVMVGREAYQNPSMLGEIDSQIFNENRPLVTAKEAVEKMFPYIERELAKG
                     VYLNHIVRHMLGAFQHCKGARQWRRHLSENATKSGAGVEVVEQALAFIG"
     gene            4539..5516
                     /locus_tag="PXB27_001012"
     CDS             4539..5516
                     /locus_tag="PXB27_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HDL5345820.1"
                     /translation="MSRNLFQKRLNIKPYEYPELLEFKDAIRHSYWLHTEFNFTGDIQ
                     DYRTTINDHERHVLTRAMLAISQVEVNVKRFWGELYCYFPKPEMDDVGGTFAESEVRH
                     KDAYSFLLEKLGLNEMFSQIHEIEPLMNRIRYMESFMKDKDAGKGEFVLSLVLFSLFV
                     EHISLFGQFIIMMSFNKHKNLFKGISNAVEATSKEEEIHGKFGIALYEILRDEHSELF
                     TPEFFEDLQTLANQAFEAERGILDWIFEEGDLSFITKATVENYIKNRYNNSLSILGLE
                     PPHDIDPELLKETEWFDIEILSTKETDFFNKRSTDYSKKMKQITADDLF"
     gene            5503..7170
                     /locus_tag="PXB27_001013"
     CDS             5503..7170
                     /locus_tag="PXB27_001013"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HDL5345821.1"
                     /translation="MTYFNTDRPDFHWLNEDSRLFLQRGYLLEGTTALDRIRFIAEHA
                     ERKLGIEGYADKFYHYMARGYFSLSSPIWSNFGLDRGLPISCFGSYIGDSIHEIMVTT
                     AEVGMMSKIGGGTSAYFGDIRPRGSLIKNNGKSDGSFNFSKLFDTVIDVISQGTSRKG
                     QFAGYIDIEHGDIDEWLDIHTEGNPIQLMYYGVCVGHDWLESMKAGDPYKRQLWAKLL
                     QRKTETGIPYLFFKDNANAGRPDVYKDKNMTIHASNLCTEIMLPSSNEESFVCCLSSM
                     NLLYFDEWKDTDAPETLTYFLDVVMSEFIEKSQSIPFLDRANRFARRHRALGLGVLGW
                     HSYLQANNIAFDSFQAMQKNNEIFKTLQAKTLKASQELAQRFGEPEILEGYGRRNTTL
                     MSIAPTKSSSFILGSVSPSVEPFKSNYYVKDLAKIKTVYKNPFLEKLLQEKGLDTEEI
                     WTSILQNDGSVQHLEQLSEHEKEVFKTFAEISQLSVIQQAAQRQKYIDQGQSINVMIH
                     PATPARDLNQLYLTAEELGLKSIYYQNSMSAAQVFNRNLLSCSSCEG"
     gene            7174..7593
                     /locus_tag="PXB27_001014"
     CDS             7174..7593
                     /locus_tag="PXB27_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345822.1"
                     /translation="MGNLSHTSPNTIVEVVGEFPEITSSGYTFDKGPIQLRIGKHTAP
                     NKGFGVKHIEAEHMQEILNLGFTSVNEFVKAIIVENAKIFCEFSDLKGNHRPIILKAS
                     IGIVVLERKFLESGTIYSVVTAYRRTNPHGIQIGTMK"
     gene            complement(7828..8589)
                     /gene="nudC"
                     /locus_tag="PXB27_001015"
     CDS             complement(7828..8589)
                     /gene="nudC"
                     /locus_tag="PXB27_001015"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HDL5345823.1"
                     /translation="MNKGYWIIVSDFDIALEAGEIPFGSAEELGLSGREKLTIGQYQG
                     SPAYLVKALESDNQREFAYLRSQINRPPELSHLLHRAVSLNHFFQTHQFCGKCGEKTI
                     LASDEIAIYCNACGNRAYPTISPSIIVAVRKGRQILLANHLRHKGTIYTTLAGFVEAG
                     EAVETAVHREVFEETGIKVKNVRYFGSQPWAFPNSLMLGFLADYESGEIQLQEEEICD
                     AKWFDCEESLPEIPQQGTIAFELIQATLKLCRESK"
     gene            complement(8582..9265)
                     /locus_tag="PXB27_001016"
     CDS             complement(8582..9265)
                     /locus_tag="PXB27_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HDL5345824.1"
                     /translation="MKQPTFKRMTNKRSNFAKNLQKNPQKRPLTFAETTLVLLNKPYD
                     VLTQFTDEQGRKTLKDFITMPNIYPVGRLDRDSEGLLLLTNNGEIQHRLAHPKFDKQK
                     TYWVQVEGVPQPEDLAKLAKGVELKDGLTKPAKVRAIATPDFAWNIAPQIRERKTIPT
                     SWIELIISEGKNRQVRRMTAHIGFPTLRLIRVGIGQFRLGNLGCGEFQVLSENEKRKL
                     FTQLGLKDE"
     gene            complement(9262..10110)
                     /locus_tag="PXB27_001017"
     CDS             complement(9262..10110)
                     /locus_tag="PXB27_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL5345825.1"
                     /translation="MEKRTLALLKVHGTAVLFGASGIFGALIQSSAETIVLGRVMIAF
                     AIISLYFLWKKQPLVKLNLQQMAQLAFSAALLAAHWVTFFVAVKVGGVAVATLGFASF
                     PAFVALFESLFFREKLRFREVVLLIAITVGLILVTPEFTFGNQATQGLLWGVLSALVY
                     GILAVANRRTMSKISGVQASWWQYIFATLFLLPLSITEFPSVSTQDWFWIFCIGFLCT
                     TLAYTLFISSLDTINARTASMIISLEPVYAIVIAWLCFNEVPTLKMIIGGLIILLSVA
                     WANLKK"
     gene            complement(10110..10595)
                     /locus_tag="PXB27_001018"
     CDS             complement(10110..10595)
                     /locus_tag="PXB27_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HDL5345826.1"
                     /translation="MPILAILFGIFFYIYCEISLLVNIGSIIGVLPTILLLVVISFLG
                     LWIVKLRGVYTLFSIKQDLSQGKIPTEAVGNSVMFIVAGILLIIPGFLSDILAILCVL
                     PFSRKWIQAFVINSVKNKVFMHFTAQTSSFSVTSAQDSNTFDAEFERKPDEQDQHNRI
                     K"
     gene            complement(10597..11919)
                     /gene="pepQ"
                     /locus_tag="PXB27_001019"
     CDS             complement(10597..11919)
                     /gene="pepQ"
                     /locus_tag="PXB27_001019"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HDL5345827.1"
                     /translation="MKNLFSTHLARLQQTVQSLLENAFLDGLWIYAGKAKTLFLDDQT
                     APFKINPYFNYFFPYPQAENCWLFLDGKNKPKIYFYAPEDYWVSTLKVPTEAFFAAEF
                     EWVLLRAENEIATFIQNPTACAFIGEDVELAKKLGFSHINPQKVLNSLNFARSIKTEF
                     EIESIYQAQFSALKGHQVAKEAFFAGKSEFEINLEYLKATQQSDLNVPYGNIVAINQN
                     GAVLHYTALDFDTHAKRHSFLIDAGTTVNGYASDLTRTYAFDSGSEFAELVAKMEQLK
                     LQIIAELSIGVNYLHYHTRMQQMISQLLAEHQFIHLSAEQIFEQGISRTFFPHGLGHL
                     LGIQVHDVGGFQQNARGSHKAPPEVYPSLRCTRELQENMVLTIEPGFYFIEMLLKPWK
                     NSALSSAFNWQKIEAFKAFGGIRTEDNIVMRANGAENLTANAAATLGG"
     gene            11930..12172
                     /gene="tusA"
                     /locus_tag="PXB27_001020"
     CDS             11930..12172
                     /gene="tusA"
                     /locus_tag="PXB27_001020"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005710491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HDL5345828.1"
                     /translation="MTQLTVNKELDTLGLRCPEPVMLTRKTIRNMEEGEILHIIADDP
                     ATTRDIPSFCEFMDHQLIQKEVDSTPYHYWIKKGLE"
     gene            complement(12239..12670)
                     /gene="rraB"
                     /locus_tag="PXB27_001021"
     CDS             complement(12239..12670)
                     /gene="rraB"
                     /locus_tag="PXB27_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HDL5345829.1"
                     /translation="MYNLDELRTETDETISSLLADGSDPEALYIIEHHVAHHDFDKLE
                     KLVVDAYKLGYEISEAEEVEDENGKVIFVCDIVSEIHLNADLITAQQKELLPLIEKAG
                     AEYEGWGTYFEDPNADEDEYGDDGEFFDDENDDFEDEPRRH"
     gene            complement(12685..13296)
                     /locus_tag="PXB27_001022"
     CDS             complement(12685..13296)
                     /locus_tag="PXB27_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDL5345830.1"
                     /translation="MKLNYHKTHFLTSAPDIRHLPEDTGVEIAFAGRSNAGKSTALNA
                     LTNQKSLARTSKTPGRTQLINLFKVETNCKLVDLPGYGYAAVPEQMKIQWQKALGEYL
                     QKRECLGGVVILMDIRHPLKDLDQQMIEWAVSANLPVLLLLTKADKLSQSARSKQVKM
                     VREAILPFQGDIQVEAYSALNKIGIDKLSNKLDSWFAPKLITE"
     gene            complement(13298..13714)
                     /locus_tag="PXB27_001023"
     CDS             complement(13298..13714)
                     /locus_tag="PXB27_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDL5345831.1"
                     /translation="MSWYKALFSFNGRLNRRGFWIGFGIHFLFLFMVAMWINLAELSL
                     LSVLPLIITGFSLSAIIIKRLHDRNRSAKALLMALVPIICYLAAPYSQGIMQILLGQV
                     MPAFIGMLLFLEWGVFAGNPEPNQYGEQGLSFKFRE"
     gene            complement(13718..14548)
                     /locus_tag="PXB27_001024"
     CDS             complement(13718..14548)
                     /locus_tag="PXB27_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HDL5345832.1"
                     /translation="MFEYFLSLSPIWQAFLAGLFTWGCTIFGSSFVYFFKSVNRKLLD
                     SMMGFAAGVMIAASFWSLLAPSLEYAEADYGSLAWLPAAIGFLAGGAFIRLIDYVVPH
                     LHLSKPIEQAEGNDQLKQGLSKSTLLFLAITIHNIPEGLAIGVAFGALATQVSGVDAS
                     IMGAIGLAIGIGLQNIPEGSSLVLPIRGEGHSRKKAFWYGSMSAVVEPIAAVIGAVFV
                     LSMTAILPYALAFAAGAMIFVVVEELIPESQSSGNTDIATLSLMLGFVVMMVLDVALG
                     "
     gene            complement(14885..15000)
                     /gene="rrf"
                     /locus_tag="PXB27_001025"
     rRNA            complement(14885..15000)
                     /gene="rrf"
                     /locus_tag="PXB27_001025"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(15162..18060)
                     /locus_tag="PXB27_001026"
     rRNA            complement(15162..18060)
                     /locus_tag="PXB27_001026"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(18377..18452)
                     /locus_tag="PXB27_001027"
     tRNA            complement(18377..18452)
                     /locus_tag="PXB27_001027"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18417..18419),aa:Ala,seq:tgc)
     gene            complement(18509..18585)
                     /locus_tag="PXB27_001028"
     tRNA            complement(18509..18585)
                     /locus_tag="PXB27_001028"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18549..18551),aa:Ile,seq:gat)
BASE COUNT         5479 a         4298 c         3617 g         5221 t
ORIGIN      
        1 tccatatcag caagcggtca tttttgcaaa attttttgca aatttaaccg cttgtattaa
       61 aagttaccct tttgagttgg aacattttat gcaacaagcc aaaaccctat atcaaaaact
      121 attcgactca cacgttgtgt atgaagccga aggcgaaacg ccgattattt atattaaccg
      181 ccacttaatt cacgaagtta ccagcccgca agcgtttgac ggcttgcgtg ttgccggtcg
      241 ccaagttcgc caagtcagta aaaccttcgg cacaatggac cacagtattt caacccaagt
      301 gcgtgatgta cagcaacttg aagggcaagc gaaaattcaa gtattagaat tagaaaaaaa
      361 ctgcgaagaa accggtatcc cactgtttga catcactaaa aaacaacaag gtatcgttca
      421 cgtgatgggg ccggagcaag gcttaacttt acccggtatg accattgtat gtggtgactc
      481 gcacaccgcc actcacgggg cctttggtgc gttagccttc ggtatcggta cttccgaagt
      541 ggaacacgtt ttagccaccc aaaccttaaa acaggcccgt gcaaaaagta tgaaagtgga
      601 agtccgtggc aaagtaaacc cgggcattac cgcaaaagat attgtgcttg cgatcatcgg
      661 taaaacaact atggctggcg gtacaggcta tgtagtggaa ttttgtggcg aagcaattcg
      721 tgatctttca atggaagggc gaatgacagt gtgtaatatg gcgattgagt ttggtgcgaa
      781 agccggtatt gttgccccgg atgaaaccac gttcgcttac ttgaaagacc gcccaaatgc
      841 accaaaaggt caagattggg aagatgccat tgaatattgg aaaaccctca aagccgatga
      901 gggtgcagtt tacgatgcgg aagtgatctt agaagcaaaa gacattgctc cacaagtgac
      961 ttggggaacc aacccggggc aggtgatcgg tattgatgat gtaattccaa atccggaaga
     1021 tatggccgat cctgttactc gtgcctctgc ggaaaaagcc ttagcttaca tcggcttaga
     1081 agcccataca gatatgaaag atgtaccggt tgatcaagtc tttattggtt cttgtactaa
     1141 tgctcgtatt gaagatttgc gtgctgcggc agcagtaatg aaagggcgta aaaaagccga
     1201 caatgtaaaa cgtattctcg ttgtgccagg ttcaggtttg gtaaaagagc aagcagaaaa
     1261 agagggcttg gataaaatct tcattgaagc cggtgcagaa tggcgtaacc cgggttgctc
     1321 aatgtgtttg ggaatgaacg atgaccgctt gggcgaatgg gaacgttgtg cctctacgtc
     1381 taaccgtaac tttgagggca gacaaggacg taacggtcgt acccacttag tcagcccggc
     1441 aatggcggct gcggcggcgg tgttcggtaa atttatggat attcgcaaag tgtcattaaa
     1501 cgcataatag gaggaaaaaa tggcaggtat taaacaacat tcaggcttgg tcgttcctct
     1561 tgatgcagcg aacgtagaca ccgatgcaat cattccaaaa caatttttgc aagccattac
     1621 tcgagtcggc tttggtaagc accttttcca cgaatggcgt tatttagatg cggaagagac
     1681 tcaaatcaac cccgatttcg tactgaactt cccacaatat caaggtgcaa ccattttact
     1741 cgcccgtaaa aatttaggct gtggctcttc tcgtgagcac gccccttggg cattggcaga
     1801 ttacggtttt aaagtaatga tcgccccaag ttttgcggat attttctaca ataacagctt
     1861 aaataaccat atgttgccga ttaaattaac cgaagaggaa gtcgaagaga tcttccaatg
     1921 ggtgtgggca aatgaaggaa aacagatcca cgttgattta gaagcgatga ctgtgactgt
     1981 tggcgagaaa gtttatacct ttgagttaga cgaattccgc cgccactgct tattaaacgg
     2041 tttggataat atcggcttaa ccctacaaca cgaagctgcg attgctgctt atgagaaaaa
     2101 cattccggca tttttacgct aattaaaata caaaaaaacg gctaacatct ttaaggcgtt
     2161 agtcgttttc tttttagcca ttttctttgg caattagcct tctcggcact ctaaccaagg
     2221 cacattattt ttgtaataag ttgaagtaaa ctctcgtgcc actaactcta caaaactgcc
     2281 ttcaatcggc tgataagaac gataacgcac ttcaaatttt gttgctcttg cattcaaact
     2341 gatgttggca agatggtcga acggataaac ctttccgcca tcaagttgaa aatagatacc
     2401 aaaattctct tgcttacggc tttcagacca aagtggaaaa taggttgcca aatatgaact
     2461 gctgcctgtt gcaaaattct tacaataata gctataacgc ttaaaatctt tcggattatt
     2521 caattccgct tggctgatat tctgtttcaa atatccaacc tgtgcttgtt gcttagttga
     2581 gtttgattgt ggtgagggcg tacaagcaaa taagccaatt gccactgttg ccattaaagc
     2641 aattttgaat ttattcataa aaatcccgat aaaatattaa attaaatctg atatttgctt
     2701 ttatcgccaa tatcaaaatg cagcggcatt cgccattttt gaatactcag cacaagaagc
     2761 aacaagccac agacaaggct taaaatttgc accaataaag accattctag tgcaaaagca
     2821 atccacaata ccgccactaa aagcggttgg aaatttaagc cgaatactct aaaacagaaa
     2881 tattctttat agcttaatcc gccgatcacc agtagcattg ccccgaacgc aagcaaaggg
     2941 aaattaaaca cagcaccaag taagccaatc caaacagaaa attggaaaat caggcgaaac
     3001 gatttggcat aaagatgcaa agaagatgcc aacattgtgc cagatactaa taaaccatat
     3061 tgtgccgttt gcggataata aggcaataaa agcaacatca atgtagctaa cacaaaaccg
     3121 ctgcggtaaa tgatgacggt taaataatcc caaaaatcca ttggcgattg aatatgtgga
     3181 tctgccataa ctgcctcctt aaacaataaa ttagccaata aacgcaaggg cttgttccac
     3241 tacttctaca ccggctcccg acttagtggc attttcactt aaatgacgac gccactgcct
     3301 cgctcctttg caatgttgaa acgcaccaag catatggcga acgatatgat ttaaatagac
     3361 acctttggca agctcacgtt caatataagg gaacatcttt tccaccgctt cttttgccgt
     3421 tacaagcggt cgattttcat taaaaatttg cgaatcgatc tcgcctagca ttgacgggtt
     3481 ttgataggcc tcacgcccta ccattacccc atcaacaaac tgcaaatgct gtttaatttc
     3541 ctcaatggtt ttaatgccgc cattgatgct aatctttaaa tgcggaaaat ctcgcttgag
     3601 ctgataaacc cgatcataat ctaaaggcgg aatttcacgg ttttccttcg ggcttaaccc
     3661 tgtcagccac gccttgcggg cgtgtacgat aaaatcattg ctataaggtt gcactttttc
     3721 gataaaatca cacaaaaatt catagctgtc taaatcatca atcccaatac gatgtttaac
     3781 cgtgaccgga atttccaccg catcttgcat tgctttaata caatccgcca ctaaatctgc
     3841 ttttgccatc aaacaagccc caaacatacc gttttgcact cggtctgacg ggcaaccgac
     3901 atttagatta atctcggcat agcctcgctc tgccaccaat ttggcacaat gagccaactg
     3961 tgccgggtcg cttccaccca gttgcaaagc caccggattc tcgctcggat catattccaa
     4021 caaatcatat ttggcgtgaa taatcgccgg ggcggtgatc atttcggtat aaagcaaggc
     4081 ttgacgacta aattggcgat gaaaatagcg gcagtgacgg gttgtccaat ctaacattgg
     4141 agcaaccgaa aaacgtccac ggtaaaaagg ttgaatttcg ttagtcataa aaatatcaaa
     4201 ataaagcggt aaaaaatcag taaaaaataa ccgcttgtaa gcgattcaaa acaagcagcc
     4261 atcatactcg aaaaagccag ataaacatag tttcctcaag gcttccccat ctatctgcgc
     4321 acttttaaaa caaccttaaa acacaatata tagtgtttca acatcaaatc acacacaaca
     4381 tattgaagca aaaatttaca aaattatttt tctttataaa tcaataagct accaaagacc
     4441 aataaaaaag agtgtttgtc aagacttgtc ttgaaacttt ttttgcatac aatcatctac
     4501 cagttccaaa cataaacttt aagatagagc ctacgaatat gtctagaaac ctcttccaaa
     4561 aacgcttaaa tattaagccg tatgagtatc ctgagcttct tgaatttaaa gatgcaattc
     4621 gccattctta ctggttacac accgaattta acttcaccgg cgatattcaa gactatcgca
     4681 ccaccattaa cgatcacgaa cgtcacgtgt taacccgagc aatgcttgcg atttcacaag
     4741 tagaagtgaa tgtaaaacgt ttctggggcg agctatactg ctatttccca aaaccggaga
     4801 tggacgatgt aggcggtact tttgctgaaa gtgaagttcg ccataaagat gcttactcgt
     4861 tcctattgga aaaattaggc ctaaatgaaa tgttcagtca aattcatgaa attgaaccat
     4921 taatgaaccg cattcgttat atggaaagtt ttatgaaaga taaagatgct ggcaaaggcg
     4981 aatttgtttt atcgttagtg ctgttctctt tatttgttga gcatatttca ttgtttggac
     5041 agtttattat catgatgtcg ttcaacaagc ataaaaacct gtttaaaggg atttccaatg
     5101 cggttgaagc cacctcaaaa gaggaagaaa ttcacggtaa attcggaatt gcgttatacg
     5161 aaattttacg tgacgaacac agtgaactat tcacaccaga attttttgaa gatttgcaaa
     5221 ccttggcaaa tcaagcgttt gaagcagaac gtggcatctt agactggatt ttcgaagaag
     5281 gtgatttaag ctttatcacc aaagcgacgg tagaaaatta cattaaaaac cgttacaaca
     5341 attctttaag cattttaggg ttagagccgc cacacgacat cgaccctgaa ttattaaaag
     5401 agactgagtg gtttgatatt gaaattcttt caaccaaaga aaccgatttc tttaacaaac
     5461 gtagcaccga ttattctaaa aaaatgaaac agattaccgc agatgactta ttttaataca
     5521 gaccgcccgg acttccactg gcttaatgaa gacagccgtt tattcttaca acgtggctat
     5581 ttattggaag gaacaaccgc attagaccgc attcgtttta ttgccgaaca cgcagagcga
     5641 aaactcggca ttgaaggcta tgccgataaa ttctatcact atatggcaag aggctacttc
     5701 tcgctttctt cgccaatttg gtcaaacttt ggcttagatc gtggcttacc gatttcttgt
     5761 ttcggtagct acatcggcga ctctatccat gaaattatgg tgactaccgc tgaagtgggc
     5821 atgatgagta aaattggcgg tggtacttcg gcctactttg gcgatattcg tccgcgtggc
     5881 tcgttaatta aaaacaacgg taaatcagac ggctcattta acttcagtaa attatttgac
     5941 actgtgattg acgtgatttc acaaggaaca tcacgcaaag gtcagtttgc cggttatatt
     6001 gatattgagc acggcgatat tgacgaatgg ctggatattc acaccgaagg caacccaatt
     6061 caattaatgt attacggtgt gtgtgtcggt cacgattggt tagagtcaat gaaagccggc
     6121 gatccataca aacgtcagct ttgggcaaaa ttgttacaac gtaaaaccga aaccggtatc
     6181 ccttatttat tctttaaaga caatgccaac gcaggtcgtc ctgatgtata taaagataaa
     6241 aatatgacga ttcacgcctc taacttatgt actgaaatta tgttgccttc cagcaacgaa
     6301 gaaagtttcg tatgctgttt atcgtctatg aacctactct actttgatga gtggaaagat
     6361 accgatgcac cagaaacatt aacctatttc ctcgatgttg taatgagtga atttattgag
     6421 aaaagtcaat caattccatt cttagaccgt gcaaaccgct ttgcccgtcg ccatcgtgca
     6481 ttaggtttag gcgtgttggg ctggcacagc tatctacagg caaataatat tgcctttgac
     6541 agcttccaag cgatgcaaaa aaacaacgag attttcaaaa cgcttcaagc aaaaaccttg
     6601 aaagcctcgc aagaattggc tcaacgcttc ggcgagccgg aaattttaga gggctatggt
     6661 cgccgtaata ctacattaat gagtattgct ccgactaaat ccagtagctt tattttaggc
     6721 agtgtgtcgc catcggtaga gccgtttaaa tccaactact atgtaaaaga tttagcgaag
     6781 atcaaaaccg tgtataaaaa cccgttctta gagaaattac tccaagaaaa agggctagac
     6841 accgaagaaa tttggacgtc tattttacaa aatgatggct cggttcagca tttggaacag
     6901 ctttccgagc acgaaaaaga agtatttaaa acctttgccg aaatcagcca attaagcgtg
     6961 attcagcaag ctgctcaacg tcaaaaatat attgaccaag ggcaaagtat taatgtgatg
     7021 atccacccag ccacaccggc tcgtgatctt aaccaattat acttaacagc agaagagctg
     7081 ggcttaaaat ccatctacta ccaaaactca atgagtgcag cacaggtatt taaccgtaac
     7141 ctattaagct gctcaagttg cgaagggtaa ataatgggaa atttatcgca tacttcacct
     7201 aatactattg ttgaagttgt tggtgaattt cctgaaataa catctagtgg gtataccttt
     7261 gataaaggtc ctattcaatt aagaatcggc aaacatactg ctccaaataa aggctttggt
     7321 gtaaagcata ttgaagccga gcatatgcaa gaaattctta atctaggatt tacatcagtt
     7381 aatgaatttg tcaaagctat catagtagaa aacgctaaaa ttttttgcga attctcagat
     7441 ttaaaaggca atcatcgccc tattattctg aaagcaagta ttggcattgt ggttctagag
     7501 cgtaagtttt tggaaagcgg tacgatatat tcagttgtaa ccgcttatag aagaacaaat
     7561 cctcacggaa tacaaatagg cactatgaaa taaagtacgt tagtgttata atcagttcca
     7621 cgcaagagca tttgtagaaa ataacaggcg gatttggttt tctactgtgg gcggcaggtt
     7681 tccgctaacc tgtaatagct atttaatttt tatattaaat cctgaacttg aaacagacta
     7741 agggattgct gcggtagcta agaatacaaa cctctctata atcatataga gaggtttttt
     7801 cttattgatg agcacaagtt aagataatta tttactctcc cgacataact tcaaggtagc
     7861 ttgaatcagc tcaaaagcta ttgtgccttg ttgtgggatt tcaggtaagc tttcctcaca
     7921 gtcgaaccat tttgcatcgc agatttcttc ttcctgtagc tgaatttcac cgctttcata
     7981 atccgctaaa aaacctagca ttaatgaatt gggaaacgcc caaggttggc ttccaaaata
     8041 acgcacattt ttcactttaa tgcccgtttc ttcaaacact tcacgatgca ccgcagtttc
     8101 taccgcctca ccggcttcga caaaaccggc taaagtggta taaattgtgc ctttatggcg
     8161 tagatgattg gctagtagga tttgtctgcc ttttcgcacc gccacaataa ttgacgggct
     8221 aatcgttggg taagctcgat tgccacacgc attgcagtaa attgcaattt catcgcttgc
     8281 cagtatggtt ttctcgccac attttccgca aaattggtga gtctgaaaga aatggttaag
     8341 cgaaacagct cgatgcaaca aatgagacag ttcgggtggt cggttaattt ggcttcttaa
     8401 ataagcaaac tctcgctgat tatccgattc taacgccttc accaaataag ccggcgagcc
     8461 ttgatattgc ccgatggtta atttctcacg tccacttaag cccagctctt ccgcacttcc
     8521 gaatggaatt tccccagcct ccaaggcaat atcaaaatcg ctgacgataa tccaatatcc
     8581 tttattcatc tttcaatcct aattgtgtaa acaacttacg tttttcattt tcagatagca
     8641 cttgaaactc accacagcct aaattgccta atctaaactg gcctatccca acacgaatta
     8701 accgtaaagt cgggaaaccg atatgtgcag tcattcttct gacttggcga tttttccctt
     8761 cagaaataat caattctatc cagctggtcg gaatggtttt ccgctcacga atttgcgggg
     8821 cgatattcca agcaaaatcg ggcgttgcta ttgctctgac tttcgcgggc ttggtcaaac
     8881 catcttttaa ctccacccct tttgctaatt tggcaagatc ttccggttgt ggaacaccct
     8941 ccacttgtac ccaataggtt ttctgcttgt caaatttcgg gtgagctaaa cggtgctgga
     9001 tttcgccatt attagtcaaa agcaacaatc cttcactgtc tctatctagc cgcccgaccg
     9061 ggtaaatatt gggcatcgta ataaaatctt ttagcgtttt tcgcccctgt tcatcggtaa
     9121 attgggttag tacatcataa ggcttattga gtaacacaag ggtggtttcc gcaaaggtaa
     9181 gcggtcgttt ttgcggattt ttttgcaaat ttttagcgaa atttgaccgc ttgtttgtca
     9241 ttcttttaaa ggttggttgt ttcatttttt caggttcgcc cacgcaacag aaagtaaaat
     9301 aattaagcca ccgataatca ttttaagggt tggcacttca ttaaagcaaa gccacgcgat
     9361 cacaatcgca tacaccggct cgagggaaat aatcattgag gcagtacggg cgttgatggt
     9421 atcaaggctt gaaataaaca gcgtataggc aagggttgta cataaaaaac cgatacagaa
     9481 aatccaaaac caatcttggg tggaaacgct tggaaattca gtgatggaga gaggcaacag
     9541 aaaaagtgta gcaaaaatat actgccacca gcttgcttgc acaccggaaa ttttgctcat
     9601 ggtacggcga ttcgctaccg ccaaaatacc atacaccaaa gcagataaca cgccccacaa
     9661 taaaccttga gtggcttgat taccaaaagt aaattcaggg gtaactaaaa ttaaaccaac
     9721 cgtgatggca attaatagca ccacttcacg aaagcgtaat ttttcacgga agaacaggct
     9781 ttcaaataac gccacaaatg ccggaaagct ggcaaagcct aaggttgcta ccgccacacc
     9841 acctacttta accgccacaa aaaaagtaac ccagtgggca gctaataaag ctgcagaaaa
     9901 tgcaagttga gccatttgct gtaagttgag ctttacaagc ggttgttttt tccataaaaa
     9961 atacaaagag atgatagcaa aagcaatcat cactcgccca agcacaatgg tttcagccga
    10021 gctttgaatt aatgctccga agatccccga ggcaccaaat aaaaccgctg tgccgtgtac
    10081 tttaagtagt gcaagtgttc ttttctccac tatttgatcc tattatgttg atcttgctcg
    10141 tccggcttgc gttcaaattc tgcatcaaac gtgttgctat cttgcgcaga tgtaacagaa
    10201 aaagacgaag tttgggcggt aaaatgcata aacaccttat tctttacgct attaattacg
    10261 aaggcttgaa tccattttcg gctaaagggt aacacgcata aaatcgctaa aatatcactt
    10321 aaaaagccgg gaataatcag caaaatgccg gctacaataa acatcaccga gttgccgact
    10381 gcttcagtcg gtatcttgcc ttggcttaaa tcttgcttga tggaaaagag ggtataaacg
    10441 ccccttaact tcacaatcca taaccctaaa aatgaaatca cgaccagtaa caaaatcgtg
    10501 ggtaacacgc caatgatcga gccaatattc accagcagcg aaatttcgca atagatataa
    10561 aagaaaatac caaacagaat cgctaaaata ggcatattat cctcccaacg ttgcggcagc
    10621 gtttgccgtc agattttctg ccccattggc tcgcatgaca atattgtctt cggtacgaat
    10681 cccgccaaag gctttgaaag cctcaatttt ctgccagtta aaggcagaag ataaggcact
    10741 gtttttccaa ggcttaagta gcatctcaat aaaataaaaa cccggctcaa tggtaagcac
    10801 catattttcc tgtagctcac gggtacaacg caaacttggg taaacctctg gaggagcttt
    10861 atggctgcct cgggcatttt gctgaaaacc gcccacatcg tgcacttgaa tacccaataa
    10921 atgccctagt ccgtgtggga agaacgtacg gctaatccct tgttcaaaaa tttgctcggc
    10981 tgaaagatga ataaattgat gttccgctaa taattgcgaa atcatctgct gcattcgggt
    11041 gtgataatgc aggtagttca ccccaatact cagctcagca ataatctgca acttcaactg
    11101 ttccattttc gccaccagct cggcaaattc agaaccgctg tcaaaggcat aggtgcgggt
    11161 gagatcggag gcataaccat ttaccgttgt gccggcatca attaaaaaac tgtggcgttt
    11221 tgcgtgggtg tcaaaatcta acgccgtata atgtagcacc gcaccatttt gattaatcgc
    11281 cacaatattg ccataaggaa cattgagatc agattgctgc gtcgctttca aatattcaag
    11341 gttgatttca aattcgcttt tgccggcaaa aaatgcctct tttgctactt gatgcccttt
    11401 taaggctgaa aattgggctt ggtaaatact ttcaatttca aactccgttt tgatggagcg
    11461 ggcaaaattc aggctattca gcactttctg cggattaata tggctaaagc ctaacttttt
    11521 ggctagctca acatcttcac cgataaaggc acaagcggtc ggattttgga taaaggttgc
    11581 aatttcattc tctgctctca acaaaaccca ctcaaattcc gcagcgaaaa aggcctccgt
    11641 tggcactttt agagtggaaa cccaataatc ttcaggggca taaaaataga ttttcggctt
    11701 atttttgcca tctaaaaata accagcaatt ttcagcttgg ggatatggga aaaagtaatt
    11761 aaaataaggg ttaattttaa acggggcggt ttgatcgtct aaaaataagg tttttgcctt
    11821 gccggcataa atccataaac cgtctaaaaa ggcattttcc agcaagcttt gcactgtctg
    11881 ttgcaaacgt gcgagatgcg tagagaatag atttttcata aggaataaaa tgactcaatt
    11941 aactgtaaat aaagaattag ataccttggg gctacgttgc ccagagccgg tgatgctcac
    12001 ccgtaaaaca atccgtaata tggaagaggg agaaattttg catatcatcg ccgatgaccc
    12061 ggccactacc cgtgatattc cgagtttctg cgaatttatg gatcaccaat taattcaaaa
    12121 agaggtggac tccaccccat accattactg gattaaaaaa ggcttggaat aaaccacaag
    12181 cggtcgattt tagccataaa attgcaaatt tatgcacaaa atcgaccgct tgttttaatt
    12241 aatgacggcg aggttcgtcc tcaaaatcgt cattttcatc atcgaaaaat tcaccgtcat
    12301 cgccgtactc atcttcatca gcattcggat cttcaaagta ggttccccag ccctcatact
    12361 ccgcaccggc tttttcaatt aatggcagta actctttttg ttgtgcggta atgagatccg
    12421 cattcaagtg aatttcgctc acaatgtcgc atacaaaaat cactttacca ttttcgtctt
    12481 ccacttcttc cgcttcggaa atttcatagc ccagcttata ggcatcaact actaattttt
    12541 ccaatttatc aaaatcgtgg tgagcaacgt ggtgttcaat aatataaagg gcttcagggt
    12601 ctgaaccatc ggctaataat gatgaaatgg tttcatcggt ttctgtgcgt aattcatcga
    12661 gattgtacat attgaattcc ttacttattc tgtgattaat tttggtgcaa accagctatc
    12721 taatttgttc gacagcttat caataccgat tttattcagt gcggaataag cctctacttg
    12781 gatatcacct tggaatggta aaatcgcttc tcttaccatt tttacctgct tagagcgtgc
    12841 cgattggctt aacttatccg ctttggtcaa cagcagtaac accggcaagt tggctgacac
    12901 tgcccattca atcatctgtt gatctaaatc tttcaacggg tggcgaatat ccattaaaat
    12961 caccacaccg cctaaacatt cacgcttttg taaatactcg cctaatgctt tttgccactg
    13021 gattttcatc tgttcaggta ctgctgcata gccatagcca ggtaaatcca ctaatttaca
    13081 attcgtttca accttaaaca agttaatcaa ctgcgttcgc cccggtgttt tggaagttcg
    13141 ggctaaactt ttttggttag ttaaagcgtt caaagcagtc gatttaccgg catttgaacg
    13201 cccggcaaaa gcaatctcca cacctgtatc ttcaggtaaa tgacgaatat ccggtgctga
    13261 agttaaaaaa tgggttttat gatagtttaa tttcatttta ttctctaaat ttaaaagata
    13321 agccttgctc gccatattga ttcggttcag gattaccggc aaatacaccc cattctaaaa
    13381 agagcaacat tccaataaat gccggcatca cctgtccaag caatatctgc ataattcctt
    13441 gtgaataagg agctgcaaga taacaaataa tcggcactaa tgccattaac agggcttttg
    13501 ccgaacgatt tcggtcgtgt aatcgtttaa taataatagc agacaggcta aatcctgtaa
    13561 ttataagcgg tagcacactc aataaagaca attcagccaa atttatccac atagcaacca
    13621 taaacaggaa aagaaagtgg attccaaatc caatccaaaa acctcgtcta tttagcctac
    13681 cgttaaaact aaaaagtgct ttataccaac tcattttcta ccctaaagct acatcaagta
    13741 ccatcattac tacaaagcct aacatcaggc ttaaagtcgc aatatcagta tttcccgaac
    13801 tttgcgattc cggaatcagt tcttctacga ccacaaaaat catcgctccg gcagcaaagg
    13861 caagagcata aggtaaaata gccgtcatcg acagcacaaa aaccgcccca atcaccgccg
    13921 caataggctc aaccactgct gacatagagc cataccaaaa tgctttcttc cgactatgcc
    13981 cttcgcctcg aattggcaat acaagtgacg agccttcggg aatgttttgt aaccctattc
    14041 cgattgccaa accgatagct cccataatgg aagcatctac gcctgaaact tgggttgcca
    14101 aagcaccgaa agccacccct atggctaatc cttctggaat attatgaatg gtaatcgcaa
    14161 gaaaaagcaa agtagatttt gataagcctt gtttaagttg atcatttcct tcagcttgct
    14221 caatcggctt actcaagtgc aaatgcggca caacatagtc aattaatcga ataaaagctc
    14281 caccggcaag aaagccaatc gctgcaggca gccaagctaa agagccataa tccgcctcgg
    14341 catattctaa agaaggtgca agcaaagacc aaaacgaggc tgcaatcatt acacctgcgg
    14401 caaagcccat catactgtct aataattttc gattaaccga tttaaaaaaa tagacaaatg
    14461 acgaaccgaa aatcgtacaa ccccaagtaa ataaacccgc taaaaaagct tgccatattg
    14521 gagaaagtga aaggaaatat tcaaacaaga aatagtacca gctaaaataa atattggctc
    14581 attctatcaa agattcaggg ttattaatat agtgtagcca aaggctcaac gaagatttca
    14641 cttgtaaaac ttagcaagaa aataaccgct tgcatttccc tttcataaat acaatgaaaa
    14701 tcctaacaag cggtcatttt ccgtgagttt tttaccttta ccacaacaac aaaaaaccgc
    14761 ctgtatctct acaggcggct ctttcttgga ttacttgtac tttgtttatt tgtacttatt
    14821 ttgcttttgc ttaagttttc gcttttgtaa cttttagctt tcgcttaagc tcgcttaaat
    14881 taaaacccga cgatgctcta ctctcacatg gcgaatcacc acactaccat cgacgttact
    14941 gcgttttact tctgagttcg gtatggggtc aggtaggacc acagcactct tgtcgtcggg
    15001 aaaattcttt tctatttttg aatttcttta agttaacttc ctaatctaac tcttagttca
    15061 ctttaatagt tagattaact tccttaactt cgattgattt cttctctagt ttcgttcttc
    15121 ttctcaatac aaaaacaagc ctaaaaacat ttgagcgttg tatagttaag cctctcgggc
    15181 aattagtact ggttagctca atgtctcaca acacttacac acccagccta tctacgtcgt
    15241 agtctccaac aacccttaca cacttatagt gtgggagaac tcatctcgag gcaagtttcg
    15301 tgcttagatg ctttcagcac ttatctcttc cgcatgtagc tacccagcaa tgcctctggc
    15361 gagacaactg gaacaccagt gatgcgtcca ctccggtcct ctcgtactag gagcagcccc
    15421 tctcaattct ccaacgccca cggcagatag ggaccgaact gtctcacgac gttctaaacc
    15481 cagctcgcgt accactttaa atggcgaaca gccataccct tgggacctac ttcagcccca
    15541 ggatgtgatg agccgacatc gaggtgccaa acaccgccgt cgatatgaac tcttgggcgg
    15601 tatcagcctg ttatccccgg agtacctttt atccgttgag cgatggccct tccattcaga
    15661 accaccggat cactatgacc tgctttcgca cctgctcgac ttgtctgtct cgcagttaag
    15721 cttgcttata ccattgcact aacctcacga tgtccgaccg tgattagcaa accttcgtgc
    15781 tcctccgtta ctctttggga ggagaccgcc ccagtcaaac tacccaccag acactgtccg
    15841 agaccgcgtt ccgcaatctt cgttagaaca tcaaacgtta aagggtggta tttcaaggac
    15901 gcctccacaa tcactggcgt gactgcttca aaggctccca cctatcctac acatcaaaat
    15961 tcaatgttca gtgtcaagct atagtaaagg ttcacggggt ctttccgtct agccgcgggt
    16021 acaccgcatc ttcacggcga tttcaatttc actgagtctc gggtggagac agcctggcca
    16081 tcattatgcc attcgtgcag gtcggaactt acccgacaag gaatttcgct accttaggac
    16141 cgttatagtt acggccgccg tttactgggg cttcgatcag gagcttctct tgcgataaca
    16201 ccatcaatta accttccagc accgggcagg catcacaccc tatacgtcca ctttcgtgtt
    16261 tgcagagtgc tgtgttttta ataaacagtt gcagccagct ggtatcttcg accggttcaa
    16321 ccttcatccg caagggacta caatctacgc cggcgcacct tctcccgaag ttacggtgct
    16381 attttgccta gttccttcac ccgagttctc tcaagcgcct gagtattctc tacctgacca
    16441 cctgtgtcgg tttatagtac ggtttataat aacctgaagc ttagtggctt ttcctggaag
    16501 cgtggtatcg attacttcat ctccgtagag actcgtcatc acttctcggt gttaaggaag
    16561 accggatttg cctaatcttc ccacctaccg gcttaaacag acatccaaca gtctgctaac
    16621 ctaaccttct ccgtccccac atcgcagtta ttacaagtac gggaatatta acccgtttcc
    16681 catcgactac gcttttcagc ctcgccttag gggccgactc accctgcccc gattaacgtt
    16741 ggacaggaac ccttggtctt ccggcgaacg agtttttcac tcgttttatc gttacttatg
    16801 tcagcattcg cacttgtgat atctccagca gacttctcaa tccaccttca tcgacttaca
    16861 caacgctccc ctacccaaca gactttcgtc tgatgccgca gcttcggtgc tatatttgag
    16921 ccccgttaca tcttccgcgc aggccgactc gactagtgag ctattacgct ttctttaaat
    16981 ggtggctgct tctaagccaa catcctagct gtctaagcct tcccacttcg tttcccactt
    17041 aatatagact ttgggacctt agctggcggt ctgggttgtt tccctctcca cgatggacgt
    17101 tagcacccac cgtgtgtctc ctgagtatca ctcttcggta ttcgcagttt gcatcgggtt
    17161 ggtaatccgg gatggacccc tagccgaaac agtgctctac ccccgaaggt gtccgctcaa
    17221 ggctctacct aaatagattt cggggagaac cagctatctc ccggtttgat tggcctttca
    17281 cccccagcca caagtcatcc gctaattttt caacattagt cggttcggtc ctccagttag
    17341 tgttacccaa ccttcaacct gcccatggct agatcaccgg gtttcgggtc tataccttgc
    17401 aactacacgc ccagttaaga ctcggtttcc ctacggctcc cttattcagt taaccttgct
    17461 acaaaatata agtcgctgac ccattataca aaaggtacgc agtcacaaga ttactcttgc
    17521 tcccactgct tgtacgcaca aggtttcagg ttctatttca ctcccctcgc cggggttctt
    17581 ttcacctttc cttcacagta ctggttcact atcggtcaat caggagtatt tagccttgga
    17641 ggatggtccc cccatcttca aacaggatat cacgtgtccc gccctacttg ttgttagcct
    17701 agtaccacaa tggagtttta aggtacggga ttatcaccct ctacgattgt ccttcccaga
    17761 acattcccct aactttactg ctatcactaa ctggctcttt cgcgttcgct cgccgctact
    17821 aacgaaatct cggttgattt cttttcctcg gggtacttag atgtttcagt tctcccggtt
    17881 tgcctcacac agctatgtat tcactgggtg atagtaggtt cttcacctac tgggtttccc
    17941 cattcggaca tcttggatta aacgcctctt atcgactcat ccaagctttt cgcagattag
    18001 cacgtccttc ttcgcctctg attgccaagg catccacctt gtgcgcttag tcacttaact
    18061 atacaacctc aaatgttttc taaaacttct caaaaataaa agtaaaaaaa caatgaagtc
    18121 agttttacaa ctaaacactt gactgctttt gttcagtcaa gattttttct actcagactt
    18181 tcaatttatt cgttatcact tatgtataat tacttatgcg taacgaactt gaaatatctc
    18241 tcagtttttc agcttgtttc catattgtta aagaacaaag ataatggctt tcgcctatca
    18301 tcatacctaa aattgcaaat aatccggtat ttttaaccgc ttgtaatctt aggtatgatg
    18361 ataaagtcac gatgattggt ggagataagc gggatcgaac cgctgacctc ctgcgtgcaa
    18421 ggcaggcgct ctcccagctg agctatatcc ccatttcatc ttccgttttc ttcactcata
    18481 gtcgcttcgc tttcgcttgc cttctgagtg gtgggtctga gtggacttga accaccgacc
    18541 tcacccttat caggggtgcg ctctaaccac ctgagctaca gacccaacgg atgaccttcg
    18601 gttttttgtc ttctt
//
LOCUS       DANLUQ010000038        18147 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.038, whole genome shotgun sequence.
ACCESSION   DANLUQ010000038 DANLUQ010000000
VERSION     DANLUQ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 18147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18147
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.038"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(78..4064)
                     /gene="hrpA"
                     /locus_tag="PXB27_001029"
     CDS             complement(78..4064)
                     /gene="hrpA"
                     /locus_tag="PXB27_001029"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HDL5345833.1"
                     /translation="METPLQKNHKKPPLVKLENITTADFRRLLGRLKGAENIKNIDHK
                     RSVLAEISQDLTACQARFFARTQHAQNLKIDYPDLPVSARREEILKLISENQVVVIAG
                     ETGSGKTTQLPKMCLELGRGVKGLIGHTQPRRIAARSVATRIAEELKSELGETVGYKV
                     RFNDQVSENSLIKLMTDGILLAEIQHDSYLNQYDTLIIDEAHERSLNNDFILGYLKQI
                     LHKRPDLKVIITSATIDVERFSKHFNNAPIIEVSGRTFPVEVRYRPIVEDQDQLQGIL
                     NAVDELQAEGRGDILIFMNGEREIRDTAEALQKQELRHSEILPLYARLSAAEQQRIFQ
                     PSGGLNRIILATNVAETSLTIPNIKYVIDTGTARISRYSYRTKVQRLPIEPISQASAN
                     QRKGRCGRTSEGICIRLYSEEDFNSRPQFTDPEILRTNLAAVILQMTSLGLSDIANFP
                     FVDAPDSRQIQDGIRLLEELQAIIPNEKIPSTACGGGLGRGEIRSNDGTPPLPTSPRK
                     RGDGFRLTPIGRQLAQLPVDPRLGRMVIEAAKNGSLHEVMMIVSALSIQDPRERPQEK
                     QQSADDKHRRFADKESDFLAFVNLWHFIQAQQKELSKNQFRNLCRKDYLNYNRVREWQ
                     DIYHQLRLAVREMGLKINSEPASYQPIHTALLAGLLSHIGLKDNEKMHYLGARNAHFF
                     IFPNSALFKKQPKWVMAAELVETTKLWGRMVARIEPEWVEPLAVHLTKSSYSEPHWAK
                     SKGAVMASEKVSLYGIPIVAQRSVNYGSIDPTVSREIFIRSALVEGECHNNYKFFKEN
                     HRLIREVEALEHKSRRRDILVDEQVLFEFYDQRIGTEVVSSKHFDTWWKQASKKDPEL
                     LNFEKSFLINDNANLASELDFPNFWHQGDLKLKLSYQFEVGKDHDGVTVHIPLPLLNQ
                     VEPEGFDWQIPGLRHELIVSLIKSLPKSLRRNFVPAPNYADAFLGRAEPFKKPLLDNL
                     SHELRRMTGVLVEPEQWDLSQLPPHLRMTFRVIDDKGKKLQESENLDELKFALKDEVQ
                     NTLSNIADDGIEQSGVHIWNFAELPQFYEQKKAHFSVKAYPAIVDEQTAVGVKLFETE
                     FEQARAMQAGLRRLLLLNVPSPIKYLHEKLPNKAKLGLYFAPFGKVLELIDDCIACAV
                     DQLVEEFGGFVWTEEKFTQLHEFVRANLNDKTVEIAKQVEKCLTLAYELNKRLKGKLD
                     FTMAFALSDIKTQIEGLIYPDFVTKTGYARLADLYRYLTAIERRLDKLLVDSNTDRAK
                     MLRVEAVQEAYKQLLAKLPKSKAVPDEVLEIRYMIEELRVSLFAQQLGTKYPISDKRI
                     LNVINEIK"
     gene            complement(4168..4620)
                     /locus_tag="PXB27_001030"
     CDS             complement(4168..4620)
                     /locus_tag="PXB27_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HDL5345834.1"
                     /translation="MTLQFNAVALLLVVLIILGLISQNSAVTISAAVLLIMQQTLLSK
                     FIPFVDQYGLKIGIIILTIGVLSPLVSGRITLPELAQLLNWKMALSIIAGVLVAWLGG
                     RGVNLMGSQPVLVTGLLIGTVIGVAFLKGVPVGPLIAAGILSLIVGKT"
     gene            complement(4683..4952)
                     /locus_tag="PXB27_001031"
     CDS             complement(4683..4952)
                     /locus_tag="PXB27_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345835.1"
                     /translation="MDAAMSLRFDKLRFVKRLQEANQTPEMAEAFANALDEALEQSQS
                     PLATKADLQLELEKLKNEINTTIFKAITLNITILGFLMAIMKFIN"
     gene            complement(5050..5646)
                     /gene="grpE"
                     /locus_tag="PXB27_001032"
     CDS             complement(5050..5646)
                     /gene="grpE"
                     /locus_tag="PXB27_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005712850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDL5345836.1"
                     /translation="MTTQNEHLQEEVKQEEIQPEVVTEETQAEEQNTPQEEPLALAQA
                     RIAELEAYIAEADKREQDIQLRAQAEIQNIRRRAEQDVEKAHKFALEKFSKELLTVVD
                     NLERGLDALDKAVTDETTQALVDGVEMTHKEFINTLAKFGVVAIGEVGEAFNPELHEA
                     ISMQPAEGIEANHVSTVLQKGYTLHGRVIRPAMVMVAN"
     gene            5868..6962
                     /locus_tag="PXB27_001033"
     CDS             5868..6962
                     /locus_tag="PXB27_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL5345837.1"
                     /translation="MKKLAGLFAAGLATVALTACNEEKAAEPKATAAEAKPQATNQTV
                     HLYTWTEYVPEGLLDEFTKETGIKVEVSSLESNETMYAKLKLQGKDGGYDVIAPSNYF
                     VSKMAKEGMLAELDHAQLPVIKELNQDWLNKPYDQGNKYSLPQLLGAPGIAFNTADYK
                     GETFTSWGDLWKPEFAGKVQLLDDAREVFNIALLKLGKNPNTTNPDEIKEAFEELKKL
                     RPNVLSFTSDNPANSFIAGEVSVGQLWNGSVRIAKKENAPVDMVFPKEGPVLWVDTLA
                     IPANAKNKENAHKLINYLLSAKVAEKLTLAIGYPTSNVEALKVLPKEITEDTAIYPSA
                     EVLQKSQWQDDVGDAIELYEKYYQELKAAK"
     gene            7098..8489
                     /locus_tag="PXB27_001034"
     CDS             7098..8489
                     /locus_tag="PXB27_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDL5345838.1"
                     /translation="MNLRWQEYPENARKLFKLSLPIFISQLSASGMGLADIVMAGLVS
                     DDDVSAIAVSNSIYFPLFLFVSGLLNAITPTVSYLNGSSNRHLIANQVRQGYWIVFAM
                     SIPLMAIFLNSHLILDLMQTPAEFSIKSQQYLAVMAVGLIPALLAVSLRCMNDGLSNP
                     KPAMRITFFGLLLNIPLNYIFIFGKFGLPEMGAVGCGVATAIVNLIMFFMLLHYCYTN
                     SAQKDIRLFEKWIDAPSGQILLKLCKLGLPIAFAIFTEVMLFSTSSLFLSPLGSQVVA
                     SHQAALQTSSLFFMIPMSFGIATTIMIGQTLGQKNVEGAKILSYHALITATALAIIAA
                     IIIILLKNFIPFAFTNNPISIAITAHLLIFAAVYQIPDAIQAVANGILRGYKHTQPIL
                     YVTLFCYWGVSMPLGYILARTDWIVQPMAAQGFWLVFCIGLSLAGGLLLWQMRKIQAV
                     PKAQLIAKLERIK"
     gene            8548..12072
                     /gene="recB"
                     /locus_tag="PXB27_001035"
     CDS             8548..12072
                     /gene="recB"
                     /locus_tag="PXB27_001035"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HDL5345839.1"
                     /translation="MKNLNPIQLPLNKSVLIEASAGTGKTFTIANLYLRLLLGIGCNP
                     LTVEQILVVTFTKAATEELKNRIRKNIQQCADFLKDQADGLEVESTKSYRNNLDFLAQ
                     IYPLIPNIHEALLRLSIAEREIDTASVFTIHGFCQKMLVQFAFESGVRFDLDLQPNQS
                     DLLKKLSEEVWREQFYPQDLAITYAVAEQLGTPEYALNAVRRYLSTELPEPNASLNQD
                     IAEHLAQYQQFINEVKSYWKTHSDELVETIRHDLKHKITLHGGWYRATTFEAGVEVMN
                     QWANSQHNHFPAGLYLFTTEKIAAGTKKSCEPIDSPHLAKNQAFLTAYKERFENSLSA
                     MLQYQFLIALKAKLAEYKQSHPQRSFDDLLTMLNQALKNLHTGEALSQKIRAMYPFAM
                     IDEFQDTDLTQYEIFSQIFMAENAENGFIMIGDPKQSIYKFRGADIFTYLRAAKQADE
                     QFTLGKNWRSLPEVVQAVNNLFSFANDNSPFLYEDIGFHPVEADSQKGQLVSEQGHFV
                     GYITENSKLDDLAEMCAYQIHQQLEAMAQGKFGVEKNGEFQSFAAKDIAILVRKGSQA
                     KRIKKALAKRNIKSVYLAESASVFGSEIATELCWLLQAALNPYHYKALLSALGSTLWG
                     LTAAEIHRYKENEQLWDLKVNQFVEYSNIWKEQGVLPMLHKIYLEQGIIERLKGLENA
                     DRLITDLLHLTEILQETSQALESEAELLRWYEKQVNSPDFNDEQQLRLESEEELIKIV
                     TIHGSKGLQYPIVWLPFVSQKSEGDTQTALSLYRNSQGALDWCLGSEEASFSLQYEAY
                     AEDLRLLYVALTRAESQLNLVLPKEISGWSPLHYLLADGEIPTGKVPVSDYFSQKRIA
                     ANLVEVAGELPFSLWEGEQSSDDYQAKTFSGNIRVVGQITSFTSLYAQHERFSEGKSE
                     RVMDLAQDRDVHIETIEAGYSSETAAFSPFQFPHGIKVGTVLHHFFEHCQFNEQIDRE
                     AVAKVCEQLGLSEEWIEPTALWFERILTTPLAEANFCLKAIDETKRLNEWQFYLRLKN
                     DKALHQLNALLKQHSPLAKTLPELQLPQLEGFVRGFVDCIVQVEEKFYLIDYKSNFLG
                     YLPQNYAKEHIQREMGRQRYDLQYLLYTLALHRYLTARLGEKYDYERDFGGVAYLFLR
                     AMNGENDNGVYFDKPTKQLIEEMDRLFG"
     gene            complement(12114..12596)
                     /gene="greB"
                     /locus_tag="PXB27_001036"
     CDS             complement(12114..12596)
                     /gene="greB"
                     /locus_tag="PXB27_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HDL5345840.1"
                     /translation="MAKSNYITRSGWQALDQELKFLWREERPKVTQAVSDAAALGDRS
                     ENAEYIYGKRRLREIDRRVRFLSKRLEVLQIVDYHPKQEGKVFFGAWVELENEEGEAK
                     QYRIVGCDEFDPAKNWISIDSPVARALIGKQVDDEIRVETPLGKVLLYINKIWYEQAS
                     "
     gene            complement(12647..13927)
                     /gene="pepB"
                     /locus_tag="PXB27_001037"
     CDS             complement(12647..13927)
                     /gene="pepB"
                     /locus_tag="PXB27_001037"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HDL5345841.1"
                     /translation="MTAMKISLSTQNAPEIWGKNAILSANDAGFTVHNPNNDLATIQK
                     AARKIKNQGISTVELTGDSWDLESCWAFHQGFHSVKNQGSVQYPDLDEQQAEFDNRVK
                     CATFVRELINKPSDKLTPEKLAEQAVEFIQKMAKPNTVSYQIIRGEALQEQGYHGIWT
                     VGKGSANPPAFLVLDFNPTNDENAQVLACLVGKGITFDTGGYSLKPSDSMSTMRTDMG
                     GAALLTGSLAFAIARGLNKRVKLYLCCAENMVSHNAFKLGDIITYRNGVTVEVLNTDA
                     EGRLVLADGLIDACKQNPQFIIDAATLTGAAKVAVGNDYHSVLSMDDNLTEALFKSAK
                     TENEPFWRLPFEEFHRGQIASSFADIANIGSVPVGAGASTATAFLSYFVENYQQNWLH
                     IDASATFRKTASDLWAAGATGLGIRTVANLLLSK"
     gene            complement(14066..14887)
                     /locus_tag="PXB27_001038"
     CDS             complement(14066..14887)
                     /locus_tag="PXB27_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010869033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HDL5345842.1"
                     /translation="MKNIKLSAMSLTALFVLTACSHTHKNSDEHHQMVLQEQATLGIN
                     WMQQSGEYQALAHQAFNAAKVAFDQAKVKKGKKKAVVVDLDETMVDNSLYAGWQVKNH
                     KPFDGESWTRWVNARQTGAIAGAVEFNNYVNSHKGTMFYVSNRKDSSEKAGTIDDMNK
                     LGFSGVSEQTLLLKKDKSNKTPRFEEIEKQGYEIVLYIGDNLNDFGDATYRKSNAERR
                     AFVAENSQQFGKKFIMLPNPNYGDWEGGLDKDYYKGNAESRVKIRHNAIKAWDGK"
     gene            15035..15978
                     /locus_tag="PXB27_001039"
                     /pseudo
     CDS             15035..15978
                     /locus_tag="PXB27_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
     gene            complement(16091..17254)
                     /locus_tag="PXB27_001040"
     CDS             complement(16091..17254)
                     /locus_tag="PXB27_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDL5345843.1"
                     /translation="MKRKTLATLVLIAALGIGGYFYYQQQTNKPQIHYITQAVTRAEI
                     NKNVLATGSVRANKRTEVGAQVSGKIQKLYVELGQSVKQGDLIADIDSSNQSNTLSTA
                     QARLSSYKAQLTSAQVALEVAQSNYNRLNKLYRANSASLNDLETAKNTLASAKATVDN
                     IKAQLKSAEISVNDAKTNLTYTQIISPMDGVIVSVPVSVGQTVNSNQTSPTIVQVADL
                     SKMLIKLEISEGDIAQVKVGQKVKFSTLAEPNREYSATIDSVDPALTTLTDNNYTEQS
                     GNTEAVYYYANVSVDNADNSLRIGMTTQAQITIDKREQVLVVPTSVIKKRGKENYVLV
                     LENEQSVERKIQIGISDTQNTEVLAGLNEGDQVIVTQRADDEKISTARGPRMF"
     gene            complement(17320..18135)
                     /locus_tag="PXB27_001041"
     CDS             complement(17320..18135)
                     /locus_tag="PXB27_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL5345844.1"
                     /translation="MTLPFRAIISDLDGTLLNANHKIGDFTIDTLEQLSKKGVDIFFA
                     TGRNHPDVSQIIGKVNVKNAMLVTSNGARANNLEGKKILNHYLPEDLAFELMNIEFDD
                     QNVCLNSYQGDEWFINKEIAELKKFHQDSGFSYQVVNFAHHHGRNTEKVFFIGKTAEA
                     LVPIEHYIRDTYGDRVQMTYSALLCLEVMAKGVCKANALAELVKLRGYTLADCIAFGD
                     GMNDVEMLSLAGKGCLMGNADPRLKAVLPNNEVIGFNKDEAVASYLRATFGIV"
BASE COUNT         4906 a         3800 c         3943 g         5498 t
ORIGIN      
        1 gaataggtat tcgagaaaac aagcggtcag agttgcaaaa ttttttactt atctgaccgc
       61 ttgttgtata gggaatatta ctttatttca ttgatcacat tcaaaatccg cttatccgaa
      121 atcgggtatt ttgtgccgag ctgttgagcg aacagactga cccgcagttc ttcgatcata
      181 tatcgaattt ccagtacctc gtccggcacc gctttggatt ttggcagttt ggcaaggagc
      241 tgtttatatg cctcttgcac tgcttccacc cgtagcattt tggctcggtc ggtgttgctg
      301 tccaccaaca gtttatccaa acggcgttcg atagcggtca aataacggta taaatctgca
      361 aggcgggcgt agccggtttt ggtgacaaag tcggggtaaa tcagcccttc gatttgggtt
      421 ttgatgtcgc ttaacgcaaa cgccatcgta aaatcgagct tgccttttaa ccgcttgttt
      481 agctcgtagg ccagtgtgag gcacttttca acctgtttgg caatttctac ggttttatcg
      541 tttaaatttg cccgcacaaa ttcgtgcagt tgggtgaatt tttcctccgt ccagacaaac
      601 ccgccgaact cttctaccag ctgatctacc gcacaggcaa tgcagtcgtc aatcagctcc
      661 aatactttgc cgaacggggc aaaatataag cccagcttgg ctttgttcgg cagtttttcg
      721 tgtagatatt taatcggcga tggcacattt agcaacagca gacggcgaag tcctgcctgc
      781 attgcacgag cctgctcaaa ttccgtttca aacagtttca cccccacagc cgtttgttca
      841 tcgacaatcg cagggtaggc tttcacgctg aaatgggctt ttttctgctc atagaactgt
      901 ggcaattcgg caaaattcca aatatgcaca ccactttgct caatgccgtc atcggcaatg
      961 ttggaaaggg tgttctgcac ctcatctttc aaggcaaatt tcagctcatc aaggttttcg
     1021 ctctcttgca gtttcttgcc tttatcatca atcacacgga aagtcatccg caaatgtggc
     1081 ggcagttggc ttaaatccca ctgttcaggc tcgaccaata cacccgtcat tcggcgtagt
     1141 tcgtggctta gattgtccag caacggtttt ttaaacggct cggctcgccc taaaaaggcg
     1201 tccgcataat tcggcgccgg cacaaaattt cgccgtagcg atttcggtaa agatttaatc
     1261 agcgagacga tcagctcgtg gcgtagcccc ggaatttgcc aatcgaaacc ttcgggttcc
     1321 acttggttaa gcagcggtag cggaatatgc accgtcacgc catcgtgatc ttttccgacc
     1381 tcaaactgat agctcagttt gagttttaaa tcgccttgat gccagaaatt cgggaaatcc
     1441 agctcgctgg caaggttggc gttgtcgttg atcaggaacg atttttcaaa gttcagcagt
     1501 tcaggatctt ttttgcttgc ctgtttccac caagtatcaa aatgcttgct cgacacaact
     1561 tcggtgccaa tccgctgatc gtaaaactca aacagcacct gctcgtccac caaaatatca
     1621 cggcggcggg atttgtgttc caatgcctca acttcacgga tcaagcggtg attttctttg
     1681 aaaaatttgt aattgttgtg acattcgcct tccaccaacg ccgagcggat aaaaatctca
     1741 cggctgacgg ttgggtctat cgagccataa ttcaccgagc gttgtgccac aatcggaatg
     1801 ccgtagagcg acactttttc cgatgccatt accgctcctt ttgacttcgc ccagtgtggt
     1861 tcgctgtaac tggatttagt gaggtgtact gccaacggtt ccacccattc cggctcaatg
     1921 cgagcgacca ttcgtcccca aagtttggta gtttccacca gctctgcggc cattacccat
     1981 ttcggctgtt ttttgaaaag tgccgagttc gggaaaatga aaaagtgggc atttcttgct
     2041 cctaaatagt gcattttttc attatctttc aagccgatat gcgacaacaa gccggcgagc
     2101 agggcggtgt gaatcggctg ataacttgcc ggctcactgt taatttttaa gcccatttct
     2161 ctaactgcaa ggcggagttg gtggtagata tcctgccact cccgcacacg gttgtagttc
     2221 aaataatcct tacggcagag attacggaac tggtttttcg acagttcttt ttgttgggct
     2281 tggataaagt gccacaaatt cacaaaggct aagaaatcgc tctctttatc ggcaaaacgg
     2341 cggtgtttat cgtctgccga ctgctgtttt tcctgcggac gctcacgagg gtcttggatt
     2401 gagagagctg acacaatcat catcacttcg tgcaacgaac cgtttttcgc cgcctcgatc
     2461 accattctgc ctaagcgagg atcgactggc agttgggcga gttggcgacc gattggggtt
     2521 aatctaaacc catccccccg tttacggggg gaggttggga gggggggagt gccatcatta
     2581 gaacggattt cccccctccc tagccctccc ccgcaagcgg tggaggggat tttttcattt
     2641 ggaataatcg cctgtaattc ttccaacaac cgaatcccgt cctgaatttg tcggctgtca
     2701 ggggcatcaa caaacgggaa attggcaata tcactcaatc caagcgaagt catttgtaaa
     2761 ataaccgctg caaggttagt tcgtagaatt tcaggatcgg taaattgtgg acggctgttg
     2821 aaatcctctt ctgaatagag gcggatacag atcccttccg aggttcgccc acaacgccct
     2881 ttccgctggt tggcggaggc ttgcgaaatc ggctcaatcg gcaagcgttg cactttggtg
     2941 cggtagctat aacgagaaat acgagccgtg ccggtgtcga tcacatattt aatattcgga
     3001 atagtgaggg aggtttctgc cacgttggtc gccagaataa ttcggtttag cccaccgctt
     3061 ggctggaaaa tccgttgttg ttcggcagcc gataagcggg cgtaaagggg taaaatttcg
     3121 ctatgtcgca gttcctgctt ttgcaaggct tcggcagtgt cacgaatttc ccgttcaccg
     3181 ttcataaaaa tcagaatatc gccacgccct tcggcttgca gttcgtccac cgcatttaga
     3241 ataccttgca gttgatcttg atcttccact atcgggcgat aacgcacttc caccgggaat
     3301 gtccgaccag agacttcaat aatcggggcg ttgttgaaat gtttggaaaa acgttctaca
     3361 tcaatagttg ccgaggtgat gatcactttc agatcggggc gtttgtgcag aatttgtttc
     3421 agataaccca aaataaaatc gttgttcagc gaacgctcgt gagcttcgtc aataatcagc
     3481 gtatcgtact gatttaaata gctgtcgtgc tggatttccg ccagcagaat accgtccgtc
     3541 attagcttga ttaagctgtt ttcactgact tgatcgttaa agcggacttt gtaaccgacc
     3601 gtctcgccca gttccgattt cagctcttcg gcaatgcgag tcgccaccga acgtgcggca
     3661 attcggcggg gctgggtatg cccaatcaag cccttcaccc cacgcccgag ttctaagcac
     3721 attttcggta actgggtggt tttgcccgaa ccggtttccc ctgcgatcac caccacctga
     3781 ttttcgctaa tcagttttaa aatttcctca cggcgagcgg aaacaggcag atcagggtag
     3841 tcaattttca ggttttgggc gtgctgagtt cgtgcgaaaa aacgtgcctg acaagcggtg
     3901 agatcttgcg aaatttctgc caaaactgac cgcttgtgat cgatgttttt aatattttcc
     3961 gcccctttta agcgaccgag caggcggcgg aaatcggcgg tggtgatatt ttccagcttc
     4021 acaagcggtg gtttcttatg atttttttgc aaaggtgttt ccatttaatt tctcttttgc
     4081 attaagacct tgtgatttat tgtagggtgg gcgtaagccc acgtaacaga atagggattt
     4141 tatgcgtgga cttacgtcca ccctacatta ggttttacca acaatcagtg acaaaatccc
     4201 tgccgcaatc aacggcccga ccggtacgcc ttttaagaac gccacgccga tcaccgtgcc
     4261 gattaaaagc ccagttacca acaccggctg gctgcccatt aaattcacgc ccctgccacc
     4321 tagccacgct accagcacgc cagcaattat tgagagtgcc attttccagt tcaatagttg
     4381 ggcaagttcg ggaagcgtaa ttcgccctga aaccaagggg ctaagtacgc cgatggttaa
     4441 aatgataatc ccgattttta agccgtattg atcgacaaag gggataaatt tagaaagtaa
     4501 ggtttgttgc ataatcagca atacggcggc tgaaatagta accgcactgt tttggctgat
     4561 cagccctaaa ataatcagca ccacaagtag aagtgccact gcgttaaatt gtaaagtcat
     4621 aggattaaaa cgtaaaaacc cttacttcat agaagtaagg gagtggttaa ttaaacgttg
     4681 agctagttaa taaatttcat tattgccatt aaaaagccaa gaattgtgat atttaaggta
     4741 attgctttaa atatcgtggt attgatttca ttttttagct tttcaagttc aagttgcaaa
     4801 tcagcttttg ttgctagtgg gctttgactt tgctctaagg cttcatcgag agcattagca
     4861 aatgcctcag ccatttcagg tgtttgattg gcttcttgca agcgttttac aaaacgtaat
     4921 ttgtcaaatc gtaggctcat agctgcatcc atttcaatta tcctcttgtg gttaagttat
     4981 aaactaattt agcataatca caagcaggaa ttttaggaga ttttgtgatc ttcgtcgaaa
     5041 atcgacaatt tagtttgcca ccatcaccat tgccgggcga atcactcgtc cgtgaagcgt
     5101 gtagcctttt tgtagcactg tgctgacatg gttggcttca attccttcgg caggttgcat
     5161 tgaaatagct tcgtgcaatt cagggttaaa tgcctcgcct acttcgccaa ttgccaccac
     5221 accaaattta gccagtgtat tgatgaattc tttatgagtc atttcaacgc cgtcaactaa
     5281 cgcttgagtg gtttcatcgg tcacggcttt atcaagggca tctaatccac gttcaaggtt
     5341 atcaaccact gttaacaatt ctttggagaa tttttctaaa gcgaatttgt gagctttttc
     5401 aacatcttgc tctgcacggc ggcggatatt ttgaatttct gcttgagccc taagctgaat
     5461 atcttgctca cgtttgtcgg cttctgcaat ataggcttct aactcggcaa ttcgagcttg
     5521 agcgagagct aacggttctt cttgaggtgt attttgttct tccgcttgag tttcttcagt
     5581 tacaacttca ggttgaattt cttcttgttt tacttcttct tgtaagtgtt cattttgagt
     5641 ggtcattttt tatcctttaa aagactaatc tggaaatgta aatcttcctt aatatagcga
     5701 taaattaagg gatttcaagc atatctcttt ctttttgcga aaataattca ctttttaatc
     5761 agaaaaggct tgcaaaatac gggggggggc ttaaaatgcc ctagtctttt ccgcccttag
     5821 gcaaggctat attttttaat cacttttctt taacggagaa aacttaaatg aaaaaattag
     5881 cgggtttatt tgcagcaggt ttagcaacag ttgcattaac agcgtgtaat gaagaaaaag
     5941 cggcagagcc aaaagcaact gcggctgaag caaaacctca agcaacaaat caaacagttc
     6001 acctttatac ttggactgaa tatgtgcctg aaggtttgtt agatgaattt acgaaagaaa
     6061 ccggcattaa agttgaagtt tcaagtcttg aatctaacga aacaatgtac gctaaattaa
     6121 aattacaagg caaagatggc ggttatgatg taattgctcc atcaaactac tttgtatcta
     6181 aaatggcgaa agaaggaatg ttagcggaat tagatcacgc acaacttccg gttattaaag
     6241 aattaaacca agattggtta aataaacctt acgatcaagg aaacaaatat tcattacctc
     6301 aattattagg tgcgccgggt attgcattta atacggcaga ttacaaaggt gaaacattca
     6361 cttcttgggg tgatttgtgg aaacctgaat ttgcaggtaa agtacaatta ttagatgatg
     6421 ctcgtgaagt attcaacatt gcgttattaa aattaggtaa aaacccaaat acaactaatc
     6481 cggatgaaat caaagaagcc tttgaagagt tgaaaaaatt acgtccaaat gtgctttcat
     6541 ttacctctga taacccggca aactcattta ttgctggtga ggtttctgta ggtcagttat
     6601 ggaacggttc tgtacgtatt gcgaagaaag aaaacgcacc ggttgatatg gtattcccga
     6661 aagaaggtcc tgtgctttgg gtcgatactt tagccattcc ggcaaatgcg aaaaacaaag
     6721 aaaatgcaca taaattaatc aactacttat taagtgcaaa agtagctgaa aaattaacgt
     6781 tagcgattgg ttacccaact tcaaacgtag aagcattaaa agtattaccg aaagaaatta
     6841 ctgaagatac agcaatttac ccatctgcag aggttttaca aaaatctcaa tggcaagatg
     6901 atgttggtga tgcaattgag ctttacgaaa aatactacca agagttaaaa gcagcaaaat
     6961 aatagctgat taattctgaa gcctctaggg agcaatcttt agaggctttc tcttttgagt
     7021 tgcaaaaaat ttggcaaaaa cgaccgcttt ctaaggtaca atcataaaaa catacttaga
     7081 ttttgagaga ttaaaccatg aacttaagat ggcaagaata tcctgaaaat gcccgcaaat
     7141 tatttaaact atccctaccc atttttattt ctcagctttc agcttccgga atggggctgg
     7201 cggatattgt aatggctggc ttggtgagtg atgatgatgt ctcggcgatt gcggtaagta
     7261 attcgattta ctttccgctc tttctgtttg tgtcagggtt gctgaatgca attaccccaa
     7321 ccgtttctta cttaaacggc tcgagcaatc gccatttaat tgctaaccaa gtaaggcagg
     7381 gctattggat tgtgtttgca atgtctattc cattaatggc aattttccta aacagccatc
     7441 tgattttaga cttaatgcaa acaccggctg agttttcaat caaatcgcaa caatatttag
     7501 cagtaatggc agttgggctt attcctgctt tgctggcggt gagtttacgt tgtatgaacg
     7561 atggattatc taaccctaaa cctgcgatgc ggattacctt tttcggttta ttgctgaaca
     7621 ttccgctaaa ctatattttc attttcggca aatttggctt gcctgaaatg ggagcggtag
     7681 gctgtggtgt agcaaccgca attgtgaact tgattatgtt ttttatgttg ttgcattatt
     7741 gctacaccaa ttctgcccaa aaagatattc gtttgtttga gaaatggatt gatgctccaa
     7801 gcggtcaaat tttgctaaaa ctttgcaaat tagggctgcc gattgccttt gcgattttta
     7861 ccgaagtgat gctgttttcg acctcatcgc tgtttctgtc accacttggt tcacaggtgg
     7921 tggctagcca ccaagctgca ttgcaaacca gctcgctatt ttttatgatt ccgatgtcgt
     7981 ttggcattgc aaccaccatt atgatcggac aaacacttgg gcagaaaaat gtggaggggg
     8041 cgaaaatctt aagttaccac gcactgatta ccgcaaccgc tttagctatt attgccgcca
     8101 tcattattat tttgttgaaa aattttatcc cttttgcgtt taccaacaac ccgatttcga
     8161 ttgcgattac cgctcattta ttgatttttg cggcggttta tcaaataccg gatgccattc
     8221 aagcggtagc aaacggcatt ctgcgtggtt ataaacatac ccaaccgatt ttatacgtta
     8281 ctctgttttg ttattggggg gtgagtatgc cgctgggcta cattttggca agaaccgatt
     8341 ggattgtaca accaatggct gctcaaggtt tttggttagt cttttgtatc ggcttaagtt
     8401 tggctggtgg cttgttatta tggcaaatgc gaaaaataca agcggtgcca aaagcacaat
     8461 taattgcaaa attagaacgg attaaataac agattctctg ctatggaaca tagcagtgct
     8521 ttttactttt agaagataac tttgctgatg aaaaacctaa atcctatcca actacctttg
     8581 aataaatccg ttttaatcga agcgtcggca ggtacaggga aaacctttac cattgccaac
     8641 ctgtatttgc gtttattgct cggcattggt tgcaacccgt taacggtgga acaaattttg
     8701 gtggtgacat ttaccaaagc agcaaccgaa gagttgaaaa accgtatccg taaaaatatt
     8761 cagcaatgtg ccgacttttt aaaagatcaa gctgatggtt tggaagtgga aagtacaaaa
     8821 tcctatcgaa acaaccttga ttttttggca cagatttatc cgctcattcc gaatattcac
     8881 gaagcattgt tacgtttaag tattgccgaa cgggaaattg ataccgccag cgtgtttacc
     8941 attcacggct tttgtcaaaa aatgctggtt cagtttgcgt ttgagtcggg cgtgcggttt
     9001 gacttggatt tacagccaaa ccaaagtgat ctacttaaaa aattaagtga agaagtctgg
     9061 cgagagcaat tctatccgca agatttagcc atcacttatg cggttgccga acaacttggt
     9121 acgccggaat atgccctcaa tgcggtaagg cgttatttat ctactgaatt acctgaacca
     9181 aatgcctctt taaatcaaga tattgccgag catttggctc aatatcagca gtttattaat
     9241 gaggtgaaat cctattggaa aacgcattca gatgaactgg ttgaaactat ccgccacgat
     9301 ttaaagcata aaattacgct gcacggaggt tggtatcgag cgaccacttt tgaagccggt
     9361 gtggaagtga tgaaccagtg ggctaattcg caacataacc atttcccggc agggctttat
     9421 ttattcacta cagagaaaat tgcggctggc acgaaaaaga gctgtgagcc gatagattcc
     9481 cctcatcttg caaaaaatca ggcttttttg accgcttaca aggaacgttt tgaaaacagc
     9541 ttgtcagcaa tgttgcagta tcaattttta attgctctca aagcaaaatt ggcagagtac
     9601 aaacaatcgc acccacaacg cagttttgat gatttactca caatgctaaa ccaagcgttg
     9661 aaaaatctac acacgggcga agccttgtct cagaaaattc gggcgatgta tccttttgcg
     9721 atgatcgatg agttccaaga taccgattta acccaatatg aaattttcag ccaaattttt
     9781 atggcagaga atgcagagaa cggctttatt atgattggcg acccgaaaca gtcgatctat
     9841 aaattccgtg gggcggatat tttcacgtat ttgcgagcag caaagcaggc tgatgaacaa
     9901 ttcacattgg gcaaaaactg gcgttcgttg cctgaagtgg tgcaagcggt caataatttg
     9961 ttttcttttg caaatgataa ctctccgttt ctttatgagg atattggctt tcacccagtg
    10021 gaggctgata gccaaaaagg tcagttagtt agtgagcaag ggcattttgt aggctacatc
    10081 acggaaaaca gtaaattaga tgatttggct gaaatgtgtg cttatcaaat tcatcaacag
    10141 cttgaggcga tggctcaagg caaattcgga gttgaaaaaa acggcgaatt tcaatccttt
    10201 gctgcaaaag atattgcgat tttagtccga aaaggttcac aagctaagcg aatcaaaaag
    10261 gcgttggcaa aacgtaatat caaatccgtc tatttagctg aatcagcttc tgtatttggt
    10321 tcggaaattg caacagaatt gtgttggtta ttgcaagccg cactgaatcc gtatcattac
    10381 aaggcattat tatcggcgtt aggctcaacc ttgtggggct taacggcggc agagatccac
    10441 cgctataagg aaaatgagca actttgggat ctgaaagtca atcagtttgt ggaatacagc
    10501 aacatttgga aagagcaggg cgttctgccg atgttgcata aaatctactt ggagcagggc
    10561 attatcgaac gtttgaaagg tttggaaaat gcggatcgct tgattacgga tttattgcat
    10621 ttaaccgaaa ttttgcagga aacctcgcaa gcgttagagt ctgaagctga actgttgcgg
    10681 tggtatgaaa agcaagttaa ttcgcccgat tttaatgacg agcaacaact tcggttggaa
    10741 agtgaagagg agctaatcaa aattgtgacg attcacggct cgaaaggctt gcaatatcca
    10801 attgtttggt taccttttgt ttcacaaaaa agtgagggag acacgcagac agcattaagt
    10861 ttatatcgca attctcaagg tgcgctggat tggtgtttag gctcagaaga ggcaagtttt
    10921 tcgctacagt atgaggctta tgcggaagat ttacgtttgc tgtatgtggc attaacccgc
    10981 gcggaatctc agcttaactt agtgttgccg aaagagattt ccggctggtc tccactccat
    11041 tacttgttag cagacgggga aatacctaca ggaaaagtgc ctgtttcaga ttattttagc
    11101 caaaaacgga ttgctgctaa tttggttgaa gtagccgggg aattaccttt ctcactgtgg
    11161 gaaggagaac aatcttcaga cgattaccaa gcaaaaacct ttagtggcaa tatccgtgtg
    11221 gttggtcaaa ttaccagctt tacttcactt tatgcccaac acgagcgttt tagtgaaggg
    11281 aaatcagaaa gggtaatgga tttagcccaa gatcgagatg tacatataga aaccattgag
    11341 gcaggttatt caagtgaaac tgccgctttc tcgccattcc aattcccaca cggtattaaa
    11401 gtgggcacgg tgttacatca cttttttgag cattgccaat ttaacgaaca aattgataga
    11461 gaggctgttg ctaaagtatg cgagcaatta gggctaagcg aggaatggat tgaaccaaca
    11521 gcattatggt ttgagcgtat tctaactacc cctttggcag aggcaaattt ctgcttaaaa
    11581 gcgattgatg aaaccaaacg attaaacgag tggcaattct atttacgctt aaaaaatgat
    11641 aaagccttac atcaattaaa tgcgttatta aagcaacata gccctttagc aaaaacttta
    11701 cctgaattgc aattaccgca attagaaggt tttgtgcgag gctttgtaga ttgtatcgtg
    11761 caagtggagg agaaattcta cttaattgat tacaaatcga atttcttagg ctatctgcca
    11821 caaaattatg cgaaagaaca cattcaacgg gaaatgggca gacaacgcta tgatttgcaa
    11881 tatctgcttt acactctcgc cttacaccgt tatctaactg cccgtttagg tgaaaaatac
    11941 gattatgaac gagacttcgg cggagtggct tacttgttct taagggcaat gaacggcgaa
    12001 aatgataacg gcgtttattt tgacaagccg actaagcagt tgattgaaga aatggatcgg
    12061 ttgtttgggt aaatatgtgt aggggcgaaa atttttcgcc cctttatggg taattaactc
    12121 gcttgctcat accaaatctt attgatataa agcaacactt taccaagcgg agtttctacc
    12181 ctaatttcat catcaacctg tttgccgatt aaggctcggg cgacagggga gtcaatcgaa
    12241 atccagtttt ttgctgggtc gaactcatcg caaccaacaa tacggtactg ttttgcctcg
    12301 ccctcttcat tttctaattc cacccaagcg ccgaaaaaga ctttgccttc ttgtttaggg
    12361 tgataatcaa caatttgtag cacctctagc cgcttagata aaaaacgcac acggcggtcg
    12421 atttctctca aacggcgttt gccgtaaata tattcggcat tttcgcttcg atcacccaat
    12481 gcggcagcat cagacaccgc ttgagtcact ttcgggcgtt cttcccgcca tagaaatttt
    12541 aattcctgat ctaaggcttg ccagcctgaa cgggtaatgt aatttgattt agccatagtt
    12601 atttaattgt ttatggagca aatagtgatt tgcccctacg aagtttctat tttgaaagca
    12661 ataaattcgc tactgttctt attcccagtc cggttgctcc tgccgcccat aaatcgctcg
    12721 ccgttttgcg gaatgtggca gaggcgtcaa tatgtagcca gttttgctgg tagttttcta
    12781 caaagtaaga taaaaatgcg gtggcggtac ttgccccagc ccccaccggc acagagccga
    12841 tattggcgat gtctgcaaag gaagaagcaa tttgcccacg gtgaaattct tcaaacggta
    12901 aacgccagaa cggttcattt tccgttttgg cagacttaaa tagtgcttcg gttaggttgt
    12961 cgtccattga aagcaccgag tggtagtcat tgccgactgc gacttttgct gctcctgtga
    13021 gggttgcagc atcaataata aattgtggat tttgcttgca cgcatcaatt aaaccatcgg
    13081 caagcactaa acgcccttca gcatcggtgt ttaaaacttc aacagttacg ccattgcggt
    13141 aagtgataat atcgcctaat ttgaacgcat tgtggctgac catattttcc gcacagcaga
    13201 ggtagagttt cactcgttta ttcaagcctc gagcaatggc aaaagcaagt gagccggtta
    13261 aaagtgccgc tccgcccata tcggttcgca ttgtgctcat actatcgctt ggttttagac
    13321 tataaccgcc ggtgtcaaag gtaatgcctt tgccgaccaa gcaagccagt acctgtgcgt
    13381 tttcatcgtt ggttgggttg aagtctaata ctaagaatgc aggcggattg gcagagcctt
    13441 ttccaactgt ccaaatgccg tgatagcctt gttcttgtaa tgcttcgcca cgaatgatct
    13501 ggtaagaaac cgtattcggt tttgccattt tttggataaa ctcgaccgct tgttcggcta
    13561 atttttcagg ggttaattta tctgatggct tattaattaa ttcacgcacg aaagtggcac
    13621 attttacacg gttatcaaat tcggcttgct gttcgtctaa gtctgggtat tgtaccgagc
    13681 cttgattttt taccgaatga aatccttgat gaaacgccca gcaggattct aaatcccaac
    13741 tatcgccggt cagctccacg gttgaaatac cttgattttt aattttgcgg gcggcttttt
    13801 gaatagtggc taaatcgttg ttcggattat gtacggtaaa ccccgcatca tttgcggaaa
    13861 gaatagcatt tttaccccaa atttcagggg cgttttgagt agaaagtgaa attttcattg
    13921 ctgtcattat cggtttcctt agaaaatgga ttttgcttta tcctaacaaa cttttgcaaa
    13981 gttttataca aaaatgaccg cttgcagagc atagccttga caagcggtca ttgtttcgtg
    14041 tttttacaac ctgattaagg acggattatt tgccgtccca agctttaatt gcattgtggc
    14101 ggattttaac acggctttca gcattgcctt tgtagtaatc tttatctaaa ccgccttccc
    14161 agtcgccata gttcgggttc ggtaacataa tgaatttttt accgaattgt tggctgtttt
    14221 cggctacgaa tgcacggcgt tccgcgtttg atttacggta agtcgcatcg ccgaaatcgt
    14281 ttaagttgtc gccaatatag agcacgattt catagccttg tttttcgatt tcttcaaagc
    14341 gaggggtttt gttcgatttg tcttttttca acaagagtgt ttgttcgctc acaccactaa
    14401 agcctaattt gttcatatca tcaatagtac cggctttttc actgctgtct ttacggtttg
    14461 aaacatagaa catagttcct ttgtggctat taacgtagtt gttaaattcc actgcaccgg
    14521 caatcgctcc ggtttggcgg gcatttaccc aacgagtcca gctttcacca tcaaacggct
    14581 tgtggttttt aacctgccag ccggcataaa ggctgttgtc caccatagtt tcatctaaat
    14641 ctactaccac ggcttttttc ttgccttttt tcactttggc ttggtcgaat gccactttcg
    14701 ctgcattgaa tgcttgatgg gcaagagctt ggtattcgcc ggattgttgc atccagttta
    14761 tccctaatgt agcttgctct tgcaacacca tttgatgatg ctcatcgctg tttttatgcg
    14821 tgtgcgaaca agcagttaat acaaataaag ctgtgaggct catagcagaa agtttgatgt
    14881 ttttcatacg tatatcctta gttttattgt tgataaacag agcgattata cctaaaaaga
    14941 gttgcttatg gctaatagtt tattgctcct attcactttt aaagcaaact aaggtagcct
    15001 agtaaccttt ttataacaat tacacagagg ctatatgcaa acattattaa aatttacaca
    15061 atttatcagc aaaaccttcg ccatttgggt ggtgtttttt gcttttatcg cagcacaatt
    15121 tccggaaacc tttaagcaat tcctgccttg gattccttat ctgttaggga ttgtgatgtt
    15181 gggaatgggg ttaaccttaa ctttcaaaga ttttgccgag gtaacgaaaa atccgaaatc
    15241 agtgattatc ggcgtgatcg ctcaattcgt ggtatgccga gtgtagctta tggattggca
    15301 aaagcattta acttacccgc agatttagcc attggtgtga ttttagttgg ttgttgcccg
    15361 ggcggtactt cttcaaacgt gatgacctat ttggctcgtg gcaacacagc gttatcggta
    15421 gcttgtacca ctatttcaac tttacttgcc ccagtgctca cgccggcaat tttctattta
    15481 tttgccagcc aatggttaga aattaatgca acggcaatgt ttatttcggt gttgcaaatg
    15541 gtgctattgc caatttttgt gggcgtggtg attcgtacta ttttcaaaca aaaagtagag
    15601 caatttagcc aaacaatgcc attaatttca gtgattgcaa tcgtattaat tgtcacggcg
    15661 gtggtgtcag ttagccgaga tcgtattatt gaatccggtt tattaatttt tgcggtagta
    15721 gcattacaca acggtttagg ttatttaatc ggttatttgg cgagccgttt attaaaatta
    15781 ccgattgcag acagcaaagc ggtggcgatt gaagtgggga tgcaaaactc aggattgggg
    15841 gcagcacttg ccgcattaca ctttaaagcc aaccctatca ttgcggtgcc aagtgcggta
    15901 tttagcttct ggcacaatat ttcaggcccg attttggcga tgattttcgc taaaatgaaa
    15961 aacgataacg aaaaataaaa ttcgttttaa tacagacgtt ttaatgtttt aataaagaat
    16021 agacaaacgg ttaaattttt ctaatttttt gcaaaatgtt cagaaaattt aaccgcttgt
    16081 tgtttagaat ttagaacatt cttggtccac gtgcagtgct gattttttca tcatccgctc
    16141 tttgggtaac aatcacttga tcaccctcat ttaagcctgc taatacttcg gtattttgcg
    16201 tatccgaaat gccaatttgg atttttcttt caaccgactg ctcattttcc agcaccaaca
    16261 cataattctc tttaccacgt tttttgatta cgctggtggg tacaactaaa acctgttccc
    16321 gtttatcaat agtaatttgg gcttgagtag tcattccgat gcgtaaactg ttatcggcat
    16381 tatcaacgga aacattagcg tagtaataaa ccgcttcggt attacccgat tgttcggtgt
    16441 agttattatc ggtcagcgtg gtgagtgccg gatcaactga atcaatggta gcactatatt
    16501 ctcgatttgg ctcagcaagg gtggaaaact taactttttg cccgacttta acctgtgcaa
    16561 tatcgccctc cgaaatttca agtttaatca gcatcttaga taaatctgct acctgtacaa
    16621 tagttggcga agtttgattg gaattgaccg tttgcccgac agaaaccggt acagacacaa
    16681 tcacgccgtc cattggcgag ataatttgcg tataagttaa gttggtttta gcatcgttta
    16741 ccgaaatttc agccgatttt aattgtgctt taatgttatc caccgtggct ttggctgaag
    16801 ctaaggtatt tttagccgtt tcaagatcgt ttaaagaggc actatttgcc cgatagagtt
    16861 tatttaagcg gttgtagttc gattgagcta cttcaagtgc aacctgtgca gaggtgagct
    16921 gtgctttata agaagaaagc cgggcttgag cggtgcttaa ggtgttgctc tggttgctgg
    16981 aatcaatatc tgcaattaaa tctccttgct tcaccgattg cccgagctcg acatagagtt
    17041 tttggatttt gcccgaaact tgagccccca cttcggtgcg tttattcgct cgaaccgaac
    17101 cggttgccag cacatttttg ttaatttctg cccgtgtgac agcttgggta atgtaatgga
    17161 tttgcggttt attggtttgt tgctgataat aaaaatagcc gcctattccg agagcggcaa
    17221 ttagcacaag agtggctaat gttttacgtt tcatactgtt ccttgatgat atacaagcgg
    17281 tcaaatttag acaaaaattt gcaaaaaagt tgctctcaac taaacaatgc caaatgtggc
    17341 tcgaagatag cttgccaccg cttcatcttt gttaaagcca atcacttcat tattcggtaa
    17401 tacggctttt aaacgtggat cggcattacc cattaaacaa cctttgccgg ctaaacttag
    17461 catttccaca tcattcatac cgtcaccaaa agcgatacaa tcggctaagg tgtagccccg
    17521 taatttgacc aattcggcga gagcattggc tttacacact ccttttgcca tcacttcaag
    17581 gcaaagtagg gcagagtagg tcatttgcac acggtcgcca taggtatccc gaatataatg
    17641 ttcaatcggt actaaagctt cagcagtttt gccgataaaa aacactttct cggtatttcg
    17701 accgtgatga tgagcaaaat ttactacttg atagctaaat cctgaatctt gatggaattt
    17761 ttttaactct gcaatctctt tattgataaa ccattcatcg ccttgatagc tgtttaagca
    17821 cacattttgg tcgtcaaatt caatgttcat taattcaaag gcaagatctt ccggtaaata
    17881 atggttgaga atttttttac cctctaaatt gttggctctt gccccgtttg aagtgactaa
    17941 cattgcattt ttaacgttta ctttcccaat aatctgagac acatctggat gattgcgacc
    18001 ggtggcgaaa aaaatatcaa cacctttttt tgatagttgt tctaatgtat caatagtgaa
    18061 atcaccaatt ttatgattgg catttaatag tgtgccatca agatcggaaa taattgcacg
    18121 aaatgggaga gtcataatct attccta
//
LOCUS       DANLUQ010000039        17988 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.039, whole genome shotgun sequence.
ACCESSION   DANLUQ010000039 DANLUQ010000000
VERSION     DANLUQ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 17988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17988
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.039"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            91..1053
                     /gene="ribF"
                     /locus_tag="PXB27_001042"
     CDS             91..1053
                     /gene="ribF"
                     /locus_tag="PXB27_001042"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HDL5345845.1"
                     /translation="MQLIRGFHNLDKHIALNQGCVLSIGNFDGVHLGHQNILARLCDR
                     ALDLGLPSVVMLFEPQPREFFAKKSGNSTACNPPARLMRLRDKLKYLEEAGVDFVLCV
                     RFSEKFAQISADDFISDLLAKRLKVRYLSVGDDFRFGAKRAGSFETLRNAGLKYRFAV
                     EESHTHSLNEERISSSLVREALQNNELDLAEKLLGKPYSILGRVAHGNKLGRTIGFPT
                     ANIMLNRLVTPIQGVFAVKVKTKQGSFNGIANVGNRPTINGTKPLLEVHIFDFNASIY
                     GEAIEVIFLKKLRNEIKFPDFEALKAQIEKDRLQAVEFFKEFAR"
     gene            1156..3972
                     /gene="ileS"
                     /locus_tag="PXB27_001043"
     CDS             1156..3972
                     /gene="ileS"
                     /locus_tag="PXB27_001043"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDL5345846.1"
                     /translation="MTIDYKNTLNLPETGFPMRGDLAKREPDMLKNWYDKNLYQKIRT
                     ASKGKKTFILHDGPPYANGNIHLGHAVNKILKDMILKSKTALGFDTPYVPGWDCHGLP
                     IELKVEGIVGKPNEKISAAEFRQACRDYAKEQVEGQKADFIRMGILGDWDNPYLTMNF
                     NTEANIIRTLGKVIANGHLYKGSKPVHWCLDCGSSLAEAEVEYEDKVSPSIYVRFNAV
                     DVNAVEEKFNAQGKGRGALSAVIWTTTPWTIPSNRAIAINPELDYALVQLGDERVLLA
                     VDLVEDVAKAAGVESAEILATTKGENLELLRFNHPFYDYSVPFIFGDHVTTDGGTGLV
                     HTAPDHGADDFVVARKYNIEMAGLISNDGKFKADTPFFAGLGVFESNDKVVEKLQEVG
                     ALLKLSRIKHSYPHCWRHKTPIIFRATPQWFIGMETQGLRQQALGEIKSVRWIPSWGE
                     ARIDTMVANRPDWCISRQRTWGVPMAMFVHNETEELHPRTLELIEEVAKRVEEKGIQA
                     WWDLDPAELLGEEAKDYRKVPDTLDVWFDSGSTYASVVEQRPEFNGNSADMYLEGSDQ
                     HRGWFMSSLMLSTATNGKAPYKQVLTHGFTVDEKGRKMSKSLGNVIVPSEVWNKNGAD
                     ILRLWVASTDYTGEITVSHNILNSAGESYRRIRNTARFLLANLNGFDPKSDLVQPQEM
                     IALDRWAVACALEAQNDIKEAYDNYQFHTVVQRLMRFCSIEMGSFYLDIIKDRQYTTK
                     ADSLARRSCQTALWHIAEALVRWIAPILSFTADEIWGYLPQVEGRSEFVFTEEFYTGL
                     FGLTEQDKLDDAYWQQILKVRAEVNRVLEQARKDKVIGAGLEAKVTVFANDEIRPLLE
                     QLGNELRFVLITSQAIVKPLAEADIAEGELAGLAVKVENADGEKCPRCWHYATDIGSH
                     SGHEEVCGRCVENVAGEGEKRLFA"
     gene            4097..5644
                     /locus_tag="PXB27_001044"
     CDS             4097..5644
                     /locus_tag="PXB27_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HDL5345847.1"
                     /translation="MMKIIAFLFIFLSVFSHTALANSMAEQCVALKSNSPEKTAIYKA
                     EWVEKSAELPAYCLVQGEIGKRVEVDNVPYGIQFELRLPEQWNEKFLFQGAGGVGGVI
                     FPAIGKLYPHGASGANGLNRGYAVVSNDSGHATRDLVFTADPEARSNYAYASIGKVTT
                     VAKQLIAEFYRKPSKHNYIMGCSNGGREAMMAAMRYPQEFDGVIAGSPGFRVSRSVLA
                     EVWDNRALLAVAPKNGDGDKILSQALTQQDLDVIANGVLTRCDKLDGLADGLINAWEQ
                     CDFQPEMVAKQLGQKKVALIKTIFEGAKNSRGELIYSPWAYDSGINAKGWRDWKLGDS
                     QTAQPNSISFKMGLKSLTHYYLAPRVPSRDPLSVDLDQASEQVKAVGSIHDTDQVDLS
                     AFKQNGGKMLIYQGVSDPIFSAVDLKNWYQSLQQAVENPQHFAKLFFVSAMNHCGRGA
                     TVNDFDMLTALENWVEKGLEPESIAAKAGELYPNKAKQIPLCAYPKVAIYQTGEDPNR
                     LTSYQCR"
     gene            5714..7081
                     /gene="pssA"
                     /locus_tag="PXB27_001045"
     CDS             5714..7081
                     /gene="pssA"
                     /locus_tag="PXB27_001045"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HDL5345848.1"
                     /translation="MLILNKQRKAEYLLKSLPYLPQVAEQVQFLASSKAFKQQILQLI
                     QTAKKRIYITALYFEKDEAGQEVLAALYQAKRNNPELEINILVDWHRAQRGRIGEAEN
                     LSNADWYAKVKYEQGLPAGQEIAFYGVSINGREVFGVLHLKGFVFDDTILYSGASINN
                     VYLHQFERYRYDRYHVIENKVLADSWVTFIQQHLLTNEAVKRLDTENRPKTAEIRAQI
                     KHFRKQLSREAYLFNGVAENGRLAVSPLVGLGRRKNQLNKVIEALFYQTEQKLTICTP
                     YFNFPHSLRTRIEWLLANGKSVEIIVGDKTANDFYTKPDEKFTMASALPYLYEKNLRA
                     FAKRLDGYVQNKQLTIRLWKDGENSYHLKGVWVDNRYILLTGNNLNPRAWRLDAENAI
                     LISDPQHQLSEKAETELNQICQHTQILTHYSDLEVLTDYPENVRKLLKKFGRVKLDKI
                     VKMLL"
     gene            7136..7879
                     /gene="rlmB"
                     /locus_tag="PXB27_001046"
     CDS             7136..7879
                     /gene="rlmB"
                     /locus_tag="PXB27_001046"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDL5345849.1"
                     /translation="MSEQIYGIHAVKAFLDNAPERLIEVLVLKGREDKRLMPLLNELQ
                     RLGVAIQQVNRQTLDNKSQGEVHQGIIARVVPQKELNEHDLEAILSQKKNPLLLILDG
                     VTDPHNLGACLRTADAAGVDAVIVPKDKSAQLTPTARKVACGAAEVMPLIRVTNLART
                     MRDLQERHNVWIVGTAGEAESGIYEAKLTGSIALVMGAEGDGMRRLTREHCDQLISIP
                     MAGSVSSLNVSVATGVCLFEIVRQKLAGQ"
     gene            complement(7966..9378)
                     /locus_tag="PXB27_001047"
     CDS             complement(7966..9378)
                     /locus_tag="PXB27_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5345850.1"
                     /translation="MKLLDIQNLSVETENRLQLVEPISLQLKAGKNITILGETGSGKS
                     LLIQAIMGALPDGLIASGEISVKNCKISTESDRLQTLWGKTLVMLPQEPRRSLNPIMA
                     IGKQLWESFHYVAGKPSSQAKTESKAYLAKLGLGESVENYPHQLSGGMAQRASFAIAT
                     AAGGKILLADEPTKGLDPISKGKVIELLKSAYQNGGGLLTITHDIEVAEQLGGTILVM
                     KKGELLEQGEAKALLNNPQHPYTKALISADPKHWQPLQDLQNLSQKQPLVSVKNLSIA
                     RGKKTLFSNLSFDLHKGEVLGIVGHSGIGKSSLADVLCGLLKPKSGEVVWHSHSHKKH
                     QVLKLYQDPPEAFAPNVSLKTLLDDVINKHQLDRLPIPSLLQQLSLNPEILQRNAENV
                     SGGELQRVAILRALLLEPVLLFADEVTSRLDPITQQETIELLINQCRARNCSLILVSH
                     DPYLIEKSCDKVINLEEYVG"
     gene            complement(9467..10249)
                     /locus_tag="PXB27_001048"
     CDS             complement(9467..10249)
                     /locus_tag="PXB27_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5345851.1"
                     /translation="MKISISQYLGGTILLAMLGFAFLQPYFYPMDIGFQDLTNILSNP
                     NEIAWFGTDHLGRDMLARLASAIRLSFGLSLFSVICALIAGLITGIMAGFFGGWIDRI
                     FSFICDLVMALPGLLLVLLFAALSPGSFWTLYLGISLVMWVEFFRVIRSISSTMANSS
                     EIQASRLMGMGWFYSFKRHLLPRLLPMIATLSAFSLGNAILALATLGFVSVGLRPPTA
                     ELGLMMTELFPYYYEAPLIFLQPVVAVFLMVLSLQLLSRKIK"
     gene            complement(10259..11191)
                     /locus_tag="PXB27_001049"
     CDS             complement(10259..11191)
                     /locus_tag="PXB27_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5345852.1"
                     /translation="MHKILFRRLIQILLVIWSVGTLTFILTRQLSGDMAYRIAASRYG
                     YDQADSAAAEAVRAELGLDQPWWQSYFSWLSDLAQFNLGKSLVTGDPVWSEIQHQFGH
                     TLSLAIVALILAILIGPPLGLLAARKPNGFFDRLTLVFSTLFRSVPAFIIAIGLITLF
                     SAQLKWLPAGGYGCWQHFILPALTLAIGLSTVSVRVTRSAMVQVKGSEFYQFSQLKGL
                     SRWHTFLRHGLPNIAVPVIAYHAVQLVYLIEGVVVVESLFAWPGSGHALVHAIIARDV
                     PMIQGTTLVMGGLFVVLNTIADLLSAWIDPRIKR"
     gene            complement(11411..12985)
                     /locus_tag="PXB27_001050"
     CDS             complement(11411..12985)
                     /locus_tag="PXB27_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5345853.1"
                     /translation="MKKWLIPLTVAAFCVSACDDKNSTQATAEKPQVENTQSQKNAEI
                     LPLATVVAPWEINSLDPNQSGVIFQRMNLAETLVEADLNGNLIPALATDWRSNEEGTI
                     WRFTLREGVKFHNDQVLTAQAVVNSLNIAKSKPGVLAKAFIKEIRAVDNKVEIELTKA
                     FVPFPPFLTHYTALILAPESYNEKGEVTQIIGTGAFKPTKIEAPQKLEAVQFEGYWGK
                     KPQVQQANYLASSRSETRMLMAQSEPSSLVFNLDTASVARLKADPNLQLVSGSIARTI
                     QLKMDVAKPFFNDLAIRQALSQAINRKAIAEQVLKIENGAADQILPKAFADWRVDTAE
                     QVVDIAKIKQNLTASGYTFDEQGRLTKDGKPFSFTMRTFSDRPELPVIATILQAQWKQ
                     IGVEVNVSVGNFSEIPAGHQDGSLEMALYALNYGKTLDPYGVIASDFANGGSDWGVMN
                     WQNTEVDNAVKSLETERDPQKAKALKQTVSKIIYNDLPIIPVVYYQQNVVAHKEVKNV
                     TLDPFERRFFLELLSK"
     gene            complement(13244..14824)
                     /gene="prfC"
                     /locus_tag="PXB27_001051"
     CDS             complement(13244..14824)
                     /gene="prfC"
                     /locus_tag="PXB27_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDL5345854.1"
                     /translation="MSYPQEVNKRRTFAIISHPDAGKTTITEKVLLYGNAIQTAGSVK
                     GKGSAQHAKSDWMEMEKQRGISITTSVMQFPYNDCLVNLLDTPGHEDFSEDTYRTLTA
                     VDSCLMVIDSAKGVEERTIKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVESVLK
                     IKCAPITWPIGCGKLFKGVYHLYKDETYLYQTGQGHTIQEVRVVKGLNNPELDQAVGD
                     DLAQQLRDELELVQGASHEFDHTEFIKGELTPVFFGTALGNFGVNHFLDGLTEWAPAP
                     QARQSDEREVSPSEEKFSGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLKHVRIG
                     KDVVISDALTFMAGDRSQAEEAYAGDIIGLHNHGTIQIGDTFTQGENFKFTGIPNFAP
                     ELFRRIRLRDPLKQKQLLKGLVQLSEEGAVQVFRPLINNDLIVGAVGVLQFDVVVSRL
                     KTEYGVEAIYETVNVATARWVEGKDAKKFEEFKRKNEANLALDGGDNLTYIAPTMVNL
                     NLAQERYPDVQFFKTREH"
     gene            15046..15921
                     /locus_tag="PXB27_001052"
     CDS             15046..15921
                     /locus_tag="PXB27_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDL5345855.1"
                     /translation="MMKKFIPVMIAALVLSACSSQPAKRKSTPRIKATAAYIPTENSA
                     YVVFDLNQKKFLEGKNFNLVRPIASVTKLMTAVVFLEENRYGASCQTQILPSDADFLK
                     GTTSPLPKNLNIACSELLKAMLVRSDNYAAHALAHATHLNKAQFLARMNSKAREIGMT
                     QTYFGDSSGLSSDNVSTVVDLAKLAGYAASKPEIQQLSNSPLVSVYAGGRYFSMKNTN
                     SMVRSQSYQALVSKTGYTREAGYNLAFIAKGDCRGKRIGVVSLNNDNAASRTDFTESM
                     LAKYQCVPNPYRQYD"
     gene            15994..16581
                     /gene="pth"
                     /locus_tag="PXB27_001053"
     CDS             15994..16581
                     /gene="pth"
                     /locus_tag="PXB27_001053"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDL5345856.1"
                     /translation="MSEIKLIVGLANPGVKYEETRHNAGEWLINELARIYNVSLKEEP
                     KYFGKVAKINTAQGEVRLLVPTTFMNLSGKAVGALANFYRIKPEEILVAHDELDLPPG
                     SVKLKKGGGHGGHNGLKDIIAALGNNKEFYRVRIGIGHPGHKDLVANYVLGKPSPSDQ
                     TLLNSAVDEAARCVEVLFKDGMVKATNRLNSFKAN"
     gene            16767..17858
                     /gene="ychF"
                     /locus_tag="PXB27_001054"
     CDS             16767..17858
                     /gene="ychF"
                     /locus_tag="PXB27_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDL5345857.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANYPFCTIEPNTGVV
                     PMPDPRLDALAEIVKPERVLPTTMEFVDIAGLVAGASKGEGLGNKFLANIRETDAIGH
                     VVRCFENDDIVHVAGKIDPADDIEIINTELALADLDSCERAIQRLQKRAKGGDKDAKF
                     ELSIMEKILPVLENAGMIRSVDLDKDELHAIKGYNFLTLKPTMYIANVNEDGFENNPY
                     LDRVREIAEKEGAVVVPVCAAIESEIAELDDEEKVEFLQDLGIEEPGLNRVIRAGYRL
                     LNLQTYFTAGVKEVRAWTVSVGATAPKAAAVIHTDFEKGFIRAEVIAYQDFIDNKGEA
                     GAKEAGKWRLEGKDYIVQDGDVMHFRFNV"
BASE COUNT         5150 a         3671 c         4197 g         4970 t
ORIGIN      
        1 aaaaaatata acagattgaa tttttttcct tttttgtaga tttctcgtta taatcacagc
       61 aattttcact tataaaatgg tttatctttt atgcaactta ttcgaggttt ccacaactta
      121 gacaaacaca tcgctttaaa tcaaggctgt gttctctcta tcgggaattt cgatggtgtg
      181 catcttgggc atcaaaatat tttggctcgt ctttgtgata gagctttaga tttaggattg
      241 ccctcagttg tgatgttatt tgagccacaa cctcgagagt tttttgcaaa aaaaagtgga
      301 aattcaaccg cttgtaaccc gcccgcccgc ttaatgcgcc tgcgagataa gctaaaatat
      361 ttggaagaag ccggtgtgga ttttgtgcta tgcgtgcgtt ttagtgaaaa atttgctcaa
      421 atttcggcag acgatttcat cagcgatttg ctcgctaaaa gactgaaagt acgctattta
      481 agtgtaggcg atgattttcg ctttggggca aaacgggcgg gcagttttga aactttacgc
      541 aatgccggct taaaatatcg ctttgcggtg gaagaaagcc atacccatag cttaaacgaa
      601 gaaagaatta gcagctcatt agtgcgagaa gccttacaaa acaatgagtt agatttggca
      661 gagaaattac tcggcaagcc ttattcgatt ctgggacgcg ttgcacacgg taataaactc
      721 ggcagaacca ttggttttcc taccgcaaat attatgctca atcgtttggt tacgccgatt
      781 caaggggtat ttgcggtaaa agtgaagacc aaacagggca gttttaacgg aatcgccaat
      841 gtcggcaacc gcccgaccat taacggtact aagccgttat tagaagtgca tatttttgat
      901 ttcaatgcct ctatttacgg tgaggcgatt gaagtgatct ttttaaaaaa attacgcaat
      961 gaaataaaat ttcccgactt tgaagcacta aaagcccaga ttgagaaaga tcgcttgcaa
     1021 gcggttgaat tttttaagga atttgcaagg tgagcctttc tagtttccag ccccagaggg
     1081 gctgagctat gcttaaccta gtacggctat gccgtgctag gcataaaaca gataatttta
     1141 acgagaaata aaaaaatgac tattgactac aaaaacacct taaacttgcc tgaaacaggc
     1201 tttcctatgc gtggggatct cgcgaaacgt gaacccgata tgttaaaaaa ttggtatgac
     1261 aaaaatttat accaaaaaat tcgtactgcc tcgaaaggca agaaaacctt tattttgcat
     1321 gatggtcctc cgtatgctaa cggtaatatt cacttaggtc acgcagtgaa taaaatttta
     1381 aaagatatga ttttgaaatc gaaaaccgca ttaggtttcg atacccctta tgtgccgggc
     1441 tgggactgtc acggtttgcc gattgagctg aaagtagaag ggattgttgg taaaccaaat
     1501 gagaaaattt cggctgccga gtttcgccaa gcgtgccgtg actatgcgaa agaacaagta
     1561 gaaggtcaga aagccgattt tatccgtatg ggtattttag gtgattggga taacccatac
     1621 ctaacaatga attttaacac tgaggcgaat attattcgca cgctcggcaa agtgattgcg
     1681 aacgggcatt tatacaaagg ttcaaaaccg gtacattggt gcttagattg tggatcttcg
     1741 ttagcggaag ctgaagtgga atatgaagat aaagtttctc cctctattta tgtgcgtttt
     1801 aatgctgtgg acgtaaatgc ggtagaagaa aaattcaacg cacagggtaa aggtagaggg
     1861 gcattatctg cggttatttg gacaactacc ccttggacta ttccgtctaa ccgtgcgatt
     1921 gcgatcaacc ctgaattaga ctatgcctta gtccaattag gtgatgagcg tgtgttacta
     1981 gcggtcgatt tagtcgaaga tgttgcaaaa gcagcaggcg ttgaatctgc tgagatttta
     2041 gcaaccacca aaggcgaaaa cttagaatta ttacgcttta accacccatt ctatgattac
     2101 agcgtgccat ttatttttgg cgaccacgtt accacagacg gtggtacagg gttagtgcat
     2161 actgcaccgg atcacggtgc ggacgacttc gtggtcgctc gcaaatataa cattgaaatg
     2221 gcagggctaa tttccaatga cggtaaattc aaagcagaca ccccgttctt tgccggctta
     2281 ggtgtgtttg aatccaacga taaagtagtc gaaaaattac aagaagtggg agcattatta
     2341 aaattatccc gcatcaagca tagttatccg cactgttgga gacacaaaac cccgattatc
     2401 ttccgtgcta ccccacaatg gtttatcgga atggaaaccc aaggcttacg ccagcaagca
     2461 ctaggagaaa tcaaatcggt gcgttggatc ccaagctggg gtgaagctcg tattgatacg
     2521 atggtggcaa accgcccaga ctggtgtatt tcacgccaac gtacttgggg cgtgccaatg
     2581 gcgatgtttg tgcataatga aaccgaagaa ttacatcctc gcactttaga attaattgaa
     2641 gaagtggcga aacgtgtaga agaaaaaggc attcaagcgt ggtgggattt agatcctgcg
     2701 gaattgctcg gtgaagaagc caaagattac cgcaaagtgc cggatacctt agacgtgtgg
     2761 tttgattcag gatcgaccta tgcctctgtt gtagaacaac gccctgaatt taacggcaat
     2821 tcagcggata tgtatttaga aggttcagac caacatcgtg gttggtttat gtcatcttta
     2881 atgctttcca ctgctactaa cggcaaagca ccatacaaac aagtgctaac tcacggtttt
     2941 accgtagatg agaaaggcag aaaaatgtcc aaatcgcttg gcaacgtaat tgtgccaagt
     3001 gaagtgtgga acaaaaacgg tgcagatatt ttacgcttat gggttgcctc caccgactat
     3061 accggtgaga ttaccgtttc gcacaatatt ttaaacagtg ccggggaatc ataccgccgt
     3121 attcgtaaca cagcccgttt cttattggcg aacttgaacg gtttcgatcc aaaaagtgat
     3181 ttagttcaac cgcaagagat gatcgcctta gaccgctggg cggtggcttg tgctttagag
     3241 gctcaaaacg acatcaaaga agcctatgat aactaccaat tccacacggt agtacagcgt
     3301 ttaatgcgtt tctgctcgat tgaaatgggg tctttctact tagatattat caaagaccgc
     3361 caatacacca ccaaagccga cagccttgcc cgccgtagct gccaaacagc gttatggcat
     3421 attgcagaag cgttagtacg ttggattgca ccgattttat ccttcacagc cgatgaaatt
     3481 tggggctact taccgcaggt ggaaggcaga agtgaatttg tctttaccga agaattctac
     3541 accggattat tcggtttaac cgagcaagat aaattagatg atgcttactg gcaacaaatc
     3601 ttaaaagtgc gtgcggaagt caaccgtgtg ctggaacaag cccgtaaaga caaagtaatt
     3661 ggtgctggct tagaagccaa ggtgacggta ttcgccaatg atgaaattcg cccgttatta
     3721 gaacaacttg gcaatgaatt acgctttgtg ctgattacct cacaagcgat tgttaaacca
     3781 ctggcggaag cagatattgc ggaaggcgaa ttagccggac tcgcagtaaa agtggaaaat
     3841 gcagatggtg agaaatgccc tcgttgctgg cattatgcta ccgacatcgg ctcgcactct
     3901 ggccacgaag aagtttgcgg acgttgtgtg gaaaacgtag ccggcgaagg cgaaaaacgc
     3961 ttgtttgctt aaaatgtaat tcaattcatc tttctaaggc agaggaaact ctgccttagc
     4021 ttttccagcg atagatagac actcagttta aaatttgcaa aaattttcgg aaaaataacc
     4081 gcttgtattg atgtaaatga tgaaaataat cgcttttctt tttatttttc tctccgtttt
     4141 ttctcacact gctttggcta attctatggc tgaacaatgt gtcgcgttaa aatctaactc
     4201 gccggaaaaa acggctattt ataaagctga atgggttgag aaatctgctg agttgccggc
     4261 ttactgttta gtacaaggtg aaattggaaa acgggtggag gtggataatg ttccttacgg
     4321 tattcaattt gagctacgtt tacccgaaca atggaatgaa aaattcttat ttcagggggc
     4381 gggcggtgta ggtggggtga ttttccctgc cattggtaag ctctatccgc acggggcgag
     4441 tggtgcaaat ggcttaaaca gaggctatgc agtagtgagt aacgacagtg gtcacgccac
     4501 tcgagattta gtgtttaccg cagatcccga agctcgctca aattatgcct atgcctctat
     4561 cggcaaagtg accacggttg ccaagcagtt gattgccgaa ttttaccgaa aaccgagcaa
     4621 gcacaattac attatgggct gctcgaatgg tggtcgtgag gcgatgatgg cggcaatgcg
     4681 ttatccacag gaatttgacg gtgtgattgc aggcagcccc ggctttcggg tttcccgttc
     4741 agtcttggcg gaagtatggg ataatcgagc cttgttggca gttgctccga agaatggtga
     4801 tggcgacaaa attttatccc aagccctcac tcaacaagat ttagatgtga ttgccaatgg
     4861 cgtattaaca cgttgcgata agttagacgg cttagcagac ggtttaatta atgcgtggga
     4921 acagtgtgat ttccagccgg aaatggtggc aaagcaactg gggcagaaaa aagtcgcttt
     4981 aatcaaaacg attttcgagg gggcgaaaaa cagtcgaggc gagctgattt atagcccttg
     5041 ggcgtatgat tccggcatta acgctaaagg ctggcgggat tggaaattag gtgatagcca
     5101 aaccgctcag cccaacagca ttagttttaa aatgggattg aaaagtttga cccattacta
     5161 tttagccccc agagtgccga gccgtgatcc actcagcgta gatttggatc aagccagcga
     5221 acaagtgaaa gcggtaggga gcattcacga tacagatcaa gttgatttat ccgcttttaa
     5281 gcagaacggt ggcaaaatgc tgatttatca aggcgtttca gacccgattt tttcggcagt
     5341 tgatctcaaa aattggtatc aaagcctaca acaagcggtc gaaaatccgc aacattttgc
     5401 aaaattgttt ttcgtgtcgg caatgaacca ttgtgggcga ggggcaacgg tgaatgattt
     5461 tgatatgctc actgcccttg aaaattgggt tgagaaagga ctggagccgg aaagtattgc
     5521 cgccaaagcc ggtgagcttt accctaataa agccaagcag atcccgcttt gtgcttatcc
     5581 gaaagtcgca atttatcaaa ccggagaaga tcctaatcgt ttgaccagct atcaatgccg
     5641 ttaagcgaaa atcgctttat tggcgatttc agtgtaaaat agcaggcaac gattgaaaat
     5701 tttggaatca atgatgttaa ttttgaacaa acaacgtaaa gctgaatacc tattgaaaag
     5761 cctgccatac ttaccacaag tggcagagca ggttcagttt ctcgcctcga gcaaagcgtt
     5821 taaacaacaa attttacagc tgattcaaac ggcaaaaaag cgtatttata taaccgctct
     5881 ttactttgaa aaagatgaag ccgggcagga agtattagct gctttatatc aagcgaagcg
     5941 aaataatcct gaactggaaa ttaacatttt ggttgattgg caccgagccc aacgtgggcg
     6001 tattggtgag gcagagaatt tatctaacgc cgactggtat gcaaaagtta aatacgaaca
     6061 aggtttaccg gcagggcaag agattgcctt ttacggtgtg tcgattaacg gacgagaagt
     6121 gtttggtgta ttgcatttga aaggcttcgt atttgacgat acgattttgt atagcggagc
     6181 aagcattaat aatgtgtatt tgcatcaatt tgaacgttac cgttatgacc gttaccacgt
     6241 cattgaaaat aaagttcttg ccgatagctg ggtgacgttt attcaacagc atcttttaac
     6301 caatgaagcg gtaaaacgtt tagatacgga aaatcgcccg aaaacggctg aaattcgtgc
     6361 gcaaattaag catttccgca aacaattaag tcgggaagcg tatttattta acggtgttgc
     6421 tgaaaatggt cgcttagcgg tttctccttt agtaggctta gggcgacgta agaatcagtt
     6481 aaataaagtg attgaagcgt tgttttatca aactgagcag aaattaacga tttgcactcc
     6541 gtattttaat tttccgcatt ctctgagaac ccgtattgaa tggctattgg caaacggcaa
     6601 atcggttgaa attattgtgg gggataaaac cgctaatgat ttctacacca agccggacga
     6661 aaaattcacg atggcatcgg ctctgcctta tttgtatgaa aagaacttac gagcctttgc
     6721 aaaacggttg gacggttatg tacaaaataa acagttaacc attcgcttat ggaaagatgg
     6781 cgaaaacagc taccacttaa aaggggtttg ggtggataac cgctatattt tgctgaccgg
     6841 caataacctt aacccaagag cgtggcggtt agatgccgaa aatgcaattt taatttccga
     6901 tcctcaacat caattaagtg aaaaagcaga aacggagtta aaccaaattt gccaacatac
     6961 gcagatttta actcactaca gtgatttgga agttttaacc gattaccctg aaaatgtgcg
     7021 taaattgctg aaaaaattcg gcagggttaa gttggataaa attgtgaaga tgttgcttta
     7081 gttaggtatt tatatgggcg agctgatggg ttcgcccaat tattaaggat aaaatatgag
     7141 cgaacaaatt tatggcattc acgcagtgaa agcctttttg gataatgcac ctgagcgttt
     7201 aattgaagtg ctggtgctaa aagggcgtga agataaacgc ttaatgccac tattaaatga
     7261 attacaacgt ttaggggtgg caattcaaca agtgaaccgc caaaccttag ataacaaatc
     7321 gcaaggtgaa gtgcatcaag ggattattgc cagagtcgtg ccacaaaaag agctgaatga
     7381 acacgattta gaggcgatct taagccaaaa gaaaaatcca ctcttgttga ttttagatgg
     7441 cgtgaccgac cctcacaacc taggggcttg tttgcgtacc gctgatgcgg caggggttga
     7501 tgcggttatt gtgccgaaag ataaatctgc tcagcttaca ccaaccgctc gcaaagtcgc
     7561 ctgtggggca gcggaagtga tgccactgat tcgagttacc aatctggctc gcacaatgcg
     7621 agatttacaa gaacggcata atgtctggat tgtcggcacg gcaggtgagg cggaatcggg
     7681 catttatgaa gcaaaattaa ccggctcgat tgcgttagtt atgggggcag aaggcgacgg
     7741 aatgcgtcgc cttacccgtg agcattgcga ccagcttatc agcattccaa tggcgggctc
     7801 agtatcttct ttgaatgtct cagttgcaac cggggtctgt ctgtttgaaa ttgtacggca
     7861 gaagctggct gggcaataag gaataagtaa gaaaaatgcg aagaactgat gtcttcgcat
     7921 ttttgttttt agagccgcaa aaaatctcag aaattgaacc gcttgttagc ccacatactc
     7981 ttccaaatta atcactttat cgcacgattt ctcaattaga taaggatcgt gactgaccaa
     8041 aatcagactg caatttctcg ctcggcactg gttgatcagc aattctatcg tttcttgttg
     8101 tgtgattggg tcaagccgag aagtgacttc atcggcaaac agtagtaccg gttctaagag
     8161 caaggctcgc aaaattgcta ctcgttgtag ctcgccgccg gagacatttt ccgcattgcg
     8221 ttgcaaaatt tctgggttga gcgaaagttg ttgcagtaaa cttggaatcg gcagtcgatc
     8281 taattggtgc ttgttaatca catcatcaag caaggttttt agactgacat tcggagcaaa
     8341 ggcttccggt gggtcttggt aaagcttcaa cacttggtgt tttttatggc tgtggctgtg
     8401 ccacacaacc tcgcccgatt tcggcttaag caaaccacaa agtacatcgg caagcgagct
     8461 tttgccaatc ccactatgcc cgacaatccc aagcacttcg cctttgtgta agtcaaagga
     8521 aaggttgcta aacagcgttt tcttaccacg ggcaatactc aaatttttaa ccgaaacaag
     8581 cggttgtttt tggcttaaat tttgcagatc ttgcaatggt tgccaatgtt ttggatcggc
     8641 actgataagt gctttggtgt aagggtgttg tgggttattt aacaacgctt ttgcctcacc
     8701 ttgctccaac agttcgcctt ttttcatcac taaaatagtg ccaccgagtt gctcggcaac
     8761 ttcaatatcg tgagtaatgg tgagcaagcc tccaccgttt tggtaggcgg attttaacag
     8821 ttcaatcact ttgcctttac tgattgggtc aagccctttg gtcggctcat cggcaagtaa
     8881 aattttgcca ccggcggcag ttgcaatcgc aaaagaggca cgttgagcca ttccaccgga
     8941 aagctggtgt gggtagtttt caaccgattc gcccagccca agtttggcta aataggcttt
     9001 gctttccgtt ttggcttgag atgatggttt gcctgccaca tagtgaaaac tttcccacaa
     9061 ctgcttgccg attgccataa tcggattaag cgagcggcga ggctcttgtg gcagcattac
     9121 caaggtttta ccccacaatg tttgcaagcg gtcggattcg gtggaaattt tgcaattttt
     9181 taccgaaatt tcaccgcttg cgattaagcc gtcgggtaac gcccccataa tcgcttgaat
     9241 cagcaggctt ttgcctgagc cggtttcgcc taagatagta atattttttc ctgcctttaa
     9301 ttgtaggcta atcggttcga ccagctgtaa tctgttttcg gtttcaacag ataggttttg
     9361 gatatcgagt aatttcatct tcattttatt actgtagggg cgggccctgt gcccgcccat
     9421 aaatataata agaattttgg gcaggcacag gacctgcccc tacgcattat ttaatttttc
     9481 tagacaataa ttgtaagctg agtaccatta agaaaacggc aaccactggt tgcaaaaata
     9541 tcagcggtgc ttcgtagtag taagggaata gttcggtcat catcaagcct aattctgctg
     9601 taggcgggcg aagccctacg ctgacaaagc caagtgtggc gagagctaaa atcgcattgc
     9661 cgagcgagaa tgccgataaa gtggcaatca tcggtaataa acggggcaat aagtggcgtt
     9721 tgaagctata aaaccagccc attcccatta ggcgtgaggc ttgaatttcc gagctgtttg
     9781 ccattgttga actgatagag cggatcacac ggaaaaattc cacccacatt accaatgaaa
     9841 tccctaaata gagcgtccaa aatgagccgg gagaaagggc ggcaaagagc aatactaaaa
     9901 gtaagccggg gagtgccatc actagatcac aaataaagct gaaaatgcgg tcaatccagc
     9961 caccgaaaaa gcctgccata atgccggtaa ttaagcctgc aattaaggca caaatcacgc
    10021 taaacagtga taagccaaac gaaagacgaa tagcagaggc aagacgggca agcatatccc
    10081 gcccgagatg atcggtgccg aaccaagcga tttcatttgg attggaaagg atattggtta
    10141 aatcttgaaa gccaatatcc atcgggtaaa aataaggctg taaaaaggca aatcccaaca
    10201 ttgcaaggag aatagtgccg cctaaatatt gtgaaattga aattttcatc ttgatgtcct
    10261 atcgcttaat tcgtgggtca atccaagcac ttaatagatc ggcaatggtg tttaatacaa
    10321 caaacaatcc gcccatcacg agcgttgtac cttgaatcat cggcacatca cgggcaatga
    10381 tggcgtgaac cagtgcgtgt ccgctgccgg gccaagcaaa taggctttcg accaccacaa
    10441 cgccttcaat taaataaact aattgtactg cgtggtaggc aatgaccggc acggcaatat
    10501 tcggcaaacc gtgacgtaaa aaggtatgcc aacggcttaa gccttttaat tgtgaaaatt
    10561 ggtaaaattc tgagcctttt acctgaacca ttgcagaacg ggtgactcgt accgaaaccg
    10621 tactcaagcc aatcgcaagc gttaaggcag gcaagataaa atgttgccaa cagccgtatc
    10681 cgccggcagg tagccatttt agttgagcgg aaaacagcgt aatcaagcca atcgcaataa
    10741 taaatgccgg cacggagcga aacagggtac tgaataccag tgtcaagcgg tcaaaaaagc
    10801 cgtttggctt acgggcggct aacaaaccga gcggaggccc gataaggatt gctaagataa
    10861 gtgccacaat cgcaaggctt aaggtatgcc cgaattggtg ctgaatttca gaccagaccg
    10921 gatcaccggt caccaatgat ttgcctaaat taaattgagc caagtcactt agccagctga
    10981 agtagctttg ccaccaaggt tggtctaatc ccagctcggc acgcacagcc tcagcagcag
    11041 cactatctgc ttgatcataa ccatagcggc tggcagcaat gcggtatgcc atatcacccg
    11101 aaagttggcg ggtgaggata aatgtgagtg tgccaaccga ccaaatcacc agtagaattt
    11161 ggataagacg gcggaagaga attttgtgca ttgtattatt tcatttatta aaaagatgat
    11221 tttatgcgtt ctgatccccc tctttagcaa agaggggaag ggaagatttg gcagaagaaa
    11281 taagcggaga aaataggtga aaaatgagaa aaaacgataa ttctgtcact atttattcac
    11341 ctaagagcgc aaattactac tgaaaaatcc taccttccct tttctaaagc gaggagtatt
    11401 ttgaattaaa ctacttactt aataactcca aaaagaagcg tctttcaaat ggatctagcg
    11461 tcacattttt tacttcttta tgtgccacca cattttgttg gtaataaacc accggaatga
    11521 ttggcaaatc attgtaaata attttgctaa ccgtttgctt taatgctttc gctttttgcg
    11581 ggtcacgctc ggtttcgagc gatttgactg cattgtctac ctcagtattt tgccaattca
    11641 ttacccccca atcactgcca ccattggcga agtcggaggc aattacgcca tatggatcta
    11701 aggttttgcc ataatttaag gcataaagag ccatttctaa actgccgtct tgatgccccg
    11761 ccggaatttc gctgaaatta cccaccgaaa cattcacttc cacgccaatt tgtttccatt
    11821 gagcttgtaa gatagtagca atcaccggta attcaggacg atctgagaag gtgcgcatgg
    11881 taaagctaaa cggtttgccg tctttggtta agcgaccttg ttcatcaaag gtatagccgg
    11941 aagcggtcag attttgttta atttttgcaa tatcgaccac ttgttctgcg gtatcaacac
    12001 gccagtcggc aaaggctttc ggtaaaattt gatcggcagc tccgttctca atttttaaga
    12061 cctgctcagc aatcgccttg cgattaatcg cttgcgataa ggcttggcga atcgctaagt
    12121 cgttaaaaaa cggctttgcc acgtccattt tcaactgaat agtacgggca atagatccgc
    12181 tgactaattg caggttagga tcggctttca gccttgctac actggcggta tctaaattaa
    12241 ataccagtga tgacggctcg ctttgtgcca ttaacatacg ggtttcgcta cggctgctgg
    12301 ctaaataatt ggcttgctga acttgcggtt ttttgcccca atagccctca aattgcaccg
    12361 cttccaattt ctgcggagct tcaatttttg tgggtttaaa cgcacctgta ccgataattt
    12421 gggtcacttc gcctttttca ttatagcttt caggtgccaa aattaaggcg gtgtagtgtg
    12481 ttaaaaaggg aggaaacggc acaaaagctt tagttagctc aatttccact ttgttatcta
    12541 ccgctcgaat ttccttgata aaggcttttg ctaacacacc cggtttgctt tttgcgatat
    12601 tcaaactatt taccactgct tgtgcggtaa gtacttgatc gttatggaat tttacccctt
    12661 cacgcaaggt aaaacgccag attgttccct cttcgttact gcgccagtca gtggccaatg
    12721 ccggaattaa attcccgtta agatcggctt cgaccaatgt ttccgctaag ttcatacgct
    12781 ggaaaattac gcctgattga ttcgggtcaa ggctattgat ttcccatggg gcaaccacgg
    12841 ttgcaagcgg taatatttcc gcattttttt gcgattgagt attctctact tgcggttttt
    12901 ctgcggttgc ttgagtgctg ttcttatcat cgcaagcaga aacacagaac gctgcgacag
    12961 ttaatggaat caaccatttt ttcattttat tttctccata aaaaaatcag ggctatgaat
    13021 attggcgaac tttagcatat ttccataaaa aaagtagagt tttctgcgag ggtatttttg
    13081 agtggttaat taataggcag aataatctca agataaggat tgttgatagg ggaatattta
    13141 ggctatttga ggaacagaat gcctagtatt gtaaaattcg gaaaaaaaat tgaccgcttg
    13201 tgctttattt gtaggggcga aagatttttc gcccctattg gttttagtgc tctctcgttt
    13261 taaagaactg tacgtcaggg taacgttctt gggcgaggtt taagttcacc attgtcggag
    13321 caatgtaggt gaggttgtct ccgccgtcta gggcaaggtt tgcctcgttt ttgcgtttga
    13381 actcttcaaa ttttttggca tctttgcctt cgacccaacg ggctgtggca acgtttacgg
    13441 tttcataaat tgcctctaca ccgtattcgg ttttcagccg tgaaaccacc acatcaaact
    13501 gtagcacccc aaccgctccc acgatcaaat cgttgttgat taacggacgg aatacttgca
    13561 ccgcaccctc ttcggaaagt tgtaccaagc ctttgagcag ttgtttctgt ttgagcggat
    13621 cacgcaggcg gattcggcgg aacagctcag gggcgaagtt cggaataccg gtgaatttga
    13681 aattttcgcc ttgggtgaaa gtatcaccaa tttggatcgt gccgtgattg tgcaagccga
    13741 taatatcgcc ggcgtaggct tcttcggctt gtgaacggtc gcccgccata aaggttaagg
    13801 catcggagat caccacatct ttgccgatac ggacgtgttt aagcttcatt cctttttcat
    13861 atttacctga aacgacacgc ataaaagcaa ctcgatctcg gtgtttcgga tccatattcg
    13921 cttgaatttt aaatacgaaa ccgctgaatt tttcctcgct tggtgaaact tcacgttcgt
    13981 ccgattgacg ggcttgtggt gccggtgccc attcggttaa accgtcaagg aaatgattga
    14041 ccccgaagtt acccagtgca gtgccaaaga aaaccggtgt cagctcgcct ttgataaatt
    14101 cggtgtgatc gaattcgtgc gaagcgcctt gtaccagctc taattcatca cgcaactgct
    14161 gggctaaatc atcgccaacg gcttgatcca gttccggatt atttaagcct ttaaccactc
    14221 gtacttcttg aatggtatga ccttgtccgg tttggtataa gtaggtttcg tccttgtaaa
    14281 ggtgatatac ccctttaaac aattttccgc aaccaatcgg ccaagtgatc ggcgcacatt
    14341 tgattttcag cacgctttcc acttcatcta acagctccat cgggtcacga atatcacggt
    14401 ctagcttgtt cataaaggtt aggattggcg tatcacgcaa gcgggtcact tccattaatt
    14461 taatggtgcg ttcctcaacc ccttttgccg agtcgataac cattaagcag ctatctaccg
    14521 cagtcaaagt acgataagta tcttccgaga agtcttcgtg ccccggtgta tcgagtaagt
    14581 ttaccaagca atcattgtac gggaactgca tcacggaggt ggtaatcgaa attccacgtt
    14641 gtttttccat ctccatccag tcggatttag cgtgttgtgc cgagcctttg ccttttaccg
    14701 agccggcagt ttggattgca tttccgtaga gcaatacttt ttcggtaatg gtggttttac
    14761 ccgcatcggg gtgggaaata atcgcaaagg tgcgacgttt attaacttct tgtgggtatg
    14821 acatttttct caaccaatta aaataaaaag ggcggaaagt ccgccaaaaa attaagcgtt
    14881 attttcgctg atttgaggcg agagggcaag cggttaaatt tggcggaaat tttgcaaaat
    14941 cggctagatt tgcaaaaatt ttgtaaaaat cgaccgcttg ctttggcaca attttggaaa
    15001 cagggtagaa taaccagctt tgactttatt atcgatgaat ctattatgat gaaaaaattt
    15061 attcctgtta tgattgcggc tctggtgtta agcgcctgtt cctcccagcc ggcaaaaagg
    15121 aaaagcacac cacggattaa agcaaccgct gcttatattc caaccgaaaa ttccgcttat
    15181 gtggtgtttg acttaaacca aaagaaattt ttggaaggta agaatttcaa tttagttcgc
    15241 ccgattgctt cggtcacaaa attaatgaca gcagtcgttt tcttagaaga aaaccgctat
    15301 ggggcgagtt gccaaaccca aattttgccg tcagatgctg atttcttaaa aggcacgacc
    15361 agcccattgc cgaaaaatct taacattgcc tgtagtgaat tgttaaaagc gatgctagtt
    15421 cgctccgata actatgccgc tcacgccctt gcccacgcaa cacatttgaa taaagcccaa
    15481 tttttggctc gaatgaacag caaagcccgt gaaatcggta tgactcaaac ctactttggt
    15541 gatagctcag gcttatcttc ggataatgtt tcaacggtgg ttgatcttgc taaattggca
    15601 ggctatgcgg caagtaagcc tgaaattcag caattgtcta attcgccatt agtgagtgtt
    15661 tatgcaggtg gtcgttattt ctctatgaaa aacaccaaca gtatggttcg ttcccaatcc
    15721 tatcaggcat tagtgagtaa gacaggttat acccgtgagg cgggttataa tcttgccttt
    15781 attgctaaag gggattgcag aggcaagcga attggtgtgg ttagtttaaa caatgataac
    15841 gctgcttcac gcaccgattt cactgaatcg atgttagcga agtatcaatg cgtaccaaat
    15901 ccgtatagac aatatgatta atggttgcct tgagaaaata gcggtttttc tctaaaatac
    15961 tgccttttaa attaaatgaa ttgagacaag attgtgtcag aaattaaatt aattgtcggg
    16021 ctggcgaacc cgggggtaaa atatgaagaa acccgccata atgcaggcga gtggctgatt
    16081 aacgaacttg cccgaatata taacgttagc ctaaaagaag agccgaaata ttttggcaaa
    16141 gtagcaaaaa ttaataccgc tcaaggcgaa gtccgtttat tagtgccgac tacttttatg
    16201 aatttaagtg gtaaagcagt gggagcgtta gccaattttt atcggattaa accggaagaa
    16261 attttggtcg ctcacgatga attggattta ccacccggtt cggtgaaatt gaaaaaaggc
    16321 ggcggacacg gtggtcataa cggcttgaaa gatattattg ccgcccttgg caataataaa
    16381 gagttttacc gtgtgcgaat tggtattgga cacccggggc ataaagactt agtggcgaat
    16441 tatgtgctgg gcaagccttc accaagcgat cagacattac tgaatagtgc ggttgatgaa
    16501 gccgcacgtt gcgtagaagt tttatttaaa gacggaatgg ttaaggcaac taaccgttta
    16561 aatagtttta aagcaaatta aaactctctc ccttgtggga gagagaccga aaaatacttc
    16621 gttcagaagt atttttcaga gagaggggag cggtcatatt tgaggaaatt attgcaattt
    16681 cgccctctcc ctcattttcg cttctgaacg aagctcaaat ttatccctct cccgcaaggg
    16741 gagaggggaa ttctaaggaa caaaaaatgg gatttaaatg tggtattgtg ggtttaccaa
    16801 atgtcggtaa atcgaccctt ttcaatgcat taactaaagc aggtattgag gcagcaaact
    16861 atccgttctg tacgattgag ccaaataccg gtgtggtacc aatgcctgac cctcgcttag
    16921 atgcgttggc ggaaattgtg aaacctgagc gagtgttacc aactacaatg gaatttgtgg
    16981 atattgccgg cttagtggca ggtgcaagca aaggcgaagg tttaggtaat aaattcttag
    17041 ccaatattcg tgaaactgat gcgattggcc acgttgtgcg ttgttttgaa aatgatgaca
    17101 tcgttcacgt tgcggggaaa attgacccag ctgatgatat tgaaatcatc aataccgaat
    17161 tggcacttgc cgatttagac agttgcgagc gtgccattca gcgtttacaa aaacgtgcga
    17221 aaggtggcga taaagacgca aaatttgaat tatcgattat ggaaaaaatc ctacctgtgc
    17281 ttgaaaatgc cggtatgatt cgttcggtag atttagataa ggatgaactg cacgctatta
    17341 agggctacaa cttcttaact ttaaaaccga caatgtatat tgccaacgtg aacgaagacg
    17401 gttttgaaaa taacccttat cttgaccgtg tgcgtgaaat tgccgaaaaa gaaggggcgg
    17461 tggttgtacc ggtgtgtgca gcgattgaat cggaaattgc cgagcttgat gacgaagaaa
    17521 aagtggaatt tctacaagat ttaggcattg aagagccggg cttaaaccgt gttattcgtg
    17581 caggctaccg tttacttaac ttacaaacct actttaccgc aggggtaaaa gaagtgcgtg
    17641 cgtggacggt ttcggtaggt gcaaccgctc caaaagcagc agccgtgatt cacactgact
    17701 ttgagaaagg ctttattcgt gcggaagtaa ttgcttacca agacttcata gacaataaag
    17761 gtgaagccgg tgcaaaagaa gcgggtaaat ggcgtttgga aggtaaagat tatattgttc
    17821 aagacggcga tgtaatgcac ttccgtttca atgtttaatt tgcaaatttt tgcctaaaaa
    17881 agaccgcttg ttgatctgtg atctacaagc ggcctttttt tgttaattaa ttgcatattg
    17941 ctcgattcgg taaaaatgtc ttaaataggg ctaactatta agtattaa
//
LOCUS       DANLUQ010000040        17897 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.040, whole genome shotgun sequence.
ACCESSION   DANLUQ010000040 DANLUQ010000000
VERSION     DANLUQ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 17897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17897
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.040"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..581
                     /locus_tag="PXB27_001055"
     CDS             <1..581
                     /locus_tag="PXB27_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345858.1"
                     /translation="IFTTDILERIANMPIIWKNFEENYLYNKKLDGLWSTNTNYIIDY
                     KLLNIPEEQPNVIIEFMVNKDDNSILGEMTSTALCELIPLTWSFRLESPKPTLLNLIF
                     DRELKLSYLVSGGYTELSTFKIKEINSNLGTLTLTTQEDKTGIFPPTIKLINFSSNIE
                     DDIKEIEQSCLNSRKQLWEKRRKMQEEQKIPN"
     gene            698..3562
                     /locus_tag="PXB27_001056"
     CDS             698..3562
                     /locus_tag="PXB27_001056"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012771641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDL5345859.1"
                     /translation="MTKQFTMEDRFNPSAVEQALYQHWESQGYFKPSEDINAPSYCIA
                     IPPPNVTGSLHMGHAFQQTLMDTLIRFNRMEGNNTLWQTGTDHAGIATQMVVERKIAA
                     EEGKTRHDYGREAFINKIWDWKAYSGGTISQQMRRLGNSIDWDRERFTMDEGLSNAVK
                     EVFVRLHEEGLIYRGKRLVNWDPKLHTAISDLEVENKESKGSLWHFRYPLANGAKTAD
                     GLDYLVVATTRPETMLGDTAVAVHPEDERYQALIGKSVILPLANREIPIIADEYVDRE
                     FGTGVVKITPAHDFNDYEVGKRHGLPMVNVMTLNADIRAEAEIIGTDGKPLSDYTAPI
                     PADYQGLERFAARKKIVADFEALGLLDQIKPHDLKVPYGDRGGVPIEPMLTDQWYVSV
                     KPLAEVATKAVEDGEIQFVPKQYENLYFSWMRDIQDWCISRQLWWGHRIPAWYDEQGN
                     VYVARDEAEVRAKHNLPADLPLKQDEDVLDTWFSSGLWTFSTLGWPEQTKELKMFHPT
                     DVLITGFDIIFFWVARMIMFTMHFIKDENGKPQVPFKTVYVTGLIRDEQGQKMSKSKG
                     NVLDPIDMIDGISLDDLLEKRTGNMMQPQLAEKIAKATRKEFAEGIVAHGTDALRFTL
                     TALASNGRDINWDMKRLEGYRNFCNKLWNASRYVLTNDKLDLSEGDVEFSLADRWIES
                     QFNRTVETFRTALSQYRFDLVANAIYEFTWDQFCDWYLELTKPIFFKGTDVQRRGASR
                     TLVNVLEKLLRLIHPVMPFITEEIWQKVKGFVGIEADTIMLQKFPQFDPLAIDETAES
                     QINFIKEVIVAVRNIRAESNIAPSKGLDLIARNFSADEVSILNANEVLLKSMAKLDSV
                     KVLENGENAPLSVAKLVANGEILIPMAGFINKEAELARLTKEMDKLKGEVARIEGKLS
                     NEAFVAKAPEQVIAKEREKMQEYLSGLEKLQVQYQEIEAL"
     gene            complement(3633..4226)
                     /locus_tag="PXB27_001057"
     CDS             complement(3633..4226)
                     /locus_tag="PXB27_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="HDL5345860.1"
                     /translation="MLTDQDIFNRYQTKKSEFEKQADISLIGHSLFDMWGDMENGTPN
                     LAGKTVANLGLSGTSTRQYLDVITRQERIQHVGDNVFIFLGVNDIVKEPDYSPKQVLE
                     WIGEIVDSLRKINPNANFFLLEATPVNNITTTNNAAIKELNAYFEANIPSDLTYVKTW
                     EYFADKEGNLDLTLCHDGLHFTQAGYDRLEAILTKHL"
     gene            complement(4361..5815)
                     /gene="gnd"
                     /locus_tag="PXB27_001058"
     CDS             complement(4361..5815)
                     /gene="gnd"
                     /locus_tag="PXB27_001058"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005714317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HDL5345861.1"
                     /translation="MSQKGDIGVIGLAVMGQNLILNMNDNGFKVVAYNRTTSKVDEFL
                     EGAAKGTNIIGAYSLEDLAAKLEKPRKVMLMVRAGEVVDQFIEALLPHLEEGDIIIDG
                     GNSNYPDTNRRVKALAEKGIRFIGSGVSGGEEGARHGPSIMPGGDESAWQYVKPIFQA
                     ISAKTDKGEPCCDWVGRDGAGHFVKMVHNGIEYGDMQLICEAYQFLKDGLGLSYDEMH
                     EIFKEWKNTELDSYLVDITTDILAYKDEDGEPLVEKILDTAGQKGTGKWTGINALDFG
                     IPLTLITESVFARCVSAFKDQRVAASRLFNKEIGKVEGDKKVWVEAVRRALLASKIIS
                     YAQGFMLIREASEQFGWDINYGNTALLWREGCIIRSRFLGNIRDAYEANPYLVFLGSD
                     PYFKDILENCLADWRKVVAKSVEIGLPVPCMASAITFLDGYTSARLPANLLQAQRDYF
                     GAHTYERTDKARGEFFHTNWTGRGGNTASTTYDV"
     gene            complement(5898..6587)
                     /locus_tag="PXB27_001059"
     CDS             complement(5898..6587)
                     /locus_tag="PXB27_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HDL5345862.1"
                     /translation="MKTLGILGGMSAESTVSYYLDINRSVNKVLGGNSSAKILISSVN
                     FEEIVQCQKQGNWQAAAEILSEQARLLEQAGAEGILLATNTMHKIADQISSHISVPFL
                     HILDCVAEAISAQGLQKVALLGTKFTMSDNFYKDGLLERGITSIVPDEETQQEIHRII
                     FEELCLGKITESAKAFYLQTIQQLSEQGAEGVILGCTEIGLLINQNDNPLPFFDTAAL
                     HSQMAVEFILG"
     gene            complement(6597..7484)
                     /locus_tag="PXB27_001060"
     CDS             complement(6597..7484)
                     /locus_tag="PXB27_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HDL5345863.1"
                     /translation="MNPIQLIEPNINSKIFTIRGEQVMLDVHLAELYQVDTRTFNQAV
                     KRNEERFPDIFRFQLTENEWLSLRSQFVILNENSGRGQHRKYLPYVFTEQGIAMLSAV
                     LRSEVAVQVSIKIMQAFVQMRHLLQTNSGLVQRLESVERKQLEMSGQLEQVFQSLEGN
                     KPKMQGVFFEGETFNAYQFVTELVRGAKSSLILIDNYVDDTVLTLFTKRSHNVSVTIY
                     TKQISKQLALDLEKYNSQYAHINVKIFDKSHERFLIIDEKELYHIGASLKDLGKKWFA
                     FSKMDLSMSRILEKLAENQ"
     gene            complement(7660..8358)
                     /gene="pgl"
                     /locus_tag="PXB27_001061"
     CDS             complement(7660..8358)
                     /gene="pgl"
                     /locus_tag="PXB27_001061"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HDL5345864.1"
                     /translation="MNKVIFPTAQAAVEQIAKEFIEYSHQNRPVHISLSGGSTPKLLF
                     KTLAQAPFNTEIQWQNLHFWWGDDRMVAPTDPESNYGEVQKLLFDHIDIPSENIHRIR
                     GEENVEQELARFSAELTACVPNLAFDWIILGMGTDGHTASLFPHQTDFNDQNVAVIAK
                     YPETGQIRISKTASLIEKAKRITYLVTGASKAEILKEIQTTPAENLPYPAAKIQAENG
                     VTEWYLDSEAANLL"
     gene            complement(8498..9985)
                     /locus_tag="PXB27_001062"
     CDS             complement(8498..9985)
                     /locus_tag="PXB27_001062"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDL5345865.1"
                     /translation="MNAENSCIVIFGASGDLTFRKLIPALYNLYKIGRLGEHFSVLGV
                     SRSELTDESFRQKMRDALVKFEKASGEELDKFCEHLYYQAVNTSDAVDYAKLLPRLDE
                     LHDKYQTAGNTLYYLSTPPSLYGVIPECLAAHGLTTEEFGWKRIIVEKPFGYDIETAK
                     KLDVQIHKCFEEHQIYRIDHYLGKETVQNLLVLRFSNGLFEPLWNRNFIDYVEITGAE
                     SIGVEDRGGYYDGSGAMRDMFQNHLLQVLAMVAMEPPAIINANSMRDEVAKVLYCLHP
                     LTEEDVKHNVVLGQYARGTVDGKEVPAYLEEKGVPADSNTETFMAVKCKIDNWRWAGV
                     PFYVRTGKRLPTRVTEIVIHFKTTPHPVFSQNAPENKLIIRVQPDEGISMRFGLKKPG
                     AGFEAKEVSMDFRYSDLSSSSSLLTAYERLLLDALKGDATLFARTDAVHACWKFVQPI
                     LAYKANRGRVYEYESGTWGPTEADKLIARHGKVWRKPSGTMKKKV"
     gene            complement(10060..10878)
                     /gene="cysQ"
                     /locus_tag="PXB27_001063"
     CDS             complement(10060..10878)
                     /gene="cysQ"
                     /locus_tag="PXB27_001063"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HDL5345866.1"
                     /translation="MQTLTQPLLENIINIAKAAGEQLKQLYARSVAIKIKADCTPVTE
                     ADLIISQFLTERLKQLTPDIPVLSEEFCDIPFAERQQWQEYWIIDPIDGTQQFINRTG
                     QFSVVIALVQKREGRNQPVLGVIHAPILGKTYFSMEGSGSFLQENGEIRPLVKPKRAE
                     NALLIVTVGSDLHAEQAKNAISAPQIECVKYGSGSLKAGLVAEGKADCYIRFGDTGEW
                     DTAVAEVLLSEMGGKIFDFNFSPLTYNQRESLINPYFVMVADGSIDWDKIFLFK"
     gene            complement(10892..11434)
                     /gene="nudE"
                     /locus_tag="PXB27_001064"
     CDS             complement(10892..11434)
                     /gene="nudE"
                     /locus_tag="PXB27_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HDL5345867.1"
                     /translation="MIKQLPEILNIKTVAKSRIFEVQAVDLRFSNGEERTYERLTPNR
                     RSSVMVIPIQNNELIFVKEYAVGSERYELGFPKGIVDAGEEPIESANRELQEEIGFGA
                     KKLDFLRSLYSSPSHMYGLMHLFLAQDLYPSKLEGDEPEPLEIVRYPLDKIDQLLADP
                     DFAEARNLSALFMLREYLKK"
     gene            11522..11938
                     /gene="hslR"
                     /locus_tag="PXB27_001065"
     CDS             11522..11938
                     /gene="hslR"
                     /locus_tag="PXB27_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HDL5345868.1"
                     /translation="MKNKHEKEQNDAEVRLDKWLWAARFYKTRSIAKAMIEGGKVHYN
                     GQRAKVSKAVEIGAIIKLRQGNEEKEVEVLALSDQRRGAPEAQLLYSETEKSIKHREA
                     MAFARKANALSMPHPDRRPNKKERRDLIKFKEQEFS"
     gene            12182..13075
                     /gene="hslO"
                     /locus_tag="PXB27_001066"
     CDS             12182..13075
                     /gene="hslO"
                     /locus_tag="PXB27_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDL5345869.1"
                     /translation="MNYTKDNDKLYRYLFKDRAVRGEWVRLNNTFTETLNTHHYPKAV
                     QNLLGEMLVATSLLTATMKFNGNITVQIQGDSPLKLAVVNGNDQQQLRALARVQADIA
                     DTASLAEMIGNGVLVISIIPTDGERYQGVIALDKPTIRECLEDYFARSEQLQTHLVIR
                     TGEFEGKAVAGGMLLQIMPDGTGSRDDFEHLATLTETVKDEELFGLEAEELLYRLYHE
                     EQVEVYPPQDTEFKCGCSRERSGSALLLVPAEEIEEMLEEKQGVIDMQCECCGTQYFF
                     DKAAIEGFKEETEKLSKLGLN"
     gene            complement(13149..13787)
                     /locus_tag="PXB27_001067"
     CDS             complement(13149..13787)
                     /locus_tag="PXB27_001067"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015431925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HDL5345870.1"
                     /translation="MQNKIDWQKVDGLLPVIVQNAQTCEVLMLGYMNQQALEKTLAEK
                     RVTFYSRTKQRLWTKGETSGNFLNVVDMSLDCDNDTLLILANPIGETCHTGAESCFHQ
                     FEQEQPDWIFFSKLERMIAERKNADPESSYTAKLYAKGTPKIVQKVGEEAVESVIAAM
                     KNDREELVGEVADLMYHLTVLLHNADLSWAEINAKLKERHKGIGLHPEGSNK"
     gene            complement(13815..14201)
                     /locus_tag="PXB27_001068"
     CDS             complement(13815..14201)
                     /locus_tag="PXB27_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL5345871.1"
                     /translation="MIKITALDHLVLTVADMQKTIDFYTTILGMQEITFGDNRKALLF
                     GTQKINLHQKGAEILPNAQNADCGTADLCLLTVTPLQEVIYVLQRHQVEILEGGIVPR
                     TGAVGKIESVYCRDPDGNLIEISLYV"
     gene            complement(14198..14971)
                     /gene="hisF"
                     /locus_tag="PXB27_001069"
     CDS             complement(14198..14971)
                     /gene="hisF"
                     /locus_tag="PXB27_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HDL5345872.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAQRYAQEGA
                     DELVFYDITASSDSRTVDKSWVERVAQVIDIPFCVAGGIKTIADAEQIFAFGADKISI
                     NSPALANPELITELAKRFGVQAVVVGIDSWFEAETGKYWVNQYTGDEKRTRQTEWQLL
                     DWVEEVQKRGAGEIVLNMMNQDGVRNGYDLVQLKKVREVCQVPLIASGGAGEMVHFRD
                     AFIEANVDGALAASVFHKQIINIGELKEYLAKEGVEVRR"
     gene            complement(14953..15702)
                     /gene="hisA"
                     /locus_tag="PXB27_001070"
     CDS             complement(14953..15702)
                     /gene="hisA"
                     /locus_tag="PXB27_001070"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006995165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HDL5345873.1"
                     /translation="MQKSIIIPALDLIDGQVVRLHQGDYAKQTTYADDPIAQFADYLA
                     QGAEQLHLVDLTGAKNPAQRQTALIGKIIRETHCKIQVGGGIRTEQDVADLLAVGANR
                     VVIGSTAVKEREMVKGWFKKYGAEKFVLALDVNIDAIGQKIIAMSGWQEASGVSLEEL
                     IEDYQTVGLQHVLCTDISRDGTLAGSNVDLYREICAKYPEIQFQSSGGIGSLADIKAL
                     CGTGVAGVIVGRALLEGKFNVAEAIECWQNG"
     gene            complement(15872..16717)
                     /locus_tag="PXB27_001071"
     CDS             complement(15872..16717)
                     /locus_tag="PXB27_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor protein"
                     /protein_id="HDL5345874.1"
                     /translation="MNEIKLFENSQIRSVWDSEKEEWFFSVVDIIEVLTQSSNPRRYW
                     SDLKRKIKDEEGGIELYEKIVQLKLKAPDGKMRETDATDLQGIFRIIQSVPSPKAEPL
                     KMWLAEVGKERIDEIIDPELTIDRALATYLKKGYSREWINQRLQAIQVRKELTDTWQD
                     HGVQVGREFAILTNEITQAWSGMTTRQYKDFKGLKKESLRDNMTTTELVLNMLAEAAT
                     KDIAQISHPQGLEENKTVAKRGGNVAKVARESLEAETGKPVITQQNAIDFAAVIEQVA
                     KQVKK"
     gene            complement(16816..17409)
                     /gene="hisH"
                     /locus_tag="PXB27_001072"
     CDS             complement(16816..17409)
                     /gene="hisH"
                     /locus_tag="PXB27_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015431920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HDL5345875.1"
                     /translation="MTNLTIIDTGCANLSSVKFAFDRLGIRAEISRDLNKIQSADKLL
                     LPGVGTAAAAMAILHDRRLIDTIRNATQPMLGICLGMQLMTDFSHEGDVATLNLMSGK
                     TERLPECGLPLPHMGWNKVQYQADHPLFHDIEQGNHFYFVHSYGVLPNENTIATCDYG
                     VPFSAAIASQNFYGVQFHPERSGEAGAKLLRNFVENV"
     gene            complement(17406..>17897)
                     /locus_tag="PXB27_001073"
     CDS             complement(17406..>17897)
                     /locus_tag="PXB27_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345876.1"
                     /translation="VILAFHLCDRDNSYDDFIGYINEFSFLEHIDLVYLESLMLSKSI
                     DNIGQDNIYKNILDLMFMNEVNLDDLISKLNPSRNGPVIILHETRTPELLECYKSILS
                     VKLKGEQLDIDLLNNNFKSDFFFNSLFLAIIKRFDKKAFEGNRYIESILLHYKKYLTQ
                     ETK"
BASE COUNT         5037 a         4085 c         3661 g         5114 t
ORIGIN      
        1 gtatcttcac aactgatatt ttagaacgca tcgcaaacat gcctattatt tggaaaaatt
       61 tcgaggagaa ttacctatat aataaaaaac tggatggttt atggtctaca aatacaaatt
      121 acattatcga ctataaatta ttgaacatac ctgaagagca accaaatgtt atcattgaat
      181 ttatggttaa taaagatgac aattcgattt taggggaaat gacttcaaca gctctgtgtg
      241 agttaatacc tctaacttgg agttttagat tggaaagccc taagccaaca ttattaaatc
      301 ttattttcga tagagagttg aaattgagtt atcttgttag tgggggatat accgagctat
      361 caacatttaa aattaaagaa attaacagta atctaggaac actaacatta actacgcaag
      421 aagataaaac cggtatattt cctccgacaa tcaaattaat caatttttca tccaatatag
      481 aagatgatat taaagagatt gagcaaagct gtttaaatag ccgaaagcaa ttatgggaaa
      541 aacgaagaaa aatgcaagag gaacaaaaaa ttcccaatta atcttttagt tgaccttaat
      601 ttaaataact aattacctat aaaagcattt acggcaaaga tcttttttat aagattaagc
      661 tagtgaaaaa attattaaac aagagaaaac aaaaccaatg acaaaacaat tcacaatgga
      721 agaccgtttt aacccttccg ctgtagagca agcgctctac caacactggg aatcgcaagg
      781 ctattttaag ccgagtgaag atattaatgc accgagctac tgtatcgcta ttccgccgcc
      841 gaatgtgacg gggagtttgc atatggggca cgctttccag caaaccttga tggatacgct
      901 gattcgtttt aaccgtatgg agggcaacaa taccctttgg caaacaggaa ctgaccacgc
      961 agggattgcc actcagatgg tggtggagcg taaaatcgca gcggaagaag gtaaaacccg
     1021 ccacgattat gggcgtgaag cctttatcaa caaaatctgg gattggaaag cctattcggg
     1081 cggcacgatc agccagcaaa tgcgtcgctt aggcaactcg attgactggg atcgtgagcg
     1141 ttttacgatg gacgaaggct tatccaatgc ggtaaaagag gtgttcgttc gcttgcacga
     1201 agaaggcttg atttaccgtg gcaaacgcct cgtaaactgg gatccgaaac tgcatactgc
     1261 gatttctgac ttggaagtgg aaaacaaaga aagcaaaggc tcgctgtggc atttccgcta
     1321 tccgctggct aatggtgcaa aaaccgcaga cggcttggat tacttagtgg tggcaacgac
     1381 ccgtcctgaa acgatgttgg gcgatactgc ggtggcggtt caccctgaag atgagcgtta
     1441 tcaagctctg attggcaaaa gcgtaatcct accgcttgcc aaccgtgaaa tcccgattat
     1501 tgccgatgag tatgtggatc gtgagttcgg tacgggcgtg gtaaaaatca cccctgccca
     1561 cgatttcaac gactacgaag tgggcaaacg ccacggcttg ccgatggtga atgtgatgac
     1621 cttaaatgcc gatattcgtg cagaggccga aattatcggc acagacggta aaccgctttc
     1681 cgactacacc gccccaattc ctgccgacta tcaaggctta gagcgttttg ccgcgcgtaa
     1741 gaaaattgtg gcagattttg aagctcttgg gctgttagat caaatcaaac cacacgactt
     1801 aaaagtgccg tatggcgacc gtggtggcgt gccgattgag ccgatgctga ccgaccaatg
     1861 gtatgtgtct gtcaaacctt tggcggaagt cgcgaccaaa gcggtggaag acggcgaaat
     1921 ccaattcgtg ccgaaacagt acgaaaacct gtatttctct tggatgcgtg atattcaaga
     1981 ctggtgtatc tctcgccaat tatggtgggg acaccgcatt ccagcgtggt atgacgagca
     2041 gggcaatgtt tatgtagcac gagatgaagc ggaagtgcgt gcgaaacaca acttacctgc
     2101 ggacttacca ttaaagcaag atgaagacgt actggatact tggttctcgt ctggtctatg
     2161 gacgttctca acgttaggct ggccagaaca aaccaaagaa ctgaaaatgt tccacccgac
     2221 cgatgtgctg attaccggtt ttgacatcat cttcttctgg gtggctcgta tgattatgtt
     2281 cacgatgcac ttcatcaaag atgaaaacgg caaaccgcaa gtgccgttca aaaccgtgta
     2341 tgtaacaggc ttgatccgtg acgagcaagg gcagaaaatg tcgaaatcga aagggaacgt
     2401 gttagacccg attgatatga ttgacggcat cagtctcgac gatttgctcg aaaaacgtac
     2461 aggtaatatg atgcagccac aactggcaga aaaaatcgct aaggcaaccc gcaaagaatt
     2521 tgccgaaggg attgtggcac acggcacaga cgctctgcgt ttcaccctca ccgcccttgc
     2581 cagcaacggt cgagacatca actgggatat gaaacgttta gaaggctacc gcaacttctg
     2641 caacaaactg tggaatgcga gccgttatgt gctgaccaat gacaagttgg atttaagcga
     2701 aggcgacgtg gaatttagcc ttgccgatcg ttggattgaa agccaattca accgcacggt
     2761 ggaaactttc cgcaccgcac tcagccaata ccgtttcgac ttagtggcaa atgcgatcta
     2821 cgaatttact tgggatcaat tctgcgactg gtatttagag ctgaccaaac ctattttctt
     2881 caaaggcaca gatgtacaac gccgtggcgc aagtcgcacg ctggtgaatg tattggaaaa
     2941 actcctgcgt ttaatccacc cagtaatgcc gttcatcacc gaagaaatct ggcagaaagt
     3001 gaaaggcttt gtaggtattg aagccgatac cattatgttg caaaaattcc cacaatttga
     3061 cccgcttgcc attgatgaaa ccgccgaaag tcaaatcaac tttatcaaag aagtgattgt
     3121 ggcggtgcgt aacattcgtg cggaaagcaa catcgcacca agcaaaggtt tagatttaat
     3181 cgctcgtaat ttctctgcgg acgaagtctc tattctcaac gctaacgaag tgttgctcaa
     3241 atcgatggca aaacttgata gcgtgaaagt gctggaaaat ggtgaaaatg caccgctatc
     3301 ggtagcgaaa ctggtcgcaa atggtgagat cttgatccca atggcaggct ttatcaacaa
     3361 agaagctgag ctagctcgcc tcaccaaaga gatggataaa cttaaaggtg aagtggctcg
     3421 cattgaaggc aaactttcaa acgaagcctt tgtcgccaaa gccccagagc aagtcatcgc
     3481 caaagagcgt gaaaaaatgc aagagtacct gagtggatta gagaaattgc aggtgcagta
     3541 tcaagagatt gaagcactgt aattaactaa caagcggtca ggatttggaa attttttgct
     3601 aattcagacc gcttgcttat tgaagctttt cattataaat gtttggttaa aatcgcctct
     3661 aggcggtcat agcctgcttg ggtgaagtgt aatccatcgt ggcataaagt tagatctaaa
     3721 ttcccttctt tgtcagcaaa atattcccac gtttttacat aagtcaaatc agatggaata
     3781 tttgcctcaa aatacgcatt gagctctttt attgcagcat tattggtggt ggtaatatta
     3841 ttaaccggtg tggcttccaa tagaaaaaag ttggcatttg gattaatttt gcgaagagaa
     3901 tccacaattt cgccaatcca ctctaacact tgttttggcg agtaatccgg ctcttttaca
     3961 atgtcattaa cgcctagaaa aataaataca ttatcgccaa cgtgttgaat tcgctcttgt
     4021 cgggtaatca catccaaata ttggcgggtg ctggtgcctg ataagcccaa attggcaact
     4081 gtttttcctg ctaaattcgg cgtgccgttt tccatatcgc cccacatatc aaacagggaa
     4141 tgcccaataa gggaaatatc tgcctgtttt tcaaattctg atttttttgt ttggtagcga
     4201 ttaaaaatat cttgatcggt aagcataggt tctccttaaa tataaatgaa aaatcgctcc
     4261 attattatac aaacttgtaa acattcactc caagatattt tgaagaaaaa tccaataaaa
     4321 aagccactta atatcgaatt aagtggctca aaaagattaa ttacacatca taagtggtcg
     4381 atgcggtatt accgccacgt cctgtccagt tggtgtggaa gaactcaccg cgagctttgt
     4441 ctgtacgctc ataagtatga gcaccaaagt agtcacgttg tgcttggagt aagtttgccg
     4501 gtaaacgtgc cgaggtgtag ccatctaaga aggtaatggc tgacgccata caaggtacag
     4561 gtaaaccgat ttccaccgat tttgccacta ctttacgcca atctgccaag cagttttcca
     4621 aaatatcttt gaagtatggg tctgagccta agaacactaa atatgggttg gcttcatacg
     4681 catcacgaat gttgcctaag aaacggctgc ggataataca accttcacgc cataataggg
     4741 cggtattgcc gtagttgatg tcccaaccga attgctcaga agcctcacga atcaacataa
     4801 agccttgtgc ataagagatg attttggaag caagtaacgc acggcgtacc gcttctaccc
     4861 atactttttt gtcgccttca actttaccaa tttctttgtt aaacaatctt gaagccgcaa
     4921 cacgttgatc tttaaacgca gatacgcaac gggcgaatac cgactcggta atcagtgtta
     4981 atggaatgcc gaaatcaagg gcgttaatac cagtccattt acccgttcct ttttgacctg
     5041 cggtgtcgag aattttctct accaatggct cgccgtcttc gtctttgtag gcaagaatat
     5101 cagtggtaat atccaccaaa tagctgtcta attcggtgtt tttccactct ttaaagattt
     5161 cgtgcatttc atcatagctt aaacctaaac catcttttaa gaattggtag gcttcacaaa
     5221 ttaactgcat atcgccatat tcgataccgt tgtgaaccat tttcacaaaa tgccctgctc
     5281 cgtcacgacc aacccagtca caacaaggct cacctttgtc ggttttggct gaaattgctt
     5341 ggaaaatcgg cttcacatat tgccacgcag attcatcacc gcccggcata attgacggcc
     5401 cgtgacgagc accctcttcg ccgcccgata cacctgagcc gataaagcga atgccttttt
     5461 ccgctaacgc tttcacacgg cggttggtgt ccggatagtt tgagttaccg ccatcaatga
     5521 tgatgtcgcc ttcttctaaa tgtggcagaa gagcctcgat aaactgatcc accacctcac
     5581 cggcacgcac cattagcatc actttacgcg gtttttccaa cttcgccgct aaatcttcta
     5641 acgagtaagc accgataatg ttcgtgcctt tcgccgcacc ttctaagaac tcatccactt
     5701 ttgaagtagt gcggttgtac gccaccactt taaagccgtt gtcattcata ttcaaaatta
     5761 aattttgacc cattacggcg agtccaatca cgccgatatc gcctttttgt gacattgttt
     5821 gctcctattg tttttgattc tatgcaagcg gtcgaattgg taaaattttt tgcaaatttg
     5881 accgcttgta acatttatta acctaaaata aactccaccg ccatttggct gtgtaatgct
     5941 gctgtatcga aaaacggtaa cggattgtcg ttttggttaa tcagcaaccc gatttcggta
     6001 caacctaaaa tcacaccttc tgcaccttgt tcactaagtt gttgaatagt ctgaagataa
     6061 aacgctttgg cactttcagt aatcttgcct aaacagagtt cctcgaaaat aatgcgatga
     6121 atttcttgtt gcgtttcctc atctggtact attgatgtga taccacgctc caataaaccg
     6181 tctttgtaaa aattatcact catagtaaat tttgtgccaa ggagtgctac tttctgcaga
     6241 ccttgagctg aaatcgcctc agccacacag tccaaaatat gcaaaaacgg cacagagata
     6301 tggctactaa tttgatcggc aattttgtgc attgtgttgg ttgccaataa aatgccttct
     6361 gctccggctt gttctagcaa tctagcttgt tcagataaaa tttcagcggc tgcttgccaa
     6421 tttccctgct tttggcattg tacaatctct tcaaaattga cactcgaaat taaaatcttg
     6481 gcactgctat ttccacctaa tactttattc accgagcgat taatatctaa ataataagaa
     6541 accgtgcttt ccgcactcat tccacctaaa atgccgagtg ttttcattaa tataccttat
     6601 tgattttccg ctaacttttc taaaatgcgg ctcattgata aatccatttt agaaaaagcg
     6661 aaccatttct tacctaaatc tttaagtgat gcaccgatat gataaagctc tttttcatca
     6721 ataatcaaaa atcgctcgtg agatttatca aatattttca cattaatgtg agcatattgg
     6781 ctgttatatt tttccaaatc taatgcaagt tgcttagata tttgtttcgt ataaatcgta
     6841 actgatacgt tatgcgatcg tttcgtaaat aaagttaaaa cggtatcatc tacatagtta
     6901 tcaattaaaa tcagcgatga ttttgcacct ctcactaatt ccgtaacaaa ctgataagcg
     6961 ttaaaggttt caccttcaaa gaaaacccct tgcattttag gcttattgcc ttcaagagat
     7021 tgaaacacct gctctaactg cccagacatc tccaactgtt tacgctctac actttctaag
     7081 cgttgtacca aaccactatt tgtttgcaaa agatgacgca tttgcacaaa ggcttgcata
     7141 atcttaatgc taacttgcac cgcaacttca ctacgcaata ctgccgaaag cattgcaatg
     7201 ccttgctcgg taaagacata aggaagatat ttcctatgtt ggccacgccc actattttcg
     7261 tttaagatca caaattgtga tcttaaagat agccattcgt tttctgttaa ttgaaaacga
     7321 aatatgtcag gaaatcgctc ttcattccgt tttactgctt ggttaaaggt tcgagtatct
     7381 acctgataaa gctctgccaa atgaacatct agcattactt gctcgccacg aatagtaaaa
     7441 attttagagt taatgtttgg ttcaattaat tgaattggat tcatctttct tccttattct
     7501 tcagtgaaga tcacttttgt gatctcaaaa taagaaagac tatgccaatt tttggcctat
     7561 accacgagca gatttttctg ctttttcgat gaatatcaca aaaatacttt gcaacatttt
     7621 tctaaaattt aaccgcttgt aaaggtcact gattgtgacc tagagtaagt tagccgcctc
     7681 actatccaaa taccattccg ttaccccatt ttccgcctga attttggcgg caggataagg
     7741 taaattttct gccggagtgg tttggatttc ttttaaaatt tccgctttgc ttgctcctgt
     7801 gaccaagtaa gtgatccgtt tggctttttc aattaaagat gccgttttgg aaatgcggat
     7861 ctgccctgtt tctgggtatt tggcgatcac ggcaacgttc tgatcattaa aatctgtttg
     7921 atgtgggaac agtgaggcgg tatgcccgtc tgttcccatt cctaaaataa tccaatcaaa
     7981 cgccaagttc ggcacacaag cggtcaattc tgccgaaaat cttgcaagtt cttgttctac
     8041 attctcttca ccacgaatgc ggtgaatatt ttcgcttgga atgtcgatgt gatcgaacag
     8101 taatttctgc acttcgccgt agttactttc gggatcggtt ggggcaacca ttcgatcatc
     8161 cccccaccag aaatgcaggt tttgccattg aatttcggta ttaaacggtg cttgagctaa
     8221 ggttttgaat aataatttgg gggttgaacc accggaaagg gaaatatgca ccgggcggtt
     8281 ttgatggctg tattcaataa actctttagc aatttgttca accgcagctt gggcggtggg
     8341 gaaaataact ttattcataa tttgcctcgt tagatcaccc ctctttagaa aagaggggag
     8401 ggggagattt gattacgatt tcaatatttc gtatgagcgt atataacttc tgccaaatct
     8461 cccctagccc ctctttgcta aagaggggaa ctgaaattta cactttcttc ttcattgtac
     8521 cggacggttt tcgccatact ttgccgtgtc tggcgattaa tttatctgcc tcggttggcc
     8581 cccaagtgcc ggattcatat tcatacacac gcccacgatt agctttgtag gctaaaatcg
     8641 gttgcacaaa tttccaacaa gcgtgtacag catcagtacg ggcaaataag gttgcatcac
     8701 ctttaagtgc gtctaatagt aagcgttcat aagcggtgag taagcttgat gatgaactta
     8761 aatcagaata gcggaaatcc attgagactt ctttcgcttc aaagcctgct cccggttttt
     8821 tcaagccgaa acgcattgaa atgccttcgt ccggttgcac acgaataatc aatttatttt
     8881 ccggagcatt ttggctaaat accgggtgtg gtgtagtttt aaaatgaatt acgatttccg
     8941 tcacacgggt tggtaagcgt ttaccggttc gcacatagaa cggcacacct gcccaacgcc
     9001 agttatcgat tttacatttt accgccataa aggtttcagt gttggaatca gccggcacgc
     9061 ctttttcttc caagtaagcc ggcacttctt tgccgtctac cgtgccacga gcatactgac
     9121 ctaacaccac attgtgttta acgtcttctt cagttaacgg atgcaagcaa tatagcactt
     9181 ttgcgacttc atcacgcatt gaattagcat taataattgc cggtggctcc attgcaacca
     9241 tggctaatac ttgcaataag tggttttgga acatatcacg cattgccccc gagccatcat
     9301 agtaaccgcc acgatcttca acgccgatgg attctgcccc ggtaatttcc acataatcaa
     9361 taaaattacg gttccaaagc ggttcaaata agccgttaga aaaacgtaaa acgagtaagt
     9421 tttgtaccgt ttctttacct aaatagtggt cgatacggta gatttgatgc tcttcaaagc
     9481 atttgtggat ttgtacgtct aattttttgg cggtttcgat atcgtagccg aatggttttt
     9541 caacaataat ccgtttccag ccaaactctt cggtagttaa gccgtgagcg gctaaacatt
     9601 ccggaatcac gccatataaa cttggtggcg tggagaggta ataaagcgtg ttaccggctg
     9661 tttgatattt atcgtgtagc tcatcaagac gaggcagcaa cttggcataa tccactgcat
     9721 cagaggtatt tacagcttgg taataaagat gttcgcaaaa tttatctaat tcttcaccgc
     9781 ttgctttttc aaatttgact aacgcatcac gcattttttg gcggaaagat tcgtcggtta
     9841 attctgaacg agaaacacct aggacggaaa aatgctcgcc caatctgcca atcttatata
     9901 agttataaag agcaggaatt aatttacgaa aagttaagtc accggaagca ccaaaaatga
     9961 cgatacagct attttcagca ttcatttatt tatcctattg ctaattgtta aaattttgtt
    10021 gggtgcaaga ttatcataaa aattgctaat cggaactacc tatttgaata aaaatatttt
    10081 atcccaatct atcgagccgt cagccaccat cacaaaataa ggattaatca aactttctcg
    10141 ctgattataa gtcagcggcg aaaaattaaa gtcgaaaatt ttgccgccca tttcacttaa
    10201 cagcacttct gccacagcag tatcccattc gccggtgtca ccaaaacgga tataacaatc
    10261 ggctttccct tctgccacta aaccggcttt tagactgccc gaaccatatt ttacacactc
    10321 aatttgtggg gctgaaatgg catttttagc ttgttctgca tgtaaatcgc tgcctacggt
    10381 aacaatcaaa agggcattct ccgcacgttt cggtttgaca agcggtcgaa tttctccatt
    10441 ttcttgcaaa aagctaccgc taccttccat actaaaatag gtttttccta atatcggagc
    10501 atgaataacg cccagcaccg gctgatttct accctcacgt ttttgcacta acgcaatcac
    10561 cactgaaaat tgaccggtgc ggttaataaa ctgttgtgtg ccgtcaatcg ggtcgataat
    10621 ccaatattct tgccattgtt ggcgttcggc aaacggaata tcgcaaaact cttccgataa
    10681 taccggaata tctggtgtga gttgcttcaa gcgttcagtt aaaaattggc tgataatcag
    10741 atccgcttcc gtcaccggtg tgcaatcggc tttgatttta attgccaccg agcgggcata
    10801 aagctgcttt aactgctcac cggctgcttt ggcaatattg ataatattct cgagtagggg
    10861 ttgagttagc gtttgcataa tagctccttg cctatttttt taagtattct cgtagcataa
    10921 atagagcgga caaattccgt gcttcggcaa aatcaggatc tgccaataat tgatcaattt
    10981 tatccaacgg ataacgcaca atttccagcg gttcaggctc atcgccttcc aattttgatg
    11041 ggtacagatc ttgtgccaaa aacaggtgca ttaagccgta catatggctt ggcgaactgt
    11101 aaagcgaccg cagaaaatct aattttttcg ccccaaaacc aatttcttct tgtaattcac
    11161 ggttcgcact ctcaatcggc tcttcacctg catccacaat gcctttcgga aaacccagct
    11221 cgtaacgctc cgagccgacc gcatactctt tcacaaaaat cagctcgttg ttttgaatcg
    11281 gaatcaccat taccgaagaa cggcgattcg gcgtaagtcg ttcgtaagtt cgctcttcgc
    11341 cattagaaaa gcgtaaatcg accgcttgca cctcaaaaat gcgagatttc gctactgttt
    11401 taatgttcaa aatttcagga agttgtttta tcattttatc tcaccttttt gagttatttt
    11461 agttgaaaaa tctcactttc gctctaatat ttcgactatt gttttgattt taaggcagaa
    11521 aatgaaaaat aaacacgaaa aagaacaaaa cgacgccgaa gttcggctag ataaatggct
    11581 ttgggcggct cgtttttata aaacccgttc gattgccaaa gcaatgattg aagggggtaa
    11641 agtccattat aatgggcaaa gagctaaagt tagcaaagcg gtggagatcg gtgccatcat
    11701 caaacttcgc caaggcaatg aagaaaaaga ggtggaagtg ttagccctca gtgaccaacg
    11761 ccgaggggct ccggaagccc aactgctcta cagtgaaacc gaaaaaagca ttaaacaccg
    11821 tgaggcaatg gcatttgccc gcaaagcgaa tgctctttca atgccacacc ctgatcgccg
    11881 tccaaacaaa aaagaacggc gggatttaat caaattcaaa gagcaagaat ttagctaagc
    11941 ccttgcaatc agaaaaataa tccctatatt gaatatccta agtaggggcg aaaaattttt
    12001 cgcccctata taattatttc gggcgaacat tgattcgccc ctacagcgtt cgatttgcac
    12061 atttttagcc acaaaatgca atgcattttg aacgttggct ttgccaacgg ctccgtagga
    12121 gcgaacaaaa ttagaaataa ttttgtgagt aataatcgac cgcttgcaac gaggaatcaa
    12181 aatgaattac accaaagaca atgacaaact ctaccgctat ttattcaaag accgagcggt
    12241 acggggcgaa tgggtgcgtt taaacaacac ctttaccgag accttaaaca cccatcacta
    12301 ccctaaagca gtacaaaatt tactcggaga aatgttagtc gcaaccagtt tgctgactgc
    12361 aacaatgaaa tttaacggca acattactgt gcagattcaa ggtgacagcc cgttaaaatt
    12421 ggcggttgtg aacggtaacg accaacagca actccgtgct ttagcccgtg ttcaggcaga
    12481 tattgcagac acagcctcgt tagcggaaat gatcggcaat ggtgtattag tgatttctat
    12541 tatcccaacc gatggtgaac gttatcaagg ggtgattgct ctcgataaac caaccattcg
    12601 tgaatgttta gaagattact tcgcccgctc cgagcaatta caaacccacc tcgttatccg
    12661 cacaggcgaa tttgaaggca aagcagtggc aggcggtatg ttgctacaaa ttatgccgga
    12721 cggcacaggc tcaagagatg attttgagca tttagccacg ctcactgaaa ccgtaaaaga
    12781 tgaagagtta tttggtttag aggcagaaga attgctctat cgcctctacc acgaagagca
    12841 agtagaagtt tacccaccac aagatactga atttaaatgt ggctgctcaa gagagcgttc
    12901 aggctccgca ttattactgg tgccggcaga agaaatcgaa gaaatgttgg aggaaaaaca
    12961 aggcgtgatc gatatgcaat gtgaatgctg cgggacacaa tatttctttg acaaagcggc
    13021 gattgaaggc tttaaggaag aaactgaaaa attaagtaag ttagggttaa attaagctaa
    13081 acaagcggtc aagtttcttg tcttttttgc aatttgtaaa aaaacggtga aatttgaccg
    13141 cttgcatttt acttatttga gccttctggg tgtaacccaa tccctttgtg gcgttctttt
    13201 agtttagcat tgatctctgc ccacgagaga tcggcattgt gcaataacac cgttaagtga
    13261 tacatcagat cagccacttc accgaccaac tcttcccgat catttttcat tgccgcaatc
    13321 acgctttcta ctgcctcttc ccctactttt tggacgattt ttggcgtgcc tttggcgtaa
    13381 agtttagcgg tataagaact ttccggatca gcatttttac gttcggctat cattcgttct
    13441 aatttgctga aaaagatcca atctggttgt tcttgttcaa actgatgaaa acagctctcc
    13501 gcccccgtat ggcaggtttc gccgataggg ttagcgagga ttaacagggt gtcgttatca
    13561 caatccaaac tcatatccac cacgtttagg aaattgcccg aggtttcgcc tttcgtccac
    13621 agccgttgtt tagtacggga gtaaaaagtc actcgctttt cagccagcgt tttttcgagg
    13681 gcttgttggt tcatatatcc taacatcagt acttcgcagg tttgggcgtt ttggacgata
    13741 acgggcagta agccgtccac tttttgccag tctattttat tttgcatttt ttcttccttt
    13801 ttttgaccgc ttgtttagac atataggctt atttcaatca aattaccatc agggtcacgg
    13861 caataaaccg actcaatttt tcccactgcc cccgtacgag gcacaatccc accttccaaa
    13921 atctccacct gatgtcgctg taacacatag ataacctcct gaagtggagt taccgttaag
    13981 aggcataaat ctgccgtgcc acaatcggca ttttgggcat tgggtaggat ttctgcacct
    14041 ttttggtgga ggttgatttt ctgtgtgcca aacaaaagcg ctttgcgatt atcgccaaag
    14101 gtgatttctt gcatacccaa aatggtcgta taaaaatcaa tggttttttg catatcggca
    14161 acggttaaaa ccagatgatc taaggcagta atcttaatca tctacgcacc tcaacccctt
    14221 ccttcgccaa atattccttc aactccccaa tattgataat ttgtttatga aacacactcg
    14281 ctgccaacgc accatccaca tttgcctcaa taaaagcatc acggaaatgc accatttcgc
    14341 ctgcaccgcc tgaggcgatg agtgggactt ggcaaacttc acgcactttt ttcagttgca
    14401 ccaaatcgta gccgttgcgt acgccgtctt ggttcatcat attcagcacg atttcgcctg
    14461 ctcctcgctt ttgcacttcc tccacccaat ccaacaattg ccattcggtt tggcgggtgc
    14521 gtttttcatc gccggtatat tgattgaccc aatatttgcc ggtttctgcc tcgaaccagc
    14581 tatcaatacc aaccaccacc gcctgcacgc caaagcgctt tgccagttcg gtaatcagct
    14641 cggggttggc aagggctggg gaattgatgg agattttgtc cgctccgaaa gcgaaaatct
    14701 gctccgcatc ggcaatggtt ttgattccgc ctgccacaca gaacggaata tcaatcacct
    14761 gtgccacacg ttccacccag cttttatcca ccgttctgct gtcacttgag gcggtaatat
    14821 cgtaaaacac caactcatcc gccccctctt gggcataacg ctgggcgagt ggcacaatgt
    14881 cgccgatgat ttcgtggttg cggaactgca cgcctttcac aacttgcccg tcacgcacgt
    14941 ctaaacaagg aattatccgt tttgccaaca ttcgattgcc tccgctacat taaatttacc
    15001 ttccaacaac gcacgaccga caatcacgcc agccacgccc gtaccacaaa gcgctttgat
    15061 gtcggcaagc gagccgatac cgcctgagga ctgaaattga atctctgggt atttggcaca
    15121 gatttctcga tagagatcta catttgaacc tgccagcgtg ccgtctcggg aaatgtcggt
    15181 gcataaaacg tgttgcaagc ccacggtttg ataatcttca atcaactcct cgagcgacac
    15241 accacttgcc tcttgccagc cgctcatcgc aatgattttt tgaccgattg catcaatgtt
    15301 cacatccagt gccagcacaa atttctccgc accgtacttt ttgaaccagc ctttaaccat
    15361 ttcacgctct tttaccgcag tcgaaccaat caccacacgg tttgctccaa cagcaagtaa
    15421 atccgccaca tcctgctcgg tgcggatacc gccgcccact tgaattttgc agtgggtttc
    15481 acgaataatt ttgccgataa gtgcggtctg tctttgagcc ggatttttcg cccctgtaag
    15541 atcgaccaaa tgtaactgct ccgccccttg tgccaagtag tcggcaaatt gggcaatcgg
    15601 gtcatcggca taagtcgtct gtttcgcata atcgccctga tgtaggcgaa ctacttgccc
    15661 atcgataagg tcgagggcgg ggataatgat ggatttttgc atattattgt tcctgtgtgc
    15721 ctagcacgag ccgtgctagg ctgaataatc ccagtcgcgc tgcgactgcc tctctaagct
    15781 acggagtagc ttggtttatc tagccccata cggctcgtat ggggtacaag cggtcaaatt
    15841 cttccaaaaa cttgcaaaaa accgaccgca cttatttctt cacctgtttc gccacttgtt
    15901 caatcaccgc agcgaaatca atcgcatttt gttgcgtaat caccggtttg ccggtctctg
    15961 cctccaaact ttcccgagcc acttttgcaa catttccgcc ccgtttggca acggttttat
    16021 tttcttccaa gccctgtggg tggctgattt gagcgatgtc tttggtggca gcctcagcaa
    16081 gcatattcag caccaactcg gtggtcgtca tattgtctcg cagactttcc tttttcagcc
    16141 ctttgaaatc cttatattgg cgagtagtca taccgctcca cgcctgtgtg atttcattgg
    16201 tgagaatcgc aaattctctg cccacttgca caccgtgatc ttgccaagta tccgtcagtt
    16261 cttttcgcac ctgaatggct tgcaagcgtt ggttaatcca ctcacgggaa tatccctttt
    16321 tcaagtaagt agccaaagct cggtcaatgg tcaattcagg atcgataatc tcatcaatcc
    16381 gctctttgcc tacctctgcc agccacattt tcaacggctc ggctttcggg cttggcacag
    16441 actgaataat acggaaaatg ccttgtaaat ccgtggcatc ggtttcccgc atttttccgt
    16501 ctggggcttt taatttcaac tgtacgattt tttcgtacag ttcaattccg ccctcttcgt
    16561 ccttgatttt ccgctttaaa tcactccaat aacggcgtgg attgctactt tgcgtcagca
    16621 cctcgataat gtccaccacc gagaaaaacc actcttcttt ttcgctatcc cacaccgagc
    16681 ggatttggct gttttcaaat agtttaattt cgttcatttc tcaccttctt tcactggtta
    16741 aattaacagt aattttgtcg aaataaagcg agaaatcaag ccctattttc aaacttttcg
    16801 atcgagatca caaaatcaga cattctccac aaaattcctg agcaacttcg ccccagcctc
    16861 gcccgaacgc tcggggtgga actgtacgcc gtagaagttt tggctggcaa tggcagcgga
    16921 aaacggcacg ccgtagtcgc aggtggcgat ggtgttctca ttcggtagca caccgtagct
    16981 atgcacaaaa tagaaatgat tgccttgctc aatgtcgtga aatagcggat ggtcggcttg
    17041 gtattgcacc ttgttccagc ccatatgcgg caacggcaaa ccgcattcag gcaagcgttc
    17101 ggttttgcca ctcattaagt tcagtgttgc cacatcgcct tcgtgggaaa agtccgtcat
    17161 taactgcatt cctaagcaga tgccaagcat cggctgggtg gcgttgcgaa tggtgtcgat
    17221 aagtcggcga tcgtgcaaga tcgccatcgc cgccgctgct gttcccacac cggggagtag
    17281 caatttatcg gccgattgga ttttgttgag atctcgcgag atctcagccc gaatgccaag
    17341 acggtcaaaa gcaaatttga ccgaagacag gttggcacag cctgtgtcga tgatggtgag
    17401 gttggtcatt tagtttcctg agttaagtat ttcttatagt gaagtaaaat tgactcaata
    17461 tatctgtttc cttcaaaagc tttcttatcg aatcttttga taattgccaa aaataaacta
    17521 ttaaagaaaa aatcagattt aaaattatta tttagtaaat ctatatctag ttgctcacct
    17581 tttagtttta cacttaaaat agatttataa cattccaata actctggagt tcttgtttca
    17641 tgcaatataa ttacaggtcc attcctagaa ggattcaact tggaaattaa gtcatctaaa
    17701 tttacttcat tcataaacat cagatctaaa atatttttat aaatgttatc ttgaccaatg
    17761 ttatctattg acttagaaag cattaaagac tctaagtaga ctaaatctat atgttctaaa
    17821 aaactaaatt catttatata acctataaaa tcatcatatg aattatctct atcacaaaga
    17881 tgaaaggcta aaataac
//
LOCUS       DANLUQ010000041        17681 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.041, whole genome shotgun sequence.
ACCESSION   DANLUQ010000041 DANLUQ010000000
VERSION     DANLUQ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 17681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17681
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.041"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            124..1257
                     /gene="carA"
                     /locus_tag="PXB27_001074"
     CDS             124..1257
                     /gene="carA"
                     /locus_tag="PXB27_001074"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDL5345877.1"
                     /translation="MFKPAILVLTDGSVFSGKSIGADGHTIGEVVFNTAMTGYQEILT
                     DPSYTKQIVTLTYPHIGNTGINLEDKESPNVCASGLIIRDLPLLHSNFRASSSLSDYL
                     IQQNIVAIADIDTRRLTRILREKGAQAGCILVGDATEENIANALAFANSFGSMAGKDL
                     AQSVTCNELYKWTDGEWQLGKGYVPQQQTEFHVVAYDYGVKHNILRMLAERGCRLTVV
                     PAKTSAEAVLALNPDGIFLSNGPGDPEPCDYAITAIRKLLETQKPIFGICLGHQLLGL
                     AAGGKTKKMVFGHHGANHPVQDLDSQKVLITSQNHGFEVDESSLPSNVRVTHRSLFDG
                     TVQGIELTDRPAFSFQGHPEASPGPNDVAYLFDRFVSEMKKVK"
     gene            1360..4566
                     /gene="carB"
                     /locus_tag="PXB27_001075"
     CDS             1360..4566
                     /gene="carB"
                     /locus_tag="PXB27_001075"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDL5345878.1"
                     /translation="MPKRTDINTILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     VLVNSNPATIMTDPNMADVTYIEPIEWKTVEKIIEKERPDAILPTMGGQTALNCALDL
                     SKNGVLKKYNVELIGATEDAIDKAEDRGRFKEAMTKIGLNTPKSFVCHSFEEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNRDEFIAICERGFEASPTHELLIEQSVLGWKEYEM
                     EVVRDRADNCIIVCSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAINPANGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLNE
                     LRNDITGGLIPASFEPTLDYVVTKIPRFAFEKFANADDRLTTQMKSVGEVMAMGRTFQ
                     ESLQKALRGLETGICGFNLLSEEPEKIRRELANPGPNRILYVADAFGAGFSLEEVHHY
                     SKIDPWFLVQIQDLVLEELALEKKQFSDLDANEIRRLKRKGFSDKRIAQLTKVSEKMV
                     RDYRHTYAILPVYKRVDTCAAEFASDTAYLYSTYEEECEAKPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHASLALREAGYETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RVENPWGVIVHYGGQTPLKLANALSDAGVNIIGTSADSIDAAEDRERFQQILNTLGLK
                     QPANRTARNALEAVALANEVGYPLVVRPSYVLGGRAMQIVYNDEELNRYMREAVSVSN
                     DSPILLDHFLNNAIEVDVDCICDGENVLIGGIMQHVEQAGIHSGDSACSLPAYSLSGH
                     VLDEIRKQTNAMAFALGVKGLMNVQFAVQNEVVYVLEVNPRASRTVPFVSKATGRPLA
                     KIAARVMAGETLADLNALEEIIPSKFFVKEAVFPFIKFPGVDTILGPEMRSTGEVMGI
                     GNTFAEAFFKAQLGAGERIPKVGKVFMSVIEEHKPALIYIAKRFQEQGYGLCATFGTA
                     NFLRQHGIAVQTVNKVREGRPHIVDAIKNGEIALVINTVSGEPEVIADSHSIRRTALQ
                     QRVPIYTTLAGADAISYGVKHINEFDVYSVQELEQKAN"
     gene            complement(4641..5432)
                     /locus_tag="PXB27_001076"
                     /pseudo
     CDS             complement(4641..5432)
                     /locus_tag="PXB27_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012072249.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
     gene            5635..6633
                     /gene="yiaK"
                     /locus_tag="PXB27_001077"
     CDS             5635..6633
                     /gene="yiaK"
                     /locus_tag="PXB27_001077"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005757429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HDL5345879.1"
                     /translation="MRVSYEELKAQFKRVLLARNVREEIAEECATVFADTTQAGAYSH
                     GVNRFPRFIQQLENGDIVPEAVPTKVLSLGAIEQWDAHQAIGNLTAKKMMDRAMELAS
                     ENGIGVVALRNANHWMRGGSYGWQAAEKGYIGICWTNALAVMPPWGAKECRIGTNPLI
                     IAVPTNPITMVDMSCSMYSYGMLEVHRLAGRQTFVDAGFDDDGNPTRDPATVEKNLRL
                     MPMGFWKGSGLSIVLDMIATLLSNGESTAAVTEDKDDEYCVSQVYIAIEVDRLIDGKT
                     KDEKLNRIMEYVKTAERADPDVAVRLPGHEFTQILADNKANGIPVDDTVWAKLQAL"
     gene            6758..7123
                     /locus_tag="PXB27_001078"
     CDS             6758..7123
                     /locus_tag="PXB27_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDL5345880.1"
                     /translation="MFFGHISQVKANRYPPAIQIALNYLKNTDFDAMEAGVYELKGRQ
                     IYVQVLDLNTKSKHEFQPEVHRNYLDVQYLHRGKEIMAAAVDTGTNPIAMEYNPERDI
                     QYYQSVANENEFRCVEGNF"
     gene            7187..7666
                     /locus_tag="PXB27_001079"
     CDS             7187..7666
                     /locus_tag="PXB27_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDL5345881.1"
                     /translation="MKALAQLVGKLLEILVVVILSAMSILVFVNVVLRYGFNSSISVT
                     EEVSRYMFVWLAFLGAVLAFSENQHVKVTMLTDKLSPTARNILSVVTDLAMLFCCYLI
                     VDGSWIQFNLNLNNFAPISGLPQGITFLASTVAGVLIGLLIIARIVATVGVIAKGEK"
     gene            7667..8944
                     /locus_tag="PXB27_001080"
     CDS             7667..8944
                     /locus_tag="PXB27_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017806296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HDL5345882.1"
                     /translation="MTVVIFLSVLLGSILLGIPVAFALLVCGVALMLHLDLFDAQILA
                     QQIVSGADSFSLMAIPFFILAGEIMNEGGLSKRIIDLPMKLVGHKRGGLGFVAILAAM
                     IMASLSGSAVADTAAVVAMLLPMMKTTGYPIDRSAGLIGTAGIIAPIIPPSIPFIVFG
                     VASGVSITKLFLAGIFPGIMMGICLAALWWWQAKRLDLMTFSKATKEELCISFKNSIW
                     ALLLPVIIIGGFRSGMFTPTEAGAVATFYALIVSLFVYREMKFKDLYKVVLAAAKTTA
                     VVMFLVAAANVTGWLITVAELPMMLTELLEPLIESPTTLLLVIMLAVFIIGMVMDLTP
                     TVLILTPVLMPLVEEAGIDPVYFGVLFILNTSIGLITPPVGNVLNVITGVSKLPFDQA
                     AKGVMPYLVMMICLLLLFVFFPSLVLVPLSWMQ"
     gene            9047..10033
                     /locus_tag="PXB27_001081"
     CDS             9047..10033
                     /locus_tag="PXB27_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDL5345883.1"
                     /translation="MKLFNLKTLFALVAGVAVMSGSAMAETSLRFGYEAPRSDTQHIA
                     AKKFDELLAAKTKGEIKLKLFPDSTLGNAQTMISGVRGGTIDIEMSGSPNFTGLEPKL
                     NVIDIPFIFKDREHVYKVLDGEVGQGLLKDLEAQGLKGLAFWDVGFRAFSNSKHPVTK
                     PEDIKGLKVRTNQNPMYIQAFTLLGGNPVPMPLSELYTALETRAVDAQEHPIGIFWSA
                     KLFEVQKHLSLTNHGYTPLIVVMNKAKFDGLAPELQTALVEAAKEAGQFQRDLNVKNE
                     KEIIEKLRKQGIEVIEQVDNAPFKAAIEAEVRKAFVEKHGDELLKKVDALAQ"
     gene            10107..11090
                     /locus_tag="PXB27_001082"
     CDS             10107..11090
                     /locus_tag="PXB27_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDL5345884.1"
                     /translation="MKLFNLKTLAAFVAGFALFSTAQAETSFRFAYEAPRSDTQHIAA
                     KKFNDLLQEKSQKGLKLSLFPDSTLGNAQTAISGVRGGTIDIAMSSSSNFTGLEPKLN
                     VIDIPFIFKDRDHAYKALDGEIGQGLLTALEAHGLKGLAFWEVGFRAFSNSKHPITKP
                     EDIKGLKIRTNQNPMYIQAFSILGANPVPMPLSKLYTALETKAVDAQEHPIGIFWSAK
                     LYEVQKYFSFTNHGYTPLIVAINKAKFDALPAEQQTALVEAAKEAGKYQRQLNLENEQ
                     KIIDSLKKAGIEFVDNLDTTPFKDAVSAETRKAFIEKNGDGLVKAIDALAK"
     gene            11221..12705
                     /locus_tag="PXB27_001083"
     CDS             11221..12705
                     /locus_tag="PXB27_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL5345885.1"
                     /translation="MQHIIKRFGSHTAVNDVSLDIHSGEIVALLGENGAGKSTLIKIL
                     AGIYDRDEGDILFHNQKIQSAHAIEKGNKKPIAFIHQDLGLIEWMTIAENMAFVMGFP
                     RKFGLIDWAKVREQSQKALDFVGIKLSADTRVFDLSRTEKALLAIARAIAVNAEILVL
                     DEPTASLPASDVEHLFTVLNRLRSQGVGMIYVTHRLDEVIAISDRILVMRDGFPVAEG
                     QTKLYDVKGLVKAIVGEESRGRQRVDLPTDTPEILSLQNVVVGDTGPVNFRLHKGEMI
                     ALAGLRGAGQEEIGRLLFGERTLDKGEIQLDKRSYSAKDPQDAIAKGVALVAGDRTRE
                     SVFMSMTTSENLFINPVLSGHSPLKRYSRREEWGESWYKFQLFDIRPKNLFIDASALS
                     GGNQQKIVLARWMHLNTPVLILEDPTAGVDVGARAEIYDLLNKALAEGVAIIVISTDF
                     EEIAHLCNRALVFNRGEIAGELLNEEVTFANLLALASDSQSKKQ"
     gene            12900..13946
                     /locus_tag="PXB27_001084"
     CDS             12900..13946
                     /locus_tag="PXB27_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5345886.1"
                     /translation="MAQTNIKSTALEQPVSIARDGFMAWFSQMSTRYGLLWLCVLLVI
                     IFSLTTESFASMLTLNAILESKSKIALLALAATTTMIVGKIDLNVGFGIVLWHILVIT
                     LQVQYGFSWYMAILIVLVIAALYGLLNGILVALADIDSFVATLGSGTVLYAVALWHSG
                     GRQIVGDLPDEFIALNSTEIFGIPISAFYVLAVAIVMWLVTEHTPTGRCMYAVGGNPT
                     AARLNGINIKKYTIVPFIVSSVITAFTGVLISAQQGVGQASVGMDYLLPALVGAFLGS
                     TTIRPGRINVWGTVVGIAILAIGISGIQQFGGAFWVEPLFNGATLLLSITIAGYAQRK
                     RLLNQKAVQKKSTN"
     gene            13959..15068
                     /locus_tag="PXB27_001085"
     CDS             13959..15068
                     /locus_tag="PXB27_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005757420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL5345887.1"
                     /translation="MKKAFRTTLTAIATLTAIAFGNNAFANDDPFVQEAKQLVAAATA
                     KQEKWDGPTTGPQLQKEKTVIFIASDMKNGGVLGVIDGMKEAVGVTGWKLDVLDGAGT
                     VNNQLAALNQAIARKPDAIVIGGWNPNVAKIPLQKATKNGIKLAAWHATPEPGAIDKY
                     GIFYNVTSDSDEIARLSAMLAVAESNGKAKAIILTDSLYEIALRKANVMKEVIEKCKE
                     CKVLEFIDTPLADTSSRMPNLTFSLLQKYGDDLEYALAINDLYFDFMAPSLRTAGKDK
                     PFSISAGDGSVTAYQRIRNGDKQFATVPEPLNLHGWQLVDELNRAFANEKPSGYITPA
                     HLVTKENVAFDGGENNLYAPQNGYKGAYKAIWGVK"
     gene            15173..16063
                     /locus_tag="PXB27_001086"
     CDS             15173..16063
                     /locus_tag="PXB27_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HDL5345888.1"
                     /translation="MSNKFIPKISNPRFNELVGNTFELEELFDKAIWAEGPAWSKPQQ
                     ALYFSDVKGCTMYRWTEQDGTQVFRQPSYFSNGNAFDANDNLITCEHGNRGISLTTPS
                     GEYRFLVGQLDGKRLNSPNDVVVKSDGIIWFTDPPYGILSDNEGKQSPSEIIGCYIYC
                     YDPKTDKLNIATFNTMRPNGLAFSSDEKQLFVADMSSVEFDEDGLHHLVVFDVDGNKL
                     KNRRDVAEISPGIPDGFRIKQNGVIFCSCEEGIIALLPDGTELGRFVLGKLTSNCSFG
                     DDEQTLFITCSNSVYRLTIK"
     gene            16135..17604
                     /locus_tag="PXB27_001087"
     CDS             16135..17604
                     /locus_tag="PXB27_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HDL5345889.1"
                     /translation="MDNKMNYTLGIDCGGTFIKAALFDTQGNIQALHRENVSVISEQA
                     GYAERDMSQLWAICAEVVRSTIEKSGVNPADIKAVGISAQGKGAFLLDKQNRPLGRAI
                     LSSDQRSLEIVKQWQAEGIPQQLYPITRQTLWTGHPVSILKWVQQNEPERYAQIGAVL
                     MSHDYLRFCLTGELHCEETNISESNLYNMEKGEYDQQLADLLGMPDVLEKLPPVIEPN
                     KISGYVTENAAKLTGLAVGTPIVGGLFDVVSTARCANLDDETKLNAVLGTWSVVSGIT
                     DHIDASQSLPFVYGRYAEAGKFIVHEASPTSAGNLEWFVNQWKLDYVKINQAVAQLPP
                     AASSVLFVPFLYGSNAGLGMQAGFYGMQAHHTQAHLLQAIYEGVLFSLMSHLNRMFIR
                     FPKADVLRVTGGPVKSKVWMQMLADLTGMRLEIPQMEETGCLGAALMAMQGVGLDISQ
                     VQALNNEMQVFEPNPENYAAYQTKYQRYQKLTEALNTMI"
BASE COUNT         4958 a         3868 c         4153 g         4702 t
ORIGIN      
        1 aaaaagaaga aagaaatagg caaaataact aatttttact tgaatgcaaa cgtttgcttg
       61 attaaactta gtcgcctact tctaaggcta cacagataaa atttgatgat taggagtaaa
      121 acaatgttca aaccagcaat tctggttctc actgacggtt ctgtcttttc cggcaaatcc
      181 attggtgctg acggtcacac tatcggtgaa gtggtattta acactgcgat gaccggttat
      241 caagaaatcc ttaccgatcc ttcttatact aaacaaatag tcaccctgac ttacccccac
      301 attggcaata ccggcattaa tttagaagat aaagaatccc ccaatgtttg tgcctccggc
      361 ttaattattc gtgatcttcc tcttctccat agcaacttcc gtgcgtcctc ctcactttca
      421 gattatctca ttcaacaaaa cattgtcgct attgccgata ttgatacccg ccgtttaacc
      481 cgaattttac gcgaaaaagg ggcacaagcc ggttgtattt tagtaggcga tgccacagaa
      541 gagaatattg ctaacgcttt ggcatttgcc aacagtttcg gttctatggc tgggaaagat
      601 ttggctcaaa gtgtcacctg caatgaatta tataaatgga cagatggtga atggcaatta
      661 ggtaaaggtt atgtaccgca acaacaaacc gagttccacg tagttgccta tgattacggt
      721 gtaaaacaca acattttgcg aatgcttgcc gaacgaggct gtcgcttaac cgttgtgccg
      781 gcaaaaacct ctgctgaagc tgtgcttgcg ttaaatccgg acggtatttt cctttctaac
      841 ggcccggggg acccggagcc gtgcgattat gccattaccg caatccgaaa attattggaa
      901 acgcaaaaac ccattttcgg catttgctta gggcatcaac ttttaggttt agcggcaggc
      961 ggtaaaacca aaaaaatggt gtttggtcat cacggagcta atcacccggt acaagattta
     1021 gacagccaaa aagtgctcat caccagccaa aaccacggtt ttgaagtaga tgaatccagc
     1081 ttaccatcta atgtgcgagt gactcaccgc tcactgtttg acggcacggt gcaggggatt
     1141 gaactcactg atagaccggc gttttcgttc caaggtcacc cggaagccag cccggggccg
     1201 aatgatgttg cctatttatt tgaccgattt gtcagtgaga tgaaaaaagt gaagtaagga
     1261 cgtacctatg tgtgtccatc aagcggtcga aatttgcaaa aaacttacca aaaatgaccg
     1321 cttgtagcct gaaataaacg agataaaaga gagcccaaaa tgccaaaaag aacagacata
     1381 aacactatcc taattatcgg tgccggtccg attgtgatcg gtcaagcgtg tgaatttgac
     1441 tactccggag cccaagcgtg caaagcgttg cgtgaagaag ggtataaagt ggtgttggtg
     1501 aactccaacc ctgcaaccat tatgaccgac ccgaatatgg cagatgtgac ttacattgag
     1561 ccgattgaat ggaaaacggt tgaaaaaatc attgaaaaag aacgccctga tgccatttta
     1621 ccgactatgg gcgggcagac agcgttgaac tgtgccttag atctttcgaa aaacggtgtg
     1681 ctgaaaaaat ataatgtaga gctgatcgga gctaccgaag acgcgattga taaagccgaa
     1741 gatcgtggtc gttttaaaga agcaatgact aaaatcggct tgaatacgcc aaaatcattc
     1801 gtttgccaca gttttgaaga ggccttagca gcacaagagc aggttggctt tccaaccctg
     1861 attcgcccct ctttcacgat gggaggctcc ggcggtggta ttgcttataa tcgagatgaa
     1921 tttatcgcca tctgcgaacg tggttttgaa gcctcgccta ctcacgaatt actgattgaa
     1981 caatccgtac taggctggaa agagtatgaa atggaagttg tgcgtgaccg tgccgataac
     2041 tgcattatcg tctgctcgat tgaaaacttc gacccgatgg gtgtacacac cggcgactcc
     2101 attaccgttg ctccggcaca aacgctaacc gataaagagt atcaaattat gcgtaacgct
     2161 tcgctggcgg tattgcgtga gatcggcgtg gacaccggcg gttcaaatgt gcaatttgcg
     2221 attaaccctg caaacggcga gatgatcgtc attgaaatga accctcgtgt gagccgttct
     2281 tctgcactcg catctaaagc caccggcttc ccgattgcca aagttgcggc gaaattggct
     2341 gtaggctaca cactcaacga attgcgtaac gacatcaccg gagggctgat tccggcttca
     2401 ttcgagccga ctttagatta tgttgtgacc aaaattccac gcttcgcctt tgaaaaattt
     2461 gccaatgcag acgaccgatt aaccactcaa atgaaatcgg taggcgaagt aatggcaatg
     2521 ggaagaacct tccaagagag tttacaaaaa gccttgcgag gcttggaaac cggcatttgc
     2581 ggctttaact tgctttccga agagcctgaa aaaatccgcc gtgaacttgc caaccccgga
     2641 ccaaaccgta ttttgtatgt tgccgatgcc tttggagcag gcttttcatt ggaagaagtt
     2701 caccactatt ctaaaatcga cccgtggttc ttggtgcaaa ttcaagactt agtacttgaa
     2761 gaattagcgt tagagaaaaa acagtttagc gatctagatg ctaacgaaat ccgccgctta
     2821 aaacgcaaag gcttctcaga caaacgtatc gcacagctta ccaaagtcag cgaaaaaatg
     2881 gtgcgggatt accgccatac ttacgctatt ctaccggtgt ataaacgggt agatacctgt
     2941 gcagccgagt ttgcctcaga tacggcttac ctctattcta cttacgagga agagtgtgaa
     3001 gccaaaccgt ctgatcgcaa aaaagtgatg attttaggcg gcggaccaaa ccgtatcggg
     3061 caggggattg aatttgacta ctgctgcgtt cacgcctcgc ttgctctgcg tgaggcaggt
     3121 tatgaaacca ttatggtgaa ctgcaaccct gaaaccgttt ccaccgattt cgacacctcc
     3181 gaccgcctct atttcgagcc gctaacatta gaagatgtac tggaaattgt gcgtgtagaa
     3241 aacccgtggg gcgtgattgt gcattacggc ggtcaaacac cactgaaatt agcgaatgcc
     3301 ttatctgatg ccggtgtaaa cattatcggg acttccgccg acagcattga tgccgccgaa
     3361 gaccgtgaac gtttccaaca aattctaaac actctcggtt taaaacagcc ggcaaatcgc
     3421 accgcccgta atgcgttgga agcggtggca ttggcaaatg aagtaggcta tccgctggtg
     3481 gttcgcccat cttatgtgct aggtggtcgg gcaatgcaaa ttgtgtataa cgatgaagag
     3541 ctaaaccgct atatgcgtga agcggtatcg gtttccaacg acagcccgat tttactcgac
     3601 cacttcctca ataatgcgat tgaagttgat gtggattgca tttgtgacgg cgaaaatgta
     3661 ctcattggcg gcattatgca acacgttgag caagccggta ttcacagtgg cgatagtgcc
     3721 tgctcactac ctgcctattc gttaagcggt catgttttgg acgaaattcg caaacaaacc
     3781 aacgcaatgg cgtttgctct cggtgtgaaa ggcttgatga acgtacaatt tgcagtacaa
     3841 aatgaggtcg tttatgtgtt ggaagttaac cctcgagcca gccgtactgt accgtttgtc
     3901 agcaaagcta ccggtcgccc actggcaaaa atcgctgctc gcgtgatggc tggtgaaacc
     3961 cttgccgact taaatgcatt agaggaaatt atcccaagca aattcttcgt taaagaagcg
     4021 gtattcccat tcatcaaatt cccgggtgtg gataccattc tcgggcctga aatgcgttct
     4081 accggcgaag tgatgggcat aggtaatacc tttgctgaag cgttcttcaa agcacaactg
     4141 ggtgccggtg aacgaattcc aaaagtcggc aaagtgttta tgtcggttat tgaagagcat
     4201 aaacctgctt taatttacat tgccaaacgt ttccaagaac aaggctacgg tttatgtgcc
     4261 accttcggca ctgcaaactt cctacggcaa cacggaattg cagtccaaac ggtaaacaaa
     4321 gttcgtgaag ggcgaccgca tattgtcgat gcgatcaaaa acggcgaaat tgcgttggta
     4381 atcaacaccg tcagcggcga gcctgaagtg attgccgaca gccactctat ccgccgtacc
     4441 gccttgcaac aacgtgtgcc tatttacacc acccttgcag gtgctgatgc tatctcctac
     4501 ggcgtaaaac atatcaatga gtttgatgtg tattcagtgc aggaattaga acaaaaggct
     4561 aactaaaata aagctaaaat gacaagcggt cagattttgc aaatttttta caaattctga
     4621 ccgcttgtgt ttgtagagga ttatttagta aatttatatg ctaaatcatt ccagcgtaat
     4681 tcatttttga gttgtggtag ctcggtattt tggttgatat ggataaactc aataccaaaa
     4741 tattcagcga acattcgtag catatcggca tcaatatcta aactaaatac actatgatga
     4801 gcaccgccgg ctagaatcca tgcttgagta cctacataaa aatttggttg aagcttccaa
     4861 aaagcatgcc caactggtaa atttggcatt ggatttggtg aggcgatagc gtctaaatcc
     4921 gcaacaacca ttctaaaacg gtctcccata tctacaacgg ttgcattcac tgcttttccc
     4981 gccttactac tgaaaattaa gcgaacagga tctgccttac cgccaatacc tagcggtcga
     5041 atggctaatg ttgatttctc tttggcaata gacggacaaa cttcaagcat atgagccccc
     5101 aataccactt ggttttgttc atctaaatca taggtgtagt cttccataaa ggaagaaccg
     5161 tttggtaagc cataacccat cactttcact tcacgcacaa gagctgcggt tttccaacct
     5221 ccttcaccac caaatccgta gccttgttcc attaaacgct gaacagccaa gcccggtaat
     5281 tgtttaatcc catttagatt ttggaaatta tcagtaaaag ctttaaatcc accattatct
     5341 aagaatgttt ttaagccgat ttcaatacga gcagaatatg cgagagattc tcttgctgaa
     5401 ccattttctt gcaagctatc ctcaacatta tacaagcggt cagattttgc aaaaaaatga
     5461 ccgcttgttg ccatttctaa agtgtgatct acctctcaaa tttacaaaat tttttataac
     5521 atattgattt aaaatgtttt ttatattttt cctttaattg actttaattt gtcttaagag
     5581 taacttataa aacgtgattt tactatttga aatattgagg tattcaagat gactattcga
     5641 gtttcctatg aagaattaaa agctcaattt aaacgtgttc tgttggctcg caatgtgcgt
     5701 gaagagattg ccgaagagtg tgccactgtg tttgccgaca ccactcaagc gggtgcttat
     5761 tcacacgggg taaaccgttt cccacgcttt attcagcagt tagagaacgg tgatattgtg
     5821 ccggaagccg tgccgactaa agtgctttca ctgggggcga ttgagcagtg ggacgctcac
     5881 caagccatcg gtaacttaac cgcaaaaaaa atgatggaca gagcaatgga gcttgcatct
     5941 gaaaacggca tcggcgtagt ggcgttacgt aatgcaaacc actggatgcg tggcggttct
     6001 tacggatggc aggcggcaga gaaaggctat atcgggatct gctggacgaa cgcccttgcg
     6061 gtaatgccgc cttggggggc gaaagagtgc cgtatcggga ctaacccgtt aatcattgcg
     6121 gtgccgacta acccaatcac gatggtggat atgtcttgct caatgtactc atacggaatg
     6181 ttggaagtgc atcgccttgc tggtcgtcaa acctttgtcg atgctggttt tgatgatgac
     6241 ggcaacccaa cccgtgatcc ggcaacggtc gagaaaaacc tccgtctaat gccgatgggc
     6301 ttctggaaag gttcgggctt atcgattgtg ttagatatga tcgccacctt gctctctaac
     6361 ggtgaatcca ctgcggcagt caccgaagat aaagacgatg aatattgcgt gtcgcaagtt
     6421 tatatcgcta ttgaagtgga tcgcttaatt gacggcaaaa ccaaagacga aaaactcaac
     6481 cgtattatgg aatatgtgaa aaccgcagaa cgtgccgacc ctgatgtggc agtacgttta
     6541 ccgggccacg agttcaccca aattctagcc gataacaaag ccaacggcat tccggtggat
     6601 gacaccgttt gggcgaaatt acaggcgttg taattgcaaa ttgaccggta gggtgggcgt
     6661 aagcccacgc attagacaca atcataagat gacgtgggct tacgcccacc ctacaagcgg
     6721 tcatttttcc gcagaatttt acaaaacgga ggtatgaatg ttttttggtc atatttcaca
     6781 agttaaagcg aatcgatacc cgccggcgat ccaaattgcg ttgaactatc tgaaaaatac
     6841 cgattttgat gcgatggaag caggggttta cgaactcaaa ggcagacaaa tttatgtgca
     6901 ggtgttggat ttgaacacca aatcgaaaca cgaatttcag ccggaagtgc atcgcaatta
     6961 cctcgatgtg cagtatctac accgaggcaa agagataatg gctgcggcgg tggataccgg
     7021 caccaatccg attgcgatgg aatacaaccc tgagcgggat attcaatatt atcaatccgt
     7081 tgccaacgaa aatgaatttc gctgcgttga aggcaatttt tgacggcacg gaaaccattc
     7141 gcaaagtggt ggtaaaaatt gcgatgagcg aaattgagga gcaacgatga aagccttagc
     7201 tcaattagtc ggaaaactgt tagaaatctt ggtggtggtg attttatccg caatgtcgat
     7261 tttggttttc gtcaatgtgg tactacgtta cggttttaac agcagcatta gcgtgaccga
     7321 agaggtgtct cgctatatgt ttgtttggtt ggcgttttta ggggcggtgt tggcatttag
     7381 cgaaaatcaa cacgttaaag tcacaatgct gaccgataaa ttatcgccaa cggctcgtaa
     7441 tatcttaagc gtagtcacag atttagcgat gttgttctgt tgctacttaa ttgtggacgg
     7501 tagctggatt caatttaacc tcaacctgaa taactttgcc ccgatttcag gcttaccgca
     7561 aggcatcact ttccttgcca gcacagtggc aggggtatta atcggtttat taattattgc
     7621 ccgcattgtg gcaacggttg gcgtgattgc aaaaggagaa aaatagatga ctgtggtaat
     7681 ttttctttcc gtcttgcttg gctcgatttt attgggcatt ccggtagctt tcgccctgtt
     7741 ggtctgcggt gtggcgttaa tgttgcattt agatctgttc gatgcccaaa ttttggctca
     7801 acaaattgtg agcggtgcag acagtttctc gctaatggca attccgttct tcattcttgc
     7861 cggtgaaatt atgaacgagg gcggtctctc caaacggatt atcgacctgc cgatgaaatt
     7921 agtcggtcat aaacggggcg gtttaggttt tgtagcaatt ttagcggcga tgattatggc
     7981 aagcctttcc ggttcggcgg tggcggatac cgcagcggta gtcgcaatgc tactgccgat
     8041 gatgaaaacc accggttatc cgattgaccg ctctgccggt ttaatcggta cggcagggat
     8101 tatcgcccca atcattccgc caagcattcc gtttatcgta tttggtgtgg ctagtggcgt
     8161 gtccattacc aaactgttct tagccggtat tttcccgggc attatgatgg ggatctgctt
     8221 ggctgccctt tggtggtggc aggcaaaacg tttggattta atgaccttct ccaaagcgac
     8281 caaagaagaa ctttgcattt catttaaaaa cagtatttgg gcgttattgt tgcctgtcat
     8341 tattatcggt ggtttccgct cgggaatgtt caccccaacc gaagcgggtg cggtcgcaac
     8401 tttctatgcc ttgattgttt cactgtttgt ttaccgtgaa atgaagttca aagatttata
     8461 caaggtagtg ttggcggcag cgaaaaccac ggcagtagtg atgttcttag tggcagcggc
     8521 aaatgtgacc ggctggctga ttaccgtagc ggaattaccg atgatgctca ccgagctgtt
     8581 agagccgtta attgaaagcc cgaccacact cttattggtg attatgttag cggtgtttat
     8641 tatcggaatg gtgatggact taaccccaac cgtgctgatt ttaacgccgg tattaatgcc
     8701 gttggtggaa gaggcaggca ttgacccggt ttacttcggt gtgctgttta ttcttaacac
     8761 ctccatcggc ttgattacac caccggtcgg caacgtgcta aacgtgatta ccggcgtgtc
     8821 gaaactgccg ttcgaccaag cggctaaggg cgtaatgcct tacctcgtaa tgatgatttg
     8881 tttactactg ttgttcgtgt tcttcccgtc actggtgtta gtgccgttaa gctggatgca
     8941 gtaatggttt gaaccgaaac aagcggtcga attgacgcaa aaatttgcaa atttttttgg
     9001 caaaacagac cgcttgcaac tcaattttta atcacaggag aaccctatga aattatttaa
     9061 tttaaaaacc ttatttgcct tagtggcagg cgttgcggta atgagcggct ctgcaatggc
     9121 agaaacctca ctgcgttttg gttatgaagc cccacgttcc gatacccaac acatcgccgc
     9181 caaaaaattc gatgaattat tggcagcaaa aaccaaaggt gaaattaaat taaaactgtt
     9241 tccggacagc accttaggta atgctcaaac gatgatcagc ggcgtgcgtg gcggtactat
     9301 cgacattgaa atgtccggct cgccaaactt taccggctta gagccaaaac taaatgtgat
     9361 tgatattccg tttattttca aagatcgtga acacgtttac aaagtattag acggcgaagt
     9421 tggtcaaggc ttattaaaag atttagaagc ccaaggctta aaaggcttag ccttctggga
     9481 cgttggcttc cgtgctttct ccaactcaaa acaccctgtg accaagccgg aagacatcaa
     9541 aggcttaaaa gtccgcacca accaaaaccc gatgtacatt caagccttca ccttactagg
     9601 cggcaacccg gtgccaatgc cactttctga gctttacacc gcactcgaaa cccgtgccgt
     9661 tgatgcccaa gaacacccaa tcggcatttt ctggtcggca aaactgtttg aagtacaaaa
     9721 acatttaagt ttaaccaacc acggctacac cccgttaatc gtggtgatga acaaagcgaa
     9781 gtttgacggc ttagctcctg aattacaaac cgcattagtt gaagccgcaa aagaagccgg
     9841 tcaattccaa cgtgatctca acgtgaaaaa cgaaaaagag atcatcgaaa aattacgcaa
     9901 acaaggcatc gaagtgatcg aacaagtgga taacgccccg ttcaaagcag cgattgaagc
     9961 ggaagtccgc aaagctttcg tggaaaaaca cggcgatgaa ttattgaaaa aagtggatgc
    10021 gttagcgcag taattgactt tattttagtc taaagctccc tccgtttggg gggagttgtt
    10081 gtatagactc tactatagga gagactatga aactgtttaa tttaaaaact ttagccgctt
    10141 tcgttgccgg ttttgcactg ttttcaaccg cacaagcaga aacctcattc cgctttgcgt
    10201 atgaagctcc tcgttctgat actcaacata ttgcagcgaa aaaatttaat gatttgctgc
    10261 aagaaaaatc ccaaaaaggg ctgaaattaa gcctcttccc tgatagcacg ctcggcaacg
    10321 cacaaacggc gattagcggt gtgcgtggcg gtactattga tattgcaatg tccagctcct
    10381 ctaactttac cgggttagaa ccaaaactga atgtgattga tattccgttt attttcaaag
    10441 acagagacca cgcctataaa gccttagatg gagagattgg tcaaggttta ttaaccgcat
    10501 tagaggctca cggtttgaaa gggttggcgt tttgggaagt tggtttccga gcgttttcca
    10561 actccaagca cccgatcacc aaaccggagg atattaaagg cttaaaaatc cgtactaacc
    10621 aaaacccgat gtatattcaa gccttctcta ttttaggtgc aaatccggtg ccaatgccgc
    10681 tttccaagct ttacactgca ctggaaacca aagcggttga tgcccaagaa cacccaatcg
    10741 gcattttctg gtcggcaaaa ctctatgaag tacaaaaata tttcagcttt actaaccacg
    10801 gttacacccc gttaattgtg gcgattaaca aagcgaaatt tgatgcactg ccagccgagc
    10861 aacaaaccgc attagttgaa gcggcaaaag aagccggaaa ataccaacgc caacttaacc
    10921 tagaaaatga gcagaaaatt atcgactcgc tcaaaaaagc aggtattgaa tttgtggata
    10981 acttagatac cacaccgttc aaagatgccg tatcggctga aacccgcaag gcatttatcg
    11041 agaaaaacgg tgatggctta gttaaagcca ttgatgcatt ggcgaagtaa aaatagagct
    11101 acaaagtgct cggttaaact aacctcacac gaatcgtgtg aggtacaagc ggtcaaaatc
    11161 cgcaaaattt ttacacatct ttcctcaaag gaggaacaaa tggcacagcc cattttaagt
    11221 atgcagcaca tcatcaaacg ctttggtagc catacggcgg tgaatgatgt cagccttgat
    11281 atccactcag gcgagatcgt tgccttactc ggtgaaaacg gtgcaggtaa atcgacccta
    11341 atcaaaatcc ttgccggtat ttatgaccgt gacgagggtg atattctttt ccataaccaa
    11401 aaaatccaat ctgcccacgc tattgagaaa ggcaacaaaa agcccatcgc ttttatccac
    11461 caagatttag gcctaataga atggatgacg attgccgaaa atatggcatt tgtaatgggg
    11521 ttccctcgca aatttggctt gattgactgg gcgaaagtgc gagagcagtc gcaaaaagcc
    11581 cttgattttg taggcattaa actctctgcc gacactcgag tgtttgattt aagccgtacc
    11641 gaaaaagcct tactcgccat tgcccgtgcg attgccgtca atgcagaaat cttggtttta
    11701 gacgaaccta ctgcttccct acctgcctct gatgtagagc atcttttcac tgtgttaaac
    11761 cgcttacgtt cccaaggtgt gggaatgatt tatgtgaccc accgtttaga tgaagtgatc
    11821 gccatttccg accgcatttt ggtgatgcgg gacggtttcc cggttgcaga agggcaaacc
    11881 aagctctatg acgtcaaagg cttggtcaaa gcgattgtag gcgaagagag ccgtggcaga
    11941 cagcgggtgg atttaccaac cgatactcct gaaattttaa gcctgcaaaa tgtcgtggtt
    12001 ggcgacaccg gcccggtgaa tttccgtctg cacaaaggtg agatgattgc ccttgccggc
    12061 ttgcgtggag cagggcagga agaaatcggc cgcttattat ttggggagcg aaccttagac
    12121 aaaggcgaaa ttcagttaga caagcggtca tattctgcta aagatccgca agatgcgata
    12181 gccaaaggtg tggcattggt tgcgggagac cgcacccgtg aaagcgtgtt tatgtcaatg
    12241 actaccagtg agaacctgtt tatcaacccg gtactaagcg gtcattcacc attaaaacgc
    12301 tactcccgcc gtgaagagtg gggtgagagc tggtacaaat tccaactatt cgatattcgt
    12361 ccgaaaaatt tgtttattga tgccagtgcg ttatccggtg gtaatcagca gaaaatcgtg
    12421 ttggcacgtt ggatgcacct caatacgcct gttctgattt tagaagaccc gactgccggc
    12481 gtggatgtag gagcaagggc agagatttac gacttgctca acaaagccct tgcagaaggg
    12541 gtcgcaatta ttgtgatttc caccgacttt gaagagatcg ctcatttgtg taaccgagca
    12601 ttagtcttca accgagggga aattgccggt gaactactga acgaagaggt gactttcgcc
    12661 aatttgctgg cgttggcatc ggatagtcag tcgaaaaaac aataacttac tctcgcccgc
    12721 ttgcgggaga gagacagcta aaaatagcgt caggctattt ttagcagaga gagggtaaac
    12781 aagcggtcat ttttcttaaa aaatattgcg aaccctctcc ctccgaaagc ctaacggctt
    12841 tctcctccct ctcccgcaag cgggcgaggg gaaaatgcag aattttaacg gagtaattta
    12901 tggctcaaac aaacatcaaa tcaaccgcac tggaacaacc ggtatctatc gcccgtgacg
    12961 gttttatggc atggttttca caaatgtcca ctcgttatgg cttactgtgg ttatgtgtgt
    13021 tattggtgat tattttctca cttaccaccg aatctttcgc ctcaatgctg acattaaatg
    13081 cgattttaga aagtaaatcg aaaattgccc tacttgcttt ggcagccaca actacaatga
    13141 ttgtcggcaa aatcgacctg aacgtgggtt tcggtattgt gttgtggcac attcttgtga
    13201 ttactctgca agtccaatac ggcttctcgt ggtatatggc gatcttaatc gtgttagtga
    13261 ttgccgctct ttacggcttg ctgaatggga ttttagttgc tcttgctgat atcgatagct
    13321 ttgtggcaac acttggctca ggcacagtgc tttatgccgt agcactctgg cactcgggcg
    13381 gtcgccaaat tgtcggggat ttacccgatg aatttatcgc actcaacagc acagaaattt
    13441 tcggtatccc gatttctgct ttctatgtgc tggcagtggc tatcgtaatg tggttggtga
    13501 cagaacatac cccgaccggt cgttgtatgt atgcggttgg tggcaaccca acggcagctc
    13561 gcctaaatgg tatcaatatt aaaaaataca ccattgtgcc gtttattgtc tctagtgtaa
    13621 tcactgcttt cacaggcgta ttgatctctg ctcaacaagg cgttggtcag gcaagtgtcg
    13681 gtatggacta cttactgccc gcattggttg gggcattctt agggagtacc actattcgcc
    13741 cagggcgtat taacgtatgg ggaacagtgg tggggattgc cattcttgcc atcggtattt
    13801 caggtattca acaattcggt ggagcgttct gggttgagcc actctttaac ggtgcaacac
    13861 tgttactttc catcactatt gcaggctatg cccaacgcaa acgcttgtta aatcaaaaag
    13921 ctgtacagaa aaaatcgacc aactaagagg atttgattat gaaaaaagca tttcgtacaa
    13981 ccttaactgc aatcgcaacc ttaactgcta ttgctttcgg aaataatgcg tttgccaatg
    14041 acgacccgtt cgtacaagaa gcaaaacagc ttgtcgcagc agctacggct aagcaagaaa
    14101 aatgggacgg accgacaacc ggccctcaat tacaaaaaga gaaaactgtg atttttattg
    14161 cctctgatat gaaaaacggt ggtgtacttg gtgtgattga cggaatgaaa gaagctgttg
    14221 gcgtaacggg ctggaagtta gatgtattag acggtgcagg tacggtaaac aaccaactcg
    14281 ccgcacttaa ccaagcgatt gcccgcaaac cggatgcgat tgtgattggt ggctggaacc
    14341 cgaatgtggc gaaaatccca ctacaaaaag cgactaaaaa cggcattaaa cttgcagctt
    14401 ggcacgcaac accggagccg ggtgcgattg ataaatacgg cattttctac aacgtgacct
    14461 ctgactcaga cgaaattgca cgcttatcgg caatgcttgc ggtggcagaa tctaacggta
    14521 aggcaaaagc gattatttta accgactcgc tatatgaaat tgcgttacgc aaagcgaacg
    14581 tcatgaaaga agttatcgaa aaatgtaaag agtgtaaggt gttagagttt atcgacacgc
    14641 cattggcgga tacctcaagc cgtatgccta acctcacttt cagcttactg caaaaatatg
    14701 gcgacgattt agagtatgcg ttggcaatta acgatctcta ctttgacttt atggctccgt
    14761 ctttacgcac cgctggtaaa gataaaccgt ttagcatttc cgcaggtgat ggctcagtaa
    14821 cggcttatca acgtattcgc aatggtgata aacagtttgc aaccgtccct gagccactta
    14881 acttacacgg ctggcagtta gtcgatgagc taaaccgtgc tttcgctaac gaaaaaccgt
    14941 ctggttatat cacaccggct cacctagtga ctaaagaaaa tgtggcgttt gacggtggtg
    15001 aaaacaacct gtatgcccca caaaacggtt acaaaggcgc ttacaaagcg atttggggcg
    15061 tgaagtaatg acgtaggggc aaataaatat ttgcctcgaa caagcggttg ttttttgcaa
    15121 agattttgcg aaatttaacc gcttgtcata aaaaatagag agggtaatac aaatgagcaa
    15181 taaatttatc cccaaaatct ccaaccctcg ttttaatgag ctggttggga atacgtttga
    15241 acttgaagag ctttttgata aggcaatctg ggcagaaggg ccggcgtgga gcaagccaca
    15301 acaagccctc tattttagtg atgtaaaagg ttgcacaatg taccgctgga cagagcaaga
    15361 tggtacacag gttttccgcc agccgtctta tttttctaac ggcaatgcgt ttgatgcaaa
    15421 cgacaacttg attacctgtg aacacggcaa tcgaggtatc agtttaacca cgccaagcgg
    15481 tgaatatcgt tttttggtcg gtcaattaga cggaaaacga ctgaactcac ccaatgatgt
    15541 ggtggtaaaa tcagacggca tcatttggtt cactgatcca ccttacggta ttttgtcgga
    15601 taacgaaggc aaacagtcgc caagcgaaat tatcggttgt tatatttact gctatgatcc
    15661 gaaaaccgac aagctgaata ttgcaacttt caacacgatg cgaccaaacg gcttggcctt
    15721 ctcgtcagac gaaaaacaac tctttgtggc agatatgtcg agcgtggaat ttgacgaaga
    15781 tggcttacac cacttagtcg tatttgatgt ggacggtaat aaactaaaaa atcgccgaga
    15841 tgtggcggaa attagccccg gcattccgga cggcttccga attaaacaaa acggcgtaat
    15901 tttctgctct tgtgaagagg gaattattgc tcttttacct gacggcacgg agctgggacg
    15961 atttgtgctg ggtaagctaa cctcaaattg tagctttggc gatgatgaac aaacactgtt
    16021 tattacttgt tcgaatagcg tttatcgttt aacaattaaa tagcagaatg cattaagtgc
    16081 gtctgttgta gcattacaag cggtttcttt ttcaaaattt tttacaaaca aataatggat
    16141 aacaaaatga actacacact cggcattgat tgcggtggga cttttatcaa agccgctctt
    16201 tttgatacac aaggtaatat tcaagccctt catcgtgaaa acgtttcggt aatcagtgaa
    16261 caggcaggtt atgcagagcg tgatatgtca caactctggg ctatttgtgc ggaagtagtg
    16321 cgttcaacca ttgaaaaaag tggggtaaac ccagcggata ttaaagcagt agggatttcc
    16381 gctcaaggca aaggggcgtt tttactggat aagcagaatc gaccgttggg gcgtgcgatt
    16441 ttatcttccg accaacgttc ccttgagatt gttaaacaat ggcaggctga aggcattccg
    16501 caacaacttt acccgattac ccgccaaact ctttggacag gacaccctgt gtcgattttg
    16561 aagtgggtgc agcaaaatga accggagcgt tatgcacaaa tcggggcggt gttgatgtcg
    16621 cacgactatt tgcgtttttg cttaaccggt gagttgcatt gcgaagagac caacatttcc
    16681 gaaagcaatc tttacaatat ggaaaaaggc gaatacgacc aacagttagc cgatttgttg
    16741 ggaatgccgg atgtgttgga gaaattacca ccagtgattg aaccgaataa aatttcggga
    16801 tatgtgactg aaaatgccgc caaattaacc ggtttagcgg tgggtacgcc gatagtcggt
    16861 ggcttgtttg atgtggtttc taccgcacgt tgtgccaatt tagatgacga aaccaaactg
    16921 aatgcagtgc tgggaacgtg gtcggtggtg agcggcatta ccgatcatat tgatgcaagc
    16981 caatcattac cttttgttta tgggcgttat gccgaggcag ggaaatttat cgtccacgaa
    17041 gcaagcccga cttctgccgg taatttagag tggtttgtga atcagtggaa actcgattat
    17101 gtgaaaatca accaagctgt tgcccaatta ccgccggcgg caagctcggt gctgtttgtg
    17161 ccgtttctgt atggctcaaa tgcaggctta ggaatgcaag ccggtttcta tggtatgcaa
    17221 gcccaccata cccaagccca tctcttgcag gcaatttacg aaggagtgtt gtttagcttg
    17281 atgagccatt taaaccgtat gtttatccgc tttccaaagg ctgacgtgct gcgagttacc
    17341 ggcggaccgg tgaaatctaa agtttggatg caaatgttgg ccgatttaac cggaatgcgg
    17401 cttgaaatac cacaaatgga agaaaccggc tgcttgggtg cggcattaat ggcaatgcaa
    17461 ggggttgggc tggatataag ccaagttcaa gccctaaata atgaaatgca ggtgtttgag
    17521 ccaaatccgg aaaattatgc ggcttatcag acgaaatatc agcgttacca aaaattaact
    17581 gaagcattaa atacaatgat ttaacctgaa tttatgcttt tacaagcggt aatatttggt
    17641 tacttttttg caaaaattac tgcttttttg tttatttaat t
//
LOCUS       DANLUQ010000042        17283 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.042, whole genome shotgun sequence.
ACCESSION   DANLUQ010000042 DANLUQ010000000
VERSION     DANLUQ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 17283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17283
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.042"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(49..651)
                     /locus_tag="PXB27_001088"
     CDS             complement(49..651)
                     /locus_tag="PXB27_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDL5345890.1"
                     /translation="METLIEFFSSYGYWAVFLVLLACGFGVPIPEDITLVSGGVISGL
                     GYTNVHWMLVVSMLGVLVGDSTMYWLGRIYGEKILKFPLIRNVATPERFATVQERFEK
                     QGWKLLFMARFLPGLRAVVYLVSGITRKVSFVKFLLVDFCAAIISVPIWVYLGDYGAS
                     NLDWLKEQVANGQHLIFAILGIVILFLGWKWYKNKKQYKK"
     gene            complement(653..1672)
                     /gene="purR"
                     /locus_tag="PXB27_001089"
     CDS             complement(653..1672)
                     /gene="purR"
                     /locus_tag="PXB27_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HDL5345891.1"
                     /translation="MATIKDVAKYAGVSTTTVSHVINKTRFVAEDTTKAVWEAIKALN
                     YSPSAVARSLKINTTKSIGMIITTSESPFFAEIVLAVEEFCYSKGYSLFLCNTQNKLE
                     KIQNHLDMLIKKRVDGILVMCSEYTANSFDIFENSNIPMVIMDWGRSDERSDIILDHG
                     VEGGYIATQHLIENGHQDIAVITGNRDKEIARTRFEGFKKALSEAGLSIREEWIQEGD
                     FEPEGGYECMNNLLKSSEKLPTAVFCFNDSMALGAISAITEKGLNVPQDISVIGYDNV
                     HSSRFYAPPLTTIHQSKSRLGSAALELLLERIEDNENAKEPKVLEFFPELVKRKSVRN
                     LNIKE"
     gene            complement(1808..2536)
                     /gene="cmoA"
                     /locus_tag="PXB27_001090"
     CDS             complement(1808..2536)
                     /gene="cmoA"
                     /locus_tag="PXB27_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HDL5345892.1"
                     /translation="MQRDTLFSAPIEKLGDFTFDESVAEVFPDMIQRSVPGYSNIITA
                     IGMLAERFVTDNSNVYDLGCSRGAGILSIRRNVNAKNVQIIGVDNSQPMVERCKTHLS
                     AYHSEIPVTILLDDIRHIEIKNASMVVLNFTLQFLPREDRLALLQKIYQGLNSNGILV
                     LSEKFTFESEEMNELLIDLHHTFKRANGYSELEVSQKRNALENVMRTDSIETHKKRLQ
                     EAGFTQVELWFQCFNFGSMIAIKA"
     gene            complement(2546..3328)
                     /locus_tag="PXB27_001091"
     CDS             complement(2546..3328)
                     /locus_tag="PXB27_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL5345893.1"
                     /translation="MTEKSQLNFNYQPSNNHANAQTMIFLHGLFGDLNNLGAIAKPFA
                     ERYSILMVDLRNHGRSFHSDEMNYALIAQDLAQLLTELNIQNAIVVGHSMGGKSAMAL
                     AAFAPELVEKLVVIDIAPVTYTQNRHQQVMAALFAVKEAKPQNRQQAKAIMAEYIRDE
                     GVQQFMLKSFDAQSEQSFRFNLQALKDNYQNVMGWQQVFVDKPTLFIKGALSDYIQAE
                     HTQTILAQFPQAKSFIVANADHWVHAEKTGTVVRAINKFLEK"
     gene            3447..4013
                     /gene="seqA"
                     /locus_tag="PXB27_001092"
     CDS             3447..4013
                     /gene="seqA"
                     /locus_tag="PXB27_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HDL5345894.1"
                     /translation="MKRIEIDDELYQYIASRTQSIGETASDILRRLLRLPQSPQPFVL
                     VQEHMINELKELVKTPSRATKKNESNAEKTVEKLENILNSEHFMNENKNVVRFTMLLA
                     ALYRANPEAFAKATEKVRGNERIYFSQSEAEILATGSGVKAKQIPDSPFWVITNNNTA
                     RKGLILKGVMESMKVPSQLIERIQALFI"
     gene            4023..5441
                     /gene="menE"
                     /locus_tag="PXB27_001093"
     CDS             4023..5441
                     /gene="menE"
                     /locus_tag="PXB27_001093"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HDL5345895.1"
                     /translation="MLINSTRFPTQFWAAHSAQNVAIIWEKGTSDYLNHLPSNITWYS
                     LNQLIAQAIFVLQQQGATPHSLIAYCGSHRLIGLLTYCASLALGAKILMLNPAQTASQ
                     RQAVLDDNGVELIINDADFAKFSAKTTACNAFPTIDFEKPATLTLTSGSSGKPKAVVH
                     SIAAHLYSAEGVCELMAFEQPNSWLLSLPLFHVSGQGIIWRWLFKGATLYVHEDKSDF
                     FLSAQKVSHLSLVPTQLQRYLNQLDSMVSQKCLLGGSMLPAELIAQAQQSGMTTFSGY
                     GMTEMASTICAVENDLNSVGFPLKHRELKLENGEIWVKGKSLGLGYWQKNGQIRPLVN
                     TQGWFATKDRGEWNHKKQLVIKGRLDNMFISGGENIQPEEIEQLIFRSQLVEQIIVLP
                     IEDKQFGHRPVAFLQFKQSDSKNELHKLKIWLSDKLERFKQPVAYYLLDIEKYQKQGL
                     IKLSRTQLQQDLKLKVLKEIYV"
     gene            5434..8811
                     /gene="mscK"
                     /locus_tag="PXB27_001094"
     CDS             5434..8811
                     /gene="mscK"
                     /locus_tag="PXB27_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HDL5345896.1"
                     /translation="MFRPILKTFVLSLGLMLNSYALAELPTQQAVQTQLDSAKKLDQA
                     DADNKALVQTLEDTLAFLAQIDKQKADNQALNKSIQDSQKALKTSQHNLEKLKEVTIA
                     SVDNLTQKATTDLQKELTSTQSQLETVQHDLSIINNNLVAQNTAPDKAQTALTEMVKR
                     KQEISSQLSATNIKNELQTKLEVELELIELKNTYNQILLGGSEDLSALYSAQLDEKKQ
                     EQQNLQAEIANLQTAINNKIVEESQNKLDQAQAVQNQQNNATTNPVILRELDINTKVT
                     QELLKQTKDMTQLSQDNLRIKSVLDNLQQTQRNIEEQISSLQGTLVLSRIINKQKQSL
                     PQDEMISGLSKQIADLRVRVFDITEFKDSFADINAYISRIEQDEKTTFTSKEKEQLSK
                     ILQERSDTLTEMIKSLNNQLNLLINIELNQQQVQTISDALQQKLQQQSFWVKSNNPID
                     LEWFSKFVDITHYQFIDIGKKLNFSNWRDNLLPASILVGILLLLAIFINRQKDKIKQK
                     LHQINNRLKIVATDSQWNTPLAIFWTLVLCLPSTLMFLAGFVAITYISFENPQEVWTW
                     GLKMAIYWLYFAFLVAMLRPHGIGHIHFNMPQKSNEIFRNILKRSVWIIALLLNTSIF
                     TNLEMGISYDMLGQTMTIFVLILMIWMIVPAFRQAISTYENVTTEEKKESKSILLTIV
                     KLVLLLAPIILIILIALGYYYTALVIIEHLVDSYFAIITWIIIRNILYRAFSISSRRL
                     AYRRLQEKREAAEAKTRAKAEQNDENPDGVELPFELKEDSIAVSEVKNQMLKLTDFVL
                     WIGLFVLLYWVWSDLITVALYLDGVTLWQQSVTTEAGIVMESITLLNLLTAILIIVVM
                     VVLIRNLSGLLEALIFSHFKLSQGTPYTITTLLTYLIIALGAIFAFGTLGMSWSKLQW
                     LLTALSVGLGFGMQEIFANFVSGIIILFERPMRIGDTITIGTYSGTVSKIRIRATTLV
                     DFDGKEVIVPNKAFVTERLVNWALSSTATRIIVQVGVAYGSDLELVKRLLLQVAEENP
                     KVLKDPQPRAYFLTFGASTLDHELRAYVNDLSDRNPTIDALNTRIHQLFNEHNIDIAF
                     NQLDVYIKNNDTQDEIKVDSKRFEFERPNNITAKEKE"
     gene            8814..9896
                     /gene="aroC"
                     /locus_tag="PXB27_001095"
     CDS             8814..9896
                     /gene="aroC"
                     /locus_tag="PXB27_001095"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012072711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDL5345897.1"
                     /translation="MAGNSIGQLFKVTTFGESHGIALGCIVDGVPPNMALSEADLQPD
                     LDRRKPGTSRYTTPRREDDEVQILSGVFEGKTTGTSIGLIIKNGDQRSQDYGDIAEKF
                     RPGHADYTYQQKYGIRDYRGGGRSSARETAMRVAAGAIAKKYLREQFGIEVRGYLSQI
                     GNIKIDPKTVENIEQIDWHAVNSNPFFCPDPVAIEGFDALIRELKKEGDSVGAKLTVI
                     AERVPVGLGEPVFDRLDADLAHALMSINAVKAVEIGDGFAVVEQRGSQHRDEMTPQGF
                     LSNHAGGILGGISSGQPIIAHIALKPTSSITIPGRSVNKNNEAVEVITKGRHDPCVGI
                     RAVPIAEAMMAIVLLDHLLRFKAQCR"
     gene            9915..10742
                     /gene="mepA"
                     /locus_tag="PXB27_001096"
     CDS             9915..10742
                     /gene="mepA"
                     /locus_tag="PXB27_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HDL5345898.1"
                     /translation="MKLLKTLLVSSLAFTATALNATQWEKIKTPVQGKAQSIGGYSNG
                     CIIGAQPLALKGEGYQVIRSARNRYYGHPQLLDYLTHLGKKTKAAGVPTILIGDMGMP
                     AGGRFASGHASHQTGLDADIWFKFGPMDDETARNPAGLATIMVNRSTERVDDSLWNQH
                     HTNLVKLAATDPRVDRIFVNPAIKVKLCQTAGNDRAWLRKIRPWYGHDSHFHVRLTCP
                     ADAASCENQALVPAGDGCGEELYSWFEPKEASGTPKAKVLPTAPVQCQMILSEQGLN"
     gene            10815..12734
                     /locus_tag="PXB27_001097"
     CDS             10815..12734
                     /locus_tag="PXB27_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL5345899.1"
                     /translation="MRLFVAEKPSLARAIADVLPKPHTKGDGFIQCGEHDTVTWCIGH
                     LLEQAEPDVYDERFKMWRMEHLPIVPEQWKLIPKKETLKQFKVVEKLIKKADVLVNAG
                     DPDREGQLLVDEVFGYLNLSAERKSQIQRCLVSDLNPNAVSRAIEKLQKNSDFIPLAT
                     SALARARADWLYGINMTRAYTLQGRWAGYKGVLSVGRVQTPVLGLIVRRDLEIEHFVP
                     KDYFEVLAQIVVPETQERFKAQWQPSKACEDYQDEDGRVLSRPLAENVVKRITDQPAT
                     VTEYQNKIEKETAPLPYSLSVLQMEAAGRYGLSAQAVLDICQKLYETHKLITYPRSDN
                     RYLPTEHFNDRFKVMAAIAHHLPEYQQKPEVIDATIRNRCWNDKEVEAHHAIIPTARQ
                     GTVHLTENEKRVYQLIARQYLMQFCPDAEYRKSKIGLEIAGGSFVAQARNLVIAGWRA
                     LLGKEDSLDDAEPLLPIVKKGQILHCEKGEIVSKKTQPPRHFTDRSLLSAMTGIARFV
                     QDKELKKILRETDGLGTEATRAGIIELLFKRGFLIRKGKNIHSTEAGRILIQALPDSA
                     TQPDMTAHWEMQLNQISKKETSYQQFMFDLNQQLPHLLQSPNRQVLQNLAKITPLASG
                     NKPRYFKKRTQTAEN"
     gene            12840..13166
                     /gene="secG"
                     /locus_tag="PXB27_001098"
     CDS             12840..13166
                     /gene="secG"
                     /locus_tag="PXB27_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDL5345900.1"
                     /translation="MLINVLTIVYLVVAVLLIAFVLMQQGKGADAGASFGAGASATVF
                     GSAGSANFLSRTTALLATIFFAISLVIGNLSSHNVAPKSQFEDLSQVEQKQVEPVKIE
                     NSDIPQ"
     gene            13191..13276
                     /locus_tag="PXB27_001099"
     tRNA            13191..13276
                     /locus_tag="PXB27_001099"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13225..13227,aa:Leu,seq:gag)
     gene            13479..14720
                     /locus_tag="PXB27_001100"
     CDS             13479..14720
                     /locus_tag="PXB27_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL5345901.1"
                     /translation="MARTTKALNKTQIENAKSKDKEYILSDGDGLNLRVKPLPKGSKI
                     WIFNYTNPQTKNRTNLTIGRYPLITLAEARTICDGYRSLIEKGIDPQEEKKRLQQEAE
                     NRVNDTFRNVAESYFNGIYKEKAKNPITREKNWIRLENHIFPYIGDKHVEEIKVKALV
                     DIYSKVADKSNTLKKLHQLVSAVMDHAITQGIIESHNCRLAVKNFYIKPSTPNPTIEL
                     EELPQLFTDLHNAKLSKQTYALVCWSFLTALRPNEAVNAEWKEIDFEKKLWNIPKEKM
                     KGKADKKRPHSVPLSSQALKILEMMKMHSNSSPFVFPGRSSNSKPMSNATVNSTLKRN
                     GYKDRLTAHGIRAFVKTFLSSRKVDRHVSESILSHLLEGGDDLENTYNRYDFLEERVP
                     VMQLLGDYCEQCGMNLELVRG"
     gene            complement(14879..16747)
                     /locus_tag="PXB27_001101"
     CDS             complement(14879..16747)
                     /locus_tag="PXB27_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL5345902.1"
                     /translation="MFKKLLELRQQKAAIEAEMRSILTKADEEKRSTTEAEGAKFDEL
                     RSQAENLVKEIARYEAITDEQRNQPGQQVETANVKVSDDELRHWIKTGELRSLATNTN
                     GGADGGYSVIPELDKQVMKRLTDDSVMRQIANVVKLTQGKEYKKLVSAGGAAVNHIGE
                     GEARTETATPKMNEVTIAVHNIYAYPKTTQEILDFGGVDILGWLTEEISESFVETEET
                     DLTNGTGTKQAKGFLAYPRTTANDKTRPFGTLQKMEVQPATVIGWNSKGQPETLTPYP
                     PYSVNVQRTVNRQNWLTAYIPHSYDENVAETPHFDQWLNFVSDGKQDKAKNILAALYA
                     ILTNRYNWQVFFQVTGKGGSGKSVFAGIATLLAGEKNTASARLENFDDERKIAGLEDK
                     KLIICSEQTKYAGDGGGLKAITGGDMVRVDPKNKHPFNARIAAMIMIVNNDPCKWTER
                     NGGIDRRIVNFRFNKRPPENERDPYFMDKITLEIGGIIRKVLDTFPDPLEAKRALEEQ
                     KESLEALEIKKQSDPLTDFFEYLYTTENTDGLYIGTGNTLGHDKIRTHLYPAYLAFVK
                     AKNVLELGLNTFVVGIEQAVKQHGNKHDFTKRKTNKGQRTNVHFKNFDDFQSDILN"
     gene            complement(16758..17153)
                     /locus_tag="PXB27_001102"
     CDS             complement(16758..17153)
                     /locus_tag="PXB27_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5345903.1"
                     /translation="MISNEAIRQEVIDLLQGNIQGIQHFHNGRAVFTDVENELPAIAV
                     FIDEAEYSQLTLCDGECNAYLKIGIYLPMFSTENDLDLVAQKISDVMNQATFHSMDEC
                     VLAKYSYDYDANESAWKTATLHYNINYLN"
BASE COUNT         5342 a         3600 c         3360 g         4981 t
ORIGIN      
        1 actgttttgc tattatatct ttttaagatg aaatgctcgt cttttgtttc actttttata
       61 ctgcttttta tttttatacc atttccagcc taaaaataga ataacaatgc ctaaaatggc
      121 aaaaatgagg tgctgcccat tagctacttg ttcttttagc caatccaaat tagatgcccc
      181 atagtcgcct aaataaaccc aaattggcac agagataata gcggcacaga aatcaactaa
      241 taaaaatttt acgaagctga cttttctggt aatacctgaa actaagtaaa ccaccgcgcg
      301 caagccaggt aaaaaacgag ccataaaaag tagtttccat ccttgttttt caaaacgttc
      361 ttgcacggtt gcaaaacgtt caggtgtcgc aacattcctg attaatggaa atttgagaat
      421 tttttcgcca tagattctac ctagccagta cattgtgcta tcgccaacta atactcctaa
      481 catactaaca accagcatcc aatgcacatt ggtgtagcct aatcctgaaa taacaccacc
      541 ggatactaac gtaatatctt caggaatcgg cacaccgaaa ccacaggcaa gtagcacaag
      601 aaaaacagcc caataaccat agctactaaa aaattcaatc aatgtttcca tattactcct
      661 taatgtttaa attccgaacc gatttacgtt tgaccaattc aggaaagaac tctaatactt
      721 taggctcttt tgcgttttcg ttatcttcaa tacgttctaa taataattct aatgctgccg
      781 agcctaagcg agattttgac tgatggattg tcgtaagagg cggtgcataa aagcgtgagc
      841 tatgcacgtt atcatacccg atcactgaaa tatcttgcgg cacattcagc cctttttcgg
      901 taatagctga aattgcccct agtgccattg agtcattaaa acaaaaaacc gcggttggta
      961 atttttcact gctcttaagc aaattattca tacactcata ccccccctca ggttcaaaat
     1021 cgccttcttg aatccattct tcacgaatag acaaacccgc ctcagatagc gcttttttaa
     1081 aaccttcaaa acgggttctt gcaatttctt tatctctatt gccggtaatt acagcaatat
     1141 cttgatgccc gttttcaatt aaatgctgag tcgcaatata gccaccttca acaccgtgat
     1201 ctaaaataat atcactgcgt tcatcacttc gtccccaatc cataatcacc attgggatat
     1261 tgctgttttc aaagatatca aatgaattag ctgtatattc ggagcacatc accaaaatgc
     1321 catcaacccg ttttttgatg agcatatcta aatgattttg aattttctca agtttgtttt
     1381 gagtattgca caaaaatagt gaataccctt tgctatagca aaattcctct accgctaaca
     1441 caatttcagc gaaaaacggt gattcgctgg ttgtaatgat cataccgata gatttagtcg
     1501 tattaatttt caaactccgc gctacagcac taggagaata gttcagcgct ttaatagcct
     1561 cccaaactgc ttttgtagtg tcctctgcta caaagcgggt tttgtttata acgtgagaaa
     1621 ccgttgtagt cgatacacca gcatattttg cgacatcttt tatcgttgcc ataccttttt
     1681 cctcctaaat aagtagtggt gcattataac tgattctaac tgacttttat tataaagaaa
     1741 tagatagaat gcaagcggtt gctttttatc aagaatttgc aaaaaatttt acaaatatca
     1801 ccgcttgtta ggctttaatt gcaatcattg agccaaaatt aaagcattga aaccaaagtt
     1861 caacttgtgt aaaaccagct tcttgcagac gttttttatg ggtttcaata ctatctgttc
     1921 gcatcacatt ttccaaagca ttacgctttt ggctgacttc caattcactg tagccatttg
     1981 ctcgtttgaa tgtgtgatga aggtcgatca atagctcgtt catttcctca ctctcaaagg
     2041 taaatttttc cgataacact aaaatgccat tggaatttaa tccttgataa atcttctgta
     2101 ataatgctag gcgatcttct cttggtaaaa attgcaaggt gaaattaagt accaccattg
     2161 aagcattttt aatttcaatg tggcgaatat catcaagtaa aatcgtcacc gggatctcag
     2221 agtgataagc acttaaatgg gttttacaac gctcaaccat cggctgagaa ttatctaccc
     2281 cgataatctg cacattcttc gcattcacat tacggcgaat agataaaatc cctgcgcctc
     2341 gtgaacaacc taaatcataa acatttgaat tatctgtgac aaaacgctcc gccaacattc
     2401 caatagcagt aataatattg gaatagcccg gcacggagcg ttggatcata tccggaaaca
     2461 cttcagcaac cgattcatca aaagtaaaat cgcccaattt ctcaatcgga gcggaaaata
     2521 atgtatctct ttgcattttt ctcccttatt tttctaaaaa tttattgatt gctctcacaa
     2581 cagttcctgt tttttctgcg tgaacccaat gatcagcgtt tgccacaatg aaagatttgg
     2641 cctgcggaaa ttgtgccaaa atcgtttgtg tatgctctgc ttgaatataa tccgataatg
     2701 cacctttaat aaacaaggtc ggtttatcca caaatacctg ctgccacccc atcacattct
     2761 gataattatc ttttagcgcc tgtaaattaa aacggaaaga ttgctcgctt tgggcatcaa
     2821 aagatttcaa cataaactgt tgcacgcctt catctcgaat atattcagcc attatcgctt
     2881 tagcttgctg acgattctgg ggtttagctt ctttcaccgc aaataaggct gccataacct
     2941 gctgatgccg attttgggta taagtgaccg gggcaatatc aatcactacc aatttctcca
     3001 ccagttcagg agcaaatgca gccaatgcca tcgcactttt tccgcccatt gaatgcccaa
     3061 ccacaatagc attttggata tttaattctg tgagtaattg tgctaaatct tgtgcaatca
     3121 aagcataatt catttcgtct gaatgaaaag atcgcccgtg atttctgaga tcaaccatta
     3181 aaatggagta acgttctgcg aatggttttg ctattgcgcc taggttattt aaatctccaa
     3241 acaatccatg taagaagatc atcgtctgag cattagcatg attattcgaa ggttggtaat
     3301 taaagtttaa ctgcgatttt tctgtcatat aaatgtcatt gttcatataa attttcagcg
     3361 atgttcctct ataaaaaaca atagaaatgc gtataatagc aagcactatt aacgcattta
     3421 atacccgcag atatttgagg atcccgatga aacgaatcga aattgatgat gaattatatc
     3481 agtatattgc aagccgtaca caatctattg gtgaaactgc gtcagatata ttacgccgtc
     3541 tattacgcct gccacaatcg cctcagcctt ttgttttagt acaagaacac atgatcaacg
     3601 agcttaaaga gcttgtcaaa acgccttctc gtgctacgaa aaaaaatgaa tccaatgcag
     3661 aaaaaaccgt tgaaaaattg gaaaacattt taaattccga gcattttatg aacgaaaata
     3721 aaaatgtagt gcgttttact atgctattag ccgcattata tcgagcaaat ccggaggctt
     3781 ttgctaaagc aaccgaaaaa gtgcgtggta atgaacgtat ctacttttcg caaagtgaag
     3841 ccgaaatttt agctactggg agtggagtaa aagccaagca gatcccagat tcaccgtttt
     3901 gggtaattac caataataat accgcacgca agggacttat tctcaaaggc gtaatggaat
     3961 ccatgaaagt accaagccag ttaatcgaac gcattcaagc gctttttatc taattaattg
     4021 aaatgctcat caattctact cgctttccta cccaattttg ggctgctcat tcagcccaaa
     4081 atgtggcgat tatttgggaa aaaggaacat cagactatct taaccacctt ccatcaaaca
     4141 tcacttggta tagtttgaat cagttgattg cgcaagctat tttcgtgttg caacaacaag
     4201 gtgctactcc gcacagtttg attgcctatt gtggttctca tcggctcatt ggcttgctca
     4261 cttattgtgc tagccttgct ttgggggcta agattctaat gcttaaccct gctcaaacag
     4321 caagtcaacg acaagcagtc ttagatgaca atggtgttga gctcattatt aatgatgccg
     4381 actttgcaaa attttcagca aaaacgaccg cttgtaatgc cttccctacg attgattttg
     4441 aaaaaccagc taccctaacc ctaacctcag gctcaagcgg taaacctaaa gcagtggtgc
     4501 attctatagc ggcccatctc tatagtgccg aaggagtatg tgaattaatg gcatttgagc
     4561 agccaaactc ttggctactg agcttgcctc tgtttcacgt ttccggacaa ggcattattt
     4621 ggcgttggtt atttaaaggt gcaacactgt atgttcacga agacaaaagt gatttttttc
     4681 tttcagcaca aaaagtgagc catctctctt tagtccccac ccaattacaa cgctatttaa
     4741 accaattaga ttctatggtc agccaaaaat gtttactggg tggatctatg cttccagccg
     4801 aattgattgc tcaagcacaa caatctggga tgactacctt ttcaggctac ggaatgactg
     4861 agatggcatc aaccatttgt gcagtagaaa atgatttaaa cagtgttggt tttccgttaa
     4921 aacatcggga actaaaatta gaaaatggtg aaatttgggt aaaaggcaaa tcactcggct
     4981 taggctattg gcaaaaaaat gggcaaattc gaccgcttgt aaacacacaa ggctggtttg
     5041 ccactaaaga tcgtggcgaa tggaatcaca aaaaacagct tgtaataaaa ggacgattgg
     5101 ataatatgtt tatttcagga ggggaaaata tccaacctga ggaaattgaa caactgattt
     5161 ttcgttctca gcttgtcgaa cagattatcg tgttgccgat tgaagataag caatttggac
     5221 atcgaccagt ggcgttctta caattcaagc aatctgattc aaaaaatgaa ctccataaat
     5281 taaaaatttg gctgtctgat aaattagaaa gattcaaaca gcctgttgcc tattatttac
     5341 tggatataga aaaataccaa aaacaaggct taattaaact atcaagaaca caattacaac
     5401 aagatttaaa actcaaagta ttaaaggaaa tctatgttta gaccgatttt aaaaacattt
     5461 gtgcttagtt taggcttaat gctaaatagt tatgcattag ctgaattgcc aacccaacag
     5521 gcagtgcaaa cgcagttaga tagcgccaaa aaattagatc aagccgatgc ggataataag
     5581 gcattggttc aaacacttga agatacgctt gctttcttag cccaaattga taaacaaaaa
     5641 gcagataatc aggcattgaa taaatctatt caagattccc aaaaagcatt aaaaacaagc
     5701 caacataatc ttgaaaaatt aaaagaggtg actattgcaa gtgtcgataa tttaacacaa
     5761 aaagcgacaa cagatttaca aaaagagtta acctctacac aatctcagct ggaaacagta
     5821 caacacgatt taagtattat taataataat ttagttgcac aaaataccgc accggataag
     5881 gctcaaacgg ccttaaccga gatggttaaa cgtaaacagg aaattagtag ccaactttca
     5941 gcgactaaca ttaaaaatga actacagaca aagttagaag tagaattaga attaatagaa
     6001 ttaaaaaaca cttacaatca aatattactt ggtggcagcg aggatttatc tgcgctttac
     6061 tctgctcaat tagatgaaaa aaagcaagag cagcaaaatc tacaagcaga aattgcaaac
     6121 ctgcagactg ctatcaacaa taaaattgta gaagagtctc agaacaagct agatcaagca
     6181 caagcggttc aaaatcagca aaataatgca acgacgaacc cagttatctt gcgtgaattg
     6241 gatataaata ccaaagtcac acaagagttg ctaaaacaga ccaaagatat gacgcagtta
     6301 tcacaagata atttgcgaat caaaagtgtg ctagataact tacagcaaac ccaacgtaat
     6361 attgaagaac aaatcagttc tctgcaaggt acgttggtgc tttctcgtat tattaataag
     6421 caaaaacaat cattaccaca agatgaaatg atttccggac tatcaaaaca aattgcagat
     6481 ttacgtgtac gtgtttttga tatcactgaa tttaaagact cttttgcgga cataaatgct
     6541 tatatcagcc gcatagagca agatgaaaaa acaaccttta cgagtaaaga gaaagaacaa
     6601 ctgagtaaaa ttttacagga acgttctgac actttaactg agatgattaa atcattaaat
     6661 aatcagctca atttattaat caatattgaa ctaaaccagc aacaagtgca gaccattagt
     6721 gatgcgttac agcaaaaact gcaacagcaa agcttctggg ttaaaagtaa caaccccatt
     6781 gatcttgaat ggtttagtaa atttgtcgat attacccact atcaatttat tgatattgga
     6841 aaaaaactca atttttcaaa ttggcgggat aatttattac ctgcaagtat tttagtcgga
     6901 attttattgc tattagccat ctttattaat cgccaaaaag ataaaattaa acaaaaatta
     6961 caccaaatta ataatcgact aaaaattgtc gccacagata gccaatggaa cacccctctt
     7021 gctatttttt ggacattagt gctttgcctg ccaagtacac taatgttctt agccggattt
     7081 gttgctatca cttatatttc atttgaaaat cctcaggaag tctggacttg gggattaaaa
     7141 atggcaattt attggctgta ttttgccttt ttagtggcga tgctacgccc gcacggcata
     7201 ggacatattc actttaatat gccacaaaaa agtaatgaaa tcttccgtaa tattttaaaa
     7261 cgttcggtat ggatcattgc tttattactg aacacctcta tttttacaaa cttagagatg
     7321 ggaatatctt acgacatgct tggacaaaca atgactattt ttgtccttat attaatgatt
     7381 tggatgattg taccggcatt taggcaagct atttctacct atgagaatgt cacaacagaa
     7441 gagaaaaaag aatctaaaag tattttactc actattgtca aattagtgct actgcttgcc
     7501 cctatcattc ttatcattct gattgcattg ggctattact atactgcact ggtgatcatt
     7561 gagcatttag tagattctta ttttgcgatc attacttgga ttattattcg taatattctc
     7621 tatcgagcat tctctatttc atcacgccgt ctggcttaca gacgtttaca agaaaaaaga
     7681 gaagcagctg aagccaaaac acgagctaaa gcagaacaaa atgatgaaaa cccggacggt
     7741 gtcgagcttc cttttgagct aaaagaagac tctattgccg taagcgaagt caagaaccaa
     7801 atgttgaaat taactgattt tgtgctttgg attggcttat ttgtgctgct ttactgggtt
     7861 tggtcggact taattactgt tgcactctac ctagacggcg ttaccttatg gcaacaaagt
     7921 gttacaacag aagccgggat tgtaatggaa tccattaccc tgctgaattt actcactgct
     7981 attctgatta ttgtggtaat ggtggtactc attcggaact tatccggttt acttgaagcc
     8041 ctgattttct cgcacttcaa gttatcgcaa ggtacaccat acacaattac cacactctta
     8101 acctatttga ttattgcgtt aggggcaatc tttgcgttcg gtacattagg gatgtcttgg
     8161 agcaagctcc aatggctact aactgcactt tctgtcggtc tgggttttgg tatgcaagag
     8221 atctttgcaa acttcgtttc agggattatc atcttgtttg aacgtccaat gcggattggc
     8281 gacaccatta ccatcggcac ttacagcggt acggtttcca aaattcgtat tcgtgccaca
     8341 accttagtcg attttgacgg caaagaagtg attgtaccaa acaaagcatt cgtaaccgaa
     8401 cgcttggtga actgggcatt aagcagcacc gcaacccgca ttattgttca agtcggcgtg
     8461 gcttacgggt ctgatttaga attagtcaaa cggctgttat tacaagtggc ggaagaaaat
     8521 ccgaaagtct tgaaagatcc acaaccaagg gcttatttcc ttacctttgg tgcaagcact
     8581 ttagatcacg aattaagagc ctatgtgaac gatttatctg atcgtaaccc aactattgat
     8641 gcactaaata cacgcatcca ccagctcttt aatgaacata atattgatat tgcatttaac
     8701 caattagatg tttatatcaa aaacaacgat acacaagatg aaattaaagt agattcaaaa
     8761 cgctttgagt ttgaacgacc aaataacata acagcaaaag aaaaggaata acaatggcag
     8821 gcaacagcat cggacaatta tttaaagtga ccactttcgg cgaatctcac ggtattgcat
     8881 taggctgtat cgtagatggc gtaccgccga atatggcttt aagcgaagca gatttacagc
     8941 cggatttaga ccgccgtaaa cccggtactt cacgctacac cacgcctcgc cgtgaagatg
     9001 atgaagtgca aattttatcg ggagtctttg aaggcaaaac gacgggaaca agcattggtt
     9061 taatcattaa aaacggtgac caacgctcac aagactatgg cgacattgcc gaaaaattcc
     9121 gcccggggca cgcagattat acctatcagc aaaaatacgg cattcgagat taccgtggcg
     9181 gtgggcgttc ttctgctcgg gaaaccgcaa tgcgggttgc cgccggggcg attgccaaaa
     9241 aatatttgcg tgagcaattt ggcattgaag tgcgaggcta cctctcacaa atcggcaata
     9301 tcaaaattga cccgaaaacc gttgaaaata ttgaacaaat tgattggcac gcagtcaata
     9361 gtaatccttt cttctgcccc gatcccgttg cgattgaagg ctttgatgcc ttaattcgtg
     9421 agctgaaaaa agagggcgat tcggtggggg caaaattaac cgtgattgca gagcgtgtac
     9481 ctgtagggtt aggcgaacct gtgtttgacc gcctagatgc cgaccttgcc cacgccttaa
     9541 tgtcgatcaa cgccgtgaaa gcggtggaaa ttggcgatgg ttttgcagtt gtggaacaac
     9601 gtggtagcca gcaccgagat gagatgacgc cgcaaggctt tctctctaat cacgccggcg
     9661 gaattttagg tggtattagc tccggtcagc cgattatcgc tcatattgcg ttaaagccga
     9721 cctcaagcat tactattccc ggcagaagcg tgaacaaaaa caacgaagcc gttgaagtga
     9781 tcactaaagg cagacacgac ccctgtgtag gtattcgtgc tgtgccgatt gcagaagcga
     9841 tgatggcgat tgtactactc gaccatctgt taagatttaa ggctcaatgc cgttaaacac
     9901 ataaaattgg aaaaatgaaa ttattaaaaa cacttttagt ctcaagcctt gcttttacag
     9961 ctaccgcact gaatgcaacg caatgggaga aaatcaaaac tccggttcaa ggaaaagcac
    10021 aatctatcgg cggttatagt aacggctgta ttattggggc acagcccctt gcattaaaag
    10081 gcgaaggcta ccaagtgatc cgctcggctc gtaaccgcta ttacggacac ccacaactgc
    10141 tcgattatct cactcactta ggcaaaaaaa ccaaagctgc cggtgtgccg accattttaa
    10201 tcggcgatat gggaatgcct gccggtgggc gttttgcctc cggtcacgcc tcccatcaaa
    10261 ccgggctgga tgctgatatt tggtttaagt tcggaccgat ggacgatgaa accgcccgca
    10321 atccagcagg gcttgccacc attatggtaa accgtagtac cgagcgggtt gatgattcac
    10381 tctggaatca acaccatact aatttagtaa aacttgctgc aaccgatcct cgagtggatc
    10441 gcatttttgt caatcctgcc attaaagtga aactctgcca aaccgcaggc aatgatcgtg
    10501 cttggttacg caaaattcgc ccgtggtacg gacacgactc ccatttccac gtccgtttaa
    10561 cctgcccggc tgatgccgca agttgtgaaa atcaagccct tgtgcctgcc ggtgacggct
    10621 gtggtgaaga gctttactcg tggtttgagc cgaaagaagc ctccggcaca ccaaaagcca
    10681 aagtgctacc cacagcgcct gttcaatgtc agatgatttt atctgaacaa ggcttgaatt
    10741 aattgcaaaa taacaacctt ttttgaccgc ttgtcagccc acaagcggtt cattttctta
    10801 aataatttac gattatgcgt ttatttgttg ccgaaaaacc cagccttgcc cgagcgattg
    10861 ccgatgtgtt accaaaaccc cataccaaag gagatggctt tatccaatgc ggcgaacacg
    10921 acactgttac ttggtgtatc ggtcatttat tggaacaggc tgaacccgat gtctatgatg
    10981 agcgtttcaa aatgtggcga atggaacatt tgccgattgt gcctgaacaa tggaaattga
    11041 ttcccaaaaa agagacgctc aagcagttta aagtggtgga aaagctgatt aaaaaagccg
    11101 atgttttagt caatgccgga gacccggatc gagaagggca gctgttggtg gacgaagttt
    11161 ttggctattt gaatttatct gctgaacgca aaagccaaat tcagcgttgt ttggtaagcg
    11221 atctcaaccc gaatgcggtg agccgagcca ttgaaaaatt gcaaaaaaac agcgatttta
    11281 taccgcttgc aacctctgct ctcgcccgag ccagagcgga ttggctgtat ggtatcaata
    11341 tgacccgagc ctatacccta caggggcgtt gggcaggcta taaaggcgtg ttatcagtcg
    11401 gacgtgtgca aacgccggta ttagggctga ttgttcgccg agatctggag atcgagcatt
    11461 tcgtgccaaa ggattatttt gaggtgttgg cacagattgt cgtgccggaa acccaagagc
    11521 gatttaaggc gcagtggcaa ccgagcaaag cctgtgaaga ttatcaagat gaagatgggc
    11581 gggtgctgtc tcgtccattg gcagaaaatg tggtaaaacg gattaccgac cagccggcaa
    11641 ccgtcaccga atatcaaaat aaaattgaaa aagaaactgc accgctgcct tattctcttt
    11701 cggtattaca aatggaagcc gccggcagat atgggctatc cgcccaagcg gtgttggata
    11761 tttgccaaaa gctgtatgaa acccataaac taattaccta ccctcgttct gataatcggt
    11821 atttgccgac cgaacatttc aacgacagat ttaaagtgat ggcggcgatt gctcatcatt
    11881 tacctgaata tcaacaaaaa ccggaagtga ttgatgccac tattcgcaat cgttgctgga
    11941 atgataaaga ggtggaagcc caccacgcca ttattcccac tgcacggcaa gggacggtac
    12001 atttaaccga aaatgaaaaa agagtttatc aactcattgc ccgccaatat ctaatgcaat
    12061 tctgccctga tgcggaatat cgcaaaagta aaatcgggct tgaaattgca ggtggtagtt
    12121 ttgtcgctca agcacgcaac cttgtaattg ccggctggcg agcgttactg ggtaaagaag
    12181 atagccttga cgatgcagag ccactactac cgattgtcaa aaaagggcaa atcctccatt
    12241 gtgaaaaagg ggaaattgtc agcaaaaaaa cacaaccgcc ccgccacttt actgatcgca
    12301 gtttgctttc agcaatgacc gggatcgccc gttttgtaca agataaagaa ttgaaaaaaa
    12361 tcttgcgtga aaccgatggt ttaggcacgg aggcaacccg agccggtatt atcgaattgc
    12421 tgtttaaacg tggtttttta atccgtaaag ggaaaaatat ccacagtacc gaagccggca
    12481 gaatattgat tcaagcactt cccgactctg ctacccagcc ggatatgacc gctcattggg
    12541 aaatgcagct gaatcaaatc agcaaaaaag agaccagcta tcagcaattt atgtttgatc
    12601 taaaccaaca gctcccacat ttattacaaa gcccaaaccg ccaagtgttg caaaatttag
    12661 cgaaaatcac accgcttgca agcggcaata aacctcgtta ttttaaaaag cgaacacaaa
    12721 ctgctgaaaa ttaaatcaag caggtaaaaa ttactgttta gcacttgtca aacgggcgta
    12781 aattctatat tatgcacgcc attgcaaatt tcgcataaag aatacaagag aataaattaa
    12841 tgttaattaa tgtactcact attgtttatc ttgtagttgc cgtattactg atcgcatttg
    12901 tattaatgca acaaggtaaa ggcgctgatg caggtgcttc tttcggggcg ggtgcttcag
    12961 caacagtttt cggctcagca ggatcagcaa attttttatc aagaacaact gctctacttg
    13021 caacgatttt cttcgcaatt agtttagtta tcggtaattt aagctcacac aatgtagcac
    13081 cgaaaagcca atttgaagac ttaagccaag ttgaacaaaa gcaagtagaa cctgtaaaaa
    13141 tagaaaatag cgatatccct caataatcgc ttaacattaa aaagaataaa gctctggtgg
    13201 tggaattggt agacacgcca tcttgagggg gtggtggaca cagtccgtgc gagttcaagt
    13261 ctcgcccaga gcaccattca actaaatttg gctcaacgga gctaaactaa aaaagcctta
    13321 gaaagcctgt aaaatcaaca tttacaggct tttttactat cttaactcaa cggctctaaa
    13381 cgtttcctat cacaaatttt agttacacgt ttagttacac gttttaaatc gtgttactat
    13441 actttcaata atcgtgtaac acttcgtagg tttaatttat ggctagaaca actaaagcat
    13501 taaataaaac tcaaatagaa aatgccaaat caaaggataa agaatatatt ttatccgatg
    13561 gcgatggatt aaatcttaga gttaaacctc tccctaaagg ctctaaaatt tggattttta
    13621 actacactaa cccacaaact aaaaacagaa caaatttaac tattgggcgt taccccttaa
    13681 ttacacttgc ggaagctcgc actatatgtg atggctaccg ctcgttaata gagaaaggca
    13741 tagatccaca ggaagaaaaa aagcggctgc aacaagaagc agaaaatcga gtaaatgata
    13801 ctttccggaa tgtggccgaa agctatttca atggtattta taaagagaaa gcaaaaaatc
    13861 cgattactcg tgaaaagaat tggataaggt tagaaaatca tattttcccc tatatcgggg
    13921 ataaacacgt tgaagaaatc aaagtaaaag cccttgttga tatttattct aaagtggcag
    13981 ataaaagcaa cacattaaaa aaactgcatc agcttgtatc tgctgtaatg gatcacgcca
    14041 ttacgcaagg cattatagaa agccataact gccgattagc agttaaaaac ttttatatca
    14101 agccatctac gccaaatccc actattgagc ttgaagaatt accgcagtta tttactgatt
    14161 tacataatgc gaagttatca aagcaaactt acgcccttgt ttgttggtcg ttcttgactg
    14221 ctctacgccc aaatgaagcc gttaatgctg aatggaaaga aatagacttt gagaaaaagc
    14281 tatggaatat ccctaaagag aagatgaaag gaaaagcaga taaaaaacgc cctcactctg
    14341 tgccgttatc ctcccaagcg ttaaagatat tagagatgat gaaaatgcac tctaattcta
    14401 gcccttttgt ttttccaggt cgttcatcaa atagtaaacc aatgagcaac gcaacggtaa
    14461 attcaacatt aaaaaggaat ggatataaag atagactaac cgcacacggt attcgggcat
    14521 ttgtgaaaac atttctatct tctcgcaaag tagatcgcca cgtttccgaa agtatattat
    14581 cccatttatt agaaggtgga gatgatttag aaaacacata caaccgatat gattttttag
    14641 aagagcgtgt gccagtaatg cagcttttag gtgattattg cgaacagtgc ggaatgaatt
    14701 tagagttagt tcggggttag ggaggtaaaa tatatgattt tagtcgtcac gtcatcacct
    14761 ttcccacttt cccctttaaa atcaaatagt taagtggtga tgactggttt taaagtcatc
    14821 attaagtcat cacaaaaagg tgatgaccta gaaaaaaaga ggggtttccc cctctttgct
    14881 aattcaaaat atcactttga aagtcatcaa aatttttgaa atgaacgttt gttcgttgcc
    14941 ctttattcgt ctttcgtttt gtgaaatcgt gcttattgcc gtgttgcttt actgcttgct
    15001 caatacctac tacaaaagta tttaagccta gttctagcac attcttagcc ttaacaaagg
    15061 ctaaatatgc cggataaagg tgcgttctta ttttgtcgtg tccaagcgtg ttgccagttc
    15121 cgatataaag cccgtctgta ttttctgttg tatatagata ctcgaaaaaa tccgttaatg
    15181 ggtcagactg ctttttaatc tccaacgcct ctaggctctc tttttgctcc tctaaggctc
    15241 ttttagcttc gagtgggtca gggaaggtat caagcacttt acggataatg ccccctatct
    15301 ctaatgtaat tttatccata aaatacggat cacgttcatt ttcaggcggt cttttattaa
    15361 aacggaaatt gacaatccgc ctatctatac cgccattccg ttctgtccat ttacaagggt
    15421 cgttattgac gatcataatc attgccgcaa ttcttgcgtt aaatgggtgc ttgttcttcg
    15481 gatcgactcg caccatatca ccgccagtaa tcgctttcaa tccgccacca tctccagcgt
    15541 atttcgtttg ctcggaacag ataatcagct ttttatcttc cagccctgcg attttgcgtt
    15601 catcatcaaa gttttctagc cttgctgaag ctgtgttctt ttctccagct aatagtgtcg
    15661 caatgccagc aaataccgat ttaccgcttc cgcctttccc tgttacttgg aagaatactt
    15721 gccagttgta gcggtttgtt aaaatcgcat agagagcggc tagaatgttc ttcgccttgt
    15781 cttgcttgcc gtctgataca aagttaagcc attgatcaaa gtgtggtgtt tcggctacgt
    15841 tctcgtcata gctatgtgga atataagccg ttagccagtt ttggcggtta acggttcttt
    15901 gaacatttac cgaataaggt gggtaagggg ttaaagtttc gggttgcccc ttgctattcc
    15961 aaccaatcac cgtagccggt tgtacttcca ttttttgaag cgtgccgaat ggtcgggttt
    16021 tatcgttggc tgtcgtgcgt ggataagcta agaagccttt agcttgtttt gtgcctgtac
    16081 cgttggttaa atcggtttct tcagtttcga caaaggattc tgaaatttct tcagtcagcc
    16141 agcctagaat atctacacca ccaaagtcta aaatctcttg cgtggttttc gggtaagcgt
    16201 aaatattatg aacggcaatc gttacttcat tcattttcgg ggtggcggtt tcggtgcgtg
    16261 cttcgccttc accgatatga tttaccgctg caccgccagc cgatactaat tttttatatt
    16321 ctttgccttg ggtcagtttc accacgttag caatttgtcg cataacagaa tcatctgtta
    16381 ggcgtttcat tacttgcttg tcaagttccg gaatcactga atagccaccg tctgcccctc
    16441 catttgtatt cgtagcaagc gaacgcaatt cgcctgtttt aatccagtgg cgaagctcgt
    16501 catcagatac tttcacattt gcggtttcaa cctgttgccc cggttggtta cgctgttcat
    16561 cagtaatcgc ttcataacgt gcgatttctt ttactaaatt ttcagcttgt gagcggagtt
    16621 catcaaactt agccccttct gcttcagttg ttgagcgttt ttcttcgtct gctttggtta
    16681 aaattgaacg catttctgct tcaatagccg ctttttgttg gcggagttct aaaagttttt
    16741 taaacattgc cttatcctta attcaaataa ttgatattgt agtgaagcgt tgctgttttc
    16801 cacgctgatt cgttagcatc ataatcatag ctatacttag ccagcacaca ttcgtccata
    16861 ctgtggaatg tggcttgatt catcacatca ctgatttttt gagccactaa atctaggtcg
    16921 ttttcggttg aaaacattgg taagtaaatg ccgattttca aataggcgtt gcattcgcca
    16981 tcgcaaagcg tgagttggct atattccgct tcatcaatga atacggcgat agccggtaat
    17041 tcattttcta catcggtgaa tacggctcgg ccgttatgga aatgttgaat gccttgaatg
    17101 ttaccttgta ataagtcgat aacttcttgg cggattgctt cgttagaaat cataaaaaag
    17161 tcctcttaat taagaaggct acaattagcc acagaacaat ataggcataa tttcaacgga
    17221 gtaaataggg taaaatttaa tcgtttagct acgtttgaac acgttaagag gataattttt
    17281 aaa
//
LOCUS       DANLUQ010000043        16965 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.043, whole genome shotgun sequence.
ACCESSION   DANLUQ010000043 DANLUQ010000000
VERSION     DANLUQ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16965
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.043"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            110..2719
                     /gene="adhE"
                     /locus_tag="PXB27_001103"
                     /gene_synonym="adhC"
     CDS             110..2719
                     /gene="adhE"
                     /locus_tag="PXB27_001103"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDL5345904.1"
                     /translation="MAKNTQPFDAQAEVNELVEKGLKALDEFRKLNQEQVDFIVAKAS
                     VAALDKHGILAMHAYEETGRGVFEDKATKNLFACEYVVNNMRHLKTAGVISEDDVTGI
                     TEIADPVGVVCGITPTTNPTSTTIFKALIALKTRNPIVFAFHPSAQQSSAHAAQIVRD
                     AAVAAGAPENCIQWITQPSMEGTSALMKHPGIATILATGGNAMVEAAYSCGKPALGVG
                     AGNVPAYVEKTAKLKQAVYDIVMSKSFDNGMICASEQAAIVDKEIYAEFIKEMQSYGV
                     YLVNKKEKALLEKYIFGIDKAKDENCSGAKLNSAVVGKPAAWIAEQAGFKVPEKTNIL
                     LAECAFVGEAEPLTREKLSPVLALLKSDSTEHGLELSEAMVNFHGLGHSAAIHTENKA
                     LAKTFGERVKAIRVIWNSPSTFGGIGDVYNSFLPSLTLGCGSYGKNSVGNNVSAVNLI
                     NIKRVGRRRNNMQWFKVPSKIYFERDSIQYLKSMHDVHKVMIVTDRSMVDLGFVDRIT
                     EQLRQRRNQVMIQFFTDVEPNPSLQTVQRGTELMRSFQPDTIIALGGGSPMDAAKIMW
                     LFYEQPEVDFRDLVQKFMDIRKRAFKFPQLGRKAKFVGIPTTSGTGSEVTPFAVITDG
                     DIKYPLADYSLTPTIAIVDPALVMSVPSHVAADTGLDVLTHATEAYTSILANDYTDGL
                     ALQAIKLVFENLEKSVKEFDETAREKMHNASTMAGMAFANAFLGICHSMAHKIGGKFH
                     TIHGRTNAILLPHVIRYNGTRPTKVATWPKYTNYVADVRFQDIARMLGLPAETPEQAV
                     EAYAQAVHDLAVRCGVKMSLREQGVDEQAFLNARRELALHAFEDQCTPANPRLAMIED
                     MEAIMTKAYYGKE"
     gene            2866..3762
                     /locus_tag="PXB27_001104"
     CDS             2866..3762
                     /locus_tag="PXB27_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HDL5345905.1"
                     /translation="MQITILDGGISRELMRRNAPFHQPEWSAAALYEGPHFVQAVHED
                     FIAHGAEVITTDSYAVVPFHIGEKRFAADGKMLADLAGRLAKQAVKNSGNIATQIAGS
                     LPPMFGSYRADLIQMERFAEVAQPLIDGLSPYVDIWLCETQSAIIEPISIKALLPKDN
                     RPLWVSFTLTDDEPTTEPQLRSGESVKQAVEEMVKLGVQAILFNCCQPEVIGEALSVT
                     QKTLAELKATHIRTGAYANAFAPQPKDATANDGLDEVRQDLTLEAYLGWAKKWTEQGA
                     TIIGGCCGIGVEYIETLAKNLK"
     gene            3772..5181
                     /locus_tag="PXB27_001105"
     CDS             3772..5181
                     /locus_tag="PXB27_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL5345906.1"
                     /translation="MSNKKIGLISLTALVLSSMIGSGIFSLPQNMAAVAGAEAITIGW
                     LITGVGIIFLGLSFFFISRLRPDLDGGIYTYAREGFGELMGFMSAWGYWLCATIGIVG
                     YLTVAFEGLGVFTDNPNTVIFGQGNTLASFIGSSIIVWLVHALIAGGIKEAAFVNLIA
                     TFVKVAPLILFIGLGIWYFDVDLFNSDVKATALNNSVSDQVKSTMLITLWVFTGVEGA
                     SVLSAHAKKRSDVGLATVLGIIIALALYVAITILSLGILPRETIANMANPSMGPLLDA
                     MMGPTGKVIITACLIVSVLASYISWTMYSAEVPYRGSKNGAFPKILDKVNKNGTPINS
                     LWFTGFIVQFCLVLVFVFEQSYNTLLLISTSMILIPYFLIGAYLFKLAIQTNATWYIK
                     LTGFMAALYGLWIVYAAGLEYLLLSVVLYVPGILLFLYSNYKFNGRLTLSTAEKVILI
                     VLLVIFGYAVIELPALLAA"
     gene            5288..6511
                     /locus_tag="PXB27_001106"
     CDS             5288..6511
                     /locus_tag="PXB27_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HDL5345907.1"
                     /translation="MQTYQHKALCRVRTITLFLSLTKDKLQWETALNAAKAEFDLLVP
                     AIQTQGYHIQSIRIVTNSFGEYLDLTNIETAKQDLAYLKQLLTQLNDSGLRIRFAIGE
                     AKTPHELALLPELILNYGDLSNACVNIEPDEFGILDNETILQSAQIIKQIAEITPRGE
                     GNFNFTVNFNCKPFIPYFPASYHSSKLPNSFVIGLETPDLLVAVLKELKASPHNDFYR
                     QCYETMSQALQYHVDEVLEAVKSVQHKLQFAFAGIDSSAAPSKNCASMTEVYELMGVP
                     FFGAAGTVEASSLLTKVFKSIKHVPLVGFSGLMLAVTEDLGLAAGTHKAQFDIRALLT
                     YSAVCGIGLDTVPISNEATVEQIAALMRDTGTMAFRLNKPLTVRLFPIPNKSAGEVTE
                     FESDDLCNCRILAVP"
     gene            complement(6549..7745)
                     /locus_tag="PXB27_001107"
     CDS             complement(6549..7745)
                     /locus_tag="PXB27_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL5345908.1"
                     /translation="MKQVIRFIVFSTLSLFSFSTFAKTIVAIDAGHGGKDPGAIGKNL
                     GILEKSVTLAISKELKALLDADPNFKAVMTRSGDYFIQLPQRTEIARKHRADLLVSIH
                     ADSSPRGDSLKGASVWVLSNSRASDEMGKWLEDHEKQSELLGGAGSVLSSNNERYLNQ
                     TVLDLQFSHSQRAGYELGKSILAKMSNVAALAKSSPQHASLSVLRSPDIPSVLVETGF
                     LSNSTEEAKLASANYRKQVARAIYNGLVAYRAKNAVASSSVSKKSDKEDKQNKKEEKT
                     AKADLTKKPASEKQEKNTQSDKTKLKEKSKKEDKKEDSAKTNKKKEQQLKEKTALEKD
                     QKNQSKKSDSQREIKVSNSGYHIVQQDETVYSIARAYGTTPQKISELNNLKNYQITVG
                     KKLKVK"
     gene            complement(7765..8256)
                     /gene="tsaE"
                     /locus_tag="PXB27_001108"
     CDS             complement(7765..8256)
                     /gene="tsaE"
                     /locus_tag="PXB27_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDL5345909.1"
                     /translation="MTDSLSFYCEDETKLLEFGQSFALELKKYLLQDQAHSLVVYLNG
                     ELGAGKTTLTRSIVRAFGHQGNVKSPTYTLVEEYQLPPFSLYHFDLYRLADPEELEFM
                     GIRDYFKPQTLCLLEWAVKGKGMIPEADFVIQIDYKNDGRQISLLPQNQTAVDILVNF
                     HQK"
     gene            complement(8256..8810)
                     /gene="orn"
                     /locus_tag="PXB27_001109"
     CDS             complement(8256..8810)
                     /gene="orn"
                     /locus_tag="PXB27_001109"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HDL5345910.1"
                     /translation="MAQNEQNLIWIDLEMTGLDPEKERIIEIATIVTDKDLNILAEGP
                     VLAVHQTDDLLEKMSDWCVKTHTANGLIERVKASKLTERAAELQTLDFLKRWVPKGAS
                     PICGNSIAQDKRFLYKYMPDLADYFHYRHLDVSTLKELARRWKPEILDKFSKGNTHLA
                     LDDIRESINELKFYREHFIQLDQK"
     gene            complement(8820..9299)
                     /gene="coaD"
                     /locus_tag="PXB27_001110"
     CDS             complement(8820..9299)
                     /gene="coaD"
                     /locus_tag="PXB27_001110"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDL5345911.1"
                     /translation="MSYNVIYAGTFDPITNGHLDIICKASHLFGKVIVAVAQNPSKQP
                     LFSLEERVELVKQSCTQWQNIEVIGFSGLLADFAKQQNAKALIRGIRGADDIDYEIQL
                     AQLNYKLSGDLETIFFPPSIEWRYLSSTMVREIYRHNGDISQFVPQAVKNALKIAKV"
     gene            complement(9301..10581)
                     /gene="waaA"
                     /locus_tag="PXB27_001111"
     CDS             complement(9301..10581)
                     /gene="waaA"
                     /locus_tag="PXB27_001111"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HDL5345912.1"
                     /translation="MLRLLYICLSYLLQPVVLLLMWYKGRKQPAYRKRLWERYGIYDE
                     SEKPKAKGVVIHAASVGEVIAATLLIKAVSKQYPELPLIVTTVTPTGSGRVQAAFGNS
                     VSHFYLPYDLPDAIERFLNFIDPKLMIVIETELWPNLIRKVNIRKIPFVIANARLSPR
                     SAKRYGWVKGSIKDMLNQISLIMAQDEVSRDRYLALGYAPAKMVNTGNLKFDLEITPQ
                     LHQSVIKTKASLNLTERPIWIAGSTHEGEEKLILDAHKLLLEHYPNLVLILVPRHPER
                     FGLVELLIQKSDLNYVKRTENKTLSQNTQVMLGDTMGEMMLLYGLSDIAFVGGSLVKH
                     GGHNPLEPIAFNIPVVSGLYTYNFPEIFEKLREVKGVIEIESSVEALTESIQLLLKQP
                     QIGQEIAKSGLSVLQENQGALKRHLDLLASYLEK"
     gene            complement(10761..12140)
                     /gene="yegQ"
                     /locus_tag="PXB27_001112"
     CDS             complement(10761..12140)
                     /gene="yegQ"
                     /locus_tag="PXB27_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HDL5345913.1"
                     /translation="MTLKYAKPELLSPAGSLKNMRYAFAYGADAIYAGQPRYSLRVRN
                     NEFNHANLKVAIDEAHALGKKFYVVVNIAPHNSKLKTFIKDLKVVVDMKPDALIMSDP
                     GLIMLVRENFPEMDIHLSVQANAVNWATVKFWRQMGLTRVILSRELSLEEIEEIREQV
                     PDMELEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYKMEEGKIDEVGQI
                     VPKYEPEIEVKNVAPTLGNGEATDKVFLYTESQRPDEQMTAFEDEHGTYFMNSKDLRA
                     VQHVERLTQMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDESLMDTLESL
                     AHRGYTEGFLRRHTHDEYQNYEYGYSISERQQFVGEFTGKRNAEGLAEVAVKNKFLVG
                     DSVEMMTPKGNIVFKIERMLNRKNENVDAALGDGHFVFLDVPADIELDFALLMRNLVN
                     TNTRNPHQA"
     gene            12268..12639
                     /locus_tag="PXB27_001113"
     CDS             12268..12639
                     /locus_tag="PXB27_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345914.1"
                     /translation="MKLKYLLATTLLAFSTFTAAQSIKTEMMQMNMQANKLMRAESAE
                     EFSEIANDFIIITEKTKGIFPNSVEGDKAAQEDYQAGMQKLIDMVNQASEKAQAGELQ
                     EAKMLISELEMIKREYHKKYK"
     gene            complement(12678..16154)
                     /gene="dnaE"
                     /locus_tag="PXB27_001114"
     CDS             complement(12678..16154)
                     /gene="dnaE"
                     /locus_tag="PXB27_001114"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDL5345915.1"
                     /translation="MSDCRFVHLKVHSDFSMINGLAKVKSLVKTAAANNMVAMGLTDF
                     INFCGLVKFYGEALGAGIKPIIGADVYVRTESGNEDYFELTLLAKNNKGYHNITQILS
                     KAYQQGYVEFPMVEREWLAELNEGIIILSGGRMGDVGKALLKENRQEAEEKLAFYQQY
                     YPNHFYLSVCRTGRADEERYIKQAVEFSQKNAIPLVAVNDVVFLKEDDFDAHEIRVAI
                     HDSYTLDDPKRPKKYSPKQYFRTEEEMCKLFADLPSALANTVEIAKRCSVTVRLGEYF
                     LPNFPTGELSTEDFLVKKSKEGLEERLEFLFPDPEERANKRSVYDERLQVELDVINQM
                     GFPGYFLIVMEFIQWSKDNDIPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFLN
                     PERVSMPDFDVDFCMDGRDRVIEHVAETYGRQAVSQIITFGTMAAKAVIRDVGRVLGH
                     PYSFVDRISKLIPPDPGMTLEKAFAAEPKLPELYEADEEVKDLIDMARKLEGVTRNAG
                     KHAGGVVIAPTAITDFSPLYCDSEGLHPVTHFDKNDVEYAGLVKFDFLGLRTLTIIKW
                     ALEMINERLAREGKEPIRIETIPLDDKKSFALLLAAKTTAVFQLESRGMKDLISRLKP
                     DCFEDIIALVALFRPGPLESGMVQNFIDRKHGKEEVSYPDPQYQHECLKPILEPTYGV
                     IVYQEQVMQIAQELAGYTLGGADLLRRAMGKKKPEEMAAQREVFEKGAEAKGIDGKLA
                     GQIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTSEMDNTDKIVG
                     FYDECMNMGLTVVPPDVNSGKHRFSVNEKGEIVYGLGAIKGVGEGPIEAILAARQKEG
                     KFKDLFDLTARVDLKKINRRTFESLIMSGAFDKLGPHRAALMKNLEDALKASDQHAKM
                     EELGQSDMFGVLTETPEEVQNAYANTPKWSEQVILDGERATLGLYLSGHPIGRFLKEL
                     SHYAPNRLNELQPTFRGQMTTVSGIVMASRVAVTKRGSRIGIATIEDRSGKLDITLFS
                     EALENFGHLMQQDQIIVATGSVQFDDFSGGLKMSAREVLSLDEARGRFAKSLALAISK
                     EQLSADFIKKMTAIITPHKEGTLPLHFYYQSPEGRVLLKGGVEWRISPNEAMFSELKA
                     LLGENSVELEFE"
     gene            complement(16379..>16965)
                     /locus_tag="PXB27_001115"
     CDS             complement(16379..>16965)
                     /locus_tag="PXB27_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HDL5345916.1"
                     /translation="KSLDNVLIYQFGEGQNKRELQSVLKARTANYSDEKGWVLSKVEK
                     STVGENQIQQAKQPDQVWQTSLTPSKLGIVSLKPESLSISGLADYVGFLKDTGQDPKR
                     FEITFWRKVFQPISMAVMMLLAISFIFGPLRSSTMGAKIVIGIVAGFMFYVANIVFGN
                     LSLIATWLPIPVGALIPSLLCLIIVWWLLSKKRD"
BASE COUNT         4785 a         3876 c         3367 g         4937 t
ORIGIN      
        1 aaagcaaaca taaaacatta ttaattttta acgacaaaat aactcttttg ggtaataatt
       61 tcatcagtta aaaaaattat caaatttaaa ttttatgagg aaccaaacta tggctaaaaa
      121 tacgcaacca tttgatgccc aagcagaagt gaatgagctt gttgaaaaag gcttaaaagc
      181 ccttgatgag tttagaaaac tcaaccaaga acaagtggat tttattgtcg ctaaagcctc
      241 tgttgctgcc cttgataaac acggtatttt agcaatgcac gcttacgaag aaaccggacg
      301 gggtgtgttt gaagacaaag ccactaaaaa cctgttcgcc tgtgagtatg ttgtcaataa
      361 tatgcgacat ttgaaaaccg caggggtcat tagtgaagat gatgtcaccg gtattaccga
      421 aattgccgac cctgtcggtg ttgtctgcgg tatcacccca accaccaacc caacctcaac
      481 caccattttc aaagcgttaa tcgcccttaa aacccgtaac cctatcgttt ttgctttcca
      541 cccgtctgct caacaatctt ctgcccacgc tgctcaaatt gtgcgtgatg ctgccgtggc
      601 tgccggtgca ccggaaaact gtattcaatg gattacccag ccttcaatgg aaggtacatc
      661 tgctttaatg aaacacccgg gcattgcaac tattttagcc accggcggta atgcaatggt
      721 tgaagccgct tactcttgcg gcaaaccggc attaggagtt ggtgcaggca acgtaccggc
      781 ttatgtagaa aaaaccgcta aattaaaaca agcggtgtat gacattgtaa tgtcaaaatc
      841 tttcgacaac ggtatgattt gtgcctctga acaagcggcg attgtagata aagaaattta
      901 cgctgagttt attaaagaga tgcaatctta cggcgtctat ttagtgaaca aaaaagagaa
      961 agccttattg gaaaaataca ttttcggtat tgataaagcc aaagatgaaa actgctcagg
     1021 tgcaaaactc aattcagcgg tggttggcaa accggctgcg tggattgcag aacaagccgg
     1081 ctttaaagta ccggaaaaaa ccaatattct gttagccgaa tgtgcctttg tgggcgaagc
     1141 tgagccactt acccgtgaaa aactttcgcc tgtactggct ctgttaaaat ccgactccac
     1201 cgaacacggt ttggaattgt ctgaagcgat ggtgaatttc cacggtttag ggcatagtgc
     1261 cgccattcat accgaaaaca aagccttagc caaaaccttt ggcgaacggg taaaagccat
     1321 tcgggtgatt tggaactcac catcaacctt tggcggtatc ggtgatgtat ataactcctt
     1381 cctgccatcg cttacgctgg gctgtggttc ctacggtaaa aactctgtag gtaacaacgt
     1441 tagtgcggtg aatttaatca atatcaaacg tgtgggcaga cggagaaata atatgcaatg
     1501 gtttaaagtg ccatcaaaaa tctactttga gcgtgactca attcaatatt tgaaatcaat
     1561 gcacgatgtg cataaagtaa tgatcgtaac cgaccgctca atggttgatc tcggctttgt
     1621 ggatagaatt accgaacaac ttcgccaacg tcgcaaccaa gtgatgattc agttctttac
     1681 cgatgttgaa ccaaacccaa gcctgcaaac cgtacaacgt ggtacagaat taatgcgtag
     1741 cttccagcct gatactatca tcgcacttgg tggtggttca ccaatggatg cagccaaaat
     1801 tatgtggtta ttctacgaac aaccggaagt cgatttccgt gatttagtgc aaaaatttat
     1861 ggatatccgc aaacgtgcat tcaaattccc acaattaggc agaaaagcga aatttgtcgg
     1921 cattccaacc acctccggaa caggctctga agtcacaccg tttgcagtaa ttaccgatgg
     1981 cgatattaaa tatccgctcg ctgactactc gcttacccca accattgcga ttgtcgatcc
     2041 tgcgttagta atgtctgtgc cgtctcacgt tgctgccgac accggtttag acgtattaac
     2101 tcacgctacc gaggcttaca catcgatttt agccaacgac tataccgacg gcttagcctt
     2161 gcaagccatt aagttagtgt ttgaaaactt ggaaaaatcg gtcaaagagt ttgatgaaac
     2221 cgcccgtgaa aaaatgcaca atgcttccac aatggcaggg atggcatttg ccaatgcctt
     2281 cttaggtatt tgccactcaa tggcacacaa aatcgggggt aaattccaca ccatccacgg
     2341 tcgaaccaat gcgattttac tgccacacgt tattcgctat aacggcacac gcccaaccaa
     2401 agtggcaact tggccaaaat acaccaatta tgtcgcagat gtgcgtttcc aagatattgc
     2461 cagaatgctt ggcttaccgg cagaaacccc ggaacaagcg gtggaagctt acgcccaagc
     2521 ggtacacgat ttagccgttc gttgcggcgt gaaaatgtcg cttcgtgagc aaggcgttga
     2581 tgagcaagcc ttcttgaatg cccgccgtga acttgcccta cacgcatttg aagaccaatg
     2641 cacaccagca aacccacgtt tggcaatgat tgaagatatg gaagcgatta tgactaaagc
     2701 ctactatggc aaggaataaa gcacaattta atcgttaaac ttactaaatg gccgcttgca
     2761 agcggtcatt tttcttgaaa tttttacttt tcccttcaca aaatactttc gctcttttac
     2821 aaagcatagt atttttatcg ctccttttat cttacaggaa atactatgca aattacgatt
     2881 ttagacggcg gaattagccg tgaattaatg cgtcgcaatg ctccatttca tcagcctgaa
     2941 tggtctgctg ccgctttgta tgaaggccca cattttgtgc aagcggtaca cgaagatttt
     3001 atcgctcacg gggcagaagt gattactacc gacagctatg cggttgtgcc gtttcacatt
     3061 ggcgaaaaac gctttgcggc agacggcaaa atgcttgccg atttagccgg tcgccttgcc
     3121 aaacaagcgg tcaaaaattc aggaaatatt gcaactcaaa ttgcaggctc acttccacca
     3181 atgtttggct cttaccgtgc cgatttaatt caaatggaac gctttgctga agtcgctcag
     3241 cctttaattg acgggctttc gccttatgtg gatatttggc tttgcgaaac ccaaagtgcg
     3301 attattgagc cgatttccat caaagcatta ttgccgaaag acaaccgccc gctttgggtc
     3361 tcttttaccc tcaccgatga tgaaccaacc accgagccac aacttcgctc gggcgaaagc
     3421 gttaaacaag cagtcgaaga aatggtgaag ttaggcgtgc aagcgatttt atttaactgc
     3481 tgtcagcctg aagtgatcgg cgaggcatta agtgtaaccc aaaaaacttt agccgaatta
     3541 aaagctaccc atattcgcac aggagcttat gccaacgcct ttgctccaca accaaaagat
     3601 gccaccgcca atgacggttt agatgaagtt cgccaagatt taaccctgga ggcttattta
     3661 ggctgggcaa aaaaatggac ggaacaaggg gcaactatta tcggtggctg ctgcggcatt
     3721 ggggttgagt atattgaaac gctggcaaaa aatttaaaat aaggaaatcg aatgtcaaat
     3781 aaaaaaatag gattaatttc actcaccgcc ttagtgctaa gctcaatgat tggctcgggg
     3841 attttcagct taccgcaaaa tatggcagcc gttgccggag cagaagccat taccattggc
     3901 tggctgatta ccggtgtcgg cattatcttt ttaggtttat cgtttttctt tatttcccgc
     3961 ttgcgtccgg atttagacgg tggcatttac acctacgcac gggagggttt tggcgagctg
     4021 atgggcttta tgtcagcgtg gggctattgg ctctgtgcca ccattgggat tgtaggatac
     4081 ctcaccgttg cttttgaagg tttgggtgta tttaccgaca acccgaacac cgtgattttc
     4141 gggcaaggta acaccctcgc ctcttttatt ggatcttcaa tcattgtgtg gctggttcac
     4201 gccctcatcg caggcggtat caaagaagct gcctttgtaa acttaattgc cacttttgtg
     4261 aaagtcgctc cgctcattct gtttatcggg ttaggcattt ggtatttcga tgtggatctg
     4321 tttaatagcg atgttaaagc caccgcactt aataatagtg taagcgacca agtgaaaagc
     4381 acaatgttaa tcaccttatg ggtatttacc ggtgtggaag gggcttctgt gctgtcggct
     4441 cacgcgaaaa agcgttctga cgtgggactg gcaaccgtat taggtattat tattgcactt
     4501 gctctttatg tggcaattac cattctttca ctcggaattc tgccacggga aactattgcc
     4561 aatatggcaa atccgtcaat ggggccgttg ctcgatgcaa tgatgggccc aaccggcaaa
     4621 gtgattatta ctgcttgttt aattgtatcg gtgcttgcct cttacattag ctggacgatg
     4681 tattcggcag aagtgcctta tcgtggctca aaaaacgggg ctttcccgaa aattttagac
     4741 aaagtgaata aaaacggcac gccgattaac tcactctggt ttaccggctt tatcgtgcaa
     4801 ttttgcttgg tgttggtgtt tgtttttgag caaagctata acaccttatt gctgatttcc
     4861 acctcgatga ttttaattcc ctatttctta atcggggctt atttattcaa attagcgatt
     4921 caaaccaacg ccacttggta cattaaactc accggcttta tggcagccct ttacggatta
     4981 tggattgtct atgccgcagg actagaatat ttgctgcttt cggtggtgct ttatgtaccc
     5041 ggaattttac tcttcctcta ctccaactat aaattcaacg gcagattaac cctttcaacc
     5101 gctgaaaagg tcatcttaat cgtgctactg gtaatttttg gctatgccgt gattgagctg
     5161 ccagcactat tagcagcata attcccatca aaaatgaccg cttgcaagcg gttatttttc
     5221 taggatcttc tgcaaagatt cattagaata gaaacaacgt caataataaa taaaaagagg
     5281 agaaacaatg caaacttatc aacataaagc cctttgtcgg gtaagaacca ttaccctttt
     5341 tctgagttta accaaagata aattgcagtg ggaaacggca ttaaatgcgg caaaagcaga
     5401 atttgatcta ttagttcccg ctattcaaac acaaggctat cacatacaat cgattcgaat
     5461 cgttaccaac agtttcgggg aatatcttga tctcactaat attgaaacag cgaaacagga
     5521 tttagcctat cttaaacagc tattaaccca acttaacgat agcggtttac gcattcgctt
     5581 tgccattggt gaggcaaaaa caccccacga attagcatta ctacctgagc tgattttaaa
     5641 ttatggtgat ttatctaacg cttgtgtcaa tattgaaccc gatgaatttg ggattttaga
     5701 caatgaaacc attttacaat ctgctcaaat tatcaaacaa atcgctgaaa ttaccccaag
     5761 aggtgaaggc aattttaatt ttacggtgaa ttttaactgt aaacctttta ttccttattt
     5821 tccggcaagc taccattcaa gcaagctccc taacagtttt gtgataggcc tagaaacacc
     5881 agatctatta gtagcggtgt taaaagagct gaaagccagc ccacataacg acttttatcg
     5941 ccaatgctat gaaacgatgt ctcaagcctt gcaatatcac gttgatgaag ttctcgaagc
     6001 agtaaaatcg gtacaacaca aattacaatt tgcctttgct ggcattgata gttctgctgc
     6061 tccgtcaaaa aactgtgcct caatgacaga agtttatgaa ttaatgggcg taccattttt
     6121 tggggcagcc ggaacagtag aagcctcttc acttctgaca aaagtcttta aatctatcaa
     6181 acatgtaccg cttgtcggtt tttcaggttt aatgttggca gtaaccgaag atttaggctt
     6241 agccgcaggc acacacaaag cccaattcga cattcgtgct ttactaactt atagtgctgt
     6301 ttgtggcatt gggttagata ccgtaccgat ttccaatgaa gccactgttg aacaaattgc
     6361 cgcattaatg cgagacaccg gcacaatggc atttagacta aataaaccgc ttacagtacg
     6421 tttatttcca attccgaata agagtgctgg ggaagtgaca gaatttgaaa gtgatgattt
     6481 atgtaactgc cgaattttag ctgtacctta aaataaaggc tgatggatat ttccatcagc
     6541 ctttcaattt acttcacctt caatttttta cctaccgtga tctgataatt tttcaaatta
     6601 ttcagctcac tgattttttg cggcgtagta ccgtaagctc tagcaatcga gtaaaccgtt
     6661 tcatcttgtt ggacaatatg gtagccactg ttagacacct taatttctct ttgagagtct
     6721 gattttttag actgattttt ttgatctttc tcaagagctg ttttttcttt tagttgctgc
     6781 tcttttttct tattggtttt agccgaatct tcttttttat cctctttttt gctcttttct
     6841 ttcaattttg tcttatctga ttgcgtattc ttctcttgtt tctcagaagc aggcttttta
     6901 gttaaatcag ctttagcagt tttttcctct ttcttatttt gcttatcttc tttgtccgat
     6961 tttttactga ccgaagaact agcaactgca ttttttgccc gataagcgac taatccatta
     7021 taaatggccc gagcaacttg tttacgataa tttgcactgg ctagctttgc ttcctcggtt
     7081 gaattagata aaaatcccgt ttccaccaat acagatggaa tatcaggtga acgcagaaca
     7141 cttaagcttg cgtgctgagg agacgatttt gctaatgcag ccacatttga cattttagct
     7201 aaaatgctct tgcctaattc atagccggca cgttgagagt gagagaactg taaatcaagc
     7261 actgtttgat ttaaataacg ttcattgttg cttgaaagca ccgaaccagc cccgccaagt
     7321 aattccgact gcttctcgtg gtcttccaac catttaccca tttcatcgct agcacggcta
     7381 ttagaaagta cccaaaccga agcccctttc aaggaatcgc cacgaggcga agagtctgcg
     7441 tgaatagaaa ccagcagatc ggcacgatgc ttacgggcaa tctcggtacg ttgcggtaat
     7501 tggataaaat aatctcccga gcgtgtcatc actgctttaa aattaggatc ggcatctaac
     7561 aaggctttta gctctttaga aattgcgagg gtaacgcttt tttccaaaat ccccagattt
     7621 ttaccaatag cccccggatc ttttcccccg tgtccggcat caatcgccac aatggttttg
     7681 gcaaatgtgg aaaaactgaa taaactcaat gtgctaaaaa caataaatct gataacttgc
     7741 ttcattacct tccctacaag cggtctattt ttgatgaaaa tttactaata tatctactgc
     7801 agtttgattt tgcggcaaca ggctaatctg ccgtccgtca ttcttataat caatttggat
     7861 cacaaaatca gcctctggaa tcattccttt acctttaact gcccattcaa gcaaacaaag
     7921 cgtttgtggt ttgaaataat cacgaattcc cataaactcc agctcttcag gatcggctaa
     7981 gcgatacaaa tcaaaatggt aaaggctgaa aggcggaagt tggtattctt ccaccagagt
     8041 ataagttggg ctttttacat tgccttgatg cccgaaagcc cgtacgatcg agcgggttaa
     8101 ggtggtttta cctgccccaa gttcgccgtt taggtaaaca accagcgaat gagcttggtc
     8161 ttgcaataaa tatttcttaa gttctaacgc gaatgattga ccaaattcca gcaatttagt
     8221 ttcatcttcg cagtaaaaag agagagaatc tgtcattatt tttggtctag ctgaataaaa
     8281 tgttcccgat aaaatttcaa ttcgttaatg gattcacgaa tatcgtccaa cgctaaatgc
     8341 gtattgcctt tactgaattt atctaaaatc tccggtttcc aacggcgtgc cagctctttt
     8401 aaagtactca catctaagtg gcgataatgg aaataatcgg ctaaatcagg catatattta
     8461 tacaaaaaac gtttatcttg agcaatgctg ttgccgcaaa tcggagaagc gccttttggc
     8521 acccagcgtt ttaagaagtc taaagtttgc aactctgctg cccgttcagt caatttgctc
     8581 gctttcactc gttcaatcag cccgttagcg gtatgggttt tgacgcacca atcactcatt
     8641 ttctctaata aatcatcggt ttggtgtacc gcaagtaccg gtccttccgc taaaatattt
     8701 aaatccttat cggtcacaat ggtggcgatt tcaataatgc gttctttttc cggatctaac
     8761 cctgtcattt caagatcgat ccaaattaaa ttttgttcgt tttgagccat tttatttcct
     8821 tacacttttg caatttttag ggcatttttg accgcttgcg gcacaaattg gctgatgtca
     8881 ccattgtggc gatagatttc ccttaccatc gttgaagaaa ggtaacgcca ttctattgaa
     8941 gggggaaaaa aaatagtttc taaatcgccc gataatttgt aatttaactg agcgagttgg
     9001 atttcatagt caatatcatc tgccccacga ataccacgga ttaacgcttt ggcattctgc
     9061 tgtttggcaa agtctgctaa caatccacta aagccaatca cctcaatatt ttgccactgg
     9121 gtacagcttt gtttaacaag ctccactcgt tcttcaagac taaaaagcgg ctgcttgctt
     9181 gggttttgag caacggcaac aatcactttt ccaaataagt gagaggcttt gcaaattatg
     9241 tccaaatgcc cattggtaat gggatcaaag gtgcctgcat aaattacgtt ataactcatt
     9301 ttatttctct aaataggagg ctaataaatc caaatggcgt tttaatgccc cttgattttc
     9361 ctgtaacacg cttaagccgg attttgcaat ttcttgtccg atttggggct gcttaagcaa
     9421 taattgaatg ctctcggtca aagcctcgac tgaactttca atttcaatca cgcctttcac
     9481 ctcacgcaat ttctcaaaga tttcagggaa attataggtg taaagcccag aaaccaccgg
     9541 aatattaaaa gcgattggtt caagtggatt atgcccaccg tgtttgacca aactgcctcc
     9601 tacaaaagca atgtcagaca acccatacag taacatcatt tcccccattg tatcacctaa
     9661 cattacttga gtattttggc ttaacgtttt attctcagta cgttttacat aatttaaatc
     9721 tgatttttga atcagtagct caactaaacc aaaacgctcc ggatggcgag gcactaaaat
     9781 caacactaaa ttcgggtaat gctctaataa gagtttatga gcatctaaaa tcagcttctc
     9841 ttccccttcg tgagtactgc cggcaatcca aatcggtctc tctgttaaat tcagactagc
     9901 tttagtttta atcaccgatt gatgaagctg tggcgtaatt tctaaatcaa atttcaggtt
     9961 accggtatta accatttttg ccggtgcata accgagtgct aaatagcgat ctcggcttac
    10021 ctcatcttga gccataatca gactaatttg gttgagcata tccttaatgc taccttttac
    10081 ccagccataa cgcttcgcag aacgtggcga taagcgggca ttggcaatca caaatggaat
    10141 tttacgaata tttactttac gaattaaatt cggccaaagt tcggtttcaa ttacaatcat
    10201 caatttcgga tcgataaaat tcaaaaaccg ctcaatagca tcaggtaagt cataaggcaa
    10261 ataaaaatgt gaaacactat taccaaaagc agcttgcaca cgcccagagc ctgttggcgt
    10321 taccgttgtg actatcaacg gaagttcagg atattgcttg ctgactgcct taatcaaaag
    10381 cgttgcagca atcacctcac caaccgatgc cgcatggatt accacccctt ttgctttcgg
    10441 tttttccgat tcgtcataaa tcccataacg ctcccataaa cgtttccgat aagccggttg
    10501 tttacgccct ttgtaccaca ttaatagcag aaccacaggc tgtaatagat aacttaaaca
    10561 aatatagagt aaacgtagca tttattttcc ttataccata accctcacct cttgtgggag
    10621 agagtcaaat acgccaataa atcgttcctc tcttgttcga gaggggggaa caagcggtta
    10681 tttttaccaa aaattttgca atctatatcc tcaaagacaa aaccacggaa taaccttaat
    10741 ccgtggttta tctcgcatca ttacgcttga tgtggattgc gggtattcgt attcactaag
    10801 ttacgcatta aaagtgcaaa atccaactca atatctgccg gcacatctaa aaagacaaaa
    10861 tgcccgtcac cgagtgctgc gtccacattt tcatttttgc ggttaagcat acgctcgatt
    10921 ttaaatacaa tgttgccttt aggggtcatc atttccactg aatcgcccac taagaatttg
    10981 tttttcactg ccacttccgc caagccttct gcattgcgtt tgccggtaaa ttcgcccaca
    11041 aattgttggc gttctgaaat cgaatagcca tattcatagt tttggtattc atcgtgagta
    11101 tgacgacgta agaagccctc tgtataacca cggtgtgcaa ggctttcaag agtatccatt
    11161 aaactttcgt caaacggttt accggcagcg gcatcatcaa tcgccttacg atacacctga
    11221 gccgttctcg cacagtaata gaacgatttc gtacgcccct caattttcag tgaatgcacc
    11281 cccatttgag ttaaacgctc aacgtgttgt actgcacgca gatctttaga gttcataaaa
    11341 taggtgccgt gttcatcttc aaaggcggtc atttgctcat cagggcgttg tgattctgta
    11401 taaaggaaca ctttatctgt tgcttcgcca ttacctagag taggagccac atttttgact
    11461 tcaatttccg gctcatactt aggcacaatt tgccctacct catcaatctt accttcttcc
    11521 attttatatt cccaacggca agcattggtg caagtgcctt ggtttgggtc acgcttattg
    11581 atatagcctg aaagcaagca gcgacctgaa tatgccatac acaaagcacc gtgaacgaag
    11641 atctcaagct ccatatccgg cacttgttca cgaatttctt cgatctcttc gagtgataat
    11701 tcacgggata aaatcacacg ggttagcccc atttgacgcc agaatttcac cgttgcccag
    11761 tttaccgcat tggcttgcac cgaaagatga atatccattt cagggaaatt ttcacgcacc
    11821 aacataatta agcccggatc agacataatc aaagcgtccg gcttcatatc cactaccact
    11881 tttagatctt tgatgaaggt ttttaatttt gagttgtgtg gcgcaatgtt taccaccaca
    11941 tagaattttt ttcctaaggc gtgggcttca tcaatcgcaa cttttaaatt ggcgtggtta
    12001 aattcattat tacgcacacg caagctataa cggggctgtc ctgcataaat cgcatcggca
    12061 ccataagcaa aagcgtaacg catatttttt aaagaacctg caggcgaaag tagctcaggt
    12121 ttggcatatt ttaaagtcat attattctct tttctgataa caagtcagga cttctcgacg
    12181 tgagaagcgt aagcggtata ttttctaaga aatattgcgt gatggcaagc taatccatta
    12241 caatccgatt tttatgtggg gaacaatatg aaacttaaat accttttagc aacaacatta
    12301 ctggcttttt caacatttac cgccgctcaa agcatcaaga cggagatgat gcaaatgaat
    12361 atgcaagcga ataaattgat gcgtgccgaa tcggcagaag agtttagcga aattgcgaat
    12421 gatttcatta taattaccga gaaaaccaaa gggatttttc ccaatagcgt agaaggcgat
    12481 aaagcagctc aggaagatta ccaagccggt atgcaaaaat taattgatat ggttaatcaa
    12541 gccagtgaga aagctcaagc aggtgaattg caagaagcaa aaatgctgat ttccgagctt
    12601 gaaatgatca agcgtgaata tcacaaaaaa tacaaataat tcaacacatt aaaagggtga
    12661 gctaaagctc accctttcta ttcaaattcc agttcaaccg aattctcacc aagaagtgcc
    12721 tttaattcgc taaacattgc ctcattcggg ctgattcgcc actctactcc gcctttgagc
    12781 aataccctac cttccgggct ttggtaataa aaatgcagcg gtaaagtgcc ttctttatgt
    12841 ggggtgatga tagcggtcat ttttttaata aaatctgcag aaagttgctc tttgctaatc
    12901 gctaacgcta aacttttggc aaatcgaccc cttgcttcat caagggataa cacttctcga
    12961 gccgacattt tcagtccacc gctgaaatca tcaaactgta ccgaacctgt tgccacaata
    13021 atttggtctt gttgcatcaa atgcccgaaa ttttctaagg cttccgagaa aagggtaatg
    13081 tccaatttcc ccgagcgatc ttcaatagtc gcaatcccaa tacggcttcc tcgcttggta
    13141 actgccactc gagatgccat aacaattcct gaaaccgtgg tcatttgccc tcggaaagtc
    13201 ggttgcaatt cattcagccg attaggggca tagtgggata attctttgag gaatctacca
    13261 atcgggtgtc cgcttaaata cagcccgagc gtggcacgct ctccatctaa aatcacttgc
    13321 tccgaccatt ttggcgtatt ggcgtaagca ttttgcactt cttccggtgt ctcggtaagc
    13381 acgccaaaca tatcgctctg cccgagttcc tccattttag cgtgctgatc tgaggcttta
    13441 agtgcatctt ccaaattctt catcaaggca gctcggtgag ggcctaattt gtcaaaagca
    13501 ccggacataa tcaggctttc aaaggtgcgg cggttgattt ttttcagatc gacccgagcg
    13561 gttaaatcaa atagatcttt aaatttcccc tctttttggc gagctgccag aattgcctca
    13621 atcggtcctt ccccaacgcc tttaattgct cctagcccgt aaacaatttc gcctttttca
    13681 tttacgctaa aacgatgttt accgctgttt acatcaggcg gcacaacggt taagcccata
    13741 ttcatacact catcgtagaa cccgacaatt ttatcggtat tatccatctc tgaggtcatt
    13801 accgcagcca taaattctgc cggataatgg gcttttagcc aaagtgtttg gtaagaaacc
    13861 aacgcataag ctgctgagtg agatttatta aagccgtagc cggcaaactt ttctaccaag
    13921 tcaaaaattt gaccggcaag tttaccgtca atccctttgg cttccgcccc tttttcaaac
    13981 acttcacgct gtgcagccat ttcctccggt ttcttcttac ccattgcacg gcggagtaag
    14041 tccgctccac cgagtgtata accggcaagc tcttgtgcaa tttgcataac ctgttcttgg
    14101 taaacaatga ccccataagt cggctcgaga ataggtttta agcattcgtg ttggtattgc
    14161 ggatcaggat aagaaacctc ttctttgccg tgtttacggt caataaagtt ctgcaccata
    14221 cctgattcca gcggccctgg gcggaatagt gccaccaatg cgataatatc ttcaaaacag
    14281 tccggcttca ggcgagaaat taagtctttc attccccgtg attcaagctg gaatactgct
    14341 gtagttttag ctgccagcaa cagagcaaag gattttttat catcaagcgg aatggtttca
    14401 atccgaatcg gctctttgcc ttcacgagcc aaacgttcgt taatcatctc caacgcccat
    14461 ttgatgatgg tgagcgtacg caagcctaag aagtcaaact tcaccaatcc tgcatattcc
    14521 acgtcatttt tatcaaaatg ggtaaccggg tgtaagccct cgctatcgca ataaagcggc
    14581 gaaaaatcgg taatcgcagt tggggcgatc accacaccac cagcgtgttt acctgcatta
    14641 cgagttacac cttcgagctt acgagccata tcaataaggt ctttcacctc ttcatcggct
    14701 tcatatagct caggcaattt tggctcagcg gcaaaggctt tttcaagcgt cattcctgga
    14761 tcgggcggaa tgagtttgga gatgcgatcc acaaagctat aggggtgtcc aagcactcgc
    14821 cctacatctc gaatgaccgc tttcgccgcc atagtaccga atgtgataat ttgcgatacc
    14881 gcttgtcgcc cgtaggtttc cgcaacgtgt tcaatcacac ggtctcgccc gtccatacag
    14941 aaatcaacgt caaaatcggg cattgaaaca cgctcagggt tgagaaaacg ctcaaaaagc
    15001 aaatcaaatt ccaatgggtc aagatcggta atcttcaacg cataagccac caatgagcct
    15061 gcccctgagc cacgacccgg tcctaccgga atatcattat ctttggacca ttggataaac
    15121 tccatcacga tcaaaaagta acccgggaac cccatttggt tgatcacatc taattcaact
    15181 tgcaatcttt cgtcataaac cgaccgcttg ttcgcccttt cctcaggatc agggaataga
    15241 aactctaaac gctcttctaa gccttctttc gattttttta ccaagaaatc ttcggtggaa
    15301 agctcaccgg tcgggaaatt tggcaggaaa tactcgccca aacgcacggt aaccgaacag
    15361 cgtttcgcaa tttcaaccgt gtttgccaat gcactcggta aatcggcaaa cagcttgcac
    15421 atttcctctt cagtacggaa atattgtttc ggtgagtatt ttttcgggcg tttcgggtcg
    15481 tctagggtgt agctgtcgtg aatggcaacc cgaatttcgt gtgcgtcaaa gtcatcttct
    15541 ttcaagaaga caacatcatt taccgccact aacggaattg catttttttg tgaaaattcg
    15601 accgcttgtt tgatatagcg ttcttcgtct gcccgtccgg tacggcaaac ggaaaggtaa
    15661 aaatgattcg ggtaatattg ctgataaaaa gctaattttt cttcagcttc ttgtcggttt
    15721 tctttgagta aggctttacc cacatcgccc attcgcccgc cggaaagaat gatgatgccc
    15781 tcattcagct ctgctaacca ttcacgttcc accatcggaa attccacata gccttgctga
    15841 taggctttgg acaaaatttg ggtaatatta tggtagcctt tgttgttctt cgccagtaat
    15901 gtcagctcaa aataatcttc attgccgctc tcggttctaa cataaacatc tgccccgata
    15961 atcggcttaa tccctgctcc caatgcttcg ccatagaatt tcaccaaccc gcaaaagttt
    16021 ataaaatccg ttaagcccat tgccaccata ttattagcgg cagcggtttt aactaaggat
    16081 tttaccttag ctaaaccatt aatcattgag aaatcactgt gaacttttaa atgaacgaaa
    16141 cgacaatctg acataggaat ataatctaag taaagaagtt tcccctcttt agtaaaggct
    16201 gtcaggttct aaaaagtgaa gccttctgtg aataatcccg ttgggcgaat gttattcgcc
    16261 cctacaaacg agcattattc ttgctcagag ttacatttaa aatccgaatg taggggcgaa
    16321 ttacattcgc cctttcagaa cctaacaacc ctgctttatc taaagaaggg agttaaaatt
    16381 aatcacgttt tttgcttaac agccaccaaa cgatgatcaa acagagtagg cttggaatta
    16441 atgctccaac cggtataggt aaccaagtgg caattaagct taaattgcca aacacaatat
    16501 ttgccacata gaacataaag ccggcaacaa taccaatcac aattttcgcc cccatcgtac
    16561 tactacgaag cggcccgaaa ataaaggaga tcgcaagtag catcatcacc gccattgaga
    16621 ttggttggaa tacttttcgc cagaaggtga tctcaaaacg tttcggatct tgtccggtat
    16681 cttttaagaa gccgacataa tctgccaagc ctgaaatcga gagcgactcc ggtttgagtg
    16741 aaacaatgcc taatttgcta ggggttaaac tggtttgcca aacttgatct ggctgtttag
    16801 cttgctggat ctgattttct cccacagtcg atttttccac tttagatagc acccagcctt
    16861 tttcatcact gtaattcgca gttcgggctt ttaaaacaga ttgcaattca cgtttgtttt
    16921 gtccttcgcc gaattgataa atcagtacgt tatctaagct ttttt
//
LOCUS       DANLUQ010000044        16855 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.044, whole genome shotgun sequence.
ACCESSION   DANLUQ010000044 DANLUQ010000000
VERSION     DANLUQ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16855
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.044"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..455)
                     /locus_tag="PXB27_001116"
     CDS             complement(<1..455)
                     /locus_tag="PXB27_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDL5345917.1"
                     /translation="MPFYAGQVKCIFIDPPYNTKSAFTHYDDNLEHSIWLSMMYPRLE
                     ILRELLAEDGSIWITLDDNESHYLKIMLDEIFGRKNFISNLIWEKKRKPSFLGKVGKV
                     TDNLFCYAKNKNELNELNDLTYLETSGDETYPLYNAGNSREILVFPPRS"
     gene            complement(866..3352)
                     /gene="torA"
                     /locus_tag="PXB27_001117"
     CDS             complement(866..3352)
                     /gene="torA"
                     /locus_tag="PXB27_001117"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HDL5345918.1"
                     /translation="MKKQDNIDVSRRGFLKNSSLSLAAGAGLASGTTGVVGALASTSA
                     KAAEMKTVVTAAHWGPLGVVVENGKVVKSDSVFEISVPNELQHVVADQVHGETRVKYP
                     MVRKGYLEGNKDTTLRGRDEWVRVSWDKAFELVANEMKRVREAHGANAIFGGSYGWYS
                     SGALHASRTLLQRYLNVTGGFVGVKGDYSTGAAQVIMPHVLGTIEVYEQQTSWEVILE
                     SSDIVVLWSANPLNTLRIAWTSTDQQGIEYFKKLKESGKRIICIDPVKSESCEFLGAE
                     WVPVNTGTDVALMLGMAHTLVAEGKHDQAFLKKYTTGFEKFEEYLLGKTDNQPKDAKW
                     ASQICGVPEETIKKLATDFQSKRTMLMAGWGIQRQRHGEQGHWMLVTLAAMLGQIGLP
                     GGGFGLSYHYSNGGVPSATGGILGSITANPAVEAGAKTWLDETSKSSFPVARISDALL
                     NPGKTIDYNGTQITYPDIKIVYWAGGNPFTHHQDTNTLVKAWQKPDTIIVNECHWTPT
                     ARMSDIVLPATTSYERNDLTMSGDYSMMNIFPMKQVVEPQFEAKNDYDIFAELAKRAG
                     KEAEFTEGKSEMDWLQSFYQTAFEAARKNRVIMPKFEKFWQENKPLSFKASDKAKKWV
                     RYEEFRNDPLLNPLGTPSGKIEIFSEVIAKMNYDDCKGHPTWMEHEEYSGKTTAEEPL
                     ALVTPHSKWRLHSQLAYSSLRKQYAVNDREPVLIHTEDAAARGITSGDIVRIFNKRGQ
                     VLAGAVVTDGIIKGTLALHEGAWYDPLDLGTSEQPLCKNGNPNVLTRDEGTSKLAQGN
                     SPNTATVQVEKFTGQAPEVTVFKEPKYTQG"
     regulatory      complement(3371..3567)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            complement(3571..4686)
                     /locus_tag="PXB27_001118"
     CDS             complement(3571..4686)
                     /locus_tag="PXB27_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HDL5345919.1"
                     /translation="MAKMKKIAVFVGIMALGAAVLGGTQYAMKATSSTEFCVSCHSMT
                     HPQAEWEGSVHFSNRKGIRAECSDCHLPQDGLHYVKAKIIALKDVWHTVVTNKLPDQE
                     AYEAHRLEMAQRVWAEMKENDSMTCKSCHSFDAMVLSEQTEAAQKMHKLAQETNQTCI
                     DCHKGIAHFMPDVPVDNAAAMGELAKHGAEFSTNDKTLYTLAMTPVQLAQGGEIRLLP
                     YAELHQWTAEGEQVKATVNGWQQEGAESVVYMDLGKRIMVALLPDDAQSKVNVTKTVF
                     DNVTNSNWKEINFEITAPKNAVTANITALNHFGDNLNQTHCSGCHAPIGAEHYTANQW
                     IGVVNSMKDRTSMSADDVRAVTIYLQRNAKDVAGSSH"
     gene            complement(4828..6579)
                     /locus_tag="PXB27_001119"
     CDS             complement(4828..6579)
                     /locus_tag="PXB27_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5345920.1"
                     /translation="MDKRSRWSCLVNEQGEISEWEDKAGADYFAELPQICHYAQALLY
                     SDKNSLQFYYSSNHQWLKISLERHQSFVLTQAEHTDLPYGLSKREIELLTLISSGLSN
                     EDIANELCISKRTVDKHIENIFAKTHTKSRTLLAVFAITEHLICLPTPGKLTQSILAS
                     FNIEQLALQIIAKKQPLVTASTRHEPPIFTINHHHSRPIIIGVPYVEQGIGQIDTREL
                     LNGTQLAVETINQQGGIHGREVQVATAGFYVKDKASILQAYHHLFDQEVDAISTAYAC
                     YLPEVHELIATKGIPYLHIATHSGSNKLAQNLPNQRIENMFQVCASDVTYGLGISHFL
                     QYYRYHYAPWLQNKTVMVVTVSWQKVDIGIESLTLSLREQGWNVDYLELTQEQDNYAT
                     LMAQVHNLAPSIIVFASYFAEDIVQFYQTFIRNPTNAIIYSIYAPSAFLPHEKLCEGV
                     LWSTNSGLSENYLGRQFSQHYERFFGKSPTYSQASIAYDQANILANAWKQSVSPRHFK
                     AVSNAIRLQPHYGVNGTYYFNTDSQIGLTYAETHDLSISLPQLVYQIQQGQSRVIAPE
                     LFANAQFILPPWFSEKA"
     regulatory      6743..6899
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            6888..7913
                     /gene="moaA"
                     /locus_tag="PXB27_001120"
     CDS             6888..7913
                     /gene="moaA"
                     /locus_tag="PXB27_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDL5345921.1"
                     /translation="MQSIIPIKNVGDDVVSGLVDRFQRQYTYLRLSITDVCNFRCNYC
                     LPDGYKPPSHKQTFLSVDEIQRVAKAFANLGTEKIRITGGEPTLRKDFLEIAHTISQT
                     PGIRKVALTTNGYRLERDVELWQQAGITDINVSVDSLDPRQFQLITGENKLQSILKGI
                     DKAFEIGYPKIKVNAVLMKQYTAPELDKFLTWIKDKPIQMRFIELMETGEMDSFFQAQ
                     HLSGQSVMTRLLQEGWQLQPKAMSDGPAKVLSHPDFKGEIGLIMPYEKNFCASCNRLR
                     VSALGKLHLCLFGEEGIDIRDLLASDEQQLQLETRLKSSLQGKREHHYLHIGDSGVRN
                     NLASIGG"
     gene            8414..8890
                     /gene="moaC"
                     /locus_tag="PXB27_001121"
     CDS             8414..8890
                     /gene="moaC"
                     /locus_tag="PXB27_001121"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HDL5345922.1"
                     /translation="MTTFTHINQNGEANMVDVSMKQETVRIARAEAFVRMNAETLQMI
                     VSGNHHKGDVFATARIAGIQAAKRTWELIPLCHPLLLSKVEVQLEALPESNQVRIESL
                     CKLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMIIENVRLLSKSGGKSGDFKVD"
     gene            8883..9128
                     /gene="moaD"
                     /locus_tag="PXB27_001122"
     CDS             8883..9128
                     /gene="moaD"
                     /locus_tag="PXB27_001122"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005631889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HDL5345923.1"
                     /translation="MIKILFFAQVRELVATDRLDVEPTFTTAEALREHLSQQGKKWQL
                     ALEKGKLLVAINQTICPLETEIKDGDEVAFFPPVTGG"
     gene            9130..9585
                     /gene="moaE"
                     /locus_tag="PXB27_001123"
     CDS             9130..9585
                     /gene="moaE"
                     /locus_tag="PXB27_001123"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HDL5345924.1"
                     /translation="MFETLIEVQEAEFDQNRIYQWLSEQHSVGAATLFIGKVREMNLG
                     DNVSGLFLEHYPAMTKKALQEIVAQARTRWDLQRVAVIHRIGQLHTGDEIVLVGVSSA
                     HRGDAYHANEFIMDYLKTKAPFWKREQTQAGERWIEGRESDQQAAKKWQ"
     gene            9618..10100
                     /gene="ybeY"
                     /locus_tag="PXB27_001124"
     CDS             9618..10100
                     /gene="ybeY"
                     /locus_tag="PXB27_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDL5345925.1"
                     /translation="MENLYIDLQIACENTENLPSEQQFYTWVQKALAVEAKTDDFPES
                     EITIRIVDEAESHELNLTYRGKDKPTNVLSFPFEAPEGIEMPLLGDLVICRQVMEKEA
                     EEQGISAESHWAHLAIHGTLHLLGYDHIEEDEAVEMESLETEIMQSLGYEDPYLSEKI
                     "
     gene            10178..12196
                     /locus_tag="PXB27_001125"
     CDS             10178..12196
                     /locus_tag="PXB27_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Met) cytidine acetyltransferase"
                     /protein_id="HDL5345926.1"
                     /translation="MRTIAIITSPNNADFIQQGYFYIPENHQLKAIPFSNAKTILGQE
                     YPFAIYTMQAENGVNFNLDAFAILAGTIQENGTLFLLCPQWDNLENELDFDALRWNEN
                     QAIACPNFYQHFKQLVAKFGFEVRTNLPKLPATSGQIPSKIYQLTQEQQNILQNLPLD
                     PADIHLITAPRGRGKSTLAGRLAKALPAQNKVLITARSYSALPSFWRSAEQKIPFFAP
                     DNLIEQVKSKNISPNQWLFIDEAASLPLPILHTLCAYFEKVVLTTTTQNYEGTGRGFE
                     LKFPVMLDKPFKHWTLSQPLRWSENDPLEAFVTELLLLSEEKSPLFSKEGKGEIYSLR
                     SHYVPLLKQRSNAKRLWQKQLSKNISYSNKHLQEKIAFYHLLANAHYKTTPTDLRRLF
                     DGENQLLFSQQENEQLIGGIWAMQEGGLDEKLTQAIWLGERRPQGSLVAQYLCFQGNL
                     PQACKLHSIRISRIAVLPEKQNQGVGKRLISQFILQNSVEKRPLDFISVSFGLTTPLH
                     AFWQSCGFQLVQITPNKEASSGYQSAMMIYPISEQGKLFAQQATQAFARDFALQPFFT
                     EICEELQNFEQFRPLATHNLDERDWKNLTAFAHGKRTFSAAYVSLKRLYLSDKARFSE
                     LDIFERKGQKISKMEVENYRKLVLQNLNNKSFKIIYRFYYIFLCFCSC"
     gene            12261..13451
                     /locus_tag="PXB27_001126"
     CDS             12261..13451
                     /locus_tag="PXB27_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HDL5345927.1"
                     /translation="MFSHIQAAPADSILGLGEAFKADSREGKINLGIGVYMTDEGKTP
                     IVKAVKEAEKRLLETENSKNYLTIDGVQAFNAATQALLFGEGAEVITSGRAKTAQSLG
                     GTGALRIAAEFIKRHTNAKNVWISTPTWPNHNGIFDAVGVNVKGYRYYNKETNGLDWE
                     NLIADLSQAEAGDVVLLHGCCHNPTGIDPTPAQWEQLAALSAEKGWLPLFDFAYQGFA
                     NGLEEDAYGLRAFVKNNRELLVASSFSKNFGLYNERVGAFTLIADNADDANRAFTQIK
                     SIIRVLYSNPSAHGASAVAVALADPDLKAQWIAELDEMRNRIKEMRSQLVQKLKEKGA
                     NKDFSFITEQNGMFSFSGLTPEQVDKLKDEFAIYAVRSGRINVAGITSKNIDALADAI
                     VKVL"
     gene            complement(13558..14859)
                     /gene="folC"
                     /locus_tag="PXB27_001127"
     CDS             complement(13558..14859)
                     /gene="folC"
                     /locus_tag="PXB27_001127"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDL5345928.1"
                     /translation="MNTLSIPKATDSLETWLSYLEQAHSKPIDMGLDRIRKVATELDL
                     LKPAPYVITVAGTNGKGSTCRLLEVTLLKAGFRVGVYSSPHLIHYNERVRINGEVLPD
                     ADHVQAFAKINAKKTASLTYFEFSTLSAFELFKQNQLDIVILEVGLGGRLDATNIIDP
                     SLAVIISIDIDHVAFLGDNREDIGREKAGIFRENIPVVIGEPDCPHSIKQIADSLNCH
                     AFYRGKDWQFEIKNNLFHWYSEEKSFTDLPIPLIPIPNAGTALAVLMQLPFEIDEKII
                     RQALNEAQMTGRFQTLTDTDFTHFSQPKPAAKVIIDVGHNPHAARYLADKLRELKQPN
                     QKIYAVFSALEDKDLSGIVEPLANIIDEWHCAGLDCWRGQTGEAVLAKLVNVLPNSTA
                     CSYENVAQASRVLFETANENDIVLVFGSFHTVADFMLWLET"
     gene            complement(14869..15765)
                     /gene="accD"
                     /locus_tag="PXB27_001128"
     CDS             complement(14869..15765)
                     /gene="accD"
                     /locus_tag="PXB27_001128"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HDL5345929.1"
                     /translation="MSWLDRILGRDSSSSSNAKSKVPEGVWTKCTSCEQVLYSEELQR
                     NQQVCPKCDHHMRIDARTRLLNLLDKDSAVEIAADLEPEDVLKFKDLKKYKDRLTAAQ
                     KQTGEKDSFIVLAGTLHNMPVVTASFNFEFMGGSMGSVVGAKFVKAAEKALAEKIPFI
                     CFSASGGARMQEALFSLMQMAKTSAILAKMKEQGVPFISVLTDPTLGGVSASLAMLGD
                     VNIAEPKALIGFAGPRVIEQTVREKLPEGFQRAEFLLEHGAVDMIVHRKEMRDTLSRL
                     CAKLTNQPTPFKVAELVVENAE"
     gene            16074..16855
                     /gene="mazG"
                     /locus_tag="PXB27_001129"
                     /pseudo
     CDS             16074..16855
                     /gene="mazG"
                     /locus_tag="PXB27_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600778.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
BASE COUNT         4923 a         3524 c         3496 g         4912 t
ORIGIN      
        1 atagaacgag gagggaaaac taaaatttct cttgaatttc cagcattgta taatggatag
       61 gtttcatcac ctgaggtctc aaggtaagtt aagtcattta attcatttaa ttcatttttg
      121 ttttttgcat agcaaaataa attatctgta actttaccaa ctttgcctaa aaaagatggt
      181 ttccttttct tttcccaaat taagttagaa atgaaattct tccgcccaaa aatttcatca
      241 agcataattt tcaaatagtg actttcgtta tcatcaagag tgatccaaat tgaaccatct
      301 tcggcaagta actctcgcag aatttcaagg cgtggataca tcattgaaag ccaaatggaa
      361 tgctctaaat tatcatcgta gtgggtaaag gccgattttg tgttataggg aggatcaata
      421 aaaatacatt tgacttgccc cgcataaaag ggcaaaagag attttaaggc tgataaatta
      481 tcaccctgaa taattaaatt ttgcttgact ttagataagg tttcagcaga agctgagctg
      541 tcggctgtcg gctgtcggct gtcggctgtc ggctgtcggc tgtcggctgt cggctgtcgg
      601 ctgtcggctg tcggctgtcg ggcaatatta tgcgagaagt ccggcaactc acgcaatagg
      661 caatatgggg tttgttttgc ttttttgacg gcttcttctt tgccgtacca agtaagggtt
      721 gtcataaaat gcacctgaaa agtaaaagtg gattattgta acaagcggtc aaaaaaatag
      781 aaagttttac aaaaaattaa gcaaatttaa ccgcttgcaa catagaaaag gcacagtttt
      841 atactgtgcc tttggttata tgaaattagc cttgtgtgta tttaggctct ttaaatactg
      901 ttacttctgg tgcttgtccg gtgaattttt caacctgtac cgtagcggta tttggtgagt
      961 taccctgtgc taattttgat gtgccttcat cacgggttag cacgttaggg ttgccgtttt
     1021 tacatagcgg ttgctcgctg gtgccgagat cgagcggatc gtaccaagct ccttcgtgca
     1081 aggctaatgt acctttgata atgccatctg tcaccaccgc accggctaat acttgaccac
     1141 gtttgttaaa aatacgtaca atatcaccgc ttgtaatgcc acgagcggcg gcatcttcag
     1201 tatggatcaa caccggctca cggtcattaa ccgcatattg tttgcgtaat gatgagtaag
     1261 ctaactggct gtgtaaacgc catttagagt gtggagtcac caatgccaac ggttcttctg
     1321 cggtggtttt acccgagtat tcttcgtgtt ccatccaagt tgggtgacct ttacagtcat
     1381 cataattcat tttggcaatc acctcagaga aaatctcaat tttgccggac ggtgtgccta
     1441 atggatttag gagtgggtca ttacgaaatt cttcataacg aacccatttt ttcgctttgt
     1501 ctgatgcttt aaaagaaagt ggtttattct cttgccagaa tttctcgaat ttcggcataa
     1561 tcacacgatt tttgcgtgct gcctcaaatg cagtttgata gaaactttgt agccaatcca
     1621 tttcagattt gccttcggta aattccgctt ctttgcctgc acgtttggct agttcggcaa
     1681 aaatatcgta gtcgtttttt gcctcaaatt gcggttcgac cacctgtttc atcgggaaaa
     1741 tattcatcat tgaataatcg cccgacatag ttaagtcatt acgctcatag ctggtggttg
     1801 ccggcagcac gatgtcagac atacgagccg ttggtgtcca atggcattca ttgacgataa
     1861 tagtatccgg tttttgccac gctttcacca aagtgttggt gtcttggtgg tgggtaaacg
     1921 gattaccccc tgcccaatag acaattttga tatcaggata agtaatttgt gtgccattgt
     1981 aatcaatggt tttgcccggg ttgagcagtg catcggaaat acgagccacc gggaatgaag
     2041 attttgatgt ttcatctaac caggttttcg ctccggcttc tactgccgga tttgctgtga
     2101 tagagcctaa aatgccccct gtggcactcg gcacgccgcc gtttgagtaa tggtagctta
     2161 aaccgaaacc accacccggt aagccgattt gacctaacat tgcagctagg gtgactaaca
     2221 tccaatgccc ttgttcaccg tgtcgttggc gttgaatccc ccagcctgcc attaacattg
     2281 tgcgtttaga ttggaaatca gtcgctaatt ttttaatggt ttcttccggc acaccacaaa
     2341 tttgacttgc ccattttgca tctttcggtt gattgtctgt tttaccgagc agatattctt
     2401 caaacttctc aaaaccggtg gtgtattttt taaggaatgc ttgatcgtgt ttgccttcag
     2461 cgactaaggt atgagccata ccgagcatta acgcaacatc agtgccggtg tttaccggta
     2521 cccattctgc acctaaaaat tcgcaagatt cactttttac cgggtcaata cagataatgc
     2581 gtttcccgct ctctttgagt tttttgaagt attcaatacc ttgttgatcg gtagaagtcc
     2641 acgcaatgcg tagcgtattt aacgggttag ctgaccaaag caccacaata tctgaacttt
     2701 ctaaaatcac ttcccaacta gtttgctgtt cgtacacctc gattgtaccc aagacgtgtg
     2761 gcataatcac ttgagcagca ccggtagaat agtcgccttt tacaccaaca aatccgccgg
     2821 taacatttaa atagcgttgt aacagtgtac gggaagcgtg taatgcccca gaactatacc
     2881 aaccgtaaga accaccaaaa atagcattcg ccccgtgagc ctcacgaaca cgtttcattt
     2941 cattcgcaac taattcaaat gccttatccc acgaaactcg cacccattca tcacgcccac
     3001 gtagagtagt atctttatta ccttctaagt agcctttacg caccattggg tattttactc
     3061 gtgtttcacc gtgtacttgg tctgctacca cgtgttgcaa ttcatttggc acagaaatct
     3121 caaaaacaga atcagatttc accactttgc cattttctac cactacacct aaaggccccc
     3181 agtgggcggc agttactact gttttcatct cggctgcttt ggcactggtg gaagctaatg
     3241 cccccactac accggtagta ccactggcta aacctgcacc ggcagctaag cttaatgatg
     3301 aattttttag aaaaccgcga cgacttacgt caatattgtc ttgttttttc ataataaacc
     3361 tcgtttgaag ttaagttaaa aatagacacc tttctaaagt cgagtggata aaccatttct
     3421 cgtgtatcca ttggtaaaag caccgacggt aatttttgca aaatgttagg aaaaaatgac
     3481 cgcttgtagt aggtgcttta cggcttgcta ccatatctct gctctcagca gacacttagg
     3541 ctcacaagct cggtatcctc taagttgcaa ttaatgggaa ctgcctgcta catcttttgc
     3601 attacgttgg agatagatag tcaccgcacg cacatcatca gccgacatag atgttctgtc
     3661 tttcattgaa tttaccaccc caatccattg atttgcggtg taatgttctg ccccaatcgg
     3721 tgcatggcaa ccgctacagt gtgtttggtt taagttatcg ccaaaatgat ttagtgcagt
     3781 aatattggca gtgactgcat ttttaggagc agtaatttca aaattgattt ctttccaatt
     3841 tgagttggtg acattatcaa acactgtttt tgtgacattc actttgcttt gagcgtcatc
     3901 aggtagaaga gcaaccataa tacgcttgcc taaatccata tacaccacag attccgcacc
     3961 ttcttgttgc cagccattaa ccgttgcttt aacctgctcg ccttctgctg tccattgatg
     4021 taattctgca taaggtaaca agcgaatttc tccaccttga gcgagctgca ctggagtcat
     4081 tgcgagcgta taaagggttt tatcatttgt gctaaattcc gcaccgtgtt tagctaattc
     4141 gcccattgct gcggcattat ctaccggcac atcaggcata aaatgggcaa ttcctttatg
     4201 gcaatcaata caggtttgat tagtttcttg ggctagtttg tgcattttct gtgccgcttc
     4261 tgtttgttca gaaagtacca tcgcatcaaa actatgacag cttttacagg tcatagaatc
     4321 gttttctttc atttctgccc aaacacgttg tgccatttct agacgatggg cttcataggc
     4381 ttcttgatca ggtaatttat tggttacaac ggtgtgccaa acatctttta atgcgatgat
     4441 tttagctttg acataatgca agccgtcttg tggtagatgg caatccgaac actcggctcg
     4501 aattccctta cggttggaaa aatggacact gccttcccac tctgcctgtg ggtgagtcat
     4561 agaatggcag ctcacacaaa attctgttga gctggtggct ttcattgcat attgtgtgcc
     4621 acctaatacc gcagcaccta atgccataat accgacaaat acagcaattt ttttcatctt
     4681 agccataaat taccccactt aatacactaa aaattaaaga cagaggcatt gtattaactc
     4741 ccaagaggta taaaaataag taaaaatacc tattacctcg aaagagtgag aataatttga
     4801 tctaaggcaa aaatatgtag tgaaatttta ggctttttca gagaaccacg gcggtaagat
     4861 aaattgtgca ttggcaaaca actccggtgc aatcacacgg ctctgccctt gttggatttg
     4921 gtaaaccaac tgtggcaagc tgatagataa gtcgtgggtt tctgcgtaag ttaatccaat
     4981 ttggctatcg gtattaaaat agtaagtgcc gtttacccca taatgtggct gtaagcggat
     5041 cgcattggag acggctttaa aatgccgtgg tgaaacactc tgtttccacg catttgccag
     5101 aatattcgct tggtcataag caatactggc ttgggaataa gtgggcgatt tgccgaaaaa
     5161 ccgttcgtaa tgttggctaa actgtctgcc taaataattt tcgcttaacc cactattggt
     5221 actccaaaga acgccttcgc aaagtttctc gtgtggcaaa aacgccgaag gtgcataaat
     5281 actgtaaata atggcatttg ttgggttgcg aatgaacgtt tgataaaact gtacaatatc
     5341 ttctgcaaaa taagaggcaa acacaataat ggaaggtgcg agattatgca cctgtgccat
     5401 taatgtggca taattatctt gctcttgggt gagttccaga taatccacat tccagccttg
     5461 ctcccgcaaa cttagggtta aactttcaat accgatatca actttttgcc aactcaccgt
     5521 taccaccatc accgttttat tctgaagcca aggggcataa tggtaacggt aatattgcag
     5581 aaagtgagat atacctaagc cataggttac atcactggcg caaacctgaa acatattttc
     5641 aattcgttga ttcggtaaat tttgggcaag tttatttgag ccactatggg ttgcaatatg
     5701 caaataagga atgcctttag ttgcaattaa ttcgtgtact tccggcaagt agcaggcata
     5761 agcggtggaa atcgcatcaa cttcttgatc aaataaatga tggtatgcct gaagaatact
     5821 tgctttatct ttaacataaa acccagccgt tgcaacctgt acttctctgc cgtgaatacc
     5881 accttgttga ttgatggtct caaccgcaag ttgagtgccg ttaagtagct ctcgggtatc
     5941 gatttgccca ataccctgtt caacataagg cacgccaata ataatcggac gactatgatg
     6001 gtggtttatc gtaaaaatcg gtggttcgtg tcgagttgaa gccgttacaa gcggttgttt
     6061 tttcgcgata atttgcaatg ccagttgttc aatgttaaag ctggcaagaa tggattgggt
     6121 tagttttccc ggtgtgggta aacagattaa atgttcggta atggcaaaaa cagctaataa
     6181 ggttcggctt ttggtatgtg ttttcgcaaa aatattttcg atatgtttat caacagtccg
     6241 tttgctgata caaagctcat tggcgatatc ctcattgctc aaaccactgc taattagagt
     6301 gagaagctca atctcccttt tactcagtcc ataaggcaaa tcggtatgct ctgcttgagt
     6361 caatacaaac gattggtggc gttctaggct gatcttaagc cattgatggt ttgaactata
     6421 gtaaaactgc aagctatttt tatctgaata gagcaatgcc tgtgcatagt ggcaaatttg
     6481 cggtaattca gcaaaatagt ctgcccctgc tttgtcttcc cattctgata tttcgccttg
     6541 ttcatttact aaacaagacc atcggctacg tttatccatt ctcacctcga actaaaaacg
     6601 ttagctaaaa tagcaaaatt atctcggcat taatatgata taaatcaaac taagcaacgg
     6661 ttgccagatt tttataggta gagcatacaa tttaaattat acctcttgcc taattaggtt
     6721 tctcaccata aaatgaaaac caatttattg aagactccga gcattatttc ctaactctaa
     6781 aaattaaaga caaatttttg atatggaatt tatgccagta acagcataaa gctgttcaaa
     6841 ggataactta gaaatgggtt gtcctctcgt gtttagaaag gtgtcaaatg cagtctatta
     6901 tcccaattaa aaacgtgggt gatgatgtcg tttcaggttt agttgatcgc tttcagcgtc
     6961 aatatactta tttgcgacta tccattactg atgtttgtaa cttccgttgt aattattgct
     7021 tgcctgatgg ctataagccg ccttcacata agcaaacttt tcttagcgtt gatgaaatcc
     7081 agcgggtggc taaagccttt gccaatttag ggacggaaaa aatccgtatc accggtggtg
     7141 agccaacttt acgcaaagat ttcctcgaaa tcgctcacac tatttcacaa acacccggta
     7201 tccgtaaagt tgccctaacc accaacggct atcgcctaga gcgtgatgtt gaactgtggc
     7261 aacaagccgg tattaccgat attaatgtga gtgttgatag ccttgaccca cgccaatttc
     7321 aactgattac cggcgagaac aagttgcaat ctattttaaa gggtattgat aaagcctttg
     7381 aaattggcta cccaaaaatt aaagtcaatg cggtgctaat gaagcaatat accgcccctg
     7441 aattggataa attcctcact tggatcaaag acaagccgat tcaaatgcgg tttattgagc
     7501 tgatggaaac cggtgaaatg gatagtttct tccaagctca acatctttct ggtcaatcgg
     7561 tgatgacccg cttattacaa gaaggctggc aattacaacc caaagcgatg tctgacggcc
     7621 cggcaaaagt gctttctcac cctgatttta aaggcgaaat cggcttaatt atgccctacg
     7681 agaaaaattt ctgtgcctct tgcaaccgct tgcgtgtttc cgcacttggt aaactgcatc
     7741 tttgcttatt tggtgaagaa gggattgata ttcgtgattt actggcaagc gatgagcaac
     7801 agttacagct tgaaacccgc ctaaaatctt ccttacaagg caagcgagaa caccactatt
     7861 tgcatattgg ggatagtggg gttaggaata atttggcgag tatcggtggt taaacaacca
     7921 ccgagcgtgt tttttctgtt acttttcttt gcttcgccaa agaaaagtaa ccaaaagaaa
     7981 ggcgacccca gattttcctt aatccttcgc ttgttgctaa cttttgggac gaacttttca
     8041 aactcgccaa ctgcgttggc tcaaacacga aaagttgtcc tcaaaagttt acgcaatcgc
     8101 tacggcggaa aataggggca agcggtacat tatttaagat ttttgcaagt ttttatttaa
     8161 aaataaccgc ttgtaatgtt ccagcttctc ccttctgaac cgcctgagcg actttgaatt
     8221 tataggaaaa atttagtgtt tgagccaacg aagttggcga gttttaaatt tttccgttaa
     8281 acaaattcaa ataaaatgaa gatagcggtg aagtagggtg tgctttcttt ggttactttc
     8341 tttgcacaag caaagaaagt aacaagaaga cgctcaatat taattttaca atcaaattta
     8401 aagagacaaa caaatgacaa ccttcaccca catcaaccaa aacggcgaag ccaatatggt
     8461 tgatgtttca atgaaacaag aaaccgtacg cattgcccga gcggaggcat ttgtgcgaat
     8521 gaatgccgaa acattacaaa tgatcgtcag tggtaatcac cacaaaggcg atgtattcgc
     8581 aaccgctcga attgccggta ttcaggcggc gaagagaact tgggagctga ttccactctg
     8641 ccacccgtta ttgctttcta aagtggaagt tcaactggag gcgttgccgg aaagcaacca
     8701 agtgcgaatt gaatcactct gtaaactaac cggcaaaacc ggcgtggaaa tggaggcatt
     8761 aaccgcagct agtgttgctg ctctgactat ttacgatatg tgtaaagccg tgcaaaaaga
     8821 tatgattatt gaaaatgtaa gattacttag taaaagtggc ggaaaatcag gagatttcaa
     8881 ggttgattaa gatacttttt ttcgcccaag tgcgtgaact tgtggcaacg gatagactag
     8941 atgttgagcc tacttttacc actgccgaag cgttgcgtga acacttaagc caacagggta
     9001 aaaaatggca acttgccctt gaaaaaggca aactgctggt tgcaattaat caaacgatct
     9061 gcccgcttga aacggagatc aaagacggcg atgaagtggc attcttccca ccggtaaccg
     9121 ggggctaaaa tgtttgaaac cctgattgaa gtacaagaag ctgaatttga ccaaaaccgc
     9181 atttaccaat ggctcagtga acaacatagc gtgggggcag ccactctatt catcggtaaa
     9241 gtacgtgaaa tgaatttggg cgataatgtt tccggtttgt ttcttgaaca ctatccggca
     9301 atgactaaaa aagcattgca agaaattgtg gctcaagctc gcactcgttg ggatttacaa
     9361 cgcgttgcgg tgattcaccg cattgggcaa ctgcataccg gtgatgaaat cgtattagtc
     9421 ggtgtgagtt ctgctcatcg aggtgatgcc taccacgcca acgagtttat tatggattat
     9481 ctcaaaacca aagccccatt ttggaaacga gaacaaaccc aagccggtga acgttggatt
     9541 gaagggcgtg agagtgacca acaagccgcc aaaaaatggc aatagaaaaa atggcaataa
     9601 tttgcaaaag gtaacgaatg gaaaatttat atattgattt gcagatcgcc tgcgaaaata
     9661 ccgaaaactt accaagtgaa caacaatttt atacttgggt acaaaaagcc ctcgcagttg
     9721 aggcaaaaac cgatgatttc cctgagagtg agatcaccat tcggattgtc gatgaagcag
     9781 aaagccacga gctaaatctg acttatcgtg gtaaagacaa gccaaccaat gtgctatctt
     9841 tcccgtttga ggcccccgaa ggaatcgaaa tgccactatt gggtgattta gtcatctgtc
     9901 gtcaagtaat ggaaaaagag gcggaagaac aaggtatttc agccgaatcc cattgggctc
     9961 atttggcgat tcacggcaca cttcatctgc tcggctacga ccatattgag gaagatgaag
    10021 cagtggaaat ggaaagcctc gaaaccgaaa ttatgcaaag tttagggtat gaagatccgt
    10081 atctttcaga aaaaatatag tcttgtaggg gagggtcctg tgccctccag tactccatta
    10141 taatcggaca ggcacaggag ctgttcctac aagttatatg agaacgatcg caatcataac
    10201 atctcctaac aatgccgact ttattcaaca aggctatttt tacattcccg aaaatcatca
    10261 gcttaaagct atcccctttt ccaatgccaa aacgatttta ggacaagaat acccttttgc
    10321 catttatact atgcaagcag aaaatggggt gaattttaac ttagatgctt ttgcaatact
    10381 tgccggcact attcaagaaa acggtacttt atttttgctt tgtcctcagt gggataattt
    10441 ggaaaatgaa ttagatttcg atgcgttacg ttggaacgaa aatcaggcta ttgcctgccc
    10501 gaatttctat caacatttta aacaattagt cgctaaattt ggttttgaag tcagaactaa
    10561 tttgccaaaa ttaccggcta caagcggtca aattccttca aaaatttacc aattaacgca
    10621 agaacagcaa aatattttgc aaaatctacc gcttgatcct gccgatattc atttgattac
    10681 cgcccctcgt gggcggggca aatctaccct tgcagggcga cttgcaaaag cgttacccgc
    10741 acaaaataag gtattaatta ccgcacggtc ttattctgca ttgcccagtt tttggcgttc
    10801 ggcagagcaa aaaatccctt ttttcgcacc tgacaattta atcgagcaag ttaaaagtaa
    10861 aaacatttca cctaaccaat ggctctttat tgatgaggca gctagcctac ccttgccgat
    10921 actccacaca ctttgtgcct attttgaaaa agtggtttta accacaacaa cccaaaatta
    10981 cgaaggcacg gggcgaggat ttgagctgaa attcccggta atgttggata aaccgtttaa
    11041 acattggacg ttatctcaac cattgcggtg gagcgaaaat gatcctctcg aagcatttgt
    11101 gactgagttg ctattacttt ccgaagaaaa atctcccctc tttagcaaag aggggaaggg
    11161 ggagatttat tcgcttcgct cacactatgt gccgttgcta aagcaacgtt caaacgcaaa
    11221 gcgtttgtgg cagaagcaat tatcgaagaa tataagttat tcgaataagc atttacagga
    11281 aaaaatcgct ttctaccact tactcgccaa cgcccattac aaaaccactc caactgactt
    11341 acgccgttta tttgatggcg aaaatcagct tttgttttca caacaggaaa acgagcaact
    11401 tatcggcgga atttgggcaa tgcaagaggg tggtttagat gaaaaattaa cccaagccat
    11461 ttggctaggg gaacgccgtc cgcaaggcag tctagtggct caatatctct gttttcaagg
    11521 gaatttaccg caggcttgca aattgcattc cattcgcatt tctcggattg cggtattgcc
    11581 tgaaaagcaa aatcaaggcg ttggcaagcg gttgatttct cagtttattt tgcaaaattc
    11641 ggtagaaaaa cgaccgcttg attttatctc tgtgagcttt gggctaacta ctccactcca
    11701 cgcattttgg caatcttgtg gtttccaact tgtgcaaatc acaccgaata aagaggcaag
    11761 cagtggttat caaagtgcaa tgatgattta cccgatttcg gagcaaggaa agttatttgc
    11821 acagcaggca acccaagcct ttgccagaga tttcgccctt cagccatttt tcacggaaat
    11881 ttgtgaggaa ttgcaaaatt tcgaacaatt tcgaccgctt gccacacaca atttagatga
    11941 acgtgattgg aaaaatttaa ccgcgtttgc tcatggcaag cggacgtttt cagcggcgta
    12001 tgtgagttta aaacggcttt atttatcgga taaagcacgc ttttcagagc tggatatttt
    12061 tgaacgaaaa gggcaaaaaa tctccaaaat ggaggtggaa aattatcgaa aactcgtttt
    12121 gcaaaactta aacaataaat catttaaaat aatttatagg ttttattaca tttttttgtg
    12181 tttttgctct tgctaaatct gcaatttaat cgtataaatt cactgtttat ttttcatttt
    12241 gatgaggaac tccaatgtca atgtttagtc acattcaagc tgctccggca gactcaattt
    12301 taggcttagg tgaagcgttt aaagcagata gccgtgaagg taaaatcaac cttggtatcg
    12361 gtgtttatat gaccgatgaa ggcaaaaccc cgattgtcaa agcggtaaaa gaagcagaaa
    12421 aacgtttatt agagacggaa aacagtaaaa attatttaac cattgacggt gttcaagcat
    12481 tcaatgcggc aacgcaagcg ttattatttg gcgaaggggc tgaagtgatt acctcaggtc
    12541 gtgctaaaac tgcacagagt ttaggtggga caggggcatt acgcattgcg gcggaattta
    12601 tcaaacgtca tacgaatgcg aaaaatgtat ggatttcaac cccgacttgg ccaaaccata
    12661 acggcatttt tgatgcagta ggcgtgaatg taaaaggcta tcgctactac aataaagaga
    12721 ctaacggttt agattgggaa aatttaattg ccgatttaag ccaagcggaa gccggagatg
    12781 tggtgttatt gcacggttgc tgccacaacc cgaccggaat tgacccaaca cctgcacaat
    12841 gggaacagtt agcagcatta tctgccgaaa aaggctggtt gccgttattt gactttgctt
    12901 atcaaggctt tgcaaacggt ttagaggaag atgcttacgg tttacgagct tttgtgaaaa
    12961 ataaccgtga attgttggtc gcaagctcat tctcgaaaaa ctttgggtta tataacgagc
    13021 gtgtcggtgc atttacccta attgcagata atgctgatga tgccaaccgt gctttcactc
    13081 aaatcaaatc gattattcgt gtactttact ctaacccatc tgctcacggt gcaagtgcag
    13141 tagcagttgc attggctgat cctgacctta aagcacaatg gattgcggaa ttagatgaaa
    13201 tgcgtaaccg tattaaagaa atgcgtagcc aattagtgca gaaattaaaa gaaaaagggg
    13261 caaacaaaga cttctctttc attactgagc aaaatggtat gttctcattc agtggcttaa
    13321 caccggagca agtggataag ttaaaagatg agtttgcaat ttatgctgtg cgttcaggca
    13381 gaatcaatgt agcaggcatt accagcaaaa acattgatgc tcttgccgat gcgattgtga
    13441 aagtgcttta attaaacctt aaagatatat agaggcataa gcctgagtgc ttgtgcctct
    13501 tttttatctt ttaggtacaa gtgtggacac ttgcaccaac gttgattatt cgtagaacta
    13561 ggtttccaac caaagcataa aatctgctac cgtatgaaat gagccgaaca ccagcacaat
    13621 atcattttcg ttggctgttt caaacaatac tcggcttgcc tgtgccacat tttcatagct
    13681 acaagcggtt gaatttggca aaacgtttac caatttcgct aacaccgcct cgcctgtttg
    13741 tccacgccaa caatctagcc ctgcacaatg ccattcatca ataatattag ctaatggctc
    13801 gacaataccg gataaatcct tatcttctaa cgcactaaac accgcataga ttttttgatt
    13861 tggttgcttt aactcccgca gtttgtcagc taaataacgt gctgcgtgtg ggttatgccc
    13921 tacatcaata attacttttg cagccggttt aggttgagaa aaatgagtaa aatcggtatc
    13981 cgtcagggtt tgaaaacgcc ctgtcatttg agcttcattt aaggcttgtc gaataatctt
    14041 ttcatcaatt tcaaatggca gttgcattaa tactgctaat gcggtgccgg cgtttggaat
    14101 cggaataagc gggattggta aatcggtaaa ggatttttct tcagaatacc aatgaaacag
    14161 attattctta atttcaaact gccagtcttt accccggtaa aaagcgtgac aatttaacga
    14221 atcagcaatc tgtttaatcg aatgagggca atcaggctcg ccgatgacaa ccggaatatt
    14281 ttcacggaaa atgccggctt tctcacgccc gatgtcttca cggttatcgc ctaaaaatgc
    14341 aacgtggtcg atatcaatcg agataatcac ggcaaggctt ggatcaataa tattagtggc
    14401 atctaatcgc ccacctaatc cgacttctaa aatcacaata tcgagctgat tttgtttgaa
    14461 tagctcaaat gcagacagcg tactaaactc aaaataagtg agtgaagcgg tcttttttgc
    14521 attaattttt gcaaatgctt ggacgtggtc ggcatcaggt aatacttcgc cgttaattcg
    14581 cacccgttca ttatagtgaa ttaagtgcgg tgaagagtaa acaccaaccc gaaatccggc
    14641 ttttaataag gtaacttcta acaaacggca ggtcgaaccc ttgccgttag taccggcaac
    14701 ggtaatcaca taaggggcag gcttgagtaa atccagctct gttgcgacct tacgaatacg
    14761 atctaagccc atatcaatcg gtttagaatg ggcttgttct aaataggaaa gccacgtttc
    14821 caaagaatcc gtggctttag gtatgcttaa tgtattcatt cagatatttt attctgcgtt
    14881 ttctacaacc aattccgcca ctttaaacgg cgttggttga ttggttaatt ttgcacaaag
    14941 acgggaaagg gtatcacgca tctctttacg atgcacgatc atatcaactg ccccgtgttc
    15001 taataagaac tcggcacgtt ggaaaccttc cggcagtttt tcacgtacgg tttgctcaat
    15061 tacacgagga ccagcaaagc cgattagggc ttttggctca gcaatattca catcgcccag
    15121 cattgccaaa cttgcggaaa ccccacctaa agttgggtcg gttaatacag aaataaacgg
    15181 tacgccctgc tctttcattt tggctaaaat cgcactggtt tttgccattt gcattaaaga
    15241 gaataacgcc tcttgcatac gagccccgcc cgatgccgaa aaacagataa acggaatttt
    15301 ttcagctaaa gctttttccg cagctttcac aaattttgca cctactacag accccattga
    15361 accgcccata aactcaaagt taaaggaggc cgtaaccacc ggcatattat gcaatgtacc
    15421 ggcaagcacg ataaaagaat ctttctcacc ggtttgtttt tgcgcggcag ttaaacgatc
    15481 tttatatttc tttaaatctt taaatttcaa cacatcttcc ggctctaagt cggcagcgat
    15541 ttcgaccgca gaatctttat ctaacaaatt caataaacgg gtgcgggcat caatacgcat
    15601 atgatgatcg cattttggac aaacctgttg gttgcgttgt aattcttcgc tatagagaac
    15661 ttgttcacaa cttgtgcatt ttgtccatac accttccggc acttttgatt ttgcattgct
    15721 tgatgatgaa gagtctctac ctaaaattct gtctaaccag ctcattatta ttccttttgg
    15781 attgagttgt tgggttcact aaatggttgc tattaaagca taatttatcg gtttttgata
    15841 tgttttcttg acatatcaga tcactaacta tgtgtataag ttcgataaaa aaacgcaatc
    15901 agaagaatga ttaacatcac cacaatttga attaagcgtt ttttggttaa tttttccgct
    15961 gccattgctc tgctcatact taataaattt ccgcattata agcgaaaaag cacgggctat
    16021 gtagaatttg caaaattttg atcaaaaata accgcttgta aaaaggaaat actatggcaa
    16081 ccattgaaca atttcttgag gttgtaaacc aacttcgtca ccctgaacac ggctgcccgt
    16141 gggatctcaa acagaatttt gacacgatgt tccccactta ttggaagaaa cctacgaagt
    16201 agcggaagcg attcacaccc aagatcgcac tgccttgcgt gaagagttag gcgatttatt
    16261 actgcaagtg gtatttttaa gccaattagc gaaagaggaa ggcagtttca cttttgatga
    16321 tgtagtaacg gatattcacg ataaactgat ttaccgccac cctcacgttt ttggtgatgt
    16381 gaaagctact aacagcgaag aggccttaca aagttgggaa gcacaaaaag cgaatgatgc
    16441 aaaacaccgt agccaagatt ctatttttag atgatttacc ttttgctttg cctgctctta
    16501 cccgtgcaaa taagctacaa aaacggtgtg caaaagttgg attcgattgg gataaccctc
    16561 acgatgtgct ggcaaaagtc gaagaagaac tcgatgaagt caaagcagaa atcgcacaat
    16621 atccggctcg ctccatcgag cttgctgaag aattaggcga tctcttgttt gcgattgtaa
    16681 atttatgccg ccattatcat accgatgccg aggaaatttt acgcaatgca aatctaaaat
    16741 ttgaacatcg ctttcgaaaa gttgaaaaat ctgtgaaaaa atcgggaaga actgtaaaag
    16801 aatgtaaatt agaagaacta gatgtaattt ggcacaaaat aaaagtggta aaata
//
LOCUS       DANLUQ010000045        16619 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.045, whole genome shotgun sequence.
ACCESSION   DANLUQ010000045 DANLUQ010000000
VERSION     DANLUQ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16619
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.045"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(107..622)
                     /locus_tag="PXB27_001130"
     CDS             complement(107..622)
                     /locus_tag="PXB27_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL5345930.1"
                     /translation="MAGINKVIIVGNLGNDPEMRTMPNGEAVANISVATSESWTDKNT
                     GERREVTEWHRIVFYRRQAEVCGQYLRKGSQVYVEGRLKTRKWQDQNGQDRYTTEIQG
                     DVLQMLGSRQGNDMGGQQGGWGNSAPAGNSYNQGNSNYGYNQTQSAAPQSKPAPQPEP
                     AMNNFDDDIPF"
     gene            777..3605
                     /gene="uvrA"
                     /locus_tag="PXB27_001131"
     CDS             777..3605
                     /gene="uvrA"
                     /locus_tag="PXB27_001131"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDL5345931.1"
                     /translation="MQHIDIRGARTHNLKNINLTLPRDKFIVITGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTVT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTISQMVDRVLEEPEGKRLMLLAPVVKERKG
                     EHVKLLENLTASGYLRARIDGEICDLSDPPALELQKKHTIEVVVDRFKVRADLATRLA
                     ESFETALDLSGSTAIIADMDDPNAEEIVFSSSFACPHCGYSLHELEPRLFSFNNPAGA
                     CPTCDGLGVEQFFDAKKVVQNEEVSLAAGAIKGWDRRSFYYFGLLKAVAEHYGFDLET
                     PFGQLPKKYQNIILNGTKEEIEFVYVNDRGDKVKRKHAFEGVLNNMARRYKETESNSV
                     REELAKYINNRPCMDCEGSRLRREARYVFLDKTNLPMVSEKSIGEALTFFEELKLAGQ
                     KEKIAEKILKEIRERLQFLVNVGLNYLSLSRSAETLSGGEAQRIRLASQIGAGLVGVM
                     YVLDEPSIGLHQRDNERLLNTLVHLRNLGNTVIVVEHDEDAIRAADHIVDIGPGAGVH
                     GGQVIAQGTAAEIMQIEESITGQFLSGKQKIEIPAQRVPYEPTKLLTLSGASGNNLKK
                     VKLEIPVGLFTCITGVSGSGKSTLVNDTLFPLAQNALNRAENSDVAPYESIDGLSHFD
                     KVIDIDQSPIGRTPRSNPATYTGLFTPIREIFAGTQEARARGYNVGRFSFNVRGGRCE
                     ACQGDGVIKVEMHFLPDVYVPCDHCKGKRYNRETLEIRYKGKTIHQVLEMTVEEAREF
                     FAPMPMIARKLQTLMDVGLSYIRLGQSSTTLSGGEAQRVKLATELSKRDTGKTLYILD
                     EPTTGLHFADIKQLLGVLHRLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGEI
                     IATGTPEEVAKDKRSHTARFLKEILAKG"
     gene            complement(3697..5430)
                     /gene="dauA"
                     /locus_tag="PXB27_001132"
     CDS             complement(3697..5430)
                     /gene="dauA"
                     /locus_tag="PXB27_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HDL5345932.1"
                     /translation="MLNKWFLTKNAFLAVKPFSALKDALNAGYGKQHLIRDIISGLTV
                     GVIAIPLSMALAIASGVPPQHGLYTAIVAGIVIALTGGSRFNISGPTAAFVVILYPVT
                     QQFGLSGLLMATMLSGGILVLMALLRLGRLIEYIPLPVTLGFTCGIGIVIATLQIKDF
                     LGLSIEKMPEHYLGKLEAIFSALPTINWADTLVGVVTLLVLTRWHKLRLPIPGHLPAV
                     FIGTLLSLLLIHFGQNVATIGSAFHYTLSDGTTGYGIPSVLPEFVLPWNLPNAEGNRI
                     DWNLTTIQALLPAAFSMAMLGAIESLLCAVVCDNMTDTKHHSNNELLAQGLGNIASPF
                     LGGITATAAIARSAANVRSGAVSPISSVVHALLVLFALLFLAKALSYLPLSSMAALLL
                     VVAWNMADIPQIIQLTRRSGKNEIAVLLTCITLTVLFDMVIAITVGVLLASLLFIRTI
                     AEMTKSFEMAHPTDLNDVLVYRISGPLFFAAADNLFADLHNKTVHTDHQIKHIVLQCD
                     AVTVLDAGGIHALTRFVQHMLPHQQLHLCNVQFQPMRMLVRSQKTVNEIPQIQFSSDL
                     ESAFDKIRQAN"
     gene            5672..6604
                     /locus_tag="PXB27_001133"
     CDS             5672..6604
                     /locus_tag="PXB27_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5345933.1"
                     /translation="MDSLIHFLSAFLTNITWIGALELGLIYSLVALGVLISYKILDFP
                     DLTADGSFPLGGAVCVISILNQVDPWLATLYGTLAGAVAGMVTASLHIGFKIEKLLAS
                     ILMMIALYSINLRIMGMPNIAILGETTIYDVMPIESDLQLALVRLVMALLVVIIVKGL
                     FDLFFATEIGLAVRATGTNSRMAKAQGIAINKMTILGMAISNALIALGGALYVQSNGG
                     VDISIGVGTIVIGLAAVIIGEAIFSVKRIIWLTFAVIIGSILYRFFIALALNNETLNG
                     IGFGPQDLNLITALLVVIVLAVPRIKHKLLGKRG"
     gene            6606..7403
                     /locus_tag="PXB27_001134"
     CDS             6606..7403
                     /locus_tag="PXB27_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5345934.1"
                     /translation="MIELNNLFITFNKGTAIENPVLRGLSLKVEQGEFVSVIGSNGAG
                     KSTLLNAISGDCAVDSGEILIQGNKVNQSTTWQRANQVARVFQDPMAGTCESLTIEEN
                     MALAYQRGRKRGLGFAIKRQMRELFKDKLALLGLGLENRLTDQMGRLSGGQRQAVSLL
                     MASLQPSNILLLDEHTAALDPKTTDFVLELTNKIVREQKLTTLMVTHSMRQALDYGDR
                     TVMLHQGQVAFDVSGEQRQKMDVPDLLQLFQQSRHEQLSDDGLLLGN"
     gene            7490..7711
                     /locus_tag="PXB27_001135"
     CDS             7490..7711
                     /locus_tag="PXB27_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345935.1"
                     /translation="MLSIFKTPVEKETLDDWAKISVDVAKVAILAIPVILFGKEPTLI
                     KLTNLALLVLSIYVFLTLGRKLRQLKEVL"
     gene            7894..9819
                     /locus_tag="PXB27_001136"
     CDS             7894..9819
                     /locus_tag="PXB27_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HDL5345936.1"
                     /translation="MTKYDDKIQHAFSENGLLSQNISGFRPRQAQLEMAQVVAKAVKF
                     ATPVVVEAGTGTGKTFAYLVPALLSGKKTILSTGSKNLQDQLFNRDLPTIQKALKYKG
                     KIALLKGRANYLCLERLDQVTAMGVLGDKSVLTDLKKVSKWQNATKTGDLSECITIAE
                     DSPILPQLVSTTESCLGSDCPHFKDCYVVQARRKAMEADVVVVNHHLFCADMAVKKTG
                     FGELIPEAELVIFDEAHQLPDIASQYFGQSLSSRQLFDICKDTNIVYRTELKDLSQLG
                     KAADHLTKVVQDFRLLMGGEGQIRGNLRELFRDKKVVDGLVKVAENIDFLSEVAKKSL
                     GRSETLDKIFERLAEVKVQLKKLMDTSVTGYCYWYEANGRSFGLHITPLMVADKFGEQ
                     LKNQQIGWVFTSATLEVGGKFDHFCQRLGIENAEQLVLQSPFDYQHQSLLCVPRYLPD
                     TNKSNTLTALGQLLKPVIEANKGRCFLLCTSYYMMRGLADFLREHSSLNVLLQGETSK
                     SRLLEKFVKEDHSVLVATQSFWEGIDVRGDDLSLVIIDKLPFTSPDEPLLKARMEDCK
                     IQGGDPFNDIQIPEAVITLKQGVGRLIRDVTDRGVVIICDSRLVMRNYGQTFLKSLPP
                     SRRTRDLGNVIQFLKQK"
     gene            9893..10258
                     /locus_tag="PXB27_001137"
     CDS             9893..10258
                     /locus_tag="PXB27_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDL5345937.1"
                     /translation="MLSTLLRMVASLALLFVMLYLGKLIVYLFPIGIPDSILGMLILF
                     GCLVIGIVKVEWVIPSAKPLTRYITLFFLPICVELIEHLELLRQNLNTFVLATALSTL
                     VSVVAIGVFAQWIFHYKRG"
     gene            10260..10946
                     /locus_tag="PXB27_001138"
     CDS             10260..10946
                     /locus_tag="PXB27_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDL5345938.1"
                     /translation="MSYLYTVLTVIMFIAGLKISQKLKSSFLNPFLIALALLIGVLLI
                     FNIPYQDYYQGNSPLNNLLGVSVVALAYPFYEQLPHIRKKWRQIVVILLFSTIITMIT
                     GLGFSLLFGASQEILAAILPKSVSMPMAIIITNEINGNVAIAAVGVMIAGLVGSILGF
                     TLLRLAKVRNVMVIGLSMGAASHALGTARCLEHSMKAGSYSSIALVACGVLSSIFAPL
                     IFNVIVTLCY"
     gene            11030..12793
                     /gene="msbA"
                     /locus_tag="PXB27_001139"
     CDS             11030..12793
                     /gene="msbA"
                     /locus_tag="PXB27_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HDL5345939.1"
                     /translation="MQEQITQEKDLSTLKTFKRLWPTISPFKAGLITGAVALVLNALV
                     DSSLIYLLKPLLDEGFGQANNDFLKLMAVLVVVFILVRGITNYIASYCLAWVSGKVVM
                     TLRRHIFQHLMYMPVSYFDNNPTGRLLSRVTYDTEMVASSASHSLVTIVREGAYLLSL
                     FVVMIYTSWQLSIVLFVMAPIIGVLISFVSKYFRQLSRNIQSSMGELTVTTEQMLKGH
                     KVVLSFGGQEIEKARFDRVSNDMRRKGMKVVSADGISDGVVQLIASFALSAVLYVATF
                     PEVMSENLTAGSFTVVFSSMMAMLRPLKSLTNVNSQFQRGMAACQTLFEFLDLKTEKD
                     HGLISVAKAEGNVEFKKVSFSYEGKEEKALDNISFTIPKGKTVALVGRSGSGKSTIAN
                     LLTRFYDVTDGQILLDGINIQDYRLKNLREQCSVVSQQVHLFNDTIANNIAYAAIDKY
                     SREEIIAAAKAAHAMEFIEKLENGLDTVIGENGASLSGGQRQRLAIARALLRNSPVLV
                     LDEATSALDTESERAIQSALEVLQKDRTVLVIAHRLSTIEKADEILVVDHGNIIERGS
                     HTELLAQGGAYKQLHSLQFGA"
     gene            12882..13514
                     /gene="lolB"
                     /locus_tag="PXB27_001140"
     CDS             12882..13514
                     /gene="lolB"
                     /locus_tag="PXB27_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HDL5345940.1"
                     /translation="MKKLTKLLSLSALLVLTACNSVLNASTEVQQPTVQIPHDDPQWQ
                     QHLAKLSQIQGYTATGQFGYISPEERFSSHFNWRYTNPIQFGLELSSNLSTKSLKLER
                     NKRGLTVSDNEGNSRSDRDVDALMQEIIGVSFPIDQFAYWVKGLPEKDGNYIVNEKRQ
                     LSQFSYPLNGTLWKASYVEYHEDRVPNLPKLIVLENGTQTLKIRIEKWAY"
     gene            13502..14356
                     /gene="ispE"
                     /locus_tag="PXB27_001141"
     CDS             13502..14356
                     /gene="ispE"
                     /locus_tag="PXB27_001141"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDL5345941.1"
                     /translation="MGVLMKKVTLPSPAKLNLFLYITNKRADGYHELQTLFQFLDFGD
                     EIEIEITESSEVELINPVEGVATEENLIYRAAKLLQKTTACRQGCRIGIKKNLPMGGG
                     VGGGSSNAATVLVGLNHLWNTELSLEKLAELGLSLGADVPIFVRGMAAFAEGVGEVLT
                     PCDPQEKWYVVLKPEVSISTAAVFTDPNLPRNTPKRPLAELLSLPWENDCEKVVREKY
                     SAVEDLIQELLQYAQFRLTGTGACIFAEFETKAEAEQVFAHKPEAVFGFVAKGQNISP
                     LHQMLFQI"
     gene            14382..15332
                     /locus_tag="PXB27_001142"
     CDS             14382..15332
                     /locus_tag="PXB27_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HDL5345942.1"
                     /translation="MTDIKLFAGNATPELANRIAKHLYTSLGNATVGRFSDGEIQVQI
                     NENVRGGDIFIVQSTCAPTNDNLMELIVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVIADFLSSVGVDRVLTCDLHAEQIQGFFDVPVDNVFGSPVLIDDILKKS
                     DLVNPIVVSPDIGGVVRARAVAKLLNDTDMAIIDKRRPKANVSQVMHIIGDVADRDCI
                     LVDDMIDTGGTLVKAAEALKERGARRVFAYATHAVFSGTAAQNLANPALDEIVVTDTI
                     PLSNEIKALNKVRVLTLSGMLAEAIRRISNEESISAMFDA"
     gene            15410..16420
                     /gene="pyrD"
                     /locus_tag="PXB27_001143"
     CDS             15410..16420
                     /gene="pyrD"
                     /locus_tag="PXB27_001143"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HDL5345943.1"
                     /translation="MYSLIRKLLFALDTEKAHQFSIQALALAGKSPFSILPTPDNPTQ
                     VMGLTFKNPIGLAAGADKNGEAIDGFGKLGFGFIEVGTITPLAQDGNPKPRQFRIVDA
                     EGIINRNGFNNKGVDVLVENVKNATYSGILGINIGKNAVTPIEHSFDDYQICLRKVYQ
                     YADYVAVNISSPNTKNLRSLQYGEALDDLLRNLKNEQAQLSQKMGKYRPLVLKIAPDL
                     TDEEIVSVADSLIRYQIDGVIAGNTTLSRESIAGLPYSEQLGGLSGKPLHSLSTRLIS
                     KLSAELGERIPIIGCGGVHSLESGQEKVNAGASLLQVYSAMIYQGPSLIQTLAKHLQL
                     NS"
BASE COUNT         4796 a         3136 c         3829 g         4858 t
ORIGIN      
        1 gttccctatt attttgagta aggtaggggc gctttatatg agtagctttt tatttcatcg
       61 aaatgcggtg tatggaaccc atgagctact taagtcaata ctttgattag aacggaatat
      121 catcatcaaa attgttcatt gccggctcag gctgtggtgc cggtttagat tgcggtgcag
      181 cactttgggt ttggttgtaa ccataattgc tgttgccttg attgtagcta ttacccgcag
      241 gagcagaatt tccccaaccg ccttgctgac cgcccatatc attaccttga cggctgccga
      301 gcatttgtag cacatcgcct tgaatttcgg tggtgtaacg atcttgcccg ttttggtctt
      361 gccatttacg ggtttttaaa cgtccttcta cgtaaacttg agagccttta cgtaaatatt
      421 gcccgcatac ttctgcttga cggcggtaga atacaatacg atgccactcg gttacttcac
      481 ggcgttcacc ggtgttttta tccgtccagc tttctgaggt tgccacacta atatttgcaa
      541 ccgcttcgcc atttggcatt gtacgcattt cagggtcgtt acctaaatta ccgacaataa
      601 ttactttatt gataccagcc ataaaatcct ctgtttgata aattaaacta aaatttgcta
      661 agtattctac ctgaaaatcc aaaagatgaa caccaaaaaa ctgatatttt gtacagtttt
      721 attaaaatat gcgataatga tcgaaaattg agaaaatagc actggacatt aaaaatatgc
      781 aacatatcga tattcgtgga gcaagaacac acaacttaaa aaacatcaat ttaaccttgc
      841 cgagagataa attcattgta attaccgggc tttccggctc gggcaaatcg tctctggcgt
      901 ttgatacgct ctatgcggaa ggacaacgcc gttatgtaga atcactgtct gcatacgccc
      961 gccagtttct ctccttaatg gaaaagcctg atgtggatca tattgaaggg ctttcccctg
     1021 cgatttcgat tgagcaaaaa tcaacctctc acaacccacg ttcgaccgta ggaacggtaa
     1081 cggaaattca cgactattta cgtttattgt ttgctcgagt gggcgagcct cgttgccctg
     1141 accacgatgt gccattggct gctcaaacta tttcgcagat ggtggatcga gtattggaag
     1201 aaccggaagg aaaacggttg atgctacttg cccctgtggt gaaagagcgt aaaggcgaac
     1261 acgtaaaatt attggaaaat ttgaccgcta gtggctattt gcgggctaga attgacggtg
     1321 aaatttgcga tttatccgac ccaccggcat tagaattaca gaaaaaacac accattgaag
     1381 tggtggtgga tcgctttaaa gtaagggctg atctagccac ccgtttggcg gaatcttttg
     1441 aaacagcgtt agatttatcc ggctctaccg caattattgc agatatggac gacccaaatg
     1501 ccgaagaaat tgtgttttcg tccagctttg cttgtccgca ttgtggttac tctttacacg
     1561 aattagaacc acgccttttc tcgtttaaca acccggcagg tgcttgcccg acttgtgatg
     1621 gtttgggcgt tgagcagttt tttgatgcca aaaaagtagt gcaaaatgaa gaggtttcat
     1681 tagccgccgg tgcgatcaaa ggctgggatc gccgcagttt ctactatttt ggcttattaa
     1741 aagccgttgc cgagcattat ggctttgatt tagaaacccc attcgggcag ctaccgaaaa
     1801 aatatcaaaa cattatttta aacggcacta aagaagagat tgagtttgtt tatgtcaatg
     1861 accgaggcga taaggtcaaa cgtaaacacg cttttgaagg ggtgctgaat aatatggctc
     1921 gccgctataa agaaaccgaa tcaaactcgg tgcgtgaaga gttggcgaag tatattaata
     1981 atcgtccttg tatggattgc gaaggctcac ggttacgccg tgaagcccgt tatgtattct
     2041 tagataaaac caatttgccg atggtttcgg aaaaaagtat cggcgaggca ttaacctttt
     2101 ttgaagagct gaaattagcc ggtcagaaag aaaaaattgc tgagaaaatc ctcaaagaaa
     2161 ttcgtgaacg gctacagttt ttagtgaatg tcggcttgaa ttatctttct ctttcccgct
     2221 cggcagaaac cctttcaggt ggcgaggctc agcggattcg ccttgccagc caaattggtg
     2281 cgggtttagt aggggtaatg tatgtgctgg atgagccttc catcggcttg caccaacgag
     2341 acaatgaacg cctactgaat actttggtgc atttacgcaa tttaggcaat accgtgattg
     2401 tggttgaaca cgatgaagat gcgatccgag ccgcagatca tatcgtggat ataggcccgg
     2461 gtgccggtgt acacggtggg caagttatcg cacaaggtac ggcagctgaa attatgcaaa
     2521 ttgaggaatc tattaccgga caattcctct caggcaagca aaaaattgag attccggctc
     2581 agcgtgtgcc ttatgagcca actaaattgc tcaccttatc gggagcaagt ggtaacaact
     2641 tgaaaaaagt gaaattagag attccggttg gtttatttac ttgtatcacg ggtgtatcag
     2701 gttcgggcaa atcaacttta gttaatgaca cactattccc tctggcacag aatgcgttaa
     2761 accgagcgga aaacagcgat gttgctcctt atgaatcgat tgacggctta tcgcattttg
     2821 acaaggtaat tgatattgac caaagcccga ttgggagaac tcctcgctca aatccggcaa
     2881 cttacaccgg attatttacg ccaattcgtg agatttttgc cggcacgcaa gaagctcgag
     2941 ccagagggta taacgtaggg cggtttagct ttaatgtgcg gggcggacgt tgcgaagcct
     3001 gtcaaggtga cggcgtgatt aaagttgaaa tgcacttctt gcctgatgtg tatgtgcctt
     3061 gcgatcactg taaaggcaaa cgctataacc gtgaaacctt ggaaattcgc tataaaggca
     3121 aaactattca ccaagtgtta gagatgacgg tggaagaggc gagagaattt tttgccccta
     3181 tgccgatgat tgcccgcaag ttgcaaacct tgatggacgt ggggttatct tatatccgtt
     3241 tagggcaatc ttctactacg ctctcaggcg gtgaagccca acgggtgaaa ttggcgaccg
     3301 agctttccaa acgagacacc ggcaaaacgc tctatatttt agatgaaccg accaccggct
     3361 tgcattttgc cgatattaaa cagttactcg gcgtgctaca tcgcctgcgt gatcaaggca
     3421 atacgattgt ggtgatcgaa cataatttag atgtgattaa aaccgcagac tggattgtcg
     3481 atcttggccc tgaaggtggc agtggcggcg gtgaaattat tgctaccggc acaccggaag
     3541 aagtggcaaa agacaaacgt tctcacaccg cacgcttctt aaaagagatt ttggctaaag
     3601 gctaacgttc aacacaagcg gtcatatttt cggaaaatgt tgcaatttgc aaaaagttgg
     3661 aaaatatgac cgcttgtttt atatccggag ttattcctag ttagcctgtc tgattttgtc
     3721 gaaggcagat tcaagatcgc tactgaattg aatttgcggg atttcgttta ccgttttttg
     3781 tgagcggact agcattctca tcggctgaaa ttgcacatta cagagatgaa gttgttgatg
     3841 tggcaacata tgctgtacaa agcgggttag ggcgtgaatg ccaccagcat ctaatactgt
     3901 aacggcatcg cattgcagca caatatgttt aatttggtga tcggtatgta cggttttatt
     3961 gtgcagatcg gcaaagagat tatcggcggc agcaaaaaat aacggaccgc tgatccgata
     4021 aaccaacaca tcgttcaaat cggttgggtg agccatttca aaggatttag tcatttctgc
     4081 aatagtacgg ataaatagca ggcttgccag taacacccct accgtaatgg caattaccat
     4141 atcaaataat accgtgaggg taatgcaagt cagtaaaacc gcaatttcat ttttacctga
     4201 acggcgggta agttgaatga tttgtggaat atctgccata ttccaagcta ctaccaataa
     4261 tagtgcagcc attgaggaaa gaggcaaata ggaaagtgct ttagccaaaa agagtagggc
     4321 aaataaaacc aacaaggcgt gaaccacact agaaatgggg gaaactgccc cggaacgaac
     4381 attggcagcc gatctcgcaa tcgctgctgt ggcggtaatt ccccctaaaa atggcgaggc
     4441 aatattacct aagccttggg ctaatagttc gttgttagag tggtgcttgg tatctgtcat
     4501 attatcgcac actaccgcac ataataacga ttcgatagcc ccaagcattg ccattgaaaa
     4561 ggcagccggt aataaggctt gaatggtggt taagttccaa tctatccgat tcccctctgc
     4621 attcgggaga ttccaaggta acacaaattc aggtaatacg cttgggatac catacccagt
     4681 ggtgccgtca gagagcgtgt agtggaatgc tgagccgata gtggcaacgt tttgtccgaa
     4741 atgaatcagc agtaatgata acagcgtgcc gataaagacg gcgggtaaat gcccgggaat
     4801 aggcaaccgt agcttgtgcc aacgagttaa aaccaacaat gttacgacac ccactaaggt
     4861 gtcagcccaa tttatggttg gcaatgctga aaaaatagcc tctagcttgc caagatagtg
     4921 ttcaggcatt ttttcaatgc ttaagcccag aaaatctttg atttgcaagg tggcgatcac
     4981 aataccgatg ccacaggtaa aacccagtgt gacaggtaga ggaatgtatt caattaagcg
     5041 tcctaatcgg agtaacgcca tcaatactaa aattcctccg gaaagcattg ttgccatgag
     5101 taatccactt aagccaaact gctgcgtgac ggggtataga atcaccacaa aagcggctgt
     5161 tgggcctgaa atattaaatc ttgaaccgcc tgttagggca atcacaatcc cggcaacaat
     5221 cgccgtatat aaaccgtgct gtggtggcac accgctggca atcgccaacg ccatagataa
     5281 cggaatagca ataacgccaa cggttaagcc tgaaataata tctctgatta aatgttgttt
     5341 accataacct gcattgagag catcttttaa tgcactaaaa ggcttgacgg caagaaaagc
     5401 atttttagtt aaaaaccatt tatttaacat agaatgttcg agtgaagaga atgtttcgct
     5461 tattttactg caatgaataa aattaaattt gagctaacgc aagaaaaagc aggaaagacg
     5521 aaatatgttg gagacaagcg gttgttttag gcgaaaaatt tgcaaataca aataattagc
     5581 tgattttatt ttgaaaatag cgtaaaatgc cactttaatt tgtactacta tagtagtgca
     5641 tttgtgtgat ttcattaact ataataaaca aatggacagt cttattcatt ttttgagtgc
     5701 ctttttaact aatatcactt ggattggggc gttagagctg gggttaattt attcccttgt
     5761 tgcccttggg gtattaattt cttataaaat tctggatttt ccggatctga ccgcagacgg
     5821 cagtttccct ttaggagggg cggtgtgtgt gatcagcatt ttgaatcaag ttgatccttg
     5881 gctggcaact ttatacggca cgcttgccgg agcagtagcc ggtatggtta ctgcaagttt
     5941 gcatatcggc tttaaaatcg aaaaattgct tgccagtatt ttaatgatga tcgcactcta
     6001 ttcgattaat ctacgcatta tgggaatgcc gaatattgct attttaggcg aaaccacgat
     6061 ttacgatgta atgccgattg aaagcgatct acagttagcc ttagtacgat tagtgatggc
     6121 attgctggtt gttattatcg taaaaggttt gtttgacctc ttttttgcga ccgaaatcgg
     6181 tttggctgtg cgggcaaccg gtactaatag tcgaatggcg aaagcacagg gtattgcgat
     6241 taataaaatg acgattttag ggatggcgat ttctaatgcg ttaattgcct taggtggtgc
     6301 tttgtatgtg caaagcaacg gtggtgtaga tatttcgatt ggggtaggaa ctatcgtgat
     6361 tggcttggcg gcggtaatta tcggggaagc aattttttcg gtaaaacgga ttatttggct
     6421 tacctttgcg gtgattattg gctcaattct ttatcgtttc ttcatcgcac tagcattaaa
     6481 taacgaaact ttaaacggca ttggctttgg cccgcaagat ctgaatttaa ttacggcgtt
     6541 attggtggtg attgtattag cggttcctcg aattaaacac aaattgctcg gtaagcgagg
     6601 ttaatatgat tgaactcaat aacttattta ttaccttcaa caaagggaca gcaattgaga
     6661 accctgtgct gagaggctta tccctaaaag ttgagcaagg cgaatttgtg tcggtaattg
     6721 gtagcaatgg ggcgggtaaa tctaccttat taaatgcgat cagtggtgat tgtgcggtgg
     6781 actccggtga gattttaatt caaggaaata aggtaaacca atccaccact tggcaacggg
     6841 caaatcaagt cgcccgagtt ttccaagacc cgatggctgg cacttgtgaa agtttaacga
     6901 ttgaagaaaa tatggcactg gcttaccaac gtggacgtaa acgtgggttg ggctttgcga
     6961 tcaaacgcca aatgcgagag ttattcaaag acaaacttgc tttgttaggg ctaggtttgg
     7021 aaaatcggct tactgatcaa atggggcgtt tatcaggcgg gcaacgccaa gcggtgagct
     7081 tgctgatggc atcacttcag ccgtctaata tcttattgtt agatgaacac accgcggcac
     7141 tggatccgaa aaccaccgat tttgtgcttg aacttaccaa taaaattgta agagaacaga
     7201 aattaaccac gctaatggta acgcattcaa tgcggcaagc ccttgactat ggcgatagaa
     7261 ccgtgatgtt acaccaaggg caagtggcgt ttgatgtttc gggcgagcaa cggcaaaaaa
     7321 tggacgtgcc ggatctgtta caacttttcc aacaaagtcg ccacgagcag ttgagtgacg
     7381 atggattatt actcggcaat taattttgcg atctgcatcg aaaaaatgaa tttctccttt
     7441 tgtttttggc tgtcaaattt taaataataa gcccaaaagg aggtattcaa tgttaagcat
     7501 tttcaaaaca ccggtagaga aagaaacgct agacgattgg gctaaaattt cggttgatgt
     7561 agcgaaagtt gcgattttgg caattcctgt tatacttttt ggcaaagaac caacgttgat
     7621 taaattgact aacttagcat tattggtatt gagtatttat gtttttttga cgcttgggcg
     7681 aaaattacgg cagctaaagg aggtgttgtg atggaactag gctggaattt aaccataggt
     7741 ttgctaattt taaacgtctt tgcgtttgct tggctttata ttattaaccg gatttctaaa
     7801 gaacaattat ccaaaaatca ataatattta atttttcaca agcggttgtt tttgcaaaat
     7861 attttgtaaa ttcgaccgct tgcctgtttt taaatgacaa aatacgacga taaaattcag
     7921 catgcattta gcgaaaatgg gttgctcagc caaaatatca gtggatttcg accacgccaa
     7981 gcacagcttg aaatggctca agtggtcgca aaagcagtaa aatttgcgac acctgttgtg
     8041 gtagaggcgg gaaccggcac aggcaaaacc tttgcttatc ttgtgccggc actactttca
     8101 ggcaagaaaa ctattctttc gacaggttct aaaaacctgc aagatcaact ttttaatcga
     8161 gatcttccca ctattcaaaa agccctgaaa tacaaaggca aaattgcgtt actcaaaggg
     8221 cgtgcgaact atttgtgttt agagcgttta gatcaagtga ccgcaatggg cgtacttggg
     8281 gataaatccg ttcttaccga tttaaaaaaa gtcagtaaat ggcaaaatgc cactaaaaca
     8341 ggggatttaa gtgaatgtat taccattgcc gaagatagcc cgattctacc gcagttagtt
     8401 agcaccaccg agagttgctt aggctcggac tgcccgcatt ttaaagattg ttatgtggta
     8461 caagcccgcc gtaaagcgat ggaggcagac gtagtggtgg taaaccatca cctattctgt
     8521 gctgatatgg cagtaaaaaa gaccggtttt ggtgagttga tccctgaagc tgagctggtg
     8581 atctttgatg aagctcacca actgccggat attgccagcc aatattttgg gcaatcgctt
     8641 tcttcccgcc agttatttga tatttgtaag gataccaata ttgtttaccg cactgaactc
     8701 aaagatctgt ctcaattagg caaagcggct gatcacttga cgaaagtagt gcaagatttc
     8761 cgattattga tgggcggaga agggcagatt cgaggcaatt tgcgagagtt atttcgtgat
     8821 aaaaaagtgg tcgatggctt ggtaaaagtg gctgaaaata ttgatttttt aagtgaagtg
     8881 gcgaaaaaat cgctggggcg gtcggaaacg ttggataaaa ttttcgagcg gttagcagaa
     8941 gttaaggttc aacttaaaaa attaatggat actagtgtga caggctattg ttactggtat
     9001 gaggcgaatg gacgatcctt tgggctgcat attacgccgc ttatggtggc agataaattt
     9061 ggtgaacagt tgaagaacca gcaaattggt tgggtgttta cctcagccac actcgaagtt
     9121 gggggcaaat tcgatcattt ttgtcaacgg ctgggcatcg aaaatgcgga gcagttggtg
     9181 ttacaaagtc cgtttgatta ccaacatcaa tctttactct gtgtgccccg ctacttgccg
     9241 gataccaata aatccaatac tttaactgct ctcgggcaat tattaaaacc ggtgattgag
     9301 gcgaataaag ggcgttgttt cttgctttgt acttcctatt atatgatgcg aggtttagcc
     9361 gactttttac gcgaacacag ttcgctgaat gtgttattgc aaggcgaaac cagcaaaagc
     9421 cgtttattgg aaaaatttgt gaaagaagat cacagcgtgt tggtggcgac ccaaagtttt
     9481 tgggagggga ttgatgtgcg aggcgatgat ttatcgttgg tgattattga taaattgccg
     9541 ttcacttcac cggacgaacc attactcaaa gcccgaatgg aagattgtaa gatacagggc
     9601 ggtgatccgt ttaacgatat tcaaattccc gaagcggtga ttaccctcaa acaaggtgta
     9661 gggcgtttaa ttcgtgatgt gaccgataga ggtgtagtga ttatttgtga ttcccgtttg
     9721 gtgatgcgaa attatgggca aactttcttg aaaagtttac cgccgtctag acgcactaga
     9781 gatttgggga atgttattca atttttgaaa caaaaatagg gttacaagcg gtcagatttc
     9841 tgcagatttt tgcaaaaaaa cggataaaaa taaccgcttg ttgaggagtt atatgttgtc
     9901 tacattgctg cgtatggttg cttctttggc attgttattt gtgatgcttt atttaggcaa
     9961 gttaatcgtt tatctattcc ccatcgggat cccggatagt attttaggta tgcttatctt
    10021 attcggctgc ttggttatcg gcattgttaa agtggaatgg gtgataccga gcgcgaaacc
    10081 gctcacacgt tatataacac tatttttcct tccgatttgt gtcgaactca tcgaacatct
    10141 tgagctgtta cgccaaaatc tgaacacttt tgtattagca acggcattaa gcaccttagt
    10201 ttctgtggtc gcgattggcg tgtttgcaca gtggattttt cattacaaga gagggtaaaa
    10261 tgagctatct ttacactgtg ttgacggtaa tcatgtttat tgccggtttg aaaatcagcc
    10321 aaaaactcaa gagcagtttt ctgaaccctt tcttaattgc actcgctttg ctaattggtg
    10381 tattactgat ttttaatatc ccttaccaag actattatca aggtaattcc cccttgaata
    10441 acttacttgg cgtttctgtt gtggcacttg cttacccttt ttatgaacaa ttaccccata
    10501 tccgtaaaaa atggcgacag attgtcgtga ttttgctgtt tagtaccatc attacgatga
    10561 taaccgggct tgggttttca ttattatttg gggcaagcca agagatctta gccgccattt
    10621 tgcctaaatc ggtatcaatg ccaatggcaa ttattattac gaatgaaatt aatggaaatg
    10681 ttgcaatcgc agcggttggg gtgatgatcg caggcttagt gggatctatt ttaggcttta
    10741 cgcttctgcg cctggcaaaa gtaagaaatg tgatggtgat tgggttaagt atgggagctg
    10801 cttcccacgc attaggcacg gcaagatgct tagagcatag tatgaaagcc ggtagctata
    10861 gttctatcgc attagtggct tgtggtgtgc tgtcttctat atttgcacct ttaatattta
    10921 acgttatcgt cacgttatgt tactaaaaac agtgaaatct caaaccttat ttggtaactg
    10981 gctgaaatca aggtacaata ccgtgatttt tcaatgaaaa tagagaatta tgcaagaaca
    11041 aataacacaa gaaaaagatc tttctacgct taaaaccttt aagcgtttat ggccgactat
    11101 cagcccgttt aaagcgggtc tgattacagg tgctgtcgct ttagtattaa atgcgttagt
    11161 ggattctagt cttatctatc tcctcaaacc cttgctagat gaaggctttg ggcaagctaa
    11221 taatgatttt ctgaaattga tggcggtgct tgtagtagtt tttattttag tgcgcggtat
    11281 taccaattat attgccagtt actgtttagc ttgggtgtca ggcaaggtgg taatgacatt
    11341 acgccgccat atttttcaac atttaatgta tatgccggta agctattttg ataacaaccc
    11401 gaccgggcgt ttgctctcac gagtgactta cgataccgaa atggtggcga gttctgcttc
    11461 tcattcttta gtgactattg tgcgtgaagg ggcttatctg ctgtcattgt ttgtggtgat
    11521 gatctatacc agttggcagc tttctattgt gttatttgtg atggctccta tcattggggt
    11581 actgatcagt ttcgtttcaa aatattttcg tcaattaagt cgcaatatcc aaagttcaat
    11641 gggggagctt actgtcacga cagaacaaat gcttaaaggg cataaggttg tactttcctt
    11701 tggtgggcag gaaattgaga aagcacgttt tgatcgagtc agtaatgata tgcgtcgtaa
    11761 agggatgaaa gtcgtttctg ctgatggcat ttctgatggt gtggtacagc ttatcgcctc
    11821 ttttgcgtta tctgctgtgc tttatgttgc gaccttcccc gaggtgatga gtgagaattt
    11881 aaccgcaggt tcattcacag tagtgttttc ttcgatgatg gcaatgttac gtcctctaaa
    11941 atcactcacc aatgtgaact cgcaattcca acgtggaatg gcagcctgcc aaacgttatt
    12001 tgaattttta gatttaaaaa ctgaaaaaga tcacggctta atctccgttg ctaaagctga
    12061 aggcaatgtg gaatttaaaa aggtaagctt tagttatgaa ggtaaagagg aaaaagcact
    12121 cgataatatt tctttcacca ttcctaaagg caaaacagta gcattggttg ggcgttccgg
    12181 ctcgggtaaa tcaactattg ccaatttgct tacccgtttt tatgatgtga ctgacggtca
    12241 aattttgctt gatggaatta atattcaaga ttaccgctta aaaaacttaa gagaacaatg
    12301 ctcggtggtt tctcaacaag ttcatttgtt taacgatact attgctaata atattgctta
    12361 tgctgcaatc gacaaataca gccgagaaga aattattgct gctgcaaaag ctgcacacgc
    12421 aatggaattt atcgaaaaat tggaaaatgg cttagacact gtaattggtg aaaatggcgc
    12481 aagtctttcc ggtgggcaac gccaacgctt ggcaatcgct cgtgcattac tgcgaaattc
    12541 tcctgtgctt gtgctggatg aagctacctc tgcattagat acggagtcag aaagagcgat
    12601 ccaatctgca cttgaagtgc tacagaaaga tcgtacggtg ttggtcatag cacaccggtt
    12661 atcaacgatt gaaaaagccg atgaaatttt ggtagtcgat cacggaaata ttattgagcg
    12721 tggcagccat acggaattgt tagcacaagg tggggcttat aaacaactcc atagtttaca
    12781 attcggagcc taatttgtag gggagaaata tgtttctccc caattaatga taaaaacagg
    12841 gcgaaaaata tttcgtccct acaataattt atttggatac tatgaaaaaa ctgacaaaac
    12901 ttttatctct tagtgcttta ctggtcttaa ccgcctgtaa ttcggtacta aatgcctcaa
    12961 cagaggtgca acaaccaaca gtacaaattc cgcatgacga tccacaatgg cagcagcatt
    13021 tagcgaaact gagccaaatt caaggctata ctgcaacggg gcaatttggc tatatttcgc
    13081 ctgaagagcg tttttcttcc catttcaact ggcgttatac taatccaatc caatttgggt
    13141 tagagctttc ctcgaatctc tcgactaaat cactgaaatt agagcgtaat aaacgaggct
    13201 taacggtttc ggacaatgag ggtaatagcc gttctgaccg tgatgttgat gcgttaatgc
    13261 aagagattat cggtgtttcg tttcctatcg atcagtttgc ctattgggta aaaggcttgc
    13321 ctgaaaaaga tggcaactat attgtgaatg aaaaacgcca gctgtcgcaa tttagttatc
    13381 cgcttaatgg cacgctttgg aaagcaagct atgtggagta tcacgaagat cgtgtaccaa
    13441 acttaccgaa gctgattgtg cttgaaaacg gcactcaaac gttgaagatc cgcattgaaa
    13501 aatgggcgta ttaatgaaga aagtcacctt acccagcccg gcaaaattaa acctgttttt
    13561 atacattacc aataagcggg cagacggtta tcacgagcta caaacgctgt ttcagttttt
    13621 agattttggt gatgaaattg agattgagat cacagaaagt tcagaggttg aattaattaa
    13681 tccggtagaa ggggtggcaa ctgaggagaa tctcatttac cgagcagcaa aacttttgca
    13741 aaaaacgacc gcttgtaggc aaggctgccg tattgggatt aaaaaaaacc taccgatggg
    13801 cggaggtgtt ggtggtggat cttctaatgc tgcaacagta ttagtcggtt taaatcactt
    13861 gtggaatacc gagctttcat tggaaaaatt ggcagaatta ggcttaagtt taggggctga
    13921 tgtgccgata tttgttagag gtatggctgc ttttgccgaa ggcgtggggg aggtgttaac
    13981 tccttgtgat ccacaagaaa aatggtatgt agtgctaaaa ccagaagtgt cgatttcaac
    14041 cgcagcggtg tttaccgacc cgaatttacc tagaaatacg ccaaaacgcc ccttagccga
    14101 attattatct ttaccgtggg aaaacgattg cgaaaaagtt gtacgagaaa agtattctgc
    14161 ggttgaagat cttatacaag aattgttaca atatgctcag tttaggctaa caggcacagg
    14221 tgcttgtatt ttcgccgaat ttgaaacaaa agcagaagcc gagcaggttt ttgcacataa
    14281 accggaagcc gtcttcggtt ttgtggcgaa agggcaaaat atttcgccat tacatcaaat
    14341 gttattccaa atctaaccat cttaacctta aggatcaatc catgacagat attaaactgt
    14401 ttgcgggcaa cgcaactccg gaacttgcta atcgtattgc aaaacatctt tatacctcat
    14461 taggtaatgc aacagttggt cgttttagcg atggtgaaat tcaagtacaa atcaatgaaa
    14521 atgtgcgtgg aggcgatatt tttattgtgc aatccacttg tgcaccaact aatgataatt
    14581 taatggaatt gatcgtgatg gtagatgcgc ttcgccgtgc atcagctggt cgtattaccg
    14641 cagtgattcc gtatttcggt tatgcacgcc aagacagacg cgtgcgttcg gcccgtgtgc
    14701 caattaccgc aaaagtgatt gctgatttct tatccagcgt aggggtagat agagtcttaa
    14761 cctgcgactt acacgcagag caaattcaag gtttctttga tgtgccggta gataacgtat
    14821 ttggttcccc tgtgttaatc gatgatattt tgaaaaaatc cgatttggtc aaccctatcg
    14881 tggtttctcc agatattggt ggggttgttc gtgcaagagc ggtggcaaaa ttattaaatg
    14941 ataccgatat ggcaattatt gataaacgtc gtccaaaagc gaatgtatca caggtaatgc
    15001 acattatcgg tgatgtggct gatcgtgact gtattttagt tgatgatatg atcgatactg
    15061 gtggtacttt agtcaaagcg gctgaagcgc tgaaagaacg tggcgcccgt cgtgtctttg
    15121 cttatgccac acacgcggta ttttcaggca cagcagctca aaatttagcg aatcctgctt
    15181 tagatgaaat tgtagtaact gatactattc cactgagtaa tgagattaaa gcattaaata
    15241 aagtacgtgt tttaacatta tccggtatgt tagcagaagc aattcgccgt attagcaatg
    15301 aagaatccat ttcagcaatg tttgatgcct aatttatgag aaaaaaccca gtcgaaagat
    15361 tgggtttttc atatctgttc actactatat ttagtttaag ataatttgta tgtattccct
    15421 gattcgtaaa ttactttttg cactagatac ggaaaaagca catcaattct ctattcaggc
    15481 acttgcttta gccggaaaat cgcctttttc gattctgcca acacccgata atccgaccca
    15541 agtaatggga ctcacgttta aaaatccaat agggcttgca gccggagcgg ataaaaacgg
    15601 tgaagcgata gatggttttg ggaaattagg atttgggttt attgaggttg ggactattac
    15661 tcctctcgct caagacggaa accctaaacc gaggcaattt cgtattgtag atgcagaggg
    15721 aattattaac cgcaatggat ttaataataa aggcgttgat gttttagtag aaaatgtaaa
    15781 aaatgcaacc tatagtggta ttttaggtat taatattgga aaaaatgcgg ttacaccaat
    15841 tgagcatagc tttgatgact accaaatttg tttgcgtaag gtttaccaat atgctgatta
    15901 tgtggcggtg aatatttcat cacccaatac caaaaatttg cgcagtttac aatatggtga
    15961 ggcattagat gatttgctgc gaaacctcaa aaatgagcag gcacaacttt cgcaaaaaat
    16021 ggggaaatat cgaccgcttg ttttaaagat tgcgcctgat ttaaccgatg aagaaatcgt
    16081 ttctgtagcc gatagtctta ttcgttacca aattgatggg gtgattgccg gcaatactac
    16141 cttgtctcgt gaaagtattg ccggattacc gtattcggaa caactgggtg gattaagtgg
    16201 caagccatta cactcattaa gtacaaggtt aatttcaaaa ttatctgccg agttgggcga
    16261 gagaatacca attataggtt gtgggggagt ccattcgtta gagagtgggc aggagaaagt
    16321 gaatgcaggt gctagtttat tgcaagtcta ttccgcaatg atttaccaag gaccatcatt
    16381 aattcaaacc ttagccaaac accttcagct gaacagttag ggatagattt ctagttaaaa
    16441 atgattggtt aggtttattt ctgtcatctt agtggaatag atggcggtat tagctatcta
    16501 tcaaaaatag acaaaattgc ctatttttgc tatcttgcac tgcaaattta tctacaaaat
    16561 ttaaacaaat tttataaaat tatataaaaa tcgtttaaaa tcaaatagat aataagttt
//
LOCUS       DANLUQ010000046        16303 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.046, whole genome shotgun sequence.
ACCESSION   DANLUQ010000046 DANLUQ010000000
VERSION     DANLUQ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16303
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.046"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            145..474
                     /locus_tag="PXB27_001144"
     CDS             145..474
                     /locus_tag="PXB27_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345944.1"
                     /translation="MKKYLFFAFMTLSNVAYPNLDEWYSASDKERREFIAKHFAEETV
                     RSDRNTPYDYAMCTGRMFAKQLTRDEVKDYPLANTKPQYLTRDRRIEIMSNLPREILQ
                     NCAKYIQ"
     gene            complement(510..1262)
                     /locus_tag="PXB27_001145"
     CDS             complement(510..1262)
                     /locus_tag="PXB27_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDL5345945.1"
                     /translation="MAYKPPFTVTTKMLNLVVEISHKLGTLQLDFERNLHLRKNNRLR
                     SIQASLAIEANSLTIEQVTAIIKGKKVLGHPKDIREVKNAYDAYDEMMHYNPYSIKDF
                     LKAHKFLTADLVNDAGAFRSKDVGIYNFSGQLVHMGARPQFVENLINELFSWAKQDDT
                     PALIKSSVMHYEIEMIHPFSDGNGRMGRLWQNVLLAQWNPLFAWLPIETMVYENQQRY
                     YQMLSLADSQNDSTGFIEFMLEMILETLLKLK"
     gene            complement(1301..2548)
                     /gene="clpX"
                     /locus_tag="PXB27_001146"
     CDS             complement(1301..2548)
                     /gene="clpX"
                     /locus_tag="PXB27_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HDL5345946.1"
                     /translation="MAFEKEPHCSFCGKKRTEVDQLVEGTEGYICNECIEESYALLHG
                     EEEPLIDENQDDEGSFFTNVPTPHELHAHLDDYVIGQEHAKKVLSVAVYNHYKRLRSA
                     LSNHQATNGVELGKSNILLIGPTGSGKTLLAETLARRLNVPFAVADATTLTQAGYVGE
                     DVENVIQKLLMKCDFEAEQAERGIVFIDEIDKITRKSESASITRDVSGEGVQQALLKL
                     LEGTVANINPQGGRKHPKGETIPVDTSKILFICGGAFAGLDKIVEARTNTQGGIGFAA
                     ALKKDKDRQDLTELFKQVEPEDLVKFGLIPELIGRLPVVTPLQELDEEALIQILTEPK
                     NAIIKQYQALFEMEGVELHFTKDALISIAQKAISRKTGARGLRSIVENLLLDTMYDLP
                     TLNAKKVTIGKGCVENGDKPKVE"
     gene            complement(2548..3141)
                     /gene="clpP"
                     /locus_tag="PXB27_001147"
     CDS             complement(2548..3141)
                     /gene="clpP"
                     /locus_tag="PXB27_001147"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDL5345947.1"
                     /translation="MALVPMVVEQTSKGERSYDIYSRLLKERIIFLNGQVEDNMANLI
                     VAQLLFLEAEDPEKDIYLYINSPGGVVTAGLAIYDTMNFIKPDVATLCTGQAASMGAF
                     LLSAGAKGKRFALPHARIMIHQPLGGARGQATDIQIQAEEILRLKATLTRRMAEHSGQ
                     SYEKVLADTERDNFMSAEEAAEYGLIDKVLTSRSEVA"
     gene            3253..3636
                     /locus_tag="PXB27_001148"
     CDS             3253..3636
                     /locus_tag="PXB27_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HDL5345948.1"
                     /translation="MTLTEIYQKFERCKSWEERYRLLIQLSRLLPKPSEQELAQIAEI
                     EGCESRLWFEFQAEPRKVVAYSDARLMQGILFIVSVALTEKTAEELKGFDLKTMFDEL
                     KISQNLTSTRLNGLGQLQKHIMMNL"
     gene            complement(3740..3816)
                     /locus_tag="PXB27_001149"
     tRNA            complement(3740..3816)
                     /locus_tag="PXB27_001149"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3780..3782),aa:Asp,seq:gtc)
     gene            complement(3905..4660)
                     /locus_tag="PXB27_001150"
     CDS             complement(3905..4660)
                     /locus_tag="PXB27_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL5345949.1"
                     /translation="MKKSIYDTPIFFERYQQLRQNPISMNEVVEKPTMFSLLPDLTGK
                     KVLDLGCGTGVHLAHYLTLGAEKVVGLDLSELMLQQAESDLSAHWQKGNDFSLYCLPM
                     ERLNEISEGDFDVVTSSFAFHYIADFSELLAKISQKMTACGTLIFSQEHPIVTCYREG
                     YRWEKNQEKQQVAYRLNYYREEGERDRSWFQQPFKTYHRTMATIVNNLIQGGFEIVQM
                     EEPMLAGQPRWHNEFKDLQHRPPLLFIKAIKQK"
     gene            complement(4720..5715)
                     /gene="trpD"
                     /locus_tag="PXB27_001151"
     CDS             complement(4720..5715)
                     /gene="trpD"
                     /locus_tag="PXB27_001151"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HDL5345950.1"
                     /translation="MQALLDKLFANQALAQAESHRFFSEVIKGNVSNEQLAAALIALK
                     LRGETVDEIAGAAKAALENANPFSTPDYPFADIVGTGGDGHNTINISTTSAIVAATMG
                     YKIAKHGNRSVSSKTGSSDVLSHLGINVDVSPETARLALDETNICFLFAPTYHSGFKY
                     AAPVRQALKTRTIFNILGPLVNPAHAKRQLLGVYSPELVKVYAETVKALGYEHTIVVY
                     GAGLDEVAVHGETQVAEIQNGQIDYYTLTPEDFGIERHSLEDLKGGEPAENAEKVTAI
                     LQGKGKLAHIDAVAANVALLMKTFGEDDLKANVQKVKETLESGKALETLRKLSTY"
     gene            complement(5733..6119)
                     /locus_tag="PXB27_001152"
     CDS             complement(5733..6119)
                     /locus_tag="PXB27_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005543439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HDL5345951.1"
                     /translation="MISVYGLKQTLADRRALIADVIFDCMQMSLGVPKQRHALRFDLL
                     DAENFYPPINRSQDFIVIEINLMQGRSEQLKKRLIKGLFSALHTKVGIVPMDVEITIK
                     EQPEHCWGFRGMTGNEATDLEYQAHR"
     gene            complement(6112..6702)
                     /locus_tag="PXB27_001153"
     CDS             complement(6112..6702)
                     /locus_tag="PXB27_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HDL5345952.1"
                     /translation="MANILFIDNFDSFTYNLVDQFRELGHQVTIFRNDYPLEDFLAKA
                     ETTENCLVALSPGPGNPAEAGNLLEIIRRLKDKVPMIGICLGHQALIEAFGGKVVHTG
                     TVLHGKVSRIEHDNQAMFSGINNPMLVARYHSLMGDELPAEFIVNAKFEHIVMAIRHK
                     TLPICGFQFHPESILTVQGTKLLKQSVEWLLGEKND"
     gene            complement(6712..8268)
                     /gene="trpE"
                     /locus_tag="PXB27_001154"
     CDS             complement(6712..8268)
                     /gene="trpE"
                     /locus_tag="PXB27_001154"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HDL5345953.1"
                     /translation="MPQLTITQTELPYFADTTRVYYALCGERPHTLLLDSAEIQSKNS
                     VKSLLFAKSAVHIFCEDQTVTFHALTPNGEAVLPLIANALSLLNPAPTCKFLDKNDRL
                     QAIFPTISNQLDEDSKLKTTTVFDGLRTVSQLFADSTSPIYLGGLFSYDLVANFIPME
                     NISLQDDGLSCPDYTFYLAEQLVTIDHQTETAVLNTFCFEPNEQTNLNQQAVEFTQKL
                     ANLSHIELPLANQTATSEATANIEDEDFKAIIRKLKHHINIGDVFQIVPSRRFSLPCP
                     NTLASYKQLKINNPSPYMFYMQGENFTLFGASPESALKYSQANRQLEIYPIAGSRPRG
                     FDEQGNIDPELDSRLELELRLDQKELAEHLMLVDLARNDVARVSESGTRRVVDLMQID
                     RYSQIMHLVSRVIGTLRKDLDALHAYQACMNMGTLTGAPKIKAMQLIYQVEQQKRHSY
                     GGAVGYLTSDGNLDTCIVIRSAFVQNGIAYVQAGCGEVLDSDPQLEADETRHKARAVL
                     KAIGQVNAKA"
     gene            8410..9027
                     /locus_tag="PXB27_001155"
     CDS             8410..9027
                     /locus_tag="PXB27_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDL5345954.1"
                     /translation="MTQFFYIHPDNPQQRLINQAVEIIRNGGVIVYPTDSGYALGCAM
                     GDKHAMDRIVAIRKLPENHNFTLVCSDLSELSIYALVNNQAYRLIKNNTPNPYTFILP
                     ATKELPRRLMTKRKTVGIRVPDNAIALALISALGEPILSCSLMLPETEMTESDPDEIR
                     DHLQNRVDLIIHGGYLGQQPTTVVDLTDDVPVIIREGSGDPTSFN"
     gene            9173..10240
                     /gene="rluB"
                     /locus_tag="PXB27_001156"
     CDS             9173..10240
                     /gene="rluB"
                     /locus_tag="PXB27_001156"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HDL5345955.1"
                     /translation="MTTFQKKSTATFRADKRGESRPSFKKEEQKSERKFGKTSEKPPR
                     PTSKPLTQRIAKKVEKTTASEVASQEKKAKVVGEKLQKILARAGQGSRRELEEIIAAG
                     RVSVDGKMATLGDRVQVSSSTKIRIDGHLVNLMPAQKEICRVLMYYKPEGELCTRSDP
                     EGRSTVFDRLPRLSGARWIAVGRLDINTSGLLLFTTDGELANRLMHPSREVEREYSVR
                     VFGNIDDAMLQRLRKGVQLEDGPANFKQIKVVGGTGLNQWFDVTLTEGRNREVRRLWE
                     SQGVEVSRLIRIRYGNIKLDKGLPRGGWEEMGLEQVNYLRELVGLPPETETKVEVGVN
                     RRRTNIRQIRKAVKQHQKYRG"
     gene            10306..12078
                     /locus_tag="PXB27_001157"
     CDS             10306..12078
                     /locus_tag="PXB27_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HDL5345956.1"
                     /translation="MSWDIVATLAVTGIAVFLFATEKMRMDVVAILVLCSLVLLGQVD
                     TKSALSGFSNSATVTVTAMFVLAAGLQNSGALDSVGSLLAKVKSPWLFLLVLCGVNAA
                     VSPFVNNTAVVAVLIPIVIAAAQNIKMAPSKALIPLSFSSQMAGVCTLIGTSTNLLVN
                     AIAQKQGHSGFGMFEFAPLGIIFLVVGVAYLLVTSRFLLPESHLQLEDGEGFGKYVSE
                     LKVNKDSVLIGKSTFESGLNEEFNLFTIGVLRDGERLSTPSHQVLQKHDILLLRGESE
                     NLAKVREKYGLHHVVYGRRENDEDNLDEDLMVAEVMISPISRWIGGTIPILQQRWNKN
                     ATVLGIQRQSKVIRERLRTTAFKMGDILLLTLPKEDMETLRQDKDFIVLSSDLVKTEE
                     SWKSKFALGVMVLVVATAALGWVPISVSALIGAVAMCVAGCLTAEEAYQSIDWKIILV
                     LAGLLPLGEAMANSGAAQFIVDNTLGKVGEFGPLVVLAVLYLLTMVLTEFMSNAGTAV
                     LLTPIAVSTAKMLGVDASPFIIAVMFAAATSFMTPVGYQTNTMVYGAGGYKFTDFIKI
                     GLPLNVIYWILGIVLIPIFFPFNP"
     gene            complement(12168..>16303)
                     /locus_tag="PXB27_001158"
     CDS             complement(12168..>16303)
                     /locus_tag="PXB27_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HDL5345957.1"
                     /translation="IVWNHAKRTWIVASELSRSANKSNATNTSLTTNIIKLSTLSLAL
                     SAGFASAATYSPTYGTIPDNNPQTSIAIGDGSKVNSTLVSTITGLEGNSGKVSTALGY
                     NTTATADFSTALGGFANASFASTAVGGQATADKRSVAVGYNATAMGLREVFIGDHAGL
                     NHSNSTEYNIGIGYSASSNVTGNNTISIGNTAGDGTSGSHNIAIGTYANAKLAGPTTN
                     VTSDDNIAIGNSALANGVNNYKTTAATGETVIGKATAVGSHANATGIVSSAYGAEANA
                     TAIHSTAIGAKSQANGDNSTAIGYEAKTSGHGATSLGYYANATANLTTAVGTNAGATS
                     DYASAFGREANASGGSATALGNRATASGAASVALGVSAKATNQRTIAIGESSNASAFN
                     ATAIGRNATAEHTDSIALGSNSVTAIAIPTTNATVNGITYSDFAGTNPIATVSIGAEG
                     KERTITNVAAGRISLSSTDTINGSQLYLTQQAIGNVAATTANILGGGAAVTENGNITF
                     PTYALVNGTPDADKEGKQGRYTTVSAALSALNTAVISPLTFAGDTGTNFERHLGSTVK
                     IKGGSTGILTENNIGVVADGNSTLTIKLAEKVNLGANGSLTTGDTVVNNTGITIANGV
                     ADKPVSLTKSGLDNGGNKIANVAAGDVDTDAVNVSQLKQAISKFATHYVSISDDGIQR
                     ANYDNSGSSGVNPMAIGVATSANGELATALGSEAEANGERTTAVGPRATADGMNATSI
                     GYNANANATNALAVDSAANANADTSTAIGTASTATATRATALGSKSEATGENSTAVGY
                     EASSIGADSLAAGYNANASGTQSTALGNSANAGGIWSTSVGRNANAAGSSAIALGNSA
                     NAAGVASIALGVSSQATTTAAVALGQNAKATHQGSVALGTNSETVATVATKSATLNGN
                     TYTFAGTTPSSTVSIGSVGNERTLTNVAAGRILDSSTDAINGSQLYAAYTEIDGLNTK
                     VNELSNGALTFVDDAGTEIVRKLGTSLNVKGGADATILTDNNIGVVATDANTLTVKLA
                     KDIDLTPAGSVAVGNSKLNNNGLTINNGPSVTMTGVDAGKLKITNVADGDISPISADA
                     VNGSQLYDTANTIATALGGNSSVNANGAVSAPSYTVVDGAPTNEVSKTVNNVGSAITA
                     LNDAVTSPLTFAGDTGTPSQRKLGSTVTVKGGVSNESQLTDNNIGVISNGNGSLTVKL
                     AKDIKVNSVTAQTVTANIAVADTVKTGDTTIDTNGLTIVGGPSITKTGINAAGTKVTN
                     VKAGTEDTDAVNFSQLKATEKNINNKINNIDSKVNKVDKRLRAGIAGATATAGLPQAY
                     LPGKSMLATAGDTYRNEAAIAVGYSRISDNGKVIYKLTGNSNTRGDFGGSIGMGYQW"
BASE COUNT         4481 a         3309 c         3491 g         5022 t
ORIGIN      
        1 ttagcgtcag ctgcatcagt aattttgcct ctttatgcag catataaaga ctatcaattt
       61 ggggagaata tgttatctct tatggatata attattttcc cgttaggtat tgttcgtgga
      121 cttatgtatt tatttggata gattatgaaa aaatatttat tttttgcttt tatgacatta
      181 agtaatgttg cttatcctaa tttagatgag tggtactcag cttcagataa agagcgaaga
      241 gaatttatcg caaaacattt tgcagaagaa acagtaagaa gtgatagaaa tacaccgtat
      301 gattatgcta tgtgcactgg tagaatgttt gctaaacaac tgactcgaga tgaagtcaaa
      361 gactaccctt tggcaaacac taagccgcaa tatttaaccc gtgatagacg gattgagatt
      421 atgtctaatt tacctcgtga aatattacaa aattgtgcta aatacataca gtaattttat
      481 gagtaacacc actttggaat taagtggtgt tattttaatt ttaataatgt ctctaaaatc
      541 atctccaaca taaactcaat aaagccggta gaatcatttt ggctgtctgc gagagaaagc
      601 atttgataat aacgttgttg attttcgtaa accattgttt caatcggcaa ccaagcgaaa
      661 agtgggttcc attgtgctaa aaggacattt tgccataatc gacccattct gccgtttcca
      721 tcactaaagg ggtgaatcat ttctatttca tagtgcataa ccgagctttt aatgagtgca
      781 ggtgtatcat cttgtttagc ccaagagaac aactcattaa tcaaattctc tacgaattga
      841 ggacgagccc ccatatgcac aagttgccca ctgaaattat aaatgccgac atcttttgaa
      901 cgaaaagccc ctgcgtcatt tactaagtca gcagtaagaa acttatgtgc tttgagaaaa
      961 tctttaatcg agtaaggatt atagtgcatc atttcatcgt aagcgtcata ggcatttttg
     1021 acttcacgaa tatctttggg gtgtccgaga acctttttgc ccttaatgat tgcggtaact
     1081 tgctcaatcg ttaatgaatt tgcctcaata gctaaagagg cttgaattga tctcaagcgg
     1141 ttattttttc taagatgtaa attacgctca aaatccaatt gtagcgttcc caatttatga
     1201 gaaatttcta caaccagatt cagcatttta gtggtaactg taaaaggtgg tttataagcc
     1261 attttgcaat aatcctaaaa aattaaaccg cttgtcattg ttactccacc ttcggcttat
     1321 cgccgttttc aacacagcct ttgccgatgg tgactttttt cgcattgagg gttgggagat
     1381 cgtacattgt gtcaagtaat aagttctcga caatagaacg taacccacga gccccggttt
     1441 tgcggctaat cgctttttgg gcaattgaaa ttaatgcatc tttggtgaag tgcaattcaa
     1501 cgccttccat ttcgaataag gcttgatatt gcttgatgat cgcatttttc ggctcggtta
     1561 agatttgaat cagagcttct tcgtccaatt cttgcagcgg agtcaccacc ggtaaacgcc
     1621 cgattaattc aggaattaag ccgaatttga ctaaatcttc cggttcaact tgtttgaata
     1681 gctcggttaa atcttggcga tctttatctt ttttcaatgc cgctgcaaaa ccgataccac
     1741 cttgagtgtt ggtacgggct tccacgattt tatctaaacc ggcaaaagca ccgccgcaaa
     1801 taaagagaat tttggaggtg tccaccggaa tggtttcgcc tttcgggtgt ttgcgaccgc
     1861 cttgtgggtt aatgttcgcc accgtgcctt caagtagttt taataaggct tgttgaacac
     1921 cttcgcccga tacatcacgg gtaatggatg cactttccga tttacgggtg attttgtcga
     1981 tttcatcaat aaaaacaatg cctcgttccg cttgttcggc ttcaaaatcg catttcatta
     2041 ataatttttg aatcacgttt tcgacatctt ctcccacata gcctgcttgg gtaagggtag
     2101 tagcatcagc taccgcaaaa ggtacgttta aacggcgggc aagggtttct gccaacaagg
     2161 ttttaccgct accggtcggc ccgattaata aaatattact tttacctaac tctacgccat
     2221 tagtggcttg atggtttgac aatgcacttc ttaagcgttt gtagtggtta taaacggcca
     2281 ccgaaagcac ttttttcgcg tgttcttgac cgatgacata atcatctaaa tgagcgtgta
     2341 attcgtgagg tgtcggcaca ttggtgaaaa aactgccttc atcgtcttgg ttttcatcaa
     2401 ttaacggctc ttcttcaccg tgtaataagg cgtaggattc ctcgatacat tcattacaaa
     2461 tatagccttc cgtgccttcg actaattgat cgacttcggt acgtttttta ccgcaaaaac
     2521 tacagtgggg ttctttttca aatgccatta cgccacctca ctgcgagaag ttaatacttt
     2581 gtcgattaag ccatactcag cggcttcttc cgcagacata aaattatcac gctcggtatc
     2641 ggctaacact ttttcatagc tctgaccgct gtgttctgcc atacggcggg taagggtagc
     2701 tttcaagcgt aaaatttctt ccgcttgaat ttgaatatcg gttgcttgac ctctcgctcc
     2761 acctaaaggt tggtgaatca taatacgagc gtgcggcaga gcaaagcgtt taccttttgc
     2821 cccggctgaa agcaaaaatg cccccattga agccgcttgc cctgtgcaaa gggttgctac
     2881 atcgggtttg atgaagttca ttgtatcgta aattgccaag cctgctgtta ccacgccacc
     2941 cggtgagtta atgtataaat agatgtcttt ttccggatct tcggcttcta aaaacagaag
     3001 ttgtgccaca atcaggtttg ccatattgtc ttccacctga ccgtttaaaa aaataatacg
     3061 ttcttttaat aaacgtgaat aaatgtcgta agaacgttcg ccttttgagg tctgttccac
     3121 caccattggg actaatgcca ttttgttttc ctctgtcgta atacaaaatc tcgctattct
     3181 agcaaatttt cagaaaatca ttaacaacaa gcggtcagat tgttaaaatc ttttgcaaat
     3241 gatgaggaaa ttatgacatt aacagaaatt taccaaaaat ttgaacgttg taaatcgtgg
     3301 gaagaaagat accgcttgtt aattcagcta agtcgcttgt taccaaaacc aagtgagcaa
     3361 gagttggcac aaatagcgga aattgagggc tgtgaaagcc gtttatggtt tgaatttcaa
     3421 gctgagccac gaaaagtagt ggcatatagc gatgcccgtt taatgcaggg gattttattt
     3481 attgtgagtg tagctttaac tgaaaaaacg gcagaagagc tgaaaggctt tgacttaaaa
     3541 acgatgtttg atgagctaaa aatttcacaa aatttaacca gcacaaggtt aaatgggttg
     3601 ggtcagttac agaaacatat aatgatgaat ttgtaattca ccctcgcccc ttgtgggaga
     3661 gggtgaataa aatccgcagg attttattcg ggagagggga atataaaaaa cctctgattt
     3721 ctcagaggct taaatttgtt ggcggaacgg acgggactcg aacccgcgac cccctgcgtg
     3781 acaggcaggt attctaacca gctgaactac cgctccatac tggtgctaaa ataatagtgg
     3841 tttcctcaac attgagcaaa tagtttttgc aaaatagtgt ttaattggtt gtaaaataac
     3901 cactttattt ctgcttgatc gccttgataa acagcaacgg cggacggtgc tgtaaatctt
     3961 taaattcatt gtgccaccga ggttggcctg ccagcattgg ttcttccatt tgcacaatct
     4021 caaacccgcc ttgaataagg ttattcacaa tggttgccat tgtgcggtgg taggttttaa
     4081 agggttgttg aaaccaactt ctgtcccgtt cgccctcttc ccgataatag ttcaagcggt
     4141 aagcaacttg ttgtttttct tggtttttct cccaacgata gccttctcgg taacaggtga
     4201 caatcgggtg ttcttgcgag aaaatcagcg tgccacaagc agtcattttt tgcgaaattt
     4261 ttgcaagtag ttcggaaaaa tcagcaatat aatgaaacgc aaaagagctg gttactacat
     4321 cgaaatcgcc ctcgctgatc tcatttaatc gctccatcgg caggcagtaa agggaaaaat
     4381 cgttgccttt ttgccaatga gcagaaagat cgctttctgc ctgttgtagc attaattcag
     4441 ataaatccaa ccccaccact ttttctgcac cgagcgttaa ataatgggct aaatgaactc
     4501 ctgtgccaca acctaaatcc agcacttttt tgccggttaa gtctggcagt agagagaaca
     4561 ttgttggctt ttccaccact tcattcatac ttattggatt ttgtcgaagt tgttggtaac
     4621 gctcaaagaa aatcggagtg tcgtaaatgc tttttttcac ttaatagcct ttaaacaaat
     4681 ataaaataag gcgggttaaa acccgccact acgatatgat tagtaagtcg ataattttcg
     4741 caaagtttct aacgcttttc cactctcaag cgtttctttt actttttgca cattggcttt
     4801 taaatcatct tctccaaagg ttttcattaa taatgcgaca tttgctgcca ccgcatcaat
     4861 atgagccagt ttaccttttc cttgtaaaat agcggttact ttttcggcat tttctgcagg
     4921 ttcaccgcct ttgagatctt ctaacgaatg gcgttcaatg ccgaaatctt ccggcgttaa
     4981 ggtgtaatag tcgatctgtc cgttttgaat ttccgcaacc tgcgtttcgc cgtgaacggc
     5041 aacttcatct aaacctgcac cgtataccac aatagtgtgt tcgtaaccta aggcttttac
     5101 ggtttcagca tagactttca ctaactcagg ggagtacaca ccgagtaatt ggcgtttagc
     5161 gtgagccggg tttaccaacg gccctaaaat attgaaaatc gtgcgggttt ttaaggcttg
     5221 gcgaacaggg gcggcatatt tgaatccgct gtggtaggtt ggggcaaaca aaaagcagat
     5281 gttggtttca tctaaagcaa ggcgtgccgt ttcaggtgaa acgtcaacat taatacctaa
     5341 atggcttaat acatcgcttg agccggtttt acttgatacg ctacggttgc cgtgtttggc
     5401 aattttatag cccattgtgg cggcaacaat cgcacttgtg gtagaaatgt tgatggtatt
     5461 atgcccatcg ccacctgtgc cgacaatatc cgcaaaaggg taatcaggcg ttgagaacgg
     5521 attggcattt tcaagtgcgg cttttgcagc acctgcaatt tcatcaacgg tttcgccacg
     5581 cagttttaga gcgattaagg cagcagcaag ttgttcattt gatacattgc ctttaataac
     5641 ttcactaaaa aaacggtgag attccgcttg ggctaaggct tggttggcga ataatttatc
     5701 taaaagtgct tgcatagtct cttccttttt aattagcgat gtgcctgata ttccaaatcg
     5761 gttgcctcgt tgccggtcat tccacgaaag ccccagcaat gctctggctg ttctttaatg
     5821 gtgatttcaa cgtccattgg cacaatgccg actttggtgt gtaaggcact aaatagtcct
     5881 ttaattaagc gttttttgag ctgttcgctc cgcccttgca tcaggtttat ttcaatcaca
     5941 ataaaatctt ggctacggtt aattggggga tagaaatttt ccgcatccaa taagtcaaaa
     6001 cgcaaagcgt ggcgttgttt tggtacgcca aggctcattt gcatacagtc aaaaatcacg
     6061 tctgcaatca gggctctgcg gtctgctaag gtttgtttta atccatatac actaatcatt
     6121 tttttctcct aataaccatt ccaccgactg cttaagtaat tttgtgcctt gcacggttaa
     6181 aatggattcc gggtggaact ggaaaccgca aatcggtaag gttttgtggc gaattgccat
     6241 cacaatatgt tcaaatttcg cattaacgat aaattctgcc ggtaattcat cgcccattaa
     6301 tgagtgataa cgagcgacaa gcattggatt gttaatgcct gaaaacatcg cttggttatc
     6361 gtgttcaata cgagaaactt tgccgtgcaa tactgtgccg gtatgcacta ctttgccgcc
     6421 aaaggcctca attagagctt gatgtcctaa acaaatgcca atcatcggta ctttgtcttt
     6481 cagacggcga ataatttcca ataaattgcc tgcctcagcc ggattacccg ggccggggga
     6541 aagggcaacc aaacaatttt cggtggtttc agctttggct aaaaaatcct caagcggata
     6601 atcattgcgg aaaatcgtta cctgatgccc taattcacga aattgatcga ccaagttata
     6661 ggtaaaagag tcgaaattat caataaaaag aatatttgcc atagtttgtc cttatgcttt
     6721 tgcattcacc tgcccaatcg cttttaacac cgcgcgtgct ttatggcgtg tttcatctgc
     6781 ctctaattgt ggatcggaat ccagcacctc accacaacca gcttgcacat aggcaatgcc
     6841 attttgaaca aaggcggaac ggatcacgat acaggtatct aaattgccgt ctgaggttaa
     6901 atagcccacc gcaccgccgt agctatggcg tttttgttgc tcgacttggt agatcagttg
     6961 cattgcctta attttcggag caccagttag cgttcccata ttcatacaag cttgataagc
     7021 gtgcagagcg tcaagatctt ttctaagtgt gccgatcaca cgagaaacta agtgcataat
     7081 ttgcgaatag cggtcaattt gcattaaatc aaccacacgg cgagtgccgg attcgctcac
     7141 acgagccaca tcattgcggg caagatcgac taacattaaa tgttcggcaa gctctttttg
     7201 gtctaaacgc agttctaatt ccaaacggga atctaattca ggatcgatat taccttgttc
     7261 atcaaaaccg cgtgggcgag agccggcaat cgggtaaatt tccaactggc ggttggcttg
     7321 cgaatatttc aacgcacttt caggcgacgc cccaaacaga gtgaaatttt cgccctgcat
     7381 atagaacata tacgggcttg gattgttgat tttgagctgt ttatagctgg caagagtatt
     7441 cgggcaaggc aagctgaaac ggcgagaggg cacaatttgg aacacatcac cgatattaat
     7501 atggtgtttg agtttgcgga taatcgcttt gaaatcttca tcttcaatat tggcggttgc
     7561 ctcactcgtt gcggtttggt tcgctagagg cagctcaata tgtgacagat ttgcaagttt
     7621 ttgcgtaaat tcgaccgctt gttggttcaa attggtttgt tcattcggtt caaaacagaa
     7681 ggtgtttagc accgcagttt cggtttggtg gtcgattgta accagttgct ctgctaaata
     7741 gaaagtgtaa tctgggcaac ttaagccatc atcttgcagg ctgatatttt ccatcggaat
     7801 aaaatttgcc accaaatcgt agctaaataa tccgcccaaa tagataggcg atgtggaatc
     7861 agcaaacagt tggctcaccg ttcttagccc gtcaaacacg gtggtggttt tgagcttgct
     7921 gtcttcatct aactggttgc tgatggttgg gaaaatcgct tgcaagcggt cgtttttgtc
     7981 taaaaatttg caagtcggag cagggtttaa caggctcaat gcgtttgcaa tcaaaggtaa
     8041 aaccgcttct ccgtttgggg ttaaagcgtg gaaagtcacg gtttgatctt cacagaaaat
     8101 atgcactgcc gatttcgcaa acagcaaact ttttacactg tttttacttt gaatttccgc
     8161 tgaatctaat aatagcgtat gtgggcgttc gccgcataat gcgtaataaa cacgggtcgt
     8221 atcggcaaaa tagggtaatt ctgtttgagt aatcgttaat tgaggcatag caaattccta
     8281 ttcaattttt cgtactatta aactagtaca ttaaaaagaa gtcaagcaat tattccagat
     8341 aattatgcga gcttgggcga acttggtgta taattagccc aatttttaga aagtcgaagg
     8401 cttgagacta tgacacaatt tttttatatc caccccgaca acccacagca acgcttaatt
     8461 aaccaagcgg tagagattat tcgaaacggt ggcgtaattg tttaccctac cgattcaggc
     8521 tatgctctcg gttgtgcaat gggcgataag cacgcaatgg atagaattgt agcaattcgt
     8581 aaattgccgg aaaatcacaa ttttacctta gtttgtagtg atttatccga gctttctatc
     8641 tacgcgttgg tcaataatca agcctatcgc ttaattaaga ataatacccc gaatccttat
     8701 acttttattc tgccggcaac taaggaactt cctcgcaggc tgatgacaaa gcgtaaaaca
     8761 gtggggattc gtgtgccgga taatgcgatc gcattggcgt taatttccgc cttaggtgag
     8821 ccgattcttt cttgctcgtt aatgttgccg gagacggaaa tgaccgagtc agaccctgat
     8881 gaaattcggg atcatttgca aaatcgggtg gatttaatta tccacggtgg ttatttaggg
     8941 cagcagccta caacggtagt tgacttaacc gatgacgtgc cggttattat tcgagaagga
     9001 tcgggcgatc caacatcgtt taattaaatt gatgttgtat gattaatata tagaattatg
     9061 cgtagggggg ctgtgccagt ccgtattaat atcgttattt ggacaggcac aggacctgtc
     9121 cctacaatga aaatcaaatt cagacgcttg tgaaagcgac aaaaggaacc ttatgacaac
     9181 ttttcaaaaa aaatcaactg cgactttccg tgcagacaaa cgtggtgaaa gccgtccctc
     9241 atttaaaaaa gaagagcaaa aatctgaacg caaatttggt aaaacgtcag aaaaaccacc
     9301 acgtccaact tctaagccat taacgcaaag aattgcaaaa aaagtggaaa aaacgaccgc
     9361 tagtgaagtg gcatctcaag agaaaaaagc caaagtagta ggtgaaaagc tacaaaaaat
     9421 cttagcgcga gcagggcaag gttctcgccg tgaattagaa gagattatcg ctgctggacg
     9481 agtgagcgtg gacggtaaaa tggctactct tggtgatcgt gtgcaggtta gctcatcgac
     9541 taaaatccgc attgacggac atttagttaa tttaatgccg gcacaaaaag agatctgccg
     9601 tgtattaatg tattacaaac cggaagggga actttgcacc cgttccgatc cggaaggacg
     9661 atccaccgta tttgaccgct tgcctcgttt aagtggcgct cgttggattg cggtcgggcg
     9721 gttagatatt aacacctcag gcttattatt gttcactacc gatggcgaat tagccaaccg
     9781 tttaatgcac ccaagccgtg aagtcgaacg tgaatattcg gtgcgtgtgt tcggcaacat
     9841 tgacgatgcg atgttacaac gcttacgcaa aggcgttcag ttggaagacg gaccggcaaa
     9901 ctttaaacaa attaaggttg tgggtggcac aggcttgaac caatggtttg atgtgacttt
     9961 aaccgaaggg cgtaaccgtg aggttcgccg cctgtgggaa tcgcaagggg tagaagtcag
    10021 ccgtttgatt cgtatccgtt acggcaatat caaattagat aaaggcttac ctcgtggcgg
    10081 ctgggaagaa atgggcttag aacaagtcaa ttacttgcgt gagctggtag ggttaccgcc
    10141 ggagactgaa actaaagtgg aagtgggggt aaaccgccgc cgaaccaata tccgtcaaat
    10201 tcgtaaagcg gtaaaacaac atcaaaaata tcgtggctaa acaccatctt tggggctgca
    10261 ttagttctgc taatgcagct attttctttc aaaaaaggag catttatgtc ttgggatatt
    10321 gttgcaacat tggcggtcac tgggattgcc gtatttttat ttgcaactga aaaaatgcga
    10381 atggacgtag ttgcgatttt agtgctgtgt tcattagtat tattagggca ggttgatacc
    10441 aaatctgctc tgagtggatt ttcaaactca gctactgtaa ccgtaactgc aatgtttgta
    10501 ttagctgctg gtttacaaaa tagtggtgct ttagatagtg taggcagttt acttgctaag
    10561 gtaaaatctc cttggttatt cttgcttgta ctctgtggcg taaatgctgc tgtttctcct
    10621 tttgtgaata atacggcagt ggtcgcagta ttaattccga ttgtgattgc cgcagcacag
    10681 aatataaaaa tggctccttc caaagccctt attccgttat cattttcttc tcaaatggca
    10741 ggtgtttgta cactgatcgg cacttccact aatctattag ttaatgcaat cgctcaaaag
    10801 caagggcatt ccggtttcgg tatgtttgaa tttgcccctt tggggattat ttttttagtg
    10861 gttggggttg cctatttatt agtgaccagc cgtttcttat tacccgaatc acatttgcaa
    10921 cttgaagatg gtgaggggtt tggaaaatat gtttctgaac ttaaagtaaa taaagactct
    10981 gtattaattg gcaaaagtac atttgaatca ggattaaatg aagaatttaa tctatttact
    11041 attggtgttt tgcgtgatgg ggaaagatta tcgacaccga gtcatcaagt attgcaaaaa
    11101 catgatattt tattgttgcg gggtgaatcg gaaaatttag caaaagtacg agaaaaatat
    11161 ggattgcacc acgttgtgta tggacgccgt gagaatgatg aagataatct ggatgaagac
    11221 ttgatggtgg ctgaagtgat gatttctcct atttctcgtt ggataggagg gactattcct
    11281 attttacaac agcgttggaa taaaaatgca acggtgttag gcattcaacg ccaaagtaaa
    11341 gtaattcgtg agcgtttacg caccactgct tttaaaatgg gggatatttt attactcaca
    11401 ttaccaaaag aggatatgga gacgttacgc caagataaag actttattgt gttatcttct
    11461 gatttagtca aaacggagga atcgtggaaa agtaaatttg ctttaggggt aatggttttg
    11521 gtggtggcaa cagcagcctt aggctgggtt cctatttcag tgagtgcctt aattggtgca
    11581 gttgcgatgt gtgttgctgg ttgtttaacg gctgaagaag cctatcaatc tattgactgg
    11641 aaaatcattt tagtcttagc tggattactg ccattaggtg aagcgatggc aaatagtggg
    11701 gcggcgcaat ttattgttga taacacgctt ggtaaagtag gggagttcgg gcctttagtg
    11761 gtgttagctg tattatattt actgacaatg gtgttaaccg aatttatgag taatgcaggg
    11821 actgccgtgt tgcttacgcc tattgcggtt tctaccgcta aaatgttagg ggtagatgct
    11881 tccccattca ttattgccgt aatgtttgcg gctgcgacca gctttatgac ccctgttggc
    11941 tatcaaacca acacgatggt atacggtgca ggtggctata aattcacaga ttttattaaa
    12001 attggtttac cgctaaatgt aatctattgg attttaggga tagtattaat tccgattttc
    12061 ttccctttta atccataatt gatagataat tatagaaaaa aggtttacag atcagaaact
    12121 ctgtaaacct tttttattca gcaataaatc aattttctat tggaaaatta ccattgataa
    12181 cccatgccaa tactaccacc aaagtcgcct ctggtattgc tgttaccagt taatttatag
    12241 ataaccttcc cgttatctga aatccgagaa taacctactg cgattgcagc ttcattacgg
    12301 tatgtatctc cagccgttgc taacatactt ttacctggta gatatgcttg cggtaaacct
    12361 gcggttgcag ttgcaccagc aataccagcg cgtaaacgtt tatcaacttt gtttactttt
    12421 gaatcaatat tattgatctt attattgata tttttttctg ttgcttttaa ttggctaaag
    12481 ttaactgcat cagtatcttc agttcctgct tttacgttag tcacttttgt tcctgctgca
    12541 ttaatacctg ttttagttat actcggtccc cctacaatag ttaatccatt agtatcaatg
    12601 gttgtatcac cagtcttaac agtatcggct acagcaatgt tagcggttac tgtttgagct
    12661 gtgacagaat taactttaat atctttagct aatttaaccg ttaatgaacc attaccattt
    12721 gaaataaccc cgatattatt atcagttagt tgtgattcgt tagatacccc tccttttact
    12781 gtaacagtag aacctaattt acgttgagat ggcgtgccgg tatcacctgc aaatgttagt
    12841 gggctggtta ctgcatcatt taacgcagta attgcagagc ctacattatt gaccgtttta
    12901 ctaacttcat ttgtaggagc gccatcaacc acagtatagc ttggggctga aacagcacca
    12961 tttgcattta cacttgaatt accacctaaa gcggtagcaa tagtatttgc cgtatcgtaa
    13021 agttggctac cattaactgc atctgctgag ataggtgaaa tatcaccatc agctacatta
    13081 gttattttta atttgccagc atcgacacct gtcattgtga cacttgggcc attattaatg
    13141 gttaagccgt tgttatttag ttttgaatta ccaacagcta cggagccggc aggagttaaa
    13201 tcaatgtctt tcgctaattt aactgttaat gtgttagcat ctgttgccac tacaccgata
    13261 ttattatcag tcaatatggt tgcatcggcc ccacctttaa cattcagaga agtacctaat
    13321 ttacgaacaa tttcagtacc agcatcatct acaaaagtaa gtgctccatt acttaactca
    13381 ttcactttcg tgtttaaacc atcaatttcg gtataagcag cataaagttg tgaaccgttg
    13441 atagcatccg ttgatgagtc caaaatacga cctgctgcaa cattcgttaa agtacgttca
    13501 ttgcctacag aaccaatact tacagttgag cttggcgtag tacctgcaaa tgtgtatgta
    13561 ttgccattta gcgttgcact ttttgttgca actgtggcta ctgtttcaga gttagtacct
    13621 aatgcaacag aaccttggtg tgttgctttt gcattttggc caagagccac tgcagcagtt
    13681 gttgttgctt gtgatgaaac acctaatgca atagaagcta caccggctgc atttgcacta
    13741 ttacctaaag caatcgcaga gctacctgca gcattggcat ttctacccac agaagttgac
    13801 catattccac ctgcattagc agagttacct aaagcggttg actgtgtacc cgaagcattt
    13861 gcgttatagc ctgcagcaag agaatctgca ccgattgaac ttgcttcata acctacagcg
    13921 gttgaatttt caccggtagc ttcagattta gagcctaatg cagttgcacg ggtagctgtc
    13981 gctgttgaag ctgtaccaat agctgttgag gtatcagcat ttgcattggc tgcagaatca
    14041 accgcgaggg catttgttgc atttgcattc gcattgtagc ctatagaagt ggcattcata
    14101 ccatcagctg ttgcgcgagg accaactgca gttgtacgtt caccgtttgc ttctgcttca
    14161 gagcctaatg cggttgccag ctcaccatta gcacttgttg caacgccgat agccattgga
    14221 ttaacaccgc tagagcctga attatcatag ttagcacgtt gaatgccatc atctgaaata
    14281 ctaacataat gagttgcaaa tttgctgata gcttgtttga gttgactgac attgaccgca
    14341 tcagtatcta catcgcctgc ggcaacattg gcgattttat taccgccatt atctaagcca
    14401 gatttagtaa gacttaccgg tttgtctgca acgccgttag cgatggtgat acctgtattg
    14461 ttaacaacgg tatcacctgt tgttaatgaa ccattagcgc ctaagttaac tttttctgct
    14521 aattttatgg ttaaagtact attgccatca gctacaacac caatattatt ctcggttaat
    14581 atgcctgtag atccgccttt gatcttgact gttgaaccta gatggcgctc aaaattagtt
    14641 cctgtatcac cggcaaaggt taacggactg atcaccgctg tgtttaatgc gcttagtgct
    14701 gcagaaacag tagtgtatct cccttgtttg ccttctttat ctgcatctgg tgtgccattc
    14761 actaaagcgt aggttgggaa agtgatattt ccattttcag taacagctgc cccaccacct
    14821 aagatatttg cggttgttgc agcaacatta cctatagctt gttgtgttaa atataattgt
    14881 gaaccgttta tggtatctgt agagcttaaa gatatacgtc ctgcagcaac attggtgata
    14941 gttctctctt tgccttccgc cccaatactt actgtagcaa ttggatttgt tccagcaaag
    15001 tcactatatg taataccatt tacagttgca tttgtagttg gtatggctat agctgtaact
    15061 gagttgctac ctagcgctat cgaatcggta tgttcagcag tggcatttcg acctatagcg
    15121 gttgcattaa aggcggaagc atttgatgat tctccgattg caattgtgcg ttgatttgtt
    15181 gctttagcgc ttacccctag tgcaacagaa gccgcgcctg atgcagtagc acgattgcct
    15241 aaagcggttg cagaacctcc acttgcattg gcttcacgac caaatgcaga agcataatcg
    15301 ctggttgcac ccgcattagt accaacagca gtagttaaat ttgctgttgc atttgcataa
    15361 taacctaatg aggttgcacc gtggcctgaa gtcttcgctt cataaccaat tgctgttgag
    15421 ttatcgccat ttgcttgaga ttttgcacca attgctgtgg agtgaatagc tgttgcgtta
    15481 gcttcagcac cataggcgga gctaacaata ccagttgcat tggcatgact acctacagca
    15541 gttgcttttc ctattaccgt ttctcctgtt gctgctgtag ttttgtaatt attaacacca
    15601 tttgcaagag cggaattacc gatggcgata ttatcatcac tggttacatt tgtagtaggt
    15661 cctgctagtt ttgcattagc ataagtacca attgcaatat tgtggctacc actagtaccg
    15721 tctccagcag tattaccaat agaaatcgta ttgtttcctg ttacattaga gctagccgaa
    15781 taaccaatac caatattgta ttctgtgctg ttagagtgat ttaatccggc atgatcgcca
    15841 ataaaaactt cacgtaagcc cattgcggtt gcattgtacc ctactgctac ggaacgttta
    15901 tcggcagtgg cttgcccacc aacagctgtt gaagcaaaac tagcgttagc aaagcctcct
    15961 aatgcagttg aaaaatcggc tgtcgcggtt gtgttatacc ctaatgctgt agaaacttta
    16021 ccgctatttc cttctaagcc tgttattgtg cttactagtg ttgagttaac tttagatcca
    16081 tcaccaattg cgatagatgt ttgtgggtta ttatctggta tggtcccata ggttggtgaa
    16141 taggttgcag cacttgcaaa acctgcagaa agcgcaagac ttagtgtgct gagtttaata
    16201 atattagtgg ttagtgatgt atttgttgca ttagatttat tagcacttct tgataattca
    16261 gaagcaacaa tccaagttcg ttttgcatgg ttccaaacaa tgc
//
LOCUS       DANLUQ010000047        16064 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.047, whole genome shotgun sequence.
ACCESSION   DANLUQ010000047 DANLUQ010000000
VERSION     DANLUQ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 16064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16064
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.047"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            40..2262
                     /gene="glgB"
                     /locus_tag="PXB27_001159"
     CDS             40..2262
                     /gene="glgB"
                     /locus_tag="PXB27_001159"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HDL5345958.1"
                     /translation="MQDYTYSEKDAFFVERLSSGSCNDPFSYLGIHPSNNGVIIRVFL
                     PSAIACQVIDLNGKPVSSMLKIDDRGFFIAELIGKKTDFSYRLLVEYPDSTVDIEDPY
                     RFKTHIQELDNWLFAEGKQLRPYEKLGAHLVTQNEVGGVHFSVWAPNAQRVSVVGDFN
                     HWNGLVHSMRFHPDSGIWDIFLPNVKKGECYKFEVLDRNGVLRLKSDPYAFASQFRPE
                     TASIVSGLPSIVPAEKTCRNANAPDKPISIYEVHLGSWRRNIENNYWLSYEELADELI
                     PYVKDMGFTHIELLPISEFPFDGSWGYQTTGIYSPTSRFGTPEGLSYFIQKAHDKGIG
                     VILDWVVGHFPVDEHGLVYFDGTHLYEHSDPKEGYHKDWNTLIFSYGRNEVSNYLSGN
                     ALYWIERFGIDALRVDAVASMIYRDYSRSDGEWIPNKYGGRENLEAIDFLRHTNNILR
                     DESQGGASIAEESTSFAGVSHSTERGGLGFNYKWNMGWMNDTLRYMQKDPIYRRYHHN
                     LMTFGMTYQYSEQFVLPLSHDEVVHGKGSLLNKMPGDCWQKFANLRAYYGYMWGYPGK
                     KLLFMGNEFAQGKEWNFAESLDWYLLGEEGGEWHKGVLGWVKALNHLYTQTPALFEQD
                     YNPEGFEWLVVDDHDNSVFAFERKAKNGDSVIVVSNFTPIVRENYRFGVNELAEYQEI
                     LNSDLACYCGSNVSNANRFASELIASHGKAYSLSITLPPLSTIFLKRAADKKTKQNKT
                     "
     gene            2286..4298
                     /gene="glgX"
                     /locus_tag="PXB27_001160"
     CDS             2286..4298
                     /gene="glgX"
                     /locus_tag="PXB27_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HDL5345959.1"
                     /translation="MHYFRGKPFPLGNKLDRQAGEFGTNFAIVSQNATAIELCLFDEN
                     HHETRIPMFSSGDTWHIFVAGVQAGTKYGYRVKGIQDEQAGLLFNPQKLLTDPYAKAI
                     EGKPDLSSAEAAAWYKWNDERDNASLAPKSIVIDEHFDWENDSQLFTPWAETIIYEMQ
                     VKGFSQRNFQIPSDIRGTFAGLAHPVSIDYLKTLGITAVELLPVTYHIDETHLQKQGL
                     VNYWGYNVLGNFAIDPNLAADKQNPLKEFKQLVKTLHQAHIEVIMDIVFNHTAESDKN
                     GPMLSFRGIDNTAYWLDKQGEYENWTGCGNTLNLSNPYILCWAIDCLIYWVEECHIDG
                     FRFDLASILGRTPSFSEKADFFTALKSHSKLTHIKLIAEPWDIGVEGYQLGQFPEPFY
                     QWNDCYRDDIRRFWLQNQGDWGLLARRLAGSDDVFTKYSVSKSINFITAHDGFTLQDL
                     TSYQQKHNWANGEQNRDGHNSNYSNNHGVEGQTADELVKKQREITACSLLATLFLSAG
                     VPMLLAGDEIGHSQQGNNNSYCQDNEITWLDWENMNHTRLDYVRSLIRLRKKIALLSK
                     QNNWWQEDSVQWLNPSAQLMNIDDWHNQHNGLVVLLQEEWLLLFNANRQPQPFLLPQG
                     NWKVVLEQAQGILMTDTQAVTLNNMGVCVLQKSDVMFANKLETQYA"
     gene            4291..5604
                     /gene="glgC"
                     /locus_tag="PXB27_001161"
     CDS             4291..5604
                     /gene="glgC"
                     /locus_tag="PXB27_001161"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HDL5345960.1"
                     /translation="MLNQESTALVARQDLTNDTLVLILAGGRGSRLYELTDKRAKPAV
                     YFGGSRRIIDFALSNCINSNLLKIGVITQYAAHSLLRHLQRGWSFLPYERNQYIDMLP
                     ARQQLDENTWYRGTADAVYQNMAMMKSHYRPKYVVILAGDHIYKMDYTQMLKDHVDQG
                     AKCTVGCIEVPRSQATEFGVMAVNEKLKVKEFVEKPADPPAMPGKPDSSLASMGIYVF
                     NADYLYEMLEKEASTPGTSHDFGKDIIPKAVEQGVLYAHPFERSCKGRNASGAIYWRD
                     VGTIDSYWAAHMDLVSEDPELDLYDESWPIHGRPQQTAPARFFYNRARARTLDNSLIA
                     GGCVITDAEISNSVLFNRVHVEAESVIEHTVILPQVTIGKNCVIKRAVIDRHCVIPDG
                     LEIGVNAEEDAKRFRVSKNGIVLVTQSMLDKLAGKEANSDIKYTI"
     gene            5727..7163
                     /gene="glgA"
                     /locus_tag="PXB27_001162"
     CDS             5727..7163
                     /gene="glgA"
                     /locus_tag="PXB27_001162"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HDL5345961.1"
                     /translation="MKVLHICSEMYPLIKTGGLADVMGALPFAQQQAGNDVRVLIPYY
                     PKVAQTLGETVEVATIGTFAGTVSVRFAYFNGLGVYVIDAPHLFMRDGNPYHDSNYQD
                     YPDNYKRFALLGYIGAQLAQGLDHWWGTAQVLHAHDWQAGLACAYLKSWGSNVKTLFT
                     IHNIAYIGRFNAFHLAELGLPWEFFHAEGLEFYGEISYLKAGLYFADRITTVSPTYAR
                     EITEEIAGAGLHGLLATRQYQGRLRGILNGVDAEVWNPETDTHIVVNYRANYMHGKAK
                     NKAELQRIFGLPEDKEALLMVMVTRLTEQKGADFLLANIDTLMQHKVQIIVLGSGSPD
                     LENALRYFQHKYPNQIGVKIGYDEALSHQIIAGGDLILVPSRFEPCGLTQLYGLKYGT
                     LPLVRRTGGLADTVVDSHKESIENRSATGFVFDYPDAPAFLEAAMRAISLWQKQKLWS
                     SVRQNALAQDFGWAKVAEQYQNLYHEII"
     gene            7258..9758
                     /locus_tag="PXB27_001163"
                     /pseudo
     CDS             7258..9758
                     /locus_tag="PXB27_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
     gene            complement(9845..10561)
                     /gene="trmJ"
                     /locus_tag="PXB27_001164"
     CDS             complement(9845..10561)
                     /gene="trmJ"
                     /locus_tag="PXB27_001164"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HDL5345962.1"
                     /translation="MQILNQIQIILVETSLPANIGSAARAMKTMGLTHLRLVAPKQPL
                     DEQAEALAAGAKDVLDNAQCYATFDEAIADCQLVIGTSARLRHLQNTLIEPRDCGELV
                     VNRTNLGKVAIVFGRERVGLTNEELLKCQYHLNFPTNPDYGSLNLAMAVQLASYEIRM
                     AYLQNSGEKQPLAADKDYPTAQALEHFFAHTERLYTELGFIRNDSVMLKLRRLYQRAE
                     LETNELNLLRGMLTAVEKAN"
     gene            10741..12279
                     /gene="pntA"
                     /locus_tag="PXB27_001165"
     CDS             10741..12279
                     /gene="pntA"
                     /locus_tag="PXB27_001165"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007525314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HDL5345963.1"
                     /translation="MLIGVPRELLDGETRVAATPKTVEQIKKLGFDVLVEQDAGFKAS
                     FEDNTFLNAGANIGTQQEVWNADIIFKVNAPTEAEIELIKEGVTLVSFIWPAQNPQLM
                     EKLQSKKINVLAMDAVPRISRAQALDALSSMANISGYRAVIEAANAFGSFFTGQITAA
                     GKVPPAKVLVIGAGVAGLAAIGAANSLGAIVRAFDSRPEVKEQVKSMGADFLEIDFEE
                     EGGSGDGYAKVMSEEFNRRAMALYAEQAKEVDIIITTAAIPGKPAPRLITKEMVDSMK
                     PGSVIVDLAAATGGNCEYTKAGEIVVTDNQVKVIGYTDFPARLPTQSSQLYGTNLVNL
                     LKLLAPNKDGQIEINFDDVVLRGVTVVRDGELTWPAPPIQVSAQLQKQAAAPQAVKKE
                     EKPADPRVKYGVMAAAGVLFLWLASVAPAAFLSHFTVFVLACVVGYYVVWNVSHALHT
                     PLMAVTNAISGIIIVGAVLQIAQPTGNFFIDILAFIAILVASINIFGGFKVTQRMLAM
                     FRKG"
     gene            12294..13739
                     /gene="pntB"
                     /locus_tag="PXB27_001166"
     CDS             12294..13739
                     /gene="pntB"
                     /locus_tag="PXB27_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HDL5345964.1"
                     /translation="MSFGFVTAAYIIAAILFIMSLAGLSKHETAKAGCWYGIVGMAIA
                     LVATIFGPQSHGTIWILIAMAIGGFIGVKKALKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYGLHTNALMPANLDETAQAAFLAEQATLANIHNVEVFLGIFIGAVTFSGSLVAFG
                     KLSGKLFGRKVSSAALNIPHKHKWNLAAIIVSVFLMIVFLNNPHNIFPVLIMTIIALV
                     FGWHLVSSIGGADMPVVVSMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYI
                     MCKAMNRSFISVIAGGFGNEVQSGKGDEEYGEHRETKAEEVAEMLKNASSVIITPGYG
                     MAVAQAQYPVAEITAKLREKGVNVRFGIHPVAGRLPGHMNVLLAEAKVPYDVVLEMDE
                     INDDFEETDVVLVIGANDTVNPAALDDPSSPIAGMPVLEVWKAQNVIVFKRSMAVGYA
                     GVQNPLFFKENTQMLFGDAKERVDDILRALN"
     gene            complement(13806..14732)
                     /locus_tag="PXB27_001167"
     CDS             complement(13806..14732)
                     /locus_tag="PXB27_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL5345965.1"
                     /translation="MKPIFLEIRHLRTLIALKESGSVSLAAKRVHLTQSALSHQIKLL
                     EEQYDLTLFERKSSPIRFTPAGERLIKLAYDILPKVIEAELDLARVKQGEVGEVRVAV
                     ECHTCFDWLMPAMDTFRQHWPLVELDIVSGFHTDTVGLLLSHRADLAIVAEEEDSAGI
                     IYKPLFSYEMVAICSKDHPLATKDVWEAEDFKDETLITYPVPDDMLDLLRKILGPAGI
                     KVTRRNSELTIAIIQLVASRRGIAALPYWAVKPYLDRGYVVARKITQQGLYSTLYGAI
                     READATLAYLDDFHDTVKSQSFATLPGLMVLE"
     gene            complement(14842..15957)
                     /gene="asd"
                     /locus_tag="PXB27_001168"
     CDS             complement(14842..15957)
                     /gene="asd"
                     /locus_tag="PXB27_001168"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDL5345966.1"
                     /translation="MQNVGFIGWRGMVGSVLMDRMVEENNFANINPVFFTTSQAGQKA
                     PVFGGKDAGELKNAFDIEELKKLDIIVTCQGGDYTNEVYPKLKETGWNGYWIDAASAL
                     RMKDDAIIVLDPVNQHVISEGLKNGIKTFVGGNCTVSLMLMAIGGLFEKDLVEWVSVA
                     TYQAASGAGAKNMRELLSQMGELEDSVKAELANPASSILDIERKVTAKMRAEDFPTEN
                     FGAALGGSLIPWIDKLLPETGQTKEEWKGYAETNKILGLSDNPIPVDGLCVRIGALRC
                     HSQAFTIKLKKDIPLAEIEQIIAAHNEWVKVIPNDKETTLRELTPAKVTGTLSVPVGR
                     LRKLAMGGEYLAAFTVGDQLLWGAAEPVRRILVQLTQ"
BASE COUNT         4608 a         3106 c         3673 g         4677 t
ORIGIN      
        1 tgaaatttaa gtattaaacg ctttaagcaa ggagtgccga tgcaagacta tacttattct
       61 gaaaaagatg ctttttttgt tgagcgactt tcttcgggtt cttgtaatga tcctttctcc
      121 tacctaggta ttcaccctag caataacggc gttattatta gggtatttct tccttctgcc
      181 atagcttgcc aagtgatcga tttaaacgga aagcctgtgt catcaatgct aaaaattgat
      241 gatagaggct tttttattgc agagctgatt ggtaaaaaaa cagatttttc ttaccgcttg
      301 ttggtggaat accctgattc aacggtggat atagaagacc cttaccgttt taaaacccat
      361 attcaagagc ttgataattg gttgtttgcg gaaggaaaac aacttcgccc gtacgaaaaa
      421 ttaggcgcac atttagtcac acaaaatgaa gttgggggtg tgcattttag cgtctgggcg
      481 ccgaacgctc agcgggtttc cgtggttggg gatttcaacc actggaacgg cttggtacat
      541 tcaatgcgat tccaccccga ttccggtatt tgggatattt tcctgccgaa tgtgaaaaaa
      601 ggggagtgct ataaatttga agtgttggat cgaaacggcg tgctacggtt gaaatccgac
      661 ccttacgcct ttgcctccca attccgccca gaaaccgcat ctattgtttc cggcttgcca
      721 agcattgtgc cagccgagaa aacctgccgt aatgcgaatg ctcccgataa accgatttcc
      781 atttatgaag tgcatttagg ctcttggcga cgcaacattg aaaacaatta ttggcttagc
      841 tatgaagagc ttgccgatga actcattcct tatgtgaaag atatgggctt tacccatatt
      901 gaattgctgc ctatttccga atttccgttt gatggctctt ggggttatca aaccaccggt
      961 atttattcgc caaccagccg ttttggtacg cctgagggct tgagttactt tatccaaaaa
     1021 gctcacgata aaggcattgg tgtgattctt gactgggtag tggggcattt tccggtagat
     1081 gaacacggct tggtttattt tgatggaacg catttatacg aacattccga tccgaaagag
     1141 ggctaccaca aagattggaa taccttaatt tttagctacg gcagaaatga ggtgagcaat
     1201 tatttgtctg gtaatgcact ttattggatt gagcgttttg ggattgatgc gttgcgggtg
     1261 gatgcggtgg cgtcaatgat ttatcgtgat tactcacgtt cagacggcga gtggattcca
     1321 aataaatatg gcggacgtga gaacttagag gcaatcgact tcttacgcca caccaataat
     1381 attttgagag acgaaagcca aggcggtgcg agcattgcag aggagtccac ttcttttgcc
     1441 ggagtgtctc actcaactga aagaggcggt ttgggcttta attataaatg gaatatgggc
     1501 tggatgaacg acactttacg ctatatgcaa aaagatccaa tttaccgccg ttatcaccac
     1561 aatttaatga cttttggtat gacctaccaa tacagcgaac agtttgtgtt accgctttca
     1621 cacgatgaag ttgttcacgg caaaggttca ttgctgaata aaatgccggg ggattgctgg
     1681 caaaaatttg cgaacttacg agcctattat ggctatatgt ggggctatcc gggcaaaaaa
     1741 ttgctcttta tgggcaacga gtttgctcaa ggtaaagagt ggaattttgc cgagagctta
     1801 gattggtatt tactcgggga agaagggggc gaatggcata agggcgtatt gggttgggta
     1861 aaagccctta accatttata cacccaaacc cctgcactgt ttgagcaaga ttacaatcct
     1921 gaaggttttg agtggttagt ggtggacgat cacgataatt cggtgtttgc ttttgagcgt
     1981 aaagccaaaa atggcgattc ggtgattgtg gtgagtaatt tcaccccgat tgtgcgtgaa
     2041 aattaccgtt ttggggtgaa tgaattggca gaatatcagg aaattctcaa ttctgattta
     2101 gcttgttatt gcggtagtaa tgtcagcaat gcaaaccgat ttgccagcga gttaattgca
     2161 tcgcacggta aagcctactc cttgagcatt actttgccac cgctatcgac tatcttcctc
     2221 aaaagagccg cagacaaaaa aacaaaacaa aacaaaacat aacttaaaga aacataaaaa
     2281 ataatatgca ctattttaga ggtaagcctt ttcctttagg caataaactt gatagacaag
     2341 cgggtgaatt tggcacaaat tttgccattg tttcgcaaaa tgcaaccgct attgagttat
     2401 gtctttttga tgaaaatcac cacgaaaccc gtattccgat gttttcttcc ggagatactt
     2461 ggcacatttt tgtagcaggt gtacaagcag ggacaaaata tggctacagg gtgaaaggca
     2521 tacaagatga acaagccggt ttgttattta atccgcaaaa attattgact gatccttacg
     2581 ctaaagcgat tgaagggaag ccggatttaa gtagtgcaga ggcagccgct tggtataaat
     2641 ggaacgatga acgggataat gcctctcttg cacctaagtc tatcgtgatt gatgagcatt
     2701 ttgattggga aaatgatagc cagttattca ctccttgggc agagactatt atttatgaaa
     2761 tgcaggtgaa aggttttagt cagcggaact ttcagattcc aagcgacatt cgaggcactt
     2821 ttgccggttt ggctcaccct gtttcgatag actatttaaa aaccttagga atcaccgcag
     2881 tagaacttct acccgttact taccatattg atgaaaccca tttgcagaaa caagggcttg
     2941 tgaattattg gggctataat gtattaggta attttgctat tgatccgaat ttagcagcag
     3001 ataagcaaaa tcctcttaaa gagtttaaac agctcgtaaa aacattacat caagcacata
     3061 tcgaagtgat tatggatatt gtgtttaacc acacagctga gagtgataaa aacgggccga
     3121 tgctaagttt tagaggcatt gacaatacag cttattggct agataaacag ggcgaatacg
     3181 aaaattggac tggctgtggt aataccttaa atttgagcaa tccttatata ctctgctggg
     3241 caatcgattg cttaatctat tgggtggagg agtgccatat tgatggattc cgctttgatc
     3301 ttgcctcaat tctaggtaga acaccgagtt tttctgaaaa agcagatttt ttcacagcac
     3361 ttaaaagcca ttctaaatta acacatatta agttgattgc tgagccttgg gatattggtg
     3421 tagaaggtta tcaattaggg caattccctg agccttttta tcagtggaat gattgttatc
     3481 gtgatgatat acgccgtttt tggttacaaa accaaggtga ttgggggctt ttggcacggc
     3541 gattggcagg aagtgatgat gtgtttacta aatattccgt ttcaaaaagt atcaatttta
     3601 ttacggcaca cgatgggttt acgttacaag atctcaccag ctatcagcaa aaacataatt
     3661 gggcaaatgg tgaacaaaac cgagacgggc ataatagtaa ctacagcaat aaccacggtg
     3721 ttgaagggca gacagcagat gaattggtaa aaaaacagag agaaatcacc gcttgtagtt
     3781 tgcttgctac gctcttttta tcggctggtg ttccaatgct tttagccggt gatgaaatcg
     3841 gacatagtca acaaggtaac aataatagtt attgccaaga taatgagatc acttggctag
     3901 attgggaaaa tatgaatcac acacggctgg attatgttcg tagtttaatc cggttgcgta
     3961 aaaaaatcgc tttgctttcc aagcagaata attggtggca agaggattcg gtacaatggt
     4021 tgaatccttc tgctcagcta atgaatattg acgattggca taatcaacat aatgggcttg
     4081 tcgttttatt acaggaagaa tggctgcttt tattcaatgc taatcgacaa cctcaaccgt
     4141 ttttactgcc acaaggcaac tggaaagttg tgttggagca ggctcaaggt attttgatga
     4201 cagacacgca agcggttacg ctcaataata tgggtgtatg cgtacttcaa aaaagtgatg
     4261 taatgttcgc taataaattg gagacacaat atgcttaacc aagaatcaac ggctcttgtt
     4321 gcaagacaag atttaaccaa cgatacctta gtattaattc ttgcaggagg acgaggttct
     4381 cgcctatacg aattaaccga taaacgtgca aaaccggcag tctatttcgg tggtagccgc
     4441 cgtattattg attttgcgtt atcaaattgt attaactcga atttattaaa aatcggggtg
     4501 attacccaat atgcagcaca ctctttgttg cgccatttac aacgtggttg gtcattttta
     4561 ccttatgaac gtaaccaata tattgatatg ttaccggcac gccaacagct agatgaaaac
     4621 acttggtatc gtggcacagc ggatgcggtg tatcaaaata tggcgatgat gaaatcgcac
     4681 tatagaccaa aatatgtggt gattttagca ggggatcata tctataaaat ggactacact
     4741 caaatgctga aagatcacgt cgatcaaggt gcaaaatgta ctgtaggttg tatcgaagtg
     4801 cctcgctcgc aagcaacaga gtttggggta atggcggtga atgaaaagct gaaagtgaaa
     4861 gagttcgtcg aaaaaccagc tgatccacct gcaatgccgg gaaaaccgga ttcctcgcta
     4921 gcatcaatgg ggatttatgt atttaatgca gattatctat atgaaatgct ggaaaaagaa
     4981 gcctctacac cgggaacctc acatgatttc ggtaaagata ttattccaaa agcagtagag
     5041 caaggtgtgc tatatgccca cccatttgaa cgttcttgta aagggcgtaa tgccagtggg
     5101 gcaatttact ggcgagatgt cggtacgatt gacagctact gggctgccca tatggattta
     5161 gtttctgaag atccggaatt agatttatat gatgaatctt ggccgattca cggtcgtccg
     5221 caacaaactg caccggcacg ttttttctat aatcgtgcgc gtgccagaac cctagataac
     5281 tcactgattg cgggtggttg tgtgattacc gatgcggaaa tcagtaattc ggtcttattt
     5341 aaccgtgttc acgttgaagc ggagtcggtg attgaacaca cagtgatttt accgcaggtg
     5401 actatcggta aaaattgcgt aattaagcgc gctgtgattg acagacattg tgtaattcct
     5461 gatggcttag aaattggtgt aaatgccgaa gaagatgcga aacgttttcg tgtcagcaaa
     5521 aatggcattg tattagtgac acagtcaatg ttagataaat tagcgggaaa agaagctaat
     5581 tcggatatta aatatacaat ttagtaagag atgatataat gcttcgagtt tgtcatttga
     5641 ggtcgcaagc tcgaagtatt gttatgatta aagcgcttca agcggtctta tttttcaact
     5701 attttacaca tagaaaacgg ttaaaaatga aagttttaca tatttgctct gaaatgtacc
     5761 ctttaattaa aacaggcggt ttagctgatg taatgggggc attgccattt gcgcaacagc
     5821 aagctggtaa tgatgtcaga gtgttaattc cttattaccc gaaagtcgcg caaactttag
     5881 gggaaacggt agaagttgct actatcggca cttttgccgg aacagtctct gttcgatttg
     5941 cttattttaa tggcttaggc gtttacgtta tcgatgcgcc tcatttattt atgcgtgatg
     6001 gtaaccctta ccacgacagc aattatcaag actatccgga taactataaa cgctttgctt
     6061 tattaggtta cattggggcg caattagccc aaggtttgga tcattggtgg ggaaccgctc
     6121 aagttttaca cgcacacgat tggcaagcag ggcttgcttg tgcatatctt aaaagttggg
     6181 gaagcaatgt aaaaacgctc ttcactattc ataatattgc ctatatcggg cgctttaatg
     6241 ccttccattt agcagagctt ggcttacctt gggaattttt ccacgccgaa ggcttagaat
     6301 tttacggtga aatttcttac ctcaaagcag gtttgtattt tgctgatcgc attactaccg
     6361 ttagcccgac ctatgctcgt gaaattactg aggaaattgc cggtgcaggc ttgcatggtt
     6421 tactggcaac ccgtcaatat caaggtagat tgcgtggcat tttaaacggg gtcgatgctg
     6481 aggtgtggaa tccggaaacg gatacacata ttgtggtgaa ttatcgtgcg aactatatgc
     6541 acggtaaagc caaaaataaa gcggaactcc aacgaatttt tggtttgcca gaagacaaag
     6601 aagcattatt gatggtaatg gtgactcgtt taactgagca gaagggggca gatttcttac
     6661 tcgccaatat agatacctta atgcaacata aggtacagat tattgtattg ggaagtggat
     6721 cgccagattt agaaaatgcg ctacgttatt tccaacacaa atacccgaac caaattgggg
     6781 tgaaaatcgg ttatgacgag gctttatcgc accaaattat tgcaggtggt gatctgattc
     6841 tagtaccaag ccgttttgag ccgtgcggat taactcaact ttatgggctg aaatatggca
     6901 ctttaccgct ggttcgccgt accggtggct tagctgatac ggtggtagat agccataaag
     6961 agagcattga aaaccgcagt gccaccggat ttgtatttga ttacccagat gcacctgcgt
     7021 ttttagaggc agctatgcgg gcaatcagct tatggcagaa acaaaaattg tggtcgagcg
     7081 ttcgccagaa tgcccttgct caagattttg gttgggcaaa agtggcagaa cagtaccaaa
     7141 atttatacca cgaaattatt taatttgtat tcacaacatc atttcaagcg gtcgaatttg
     7201 cggatttttt tgtaaaaacg accgcttgca atattaaaaa ttaggagaat ttactcaatg
     7261 agcaggcagt cattaagccc agaaatgatc aaaaatgtta aaaatgcgat tttgcacaaa
     7321 atggtgttcg ctttaggggt cgaaccccgt gatgccagta agcgtaactg gctcaatgca
     7381 accttacgtg tagtgcgtga tttatctacc gaagattggt tacaaacccg ccgtagccag
     7441 gttgcaaatg gtagccgccg tgtttattac ctttcaatgg aatttttaat ggggcgtacg
     7501 ttcagtaacg caatgatttc tgaaggtgtg tatgagctta ttggcgctgc gttaaaagag
     7561 ttagggcaag atctggaaga gattatcaat gaagagggcg tccgggctta ggtaacggtg
     7621 gcttagggcg tttagccgcc tgctatatgg acagccttgc cacaatgaaa attccggcaa
     7681 tcggctacgg tattcaatat gaatacggta tgttccgcca agaaatccgc aacggggagc
     7741 aaattgagca acctgatgaa tggttagaga atgaatttgt gtggccatat ctacgttcaa
     7801 gtaagcgttt cccggtacgc tttggcggta aaacttggcg tgagggcaaa aaagtagtat
     7861 ggcagccgga agaagaaatt accgctcaag ctcacgacca actgatcccg ggctttgaga
     7921 ccacctcaac taacagctta cgtttatggt ctgcacacgc atcgggtaga gggtttggct
     7981 tatctgattt taaccgtggc gattattttg cagcaatggt taaacaaaac agctcggaaa
     8041 acgtctctcg tgtgctttat ccggacgatt ctacctataa cggtcgtgaa ttacgcttac
     8101 gccaagagta tttcctttgc tcggcatctg tacaagatat tatccgccgc cacgaagcgg
     8161 agttcggctc ttgtgtgaat ttagccgaca aagtggcaac tcacttaaat gacacacacc
     8221 caacccttgc ggtgcctgag ttaatgcgta ttttaattga cgaaaaaggc tatggctggg
     8281 aacaagcgtg ggcgatgact cgtaaaattt tcttctacac caatcacact ttgatgagtg
     8341 aagcgttaga aacttggccg gtagaaatgt tagcccgtgt gctaccacgc catttagaga
     8401 ttattttcga cattaacgaa tatttcttag aggaagtgcg tagccaattc ccggaagatc
     8461 acgatttgat ttctcgtgtt tccttaattg atgaacaagg cgatcgccgt gtgcgtatgg
     8521 cgtggcttgc ggtagtcgcc tcagcgaaag taaatggtgt ggcgaaaatt cactcggatt
     8581 taatggttga atctattttt gccgattttg cccgtattta cccaaatcgt tttactaatg
     8641 tgactaatgg ggtgacacct cgccgctgga tcaaaattgc caacccaggg cttgctaata
     8701 ttttagataa atgtatcggc aataagtggc tgactgattt aagtgaattg gaaaaattca
     8761 acgtgtttgt tgatgatgct gatgtgcaag cacaggtggc agcggtgaaa gtagaaaaca
     8821 aacgtcgctt agccaaatat gtcgaagaaa ctcagggaat taaacttaat cctgaagcga
     8881 tttttgatgt acaagttaag cgtattcaca agtataaacg ccagcaaatg aacgtgttgc
     8941 atatcatcac gctttataac cgcatcttga aaaacccgac tgctgattgg acacctcgtg
     9001 tgtttatctt cgcagggaaa gcggcaagtg cttattatgc ggcgaaaaaa gtgattcgtt
     9061 tgattaatga tgtggcaaat gtggtgaata atgaccctcg cattaatgat ttaattaagg
     9121 tggtgtttat tccgaactac ggtgtttcat tagcacaaat gattattccg gcagctgatg
     9181 tttcagagca aatttcctta gcgggtacag aggcctccgg aacctctaat atgaaatttg
     9241 ccctcaatgg tgctttaacc attggtacat tagacggtgc gaatgtcgaa attttagatc
     9301 acgttggtaa agagaacatc tttattttcg gtaatgaagt agagcaggtg gaagagcttc
     9361 gccacaacgg ttactcaccg tatcactact acgaaaaaga cagcgaattg aatgaggcaa
     9421 tttcacaaat tctaaacggt aaattctcac cggaagatcc gtatcgctat caagatttga
     9481 tcttaaattc aggtgactac taccaagcct gtgccgatta tcgtagctat gtggaggctc
     9541 aagaaaaagt ggctcaagcc tatcgtaaca agaaagcttg gacacgttca gccatcatca
     9601 atattgccaa tatgggctat ttctcctcag accgctcggt gatggattat gcgaaaaata
     9661 tttggcatat cgaaccgatg agcgaagaac aattaaaagg cgattctaca atgggcagta
     9721 ttattgaaag ttcaaataaa ctgttgaatt taaaataatg atttagagaa aacggtaaga
     9781 gtaatcttac cgtttttgtt ttagtcaggg attttgcaaa tttctactaa aaattaaccg
     9841 cttgtcaatt tgccttctcc accgccgtga gcattcccct cagtaaattc agctcattag
     9901 tttccagttc ggcacgttgg tataaacggc ggagttttag catcactgaa tcattgcgga
     9961 taaagcctaa ttcggtatat aagcgttcgg tgtgagcaaa gaaatgctct aaggcttgag
    10021 cggttgggta atccttatct gctgcaagcg gttgtttttc gccagaattt tgcaaataag
    10081 ccattcggat ttcataactt gcgagctgta ctgccattgc caaattcaga gaaccataat
    10141 caggattggt tgggaaattc aaatggtact ggcatttcaa taattcttcg ttggttagcc
    10201 ccactcgctc acgtccaaat acaatcgcca ctttgccaag attggttcga tttaccacta
    10261 attcaccaca gtctcttggt tcaatcaggg tattttgcaa atggcgaagt ctggcactgg
    10321 tgccgatcac cagttggcaa tcggcaatgg cttcatcgaa agtggcgtag cattgagcgt
    10381 tatccaacac atcttttgcc ccagcagcaa gggcttcggc ttgttcatca agcggttgtt
    10441 ttggggcgac taatcgcaaa tgggttaagc ccatcgtttt cattgctcgg gctgcggaac
    10501 cgatatttgc cgggagtgaa gtttcaacaa gaatgatctg aatttggttg agaatctgca
    10561 ttggttttgt ttcttttttg ttaaatttag cctgttaata gtgtagcaaa aatttagcct
    10621 gcttaaaatt tgaaaaaatg ggaaataatc ggtttaattg ggcataattt tttacctatt
    10681 tgttttattt agtggtacat ttagagcgtt tttaatcaca acaaccatac aggagtcttt
    10741 atgcttattg gtgtaccaag agagctgtta gacggtgaaa ctcgagtggc ggcaacgcca
    10801 aaaaccgttg agcagatcaa aaaacttggc tttgacgtac ttgtggaaca agacgcaggt
    10861 tttaaagcca gttttgaaga taatacattt ttaaatgcag gtgcaaatat cggcacacaa
    10921 caagaagttt ggaatgcaga cattattttt aaagtgaatg cgccgacaga agctgaaatt
    10981 gagttaatca aagaaggggt aaccttagtc agctttattt ggccggcaca aaatccacaa
    11041 ttgatggaaa aattgcagtc taagaaaatt aatgtgttgg caatggacgc tgtgcctcgt
    11101 atttcccgtg cccaagctct cgatgcttta agttcgatgg caaatatttc gggttaccgt
    11161 gcggtgatcg aagcggcaaa cgcttttggt agcttcttta ctgggcaaat taccgcagca
    11221 ggtaaagtac caccggcaaa agtgttggta attggagccg gtgttgctgg cttggctgca
    11281 atcggggcgg caaatagcct gggggcgatt gtgcgtgcct ttgactctcg tcctgaagta
    11341 aaagagcaag ttaaatcaat gggggctgat ttcctcgaaa ttgattttga agaagaaggt
    11401 ggctcaggtg acggctatgc gaaagtgatg tcggaagagt ttaaccgtcg tgcaatggcg
    11461 ttatatgccg agcaagccaa agaggtggat attattatta ccaccgctgc cattccgggc
    11521 aaacctgctc ctcgtttaat cactaaagag atggttgatt caatgaaacc gggttcagtg
    11581 attgttgatt tagccgctgc aaccggtggt aactgtgaat acaccaaagc cggcgagatt
    11641 gtggtgaccg ataatcaagt gaaagtgatt ggctataccg attttcctgc acgtttacca
    11701 acgcaatctt cgcaacttta tggtactaac ttggtgaatt tactcaaact gttagcacca
    11761 aataaagatg gtcaaattga gattaatttt gatgatgtag tgttgcgtgg cgtaacggtg
    11821 gtgcgtgacg gcgaattaac ttggccggct ccgccgattc aagtatctgc ccaactgcaa
    11881 aaacaagcag ccgcaccaca agcggtcaaa aaagaggaaa aacctgcaga ccctcgagtg
    11941 aaatatggcg tgatggcagc agcaggtgtg ctgttcctat ggcttgcatc tgtggctccg
    12001 gcagcgtttt tatcgcattt taccgtattc gttctcgctt gtgtggtagg gtattatgtg
    12061 gtttggaacg tcagccacgc actacatacc ccattaatgg cggtaacaaa tgcgatttca
    12121 gggattatca tcgtaggtgc ggtattacaa attgcccaac cgaccggtaa tttctttatt
    12181 gatattttgg catttattgc gattttggtt gccagcatca atatttttgg tggctttaaa
    12241 gtaacgcaac gtatgcttgc aatgtttaga aaaggttaag gagaaacgaa actatgtctt
    12301 ttggatttgt gactgctgcg tacattattg cagctattct ctttattatg agcttagccg
    12361 gtctttctaa acacgaaacc gccaaagcag gttgttggta tgggattgtg ggtatggcga
    12421 ttgctttagt ggcaaccatt ttcggcccgc aatcacatgg cacgatttgg atcttaatcg
    12481 caatggcaat cggtggattc atcggggtga aaaaagccct aaaagtagaa atgaccgaaa
    12541 tgcctgaatt agtggcaatt ctacacagct ttgtcggttt agcagcggta ttggttggtt
    12601 ttaacagcta cggacttcat accaatgcat taatgcctgc aaatttagat gaaactgcac
    12661 aagcggcatt tttagcggaa caagccactc ttgccaatat ccacaatgtg gaagtgttct
    12721 taggtatttt cattggtgcg gtaaccttct ccggttcatt ggttgccttt ggtaaactaa
    12781 gcggtaaatt atttggtcgt aaagtctctt ctgcagcact gaatattcca cataaacaca
    12841 aatggaattt ggcagccatt atcgtttctg tttttttgat gatcgtgttt ttaaataatc
    12901 cgcacaatat cttccctgtg ctgattatga caattattgc gttagtcttc ggctggcact
    12961 tagtttcttc tattggtggg gcggatatgc cggtggtagt ttcaatgctg aattcttatt
    13021 caggttgggc agccgcagcg gcaggcttta tgctaagcaa cgatttatta atcgtaaccg
    13081 gtgcattagt gggatcatca ggtgctatcc tttcttatat tatgtgtaaa gcaatgaacc
    13141 gctcattcat tagcgtgatt gccggtggtt ttggcaatga agttcagtct ggcaaaggtg
    13201 atgaagaata cggtgaacac cgtgaaacga aagccgaaga agtggcagaa atgctgaaaa
    13261 atgcaagctc tgtgatcatc acgccgggtt atggtatggc agtggctcaa gcacaatatc
    13321 cggtagcgga aatcaccgca aaattgcgtg aaaaaggcgt gaatgtgcgt tttggtattc
    13381 accctgtggc agggcgttta ccggggcata tgaacgtatt attggcggaa gcgaaagtac
    13441 cttatgatgt agtgctggag atggacgaaa tcaatgacga ttttgaagaa accgatgtgg
    13501 tattggtaat cggtgcaaac gataccgtaa accctgctgc attagacgac ccatcaagcc
    13561 ctattgctgg aatgccggtg cttgaagtat ggaaagcaca aaatgttatc gtgttcaaac
    13621 gttcaatggc agtaggctac gccggtgtgc aaaacccact gttctttaaa gaaaataccc
    13681 aaatgctatt tggtgatgca aaagaacgtg ttgatgatat tttacgggcg ttaaattaaa
    13741 tttaaataaa aaagcgaaca taagttcgct tttttgtgct taggctaaca taaattgtta
    13801 attttttact ctaataccat caatccggga agagtcgcaa aactctgcga ttttacggta
    13861 tcatggaaat catctaagta ggctagggta gcatcggctt ctcgaatagc gccataaagg
    13921 gtgctatata gtccttgctg agttattttt cttgccacca cataccccct atctaaatat
    13981 ggtttaaccg cccaataagg tagtgcagca atgcctcgcc gacttgctac tagctgaata
    14041 atagcaatag ttaactcact attacgccga gtaactttga tgccagcagg gcctaatatt
    14101 tttcgcagta aatctagcat atcatcaggt actggatagg taattaatgt ctcatcttta
    14161 aaatcttcag cttcccatac atcttttgtg gcaagagggt gatctttaga gcaaatagct
    14221 accatttcat atgaaaagag aggtttgtat ataataccag cactatcttc ctcttcagcg
    14281 acaattgcca gatctgcacg atggcttaat agcaaaccaa ccgtatcagt atggaaaccc
    14341 gatacgatat ctaattcaac tagaggccaa tgttgacgga aagtatccat tgccggcatt
    14401 agccaatcaa aacaggtatg gcattctact gcaacacgta cctctccgac ttctccttgt
    14461 ttcactcggg ctaaatctaa ttcagcctca atgactttgg gcaaaatatc gtaggccaac
    14521 ttaattaaac gttcgccagc tggtgtaaag cgaatagggg agctttttcg ctcaaatagc
    14581 gtaagatcat actgttcttc cagtaatttt atttggtgtg aaagtgcgga ttgtgttaaa
    14641 tggacacgct ttgctgcaag agatacgcta ccgctctctt ttaatgcgat taatgtccgc
    14701 aaatggcgga tttcaagaaa aatcggtttc ataatttatt catctaaaag tgaaaaaaga
    14761 tgaaaataat tataacaaac aagcggtcaa attctgcaaa aaatttgcaa aatccaaccg
    14821 cttgtataga gcagtggcga attattgggt taattgaact aaaatgcggc gaacaggctc
    14881 tgctgcaccc cataataatt ggtcacctac ggtaaatgca gctaagtatt ctccacccat
    14941 cgctaattta cgcaagcgac caaccggcac gctcaatgta ccggtcactt tcgccggggt
    15001 caattcacgc aatgtggttt ctttgtcgtt tggaattact ttcacccatt cattgtgggc
    15061 agcaataatt tgctcgattt ccgctaacgg aatatctttt ttcagcttaa ttgtgaatgc
    15121 ttggctgtgg cagcgtaatg ctccaatacg cacgcataaa ccatcaactg gaattgggtt
    15181 atcgcttaaa cctaaaattt tattagtttc tgcataacct ttccactctt ctttagtttg
    15241 cccggtttcc ggtaataatt tatcaatcca aggaattaag ctaccgccca atgccgcacc
    15301 gaagttctcg gttgggaagt cttccgcacg cattttagcc gtaactttac gttcaatatc
    15361 taaaatagaa gaggcaggat ttgctaattc agcttttacg ctatcttcta attcacccat
    15421 ttgagaaagc aattcacgca tatttttcgc accggcaccg gaagcggctt ggtaagttgc
    15481 tacagaaacc cactcgacca aatctttctc aaataaacca ccgatagcca ttagcattag
    15541 gcttacggta cagtttccgc ctacaaatgt tttaatgccg ttttttaagc cttctgaaat
    15601 cacatgttgg ttcaccgggt ctaatacgat aatagcatcg tctttcatac gtaatgcaga
    15661 agccgcgtca atccaatagc cgttccaacc ggtttctttt aattttggat agacttcatt
    15721 tgtataatca ccaccttggc aagttacgat aatgtctaat ttttttaatt cttcaatgtc
    15781 aaaggcgttt tttaattcgc cggcatcttt tccgccaaaa acaggggctt tttgacccgc
    15841 ttgtgaagta gtgaaaaaaa cagggttgat attggcgaag ttattttctt caaccatacg
    15901 atccattaag accgaaccga ccataccacg ccaaccgata aaacctacat tttgcattgt
    15961 gtttgctcct aaaaatgatg ttataaagat tgtgaataaa tacaggattt tagtgcaaaa
    16021 atcaatgttt tttattgatt tgcaaagttt tgacaaaatt taac
//
LOCUS       DANLUQ010000048        15528 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.048, whole genome shotgun sequence.
ACCESSION   DANLUQ010000048 DANLUQ010000000
VERSION     DANLUQ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 15528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15528
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.048"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            46..573
                     /locus_tag="PXB27_001169"
     CDS             46..573
                     /locus_tag="PXB27_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345967.1"
                     /translation="MKLVKFVPALAATLVLAACSNANTQSAPQQAQDAVTKVAEQATQ
                     QVNEIAGNAPRASGAVKVEKATDGAKSIVYRCQSGKRVTANYVFQGEEAKAVNLLVQS
                     GGKTQKIPTLTRDASNQDSTSFKTDKYSWDLDTGFTLSSNGQSGMLTKYGAKTDTIVA
                     KLCDVDKALTSKLNK"
     gene            640..1218
                     /gene="ruvC"
                     /locus_tag="PXB27_001170"
     CDS             640..1218
                     /gene="ruvC"
                     /locus_tag="PXB27_001170"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HDL5345968.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQNGRQLEYLGSGAIRTTAEDLPTR
                     LKRIYAGVSEIITQFQPNMFAIEQVFMAKNPDSALKLGQARGTAIVAAVNHDLPVFEY
                     AARLVKQTVTGIGSADKVQVQDMVTRMLKLSAKPQADAADALAIAITHAHSIQHSLIV
                     AKQSQQNVSSEKEQILALMKTRYSRGRFRLKG"
     gene            complement(1284..2564)
                     /gene="murA"
                     /locus_tag="PXB27_001171"
     CDS             complement(1284..2564)
                     /gene="murA"
                     /locus_tag="PXB27_001171"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDL5345969.1"
                     /translation="MEKFRVYGPFSLNGTVDISGAKNAALPILFAAILAEKTVTLKNV
                     PDLKDVDTTFKILRQLGVTVEKGSDKGVVHIDASKINNYVAPYELVKTMRASIWALAP
                     LVARFQLGQVSLPGGCTIGARPVDMHISGLEKMGAKIELDEGYVKATSNGRLNGARIY
                     MDKVSVGATLSVMMAGTLAKGTTVIENAAREPEIVDTADFLNAMGAKISGAGTDMITI
                     EGVEHLGGCEHSVVPDRIETGTFLVAAAISGGRITCRNTKADTLDAVIEKLREAGMQV
                     DVTENTITLDSLGRRPKAVNIRTMPHPGFPTDMQAQFTLLNTVAEGTSRITETIFENR
                     FMHIPELIRMGAKAEIEGNIAICYGVEQLKSAEVMATDLRASISLVLAGCIATGETIV
                     DRIYHIDRGYEHIEEKLRQIGARIERFSAPFEGE"
     gene            complement(2674..2934)
                     /locus_tag="PXB27_001172"
     CDS             complement(2674..2934)
                     /locus_tag="PXB27_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HDL5345970.1"
                     /translation="MNPSQIEEILKQALSDATEVHAQGENAHFGVIVVSDSIASLSKV
                     KQQQAVYAPLAEYFTTNAIHALTIKVFSVEKWKTERLLNMVG"
     gene            complement(2967..3290)
                     /locus_tag="PXB27_001173"
     CDS             complement(2967..3290)
                     /locus_tag="PXB27_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HDL5345971.1"
                     /translation="MPQKTLQWDIQQNNESLFVKLSGELTRNTLLPLWKQRASFLSPT
                     GKQSLYWDLKEITRIDSAGFTLLAELLNHYHKIIPNSLINTPDSVRTLAALYDLDGWF
                     EQFII"
     gene            complement(3302..3937)
                     /gene="mlaC"
                     /locus_tag="PXB27_001174"
     CDS             complement(3302..3937)
                     /gene="mlaC"
                     /locus_tag="PXB27_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HDL5345972.1"
                     /translation="MLKNIKKIIVTGLVAVSALFSTTAFAETSPYVLMQQTADKLFGD
                     IKANQAKIKSNPEYLRTIVRNDLMPHVHVKYAGQLVLGKNLSAATDAQKEAFFNAFGQ
                     FVEQSYAQVLTQYQDQSVQIESPKSVDGKSIVSIRVNVSGKGAAQPIKLDFKWRKNSK
                     TGEWQAYDMAAEGVSMVATKQNEWAGVIRQKGIDALIAQVAQSAKQPITLK"
     gene            complement(3983..4507)
                     /gene="mlaD"
                     /locus_tag="PXB27_001175"
     CDS             complement(3983..4507)
                     /gene="mlaD"
                     /locus_tag="PXB27_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HDL5345973.1"
                     /translation="MRQSIKYEFWVGLFVLLGLAALVFLGLRVANVQGFSNEKTYTLY
                     ATFDNIGGLKVRAPIKVGGVVVGRVADISLDNKTYTPKVALAVNESFNQIPDTSSLSI
                     KTSGLLGEQYVALNVGFVIEGETAMMKEGDTFVDTNSAMVLEDLIGQFLYGDKKSDKT
                     EDEKASDSAEPKAE"
     gene            complement(4528..5304)
                     /gene="mlaE"
                     /locus_tag="PXB27_001176"
     CDS             complement(4528..5304)
                     /gene="mlaE"
                     /locus_tag="PXB27_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HDL5345974.1"
                     /translation="MVNFISSIGAVVINFIRTLGRSAFMLWGALIGKPEFRKHTPLLI
                     KQLYVLGVQSLLIIMLSGLFIGMVLGLQGYVVLVDFAAESSLGTLVSLSLLRELGPVV
                     TALLFAGRAGSALTAEIGLMKATEQLSSLEMMAVDPLRRIIAPRFWAGVISMPVLAAI
                     FTAIGIWGGSLVGVDWKGVDGGSFWSVMQNSVNTSDLINGFIKSVIFAFAVVWIALFN
                     GYDCVPTSEGISQATTRTVVNASLVILGLDFILTAIMFGG"
     gene            complement(5297..6100)
                     /gene="mlaF"
                     /locus_tag="PXB27_001177"
     CDS             complement(5297..6100)
                     /gene="mlaF"
                     /locus_tag="PXB27_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HDL5345975.1"
                     /translation="MTKTENLVEVKNLTFKRGERVIYDNLNLQVQKGKVTAIMGPSGI
                     GKTTLLRLIGGQILPESGEILFDGRDICKANNKELYEIRQRMGMLFQSGALFTDISTF
                     DNVAFPIREHTRLPEELIRKLVLLKLEAVGLRGAANLMPSELSGGMARRAALARAIAL
                     DPDLIMYDEPFTGQDPISMGVIVELIKELNQALNLTSIVVSHDVKEVLSIADYAYIVA
                     DKRVIAEGTPEILQASEDPQVVQFLAGKADGPVRFHYPAKDYTEELFNG"
     gene            6325..7719
                     /locus_tag="PXB27_001178"
     CDS             6325..7719
                     /locus_tag="PXB27_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HDL5345976.1"
                     /translation="MNKTMKKSFLTAFILGSTIAAMPAYAEATLPTAVNGQAVPSLAP
                     MLEKVRPAVVSIAVEGKTKGDSRRSREIPEEFEFFFGPHADLFGNRSSAPRNFRGVGS
                     GAVINAEKGYVITNNHVIDNADKITVKLEDGREFKAKLVGADPLSDVALIQVEDPKNL
                     TEIKFADSDKLQVGDFTVAIGNPFGLGQTVTSGIISALGRSTGQSDEGYENYIQTDAA
                     VNQGNSGGPLINLNGELIGINTAIISPSGGNAGIAFAIPSNMANSLVTQIIEFGEVKR
                     GMLGIKGGELNADLAKEFNISTQQGAFVSEVLPDSAAAKAGFKAGDVITEINGQKIRS
                     FSELRAKVATSGVGKEIELTYLRDGKEAKAKVTLQSDSEAKVTASNLIPALKGADFNN
                     YNAKGIKGVEISNVEKGSIAEMRSLKKGDIIIGVNRHSVENIADLRKILETKPSVVAL
                     NIVRDGANFYVILQ"
     gene            complement(7771..8532)
                     /locus_tag="PXB27_001179"
     CDS             complement(7771..8532)
                     /locus_tag="PXB27_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C biogenesis protein"
                     /protein_id="HDL5345977.1"
                     /translation="MKTREQLSQENYQQAVKFAQHFADDVRQEFEREAEQRYQFERTQ
                     FEQSVLQNNAQNRPLAKIASLSCVAILLLAMTYYGLSGRYQVVQSGLQQHQDFQQQMS
                     DAHGTSKNERYILSLQNQLRENPNNGDLWYELGQAYALSNDFDSALVCYDNAEKLLGK
                     RAAILGAIATARYYDNGQKMTVEIQAIIEQALQLDKNENASLLLLASDSFLNNNFQQA
                     LDNWRKVLDSNNDSINRRAIIQSMEMARQMLNSQQ"
     gene            complement(8519..8971)
                     /gene="nrfF"
                     /locus_tag="PXB27_001180"
     CDS             complement(8519..8971)
                     /gene="nrfF"
                     /locus_tag="PXB27_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HDL5345978.1"
                     /translation="MRNSIISIGFLQKIRKILPLVFGLLLFVGTVQAEMVDTFQFQNE
                     ADRVRAISLARSLRCLQCQNQNLVESNATAAYNMRIEVYEMVNQGKSNEEIIRIMTER
                     FGSFVNYDPPFTPQTWVLWGLPVGLFSLLFLAILWRTRKNKGNKNENA"
     gene            complement(8961..9494)
                     /locus_tag="PXB27_001181"
     CDS             complement(8961..9494)
                     /locus_tag="PXB27_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HDL5345979.1"
                     /translation="MKKILLLIPLLLLVVIVGFLTVPLMNKDALAPTEDWQGKPFPEF
                     VGKNLLDSNAHLNNNSLPKEPYILNVWVSWCTWCIKEFPILLELKQKGVPIVGLTYSD
                     RPNDALNALKNWGNPFSLIIDDYEKGFLIQTLKVSSAPSSYLIDKHGVVRYQQKGYNP
                     DFAQDFLPKLEALRNEK"
     gene            complement(9491..11395)
                     /gene="nrfE"
                     /locus_tag="PXB27_001182"
     CDS             complement(9491..11395)
                     /gene="nrfE"
                     /locus_tag="PXB27_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfE"
                     /protein_id="HDL5345980.1"
                     /translation="MLPELGYFSLLLAAISAIFQVGFALLGEVRKQYHWLALAPLFTY
                     LQAAFTTFSFSTLAYAFLTDDFSVIYVAAHSNSQLPDFFKFAATWGGHEGSMLFWLTA
                     LVIWSAVFCKFSQKIDRLFANRTLAMLGLIALGFLLFILLVSSPFERGFPPPPEGRDL
                     NPMLQDIGLIFHPPLLYLGYVGFAVSFAMIVAALVSGGMDAAIMRWLRPWTMISWGFL
                     TAGIILGAWWAYYELGWGGWWFWDPVENASLMPWLLGTALVHSLIVSQQRGIFYYWTI
                     LLAIFAFALSLLGTFIVRSGVLTSVHAFAVDPDRGMALLILFFGLSFIALALFAFRAN
                     LWQGKVRFNLLSKETAFLLINGLFSVAASVVLLGTFYPMIFTVMNWGSISVGAPYFNS
                     VFTPLALLLMIVMGFAVVLGWKQISNKQILVKLWLLPIAVGLALGLIYASISQREHFA
                     LQPLPTIFISLAIWIILTHLPYSQKMWQIRPLAMRLAHIGFAVCVIGAMMNSYYGDEI
                     GVRLKPNQAAELAGYTFKYEDYHDLIGPNYTAEQAVFSISRDEQLLATVTPERRYYDV
                     RTMTMAEVGLFHHNLDDIYIVMGDKFGNLEYAFRLHYKPYVQALWLGGMMMVMAALLA
                     LFGYRRENKK"
     gene            complement(11665..12612)
                     /gene="nrfD"
                     /locus_tag="PXB27_001183"
     CDS             complement(11665..12612)
                     /gene="nrfD"
                     /locus_tag="PXB27_001183"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HDL5345981.1"
                     /translation="MNEYVPFQTPNLVWDGTIAIYLFLLGISSGAVQLAIAYRNSGVK
                     MEKPSQNWIIRSAAILGTLPTIIGLLLLIFHLTKPWTFWKLMFNYNFTSVMSMGVMLF
                     QLYMAVLVVWIAIMFKDWIVRYLPVVSGLIDFAEAKLLKPIEFILFVLAAVLGAYTGF
                     LLSALVSYPMLNNPVLPALFLASGASSGIAATFLVILLAGKLSGESHEVHFMHKFEVP
                     IMVTELGLIVAFFVGLHFGGADKSLALTHALNGFWGNVFWIGVMLIGIAIPLVANIFG
                     SHRLKHNVKFIILISVFDLIGVLCLRYFILYAGQLTVAS"
     gene            complement(12612..13289)
                     /gene="nrfC"
                     /locus_tag="PXB27_001184"
     CDS             complement(12612..13289)
                     /gene="nrfC"
                     /locus_tag="PXB27_001184"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007524349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HDL5345982.1"
                     /translation="MTCTRRDFVSGAGAIAVVASTGLATSITLATEKEQKKIRYAMVH
                     DETACIGCTACMDACRTTNKVPEGVSRLEIIRSEPFGEFPNVQYEFFRQSCQHCTNAP
                     CVSVCPTGASFIDPETGIIDVHSDLCVGCQYCIAVCPYRVRFIHPEKKSADKCNFCRD
                     TNLAEGKQPACVEACPTKALTFGDMNDPSSDIFHKVKNNPVYRTKTALGTEPNLYHIP
                     FGKGEHR"
     gene            complement(13289..13945)
                     /gene="nrfB"
                     /locus_tag="PXB27_001185"
     CDS             complement(13289..13945)
                     /gene="nrfB"
                     /locus_tag="PXB27_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HDL5345983.1"
                     /translation="MKKLISKAGRAVALIQAVVFAGFFAISSANALMPEQPKPLWQSE
                     TADDAKVAQDARRDPNKYCTQCHESANATGKPFHHAGKHFQKDVKSPNNGEPLTCISC
                     HGNISESHRKGAKDVMRFNPHGKASNPSLERSVNEQNQVCFACHTAEKLREKFWAHDT
                     HATKIACTNCHEIHPEKDPMKDIPEKDRIKLCTDCHTKIHSGEFKNPEMKATEAKGTK
                     "
     gene            complement(13964..15466)
                     /gene="nrfA"
                     /locus_tag="PXB27_001186"
     CDS             complement(13964..15466)
                     /gene="nrfA"
                     /locus_tag="PXB27_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HDL5345984.1"
                     /translation="MKFLIKSLAVATISILGCLQTALAEEAKTESLTDKAVKHEKLGV
                     KIESANHLFAEKYPLQYDSWKSTAKSTDRGSALEADPRYVILWAGYAFAKDYNKPRGH
                     FYAVTDVRDILRTGAPKDENDGPQPMACWTCKGPDVPRLIEEKGERGYFDPKWAKYGA
                     EIVNSIGCADCHDTTSEEFKQGKPALRVARPHVLRALNTVGWKFEDLDKHGKRPAVCA
                     NCHVEYYFKNKTDVTFPWDKGVDVDSIEKYYDEINFTDWTHALSKAPMLKTQHPDFEV
                     WSQGTHGKNGVTCIDCHMPKVKDKDGKVYTDHKIGNPFDQFEATCKTCHEQSKETLEK
                     RVKEYKHEVKEAMIRLEDQLVKAHFEAKAAWEAGATQEEMKEILMAIRHAQWRWDYSA
                     AGHGNHFHAPDVMLRTIATGIDRAADARAKLGVVLAKHGVTTPVAIPDISTKEKAQLA
                     IGLDIPKEQAAKDEFLKTVVPQWEKEARAKGLLPAEEADKPVAAPKAEAK"
BASE COUNT         4617 a         3482 c         3047 g         4382 t
ORIGIN      
        1 tagtttatat gctactactt tactataaca ttttaaggag agtctatgaa attagtgaaa
       61 tttgtccctg ccctagcagc aactttagta ttagccgctt gctctaatgc aaacacacaa
      121 tctgctcctc agcaagctca ggacgctgtg actaaagtgg ctgaacaagc aacacagcaa
      181 gtgaatgaaa ttgcaggtaa tgcacctcgt gcaagtggtg cagtaaaagt agaaaaagca
      241 actgatggtg caaaatcaat tgtatatcgt tgccaaagtg gtaaacgtgt aactgcgaat
      301 tatgtcttcc aaggagaaga ggcaaaagca gtaaatctat tagtccaaag cggcggtaaa
      361 acacaaaaga tcccaacatt aactcgtgat gcctcaaacc aagattctac ttcattcaaa
      421 acggataaat attcttggga tttagacaca ggctttacct tatcatctaa tggccagtca
      481 ggtatgctaa ccaagtatgg tgctaaaaca gataccatcg ttgctaaatt atgtgatgta
      541 gataaagcat taacctcaaa attaaataaa taatctttct tgattttact actcataagc
      601 gagaagataa tatcttctcg cttattttat tgcacaaaga tggctattat tttaggaatt
      661 gaccccggct caagggtaac aggttacggt gtgattcgcc aaaacgggcg acagctggaa
      721 tatttaggaa gtggggctat tcgaaccacg gctgaagatc tgcctacccg cttaaaacgg
      781 atttatgcag gcgtgagtga aatcatcact caatttcaac caaatatgtt cgccatagag
      841 caggtattta tggcaaaaaa ccctgattct gccttaaagc tcgggcaggc tcgaggaaca
      901 gcgattgtag cagcggttaa tcacgattta cctgtgtttg aatatgccgc ccgattagtc
      961 aaacaaaccg ttacgggaat tggttctgct gataaagtac aagtgcaaga tatggtaacc
     1021 cgtatgctta aactctccgc caaaccacaa gccgatgcgg ctgatgcatt agccattgcg
     1081 attacccacg ctcattcaat ccaacattca ctgattgttg ctaagcaaag ccagcagaat
     1141 gtgagtagtg agaaagagca aattttagca ctaatgaaaa cccgttatag ccggggaagg
     1201 tttaggttaa aaggctaact caatataaaa tgaccgcttg gaactaatgt ttcaagcggt
     1261 cattttttga gaaaatttta caattactcg ccttcaaaag gagctgagaa acgttcaatt
     1321 ctcgccccaa tctgacgtaa tttttcttcg atgtgttcat agccacggtc tatgtggtaa
     1381 attctatcca caatcgtttc acccgttgca atacagcctg ccaacactaa gctgatggaa
     1441 gcacgcaagt cagttgccat tacttctgct gattttaact gctctacacc atagcaaatt
     1501 gcaatattac cttcaatttc cgctttggca cccatacgaa tcagctcagg aatatgcata
     1561 aaacggtttt caaaaatcgt ttctgtaata cggcttgtgc cttctgcaac tgtatttaat
     1621 aaggtaaatt gagcttgcat atcagttggg aaacctgggt gtggcatagt gcgaatgttc
     1681 actgctttcg ggcgacgacc tagagaatca agcgtgatag tattttccgt cacatcaacc
     1741 tgcattccag cttcacgtaa tttttcgatc actgcatcaa gagtgtctgc tttggtgtta
     1801 cggcaagtaa ttcgaccacc ggaaatagcc gctgccacta aaaatgtacc ggtttcaata
     1861 cgatccggca ctacgctgtg ttcgcaaccg cctaaatgct caaccccttc aatggtaatc
     1921 atatcggtac ctgcaccaga aattttagct cccatcgcat ttaagaaatc ggcagtatct
     1981 acaatttcag gctcacgagc agcattttca ataacagtag taccttttgc tagagtacct
     2041 gccatcatca ccgataaagt tgctcctacg ctcactttat ccatatagat tcttgcaccg
     2101 tttaaacgac cgtttgaagt tgctttgaca tagccttcat caagctcgat tttcgccccc
     2161 attttttcaa gaccggaaat gtgcatatct accggtcttg ccccgatagt acaaccgccc
     2221 ggtaaagaaa cttgcccaag ttggaaccgg gcaactaatg gagctaacgc ccaaattgaa
     2281 gcacgcattg ttttcactaa ttcgtaaggt gcaacgtaat tattgatctt actcgcatca
     2341 atatgtacca cgcctttatc gctacctttt tcaaccgtta cccctaattg gcgtaagatt
     2401 ttaaaagtgg tgtccacatc ttttaaatca ggcacatttt ttaacgtaac tgttttttca
     2461 gccaaaatag cggcaaataa aatagggagt gcggcatttt tcgcacctga aatatcgaca
     2521 gtaccattta aagaaaatgg accatacaca cgaaattttt ccatttggga gatcctaaaa
     2581 aataataatt tgtaggggcg aaaaattttt cgcccaattt tccaacgttt acaaaagagt
     2641 ttgggcgaat agtgattcgc ccctacattt catctatcca accatattta gcaaacgctc
     2701 agttttccat ttttccacgc tgaatacttt aatggttaaa gcgtgaatcg cattagtagt
     2761 gaaatactca gctaaagggg cataaacagc ttgctgttgt tttacttttg aaagagaagc
     2821 aatgctgtca cttactacaa taacgccaaa gtgggcattc tcgccttgag cgtggacttc
     2881 tgttgcatca gataatgctt gttttaaaat ttcttcaatt tgtgatggat tcattctgac
     2941 ttcctaaaat atgattggta actcactcaa ataataaatt gctcaaacca accgtctaaa
     3001 tcgtataatg cagccaaagt tctgacagaa tctggcgtat taatcaaact gtttggtatg
     3061 attttatggt aatgattcag taattcagca agtaaggtaa aaccggcaga atcaatccga
     3121 gtgatttctt ttaaatccca atataggctt tgtttccctg ttggagataa aaaagaagca
     3181 cgttgcttcc ataaagggag caacgtgtta cgagttaatt cgcctgatag tttcacaaaa
     3241 agactttcgt tattttgctg aatgtcccat tgtaatgttt tttgaggcat aactaaacta
     3301 cttattttaa agtgataggc tgttttgcag attgtgccac ttgtgcaatt aaggcatcaa
     3361 tccctttttg acgaatcaca cccgcccatt cgttttgctt ggttgcaacc atacttactc
     3421 cttcagcggc catatcgtat gcttgccatt cacctgtttt gctgttttta cgccatttaa
     3481 aatctaattt gatcggctgt gcagcacctt tacctgacac gtttacacga atgcttacga
     3541 tagatttacc atccaccgat ttcgggcttt caatttgcac actttgatct tgatactggg
     3601 ttaacacctg agcataagac tgctcaacaa attgaccaaa agcattaaag aatgcctctt
     3661 tttgtgcatc agtcgcagcc gataagtttt tccctaacac taattggcct gcatatttca
     3721 cgtgaacgtg tggcattaaa tcgttacgca caatggtacg caaatattca gggtttgatt
     3781 taattttcgc ttgatttgct ttaatatcgc caaataattt atctgcggtt tgttgcatta
     3841 atacataagg gctagtttca gcaaatgcgg tggttgaaaa taatgcagaa actgccacta
     3901 agcctgttac gataattttt ttaatgtttt ttaacattat tgatgctctc caaaactcta
     3961 aattagaggt tgttgtactt aattactctg cttttggttc agcagaatca cttgcttttt
     4021 catcttcagt tttatctgac tttttatcac catataagaa ctgaccgatt aaatcttcta
     4081 acaccattgc agaattggta tctacaaaag tatcaccttc tttcatcatt gcagtttcac
     4141 cttcgattac gaagccaaca tttaacgcaa cgtattgctc gcctaataag cctgaggttt
     4201 taattgagag cgagctggta tcgggaattt gattaaaact ttcatttacc gctaatgcca
     4261 ctttcggggt gtaggttttg ttatcaagtg aaatatcagc cactcggcca actaccacac
     4321 cgccaacttt aataggcgca cgaaccttta gtccaccaat attatcaaaa gttgcataca
     4381 gggtgtaagt tttctcattt gagaagccct gcacatttgc tactcttaag cctaaaaaga
     4441 caagagctgc aagcccaagt agtacaaata agcctaccca aaattcatat ttaattgatt
     4501 gacgcattgt ctgttccttt caaaaaatca accgccgaac attatcgcag ttaaaataaa
     4561 atccaatcct aaaatcacca gtgaagcatt taccaccgtt cttgtggtcg cttggctgat
     4621 tccttctgaa gtcggtacgc aatcgtagcc gttaaataag gcaatccaga ccaccgcaaa
     4681 ggcaaaaatc acacttttaa taaagccgtt aattaaatcg ctggtattta ccgagttttg
     4741 cattaccgac caaaaactgc caccatcaac gcctttccaa tctaccccga ctaatgagcc
     4801 gccccaaatt ccgatagcgg taaaaatagc agctaacacc ggcattgaaa tcacgcctgc
     4861 ccaaaagcga ggagcgatga tacgtctaag cggatcaacc gccatcattt caagactgga
     4921 aagttgctct gtggctttca tcagcccgat ttctgcggtc aatgccgaac cggctcgccc
     4981 tgcaaataat aatgcagtaa ctacaggacc aagttctcgt aaaagcgaga gtgaaaccaa
     5041 agttcccaag ctactttcgg cggcaaaatc gaccaatacc acatagcctt gtaagcctaa
     5101 caccattccg ataaataagc ccgacagcat aataattaag agcgattgca ctcctaacac
     5161 ataaagctgt ttaatcagta acggggtatg ttttctaaat tcaggtttac cgattaatgc
     5221 tccccataac ataaaagcgg aacgccctaa tgtacggata aaattaatca ccaccgcccc
     5281 gatagagcta ataaaattaa ccattaaata gctcctccgt ataatctttt gctggataat
     5341 ggaaccgcac cggaccatca gctttaccgg ctaagaattg taccacttgc ggatcttcgc
     5401 ttgcctgtaa aatttccggt gtaccttcag caatcactcg tttatctgcc acaatataag
     5461 cataatcggc aatacttaat acttctttca catcgtgaga caccacaatg gaggtcaaat
     5521 tcaatgcctg atttaactct ttaatcagct cgacaatcac acccatacta atcggatctt
     5581 gcccggtaaa cggctcatca tacataatca gatccggatc taacgcaatc gctcgagcaa
     5641 gggcggcacg tcttgccata ccgccggaaa gttcggacgg cattaaattt gccgcacctc
     5701 gcaacccaac cgcctcgagc ttcaataaca ccaatttgcg aattagctct tcgggtaaac
     5761 gggtatgctc ccgaatcggg aaagccacat tatcgaaggt agaaatatcg gtaaataacg
     5821 cccccgattg aaacaacatc cccattcgtt ggcgaatttc atacaactct ttgttattgg
     5881 ctttgcaaat atctctgcca tcaaataaaa tttcgcccga ttcaggtaaa atctgcccac
     5941 cgattagcct caacagggtg gttttaccaa tcccagaagg ccccataatg gcagtcactt
     6001 tacctttctg cacttgcaga ttcaggttat cgtaaatcac tcgctcccct cgcttgaagg
     6061 tcaagttttt tacttcaacc aaattttccg tttttgtcat caaataggac tcaaattaat
     6121 aaaagtgata tttatggtct atcaaatttg ccagaatgtc aaaaaacttt taataatctg
     6181 acaaaaatct gcctttacaa gcggtcagat ttgctaaatt ttttgcaaac cggctgaact
     6241 gaaaaagaaa ggtcttgtct tagccaatac gagcgttttc actcgtagat tataaaaaca
     6301 taggatatga ggaacattca caatatgaac aaaacaatga aaaaatcatt tttaactgca
     6361 ttcattttag gctcaaccat tgccgcaatg ccggcgtatg cagaagcaac attacccaca
     6421 gccgtaaatg ggcaagctgt gccaagcctt gcaccaatgc tggaaaaagt tcgcccggcg
     6481 gtggtgagta ttgcagtaga aggtaaaacc aaaggcgata gccgccgcag tcgtgaaatt
     6541 ccggaagaat ttgaattttt cttcggtcct cacgccgatt tattcggcaa ccgctcatca
     6601 gctccacgca atttccgtgg cgttggatca ggtgcagtca tcaatgccga aaaaggttat
     6661 gtgattacca ataaccacgt gatcgacaat gccgataaaa ttaccgtaaa acttgaagac
     6721 ggacgtgaat ttaaagctaa attagttggt gctgatcccc tttcagatgt ggcgttaatt
     6781 caagttgaag atccaaaaaa cttaactgaa attaaattcg ccgattccga taaattacaa
     6841 gtgggcgatt ttacagttgc aatcggtaac ccgtttggct taggacaaac tgttacttca
     6901 ggcatcatct cggcacttgg tcgttcaacc ggtcaatccg atgaaggcta tgaaaactat
     6961 attcaaaccg atgcggcagt taaccaaggt aactctggcg gcccgttaat taacttaaac
     7021 ggcgaattaa tcggaattaa caccgctatt atttctccaa gtggtggcaa tgccggtatc
     7081 gcctttgcga ttccaagtaa tatggcaaac agcttggtga ctcaaattat tgagtttggt
     7141 gaagtaaaac gtggaatgtt aggcattaaa ggcggtgaat tgaatgctga ccttgctaaa
     7201 gagttcaata tttccaccca acaaggggca tttgtaagcg aagtcttacc ggattctgct
     7261 gctgctaaag ccggctttaa agcaggtgat gtgattactg aaatcaacgg tcaaaaaatc
     7321 cgtagtttca gcgaattacg agcaaaagta gctacttcag gtgtaggtaa agagattgaa
     7381 cttacttacc tgcgtgacgg caaagaagcc aaagcaaaag tgaccttaca atcggattct
     7441 gaagccaaag ttactgcttc aaacctcatt ccggcattaa aaggggcaga ttttaacaac
     7501 tacaatgcca aaggcattaa aggggttgaa atttctaacg tagaaaaagg ctcgattgct
     7561 gaaatgcgaa gcctcaaaaa aggcgatatt atcatcggcg tgaaccgcca ctcggtagaa
     7621 aacattgccg atttacgcaa aatattagaa accaagccat ctgtggttgc actcaatatt
     7681 gtgcgtgacg gtgcaaattt ctatgtgatt ttgcaataaa ccttttgatc cactcaaaca
     7741 aaagccgatg acaaaaatca tcggcttttg ttattgctgg gaatttaaca tctgtcgtgc
     7801 catttccata ctttggataa tcgctctgcg gttgatagaa tcattattgc tatctaacac
     7861 tttacgccaa ttatcgagag cttgttgaaa attgttattt aaaaagctgt cggaggctag
     7921 caggagtaaa ctggcatttt catttttatc caactgtagg gcttgctcga taatggcttg
     7981 aatttcaacg gtcattttct gcccgttatc gtaataacga gcggtggcaa tcgcccccaa
     8041 aatagccgct cgttttccga gtagcttctc ggcattatcg tagcaaacca gtgccgaatc
     8101 aaaatcattg cttaacgcat aagcctgccc tagttcgtac cataaatcac cgttattcgg
     8161 gttttctcga agctggtttt gcagactcaa aatatagcgt tcatttttcg atgtgccgtg
     8221 tgcatcgctc atctgttgtt gaaaatcttg atgttgttgc aaacctgatt gcaccacttg
     8281 ataacgtccg gaaagcccat agtaagtcat tgctaacaac aaaatcgcca cgcaactcag
     8341 gcttgcgatt ttagcaagcg gtcgattttg agcattattt tgcaaaacgc tctgttcaaa
     8401 ttgcgttcgt tcaaattggt aacgttgctc tgcctcacgc tcaaactctt gtctgacgtc
     8461 atctgcaaaa tgttgggcaa atttaaccgc ttgttggtag ttttcttggc ttaattgctc
     8521 acgcgttttc atttttgttg cctttatttt tgcgagttcg ccacaaaata gctagaaata
     8581 acaggctaaa caagccaacc ggcaagcccc ataataccca tgtctgtggc gtgaatggtg
     8641 gatcgtaatt cacaaaactg ccgaaacgct cggtcataat gcggataatt tcctcattac
     8701 ttttcccttg attcaccatt tcatacactt caattcgcat attgtaagcc gccgtcgcat
     8761 tggattcgac caaattttgg ttctgacatt gcaaacatcg caacgaacga gccagtgaaa
     8821 tcgcccgtac ccgatccgcc tcattttgaa attgaaaggt gtccaccatt tcagcttgaa
     8881 ccgtgccgac aaatagcaac aagccaaata caagcggtaa tattttgcgg attttttgca
     8941 aaaagccgat ggaaattatg ctatttctca tttctgagag cctctaattt gggtaggaaa
     9001 tcctgtgcaa aatcagggtt gtaacccttt tgttggtaac gcactacacc gtgtttatca
     9061 atcaaataac tggaaggtgc agaagagact ttcagggttt ggatcaaaaa gcctttttcg
     9121 taatcatcaa taattaaact aaacggattt ccccagtttt tcaatgcgtt gagggcatca
     9181 tttgggcggt cgctataagt taagccgaca atcggcacgc ctttttgttt tagttccaac
     9241 aaaatcggga actctttaat gcaccaagta caccagctca cccacacatt gagaatataa
     9301 ggctctttcg gcaagctgtt gttatttaaa tgcgcgttac tatcaagtaa gttcttgcct
     9361 acaaactctg gaaacggctt gccctgccaa tcttcagtgg gagcaagggc atctttattc
     9421 attaacggca cagtcaaaaa gccaacaata actacaagta acaggagcgg aataagtaac
     9481 aggatttttt tcattttttg ttttctctac gataaccgaa taatgccaac agggcggcca
     9541 ttaccatcat cataccacct aaccataagg cttgcacata aggcttatag tgcaaacgga
     9601 aggcatattc taaattgccg aacttatccc ccatcacaat ataaatatcg tctaaattgt
     9661 ggtggaaaag ccctacttct gccattgtca tcgttcttac atcataatag cgtctttccg
     9721 gtgtgacggt tgccagcaac tgttcatctc ggctgataga aaaaacggct tgctcggcgg
     9781 tgtagttcgg accgatgaga tcgtgataat cttcatattt aaaggtataa ccggcaagtt
     9841 ccgctgcttg attcggcttt aaccgcacgc cgatttcatc gccgtaataa ctgttcatca
     9901 ttgccccaat cacgcaaacc gcaaagccga tatgggctaa ccgcatcgca agcggtcgaa
     9961 tttgccacat tttttgcgaa tagggcaaat gagtgagaat aatccagatc gcaaggctga
    10021 taaatatggt gggtaacggc tgcaaagcaa aatgctctct ttgcgagatg gaggcataaa
    10081 tcaaccctaa cgctaaaccg actgcaatcg gcagcaacca aagttttacc aatatctgct
    10141 tatttgaaat ctgtttccag cccagcacca ccgcaaagcc cattactatc attaacagta
    10201 acgctagtgg cgtgaatacg ctattgaaat agggggcccc cactgaaatc gatccccaat
    10261 tcatcactgt gaagatcatc gggtagaaag tccccagcaa taccacagaa gcagccacac
    10321 taaataagcc gttaatcaat aaaaatgccg tttctttaga aagtaagttg aaacgcactt
    10381 tgccttgcca gagattcgct cggaatgcaa acagagcaag ggcaatgaaa cttaagccga
    10441 agaataagat cagcagtgcc attcctctgt cgggatcgac cgcaaaggca tgaaccgagg
    10501 tcagtacacc cgaacgaaca ataaacgtgc ctaataggct taaagcaaag gcgaaaatgg
    10561 caagtagaat cgtccaataa tagaaaatgc cacgctgttg gctcacaatt aaactatgca
    10621 ccaatgcggt gccaagtagc caaggcatta gggaggcatt ttccaccgga tcccaaaacc
    10681 accagccacc ccagccgagt tcgtaatatg cccaccacgc ccctaaaata atgccggcgg
    10741 tgagaaatcc ccacgaaatc atcgtccacg gacgaagcca acgcataatc gcagcgtcca
    10801 ttccgcctga aacgagggcg gcgacaatca ttgcaaagct caccgcaaag ccgacatagc
    10861 ccaagtagag caaaggcggg tgaaagatga gcccaatatc ttgcaacatt gggttgagat
    10921 ctcgcccttc aggtggtggt ggaaagccac gttcaaatgg gctggaaacc aatagaatga
    10981 aaagtagaaa acctagtgca attagcccta acatcgctaa ggttcggttg gcaaacaagc
    11041 ggtcaatttt ttgcgaaaat ttgcaaaata ccgcagacca aatcacaaga gcggtgagcc
    11101 aaaacagcat tgagccttcg tgtccgcccc aagtggcggc aaatttaaaa aaatcgggga
    11161 gctggctgtt ggaatgagcg gcaacgtaga taaccgaaaa atcatcggtt agaaaagcat
    11221 aagctaaggt agaaaaggaa aatgtggtaa aagcagcttg caaataagtg aaaagcggag
    11281 ccaatgccag ccaatgatat tgcttgcgaa cttcgcctag aagtgcaaaa ccaacttgaa
    11341 aaatggcact aattgcagcc aataaaagtg aaaaatagcc gagttccggt agcataaaaa
    11401 atccaaaaaa tgaccgcttg tgaatgtaaa aaaagccctc tccacttgtg ggagagggac
    11461 agatttttct tcgttcagaa gaaaaatcag ggagagggga attaaataat tttgctaaaa
    11521 ataacaatac tccctctctc tgaaaaatat ttctgaacga aatatttttc ggtctcaata
    11581 ctcgctacgc tcgctccttc ggagccgttg gcaaagccaa cgttcaaatg taaacatttg
    11641 tcccacaagg ggagagagta tatcctaact tgcaaccgtt agttgccctg cataaaggat
    11701 aaagtaacgc aagcagagta caccgattaa gtcgaaaacg gaaattaaga taataaattt
    11761 cacgttatgt ttcaagcggt ggctaccaaa aatattggca acaagcggaa tcgcaatacc
    11821 gattaacatt acgcctatcc agaacacatt cccccagaag ccgttaagtg catgggttaa
    11881 agcgagcgat ttatctgctc caccgaagtg taagcccaca aagaacgcta caattaagcc
    11941 taattcggtt accataatcg gcacttcaaa tttgtgcata aagtggactt cgtggctttc
    12001 accggataac ttgcctgcga gtaaaatcac taggaaagtt gctgcaatac ctgacgatgc
    12061 ccctgatgcc aagaacaatg ccggtaacac agggttattc agcattggat aactcactaa
    12121 cgccgagagt aagaaaccgg tgtaagcacc aagtacagca gccaatacga ataagataaa
    12181 ttcaattggt tttaacagct tcgcttcggc aaaatcgatc aaaccagaga ccacaggtag
    12241 ataccgaaca atccaatctt taaacattat ggcgatccaa accaccaata ctgccatata
    12301 aagttggaat aacattaccc ccatcgacat tactgaggta aagttatagt tgaacattaa
    12361 tttccagaat gtccaaggtt tggttaagtg gaaaatcagt aacagtaagc cgataatggt
    12421 tggtaatgtg ccaagaatag ccgcactacg aataatccag ttttggcttg gtttttccat
    12481 tttgacaccg ctattacggt aagcaatcgc taactgaacc gcacctgatg aaataccaag
    12541 taggaataag taaatagcga tagtgccatc ccaaacaagg tttggggttt ggaaaggtac
    12601 atattcattc attatctatg ttctccttta ccaaatggaa tgtgatataa attcggctcc
    12661 gtgcctaatg ccgttttggt gcgataaacc ggattatttt tcactttatg gaaaatatcg
    12721 ctacttggat cattcatatc accaaaggtt aaagctttag tcggacaagc ctctacacaa
    12781 gcaggctgtt tgccttctgc aagattggta tctcggcaaa agttacattt atccgccgat
    12841 tttttctcag ggtgaataaa acgtacacga tacgggcaaa ccgcaataca gtattggcaa
    12901 ccgacacaca aatctgagtg tacatcaata atcccggttt caggatcgat aaatgaagca
    12961 ccggtcgggc aaacactcac acaaggtgca ttggtacagt gttggcaaga ttggcggaaa
    13021 aactcatatt gtacgttcgg aaactcaccg aaaggctcac tacggataat ttctaaacga
    13081 gaaacccctt ccggcacttt gttggtggtg cgacaagcgt ccatacaagc cgtacagccg
    13141 atacaggcag tttcatcatg aaccatcgca tagcggattt tcttttgttc tttctcagta
    13201 gcaagagtta tggaggtggc aagccctgta cttgccacaa ctgcgatagc acctgcccct
    13261 gagacgaaat ctctacgggt gcaagtcatt attttgttcc ttttgcttct gtggctttca
    13321 tttcaggatt tttaaattcg cctgaatgaa ttttggtatg acaatccgta caaagtttaa
    13381 tgcgatcttt ttcaggaatg tctttcatcg gatctttttc cgggtgaatt tcatgacagt
    13441 tggtacaagc aattttagtt gcgtgcgtgt cgtgagccca gaatttttca cgcaatttct
    13501 ctgccgtatg gcaagcaaaa cagacttgat tttgctcatt taccgaacgt tcaagtgatg
    13561 ggttacttgc tttaccgtgt gggttaaaac gcattacgtc ttttgcccct ttgcggtggc
    13621 tttcagaaat attaccgtgg caacttatac aagtgagtgg ctctccatta ttcgggcttt
    13681 tcacatcttt ttggaaatgt ttgcctgcgt gatggaaagg cttacctgtt gcgtttgccg
    13741 attcgtgaca ttgagtacaa tatttatttg gatcacggcg tgcatcttga gctacttttg
    13801 catcatcagc cgtttctgat tgccaaagag gttttggctg ctcaggcatt aacgcattcg
    13861 cactggaaat tgcaaaaaaa cctgcaaata cgaccgcttg tatgagggcg actgctcgtc
    13921 ctgcttttga gattaatttt ttcatagttc acctcaagct acattatttt gcttctgctt
    13981 ttggtgctgc taccggtttg tctgcttctt ctgcgggtaa taaaccttta gcacgagctt
    14041 ctttttccca ttgaggaacc acagttttta agaactcatc ttttgctgct tgctctttag
    14101 gaatatctaa accaatagca agttgtgctt tctctttagt tgagatgtcc ggaatcgcca
    14161 ctggcgttgt tacaccgtgt ttcgctaaca ctacacctaa tttagcacgg gcatctgctg
    14221 cacggtcgat acctgttgcg atggtacgta acattacatc aggtgcgtgg aagtggttac
    14281 cgtgacctgc cgcagagtaa tcccaacgcc attgagcgtg acgaattgcc attaagatct
    14341 ctttcatctc ttcttgagtt gctccagctt cccaagcagc tttcgcttca aagtgagctt
    14401 tcactaattg atcttctaag cgaatcattg cctcttttac ttcgtgttta tactctttta
    14461 cacgtttttc caatgtttct ttgctttgtt catgacaagt tttacaagtc gcttcaaatt
    14521 ggtcaaacgg attaccgatt ttgtggtcag tataaacttt gccgtctttg tctttcactt
    14581 ttggcatatg gcaatcaata caggttacac catttttccc atgtgtacct tgtgaccaaa
    14641 cttcaaaatc tgggtgctgc gtttttagca tcggagcttt cgataaagca tgagtccagt
    14701 ctgtaaaatt gatttcatcg tagtattttt caatgctatc tacatctaca cctttatccc
    14761 aagggaaagt tacatctgtt ttatttttga aatagtactc cacatggcag ttagcgcaca
    14821 cagcagggcg tttaccatgt ttatctaaat cctcaaactt ccaaccaacc gtattcaatg
    14881 cacgtaatac gtgaggacgt gcaacacgta gtgcaggttt accttgttta aactcttctg
    14941 atgtcgtgtc atggcagtct gcacaaccta ttgagtttac aatttcagca ccatatttcg
    15001 cccatttagg atcaaaatat cctcgctcac ctttctcctc aattaagcgt ggaacatcag
    15061 ggcctttaca tgtccaacaa gccataggtt gtggaccatc attttcatct ttaggcgcgc
    15121 cggtgcgcaa aatatcacgc acatcggtaa ctgcataaaa gtgacctcgt ggtttattat
    15181 aatctttcgc aaaagcatag cctgcccaca aaataacata acgagggtca gcctctaacg
    15241 cacttcctcg atctgtggat tttgcagttg acttccaaga atcgtattgt agcggatatt
    15301 tttctgcaaa taggtggttt gcagactcaa ttttcacgcc taatttttca tgcttaactg
    15361 ctttatctgt taatgattca gttttcgcct cttcagctaa agcagtttgt aaacacccca
    15421 atattgaaat tgttgccaca gctaggcttt ttattaaaaa tttcattttg gctctcccaa
    15481 tcaggtaaaa ataattagag aaaaataaga atttttccta ttaaaatt
//
LOCUS       DANLUQ010000049        14934 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.049, whole genome shotgun sequence.
ACCESSION   DANLUQ010000049 DANLUQ010000000
VERSION     DANLUQ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14934
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.049"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            270..701
                     /locus_tag="PXB27_001187"
     CDS             270..701
                     /locus_tag="PXB27_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3577 domain-containing protein"
                     /protein_id="HDL5345985.1"
                     /translation="MTTQTSTQKNYFNLHTTGIGYLNDIRLVEPKKGNPFYACRIAAL
                     VGESDSPEYRYFDMNVVGKETETLIKRCQKAVSEEKKVLVSFVMADLWFDTFTYSTDS
                     KFHKKGDTGVSVKGRLIRIKMIKIDGEIAYQEKPKSDEQSE"
     gene            719..1711
                     /locus_tag="PXB27_001188"
     CDS             719..1711
                     /locus_tag="PXB27_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5345986.1"
                     /translation="MLCWALLLDVQDLRVTFKTPDGDVTAVNDLTFSLNAGETLGIVG
                     ESGSGKSQTAFALMGLLADNGRISGSAKFNGQEILNLPETQLNRLRAEQIAMIFQDPM
                     TSLNPYMKIGEQLMEVLMLHKGMDHKTAFEESVRMLDAVKMPESRKRMNMYPHEFSGG
                     MRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKSEFNTAIIMITHDLG
                     VVAGICDNVLVMYVGRTMEYGTAEQIFYTPSHPYSIGLIGAVPRVDGQEEELITIPGN
                     PPNLLRLPSGCPFQPRCVHATKQCLKAPALTLLNDQRLRACFVPAEQLIRGNAS"
     gene            1708..2697
                     /gene="oppF"
                     /locus_tag="PXB27_001189"
     CDS             1708..2697
                     /gene="oppF"
                     /locus_tag="PXB27_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HDL5345987.1"
                     /translation="MNTEKKVILEVKDLSVHFNIKDTKSLFFAKHQTLKAVNHVSFQL
                     YEGETLGVVGESGCGKSTLARAIIGLVESSGGNILWLGKELTQQSAKEWKTIRSDIQM
                     IFQDPLASLNPRMTIGSIIAEPLKIYQPHLTAEEVKEKVRAMMMKVGLLPNLINRYPH
                     EFSGGQCQRIGIARALIVEPKLIICDEPVSALDVSIQAQVVNLLKSLQREMGLSLIFI
                     AHDLAVVKHISDRVLVMYLGNAVELGDYEQVYHDTKHPYTKALMSAVPIPDPQLERNK
                     QIQLLEGDLPSPINPPSGCVFRTRCPLADQHCIADKPLLKGRDNHVVACFKAE"
     gene            complement(2801..3559)
                     /locus_tag="PXB27_001190"
                     /pseudo
     CDS             complement(2801..3559)
                     /locus_tag="PXB27_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014326384.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
     gene            complement(3689..4330)
                     /gene="bioD"
                     /locus_tag="PXB27_001191"
     CDS             complement(3689..4330)
                     /gene="bioD"
                     /locus_tag="PXB27_001191"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005724996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HDL5345988.1"
                     /translation="MAKVFFITGIDTDIGKTIATGWYAKKLMQQGASVITQKMIQTGC
                     RGIAEDLLIHRKIQGIELTEEDKQGITCPYVFDYPCSPHLAAKLEPRTIERKKIETCT
                     ALLCEKYDYVLLEGAGGLCVPYNEEETTLDYLCQHQYPVILVTSGKLGSINHTLLSLQ
                     VLNSKRVSVHAVIYNLYPETDQVISQETQHFLRRYLEKYSPNTLFEIMDLISV"
     gene            complement(4348..5103)
                     /gene="bioC"
                     /locus_tag="PXB27_001192"
     CDS             complement(4348..5103)
                     /gene="bioC"
                     /locus_tag="PXB27_001192"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005755527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HDL5345989.1"
                     /translation="MDRIDKGRIKQRFSAAVSNYDQQARAQQQIHQRLLSLLSYIDRQ
                     AFQRALEIGCGTGGLTKQLMSQLQVEHWELNDLCDMREHLQSVLPQPFHFHCGDAEQL
                     PFPQQYDLIASASVVQWFEDKAGFIARCKVRLKKHGFLLFSTFTPENLTQIKHLTGVG
                     LTYPRLEDWQKWLVPEFEILALEHEEIVLHFDDPLAVLKHLKQTGVTATQQQRWTKGQ
                     LHAFCQQYQQHYRNAQHKVELTYTPMYVLARLT"
     gene            complement(5107..5754)
                     /locus_tag="PXB27_001193"
     CDS             complement(5107..5754)
                     /locus_tag="PXB27_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005758126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF452 family protein"
                     /protein_id="HDL5345990.1"
                     /translation="MKTQHISQQGDNLIVYFAGWGTPPSVVEHLILPENYDLCLCYDY
                     RDLHCDVDFSAYQHIRVVAWSMGVWVAERVMQGYPLLSATAINGTGLPCDDQYGIPEA
                     IFAGTLSGLNEDNRTKFERRICGSKTLLRDYQHYSMRPTLQEIHAELTALYTFLQQDH
                     RTDLISWSKAIVGSKDKIFPAQNQRAYWQDRCAITEIAGEHLLFPHFTHWETCWL"
     gene            complement(5751..6914)
                     /gene="bioF"
                     /locus_tag="PXB27_001194"
     CDS             complement(5751..6914)
                     /gene="bioF"
                     /locus_tag="PXB27_001194"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005719374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HDL5345991.1"
                     /translation="MIRYYQQQLADLKAQNQFRQLPQLIHRGRFIQREDNTMLNMSSN
                     DYLGLANNEALRQAFFTQYQDQLPALTSSSSRLLTGSFPIYDELESLMAQAFGRETAL
                     LFNSGYHANIGILPALADKKTLIVADKLVHASMIDGIRLAQCEFVRFRHHDYVHLEQI
                     LQKNDRTFERIIVVTESVFSMDGDCADLTQLVALKQRYPQVMLYVDEAHAIGVLGEKG
                     LGLAEQQGCINQIDILVGTFGKALGSMGAYVICDQVIRDYLVNKMRPLIFSTALPPFN
                     VAWTHFVFQQLPHLQAERAHLAQLSQHLRQAIVDIFQVPMPSESCIVPYILGDNELTV
                     RTAQRLQQQGYYCLPIRPPTVPKGTSRIRFSLTADMQVAEVEQFIACLRELAE"
     gene            complement(6911..8212)
                     /locus_tag="PXB27_001195"
     CDS             complement(6911..8212)
                     /locus_tag="PXB27_001195"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016533607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HDL5345992.1"
                     /translation="MNEQQLLECDRQHIWHPYAALNADFPLFAVERAEGVYIQLKNGQ
                     QLIDGMSSWWSAIHGYNHPRLNHAATQQLSQMSHVMFGGLTHQPAVELAQQLVNILPE
                     SLDKIFFADSGSVAVEVAMKMAVQYQHAKGETQRYKFATIRSGYHGDTWHAMSVCDPV
                     TGMHSLFAKGLPVQYFLPQPPVKFGQAWQPEAMQPLQDLLAQHGDEIAGLILEPVVQG
                     AGGMYFYSPEYLVQAKALCEPYGILLIFDEIATGFGRTGKLFACEHANVVPDVMCIGK
                     ALTGGYLTLSATITNQAVADVICSGEAKCFMHGPTFMANPLACAVAAESIKLLLASPW
                     QQRIQQIEQQLRQELRQAEALDCVAEMRVLGAIGVIEMKAPVNMASLPPRFVQNGVWV
                     RPFGKLVYLMPPYIISSDQLSQLIHGTIRTLQEEYAHKESL"
     gene            8354..9241
                     /gene="psd"
                     /locus_tag="PXB27_001196"
     CDS             8354..9241
                     /gene="psd"
                     /locus_tag="PXB27_001196"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005755522.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HDL5345993.1"
                     /translation="MNILEKKAQLSYWQRIKIAFQYVMPQLYLTRLAGWFAKQQWGAV
                     THFVIKLFAKKYHVDMSEAAKPNFSDYASFNEFFIRPLADNARPINQNPTALCLPADG
                     RISQLGHIEQDLLLQAKGHYFSLNDLLAGDEALAHHFKDGEFATTYLSPRDYHRVHMP
                     CDATLCKMIYVPGDLFSVNPFLAEHVPNLFARNERVICVFDTEFGKMVQILVGATITA
                     SMSTVWAGVINPPRPEKITTWTYEGESAVKLLKGQEMGAFQLGSTVINLFEKDRVQLA
                     SHLQVDSPVRMGEILAHQK"
     gene            9266..9820
                     /locus_tag="PXB27_001197"
     CDS             9266..9820
                     /locus_tag="PXB27_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL5345994.1"
                     /translation="MSQQFFDQVSLESQLEVSAEAVAKGIFDVLNQNPPALELNEVSH
                     ALQTLQQQAKFKEIEAVGAQFLAENAQKAGVNTTESGLQYEVLVAGEGQIPAREDKVR
                     VHYTGTLIDGTVFDSSVKRGQPAEFPVNGVIAGWIEALSMMPVGSKWRLTIPHNLAYG
                     ERGAGASIPPFSTLVFEVELLAIL"
     gene            complement(9880..10203)
                     /locus_tag="PXB27_001198"
     CDS             complement(9880..10203)
                     /locus_tag="PXB27_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005755520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345995.1"
                     /translation="MNWKAVLIFSVLGSLVSCASYSEHAVQRIEAGKSFAVTGNTKRI
                     NTMACQDNDDWYLDGYRVGKSFREHQQKMLIQRTAYCEEQTGKAVPDKFRHSWNSGYQ
                     QGLKR"
     gene            complement(10227..11330)
                     /gene="proB"
                     /locus_tag="PXB27_001199"
     CDS             complement(10227..11330)
                     /gene="proB"
                     /locus_tag="PXB27_001199"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005719361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDL5345996.1"
                     /translation="MKIDKTLVVKFGTSTLTQGSAKLNLPHMIDIVRQLAQLHQAGFR
                     LVIVTSGAIAAGRHYLNHPQLPPTIASKQLLAAVGQSQLIQTWEKLFAIYDIHIGQIL
                     LTRADIEDRERFLNARDTLRALLDNQIIPVINENDAVATSEIKVGDNDNLSALVAILV
                     QAEQLYLLTDQQGLFDRDPRKHPDAKLIADVDKITDHIRAIAGGSGTSLGTGGMSTKI
                     SAADVATRSGIETIIAPGNRPNVIVDLAYGQAIGTKFSVQADRLESRKQWLYAAPSAG
                     IITIDAGAENAMLIQHKSLLPAGIVNVEGRFSRGEVVKIRTQQGKDVALGMPRYNSDA
                     LHLIQGKHSQDIEQVLGYEYGAVAVHRDDMIVL"
     gene            11496..12484
                     /locus_tag="PXB27_001200"
                     /pseudo
     CDS             11496..12484
                     /locus_tag="PXB27_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252470.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
     gene            12559..13206
                     /locus_tag="PXB27_001201"
     CDS             12559..13206
                     /locus_tag="PXB27_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HDL5345997.1"
                     /translation="MTINTYAKFAPTVFVAKCSESHTKGEIITLTSKYGNETEVQVHN
                     LVKQDGEHYFYSFTRCDGMDSQSFAQQKAERYQGYADNAAKRSQDWVNAANEGCDFLS
                     LGEPIKIGHHSEKRHRNLIERNARRMDNAVAEMNKAASYESRIAYWEKMAGKIDLSMP
                     ESLEYFKFELDKAKTKHKELKDNPEKREHGYSLTYAKKAVNELEKKVKLAELLWA"
     gene            13230..13637
                     /locus_tag="PXB27_001202"
     CDS             13230..13637
                     /locus_tag="PXB27_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization protein"
                     /protein_id="HDL5345998.1"
                     /translation="MNRQGIYINGKQVFDSQGEDIQSIGFFGCNIQTVVGINKGSILV
                     SSNSNQLLVNGQSIELGSEPVLKIEVLGNVQKLSAGAADVSITGDAGSVDTVSGDVSI
                     KGNVASYVKTVSGDVRCRAITGSVETVSGDIYR"
     gene            13735..14085
                     /locus_tag="PXB27_001203"
     CDS             13735..14085
                     /locus_tag="PXB27_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5345999.1"
                     /translation="MTTINLSQQERELVEFVWNNRNTPWFFHCSVSNVSRNNTSRQIA
                     VCVIKDNQLISLDELIAKLCGYKLRQNGLYVTVHNMDAVFDTLYRFMCKLNDEMEANE
                     NVCPYLSLAHYEVF"
     gene            14176..14538
                     /locus_tag="PXB27_001204"
     CDS             14176..14538
                     /locus_tag="PXB27_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346000.1"
                     /translation="MIDTFFDLDDTSCYLGNWEDILKEYDDCLSVEDFWHSAKEFDTV
                     PHFGNLYQERVILKLIARFCIELNIEQDSGLVEFDYYINAIDTHFYINSQRICDIDDW
                     NEMLDKIREEMTPAKLVA"
     gene            14651..14911
                     /locus_tag="PXB27_001205"
     CDS             14651..14911
                     /locus_tag="PXB27_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346001.1"
                     /translation="MTFELGRVCATRAVAECFNQHTLVDLLHRYCSGDWGNLCDNDKK
                     ANVKAIERGGRILARYDVEDETLYVITEADRSYTTILFPYEY"
BASE COUNT         4315 a         3229 c         3093 g         4297 t
ORIGIN      
        1 ctcaatttaa gacgctgtat ataacagcac ttaataagtc gcataactcc tgagaagcga
       61 cggctatcct tagaggtagt tgagttattg ccaaaatgtc tcctagcatt ctgaaaggga
      121 acgccacttt tgtgggaatg ccacctaaac cctctaccat ctcagtgaag catcattgag
      181 atttcatttt acttccacct cgcggggaac atttttcccg tgttgggcaa ggatgtttct
      241 ccgctttttt acatttggag aaacaaacta tgacaactca aacttctact caaaaaaatt
      301 acttcaacct tcatacaaca ggcattggct atctaaacga tattcgtttg gtagagccta
      361 aaaaaggaaa tcctttttat gcctgccgta tagcagctct ggttggcgaa tctgactctc
      421 ctgagtacag atattttgat atgaatgtag tcggtaaaga gactgaaaca cttatcaaaa
      481 gatgccaaaa agcagttagt gaagagaaaa aagtgcttgt gtcatttgta atggctgatt
      541 tgtggttcga tacatttact tactcaacag atagtaaatt ccacaaaaaa ggcgataccg
      601 gtgtatctgt aaaaggtcgc ttaatccgca tcaaaatgat taaaattgat ggggaaatag
      661 cctaccaaga aaagccaaaa tcagatgagc aatctgagta attttaccta agcccgacat
      721 tttatgttgg gctttattac tcgatgtaca agatttgcgc gtaactttta agaccccaga
      781 tggcgatgtt actgccgtca atgatctgac tttttcttta aatgccggtg aaaccctcgg
      841 cattgtcggc gaatctggct caggaaaatc gcaaaccgct tttgctctaa tggggctatt
      901 agcagataac gggcgaattt caggatcagc aaaatttaat ggacaagaaa ttctcaactt
      961 gcctgaaaca caacttaacc gcttgcgtgc tgaacaaatt gcgatgatct tccaagatcc
     1021 aatgacctcg ctgaaccctt atatgaaaat cggcgaacag ctaatggaag tactgatgtt
     1081 gcataaaggc atggatcata aaactgcgtt tgaagaatcg gtacggatgt tagatgcggt
     1141 gaaaatgccg gagtcacgta aacgtatgaa tatgtatccg catgaatttt ccggcggtat
     1201 gcgtcagcgt gtgatgattg cgatggcatt actttgccgt ccgaaactcc tcattgcgga
     1261 tgaacccaca actgcgctcg atgtgacggt gcaagctcaa atcatgacct tattaaacga
     1321 attaaaaagc gagtttaata ccgccattat catgattact cacgatctcg gtgtggtcgc
     1381 aggaatttgc gataatgtgt tggtgatgta tgtaggcaga accatggaat acggcaccgc
     1441 tgagcaaatt ttctataccc caagccaccc ttattctatc ggcttaattg gtgcagtgcc
     1501 tcgcgttgac ggacaggaag aagaactcat tactattccg ggtaatccac ctaacttatt
     1561 acgcttaccg agtggctgtc ctttccaacc acgttgcgtt catgcaacta aacagtgcct
     1621 caaagcaccg gcgttaacct tgttaaatga tcaacgttta cgcgcttgtt ttgtccctgc
     1681 cgagcaatta atccgaggta acgcatcatg aacacagaga aaaaagtgat cctagaagtg
     1741 aaggatttaa gcgttcattt caacatcaaa gatacgaaat ctttattctt tgctaaacac
     1801 caaactttaa aggccgtcaa tcatgtctct tttcagttat acgaaggcga aaccttaggt
     1861 gtggtcggtg aatcgggctg tggaaaatca actctcgcac gcgcgattat tggcttagtg
     1921 gaaagctctg gcggtaatat tttatggcta ggtaaggagt taactcaaca aagtgcgaaa
     1981 gaatggaaaa cgatccgtag cgatattcaa atgattttcc aagatccttt agcctccctg
     2041 aatccacgga tgaccattgg cagtatcatt gccgaaccat taaaaattta ccaaccccat
     2101 ttaaccgcgg aagaagtgaa agaaaaagta cgcgcgatga tgatgaaagt gggcttatta
     2161 cctaacttaa tcaatcgcta tccacatgaa ttttcgggtg gacaatgtca gcgtatcggg
     2221 attgcgcgcg ccttgattgt ggaaccaaaa ctcattattt gcgatgagcc agtgtccgcc
     2281 ttagatgttt caatccaagc acaagtagtg aatttattga aatctttaca acgcgaaatg
     2341 gggctatcat tgattttcat tgcacacgac cttgctgtgg tgaaacacat ttccgatcgt
     2401 gttttggtca tgtatcttgg taacgcggtg gaattaggtg actatgaaca agtctatcac
     2461 gatactaagc atccttatac caaagctttg atgtctgcgg taccgattcc tgatccgcaa
     2521 ttagaacgca ataaacaaat tcaattgctt gaaggcgatc tcccttcccc gattaatccg
     2581 ccgtcgggct gtgtgttccg cacccgttgc ccactggcag atcaacactg tattgcagac
     2641 aaaccgctcc tcaaaggacg agataatcac gttgttgctt gttttaaagc cgagtaatac
     2701 acacaaccgc acaaaaaagt gcggttgttt ttttgcgaat attcacagtc agactttcgt
     2761 gatactttcg ctctgagata aggattacga tacagagaag ttattcaaca atcacacgtg
     2821 cattgatcgg ttgcactgga cgattattgt gtcctttcat caaggtcgca aacgcctcaa
     2881 cttgagattg gctaatgttg tcatagtgtt ttaacactaa ccaatttaca ccttcagaac
     2941 aaggtggtgt agttaatgac ccgctgaaac gatagtaatc gcgttttttc ggaattaatg
     3001 ttgctgggtc aaaagcggta ctaagcgtat atttctcccc tgctttttgc gggaagtttt
     3061 gccaaattgg ggctaaccat tggttttcac ttgataatac atagagcacg ccaagcacag
     3121 caagattccc ttcattgtct gcgtgtacaa aatgtgcttc catcgggaag gatttacctc
     3181 aaattaagtt ttcactcggc acatggaaat ggaattgttt aagggtaaaa gtctttccat
     3241 taagctgcat gcgattactt gcctctttga aatccacttg aatagtatgc ccattattga
     3301 caatctccac attggaaggt cgataatcca gcttaatcgg cgctaactta ccatcaatcg
     3361 tatgcttgat atctattggt gtttggtttt taccttcgcc acaaatacgg aatttgggtg
     3421 aaagttcagc ccaatgttct ggcgattcgt gtccggtgta tccccaatgc gtgtgcgttt
     3481 cttctttctt aagggggctt gtacacgctg tcaatgctaa tgcacctaac gtaagcacaa
     3541 aaaggtttga ttttttcata gcctagctcc tgattaaaag agatgaattg atgagacaac
     3601 ctaaactcaa tgatgcctta atccgagacg ctttcatctt aggcaaaaca acactccatt
     3661 tcaagtataa cttgctcaag aatatatctt agaccgaaat caaatccatt atttcaaata
     3721 aggtattagg cgagtatttc tctaaataac gacgtaaaaa atgttgtgtt tcttggctaa
     3781 tgacttgatc tgtttccgga tacaagttgt atatcacagc atgcacactt actcttttgg
     3841 agttgagtac ttgtaaactc aataaggtat gattaatact acctagcttg ccagaggtaa
     3901 ctaaaatcac ggggtattga tgctgacaaa gataatcaag ggtggtttct tcctcattat
     3961 aaggtacaca caagccgccc gccccttcca ataatacata atcatatttt tcacacaaaa
     4021 gtgcggtgca ggtttcaatt tttttacgtt cgatcgtgcg cggttctaat ttcgcggcta
     4081 aatgcggtga gcaaggataa tcaaacacgt aaggacaagt gataccttgc ttatcttctt
     4141 ctgtaagctc aataccttgt atttttcggt gtatgagtaa atcttcggca attcctcggc
     4201 agcctgtttg gatcatcttt tgcgtgatga cacttgcgcc ttgttgcatc agtttcttgg
     4261 cgtaccaacc tgtggcgatg gttttgccaa tatcggtgtc gatgccagtg ataaaaaaaa
     4321 cctttgccat actttttcct tctaagatta agttaaacgc gccagcacat acatcggcgt
     4381 gtaagtgagt tctactttgt gttgtgcgtt acggtaatgt tgttgatatt gttggcaaaa
     4441 ggcgtgcaat tgcccttttg tccacctttg ctgctgtgtg gcagtaacgc ctgtttgttt
     4501 caaatgtttt aacaccgcta agggatcatc aaagtgcaac acaatctcct cgtgttcgag
     4561 ggctaaaatc tcaaattcag gcactagcca cttttgccag tcctcaagac gtggataagt
     4621 taaccccaca ccggtgaggt gtttgatctg tgttaaattc tcgggcgtga aagtactgaa
     4681 taacaaaaat ccgtgctttt ttaagcgcac tttacaacgc gcaataaaac ccgctttatc
     4741 ttcaaaccat tgcaccacgg aagcactggc aatcaaatca tactgctgcg gaaagggaag
     4801 ttgttccgca tcgccacaat ggaagtgaaa aggttggggc aacactgatt gcagatgctc
     4861 tcgcatatcg caaaggtcat tcagctccca gtgttcaacc tgcaactgtg acattaattg
     4921 cttcgttaat ccaccagtac cacatccaat ctctaatgca cgttggaatg cttgtctgtc
     4981 gatatacgac aataaactaa gcaaacgttg gtgaatctgc tgttgtgctc tcgcttgctg
     5041 atcatagttc gatacagcgg cagaaaaacg ctgtttaatg cgtcctttat caatacgatc
     5101 catgtgctac aaccaacaag tttcccaatg ggtgaagtga ggaaacaaca aatgctcccc
     5161 tgcgatttct gttattgcac aacgatcctg ccaataagcc cgctgatttt gtgcagggaa
     5221 aattttatct tttgagccga caatcgcctt gctccacgag attaaatctg tacggtgatc
     5281 ttgttgtaaa aaggtataaa gtgcggtcag ttcagcatga atttcctgta atgtgggacg
     5341 catggaatag tgctgataat ctcttaacag cgtttttgaa ccacaaatac gacgttcaaa
     5401 tttagtccga ttatcttcat ttaaaccaga taacgtgcca gcaaaaatgg cttcgggaat
     5461 accatattgg tcatcacaag gtaagcccgt gccattgatc gccgtagcag atagcagtgg
     5521 ataaccttgc atcacacgct ccgctaccca cacgcccatt gaccatgcca ccacgcgaat
     5581 atgttgataa gcggaaaaat caacatcgca atgcagatct cgataatcat agcaaaggca
     5641 taaatcataa ttttccggca gaatcaggtg ctcgaccacg ctcggcggtg tgccccaccc
     5701 tgcaaaatag acgatcagat tgtcgccttg ctgtgaaata tgttgtgttt tcattcagca
     5761 agctcccgta aacaagcaat aaattgttcc acttccgcca cttgcatatc tgcagtcagt
     5821 gaaaaacgaa tacgagaggt tccttttggg actgtcggcg ggcgaatcgg taagcaataa
     5881 tagccttgtt gttgcaaacg ttgcgccgtt cttactgtca gttcgttatc gcccaaaata
     5941 taaggtacga tacaactttc acttggcatc gggacttgaa aaatgtcaac aatcgcttgg
     6001 cgtaaatgtt gactaagctg agcaagatgt gcgcgttcgg cttgcaaatg tggtaattgt
     6061 tgaaagacaa aatgcgtcca tgccacatta aacggcggaa gtgccgtgga aaaaatcaat
     6121 gggcgcattt tattaaccaa ataatctcgg atcacttgat cgcaaatcac ataagccccc
     6181 atagagccca acgccttgcc aaacgtaccg actaaaatat caatttggtt gatacagcct
     6241 tgttgttctg ctaaccctag ccccttctcc cccaatacgc ctatcgcatg ggcttcatct
     6301 acatataaca tcacttgcgg atagcgctgt tttaatgcga ccaactgggt gaggtctgca
     6361 cagtcaccat ccatactaaa cacggattcc gtgaccacga taatccgctc aaaagtgcga
     6421 tcattttttt gcaaaatttg ttcaaggtgg acgtaatcat gatgacgaaa acgcacaaac
     6481 tcacattgtg ccaagcgaat gccatcaatc atactggcat gtaccagttt atccgccaca
     6541 atcagcgttt ttttgtccgc taaagcaggc aaaatcccaa tattagcatg ataaccgcta
     6601 ttaaatagca aggcagtttc tcgtccaaat gcctgcgcca ttaagctttc tagctcatca
     6661 taaatcggaa aagagcctgt taataaacgg gaggaagaag aagtcaatgc agggagttga
     6721 tcttggtatt gcgtgaaaaa tgcctgacgc aaggcttcgt tattggctaa acctaaataa
     6781 tcattggagg acatattcag catcgtattg tcctcacgtt gaataaaacg ccctcggtga
     6841 attagttggg gcagttggcg aaattgattc tgcgccttta aatcagctaa ctgctgctga
     6901 taatacctga tcatagagat tccttatgtg catattcttc ttgaagggtg cgaattgtgc
     6961 catgaattaa ttgagacaat tgatccgatg agatgatata cggtggcatc aaatacacta
     7021 atttgccaaa tggacgcacc caaacgccat tttgcacaaa gcgtggtggt aaacttgcca
     7081 tattgacagg cgccttcatt tcaatcaccc caatcgctcc aagtactctc atttcagcga
     7141 cacaatctaa ggcttccgct tggcgcaatt cttgacgtaa ttgctgttca atctgttgaa
     7201 tacgttgttg ccaagggctt gccaataaca atttgatact ttctgctgct accgcacacg
     7261 ccagtggatt tgccataaac gtcggaccgt gcataaaaca ttttgcctcg ccagaacaaa
     7321 tcacgtctgc taccgcttgg ttggtaatgg tagcggatag agttaaatac ccccccgtta
     7381 aagctttacc gatacacatc acatccggca cgacattggc atgttcacaa gcaaataatt
     7441 tgcctgtacg cccaaatcct gtggcaattt catcaaaaat cagcaaaatg ccgtacggtt
     7501 cgcataaggc ttttgcttgc accaaatact caggggaata aaaatacata ccacccgccc
     7561 cttgcactac cggttctaaa attaaacccg caatctcatc accatgttgt gccaataaat
     7621 cctgtaacgg ttgcatcgct tcgggttgcc aagcttgccc aaattttacc gggggttgcg
     7681 gtaaaaaata ttgcacgggt agccctttgg caaacaagct gtgcatgccg gtcacgggat
     7741 cacacacgga catcgcatgc caagtatcac catgatagcc cgaacgaatc gtagcaaatt
     7801 tatagcgctg tgtttcgcct ttagcatgtt gatattgcac tgccattttc atcgccactt
     7861 ccacggcgac cgaaccacta tcggcaaaga aaattttatc caaactttcc ggcaagatgt
     7921 tgaccagttg ctgcgccagt tctaccgctg gttggtgcgt taatccacca aacatcacat
     7981 gactcatttg actcagttgt tgcgtggcag cgtggtttaa acgggggtga ttatagccat
     8041 gaatcgctga ccaccacgat gacatgccat caatcaactg ctgaccgttt tttaactgga
     8101 tatacacacc ttcggcacgt tctactgcaa ataagggaaa atccgcattt aacgccgcat
     8161 aagggtgcca aatatgttgt cgatcacatt ctaaaagttg ctgctcattc atcgtttcac
     8221 ctcggttgtg aatcgccctt tcaactggcg taaattcacg caaatactta cataaacgga
     8281 ataacttgtt ataatcgcca agcatttggc ttttcaagcc cattattcta acttaaaaag
     8341 atgatttatt actatgaata tattagaaaa aaaagcacaa ctgagttact ggcaacggat
     8401 taaaattgcc tttcaatatg tgatgccaca actttattta acacgtcttg caggctggtt
     8461 tgcaaaacag caatggggtg ctgtcacaca ttttgtgatt aaattattcg cgaaaaaata
     8521 ccatgtggat atgagtgaag ctgcaaaacc aaatttcagc gactatgcga gctttaatga
     8581 attttttatc cgtccattag cggacaatgc ccgtccaatt aatcaaaatc ctaccgcact
     8641 ttgtttaccg gcagatggtc gtattagtca gttaggacac attgagcagg atttattgct
     8701 acaagccaaa ggacattatt tcagcttgaa cgatttgtta gctggcgacg aggcgctcgc
     8761 tcatcatttt aaagatggcg aatttgccac cacctacctc tcaccacgcg attaccaccg
     8821 tgtgcatatg ccttgtgatg ccaccttatg caaaatgatt tatgtgccag gtgacttatt
     8881 ctcagtcaat ccatttttag ccgaacacgt gccaaactta tttgcacgta atgaacgcgt
     8941 gatctgcgta ttcgataccg aatttggtaa aatggtccaa attttagtcg gcgcaaccat
     9001 taccgcgagt atgagcaccg tgtgggcagg tgtgatcaac ccgcctcgtc cagaaaaaat
     9061 caccacctgg acctatgaag gagaaagtgc ggtcaaatta ctgaaaggac aagaaatggg
     9121 agcctttcaa ttgggctcga cggtgatcaa tttatttgaa aaagatcgtg tgcaactggc
     9181 atctcattta caagtggata gcccggtacg tatgggtgaa attcttgccc accaaaaata
     9241 atttttaacc acaataggaa aaacaatgag tcaacaattt ttcgaccaag tgtcattaga
     9301 aagccaactt gaagtgagcg ccgaagccgt cgcgaaaggg atctttgatg tgttaaatca
     9361 aaatccacca gcacttgagt taaatgaagt gagtcatgcc ctacagactt tgcaacaaca
     9421 agcgaaattc aaagaaattg aagccgttgg tgcacaattc ttagctgaaa acgcgcaaaa
     9481 agcgggggta aatactacgg aatctggttt acagtatgaa gtgttagtgg ctggtgaagg
     9541 gcaaattccg gcacgcgaag acaaagtgcg tgtgcattac actgggacat taatcgacgg
     9601 taccgtcttt gacagctcag taaaacgtgg tcaacctgca gaattcccag taaatggcgt
     9661 gatcgctggt tggattgaag ccttgtccat gatgccagtg ggttcaaaat ggcgtttaac
     9721 cattcctcac aacctcgctt atggcgaacg tggtgctggt gcctccattc cgccattcag
     9781 taccttagtg tttgaagtcg aattattagc cattctttaa agctaaacgc tagacataaa
     9841 aaagtgcggt cattttccgc acttttttat gtctagcgtt tatcgtttca agccttgctg
     9901 atagccgctg ttccacgaat ggcggaactt gtccggcaca gccttgcccg tttgttcttc
     9961 acagtaagcc gttcgttgaa tcaacatttt ttgttggtgt tctcgaaatg attttcccac
    10021 acgatagccg tccaaatacc aatcatcatt atcttgacat gccatggtat taatccgttt
    10081 tgtattcccc gtcactgcaa aagatttgcc tgcctcaata cgttgcaccg catgttcaga
    10141 gtatgaagca cagctcacca gcgaacctag tacactgaat atcaatactg ctttccaatt
    10201 catcttgtta cctttctttt tctgatttat aacacaatca tatcgtctcg gtgtaccgca
    10261 accgccccat attcataacc gagaacctgt tcgatatctt gcgaatgttt gccttgaatt
    10321 aaatggagcg catcgctgtt ataacgtggc atccctaagg caacatcctt gccttgctga
    10381 gtacgaattt ttaccacttc accgcgtgaa aagcgccctt ccacgtttac aatcccagca
    10441 ggcaataacg atttatgttg tattaacatc gcgttttccg cccccgcatc aatggtaata
    10501 ataccggctg acggcgccgc atatagccat tgtttacgac tttctaagcg atctgcttgc
    10561 acactaaatt tggtgccaat ggcttgccca taagctaagt ccacaatcac attcgggcga
    10621 ttacccggtg caataatggt ttcaatgccg gagcgggttg ccacatccgc ggcactgatt
    10681 ttggtggaca tgccacctgt gcctaacgag gtgccacttc cgcccgcaat cgcgcgaata
    10741 tgatcggtaa ttttgtccac gtcggcgatc aatttggcgt ctggatgttt gcgcggatca
    10801 cgatcaaata aaccttgctg atcagtaagc aaatacaact gttctgcttg caccaaaatt
    10861 gccactaacg cggataaatt atcgttatcg cctactttaa tttctgacgt agcgacagcg
    10921 tcattttcat taatcactgg aataatctga ttgtctaata aggcgcgcaa agtatctctg
    10981 gcatttaaaa aacgttcacg atcttcaatg tcggcacggg tcaataaaat ctgcccaata
    11041 tgaatatcat aaatggcaaa caatttttcc cacgtttgaa tgagctgact ttgtcctacc
    11101 gcagccaata attgtttcga ggcaatcgtc ggtggcaatt gcgggtgatt taaataatga
    11161 cgacctgccg caatcgcccc tgaggtaaca atcactaaac gaaagcccgc ttgatgaagc
    11221 tgtgcgagct gacgcacaat atctatcata tgaggtaaat ttaacttcgc cgatccttgc
    11281 gtaagcgtac tggttccaaa ttttacaacg agtgttttgt cgattttcat cttctctatc
    11341 ttcttatttt tgtcgcacaa aattgaagct aacttaccat taatcatcag aaaaatcatc
    11401 tcgtttactt gctattttct atgatttagc acaaattttt tctcactaaa aggcatataa
    11461 taaagcttct tgatttttca gctaggaacc cgattatgac ctcaacctct tcacaagaaa
    11521 cggagcggca acgagaaatc actcgccttt gtgttcaagt tgcccaactc ttatacaaca
    11581 tggtgcagaa agtgccatcg ttgtgcaaat ggcacatcgc ttaggcttag ccctcggtgt
    11641 agatagcgta gaatgtgccc tcacgccaaa tgccattatt cttactacat tagcccaaga
    11701 ccattgcatc acgacagtcc gtaaaaacca agacaaagga attaatatgc aagtggttac
    11761 cgaagtgcaa cgtattgtga ttacagcaga acgtaaaatc tatgatatgc aacaagtgca
    11821 tgccaaactg gctcgcatta aaccgcttaa atacaatcgc tattttgtga tgttgatgat
    11881 tggcgtatcc tgtgccagtt ttgctcattt atctggtggg gacgcactag tttcattggt
    11941 tacatttctc gccgcaacac tcgccatgta tgttcgccaa tcacttgccg caagacacta
    12001 taacccctta attgtatttg cggtgaccgc ctttgtcgcc tcactaattg cgggtatcgc
    12061 gttgaaatat aacatcggca atcagcccca aattgccttg gcatccagcg tattattgct
    12121 cgttcccggc tttccactga ttaatgcact cgccgatatt ttaaaagggc atatcaatat
    12181 ggggattgcc cgttgggcac tcgcgacaat cctcacattt ggggcatgct taggcattgt
    12241 atttgcgctg agtttattct tagaggaaat ctgtatgcta aataaattca ttcaagatga
    12301 atttctacgg ctagatcaac gttatgcagc ttggtatcaa agtgaagaaa cggatgaagt
    12361 tgccagaatg gggattcaag aaagtgagcg gattttgaaa attctgtatg cactgaaata
    12421 taacgaagac cctatgcaaa atggtttagg ctatattgca tttatggcat caaaaggcca
    12481 ataatttact tatctcaaca atttacccac cggggaaact cctcgatggc gtttccccta
    12541 ctttatagga gaaacgctat gactattaac acttatgcaa aatttgcacc taccgttttt
    12601 gttgcaaaat gctctgaatc acataccaaa ggtgaaatta ttacgctaac cagcaaatat
    12661 ggtaatgaaa ccgaggttca agtacataat cttgttaaac aagatggcga gcattatttt
    12721 tattcattta ctcgttgtga tgggatggat agtcagtcat ttgctcaaca aaaagcagaa
    12781 cgctatcaag gctatgcaga taatgcagcc aaacgtagtc aagattgggt aaatgccgct
    12841 aatgaggggt gtgatttttt gagtttaggt gagccaataa aaattggtca tcacagcgaa
    12901 aagagacacc gtaaccttat tgaacgtaat gctcgccgta tggacaatgc ggttgcagaa
    12961 atgaacaaag ctgcaagtta tgaatctcgt atcgcatatt gggaaaaaat ggcaggaaaa
    13021 attgacctgt ctatgccgga aagtctggaa tactttaagt ttgaattgga taaagccaaa
    13081 acaaagcaca aagaattgaa ggacaacccc gagaaacgag agcatggtta ttcactgact
    13141 tatgctaaaa aagcagttaa tgaacttgag aaaaaagtaa aattagctga attactttgg
    13201 gcataattat ttacaaagga gatgacgata tgaatcgaca aggtatttat attaatggca
    13261 aacaggtgtt tgattcacaa ggcgaagata ttcaaagcat tggatttttt ggctgcaata
    13321 ttcaaacggt cgtaggcata aacaaaggaa gtattttagt ctcgtccaat agtaatcaat
    13381 tattggtgaa tggacagtcg attgaattag gttctgagcc agtcctcaaa attgaggttt
    13441 tgggcaatgt gcagaaactg agtgccggtg ccgcagatgt ctctattaca ggagatgccg
    13501 gcagtgttga cacagttagc ggtgatgtct ctattaaggg aaatgttgcc tcttatgtga
    13561 aaaccgtgag tggtgatgtt cgctgtcgtg ctattaccgg ttcggtggaa accgtctccg
    13621 gtgatattta tcgctgatat ttactcatct tattatttac caccctattg ggaagcaatt
    13681 ttcccaaggt gggcagattg tttcccattt ttattttgaa tttggagaaa catgatgacc
    13741 actattaacc tttcacaaca agaacgtgag ttagttgaat ttgtctggaa taaccgtaat
    13801 acaccttggt tttttcactg tagtgtatcg aatgtgtcga gaaataatac ctctcggcaa
    13861 attgctgtat gtgtcattaa ggataatcaa ttgattagcc ttgatgaatt gatagcaaaa
    13921 ttatgtggtt ataaactcag acaaaacgga ttatacgtta ctgttcataa tatggatgct
    13981 gtttttgata ccttatatcg ctttatgtgc aagctaaatg atgagatgga ggcaaatgaa
    14041 aatgtttgcc cttatttatc attagctcac tatgaagtgt tttgattggt atcagaacta
    14101 tccaccctgt cgggaaacaa tttttccgat agggcagatt gtttcccact tttattttga
    14161 atttggagaa acataatgat agacaccttt tttgaccttg acgacacctc atgctattta
    14221 ggtaactggg aggatatact gaaagaatat gatgattgtc tttcagttga agatttttgg
    14281 cattccgcta aagaatttga taccgtacct cattttggca acctatacca ggagcgtgtc
    14341 atccttaagt tgattgctcg attctgtatt gagctgaata ttgagcaaga ttcaggttta
    14401 gtcgaatttg attattatat caatgcaata gatacgcatt tctatattaa tagccaaaga
    14461 atctgtgata tagatgattg gaatgaaatg ttagataaga ttcgtgaaga aatgacgccg
    14521 gctaaattag ttgcctaatt tttgcaaatt tttactaaaa atgacgctta ctgtcgtctt
    14581 aattcaccca cttggggaaa tatctcccca atgggatgac atttcccctc tttctgttta
    14641 ggagaaatgt atgacttttg agcttggtag agtatgtgca actcgtgcag ttgctgaatg
    14701 tttcaatcaa catacattag ttgatctact tcatcgctac tgcagtggtg attggggaaa
    14761 tttgtgtgat aacgacaaga aagccaatgt gaaagctatt gaacgcggag gacgcatttt
    14821 agctcgctat gatgtggaag atgaaacact ttatgtaatc acagaggctg atcgtagtta
    14881 cactacaatt ttatttcctt atgaatatta atggagcttc catttatacc ctac
//
LOCUS       DANLUQ010000050        14785 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.050, whole genome shotgun sequence.
ACCESSION   DANLUQ010000050 DANLUQ010000000
VERSION     DANLUQ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14785
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.050"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..278)
                     /locus_tag="PXB27_001206"
     CDS             complement(<1..278)
                     /locus_tag="PXB27_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Type II secretory pathway, pseudopilin PulG"
                     /protein_id="HDL5346002.1"
                     /translation="MYKAITLIEILTTITIMVIAVYFISPVIFTLQDRIALHSEIENI
                     QSFIQQIQTKARYTKRNYTLTLSQRNNQWCMIAVIKPINTSKQIICDC"
     gene            complement(379..828)
                     /locus_tag="PXB27_001207"
     CDS             complement(379..828)
                     /locus_tag="PXB27_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudoazurin"
                     /protein_id="HDL5346003.1"
                     /translation="MLAKLKWNQLACVTLAFCSASVFSAHHEIKMLDNNAEGSMVFEP
                     GYVKAEVGDTVTFIPSHKGHWVESRSIPEGAEKFLSKEDEEFTITLTHEGVYVYYCPP
                     HRTMNMAGIIQVGQALNLEATQKEVEKIEKRTKENKGRLFEYLEQVK"
     gene            complement(888..1607)
                     /locus_tag="PXB27_001208"
                     /pseudo
     CDS             complement(888..1607)
                     /locus_tag="PXB27_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616566.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            1663..2313
                     /locus_tag="PXB27_001209"
                     /pseudo
     CDS             1663..2313
                     /locus_tag="PXB27_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021111752.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            2565..3755
                     /locus_tag="PXB27_001210"
     CDS             2565..3755
                     /locus_tag="PXB27_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HDL5346004.1"
                     /translation="MSTSQQLIQQTEHFGANNYLPLPIVISKAKGIWVEDPEGNRYLD
                     MLSAYSAVNQGHCHPKIVQALKDQADKVTLTSRAFYNDQLGIWYEKICKLANKEMALP
                     MNTGAEAVETAIKTARRWGYDVKGIAENQAEIIACVGNFHGRTMAAVSLSSDPDYKRG
                     FGPMLGGIKLVPYGDLEALKAAITPNTAAFLVEPIQGESGILIPPEGFLEQAYELCKE
                     NNVLFIADEIQAGLGRTGKLFATEWEGFEPDIYILGKALGGGVFPISCVLANRDILGV
                     FNPGSHGSTFGGNPLACAVSIASIDVLLEEKLAERSRELGEYFLAELKKIQNPIIKEV
                     RGRGLFIGLELHQPARPFCEKLKEKGLLCKETHDTVIRFAPPLVIEKFNLDWALEKIR
                     EVLA"
     gene            3830..5341
                     /gene="nhaC"
                     /locus_tag="PXB27_001211"
     CDS             3830..5341
                     /gene="nhaC"
                     /locus_tag="PXB27_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL5346005.1"
                     /translation="MLILQTFSKNQPLVKGGFMSNRTEITDQNIRIPFRYAILPVIIM
                     VSIMAVAIIKFEASPHVPLLIGAISAAVIAMAHGYRWETIEKGIYRGIRMALPAIVII
                     IMIGLTIGAWLGGGIVATMIYYGLKLISPSLFLVTISIICAIVTLAIGSSWSTMGTIG
                     VAAMGIGFSLGIPAPMVAGAVISGAYFGDKMSPLSDTTNLAAGITETNLFEHIRHMFI
                     TTVPAFLIALSVYFYFGMEFRAENLDLAKIEDIMQAMQEHFLISPWLLLVPLLVVVLV
                     AKKVPALPALAAGIILGWLCHVFVQGGDVSVAVITLHDGYKIESGHEMIDKLFNRGGI
                     ESMMYTISLTIVAMTFGGIAEQAGMLHSVVEKILTLAKSAGSLISATIVSAFITNVTT
                     SEQYISILLPGRMYIKAYRDKGLSSRNLSRALEDGGTVTSVLVPWNTCGVYAFSMLQV
                     SAFEYAPYAIMNWLIPLIAIGLATLNIKIERLPPEELAKEPEEITLPKRGCCP"
     gene            5458..9057
                     /gene="putA"
                     /locus_tag="PXB27_001212"
     CDS             5458..9057
                     /gene="putA"
                     /locus_tag="PXB27_001212"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HDL5346006.1"
                     /translation="MMSYQLLPNTTIRPELSRHYRFDEQTLVQNLIEQAHCDEQADNI
                     KKLTTQLVGKVRTARQKASGVDALMHEFSLSSQEGVALMCLAEALLRIPDTATADKLI
                     RDKIAKGDWRSHTGNSPSLFVNAAAWGLVITGKLVSTHSESSLSSALSRLIAKGGEPL
                     IRKGVDVAMRMLGKKFVTGETIQQAIKNGEKRFAMGYRYSYDMLGEAALTAEDAKRYY
                     NDYVASIHAVGATSKGLGVYKSSGVSVKLSAIHPRYSLSQHQRVMDELYPRLKELFLL
                     AKKYDIGLNIDAEEADRLELSLDLMDRLLADPDLAGFNGIGFVVQAYQKRCPYVIEHL
                     IETARKHNRQLMIRLVKGAYWDTEIKRAQMDGAEGYPVFSRKVHTDLNYIVCAKKLLA
                     AQDVIYPQFATHNAQTLATIYHLAQGKRFEFQCLHGMGESLYDQVVGSDNLNVQCRIY
                     APVGTYQTLLAYLVRRLLENGANSSFVSQIVDENLSVDDLAQAPVQKAELTNGTMHPK
                     IPLPVDLFDDNRVNSKGYDLTDAVSLQNLENALNELTKQHYRAEPQLANDNTSGQESR
                     KIYNPANQAELVGEVIDATENDMEQAFESAAAFQSEWANTSPAVRAECLEKMADLMEA
                     NMPQLFDLAVREAGKTLNNAIAEVREAVDFCRYYAKEVRNNIEAYKNPRGIVVAISPW
                     NFPLAIFVGEVSSALVAGNVVLAKPAEQTSLMAHYAVSLFYQAGVPKAALQCLPGRGK
                     TVGVALVADHRVNGVIFTGSTETAKNINRNLHKNEKIIPLIAETGGQNAMIVDSSALG
                     EQVVIDVLNSAFDSAGQRCSALRVLYLQRDVADHILTMLKGAMAELKVGLPQHLTTDV
                     GPVIDKVAQQRLLDHIENLKKSAKSYYQVAISSEIAENGIFVPPTLLEIDHISQLKHE
                     VFGPVLHVIRFDADKLDQVIADINSTGFGLTSGLHSRIDETVDLWLKSVHAGNLYVNR
                     NTVGAVVGVQPFGGMGLSGTGPKAGGPLYLQRLVNRQEWTLNGLQGNAQAVDFDLNFA
                     IRKVLSGAEQQTALDLAKSLARRSPLGKTYPMQGITGEKNEMIFVARPQIGISKGNLA
                     SQVQALIAIVTVGSQAVAEKSSELAKHASAFGKKLQVVDDFNSLDHLSTLIVLDLLSS
                     EEKLHHAERKGAILTYIEGLDLALSLLPLVHEKAISINTAAAGGNASLMSEMD"
     gene            9166..9816
                     /locus_tag="PXB27_001213"
     CDS             9166..9816
                     /locus_tag="PXB27_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595-like element ISMha3 family transposase"
                     /protein_id="HDL5346007.1"
                     /translation="MRKSRLSQYKQHKLIELFVAGVIARTAAELVNVNKTTAAYYFHR
                     LRLLIYQHSPHLEMFEGEIEIDESYFGGHRKGKRGRGAAGKTAVFGLLKRDGKVYTVV
                     VPNTQSATLLPIIREKVKPDSLVYTDFYRSYDVLDVSEFNHFRINHSTHFAENQNHIN
                     GIENFWSQAKRHLRKFNGIPKAHFELYLKECEWRFNHSDLKSQIFLLKQLVKGGLG"
     gene            complement(9817..10704)
                     /locus_tag="PXB27_001214"
     CDS             complement(9817..10704)
                     /locus_tag="PXB27_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide transport protein"
                     /protein_id="HDL5346008.1"
                     /translation="MNKLTQLALISLAFTGSAFAAPKSFTYCLESSPAALNPQIATDG
                     ATLDVGQAIYNRLIDFEKGTTNLTPSLAERWEISEGGKIYTFFLRQNVQFQSNKSFTP
                     SRYFNADDVIFSINRQFDKTNPYHKLSGGNYEFFYGMNMQNIIKNVTKVDDYTVKIEL
                     SEPNAPFLANLAMDFMAIFSAEYANKMQIAGTPEKLDLEPIGTGAFQFYGYQKDAAVR
                     FTAFEHYWEGKAKIDRLVFSITPDASVRLAKLEKNECQAMPYPNPSDLSALKANKDIQ
                     LFEKSGLNIGYITFRGCPR"
     misc_feature    complement(10730..10875)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            complement(11052..12062)
                     /gene="mglC"
                     /locus_tag="PXB27_001215"
     CDS             complement(11052..12062)
                     /gene="mglC"
                     /locus_tag="PXB27_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018355241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HDL5346009.1"
                     /translation="MSALQQNRTLDFFKQNAIYFVLLILLIVIIVQDPSFLSLRNFSN
                     ILSQSSVRLIIALGVAGLIVTQGTDLSAGRQVGLAAVISATLLQSMENMNKVFPNLSE
                     IPIPVVVLTVCAIGAVIGLFNGFVVAYLNVTPFIATMGTMIIVYGINSLYYDAVGSSP
                     IAGFSDDFSTFAQGFFRFGDFRLSYITIYAVIATIIMWTLWNKTRFGKNVFAIGGNPE
                     AARVSGVNVPLNLMGIYMLSGIFYAFGGMLEAGRIGSATNNLGFMYEMDAIAACVVGG
                     VSFAGGVGTIIGVVTGVLIFTVINYGLTYIGVNPYWQYIIKGGIIILAVAIDSLKYAK
                     KK"
     gene            complement(12076..13575)
                     /gene="mglA"
                     /locus_tag="PXB27_001216"
     CDS             complement(12076..13575)
                     /gene="mglA"
                     /locus_tag="PXB27_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005709009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HDL5346010.1"
                     /translation="MTGEVLLTMTNVSKSFPGVKALDNANLTVKSHSVHALMGENGAG
                     KSTLLKCLFGIYAKDEGDILFLGKPVNFKTSKEALENGISMVHQELNLVKQVTVMDNL
                     WLGRYPLKGPFVDHDKMYRDTKAIFDELDIDVDPRDKVAKLSVSQMQMIEIAKAFSYN
                     AKIVIMDEPTSSLSEKEVEHLFKIIQKLKDRGCGIIYISHKMDEIFKICDEITILRDG
                     KWVNTVAIKDTSMDQVVSMMVGRELTQRFPEKTNLPKEVILEVEHLTAKNQPSIQDVS
                     FSLRRGEILGIAGLVGAKRTDIVETIFGVREKAGGTIRLNDKEINNRNAFDAINNGFA
                     LVTEERRSTGIYANLSIEFNSLISNMKSYLGKFGLLSNKKMKSDTQWVIDSMNVKTPS
                     HQTHIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDIGAKFEIYQLILELANKDK
                     GIIMISSEMPELLGVTDRILVMSNGKVAGIVETAHTSQEEILQLAAKYL"
     gene            complement(13647..14636)
                     /gene="mglB"
                     /locus_tag="PXB27_001217"
     CDS             complement(13647..14636)
                     /gene="mglB"
                     /locus_tag="PXB27_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015701875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HDL5346011.1"
                     /translation="MKKAVLSAVAMAVALGATISTAQAENRIGVTIYKYDDNFMALMR
                     KEIEKEGKTQKVELLMNDSQNTQSIQNDQVDVLLSKGVKALAINLVDPSAYKTIIQKA
                     KSEDVPVVFFNKDPGAAAIDSYPKAYYVGTEPKESGIIQGELIAKQWKAHPELDLNKD
                     GKIQFALLKGEPGHPDAEARTKYVVESLKAQGIDSEQIFMDAALWDAAQAKDKVDAWL
                     SSGKAKDIEVIISNNDGMALGALEATKAHGRKLPIFGVDALPEALQLIKKGELAGTVL
                     NDGVNQGKAVVQLSANLADGKDAVAGTQWKLENRVVRIPYVGVDKDNLSEFLK"
BASE COUNT         4280 a         3080 c         3095 g         4330 t
ORIGIN      
        1 aaacaatcac aaataatttg tttgctagta ttgattggct taattaccgc aatcatacac
       61 cactgattat tccgctgaga aagggttaag gtataattac gtttggtata acgtgcttta
      121 gtttggattt gctgaataaa agattggata ttttcaattt cactatgaag tgcaattcga
      181 tcttgcagtg taaaaataac cggcgagata aaataaacag cgatcaccat aatagtgata
      241 gtggttagta tttcaattaa tgtgatggct ttatacataa aaataccctc attgatagag
      301 ggtatcttag aatttaagca tcatttgtaa actattaaat attgattttt cgatgcagat
      361 cgcaaaatta aatcacttct acttaacttg ctctaaatat tcaaataaac gtcctttatt
      421 ttctttcgtg cgtttttcaa ttttttccac ttctttttgt gtggcttcaa gattgagagc
      481 ttgtcctact tggataattc cggccatatt catggttcgg tgtggcggac agtagtaaac
      541 ataaacacct tcgtgagtaa gggtaatagt aaattcctca tcttctttag ataagaactt
      601 ctccgcacct tccggaatcg agcggctttc aacccaatgc cctttatgag acggaataaa
      661 tgttaccgta tcgcccacct ctgcttttac ataaccgggc tcaaatacca ttgatccttc
      721 cgcattgtta tctaacattt tgatttcgtg atgggctgaa aatactgagg ctgaacaaaa
      781 tgccaatgtt acacaagcta attgattcca cttaagtttc gctaacatat atttattgct
      841 cctaaattta tgaggcacaa aatgagttca attcattata ccttaggtta ttccagctca
      901 acaccataaa aattatgagc aataaacggg ctgacaacat agccttttac ctctttcctt
      961 accggaaaat acaccgttga atgtgcaatc ggtaaccaag gtgcttgctc attaaagatg
     1021 aattgcgctt ttttatataa atctgaccgc ttgttttgat cactgatagt cgcagcctca
     1081 ttaattaaac ggtcaaactc tggattacaa aaccctgcat agtttgagcc ttgttctacc
     1141 gcattacaac tgagtagggc attgaggaaa ttatctggat cgccgttatc tccggtccaa
     1201 ccaatcattc cggtagcgtg ctcgccgttt cccattcgtt taagatattc tccccattca
     1261 tagctcacta tctttgcctc tacccctatt gctttccaat cctcttgaat catttcagcc
     1321 attctacggg catttggatt gtaagggcga gaaacaggca ttgcccaaat ttctgtttca
     1381 aagccatttt ctaatccggc ctctttaagc aagctcttcg ccttttccgg attaaattca
     1441 taaccggcta cttcgttgtt atatccccaa atcgtagaag gcattgggtt tttagctggc
     1501 gttgcagagc cttggtaaac ggaagttaaa atttcagttt tattcaccgc ataattcaat
     1561 gcttgacgaa ctttagggtt atcaaaaggg ggcttacgag tattaaaagt aggggctgtc
     1621 ctagataacg actaaaactt ccatttaggt taagataacc caatgagaaa aagtcgtcta
     1681 agtcagtaca aacaacataa actcattgaa ctgtttgtag caggtgtcac tgctcgaaca
     1741 gcggctgagt tggtgaatgt aaacaaaacc accgctgcct attactttca ccgtttgcga
     1801 ttgctcatct atcaacatag ccctcatctg gaaatgtttg agggtgaaat tgaagcggac
     1861 gaaagttatt tcggtggtgc tcgcaaaggt aaacgagggt gtggtgccgc aggcaaaact
     1921 gccgtatttg ggctgttaaa acgtgatgac aaggtttata ccgttgtcgt accaaatacg
     1981 caatctgcca cacttttgcc gattattcgg gagaaagtga agccagacag ccttgtttac
     2041 gccgattttt atcgaagcta tgatgtgctt gatgtgagtg aatttaacca ttttcgtatc
     2101 aatcacagca cacacttcgc tgaaaatcac aatcacatta acagaattga gaatttttgg
     2161 agccaagcaa aacgccattt gcgtaaattt aacggcatac ctaaagctca ttttgagctg
     2221 tatttgaagg aatgcgaata gcgttttaat cacagtgact taaaatctca aatttccatt
     2281 ttaaaacaat tggttagggg ttgtttaagt tagttatcta ggacagccct aaattttgta
     2341 gttggaattt caacggagaa agttatctat aaacttacaa aagttataga aaatggaggc
     2401 atttttgctt aaagtatgct atctattcat attaattgca tagttaatca acaactgaag
     2461 aaaaattaga agtagatcat aaaatcaaca aaaagtccgt gctttttagc taaagattgt
     2521 taaactcttt tgtgttacaa gtttttaaca taaaggagtt ttttatgtct acttctcaac
     2581 aactcatcca acaaaccgaa catttcggtg ccaataacta cctacctcta cctattgtta
     2641 ttagtaaagc taaaggcatt tgggttgaag atcctgaggg taatcgctat ttggatatgc
     2701 ttagtgccta ctcagcggtt aatcaagggc attgtcatcc aaaaattgta caggcactaa
     2761 aagatcaagc agacaaagta acgctgacct ctcgagcatt ttataacgat caacttggta
     2821 tttggtatga aaaaatctgc aaactcgcta ataaagagat ggcattaccg atgaatacgg
     2881 gtgcagaagc ggtagaaaca gcgattaaaa ctgctcgccg ttggggttat gatgtgaaag
     2941 gaattgcaga aaatcaagca gaaatcatcg cttgtgttgg caattttcac ggacgaacaa
     3001 tggctgcagt atctctctcg tctgatccgg attataaacg tggtttcggg ccgatgttag
     3061 gtggcatcaa acttgttcca tacggtgatt tagaagcatt aaaagctgcg attacaccaa
     3121 acacagcagc gtttttggtt gagccaatcc aaggtgaatc tggcattctt attccacctg
     3181 aaggtttctt ggaacaggct tatgaactat gcaaagagaa taatgtgctg tttattgctg
     3241 atgaaattca ggcaggtctt gggcgtactg gcaagttatt tgcaacagaa tgggaaggct
     3301 ttgagccaga tatttatatt ctaggtaaag cacttggtgg cggggtgttc ccaatctctt
     3361 gtgtgttagc aaatcgtgat attttaggcg tattcaatcc tggctcgcac ggttcgacat
     3421 ttggtggtaa tccattagcc tgtgcggtat ctattgcctc aattgatgta ctgttggaag
     3481 aaaaacttgc agagcgttcc cgtgagttag gtgaatactt cttggcagag ctgaaaaaaa
     3541 tccaaaatcc aattattaaa gaagttcgtg gacgtggact gtttattggc ttggagttac
     3601 atcaaccagc acgcccattc tgcgaaaaac tcaaagaaaa aggcttactc tgcaaagaga
     3661 cccacgatac agttatccgc tttgctcccc ctttggtgat tgaaaaattc aatttggatt
     3721 gggcattgga gaaaattcgg gaggtgttgg cttaattgtc attttctcta tattttatcc
     3781 tgccaaatcg cctttaaccc tttttggcag gaagcaaaac agttgtaaaa tgctgatttt
     3841 gcaaactttt tccaaaaatc aaccgcttgt aaaaggtggc tttatgtcaa acagaacaga
     3901 aataacagac caaaatatcc gaatcccgtt tcgctatgct atattgcctg tgattattat
     3961 ggtatcgatt atggcggtgg ctatcatcaa atttgaagcc tcacctcacg ttccgttgct
     4021 tattggggca attagtgccg ctgtgattgc aatggcacat ggctaccgtt gggagacaat
     4081 tgaaaaagga atttatagag gcattcgtat ggctttacca gcgattgtta tcattattat
     4141 gattgggcta accatcggag cttggctagg tggtggaatt gtagctacga tgatttacta
     4201 cggcttgaaa ttgatctctc cgtcgctgtt tttagtaact atctcaataa tttgtgctat
     4261 tgtgaccctt gcgattggta gctcgtggtc tacaatggga acaatcggtg tagcggcgat
     4321 ggggatcggg tttagtctcg gcattccggc accgatggtt gccggtgcgg tgatctcagg
     4381 agcatatttc ggcgataaaa tgtcgccact gtcagacact accaatctgg cagcagggat
     4441 taccgaaacc aatctgtttg aacatatccg ccatatgttt atcactaccg tgccggcgtt
     4501 cctgattgcg ttatctgtct atttctattt tggtatggaa tttagagccg aaaatctcga
     4561 tcttgctaaa attgaagaca ttatgcaggc aatgcaggaa catttcttga tttcgccttg
     4621 gttgctatta gtgcctttat tagtcgtggt gttggtggca aaaaaagtgc ctgccttacc
     4681 ggcattagct gcagggataa ttcttggttg gctctgccac gtttttgtgc aaggcggtga
     4741 tgtcagcgta gcagtgatta ctctgcacga cggctataaa attgagagcg gccacgagat
     4801 gattgataag ctgttcaacc gtggtgggat cgagtcgatg atgtacacca tttcccttac
     4861 gattgttgcg atgacttttg gtggcattgc agagcaagct ggaatgttac attcagtagt
     4921 ggaaaaaatc cttacccttg caaaatcagc aggtagctta attagtgcta cgattgtatc
     4981 ggcatttatt accaacgtaa cgacatctga gcaatatatt tctattttat taccaggcag
     5041 aatgtatata aaagcctata gggataaggg actaagttcg agaaaccttt ctcgtgcatt
     5101 ggaagacgga ggaacagtta cttcggttct tgtaccttgg aatacctgtg gtgtttatgc
     5161 gttctcaatg ttgcaagtga gtgcttttga atatgcaccg tatgcaataa tgaattggct
     5221 cattccactg attgcgattg gcttggcaac cttgaatatc aaaattgagc gattgccacc
     5281 ggaagaatta gccaaagaac ctgaggaaat tactctgcca aaacgtggtt gttgtccata
     5341 atttatctaa atagtattta ataacaatag gttaagttaa ataacaagcg gtcggtttta
     5401 tcttaaaatt tgcaagttta tacataaatt tgatcgcttg ctgtatagaa aggaaaaatg
     5461 atgtcatacc aacttttacc taacaccact atccgtcctg aattgagccg tcattaccgc
     5521 tttgatgagc aaaccctggt gcaaaatctg atagaacaag cacattgtga tgaacaggct
     5581 gataacatta aaaagctcac tacccaatta gtcggtaaag tccgaactgc acgccagaaa
     5641 gcaagcgggg ttgatgcgtt aatgcacgaa ttttcactct ccagccaaga gggggtggcg
     5701 ttgatgtgcc ttgccgaagc attactacgc attcctgaca ccgccactgc ggataaatta
     5761 attcgagaca aaattgccaa aggggattgg cgttcacaca ccggcaacag cccctctctg
     5821 tttgtgaatg cagcggcgtg gggcttggtg attaccggca aattggtttc aacccacagc
     5881 gaaagctcac tctcatcagc actttcacgc ctgatcgcca aaggcggaga accgctgatc
     5941 cgcaaaggtg tggacgtagc aatgcgtatg ctcggcaaga aatttgtaac gggcgaaacc
     6001 attcaacagg cgattaaaaa cggcgaaaaa cgcttcgcaa tgggttatcg ttacagctac
     6061 gatatgctgg gcgaagcggc actcaccgcc gaagatgcca aacgctatta caacgactat
     6121 gttgcctcta ttcacgctgt gggtgccacc tcgaaaggct tgggcgtgta taaatcctcg
     6181 ggtgtgtcgg tcaaactctc cgccattcac cctcgttatt ccctttccca acaccaacgt
     6241 gtgatggacg aactctatcc acgtctgaaa gaactgttct tgctggcgaa aaagtatgac
     6301 atcggcttga acattgatgc cgaagaagcc gaccgcctcg aattgtcgct tgatttgatg
     6361 gacagactgc ttgccgaccc tgatttggca gggtttaacg gcattggttt tgtggtgcag
     6421 gcgtaccaaa aacgttgccc gtatgtgatc gagcatttaa ttgagaccgc ccgtaaacat
     6481 aatcgccagc taatgattcg cttggtaaaa ggcgcatatt gggacactga aatcaagcgg
     6541 gcgcaaatgg acggtgcaga gggctatccg gtcttctccc gtaaggtgca taccgatttg
     6601 aactacatcg tttgtgccaa aaaattgctg gcggcgcaag atgtgatcta tccgcaattt
     6661 gccacccaca acgctcaaac tctcgccacc atttaccact tggcacaggg caaacgtttt
     6721 gagtttcaat gcttgcacgg tatgggagag agcttgtacg accaagtggt tggcagcgat
     6781 aatcttaacg tgcaatgtcg catttatgcc cctgtcggta cttatcaaac cctgcttgcc
     6841 tatttagtac gtcgcttgtt ggaaaacgga gcgaacagct catttgttag ccaaattgtg
     6901 gatgaaaatc tcagtgtaga tgatctggct caagcccctg ttcaaaaagc agaactgacc
     6961 aatggcacta tgcacccgaa aattccattg ccagtcgatt tatttgacga caatcgtgtt
     7021 aattccaaag gctatgattt aactgatgca gtaagcctac aaaatttaga aaatgccctc
     7081 aatgaactta ccaaacagca ttaccgtgct gaaccacagc tggcaaatga taatacaagc
     7141 ggtcaagaaa gccgaaaaat ttacaatcca gcgaaccaag ctgaattagt cggcgaagtg
     7201 attgatgcca ccgaaaacga tatggaacag gcgtttgaaa gtgcggcggc gttccaaagt
     7261 gaatgggcga atacttcccc tgcggtgcgt gccgagtgct tagaaaaaat ggcggacttg
     7321 atggaagcca atatgccaca actgttcgat ttggcggtgc gtgaggcagg caaaacgctc
     7381 aacaatgcaa ttgctgaagt gcgtgaagcc gtcgatttct gtcgttatta tgccaaagag
     7441 gtacgcaaca acattgaagc atacaaaaat ccgcgtggca ttgtagtagc aattagcccg
     7501 tggaactttc cgttagcgat ttttgtgggt gaagtttcat ctgcgttggt ggcaggcaat
     7561 gtggtactag caaaaccagc tgaacaaact tcgttaatgg cacattatgc tgtcagcctt
     7621 ttctaccaag caggtgtgcc gaaagcggcg ttgcaatgct taccgggtag aggaaaaacg
     7681 gttggggtgg cgttggtggc agatcatcgt gtgaacggcg tgatttttac tggttcaaca
     7741 gagacagcta aaaacattaa ccgcaatttg cacaaaaacg agaaaattat accgcttatt
     7801 gccgaaactg gcggacaaaa tgcaatgatt gttgatagct cggcactggg cgagcaggtt
     7861 gtgatagatg ttttaaactc cgcatttgac tcagcagggc aacgttgttc ggcattgcgt
     7921 gtgctgtatc tgcaacgtga tgttgccgat cacattctca caatgctcaa aggagcaatg
     7981 gcggagttga aagttggtct tccacaacac ttaactaccg atgtcggacc agtaatcgat
     8041 aaagtcgcac aacaacgctt gctcgatcat attgaaaatc tcaaaaaatc ggcgaaaagt
     8101 tactatcagg tggctatctc ctctgaaatt gccgaaaatg gtattttcgt accgccaacg
     8161 ctgttagaaa tagatcatat cagccagctt aaacacgaag tattcggacc ggtgctacac
     8221 gttatccgtt ttgatgcgga taaactcgac caagtgattg ccgacataaa ctccacaggt
     8281 ttcggtctaa ccagcggttt gcatagccga attgatgaaa cggtggattt atggttgaaa
     8341 tctgtccacg ctggaaatct gtatgtaaac cgtaacacgg taggggctgt ggtcggcgta
     8401 cagccattcg gtggaatggg gttatcagga actgggccga aagctggcgg gccgctctat
     8461 ctacaacgtt tagtaaaccg ccaagaatgg acattgaacg gtttacaggg caatgcacaa
     8521 gcggtcgatt ttgacttaaa ttttgcaatc cgcaaggttc taagtggagc tgagcagcaa
     8581 acggcgttag atctggccaa aagccttgca cgccgctcgc cactcggaaa aacctaccca
     8641 atgcagggta ttaccggcga gaaaaacgag atgattttcg tggctcgccc acagattggg
     8701 attagcaaag gtaatttggc ttcacaggtg caagccttga ttgccattgt aacggtcggc
     8761 agccaagcgg tggcagaaaa atcaagcgag ctggcaaaac acgcctctgc ctttggtaag
     8821 aaattacagg tggtggatga tttcaacagt ctcgatcacc tcagcacact cattgtgctt
     8881 gacctgcttt ctagtgagga aaaattgcat catgccgaac gcaaaggggc gattttgacc
     8941 tacatcgaag ggctggattt agcgctctca ctgctgccgt tggtacacga gaaagcaatc
     9001 agcatcaaca ccgctgccgc agggggaaat gccagcttaa tgagtgaaat ggattaagca
     9061 acaaacaaaa gagtataaga gggcagatca caatgatctg cctttttatt ttaaggggct
     9121 gtcctagata acgactaaaa cttccattta ggttaagata accctatgag aaaaagtcgt
     9181 ctaagtcagt acaaacaaca taaactcata gagctgtttg tagcaggcgt aatcgctcga
     9241 acagccgcag agttagtgaa tgtaaataaa accaccgcag cctattattt tcaccgtttg
     9301 cgattgctca tctatcaaca tagccctcat ttggaaatgt ttgagggaga aattgagatt
     9361 gatgagagct atttcggcgg tcatcgcaag ggtaaacgag gtcgtggtgc agcaggcaaa
     9421 accgccgtat tcgggctttt aaagcgtgac ggcaaggttt ataccgtagt agtgccgaac
     9481 acacaatctg ctacactttt gccgattatt cgggaaaagg tgaagcccga tagccttgtt
     9541 tacaccgatt tttatcgaag ctatgatgtg cttgatgtga gtgaatttaa ccattttcgt
     9601 atcaatcaca gcacacactt cgcagaaaat caaaatcaca ttaacggaat tgagaatttt
     9661 tggagtcaag caaaacgtca tttacgcaag tttaatggta ttccgaaagc ccattttgag
     9721 ctatatttga aagaatgcga atggcgtttt aatcacagtg acttaaaatc tcaaatattc
     9781 cttttaaaac aattggttaa aggggggtta ggttagttat ctaggacagc ccctaaaagt
     9841 aatataaccg atatttaggc ctgacttttc aaataattga atatctttat tcgcttttaa
     9901 agcagacaaa tctgacgggt tcggataagg catcgcttgg cattcgttct tctccaattt
     9961 agctaaacgt actgaggcat cgggcgtaat actaaataca aggcgatcta tcttcgcttt
    10021 tccttcccaa tagtgttcaa atgctgtaaa tcttaccgct gcgtcctttt ggtaaccata
    10081 gaactggaat gcacctgtgc ctataggttc taaatctaat ttttccggcg tacccgctat
    10141 ttgcatttta tttgcatatt ccgcagaaaa gatcgccata aaatccatag ctaaattagc
    10201 aagaaagggg gcattcggtt cagataattc gatttttacc gtgtaatcat ctacttttgt
    10261 tacatttttg atgatgtttt gcatattcat tccgtagaaa aactcatagt taccgccgga
    10321 caatttgtga tatggattcg tcttatcaaa ctgacggtta atcgaaaaaa taacatcatc
    10381 agcattaaaa taacgactag gggtaaaact tttattactc tggaactgca cattctgacg
    10441 caagaaaaaa gtataaattt ttccgccttc actgatttcc catcgctctg ccagagaagg
    10501 agtgaggtta gtggtacctt tttcaaaatc aatcaggcga ttatagattg cttgcccgac
    10561 atcaagagta gctccatctg ttgcaatctg aggattcaat gctgccggag atgattccaa
    10621 acagtaagta aaagatttag gtgctgcgaa agcagaacct gtaaatgcta aagaaataag
    10681 tgcaagttgt gtcagcttgt tcattgtcat atccttaaat gttaagcaat aaaaaaccct
    10741 ttcaaaagtt gccttccgaa agggttagaa ttgggtttgc gattttggat tgcgtttgcc
    10801 atttcaggtt ttcggaagat atcttccgaa cacgccaaat gcccgaaaga ttaatcggtc
    10861 aaatggtagt gatgatgttt aacgcaatag ctcataatat tctccaaaat tgaatgttta
    10921 ggaatttacg ctttttaaaa atcaattgca aacgttttct ttaaaatttt gtaaaaaatt
    10981 caacattttt caccgcttgt atcgtaccta ggagcaaatg gcgatttgct cctaggtttc
    11041 caagcaatag gctatttttt cttcgcatat ttaagtgagt caatcgctac cgccaagata
    11101 atgatgccac ctttaataat atattgccag taagggttta ctccaatata ggttaagccg
    11161 tagttgataa cggtgaagat taacacccct gttactacac cgatgatagt acccacacca
    11221 ccggcaaagg ataccccacc taccacacaa gcggcaatgg catccatttc atacataaag
    11281 cctaagttgt tggttgcact accgatacga cctgcttcta acataccgcc gaaggcatag
    11341 aagatgcctg aaagcatata aatacccatc aggttaagcg gtacgtttac accggaaact
    11401 cgtgctgctt cagggttgcc gccaatagca aatacgtttt taccgaagcg ggttttattc
    11461 cataacgtcc acataataat ggtagcaatc acggcataaa tagtgatgta ggatagccta
    11521 aagtcgccaa agcggaagaa gccttgtgca aaggttgaga aatcatcgct aaagcctgca
    11581 attggcgatg agcctactgc atcgtagtaa agcgagttga taccatacac aataatcatt
    11641 gttcccatcg tggcaataaa cggtgttacg ttgaggtaag ccaccacaaa gccgttaaat
    11701 aaaccaatga cagcaccgat ggcacacacg gttaatacaa ccacaggaat cgggatttcc
    11761 gagaggtttg ggaatacctt gttcatattt tccattgatt ggagcaaggt ggcggaaatt
    11821 accgctgcca agcccacttg gcgaccggca gataagtctg tcccttgtgt tacaatcaag
    11881 cctgcaaccc ctaatgcaat aatcaaacgc acagaagatt gcgataaaat gttactgaag
    11941 ttacgcaagc tcaagaaact tgggtcttgc acaataatca caataagtaa gattaataat
    12001 acgaagtaaa tcgcattttg tttaaaaaaa tcgagtgttc tattttgttg taaagcagac
    12061 atacttcact ccttgttata aatattttgc tgcgagttgc aagatttctt cttgcgaggt
    12121 gtgagcggtt tcgacaatac ctgcaacttt cccgttactc atcactaaaa ttcggtctgt
    12181 gaccccaagc aattccggca tttccgatga aatcataatg atgcctttat ctttattcgc
    12241 cagctctaaa atcaactggt aaatttcaaa ttttgccccg atatcgatac cacgggtcgg
    12301 ctcgtcaagc atcaagattt caggctgggt taacaaccaa cgcccgataa tcactttttg
    12361 ttggttaccg ccggaaagtg agccaatatg cgtttgatgc gatggggttt ttacattcat
    12421 cgaatcaatt acccattggg tatcgctttt cattttttta ttgctcagta agccgaactt
    12481 gcctagataa gacttcatat ttgaaatcag agagttaaac tcaatgctta agttggcata
    12541 aatccctgtt gaacggcgtt cttctgtaac caatgcaaag ccgttattaa tcgcatcaaa
    12601 agcgttgcgg ttattgattt ctttatcgtt tagtcggata gtgccgcctg ctttctcccg
    12661 tacaccgaaa atggtttcaa caatatcagt ccgttttgct ccaactaagc cggcgatgcc
    12721 taaaatttcc cctctgcgta agctaaagct gacatcttga atagagggtt gattttttgc
    12781 cgttaaatgc tcaacttcaa gaatcacttc tttcggcaga ttggtttttt cagggaaacg
    12841 ctgggtcagc tcacgcccta ccatcattga aaccacttga tccattgagg tatctttaat
    12901 tgcaacggtg tttacccatt taccgtcacg caaaatggta atttcatcac aaattttaaa
    12961 aatttcgtcc attttgtgtg aaatatagat aatgccacag cctctgtctt tcagtttttg
    13021 gataatttta aataaatgct cgacttcttt ttctgaaagt gacgaagttg gttcgtccat
    13081 aatcacaatt ttggcattgt aagagaaagc ttttgcaatc tcgatcatct gcatttgtga
    13141 aaccgataat ttggctactt tatcacgtgg atcaacatca atatctaatt catcaaaaat
    13201 cgccttagta tcacgataca ttttatcgtg atctacaaaa ggccctttta gtgggtaacg
    13261 acctagccat aagttatcca ttaccgtcac ttgttttact aggttcaact cttggtgaac
    13321 cattgagatc ccattttcta acgcctcttt tgaggtctta aaattcaccg gcttgccaag
    13381 aaagagaata tctccctcat ctttagcata aatcccaaat aagcatttca gtaacgttga
    13441 tttacctgct ccattctctc ccattaaggc gtgaacacta tgggatttca ccgttaagtt
    13501 agcattatct aaggctttta ccccgggaaa tgacttgcta acatttgtca ttgtgagtaa
    13561 aacttcacct gtcatatctc acccctgatt acatcgaaaa atcgaaaaaa agcacgggta
    13621 tccatcaccc gtgctaaaag cgtgaattat tttaaaaatt cagataagtt gtctttatcg
    13681 acaccaacgt aaggaatacg caccacacgg ttttccaatt tccattgtgt accagcaact
    13741 gcatctttac cgtctgctaa gttagcagaa agctgaacaa ccgctttacc ttggtttaca
    13801 ccatcattta ataccgtacc ggctaattca ccttttttga ttaattgaag tgcttctggc
    13861 aatgcatcta caccgaagat tggtaatttt ctaccgtgag ctttggttgc ttctaatgca
    13921 cctaacgcca taccatcatt attagaaata attacttcga tgtctttcgc tttaccactt
    13981 gataaccaag catccacttt atctttagct tgggctgcat cccataatgc tgcgtccata
    14041 aagatttgct cactgtcgat accttgtgct tttaagcttt ctaccacata tttagtacgt
    14101 gcttccgcat cagggtgacc cggctcacct tttaataagg caaattggat tttaccgtct
    14161 ttattcaagt ctaattcagg atgggctttc cactgttttg caattaactc accttgaata
    14221 atgcctgact ctttcggctc agtacctaca taataagctt tcggatagct atcaatcgca
    14281 gctgcacccg ggtctttatt gaagaacacc acaggcacat cttcactttt tgctttttga
    14341 ataattgttt tataagctga tggatccact aagttaattg ctaatgcttt aaccccttta
    14401 gaaagtaata catcaacttg gtcgttttga atagattgcg tattttgcga gtcattcatt
    14461 aataactcga ctttttgtgt tttaccctct ttttcgattt ctttacgcat taatgccata
    14521 aagttatcgt catatttata aattgttaca ccaatacggt tttcagcttg agctgtactg
    14581 atcgttgcac ctaatgcgac tgccattgcc actgcactta atactgcttt cttcatagta
    14641 gatcacctct tttatgtgtt tatagggaaa tgaattgctt accataagca acttggtgca
    14701 aagtataact aattttaaaa aaacaatccg tgatcattct cacagaatta agacaaagtg
    14761 gagaaatttt taattttaag tataa
//
LOCUS       DANLUQ010000051        14003 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.051, whole genome shotgun sequence.
ACCESSION   DANLUQ010000051 DANLUQ010000000
VERSION     DANLUQ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 14003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14003
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.051"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(146..862)
                     /gene="rph"
                     /locus_tag="PXB27_001218"
     CDS             complement(146..862)
                     /gene="rph"
                     /locus_tag="PXB27_001218"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDL5346012.1"
                     /translation="MRPNNRANNQVREIKITRNYTRYAEGSVLIEFGETKVLCNATVE
                     ESVPRFLKGQQQGWVTAEYGMLPRATHSRTQREAAKGKQGGRTMEIQRLIARSLRAVV
                     DLKALGERTVTVDCDVIQADGGTRTASITGACVALYDAMNKLVANGTLKTNPMKGLVA
                     AISVGIVAGEAVCDLEYLEDSNAETDMNVVMVEDGRLVEVQGTAEGEPFSHQELLTLL
                     DLAKQGISQLVEAQRKALAE"
     gene            1143..2006
                     /locus_tag="PXB27_001219"
     CDS             1143..2006
                     /locus_tag="PXB27_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011272075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDL5346013.1"
                     /translation="MIYSMTAFAHLEMKKEWGNAVWEIRSVNQRFLETYFRLPEQFRN
                     LEMTLRERLRSALTRGKVECSLRIDLSKTQNSEIALNKEYAAQVVQSLKWLKETANEG
                     EINLVDVLRFPGVVDNESQDLDQIAQDLLAGFEQILADFIAMRAREGENVQALILQRL
                     EAISVEAAKVQAQMPEILQWQKDRLQQRFDELNLQLDPQRLEQEMVLLAQRVDVAEEL
                     DRLQLHVKETTNILKKGGAVGRKLDFMMQELNRESNTLASKSINADVTNSAVELKVLI
                     EQMREQIQNLE"
     gene            2015..2905
                     /locus_tag="PXB27_001220"
     CDS             2015..2905
                     /locus_tag="PXB27_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HDL5346014.1"
                     /translation="MAMNFKQVSQSYPNLCVPLSQYCLIEIAGVDAEKYLQGQLTCDV
                     AKISVGEHTLTSHCDPKGKMSALLRLYRAENEKFMAIIHRSLLPEALTQLKKYAVFSK
                     VTFTELDTPLYGMAGEAAFAKLSENMTALRLTTGQPRAIVWGEELETTADEQLWTLMD
                     IQDGIPVLLQQNQFELIPQATNLQALESAISFTKGCYIGQETVARAKYRGANKRALFT
                     LAGNFESEISLPEVASAIEMQLGENWRATGTVLTSATDENTLWVQVVLNKDVEPNTKF
                     RANGVALEMVELPYSLAENE"
     gene            complement(2937..3197)
                     /locus_tag="PXB27_001221"
     CDS             complement(2937..3197)
                     /locus_tag="PXB27_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HDL5346015.1"
                     /translation="MALLITHKCTNCDMCLPECPNEAISVGDDIYIIDPVLCTECVGH
                     YEKPTCQKVCPITNCIIKDPDNIETEEQLWERFVMIHHPDQI"
     gene            3427..3846
                     /gene="rbsD"
                     /locus_tag="PXB27_001222"
     CDS             3427..3846
                     /gene="rbsD"
                     /locus_tag="PXB27_001222"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005753460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDL5346016.1"
                     /translation="MKKTLNLNAQLSHSIATLGHTDSLTVCDAGLPIPNTIERIDLAL
                     TAGVPSFLQTFDVVVSELFVERVLLAEEIKHKNPQVLAALLERLTQLEQTQQNKIQID
                     YVNHEIFKEQTHYSKAVVRSGECSPYANIILYSGVPF"
     gene            3856..5352
                     /gene="rbsA"
                     /locus_tag="PXB27_001223"
     CDS             3856..5352
                     /gene="rbsA"
                     /locus_tag="PXB27_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HDL5346017.1"
                     /translation="METLLKMSGIDKEFPGVKALSNACLSVYGGRVMALMGENGAGKS
                     TLMKVLTGIYSKDAGSISYLGKEVTFKGPKDSQEAGISIIHQELNLVGNLTIAENIFL
                     GRESKTAWGAIDWKKMYAEADKLLARLGVKHSSHQLCAELSIGEQQMVEIAKALSFES
                     QVIIMDEPTDALTDTETASLFKVIRELKAENRGIVYISHRLKEIFEICDDVTVLRDGQ
                     FIGERVIADIDEDQLIEMMVGRRLEEQYPHLHQERGELLLEVKNLSGSGVNNVSFQLH
                     KGEIVGISGLMGAGRTELMKVLYGALPKTAGSIELNGKAISNHCPQDGLNNGIVYISE
                     DRKGDGLILGMSVKENMSLTSLDHFSKAGSIRHDAEKLAVDDFIEMFNIKTPSREQQI
                     GLLSGGNQQKVAIAKGLMTRPNVLILDEPTRGVDVGAKKEIYQLINEFKKDGLSILMV
                     SSDMPEVLGMSDRILVMHEGQISAEFSRAEATQEKLLAAAIGKSTGLN"
     gene            5362..6330
                     /gene="rbsC"
                     /locus_tag="PXB27_001224"
     CDS             5362..6330
                     /gene="rbsC"
                     /locus_tag="PXB27_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005753457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HDL5346018.1"
                     /translation="MATQTKQFQLGRFLIEQRSFVALFILIVSVSMINPDFFSVDNIL
                     NILRQTSVNAIIAVGMTFVILIAGIDLSVGSVLALTGAVAATLVGSELPILLVIPLVL
                     LLGTAFGGISGAIVAKGKVQAFIATLVTMTLLRGITMVYTDGRPISTGFSDQADAFAF
                     IGTGYLFGIPVPIWIMAVVFGLAWYILKHTPIGRYIYALGGNESATQLSGINVNKIKI
                     FVFAVSGFLSALAGLIVTSRLSSAQPTAGVSYELDAIAAVVVGGTSLMGGKGRVMGTL
                     VGALIIGFLNNALNLLDISSYYQMIAKALVILAAVLADNYLGTKKV"
     gene            6353..7231
                     /gene="rbsB"
                     /locus_tag="PXB27_001225"
     CDS             6353..7231
                     /gene="rbsB"
                     /locus_tag="PXB27_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006995182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HDL5346019.1"
                     /translation="MKKLTTIASAVMLSLSVAATASAKETIALAVSTLDNPFFVSLKD
                     GAQKKADELGYKLVVLDSQNDPAKELSNVEDLTVRGAKVLLINPTDSEAVGNAVAIAN
                     RNKIPVITLDRGAAKGEVVSHIASDNVAGGKMAGDFIAQKLGSGAKVIQLEGIAGTSA
                     ARERGEGFKQAIEAHKFNVLASQPADFDRTKGLNVTENLLSSKGDVQAIFAQNDEMAL
                     GALRAVSSAKKEVVIVGFDGTDDGVKAVESGKLAATIAQQPELIGSLGVETADKVLKG
                     EKVEAKIPVDLKVISK"
     gene            7352..8281
                     /gene="rbsK"
                     /locus_tag="PXB27_001226"
     CDS             7352..8281
                     /gene="rbsK"
                     /locus_tag="PXB27_001226"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDL5346020.1"
                     /translation="MKKLTVLGSINADHVISVPYFAKPGETLTGHSCHIAYGGKGANQ
                     AVAAARLGAKVSFIGCIGSDDIGKAMKSAFEQDGIDTRSIKTIPNEMTGIAMIQVAES
                     GENSIVISAGANGHLDETVVAKCAAEITQADYLLMQLETPLPAIVQAAKLAQQNGTKV
                     ILNPAPARALPDELLNLLDMITPNETEAEILTGVKVVDEASAIQAAQVFHQKGIEKVL
                     ITLGSKGVFVSENGVGKIVAGFRVQAVDTTAAGDTFNGALVTAMLEDKPLDQAIRFAH
                     AAAAISVTRKGAQPSIPTRQETLDFLSQQAVSF"
     gene            8295..9305
                     /locus_tag="PXB27_001227"
     CDS             8295..9305
                     /locus_tag="PXB27_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL5346021.1"
                     /translation="MATMKDIARLAQVSTSTVSHVINNSRFVSEEIRERVQRVVKELN
                     YTPSALARSLKVNETKTIGMLVTATGNPFFAEVMAGVEQYCQQHQYNLIIATTNGDTE
                     RLQHHLQMLMQRKVDGLLLMCGDTRLNADESLNIPLPLVVLDWWFTELNADKIFENSI
                     SGGYLATKTLIEAGHSKIAIITGNLKKSLAKNRLEGYKKALAEANIPLNPNWVFESHF
                     DFAGGVNGMNALLAQSEKPTAVFACSDTIALGAYQAIWQQGLRIPQDISIIGYDDIEL
                     AKYLSPPLSTISQPKAELGRLAVETLLKRIRFKSTDFQTIMLEPELVLRHSIQTINKN
                     AE"
     gene            complement(9341..9970)
                     /locus_tag="PXB27_001228"
     CDS             complement(9341..9970)
                     /locus_tag="PXB27_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL5346022.1"
                     /translation="MTAPTQILLIDDHPLMRQGMRQLLELDHQFSVVGEASNGTDGIK
                     LALRLEPDVIILDLNMKGLSGLDTLRSLRAQGIDARIIVLTVSDEQSDVFALMDAGVD
                     GYLLKDTDTPELLDNIRKAAAGEVVLSETVRQHLLNRQPENGPLSCLTDRELDVLQWL
                     ATGMSNKQIAAQLFISEETVKVHIRNLLRKLNVHSRVAATVLYLEQQKG"
     gene            10168..12327
                     /gene="rlmKL"
                     /locus_tag="PXB27_001229"
     CDS             10168..12327
                     /gene="rlmKL"
                     /locus_tag="PXB27_001229"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HDL5346023.1"
                     /translation="MTNQTTYFATAARGFEEILKTELEQICAAECKVAQGGVHFTTTQ
                     KGAYQALLHSRLASRILLPLISTKIFSDSDLYASIVSFNWADLFDPRDTFYIDFSGTN
                     REIRNTQFGAMRVKDGIVDYFERKGFARPTVDKDNPDVRIHVYLDRENVVVSLDLSGD
                     ALHIRGYREETGKAPLRETLAAAIILRSGWKVGTPLVDPMCGSGTLLIEAAQMAANIA
                     PQLHRKHWGFNAWKGHQQAMWKAVLDEAFRNVELGAVGENCNSSLQKMFFGFDLDHRV
                     LTKAKQNAKNAGVDHLIQWQQGDVAALKNPIPEKIGTVVCNPPYGERLGTTPALIALY
                     SVFGQRLKQQFAGWNASIFSGEPELLNCLRLRSARQFKAKNGPLDCVQKNYSISEHNK
                     RSDLDENLQFEQNAQVAPDFANRLTKNIKKIEKWAKQQGIDAYRLYDADLPEYNLAVD
                     RYGDHIVVQEYQAPKNIDEQKARQRLLDAVSATLYVTGVETNKLVLKVRQKQKGTNQY
                     EKLANKGEYFYVNEYGAKLWVNLTDYLDTGLFLDHRLTRKMVGEMAKGKTFLNLFAYT
                     GSATVHAALNGAKSTTTVDMSNTYLNWAEQNLELNNLSLRHHRLFQADCLQWLAECRE
                     RFELIFVDPPTFSNSKRMENSWDVQRDHIELMKQLKRILTADGTIVFSNNKRGFKMDF
                     DGLAALGLTAENISHKTLPLDFERNPQIHNCWIVKNV"
     gene            12504..12983
                     /gene="trmL"
                     /locus_tag="PXB27_001230"
     CDS             12504..12983
                     /gene="trmL"
                     /locus_tag="PXB27_001230"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDL5346024.1"
                     /translation="MLDIVLYEPEIPQNSGNIIRLCANCGFRLHMIEPFGFGWDDKKL
                     RRSGLDYHEFVDIQKYKNFEDFLERAKPKRLFALTTRGEQNHSDVKYELGDFLMFGPE
                     SRGIPKPLLAEMPMSQKIRIPMCKDSRSMNLSNSVAVVVYEAWRQFGYQNAVAAQSI"
     gene            complement(13077..13895)
                     /locus_tag="PXB27_001231"
     CDS             complement(13077..13895)
                     /locus_tag="PXB27_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HDL5346025.1"
                     /translation="MQEIKLFEQKSVRSHYDEEKDKWYFSIIDVIAILTESLDPSAYW
                     RKLKQRLKAEGNETVTNCHGLKMPSADGKMRLTDVADTEQLFRLIQSIPSPKAEPFKQ
                     WLAKVGYERLQEINDPSQSLDRARENWQKMGRSEKWIQQRMMGQETRNKLTDYWKSSG
                     VEQPQDFAFLTNVIHQEWSGLSVQKHKSLKGLKSQNLRDHMSEAELLFTALAELSSRQ
                     IAESMQAVGVDENAQAGKQGGNIAKNARQELENKTGKSVVTGENFLPPAKNEEE"
BASE COUNT         4061 a         2731 c         3236 g         3975 t
ORIGIN      
        1 tatttccata aaaaaataat tgttatttta tgattgagtg tattttgaaa agggaaataa
       61 aaaaatctac atctgaatca gaacggttat tctgttttgt cagatgtaga ttttatttaa
      121 aaagaaaatc aaaaagagaa gtgaattatt cagctaacgc tttacgttga gcttcaacta
      181 gctggctaat gccttgcttc gctaaatcta gcaatgtcag taattcttgg tgcgagaacg
      241 gttcgccttc tgccgtgcct tgtacttcca ctaaacgacc atcttcgacc atcaccacgt
      301 tcatatcagt ttcagcgttg gaatcttcca gatattctaa atcgcacacc gcttcgcctg
      361 ctacaatacc gacagaaatt gctgcgacta aacctttcat tgggttagtt tttagtgttc
      421 cattagctac taacttattc attgcatcgt acaatgctac gcacgcaccg gtaattgagg
      481 cggtgcgagt gcctccatcg gcttgaataa catcacaatc tacggtaacg gtacgttcgc
      541 ctaaggcttt taaatctacc accgcacgca gagaacgggc aatcaagcgt tggatttcca
      601 ttgtgcgtcc gccttgtttg ccttttgccg cttcacgctg agtgcgagag tgggtggctc
      661 gtggcaacat accatattct gcggtaaccc aaccttgttg ctgacctttt aaaaagcgtg
      721 gcacgctttc ttctaccgta gcgttacaca atactttggt ttcgccaaac tcaattaata
      781 ccgagccttc ggcatagcga gtgtagttac gggtaatttt aatttctcta acttggttgt
      841 ttgctcgatt atttggacgc attttctttt ccttttgcta aaatttttac cattttagca
      901 aagaacacag taaaatgtga tttttcttta gcaaagaatt tacatagccc tcgccccttg
      961 tgggagaggg acggattttt cttcgttcag aagaaaaatc agggagaggg gtaagcggtt
     1021 atttttatct caaattttac aaattcctcc ctctctctga aaaatatttc tgaacgaaat
     1081 atttttcggt ctctctccct caaggggaga gagctttact tataaaaaca caaggaatac
     1141 caatgattta tagtatgaca gcatttgccc atttagaaat gaaaaaagag tggggcaatg
     1201 cggtgtggga aattcgctcg gtaaatcaac gctttttaga aacctatttt cgtttgccgg
     1261 agcagtttcg caacctagaa atgaccttgc gtgagcgttt acgttctgca ctgacacgag
     1321 gcaaagtaga gtgtagctta cgcattgatt taagcaaaac ccaaaacagt gaaattgcgt
     1381 taaacaaaga atatgccgca caggtggttc aatcattaaa atggttaaaa gaaaccgcaa
     1441 atgaaggcga aatcaacctt gttgatgtgc tacgtttccc gggcgtggtg gataatgaaa
     1501 gccaagattt agaccaaatt gctcaagatt tattagccgg ctttgaacaa attctggcag
     1561 attttatcgc aatgcgagcc agagagggcg agaacgtgca agccctgatc ttacaacgtt
     1621 tagaagcaat ttcagttgag gctgcaaagg tacaagccca aatgccggaa attctgcaat
     1681 ggcagaaaga tcgtttacaa caacggttcg atgaactgaa tttacagctt gatccacaac
     1741 gcttagaaca agaaatggtg ttacttgccc aacgtgttga tgtggcggag gaattagacc
     1801 gcttgcaatt acacgttaaa gaaaccacca atatcctgaa aaaaggcggt gcggttgggc
     1861 gtaaactgga ttttatgatg caagaactaa accgtgaatc caacacgctc gcctcaaaat
     1921 cgattaatgc ggacgtgacc aattcggcgg tggagctgaa agtgttgatc gaacagatgc
     1981 gtgaacaaat tcagaattta gaataggaca gattatggca atgaatttca agcaagtgag
     2041 ccagagctat ccaaatctct gcgtgccgct ttctcagtat tgtttaattg aaatcgcagg
     2101 ggtagatgct gagaaatatc ttcaagggca attaacctgc gatgtggcaa aaatttcggt
     2161 gggcgaacat acgctaacca gccattgtga tccgaaaggc aaaatgagtg cgttactgcg
     2221 tttgtatcgt gcagaaaatg agaaatttat ggcgatcatt caccgcagct tattgcctga
     2281 agccctgacg caacttaaaa aatatgcggt tttttcaaaa gtgaccttca cggaattaga
     2341 caccccactt tatggtatgg caggtgaagc agcatttgca aaattaagcg aaaatatgac
     2401 cgctttgcgt ttaaccacag gacagcctcg ggccattgtt tggggcgaag agcttgaaac
     2461 gactgccgat gaacagctct ggacattaat ggatattcaa gacggcattc ctgttctctt
     2521 gcaacaaaac cagtttgagc tgattccaca ggcaaccaat ttacaggcac ttgaatctgc
     2581 gatttcattc accaaaggtt gctacatcgg gcaagaaacg gtagcgagag cgaaatatcg
     2641 tggggcgaat aaacgtgctt tatttaccct tgccggcaac tttgaaagcg aaatcagctt
     2701 accggaagtt gcttctgcga ttgaaatgca actgggcgaa aattggcgag caaccggcac
     2761 tgtgttgacc agtgcgactg atgaaaatac cctgtgggtg caagtggtgc tgaataaaga
     2821 tgttgaacct aacaccaagt ttagagccaa tggtgtagcg ttggaaatgg tcgagttacc
     2881 ttatagtttg gcagaaaatg agtaaaacga acggtcatca gggtatgacc gtttttttat
     2941 atctgatcag ggtgatgaat catcacaaaa cgttcccaca actgctcttc ggtttcgata
     3001 ttatcaggat ctttgataat gcagttggta atcgggcaaa ctttctggca agtcggcttt
     3061 tcataatgcc cgacacattc ggtacataac accggatcga tgatataaat atcatcgccc
     3121 accgaaatcg cctcgttcgg gcattccggc aagcacatat cacagttggt acatttgtgg
     3181 gtaattaata aagccatagt ttatattgag aaaataaaaa ttaaacaagc ggtcgatttt
     3241 gcaaaaaaat ttgcaaatct taccgcttgt cactaaatgc tacctattat aaagcaaaat
     3301 aatttgaact acttcacatt tttgcaattt ggttgtttat ccgattaaga aaatttttta
     3361 tgatgccatc gaaacgtttc gatggagtat tttccaacgt gatggagcga aaataaaggg
     3421 gttagtatga aaaaaacgct aaatctaaat gcacaacttt cgcacagtat tgcgacactc
     3481 gggcataccg atagtttaac ggtttgcgat gccggtttgc cgattccaaa cacaatagag
     3541 cggatcgatt tggctttaac cgccggtgtg ccaagttttt tacaaacatt tgatgtagta
     3601 gttagtgaat tatttgttga acgagtgcta cttgctgaag aaattaaaca taaaaatccg
     3661 caagtgttag cagctctttt ggagcgttta acccaattag aacaaactca acagaataaa
     3721 attcaaattg attatgtgaa ccacgaaatc tttaaagagc agacacatta cagtaaagct
     3781 gttgtgcgtt cgggtgagtg ttcaccgtat gccaatatta ttttatattc gggcgtgccg
     3841 ttttagggtg gaatgatgga aaccttatta aaaatgagcg ggatagataa agaatttccc
     3901 ggcgtgaaag ccttgagcaa tgcgtgctta tctgtttacg gtgggcgagt aatggcatta
     3961 atgggcgaaa atggggcagg taaatccacc ttgatgaaag tattgacggg gatttatagc
     4021 aaagatgccg gctctattag ctatttaggc aaagaagtta cctttaaagg cccgaaagat
     4081 tcacaagaag ccggtattag cattattcac caagagctga atttagtcgg taatttaacc
     4141 attgctgaaa atattttttt gggccgtgag tccaaaaccg cttggggagc tattgattgg
     4201 aaaaaaatgt atgccgaggc ggataaactg cttgcccgac ttggggtaaa acacagtagc
     4261 catcagcttt gtgcggagtt atccattggc gagcagcaaa tggtggaaat tgcgaaagcc
     4321 ctgagttttg agtctcaagt aattattatg gacgaaccga ccgatgcttt aaccgataca
     4381 gaaacagcat cgttgtttaa agttattcga gagttaaaag cggaaaatcg aggcattgtt
     4441 tatatttccc atcgtttaaa agagattttt gaaatttgtg atgatgtgac ggtgctaaga
     4501 gacgggcaat ttatcggcga gcgagtaatt gccgatattg atgaagatca gttaattgaa
     4561 atgatggttg gtcgccgttt ggaagagcaa tatccgcatt tacaccaaga gcgtggcgag
     4621 ttattacttg aagtgaagaa tttaagtgga agtggggtga ataatgtgtc attccaatta
     4681 cacaaaggtg aaattgtggg tatttccggc ttaatggggg cagggcgtac agaattaatg
     4741 aaggtgcttt atggtgcatt gccgaaaaca gccggctcga tagagttaaa tggaaaagcg
     4801 atttccaacc attgtccgca agatgggttg aataacggca ttgtgtatat ttcagaagat
     4861 cgtaaaggcg atggtctgat tttgggaatg tcggtaaaag aaaatatgtc tttgacctct
     4921 ctcgatcatt tctccaaagc cggcagtatt cgccacgatg cagaaaaatt ggcggtcgat
     4981 gattttattg agatgtttaa cattaaaacc ccaagtcggg agcaacaaat cgggctgctt
     5041 tccggtggta atcagcaaaa agtggcgatc gctaaagggt tgatgacccg cccgaatgtg
     5101 ctgattttag acgagccgac acggggtgtt gatgtggggg cgaaaaaaga gatctatcaa
     5161 ttaattaatg aatttaaaaa agatgggctt agtattttga tggtttcgtc cgatatgccg
     5221 gaagtgttgg gaatgagcga ccgcatttta gtgatgcacg aagggcaaat cagtgcagaa
     5281 ttttcacgag cagaagcgac acaagaaaag ctactggcag cagctattgg caaaagtaca
     5341 ggattaaatt aaggtgtgtt tatggcaact caaacaaagc agtttcaact tggcagattt
     5401 ttaatcgagc aacgttcttt tgtagcgtta tttattttaa ttgtcagtgt ttcaatgatt
     5461 aaccctgatt ttttcagcgt ggataatatt ttaaatattc tacgccaaac ctcggtgaat
     5521 gcgattattg cggtcggaat gacctttgtg attttaattg caggcattga tttatcggtc
     5581 ggttcggttt tagccttaac cggtgcggtt gcagcgacct tagtcggttc tgaattgccg
     5641 attttgttgg tgattccgct ggttttatta ttagggacgg catttggtgg tattagtggg
     5701 gcgattgttg ctaaaggtaa agtgcaggca tttattgcga ctttggtcac aatgacctta
     5761 ttgcgtggga ttacgatggt ttataccgat ggcagaccga ttagcaccgg cttttctgac
     5821 caagcagacg cctttgcgtt tattgggaca ggctatttat ttggtattcc tgtgccgatt
     5881 tggattatgg cggtagtgtt tgggttagct tggtatattt tgaaacacac cccgattggg
     5941 cgttacattt acgctttagg tggcaacgaa tcggcaaccc agctttcagg catcaatgtg
     6001 aataaaatta agatcttcgt atttgccgtg agtggttttt tatctgctct tgccggctta
     6061 attgtgactt cacgtctctc ttctgctcag ccaacggcag gtgtgtccta tgagttagac
     6121 gcaattgcag cggtagttgt tggtggaacc agtctaatgg gcggtaaagg gcgtgtaatg
     6181 ggaacgcttg tcggggcatt aattatcggt ttcttaaaca atgcccttaa tttattggat
     6241 atttcttcat attatcagat gattgctaaa gcgttggtta ttttagcagc tgtattagct
     6301 gataattatc taggcactaa aaaagtttaa tctatcttta aaggagattt ctatgaaaaa
     6361 attaactacg attgcatcag cggttatgtt aagtttatcg gttgcagcca ctgcttcggc
     6421 aaaagagact atcgctcttg cagtatcgac tttagataac ccattctttg tgagccttaa
     6481 agacggtgca cagaaaaaag cagatgaatt aggctacaaa ttagtggtgc ttgattccca
     6541 aaatgacccg gctaaagagc tttccaatgt ggaagactta acggtgcgtg gggcaaaagt
     6601 gttattgatt aacccaaccg attcggaagc ggtcggaaat gcggtggcta ttgcaaatcg
     6661 caacaaaatt ccggttatta cacttgaccg tggtgcagca aaaggcgaag tggttagcca
     6721 tatcgcttcc gataacgttg ccggcggcaa aatggcggga gatttcattg ctcagaaatt
     6781 aggctcaggg gcgaaagtga ttcaattaga aggcattgcc ggcacttccg ccgctcgtga
     6841 acgtggtgaa ggctttaaac aagcgattga agcacataaa ttcaatgtat tagccagtca
     6901 accggctgat tttgaccgca ctaaaggctt aaatgtgact gaaaacttgc tctcaagcaa
     6961 aggggacgta caagctattt ttgctcaaaa tgatgaaatg gcactaggag ctttgcgtgc
     7021 agtgagttca gcgaagaaag aggttgtaat tgtgggcttt gacggcacag atgatggcgt
     7081 aaaagccgtt gaaagcggta aacttgccgc aacgattgct caacaacctg aactgattgg
     7141 ctcattaggc gtagaaactg ccgataaagt gctaaaaggc gaaaaagtgg aagcaaaaat
     7201 tccggttgat ttaaaggtta tttcaaaata attatacttg aagatagcgt tcgtctctga
     7261 cgaacgcttt gaattaataa aatagcactc gtcagagact agtgctatca gaatcctagt
     7321 tttgttgtac taggtaattt gagggcttaa tatgaaaaaa ctcaccgttc tcggtagcat
     7381 taacgctgat cacgtgattt ccgttcccta ttttgccaaa ccgggggaaa cgctcaccgg
     7441 acatagctgc cacattgctt atggcggtaa aggggcaaat caggcggtgg cggcagcacg
     7501 tttgggagct aaggtttctt tcatcggttg tattggcagt gatgacatcg gcaaagcaat
     7561 gaaatcagcc tttgagcaag atggcattga cactcgctcg attaaaacga ttccgaatga
     7621 aatgaccggc attgcaatga ttcaagtggc ggaatcgggc gagaacagta ttgtgatttc
     7681 agccggagcg aacggtcatt tggatgaaac ggttgtggca aagtgtgcag ccgaaatcac
     7741 acaggcggat tatttgctaa tgcagctaga aacaccgttg ccggcgattg tgcaagcagc
     7801 aaaattggct cagcaaaacg gcacgaaagt aatattaaac cctgcgcctg ctcgagcgtt
     7861 acccgatgaa ctgttaaact tactggatat gattacccca aatgaaacgg aagcggaaat
     7921 attaaccggt gtgaaagttg ttgatgaagc cagtgcgatt caagccgcac aagtgtttca
     7981 ccaaaagggc atcgaaaaag tgttgattac acttggctca aaaggggtat ttgtaagcga
     8041 aaacggtgta ggcaaaatcg ttgcaggctt ccgtgttcaa gcggtggata ccactgccgc
     8101 aggggatact tttaacggag cattagtgac tgcaatgtta gaagataaac cgctggatca
     8161 agccattcgt tttgctcacg cagcggcagc gattagtgtg actcgcaaag gggctcaacc
     8221 ttctattccg acccgtcagg aaacactgga ttttctaagc caacaagcgg tgagttttta
     8281 ggaaaatttt gcaaatggca acaatgaagg atattgcacg gcttgcacag gtttccacct
     8341 cgaccgtttc tcacgttatc aacaatagcc gatttgtcag cgaggaaatt cgggaaagag
     8401 tgcagagggt ggtgaaagaa ctgaattaca cgccttctgc attggctcgt agcctcaaag
     8461 tgaatgaaac taaaaccatc ggaatgctgg taacggcaac cggcaatccg ttttttgcgg
     8521 aggtgatggc gggggtggag caatattgcc agcaacatca atataacctg attattgcca
     8581 ctaccaacgg tgacaccgag cgtttgcaac accatttgca aatgctgatg caacgaaaag
     8641 tagatggctt gttactgatg tgtggcgata ccagattgaa tgccgatgag agcttgaata
     8701 ttccgctgcc attggtggtg ttagactggt ggtttaccga gctcaatgca gataaaatct
     8761 ttgaaaattc aatatccggc ggctatctcg caacaaaaac cttaattgaa gcagggcatt
     8821 ctaagattgc gattattact ggaaacctaa aaaaatcatt ggcaaaaaat cgtttagaag
     8881 gctacaaaaa agcacttgcc gaggcgaata ttccactcaa tccaaactgg gtgttcgaaa
     8941 gccattttga ttttgccggc ggggttaatg gaatgaatgc tttgcttgcc caaagcgaaa
     9001 agccgacagc ggtatttgcc tgtagcgaca ccattgctct aggggcttac caagcaatct
     9061 ggcaacaagg tttacgaata ccgcaagata tttcgattat cggctatgac gatattgaac
     9121 tggcaaaata cctttcccca ccgctgagta ccattagcca gcccaaagcg gaactcgggc
     9181 ggcttgcggt ggaaactttg ctaaaacgaa ttcgttttaa atccactgat tttcaaacca
     9241 taatgcttga acctgaatta gttttaaggc attcaatcca aacaataaac aaaaacgctg
     9301 aataatgtga agttattcag cgttttttat ccgatttcaa ttagcctttt tgctgttcca
     9361 aataaagcac ggtggcggca actcgggaat gcacgtttaa tttacgcagt aaattgcgga
     9421 tatgcacttt tacggtttct tcggaaataa agagctgagc cgcaatttgt ttgttcgaca
     9481 taccggttgc gagccattgt aaaacatcca gttcacgatc tgttaagcaa cttaaaggac
     9541 cattttctgg ctggcggttg agtaaatgtt ggcgaacggt ttcgcttaat accacttcac
     9601 cggcagccgc tttacggata ttatctagta gttccggggt gtcggtatct tttaataaat
     9661 agccgtccac accagcatcc attaatgcaa atacatcgga ttgctcgtct gacactgtta
     9721 aaacaatgat tctggcatct ataccttgag cgcgcaatga gcgtaaagta tctagccctg
     9781 aaagcccttt catatttaaa tccagaataa tcacatccgg ctcaagtcgt aatgcaagtt
     9841 tgataccgtc ggtgccgttg cttgcttcac ccactacact aaattggtga tctaattcca
     9901 atagttgacg cattccctga cgcattaatg ggtggtcgtc aatgagtaaa atttgagttg
     9961 gagctgtcat ttatttctcc tttcctttct cttaatcggt taattttgca attattaatt
    10021 caaaatagca agtttaggga tttactaaat gccgcttttt tgatcttagc taatttttaa
    10081 ctaaaaaaga aattgcaaaa ctttgcaaga aaaagaccgc ttgttcggct ataattactg
    10141 ccaattttta tttcaagtgt gaaaagaatg acaaatcaaa caacctattt tgccacggca
    10201 gcccgtggtt ttgaggaaat actaaaaacc gaactggagc agatttgtgc ggcagaatgc
    10261 aaagtcgctc aaggcggtgt gcattttacc accacgcaaa aaggggctta tcaggcatta
    10321 ttacacagcc gtttagcctc tcggatctta ctgccgctca tcagcaccaa aatttttagt
    10381 gatagcgatt tatatgcttc catcgtgtca tttaactggg cggatttatt cgatccccgt
    10441 gatacctttt acattgattt cagcggcact aaccgagaaa tccgcaacac ccaatttggg
    10501 gcaatgcgag tgaaagacgg cattgtggat tactttgaac gcaaaggttt tgcccgccca
    10561 acagtagata aagataatcc ggatgttcgc attcatgttt atttggatcg tgaaaatgtt
    10621 gtggtttcgc ttgatttaag tggagatgcc ttacatattc ggggctatcg ggaagaaacc
    10681 ggtaaagctc cgttgcgtga aacattggcg gcagccatta ttttgcgttc cggctggaaa
    10741 gtcggcacgc cattggttga tccgatgtgt ggttcaggca cgttactgat tgaagcagca
    10801 caaatggcag cgaatattgc ccctcaactt caccgtaaac attggggctt taatgcgtgg
    10861 aaagggcatc aacaagcgat gtggaaagcc gtgttagatg aggcattccg aaatgttgaa
    10921 ttgggtgcgg taggggagaa ttgcaattcg tccctacaaa aaatgttctt cggttttgat
    10981 ctcgatcatc gtgtgctgac taaagcaaag caaaatgcga aaaatgcagg ggtagatcat
    11041 ctcattcaat ggcaacaagg tgatgtggct gcgttaaaaa atccgattcc ggaaaaaatc
    11101 ggcacggtgg tgtgtaaccc cccttatggt gaacgtttag gcacaacgcc ggcgttaatt
    11161 gctctttatt cggtgttcgg gcaacgccta aaacagcaat ttgccggttg gaatgcctca
    11221 atttttagcg gcgagcctga attgttaaat tgcctgcgtt tacgttcagc ccgtcaattc
    11281 aaagcgaaaa acggtccgtt ggattgtgtg cagaaaaatt actccatcag cgagcataac
    11341 aagcggtcag atttagatga aaatttacaa tttgaacaga atgctcaagt cgctccggat
    11401 tttgcaaatc gcttaaccaa aaatatcaaa aagatcgaaa aatgggcgaa acaacaaggc
    11461 attgatgcct accgtttata cgatgccgat ctgcctgaat ataatttagc ggtggacaga
    11521 tatggcgacc acattgttgt gcaggaatac caagccccga aaaatattga tgagcaaaaa
    11581 gcccgccaac gcttgttaga tgcagtctct gctacgcttt atgtgacagg tgttgaaact
    11641 aataaattag tgttgaaagt tcgccaaaaa caaaaaggca cgaatcagta cgaaaaactt
    11701 gccaataaag gcgagtattt ctatgtgaac gagtatgggg cgaaactgtg ggtgaattta
    11761 accgattact tggatacggg tttgttccta gaccatcgct taacccgtaa aatggtgggc
    11821 gaaatggcaa aaggcaaaac attcctgaat ctgtttgcct acacgggttc agctacggtt
    11881 cacgctgctc ttaatggggc aaaatcgacc actacagtgg atatgtcgaa cacctatttg
    11941 aactgggcgg agcaaaattt agagctgaat aatttatcac ttcgccatca tcgtttgttc
    12001 caagccgatt gcttgcaatg gctggcagaa tgccgtgagc gatttgagct gatttttgtc
    12061 gatccgccga ctttctccaa ctcaaaacga atggaaaaca gttgggacgt gcaacgagac
    12121 catattgagt taatgaaaca gcttaaacgt attttaaccg ctgatggtac cattgtgttc
    12181 tcgaacaata agcgtggctt taaaatggat tttgacggtt tggcagcatt aggcttaacg
    12241 gcggaaaata tttcgcataa aaccttaccg ttggattttg aacgcaatcc gcaaatccat
    12301 aattgttgga ttgtgaagaa tgtatagata ataccctcga cccttgtggg agagggatag
    12361 atttttcttc gttcagaaga aaaatcaggg agagggtaag aagttactga tgaaagagtt
    12421 atttccccct ctctctgaaa aatatttctg aacgaaatat ttttcggtct ctctcccaca
    12481 aggggagaga gtaaggctaa aaaatgttag atattgtttt atacgaacct gaaattccgc
    12541 aaaacagcgg aaatattatc cgcctttgtg ccaactgtgg ctttcgtttg catatgattg
    12601 aaccgttcgg ctttggttgg gacgataaaa agctccgccg ttcggggctg gattatcacg
    12661 aatttgtgga tattcaaaaa tataaaaatt ttgaagattt ccttgagcgt gctaagccta
    12721 aacgcttgtt cgccttaacc accagagggg agcagaacca cagtgatgtg aaatatgaac
    12781 ttggcgattt tttaatgttc gggccggaaa gcagaggcat tccaaagcca ttattagcgg
    12841 aaatgccgat gtcgcaaaaa atccgcattc cgatgtgcaa agacagccgc agtatgaatt
    12901 tatctaactc ggtggcggtg gttgtgtatg aggcgtggcg acaattcggc taccaaaatg
    12961 cggtagcggc acaatctatt taacttgcaa gcggtgtgat tttggctatt ttttgcaagt
    13021 ttgtggtcaa ggttggggga ggctcgacct tgactaatta agaacatcta ttgtgtctat
    13081 tcttcttcat ttttagctgg aggtaaaaaa ttctctccgg taacaactga tttacctgtt
    13141 ttattctcca attcctgacg ggcatttttg gcaatattgc caccttgttt tcctgcttgg
    13201 gcattttcat caaccccaac tgcctgcata ctttctgcaa tttgccgact ggaaagttca
    13261 gcaagtgctg taaataacaa ttcagcttcg ctcatatgat ctcgcagatt ttggcttttt
    13321 aaacctttaa gcgatttatg cttttgtaca gatagtccgc tccattcttg atgaatcaca
    13381 ttggttaaaa atgcaaaatc ttgtggctgt tcaacccctg acgatttcca ataatcggtc
    13441 agtttatttc gggtttcttg tcccatcatt ctttgctgaa tccatttttc acttcgtccc
    13501 atcttctgcc aattctccct tgctcgatct agactttgac ttggatcgtt tatttcctgt
    13561 aagcgttcat aacctacttt tgctaaccat tgtttgaagg gctctgcttt cggactaggg
    13621 atagattgaa taagtcggaa aagttgttcg gtatctgcta catcagtcaa acgcattttt
    13681 ccgtcagcag agggcatttt caacccgtga caatttgtca cggtttcatt gccttctgcc
    13741 tttagcctct gttttagctt acgccaataa gcagagggat ctaggctttc ggttaaaata
    13801 gcaatcacat caataataga aaaataccat ttatcttttt cttcatcgta atgactgcgt
    13861 acagatttct gttcaaaaag tttaatttct tgcataataa aaatactgta tataaaaaca
    13921 agaaaagtat aacagttgtc agcttttcag caagtagcaa aagtgatttt ttcgatctcg
    13981 atcccaaaaa tgatttttgg gta
//
LOCUS       DANLUQ010000052        13926 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.052, whole genome shotgun sequence.
ACCESSION   DANLUQ010000052 DANLUQ010000000
VERSION     DANLUQ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13926
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.052"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(82..2271)
                     /gene="yccS"
                     /locus_tag="PXB27_001232"
     CDS             complement(82..2271)
                     /gene="yccS"
                     /locus_tag="PXB27_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HDL5346026.1"
                     /translation="MNKFLRYLQHSWLSENVIKTLPMFISLNIAAVSIWQLGISDFAM
                     PLMLGIIAGGLVDLDNSLIGRAKNLIISLVAFSLSSIGASVSLHLGWLFVPVAVVCTF
                     LLVMLGAIGQRYSTIAFGTLIVAVYNSLAYTPEVLWYHNVVMILLGTLLYGLVGILVY
                     LIFPNRTVQENLASAYLALSHYLLAKSAYFDPDDDDLAAKQLALAKANSEVMAAFDKT
                     RVALFYRLSRQHKQQRTQRMLRYYFTAQDILERASSSHYQYHELFQQLSNSDLMFRFQ
                     RVLELQAAACQKIAMSLRHREVYEHGVRGEKALQGLLNSLNYHQERGLKSAYRWRAIA
                     ENLGNIERQLAQIEQGSSSGEHIEPLSKNFTKSHRLIAENITGLSNISQAIKAHLTFE
                     SQLFRHAVRLSIVVLLCSAIVPILGFDSKGYWILLTAIFVCQPNYTATKTRLIQRVIG
                     TIFGVLVGGSFELFNVALSLEAQLGLIVLTGSLYTFFRLVHYGFSTFYITVLVMISLD
                     IAGVSLESGILMRMLDTLLGTAIAWLAVAFLWPDWKYLNLQRNLHNGLKASGQYLRHI
                     IAQLQFGYNEQLPYRIARRDVHNHLSALSNAITNMYSEPQKYANDLTFAPKLLGIAYT
                     LLGYISTLGAYRIASREINQQVDFSAMFFSHAREVANIIGQIASESRSFAKLSEKLTA
                     IDQDLTQFEQENQQKQNDLALVLIQQLRLIVQLLPQLNSILQLKSVR"
     gene            complement(2315..2773)
                     /locus_tag="PXB27_001233"
     CDS             complement(2315..2773)
                     /locus_tag="PXB27_001233"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDL5346027.1"
                     /translation="MKTTVRTIAKQKQIALVAHDSCKQDLIRWTQTHQAQLASHKLYA
                     TGTTGHLLARETGLEITSLLSGPMGGDQQLGGLIAEKKIDLLVFFWDPMNAAPHDPDV
                     KALMRIATVWNIPVAINETTANFLLNADLFNQAISVNVPDYDGYLAERLG"
     gene            2899..4173
                     /gene="thrC"
                     /locus_tag="PXB27_001234"
     CDS             2899..4173
                     /gene="thrC"
                     /locus_tag="PXB27_001234"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDL5346028.1"
                     /translation="MNLYNIKHPEEQVNFAQAVRQGLGKNQGLFFPEVLPKLDSIDEL
                     LDLPLVKRSQKILSALIGEELPQETLNAMVKNAFTFPAPLAKVNDDIYALELFHGPTL
                     AFKDFGGRFMAQALANVRGDGKITILTATSGDTGAAVAHAFYGLENINVVILYPKGKI
                     SPLQEKLFCTLGGNIRTVAIESDFDACQALVKQAFDDAELRQAIGLNSANSINISRLL
                     AQICYYFEAVAQLPKEKRDNVVVSVPSGNFGNLTAGLIAKTLGLPIKRFIASTNANDT
                     VPRYLQSGKWEPNATVATLSNAMDVSRPNNWPRVEELFKRNGWSLSELGSAALNDAET
                     EAALKAQYAEGYLCEPHGAIAYQSLKDQLQQGETGIFLCTAHPAKFKESVEHILAIEL
                     PLPEALDKHNKLPLLSDEMAADFATLRAYLLK"
     gene            complement(4252..4986)
                     /locus_tag="PXB27_001235"
     CDS             complement(4252..4986)
                     /locus_tag="PXB27_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDL5346029.1"
                     /translation="MSFKDMTGQKVPSVTFRTRQNDAWVDVTTDELFNNKTVVVFSLP
                     GAFTPTCSSTHLPRYNELACEFKALGVDDIICMSVNDTFVMNAWKADQESENITVIPD
                     GNGEFTEGMGLLVDKNDLGFGKRSWRYSMLVKNGIVEKMFIEPQEPGDPFKVSDADTM
                     IKYLSPNWTAKESVSIITKPGCLFCAKAKALLKEQGYTFEEIVLGRDASTISVRAITG
                     KTSVPQVFIGGKYIGGSDELAAYFAK"
     gene            5101..5985
                     /gene="oxyR"
                     /locus_tag="PXB27_001236"
     CDS             5101..5985
                     /gene="oxyR"
                     /locus_tag="PXB27_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HDL5346030.1"
                     /translation="MNIRDLEYLIALAEHKHFRRAAESCNVSQPTLSGQIRKLEGELG
                     TILLERTSRKVLFTQAGLTLVEQAKVVLREVKILKEMASNQGKEMSGPLHVGVIPTLG
                     PYISPIIVPALKAEFADLELYIYELQTNQLLDQLESGQLDCGIISFVKESESFIEVPI
                     FNEEMYLVVPEQHEWAALKKFDMNLLKNRELLFLDSGHCLRTNTLDYCLSIGAKENPH
                     FKATNLETLRNMVAANVGLALIPKLAAKPIKGVHYIPLENPKPFRAVGMVYRPGSPLR
                     ARYERLAQAITKIMQSEA"
     gene            5988..6620
                     /gene="fabR"
                     /locus_tag="PXB27_001237"
     CDS             5988..6620
                     /gene="fabR"
                     /locus_tag="PXB27_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HDL5346031.1"
                     /translation="MANGMSVRAIQKEKTRRALVEAAFNQLSSEKNFSNLSLREVARE
                     AGVAPTSFYRHFKDMDELGLAMVDESGLLLRQLMRQARKRIANGGSVVVVSVETFFEL
                     IADRPNVFQLLLRESSGTSQAFRTAASREIQHFVAELADYIMSKDNHSNRELAYIQSE
                     GLVTLVFTAGSHALDMNNQEREKLKQRVIMQLRMLIRGSTYYANKQASST"
     gene            complement(6647..6946)
                     /locus_tag="PXB27_001238"
     CDS             complement(6647..6946)
                     /locus_tag="PXB27_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HDL5346032.1"
                     /translation="MKKADIQKMQDCYNQWVELLPELEKGIEQWKRASALLEPLSRFY
                     ASPEWRELHDSFDEQLDTKGNYSILSEDALWNALSEHHQLALEWLKLSTAYITKE"
     gene            complement(7035..10850)
                     /locus_tag="PXB27_001239"
     CDS             complement(7035..10850)
                     /locus_tag="PXB27_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HDL5346033.1"
                     /translation="MKGSGLATVGAPETGDNGQTIYTVDVAKAGAPTVTRGNVADAIN
                     NSEKTSSVVAGSKAVTVKAGKEDDKGNTEYTVDVATDKSIGRDNDGNMTVNTDNVTIV
                     KDPTTGEVKANTTTLNNSPEGKVTEPTGDDAKKLVTAGDIANAINNSGFNVTAGDGVD
                     GGETKGKKTQLIKPSETVTFDAGKNMTLTQADGKFTYTTKDNVAFNSIDMSNGKPDTT
                     GSITNLKSGVGGTFADKAAPTDAERKAIADNINNATGDTLNNAVNVGDVQAAMKAATT
                     KVEGDKGVTVTSKTNDDGSTTYTVEAKTDGTTIKVNDKGEITANTSELTNNPDGKVEE
                     PTEPNALVTAKTVADAINNAGFNIKANGDEKSLVKTGDTVQFLNGKNIEITRDGNNIT
                     VSTAKNVNFDSVQFGNEGPKITNNGGNINVGDKDGNAVKVTNVAAGDVNADSKDAVNG
                     SQLYTFAMASREEVKSTDKSVTVNTTKNADGANVFDLSVNTDDVTIVKDPTTGAIKAN
                     TTALNDANNDGRIDEPTADDAKKLVTAGDITNAINNSGFTLKTSAVEGGEKLSGGDEL
                     INPGKAVEMVAGKNLTVKQEADGKVIYATKDDVKFSSVTSNTVTVPTDEADPANNPIT
                     INKDGINAGNKAISNVASNLIPVTADDKVQPADNNPTNLADKLSNAATLGDVLNAGWN
                     LQGNGKAVDTVVHNDTVDFINGKGTTVTVENKDGKNTIKVDSPIEFVNQDPTDSSTPS
                     NTAKFTGEAPVQLGNVASSVRNEDGSTPEGKDRAEAIKNAEGDKLNNVVNLGDLQAAT
                     NAATTKVGGNRGVTITPSTNADGSTTYNVEAKTDGTTIKVDNEGNITANTSELGNNED
                     GTVKAPTQPNALLIAQTVADAVNNAGFNIKSAGNKAAGDQAATKLVKTGEEVVFEAGD
                     NLTVKRDGNQFTFATAKDVSFNSVQFSENGPKITNDGDNIKVGDKDGKPTKITNVADG
                     DISPVSTDVINGKQLNNYAKVNGNNIGTDEDGSINIVNGNGTTITSDKAGEVKVNVNI
                     TDLTVADNGKINVQDPNGTGSRFVNATTVANAVNNVSWNVDSKAVGTGAVEGDKAPAK
                     VKAGSTVSVNAGNNIKVTRKGSDVTVAVSDTPEFTSKTGDTLVNNNGVTINNGSAGKA
                     VSLTKDGLNNGGNRITNVKAGEADTDAVNVGQLKGAVNHLNNKIHRNNREARAGIAGS
                     NAAAALPQVYIPGKSMVAAAGGTFKGENALAVGYSRSSDNGKLILKLQGNANSRGDFG
                     GGVGVGYQW"
     gene            complement(10813..11298)
                     /locus_tag="PXB27_001240"
     CDS             complement(10813..11298)
                     /locus_tag="PXB27_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346034.1"
                     /translation="MDTGSITVDNTTGAVTTPAEEDKVATTKTVSEAIQKAGWNAKSG
                     GNKADGDQEAAELINPGEKVIFAAGDNLKVKRVGTIFTYETAKDVKFDSVTFGDNGPK
                     ITNKDGNVNIAGNDGNPTKITGVKAGEADTDAVNVSQLKQAAASQNRSERFRFSNCWC
                     T"
     gene            complement(11307..13466)
                     /locus_tag="PXB27_001241"
     CDS             complement(11307..13466)
                     /locus_tag="PXB27_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL5346035.1"
                     /translation="MGARATSANAIALGTDTAATGNRATAFGAGALATGNRSTVMGWR
                     SAASGTRSFAMGSGAMGDKSVANATYSLALGSSANANATSTATRGTAIGYNATVSGVN
                     ATALGVSAVSSNQSTVAIGDSAKASAGFAIALGSGANVALNNSVALGSNSTVSNAVAV
                     PSATVNGITYSGFAANTTAAGNVVSVGSNTIKRQIQNVAAGQISATSTDAINSSQLYM
                     AMNATGNLANSTKNILGGNATVKPDGSVTYTNIGGTNKNTIEEALKAVKTEVVAGSNV
                     NITNATGANGQTIYTVNAYNTTANSSSPDYITVTGKAATAANTTNYEIGLTKKAIDDF
                     TKDTQATVVSNDGTVTVKSTERNANGTVIYDLSVNIPAQASQIQYFSVNSTVPENQAN
                     DGAKSRNSIAIGPNATATGGEQAAVALGTNSNANGNGALSLGVATVSKGIQATAVGHS
                     ANATANGTTALGRQTNATAGDATAVGSNANATAEKASAFGVAANASANASLAVGANSI
                     ASAQSAVAVGTRANATAQFATALGMGAQATLNSSVALGSESVVRAATPTENATVGGIT
                     YNGFAGVNKDTNYVVSVGSAGKERQIQNVAAGQISATSTDAINGSQLYMAMNATSNLA
                     NSTANNFGGGSVVNPDGSVTQPKYNVTNAAGTQYGNTATNVGDAITNLNNYVNQGFNI
                     KDNAGETKGTVTPNESVQFVNGKGTVSNVTQEADGVK"
     gene            complement(13695..13895)
                     /locus_tag="PXB27_001242"
     CDS             complement(13695..13895)
                     /locus_tag="PXB27_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR domain-containing protein"
                     /protein_id="HDL5346036.1"
                     /translation="MNQIFKVIWNQATQSWVAVSELQRAKGKTKSVSSASKATLALLA
                     GASAVLGANDAVAVVSYRFNNK"
BASE COUNT         3881 a         3186 c         2591 g         4268 t
ORIGIN      
        1 tcaacaaaaa attaacaaaa ccataatatt tttattgatt ttgtgaagca tgtcacattt
       61 tatgatttaa ttataggctc actaccttac actcttgagc tgtaatatac tatttaactg
      121 cggtaataac tgcacgatca gtctgagttg ttgtatcaac actaaggcga ggtcattttg
      181 cttttgctga ttttcttgct caaattgggt gaggtcttgg tcgatagcgg tcaatttttc
      241 cgataatttt gcaaatgaac ggctctctga ggcaatttgc ccgatgatgt ttgccacctc
      301 tctggcgtgt gagaagaaca ttgcggaaaa atccacttgt tgattaatct ctcggctagc
      361 aatacggtaa gcccctaagg tggaaatata acccagcaag gtgtaagcaa tacctaacaa
      421 ttttggggcg aaggttaaat cgttcgcata tttttgtggt tcgctgtaca tattggtaat
      481 cgcattactc agtgccgata aatggttatg cacatcacgg cgggcaatac ggtaaggcag
      541 ttgctcgtta taaccaaact gcaactgggc gataatatgg cgtaaatatt gcccactggc
      601 tttcaagccg ttatgtaagt tacgttgtaa atttaaatac ttccaatccg gccataagaa
      661 tgccactgcc agccacgcaa tcgctgtacc tagtaaggta tcaagcattc gcattaagat
      721 gccgctttca aggctcacgc ctgcaatatc aaggcttatc attaccaaca cagtgatata
      781 aaaggttgaa aagccataat gtaccaagcg gaaaaaggta taaagcgaac cggtcagtac
      841 gattagccca agctgggctt cgaggctgag cgccacgtta aacagttcaa aagagccacc
      901 aaccagcacg ccgaaaattg tgccgatcac acgctgaatc agtcgggtct tagtagcagt
      961 gtaattcggt tggcagacaa aaatggctgt gagtaaaatc cagtagcctt tgctatcaaa
     1021 gcctaaaatc ggcacaatcg cactgcacaa cagcaccact atcgacaacc gaacggcgtg
     1081 tcgaaacagt tgggattcaa aggttaaatg ggctttaatt gcttggctga tattgcttaa
     1141 gccggtaata ttttcggcga tcaagcggtg agattttgtg aaatttttac ttaacggttc
     1201 aatatgctca ccactgcttg agccttgttc aatctgggct agttgccgct caatattgcc
     1261 taaattttct gcaatagcac gccagcgata agcacttttc aagccccgtt cttgatgata
     1321 attcaatgaa ttaagcaagc cctgcaaggc tttttcgcca cgaacgccgt gttcatatac
     1381 ttctctatgg cgtaacgaca ttgcaatttt ttgacacgcc gcagcctgta attccagcac
     1441 ccgttgaaag cggaacatta aatcactgtt acttaattgt tggaacagtt cgtgatattg
     1501 ataatggctg gaacttgccc gctctaaaat atcttgagcg gtgaaatagt aacgcaacat
     1561 tcgctgtgtc cgttgctgct tgtgctggcg gcttaggcgg tagaataggg caactctggt
     1621 tttatcaaag gcagccatca cctcactatt ggctttcgcc aaggctagtt gtttggcagc
     1681 taaatcgtca tcatcagggt caaaataggc agatttcgcc agtaaataat ggctcaacgc
     1741 aaggtaggcg gacgctaaat tctcctgcac ggttcgattc gggaaaatca ggtacaccaa
     1801 aataccgacc aaaccataga gtaaggtgcc aagtaagatc atcaccacat tgtgatacca
     1861 caatacttcg ggcgtataag ccaggctgtt ataaaccgcc acaatcagcg tgccaaaagc
     1921 aatggtacta tagcgttgcc cgattgcccc taacattacc agcaaaaagg tgcaaaccac
     1981 cgccaccggc acaaacagcc agcccaaatg gagcgacaca ctcgccccaa tcgaggaaag
     2041 cgagaacgcc actaggctga taatcagatt tttcgctcgt ccgattaggc tgttgtctaa
     2101 atccaccaaa ccgccggcga taatgcctaa catcaacggc atcgcaaaat cagaaatacc
     2161 gagctgccaa atgctgaccg ccgcaatgtt tagcgaaata aacatcggca gggttttaat
     2221 tacattttcc gaaagccaac tgtgttgtag atatcttaaa aatttattca tatttgctca
     2281 tttcgggcag gcacaggacc tgcccctacc tacactaccc caaacgttct gccaaatagc
     2341 catcataatc cggcacattc acgctgattg cttgattgaa caaatccgca tttaacagaa
     2401 aattagcggt ggtttcgtta atcgccaccg gaatattcca aacggtcgca atccgcatca
     2461 gggctttcac atcaggatcg tgcggagcgg cattcatcgg atcccaaaag aacaccagca
     2521 gatcaatttt cttttctgcg atcagcccgc ctaattgctg atcgcccccc atcgggccgc
     2581 ttaacaatga agtaatttca agccctgttt ctctggcgag caaatgcccg gtagtgccgg
     2641 tggcatatag cttatgcgag gcaagttggg cttgatgagt ttgtgtccaa cgaattaaat
     2701 cttgtttaca gctatcgtga gccactaagg caatctgttt ttgttttgcg atggttctga
     2761 cggtggtttt catttttagc tcctttgcaa aatttttcac aaatcatacc gcttattttt
     2821 cgcttttgca ttgactaatc acggcttatg gctaaaatca ggtaaaaatt cgcattttaa
     2881 aacaaaggat taaccgcaat gaacttatac aacatcaaac accctgaaga acaagtcaat
     2941 ttcgcccaag cggtacgcca aggcttaggc aaaaatcaag ggctattttt ccctgaagtg
     3001 ttacctaaat tagatagcat tgatgaatta ttggatttac ctttggttaa acgcagccaa
     3061 aaaattcttt cagccttaat cggcgaagag ttaccgcaag aaaccttaaa tgcaatggta
     3121 aaaaatgcgt tcactttccc ggcaccgctt gccaaagtca atgatgatat ttatgcgtta
     3181 gaattatttc acggtccgac tttagccttc aaagattttg gtgggcgctt tatggctcaa
     3241 gccttagcca atgtacgagg agacggcaaa attactattt taaccgctac ctcaggtgac
     3301 accggtgcgg cggtggctca tgctttctat ggtttggaaa atatcaacgt ggtcattctt
     3361 tacccgaaag gcaaaatcag cccattacaa gaaaaattat tctgtacctt aggcggtaat
     3421 attcgcactg ttgcgattga atccgacttt gatgcttgcc aagccttagt gaaacaagcc
     3481 tttgatgatg ccgagctacg ccaagcgatt ggtttaaatt ccgcaaattc catcaacatt
     3541 agccgtttac tggctcaaat ttgctactac tttgaagcag ttgctcagtt accaaaagaa
     3601 aaacgagata acgtagtggt ttcagtacca agcggtaatt tcggtaactt aaccgccggc
     3661 ttaattgcca aaacattagg tttaccgatt aaacgtttta ttgcctcaac caatgccaat
     3721 gacaccgtgc cacgctattt acaatcaggc aaatgggaac caaacgcaac cgttgcaacc
     3781 ctttctaatg caatggacgt aagccgcccg aacaactggc cacgagttga agaattattt
     3841 aaacgcaatg gctggagttt gtctgaattg ggttctgccg cattaaacga tgccgaaacc
     3901 gaagcggctt tgaaagctca atatgcagaa ggttatctgt gtgagcctca cggtgctatt
     3961 gcttaccaaa gcttgaaaga tcagctacaa caaggcgaaa ccggtatttt cttatgcacc
     4021 gctcacccgg caaaattcaa agagtcggtg gaacacattt tagcgattga attgccgtta
     4081 ccggaagcat tagataagca caacaaactg ccactgttat ccgatgaaat ggcagcagat
     4141 tttgcaacat tgcgtgcata tttgttgaaa taaatattca ataatagaaa agggattaag
     4201 tattaaactt aatccctctt tttatgcatt acaagcggtt atatttgcct attattttgc
     4261 aaaataagca gctaactcat cactaccgcc aatatattta ccgccgataa atacttgtgg
     4321 cacagaggtt ttacctgtaa ttgctcgtac agaaattgtg cttgcatcac gacctaacac
     4381 aatctcctca aaagtatagc cttgctcttt taataatgct tttgcttttg cacagaacag
     4441 gcaacccggt ttagtgataa tcgaaacaga ctctttagct gtccaatttg gacttaaata
     4501 tttaatcatt gtatcggcat ctgatacttt aaacggatcg cccggttctt gtggctcaat
     4561 aaacattttt tccacaatac cgtttttcac cagcatcgaa taacgccaag aacgtttacc
     4621 aaacccaagg tcatttttat caactaatag ccccatacct tctgtgaact caccattacc
     4681 atcaggaata acagtaatat tttcagattc ttgatctgct ttccaagcgt tcattacaaa
     4741 ggtatcatta actgacatac agatgatatc atctacccct aatgccttaa attcacaagc
     4801 taattcatta tagcgaggca aatgtgttga tgaacaagtc ggtgtaaatg ctccgggtaa
     4861 tgaaaacaca accaccgttt tgttattaaa taactcatcg gttgtgacat caacccaagc
     4921 atcgttttga cgtgtacgaa atgttacact gggtactttc tgacccgtca tatctttaaa
     4981 agacatatta atgttctcct atagaattat atattacaaa aatcttaggc attatactca
     5041 caaatatggc ataattcagc ctatcagaac aataggaaaa atttaaattg gaggaatcaa
     5101 gtgaatattc gagatttaga ataccttatt gcacttgcag aacataagca tttccgtcgt
     5161 gcagcagaat cttgtaatgt tagccaacct actttaagcg ggcaaattcg caaattagaa
     5221 ggcgaactag gcactattct gcttgaaaga accagtcgca aagtcctctt tacgcaagca
     5281 ggcttaacat tagttgaaca agccaaggta gttttacgtg aagtaaaaat tctaaaagaa
     5341 atggcaagca accaaggtaa agagatgtct ggcccgcttc atgttggtgt aatcccaacc
     5401 ttggggcctt acatttcgcc aattattgtg cctgcattaa aagcagaatt tgcagattta
     5461 gagctgtata tttacgaatt acaaaccaac caattattag atcaacttga atccggccag
     5521 cttgattgtg gcattatctc atttgtaaaa gagagtgaat cttttattga agtgcctatc
     5581 tttaatgaag aaatgtactt ggtagttcca gaacaacacg aatgggcagc gcttaaaaaa
     5641 ttcgatatga atttattaaa aaaccgtgag ttgctctttt tagacagtgg ccattgttta
     5701 cgcactaata cgctagacta ttgtctatcc attggagcaa aagaaaatcc acactttaaa
     5761 gctaccaacc ttgaaacctt acgcaatatg gtagcagcaa atgttggcct agcattgatt
     5821 cctaaattag ccgctaaacc gattaaagga gtacattata ttccacttga aaacccgaaa
     5881 ccgtttcgag ctgtgggaat ggtgtatcga ccaggttcac cattacgcgc ccgctatgag
     5941 cgtttagctc aagccattac taaaattatg caatccgagg cgtaaaaatg gcaaacggta
     6001 tgagtgtgcg cgcaattcag aaagaaaaaa cacgcagagc attagtagaa gccgccttta
     6061 atcaattaag ctcagaaaaa aatttctcta atctcagttt gcgtgaagtt gcacgtgaag
     6121 ctggagtcgc accgacttcg ttttaccgcc actttaaaga tatggacgaa ctaggcttag
     6181 caatggtgga cgaatccggg ctactattac gccaattaat gaggcaagct cgaaaacgaa
     6241 ttgcaaatgg cggaagtgtt gtcgtggttt cagtggaaac attcttcgaa ctgattgccg
     6301 accgccctaa cgttttccag ctattactac gcgaaagctc cggtacatcg caagcgttta
     6361 gaaccgcagc atcacgcgaa attcaacatt ttgtagctga attggcggac tatattatga
     6421 gcaaagataa tcacagtaac cgagaacttg cttatattca atcggaaggt ttagtcacgc
     6481 tagtatttac tgccggttct catgctttgg atatgaacaa tcaagaacgg gaaaaactaa
     6541 agcaacgtgt cattatgcag ctaagaatgc tgattcgtgg ctccacctat tatgccaata
     6601 aacaagcttc atcaacataa aaaaacagac gcctaagcgt ctgtttttat tctttcgtaa
     6661 tatatgcagt cgatagcttt aaccattcta gtgctaattg atgatgttca gataacgcat
     6721 tccataacgc atcttctgac aagatactat aattgccttt agtatctaac tgttcatcaa
     6781 aactatcatg taattcacgc cattccgggc tagcataaaa ccgacttaag ggttccaaaa
     6841 gtgcagaagc tcgtttccat tgctcaatcc ccttttccaa ttctggtaat agctctaccc
     6901 attggttata acaatcctgc attttctgaa tatctgcttt tttcattctt taccgcttac
     6961 accataaatt ttagcctcaa tataacataa aaatcccctt ccgaagaagg ggatttcaac
     7021 tcaattaggc taaattacca ctggtatcca acgcctacac cgccaccaaa gtcgcctcgg
     7081 ctattcgcat taccttgtaa tttcaagata agcttaccgt tatcacttga acgtgaataa
     7141 cctactgcaa gcgcgttttc acctttaaat gtaccacctg ctgcagccac catagattta
     7201 cctggaatgt aaacctgcgg taacgctgct gctgcatttg aaccggcaat accagcacgg
     7261 gcttcacggt tattacgatg gatcttgtta tttaagtgat taacagcacc ttttaactga
     7321 ccaacattaa cagcatcggt atctgcctca cctgctttaa cattagtaat acggttacca
     7381 ccattattta agccatcttt cgttaagctt accgcttttc ctgcagagcc attattaatg
     7441 gttacgccat tattgttcac caatgtatca ccagtcttgc tagtaaattc aggggtatct
     7501 gaaacggcta cagttacatc agaaccttta cgtgtcactt tgatattatt acctgcattc
     7561 acgctcactg tactacctgc ttttactttc gcaggggctt tatccccttc aaccgcacct
     7621 gtaccaactg ctttagaatc tacgttccag cttacgttgt ttacggcatt tgctactgtt
     7681 gttgcattca caaagcgtga accggtaccg ttaggatctt gaacattgat tttgccgtta
     7741 tcagcaacgg ttaaatctgt gatgtttacg ttcactttca cctcaccagc cttatcactg
     7801 gtaatagttg taccattacc atttacgata ttgatagaac catcttcatc agtaccaatg
     7861 ttattaccat tcactttcgc atagttgttc agctgtttac cgttgataac atctgtgctt
     7921 actgggctaa tatcgccatc tgctacatta gtgatcttag ttggtttacc gtctttatca
     7981 ccgactttga tgttatcacc atcattagtg attttcggac cattctcact gaactgtacg
     8041 ctattgaagc tcacatcttt tgctgtcgca aaagtaaatt gattaccatc acgtttaaca
     8101 gttaagttat cacctgcttc aaacacaact tcttcgcccg ttttcactag cttagtcgca
     8161 gcttggtcac cggcagcctt attacctgca gatttaatat taaagcctgc attattcact
     8221 gcatctgcaa cagtttgagc aatcaataac gcatttggct gagttggtgc tttcaccgta
     8281 ccatcttcgt tgttacctaa ctcactggta tttgcagtga tgttaccttc attatctact
     8341 ttaatagtag taccgtcagt tttcgcttca acgttataag tagttgaacc atcagcattc
     8401 gtgctaggtg tgatagttac acctcggtta ccgcccactt tcgtagtagc tgcattagtt
     8461 gccgcttgta aatcacctaa gttcactacg ttgtttagct tgtcgccttc agcattttta
     8521 atcgcttctg cacggtcttt accctctggg gttgagccat cttcgttacg aacactactt
     8581 gccacattgc ctaattgaac aggggcttca cctgtgaatt tagccgtatt gcttggcgtt
     8641 gagctatcag taggatcttg gtttacaaac tcaatcgggc tatctacttt aatcgtattc
     8701 ttaccgtctt tgttttcaac cgtgaccgtt gtacctttac cgttaataaa atcaaccgtg
     8761 tcattatgta ccacagtatc aaccgctttg ccgttacctt gtaaattcca gcctgcattt
     8821 aatacatcac ctaatgttgc tgcattactt aacttatcag ctaagttggt tggattattg
     8881 tctgctggtt gaaccttatc atctgcagtc acaggtatca gattagacgc aacattgcta
     8941 atcgctttat tacctgcatt gataccatct ttattgatag taattggatt atttgcagga
     9001 tctgcttcat cagttggtac agttacagtg ttagaggtta cgctgctaaa cttaacatca
     9061 tctttagtcg cataaatgac tttaccatca gcttcttgct tcacagttaa gtttttacct
     9121 gcaaccattt caactgcttt acccggatta atcagttcat caccacctga aagcttctct
     9181 ccaccttcta cagccgacgt tttcaacgta aagcctgagt tattgatggc gttggtaata
     9241 tcacctgctg tcactaattt cttagcatca tcagcggttg gttcatcaat tctaccgtca
     9301 ttatttgcat catttaatgc cgtagtgtta gccttgatcg caccggttgt aggatctttt
     9361 acaattgtta catcgtccgt attcacagaa agatcgaaca cattcgcgcc atcagcattt
     9421 tttgtcgtgt taaccgtaac tgatttatca gtcgacttca cttcttcacg agatgccata
     9481 gcgaaagtat aaagctgact accatttact gcatctttac tatctgcatt tacgtcaccg
     9541 gcagctacat tagttacttt gactgcatta ccgtctttgt caccaacatt gatattacca
     9601 ccattgttag taatcttagg accttcattt ccgaactgta cactatcaaa attcacattt
     9661 tttgcagttg aaacagtaat gttattccca tcacgggtaa tctcaatgtt tttaccattt
     9721 aagaactgaa ccgtatcacc tgttttcacc aggctcttct cgtcaccgtt tgctttaatg
     9781 ttaaaacctg cattgttaat tgcatctgca acggttttag ctgttactaa tgcatttggt
     9841 tctgtaggct cttccacttt accatcaggg ttatttgtta actcgctggt gtttgccgtg
     9901 atttcgccct tatcgtttac cttaatggtt gtaccatccg tttttgcttc aacggtataa
     9961 gtggttgagc cgtcatcgtt agttttagat gttacagtaa cacctttatc accttccact
    10021 ttggtggttg ctgctttcat tgctgcttgt acatcaccca cattcacagc attattcaac
    10081 gtatcacctg ttgcgttgtt aatattatcc gcaatcgctt tacgctctgc atctgttggt
    10141 gctgctttat ctgcaaatgt accacccacg ccgctcttca gattagtgat tgagcctgtt
    10201 gtatctggtt taccattgct catatcaatg ctgttgaatg caacattatc tttcgttgtg
    10261 taagtgaatt taccgtcagc ttgggtaagt gtcatatttt tacccgcatc aaaggtaact
    10321 gtttcacttg gcttgattag ctgtgttttc ttacctttgg tttcaccacc atcaacacca
    10381 tcacctgctg ttacattaaa gcctgaatta ttgattgcat tagcaatatc acctgccgtc
    10441 actaatttct tagcatcgtc acctgtaggc tcagtaactt taccttcagg agaattattt
    10501 aatgttgtgg tattcgcttt aacttcgcct gttgtcggat ctttcacgat agtaacgttg
    10561 tcagtattca ctgtcatatt accgtcatta tcacgaccga tagatttatc cgttgcaaca
    10621 tctaccgtat attcagtatt gcctttatca tcttcctttc cggctttaac agttacggct
    10681 ttagaaccag ctactacaga tgaggttttc tctgaattat taattgcatc agccacatta
    10741 ccacgagtta ctgtaggagc acctgctttc gctacatcta ccgtatagat tgtttgacca
    10801 ttatcacctg tttcaggtgc accaacagtt gctaaacctg aacctttcac ttcggttttg
    10861 tgacgctgct gcttgtttta actgacttac attcaccgca tcagtatcag cttcacctgc
    10921 tttcacgcct gtaattttgg ttggattacc atcattacct gcaatattaa cattaccatc
    10981 tttattcgtg attttcgggc cattatcacc aaatgttacg ctatcaaatt tcacatcttt
    11041 cgctgtttcg tacgtgaata tagtgccaac acgtttaact tttaagttat cgcctgctgc
    11101 aaaaatcact ttttcgcccg gattaatcaa ctctgctgct tcttgatcac catcagcttt
    11161 attgccacca gattttgcat tccaacctgc tttttgaatc gcttcagaaa ctgtttttgt
    11221 agttgcaact ttatcttctt ctgcaggtgt agttactgca cctgttgtgt tatcaaccgt
    11281 gatagaaccg gtatccacat caaaggttac tttacgccat cagcttcttg tgtaacattt
    11341 gaaactgtac ctttaccgtt tacaaattgt acagattcat ttggggtcac tgtgcctttg
    11401 gtttcacccg cattatcttt aatattgaaa ccttggttta catagttatt taagttcgta
    11461 attgcatcgc caacgtttgt tgcagtatta ccatactgag ttccagctgc attcgttaca
    11521 ttatatttag gctgagtcac agagccatcc ggattcacaa cagaaccgcc accaaaatta
    11581 tttgcagttg aatttgcaag gttactggtt gcattcattg ccatataaag ttgtgaacca
    11641 ttaatcgcat cggttgatgt agcagaaatc tgacccgctg ccacattttg gatttgacgc
    11701 tctttacctg cagagcctac agaaacaaca tagttagtat ctttatttac cccggcaaag
    11761 ccattataag taataccccc taccgttgca ttttcagttg gtgttgctgc ccttacgact
    11821 gattcgctac ccaaagcaac agaactattc agagtagctt gagctcccat acctaatgct
    11881 gtagcaaatt gagctgttgc attagcacgt gtaccaacag ccactgctga ttgtgctgag
    11941 gcaattgaat ttgcacctac ggctaaagag gcatttgcac tcgcatttgc tgcaacacca
    12001 aaggcagaag ctttttcagc cgttgcatta gcattagaac ctaccgctgt agcatcacct
    12061 gctgtcgcat ttgtttggcg accaagtgcg gttgtaccat tagccgttgc atttgctgaa
    12121 tgaccaactg ccgtagcttg aatacctttt gaaaccgtag caacacctaa agataacgct
    12181 ccgttaccat ttgcatttga gtttgtgcct aatgctactg ccgcttgctc tccacctgtt
    12241 gccgttgcat tcgggccgat cgcaattgag tttctgctct tcgcgccatc attagcttga
    12301 ttctcaggta cggttgaatt cacagagaaa tattgaattt ggcttgcttg cgcaggaata
    12361 ttgacagaaa gatcataaat aaccgtgcca tttgcatttc tctctgtact ttttacagtt
    12421 actgtaccat cgtttgaaac aacagtagct tgggtatctt tcgtaaagtc atcaatggct
    12481 ttcttagtta aaccaatttc atagttagtt gtattcgcag cagttgcagc tttacctgtt
    12541 acagtaatgt aatcagggct agaagaattt gccgtggtgt tatacgcatt tactgtataa
    12601 atagtttgcc catttgcacc ggttgcattt gtaatattaa cattgctacc tgcaacaact
    12661 tctgttttta ccgcttttaa tgcttcttca atcgtatttt tatttgttcc accaatatta
    12721 gtataggtaa cagaaccatc aggctttact gtagcattac cacctaaaat atttttagtt
    12781 gaattagcaa ggttacctgt tgcgttcatt gccatataaa gctgtgaact attaattgca
    12841 tcggttgatg tagcagaaat ctgacccgct gccacatttt ggatttggcg tttgatcgtg
    12901 tttgagccaa cagaaacaac attccctgct gccgtagtat tagccgcaaa gccggaataa
    12961 gtaataccat ttaccgtcgc agaaggaact gccacagcgt tggaaacggt tgaattactc
    13021 cctaaagcaa ccgaattatt tagcgctaca ttagcacctg agcctaaggc aatagcaaaa
    13081 ccggcacttg ctttagcact atcaccaatt gcaaccgtag attggttgga agatactgca
    13141 cttaccccta aagctgtcgc attaacacct gaaactgtcg cattataacc aatcgctgta
    13201 cctctagttg cagtagaagt tgcattagca ttagcacttg aacctaaagc taaagaataa
    13261 gtagcatttg caacagactt atcccccatt gcacctgaac ccattgcaaa tgaacgagtg
    13321 ccactagcgg cagatctcca tcccattact gttgaacggt taccagtagc tagagctcct
    13381 gcaccaaaag ctgttgcacg gttacctgtt gcagcagtat ccgtacctaa tgcaatagca
    13441 tttgctgaag tcgctctagc ccccatacca atagcaatag aaggaactaa tgcccaattc
    13501 tgaccagtgt tattgagact ctctacacca atattatagt aatgtaagaa gtatcttctg
    13561 gatccactaa atttgttcta tgcgttgcat tcgtatctgt accaattgca atagtagatc
    13621 tgccagttga gattgaatcc gcaccaatag caattgcgtt ctccgatgat gcagttgctt
    13681 gtgttgaaat ataattactt gttgttgaac ctataagata caacagccac agcatcatta
    13741 gctcccaaca cggcactagc tccagccaat aaagccaatg ttgccttact tgcagaacta
    13801 acagatttcg ttttaccttt cgctcgttgt aattcagata ctgcaaccca actttgtgtt
    13861 gcctgattcc aaataacttt aaaaatttga ttcataatcg accctatata aaaataaaaa
    13921 agatta
//
LOCUS       DANLUQ010000053        13885 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.053, whole genome shotgun sequence.
ACCESSION   DANLUQ010000053 DANLUQ010000000
VERSION     DANLUQ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13885
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.053"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(89..1024)
                     /gene="ftsX"
                     /locus_tag="PXB27_001243"
     CDS             complement(89..1024)
                     /gene="ftsX"
                     /locus_tag="PXB27_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HDL5346037.1"
                     /translation="MIRSFSKLGAQTNYSLRAVWQDLMQRKLGTFLTVLVIAVSLTIP
                     TVSYLLWKNTHEAATQFYPEPQLTVYLHKNLAEHDVNTVVDRIRAFETDKIESFNYIS
                     RQQSLDEFRSWSGFGEALDILDENPLPAVVTLKPKANFNNADTMVELRNGLQQIKGVQ
                     EVRLDNGWLEKLTALTWLIARIAVVCTLLMLLAVFLVIGNSVRTDVANSKAAIEVQQI
                     LGATDHFIARPFLYTGMIYGLFGSLLAVLFSAVTISYFTGVVKYVTDMFTVQFELNGL
                     DFSEAFFLVVLCVFIGWLSAKIATMRNIFRIGSRG"
     gene            complement(1034..1687)
                     /gene="ftsE"
                     /locus_tag="PXB27_001244"
     CDS             complement(1034..1687)
                     /gene="ftsE"
                     /locus_tag="PXB27_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HDL5346038.1"
                     /translation="MIKFTNVNKVYKGGKPALQGIDFHLPAGGMAYITGHSGAGKSTL
                     LKLIMGIERANGGQILFNGHDITRLAHHELPFLRRQIGMVHQDYRLLTDRTILDNVAL
                     PLIILGMNQAIVEREARIALERVGLADKANYLPLHLSGGEQQRVDIARAIVHRPTLLL
                     ADEPTGNLDEKLSFEIFRLFEEFNQAGTTVLIATHDTNIISKRPKPCLVLEQGHLKF"
     gene            complement(1680..3176)
                     /gene="ftsY"
                     /locus_tag="PXB27_001245"
     CDS             complement(1680..3176)
                     /gene="ftsY"
                     /locus_tag="PXB27_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDL5346039.1"
                     /translation="MSEKKKGFWSWLGFGKKEEAEQKEIQQEQESIQPEQPVIVEPET
                     EKSFSEKLDELEDKIEEKVDQLEDYFEEKEEKIEQFAEQVEDTIEQKVEDFKEFVEEK
                     VEEAKAFFDDVEDKIEQTFEEQKGEQAVENLQNIATEQLDLNEQEQVTYQVEEAVAEE
                     VAETELVNNVQDTVEESTIQDEYQEKPSQGGFFSRLIQGLIKTKQNIGSGFRNFFRGK
                     KIDDELFEELEEQLLVADLGMPTTQKIINNLTQHATKQQLKDADLLYQQLKVELGEVL
                     KPVAQPLVLEAKKPYVILMVGVNGVGKTTTIGKLARKFQQEGKSVMLAAGDTFRAAAV
                     EQLQVWGERNNIPVVAQSTGSDSASVIFDAMQSAAAKGIDVLIADTAGRLQNKNNLMD
                     ELKKIVRVMKKYDETAPHEIMLTLDAGTGQNAISQAKLFNEAVGLTGITLTKLDGTAK
                     GGVIFAIADQFNIPIRFIGVGEKIEDLRPFNAEEFIEALFSHEDEQND"
     gene            3255..3845
                     /gene="rsmD"
                     /locus_tag="PXB27_001246"
     CDS             3255..3845
                     /gene="rsmD"
                     /locus_tag="PXB27_001246"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDL5346040.1"
                     /translation="MKKNRNSPQSVQQMGEVRVIAGLWRGRKLPVLNAEGLRPTTDRV
                     KETLFNWLMHDIAHSRCLDCFAGSGSLGIEALSRQAQAVVFLEKFANAANQLKKNLQA
                     LKSEQGSVIHTDTLAYLAQKNNGEPFNIVFIDPPFHQGFVPQVLNLLAENNWLAPNAI
                     LYVETEKNHPPLELPQGWQILKEKAAGMVVSRLIQL"
     gene            3929..4243
                     /locus_tag="PXB27_001247"
     CDS             3929..4243
                     /locus_tag="PXB27_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HDL5346041.1"
                     /translation="MKKLTPDEAIDIAYDIFLEMAGENLDPADILLFNLQFEERGAVE
                     MVETSEDWDQEIGTLIDPDAFAEVWVGLVNEQDEMDDVFARFLISHDEENREYHVVWK
                     EK"
     gene            4256..4768
                     /gene="hpaC"
                     /locus_tag="PXB27_001248"
     CDS             4256..4768
                     /gene="hpaC"
                     /locus_tag="PXB27_001248"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HDL5346042.1"
                     /translation="MANIDTNQPIEASLKFREAMAHLSSAVSIVTTNGVAGKAGLTVS
                     SVCSVTDSPPTLLFCINKNSHVHDIIQQNGKVCVNVLSHEQEDLAKHFAAILESTMEE
                     RFGWDIWNNGFDDQPVLRSAISALQGNIVDTHAVGTHTIFVVEVSHIDVEPNHSLVYF
                     ARQFRSVNID"
     gene            4886..5758
                     /gene="tehB"
                     /locus_tag="PXB27_001249"
     CDS             4886..5758
                     /gene="tehB"
                     /locus_tag="PXB27_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HDL5346043.1"
                     /translation="MENLICYKQMPVWTKATLPQMFQEKHNTKVGTWAKLTILKGSLK
                     YYELTEEGEIIKEDIFDANHQRPMVEPQLWHKVEALSDDLECQLAFYCKPEDYFAKKY
                     NLTATHSEVLNAVNYTKGGKALDLGCGRGRNALYLNLLGFDVTAVDHNQESIDFLNYM
                     IEKEQAQNITTAVYDINEATIGSQNGEYDLIISTVVMMFLNRERIPAIIENMQKNTKV
                     GGYNLIVCAMSTDDYPCPMPFSFTFKEGELADYYQGWELVKYNEDLGHLHKTDENGNR
                     IQLRFATMLAKKIS"
     gene            5896..6933
                     /gene="purM"
                     /locus_tag="PXB27_001250"
     CDS             5896..6933
                     /gene="purM"
                     /locus_tag="PXB27_001250"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011962107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDL5346044.1"
                     /translation="MSNTQLSYKDAGVDIHAGNELVERIKPDVKRTRRPEVMGGLGGF
                     GALCALPTKYKEPILVSGTDGVGTKLRLAIDLNKHDTIGQDLVAMCVNDLVVQGAEPL
                     FFLDYYATGKLEVDVATNVVKGIADGCVISGCALVGGETAEMPGMYHEGDYDLAGFCV
                     GVVEKSKIIDGSTVKTGDALIALASSGPHSNGYSLIRKVLEVSRSNPATDLLEDKPLS
                     EHLLAPTKIYVKSILKLVEEVDVHAIAHLTGGGFWENIPRVLPESAKAVIDESSWQWP
                     AIFSWLQEKGNISRYEMYRTFNCGVGMVIALPERDVEKALTLLKESGENAWLIGKIED
                     LGTGSEQVEIR"
     gene            7011..7289
                     /locus_tag="PXB27_001251"
     CDS             7011..7289
                     /locus_tag="PXB27_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HDL5346045.1"
                     /translation="MQLFWPIYFVLMNLAALYLMYSDKQKAIKKEWRVPEITLLSFCL
                     LGGAIGVYLGMKYFRHKTQHWYFHVAVVLSTFAWLIGLPYFYLALSSY"
     gene            7432..8859
                     /gene="aspA"
                     /locus_tag="PXB27_001252"
     CDS             7432..8859
                     /gene="aspA"
                     /locus_tag="PXB27_001252"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HDL5346046.1"
                     /translation="MSDVRIEHDLLGEREVPNSAYWGIHTLRAIENFNISNVTISDVP
                     EFVRGMVMVKKATALANGELGAIPKKIAKAIVAACDEVLVKGRCLDQFPSDVYQGGAG
                     TSVNMNTNEVIANLALEILGEAKGKYDIVNPMDHVNASQSTNDAYPTGFRIAVYNSIT
                     KLINKITYLQTAFENKATEFSDVLKMGRTQLQDAVPMTLGQEFKAFAVLLDEEVRNLK
                     RTAELLLEVNLGATAIGTGLNTPEGYSTLAVKYLAEVTQLPCSLSENLIEATSDCGAY
                     VMVHGALKRTAVKLSKVCNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPEV
                     VNQVCFKVIGNDTTVTFAAEAGQLQLNVMEPVIGQAMFESIELLSNACINLRDKCIDG
                     ITANRDICQNYVFSSIGIVTYLNPFIGHHNGDIVGKICATTGKSVREVVLERGLLTEE
                     QLDDILSIENLMNPTYKAKRFTEEE"
     gene            complement(8934..10952)
                     /gene="rep"
                     /locus_tag="PXB27_001253"
     CDS             complement(8934..10952)
                     /gene="rep"
                     /locus_tag="PXB27_001253"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HDL5346047.1"
                     /translation="MKLNSQQQQAVEYTKGACLVLAGAGSGKTRVIINKIAHLIAHCG
                     YSPKQIAAVTFTNKAAREMRERVDHSIGKENSKGLTISTFHTLGFEILKREYKLLGFK
                     SGMTLFDEQDQIALLKHLLPENVTEDKDLLKALIATISNWKNDLLSPERVLTRVRDER
                     ERVFSHFYQLYQNQLKAYNALDFDDLIMLPVLLFRQFPEAKSRWQQKIRYLLVDEYQD
                     TNTSQYELIKLIVGNEPNFTVVGDDDQSIYSWRGAKPENMQRLREDFDLQVIKLEQNY
                     RSSQRILHCANILIDNNDHLFSKRLFSELGQGEKLQVIEAKNEEHEAERIVGELIAHR
                     FSKKTKYKDYAILYRGNHQSRLLEKLLMQNRIPYKISGGTSFFARAEIKDMMAYLRLV
                     VNQDDDAAFLRIVNTPKREIGAATLEKLGILANEKQVSLFEAIFDFELIQRLTPKPYQ
                     ALQDFARWIVEIADQADRSDPIEAVKDLLAKIQYEAYLYEMAANPKVAEMQSRNVQTL
                     FEWVQAMLEGDEIEPPMNLVQVVNRLTLRDMLERGEEDDEADQVQLMTLHASKGLEFP
                     HVFLIGMEEGILPHQTSLDEDNVEEERRLAYVGITRAQQTLTFSLCKERRQYGEIIKP
                     EPSRFLLELPQDDLEWESEKPRMTEQEKEAKSSANIANLMAMLKANKK"
     gene            11200..12315
                     /gene="potA"
                     /locus_tag="PXB27_001254"
     CDS             11200..12315
                     /gene="potA"
                     /locus_tag="PXB27_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HDL5346048.1"
                     /translation="MEKVEQKAIIELKNVTKSYGSKTILKNLNLTINDGEFLTILGPS
                     GCGKTTALRLIAGFEDLTEGSIILDGQDVSNVPAEQRPVNTVFQSYALFPHMTIFENV
                     AFGLRMQKVPNAEIEPRVMEALRMVRLEDRAQQKPAQLSGGQQQRIAIARAVVNKPKV
                     LLLDESLSALDYKLRKEMQNELKALQRQLGITFIFVTHDQEEALTMSDRIIVMNAGKI
                     AQDGTPREIYEEPSNLFVARFIGEINVFDATVIERVDATNVKANVEGRICNIKVEDLE
                     VQPNQKLKVLLRPEDIVIEELDENESSKAIIGHIIDRNYKGMTLESSVRLDHNGMNVL
                     VSEFFNEDDPNIDHEVGQKVALTWYEGWEVVLNDEGE"
     gene            12302..13165
                     /gene="potB"
                     /locus_tag="PXB27_001255"
     CDS             12302..13165
                     /gene="potB"
                     /locus_tag="PXB27_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005698572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HDL5346049.1"
                     /translation="MKANNKFQNGAVATIFGWLLLFVLVPNVLVLIISFLTENRQSQY
                     FVDFAFSFDAYKSLFNDTYAAVLWNSLYMAGIATFFCLIIGYPFAFFIAKMPERIRPF
                     LLFLVVLPFWTNSLIRIYGIKIFLGVKGFLNESLMALGIISEPMRLLNSEIAIIIGLV
                     YILLPFMILPLYSSIEKIDNRLLEAAKDLGANAFQRFIKVTIPLTMPGIVAGCLLVLL
                     PAMGMFYVADLLGGGKTPLVGNIIKSLFLNTNQFALGSAVSIALTILMALMLYVYYRA
                     NKLLNKKVELE"
     gene            13165..>13885
                     /gene="potC"
                     /locus_tag="PXB27_001256"
     CDS             13165..>13885
                     /gene="potC"
                     /locus_tag="PXB27_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HDL5346050.1"
                     /translation="MKKMFRNLFMLVVFAYLYIPIIILVINSFNEDRFGLNWKGFTWN
                     WYERLFSNDTLVQATLNSLTIAFFAATFSTILGALTSIALYRYRFRGKKLVGGMLFVV
                     MMSPDIVMAVSMLVLFMILGIKLGFISLLIAHITFCLPYVVIAISSRLSDFDGKMLEA
                     AKDLGASEVTILRKIILPLALPSIISGWLLSFTISLDDVIVSSFVTSPSYEVLPLKIF
                     SLVKTGVTPEVNALATIMIIFS"
BASE COUNT         4043 a         2915 c         2717 g         4210 t
ORIGIN      
        1 agcaataata tattgcttat agtaaaaaac caagttccta ggaactggtt tttatctgca
       61 attatttctc taaaaacaac cgcttgtatt agcctctact accaatacgg aaaatatttc
      121 gcattgtcgc aatttttgcc gacagccagc caataaacac acaaagcacc actaagaaga
      181 aggcttcgct gaaatctaat ccgttcagtt caaattgtac ggtaaacata tcagtcacat
      241 acttcactac accggtgaaa tagctaatcg ttaccgcact aaacagcacc gctaacaggc
      301 tgccaaacaa gccgtaaatc atacctgtgt ataaaaacgg acgggcaata aagtgatcag
      361 ttgcccctaa aatttgttgg acttcaatcg ctgctttgct attggcaaca tcggttcgca
      421 cactgtttcc aatcactaaa aaaactgcca acagcattaa taaggtacac accaccgcaa
      481 tacgagcaat cagccaagtt agagcagtta atttttccag ccagccgtta tctaaacgaa
      541 cctcctgcac ccctttaatt tgttgcaaac cgttgcgtag ctccaccatc gtatcagcat
      601 tattaaaatt tgctttcggt tttaaggtaa cgaccgcagg caacgggttt tcatctaaaa
      661 tatctaacgc ttcaccaaag cctgaccaag aacgaaattc gtctaaactt tgttggcgag
      721 aaatatagtt aaatgactca attttatccg tctcaaaagc acgaatgcga tcaacaacgg
      781 tattcacatc gtgttctgcc aaatttttgt gcagataaac ggttaattgt ggctcagggt
      841 aaaattgtgt tgctgcctcg tgggtattct tccaaagtaa ataactcacg gttggaatcg
      901 taagtgagac cgcaatcacc agcaccgtca gaaaagtgcc gagcttgcgt tgcattaaat
      961 cttgccaaac cgctcttaaa gagtagttgg tttgagcacc taatttacta aatgaacgaa
     1021 tcataatatt tccttaaaat ttaagatgcc cttgctccag cactaaacac ggctttggac
     1081 gctttgaaat aatattggta tcgtgggtgg caatcagcac cgttgtgccg gcttgattaa
     1141 attcctcaaa caaacggaaa atttcaaacg agagcttttc atctaagttt ccggttggct
     1201 catcggctaa taacagcgtt gggcgatgaa caatggccct ggcaatatcg acacgttgtt
     1261 gctcaccgcc ggaaaggtgc aacggcagat agttcgcttt atctgccaag ccaacacgct
     1321 caagggcaat gcgggcttca cgctccacaa tcgcttggtt cattcccaaa ataatcaatg
     1381 gtaatgcgac attatccaaa atggttcggt cggttaataa gcgataatct tgatgcacca
     1441 tcccgatttg gcggcgtaaa aacggcaatt catgatgagc aagtcgggta atatcgtgtc
     1501 cgttaaacaa aatttgcccg ccattggcac gctcaatccc cataattaat tttaatagcg
     1561 tgcttttccc cgcaccggaa tgcccggtaa tatatgccat tccaccggca ggtaggtgga
     1621 aatcaatgcc ctgtaaggct ggctttccgc ccttataaac cttgttcaca ttagtgaatt
     1681 taatcattct gttcgtcctc gtggctaaat aaggcttcaa taaattcttc tgcattaaac
     1741 gggcgtagat cttcaatttt ctcgcccacc ccaataaagc ggatcggaat attgaattga
     1801 tcggcaatcg caaaaatcac gccacctttt gccgtaccgt ctaatttagt taaggtgata
     1861 ccggttaaac ccactgcctc attaaacaat ttcgcttggc tgatcgcatt ttgcccagtg
     1921 ccggcatcaa gcgtaagcat aatttcgtgc ggtgcggttt catcatattt tttcatcacc
     1981 cgcacaattt tcttcaactc gtccatcagg ttatttttgt tttgcaaacg ccctgcagta
     2041 tcagcgatta gcacgtcaat cccttttgcc gcagcggatt gcatcgcatc aaaaatcacc
     2101 gaggcagaat ccgaaccggt gctttgtgcc accaccggaa tgttatttcg ctctccccaa
     2161 acctgtagct gttcaactgc cgccgctcgg aacgtgtctc ctgctgctaa cattaccgat
     2221 ttgccttctt gttggaattt acgggcaagt ttgccaatgg tagtggtttt ccccacgcca
     2281 ttcacaccaa ccattaaaat gacatacggt tttttcgctt ccaacactaa cggttgagcc
     2341 accggtttta gcacttcacc cagttccact ttcaactgtt gatacaacaa atctgcatct
     2401 tttaattgtt gcttggtagc atgttgcgtt aaattgttga tgattttttg tgtggtcggc
     2461 atacctaaat ccgccacaag caactgctct tccagctctt caaataactc atcatcaatt
     2521 ttcttgccac ggaagaaatt acggaagccc gaaccaatat tctgcttggt tttaatcaag
     2581 ccttgaatta aacgactgaa gaaaccgcct tgactcggtt tttcttgata ttcatcttgg
     2641 atagtagatt cttcaactgt gtcttgaaca ttatttacta attcagtttc agcaacttct
     2701 tcagccacag cctcttcgac ttgataagta acttgctctt gttcgttgag atctaactgc
     2761 tcagttgcaa tattttgaag attttcgacc gcttgttcgc ctttttgttc ttcaaatgtt
     2821 tgttcgattt tatcttcaac atcatcaaag aacgctttcg cctcttcaac tttttcttca
     2881 acaaactctt tgaaatcttc gactttttgt tcaatggtat cttctacttg ctcagcaaat
     2941 tgctcaattt tttcttcttt ttcctcaaaa tagtcttcta actgatctac cttttcttca
     3001 attttatctt ccagctcatc taatttttca gaaaacgact tctcagtttc cggttcaaca
     3061 ataacaggct gttccggttg aatactctct tgttcttgtt gaatctcttt ctgttcagct
     3121 tcttctttct taccaaatcc aagccaagac caaaaccctt ttttcttctc tgacatattt
     3181 ttctctcatt tctcgtaaaa atagactaaa cttacatagt ctatcaaaaa atcccttatt
     3241 ttcctttagt gagtatgaaa aaaaatcgaa attcccccca atctgttcag caaatgggcg
     3301 aagtacgggt tatcgccgga ctgtggcgag gcagaaaatt acctgtacta aatgccgagg
     3361 ggcttcgccc gacaaccgat agagtaaaag aaacgctgtt taactggtta atgcacgaca
     3421 ttgctcactc acgctgtctt gattgctttg caggcagtgg ctctctgggc attgaagccc
     3481 tttcccgcca agcacaagcg gtcgtttttt tggaaaaatt tgcaaatgct gccaaccagt
     3541 taaagaaaaa tctccaagca ttaaaatccg aacaaggtag cgttattcac actgacaccc
     3601 tcgcctattt agcacaaaaa aataacggtg agccatttaa catcgtattt atcgatccgc
     3661 cttttcatca agggtttgta cctcaagtgc taaacttgct ggcagaaaat aactggctcg
     3721 ccccgaatgc gattttgtat gtggaaaccg agaaaaatca cccgccatta gagctaccgc
     3781 aaggctggca aattttaaag gaaaaagcag ccggaatggt ggtaagccgt ctgattcagc
     3841 tataatcttt ttgtttttta taactaaaaa atataaaata aaacttatct atctaacatt
     3901 ttattaacac tcaaattaga ataacgaaat gaaaaaatta acccctgatg aagccattga
     3961 tattgcctac gatattttct tagaaatggc aggcgaaaat ttagatcctg ccgatatttt
     4021 actgttcaac ctccaatttg aagagcgtgg tgcagtggaa atggtcgaaa cttctgaaga
     4081 ctgggatcaa gaaatcggga ctttaattga ccctgatgcc tttgccgaag tttgggttgg
     4141 cttagtcaat gaacaagatg aaatggacga tgtattcgcc cgtttcttaa tttcccacga
     4201 tgaagaaaac cgtgaatatc acgttgtttg gaaagaaaaa taaaaaggag aaaaaatggc
     4261 taatatcgac actaatcaac caatagaagc ctcgcttaaa tttcgtgaag caatggctca
     4321 cttatcttct gcggtgagca ttgtgacaac caatggcgta gccggcaaag cagggctgac
     4381 ggtttcgtct gtttgttccg ttaccgattc cccaccaacg ctcttatttt gcattaataa
     4441 aaacagccac gttcacgata ttatccaaca aaacggcaaa gtatgcgtta atgtgttaag
     4501 ccacgaacaa gaagacttag caaaacattt tgccgccatt ttagaaagta caatggaaga
     4561 acgctttggt tgggatattt ggaacaacgg ctttgacgat cagcctgtgt tacgcagtgc
     4621 tatttctgct ttacaaggca atattgttga tacgcacgca gtgggtacgc atactatttt
     4681 tgtggtagaa gtgagccata ttgacgtaga gccgaaccac agtttagttt attttgcccg
     4741 ccaatttaga agcgtgaaca ttgactaaaa tatatttaac agaaatttga tcaaatccga
     4801 cttttttagt cggatttttt atttcctgtt tttaaaacag tgctaaaatt ttgccgttta
     4861 tgtttgactg acaaaaggaa acaaaatgga aaatttaatt tgctacaaac aaatgccggt
     4921 gtggacaaaa gcaaccttac cacaaatgtt ccaagaaaag cacaacacca aagtcggaac
     4981 ttgggcaaaa ctcaccattc tcaaaggttc tctcaaatat tatgaattaa ccgaagaggg
     5041 agaaattatc aaggaagata tttttgatgc aaaccaccaa cgcccaatgg ttgagcctca
     5101 actttggcat aaggttgaag ctctttctga cgatttagaa tgccagttgg ctttctattg
     5161 caagccggaa gattattttg ctaaaaaata taatctgacc gctacccatt cagaagtgct
     5221 aaatgccgtc aattatacca aaggtggcaa agcgttagat ttaggttgcg ggcgaggacg
     5281 caatgcactt tatctgaatt tactgggttt tgatgtcact gctgttgatc ataatcaaga
     5341 aagcattgat tttctcaact atatgatcga aaaagaacaa gctcaaaaca ttactaccgc
     5401 tgtttatgat attaacgagg caactatcgg ctcgcaaaac ggtgaatatg acctgattat
     5461 ttccactgtt gtaatgatgt tcttaaaccg tgaacgcatt cctgccatta ttgagaatat
     5521 gcagaaaaac actaaagtcg gcggttataa cttaatcgta tgtgcaatga gtaccgatga
     5581 ctacccttgc ccgatgccgt tctcctttac cttcaaagaa ggcgagctcg ccgattatta
     5641 tcaaggttgg gaattagtca aatataacga agatttagga catttacaca aaactgatga
     5701 aaacggcaac cgcattcaac tacgttttgc gacaatgttg gcgaaaaaaa tcagctaaac
     5761 ttaaccgctt gcagggggaa ttagcttccc cctaattcat caaatccatt ctatttattt
     5821 tcactttttc attccgcaaa cgtttgcttg tgctataatg gcttcgtttt ttagtcattt
     5881 actgttgagg ttaaagtgag caacacacaa ctaagttaca aagatgccgg cgttgatatt
     5941 cacgcaggaa acgagttagt cgaacgaatc aagcctgatg ttaaacgcac ccgtcgccct
     6001 gaagtaatgg gcggattagg tggttttggt gctttatgtg ctttaccgac aaaatataaa
     6061 gagccgattt tagtttccgg taccgatggc gtcggcacaa aattacgctt agccattgac
     6121 ttaaacaaac acgatactat cgggcaagat ttggtagcga tgtgtgtgaa tgaccttgtc
     6181 gtgcaaggtg ctgaaccgct tttcttctta gattattacg ccaccggtaa attagaggtt
     6241 gatgtagcca ccaatgtcgt caaaggcatt gcagacggtt gcgtgatttc aggttgtgct
     6301 ttagtgggag gtgaaacggc tgaaatgccg ggaatgtacc acgaaggcga ttacgattta
     6361 gccggattct gtgtcggtgt ggtagaaaaa tctaaaatca ttgacggttc aacggtgaaa
     6421 accggtgatg ccttaattgc attagcgtcc agtggcccgc attccaacgg ctattcgtta
     6481 attcgtaaag tattggaagt cagccgatcc aatcctgcaa ccgatttatt ggaagacaaa
     6541 cccttaagcg aacacttatt agccccaact aaaatttatg taaaatcaat cctaaaatta
     6601 gttgaagaag ttgatgttca cgctatcgct cacttaaccg gtggcggttt ctgggaaaat
     6661 attccacgcg tattgccgga atctgccaaa gcggtgattg atgaatcgag ctggcaatgg
     6721 ccggcgatat ttagctggtt acaggaaaaa ggcaatatca gccgttatga aatgtaccgt
     6781 actttcaact gcggtgtcgg aatggtgata gccttacctg agagagatgt cgaaaaagcc
     6841 cttactcttc ttaaagagag tggtgaaaat gcgtggctta tcggtaaaat tgaagactta
     6901 ggcaccggta gcgagcaagt tgaaatccgc taatttgcaa aataaatgct aaaattgacc
     6961 gcttgtagta actgatctgc aaacggttaa atttttattg aggaatgcaa atgcaactat
     7021 tttggcctat ctactttgtc ttaatgaacc tagctgcctt atatttaatg tatagtgaca
     7081 aacaaaaagc gatcaaaaaa gaatggcgag tgccagaaat tactctcctc tctttttgcc
     7141 tcttaggtgg ggctatcggt gtttacctag gtatgaaata ctttcgccat aaaacccaac
     7201 attggtattt ccacgtcgct gttgtgcttt ctacttttgc ttggctgatt ggcttgcctt
     7261 acttttattt agctttatca tcatattaat ttatcattac tttttttaat ccgaaagccc
     7321 aatttatatc ctagtccgct cacaaaaaat ttgatttaga tcgcattatt tgtctattaa
     7381 aatcgttatt atctaccaaa atcgattgtc atttatttta aggagatgaa tatgtcagat
     7441 gtgcgtattg aacacgattt acttggtgaa agagaagtac ctaatagtgc ttattggggc
     7501 attcatacat taagagcgat tgagaatttt aatatctcaa atgtcaccat ttctgatgta
     7561 cctgaatttg tgcgtggcat ggtaatggta aaaaaagcta ctgcgttggc aaatggtgaa
     7621 ttgggtgcaa tcccgaaaaa aattgccaaa gctattgtgg cagcctgtga tgaagtatta
     7681 gtgaaaggac gctgtctcga tcaattccct tcagatgttt atcaaggtgg agccggcaca
     7741 tcggtgaata tgaataccaa tgaagtgatt gccaaccttg cattagaaat tttgggcgag
     7801 gcaaaaggta aatatgatat tgtcaatcca atggatcacg ttaatgcaag ccaatccacc
     7861 aatgatgcct atccaaccgg tttccgcatc gcggtttata acagtatcac taaattaatc
     7921 aataaaatca cttacctaca aactgcattt gaaaataaag ccactgagtt ttccgatgta
     7981 ctaaaaatgg gaagaacgca gttacaagat gccgtgccaa tgacgctcgg tcaggaattt
     8041 aaagcttttg ccgtattatt agatgaagag gttcgtaacc ttaaacggac ggcagaattg
     8101 cttcttgaag tgaatttagg cgcaaccgct atcggtactg gtctgaatac gccagagggt
     8161 tactctactc ttgcagttaa atatttagcc gaagtcacgc aactgccttg ctcactgtct
     8221 gaaaatctca ttgaggcaac atctgactgt ggtgcttatg taatggttca cggtgcatta
     8281 aaacgcaccg ccgtcaaact ttctaaagtg tgtaatgact tacgattact ttcatcaggc
     8341 ccacgtgccg gcttaaatga aattaatctt ccggaattac aagccggctc atctattatg
     8401 ccggcaaaag taaaccctgt tgtacctgaa gtcgtaaacc aagtgtgctt taaagtgatt
     8461 ggtaacgaca ccaccgttac ttttgcagca gaggccggtc aattacagct taacgtaatg
     8521 gagccggtta tcgggcaagc aatgtttgaa tctatcgagc tgctttccaa tgcatgtatt
     8581 aatctacgtg ataaatgtat tgacggtatt accgctaacc gtgacatctg ccaaaactat
     8641 gtgttcagct ctattggtat tgtgacctac ctcaatccat ttatcggcca ccataatggt
     8701 gatattgtcg gcaaaatttg tgcaaccacc ggcaaaagtg tacgagaagt tgtgcttgag
     8761 cgaggcttac tcaccgaaga acaactcgat gatattctct cgattgagaa cttaatgaat
     8821 ccgacttata aagccaaacg ctttaccgaa gaggaataat cattgtttac aagcggtctg
     8881 attctggcaa aaaattgcaa atttttacca aaatcagacc gcttgttatc ccattatttc
     8941 ttatttgctt tcagcatcgc cattaaattt gcaatatttg cagaagattt tgcctctttt
     9001 tcctgttcgg tcattcttgg cttttcactt tcccactcca aatcatcttg tggcaattcc
     9061 agtaaaaacc ggctcggctc aggtttgatg atttcgccat attggcgacg ttctttacac
     9121 agtgaaaacg tgagcgtttg ttgggctcgg gtaatgccga cataagccaa acgccgctct
     9181 tcttctacat tgtcctcatc aaggctggtt tggtgtggca gaatcccctc ttccataccg
     9241 attaagaaaa cgtgcggaaa ttccaaccct ttcgaggcgt gaagagtcat tagttgaact
     9301 tgatcggctt catcgtcctc ttcgccacgt tcgagcatat ctcgcaaggt taaacggttt
     9361 accacttgca ccaaattcat cggaggctct atttcatcgc cctctaacat cgcttgcacc
     9421 cattcaaaca aggtctgcac attacggctt tgcatttccg ctacttttgg gttcgccgcc
     9481 atttcataca aataggcttc atactgaatt ttcgccaaca gatcttttac tgcttcaatc
     9541 ggatcggatc tatcggcttg atcggcaatt tccacaatcc agcgggcaaa atcctgtaag
     9601 gcttggtaag gctttggcgt gaggcgttgt atgagttcaa aatcaaaaat cgcctcaaac
     9661 aaactgacct gcttttcatt agcaagtatg cctaattttt ctagcgttgc cgccccaatt
     9721 tcccgtttcg gcgtattcac aatacgcaag aaagctgcat catcgtcttg attcactacc
     9781 aaccgcagat acgccatcat atctttgatt tccgctcgag caaaaaatga cgtaccaccc
     9841 gaaattttat acggaatgcg gttttgcatt agcaattttt ccaacaagcg ggattgatgg
     9901 ttgcctcgat acaaaattgc ataatctttg tatttggtct ttttcgagaa acgatgggca
     9961 atcagctcgc ccacaatgcg ttccgcttcg tgttcctcat tttttgcctc aatgacctgt
    10021 aatttttccc cttgccccag ttctgaaaac agccgcttgc taaacaagtg atcgttatta
    10081 tcaatcagaa tattggcaca atgcagaatc cgctggctgg aacggtaatt ttgctcaagt
    10141 ttaatcactt gcaaatcaaa atcttcccgt aaccgttgca tattttcagg cttcgcccca
    10201 cgccaagagt aaattgattg gtcatcatcg cccacgaccg taaagtttgg ctcgttgccg
    10261 acaatcagct taattaactc atattggctg gtgttggtgt cttgatattc atcaactaac
    10321 aaatagcgaa ttttctgttg ccaacgagat tttgcttcgg gaaattggcg gaacagcaac
    10381 accggcaaca taatcaaatc atcaaaatcc aacgcattat aagccttaag ttgattctgg
    10441 tagagctgat aaaaatgtga aaacactcgc tcacgttcat cacgcacacg agtaagtacc
    10501 ctttcaggcg aaagcaaatc gtttttccag ttagaaatag tggcaattaa ggctttcagc
    10561 agatccttat cttccgtcac attttcaggc aataaatgtt tcagcaaagc aatttgatcc
    10621 tgctcatcaa acaaggtcat acccgattta aaaccgagca atttatattc gcgttttaaa
    10681 atttcaaacc caagtgtgtg aaaagtcgaa atggttaaac ctttgctgtt ttctttgccg
    10741 atagaatgat ccacccgttc acgcatttcc cgagcagctt tgttggtaaa ggttactgct
    10801 gcaatctgct taggcgaata gccacaatgt gcaattaaat gtgcgatttt attaataatc
    10861 acccgagtct tacccgagcc ggcaccagcc agcacaaggc aagccccttt ggtatattct
    10921 actgcttgtt gttgttgaga attgagtttc attgaatcta ttcaaaagta attttgacaa
    10981 ctattttaac ggaaagaaaa caagcggtcg aattttcagg aaaatttgca aaaaactttg
    11041 aaaatccgac cgcttgttca tcagagaaaa atccccaaaa cgcaaatttt tcctttcaat
    11101 cttgcccctt tagctatatc attataagtt gattttttag gggcaaatgc ctgattttat
    11161 aactcctttt ctttttaatt taagggaaaa aaacagtaga tggaaaaagt agaacagaaa
    11221 gctatcattg agcttaaaaa tgtaacaaaa agctacggta gtaaaacgat tcttaaaaat
    11281 ttaaatttaa ccattaacga tggtgaattt ttaacaattt taggcccttc cggttgtggt
    11341 aaaacaactg cgttacgttt aattgccggt tttgaagatt taactgaagg ttcaattatt
    11401 cttgatgggc aagatgtttc taacgttccg gcggagcaac gcccggtaaa caccgtattc
    11461 caaagctatg cccttttccc acatatgacg atttttgaaa acgtggcttt cgggctgcgt
    11521 atgcaaaaag tgccaaatgc tgaaattgag ccacgtgtga tggaagcatt acgaatggtg
    11581 cgtttagaag accgcgctca acaaaaaccg gctcagcttt ccggtggtca gcaacaacgt
    11641 attgcaattg cccgtgcggt ggtaaataaa ccgaaagtat tgttgttaga cgaatcgctt
    11701 tctgcgttag attataaatt gcgtaaagag atgcaaaatg aattaaaagc attgcaacgt
    11761 caattaggca ttacctttat ttttgtgacc cacgatcagg aagaagcctt aaccatgtct
    11821 gatcgtatta tcgtaatgaa cgctggtaaa atcgctcaag acggtacacc acgtgagatt
    11881 tacgaagagc caagtaactt atttgtagct cgtttcatcg gcgaaattaa cgtttttgat
    11941 gcaactgtaa ttgagcgtgt tgatgcaact aatgtaaaag caaacgtaga aggtcgcatt
    12001 tgcaatatca aagtggaaga tttagaggtt cagccgaacc aaaaattaaa agtattgtta
    12061 cgccctgaag atattgtgat tgaggagtta gacgaaaacg aaagctcgaa agcaattatc
    12121 ggtcatatta tcgaccgtaa ctacaaaggt atgacattag agtctagcgt gagattagat
    12181 cataacggta tgaatgtatt ggttagcgag ttcttcaatg aagacgatcc gaacattgac
    12241 cacgaagtgg gacaaaaagt tgctcttact tggtatgaag gctgggaggt tgtgttaaac
    12301 gatgaaggcg aataacaaat tccaaaatgg tgcggtcgca actattttcg gttggttatt
    12361 acttttcgta ttagtgccga acgtactcgt tttaatcatc agcttcttaa cagaaaaccg
    12421 tcaaagtcag tattttgtgg actttgcatt tagttttgat gcttataaat cactgtttaa
    12481 tgacacctat gcagcagtat tgtggaactc gctatatatg gcgggcattg ccaccttttt
    12541 ctgtttgatc atcggctatc cctttgcatt ttttattgcc aaaatgccgg aaagaattcg
    12601 tccgttttta ctgtttttag tagttctacc gttttggact aactcactga ttcgtattta
    12661 tggcattaag attttcttag gcgtaaaagg ctttttaaat gaaagcttga tggctttagg
    12721 tataatcagc gagccaatgc gtttattaaa ctcagaaatt gcgattatta ttggtttagt
    12781 ttacattcta ttacctttta tgattctacc gctttattca tcaatcgaga aaattgataa
    12841 ccgcttacta gaagctgcaa aagacttagg tgcaaacgcc ttccagcgtt ttattaaagt
    12901 gactattcca ttaacaatgc cgggcattgt cgcaggttgt ttattagtat tacttccggc
    12961 aatgggtatg ttctatgtgg cagatttatt aggcggcggt aaaacaccgt tagtaggtaa
    13021 cattattaag agcttgttct taaataccaa ccaatttgca ttaggttcag cagtaagtat
    13081 tgcattaact atcttaatgg cattaatgct ttatgtttac tatcgtgcaa acaaattatt
    13141 aaataaaaag gtggagcttg aataatgaaa aagatgttta gaaacctttt tatgttggtt
    13201 gtttttgcat atctctatat tccaattatc attttagtga tcaactcctt taatgaagac
    13261 cgctttggat taaattggaa aggctttact tggaattggt atgagcgttt attcagtaat
    13321 gatacgcttg tacaagcaac attgaactca ttaactattg cgttctttgc ggcaacattc
    13381 tcaaccattt taggggcatt aacctctatt gcgctttacc gttaccgttt ccgtggtaaa
    13441 aagctagtgg gcggaatgtt attcgtggta atgatgtcgc ctgatatcgt aatggcggta
    13501 tctatgctcg tattatttat gatcttaggt atcaagctag gctttatttc gctattaatt
    13561 gcacacatta ctttctgttt gccttatgtg gtaattgcaa tttcctcacg cctcagcgat
    13621 tttgacggca aaatgctaga agctgcaaaa gacctaggtg caagtgaagt gactattttg
    13681 cgtaaaatta ttttaccact ggcattacct tcgattattt caggctggtt gttaagcttt
    13741 acgatttcgt tagatgacgt tattgtttca tctttcgtga caagcccaag ctacgaagtt
    13801 ttaccactga aaattttctc attggttaaa acaggtgtaa cacctgaagt gaatgcacta
    13861 gcaactatta tgattatttt ctcat
//
LOCUS       DANLUQ010000054        13519 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.054, whole genome shotgun sequence.
ACCESSION   DANLUQ010000054 DANLUQ010000000
VERSION     DANLUQ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13519
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.054"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..672)
                     /gene="mtnN"
                     /locus_tag="PXB27_001257"
     CDS             complement(<1..672)
                     /gene="mtnN"
                     /locus_tag="PXB27_001257"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010786393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDL5346051.1"
                     /translation="MKIGIIGAMAQEIEILRQVMVEPKVTELAGCKIFEGKINNTRVA
                     LLQSGIGKVAAAIGTTLLIQLTQPDMIINTGSAGGLDPDLNVGDILISTEVRYHDVDV
                     TAFGYEIGQLPANPPTFSPNEQLVELAQKEAKKAGYNVVSGLICSGDAFINGNDKINQ
                     IRQNFPNVVAVEMEAAAIAQVCHGFNLPFVIVRAVSDVADKESHLSFEEFLPLAAEKS
                     STIVLA"
     gene            complement(730..951)
                     /locus_tag="PXB27_001258"
     CDS             complement(730..951)
                     /locus_tag="PXB27_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HDL5346052.1"
                     /translation="MDNNYTLSAKITELETKVAFQELIIEELNQALIEQQFAIDKLQT
                     QIRHIAEKLKGAQLSQVASQAEETPPPHY"
     gene            1039..1764
                     /gene="fkpA"
                     /locus_tag="PXB27_001259"
     CDS             1039..1764
                     /gene="fkpA"
                     /locus_tag="PXB27_001259"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL5346053.1"
                     /translation="MLKTKLSLFTLIVGTLFSTNVMAEEKADPKFVDDSSYAVGVLMG
                     KNINGVIESQKDIFTYNQDKILAGVQDTLKKTGKLTDEDLQKQLKSLDAYLSAKEAKI
                     NEEKTKATVAAGDKFRADYKKQSGVKETASGLLYKIEKAGEGNSPKAEDTVKVHYKGT
                     LTDGTVFDSSYDRGEPIEFQLNQLIPGWIEAIPMLKKGGKMEIVVPPALAYGDRAAGK
                     IPANSTLKFEIELLDFKAADAKK"
     gene            1861..2517
                     /locus_tag="PXB27_001260"
     CDS             1861..2517
                     /locus_tag="PXB27_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL5346054.1"
                     /translation="MTKKFTPFSDEDHAILASYFPMVDGIAALIGEHCEIVLHSLEFL
                     EHSAIYIANGHNTNRKIGSPITDRALKSLHHMATDSVSKPYFTKAKGGVLMKSITIAI
                     RNGKQHVIGLLCININLEVPTSQFLNSFIAPVDTASEVTFASSVEDLVSQTIQTTIDD
                     VMADRQISNNNKNRQIVISLFEKGIFDIKDSINQVAEKLDISRHTVYLYIRQIKQDND
                     "
     gene            2517..2894
                     /gene="tusD"
                     /locus_tag="PXB27_001261"
     CDS             2517..2894
                     /gene="tusD"
                     /locus_tag="PXB27_001261"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HDL5346055.1"
                     /translation="MDYVLAIKSPVYGSQGAFLAYQVAEELLKANHTICQIFFFQEGV
                     SNANALVNPASDEVNLVEMWKKLAKLHRLPLHLCIAAAQRRGVVDEQISANATHNLAE
                     GFTLAGLGEFSQAVLKADRLLTF"
     gene            2896..3267
                     /gene="tusC"
                     /locus_tag="PXB27_001262"
     CDS             2896..3267
                     /gene="tusC"
                     /locus_tag="PXB27_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HDL5346056.1"
                     /translation="MKKYKLAFLFTQPPFGSAVSREGLDALLAASAFCDEQEIAILFI
                     NDGVFNLIADQQPEKLLQKDHISTFKLLELYDLTECFICQESVSLRGLDNADWLLRML
                     QFKTHAEMIALLNQAEKVMTF"
     gene            3277..3552
                     /gene="tusB"
                     /locus_tag="PXB27_001263"
     CDS             3277..3552
                     /gene="tusB"
                     /locus_tag="PXB27_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HDL5346057.1"
                     /translation="MLYTLSKANYDEETLNFFFSHLSENDAVVLWQDGVLQAVKSPQF
                     FAKLPRCYLLAQDVQARGLQEKLTAFQCISLSELVKLTAVYFPQVAL"
     gene            3668..4566
                     /locus_tag="PXB27_001264"
                     /pseudo
     CDS             3668..4566
                     /locus_tag="PXB27_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484023.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4916..8941
                     /gene="rpoB"
                     /locus_tag="PXB27_001265"
     CDS             4916..8941
                     /gene="rpoB"
                     /locus_tag="PXB27_001265"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDL5346058.1"
                     /translation="MAYSYSEKKRIRKSFGKRPQVLNVPYLLTIQLDSFEKFIEKDPE
                     GQQGLEAAFRSVFPIVSNNGATELQYVSYELGEPVFDVRECQIRGTTYAAPLRVKLRL
                     VSYDREAAAGTVKDIKEQNVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFARIDRRRKLPATIILRALGYTTE
                     EILNLFFEKIKFEIADNKLLMTLVPERLRGETASFDIAANGKVYVEMGRRITARHIRA
                     LEKDNVTQIEVPVEYIVGKVAAQDYVDLSSGEVICPANMEISMEMLANLSQAGYNQIE
                     VLFTNDLDHGPYISETLRVDPTYDRLSALVEIYRMMRPGEPPTKEAAEALFDNMFFSA
                     ERYDLSAVGRMKFNRSLEIPEGVGSGILSNDDIIGVMKKLIEIRNGRGEVDDIDHLGN
                     RRIRSVGEMAENQFRIGLVRVERAVRERLSLGDLDGVTPQDLINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEVTHKRRISALGPGGLTRERAGFEVRDVHPTHYGRLCPIETPEG
                     PNIGLINSLSVYARTNNYGFLETPFRKVVNGQVTEEIEYLSAIEESNNIIAQANSNLD
                     ENFRFTDTYVTCRQQGESGLFKPEEVQYMDISTQQVVSVAAALIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMEKPIALDSGVAIVAKRGGTVQYVDASRIVVKVNEDE
                     TIPGEAGIDIYNLIKYTRSNQNTCINQVPCVNLGEPVARGEILADGPSTDLGELALGQ
                     NIRVAFMPWNGYNFEDSMLVSERVVQEDRFTTIHIQELSCVARDTKLGAEEITADIPN
                     VGESALSKLDESGIVYIGAEVKGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGTSGTVIDVQVFTRDGVEKDKRAKEIEEMQLCEAKKDLAEELEILEAGLF
                     TRVRNLLIEGGVKESALDGVAREKWLEQTLEDEAKQNQLEQLAEQHEELRKEFERKLE
                     IKRNKIIQGDDLAPGVLKVVKVYLAVKRQIQPGDKMAGRHGNKGVISKINPVEDMPYD
                     ENGQPVEIVLNPLGVPSRMNIGQILETHLGLAAHGIGDQIDKMIKQQQEVAKLREYMQ
                     KAYDLGHGSQVVDLSTFSDEEVMRLAQNLRKGLPLATPVFDGAHESEIKGLLELGGLP
                     TSGQITLYDGRTGEKFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGKA
                     QFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGTHYMEPGIPE
                     SFNVITKEIRALAIDMELDEA"
     gene            9204..13487
                     /gene="rpoC"
                     /locus_tag="PXB27_001266"
     CDS             9204..13487
                     /gene="rpoC"
                     /locus_tag="PXB27_001266"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDL5346059.1"
                     /translation="MKDLVKFLKAQTKSNEDFDVIKIGLASPDKIRSWSFGEVKKPET
                     INYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRER
                     MGHIELACPVAHIWFLKSLPSRIGLILDMPLRDIERVLYFESYVVTEPGMTDLEKNQL
                     LTEEQFMDAEERWGDEFEAKMGAEGIQALLRDLDLEHQCETLREELQETNSETKRKKI
                     TKRLKTLEAFLQSGNKPEWMVMTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINR
                     NNRLKRLLDLVAPDIIVRNEKRMLQESVDALLDNGRRGRAITGSNKRPLKSLADMIKG
                     KQGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFRPFIYSKLENRGIA
                     STIKAAKKMVEREDPIVWDILAEVIREHPILLNRAPTLHRLGIQAFEPLLIEGKAIQL
                     HPLVCAAFNADFDGDQMAVHVPLTLEAQLEARALMMSTNNVLSPASGDPIIVPSQDVV
                     LGLYYMTREKVNGKGEGMYFLDPREAEKAYRTGQAELHSRVKVRVTEYEKNEAGELEA
                     KTSLVDTTIGRSILWMIAPKGMPFSLFNQTLGKKAISKLINESYRRLGLKESVVLADQ
                     IMYTGFAYAARSGASVGIDDMVIPEQKYAIIEAAEAEVAEIQEQFNGGLVTAGERYNK
                     VIDIWAAANERVAKAMMENLSTEEVINREGNPEKQASFNSIFMMADSGARGSAAQIRQ
                     LAGMRGLMARPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLT
                     RRLVDVAQDLVIIEDDCGTHEGIVMTPLIEGGDVKEALRDRVLGRVVAEDVLKPGTDE
                     VLIPRNTLLDEKWCDVVDRESVDVIKVRSVVTCDTDFGVCAKCYGRDLARGHLINQGE
                     AVGVIAAQSIGEPGTQLTMRTFHIGGAASAAAKESSIQVKNAGTIKLTNAKFVTNKEG
                     KIVLTSRNTELTVIDTFGRTKENYKVPYGAVLSKHDGAEVAVGEVVANWDPHTMPVIS
                     EVSGRIQFSDIVDGLTVTRQTDELTGLSSIVVQDVGERATAGKDLRPALRLVDSNGKD
                     ILIPGTDVAAQYFLPGKAIVTLDDGAEIEVGEALARIPQESVGTKDITGGLPRVADLF
                     EARKPKEPAILAEISGIVSFGKETKGKRRLVITPAEGEAFEEMIPKWRQLNVFEGEMV
                     QRGDVISDGAETPHDILRLRGVHAVTDYIVNEVQEVYRLQGVKINDKHIEVIVRQMLR
                     KAVITNAYDSEFLEGEQVEVARVKIANRKRAEEGKPLVEFERELLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKQDELRGLKENVIVGRLIPAGTGFAYHQARAKKRAQLEA
                     GIAVVPEFEAPAAKANSFVTDADIEAEFEFVADDATQSLAALLNAGEEE"
BASE COUNT         4062 a         2623 c         3065 g         3769 t
ORIGIN      
        1 tgctaataca atcgtggaag atttttctgc ggctaatggc agaaattctt caaaagaaag
       61 gtgggattct ttgtccgcta catccgatac tgctcttacg attacaaatg gaaggttaaa
      121 accgtgacaa acctgcgcaa tggctgctgc ttccatttcg actgcaacga cattcgggaa
      181 attctgacga atttgattaa tcttatcatt accgttaata aaggcatcgc cgctacagat
      241 taagcctgaa accacattgt aaccggcttt tttcgcttcc ttttgagcta attcgactaa
      301 ttgctcgttt ggcgaaaatg ttggcgggtt tgccggtagt tgcccgattt cataaccaaa
      361 ggcggtcaca tcaacatcgt gataacgaac ttcggttgaa atcaaaatat cacctacgtt
      421 taaatctgga tctaatccac cggcagagcc tgtattaata atcatatccg gctgggtgag
      481 ctgtatcagt aaggtagtgc cgatggcggc agcaactttg ccgatacccg attgtaataa
      541 tgccacacgg gtattgttaa ttttgccttc aaaaatttta caacctgcta attcagtgac
      601 tttaggttca accattactt ggcgtaaaat ctcaatttct tgtgccatcg caccaataat
      661 accaattttc attactttcc ttctctaatg tattgtttat tttttgaaaa aaacgctatt
      721 ttaacagcat taatagtgag gagggggtgt ttcttctgct tgtgaagcaa cttggcttag
      781 ttgagcccct ttcaatttct cagcaatatg gcgaatctga gtttgtaact tatcaattgc
      841 aaattgttgc tcaataagtg cttgatttaa ctcttcaata atcagctctt ggaaagcaac
      901 tttagtctcc aattcggtaa tttttgccga taatgtataa ttattatcca ttttttacta
      961 aaaaaaggtt gtgtgaaaag gaattacact ttaccctatc ttaaattttc ttaacagtta
     1021 tttttataag gaaaaattat gttaaaaacc aaattatcat tatttactct tattgttggc
     1081 acacttttta gtacaaatgt gatggcagaa gaaaaagcag atcctaaatt tgtggatgat
     1141 tcctcttatg ccgtgggtgt attaatgggt aaaaatatta atggtgtgat tgagtcacaa
     1201 aaggatattt ttacctataa ccaggataaa atcttagccg gtgttcagga caccttaaag
     1261 aaaaccggta aattaaccga tgaagatttg caaaagcagt taaaatcgtt agatgcctat
     1321 ttatctgcga aagaagcaaa gattaacgaa gaaaaaacca aagcaacggt agctgcaggc
     1381 gataaattcc gtgctgatta taaaaaacag tctggcgtaa aagaaacggc ttcaggcctg
     1441 ttatataaaa ttgaaaaagc aggtgaaggc aattcaccaa aagcggaaga tactgtgaag
     1501 gtgcattaca aaggcacttt gactgatggc acggtatttg acagctctta cgaccgtggt
     1561 gagccgattg aatttcagtt aaatcaatta attccgggtt ggattgaagc gattcctatg
     1621 ttgaaaaaag gcggtaagat ggaaattgta gtgccacctg cattagctta tggtgatcgt
     1681 gccgcaggca aaattccagc aaattcaacc ttgaaatttg aaattgaatt attagatttt
     1741 aaagcagcag atgctaagaa ataattcgtt attccttaat tattgaagat acgtaagcgg
     1801 ttagatttat tataatattt gcaaaaaatg tgataaaact aaccgcttgt aatttgtatt
     1861 atgaccaaaa aatttacgcc tttttctgac gaagatcacg ccattcttgc ttcttacttt
     1921 ccgatggtag atgggattgc ggcactgatt ggtgagcatt gcgaaattgt attgcattcg
     1981 ttagagtttc tggaacattc ggcaatttat attgctaacg gacacaatac taaccgtaaa
     2041 atcggctctc cgattactga ccgtgcattg aaatcgctgc atcatatggc aacggatagc
     2101 gtctccaaac cttattttac caaggcaaaa ggtggcgttt taatgaagtc tatcactatt
     2161 gcgattcgta atggtaaaca gcacgttatc gggttactgt gcattaatat taatttggaa
     2221 gtgcctacct cacagtttct caattctttt attgcccctg tagataccgc ttcagaagtt
     2281 acttttgcca gttcagtaga agatttggtt tcacaaacga ttcaaacgac cattgatgat
     2341 gtgatggcag atcgtcaaat ttctaacaat aataaaaatc gtcaaattgt gatttcgtta
     2401 tttgagaaag ggatttttga tattaaagat tccattaacc aagtagcgga gaagctggat
     2461 atttcacgcc atactgtgta tctctatatt cgtcaaatta agcaggacaa tgattaatgg
     2521 attatgtatt agcgataaaa tcaccggttt atggttcaca aggggcattt ttagcctacc
     2581 aagtggcgga agaattatta aaagccaatc atacaatttg ccaaatattc ttttttcaag
     2641 aaggcgtgag caatgctaat gccttagtga atccagcaag cgatgaagtg aatttagtcg
     2701 agatgtggaa aaaattggct aaattgcacc gcttgcctct gcatttgtgc attgctgctg
     2761 cccagcgtag aggcgtggtg gatgagcaaa tctcagctaa tgcaacacat aatttagccg
     2821 aaggatttac cctagccggg ttaggcgaat ttagccaagc agtattaaaa gcggatcgat
     2881 tactgacttt ttaagatgaa gaaatataaa cttgcctttt tatttaccca gccacctttt
     2941 ggttcggcag ttagccgtga ggggctagat gccttgcttg cagctagtgc tttttgtgac
     3001 gagcaagaga tagctatcct ctttatcaat gatggcgtat ttaatctcat tgccgatcaa
     3061 cagccggaaa aactcttaca aaaagatcat atttccacct tcaaattatt ggagttgtat
     3121 gacttaactg aatgttttat ttgccaagaa agtgtaagtt tacgagggct ggataatgca
     3181 gattggttac tgcgaatgct tcaatttaaa actcacgctg agatgatcgc attgctcaat
     3241 caagctgaaa aagtaatgac gttttaagga atatttatgc tttataccct ttccaaagcg
     3301 aattatgatg aagaaacctt aaacttcttt ttctcacatc ttagtgagaa cgatgcggtt
     3361 gttctgtggc aagatggcgt gctacaagcg gtcaaaagtc cgcaattttt tgcaaaattg
     3421 ccccgttgct atttattggc acaagatgtg caagctcgag gattgcaaga gaagctcact
     3481 gcatttcagt gcatttcatt atccgaatta gtcaaattaa cagcggttta ttttccccag
     3541 gttgcactgt aacctagaga ggatattttt tgtaatacca aataatttag attatgatgt
     3601 gattcctaaa aaggagaata ttatgaaaac aaaatgtctt attccgctac tagcacttac
     3661 acctgttatg gtgatggcag aagaaaataa cacttgggta gacaagaaac atgaaacagt
     3721 aagcactacc ttagatcgtt gggcaaacaa tattaacagt tggctaggtg atgcagaccc
     3781 agataaaccg ggaacggctt ctttacgggt aatgttagat agccgttggg ataaacacga
     3841 taaatttact tataagcccc gtgttcgtgg taaaatcaaa ctccctatct tgaaaaagca
     3901 tctaaatgtc gtatttggtg atgacgaact ggataatgag cttaccgata aaaatcatgc
     3961 tggaaaattg tataaaaacg tcaatccaaa taaacgctat aactctcgtg aagcgagaga
     4021 ttcgaactct tctatcggtt tacgttggtc tgatggcatt aaggctttag ggattgagac
     4081 cgatttagat gctggtattc gttcgggaag cgatattttc gggcgtttac gcatcagtaa
     4141 agattggaaa atcaccgacc agttcagtac ccgcctagag caaatttatc gttacggttc
     4201 agagagcaag cactatttac gtactaactt tgaaaataaa tataccgaaa cggataccac
     4261 ctttattatg aaccacacca attttgaata tcgtcatgat gtagatgaag aaagaagttg
     4321 ggggaatagt ttatatcgtc aacataactt caaaccactc acgcacatca gctatggtat
     4381 cagtatgggg ggcgtttaga taaagagtta tcaaaattta actattgggg tccatttgtc
     4441 tcctatcgtc aaccaatctg gcgcaactgg ctctttatcc agcctgaaat cagtttttat
     4501 aacgacaaag ataaagatcg taagcacttt attcagacat ttgtacgttt agaggctatc
     4561 ttctagctat taagcctcaa gtagaatctt ggggcttgtt tttgcaagcg gtcaatttct
     4621 tgccaaaatt tacaaaatcc gctataattc ctaaccattt ttagcaaaaa ttggctgacg
     4681 gcttgaccgg tcagcctttt gtgcttgaac gcaagggctg attttgtcat tttaatcagc
     4741 aaaatttgca aatttcctgc ccaatttgac cgcttgcagt agcgatttct gactgcttga
     4801 ttggcgattg gaaaatgaca tctcgtacag tttttataat gccacttcca accgagtctt
     4861 tcgtgtcgga ttgtgtaatc tcaaccaaaa tatatttcag aggctatctt aaaaaatggc
     4921 atattcatat tccgagaaaa agcgtatccg taagagcttt ggcaaacgcc cacaagttct
     4981 gaacgtacct tatctattaa caattcagct tgattctttt gaaaaattta tcgaaaaaga
     5041 tcctgaaggg cagcaaggtt tagaagcggc attccgttct gttttcccga ttgtgagcaa
     5101 caatggtgca acagaattac aatacgtttc ttacgaatta ggcgagccgg tgtttgacgt
     5161 gcgtgagtgc caaattcgtg gtacaaccta cgctgcacca ttacgcgtga aattgcgttt
     5221 agtctcttac gatcgtgaag ccgcagccgg tacggtaaaa gacattaaag agcaaaacgt
     5281 gtatatgggc gaaatcccat taatgaccga taacggtact ttcgtgatta acggcaccga
     5341 gcgtgttatc gtttctcaat tacaccgtag cccaggtgtg ttcttcgatt ctgacaaagg
     5401 taaaacccac tcttcgggta aagtgcttta taacgcacgc attatccctt accgtggttc
     5461 ttggttagat ttcgagtttg atccgaaaga taacttattt gcccgtatcg accgccgtcg
     5521 taaattgccg gcaaccatta ttctacgtgc attaggctac accaccgaag aaatcttaaa
     5581 tttattcttc gagaaaatta aatttgaaat tgccgataat aaattattga tgacgttagt
     5641 gcctgagcgt ttaagaggcg agactgcatc atttgatatt gcagcaaacg gcaaagtata
     5701 tgttgaaatg ggtcgccgta ttaccgctcg tcatatccgt gcgttagaaa aagacaatgt
     5761 gacccaaatt gaagtgccgg tggaatatat tgtcggcaaa gtagcggctc aagattacgt
     5821 tgatttatct tcaggtgaag tgatttgccc ggcgaatatg gaaatctcga tggagatgtt
     5881 ggcgaattta tcacaagccg gttacaacca gatcgaagta ttatttacca acgatttaga
     5941 ccacggtcca tatatttctg aaaccttacg tgttgatcca acttacgacc gtttaagtgc
     6001 gttggttgaa atctatcgta tgatgcgtcc gggcgaacca ccaacgaaag aagcggcaga
     6061 agcattattc gataatatgt tcttctcggc agagcgttat gatttatcgg cagtaggtcg
     6121 tatgaaattc aatcgctcgt tagaaattcc ggaaggcgta ggcagcggta ttttaagcaa
     6181 cgatgacatc atcggtgtga tgaaaaaatt aatcgaaatc cgtaacggtc gtggtgaagt
     6241 ggatgatatc gaccacttag gtaaccgtcg tatccgcagc gttggtgaaa tggcagaaaa
     6301 ccaattccgc attggtttgg tgcgtgtaga acgtgcggtg cgtgagcgtt tatctttagg
     6361 cgatttagat ggtgtaacac cacaagattt aatcaacgct aaaccgattt cagcagcagt
     6421 gaaagagttc tttggttcat cacaactttc gcaatttatg gaccaaaaca acccgctttc
     6481 agaggtaaca cataaacgcc gtatctcggc attaggcccg ggtggtttaa cccgtgagcg
     6541 tgccggcttt gaagtgcgtg acgtacaccc gactcactat ggtcgtttat gtccaatcga
     6601 aactcctgaa ggcccgaata tcggtttgat caactcacta tcggtgtatg ctcgtactaa
     6661 taactacggt ttcttagaga ctccattccg taaagtggtg aatggtcaag taaccgaaga
     6721 gatcgagtat ttatctgcga ttgaagaaag caacaatatc attgcacagg cgaactcaaa
     6781 cttagatgag aacttccgtt ttaccgatac ttatgtaact tgccgtcagc aaggcgaatc
     6841 gggcttattc aaaccggaag aagttcaata tatggacatc tcaacccaac aagtggtttc
     6901 tgtggctgcg gcgttaattc cgttccttga gcacgacgat gcgaaccgtg cgttaatggg
     6961 tgcgaacatg caacgtcagg cagttccaac cttgcgtgcg gacaaaccat tagtcggtac
     7021 cggtatggaa aaaccaattg ctcttgactc aggtgtagcg attgtcgcaa aacgtggtgg
     7081 tacggtgcag tatgttgatg cgtcacgcat tgtagtgaaa gtaaatgaag atgaaaccat
     7141 tccgggcgaa gcgggtatcg atatttataa cttaatcaaa tacacccgtt caaaccaaaa
     7201 cacctgtatc aaccaagtac cttgtgtgaa cttgggtgag ccggttgctc gtggtgaaat
     7261 cttagcggac ggtccttcaa cagacttagg tgaattggca ttaggtcaaa atatccgtgt
     7321 ggcatttatg ccttggaacg gttataactt cgaagactca atgttagttt ctgagcgtgt
     7381 ggtacaagaa gatcgcttta ccactatcca cattcaagaa ttatcttgtg tggcgcgtga
     7441 taccaaatta ggtgcagaag agatcaccgc tgatattcca aacgtgggtg aatctgcatt
     7501 aagcaaatta gatgaatcag gtatcgttta catcggtgca gaagtgaaag gtggcgacat
     7561 cttagtaggt aaagtaactc ctaaaggtga aacccaatta acaccggaag aaaaactatt
     7621 gcgtgcgatc ttcggtgaaa aagcctctga tgtgaaagat tcatcattgc gtgttccaaa
     7681 cggtacttca ggtacggtta tcgatgttca agtatttacc cgtgatggcg tagagaaaga
     7741 taaacgtgcg aaagaaatcg aagagatgca actgtgtgaa gcgaagaaag acttagcgga
     7801 agagttggaa atcttagaag ccggtttatt tactcgtgtt cgcaacctat taattgaagg
     7861 cggcgtaaaa gaatctgcat tagatggcgt tgctcgtgaa aaatggttag aacaaaccct
     7921 tgaagacgaa gcgaagcaaa atcagttaga acagttagcg gaacagcacg aagagttacg
     7981 caaagaattt gaacgtaaac ttgagatcaa acgcaacaaa attatccaag gcgacgattt
     8041 agcaccgggt gtgttaaaag tagtgaaagt ttaccttgcg gtgaaacgcc aaatccagcc
     8101 gggtgataag atggcgggtc gtcacggtaa caaaggggtt atctcgaaaa tcaaccctgt
     8161 ggaagatatg ccatacgatg aaaacggtca gccggttgaa atcgtattga acccgctggg
     8221 cgtaccgtct cgtatgaaca tcggtcagat cttagaaacc cacttaggct tagcggctca
     8281 tggtatcggt gatcagattg ataagatgat caaacagcaa caagaagtgg ctaaattacg
     8341 tgaatatatg caaaaagcct acgatttagg tcacggctca caagtcgttg atttaagcac
     8401 tttctctgat gaagaagtga tgcgtttagc gcaaaactta cgcaaaggct taccgcttgc
     8461 tactccggta ttcgatggtg cacacgaaag cgaaatcaaa ggcttattag agttaggcgg
     8521 cttaccgact tcaggtcaga tcacacttta tgatggtcgt accggtgaga aatttgagcg
     8581 tccggtaacc gttggttata tgtatatgct caaattgaac cacttggttg atgataaaat
     8641 gcacgctcgt tcaaccggtt cttatagcct tgttactcaa caaccattgg gtggtaaagc
     8701 tcaattcggt ggtcagcgtt tcggtgagat ggaggtttgg gcattagaag catacggtgc
     8761 ggcttatact cttcaagaaa tgcttacggt gaaatcggac gatgtgaacg gtcgtacgaa
     8821 gatgtataaa aacattgtag atggtacaca ttatatggag ccgggtatcc ctgaatcctt
     8881 taacgtaatt accaaagaga tccgtgcatt agcaattgat atggagctgg acgaagcgta
     8941 atggtttcac tctccccctt gatgggggag agacaggcaa aggatttgtt cagaatcctt
     9001 tggcagagag agggtgatgc aagcggtgta aatttgctaa atttttgcaa aaatcgaccg
     9061 cttaccctct ccctgatttt gcttctgaac gaggcaaaat ctatccctct cccacaaggg
     9121 gagagggttt aaaatcgcaa ccaaattcgt agagtggata ttctttccac caaacaaaac
     9181 aacctcaaac taggggcaaa aaagtgaaag acttagtgaa gtttttaaaa gcacaaacaa
     9241 aatcgaacga agattttgat gtgattaaaa ttggtttagc atcaccggac aagatccgtt
     9301 cttggtcttt tggtgaagtg aaaaaaccgg aaaccattaa ctatcgtacc ttcaaaccgg
     9361 agcgtgatgg tttattctgt gcacgtattt tcgggccggt aaaagattac gagtgcttgt
     9421 gtgggaaata caaacgttta aaacaccgtg gcgtaatttg tgaaaaatgt ggtgttgaag
     9481 taacccaaac taaagtacgt cgtgagcgta tggggcacat tgaattagct tgccctgtcg
     9541 cacacatctg gttcttaaaa tcactaccgt cccgtatcgg tttaatctta gatatgccgt
     9601 tgcgtgatat tgaacgtgtg ctttattttg aatcttatgt agttactgaa ccgggaatga
     9661 ccgatttaga gaaaaatcag ttattaaccg aagaacaatt tatggacgct gaagagcgtt
     9721 ggggtgatga atttgaagcg aaaatgggtg cagaaggtat ccaagcatta cttcgtgatt
     9781 tagatttaga acaccaatgc gaaaccttgc gtgaagagtt acaagaaacc aattcagaaa
     9841 ccaagcgtaa aaaaatcact aagcgtttaa aaaccttaga agcattctta caatccggca
     9901 acaaaccgga atggatggtg atgacagtat taccggtact tccaccggat ttacgtccgt
     9961 tagtaccgct tgacggtgga cgttttgcga cttctgactt aaacgattta taccgccgtg
    10021 taattaaccg taacaaccgt ttaaaacgct tgttagattt agttgcaccg gacatcatcg
    10081 tgcgtaacga aaaacgtatg ttacaagagt cggttgatgc gttattagac aacggtcgcc
    10141 gtggtcgtgc gattaccggt tcgaacaaac gtccgttaaa atctttggca gatatgatca
    10201 aaggtaaaca aggtcgtttc cgtcaaaact tattaggtaa acgtgtagat tactcaggtc
    10261 gttcggtaat taccgtaggc ccatacttac gcttacacca atgtggttta ccgaagaaaa
    10321 tggcgttgga attattccgt ccgtttatct actcaaaatt agaaaaccgt ggcattgctt
    10381 caactattaa agccgcgaag aaaatggtag agcgtgaaga tcctatcgta tgggatattc
    10441 ttgccgaagt gattcgtgaa cacccgattc tacttaaccg tgcaccaaca cttcaccgtt
    10501 taggtatcca agcgtttgag ccgttattga ttgaaggtaa agcgatccag ttacacccac
    10561 tcgtgtgtgc ggcgttcaat gcggacttcg acggtgacca aatggcggtt cacgttccat
    10621 taacccttga agcacagtta gaagctcgtg cgttaatgat gtcaaccaac aacgtacttt
    10681 caccggcaag tggtgatccg attatcgtac cgtctcagga cgttgtactc ggtctttact
    10741 atatgacccg tgagaaagta aacggtaaag gtgagggaat gtacttcctt gaccctcgtg
    10801 aagcggaaaa agcataccgt accggtcagg ctgaattaca ttctcgtgtt aaagtgcgtg
    10861 ttactgaata tgagaaaaac gaagcaggcg aattagaagc aaaaacttcg ttagtagata
    10921 ccaccattgg tcgttcaatc ttatggatga ttgcaccaaa aggtatgccg tttagcttgt
    10981 tcaaccaaac cttaggcaaa aaagcgatct cgaaattaat taacgagagc tatcgccgtt
    11041 taggtttaaa agaatctgtg gttttagctg accaaattat gtacaccggc tttgcttatg
    11101 ctgctcgttc tggtgcctca gtaggtatcg acgatatggt aattccggag caaaaatacg
    11161 caatcattga agcggctgaa gcggaagtcg ctgagatcca agagcaattc aacggcggtt
    11221 tagtaacagc cggtgagcgt tataacaaag tgatcgatat ttgggctgct gcaaacgaac
    11281 gtgttgcgaa agcaatgatg gaaaaccttt caacggaaga agtaatcaac cgtgaaggta
    11341 atccggaaaa acaagcctca tttaacagta tctttatgat ggcggactcc ggtgctcgtg
    11401 gttctgctgc acagattcgt cagttagcgg gtatgcgtgg cttgatggct cgtccggacg
    11461 gttcgattat cgaaacccca attactgcaa acttccgtga aggtttgaac gtattacagt
    11521 actttatttc aacccacggt gcacgtaaag gtcttgccga tacggcatta aaaacagcga
    11581 actccggtta cttaacccgt cgtttagttg atgtggcaca agatttagtt atcattgaag
    11641 atgactgtgg tacgcacgaa ggtatcgtga tgacaccact tatcgaaggt ggtgatgtga
    11701 aagaagccct gcgtgatcgt gtattaggtc gtgtggtggc agaagatgtg ttaaaaccgg
    11761 gtacagatga agtattaatt ccacgcaaca ccttattaga tgaaaaatgg tgtgatgtgg
    11821 tggatcgtga atctgtggat gtgatcaaag tacgttcggt agtaacttgt gatacagact
    11881 tcggtgtgtg tgctaaatgt tacggacgtg acttggctcg tggtcactta atcaaccaag
    11941 gtgaagcggt cggtgttatt gcggcacaat ctatcggtga gccgggtaca cagttaacga
    12001 tgcgtacgtt ccatatcggt ggtgcggctt ctgcggcagc aaaagaatct agcatccaag
    12061 tgaaaaacgc aggtacgatt aaattaacca acgctaaatt tgtaaccaac aaagaaggca
    12121 aaatcgtatt aacttctcgt aatactgaat taactgtcat cgacaccttc ggtcgtacca
    12181 aagaaaacta taaagtgcct tatggtgcgg tgctttctaa acacgacggt gcagaagtgg
    12241 ctgtgggtga agtggttgcg aactgggatc cgcacacaat gccggtaatc tcagaggtat
    12301 caggtcgtat ccaatttagc gacatcgtag atggcttaac cgttactcgc caaaccgatg
    12361 aattaaccgg tttatcatct atcgtggtgc aagatgtggg tgaacgtgca accgcaggta
    12421 aagatttacg tccggcatta cgcttagttg atagcaacgg caaagacatc ttaattccgg
    12481 gtacagacgt tgcagcacaa tacttcttac cgggtaaagc aatcgtaacc ttagacgatg
    12541 gtgcagaaat tgaagtcggt gaagccttag cccgtattcc acaagaatct gtggggacga
    12601 aagatattac cggtggtctt ccacgcgtag cagacttatt cgaagcccgt aaaccgaaag
    12661 agccggcaat cttggcggaa atttcaggta ttgtgtcgtt cggtaaagaa accaaaggta
    12721 aacgccgctt ggtaattact ccggcggaag gcgaagcgtt tgaagagatg attccaaaat
    12781 ggcgtcagct caacgtattt gaaggcgaga tggtacaacg tggtgatgtg atatcagacg
    12841 gtgcagaaac tccgcacgac atcttacgct tacgtggcgt tcacgccgtt accgactaca
    12901 tcgtaaacga agtgcaagaa gtttaccgct tacaaggggt aaaaattaac gacaagcaca
    12961 tcgaagtgat tgttcgccaa atgttgcgta aagctgtgat caccaacgct tacgactctg
    13021 aattcttaga gggcgaacaa gtggaagtgg ctcgtgtgaa aattgccaac cgcaaacgtg
    13081 ctgaagaagg caaaccgttg gtagaatttg aacgtgaatt gcttggtatt accaaagcat
    13141 ctcttgcaac agaaagcttt atttctgcag cctcgttcca agaaaccact cgagtgctta
    13201 ccgaagccgc agtggcaggt aaacaagatg aattgcgtgg cttaaaagag aacgtaatcg
    13261 ttggtcgctt aatcccggca ggtaccggtt ttgcttacca ccaagcacga gctaaaaaac
    13321 gtgcccaact ggaagcagga attgcagttg tccctgaatt tgaagcaccg gcagctaaag
    13381 ccaattcatt cgttaccgat gcagacatcg aagctgaatt tgaatttgtt gcagacgatg
    13441 caacccaaag tttagcagcg ttgttaaatg cgggtgagga agagtaatag ctcgtagggt
    13501 atgataaatt aaaaaatct
//
LOCUS       DANLUQ010000055        13509 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.055, whole genome shotgun sequence.
ACCESSION   DANLUQ010000055 DANLUQ010000000
VERSION     DANLUQ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13509
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.055"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(25..1564)
                     /locus_tag="PXB27_001267"
     rRNA            complement(25..1564)
                     /locus_tag="PXB27_001267"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(1767..1843)
                     /locus_tag="PXB27_001268"
     tRNA            complement(1767..1843)
                     /locus_tag="PXB27_001268"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1807..1809),aa:Pro,seq:tgg)
     gene            complement(1868..1943)
                     /locus_tag="PXB27_001269"
     tRNA            complement(1868..1943)
                     /locus_tag="PXB27_001269"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1908..1910),aa:His,seq:gtg)
     gene            complement(1973..2049)
                     /locus_tag="PXB27_001270"
     tRNA            complement(1973..2049)
                     /locus_tag="PXB27_001270"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2013..2015),aa:Arg,seq:ccg)
     gene            complement(2208..2930)
                     /gene="rffM"
                     /locus_tag="PXB27_001271"
     CDS             complement(2208..2930)
                     /gene="rffM"
                     /locus_tag="PXB27_001271"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HDL5346060.1"
                     /translation="MFDKVIIRGIALQAVKNREIFANWLMNEKRIQFGRLVAINAEKV
                     ILSEKNPLLRRVIDEAEFNYADGISVVQSIRKKYPNYKIERIAGADLWETLMQRAGAL
                     NVPVFLVGSSADTLTKVEAKLAQWKVNIVGRQNGYFKAEQEQDVIEQIKASGAKFISV
                     AMGTPKQELFIQQLQQQYPQALYMGVGGTYDVFAGKVKRAPKIWQNLGLEWLYRLLKQ
                     PTRWQRQLNLLKYAYYYLTNQL"
     gene            complement(2920..4191)
                     /locus_tag="PXB27_001272"
     CDS             complement(2920..4191)
                     /locus_tag="PXB27_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WzyE family oligosaccharide polymerase"
                     /protein_id="HDL5346061.1"
                     /translation="MNNLIPLGGFYLVSVGLFFSLLYQLYQKSGISFHLLFSLLYFVT
                     FYAGFPFSLIMAFGFDYPLPELKTQWQVFTAAGVGYLIYYVVYSRLWSAVSQPTALVA
                     NFQAKTTACILAAVAVVALLYFLYLNGFLLFKLEKYSQLFAKDRVILSALKRFFYFLL
                     PALLIFCFLYKNKTAWWALLIVGGAFGILSYVAVGGTWANLALAVGFFLLIGLQQGYL
                     SLSKVIAVSLIGVVAMFFLALARYKLTLSGSEMIYTFLYLSRDTFSPWENLARVFSNE
                     VEYQGLMPIVRDFYVYIPQSLWQERPEIVWNTANYFTKVILGNQSGLAISPTLLGSFY
                     IMGGFSVMALGMGIVAAMIKALDNLFAYAKACNSALLQAYCFGNLFNLIVLVREGIDA
                     FVSRFCFFTLIFMACYLLAKILSMGKNNNVR"
     gene            complement(4184..5254)
                     /locus_tag="PXB27_001273"
     CDS             complement(4184..5254)
                     /locus_tag="PXB27_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HDL5346062.1"
                     /translation="MLPIYHILGSEIEHHNQQVLTFFQQELLPLLPDSEHFFYVVGKP
                     SLQTAFPHLRIKQFSSQKAIANALIAKKRQNPTACFLLHGQFNPWIWLAIALGRLPTT
                     NLIWHIWGADLYEMATSWKFKLFYPFRRVAQQKILQVWATRGDLDHLWRNMRTRSEQD
                     HLIYFPTKLGDQIKISPTQNDRLTILLGNSGDPANELIQALSEIHQRLGNNVRIIIPM
                     GYPENNESYIRQVEAYSKRLFLAENLQILRKKLSFEEYVTLLSHCDLGYFNFKRQQGI
                     GTISLLISHNVPCVLHSANPFGLDLQAEKVPYLLAENLTASQIQTTKQQLKSIDKNRI
                     AFFAPGYTKLWQQRLIELMQNE"
     gene            5441..6862
                     /gene="glnA"
                     /locus_tag="PXB27_001274"
     CDS             5441..6862
                     /gene="glnA"
                     /locus_tag="PXB27_001274"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HDL5346063.1"
                     /translation="MSNAIANVSKLIQDNDIKFVLLKFTDIKGKEHGVSLPVSLVAND
                     LEDLFEEGKMFDGSSVEGWKAINKADMLLMPIAETAVVDPFARIPTLSIRCSIYDPNT
                     MQSYDRDPRSIATRAENYLKSTGIADSVMFGPEPEFFLFDDVRFSTEMNNVSYRIDDV
                     EAAWNTNRKFEEGNKAYRPLKKGGYCAVAPIDTAHDIRSEMCLLMEEMGLVIEAHHHE
                     VATAGQNEIATRFNSLTLKADETQIYKYIVQNVAVEHGKTACFMPKPFAGDNGSGMHC
                     NMSLSKDGKNVFMGDKYAGLSETALYYIGGIIKHAKALNAFTNPTTNSYKRLVPGFEA
                     PVLLAYSASNRSASIRIPAVTSPKATRVEARFPDPMANPYLCFAALLMAGLDGVINKI
                     HPGDAMDKNLYDLPPEELKDIPTVASSLESALDALSNDFEFLTQGGVFTKDFIDAYIG
                     IRRKEVERVNMTPHPVEFELYYS"
     gene            complement(6906..8049)
                     /gene="rffA"
                     /locus_tag="PXB27_001275"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /pseudo
     CDS             complement(6906..8049)
                     /gene="rffA"
                     /locus_tag="PXB27_001275"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251256.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
     gene            complement(8036..8680)
                     /gene="rffC"
                     /locus_tag="PXB27_001276"
                     /gene_synonym="wecD"
     CDS             complement(8036..8680)
                     /gene="rffC"
                     /locus_tag="PXB27_001276"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HDL5346064.1"
                     /translation="MKPDQWLSDFFGREIVQVKVAADDFRQIARLQKQGFVLVEGELE
                     FELALATFSENMTACRPADMADLPDLNLLFGSAFPDSRFRSPYFSREENQRFYRTWIE
                     NAVKGKFDDIGLVQCNADGQIQGAVTVRLLAQQQAKIGLLAVKPDCRQQGIGSELLNA
                     AIAWAKQQNAKKLMITTQLSNQKAIRFYQAFGASIKAVYYWFYPALEKYDDEQS"
     gene            complement(8677..9924)
                     /gene="wecC"
                     /locus_tag="PXB27_001277"
     CDS             complement(8677..9924)
                     /gene="wecC"
                     /locus_tag="PXB27_001277"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HDL5346065.1"
                     /translation="MMSKTEFNTICILGLGYIGLPTSVVFANAGKQVIGVDINPQVVA
                     SLNQGNIHIIEPDLQTAFTQAVKKGNFFATAKPQPADAFIIAVPTPLCGKQPDLTYIQ
                     NAAAMIAPCLRKGNLVVLESTSPVGTTENLAEWLSQARPDLAFPADVSIAYCPERVLP
                     GNIMTELVENDRIIGGLSEQCSQQAVDLYQIFAKGRCVTTTARTAEMCKLVENSFRDV
                     NIAFANEISMICDALKINVWELIELANLHPRVNILQPGAGVGGHCLAVDPWFIVSTSP
                     QQARLIRTAREVNDSKPQWVIEQVKTSVADCAMEQDCKPSELTIACLGLAFKPNIDDL
                     RESPALAITRQLAAWHQGSLLAVEPNIQQLAGEKITLVDLNTALSQADILVLLVDHTS
                     FKQISREQIRQRHIIDCRGVWVR"
     gene            complement(9965..10777)
                     /locus_tag="PXB27_001278"
     CDS             complement(9965..10777)
                     /locus_tag="PXB27_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS chain length-determining protein"
                     /protein_id="HDL5346066.1"
                     /translation="MKTLFYRLLVLILFAAIGAGAGYGLSYTQPEKWKVTAQFEQPTI
                     PDLGNYYSLLSTYHFLNSNSQEANSQTAQQQASEQVYAEFKRNLTSADLLQAYLLQTE
                     AVKLKAQVAKKSPAVVAAELVEQFRFEKDMATSADNVSLIAENAEEAQKLLADFIPLV
                     NTQARDKLNADLIAKWKVLFQQIKTASELNINGKQGSQDWAAKLQMMRSVQPLDNQLT
                     AYRLVKSPSVPLKSESPDPPILADDWGIKWLTAGLYAQFCGESESSTCGTLI"
     gene            complement(10788..11852)
                     /gene="wecA"
                     /locus_tag="PXB27_001279"
     CDS             complement(10788..11852)
                     /gene="wecA"
                     /locus_tag="PXB27_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HDL5346067.1"
                     /translation="MWLTFLTVFIVSFLALIVMRPVAEFVGLVDKPNFRKRHQGLIPL
                     IGGIALFIGNLTFYFMQWQDMRLPELYLTAVTILLIIGVLDDRFDISPLLRAGIQAAL
                     AGAMIYSGLSLSSLGQIIAPFSIELGWLGIVFTVFITIGVINAFNMVDGIDGLLAGLS
                     SVSFAGLGTLMFINGEPSLGYWCFALIFVLLPYAMFNLSLFGAKWKVFMGDSGSMLIG
                     FTIIWILLLSTQGQGSPISPITGLWLIAVPLIDMVAVIIRRLKKGKSPFKPDRLHLHH
                     LMMRAGLTSRQALLVITLWAGFCSTIGVLGEVYYWNQWVMMLMFIGLFFLYAYSITHA
                     WRVTRFVRRLKRRAKRSKQL"
     gene            11994..13274
                     /gene="hemL"
                     /locus_tag="PXB27_001280"
     CDS             11994..13274
                     /gene="hemL"
                     /locus_tag="PXB27_001280"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005721785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HDL5346068.1"
                     /translation="MSISEQLFEQAQKVIPGGVNSPVRAFKGVGGTPVFIEKAEGAYI
                     TDSDGKQYIDYVGSWGPMVLGHNHPAIIDAVLKAVPKGLSFGAPTAAEITLAELVCKL
                     VPSIEMVRMVSSGTEATMSAIRLARGYTNRDKIIKFEGCYHGHSDSLLVKAGSGALTL
                     GQPSGPGVPADFAKHTLTCTYNDLDSVKAAFEQYPNEIACLIVEPVAGNMNCIPPQEG
                     FLQGLRDLCTQYGAVFIIDEVMTGFRVALGGAQSYYGVTPDLTTLGKIIGGGMPVGAF
                     GGKKEIMEYIAPTGPVYQAGTLSGNPIAMAAGLACLTELSKAGNETILAEKTKTLAEG
                     FKALADKHNVPLTVQYVGGMFGLFFTEQKAVNNFQEVMKCDAAKFNRFFHLMLEQGVY
                     LAPSAFEAGFMSLAHSDEDIARTLEAADKAFAQL"
BASE COUNT         3890 a         3268 c         2747 g         3604 t
ORIGIN      
        1 acttgctgtc gttctcgatt ttggtaagga ggtgatccaa ccgcaggttc ccctacggtt
       61 accttgttac gacttcaccc cagtcatgaa tcataccgtg gtaaacgccc ccctttcggt
      121 taagctatct acttctggta caacccactc ccatggtgtg acgggcggtg tgtacaaggc
      181 ccgggaacgt attcaccgca acattctgat ttgcgattac tagcgattcc gacttcatgg
      241 agtcgagttg cagactccaa tccggactta gacgtacttt ctgagattcg ctccccatcg
      301 ctggctcgcc gccctctgta tacgccattg tagcacgtgt gtagccctac tcgtaagggc
      361 catgatgact tgacgtcatc cccaccttcc tccggtttat caccggcagt ctcctttgag
      421 ttcccgacct aatcgctggc aacaaaggat aagggttgcg ctcgttgcgg gacttaaccc
      481 aacatttcac aacacgagct gacgacagcc atgcagcacc tgtctcatgg ttcccgaagg
      541 cactctcata tctctacaag attccatgga tgtcaagagt aggtaaggtt cttcgcgttg
      601 catcgaatta aaccacatgc tccaccgctt gtgcgggccc ccgtcaattc atttgagttt
      661 taaccttgcg gccgtactcc ccaggcggtc gatttatcac gttagctacg ggcaccagag
      721 ttaaacccca atccccaaat cgacagcgtt tacggcgtgg actaccaggg tatctaatcc
      781 tgtttgctcc ccacgctttc gcacatgagc gtcagtacat tcccaagggg ctgccttcgc
      841 cttcggtatt cctccacatc tctacgcatt tcaccgctac acgtggaatt ctacccctcc
      901 ctaaagtact ctagttgtcc agtctgaaat gcaattccca ggttaagccc ggggctttca
      961 cacctcactt aaacaaccgc ctgcgtgccc tttacgccca gttattccga ttaacgctcg
     1021 caccccccgt attaccgcgg ctgctggcac ggagttagcc ggtgcttctt ctgtgattaa
     1081 cgtcaatcga ctgttctatt aaaacaatcg ccttcctcgt caccgaaaga actttacaac
     1141 ccgaaggcct tcttcattca cgcggcatgg ctgcatcagg gttcccccca ttgtgcaata
     1201 ttccccactg ctgcctcccg taggagtctg gaccgtgtct cagttccagt gtggctggtc
     1261 atcctctcag accagctaga gatcgcgggc ttggtgagcc tttacctcac caactaccta
     1321 atcccacttg ggctcatctt atggcaggtg gccaaatggt cccacccttt agtcctaaga
     1381 cattacgcgg tattagctac cgtttccagt agttatcccc ctccataagc cagattccca
     1441 agcattactc acccgtccgc cactcgtcac ccaaggagca aactcctatg tgctaccgtt
     1501 cgacttgcat gtgttaagcc tgccgccagc gttcaatctg agccatgatc aaactcttca
     1561 attcaaaaag tttaatcgct caatactgct gacataaaat cgcacttcca aatttaaacc
     1621 taattaaaat taagttaaaa gaaagtaatg aatttctagt tagcacctat taagacttca
     1681 aaattaaaaa aatgtttttc aatcaagtca atcaacaagt gcccacacag attgtctgat
     1741 aaattgttaa agaacaaaaa ataaattggt cggcgagata ggatttgaac ctacgaccca
     1801 ctggtcccaa accagttgcg ctaccaagct gcgctactcg ccgtctgata ctcacaagag
     1861 tattgaatgg ggtggctaat gggattcgaa cccacgacaa ctggaatcac aatccagggc
     1921 tctaccaact gagctatagc caccattgtg ttgatagtgc cgcattgaca tctggcgcgc
     1981 tcgacaagat tcgaacttgc gacctttggc tccggaggcc aacgctctat ccaactgagc
     2041 tacgaacgct tcgatttaca tcaattcaac ttaatgcgtc gcaacggagg cgtatattat
     2101 agatttcact ttcccttgtc tagcactttt taaaaaaaat gtaaaaaaat gtattgtttg
     2161 gttttatttt attcaaaacg tttaataaat atcaaaaaat gcgtttttta gagctgattt
     2221 gtcagatagt aataggcata ttttagtaaa tttaattgtc tttgccagcg ggttggctgt
     2281 ttcaatagtc gataaagcca ttccagcccc aaattttgcc aaattttagg tgctcgtttc
     2341 actttaccgg caaacacatc gtaagtcccg ccaacaccca tatataaggc ttgtggatat
     2401 tgttgttgta actgttggat aaaaagttcc tgttttggag ttcccattgc cacactaata
     2461 aacttcgcgc cacttgcttt aatttgctct ataacgtcct gttcttgctc cgccttaaaa
     2521 tagccattct gcctaccgac aatattcacc ttccattgtg ccaattttgc ttccaccttc
     2581 gttagggtat ccgccgagct gccgaccaaa aacaccggca catttaacgc tccggctctt
     2641 tgcattaagg tttcccataa atcggctccg gcaattcgct ctattttata gtttggatat
     2701 ttttttcgaa tcgattgaac aacactgatg ccgtctgcat agttgaactc ggcttcatct
     2761 atcactcggc gtaacaacgg atttttttcc gacagaatca ctttctcagc attaattgcc
     2821 actaatcttc cgaactgaat ccgcttttca ttcatcagcc aatttgcaaa aatttcccga
     2881 tttttgaccg cttgtaacgc aatgccccga atgatcactt tatcgaacat tattattttt
     2941 ccccattgaa aggattttcg ccagcaaata gcaagccata aatatcagcg tgaaaaagca
     3001 aaaacgggaa acaaatgcat cgattccctc tcgcactaat acgatcaaat tgaataaatt
     3061 gccgaagcag taggcttgca acaaggcgga attgcacgct ttggcgtaag cgaataggtt
     3121 atctaaggct ttaatcatgg cagctactat ccccattccc aacgccataa cgctaaatcc
     3181 gcccataata tagaaagagc cgagtaaggt aggcgaaata gctaaccctg attggttgcc
     3241 taaaatcact ttggtaaaat aattcgccgt attccagacg atctcagggc gttcttgcca
     3301 taaagattgt gggatatata catagaaatc ccgcacaatc ggcattaaac cttgatattc
     3361 cacctcatta gaaaatacac gagccaaatt ttcccaaggc gaaaaagtat ctcggcttaa
     3421 atataagaag gtgtaaatca tctcgctacc ggaaagggta agtttgtaac gggctagagc
     3481 gaggaagaac atcgccacca cgccgattag gcttacggca atcacctttg ataacgacag
     3541 atagccttgc tgcaatccaa tcaataagaa aaagccaacc gctagtgcta aatttgccca
     3601 tgtgccgccc actgccacat agcttaatat gccgaatgcc ccaccaacaa tcagtaacgc
     3661 ccaccacgct gttttattct tatataggaa gcaaaaaatt aacaacgccg gcaataaaaa
     3721 gtagaaaaat cgttttaatg ccgaaagtat gaccctatct ttggcaaaca gttggctata
     3781 tttttcgagt ttgaaaagca gaaaaccgtt taaataaagg aaatatagca gggcaacaac
     3841 tgccacagcg gcaaggatac aagcggtcgt ttttgcctga aaatttgcaa caagagcagt
     3901 tggttgcgac acagcactcc acaagcgtga atagacaacg taataaatca aatagccaac
     3961 acctgctgcc gtaaaaactt gccattgtgt ttttagctcc ggcagcgggt aatcaaagcc
     4021 aaatgccatt attaacgaaa acggaaaacc ggcgtaaaaa gtaacaaaat agagcaaact
     4081 aaacagtaaa tggaaagaaa tccccgattt ctgataaagt tgataaagta agctgaaaaa
     4141 tagcccgaca gaaacaagat aaaatccgcc aagtggtatt aaattattca ttttgcatta
     4201 attcgattaa tcgctgttgc catagtttag tgtagcccgg agcaaaaaac gcaatgcggt
     4261 ttttatctat gctctttagt tgttgtttgg ttgtttgaat ctgtgaagca gtcagatttt
     4321 ccgccaacaa ataaggcact ttttctgcct gtagatccaa cccaaacgga ttggctgaat
     4381 gtaacacaca aggtacatta tggctaatca aaaggcttat cgtgccaatg ccttgctggc
     4441 gtttaaaatt gaaatagccg agatcacaat ggctcaataa cgtaacatac tcttcaaagc
     4501 tcagcttctt ccgcaaaatt tgcaaatttt ccgccaaaaa taaccgcttg ctataggctt
     4561 ccacctgccg aatatagctt tcattatttt ccgggtagcc cattggtatg ataatccgca
     4621 cattgttccc tagtcgttga tgaatctcgc ttaaggcttg aataagctca ttcgctggat
     4681 cgcctgaatt gccgagcaat atcgtcaagc gatcattttg tgtggggcta atttttattt
     4741 gatcacctaa tttcgtgggg aaatagatta aatgatcctg ttctgatctt gtacgcatat
     4801 ttcgccacaa atgatccaga tcgcctcttg ttgcccaaac ctgtaagatt ttttgttggg
     4861 caacacgccg aaacgggtaa aacagcttaa atttccacga agttgccatt tcatataaat
     4921 ccgcccccca aatatgccaa atcaggttgg tggtcggcaa tctgcctaag gctatcgcca
     4981 gccaaatcca agggttaaac tgcccgtgta acagaaaaca agcggtcgga ttttgccgtt
     5041 tttttgcaat taaggcgttc gcaatcgctt tttgtgaaga gaactgctta atccgcaaat
     5101 gaggaaaagc agtttgcagt gaaggcttgc ctaccacata aaaaaaatgt tcggagtctg
     5161 gcaataacgg caaaagctct tgctgaaaaa aggttaaaac ctgttggtta tgatgctcaa
     5221 tctcggagcc tagaatatga taaatcggca acattacacg cttttctctc cccaccatac
     5281 aaacaatatg caagcggtaa gattttagca aaaatttgca acattactgc aacttctttt
     5341 agctttgaaa cactaaaaaa catcacacac ttggttaaaa aatgctatga taaccgagct
     5401 tttttattat tcatttatcc caacgaggac ttattttact atgtcaaacg caatcgctaa
     5461 tgtatctaaa ctgattcaag acaatgatat taaatttgtc ttattaaaat ttactgatat
     5521 taaaggtaaa gaacacgggg tttcgctccc tgtcagctta gttgccaacg atttggaaga
     5581 cttattcgag gaaggcaaaa tgttcgacgg ttcgtccgta gaaggttgga aggcaattaa
     5641 caaagcagat atgttgttaa tgccgattgc tgaaaccgct gtagttgacc ctttcgcccg
     5701 tattcctacc ctttctattc gttgttccat ttacgaccca aacacaatgc agtcttatga
     5761 ccgtgaccct cgctctattg caacccgtgc cgaaaactac ttgaaatcca ccggtatcgc
     5821 cgatagcgta atgtttggac ctgagcctga atttttctta ttcgatgatg tgcgttttag
     5881 caccgaaatg aacaatgtgt cataccgaat tgacgatgta gaagcggctt ggaataccaa
     5941 ccgtaaattt gaagaaggca acaaagccta tcgtccgttg aaaaaaggcg gctactgtgc
     6001 ggttgcaccg attgataccg cccacgatat tcgctccgaa atgtgtttat tgatggaaga
     6061 gatgggttta gtgattgaag ctcaccacca cgaagttgcg acagccggac aaaatgagat
     6121 cgcaacccga ttcaacagcc tgaccttaaa agccgatgaa acccaaatct ataaatacat
     6181 cgtgcaaaat gttgccgtag aacacggaaa aaccgcttgc tttatgccaa aaccgttcgc
     6241 cggcgacaac ggctcgggaa tgcactgtaa tatgtcatta agcaaagacg gcaaaaatgt
     6301 gtttatgggc gataaatacg ccggcttatc tgaaactgcc ctctactata ttggcggtat
     6361 cattaaacac gcaaaagcat taaatgcctt tactaaccca accacgaact cttataaacg
     6421 tttagtgccg ggttttgaag caccggtgtt attggcatat tctgcaagta accgctctgc
     6481 ctctatccgt attccggctg tgaccagtcc gaaagcgacc cgtgtagaag cacgcttccc
     6541 ggatccaatg gcaaacccat acctttgctt tgccgccctg ttaatggctg gcttagacgg
     6601 tgtgatcaac aaaatccacc cgggcgatgc aatggataaa aacttatacg atttaccgcc
     6661 ggaagagtta aaagatattc caacggttgc ctcgtcttta gaatccgctc ttgacgcatt
     6721 aagcaatgat tttgaattct taacacaagg tggcgtgttc acgaaagact ttattgatgc
     6781 ttatatcggt attcgtcgca aagaggtgga acgtgtaaat atgactccgc acccggtaga
     6841 atttgaactt tactattcat agtaacaaca tatagcaaaa aacggctgaa acactcagcc
     6901 gttttttatt gattgaagaa agcaagcact gcttgaataa cacgctcttg ctcctgctta
     6961 ctcaaattat aaaacagtgg caatcttacc aaccgttcgc tttctaaggt agtaaagcga
     7021 tcatcacctg ctaatcgacc taatttctgc cctgccggac tgctgtgcaa cggcacataa
     7081 tgaaacacgg ttaagatatt ctgtgccttc ataaaatcaa taaaagcagt tctctgcttc
     7141 aagtcactta gctttaagta gaacaaatgc ccgttatggc gacaatattc gccaatgcga
     7201 ggaagctcgc acttagctgt ttgggcaagt ggcaaaaagg cttgatagta attgtgccaa
     7261 agggttaaac gctgttggtt gattttatct gcctctataa gctgagcata caaataagcc
     7321 gcttgaattt ccgacattaa aaaacttgaa ccaatatcct gccagcgata tttatctacc
     7381 tcaccacgaa aaaattggct acggttagtg cctttctctc tgatgatttc cgctttttcc
     7441 agcaaatgcc tcattaatca cgatcgcccc accttcaccg ccagcggtat agtttttggt
     7501 ttcgtgaaaa ctatagcaac caatatgccc gatggaacct aacgccctac ctttataaaa
     7561 cgaattgact gcctgtgccg catcttctac cacataaagc ccgtattttt cggctgttgc
     7621 cattattgaa tccatatcac aagcaatgcc ggcataatgc accggcacaa tcactttggt
     7681 ttttggggta atggcttgct caattttgcg ttcatcaata ttcattgtat ccggacgaat
     7741 atccacaaac acaatctttg ccccacgcaa cgcaaacgca tttgcagtag agacaaaagt
     7801 atagctcggc ataatcactt catcgtccgg ctgaatatcc agcagtattg ccgccatctc
     7861 taacgcagcc gtgcaagaag gggtaagcaa cactttgcta cgggccaggt tttcaagcca
     7921 ctgctcacat aagcgggtga atttaccacc gcccgaaagc tgccctgttg ccatcgcctg
     7981 ttctatatag gcttgctccg cccccacaac cgggggttga ttaaacggaa taaaatcagg
     8041 attgttcatc atcgtatttt tccaaagcag ggtaaaacca ataatacacc gccttaatgc
     8101 ttgcaccaaa cgcttgataa aaacgaatcg ctttctggtt actcagctga gtggtaatca
     8161 tcagtttttt cgcattttgc tgtttcgccc acgctatcgc agcatttaac aattccgagc
     8221 caataccttg ttgtcggcaa tcaggcttta ccgccaataa tccgattttc gcttgctgtt
     8281 gggcaagtaa gcgaaccgtt accgcccctt gaatttgccc gtcagcattg cattgaacca
     8341 agccaatatc atcaaacttg ccttttaccg cattttcaat ccaagtccgg taaaagcgtt
     8401 gattctcttc ccttgaaaaa tagggcgaac gaaaccggct atcgggaaat gccgaaccaa
     8461 acaataagtt caagtcgggc aaatctgcca tatccgccgg gcgacaagcg gtcatatttt
     8521 cagaaaatgt tgcaagtgcc agctcaaatt ccaattctcc ctccacaagt acaaaacctt
     8581 gtttttgcaa cctagcgatt tgcctaaaat catcagccgc tacttttact tgcacaattt
     8641 ctctaccgaa aaaatcggac agccattgat ccggcttcat cttacccaca ctcctcgaca
     8701 atcaataata tgccgttgcc tgatctgttc ccttgaaatt tgcttaaatg aagtgtgatc
     8761 cactaacaag actaaaatgt ctgcctgcga aagtgcggtg ttgagatcga ctaaggtgat
     8821 tttttcgcca gccagctgtt gaatatttgg ctcaaccgcc aacaggctac cctgatgcca
     8881 agcggcaagt tgccgggtaa tcgctaatgc cgggctttcc cgcaaatcat caatattcgg
     8941 cttgaaagct agccctaaac aagcgatggt tagctcactt ggcttacaat cttgctccat
     9001 cgcacagtct gcgaccgatg ttttcacttg ctcgattacc cattgcggtt tgctgtcatt
     9061 cacttctcgt gccgtgcgaa taaggcgagc ctgttggggc gatgtggaaa cgataaacca
     9121 cgggtcaacc gccaaacaat gtccgcccac ccctgctccg ggctggagaa tatttactct
     9181 cgggtgaaga tttgccaatt caattagctc ccacacattg atcttcaacg catcacaaat
     9241 cattgaaatt tcattggcaa aggcaatgtt cacatcacga aagctgtttt ccaccaactt
     9301 gcacatctcc gctgtgcgag ctgtggtggt gacacaacgt ccttttgcaa aaatttggta
     9361 taaatcgacc gcttgttgac tgcattgttc gcttaatccg ccgataatgc ggtcattttc
     9421 aaccaattcc gtcattatat tacccggcaa aacacgttca gggcagtagg caatagacac
     9481 atcggctgga aaggctaaat cagggcgagc ttggcttagc cattcggcta aattttctgt
     9541 tgtaccgaca ggcgaagtgg actccagcac caccaaattc cctttacgca aacaaggggc
     9601 aatcatagcg gcggcatttt gaatataagt gagatcgggc tgcttcccgc aaagtggggt
     9661 tggcacggcg atgataaaag catcggccgg ttgcggtttg gcagttgcaa aaaaattacc
     9721 ttttttgacc gcttgtgtga aggcagtttg taaatccggc tcgataatat gaatgttgcc
     9781 ttgattaagg cttgccacca cttgcggatt aatatccacc ccaattactt gcttgccggc
     9841 attggcaaaa acgactgagg tcggcaagcc gatatagcct aaccccagaa tacaaatagt
     9901 gttaaattca gttttactca tcatcagtta caaagagaaa tggcacacta aattgtgtgc
     9961 cattttagat taaagttccg caggttgagc tttcgctttc accgcaaaac tgagcataaa
    10021 gcccagcagt aagccactta atgccccaat catcagccaa aataggcgga tcaggcgatt
    10081 ctgatttaag tggaacgcta ggcgatttaa ccaagcgata agctgtaagt tggttgtcta
    10141 acggctgaac agaacgcatc atttgcagtt tagctgccca atcttgtgag ccttgcttac
    10201 cgttaatatt caactccgaa gcagttttaa tttgctggaa caagactttc catttcgcaa
    10261 ttaaatcggc gtttaattta tctcgagctt gggtgtttac caacggaata aaatcggcta
    10321 ataatttttg agcctcttcc gcattttctg caattaagct gacattatct gccgaagttg
    10381 ccatatcttt ttcaaaacgg aattgttcaa ctaattcagc cgccaccacc gccggtgatt
    10441 tctttgcaac ctgagctttt aatttcaccg cttctgtttg tagcaaatag gcttgaagca
    10501 agtcggcgga cgtgaggttg cgtttaaatt ccgcataaac ctgctcactt gcctgttgct
    10561 gtgccgtttg gctattcgct tcttggctat tactattcaa aaaatggtag gtggaaagta
    10621 acgagtaata attacctaaa tcgggaatag tcggttgctc aaattgagcg gtcactttcc
    10681 atttttcagg ttgagtataa ctcagcccat aaccggcacc tgccccgata gcagcaaata
    10741 aaattagcac taaaagtcga taaaataagg ttttcacaac atatccttta aagttgtttg
    10801 ctacgttttg cacggcgttt tagacggcga acaaaacggg ttacacgcca agcgtgggta
    10861 atggaatagg cgtataggaa aaacagcccg ataaacatta gcatcatcac ccactgattc
    10921 caataataca cctcgcctaa taccccaatg gtggaacaaa atcccgccca gagggtaatc
    10981 accaataatg cttggcgaga ggtaagccct gctcgcatca ttaaatggtg taggtgcaag
    11041 cggtctggtt tgaacggact tttacctttt ttcaaacggc ggataatcac cgcaaccata
    11101 tcaattaacg gcacggcaat cagccacaaa ccggtaatcg ggctaattgg cgagccttgc
    11161 ccttgggtgc ttaacagcag aatccaaata atggtaaagc cgatcagcat actgcctgag
    11221 tcgcccataa acactttcca tttcgcccca aacaggctca aattgaacat tgcatacggc
    11281 aacaacacaa agataagggc aaagcaccaa tagcccaaac ttggttcgcc attaatgaac
    11341 attaaagtgc caagaccggc aaaactcacg ctggaaagcc ctgctaataa gccatcaatc
    11401 ccatctacca tattaaacgc attaataacg ccaatggtga taaacacggt aaagacaatg
    11461 ccgagccaac ctaattctat actaaagggg gcaataattt gccctaaact gctgagcgac
    11521 aagccactgt aaatcatcgc cccagctagt gctgcttgaa tcccggcacg cagtaatggg
    11581 ctgatgtcaa atcggtcatc taacacgccg ataatcagca aaatagttac cgctgtcaga
    11641 tacagttccg gcagccgcat atcttgccac tgcataaaat agaaagtgag attgccgata
    11701 aataatgcaa taccaccgat taacggaatc aacccctgat ggcgtttacg gaagttgggc
    11761 ttatctacca aaccaacaaa ttccgccacc ggacgcatca caatcagggc taaaaaagaa
    11821 acaataaata cggttaaaaa agtaagccac atacagggat tgcctcaaaa aatgagtcat
    11881 aaaataggaa tgcagaatag cataaggcaa caaaatcgta aattttttag cgaaaacaag
    11941 ggcttttcag tacaataaca ggcaaatttt tacatcaaaa gaaaggaatt gatatgtcta
    12001 tttcagaaca actgtttgaa caagcccaaa aagtgattcc gggcggtgtg aactcccctg
    12061 tgcgtgcatt taaaggcgtg ggcggcacac cggtttttat tgaaaaagcc gaaggtgctt
    12121 atattaccga cagcgacggc aaacaatata tcgactatgt cggctcttgg gggccaatgg
    12181 tactcgggca taaccacccg gcgattattg atgcggtgct aaaagccgtg ccaaaaggct
    12241 taagtttcgg tgccccaacg gcagcagaaa tcacacttgc cgaattagtg tgcaaattag
    12301 taccttctat tgaaatggtg agaatggtga gcagcggaac agaggcaaca atgtctgcca
    12361 ttcgtttggc tcgtggctat accaaccgag ataaaatcat caaatttgaa ggctgctacc
    12421 acgggcattc cgattctctg ttagtaaaag ccggttcagg tgctttaacc ctcggtcagc
    12481 caagcggccc gggcgtaccc gctgatttcg ccaagcatac gcttacttgc acctataatg
    12541 atttagattc agtaaaagca gcatttgagc aatacccaaa tgagatcgct tgtttaattg
    12601 ttgagcctgt tgccggcaat atgaactgca ttccgccaca agaaggcttt ttacaaggct
    12661 tacgtgatct ttgcacacaa tatggtgcgg tatttattat tgatgaagta atgaccggct
    12721 tccgtgtcgc actcggtggg gctcaatctt actatggggt aacaccggat ttaaccacac
    12781 tcggcaaaat tatcggcggc ggaatgccgg tgggggcatt tggtggtaaa aaagagatta
    12841 tggaatacat cgctccaact ggcccggttt accaagccgg cacgctctca ggcaacccga
    12901 ttgcaatggc tgcaggtttg gcatgcttaa ctgaattatc aaaagcagga aatgaaacca
    12961 ttttagcgga aaaaaccaaa accctcgctg aaggctttaa agctcttgca gataagcaca
    13021 atgtgccatt aacggtacaa tatgtcggcg gaatgttcgg cttattcttc accgaacaaa
    13081 aagcagtaaa caacttccaa gaagtaatga aatgtgatgc cgccaaattc aaccgtttct
    13141 tccacttaat gctggaacaa ggcgtgtatt tagccccatc ggcattcgag gcagggttta
    13201 tgtctttagc tcacagtgat gaagatattg ctcgtacgct agaggctgcg gataaggcat
    13261 ttgctcagct ttagaagaat gacaagcggt tgggtttact caataatttg caagtttttg
    13321 agggaatttg accgcttgta gtgcaaattt ctcttacgat agcaattatg atggtgttcg
    13381 tctctgacga gtgcttatac aaactttcat tatgcttaaa caagcacccg tctggagacg
    13441 ggcgctatcg agggaatagg attgataaag caatatatgg gcttccaaca cttgcaggca
    13501 aggacaagt
//
LOCUS       DANLUQ010000056        13444 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.056, whole genome shotgun sequence.
ACCESSION   DANLUQ010000056 DANLUQ010000000
VERSION     DANLUQ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13444
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.056"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..778
                     /locus_tag="PXB27_001281"
     CDS             <1..778
                     /locus_tag="PXB27_001281"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDL5346069.1"
                     /translation="ETDHDVSVWAARYIVDRINAFQPTKEKPFVLGLPTGGTPEKTYK
                     ELIKLYQAGEVSFKNVVTFNMDEYVGLPKEHPESYHSFMFKHFFDHVDIPLENINILN
                     GMAEDVNAECERYEEKIRFYGKIHLFMGGVGVDGHIAFNEPASSLSSRTRIKTLTEDT
                     RIANSRFFNNDVSQVPKFALTVGVGTLLDAEEVMILVTGHNKALALQACVEGAVNHLW
                     TISCLQLHRRGIVVCDEPATQELKVKTVKYFKELEKNVAR"
     gene            859..2001
                     /gene="nagA"
                     /locus_tag="PXB27_001282"
     CDS             859..2001
                     /gene="nagA"
                     /locus_tag="PXB27_001282"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDL5346070.1"
                     /translation="MNHYALTNCVIYTGEQVLYQHALVVKEDKIDSIVLEENLPLDLQ
                     KIDLKGANLSAGFIDLQLNGCGGVMFNEEITTRTLEIMQETNLRSGTTSYLPTFITSP
                     DEGVKQAVAVMREYLSKHKNQALGLHLEGPYLSVAKKGVHRPEFIREISPEMLDFLCD
                     NGDVITKITIAAENPTADHIKRFNDAGIIVSIGHSNATFAQTKQRIAEGAAFATHLHN
                     AMSPVSNGREGGVVGAVLDSDIYTGIICDGLHVEWGNIRIAKKAKGDKLVIVSDAVAA
                     AGSDIESFIFVGKKVTVKDGKCYDEFGALGGAAITMIESIRNVVQHCDISLNEAIRMS
                     TLYPAKAIGVEETLGSLEAGKIANLAIFDNQFEVKATAVNGQLQWH"
     gene            2083..2397
                     /locus_tag="PXB27_001283"
     CDS             2083..2397
                     /locus_tag="PXB27_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HDL5346071.1"
                     /translation="MQFKDYLATFPTIDHLAGLNVINSGEVIQHIPAIEGKLGSLKLY
                     NALFAKFDGKLTASAAKQGLEWFAEHVEDAKNNVGKHPNIDLLFRVIEQDLSLSLEPI
                     VK"
     gene            complement(2476..3666)
                     /locus_tag="PXB27_001284"
     CDS             complement(2476..3666)
                     /locus_tag="PXB27_001284"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDL5346072.1"
                     /translation="MTQAIESVLAELKRGVENIYSEEDLITKLKENRPLRIKLGADPT
                     APDIHLGHTVVLNKLRQFQQFGHEVYFLIGDFTGMVGDPSGKNSTRPPLSREDVLRNA
                     ETYKEQIFKILDPQKTKIVFNSEWLGKLGTEGMIRLASNYTVARMLEREDFKNRFTNQ
                     QPIAIHEFIYPLLQGHDSVELKADVELGGTDQTFNLLVGRELQKSAGQKPQVAMTLPL
                     LVGLDGEKKMSKSLGNYIGVTEAPSEMFGKIMSISDDLMWDWYNLLSFRPLDEIAQLK
                     ADVENGKNPRDVKILLAKEIIARFHDEAAADAAEQEFINRFQKGAIPDEVPEFTFEGE
                     IGLAALLKEAGLVPSTSEAIRSAQQGGVKIDGEKVEDVKQTAQKGTFVYQVGKRKFAR
                     ITVK"
     gene            complement(4029..5333)
                     /locus_tag="PXB27_001285"
     CDS             complement(4029..5333)
                     /locus_tag="PXB27_001285"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HDL5346073.1"
                     /translation="MESIIALFKGMGLMHLELGQAIMIVISLVLLWLAIARKFEPLLL
                     LPIGFGGLMSNIPEAGMAMTALDNLLHSGSAEQLAIIAAKVNSTSLDLHSIKDAIALA
                     APSVQNELEVMAGDMGYTAGVLALFYKVAIGYGVAPLIIFMGVGAMTDFGPLLANPRM
                     LMLGAAAQFGIFATVLGALGLNWLGIIDFTLPQAAAIGIIGGADGPTAIYLASKLAPE
                     LLGAIAVAAYSYMALVPLIQPPIMKALTTEQERKIRMVQLRNVSNREKIIFPIVLLLL
                     VALLLPDAAPLLGMFCFGNLLRTSGVVERLNDTAQNALINIVTIFLGLSVGAKLVADK
                     FLQPQTLGILVLGVIAFGIGTASGVIMAKIMNRFSQHKINPLIGSAGVSAVPMAARVS
                     NKVGQEYDKQNFLLMHAMGPNVAGVIGSAIAAGVMLKYISTM"
     gene            complement(5343..7169)
                     /gene="oadA"
                     /locus_tag="PXB27_001286"
     CDS             complement(5343..7169)
                     /gene="oadA"
                     /locus_tag="PXB27_001286"
                     /EC_number="7.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-extruding oxaloacetate decarboxylase
                     subunit alpha"
                     /protein_id="HDL5346074.1"
                     /translation="MTTQPKKIAITDVVLRDAHQSLFATRLRLDDMLPIASELDNIGY
                     WSLETWGGATFDSCIRFLGEDPWVRLRELKKAMPKTPMQMLLRGQNLLGYRHYADDVV
                     DKFVERSVANGMSVFRVFDALNDPRNMQAALQAVKKHGGHAQGTLSYTTSPVHTLQTW
                     LDVTEQLLEIGVDSLVIKDMSGILTPMAAYELVSEIKKRYDVELHLHCHSTTGMAEMA
                     LLKAIEAGVDGVDTSISSMSGTYGHPATESIVATLQGTPYETGLSIPALEKISAYFRN
                     VRKKYAKFEGQLRGIDSRILVAQVPGGMLTNLESQLKQQNASDKLDLVLQEIPRVRED
                     LGNIPLVTPTSQIVGTQAVMNVLMGERYKTIAKETAGILKGEYGRTPAPVNKALQDRV
                     LEGAEPITDRPADHLPPELEKLVAEVTASAKEKGITLSENMIDDVLIVALFQQVGWKY
                     LENRNNPSAFEPVPSADEAKPAQASAASAPKAQTQSGSAIYTVELEGKAFVVKVSEGG
                     DITNIAPTPATPAPQAVDSAPKSANTLSANAEPVNAPMAGNILKVEVKEGQQVAEGDV
                     LLILEAMKMETQICAAKSGTIQGINVKQGDVVAVNDTLMSIA"
     gene            complement(7209..7463)
                     /locus_tag="PXB27_001287"
     CDS             complement(7209..7463)
                     /locus_tag="PXB27_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit gamma"
                     /protein_id="HDL5346075.1"
                     /translation="MAPAELFAEGINLMVAGMGFVMLFLLVLIYAISFISKIINQYFP
                     EPVSIPASKTVPQSPPDDLERLRPVIAAAIAHHRRINGQH"
     gene            complement(7653..8840)
                     /locus_tag="PXB27_001288"
     CDS             complement(7653..8840)
                     /locus_tag="PXB27_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HDL5346076.1"
                     /translation="MSNNNRSALLGAAFLMATSAIGPGFLTQTATFTQTLLASFGFVI
                     LLSILLDIGAQLNIWRIVAVSEKKAQDIANAVLPGAGYFLALLIVMGGLAFNIGNVGG
                     AGLGLNILTGLSPEMGAVVSGAIAVGVFLFHEAGKVMDRFAQIMGFVMIALTIFVAVK
                     ANLPIDDAVVHTFVPEKLDVIAIVTLVGGTVGGYITFAGAHRLLDAGIKGKENLKEVS
                     KSSVSAILIASIMRVVLFLAVLGVVSMGVQLDPKNPAATPFAHVAGDVGLMIFGVVIW
                     AASITSVIGAAYTSVSFITSFSPFIEKHKNCFIVAFIVVSTAVLATIGRPAQVLVFVG
                     TLNGLILPISLGLILLAAHKTKIVGDYKHPVWMTVSGAIVVILMAVLSAMTLSKYISG
                     LMA"
     gene            complement(9115..9852)
                     /gene="pxpA"
                     /locus_tag="PXB27_001289"
     CDS             complement(9115..9852)
                     /gene="pxpA"
                     /locus_tag="PXB27_001289"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HDL5346077.1"
                     /translation="MNHVDLNVDLAEGCGNDHSLLQLVTSANVCCGLHAGDYNEMRKA
                     INWAKENSVRVGAHPSFPDRENFGRTNMQLPDDELKACLLYQLGAAKALCDAENMPMS
                     YVKPHGALYNMAAKDPHLATLIANTVKQFDPNLKLMGLSGSLMLSIAEQHGLETISEV
                     FADRHYLADGSLVPRTRDDAMVESDDEAISQVLQMVLDGTVPTVDGGNVAIKADSICL
                     HGDGEHAVVFAERIKKELQAKGILVKA"
     gene            complement(9839..10768)
                     /locus_tag="PXB27_001290"
     CDS             complement(9839..10768)
                     /locus_tag="PXB27_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HDL5346078.1"
                     /translation="MIYIEIIQGFAHIQDLGRYGYRHLGVSHGGAMDSLALQAGNLLL
                     KNANNAPAIELTLGGMRITFDCDTPFCLTGALVEAELDGTPVFSYYRYTANARQTLTI
                     KRIVLGNYAYLCVAGGFLVPQVLGSASTALKAQFGGFQGRMLKAGDNIATGRRDSRLS
                     LMSIEPIDFTSRIRATASSEYEAFTADSRERFWQQGWQLQNNSNRMGYRFAEKALELT
                     ASLEMLSYAAPSGTVQVPPDGQPIVLMADAQTTGGYPKIACIIQADLGRLAQNPFGST
                     VQFEQVSREQAVEIYQKDQNYLETIRRKANESR"
     gene            complement(10765..11412)
                     /gene="pxpB"
                     /locus_tag="PXB27_001291"
     CDS             complement(10765..11412)
                     /gene="pxpB"
                     /locus_tag="PXB27_001291"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HDL5346079.1"
                     /translation="MLTIHHTSEHSLLCLAEPPAELAKQQQIWRVAEQARALDGVLEV
                     VVGMNNFTLFHAIGTDSTRLIQAVQQLWENDQAQAAQSPRLIEIPVHYGGEFGEDLFE
                     VAQFHNTTPEEIIRRHTEPTYTVFMIGFQPGFPYLNGLPQNLHTPRRNVPRTKVPAGS
                     VGIGGRQTGIYPFTSPGGWQLLGRTELPLFDIQHNPPTLLTAGDNVRFVAESITL"
     gene            complement(11506..12330)
                     /locus_tag="PXB27_001292"
     CDS             complement(11506..12330)
                     /locus_tag="PXB27_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HDL5346080.1"
                     /translation="MKKLLLFLLFIGNPIFAKSPELMLVEQYQDQDISGWVMSEKLDG
                     VRGFWDGQQLISRQGYPLNPPDYFIQDFPPFAIDGELFSERGKFEEISSIVRSAQPKG
                     WHKLKLQVFDVPNAQGNLIERLATLENYLKQHPTLHIAIIEQIPIKDKAHLQQFYQSV
                     LEQGGEGVIVRNPNAGYIQGRSAQILKIKPVLDEECTVVAHHKGKGKYTDKLGAITCE
                     NQRGRFRIGSGFKDKERENPPPIGSIITYKYRGVTESGKPRFATFWRIRNDQISEN"
     gene            complement(12464..13288)
                     /locus_tag="PXB27_001293"
     CDS             complement(12464..13288)
                     /locus_tag="PXB27_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346081.1"
                     /translation="MEHFIGLGVAGNFAGHLEQAGEEADFAKVKTVEAVQPKAIFPFY
                     VPAENLGDYQFLSTYPLSNTAINFPSDADNLQIEPEVALICEIGYQDQQVVSLTPTHF
                     AAYNDCSIRRPNANKICEKKNWGASSKGLSAIHLPLDNFNKGGTLDNYNIACFHKRNG
                     ELNTYGIDSPAVEYSYFHQKLLDWIVERMNNQPDEGPMNNISEMLKQANYPTKAIISI
                     GATRYTPFGESNLLQVGDVSMVVVYNVKKYSHSQIEEMAKLETFSEDISALIQTVR"
BASE COUNT         3669 a         2990 c         3022 g         3763 t
ORIGIN      
        1 agaaactgat cacgatgtga gtgtttgggc tgctcgttat attgttgatc gtatcaatgc
       61 attccaacca accaaagaaa aaccttttgt gcttggctta ccaaccggcg gtacgcctga
      121 aaaaacctat aaagaattaa ttaaattata tcaagctggt gaagtgagct ttaaaaatgt
      181 ggtgactttc aatatggacg aatacgtcgg cttacctaag gaacaccctg aaagctacca
      241 cagttttatg ttcaagcact tttttgacca cgttgatatt ccgcttgaga atatcaatat
      301 cctcaacggt atggcggaag acgtgaatgc agaatgcgaa cgttatgaag aaaaaatccg
      361 tttttatggt aaaatccatc tgtttatggg cggggtcggt gtggatgggc atattgcgtt
      421 taatgagcct gcatcttcgc tttcttcccg cactcgaatt aaaacgctta ccgaagatac
      481 tcgcattgca aactcacgtt tctttaacaa tgacgtaagc caagtgccga aatttgcttt
      541 aaccgttggg gttggtacgt tgcttgatgc cgaagaagtg atgatcttag tgaccggcca
      601 taacaaagcc ttagcattac aggcgtgtgt ggaaggggcg gtaaatcatc tttggaccat
      661 cagttgctta caattacatc gccgtggtat agtggtgtgt gatgagccgg caacccaaga
      721 gctgaaagtt aaaacagtta agtatttcaa agagttagag aaaaacgtag ctcgctaatt
      781 ctgattttac aagcggttaa ttttgtaata ttttttacaa aaacgaccgc ctgttagcat
      841 ttaaagagaa gagagaaaat gaaccattat gccttaacaa actgtgtgat ttacaccggt
      901 gaacaagtgc tttaccaaca cgcattagtg gtgaaagaag ataaaatcga ctccattgtt
      961 ttggaagaaa atttaccgct ggatctgcaa aaaatcgacc taaaaggagc gaatctttca
     1021 gccggtttta ttgacttgca actgaacggc tgcggtgggg tgatgtttaa tgaagaaatc
     1081 actacccgca ctttagaaat tatgcaggaa accaatctgc gttcaggcac aaccagctat
     1141 ttaccgactt ttattacctc gcctgatgag ggagtgaaac aagcggtggc ggtaatgcgt
     1201 gagtatttgt cgaaacacaa aaaccaagca ctcggcttgc atttggaagg accttatcta
     1261 agtgtggcaa agaaaggagt acaccgtccg gaatttatcc gtgaaatcag ccctgaaatg
     1321 ttagatttct tatgcgataa cggcgatgta attacgaaaa tcacgattgc agcagaaaac
     1381 ccgaccgcag accatatcaa acgctttaat gatgcaggaa ttattgtatc tatcgggcat
     1441 tccaatgcca cttttgctca aaccaaacag cgaattgccg aaggggcagc gtttgctacg
     1501 catttacaca atgcaatgtc gccggtcagc aacgggcgtg aaggcggtgt agtaggggcg
     1561 gtgttggata gcgatattta caccggcatt atttgcgatg gcttgcacgt tgaatggggc
     1621 aacattcgca ttgccaaaaa agccaaaggc gataaattag tgattgtcag cgatgctgtt
     1681 gctgccgcag gaagtgatat tgagtcgttt atttttgtcg gtaaaaaagt cacggtgaaa
     1741 gatggcaaat gctacgatga attcggagca ttgggcggtg cggcaatcac gatgatcgaa
     1801 tccattcgca acgtagtgca acattgcgat attagcctga atgaagcaat ccgaatgagt
     1861 accctttacc cggcaaaagc gattggggta gaggaaacac tcggctcact tgaagccggc
     1921 aaaattgcca accttgcgat tttcgataat caatttgaag taaaagcgac tgctgtaaac
     1981 gggcaattac agtggcatta attttgctac aaattctaag cataacagca tataatccaa
     2041 aggcaggcat tgtgtctgcc ttttatttat agggagaata acatgcaatt taaagattac
     2101 ttagccactt ttccaactat tgatcatcta gccggcttaa acgtgataaa tagcggtgaa
     2161 gtgattcaac atattcctgc gattgaaggt aaattaggtt cactaaaact ttacaatgca
     2221 ctgtttgcga aatttgatgg gaaattaacc gctagtgcag caaaacaagg gttggaatgg
     2281 tttgccgaac acgttgaaga tgcgaaaaat aatgtgggta agcacccgaa tattgatcta
     2341 ttatttaggg taattgagca agatttatct ttaagtttag agccgattgt taaataagat
     2401 tgcactagtg aaaaataaca agcggtcatt atctgcaaaa tttttgcaaa taatgactgc
     2461 ttgtgtttaa gaggcttatt tcacggtaat gcgtgcaaat ttgcgtttgc cgacttggta
     2521 aacaaaagtg cctttttgag cagtttgttt cacatcttcc actttttcac catcaatttt
     2581 aaccccgcct tgttgggctg agcgaattgc ctctgaagtg gacggtacta agccggcttc
     2641 tttcagtaga gccgctaggc cgatttcacc ctcaaaggta aattcaggca cttcatcagg
     2701 aattgcccct ttttggaaac ggttgataaa ctcttgctct gctgcatctg ctgccgcttc
     2761 atcgtggaaa cgagcgataa tttcttttgc cagtagaatt ttcacatcac gcgggttttt
     2821 gccattttca acatccgctt ttaattgtgc aatttcgtct aatggacgga atgagagcag
     2881 gttgtaccaa tcccacatta aatcgtctga aatcgacata attttgccga acatttcgct
     2941 tggagcttca gttacaccaa tgtagttgcc gagagatttc gacatttttt tctcgccgtc
     3001 taagccgaca agtagtggga gtgtcattgc cacttgtggt ttttgaccgg ctgatttttg
     3061 taactcacgt ccaactaaca agttaaaggt ttggtctgta ccacctaatt ccacgtcagc
     3121 tttcagttct accgagtcgt gaccttgtag tagcggataa ataaattcgt ggattgcgat
     3181 cggttgttgg ttcgtgaaac ggtttttgaa atcttcacgt tctaacattc gagcaacggt
     3241 gtagttggag gctaagcgga tcataccttc cgtacctaac ttgcctaacc attcggagtt
     3301 gaatacgatc ttggtttttt gtggatctaa aattttgaag atctgctctt tataggtttc
     3361 ggcattgcgt aacacgtctt cacggctaag tggcggacgg gtagagtttt ttcctgatgg
     3421 atcgcctacc ataccggtga aatcaccgat taagaaatag acttcgtgtc cgaattgttg
     3481 gaattgacga agtttgttca gcacaacagt atgacctaag tgaatatccg gtgcagttgg
     3541 gtctgcccct aatttaatac gcaatgggcg gttttctttt aatttggtga ttaaatcttc
     3601 ttctgaataa atattttcca cgccacgttt aagttcggca aggactgatt cgatagcttg
     3661 agtcatttat cttcctttta gtcttgaata taaaaaatgg atctattata gcggtatgaa
     3721 ttgaaattga taggctatag gcgaaaaaaa acgccgtatt ttgtggggga atacggcgta
     3781 aagagttgga gtgattacct attatttttg aagtgattta atgataatga ttatcatctg
     3841 ttaagtcaat actttttttg aaaattttca ataagaatta ttctcatcaa ttaagtaaga
     3901 tggatactac ggcaattagt gaagaatgta cagcggaatt ttcgtattgt aagagtgttg
     3961 gtaagcggta tttttagaaa aaacctccga gagcggttat ttttcggagg ttttttgcaa
     4021 atgaggattt acatcgttga aatgtatttc aacatcaccc ccgctgcaat cgcagagcca
     4081 atcacgccgg caacgtttgg gcccattgcg tgcattaaca agaagttttg tttatcgtac
     4141 tcttgtccga ctttgtttga tacccgtgca gccatcggca cggcagaaac acccgctgag
     4201 ccgataagtg ggttgatttt gtgctgacta aagcggttca ttattttcgc cataatcaca
     4261 ccgcttgcag tgccgatccc aaaggcaatc acgcccaata ctaaaatgcc tagtgtttgc
     4321 ggttgtaaga atttgtctgc gaccaatttt gcccccaccg ataaccctaa gaaaatcgtc
     4381 acgatattga ttaacgcatt ttgggcggtg tcgtttaagc gttctactac gccactggtt
     4441 cgcagtaagt tgccaaagca gaacattccc aataacggtg cagcatccgg cagtaagagt
     4501 gccactaata atagcaacac gatagggaaa ataattttct cacggttgct tacattacgg
     4561 agctgaacca ttcggatttt acgttcttgc tcggtggtta aggctttcat aatcggcggc
     4621 tgaatcagtg gcactaacgc catataagaa taagccgcta ccgcaatcgc acctaataat
     4681 tcaggggcaa gtttgctggc aagataaatc gcggtcgggc catctgcccc gccgataatc
     4741 ccaatcgcag cggcttgtgg caatgtgaag tcgataatgc ctaaccagtt tagccctaat
     4801 gcacccagta cggtagcaaa aatcccaaat tgagccgcag ccccaagcat taacattcga
     4861 gggttggcaa gtaaagggcc gaaatcagtc atcgccccga cacccataaa gataataagc
     4921 ggagcaacgc cgtagccgat agcaacttta tagaataatg ccaacacacc ggcggtatag
     4981 cccatatcgc cagccattac ttcaagctca ttttgaacag agggagcagc cagtgcgatg
     5041 gcatctttaa tcgaatgcag atcaaggctt gtactattta ccttagcagc aataattgcc
     5101 agttgctcgg cagaaccgct gtggagtaaa ttgtctaatg ccgtcattgc catacctgct
     5161 tccggaatgt ttgacatcaa accaccaaag ccaataggca gtagtaaaag cggttcgaat
     5221 tttcgggcga tggcaagcca aagcaacact aaactgatga caatcataat cgcttgcccc
     5281 agttctaaat gcattagccc cataccttta aaaagtgcaa taatactttc cataccggac
     5341 tcctaggcaa tactcattaa agtatcattt accgccacaa catcgccttg tttgacatta
     5401 atgccttgaa tcgtgccgga ttttgccgca caaatttggg tttccatttt cattgcttca
     5461 agaatcagta acacatcgcc ttcagcaact tgttgcccct ctttcacttc cactttcaaa
     5521 atgttccctg ccattggagc attcaccggt tcggcattgg ctgatagcgt atttgcagat
     5581 tttggtgcag aatcgaccgc ttgcggagcc ggtgttgctg gtgttggagc gatattggtt
     5641 atatcgccac cttcactcac cttcaccaca aaggctttgc cttcaagctc aacggtataa
     5701 atggctgaac cggattgtgt ttgggctttt ggtgcagaag cagcggaggc ttgtgccggt
     5761 tttgcttcat cagcacttgg cactggctca aaggcagacg gattattgcg gttttccaaa
     5821 tatttccagc ccacttgttg gaagagtgcc acaatcaata catcatcaat catgttttca
     5881 gatagcgtaa tgcctttttc ttttgcactt gcagtcactt ctgccactaa tttttccagt
     5941 tccggcggaa gatgatcggc tgggcggtcg gtaatcggct ctgctccctc aagcactctg
     6001 tcttgcaagg ctttgtttac cggtgccggt gtgcgaccat attcgccttt taaaatacct
     6061 gcagtttctt tggcgatagt tttataacgt tcgcccatta atacgttcat caccgcttgt
     6121 gtgccgacaa tttgtgaggt tggtgtcacc aacggaatat tgcctaaatc ttcacgcaca
     6181 cgaggaattt cttgtaaaac caagtctaat ttatccgacg cattttgttg cttgagttgg
     6241 ctttcgaggt tggtcagcat gccacccggc acttgggcaa ccaaaatacg gctatctata
     6301 ccacggagct gtccttcaaa ttttgcatat tttttacgca cgttacggaa gtaggcagag
     6361 attttctcca gagctggaat gctcaagccg gtttcatacg gcgtgccttg aagtgtggcg
     6421 acaatagact cggttgccgg gtgaccgtaa gtgccggaca ttgatgaaat ggaggtatcg
     6481 acaccatcga caccggcttc aatcgctttg agcaacgcca tttcagccat accggtggtt
     6541 gaatggcaat ggagatgtag ctcaacgtcg tagcgttttt taatttcgct gactaattca
     6601 taggctgcca ttggggttaa aatgcccgac atatctttga tcaccaacga atccacgcca
     6661 atttccaata attgctcggt tacatccaac cacgtttgta gggtatgaac agggctggtg
     6721 gtgtaactca aagtaccttg tgcgtgcccg ccgtgttttt tgaccgcttg taatgcggct
     6781 tgcatattgc gagggtcgtt gagggcatca aatacacgga acacactcat accgtttgcc
     6841 accgaacgct cgacaaattt atctaccaca tcgtcggcat aatgacgata acccaataag
     6901 ttttgcccac gtagcagcat ttgcattggg gttttcggca ttgctttttt cagctcacgt
     6961 aaacgcaccc acggatcttc gcctaaaaaa cgaatacaac tgtcaaaagt tgccccgccc
     7021 caagtttcta acgaccaata gccgatatta tccagctcgg aggcaatcgg taacatatcg
     7081 tctaagcgta aacgagtcgc aaaaagggat tggtgtgcat cacgcaaaac cacatcggta
     7141 atcgcaattt ttttcggttg agttgtcata atcaaattcc ttctaattat tttatttttg
     7201 aaatcttgtt aatgttgccc gttaattcga cgatgatgag caatcgcagc ggcaatcaca
     7261 gggcgtaaac gctctaaatc atctggggga gattgaggca cagttttaga ggctggaata
     7321 gaaacaggtt cggggaaata ttgattgata atcttggaaa taaagctgat ggcataaatt
     7381 aaaaccagca ggaacagcat aacaaatccc attccggcta ccattaaatt tataccttcc
     7441 gcaaagagtt ctgcaggtgc cataatttat ccttaaaatg tagaaatgat gaagaaaatg
     7501 tagaaaaaat tcagatttat ttcaaattat ttagcctcaa aatgagtggt agatcacaaa
     7561 ctttaaacgc aagcggtcag attttacaaa ttttttacaa gagtagttaa aatttgcaaa
     7621 acctgacctt aggttagctt gaagctaagt cgttatgcca ttagccccga aatatatttt
     7681 gaaagggtca ttgcacttag caccgccatt agaatcacca caatcgcccc tgaaacggtc
     7741 atccacacag ggtgtttgta gtcgcccaca atcttggttt tgtgagctgc cagcagaatt
     7801 aacccgaggg aaatcggtag aattaagccg ttaagcgtcc ctacaaacac caagacttgt
     7861 gccggtctgc cgatggttgc cagcacggcg gttgaaacca caataaaggc gacaatgaaa
     7921 cagtttttgt gtttttcgat aaatggcgag aatgaagtaa tgaaggaaac cgaggtataa
     7981 gccgccccaa tcacagaggt aatggaggct gcccaaatca ccacgccgaa gatcatcaaa
     8041 ccgacatcgc cggcaacgtg ggcaaatggg gtagccgcag ggtttttcgg atcaagttgc
     8101 acgcccattg acaccacgcc cagcaccgct aaaaacagta ccacacgcat aattgaggcg
     8161 attaaaatgg ctgacaccga gcttttgctc acctctttta aattctcttt gcctttaatg
     8221 cctgcatcaa gcaaacggtg cgcaccagca aaggtgatgt atccaccaac agttccgccc
     8281 actagcgtga caatcgcaat cacgtctaat ttttccggca caaaggtatg caccaccgca
     8341 tcgtcgattg gcagattggc ttttacggca acgaagatgg ttaaagcaat catcacaaag
     8401 cccataattt gtgcaaatct gtccatcact ttaccagctt catggaacag gaacacgccc
     8461 acggcaatcg caccgctgac caccgcaccc atttcagggg aaagtccggt gagaatgttt
     8521 agccctaaac ctgcaccgcc cacgttgccg atattaaagg ctaaaccgcc catcacgata
     8581 agcagagcca gaaagtaacc tgcccccggc aatacggcat tggcgatgtc ttgagctttc
     8641 ttttccgata ctgctacaat tcgccaaata ttcagttgag caccgatatc aagcaagatc
     8701 gagagtaaga tcacaaagcc gaagctggca agtagggttt gggtgaaggt ggctgtttgg
     8761 gttaagaacc ccggcccgat ggcggaggtc gccattaaaa atgctgcccc gagtagggcg
     8821 ctacggttgt tgtttgacat tgtgttggtt ccttttttgt tgttaatgag tcattattcg
     8881 tggatttatc atggtaatat tcccctcttt agcaaagagg ggttagggga gatttggcag
     8941 tagaactcat cgaagtgaga gagttaataa tgctcaattt aacctcttga aattacttat
     9001 tttctccgta aatcgcttct gtcaaatctc ccccagcccc tcttttctaa agaggggagt
     9061 tcgatctcca agagttttat ttctggtttt ccgtccattt tgactaaaac aggatcaggc
     9121 tttcaccaaa atcccctttg cctgtaactc ttttttaatt cgctcggcaa ataccacggc
     9181 gtgttcgccg tcaccgtgta agcaaatgct gtcggcttta atcgccacat taccaccatc
     9241 aacggttggt acagtgccgt ctaacaccat ttgcaagact tggctgatcg cttcatcatc
     9301 gctttctacc attgcatcat cacgggtgcg aggcacgagc gagccgtcag ctaaatagtg
     9361 gcggtcggca aagacttcgg aaatggtttc caaaccgtgc tgctcggcaa tgctcaacat
     9421 caggctgccg gataagccca tcagtttgag gttgggatcg aactgtttga ccgtattggc
     9481 gataagggtg gcaaggtgag ggtctttggc ggccatattg tataaagcgc cgtgcggttt
     9541 aacgtagctc atcggcatat tttccgcatc acataaggct ttggcggctc ccagttggta
     9601 gagcagacag gctttaagtt catcatcggg cagttgcata ttggtacgcc caaagttttc
     9661 cctatctgga aaactcgggt gagcgccaac tcgcacgctg ttttccttcg cccagttaat
     9721 cgctttacgc atttcattgt aatcaccggc gtgcaagcca cagcagacat tcgccgaagt
     9781 aacgagctgt aataatgaat gatcgttgcc acaaccttcg gcaagatcga catttaaatc
     9841 aacgtgattc attcgctttt ctccgaattg tttccaaata attttgatct ttttgataaa
     9901 tttcgaccgc ttgttcacgg cttacttgct caaattgcac cgttgagccg aacggatttt
     9961 gtgccaaccg cccgagatcg gcctgaataa tgcaggcaat tttcggataa ccaccggtgg
    10021 tttgggcatc tgccatcagc acaatcggct ggccgtccgg cggcacttgc accgtaccac
    10081 tcggggctgc gtaggagagc atttcaaggg aagcggtcaa ttccaacgct ttttctgcaa
    10141 aacggtagcc catacggttg ctgttgtttt gcagttgcca cccttgttgc caaaagcgtt
    10201 cacggctatc tgcggtaaag gcttcatatt cgctcgaagc ggttgcccga atgcgtgagg
    10261 tgaaatcaat cggctcaatc gacatcaaag aaagtcggct gtctctgcgg cctgtggcga
    10321 tgttatcgcc ggcttttaac attctgcctt gaaaaccgcc aaattgagct ttgagagcgg
    10381 tacttgccga acctaacacc tgcggcacca gaaaaccgcc tgccacgcat aaataggcgt
    10441 aattgccgag aacaatccgt tttatcgtca gcgtttgacg ggcgttggca gtgtagcggt
    10501 aataggaaaa aaccggcgtg ccgtccagct ccgcctccac caatgcaccg gtgaggcaaa
    10561 atggcgtgtc gcaatcaaag gtaatccgca ttccgccaag ggtaagttca atcgcaggtg
    10621 cattatttgc attttttaac aataaattac ccgcttgtaa ggcaaggctg tccatcgcac
    10681 cgccgtggct cacgcctaaa tggcgataac cataacgccc taaatcttga atgtgggcaa
    10741 aaccctgtat tatttcgata taaatcatag cgttatactc tccgccacaa atctaacatt
    10801 atcgccggct gtaagtagcg ttggcggatt gtgttgaata tcaaacaacg gtaactcggt
    10861 tcgtcctaac agttgccagc cacctggtga ggtaaaggga taaatgcctg tttgcctgcc
    10921 gccgatgccg accgaaccgg cagggacttt ggtgcgaggc acgtttcggc gtggggtgtg
    10981 taaattttgc ggtaagccgt tgaggtaagg aaagccgggt tgaaagccga tcataaacac
    11041 ggtgtaagtc ggttcggtat gacggcggat aatctcttcg ggagtggtat tgtggaattg
    11101 ggcgacttca aacagatctt cgccaaattc accgccgtaa tggacaggga tttcaatcag
    11161 gcgtggggat tgtgcggctt gggcttggtc gttttcccag agttgttgaa ccgcttggat
    11221 aaggcgtgtg ctatcggtgc caatggcgtg aaatagggta aaattgttca ttcctaccac
    11281 cacttccaac acgccatcta aggctcttgc ctgttctgcc acccgccaaa tctgttgctg
    11341 tttggcaagc tctgcaggcg gttcggctaa gcagagcagg gaatgttcgc tggtatgatg
    11401 aatcgtcagc ataattgccc tcaaattgtt acaaaaaggt aacaactttt tacgctgata
    11461 tatccagact gtcaatttta ttttgttgaa aatgtgaaat agctcttaat tttcagaaat
    11521 ttgatcattt cttattcgcc aaaacgttgc aaatctgggc ttgcctgatt ctgtgacacc
    11581 tcgatattta taggtaatga ttgagccaat cggtggcgga ttttctcgtt ctttatcttt
    11641 aaaaccgctg ccaatccgaa aacgtccacg ttgattttca caagtgattg ccccgagttt
    11701 atcggtatat ttccctttgc ctttatgatg ggcaaccaca gtgcattcct catctaaaac
    11761 cggtttgatt ttcaaaattt gggcagaacg accttgaata tagcctgcat tcgggttgcg
    11821 aacgataacc ccttcgccac cttgttccag cacagattgg taaaattgtt gaaggtgggc
    11881 tttatcttta atcggaattt gctcaataat agcgatatgg agcgttggat gttgcttcag
    11941 ataattctct aaggtcgcca gccgttcaat caaattccct tgtgcatttg gcacatcgaa
    12001 cacctgtaat ttcagcttgt gccaaccttt gggctgagcg gaacgcacaa tgcttgaaat
    12061 ttcctcaaat ttgccccgtt cgctaaacag ttcgccgtca atcgcaaaag gagggaaatc
    12121 ttgaataaaa tagtcgggtg gattgagcgg ataaccttgg cggctgatga gctgttgacc
    12181 gtcccaaaag cctcgcacgc catcgagttt ttctgacatt acccagccac tgatgtcttg
    12241 atcttgatat tgctcaacga gcattaactc gggcgatttg gcaaaaatag ggttaccaat
    12301 gaagagtagg aataagagca gttttttcat agtctctccg aaatagtgcc tccgaatatg
    12361 caaggcacta taaaggaaag cgtgcaaaca gtcagaaatg aatctcaatt tttcgacgca
    12421 gatcgcaaaa ttttccgaaa aactgatcgc ttgagtgaga aatttaacgt actgtttgaa
    12481 ttaaagcaga aatatcctcg ctaaaggttt ccaacttcgc catttcttca atttggctat
    12541 gagaatattt cttcacattg taaacgacca ccatacttac atcacctact tgcaataaat
    12601 tactttcgcc aaaaggcgtg tagcgtgttg ccccaatgct gataatcgct ttagttgggt
    12661 agtttgcctg ttttaacatt tcggaaatgt tgttcatcgg accttcatcg ggctgattat
    12721 tcattctctc tacaatccaa tcgagcaatt tttgatggaa atagctgtac tccaccgcag
    12781 ggctgtcgat gccgtaggta ttcagctcgc cattacgttt atggaaacaa gcgatgttgt
    12841 aattatccag cgtgccgcct ttgttgaagt tatctaacgg aagatgaatt gccgaaagcc
    12901 ctttagacga agccccccag tttttctttt cacagatttt attcgcattc ggacggcgga
    12961 tagagcagtc attgtaagcc gcaaaatggg ttggcgtgag cgaaaccact tgctgatctt
    13021 ggtagccaat ttcacaaatt aacgccactt caggctcaat ttgcagatta tcagcatcac
    13081 tcgggaaatt aatcgcagta tttgaaagcg gataggtgga taaaaactga taatccccca
    13141 aattttccgc cggcacataa aacgggaaaa tcgcctttgg ctgcactgct tccactgttt
    13201 tcactttcgc aaaatccgcc tcctctccgg cttgttctaa atgtccagca aaattgccgg
    13261 ccacgcctaa accgataaag tgttccataa tctactcctt aattggtaaa agttggataa
    13321 aattttaccg cttgtaacca aaaattgcga atgtttattt tgatctgatt gatagaaaat
    13381 tattgaattg agattaaatt tagccatatt ttgaacataa aattagcgct ttgttataaa
    13441 aaca
//
LOCUS       DANLUQ010000057        13319 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.057, whole genome shotgun sequence.
ACCESSION   DANLUQ010000057 DANLUQ010000000
VERSION     DANLUQ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13319
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.057"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..263
                     /locus_tag="PXB27_001294"
     CDS             <1..263
                     /locus_tag="PXB27_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5346082.1"
                     /translation="ARKAQRRLSFLRVFSLAFAAFIVNTTEFIPIALLSDIASSFQME
                     PSSAGIMMTVYAWVVFLLSLPLMLLTANMERKRLLLMLFMGE"
     gene            260..961
                     /locus_tag="PXB27_001295"
     CDS             260..961
                     /locus_tag="PXB27_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5346083.1"
                     /translation="MIGQALGWRATFGIIGIIALIVLMMIWRLLPYLPSKNAGSIKSL
                     PLLFKRPMLIGIYLLVALIVSGHFTAYSYIEPFMIKISSVSVENTTFILLVFGLAGVV
                     ASFLFGRLYGKAPNKFILSGIAIIMCSQLCLLFLSDSTVAMFMVAFVWGIGITGMSIA
                     LQMKVLELAPDATDVATAIFSGTYNLGIGGGALLGSIAIQQLGLANIGFAGGSIAFVA
                     MVWFVFVSLKYRQLR"
     gene            complement(1028..2761)
                     /locus_tag="PXB27_001296"
     CDS             complement(1028..2761)
                     /locus_tag="PXB27_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3413 domain-containing protein"
                     /protein_id="HDL5346084.1"
                     /translation="MFARLRSLLPTNSRQYREETSQKITWGHWFALFNIVLALLISSR
                     YAFNADWPNTLVGKLYFFVSLFGHFSFVVFAGFLLILFPLSFIVKNHRTYRGLSVILA
                     TIGQTVLLVDTEVFKQFYLHLSPLVWDLLVNPEEGELTRKWQLLFVPMPLLLLAEMLY
                     SRWCWQKLRSFSRQKWGKYVALFFVISFTATHLIYAWADMNAYRPITAQRANYPLSYP
                     MTARNFLEKHGFIDRVEFNQEIEESGRLDTFYLNYPKSPLIATKTPSTNILLINLSGL
                     SKQIINAETMPKLTEISQQSHRFMQHYSSGDSASAGALGLFYSLSGKYLDSVLADKTV
                     SPLIEIARKSDYQLGLFSHNGFADPIYHQAIFSGILLPTAHSNVGAIGQWKNWISSRS
                     LENPFFSYLDLRMPFAHTAQEQAKLLDWQFAEIWQHLTQNALLANTLVIITADISNEV
                     GAENSFDKQHIQVPMMFYWQGESKEYHFLSSHLDIMPTLLSQFFGVQNPLNDYAQGMD
                     LSTQNQRLWVSSSNHKWNVAIMPNGDQYQIDRKGNFKHFNSNGEHEKDARPPLALFLQ
                     MIQQSNQFIEK"
     gene            complement(2766..2987)
                     /locus_tag="PXB27_001297"
     CDS             complement(2766..2987)
                     /locus_tag="PXB27_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1414 domain-containing protein"
                     /protein_id="HDL5346085.1"
                     /translation="MATKSKYHTKQFEQLLADLIATIEKHKAPVDLSLMALGNMITNI
                     LIENIQSPAQREALADAFADALKQSLKTK"
     gene            3226..3798
                     /locus_tag="PXB27_001298"
     CDS             3226..3798
                     /locus_tag="PXB27_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL5346086.1"
                     /translation="MSRRSEPKEEMVNRILSATESLIVQEGLQNLSMRSIAKEAGIAS
                     GTLYLYFKTKDDLLHSLAAQLLECYNGYMHFEFKPEMALFEQYKALVNKKWQFLIERL
                     DLVKQFQAVIGIDELIRETIADETSFWNKLILEGKKQRIIADLSNELLYGLSIGTLVD
                     IQYLQNLNQENQFDGYLDEIILRTWKAITF"
     gene            3829..5034
                     /locus_tag="PXB27_001299"
     CDS             3829..5034
                     /locus_tag="PXB27_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HDL5346087.1"
                     /translation="MTTETQKLGKGRKFLIVLTLLIVLIVFAAIVGLQKIGAMKKAEA
                     AANMPEAVSEVTAMQVTTQEWTPTISAVGYIRPNQGAMLSAETSGVVSRVLVTSGQRV
                     KKGDLLVEFDSSVEIANLRASEAQLPNAKANLERYRNLIASNSASKAEFDNAQSTYNQ
                     LLANIEALRATIKRRQIYAPFSGLAGIVNVNVGQYITMGTEIVRVEDQSSMKVRFTLP
                     QTNLEQISIGQKVTAVIDALPAQTFPAKIVAIDPAVDRLTGLINVEAVIEEGQEKLLS
                     GMFARLNVALPTETGQIVVPQISIAYTMYGETVYVLQPLSDEDKEMVNKMAVQNPSLD
                     VNKMYRAKQMEVKTADRKGNYSQLSKGVKAGDLIVTGGLQRLSNNALVKVSDTEAVGI
                     TTPAKNSKL"
     gene            5048..8215
                     /locus_tag="PXB27_001300"
     CDS             5048..8215
                     /locus_tag="PXB27_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HDL5346088.1"
                     /translation="MKFTDIFIKRPVLAVCISLLITILGLQAIEKLQVREYPEMTVSI
                     VTVSVNYSGADARLMQTLVTSQLEEAVAQADNIDYMTSSSSPSTTTVTAKMKLNTDPN
                     AALSDISAKVNAVRSNLPSGIDDPSIRVSTGSNDALMYIRFVSDELNTSQVTDYINRV
                     VKPQFFTVNGVSSVDVYGSVFGLRIWLDPDKMASNNLSGAAVLSALSANNLQTAAGNS
                     NGYYTVYRNKVLSTTPSVTELEEVTVSTTKDGKTIKLKDIATVELDKYQDAARATVGG
                     KDAVVLAVSVAATANSLTVAKDIYPMFEQVLKNLPSTIQGDIVYDKTIAIDSSIKEVI
                     KTIGEATVIVLVVIILFLGSLRAMIVPVITIPISLIGVLFFLQMFGFSINLLTLLALV
                     LAIGLVVDDAIVVLENVERHVKEGKTPFDAAIIGTREIALPVISMTITLGAVYSPMAL
                     MTGVTGTLFKEFALTLAGAVFISGIAALTLSPMMSSRILKEHKEGEESAFARVVHRSL
                     DKMTSCYTNMLSGIMAVRGFVLFFAVLIFASLPMLFGSLSSEVAPTEDRGFVVGISNG
                     PSNTNLDYTEAALAQFNQEVTKLPEVSSLMTVSGFNGANSALSIIALKDWNDRSRERA
                     AISNDVAAIGKKVVGMDVNAIAFPEISTGENDLPFSLVITTADSYEKLAIVASEFLKK
                     AQTSGQFFFANLDLKFDTVTMNMKFDREKMGAYGVTMQQVSGTLGAFLSGAIITRVDI
                     DSRAYPIVSQAVRNDRLNPEDLMNYYVTTATGQSIPLGSFVTMELKTSPSSLPRMSQL
                     NSATIGGVVSGSIGDAVNWAKAELDRTLPSGYQYDFRGESRQFVQEGNAMAMTFALAV
                     IIIYLVLAIQFESWRDPLVILVSVPLAVSGALLVMNIPTAMKGLFMGIAAGTGSKDMM
                     AFANGIWTAGYTLNIYSQVGLITLVGLITKHGILMCEVAKEEQLLHGKSRYEAIFHAA
                     TIRLRPIMMTTAAMIAGLIPLLFAVGAGAIARFSMGMVIVAGLAIGTLFTLFILPVIY
                     TFLGQEHKPLREFDEEKYFPTTKEA"
     gene            8443..9906
                     /gene="guaB"
                     /locus_tag="PXB27_001301"
     CDS             8443..9906
                     /gene="guaB"
                     /locus_tag="PXB27_001301"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005725353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDL5346089.1"
                     /translation="MLRIKQEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTETKLAISLAQEGGIGFIHKNMSIERQADRVRKVKKFESGIVSEPVTISP
                     DMTLAELAELVKKNGFAGYPVIDENQNLVGIITGRDTRFVTDLSKTVREFMTPKDRLV
                     TVKENASREEIFHLMHEHRVEKVLVVNNEFQLKGMITLKDYQKAESKPNACKDEFGRL
                     RVGAAVGAGPGNEERIDALVKAGVDVLLIDSSHGHSEGVLQRVRETRAKYPDLPIVAG
                     NIATAEGAIALADAGASAVKVGIGPGSICTTRIVTGVGVPQITAIAEAAAALKERGIP
                     VIADGGIRYSGDISKAIAAGASCVMVGSMFAGTEEAPGEIELYQGRAFKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRIAYKGYLKEIIHQQMGGLRSCMGLTGCATI
                     EELRTKAEFVRISGAGIKESHVHDVTITKEAPNYRMG"
     gene            10027..10698
                     /locus_tag="PXB27_001302"
     CDS             10027..10698
                     /locus_tag="PXB27_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL5346090.1"
                     /translation="MLQAIIFDMDGVIVDTEYLEFSLQKQFIEDIKEHSRPITLEQQS
                     EVVGKCLKEIPVIIKKLSESSLPIEEIRARYYAFFQDLFSKVDFKTIFRADIQQIIQF
                     AKQNQIKLAVASSSALSHIENILTVCGIKDEFDLIVSGEQFEHSKPDPTIYRYTCEKL
                     GVEPQNAVAIEDSYYGMLAAKTAGLTVIGYEEKRILIDQSLADYMGKDMKEILKILQK
                     CNTPS"
     gene            10820..11131
                     /locus_tag="PXB27_001303"
     CDS             10820..11131
                     /locus_tag="PXB27_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346091.1"
                     /translation="MKKLSLISIVALSACSIFGPSYSGETTASPLLKLDTERNINLYF
                     QAIHKCTPDKIHTKIINIPSEGNAKELWTATGCNKTQSFIITYRNDGVGGTFINMTTT
                     P"
     gene            11148..12719
                     /gene="guaA"
                     /locus_tag="PXB27_001304"
     CDS             11148..12719
                     /gene="guaA"
                     /locus_tag="PXB27_001304"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012510808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDL5346092.1"
                     /translation="MTNIHHHKILILDFGSQYTQLIARRVREIGVYCELWAWDVTEEQ
                     IREFNPTGIILSGGPESTTEENSPRAPEYVFNAGLPVLGICYGMQTMAMQLGGLTETS
                     DHREFGYASVALKSADQLFANLNDDLTACEPKLDVWMSHGDKVTKLPPHFTITGVTPT
                     CPIAAMSDESRRFYGVQFHPEVTHTKQGLALLKNFVVGICGCETNWTAENIIEDAVKR
                     IREQVGDDEVILGLSGGVDSSVTALLLHRAIGKNLHCVFVDNGLLRLNEGDQVMEMFG
                     NKFGLNIVRVNAEDRFLEALKGLDEPEAKRKTIGKVFVDVFDDESKKLSSVKWLAQGT
                     IYPDVIESAASKTGKAHVIKSHHNVGGLPDYMKLGLVEPLRELFKDEVRKIGLALGLP
                     AEMLNRHPFPGPGLGVRVLGEIKKEYCDLLRKADAIFIEELHKAGWYYKVSQAFTVFL
                     PVKSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDLLGKISNRIINEVNGISRV
                     VYDVSGKPPATIEWE"
     gene            complement(12880..13275)
                     /locus_tag="PXB27_001305"
     CDS             complement(12880..13275)
                     /locus_tag="PXB27_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcB family protein"
                     /protein_id="HDL5346093.1"
                     /translation="MEQWTTDVWAAIMAAFIVGAVIGCTLVRVFNSSVRKQHELEKEL
                     KQTKTQVDEQKQKLEQHFEQSAQLLSSLASDYKKLYTHLAQSSEKLLPEASKNITFFQ
                     QTKLPIPAEKTEEDQPRDYSEGSSGLLKS"
BASE COUNT         3860 a         2539 c         3028 g         3892 t
ORIGIN      
        1 ttgctcgaaa agctcaacgg agattatcgt tccttagagt atttagtctt gcctttgcgg
       61 cttttatagt aaacaccacc gaatttattc ctattgcatt gctttcagac attgcaagca
      121 gtttccaaat ggagccttca tcggcaggca ttatgatgac ggtttatgcg tgggtggtat
      181 ttttattatc gttaccgttg atgttactca cggcgaatat ggaaagaaaa cggctgttgc
      241 tgatgttatt tatgggcgaa tgatcgggca agccttaggc tggcgggcaa cctttggcat
      301 tattgggatt atcgctttga ttgtgctaat gatgatttgg cggttgctgc cttatttacc
      361 aagcaaaaat gcaggctcga taaaaagttt gcctttgtta tttaaacgcc cgatgttaat
      421 cggcatttat ttgctggtgg cattgatcgt ttcaggacat tttaccgctt acagctatat
      481 tgagccgttt atgattaaaa tcagttcggt gagtgtggaa aacaccacct ttattttgct
      541 ggtttttggc ttggcaggtg tggtggcaag ttttttattt ggtcggttat atggcaaagc
      601 cccgaataaa tttatcttaa gcgggatcgc cattattatg tgcagccagc tttgtttact
      661 gtttttgagt gactcaacag tggcaatgtt tatggtcgct tttgtctggg gaattggcat
      721 taccggaatg agcattgcgt tgcaaatgaa ggtgcttgaa cttgcccctg atgcgactga
      781 tgtcgcaact gcaatttttt ccggcactta taatttaggc attggcggtg gagcattact
      841 ggggagcatc gctattcagc aactgggatt agccaatatc ggatttgccg gtgggagtat
      901 tgcttttgta gcaatggttt ggtttgtatt tgttagcttg aaatatagac agctcagata
      961 gtgacaagcg gtcatatttt gctgaaattt tgcaaatttt cctgcaaaaa tgaccgcttg
     1021 ttgaacatta cttttcaata aattgattgc tttgttgaat catttgtaag aacaacgcta
     1081 atggtggtcg agcatctttc tcgtgttcgc cattgctgtt aaagtgctta aagttgcctt
     1141 tacgatcaat ttgatattga tcgccattcg gcataatcgc cacattccat ttgtgattag
     1201 aactggatac ccataaacgt tggttctggg tgcttaaatc cataccttgt gcataatcat
     1261 tcagcggatt ttgcacgccg aagaattggc tcagtaaggt tggcataata tctaaatggc
     1321 ttgaaaggaa atggtactct ttgctttcgc cttgccagta gaacatcatc ggcacttgaa
     1381 tgtgttgttt atcgaagcta ttttctgctc caacttcatt actaatatcg gcggtaatga
     1441 ttaccagcgt attcgccaat agtgcatttt gggtcaaatg ttgccagatt tcagcgaatt
     1501 gccaatctaa taattttgcc tgctcttgag cggtgtgggc gaagggcatt cttaagtcta
     1561 aatagctaaa gaacggattt tccaatgagc gagagctgat ccagtttttc cattgcccaa
     1621 tcgcccctac attactgtgc gccgtaggca ataaaatacc agagaagatc gcctgatgat
     1681 aaatcgggtc ggcaaaacca ttatgtgaaa acagccctaa ttgatagtcc gattttctag
     1741 cgatttcgat aagcggtgac acggttttat cggctaaaac ggaatccaaa tatttaccgc
     1801 ttaaactgta aaatagacct aatgcaccgg cagaggctga atcaccacta ctgtaatgct
     1861 gcataaagcg gtggctttgt tggctgattt ctgtcagctt aggcattgtt tcggcattta
     1921 ttatctgttt agataaacct gataaattaa tcagtaaaat attagttgag ggcgttttgg
     1981 tcgcaatcaa gggagatttt ggataattca gatagaaagt atctagtctg ccactttctt
     2041 caatttcttg attaaattct actcggtcaa taaaaccgtg tttttctaag aaattacgag
     2101 ccgtcatcgg gtaagaaagt gggtagtttg ctcgttgagc ggtaatcggg cggtaggcat
     2161 tcatatctgc ccacgcatag attaaatgag tagcggtaaa gctgatcacg aaaaagagag
     2221 cgacatattt gccccatttt tggcggctaa aactacgcag tttctgccag caccagcgag
     2281 agtagagcat ttctgccagc aaaaggagtg gcattggtac aaacagcagt tgccatttac
     2341 gggtcagttc cccttcttcc ggattaacta acagatccca tacaagcggt gataaatgca
     2401 gataaaattg cttgaagact tcagtatcca ccaataacac ggtttgcccg atggtggcta
     2461 aaatcacgga aagcccacga taggtgcgat gattcttaac aataaaactg agcggaaaca
     2521 gaattaatag aaatcctgca aagaccacaa agctgaaatg cccgaacagg ctgacaaaga
     2581 aataaagttt gcctacaagc gtgttcggcc agtctgcatt aaatgcgtaa cgagaggaaa
     2641 tgagtagtgc caacacaata ttaaatagtg caaaccaatg cccccacgtg attttctgtg
     2701 aggtttcttc tcgatattgg cgagaattgg tcggcaataa actgcgtagg cgtgcaaaca
     2761 tcatcttatt tggtttttag tgattgtttt aatgcatcgg caaaggcatc agcgagagct
     2821 tctctttgtg ccgggctttg aatattttca attaaaatgt tagttatcat attgcctaat
     2881 gccattaacg ataaatccac cggtgcttta tgcttttcta tggtggcgat gaggtcagcc
     2941 aatagctgct caaattgttt ggtgtggtat ttagatttcg ttgccatatt gaaataaaga
     3001 tgatcctata attaggtaca aatagtagca caaaaataat aataaagtag cgttaagcgt
     3061 tattgtttga gtgatttttg caaagtttta gccaaaaatg accgcttgtt caataaatga
     3121 ggaattttgc aactatttta ttttaatttg ataggtgttt tctgctttct tttttcataa
     3181 aaaaagctat aatgaacggc tgttcattat aggtaaggag ataaaatgtc taggcgaagc
     3241 gagccaaaag aagagatggt aaaccgtatt ttatcggcaa ccgagtcgct gattgtgcaa
     3301 gaaggtttgc aaaacctttc aatgcgtagt attgcaaaag aagcagggat cgcttccggt
     3361 acattatatc tttactttaa aaccaaagat gatttattgc actcattggc tgcccaatta
     3421 ctcgaatgtt ataacggtta tatgcatttt gagtttaagc ctgaaatggc gttatttgag
     3481 caatataaag cgttggtcaa taaaaaatgg cagtttttaa tcgagcgttt agatttggta
     3541 aaacagtttc aagcggtgat agggattgat gaattgattc gggaaaccat agctgatgaa
     3601 acttcctttt ggaataaatt aattttagaa gggaaaaagc aaaggattat tgctgatcta
     3661 tctaatgagt tgctttatgg attaagcatc ggcacattgg tggatatcca atatctgcaa
     3721 aatctgaatc aagaaaatca gtttgatgga tatttagacg agattatttt acgcacttgg
     3781 aaagcaatca ctttttaagt ttctttttat tagttttatg gagaatgtat gacaacggaa
     3841 acacagaagc tgggtaaagg cagaaagttt ttaattgtac tcacattgtt aatcgtattg
     3901 attgtgtttg ctgcaatcgt agggttacaa aaaatcgggg caatgaaaaa agccgaagca
     3961 gcggcaaata tgccggaggc ggtgagcgaa gttactgcta tgcaagtgac gacccaagaa
     4021 tggacaccaa ccatttctgc ggtcggttat attcgcccaa atcaaggggc aatgttaagt
     4081 gctgaaactt caggcgtggt tagccgtgtt ttggtcactt caggacagcg tgtgaaaaaa
     4141 ggtgatctgt tagtagaatt tgacagcagt gttgaaattg ctaacttgag agcctctgaa
     4201 gcgcaattac cgaatgccaa agcaaattta gagcgttacc gtaatctgat tgcctcaaac
     4261 agtgcttcta aagccgagtt tgataatgct caatctacct acaaccaatt attagccaat
     4321 atcgaggcat tgcgtgctac gattaaacgc cgtcaaattt atgctccgtt tagtggttta
     4381 gcgggtatcg tgaatgtgaa tgttgggcaa tacattacaa tgggtacgga aattgtgcgt
     4441 gtggaagatc aatcttcaat gaaagtacgt tttaccttgc cgcaaactaa tttagagcaa
     4501 atctcgattg ggcaaaaagt gacagccgta attgatgcgt taccggcaca aaccttccca
     4561 gctaaaattg tggctattga tccggctgtg gatcgtttaa ccggcttaat taatgtggaa
     4621 gcggtgattg aagaagggca ggaaaaattg ctttcaggta tgtttgcccg cttaaatgtt
     4681 gctttaccaa cagaaaccgg acaaatcgtc gtaccacaaa tttctatcgc ttacactatg
     4741 tatggtgaaa cggtatatgt gctgcaacca ttatctgatg aagataaaga aatggtgaac
     4801 aaaatggcgg tacaaaaccc aagtttagat gtaaacaaaa tgtaccgtgc aaaacagatg
     4861 gaagtgaaaa cggctgaccg caaaggaaac tattcacaat tatccaaagg cgtgaaagca
     4921 ggtgatctga ttgtaacagg cggtttacaa cgtttaagta ataatgcgtt ggttaaagtt
     4981 tctgatacag aagccgtagg cattaccaca ccggcgaaaa acagtaaact gtagtggagt
     5041 gagttaagtg aaatttactg acatctttat taaacgtcct gttttggcag tttgtatcag
     5101 cctattgatt acgatcctag gcttgcaagc aatcgaaaaa ttgcaggtgc gtgaatatcc
     5161 ggagatgacg gtctcgattg taaccgtgag tgttaactat tcaggtgcag atgcacgttt
     5221 aatgcagaca ctggttacct cacagttaga agaggctgtt gctcaagcgg ataatattga
     5281 ctatatgact tcatcaagct ctccaagtac cactacggta acggcaaaaa tgaagttaaa
     5341 taccgatccg aatgccgcac tttccgatat ttcggcaaaa gtgaatgcgg tacgctcaaa
     5401 tttaccgagc ggtattgatg atccgtcaat tagagtctca accggttcaa atgatgcgtt
     5461 aatgtatatc cgatttgtct ctgatgaact taatacatca caagttaccg attatattaa
     5521 ccgtgtagta aaaccacagt tctttacggt aaatggtgtt tctagcgttg atgtgtatgg
     5581 ctctgttttt ggcttacgca tctggcttga tccggataaa atggcgagca ataacctatc
     5641 aggggcggct gtattatcgg cattaagtgc caataacttg caaaccgctg cgggtaattc
     5701 taacggttac tatacggttt atagaaacaa agtactttct accacgcctt ctgtgacaga
     5761 gttagaagag gttacggttt caaccaccaa agatggaaaa accatcaaat taaaagatat
     5821 tgcgactgtt gagctggata aataccaaga tgcagcaaga gcaacggttg gcggtaaaga
     5881 tgcagttgtg cttgctgttt cggtagccgc aacggcaaac tctttaaccg tagcgaaaga
     5941 tatttatccg atgtttgagc aggtattaaa aaatctacca agcaccattc aaggagatat
     6001 tgtttacgat aaaaccattg cgattgatag ttctatcaaa gaagtaatta aaactattgg
     6061 tgaggcaacc gtcattgtat tggtagtaat tatcctgttc ttaggttcat tacgagcaat
     6121 gattgtaccg gtgattacca ttccgatttc cttaatcggg gtattattct tcctgcaaat
     6181 gtttggtttc tcgatcaact tgcttacttt gctggcgttg gtattagcga tcggcttggt
     6241 agtggacgat gcgattgtcg tacttgaaaa cgtagaacgt cacgttaaag aaggtaaaac
     6301 gccatttgat gcagcgatta tcggtacacg tgaaattgct ttaccggtta tttcaatgac
     6361 tattactctg ggtgcagtat attcaccaat ggcattaatg accggggtaa cagggacact
     6421 atttaaagag ttcgctttaa cgcttgccgg tgcggtattt atttcgggga ttgcggcatt
     6481 aaccctttcg ccgatgatgt ctagccgaat attaaaagaa cataaagaag gggaagagtc
     6541 agcatttgcc cgcgtggtgc atagatcact agataaaatg accagctgct ataccaatat
     6601 gctaagcggt attatggctg ttcgtggttt cgtcttattc tttgcggtgc tgatcttcgc
     6661 cagtttaccg atgttatttg gctcactttc cagtgaagtt gcaccgacag aagaccgtgg
     6721 ctttgtggtt ggtatttcta atggaccttc aaacaccaat ttagattata ccgaagcagc
     6781 gttagcccaa tttaatcagg aagtaactaa attacctgaa gtatcttcct taatgacagt
     6841 ttcaggcttt aatggtgcaa atagtgcatt atcgattatt gcattaaaag attggaatga
     6901 tcgtagccgt gaacgtgctg cgatttctaa tgatgtggct gcaatcggta aaaaagtggt
     6961 gggtatggac gtgaatgcaa ttgcattccc tgaaatttca accggtgaaa atgatctacc
     7021 attctcatta gtgattacta ccgctgatag ctatgaaaaa ttggcaatcg ttgcctctga
     7081 attcttgaaa aaagcccaaa cttccggtca gttcttcttt gcgaatttag atttgaaatt
     7141 tgatacggtc acgatgaata tgaaattcga ccgtgaaaaa atgggtgctt atggcgtgac
     7201 aatgcagcag gtgagtggca cattaggggc attcttatcc ggtgcgatta ttactcgtgt
     7261 cgatattgac tctcgtgctt acccgattgt gtcgcaagcg gtgcgtaatg atcgtttaaa
     7321 tcctgaagat ttaatgaatt actatgtcac gactgcaaca gggcaatcta tcccgttagg
     7381 ttcatttgtg acgatggaat taaaaacatc accatcttct ttacctcgta tgagccaatt
     7441 aaactcggcc accattggtg gggtagtatc tggctcaatc ggcgatgcgg taaattgggc
     7501 gaaagcagaa ttagatcgca ctttaccatc aggttatcaa tatgacttcc gtggtgaatc
     7561 tcgccagttc gttcaagaag gtaatgcgat ggcaatgact ttcgctctag cagttatcat
     7621 tatctatctt gtacttgcaa ttcaatttga atcttggcgt gacccattag taattttggt
     7681 ttctgtgccg ttagctgtga gtggtgcatt gttggtaatg aacattccaa ccgcaatgaa
     7741 aggcttgttt atggggattg cagcaggcac tggctcgaaa gatatgatgg cattcgccaa
     7801 cggtatttgg acggcaggtt ataccttaaa tatctattca caagtaggtc taattaccct
     7861 tgtcggctta attactaaac acggtatctt gatgtgtgaa gtggcgaaag aagagcagtt
     7921 actgcacggc aaatcacgct atgaagctat tttccacgca gccacgattc gtttacgccc
     7981 aattatgatg acaacggcag caatgattgc aggcttaatt ccgcttctgt ttgcggtggg
     8041 tgccggtgca atcgcccgct tcagtatggg tatggtaatc gtagcaggct tggcaatcgg
     8101 gacattgttt actctgttta tcttgccggt tatctacact ttcttagggc aagaacacaa
     8161 gccattacgt gaatttgacg aggaaaaata tttcccaacg acaaaagaag catagtttta
     8221 aatagcaaaa gagcgtagcg atacgctctt ttttgttggc ttacaagcgg tcgtttttta
     8281 tttattgttt gctaaaaaat cacaaaattt aaccgcttga aagtatattt tcagatagcg
     8341 taaacgtttc cctatttaca gaaacctagt tcctctgtat aatccgctcg caattttcca
     8401 gtacagattt tcaatttaat cattttttaa ggaataattg caatgctacg aattaaacaa
     8461 gaagccctca cttttgatga tgttcttctc gtcccggcac attctactgt gcttcctaat
     8521 actgctgatc tttctactca attaactaaa accattcgtt taaacattcc gatgctttct
     8581 gctgcaatgg ataccgttac agaaactaag cttgcgatct cccttgctca agaaggcggc
     8641 attggtttta tccataaaaa tatgtcgatt gaacgccagg cagaccgtgt gcgtaaagtg
     8701 aaaaaatttg aaagtggtat tgtttctgag ccagtgacga tttctcctga tatgacatta
     8761 gcggaattgg ctgaattggt gaaaaagaac ggttttgcag gctatccggt gattgatgaa
     8821 aaccaaaatt tagtgggaat tattaccgga cgtgataccc gatttgtcac ggatttaagc
     8881 aaaacagtgc gtgaatttat gacaccaaaa gaccgtttag tgacggtaaa agaaaacgca
     8941 agccgtgaag aaattttcca cttaatgcac gaacaccgag tggagaaagt gctggtagtg
     9001 aataatgaat ttcagttaaa aggaatgatt accctaaaag actaccaaaa agcggaaagc
     9061 aaaccgaatg cctgtaaaga tgagtttggg cgtttgcgtg tgggggcggc agtgggagcc
     9121 ggtccgggca atgaagaacg aattgatgct ttagtaaaag cgggtgtcga tgtgctatta
     9181 atcgactctt cgcacgggca ttctgaaggt gtattacaac gtgtgcgtga aacccgtgca
     9241 aaataccctg atttaccgat tgttgccggt aatattgcca ctgcagaagg agcgattgcg
     9301 ttagctgatg caggagccag tgctgtgaaa gtaggaatcg gcccgggttc aatttgtacc
     9361 accagaattg taacaggcgt tggcgtgcca caaatcacgg caatcgcaga agcggcagct
     9421 gcgcttaaag aacgaggcat tcctgtgatt gctgatggtg gaattcgtta ttcaggcgat
     9481 atttcaaaag ctattgccgc cggtgcaagt tgcgtaatgg tcggttcgat gtttgccggc
     9541 acagaagaag ccccgggtga aattgagctt tatcaaggca gagcattcaa atcctaccgt
     9601 ggaatgggat cattaggtgc aatgagtaaa ggctcgtcag atcgctattt ccaatctgat
     9661 aatgccgccg acaagctcgt accggaaggg attgaagggc gtatcgctta caaaggctac
     9721 ttgaaagaaa ttatccacca acaaatgggc ggcttacgct cctgtatggg attaaccggc
     9781 tgtgccacta ttgaagaact ccgcaccaaa gcagaatttg tccgcattag tggtgctggt
     9841 attaaagaaa gccacgtcca cgatgtgaca attaccaaag aagcaccgaa ctaccgaatg
     9901 ggttaatagc ctttctcact ctctcccctt gtgggagaga gaccgtttta aattacgttc
     9961 agtaatttaa aacagagaga gggaataagt ggttagattt tcttaaaatt ttacaaaaga
    10021 ggctatatgc tccaagctat catttttgat atggacggcg tgattgtgga taccgaatat
    10081 ctggaatttt ccttacaaaa acagtttatt gaagacatca aagaacacag ccgcccgatt
    10141 actttagaac aacaatcgga agtggtaggc aaatgtttaa aagaaattcc ggtgattatc
    10201 aaaaaactaa gcgaatccag tttgccgatt gaggaaattc gtgcacgtta ctacgccttt
    10261 ttccaagatc tgtttagcaa agtggatttt aaaaccattt tcagagccga tattcagcaa
    10321 attattcaat ttgccaagca gaatcaaatt aagcttgcgg ttgcctcttc ttccgccctt
    10381 tcgcatattg aaaatattct cacggtttgc ggcattaaag acgaatttga tttaatcgtc
    10441 agcggtgaac agtttgaaca cagcaagcca gacccaacca tttaccgcta cacctgcgaa
    10501 aaattaggcg ttgagccaca aaatgcagtg gcgattgaag actcctacta cggaatgctc
    10561 gccgccaaaa ccgcaggctt aacggtgatt ggttacgaag aaaaacgaat actgattgac
    10621 caaagtttag cggattatat gggcaaagat atgaaagaga ttttgaagat tcttcaaaaa
    10681 tgtaatactc caagttaata aacggtcgat ttttatagaa attttgtcaa tgtttctact
    10741 tcaagtactt ggaacagtct atcggcttga gtaacctaga aatgagagtg gatattttaa
    10801 tattaatagg ataaatagaa tgaaaaaatt aagtttaatt tcaatcgtcg ctttatctgc
    10861 gtgcagtatt ttcggaccat cttattcagg agaaacaacg gccagtcctt tattaaagtt
    10921 ggacacagaa agaaatataa atctgtattt tcaagcaata cataaatgta ctcctgataa
    10981 aattcataca aaaatcatca atattccaag tgaaggtaat gcaaaagaac tttggactgc
    11041 aacaggttgt aataaaacgc aatcttttat tattacatat agaaacgatg gggtaggcgg
    11101 cacttttatt aacatgacca caacgccata atttaaagga aattacaatg acaaacatcc
    11161 accaccacaa aattttaatt ttagacttcg gttcacaata tactcagctt atcgcacgcc
    11221 gtgtgcggga gattggggtt tactgcgagc tttgggcttg ggacgtaacg gaagagcaga
    11281 tccgtgaatt taacccaacg gggattattc tttcaggagg tcctgaaagc accactgagg
    11341 aaaacagccc acgagcgccg gagtatgtgt ttaacgctgg tctgccagtg ctgggcattt
    11401 gctacggaat gcagacaatg gcgatgcagt tgggcggttt aaccgaaacg tccgaccacc
    11461 gtgaatttgg ctatgcctcg gtggctctca aatctgccga tcaacttttt gcaaatttaa
    11521 acgatgattt gaccgcttgc gagccaaaat tggacgtgtg gatgagtcac ggcgacaaag
    11581 tcaccaaatt accaccgcac tttaccatca caggcgttac cccaacctgc ccgattgccg
    11641 cgatgtcgga cgaaagccgc cgtttctatg gcgtgcagtt ccaccccgaa gtgacccaca
    11701 ccaaacaagg cttggcgttg ctgaaaaact tcgtggtcgg catttgcggc tgtgaaacga
    11761 actggacggc agaaaacatc attgaagatg cggtgaaacg cattcgtgag caagtgggcg
    11821 atgacgaggt gattttaggt ttgtcgggcg gggtggattc ctccgtcacc gccttgctat
    11881 tacatcgtgc tatcggtaaa aacctgcact gcgtatttgt ggataacggc ttgctccgct
    11941 tgaacgaagg cgatcaagtc atggaaatgt tcggcaacaa attcggtttg aacattgtgc
    12001 gagtgaatgc cgaagaccgt ttcttagaag cgttaaaagg cttagacgaa ccggaagcta
    12061 aacgcaaaac cattggtaaa gtgtttgtgg acgtgtttga tgacgaaagc aaaaaactta
    12121 gctcggtcaa atggcttgca caaggcacga tctaccctga tgtgattgaa tccgccgcca
    12181 gcaaaaccgg caaagcccac gtgattaaat cacaccataa cgttggcggc ttgccggatt
    12241 atatgaagct cggcttagtc gaaccgttgc gtgaattgtt taaagacgaa gtgcgcaaaa
    12301 tcggcttggc actcggctta ccggctgaaa tgctcaaccg ccacccgttc cccggcccgg
    12361 gcttaggcgt gcgtgtgctg ggcgaaatca aaaaagagta ctgcgattta ctgcgtaaag
    12421 ccgatgcgat cttcatcgaa gaactgcaca aagccggctg gtactacaaa gtcagccaag
    12481 ctttcacggt attcttgccg gtgaaatcgg tgggcgtaat gggcgatgga cgcaaatatg
    12541 actgggtcgt cagcctgcgt gcggtggaaa ccatcgactt tatgaccgct cactgggcgc
    12601 acttgccgta tgatctatta ggcaaaatct ccaaccgcat tattaacgaa gtgaacggta
    12661 tttcccgtgt ggtgtatgac gtgagcggaa aaccaccggc aaccattgag tgggaataac
    12721 tcttacaaaa taggcttctt ataacctaag ttcagtatct aaagctgaaa aatgttgtta
    12781 actgcttaat gtagcggttt agaataaagg attgattcca acggaatcaa tccttaaact
    12841 aagtgccttt tagtaaaact aaatattcaa tttttagttt tatgatttca gtaagccaga
    12901 tgaaccttcg gaataatctc taggttgatc ttcttccgtt ttttctgccg gaataggtaa
    12961 tttggtttgt tggaaaaagg taatattttt tgatgcttct ggtaatagct tttcagagct
    13021 ttgtgctaaa tgggtataaa gctttttata atcagaagct agacttgaaa gtaattgggc
    13081 tgattgctca aagtgttgtt caagtttttg tttttgctca tccacttgag ttttggtttg
    13141 cttcaactct ttttccagtt catgctgttt tctgacacta ctgttaaata ctctaactag
    13201 agtgcaacct attacagcac ctacaataaa tgctgccata atggctgccc aaacatctgt
    13261 tgtccattgt tccatcataa actccttatg ttttagtgat tgattactgc tatttttaa
//
LOCUS       DANLUQ010000058        13317 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.058, whole genome shotgun sequence.
ACCESSION   DANLUQ010000058 DANLUQ010000000
VERSION     DANLUQ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13317
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.058"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            110..3268
                     /locus_tag="PXB27_001306"
     CDS             110..3268
                     /locus_tag="PXB27_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HDL5346094.1"
                     /translation="MITINENTIEQSAIATLQSLGWDYTYGKKILAGLEHEWRERTAE
                     VILKPLLAQAIAKFNPNLPACEVENVVAQVCRADSGDLAERNRQAYDWLRNGVKITYQ
                     LYGEQVSDVVQLIDFQRPENNDFRIVNQLDISGKKGKRIPDLIGFVNGLPLVVFELKN
                     PLKENADIGKAFAQLQTYKDEISDLFVFNQALVISDGIVARIGSLTADFDRFTPWRVV
                     DEKNQSKRIVFEDELTALLQGVMTPKNLLDYVQNFVVFERDGKNRLIKKIGAYHQFYG
                     VNEAVDCTLLAATGNRKIGVFWHTQGSGKSLSMLFYAGKVLSQSSLKNPTLVLVTDRN
                     DLDGQLYATFCGGEALLKQTPIQADGRDELRSALASRSAGGVIFTTIQKFGLMEGELA
                     HPVLNERENIIVITDEAHRSQYGFSQKINHKGEYREGYAKHLRSALPNASFIGFTGTP
                     IELDDKDTQEVFGKYVSIYDFEDAVEDGATVPIIYEPRQISLGESGEFSKVMEEAQQL
                     IDDDENSYNFRLREKLHSVDSRLQKMAEDIIAHYDERTKQQDGKAMVVVMSRAICVKL
                     YEKITALRPEWHSNDVLQGSIKIVMTSNASDPAEWQKHNQDKKTLEKRFKDPDDPLNI
                     VIVRDMWLTGFDAPCCNTMYIDKPMSGHNLMQAIARVNRVFRNKSRENGGLIVDYVGL
                     TDELEKAMKQYTNAGGKEKPVRDISAVLEKMVEHITVIRGQFATPIDGQAVDIAKMLQ
                     ISEPPKLLNAILTSANHILALDRIQPPDNTAKDKTPRKNAFLQSVRLAKKGYALCGAL
                     KAVEPYKQELAFYDAVRATIIKNSTAPRNSSSENDRLLQLTALMNRAVQSDGVVDLFD
                     LLKKDRPNINLLSDEFLETVKNSPTKDLWLSAMERYLASQLREQSGANLATKKAFEQK
                     LKEAMNQYHNHNLSVLEILEELIALAKEFEARQKRGEALGLSPAEMAFYDALARNESA
                     VREMGDEVLMKLAKDITDKLRKSVTVDWQYKDSVRAKMRTLIRIALRTYKYPPDLQAE
                     AIEFVLQQAEEIAGEFAITETA"
     gene            complement(3302..6193)
                     /gene="glnE"
                     /locus_tag="PXB27_001307"
     CDS             complement(3302..6193)
                     /gene="glnE"
                     /locus_tag="PXB27_001307"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HDL5346095.1"
                     /translation="MLTTLIPLSQLNLPPNLQNSEQIQPLVTAISLSNFVHQTLQKQP
                     DLLAEWLAKFPTPDDCCNYTERLAKILARVENEEMLGRVLRQFRHRELARLSFIQSNK
                     LATVELVFQHLSDLAESLILAARDWLFQRCCAEYGTPKNTLGETQELLILGMGKLGGR
                     ELNFSSDIDLIFTYPDIGETEGGRKSIENSKFFTRMGQRLIKVLDEITADGFVYRTDM
                     RLRPFGDSGALVLSFTAMEDYYQEQGRDWERYAMIKAKILGEDSQNLNHRYLKQMLRP
                     FVYRRYLDFSAIQSLREMKEKISREVVRRNLTDNIKFGAGGIREVEFIAQTFQMIRGG
                     RDKILQERSLLKVLPRLAELNLLTQTQVETLHNAYIFYRQIENVLQAIDDKQTQTLPT
                     DEASQARLVFACQSYYQQDLYSEKTHWVEHSFNDWQQFMAVLSQYQQAVRQIFNEIIG
                     EEETQANCNPVNEKLAEWKDILHYNIRLEDLSAVLKGYPKVAENDYTEIFRHFSTTFQ
                     DWVKRPIGVRGREVLRNLMPRIADSIFKGEDHLLLLPRVLNIVDKITTRTTYLELMLE
                     KEQILPQLLALCSKSVMIAEQIARYPMLLDELMSHRGLTDVQAFEKYQSALQDYLIRI
                     PEEDEEALIDGLRQFKQTQILRIAAADILGVLLVMKISDHLTYLAEAIINAVVNMAWK
                     QVSQRFGVPEHLEADEKGFAVIGYGKLGGIELGYNSDLDLVFLHNAPEDSQTVGGKKE
                     ISSHQFYLKLAQKINSIFNLNTSAGVLYEVDMRLRPSGEAGLLVSTFNAYEHYQKNEA
                     WTWESQALVRTRCVFGAENLKQAFEKIRQSTLAQPRASGQLRQEICEMRQKMYQHLSS
                     HSAEQFHIKQDQGGITDIEFIAQYLVLAHSHQHPKMAVWSDNVRIFDSAVECGILSSE
                     QSEQLQHCYTALRNKIHHLNLLRKDSVVDASEFTTERAFVREMWQRLLA"
     gene            complement(6365..6646)
                     /locus_tag="PXB27_001308"
     CDS             complement(6365..6646)
                     /locus_tag="PXB27_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HDL5346096.1"
                     /translation="MYYVIFAQDNPNMLEKRLEVRPAHLARLEKLQAEGRLLIAGPNP
                     TEDGSSVTGSTVIAEFNSLEEAQQWASQDPYVEAGVYSDVIIKPFKRVF"
     gene            complement(6648..7112)
                     /gene="yciA"
                     /locus_tag="PXB27_001309"
     CDS             complement(6648..7112)
                     /gene="yciA"
                     /locus_tag="PXB27_001309"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HDL5346097.1"
                     /translation="MKDKPYGKAPEGKLILRTLAMPSDTNANGDIFGGWIMSQMDLGG
                     AILAKELAKGRVVTVTVDKMIFHRPISVGDVVCCYGKLMHIGRSSMQVKVEVFIKQVY
                     EGNRERYLVTEALFTYVAIDKEGKPRAVPRENNPELDEALSLIDLTPEKQGE"
     gene            complement(7102..7659)
                     /locus_tag="PXB27_001310"
     CDS             complement(7102..7659)
                     /locus_tag="PXB27_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HDL5346098.1"
                     /translation="MKQLLEFIPLILFFVVYKMSGIQMASIALVIATIVQMIALKMLY
                     GKIEKQQIIMGGSVVFFGLLSAYFNEVKYLQWKVTIVYALFALVLLVSQFVFKTPLIK
                     KLLGKEIQLPEAVWNKINFGWAVFFLICMLLNIYISQYLSEEIWVDFKSFGIIGLTLV
                     ATVITGFVLYPYLKDAEKQEQKNER"
     gene            complement(7668..8477)
                     /locus_tag="PXB27_001311"
     CDS             complement(7668..8477)
                     /locus_tag="PXB27_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5346099.1"
                     /translation="MAQALLYFTTKYLQSIEGYFMSIKFVDLLQQSWNFMRNQQAFSL
                     FAIVTIVVVQLVFILVSPNSPELMAESQPQQLQIDASKMVSILLPTILLGVVNLLINV
                     LMIFNIQSINDGNYRQFFQNAGNALKHFLPVLLLQFVMVLPLSLGASFAMASPETVII
                     ALPVLVVGFYFFIKLSLVIYAYLLEKPQKSLSESIKFTLQLSRGKMLPLILFCVISYL
                     LPGMLSRLFTVFGNDFIGIFITIVLSAVINVFMAIFSFRFYQVYRQMPAVR"
     gene            8548..8985
                     /locus_tag="PXB27_001312"
     CDS             8548..8985
                     /locus_tag="PXB27_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HDL5346100.1"
                     /translation="MLSLFDAGQRYLNTLPNQKKLANFMPDYHIIRLVKFSSKVMPAF
                     ACFALVWQYCFTDPTQSILANSVLTAVFAISIPFQGLYWLGKRARSPLSLSLLEWYES
                     LRQKLISAQVKLEETQAVPSFQDFANLLKLAEETWGDEYFDEL"
     gene            complement(9105..9180)
                     /locus_tag="PXB27_001313"
     tRNA            complement(9105..9180)
                     /locus_tag="PXB27_001313"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9145..9147),aa:Asn,seq:gtt)
     gene            complement(9296..10045)
                     /gene="moeB"
                     /locus_tag="PXB27_001314"
     CDS             complement(9296..10045)
                     /gene="moeB"
                     /locus_tag="PXB27_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HDL5346101.1"
                     /translation="MELTDHEMLRYNRQIILKSVDFDGQEKLKASRVLIVGLGGLGCA
                     ASQYLASAGVGHLTLVDFDLVSLSNLQRQILHTDATIGQPKVESAKARLAAINPNIQI
                     ETINQRCSDEQLAKLIANVDLVLDCTDNVTVRNQLNLQCFKQKRPLVSGSAIRFEGQV
                     SVFCYQEQEPCYECLSRLFGDATLSCVEAGVIAPIVGIIGSIQALEAIKVLLNIGRTL
                     SGKLLMIDGLHFSVREMALPKQNNCPVCTSY"
     gene            complement(10144..11373)
                     /gene="moeA"
                     /locus_tag="PXB27_001315"
     CDS             complement(10144..11373)
                     /gene="moeA"
                     /locus_tag="PXB27_001315"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HDL5346102.1"
                     /translation="MALLALSDALDNMLARLSTPKLVEQLPLNECANRVLAEDIFSPI
                     NVPNFDNSAMDGYAVRISDLAQNLTLNVAGKAFAGNPFNGEIKAGECVRIMTGALVPK
                     GVDAVVMQEETTLNSDGSVTFNHIPKLGSNIRRIGEDVKQGALVLKKGSLLNVASLPL
                     LASLGIASVPVFVKLKVAILSTGDELTSVGEPLSEGKIYDTNRFAVRLMLEKLNCEIL
                     DFGILPDDPILFEQTFRQAQQQADVLITSGGVSVGEADFTKDVLEKLGEIGFWKIAMK
                     PGKPFAFGKLEQAWFFGLPGNPVSALVTFYQLAQPALAKLSGLSTDKIAKLTQNLTAC
                     SAGKLKKAVGRQDFQRGYYYANEKGELEVRAVGTQGSHIFSAFNESNCFIVLEAERGN
                     VDAGEKVTIQPFNSLMY"
     gene            complement(11395..12012)
                     /locus_tag="PXB27_001316"
     CDS             complement(11395..12012)
                     /locus_tag="PXB27_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006719828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HDL5346103.1"
                     /translation="MFDSLAVQFVVLWAVIDPIGSVPVYLAKTIGLPIEARRKIALNA
                     VLIAAGILIFFLIGGQVLLESMQIPLPAFQAAGGLVLLLFALTMIFGESKPEQEMRIS
                     SNLNELAVYPLAVPSIASPGAMMAIVLLTDNHRFHIGDQIITAGIMLLVLFITYLLLL
                     AANRIQHLIGNAGAAIISRVMGLILAAIAVNNLLIGVRDFFVQIS"
     gene            complement(12080..13291)
                     /locus_tag="PXB27_001317"
     CDS             complement(12080..13291)
                     /locus_tag="PXB27_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL5346104.1"
                     /translation="MISRQMQSLLKRLVAQFPAVLVTGARQVGKSTLLQHIGEGYQYI
                     TFDDPMLLELAKQESRLFFLNHQGKLILDEVQYVPEIFALLKLEIDKKQQNGLFLLSG
                     SQAFELMQNVSETLAGRIAILKLQGLSLREILGVKFDQPFLPTTDYLLAREQELKSPE
                     NIWQLIHKGDMPRLYAQEVDWEIYYRSYVATYIERDVRQLTNIASTTDFTRFMVSIAS
                     RSGELLNYSNIAQEVGVSNETIKRWVAILQTSGIIYLLQPYHNNHLKRAIKTPKVYFL
                     DTGLMAYLTKWLTPETIAQGAKSWQFFETFVVSQIIKSFHNQGIEPPIYFYRDTNQKE
                     IDLIIEYDRTLYPIEIKVTASPTKKMAKSFDLLNSVSSEWKLGQGAIINQYPTKLYLS
                     ENLVALPVSYL"
BASE COUNT         3996 a         2924 c         2698 g         3699 t
ORIGIN      
        1 ttgaaggtat ttttaataag ataaaagaaa atattattca aaataataac ttatctaaaa
       61 ttagagataa gttattacct aaattattaa gtggggagat ggagttgtga tgattaccat
      121 caacgaaaac accattgaac aatccgccat tgcaactttg caaagcttgg gctgggacta
      181 cacctacggc aaaaaaattt tggcaggttt agaacacgaa tggcgggagc gaaccgctga
      241 ggtgatttta aagccgcttt tggcgcaagc gattgcaaaa ttcaacccga atttgcccgc
      301 ttgtgaggtg gaaaatgtgg tggcacaggt gtgccgtgcc gacagtggcg atttggcgga
      361 gcgtaatcgt caggcttatg attggctaag aaatggggtc aaaatcactt atcagttgta
      421 cggtgagcag gtgtctgatg tggtgcagct gattgatttt cagcgtccag aaaataacga
      481 ttttcgcatt gtcaatcagc tggatattag cggcaaaaaa ggcaaacgca ttccggattt
      541 gattggcttt gttaatggct tgccgctggt ggtgtttgag ctgaaaaatc cgctcaaaga
      601 aaatgccgac attggcaagg cgtttgccca actgcaaacc tataaagatg aaatttctga
      661 tttgtttgtg tttaatcagg cactcgtgat ttcagacggc attgtcgccc gcatcggttc
      721 gctgaccgct gatttcgacc gttttacccc ttggcgtgtg gttgatgaaa aaaatcagag
      781 caaacgcatt gtgtttgaag atgaactcac cgccctgctg caaggcgtga tgacacccaa
      841 aaatctcttg gattatgtgc agaattttgt ggtgtttgaa cgggacggca aaaaccgctt
      901 aattaagaaa atcggagcat atcatcagtt ttatggcgtg aatgaagcgg tggattgcac
      961 cttgcttgcc gccacaggca accgcaaaat cggcgtgttt tggcatacgc aaggttcggg
     1021 caaatcgctt tcgatgctgt tttatgcagg taaagtatta agccaaagca gcctgaaaaa
     1081 tccaactttg gtgttggtaa ccgaccgcaa cgatttggac ggtcagcttt acgccacttt
     1141 ttgcggtggc gaggcactgc tcaaacaaac gccaatccaa gccgatgggc gagacgaact
     1201 ccgctctgcc cttgccagtc gttcggcagg cggtgtgatt tttaccacca ttcaaaaatt
     1261 cggcttaatg gagggtgagc ttgcccaccc tgtattaaac gagcgggaaa acatcattgt
     1321 gattaccgat gaagcacacc gctcacaata tggttttagc caaaaaatca accacaaagg
     1381 cgagtatcgt gagggttatg ccaagcattt acgcagtgca ttgccgaatg cctcttttat
     1441 tggctttact ggcacaccga ttgaacttga tgacaaagac acccaagaag tcttcggtaa
     1501 atatgtctcg atttatgatt ttgaagatgc ggtggaagat ggggcaaccg tgccgattat
     1561 ttatgagcca cgccaaatca gcttaggcga aagtggcgag ttttccaaag tgatggaaga
     1621 ggcacaacaa ctgattgatg acgatgaaaa cagctataac ttccgcctgc gtgaaaaact
     1681 gcacagcgtg gatagccgtt tgcaaaaaat ggcggaagat attattgccc attacgatga
     1741 gcgtaccaaa cagcaagacg gcaaggcgat ggttgtggtg atgagccgtg ccatttgcgt
     1801 gaaattgtac gaaaaaatca ccgcacttcg tccagaatgg cactcaaacg atgtgcttca
     1861 aggcagcatt aaaattgtga tgacaagtaa tgccagcgac cctgccgagt ggcaaaaaca
     1921 caatcaggat aagaaaacct tagaaaaacg ctttaaagac ccagacgatc cgctcaacat
     1981 cgtgattgtg cgggatatgt ggctgacagg ctttgatgcg ccctgctgta acacaatgta
     2041 cattgataaa ccaatgagcg gacacaacct gatgcaggca atcgctcggg taaatcgggt
     2101 atttcgcaac aaaagccgtg aaaatggcgg cttgattgtg gattatgtgg gcttgaccga
     2161 tgagctggaa aaagcgatga agcagtacac caacgcaggc ggtaaagaaa agccggtgcg
     2221 ggacatttca gcggtgctgg aaaaaatggt ggaacatatc acggtcattc gtgggcaatt
     2281 tgccacaccg attgacggac aagcggttga tattgccaaa atgttgcaaa tcagcgaacc
     2341 gcctaaactg ctcaatgcga ttttgacatc agccaaccat attcttgccc tagaccgcat
     2401 tcagccacct gataacactg ccaaagacaa aaccccacgc aaaaacgcct ttttgcaatc
     2461 ggtacgcttg gcgaaaaaag gttatgcttt gtgtggggca ttaaaagcag ttgaacccta
     2521 taaacaggaa ctggcgtttt atgatgcagt gcgtgccacc attatcaaaa acagcaccgc
     2581 tcctcgcaat tcttcgagcg aaaatgaccg cttgttgcag cttaccgcct tgatgaatcg
     2641 tgcggtacag tcggatggtg tggtggattt atttgatttg ctgaaaaaag accgcccaaa
     2701 catcaacctg ctttctgatg agtttttgga aacagtaaaa aacagcccaa ccaaagattt
     2761 gtggctatca gcaatggagc gttatcttgc ctcacaactg cgagagcaaa gcggtgccaa
     2821 tcttgccaca aaaaaagcat ttgaacagaa actcaaagag gcgatgaacc aataccacaa
     2881 ccacaattta agcgtattgg aaattttaga agagctgatc gccctcgcca aagagtttga
     2941 agcccgccaa aaacgagggg aagcgttggg actaagccct gcggaaatgg cgttttatga
     3001 tgctttggca cgtaatgaaa gtgcggtgcg ggaaatgggc gatgaggtat tgatgaaact
     3061 tgccaaagac attaccgata aattacgcaa atcggtcacc gtagattggc aatataaaga
     3121 ctcggttcgt gccaaaatgc gaaccttaat ccgcattgct ctgcgtacct ataaataccc
     3181 gcccgattta caggcggaag cgattgagtt tgtgttgcaa caagcggaag agatcgccgg
     3241 agagttcgct atcaccgaaa cggcgtaatc tatattaggg cgtgcaatgt acgccctaaa
     3301 tttatgctaa taacctctgc cacatttccc gcacaaatgc tcgctcggtg gtaaattcac
     3361 ttgcgtccac cacgctgtct ttgcgtaata agtttaggtg gtggattttg ttacgcagtg
     3421 cggtgtagca atgttgtagc tgttcgcttt gttcggaact taaaataccg cattctactg
     3481 cgctgtcgaa aattcggaca ttatccgacc atactgccat tttcgggtgt tggtggctgt
     3541 gggctaatac caaatattgg gcgataaatt caatatcggt aatacctcct tgatcttgtt
     3601 taatatgaaa ttgctcggct gaatgcgagc ttaaatgttg gtacattttc tgtcgcattt
     3661 cgcaaatttc ttggcgtaat tgaccgcttg cacgaggctg agcaagcgtg ctttggcgga
     3721 ttttttcaaa ggcttgtttg aggttttccg ccccaaatac acatcttgtc cgcacgaggg
     3781 cttggctttc ccacgtccac gcttcatttt tttgataatg ctcgtaggca ttgaaagtgc
     3841 tgactaaaag cccagcctcg cctgatgggc gaagtcgcat atccacttca tatagtacgc
     3901 cggcactggt attaaggtta aaaatcgagt taattttttg agccaatttt aagtaaaatt
     3961 gatggcttga aatctctttt ttaccgccaa cagtttggct atcctctggg gcattatgca
     4021 agaaaaccaa gtcgagatct gaattatagc ccagttcaat cccgcctagc ttgccgtagc
     4081 caataactgc aaaccctttt tcatcagcct ctaaatgttc cggcacacca aagcgttggc
     4141 tgacttgctt ccacgccata tttaccaccg cattaataat cgcttctgct aagtaagtaa
     4201 gatgatcgct gatcttcatt accagcagca cgcctaaaat atcagcagcg gcgatgcgta
     4261 aaatttgggt ttgtttgaac tgacgtaagc catcaatcag tgcttcttca tcttcttcag
     4321 gaatgcggat taaataatcc tgcaaggcag attgatattt ttcaaaagcc tgcacgtcgg
     4381 ttaagcctct atggctcatc agctcatcaa gtagcattgg atatcgagct atctgttccg
     4441 caatcataac cgacttacta cacaatgcta aaagttgagg cagaatctgc tctttttcga
     4501 gcattaattc caaataggta gttcgggtgg tgattttatc gacaatattc aacacgcgag
     4561 gcaataacaa taaatgatct tcacctttaa aaatgctgtc ggcaattcta ggcattaaat
     4621 tgcgtagcac ctctcgccct cttaccccaa tcgggcgttt tacccaatct tgaaaggtag
     4681 tagaaaagtg acggaaaatc tccgtataat cattttctgc aacctttgga taacctttta
     4741 gcactgcgga taaatcttca aggcgaatgt tatagtgcaa aatgtctttc cattcggcta
     4801 atttttcatt aaccggattg cagttggctt gtgtttcctc ttcaccgata atttcattaa
     4861 aaatctggcg tactgcttgt tgatattggc tcaatactgc cataaattgt tgccaatcgt
     4921 taaaggaatg ctccacccaa tgcgtttttt cgctatatag atcttgctga taatagcttt
     4981 ggcaagcaaa tacaaggcgg gcttgcgagg cttcatcagt gggtagggtt tgagtttgtt
     5041 tatcgtcaat cgcttgtaat acattttcaa tttgacggta aaaaatatag gcattgtgga
     5101 gcgtttccac ctgcgtttgg gttaataaat ttaattctgc caagcgtggt agcactttta
     5161 acaggctacg ttcttgtaaa attttatctc gcccgccacg gatcatttgg aaagtttggg
     5221 cgataaactc cacttcccga atcccaccgg caccaaattt aatattatcg gttaaattac
     5281 ggcgaaccac ttcacggctg attttttctt tcatttcacg caacgattgg attgcactga
     5341 aatccagata acggcggtaa acaaaagggc ggagcatttg ctttaaatag cgatgattta
     5401 aattttggct atcctcgcct aaaatttttg ctttaatcat cgcataacgt tcccaatcac
     5461 gcccttgctc ttggtaataa tcttccattg cagtaaaact cagcaccaag gctccgctat
     5521 caccaaaggg gcgaagtcgc atatcggtac gatacacgaa accatcagcg gtgatctcat
     5581 ctaatacttt aattaaccgc tgccccattc gggtaaaaaa tttgctgttt tcaatcgatt
     5641 tgcgtccacc ttcggtttca ccaatatcgg ggtaggtaaa aatcaggtca atatcggaag
     5701 aaaaattaag ctctcgtccg cccaatttgc ccatacctaa aatcagcagt tcttgtgttt
     5761 cgccaagagt gttctttggc gtgccatatt cggcacagca acgctgaaac aaccaatctc
     5821 gagcggctag aatcaggctc tcggctaaat ccgacaaatg ctgaaatacc aattctaccg
     5881 tcgctaattt atttgactga ataaagctca gacgagccaa ttcacggtgg cgaaattgcc
     5941 gcaaaaccct ccccaacatt tcttcatttt ccactcttgc taatattttt gctaaacgct
     6001 cagtataatt gcagcaatca tcaggcgtag gaaacttggc aagccattct gccagtaaat
     6061 ccggctgttt ttgcaaggtt tgatgcacga aattggaaag cgaaatcgcc gtcacaagcg
     6121 gttgaatttg ctcagaattt tgcaaattgg gcggtaaatt gagttgggat aagggaatta
     6181 gggtggtaag cataaattta ggctcaaaat tgaaaataga tagaatttta cgctcgatca
     6241 attcccctct ttagcaaaga gggcttaggg gagatttggc agaggtaatt ttactctcat
     6301 acgaaacatt aatatcgtaa tcaaatctcc cccagcccct cttttctaaa gaggggagaa
     6361 aaaattaaaa cactctttta aacggtttaa taatcacatc gctatacaca ccggcttcca
     6421 cataaggatc ttggcttgcc cattgttgag cttcttcaag tgagttgaac tctgcaatta
     6481 cagttgaacc ggttacgctt gagccgtctt cagtcgggtt aggccctgcg attaataatc
     6541 gaccttctgc ctgtaatttt tccaaacgag ccaaatgagc cgggcgaact tctaaacgtt
     6601 tttccaacat attgggatta tcttgggcaa agataacata atacatacta ttctccttgt
     6661 ttttcagggg ttaaatcaat tagcgataat gcttcgtcta attcggggtt attttctctc
     6721 ggcacagcac gcggtttgcc ttctttatca atcgctacat aggtaaataa ggcttcggtt
     6781 acgagataac gttcgcgatt gccctcataa acttgtttga taaagacctc gactttcact
     6841 tgcatcgaag aacgccctat gtgcattaac ttgccgtagc agcagaccac atcaccgact
     6901 gaaatcgggc ggtggaaaat cattttatct accgttaccg taacgactct accttttgcc
     6961 aactctttgg ctaaaattgc accgcctaaa tccatctgcg acataatcca accgccgaaa
     7021 atatcgccat tggcgttagt atctgacggc attgccaaag ttcttaaaat cagtttccct
     7081 tccggggctt ttccataggg tttatctttc atttttttgt tcctgctttt cagcatcttt
     7141 taagtaaggg taaaggacga aaccggtaat aaccgttgcg actaaggtta agccaataat
     7201 gccaaaggat ttgaaatcaa cccaaatctc ttccgataaa tattgactga tataaatatt
     7261 cagcagcata caaatcagaa agaatactgc ccaaccgaag tttattttat tccacactgc
     7321 ttcaggcaac tgaatttctt tgcctaataa ttttttgatt aatggtgttt tgaacacaaa
     7381 ttggctcact aatagcacca atgcaaacag agcgtaaaca atggttactt tccattgtaa
     7441 atatttaact tcattaaaat aggcactcag taagccgaaa aagaccaccg agccacccat
     7501 aataatttgc tgtttttcaa ttttgccgta gagcattttt aaggcgatca tttgcacgat
     7561 tgtagcgata accaatgcga tagacgccat ctgaataccg ctcattttat acacgacaaa
     7621 aaacagaatc agcggaataa attcaagtaa ctgtttcatt gatactccta acgcactgcc
     7681 ggcatttggc ggtaaacttg gtaaaagcgg aagctgaaaa tcgccataaa gacgttaatt
     7741 accgcactta aaacaatggt aatgaatatg ccaataaagt cattaccaaa cactgtgaac
     7801 aaacgagata acatccccgg taataaataa gaaatcacac aaaataaaat cagcggtagc
     7861 atttttccac gtgataattg aagcgtaaat ttaatacttt ccgatagact tttctgcggt
     7921 ttctccaata aataagcata aatcactaac gataatttga taaaaaaata aaaaccgact
     7981 accaacaccg gcaatgcaat aatcactgtt tcaggcgatg ccattgcaaa agatgcaccg
     8041 agagaaagcg gtaacaccat cacaaattgt aacaacagca caggtaggaa gtgctttaat
     8101 gcgtttcctg cattttggaa aaattgacga taattgccat cgttaatcga ttggatattg
     8161 aaaatcatta agacattgat caaaagattg acaacaccta ataaaatagt tggcaatagt
     8221 atagacacca ttttagaggc atcaatttga agttgttgcg gttgcgactc cgccattagc
     8281 tcaggcgaat ttggcgacac taaaataaaa accaactgca caaccacaat ggtgacaatg
     8341 gcaaacagtg aaaatgcttg ttgattacgc ataaagttcc aactttgctg caataaatcg
     8401 acaaatttaa ttgacataaa atacccttca atagattgta aatattttgt tgtgaagtat
     8461 agcaaggctt gagccaaact gcattaacct attgtagatt ttaatcagtt agctaaaata
     8521 aaagaaaaat ttgaaagaga taatcctatg ttaagcttat ttgatgcagg acagcgttac
     8581 ctcaacactc tgccgaatca gaaaaaatta gccaatttta tgccagatta ccacattatt
     8641 cgcttggtaa aattttcctc taaagtaatg cctgcttttg cttgctttgc attagtttgg
     8701 caatattgct ttaccgatcc gacccaatct attttagcca attcggtact tactgctgtt
     8761 ttcgccattt ctattccgtt tcaagggtta tattggcttg gaaaacgggc aagatcaccg
     8821 ttatctttat cattattgga atggtatgag tcgcttagac aaaaattaat ttcggcacaa
     8881 gtcaaattag aagaaacaca agcggtacct tctttccaag attttgcaaa tttactcaag
     8941 ctagcagaag agacttgggg agatgaatat tttgatgagt tataaaataa aaaaagcccc
     9001 acttaaaata agtggggtaa aacttttgtt ctaattctgg aggtttgaat tactgtttta
     9061 taaaaggaga caaataaaac aagaacaaaa gaaaaataaa atcttggctc ctcctgcggg
     9121 actcgaacct gcgacatatg gattaacagt ccaccgttct accgactgaa ctaaggagga
     9181 attaacggtg taaataatag gctaaaacgt tttagccgtc aaataaaaaa aataaactgt
     9241 cgttcatttt ttacatttct tgacattttt tcgccaataa gtatgagaaa aatacttaat
     9301 aagaggtaca aaccggacag ttattttgct taggtaatgc catttctctg acagaaaaat
     9361 gcaagccgtc aatcattaac aattttccgg aaagagttct gccgatatta agtagcactt
     9421 taattgcttc caacgcttga atactgccta tgatgccaac aatcggagca atgacccccg
     9481 cttcaacaca acttaaagtc gcatcaccaa acaacctgct taaacattca tagcacggtt
     9541 cttgttcttg ataacaaaat acactcacct gcccttcaaa tcgaattgcc gaaccggaaa
     9601 caagcggtcg tttttgctta aaacattgca aatttaactg attacgcaca gtaacgttat
     9661 cggtacaatc cagcactaaa tctacatttg caatcagctt ggctaactgc tcatcggaac
     9721 atcgctgatt gatagtttca atctgaatgt tcggattaat cgccgctaat cgagctttgg
     9781 cagattccac ctttggctgc ccgatagtcg catcggtatg taaaatttgg cgttgcagat
     9841 tagaaagtga gaccagatca aaatccacca aagtcaaatg cccaacgccg gcagaagcta
     9901 agtattgcga tgccgcacag cccaaaccac ctaaaccgac aatcaacact cggctggctt
     9961 ttaacttttc ctgcccgtca aaatccacgc tttttaggat aatctggcga ttataacgta
    10021 gcatttcgtg atcggtaagt tccattttta cacctaaaga gtaaacgatg gcgcaagcgt
    10081 ccacgcttgt gcctatattt ttattgctag cacaagcgtg gacgcttgcg ctatcttaat
    10141 tcattaatac atcagcgaat taaacggctg aatagtgact ttttccccag catcaacatt
    10201 gcctcgttct gcttctagca caataaaaca gttactttca ttaaaagcgc tgaaaatatg
    10261 tgagccttgc gtgccaactg cacgaacttc taactcacct ttttcattgg catagtaata
    10321 gccccgttgg aaatcttggc gaccgaccgc ttttttcaat tttccggcac tgcaagcggt
    10381 tagattttgg gttaattttg caattttatc tgtcgataaa cccgaaagtt tggctaacgc
    10441 cggttgcgct aattggtaga aagtcactaa tgcagagaca gggttgcccg gcagcccaaa
    10501 aaaccaagcc tgttctaatt taccgaatgc aaaaggcttg cccggcttca tcgcaatttt
    10561 ccagaaaccg atttcgccta atttttccag tacatctttg gtaaaatctg cctctcccac
    10621 agatacgccg ccactggtaa tcaacacatc ggcttgctgt tgggcttgcc tgaaagtttg
    10681 ctcaaacaga attgggtcat caggcaaaat cccaaaatct aaaatctcgc agttgagttt
    10741 ttccagcatt aaacgcaccg caaaacggtt ggtgtcgtaa atttttccct cactcaaagg
    10801 ctcgccaact gaggttaatt catcgccggt tgaaagaatt gccactttta attttacaaa
    10861 aacaggcaca gaagcaatac cgagtgaagc tagcaacggt aaactggcta cattcaataa
    10921 tgagcctttt ttcaagacca atgccccctg tttaacatct tccccaatgc ggcgaatgtt
    10981 actgcctaat tttggaatat ggttaaaggt tacagagcca tcggaattta acgtagtttc
    11041 ctcttgcatt acaacggcat caacgccctt tggcactaat gctcccgtca taatccgcac
    11101 gcattcgcct gcctttattt cgccgttaaa cgggttaccg gcaaaggctt taccagccac
    11161 attcaaggtt aaattttgtg ctaaatctga aatacgaacc gcgtagccgt ccatcgccga
    11221 attatcaaaa ttcggtacat taatcgggga aaaaatatcc tctgccaaca ctcggttagc
    11281 acactcattt aacggaagtt gttccaccaa tttaggtgtc gataaacgag caagcatatt
    11341 atctaacgca tcactcaaag ccaaaagtgc cataatttcc tacctattca acgtttagct
    11401 aatttgtaca aagaaatccc gtacgccaat caacagatta ttcaccgcaa tcgcagccag
    11461 aattaacccc atcactcggc tgataatcgc agcccctgca ttaccaataa ggtgctgaat
    11521 acggtttgcc gctaatagca acaaataggt aataaagagt accaaaagca taatgccggc
    11581 ggtgataatt tgatcgccga tatgaaaacg gtgattgtcg gttaataata cgattgccat
    11641 catcgccccg ggagaagcaa tcgacggtac agctagcgga taaaccgcca attcattcag
    11701 attactgctg atccgcattt cttgctcggg cttgctttcg ccaaaaatca tcgttaaagc
    11761 aaataacaac agcactaagc cgcctgccgc ttggaatgca ggtaacggaa tctgcattga
    11821 ttctaacagc acttgcccac cgataaggaa gaaaatcaaa ataccggcgg caatcaagac
    11881 cgcatttagt gcaattttac gccgtgcttc aatcggcaaa ccaatggttt ttgccaaata
    11941 aacaggaaca gaaccaatcg ggtcgatcac cgcccataaa accacaaatt gtaccgctaa
    12001 tgaatcaaac atattctctc ctgttatctt gataatttgc aaaattttag cataaaacaa
    12061 ccgcttgcac ggctttttct tacagatagc tcaccggtaa cgctaccaaa ttttcactta
    12121 aataaagttt ggtcggatat tggttaataa tcgctccctg ccctaatttc cattcactag
    12181 aaactgagtt tagcaagtca aaactttttg ccattttttt ggttgggctt gccgtgacct
    12241 taatttctat cggataaagc gtgcgatcat attcaataat taagtcgatc tctttctggt
    12301 tagtatcacg gtaaaaataa atcggcggct caattccttg attatgaaag gattttataa
    12361 tttggcttac cacaaaggtt tcaaagaact gccagctttt tgccccttga gcaatagttt
    12421 cgggggtaag ccatttggtt aaatacgcca ttaacccggt gtctagaaaa tacacttttg
    12481 gggtcttaat tgcccgtttc aaatgattgt tgtggtatgg ctggagcaaa tagataatgc
    12541 cggaagtctg caaaatggcg acccaacgtt taatcgtttc attcgatact ccaacttctt
    12601 gagcaatatt actgtaattg agtagctcac cactacgaga ggcaatagag accataaaac
    12661 gggtaaaatc cgttgtgctg gcaatattgg ttaattggcg aacatctcgc tcaatgtagg
    12721 tcgctacata agagcgataa taaatctccc aatccacctc ttgtgcatat aaacgaggca
    12781 tatcgccttt atggataagc tgccagatgt tctccggtga ttttaattct tgctcccgag
    12841 ctaacaaata gtcggttgtc ggtaaaaaag gttgatcgaa cttcacccct aaaatttcac
    12901 gcaacgaaag cccttgtaat tttaaaatag caatacgacc ggcaagtgtt tcactcacat
    12961 tctgcattaa ttcaaaggct tgcgaccccg aaagtaaaaa caaaccgttt tgctgttttt
    13021 tatcaatttc taactttaat aacgcaaaaa tttcaggcac atactgtact tcgtctaaaa
    13081 taagcttgcc ttgatggttt aaaaaaaaca ggcgagattc ttgtttagcc agctccagca
    13141 acattgggtc atcaaaagta atatattgat agccctcacc aatatgttgt aacaatgtcg
    13201 atttaccaac ctgtcttgcc cctgtcacta aaacggcagg aaattgcgcc acgagtcgtt
    13261 ttaacagcga ttgcatttgc cgagaaatca tttttcctcc aaaatagcct aaatcaa
//
LOCUS       DANLUQ010000059        13206 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.059, whole genome shotgun sequence.
ACCESSION   DANLUQ010000059 DANLUQ010000000
VERSION     DANLUQ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 13206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13206
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.059"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            116..1021
                     /locus_tag="PXB27_001318"
     CDS             116..1021
                     /locus_tag="PXB27_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDL5346105.1"
                     /translation="MSQKYSVAVIGLGAMGMGAAKSCIAAGLDTYGVDLNPNYLETLK
                     AAGAKKVGTSAVDFAAELDAVLLLVVNANQVNAVLFGENGLAQHLKQGTVVMVSSTIS
                     AQDAKNISQKLTDLGLVMLDAPVSGGAAKAAQGEMTVMASGSSEAFEKLKPVLDAVAG
                     KVYNIGEEVGLGATVKIIHQLLAGVHIAAGAEAMALAARAGIPLDLMYDVVTNAAGNS
                     WMFENRMKHVVEGDYTPLSMVDIFVKDLGLVNDTAKSLRFPLHLASTAYSMFTEASNA
                     GYGKEDDSAVIKIFSGIELPKKEGK"
     gene            1023..2264
                     /locus_tag="PXB27_001319"
     CDS             1023..2264
                     /locus_tag="PXB27_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005754893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HDL5346106.1"
                     /translation="MLGIIADDFTGASDIASFLTENGLKTVQMNGVPKTTLNSQVDAI
                     VISLKSRSNPVEEAIQQSLQALDWLKQNGCSQFYFKYCSTFDSTEKGNIGPVTDALMA
                     ALDTDFTVVTPALPVNGRTIFNGYLFVGDVLLNESGMRNHPITPMKDANLVRLMDAQS
                     QGKTGLVAYADVIQGAERVKARFAELKQQGYKYAVVDAADNSQLAVLAEAVADFQLVT
                     GGSGLGAYMAAYRSGGKKGTNAFVPSKGKTVVLSGSCSVMTNTQVAAYQQKAPSYFLE
                     AEKAIQDPNYVETLYQWVIANLDGDFAPMVYATVPPEKLKEIQTQFGGDKASHAIENT
                     FAKLAEKLQAAGVTNFITAGGETSSIVVQQLGFSGFHIGKQIAPGVPWLQAVEKPIFL
                     ALKSGNFGKEDFFSFAQEMFV"
     gene            2261..2893
                     /locus_tag="PXB27_001320"
     CDS             2261..2893
                     /locus_tag="PXB27_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014668373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDL5346107.1"
                     /translation="MTEQEQKIEMVNLARSLFERGYTVGGAGNLSVRLDENRILVTPT
                     GSSLGRLEADRLSVLDMQGNVLSGDKPSKESVFHLAMYQKNPACQAIVHLHSTYLTAL
                     SCLENLDTDNAMRAFTPYYVMRVGKLPVIPYYRPGDINIARELGERALDGKAFLLANH
                     GVVVTGSDLRDAVDNTEELEETSKLYFILQGQKVRYLTDDEVNDLKNRGK"
     gene            2895..3671
                     /locus_tag="PXB27_001321"
     CDS             2895..3671
                     /locus_tag="PXB27_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005547735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase family protein"
                     /protein_id="HDL5346108.1"
                     /translation="MPKFAANLTMMFNEVPFLDRFEAAANAGFKYVEYLWPYDYPAAE
                     LKAKLEQYGLKQVLFNTAAGDVAQGEWGVSAIPGREADSHRDIDLALEYALALGCENV
                     HVMAAVVPEGEDREAYKQTFIKNVQYAADKFKPHGINVLLEALSPEVKPNYLLKSQFD
                     TLEIVEAVNRDNVFVQLDYFHAQNVDGNLSRLTDKLKGKFAHIQIASVPDRHEPDEGE
                     INYQYIFNKLDEIGYEGYVGCEYKPRAETTSGLGWFEQYK"
     gene            3724..5082
                     /locus_tag="PXB27_001322"
     CDS             3724..5082
                     /locus_tag="PXB27_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntT protein"
                     /protein_id="HDL5346109.1"
                     /translation="MSNEMLILIGLASVVALLVIMIKGKVHPFLALGLVSIAVALTAG
                     IPMSKVIPTLISGMGSTLGSVALIVGLGAMLGKIIEKSHGADVLANWLLQRFGEKNAP
                     FALGMTGFIFGIPVFVDVGFIVLLPIIFSVARRLGGNILVYSLPIGLAMLLVHVLVPP
                     HPGIVAGAQNLNADIGFVLGIGLVAALPAYLIGQKFIPFFTKNNFVKVVTSTDLLQHQ
                     KDLIKEKGIELPSFGKVLAMISFPLILIMLGTVTATTLPKEHIVREVFSMVGSSPFAL
                     FVAVCLSAYVLGFRYGWTKHKLEEVLESALAPIAGIILITGAGGMFGKVLDASGVGKA
                     LADVLANTGLPLLVLSFILSALLRAAQGSATVATITTTTILAPAAVAAGYNDLQLALL
                     TAAIGAGSMTLSHVNDSLFWVWTKFFNVPVSVGLRTWTFLTTIFGVIAFAVVGIIWML
                     FV"
     gene            5144..6484
                     /locus_tag="PXB27_001323"
     CDS             5144..6484
                     /locus_tag="PXB27_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HDL5346110.1"
                     /translation="MLKKTLLFTLLLSHSLYAEDMIQENKSANITLLAEITAQGQKIN
                     GVALEYPDNVLSGSDLRQLYQVKTALDSAEKENRSVLKAYVNNEPKVTALPQAGKFVI
                     IELDTADKNAATYSVKDANKAPMKFKSKDENGNIVETEKVQAVKIPEFFANRLIYSVE
                     QKGLLKLTSGTTLGKTEWTQTAEPDKIKTPYLDDFVAKQVSLDQSGNKLNYRLYSPLQ
                     KNGEKYPLVIFLHGSGQVGSDNLAHLLSSKGAIATLPYAPSFVLAPQYDQVFDPFDNA
                     AKGQKGGIHWQTENRINLVLKMIDETLKANPQIDTTRIYINGLSRGAEGGLNLLLARP
                     NFFAGALLMSGREAYTNEWVDGNATKTNLAAIKHIPIWFFHSKEDKVSPVKGSRINYR
                     ILKDELNAPNVKYTEFTFEQAGDNGIVNNNPHNTWDAVYNSPEVIGWLLQQKRH"
     gene            6496..7185
                     /locus_tag="PXB27_001324"
     CDS             6496..7185
                     /locus_tag="PXB27_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005577085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HDL5346111.1"
                     /translation="MFMYLSHPLDPKDLAWPGEPVVEVTRCTDVGEETPFSSFITKLP
                     NHCGTHMDAPRHFVKDGLSINELPIEYFCHKEVALIEIPKGEAEGITKEDLLPYVDIL
                     SKVSFAFLRTGFEKYRTENPAIYQNECPYISTGAGQYLSDTFPNLKGVGIDFLALGSP
                     SSRVPDAENPKNCHRAILGYYTGRFTTVIEDMHLAELPKNANIKQFFNAPWRIKGLDS
                     SQVTCIVELQQ"
     gene            7342..9159
                     /locus_tag="PXB27_001325"
     CDS             7342..9159
                     /locus_tag="PXB27_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HDL5346112.1"
                     /translation="MIIKDTHWTSQQASPNAFPERQALMPIWGGKNIERNQWQSLWLE
                     QAPNALLQDGLAYFHIPFCASHCIFCGFYRNAWKENYSQIYTDRVISELRYESSLWRG
                     QGKIHAVYFGGGTPTALHTEDLVRLIRACYRYLPLAEDCEFTIEGRISHFDIDKAKAC
                     IEAGVNRISIGIQTFNSEIRKRLGRKHSGEEAQTYLKALSELNAVIVADLIFGLPNQT
                     DDIWQNDIEIATSLPISGLDIYAFNNYPFLPINRLIEHGKLPAPAGFDTQSLHYAYAV
                     EKLKAQGWQQVSNNHFAFPNRGERNRYNTLVKSNMPCLAFGSGAGGNFGGLSYQVQSD
                     LKTYLDSPLDQKSLSFLSKHGEHKALLGVIQHSMELGYLDTTPFEQNAKAEKLLQQWE
                     ALDLLKREKNQVYLNTSGRYWSPTLIRKLMLTLPQSSKDNNMITPLTKELENKLRTNL
                     KENPGQILEMLAGMNQCSLESVINCLPKEMVMQTGGERFVEIMQAIAGWNEAVTFIIH
                     TPDAIAEVTAKLPQGKIGRGFYNFEHGVDGGIHGHLRYENCAAIYLLDRPFMGKRTVA
                     LTFINQSGEAMFKIFVGRDEQGQLRENQIQAMRKLIGGC"
     gene            9162..9779
                     /locus_tag="PXB27_001326"
     CDS             9162..9779
                     /locus_tag="PXB27_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL5346113.1"
                     /translation="MILVCGANGLLGRAICDKLPREKTIAVLRKDAMDTFFSERNIAV
                     EIADLLDLESCNALLQKTQPEILITVVGGKNEQGIRSDKTANINLIKAVEKYSPTTKV
                     VLVTSVGCAEQWDLISPLAQQALGEALKAKTEAEAYLQQTALNWLIIRPGGLNNDEVN
                     DEYQIVRDLPNERKMYVSRKSVATGIVRLLDSTENHQIFSIINRG"
     gene            complement(9863..10474)
                     /locus_tag="PXB27_001327"
     CDS             complement(9863..10474)
                     /locus_tag="PXB27_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentahemic C cytochrome"
                     /protein_id="HDL5346114.1"
                     /translation="MKSLSKIAFCFLAFIMPTFCFAGSNSYTTETIPLFADENLTQKI
                     GQLEAGAEIKLVQTTAKAAEIELNLWRKSKGFGRIWYHSFSKNITDAVFEKSFISNNP
                     LLEIVESKEDPNTGLVWQKVKMNVWVENAEFTQDLTNFWANTKNTYKTECSVCHKQRD
                     PKMHDANEWIAVFNGMVGFTDMDEATQKKVLRYLQMNAASSSQ"
     gene            10669..11577
                     /gene="glyQ"
                     /locus_tag="PXB27_001328"
     CDS             10669..11577
                     /gene="glyQ"
                     /locus_tag="PXB27_001328"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017806840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDL5346115.1"
                     /translation="MSTKFNVKTFQGMILALQDYWASKGCTIVQPFDLEVGAGTSHPM
                     TCLRALGPEPMAFAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLDS
                     LKMLGFDPTQNDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPV
                     TGEITYGLERLAMYIQGVDSVYDLVWSDGELGKTTYGDVFHQNEVEQSTYNFEYADVD
                     FLFQCFEQYEKEAQYLLGLEKPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILR
                     IRALTKGVAEAYYASREALGFPGCKK"
     gene            11657..11917
                     /locus_tag="PXB27_001329"
     CDS             11657..11917
                     /locus_tag="PXB27_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346116.1"
                     /translation="MKPILYFADWCPDTAPFVAELDRLGIEYEPCDITKGGSTLKPFL
                     KLRDTHSAFDHAKANGYIGIPALLIEGEKVVLDLAELEGIFG"
     gene            11960..12994
                     /locus_tag="PXB27_001330"
                     /pseudo
     CDS             11960..12994
                     /locus_tag="PXB27_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250793.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
BASE COUNT         3927 a         2590 c         2972 g         3717 t
ORIGIN      
        1 aatcacaaaa attcacattt attgttaata tttgtgatct tcatcacaga ttttatcttt
       61 tctattggtt aatataccgc catcaagttc attaatttat ttgagggaat aaacaatgag
      121 tcaaaaatat agcgtagccg tcatcggctt aggagcaatg ggtatgggag cagcaaaatc
      181 ttgtatcgct gccggattag acacatacgg tgtggatctt aacccgaatt atctggaaac
      241 cttaaaagca gcaggtgcta agaaagtcgg cacaagtgcg gtcgattttg ccgctgaatt
      301 agatgcggtg ttgttgttgg tggttaatgc caaccaagtg aatgcggtgt tattcggtga
      361 aaacggctta gcccaacatt tgaaacaagg tacggtggtg atggtatctt ccaccatttc
      421 tgcacaagat gcaaaaaata tttcacagaa gttaacagat ttaggcttgg tgatgttaga
      481 tgctccggtt tcgggtgggg cagcaaaagc agcacaaggt gaaatgactg tgatggcatc
      541 aggttccagc gaggcattcg agaaattaaa accggtatta gatgcggttg caggcaaagt
      601 gtataacatt ggcgaagaag tcggcttggg tgcaacggta aaaattatcc atcaattact
      661 tgccggtgtg catattgccg caggtgcaga agcaatggca ttggcggctc gtgccggtat
      721 tccgcttgat ttaatgtatg acgttgtaac caatgcggca ggcaattctt ggatgtttga
      781 aaaccgaatg aaacacgttg ttgagggcga ttacacccca ctctctatgg tggatatttt
      841 tgtgaaagat ttgggattgg tgaacgacac cgcaaaatct ttgcgtttcc cgcttcactt
      901 agccagcacc gcttattcta tgtttaccga agccagcaac gcaggttacg gcaaggaaga
      961 tgatagtgcg gtcattaaaa ttttcagtgg tatcgaatta cctaaaaaag aggggaaata
     1021 agatgttagg cattattgca gacgatttca ccggtgcgag tgacatcgcc agtttcttaa
     1081 ccgaaaacgg gctaaaaacg gtgcaaatga atggtgtgcc gaaaactaca cttaacagtc
     1141 aagttgatgc gattgtgatt agcctgaaat ctcgctctaa ccctgtggaa gaagcaatcc
     1201 aacaatcatt acaagcctta gattggctga aacaaaatgg ttgctcgcaa ttctacttta
     1261 aatattgttc cacttttgac agcacggaga aaggcaatat cgggccggta accgatgccc
     1321 taatggcggc attagatacc gattttaccg tagtcacacc ggctctgcct gtcaatggca
     1381 gaaccatttt caacggctat ttgtttgtgg gcgatgtgct actgaatgaa tctggtatgc
     1441 gtaaccaccc gattaccccg atgaaagatg ccaacttggt gcgtttaatg gatgcacaat
     1501 ctcaaggcaa aaccggcttg gttgcctatg ctgatgtgat tcaaggggca gagcgtgtga
     1561 aagcccgttt tgctgaattg aaacagcaag gctacaaata tgcggtagta gatgctgccg
     1621 ataattcaca acttgcggtg cttgccgaag cggttgccga ttttcaatta gtcacaggcg
     1681 gttcaggctt aggggcttat atggcggctt accgttcagg cggcaaaaaa ggcacgaatg
     1741 cgtttgtacc aagcaaaggc aaaaccgttg tgctttcggg ttcttgctcg gtaatgacca
     1801 atacccaagt ggcggcttac cagcaaaaag ccccaagtta tttcttagaa gcggaaaaag
     1861 ccattcagga tccgaactat gtagaaacgc tttatcaatg ggtgattgca aacctagacg
     1921 gtgactttgc cccaatggtt tatgccaccg ttccccctga aaaattgaaa gagattcaaa
     1981 cccaattcgg cggcgataaa gccagccacg ccattgaaaa tacctttgcc aaattagccg
     2041 aaaaattaca agccgcaggc gtaactaact ttattaccgc aggcggtgaa acttcgagca
     2101 ttgtggttca gcaacttgga ttcagcggtt tccacatcgg caaacaaatt gcaccgggcg
     2161 tgccgtggtt acaagcggtc gaaaaaccga tttttcttgc attgaaatcg ggtaacttcg
     2221 gtaaagaaga tttcttcagt tttgctcagg agatgtttgt atgacagaac aagaacaaaa
     2281 gattgaaatg gtcaatcttg cccgctcgct gtttgagcgg ggctataccg ttggcggtgc
     2341 gggcaacctc tccgtgcgtt tagatgaaaa tcgcatttta gttaccccaa ccggctcttc
     2401 gctcgggcgt ttagaggcag accgcctttc cgtattggat atgcagggta atgttctttc
     2461 aggagataaa ccttcaaaag aatcggtatt ccacttggcg atgtatcaga aaaatccggc
     2521 ttgccaagca atagtgcatc tgcattccac ttatttaacc gcactttctt gcttggaaaa
     2581 tttggatacc gacaacgcta tgcgtgcttt caccccttac tatgtgatgc gggtaggaaa
     2641 attgccggtc attccttact accgtccggg cgacatcaac attgcccgag agctggggga
     2701 acgagcctta gacggcaaag cctttttact ggcaaatcac ggggtggtag tcacaggttc
     2761 agacttgcgt gatgcggtgg ataacacaga agagttggaa gaaacctcca agctctattt
     2821 tattctacaa ggtcaaaaag tccgttatct caccgatgac gaagtcaatg atctgaaaaa
     2881 cagaggaaaa taaaatgcct aaatttgcag caaaccttac gatgatgttt aacgaagtgc
     2941 cgtttttaga tcgttttgaa gcggctgcca acgctggatt taaatatgtc gagtatttat
     3001 ggccttatga ttatccggca gcagaattga aagccaaact tgagcaatat ggcttaaaac
     3061 aggtgttgtt caataccgca gccggagatg ttgctcaagg tgaatggggt gtttctgcca
     3121 ttccggggcg tgaagctgat agccatcgtg atattgattt agcgttggaa tatgccctcg
     3181 ccttaggttg tgagaatgtt cacgttatgg cagcggtcgt gccagaaggc gaagatcgtg
     3241 aagcctataa acaaaccttt atcaaaaacg tgcaatatgc cgccgataaa ttcaaaccgc
     3301 acggcattaa cgtcttgctt gaagccctaa gccctgaagt gaaaccgaat tatctgctta
     3361 aaagccagtt tgatacgcta gaaattgtgg aagcggtaaa ccgagacaat gtgtttgtac
     3421 agcttgacta cttccacgca cagaatgtgg acggcaactt atctcgccta accgacaaac
     3481 tcaagggtaa atttgcccac attcaaattg cctctgtgcc agatcgccac gaaccggacg
     3541 aaggggaaat taactaccaa tatatcttca acaaactcga tgaaatcggg tatgaaggct
     3601 atgtaggttg cgagtataaa ccacgagcgg aaaccacctc aggactaggt tggtttgagc
     3661 agtataaata accacgatga ttagcggata aatacgtgaa tacaacattt agaaggaaac
     3721 tttatgtcaa atgaaatgtt aattttgatt ggattagcaa gcgtagttgc tcttctggtc
     3781 attatgatta aaggtaaagt acacccattc ttagcattgg ggttagtgag tatcgcagtt
     3841 gccttaactg caggtattcc aatgagtaaa gtgatcccaa ccttgatttc aggtatggga
     3901 agtactttag gtagcgttgc actcatagta ggtttgggag caatgttagg gaaaatcatt
     3961 gaaaaatctc acggtgcgga tgtattagca aattggttgt tacagcgttt tggtgagaaa
     4021 aacgctccct ttgcattagg tatgacaggt tttatattcg gtattcctgt tttcgttgat
     4081 gttggtttta tcgtattgct cccgattatt tttagtgtag cgagacgttt agggggaaat
     4141 atccttgttt attctttgcc aatcgggctt gcgatgttac ttgttcacgt tttagttcca
     4201 cctcatccag gtattgtagc gggtgctcaa aacttaaacg cagatattgg ctttgtattg
     4261 ggaattggct tagtagcagc gctacctgca tatttaatcg gtcaaaaatt tattccgttc
     4321 tttacaaaaa ataatttcgt aaaagtagta acttcaaccg atcttttaca gcaccaaaaa
     4381 gatttgatta aagagaaagg cattgaacta ccttcttttg gtaaagtgtt agcaatgatt
     4441 tctttcccgc taatcctgat tatgttaggt acagtaacgg caacaacctt gccgaaagaa
     4501 catatcgtgc gtgaagtatt cagtatggta ggaagctctc cgtttgcctt atttgtggct
     4561 gtttgtcttt ctgcttatgt attaggtttt agatacggtt ggactaagca taaattagaa
     4621 gaagtactgg agtctgcgtt agcaccgatt gcggggatta ttttaatcac cggtgcaggc
     4681 ggtatgttcg gtaaagtgtt agatgccagt ggcgtaggta aagcattagc agatgtatta
     4741 gccaacacgg gcttaccgtt attggttctt tctttcatac tttctgcatt attgcgtgca
     4801 gcacaaggtt ctgcaacggt ggcaactatt actactacaa ctattctagc tcctgcagct
     4861 gtagcagcag gttataatga tctgcaatta gcacttctta ctgcagctat tggtgcaggt
     4921 tctatgacat tatcccacgt gaatgattca ctcttctggg tatggacgaa attcttcaat
     4981 gttcctgtat ccgtaggctt aagaacttgg acgtttttaa cgactatttt cggtgtaata
     5041 gcctttgccg ttgttggcat tatttggatg ttattcgttt aatatcactc tgttactgct
     5101 taaaatccac ctcttatgag gtggattttt ttaggagaaa cctatgctga aaaaaacact
     5161 tttatttacg ctattacttt ctcactccct ctatgcagag gatatgattc aggaaaataa
     5221 atcagcgaat attacgttgc ttgccgaaat caccgctcaa ggtcaaaaaa ttaacggcgt
     5281 ggcattggaa tatcctgata atgtcttatc aggcagcgat cttcgccaac tgtatcaggt
     5341 caaaacggca ttagatagtg cggagaaaga aaaccgttcg gtgctcaaag cctatgtcaa
     5401 taatgagcca aaagtgaccg cacttccgca agccggtaaa tttgtgatta ttgaattgga
     5461 tacggcagat aaaaatgcgg caacttatag cgtaaaagat gctaataaag cgccgatgaa
     5521 atttaaatcc aaagatgaaa acggcaacat tgtagaaaca gagaaagtac aagcggtcaa
     5581 aattccagag ttttttgcaa atcgcctaat ctattcggtg gaacaaaaag gcttgcttaa
     5641 attaaccagc ggcacaactc taggcaagac tgaatggaca caaaccgctg aacccgacaa
     5701 aatcaaaacc ccttatctcg atgattttgt cgcaaaacaa gtgagcttag accagtctgg
     5761 aaataaactg aactaccgtc tctattcgcc tttgcaaaaa aatggtgaaa aatacccgct
     5821 tgtgattttc ctacacggtt cagggcaagt cggttcggat aaccttgctc acctgctttc
     5881 cagtaaaggg gcgattgcca ccctgcctta tgccccaagt tttgtacttg ctccgcaata
     5941 cgaccaagta ttcgacccat ttgataatgc cgccaaaggg caaaaaggcg gtattcactg
     6001 gcaaacggaa aatcgtatca atctcgtgtt aaaaatgatt gatgaaaccc taaaagccaa
     6061 tcctcaaatt gataccacac gcatttatat caatggctta tctcgaggtg ctgaaggggg
     6121 cttaaatctg ttgttggctc gcccgaattt ctttgccgga gcattattaa tgagcggtcg
     6181 tgaagcctac accaacgaat gggtggacgg caatgccacc aaaacgaatt tggcggcaat
     6241 taaacatata ccgatttggt ttttccatag taaagaggac aaagtctcgc cggtaaaagg
     6301 ttctcgcatt aactaccgca ttctcaaaga tgagttaaat gcccccaatg tgaaatatac
     6361 cgaattcact tttgaacaag ccggcgacaa cggcattgtc aataataacc ctcataatac
     6421 ttgggacgct gtgtataaca gccctgaggt gattgggtgg ttacttcagc aaaaacgcca
     6481 ctaaggaggc tcactgtgtt tatgtatttg tctcacccgt tagatcctaa agatttagct
     6541 tggccgggag aacctgttgt tgaggtgact cgctgcacag acgtaggtga agaaacgcca
     6601 tttagcagtt ttatcacgaa actaccgaat cattgcggca cacatatgga cgctcctcgc
     6661 cactttgtga aagacggttt aagtatcaat gaattgccga tagagtattt ctgccataaa
     6721 gaggtcgctt taattgaaat tcccaaagga gaggcagaag ggatcaccaa agaagattta
     6781 ttgccttatg tcgatattct ctccaaggtg tcttttgcct ttttacgaac ggggtttgag
     6841 aaatatagaa ccgagaaccc tgctatttat caaaatgaat gcccctatat ttcaaccggt
     6901 gccgggcagt atttatctga tactttcccg aatttgaaag gagtaggcat tgatttcttg
     6961 gcactagggt ctccttcttc ccgagtgcct gatgcggaaa accctaaaaa ttgccatcga
     7021 gcgattttag gctactatac cggcaggttt accactgtga ttgaagatat gcacttagcg
     7081 gaactcccta aaaatgccaa cattaagcag ttttttaatg caccgtggcg aattaaaggg
     7141 ttagattcca gtcaagtgac ttgtattgtg gaattacagc aataaaccac agatatattc
     7201 attttaggag aggctattta ctatagtaag cataactcaa tcattaattg aaaataatta
     7261 ttatttgatt ttaatcaagt cattcataac ttaattttgt tataaatatg accttgttta
     7321 tagcactatt ttgtttcgat aatgattata aaagatacac actggacatc tcaacaagcc
     7381 tctccaaatg ctttcccaga acgacaggca cttatgccta tttggggtgg gaaaaatatt
     7441 gaacgcaatc aatggcaatc tttatggctg gaacaagcac caaatgcgtt attacaggat
     7501 ggtttagctt atttccatat tccgttttgc gctagtcatt gtattttttg tggtttttat
     7561 cgcaatgctt ggaaggaaaa ttatagccag atttatactg atcgtgtgat tagtgaactt
     7621 cgatatgaat cttcactatg gcgaggacaa ggtaagattc atgccgttta ttttggtgga
     7681 ggaacgccaa cagcgttaca tacagaagat ttagtacgat tgattcgagc ttgttatcgt
     7741 tatttgccac tcgctgaaga ctgtgaattt actattgagg gtagaattag ccattttgat
     7801 atagataagg ctaaggcttg tattgaagca ggcgtgaacc gtatttctat tggtattcag
     7861 acttttaact ccgaaattcg caaacgttta gggcgcaaac atagtggcga agaggctcaa
     7921 acttatttaa aagccttatc ggaattaaat gctgtgattg ttgctgattt aatttttggc
     7981 ttgccaaacc aaacagatga tatctggcaa aacgatattg aaattgcgac tagtttaccg
     8041 atctcaggct tagatattta cgcctttaat aattatcctt tcctgcctat taatcgctta
     8101 attgaacatg gaaaattacc tgcgcccgca gggtttgaca cccaaagctt acactatgct
     8161 tatgcagtag agaagttaaa agcacaaggt tggcagcaag tgagcaataa ccactttgct
     8221 tttccaaatc gaggtgaacg caatcgctat aatactttgg ttaaatcgaa tatgccttgt
     8281 ttggcatttg gctccggtgc ggggggaaat tttggtgggc tgagttacca agtacaatct
     8341 gatttaaaga cctacttaga ttcgcctctt gatcagaaaa gcctttcttt tttgagtaaa
     8401 catggtgaac ataaagcact gcttggtgtg atacaacata gcatggaatt gggttactta
     8461 gatacaacgc cttttgaaca aaatgcgaaa gctgaaaaat tattgcaaca atgggaagcg
     8521 cttgatttac tgaagcgtga aaaaaatcaa gtatatctca ataccagtgg tcgttactgg
     8581 tcaccaacat taattcgtaa actaatgtta acacttcctc aatcaagtaa ggataacaac
     8641 atgataacgc cattaactaa agaattagaa aataaattgc ggacaaattt aaaagaaaac
     8701 ccagggcaaa ttttagaaat gctggcagga atgaatcaat gtagcttgga atcggtaatt
     8761 aactgtctgc ccaaagaaat ggtaatgcaa acaggtggtg agcgttttgt ggaaattatg
     8821 caggcaattg ctggttggaa tgaagctgtg acttttatta ttcatacgcc tgatgcgatt
     8881 gcagaggtaa ctgctaaatt gccacaaggt aaaattgggc gaggttttta taattttgaa
     8941 catggtgtag atggaggaat tcacggtcat ttacgctatg aaaattgtgc tgctatttac
     9001 ttacttgatc gcccatttat gggaaaaaga acggtggcgt taacttttat caatcaaagc
     9061 ggtgaggcga tgtttaagat ttttgttggg cgtgatgaac aaggtcaatt acgagaaaat
     9121 cagattcaag ccatgcggaa attaattgga ggatgctagt catgattttg gtttgtgggg
     9181 caaatgggtt gttaggacga gcaatctgtg acaaattacc tagagagaaa acgatagccg
     9241 tcttacgaaa agatgctatg gatacgtttt tcagcgaacg aaatatagca gtagaaatag
     9301 cagatttatt agatttggaa tcctgcaacg ccttgttaca aaaaacacaa ccggaaatct
     9361 tgattacggt agtaggagga aaaaatgaac aaggtattcg tagcgacaaa acagcaaata
     9421 ttaatttgat aaaagcagtt gaaaaatatt caccaactac gaaagtagtt ttagttacta
     9481 gtgttgggtg tgcagaacaa tgggatttaa ttagcccatt agcacagcaa gctttaggtg
     9541 aagcactaaa agccaagacg gaagctgaag cttatttaca gcaaacagca ttaaattggc
     9601 tgattattcg acccggtggg ttaaataatg atgaagtaaa tgatgagtat cagattgtgc
     9661 gtgatttacc taatgaacgg aaaatgtatg taagccgtaa atctgtcgca acaggcatag
     9721 tgcgtttact ggatagtacg gaaaatcatc aaatcttctc tattattaat aggggatagt
     9781 atatttcaac gaaggcaagc gggcatattt tatggttatt ttgcaactat aaaacagacc
     9841 gcttgtgaga tctttttcta ttttattgag aactggatgc cgcattcatc tgcaagtagc
     9901 gaagcacctt tttctgggta gcttcgtcca tatcggtaaa accgaccatt ccgttaaata
     9961 ctgcaatcca ttcgtttgca tcatgcattt taggatcacg ttgtttatgg cacacgctgc
    10021 attctgtttt ataggtattt ttggtgttcg cccaaaagtt agtgagatct tgggtaaatt
    10081 cggcattttc cacccacacg ttcattttca ctttttgcca taccaagccg gtgttcggat
    10141 cttccttgct ctctacaatc tctaacagtg ggttgttgct gataaaagat ttctcaaata
    10201 ccgcatcggt aatattttta ctgaaagaat gataccaaat ccgcccgaaa cctttgcttt
    10261 ttcgccataa attcagctca atttcagcgg cttttgcggt tgtttgcacc aatttaattt
    10321 ctgctccggc ttctagctgc ccgattttct gtgtcaaatt ttcatcggca aataaaggga
    10381 ttgtttccgt ggtgtaactg tttgaaccgg caaaacaaaa tgttggcatg atgaatgcca
    10441 agaaacagaa agcgattttc gataatgatt tcatcataaa ccccacaaat tagatgagaa
    10501 attaggttat atgttacaca aattgagcaa aaaagcgttg atttacatca taaaactgat
    10561 tttatgtcga tttcttaatt ttttgcaaaa aaatcccaaa aaatgactgc ttacaagcta
    10621 taataaccag tatttttcga ccatttttca aattcaagag aaaataaaat gagtacaaaa
    10681 ttcaacgtaa aaacctttca aggaatgatt ttagccctgc aagactactg ggcaagcaaa
    10741 ggttgcacca ttgtgcagcc gttcgattta gaagtggggg caggcacctc gcacccaatg
    10801 acctgcctgc gtgcgcttgg ccctgagcca atggcgtttg cctatgtgca accatctcgc
    10861 cgcccgacag acggtcgcta cggcgaaaat ccgaaccgtt tacagcacta ctaccagttc
    10921 caagtggtga ttaaaccttc gccggataac attcaagaac tttacctcga cagcctgaaa
    10981 atgctcggtt tcgacccaac ccaaaacgac attcgttttg tggaagacaa ctgggaaaac
    11041 ccaacgcttg gggcgtgggg cttaggttgg gaagtgtggc tgaacggaat ggaagtgacc
    11101 caattcacct acttccagca agtcggcggc ttggaatgta aaccggtgac aggcgaaatc
    11161 acctacggct tagagcgttt ggcgatgtac attcaaggcg tggacagcgt gtacgacctc
    11221 gtgtggtctg acggcgaact cggtaaaacc acctacggcg acgtattcca ccaaaacgaa
    11281 gtggagcaat ccacctacaa ctttgaatac gctgatgtgg acttcctgtt ccaatgcttc
    11341 gagcagtacg aaaaagaagc ccaatacctg ttaggcctag aaaaaccatt gccattgccg
    11401 gcatacgaac gcattttaaa agccgctcac agctttaact tattagatgc tcgtaaagcg
    11461 atctcggtaa ctgaacgcca acgctacatt ctacgcattc gtgccttaac caaaggcgtt
    11521 gctgaagcgt actatgccag ccgtgaagcg ttggggttcc cggggtgtaa aaaataatcg
    11581 tagggacgcc acgcttggcg tccgtcaata aattacaaaa cggacacgaa gcatcgtgtc
    11641 cctacaagga tttacgatga aaccgatttt atacttcgcc gattggtgtc cggataccgc
    11701 cccatttgtg gcagaactag accgcttggg cattgaatat gagccgtgcg acatcaccaa
    11761 aggaggctca acacttaaac catttttgaa actgcgagac acgcattctg cctttgacca
    11821 cgccaaagcc aacggctata ttggcattcc ggcattgctg atcgaaggcg aaaaagtcgt
    11881 gctggatttg gctgaattag agggtatttt cggctaacaa gcggttgaat ttggcggaaa
    11941 tgttgcaaag gagccaataa tgaagacagt aatagcagat aattcggctg aatttgtgaa
    12001 tgaaatttca caacttattc aatcgagtaa gcaacgaatg accgttgcgg ttaatgcaga
    12061 attaaccttg ctttattggc atatcggaaa acggatcaat gactatattt tgcagggtga
    12121 acgtgcggaa tatgggcaag aagtggtgaa aaatcttgct caaagtctga cggagcaatt
    12181 tggtaaaggt tggagtaaac gccatttaaa ttatacaatg caatttgcta caactttccc
    12241 taatttggaa attgtgcacg cactgcgtgc acaattaagc tggacacatt ttaagcagat
    12301 tattcatatt gatgatccaa caaagcgagg gttctatgca acaatggctg cacaggagcg
    12361 ttggagtaca aggacattag aagatcgcat tggctcatta ttgtttgaac gcacggcaat
    12421 ttccaaaaag cccgatgaaa ccattattgc tgaattgagt ttgctacgtg aaaaaggcga
    12481 atataatcag tcaatggtgt tgaaagatcc ctatattttg gattttctcg accttaacga
    12541 ccgttatatg gaacgtgatc tcgaagatgc gattctacga gagatcgaac agtttttact
    12601 ggaacttggg gcgggtttta cctttgtcgc ccgccaaaaa aggattcaga ttgataatga
    12661 tgatttctat atcgatctgc tgttttataa ccgtaaactc aaacgcctga ttgcgattga
    12721 actaaaaacc gaaagcttca aacattccca caaaagttaa atggaactct atttggcgtg
    12781 gctggcgaaa tacgagcaag aagcagacga aaacccaccg cttggaatga ttctctgcac
    12841 cggtaaaaag caggagcaag tggaattgtt ggatttgaaa aattccgata ttcatattgc
    12901 ggaatatatg accgtgttgc cgagtaaagc ggtattggaa caacggttgc atttggcggt
    12961 ggaaagggcg aaagagcggt ttttactaaa atagcaagcg gttaaaatca gcggaaatgt
    13021 tgcaaaatga aagtatgatg gaaacgattt ggttgcatat tccgttggtg ggtggttaga
    13081 gattttcaaa tctcccccct ccccctcttt ggctaaagag ggggcgatca tttagtgagt
    13141 aattttaatt catctgacta tttacaatct taatgaagaa agatgaatta taaatttacg
    13201 aagatc
//
LOCUS       DANLUQ010000060        12892 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.060, whole genome shotgun sequence.
ACCESSION   DANLUQ010000060 DANLUQ010000000
VERSION     DANLUQ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12892
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.060"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(271..1404)
                     /locus_tag="PXB27_001331"
     CDS             complement(271..1404)
                     /locus_tag="PXB27_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis PLP-dependent protein"
                     /protein_id="HDL5346117.1"
                     /translation="MTKFNNIETTLVQIGNRTDPRTGAVAMPIILSTAYGRDGLGEST
                     GWDYTRTKNPTRAVLEQGIADLEGGDAGFAMASGMAAIQLVMSLFKAPDEWIISSDVY
                     GGSYRLFDFSHKNHGTLKPVYVNTADLAAIEAAITPNTKAIFVETPSNPLMEECDVAA
                     IAKIAKKHNLMLIVDNTFLTPALFRPIEHGADIVIHSATKYLSGHNDVLAGLIVAKDS
                     DATKVNGQNLTERLFYFQNCAGAVLSPFDSYLAVRGMKTLALRMERHQSNATELAKFL
                     AEQPEIDSVLYSGKSGMLSFRLQKEEWVAKFLKAIKLITFAESLGGAESFITYPATQT
                     HMDIPEAERITRGITNNLLRFSVGLEHVEDIKADLLQAFKQLK"
     gene            complement(1421..2233)
                     /gene="dapB"
                     /locus_tag="PXB27_001332"
     CDS             complement(1421..2233)
                     /gene="dapB"
                     /locus_tag="PXB27_001332"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005696006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDL5346118.1"
                     /translation="MTLKIGIVGAGGRMGRQLIQAVHNAQGVELGSAFERKGSSLVGS
                     DAGEVAGVGTLGVVISDDVAQNVAGFDLLIDFTRPEGTLEHLDICVAHHKQMVIGTTG
                     FDDAGKAAIKAASEKIGIVFASNYSVGVNLVFKLLEKAAKVMGDYCDIEVIEAHHRHK
                     VDAPSGTALSMGEHIAKTLGRDLKTHGVFERNGITGERKRDEIGFATIRAGDVVGEHS
                     VWFADEGERVEIAHKASSRMTFANGAVRAAKWLNGKQNGLFDMTDVLDLNNL"
     gene            complement(2284..2661)
                     /locus_tag="PXB27_001333"
     CDS             complement(2284..2661)
                     /locus_tag="PXB27_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346119.1"
                     /translation="MGIKVLFGTKISAELQYLPEKDLVKILQFKQHVEIYGLENLAGR
                     NKSSDDVPTNDPYWAEKVAKAQKYSLWHYHIGIPEYDTSQGFGNYTSEYILHYVKGEN
                     FIQIVDFTSHPPFKLPSESYLQN"
     gene            complement(2651..3124)
                     /locus_tag="PXB27_001334"
     CDS             complement(2651..3124)
                     /locus_tag="PXB27_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346120.1"
                     /translation="MMKNLPLSMADTLAVIYPAFDFAADDKPATEQQISLLNFRITMA
                     NQLLIACIETIEKNPNFWAEIDLNDFKKYLNQNKVVIFDTQQYFTDGSRTMAILDNMK
                     SNIRKIEQQLSQATAFNFDLERMKERLNDTFHEVPKDITNVNEFRNWLKGVANGH"
     gene            complement(3192..4280)
                     /gene="rlmM"
                     /locus_tag="PXB27_001335"
     CDS             complement(3192..4280)
                     /gene="rlmM"
                     /locus_tag="PXB27_001335"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HDL5346121.1"
                     /translation="MFNKLALYCRAGFEKEVAGEITDKAAQQGIFGFANLKENSGYVI
                     FECYQAGEADRLAQALKFEQLIFVRQMLVVGDLLQNLSENDRISPILAQYAELNPRQS
                     SDVFVETPDTNEAKELLTFCRKFTVPLRSALKKQGWLGKSENMQGSVSLHILFTRPGA
                     CYVGYAYNHNKSPFFMGIPRLKFPAEAPSRSTLKLEEAILTFIPQAEEKKRFNDTMVG
                     VDLGACPGGWTYQLVKRGVFVYAVDHGKMAASLHETGRIEHCPEDGFKFQPPKRKQID
                     WLVCDMVEQPIRISKLIAKWLTNGWCRETIFNLKLPMKKRYQEVQLCLNFLEEELTKH
                     GFWFKMQAKHLYHDREEITVHIAVMGQK"
     gene            complement(4280..4672)
                     /locus_tag="PXB27_001336"
     CDS             complement(4280..4672)
                     /locus_tag="PXB27_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HDL5346122.1"
                     /translation="MKNKFVAIAAFSGLFTVAFGAIASHALSAQLSPKALGWIETGIK
                     YQMFHTLAILALGLFQIANFGQNPPVCRAKAFNIIGSNWALGILLFSGNLYLLALNVP
                     TIHWLTPIGGLCFMLGWIALIYISVRKA"
     gene            complement(4779..6180)
                     /locus_tag="PXB27_001337"
                     /pseudo
     CDS             complement(4779..6180)
                     /locus_tag="PXB27_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
     gene            complement(6335..8692)
                     /locus_tag="PXB27_001338"
     CDS             complement(6335..8692)
                     /locus_tag="PXB27_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HDL5346123.1"
                     /translation="MNTALEKGKFVFLDKPKNTITTAAHWGALNVTVENGKVVKSEGS
                     LAKNHQNELQDVVADQLYSPVRVKYPMVRKGYLEGNKDTTLRGRDKWVRISWDKAFEL
                     VSGEIQRVREQYGSESIFGGSYGWYSSGSLHASRTLLQRYLNVTGGFTNTKGDYSTGA
                     VQVIMPYVLGNIEVYAQQTSWHTIVESTELLVIWSSNPLNTLRIAWTSAEPEGIDYLA
                     KLKASGKRIICIDPVKSETCQFLEAEWLPINTGTDVALILGIAHTLITENKHDHDFLT
                     KYTVGFEQFADYVLGKADNLPKTAEWASQICDIPEEVIKTLANDFVTHRTMLMSGWGL
                     QRLQHGEQSHWALVTLAAMLGQIGLPGGGFGLNYRYSTNGLNVNSGVRLGAINAGKPN
                     PDMPFFPVSRIADALLKPNQPYDYNGQTLHYPNIKLVYWAGGNPFAHHPDTNYLVKAW
                     QQPETMIVNECYWTPTARMADIVLPATTSYERNDLTVAGDYVAKHIIPMKQVVEPQFE
                     AKNDYDIFAELAKRAGKEQAFTEGKSEMEWLKEFYQVAFEAGEKANTPLLDFEAFWAE
                     NKVLSFEAEHQNNTFVRYQDYRTDPIKHKLATPSGKIEIFSETIAKMNYEDCKGHPTW
                     FEHHEYSGKTNEAEPLALVTPHIKYRLHSQLAYSSLRQYYEVNGREPMLIHTKDAADR
                     GITSGDIVRIFNKRGQVLAGAVVTDGIIQGTVALYEGGWYDPQDLGKTDTPLCKNGNP
                     NVLTRNEGSSKLGQGNAPNTTTVQVEKYVGDAPEVTVFKAPFFSE"
     gene            complement(8828..11434)
                     /gene="acnB"
                     /locus_tag="PXB27_001339"
     CDS             complement(8828..11434)
                     /gene="acnB"
                     /locus_tag="PXB27_001339"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014391308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HDL5346124.1"
                     /translation="MENFIENYQQQVDERAALGVVPQPLSAEQTAQLVELLKNPPAEQ
                     AVFLLDLFTNRIPAGVDEAAYVKAAFLADITKGVATSPLISPAHAVKLLGTMQGGYNI
                     ETLLSALDNPELAPIAVEALSKTLLMFDNFHDVKERADQGNPFAQQVLESWANADWFT
                     SRPKLAEKLTVTVFKVTGETNTDDLSPAQDAWSRPDIPLHALAMLKNERDGIFPDENG
                     HTGPIKQLEALKEKGFPLAYVGDVVGTGSSRKSATNSVLWFMGEDIPYIPNKRAGGVV
                     LGGKIAPIFFNTLEDAGSLPIEVDVTNLNMGDVIDIYPYAGKICKHNSDEVLAEFELK
                     TEVLLDEVRAGGRIPLIIGRGLTHKARVALGLPESDVFIKPQSVSESNKGYTLAQKMV
                     GRACGVEGVRPGQYCEPRMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAA
                     YPKPVDVITHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIG
                     ISFPAGSGLVAFAAATGVMPLDMPESVLVRFSGTMQPGITLRDLVHAIPYYAIQQGLL
                     TVEKKGKKNIFSGRILEIEGLEDLKIEQAFELSDASAERSAAACTIKLNKEPIIEYLN
                     SNITLLKWMIAEGYDDKRTLERRIANMQAWLDNPELLEADENAEYAAVIEINLDEIKE
                     PIVCVPNDPDDARLLSQVQGDKIDEVFIGSCMTNIGHFRAAGKLLSQAQGELPTKLWI
                     APPTKMDAGLLTEEGYYSIYGKSGARVEMPGCSLCMGNQARVANNANVVSTSTRNFPN
                     RLGQGANVYLASAELAAVSALLGKLPTPQEYLDYMNNLQKDKDDIYRYMNFDQINSYV
                     KKADNVIVQVAV"
     gene            complement(11454..12701)
                     /gene="icd"
                     /locus_tag="PXB27_001340"
     CDS             complement(11454..12701)
                     /gene="icd"
                     /locus_tag="PXB27_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HDL5346125.1"
                     /translation="MQTQVQIPQGETIQLNQDGSLQVPDNPIIPFIEGDGIGVDVTPA
                     MKAVLDAAVEKAYQGKRKIAWMEIYAGEKANKLYGENTWLPDETLELIRQYHVAIKGP
                     LMTPVGGGIRSLNVAMRQGLDLYNCLRPIRYYDGTPSPVKHPELVDMVIFRENSEDIY
                     AGVEWVAGSPEANKVIEFLQQEMGVKKIRFTEDCGIGIKPVSKQGTQRLVRAALQYVI
                     DNNRKSLTLVHKGNIMKFTEGAFKEWGYQVAEEFGAKLIDKGPWMSLTNPKTGEEIII
                     KDSIADAFLQEILLHPTEYDVIATLNLNGDYISDALAAQVGGIGISPGANIGFEAAIF
                     EATHGTAPKIAGQNKGNPGSLILSGEMMLRHLGWVEAADLVVKAVSKTIANKTVTFDF
                     AEMLEGVTLRSTSEFAQDIIANM"
BASE COUNT         3684 a         2974 c         2469 g         3765 t
ORIGIN      
        1 tgtaatgtgt ggctaaaaaa tttgaacaaa cgaagcgaca gcgttagcaa aaacttaaaa
       61 tgatgtacgg atttaaaaat ctgttcgttt gcttgtttta taagcaaact aatttcgcat
      121 aattattatt atgttaaata gatagttaaa ataacacatt taattaaatc ttttcgtgtt
      181 tctctcctcc tttattaaaa aagaaaacgg tcagattggt aaaatctata cctaatatga
      241 ccgcttgtga atacaaaaaa attacttcaa ttacttcaac tgtttaaagg cttgcagtaa
      301 atcagcttta atatcttcaa cgtgttctaa accaacggaa aaacgcagta aattattcgt
      361 aatgcctcgt gtaatacgtt ctgcttccgg aatatccata tgggtttgtg ttgcaggata
      421 agtaataaaa ctttctgcac cgcctaaact ttccgcaaag gtaatcaatt taattgcctt
      481 gagaaatttc gctacccact cttctttttg taaacggaaa gataacatac cacttttgcc
      541 tgaatataac acgctgtcaa tttcaggttg ctctgctaag aatttagcca gttcggtggc
      601 attagactga tggcgttcca ttcgtaatgc caaggttttc ataccacgca cggcaaggta
      661 agaatcaaat ggcgacagca ctgcaccggc acagttttgg aaatagaata aacgctcggt
      721 caaattctgc ccgtttactt tggtagcatc agaatctttc gcaacaatta agccggctaa
      781 cacatcatta tgccccgaaa gatacttggt cgcactgtga atcacaatat cagctccgtg
      841 ctcaatcgga cggaataacg ctggggttaa gaacgtatta tcaacgatca acattaaatt
      901 gtgttttttc gcaattttag caatggcagc aacgtcgcac tcctccatca atggatttga
      961 cggcgtttcc acgaaaatcg ccttagtatt cggtgtaata gctgcttcaa tcgctgctaa
     1021 atccgctgta ttaacataaa ccggctttaa tgtgccgtga tttttgtggc taaaatcaaa
     1081 caagcggtaa gaaccaccat aaacatcgct ggaaataatc cattcatcag gtgctttaaa
     1141 taatgacatt actagctgaa tcgctgccat tccagatgcc atcgcaaaac cggcatcacc
     1201 gccttctaaa tccgcaatcc cctgctctaa tacagctcga gttgggtttt tggtacgggt
     1261 gtaatcccag ccggtagatt cgcctaagcc atcacgcccg taagcagttg ataagataat
     1321 cggcattgcc accgcccctg tacgaggatc agttctgtta ccgatttgca ctaatgtggt
     1381 ttcaatatta ttgaattttg tcattgttat tatccttcaa ttacagatta tttagatcca
     1441 atacgtctgt catatcaaac aagccatttt gtttgccatt taaccatttt gcagcacgca
     1501 ctgcgccatt ggcaaacgtc attcgactag aggctttatg tgcgatttcg acacgttcgc
     1561 cttcatcagc gaaccataca ctatgctccc ctacaacatc accggcacga atagtagcaa
     1621 agccgatttc atcacgtttg cgttcgcctg taataccgtt acgttcaaat acgccatgag
     1681 ttttcaagtc acgcccaaga gttttggcaa tatgttctcc cattgataaa gccgtacctg
     1741 atggtgcatc aactttatgg cgatggtgtg cctcaatcac ttcaatatca caataatcgc
     1801 ccattacttt tgctgctttt tcgagcagtt taaacaccag attcacccca acgctgtagt
     1861 ttgaggcaaa aacgataccg attttttcac tcgctgcttt gattgcagct ttacctgcat
     1921 catcaaaacc ggttgtgcca atcaccattt gtttatgatg agccacacaa atatccaaat
     1981 gttctagcgt gccttcaggg cgagtaaaat caattagtaa atcaaaacct gctacatttt
     2041 gggcgacatc atcagaaatc accacaccta atgtaccaac cccggctact tcaccagcat
     2101 cactacctac taatgatgaa cctttacgct caaaagcaga ccctaattct acgccttgtg
     2161 cattatggac ggcttgaatt aattgacgcc ccattcgccc accggcaccg acgattccaa
     2221 tttttaatgt catatttgtt cttccttttt atatttgttt tttacaagcg gttacatttt
     2281 taattaattt tgcaaatagc tttctgatgg caatttaaaa ggtgggtgag aagtaaaatc
     2341 aacaatttga ataaagtttt cacctttcac ataatgtaaa atatactcag aagtataatt
     2401 accaaatcct tgactggtat catattctgg aatgccgata tgataatgcc ataaagaata
     2461 tttttgtgct ttagccacct tctctgccca ataaggatca ttcgttggca catcatcaga
     2521 acttttattt cgaccagcta gattttccaa tccataaatt tcaacgtgtt gtttaaactg
     2581 caggattttg actagatctt tttcaggcaa atattgcaat tcggctgaaa tttttgtacc
     2641 aaataaaact ttaatgccca tttgcaacac ctttcaacca gtttctaaat tcattaacat
     2701 tcgttatatc tttaggcact tcgtgaaaag tatcatttaa ccgctctttc attcgctcta
     2761 agtcaaaatt aaatgcggtt gcttgagaga gttgctgttc aattttgcga atattggact
     2821 tcatattatc caatatagcc attgtccttg agccatcagt aaaatactgt tgggtatcga
     2881 aaatcaccac cttattttga tttaggtatt ttttgaaatc gtttaaatca atttcagccc
     2941 aaaagtttgg atttttttcg atagtttcaa tacaggcaat taataattga tttgccattg
     3001 taatacgaaa atttaacaat gagatttgct gctctgttgc aggtttatca tcagctgcga
     3061 aatcaaatgc cggataaatc actgcaagcg tgtcagccat agataagggt aaatttttca
     3121 tcatctctcc ttacggaaga agttgtcatt atctactgga ttacttaaaa aaacaactta
     3181 ttcattttat gctatttttg ccccatcacc gcaatatgta ccgtaatttc ctcacggtcg
     3241 tggtagaggt gtttagcttg cattttgaac caaaacccgt gtttcgttag ctcttcttcc
     3301 aagaaattga ggcatagttg cacttcttga taacgttttt tcatcggcag ttttaggttg
     3361 aaaatcgttt ctcgacacca accgttagtc agccattttg caatgagttt cgagatacga
     3421 atcggctgct ctaccatatc acaaaccagc caatcgattt gtttacgctt aggcggttgg
     3481 aatttaaacc catcttcagg gcaatgctca attcttccgg tttcgtgtaa acttgctgcc
     3541 attttgccgt gatcgactgc atagacaaaa acaccacgtt tgactaattg ataagtccaa
     3601 ccacccggac aagccccaag atcgacccct accatcgtat cattaaagcg ttttttctcc
     3661 tctgcttgcg gaataaaagt taaaatcgcc tcttctaatt taagcgttga gcgactaggt
     3721 gcctcagccg ggaattttaa acggggaatc cccataaaaa acggcgattt attatggttg
     3781 tacgcatagc ccacatagca agctcccgga cgagtaaata aaatatgcaa gctcacacta
     3841 ccctgcatat tttccgattt ccccaaccaa ccttgttttt tcaatgccga ccgtaatggc
     3901 acagtaaatt tacgacagaa agttaataat tctttcgctt cgttggtatc cggtgtttcg
     3961 acaaatacat cagaactttg gcgaggatta agttcggcat attgggctaa aatcggcgaa
     4021 attcgatcat tttcagacag attttgcaac aaatccccaa caaccaacat ttggcgaaca
     4081 aaaataagct gttcaaactt taacgcttgg gctaatcgat ctgcttcccc tgcttgatag
     4141 cactcaaaaa tcacatagcc actattttct ttaaggttgg caaagccaaa aatgccctgt
     4201 tgtgcagctt tatccgtgat ttcgcctgct acctcttttt caaaccctgc tcggcaatat
     4261 aaggctaatt tattgaacat taggcttttc tcacactgat ataaattaac gcaatccaac
     4321 caagcataaa gcagagacca ccaataggcg taagccaatg aatagtcggc acatttaacg
     4381 ccagtaagta caggttgcca ctaaaaagca gaatgcctaa cgcccaattg cttccgatga
     4441 tgttaaaggc tttggctcta caaacgggtg gattttgccc aaaatttgca atttggaata
     4501 aacctaaagc taaaatggca agggtgtgaa acatttgata tttaatcccc gtctcaatcc
     4561 agcctaaggc tttaggcgat aattgagccg ataatgcgtg gctggctatc gccccaaatg
     4621 ccacagtaaa taagccgctg aatgcggcaa ttgctacaaa tttgtttttc ataagttgaa
     4681 gataggtatg atgttagatg gcttactata tcatattatc gaaaccaaca agcggtgaaa
     4741 ttttgcaaaa aaattacaaa aaatcaccgt ttgtatgctt aatggttcaa gataatatcc
     4801 acatcttccg atttatcgtg aaccccaaat ctatctacta aggttttgct ggcctcattt
     4861 aatccgataa tttctacctc aacatcactt tttcgatatt tcaacacaac tttatctaac
     4921 gctgaaactg ccgttacgtc ccaaaagtga gcgtgggtta aatcaatgtg gacttttttc
     4981 agttctgaac gggtaaaatc aaattgtgcg ataaaatcat cagatgaggc aaaaaatact
     5041 tgcccgataa catcataacg caattcctct ttctctatag cacttgtttt aaccgccata
     5101 taacgtccaa ttttattcgc aaagaataat gccgagagca acacgccgga aaatacacca
     5161 agggctaagt tatgtgtata aaccacaatt gcaacagtga ctaacatcac tacattaaag
     5221 ccgattgggt gcgtttttag ctcttttaag gaatgccaat taaaggtgcc gatagagacc
     5281 ataatcatca ctgcaactaa tgcagccatc ggaatcactc tcaaaatatc gctcaaaaag
     5341 accacaaaaa tcagtaataa tattggcaat gccctgccct ttacattcac ggtttttatc
     5401 gctgtctgtc tctgtcattt catcaacaat cgttgccgtc attaaagatt ccaataaccc
     5461 tactgcggcc aagctgaaag aataaggcaa aataatccat aaggtgtcta aattaagcgg
     5521 aatatcgggt aataaaaaca ccggtaaggt atcgggtaat tgccccatat ccccaacggt
     5581 acgaacatca atgccttgcc ataatgtgaa aagtgttaaa gcaataatgg tcactaatgg
     5641 tgagggaatg atttttccaa tcaccggtac ataaggaaaa agataaatga tagcaagccc
     5701 tgctaccacc agtgcataag tatgccaagt tgtattttgt aactcaggta gttgtgccat
     5761 aaaaattaaa atggcaagtg cattaacaaa cccaatcact acggctcgtg ccacaaaccg
     5821 cataagcgag ccgagtttta accaaccggc aataatttgc aacacgcctg ttaataaggt
     5881 ggccgctagc atatattgta aaccgtgatc cctcactaag cctgctaata ccagagccat
     5941 tgcaccggtt gctgcagaaa tcatcgccgg tctgccgccc acaaaactga taattactgc
     6001 aatacaaaat gaggcatata aaccaatttg tggatcaacc cctgcaataa ttgaaaatgc
     6061 tatggcttct ggaattaacg ccaaagccac cactaaacca gagagaacat cacctcgtac
     6121 atttgaaaac cattgttgtt taattaccgt aggcgaaaat tgctgaaata aagttgtcat
     6181 tattaccctt aaataaaaag aaaagccgag caaagtcggc taaaaatgag gcgagagagt
     6241 agcaaattag tgaaaagtcg tcaaataaac aagcggtctt ctttcgctct ttttttgcaa
     6301 aaaatagtag aaaaatgacc gcttggatta attgctattc actaaaaaaa ggggctttaa
     6361 acaccgtcac ttcaggtgca tctcccacat atttttccac ttgaaccgtg gtagtgttcg
     6421 gtgcattgcc ctgcccgagt tttgatgaac cctcattacg ggttagcaca ttcgggttgc
     6481 cgtttttgca tagtggagta tctgttttgc ctaaatcttg cgggtcatac caaccacctt
     6541 cgtataacgc tactgtgcct tgaatgatgc catcagtcac cactgctcct gccaacacct
     6601 gccctcgctt gttaaaaatt cgcacaatat caccgcttgt aatgcctctg tcagcggcat
     6661 cttttgtgtg aattagcatt ggttctctgc cgtttacctc gtaatattga cgtaatgagg
     6721 agtaagcaag ctggctgtgt aaacggtatt taatatgcgg tgtaactaat gccaacggct
     6781 cggcttcgtt ggttttgcct gaatattcgt gatgttcaaa ccaagtcggg tgtcctttac
     6841 aatcttcata attcatcttg gcaatggttt cggagaaaat ctcaattttg cctgatggcg
     6901 ttgccaattt gtgtttaatc ggatcagtac gataatcttg atagcgaacg aaagtattat
     6961 tttgatgttc tgcctcaaag ctcaacactt tattttccgc ccaaaatgcc tcgaaatcca
     7021 gcaatggcgt attagctttt tcccccgcct caaaggcaac ttggtaaaat tccttaagcc
     7081 attccatttc gcttttgcct tcggtaaatg cttgttcttt accggctcgt ttcgctaatt
     7141 cggcaaaaat atcgtaatca ttcttcgcct caaattgcgg ttcaaccacc tgtttcatcg
     7201 ggataatatg ttttgctaca taatcaccgg caacggtgag atcgtttcgc tcgtagctgg
     7261 tggttgcggg caatacaata tctgccattc gagcggtcgg tgtccagtaa cattcattga
     7321 cgatcatcgt ttccggctgt tgccacgctt tcactaaata gttggtatcc gggtggtgag
     7381 caaacggatt accccccgcc caatagacta atttgatgtt cgggtaatgt aatgtttgcc
     7441 cgttgtaatc ataaggctga ttcggtttga gcaacgcatc ggcaattcga gacaccggga
     7501 aaaacggcat atcggggtta ggtttacctg cattgattgc ccctaaacga acaccggaat
     7561 taacatttag cccattggta gaataacgat aatttaagcc gaaaccgccg cccggcaagc
     7621 caatttgccc gagcatagcg gcaagagtca ctaacgccca gtggctttgc tccccgtgtt
     7681 gcaggcgttg caagccccag ccggacatca acattgtgcg gtgagtaaca aaatcatttg
     7741 ctaaggtttt aatcacctcc tccggaatgt cacaaatttg gctcgcccac tctgctgttt
     7801 taggtaaatt atctgctttt cctaacacat aatcagcaaa ttgctcgaaa ccaacagtat
     7861 atttagttaa gaagtcgtga tcgtgcttgt tttcagtaat aagggtgtgg gcgatcccta
     7921 aaatcagtgc aacatccgtg ccggtattaa taggcaacca ctctgcctct aaaaattgac
     7981 aggtctcgct tttcaccggg tcgatacaaa taatccgttt accgcttgct tttaatttgg
     8041 cgagataatc aatcccctcc ggttcggcag aggtccacgc aatgcgaagt gtattcagtg
     8101 gattagacga ccaaattacc aataattctg tgctttctac aattgtgtgc caactggttt
     8161 gttgagcata cacctcaata ttgcctaata cataaggcat aatcacttgc accgcaccgg
     8221 tggaataatc gcctttagta ttggtaaagc cgcctgtgac gtttaaataa cgttgcaaaa
     8281 gcgtgcgaga ggcgtgcagt gaaccactgc tataccagcc gtaagaaccg ccgaaaatgg
     8341 attcgctgcc atattgctct cttacccgtt ggatttcgcc ggaaaccagc tcaaaggctt
     8401 tgtcccaaga gattctcacc cacttatctc gccctcgcaa agtggtgtct ttattccctt
     8461 ccaagtagcc tttccgcacc atcggatatt tcacccgaac cgggctatag agctgatccg
     8521 ccaccacatc ttgcaattcg ttttggtgat ttttcgctaa actgccctct gatttcacca
     8581 cttttccgtt ttccaccgtt acatttaatg caccccagtg ggcggcggtg gtaatggtat
     8641 ttttcggttt atccaaaaag acaaacttcc ctttttctaa tgctgtgttc atttaaatta
     8701 atgccttttc tgttacaaaa ttgagaggaa tttactggat taagccaaaa gttgcaacca
     8761 caagcggttg tttttagcca aaaatttgca aaacaaaacc cacctaaaaa ggtgggcttc
     8821 aaggaagtta aacggcaact tgaacgatta cattgtccgc ttttttcaca taactattga
     8881 tttggtcaaa gttcatatag cggtaaatat cgtccttatc tttctgcaag ttattcatat
     8941 agtctaggta ctcttgcggt gtcggcaatt tacccaataa cgcagaaact gcggctaatt
     9001 ctgctgaggc taaatacaca tttgcccctt gccctaaacg gttcgggaag tttcgggtag
     9061 aggtagaaac cacattagca ttatttgcca ctcgggcttg gttacccata cagagagaac
     9121 agcccggcat ctctaccctt gctccgcttt tgccgtaaat gctgtaatag ccctcttcgg
     9181 tcagcaagcc tgcgtccatt ttagtcggcg gagcaatcca taatttagtc ggcaattcgc
     9241 cctgtgcttg gcttaacagt ttacctgccg cacggaaatg cccgatatta gtcatacaag
     9301 aaccaataaa gacttcatca attttatcgc cctgtacttg agaaagcagg cgagcatcgt
     9361 cagggtcatt cggtacgcac acaatcggct ctttgatttc atctaagttg atctcaatca
     9421 ctgcggcata ctccgcattt tcgtctgcct ctaaaagctc cggattatcc aaccacgctt
     9481 gcatattggc gatacggcgt tctagcgtgc gtttatcgtc atagccttcc gcaatcatcc
     9541 attttaaaag cgtaatatta gaattcagat actcgataat cggctctttg ttgagcttga
     9601 tggtacaggc tgcagcagag cgttcagcag aagcatcaga aagctcgaag gcttgttcga
     9661 ttttcagatc ttccaaccct tcaatttcta aaattcgacc ggagaaaata tttttcttac
     9721 cttttttctc caccgtcagc aagccttgct gaatggcata atacggaata gcgtgaacca
     9781 gatcccgtaa ggtaatgccc ggttgcattg tgccggaaaa acgcaccaag accgattccg
     9841 gcatatctaa cggcatcacc cctgttgccg cagcaaaggc aactaagccc gaaccggcag
     9901 ggaatgaaat cccgattggg aaacgggtgt gggaatcgcc gcctgtaccg acagtatccg
     9961 gcaataacat acggtttagc caagagtgaa tcacgccatc acccgggcga agtgacacac
    10021 cgccacggtt cataataaaa tcaggcaaag tgtggtgggt aattacgtcc accggtttcg
    10081 ggtaagcggc agtatggcag aacgattgca tcacaagatc tgccgagaag cctaaacagg
    10141 ctaaatcttt cagctcatct cttgtcattg gtcctgtggt atcttgtgaa ccgacagacg
    10201 tcattcgagg ctcgcaatat tgccccggtc gaacgccttc tacgccacaa gctcgcccta
    10261 ccattttttg agcaagggtg tagcctttgt tactctctga aaccgactgc ggtttaataa
    10321 atacatcact ttcaggcaag ccaagtgcta ctctggcttt atgggtcaaa ccacgtccga
    10381 taatcagcgg aattcgcccg cctgcacgca cttcatcaag caatacttcg gtttttagct
    10441 caaattcggc taagacttca tcggagttgt gtttgcaaat tttgcctgca taagggtaaa
    10501 tgtcgattac atcgcccata ttcaggttgg ttacatccac ttcgatcggt aaagaaccgg
    10561 catcttctaa tgtgttaaag aaaatcgggg caattttacc gcctaacacc accccaccgg
    10621 cacgtttgtt tggaatgtaa ggaatatctt cccccataaa ccaaagcacg gagttggttg
    10681 cggatttacg agaagagccg gtgccgacta catcaccgac ataggctaac gggaaacctt
    10741 tttcttttaa tgcttccagt tgcttaatcg ggccggtatg accgttttca tcggggaaaa
    10801 taccgtcacg ttcatttttc agcattgcta atgcgtgaag tggaatatca gggcgagacc
    10861 aagcgtcttg agccggggaa agatcatcgg tattggtttc gcctgtgact ttaaataccg
    10921 ttaccgttaa tttttcggca agtttcgggc gagaggtaaa ccaatcggca ttcgcccaag
    10981 attccaatac ttgttgtgca aacggattac cctgatctgc ccgttctttt acatcgtgga
    11041 aattatcaaa catcaataat gttttagata aggcttctac cgcaatcggt gcaagctcag
    11101 ggttatccaa cgcagacaat aaggtttcaa tgttataacc accttgcatt gtgccgagta
    11161 atttcactgc gtgagcaggc gaaatgagcg gcgatgtggc aacccctttg gtaatatcgg
    11221 ctaaaaaggc ggctttaaca taagcggctt catcaacacc tgccggaata cgattggtaa
    11281 aaagatccaa taaaaagacc gcttgttcag ccggtgggtt tttcaataat tcaaccagtt
    11341 gagccgtttg ttccgcagaa agtggctgag gcacaacacc cagtgccgca cgttcatcaa
    11401 cctgttgttg ataattttct ataaaatttt ccatcatcat tccctctcca actttacata
    11461 ttggcaataa tgtcttgtgc aaattctgag gttgagcgta gagttacccc ctccagcatt
    11521 tccgcaaaat caaaggtgac agttttattg gcaatggttt tagacaccgc ttttaccact
    11581 aaatcagccg cttcaaccca acctaaatgg cgtaacatca tttccccact taaaattaat
    11641 gacccaggat tgcctttatt ttgccctgca attttagggg ctgtgccgtg tgtggcttca
    11701 aaaatagccg cttcaaaccc aatattcgct cccggtgaaa taccgattcc gcctacttgt
    11761 gcggctaaag catcagaaat atagtcgccg tttaggttca gtgtagcaat cacatcatat
    11821 tcggttgggt gtaacaagat ttcttgtaaa aaggcatcgg caatgctgtc ttttataatg
    11881 atttcctcac cggttttcgg attagtcaag ctcatccaag ggcctttatc aattaatttt
    11941 gccccaaatt cttccgcaac ttggtagccc cactctttaa atgctccttc ggtaaatttc
    12001 ataatgttac ctttatgcac aagcgtgagc gatttacggt tgttatcaat tacatattgc
    12061 aatgcagcac gcaccaagcg ttgtgtgcct tgcttagaaa ccggcttaat tccgataccg
    12121 caatcttcgg taaagcggat ttttttcacg cccatctctt gttgtaaaaa ttcgatcact
    12181 ttattggctt caggcgaacc ggcaacccat tctacaccgg cataaatatc ttccgagttt
    12241 tcacggaaaa tcaccatatc caccagctca gggtgtttaa ccgggcttgg cgtaccgtca
    12301 taataacgaa ttgggcgtaa gcagttgtat aaatctaatc cttgacgcat cgccacattt
    12361 aaagaacgaa taccgccacc caccggtgtc attaaaggac ctttaatcgc cacgtgatat
    12421 tggcggataa gctctaaggt ttcatccggc aaccaagtgt tctcaccata taatttattc
    12481 gccttttcgc ctgcgtaaat ttccatccaa gcaatttttc gcttgccttg ataggctttt
    12541 tctaccgccg catctagcac tgctttcatt gcaggagtaa catcaacacc gatgccgtca
    12601 ccttcaataa atggaataat cggattatcc ggaacttgta aagaaccgtc ttggttcagt
    12661 tgaatagttt cgccttgtgg aatttggact tgtgtttgca ttgattttac ccccgtaatg
    12721 acgaaaaagt agtgacgaaa aatgaatgtt gacaatcagc caacaatgga ttaactataa
    12781 acagtaaatt tctaaatgta aataaagcaa aatgggctga gagagaattt gtgatctaac
    12841 gcacatttat gtaaataaaa tgtaaaaaat cgttgtgatt cttttggaaa tc
//
LOCUS       DANLUQ010000061        12769 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.061, whole genome shotgun sequence.
ACCESSION   DANLUQ010000061 DANLUQ010000000
VERSION     DANLUQ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12769
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.061"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            73..1527
                     /locus_tag="PXB27_001341"
     CDS             73..1527
                     /locus_tag="PXB27_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HDL5346126.1"
                     /translation="MKKIVSTLLILTSNLAFAETTPAQLQEKMDNQRMQKNLHTLEKP
                     EPQAKPQAVNAQAHRQSISISSQQLLQQPELLNSAMLTALVTNHTENVQFLLPIYQQL
                     PASKQNSEVVQWAEAVNAKAKQNYSEAIKGYRQLLAKYPENQPIRMQLAVALFENREN
                     EAAEAQFYKLQADKLPQEIANAVNSYLEAIGKQDQWSFQGGLTYLNDPNINNAPNAGT
                     TYGNWTAPKKESAQGVGFHFEADKKWSWGNGFFHEFRLNGNGKYYWNNKKYNEYSLRE
                     SLGLGYQNAKQKITLLPFFEQMWYAGGSSQTESTKRYSNAGGVNARWQYWITPQWQSA
                     ISYEYAEQRYTRRQHLDGNYHFIAPSLYFYPNSRQYWFVGANFNRTSPRDLDDSFIRR
                     GVSIGWGQEWKNGLSTQLSASYARKQYRAPMPIFQITQRNSEYGVQASIWHRALHFKG
                     ITPRLTWQYNKVKSNHSFYSYDKNRVYIEFSHTF"
     gene            complement(1575..4172)
                     /gene="mutS"
                     /locus_tag="PXB27_001342"
     CDS             complement(1575..4172)
                     /gene="mutS"
                     /locus_tag="PXB27_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDL5346127.1"
                     /translation="MTQDFSTHTPMMQQYLQLKAQNPDILLFYRMGDFYELFYDDAKR
                     AAALLDISLTKRGQSAGEPIPMAGVPYHAVEGYLAKLVALGESVAICEQIGDPATSKG
                     PVERKVVRIVTPGTVSDEALLPERQDNLVAAVYEEKGTFAIATLDMTSGRFLISELAS
                     KEALSAELQRVQPAEILYAEDFSAASILTNYKGLRRRPVWEFELVTAIQLLNRQFGTQ
                     TLAGFGVEKAVVALCAAGCVLHYAQETQRTALPHINAIHLAQHSDTILLDAATRRNLE
                     LTQNLAGGTENTLAAVLDKCVTPMGSRLLKRWIHQPIRDCGKLQARQKAITALIQYER
                     MSDLQPLLQQVGDMERILARVALRTARPRDLTRLRTALAQLPEIAKNSQNLTASIDQL
                     LAQLADFSPLHDLLERAIIENPPQLIRDGGVIAEGYNAELDEWRQLSDGATQYLENLE
                     IRERESTGIDSLKIGFNAVHGYYIQISQGQAHKAPIHYVRRQTLKNAERYIIPELKTY
                     EDKVLKAKGASLALEKQLYDELFDVLMPRLGELQLAAMMLSELDVLTNLAERADSLGY
                     VCPSFSPMRMVNIKGGRHPVVEQVLTEPFIANPVFLNSQRHLLIVTGPNMGGKSTYMR
                     QIALITLMAYIGSFVPAESAEIGPIDRIFTRIGASDDLASGRSTFMVEMTEMANILHQ
                     ATENSLVLIDEIGRGTSTYDGLSLAWACAEWLAKKTQSLTLFATHYFELTGLPNQLKG
                     VANVHLDAREHQDSIVFMHSVQEGAASKSYGLAVAALAGVPKQVIQLAKQRLAHLEEI
                     SHQTKQANEHPQADLLFSDGFDLQKNEAIQPLVVENEVEKALKALQPDELTPRQALDE
                     LYRLKKLLG"
     gene            4322..4885
                     /locus_tag="PXB27_001343"
     CDS             4322..4885
                     /locus_tag="PXB27_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5358 domain-containing protein"
                     /protein_id="HDL5346128.1"
                     /translation="MLRKIVLLSTGLLLAACSSNEPQQPEAFPGEFANADYVLSEENA
                     QKWVIASEQAKQCIYPNLTRIQQEHFSKEDSYIFSQYVFFYPLEEIIGEQYVKIIQND
                     EKSMGYAQYLFKKLKNNEAVEALPKKQCATLRTNAKNDLAVVKGQYQSGMIEETNSQE
                     GKSGDKVATNDNKFFFDIIKWGSMLLL"
     gene            5059..5688
                     /locus_tag="PXB27_001344"
     CDS             5059..5688
                     /locus_tag="PXB27_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDL5346129.1"
                     /translation="MAVSATSFAQTTQPTAAEQMNAVKASVAEKATVAKEATKEKAAE
                     LKAGSAEKMAIAKAKTESFKDSAKNKMDAVKATTAEKTDVIKAKAASASDTMKVKAQE
                     AKAATAEKAATAHETLNSAKTATAEKAASMKAAATEKMSAVKKSQKVNINTANEEQLQ
                     SLTGIGEAKAKAIVEYRKKHGKIKNAAELANIPGIGDATIEKVAPYLTF"
     gene            5836..6465
                     /locus_tag="PXB27_001345"
     CDS             5836..6465
                     /locus_tag="PXB27_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007526112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL5346130.1"
                     /translation="MALDFNSVGLDSVEAKGGYGIGLQIGQQLLGSGMDVNAETVARG
                     IYDVLNNNEPAIDINEVTAALQELGQRAEAAQAEAFKAIDAENKAFLEENKKANGVIV
                     TDSGLQYEILTEGTGEKPTATSTVRVHYTGSLIDGTVFDSSVKRGQPAEFPVNGVIRG
                     WTEALQLMPVGSKWRLTIPQELAYGERGAEASIPPFATLVFEVELLDIL"
     gene            6531..7046
                     /locus_tag="PXB27_001346"
     CDS             6531..7046
                     /locus_tag="PXB27_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HDL5346131.1"
                     /translation="MQKKSLISRLLKNIFLYGSLFIIISLVVDWYRSPSAPAQFEQTV
                     HYDIQHQPKVIAQLSHEKPMLLYFWGSWCHYCQVVSPNIQQLANDGTEVLGVALKSGD
                     ENDVQAYLNENGYTFATLNDPSGDFSRGWNIQATPTILIIKDGKIAHHTTGYTSYLSL
                     KFRLWLADVLY"
     gene            complement(7094..8024)
                     /locus_tag="PXB27_001347"
                     /pseudo
     CDS             complement(7094..8024)
                     /locus_tag="PXB27_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433453.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(8083..8733)
                     /locus_tag="PXB27_001348"
     CDS             complement(8083..8733)
                     /locus_tag="PXB27_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595-like element ISMha4 family transposase"
                     /protein_id="HDL5346132.1"
                     /translation="MRKSRLSQYKQNKLIEQFVAGVTARTASELVNVNKNTAAYYFHR
                     LRLLIYQHSPHLEMFEGEIEVDESYLGGHRKGKRGRGAAGKTAVFGLLKRDGKVYTVV
                     VPNTQFATLLPIIREKVKPDSIVYTDFYRSYDVLDVSEFNHFRINHSTHFAENQNHIN
                     GIENFGSQAKRHLRKFNGIPKVHFELYLKECEWRFNHSDLKSQISFLKQLVKGSLG"
     gene            9062..10420
                     /gene="mnmE"
                     /locus_tag="PXB27_001349"
     CDS             9062..10420
                     /gene="mnmE"
                     /locus_tag="PXB27_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDL5346133.1"
                     /translation="MKDTIVAQATPIGRGGVGILRVSGPLAETVAQAVLGKTLPPRIA
                     NYLPFKDEDGTVLDQGIALFFKAPNSFTGEDVLELQGHGGQVILDLLLKRILQVKGLR
                     IARAGEFSEQAFLNDKLDLAQAEAIADLIDATSEQAARSALKSLQGEFSNKINALVDS
                     VIYLRTYVEAAIDFPDEEIDFLADGKIEAKLNEIIAQLDGVRREAKQGSILREGMKVV
                     IAGRPNAGKSSLLNALAGREAAIVTNIAGTTRDVLREHIHIDGMPLHIIDTAGLRDAS
                     DEVERIGIQRAWEEIAQADHVLLMIDSTEQQADQFRQEWAEFLAKLPANMPVTVIRNK
                     VDLSGEQEGLIQVDDFTMIRLSAQTKVGVDLLREHLKKSMGYQSSTEGGFLARRRHLQ
                     ALETAAEHLERGHIQLTQFLAGELLAEELRMVQNALSEITGQFTSDDLLGNIFSSFCI
                     GK"
     gene            10421..11686
                     /gene="tilS"
                     /locus_tag="PXB27_001350"
     CDS             10421..11686
                     /gene="tilS"
                     /locus_tag="PXB27_001350"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDL5346134.1"
                     /translation="MALFNRFQTQCATHLPNQTAFLIGLSGGVDSVVLLHLFARTSFH
                     VRAIYVHHGLSPNADDWAAFCEQYCKRLNIPFILQKVRVDSSNGVENGAREARYQAIQ
                     QQLKPNEVFATAHHLDDQAETFLLALKRGSGVKGLSAMQAVTFLQNFTVFRPLLTFTK
                     SDLIGYAVQHQLGWIEDESNADNRYDRNFLRNSILPLLNQRWQHFSQMVARSAQHCAE
                     QQALIEELLSNELKSRTGEKQQLNINGFGQFSLAKQQQLSRLWLEQNGVRMPSQAQLQ
                     AVISELIFAKADKNPQVKVGENIIRRYQQAIYVTEEIADIPPFEVVLPTETKVELPYQ
                     LGTIIRQQQEIICKKNGKNHRLQLPQELAQSTISIKIGQLGKVKCYGKAQREEMKKIW
                     QNHGIPVWERNRTPLIFWQDELVGLLPIL"
     gene            complement(11676..12569)
                     /gene="xerD"
                     /locus_tag="PXB27_001351"
     CDS             complement(11676..12569)
                     /gene="xerD"
                     /locus_tag="PXB27_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDL5346135.1"
                     /translation="MAELDPIIEQFLDTLWQEHGLSENTVASYRSDLERFSDYFSEPK
                     AFLSLDHIDLQGFLGERLEQGYKATSSARMLSCLRKFFRFLCLEHYRQDDPTLTLSSP
                     KRGASLPKSLSEDQVMDLLDAPNTLDPIELRDKAMLELLYATGLRVTELVSLSMDNIS
                     LKQGVVRIIGKGDKERLVPLGEEANYWIQEFFQYGRPILLNNQQSDVVFPSRRGQQMT
                     RQTFWHRIKHYAVLAGIDADKLSPHVLRHAFATHLVNHGADLRVVQMLLGHSDLSTTQ
                     IYTQVAKARLKSLHQQFHPRG"
     gene            complement(12686..12762)
                     /locus_tag="PXB27_001352"
     tRNA            complement(12686..12762)
                     /locus_tag="PXB27_001352"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12726..12728),aa:Asp,seq:gtc)
BASE COUNT         3832 a         2974 c         2712 g         3251 t
ORIGIN      
        1 aaaacaacct caaaactctt cactaaaggc tattgaaaaa tagccttttc tattcaaata
       61 aaagatagct ttatgaaaaa aatagtctca acgcttttaa tccttacctc aaacctggca
      121 tttgcggaaa ccacaccggc tcaacttcaa gaaaaaatgg ataaccagcg aatgcagaaa
      181 aacctacata cattagaaaa gcctgaacct caagccaaac cacaagcggt taatgcacag
      241 gctcaccggc aatcgatctc tatttcaagc caacagctgc ttcaacagcc tgagttgctc
      301 aactcggcaa tgctaactgc gttggtcact aaccacactg agaatgttca attcttactg
      361 cctatttatc aacaattacc cgccagtaaa caaaacagcg aagttgtaca atgggcggag
      421 gcagttaatg ccaaagcaaa acaaaattat tcagaggcaa taaaaggcta ccgtcagcta
      481 ctggcaaaat accctgaaaa tcagccaatc cgtatgcaat tagcggttgc attgtttgaa
      541 aatcgggaaa atgaagccgc agaggctcaa ttttataagt tacaagcgga caaattaccg
      601 caagaaattg caaatgcggt caatagctat ttggaggcga ttggcaagca agatcaatgg
      661 agttttcaag gcggcttaac ctatttgaat gacccgaata tcaacaatgc tccaaatgca
      721 ggcacgacct atggcaactg gactgcaccg aaaaaagaat ccgcccaagg ggtcggcttt
      781 cactttgagg cggataaaaa gtggtcttgg ggcaatggtt tcttccacga gtttcgttta
      841 aacggaaacg gcaaatatta ctggaataac aaaaaatata acgaatattc gctcagagaa
      901 agcctcggct tgggttatca aaatgcaaaa caaaaaatca ccttgttgcc gttttttgag
      961 cagatgtggt atgccggtgg ctcaagccaa accgaaagca ccaaacgcta ctccaacgca
     1021 ggcggtgtga atgcccgctg gcaatattgg attactccac agtggcaatc tgcaatcagc
     1081 tacgaatatg cggaacagcg ttacactcgc cgccaacatt tagacggcaa ttatcatttt
     1141 attgccccga gcctctattt ttaccctaac agccggcaat attggtttgt gggggcgaat
     1201 tttaaccgca ccagcccccg tgatttagat gatagcttta ttcgccgagg tgtttctatt
     1261 ggttggggac aagagtggaa aaacgggctt tcaacccaat taagtgccag ctacgcccgc
     1321 aaacaatacc gagccccaat gccgattttc caaattacac aacgtaacag tgaatatggt
     1381 gtgcaagcca gcatctggca cagagccttg cattttaagg gtattacccc tcgcttaacg
     1441 tggcaatata acaaggtaaa aagcaatcat agcttctata gttatgacaa aaaccgtgtc
     1501 tatattgagt tttcacatac gttctaaaca aaaacgcccc ttaaacgggg cgatttctta
     1561 gttatttttt caatttaccc caacaatttt ttcaagcgat acaactcgtc caaagcctgt
     1621 cttggggtaa gttcatcagg ctgaagggct ttcagggctt tttcaacttc attttctact
     1681 acaagcggtt gaattgcttc atttttttgc aaatcaaaac catcggaaaa caacaaatcc
     1741 gcctgtggat gttcgtttgc ttgtttggtt tggtgggaaa tttcctccaa atgtgccaaa
     1801 cgctgctttg ccagttgaat cacttgcttt ggcacacccg ccaaggctgc caccgccaag
     1861 ccgtaacttt tgctggcggc cccctcttgc accgagtgca taaacacaat actgtcttga
     1921 tgttcacgcg cgtctaaatg cacatttgcc acccctttga gctgattcgg caagccggta
     1981 agttcaaaat agtgggttgc aaacaaagtg agtgactggg ttttcttcgc cagccattcg
     2041 gcacacgccc acgccagtga taagccgtca taggtagaag tgcctcgccc gatttcatca
     2101 atcagcacaa ggctgttttc ggtggcttga tgcagaatat tcgccatttc ggtcatttcc
     2161 accataaagg ttgaacgccc tgaagctaaa tcatcgcttg ccccaattcg ggtgaaaata
     2221 cggtcaatcg ggccgatttc cgcactttct gccggcacaa aactgccgat atacgccatc
     2281 aatgtaatga gggcaatttg ccgcatatag gtgcttttac cgcccatatt cgggccggtc
     2341 acaatcaata aatgccgttg ggaatttaaa aacaccggat tggcaataaa cggctcggtt
     2401 aaaacctgct ccaccaccgg gtgtctgccg cctttaatat tcaccattcg catcgggcta
     2461 aagctcgggc aaacatagcc taaactatca gctcgctcgg caaggttggt taagacatca
     2521 agttcagaca gcatcatcgc cgctaattgc aattcgccta agcgaggcat taacacatca
     2581 aacagttcat cataaagctg tttttcaagg gcaagcgatg ccccttttgc tttcagcact
     2641 ttgtcttcgt aagttttcag ctcagggata atataccgct ccgcattttt gagcgtttgg
     2701 cggcgaacat agtgaatcgg ggctttgtgt gcttgccctt ggctgatctg aatgtaatag
     2761 ccgtgaaccg cattaaagcc gattttcagc gaatcaatgc cggtgctttc acgttctctg
     2821 atctccaaat tttccagata ttgcgttgcc ccgtccgaaa gttgccgcca ttcgtcaagc
     2881 tcggcattat aaccttctgc aatcacccca ccatcacgaa tgagttgtgg tgggttttcg
     2941 ataatcgccc gctccagcaa atcgtgtaac gggctgaaat ccgccagttg agccaaaagc
     3001 tgatcgatac tagcggtcag attttgtgaa ttttttgcaa tttccggtaa ctgagccaat
     3061 gcagtgcgta gtcgggtgag gtcacgaggg cgagccgtac gcaacgccac tcgggcgaga
     3121 atccgctcca tatcgccgac ttgttgtaat aacggctgta aatccgacat tcgctcatat
     3181 tgtatcaatg ctgttatcgc cttttgtcgg gcttgtaatt taccacaatc tcgaatcggc
     3241 tgatgaatcc aacgcttgag cagacggctc cccattgggg tcacacattt atctaacacc
     3301 gccgccagcg tgttttccgt gccacccgct aagttttggg tcagctctaa attgcgacga
     3361 gtagctgcat cgagcaaaat agtatcgcta tgttgtgcca aatgaattgc gttaatgtgc
     3421 ggtaaagcgg tgcgttgcgt ttcttgggca taatgcaaga cacagccggc agcacaaagt
     3481 gccaccaccg ctttttccac cccaaatccg gcaagtgttt gcgtgccgaa ttggcgattt
     3541 agcagctgaa tcgccgttac cagctcaaat tcccacaccg gacggcggcg taaccctttg
     3601 taattcgtta aaattgaggc agcggaaaaa tcttccgcat acagaatttc tgccggttgt
     3661 acacgttgta actcggcgga tagagcctct ttgctagcaa gttcactgat taaaaaccgc
     3721 ccggaggtca tatctagcgt agcaatcgca aatgtgcctt tttcttcgta aactgccgcc
     3781 accaagttat cttggcgttc gggtaacaat gcctcatcgc taactgttcc cggcgtaaca
     3841 atccgtacca ctttgcgttc taccggccct tttgaggttg ctgggtcgcc aatctgttca
     3901 caaattgcta ccgattcccc caacgccacc aatttcgcca aatacccctc cactgcgtga
     3961 taaggcacac ccgccatcgg aatcggctca ccggcagatt gccctcgctt ggtgagcgaa
     4021 atatccaaca atgctgccgc tcgtttcgca tcatcataaa acagctcata aaaatccccc
     4081 attcggtaaa acaatagtat gtccggattt tgagctttga gttgcaaata ttgttgcatc
     4141 attggcgtat gggtggagaa atcttgtgtc atcagtctat ttattgtgag aaatcattgc
     4201 tgatagttta acagattcag ccaagggata aaaacaagcg gtcatttttc gctgaaaatt
     4261 tgcaatggtc agccgctgtt tttttgttac aatgcaccaa atttttaaaa cagaggtcag
     4321 aatgttaaga aaaatagtgc tactttctac aggcttatta ttggcggctt gtagcagcaa
     4381 tgaaccgcaa caaccggaag cattcccggg cgaatttgcc aatgccgatt atgtgctttc
     4441 ggaagaaaat gctcaaaaat gggtaattgc cagcgaacag gcaaaacagt gtatctaccc
     4501 caatttaacc cgtattcagc aagagcattt ttccaaagaa gattcttata ttttctcaca
     4561 atatgtattt ttctatccgc ttgaagagat tatcggggag cagtatgtaa aaatcattca
     4621 aaatgatgaa aaatcaatgg gttacgctca atatttattc aaaaaactga aaaataacga
     4681 agcggttgaa gcgttaccga aaaaacaatg tgcgacctta cgcaccaatg caaaaaatga
     4741 tttggcagtg gtaaaagggc agtatcaaag cggtatgatc gaagaaacca atagccaaga
     4801 agggaaatca ggcgataaag ttgccaccaa tgataataaa ttcttcttcg atattatcaa
     4861 atggggcagt atgttacttc tctagtgagc ctcaatactc ggttgtttta accacaaccg
     4921 agccatttat acctaccgat tgaactttta tctaaagcct cagtcttaaa atacagtttt
     4981 tttcaattat tttccctctt ttatatttaa ggattttcaa taaaagcatt aaaaacatta
     5041 ttagcattgg gcgtggcaat ggctgtttca gctacttcat ttgcacaaac cactcaacct
     5101 actgcggctg aacaaatgaa cgcagttaaa gcctctgttg ccgaaaaagc aactgtggct
     5161 aaagaagcaa caaaagagaa agcagcggag ttaaaagccg gttctgcgga aaaaatggcg
     5221 atagcaaaag ccaaaaccga atcatttaaa gattccgcta aaaacaaaat ggacgcagta
     5281 aaagccacca cagcagaaaa aacggacgtg attaaagcga aagccgcatc tgcaagcgac
     5341 acaatgaaag tgaaagctca ggaagcaaaa gccgccaccg cagagaaagc tgcaactgct
     5401 catgaaacct taaattcagc caaaaccgca accgctgaaa aagcggcctc aatgaaagca
     5461 gctgcaacag aaaaaatgtc agcagtgaaa aaatcacaaa aagtgaatat caacactgcc
     5521 aacgaagagc aattacaatc gcttaccggc attggtgaag caaaagccaa agcgattgta
     5581 gaataccgca aaaaacacgg taaaatcaaa aatgcagcag aactggcgaa cattcccggc
     5641 attggcgatg caaccattga gaaagttgct ccatatttaa ctttctaatg ttctgcaagc
     5701 ggttattttg ttgcaaaatt ttgcagaaaa ataaccgctt gttcatttca caccttgtat
     5761 tcttaaattt cacccccatt aagtgaatcg tatttctttc aatacagtat tcaataaact
     5821 caaaaggaac agaaaatggc attagatttc aattcagttg gattagattc agtagaagca
     5881 aaaggcggtt acggcatcgg tttacaaatt ggtcagcaac ttttaggcag cggaatggac
     5941 gtaaacgcag aaaccgttgc ccgtggtatt tatgatgtgt taaacaacaa tgagccggca
     6001 atcgacatca atgaagtgac cgctgcatta caagaattag gtcagcgtgc ggaagcggct
     6061 caagcagaag cattcaaagc gattgatgcc gaaaacaaag cgttcttgga agagaataaa
     6121 aaagcaaacg gtgtaatcgt taccgacagc ggtttacaat atgaaatttt aaccgaaggc
     6181 acaggcgaaa aaccgactgc gacttcaacc gtgcgtgttc actacaccgg ttcattaatt
     6241 gacggcaccg tgtttgacag ctcggtaaaa cgcggtcagc cggcagaatt cccggtaaac
     6301 ggagtaattc gtggttggac agaagcattg caattaatgc cggttggctc aaaatggcgt
     6361 ttaaccattc cacaagaatt agcctacggc gaacgtggtg cagaagcatc aattccacca
     6421 tttgcgacct tagtgtttga agtggaattg cttgatattc tctaatcaaa ctcaaataaa
     6481 aaacctgtgg aggcatttca caggtttttg ctatttaaga taactatatt atgcagaaaa
     6541 aatcactcat atctcgctta cttaaaaata ttttcctcta cggcagcctg tttatcatta
     6601 ttagcctggt ggttgattgg tatcgctcgc cctccgctcc ggcacaattt gagcaaaccg
     6661 tgcattatga tattcaacac cagccgaaag taatcgccca attaagccac gagaagccga
     6721 tgctactcta tttttgggga agttggtgcc actattgcca agtggtttcg ccaaatatcc
     6781 aacaattagc gaatgacggc acggaagttt taggtgttgc cctgaaatcc ggcgatgaaa
     6841 atgacgtaca agcctatctc aatgaaaatg gctacacttt cgctactttg aatgacccaa
     6901 gcggtgattt ctctcgaggt tggaacattc aagccacccc aaccatcttg attatcaaag
     6961 acggtaaaat cgcccaccac accaccggct acactagcta tttgagctta aaattcagac
     7021 tatggctggc agacgtgttg tattaaacga ttcctttcat ttgcaaaaaa atccactaaa
     7081 tccgaccgct tgtttacacc aaaaagacaa gcggtactaa attcattgtc atcattgtgg
     7141 tgatcgataa aatcagcctc gatgagccaa aaataaagcc tcggtttttg ccgttgtcga
     7201 atttcaccag taaatttgcc attgctgtga gggcgtacac ttccaccacc gtaaaaatca
     7261 cggtgtaaat ataggcagcg gtcagagttg cgacttttac cgcaataaac caaggggcaa
     7321 taatcgccac cgctaataac ggcgaaatcc aaattaattg ctgtttggtg aggttgattt
     7381 tacgagaaaa agtcgattgc aaactgacgg ttaaaacacc gttgatgaat aacgcaatcg
     7441 gcgaatgctc cggtgcgtgc aggacattgt cgaataaata cggaaaaatc actcagcggc
     7501 acaaatagca ttaagtgagc gaacaaaaac tctttgttga tgatttcctt aatttgtgcg
     7561 aaacgaaatt tcacgccgcc attcacgcct tccaccgaat aaaacggctt gaccataaac
     7621 gcaaacaagc cggcttccaa aataaagcag atccaaatca gcgtttcaat catctcaaac
     7681 ctaataaacg gaataataat cagcggggcg agcattgaag acatactggt gacttttaga
     7741 tatttgcctt gtaaacgggt tttagcagca tattcatcgc cggcaatcgc cagtaaacag
     7801 gctctggcat tagtgccgaa taagcaactc cccaacccaa agcaagtggt tgccaacaat
     7861 aaaatgaggt aattatccgc tgctaaaaac agcatatagg caaccacatc taataaacag
     7921 cccaataaca tcatttttgc caagccgtag cgatcccccc aaatgccggc taaaattgcc
     7981 aatgcctgat tacaaaacaa cagcaatgaa aggaaaaaag cgatttgggc gttatctagc
     8041 cctttctctt ttaaataaaa gaaaaggggc tgtcctagat aactaaccta aactaccctt
     8101 aaccaattgt tttaaaaagg atatttgaga ttttaagtca ctgtgattaa aacgccattc
     8161 gcattctttc aaatacagct caaaatgcac tttgggaatg ccgttaaact tacgcaaatg
     8221 gcgttttgct tggctcccaa aattctcaat tccattaatg tgattttgat tttcagcgaa
     8281 gtgtgtgctg tgattaatgc gaaaatggtt aaattcactc acatcaagca catcataact
     8341 tcgatagaaa tcggtgtaaa caatactatc gggtttcacc ttttcccgaa taatcggcaa
     8401 aagtgtggca aattgcgtgt ttggtactac cacggtataa acctttccgt cccgctttaa
     8461 aagcccgaac acggcggttt tgcctgctgc accacgtcca cgtttacctt tacgatgacc
     8521 gcctaaataa ctctcatcaa cctcaatttc tccctcaaac atttccaaat gagggctatg
     8581 ttgatagata agcaaccgca agcgatggaa gtaatacgcc gccgtgtttt tgtttacatt
     8641 gaccaactca gatgctgttc gggctgttac ccctgcaaca aactgctcaa tgagtttgtt
     8701 ttgtttgtac tgacttagac gactttttct catttgggta tcttaaccta aatggagttt
     8761 ttagtcgtta tctaggacag ccccaaagaa aaacacgctt tgcattgaaa aaaaggcggt
     8821 ggtggaaata aaacggcgta ggagtaatag tttttctaaa gacattcaga atggttgaat
     8881 tggttaaata aagagcggtt attctacctt ttaagtcaac cccaatcaat cggcttttgt
     8941 tttacctttg gcgtgggttt cggtatgata agcgatatgt agtggcgggt cctgtgcccg
     9001 cctaaatacc aatgataact tgcgggcgag cacaggactc gcccctacga taaacaagaa
     9061 aatgaaagac accattgttg ctcaagcaac accgattggc agaggcggtg tggggattct
     9121 gcgagtttcc ggtccgcttg ccgaaaccgt tgctcaagcc gtattaggca aaacattgcc
     9181 accccgtatc gcaaattatt tgccgtttaa agacgaagat ggcactgtgt tggatcaagg
     9241 catcgcctta ttttttaaag ctcccaactc ctttaccggc gaagatgtgc ttgaacttca
     9301 aggacacggt gggcaggtta ttttagattt actgctcaaa cgcattttac aagttaaggg
     9361 gctacgcatt gcccgagcag gtgaattttc cgaacaggca tttttaaacg ataagctcga
     9421 ccttgcccaa gctgaagcga ttgcggattt aatcgacgcc accagcgagc aagccgctcg
     9481 ttcagccctg aaatcactac agggtgaatt ttctaataaa atcaatgcgt tagttgattc
     9541 agtaatttat ttacgcactt atgtggaagc ggcgattgac ttccccgatg aggaaattga
     9601 ctttctggca gacggtaaaa tcgaggcaaa attaaatgag attatcgctc aattagacgg
     9661 tgttcgccgt gaagcgaaac aaggctcgat tctgcgtgaa ggaatgaaag tagtgatcgc
     9721 cggtcgcccg aatgcaggga aatcaagctt gcttaacgcc ttagccggac gagaggcggc
     9781 gattgtaacc aatattgctg gcacaacccg tgatgtgttg cgtgaacata ttcacattga
     9841 cggaatgccg ttgcatatta tcgacaccgc cggtctccgt gatgccagtg atgaggtgga
     9901 acgtatcggc attcagcgtg cttgggaaga aatcgcccaa gccgaccacg ttttattaat
     9961 gattgacagc accgagcaac aagccgatca gttccgacaa gagtgggcgg aatttctagc
    10021 caaactgccg gcaaatatgc ctgtgaccgt aattcgcaac aaagtcgatt tatccggcga
    10081 gcaggaaggc ttaattcagg tggacgattt cacgatgatt cgcctctctg cccaaaccaa
    10141 agtcggcgtt gatttattgc gtgaacactt gaagaaatca atgggctacc aaagttctac
    10201 ggaaggcgga ttcttggctc gccgccgtca tctgcaagca ttggaaaccg ctgccgagca
    10261 tttagagcga gggcatatcc agcttactca attcttagcc ggcgaattgt tagcggaaga
    10321 gttgcgtatg gtgcaaaatg ccttaagtga aattaccggc cagttcacct ctgatgactt
    10381 attaggcaac attttcagct cattttgcat cggtaaataa atggcacttt ttaaccgctt
    10441 tcaaacccag tgtgcaacgc atttgccgaa ccaaaccgcc tttttaatcg gcttaagcgg
    10501 tggggtggat tcggtggtac tactccacct ttttgcccgc acctcatttc acgttagggc
    10561 aatttatgtc caccacgggc taagcccaaa tgcggacgac tgggcagcct tttgcgaaca
    10621 atattgcaag cggttaaata ttccctttat tttacaaaag gtcagagtgg atagctcaaa
    10681 cggcgttgaa aacggggcga gagaagctcg ttaccaagcc attcaacaac agcttaagcc
    10741 caatgaagtg ttcgccaccg cccaccattt agatgaccaa gccgaaactt ttttgctcgc
    10801 cctcaagcga ggcagtggag tcaaaggatt atcggcgatg caagcggtca cttttttgca
    10861 aaattttacc gtttttcgac cgctactcac ttttactaag tcagatttga taggttatgc
    10921 cgttcaacat cagctaggtt ggattgagga cgaaagcaat gccgataacc gttacgaccg
    10981 taatttcctt cgcaatagca tcttgccact actcaatcaa cgttggcagc acttcagcca
    11041 aatggttgcc cgttccgctc agcattgtgc agaacagcaa gccttgatag aagagctatt
    11101 aagcaatgaa ctcaagtcaa gaacagggga aaaacaacaa ctcaacatta acggctttgg
    11161 gcaattttca cttgccaagc aacagcaact tagccgcctt tggctggaac aaaacggagt
    11221 gagaatgcca agccaagccc agctacaagc ggtcatttct gaactgattt ttgcaaaagc
    11281 cgataaaaac ccacaggtta aagtcggcga aaatattatc agacgctatc aacaagctat
    11341 ttacgttacc gaagagatcg ccgacattcc gccgtttgag gtagtacttc ccacagaaac
    11401 caaagtggaa ttaccctatc agctcggtac tattattcgc caacaacaag agatcatttg
    11461 caaaaaaaac gggaaaaatc accgcttgca actgccccaa gagctggctc aatcaactat
    11521 ttctattaaa atcgggcaac taggcaaagt aaaatgctac ggcaaggctc aacgggaaga
    11581 aatgaaaaag atttggcaaa atcacggcat acctgtttgg gaacgcaacc gtacaccgct
    11641 gattttttgg caagacgaat tagttggcct attacctatc ctctagggtg aaattgctga
    11701 tgaagcgatt tcaaacgggc ttttgccact tgagtgtaaa tctgggtggt cgataaatca
    11761 ctatgcccca gcaacatctg taccactcgc aaatcagccc catgattgac caaatgggtc
    11821 gcaaaggcgt ggcgaagcac gtgaggcgag agtttatctg catcaatacc tgcaagcacg
    11881 gcatagtgct taatccgatg ccaaaacgtc tgccttgtca tctgctgacc tcgccggctc
    11941 ggaaatacaa catcagactg ctgattattc agcaaaatag ggcgaccgta ctggaaaaac
    12001 tcctgtatcc aataattcgc ctcctcgccc aatggcacta accgctcttt atccccttta
    12061 ccgataattc tcaccacccc ttgtttgagg ctgatattat ccatcgaaag cgacaccagc
    12121 tccgttaccc gcagaccggt ggcatacagc agctccagca ttgctttatc ccgtaactct
    12181 atcggatcta acgtattcgg ggcatctagc aaatccatta cctgatcttc acttaaggat
    12241 ttgggtaaag aagcaccccg tttaggggaa gaaagggtta aagtcgggtc atcttggcga
    12301 taatgctcaa ggcataaaaa acggaaaaat ttccttaaac aactcaacat tctggcagag
    12361 cttgtcgcct tatagccttg ctccaaacgc tcgccgagaa aaccttgtaa gtcgatatga
    12421 tcgagagaca gaaatgcttt aggttcagaa aaataatcgg aaaaacgctc taagtcagaa
    12481 cgataagagg caacggtatt ttcagacaaa ccgtgttctt gccatagtgt atcgagaaat
    12541 tgttcaataa tagggtcaag ctcagccata caagcgggta tattttcagg aaattttgca
    12601 aattttaaca gaattaaatc tataaaaata caaaagcgat agaaacgaaa aaaccgatta
    12661 gcttatgcta atcggtttaa attattggcg gaacggacgg gactcgaacc cgcgaccccc
    12721 tgcgtgacag gcaggtattc taaccagctg aactaccgct cctaaatta
//
LOCUS       DANLUQ010000062        12737 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.062, whole genome shotgun sequence.
ACCESSION   DANLUQ010000062 DANLUQ010000000
VERSION     DANLUQ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12737
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.062"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..1242)
                     /gene="wecC"
                     /locus_tag="PXB27_001353"
     CDS             complement(<1..1242)
                     /gene="wecC"
                     /locus_tag="PXB27_001353"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HDL5346136.1"
                     /translation="MSNFNTISVVGLGYIGLPTATVFAQHGLNVIGVDVNQHAVDHIN
                     QGKIHIVEPDLDVAVHQCVSSGKLKATLTPEPAEAFLIAVPTPFKGEDYEPDLSYIQA
                     ACKAIAPVLEKDNLVILESTSPVGATEQMAEWLAEYRPELSFPQQAGEDSDIRIAHCP
                     ERVLPGQVMRELIENDRIVGGMTNKCSKQAVDLYKTFVKGDCIVTNARTAEMCKLTEN
                     SFRDVNIAFANELSIICDRLDINVWELIALANRHPRVNILQPGCGVGGHCIAVDPWFI
                     VNKTPDLAKIIRTAREVNDYKPEWVISKVNEAVIEALQKTGKSIDQIKIACLGLAFKP
                     DIDDLRESPALKITEKLAEKYVGQILAVEPNVEELPKKLAKKNISLVTVDEALKLADV
                     VVVLVDHREFRELAAIPSHITV"
     gene            complement(1257..2372)
                     /gene="wecB"
                     /locus_tag="PXB27_001354"
     CDS             complement(1257..2372)
                     /gene="wecB"
                     /locus_tag="PXB27_001354"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HDL5346137.1"
                     /translation="MRYLIVFGTRPEAIKMAPLVEGFKKSGLNFKVCVTAQHRQMLDQ
                     VLELFEITPDYDLDIMSNKQTLSTVTSAILEKIQPVIAEYKPTTIFVHGDTATTLASS
                     LAAYYNQIDIAHIEAGLRTHNIYSPWPEEGNRKLTAALAKYHFAPTQSTKKNLLNENI
                     AEGTIYVTGNTVIDALFLACKKLDENIDLLSSLQEKFSYIKSKRTILITGHRRENFGN
                     GFENICKAVSTLASDFPDVQFIYPVHLNPNVREPVNRLLADKSNVHLIEPCDYLSFVY
                     LMRESYLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVEAGTVKLVGTAQSEIIKQV
                     TELLNDQAAYTKMSEAHNPYGDGTAVKQILSVFQSKQ"
     gene            2605..4695
                     /locus_tag="PXB27_001355"
     CDS             2605..4695
                     /locus_tag="PXB27_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HDL5346138.1"
                     /translation="MLSYLVFFSKIIKIPNLKGFLPHCEIRRRPLVGKTFIAQKRPNQ
                     IIGWGLRPSAVGSRAYAQKHQLPYIALEDGFLRSLGLGVKGYPPFSLVYDDIGIYYDT
                     TCPSRLEKLLLEKPLQDQEQIKLAERALSQILTYGLSKYNHALDFSEIPSAKEKIVLV
                     IDQTFGDMAVKYGQASEVDFNKMLETAISENPEAQIWVKTHPDVLSGKKKGYLTDLPQ
                     YTDRVRIITEDVNPASLIKLCEKVYCVTSQMGFEALLLGKKVVTFGVPWFAGWGVTDD
                     RNEAVQLLVQQNRRKDRSLLQLFYAAYFDYSRYINPNTGEKGSIFDVIDYLYKAKRLN
                     QRLSGNLYCIGMSLWKQAVIKPFFNLPQCRLHFVRSLNSLKKRPLADNAKILVWSNGK
                     ADVLQFAQQNNIPLLRMEDGFIRSVGLGANLVAPLSLVVDDLGIYFNAENTSRLEQIL
                     LSAEHFSETDLSYAETLRQKLVASHIGKYNVGGGDVKFNAQGKKIILVPGQVEDDASI
                     KSGSPQIKRNLDLLKKVRELNPSAYIIYKPHPDVVSGNRVGQIPTAETRRFADQMVDN
                     ANILDCILIADEVHTMTSLAGFEALLRGKKVHCYGLPFYAGWGLTEDRLKSARRGKSL
                     TLSQLIAATLIYYPLYVDPISRQFIDANQAIHILSQQKRNLTNQRIKRSWVSKQYGKL
                     QHLYRAFKYASHTK"
     gene            4718..5950
                     /locus_tag="PXB27_001356"
     CDS             4718..5950
                     /locus_tag="PXB27_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HDL5346139.1"
                     /translation="MEHYLDELIINSQRILLLQGPIGSFFTEFARWLESEQKEVFKLN
                     FNGGDEYFYPNNSPNTFPYYDSLDNFEAYLIQFCQTHQIDNILCFGDNRYYHKVAKNV
                     CLKLKLVFWAFEEGYFRPEYITLEKWGVNAFSPIPKNHTFFLPYAELPVVSEPEKVSK
                     GFRCMAGKAIPYYWNAYKKRKAYAKYQHHRFLNLKYYLNLWTRSGIKRICYWLHDKGF
                     AKRVEKGEFGEFFIVPLQVYDDTQVRIHSEQKSVERFLREVLNSFAKFAPAHLNLIIK
                     HHPMDRGFIDYKMVIYEFLEQYPQLNGRVFYIHDVPLPVFLRYGKGMVTLNSTSGLSA
                     LLHNMPVKTLGRANYDFEGLTDKQPLDTFWANPQPPNSEVFKGYRQFHLHKTHINGNF
                     YHKSILRYPYNWENEAHR"
     gene            6031..7092
                     /locus_tag="PXB27_001357"
     CDS             6031..7092
                     /locus_tag="PXB27_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL5346140.1"
                     /translation="MAIYAIWNNKGGVGKSYLTFQIACEYAKQYPAKKVLVVDLCPQA
                     NSSSMLLGGIEKGEQELAKIHSQVNKNTISGYISERLVSPYRSINSATKYVTQVSTIN
                     EYVPKNVYLVVGDEELEIQSSGILHASQAVHVPDSWRLVHLWVRDLVSDIQNAWDNDD
                     NVVFIDCNPSFTIYTELALAASDRLIIPFSADGSSKRAVKSVLSLVYGVKRHSGDVSS
                     HFYTESQKSKLALPQIYMYVGNRLTINAKASATAFKTVVEEIGDEIYNIWKTNANLFC
                     IHPAGVGTPTNKSSFRKMFQYEVRDANTASVVSGALGIPISRLSSGKRDVLGKNVMVN
                     QSQLDAQVPNIQNLVQCIE"
     gene            7244..8896
                     /locus_tag="PXB27_001358"
     CDS             7244..8896
                     /locus_tag="PXB27_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDL5346141.1"
                     /translation="MTIFQVAQNWLAQDPDAETRAELEQLIQAAESDEKAKAELTARF
                     DGRLQFGTAGLRGRLQAGSMGMNRVLVAQAASGLAGFIKGYDKEPSIVIGYDGRKNSD
                     VFARDTAEIMAAAGIKTYLLPRKLPTPVLAYAIQYFDTTAGVMVTASHNPPEDNGYKV
                     YLGKANGGGQIVSPADKEIAALIDKVAAGDIRDLPRSQDFTVLDDEVVNAYIEKTASL
                     AKRPKAEINYVYTAMHGVGYEVLSKTLEKAGLPQPYLVSEQIQPDGSFPTVNFPNPEE
                     KGALDLAIKLAKEKNAEFIIANDPDADRLAVAVPDAQGNWKPLHGNVIGCFLGWYLAK
                     QFHAQGKQGVLACSLVSSPALAEIAKKYGLSSEETLTGFKYIGKVENLLFGFEEALGY
                     LVDPDKVRDKDGISAAIMFLDLVCSLKQEGKTLADYTTEFVQEFGAYVSGQISIRVCD
                     LAEIGKLMTALRNNPPSEIGGFKVAEFIDHTKTTRQNDILVFVLENGSRLIARPSGTE
                     PKIKFYLDACGTDAENAEQVLAQFDESVRSLLRQEEYGKQDC"
     gene            8949..9883
                     /locus_tag="PXB27_001359"
                     /pseudo
     CDS             8949..9883
                     /locus_tag="PXB27_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250489.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
     gene            complement(9905..11986)
                     /gene="recG"
                     /locus_tag="PXB27_001360"
     CDS             complement(9905..11986)
                     /gene="recG"
                     /locus_tag="PXB27_001360"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDL5346142.1"
                     /translation="MSEQLLDGVPLTALSGVGAAISEKLSRIGINNVQDLLFHLPMRY
                     EDRTRITPIADVRPESFATIEGYVQLTEVQFGKRPILSVTLSDSTSKIMLKFFNFNAG
                     MKNSLATGVRVKAFGEIKRGRFMAEIHHPEYQIIRDNQPLVLAETLTPIYSTTEGLKQ
                     ASLRKLTEQALALLEKVKVAELLPDEYNPHKYSLKEALQLLHRPPPTISAEVLEKGDH
                     PAQKRLIFEELLAHNLAMQQLRLGVKQQYAEALTYQTDIKQRFLDSLPFKPTHAQSRV
                     TADIEQDLAKPHPMMRLVQGDVGSGKTLVAALAALLAIDNGKQVALMAPTEILAEQHA
                     NNFANWLNPFGIEVGWLAGKVKGKARTAQLEAIKNGDVQMIIGTHALFQDNVEFHHLA
                     LVIIDEQHRFGVHQRLTLREKGAKENNYPHQLIMTATPIPRTLAMTVYADLDTSIIDE
                     LPPGRTPITTVVISEDRRAEIVQRVYQVCKQEHRQAYWVCTLIDESEVLEAQAAAAIA
                     EDLQRALPDLRIGLVHGRLKPQEKQAIMAEFKAANLDLLVATTVIEVGVDVPNASLMI
                     IENSERLGLAQLHQLRGRVGRGATASHCVLMYKPPLGKISSKRLQVMRDSQDGFYIAE
                     KDLEIRGTGEILGTKQTGMAEFKVADLMRDRKMIPLVQRYAKQIISENQPLAEALIKR
                     WLDNKAEYTNA"
     gene            complement(11990..12418)
                     /locus_tag="PXB27_001361"
     CDS             complement(11990..12418)
                     /locus_tag="PXB27_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HDL5346143.1"
                     /translation="MYRYLFDTNIVSELYKLRNDKMDVNVRQWLRGVNPSQTNISCIT
                     LSEIKTGILLKARKDKEQSDRLNDWFENNVLKAYQTKAFVVNNDIALLASEYHIPNKM
                     DLNDAYIAATAKYHNLILVTRNTKDFIRCDVRLFNPFETA"
     gene            complement(12418..12705)
                     /locus_tag="PXB27_001362"
     CDS             complement(12418..12705)
                     /locus_tag="PXB27_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HDL5346144.1"
                     /translation="MAITITSREFNQNASKVLKMAEKEPVFITKWGKIVNVVSSYMDY
                     QQKKPSEPSFEELFASSENEDVSDEFWQEFEQTLVEIRKSNKPRPVDFGDE"
BASE COUNT         3704 a         2416 c         2737 g         3880 t
ORIGIN      
        1 aacagtaata tgtgatggaa tcgcagcaag ttctctaaat tctctatgat ccaccaacac
       61 aaccactaca tcagccagtt ttagtgcttc atcaacagta actaaagaga tatttttctt
      121 agccaatttt ttcggtagct cttcaacatt tggctcaact gctaagattt gacctacata
      181 tttttctgcc aatttttcgg taatttttaa ggccgggctt tcacgtaaat catcaatatc
      241 aggtttaaaa gccaaaccta ggcaagcaat tttgatttgg tcgatagatt ttcctgtttt
      301 ttgcaaagct tcaattactg cttcatttac ttttgaaata acccattcag gtttatagtc
      361 atttacttca cgagcagtac gaataatttt tgccaagtca ggagttttat tcacaataaa
      421 ccaaggatct acagcaatgc agtgtccacc cacaccacaa cccggttgta agatattgac
      481 tcgtgggtgt ctgttagcca aagcaattag ttcccataca ttaatatcca ggcgatcaca
      541 aataatagaa agctcatttg caaaagcaat attgacatca cggaaactat tttcggtcaa
      601 tttgcacatt tctgcggtac gagcattggt aactatacaa tcgcctttta caaaagtttt
      661 gtataaatcg accgcttgtt tagagcattt atttgtcata ccacccacaa tacggtcatt
      721 ttcaatcaat tcacgcatca cttgccctgg tagcactcgt tctgggcaat gggcgatacg
      781 aatatcagaa tcttcacctg cttgctgagg gaagcttagc tctggacggt actcggcaag
      841 ccactcagcc atctgttctg ttgcgccaac cggagaggtg gattctaaaa tgactaaatt
      901 atctttttcc agcacaggtg caatagcttt acaagcagct tgaatataac ttaaatcagg
      961 ctcataatcc tcgcctttaa aaggcgttgg tacagcaatt aaaaatgctt ctgctggttc
     1021 aggtgtcagt gtggctttta attttccgct agatacacat tggtgaacag caacatctaa
     1081 atcaggctcg acaatgtgaa ttttcccttg attgatatgg tctacggcgt gctgattaac
     1141 atctacgcca attacattta gaccgtgttg ggcaaataca gtggcggttg gtaagccaat
     1201 atagcctaag cctactactg aaatggtatt aaagttcgac atacatttat cccttattat
     1261 tgttttgatt gaaatactga gagaatttgt ttcacagctg tgccatcgcc ataagggttg
     1321 tgagcctcag acatttttgt ataagcagct tgatcattga gtaactcagt aacttgttta
     1381 ataatttcag attgtgcagt tccaacaagt tttactgttc cggcttctac agcttccggt
     1441 ctttctgttg tgtctctcat tactaatacc ggttttccta atgatggggc ttcttcttga
     1501 atacctccgg agtcagttaa gataaggtaa gactctctca ttaaataaac aaaggagaga
     1561 tagtcgcaag gctcaatgag atgcacattg gatttatccg ctaataggcg attgaccggt
     1621 tctcttacat ttgggttgag gtgtacgggg tagataaatt gaacgtcagg gaaatcagac
     1681 gctaatgttg aaacggcttt acagatgttc tcaaaaccat taccgaaatt ttcgcggcga
     1741 tgtccggtga tcaaaatagt acgcttactt tttatataag aaaatttctc ttgtagcgag
     1801 cttaataaat caatattttc atctaatttt ttgcaagcga ggaataaggc atcaattacc
     1861 gtattacccg ttacataaat ggtgccctca gcaatatttt cgttgagtag atttttcttg
     1921 gtgctttgtg ttggtgcgaa atggtatttt gccaaagcag ctgttaattt gcgattgcct
     1981 tcttccggcc aaggagagta gatattatgc gttcttagac ctgcctcaat atgagcgata
     2041 tcaatttggt tgtaataggc tgctaatgaa gaggcaagag tggttgctgt atcgccatga
     2101 acaaaaatag tagttggttt gtattcggca ataacaggtt gaattttttc taaaatagca
     2161 gaagttacag tagaaagtgt ttgcttattg ctcataatat ccaggtcata atctggagta
     2221 atttcgaata attctaaaac ttggtccagc atttggcggt gctgagcagt aacacagact
     2281 ttaaaattta gaccgctttt cttaaagccc tcaactaagg gagccatttt gattgcctca
     2341 ggacgagtac cgaagacgat gagatagcgc atttattact cctttaaaaa tatagataaa
     2401 actaaaatac actagatgta agtaattttg ttaaattagt tataaagatc gtgttcatta
     2461 taatgttttt atggagttag gtgtataaaa acttttactt tgtagatatt ttatgtaaat
     2521 tcttgtaagt atccggtagg ttagaaatta tcatgttatc tgctatggta caatacataa
     2581 atacgaatat gagattaatt ttttatgctt agctatttag tgttttttag taaaattata
     2641 aaaatcccta atttaaaagg ttttttacct cattgtgaaa ttaggcgaag acctcttgtg
     2701 ggaaaaacct ttattgctca aaaaagaccg aaccaaatca tcggctgggg gcttcgtcct
     2761 tcagcggtgg gatcaagagc ttatgcacaa aaacatcaac ttccctatat tgcattagaa
     2821 gacggctttt tacgctcact agggctgggt gtaaaaggct atccgccatt ttctttagtt
     2881 tatgatgata taggtattta ttacgatacc acctgtcctt ctcgtttaga aaagttattg
     2941 cttgaaaaac ctttacagga tcaagagcaa atcaagcttg cagagagagc attatctcaa
     3001 atcttaactt acggactttc taaatataat cacgcactag attttagtga aatcccctct
     3061 gccaaagaaa aaatcgtgtt agttattgac caaacctttg gtgatatggc ggtgaaatat
     3121 ggtcaagcga gtgaagtgga tttcaataaa atgttggaaa cggcaatcag tgagaatcca
     3181 gaggctcaaa tttgggtaaa aactcacccc gatgtattaa gtggcaagaa gaaaggctat
     3241 ttgaccgatt tacctcaata tactgatagg gtcagaatta ttactgaaga tgtgaatccg
     3301 gcaagtttga ttaaattgtg tgagaaagtc tattgcgtaa cttcgcaaat ggggtttgag
     3361 gcattattat taggtaaaaa agtggttacc tttggtgtac cgtggtttgc cggttggggg
     3421 gtaacagatg atcgtaacga agcggttcaa ctattagttc aacaaaatag acggaaagat
     3481 cgttcattac tgcaactttt ttatgctgcg tattttgatt acagccgtta tattaatccg
     3541 aataccggtg agaaaggctc tatttttgat gtcatcgatt atctatataa agccaaaaga
     3601 ttaaatcagc gcttaagtgg gaatctatac tgtattggta tgtcgctatg gaaacaggcg
     3661 gtaattaagc cgttttttaa tttgccacaa tgtcgattac attttgttcg ttcgctaaat
     3721 tctttaaaaa aacgaccgct tgcagataat gcaaaaattt tagtttggag caatggcaaa
     3781 gcggatgtct tgcaatttgc acagcagaat aatattccat tgttaagaat ggaagatgga
     3841 ttcattcgct ctgtcggact aggcgctaat ttagttgcac cgctttcttt agtagtcgat
     3901 gatttaggca tttattttaa tgcagagaac acttcacgat tagaacaaat tttattgagt
     3961 gcagagcatt tttctgaaac tgatttgtct tatgctgaaa cattaaggca gaaattggtg
     4021 gctagccata ttggaaaata taatgtgggc ggtggcgatg taaaatttaa tgctcaaggt
     4081 aaaaaaataa ttcttgtacc ggggcaggta gaagatgacg cttcgatcaa atcaggttct
     4141 ccacaaatta aaagaaatct ggatttactg aaaaaagtga gggaattaaa ccctagtgcc
     4201 tatattattt ataagcctca ccctgatgtg gtaagtggta atcgagtcgg tcagattcct
     4261 acggcagaaa cacgcaggtt tgcggatcag atggtggata atgccaatat tttagattgt
     4321 attttaattg cagatgaagt gcatacaatg acatctttag ccggtttcga agcattatta
     4381 agaggtaaaa aagtgcattg ctacggatta cctttttatg ctggttgggg attgacggaa
     4441 gatagattga agtcggcacg cagaggtaaa tctttgacat tatctcagtt aattgccgca
     4501 acattgatat attaccctct gtatgttgat cctataagca ggcagtttat tgatgccaat
     4561 caagcgattc atatattgtc acaacagaaa cgaaacctaa ccaatcaaag gattaaacgt
     4621 tcttgggtga gtaaacaata tggaaaatta cagcatcttt atcgtgcgtt taaatatgct
     4681 tcccacacta aataagcgta gagggtagga aattaagatg gaacattatt tagatgagct
     4741 aatcatcaat agccagagaa ttttattgtt acaaggcccg ataggctctt ttttcactga
     4801 gtttgcccgt tggttagaaa gtgagcaaaa agaggtcttc aagttaaatt ttaatggcgg
     4861 agatgaatat ttctatccaa ataatagccc gaatactttt ccttactatg atagcttaga
     4921 taattttgaa gcctatttaa ttcagttttg ccaaactcat caaatagata atatcttatg
     4981 ttttggcgat aatcgttatt atcataaagt ggctaaaaac gtttgcttga agttaaagtt
     5041 ggtcttttgg gcgtttgaag aagggtactt taggccagaa tatattacgt tggaaaaatg
     5101 gggtgtaaat gctttttctc caatccctaa aaatcacact ttctttttgc cttatgccga
     5161 gttaccagta gtcagtgaac cagaaaaagt atctaaaggc tttagatgta tggcgggaaa
     5221 agcaatcccg tactattgga atgcctataa aaaacgtaaa gcatacgcaa aatatcagca
     5281 tcatcgtttt ctgaatctca aatactacct taatttatgg acaaggtcgg gaattaagcg
     5341 aatatgttat tggttacatg ataaaggctt tgccaagcgg gtagaaaaag gagagtttgg
     5401 tgaatttttc attgtgcctc tacaagttta tgatgacact caagtgagga ttcatagtga
     5461 acaaaaaagt gtagagcgtt ttttaaggga agttctgaat tcctttgcca aatttgcacc
     5521 ggcacatctc aatttaatta ttaagcatca cccgatggat agaggattta tagattataa
     5581 aatggtgatt tatgaattct tagagcaata tccccaatta aacggtagag ttttttatat
     5641 tcacgatgtg ccgttacctg tctttttacg ttatgggaaa ggaatggtga ctttaaattc
     5701 aaccagtggg ctttctgcat tgctccataa tatgccggtc aaaaccttag gtagagccaa
     5761 ttatgatttt gagggattaa ccgataaaca gccattagat actttttggg ctaatcctca
     5821 gccacctaat tcggaagtat ttaaaggcta cagacaattt catttacata aaacgcatat
     5881 taacggtaat ttttaccata aatcgatttt gcgttatcct tataattggg aaaatgaagc
     5941 acacaggtga tggaaaatat taaaataagt aatatcttat agctaaatta ttgtgttatt
     6001 attacaccta ttgttaaaag agggggcatt atggctattt atgcaatatg gaataataaa
     6061 ggtggggttg gtaaaagtta tttaactttt caaatcgctt gtgagtatgc aaaacaatac
     6121 ccagcaaaaa aagttctcgt tgttgattta tgtcctcaag caaattcatc atctatgcta
     6181 ttaggcggca ttgaaaaagg tgagcaagag ttagcaaaaa ttcattctca agtaaacaag
     6241 aatactattt caggatatat ttcagaaaga ttagtaagtc cttaccgttc tattaatagt
     6301 gcaacgaaat atgtaactca agttagcact ataaatgaat atgtaccaaa aaatgtttat
     6361 cttgttgttg gtgatgagga attagaaatc caatcatctg gtattttaca tgcttcccaa
     6421 gctgttcatg ttcctgattc ttggcggcta gtgcatcttt gggtgcgtga tttagtttct
     6481 gatatacaga acgcttggga taatgatgat aacgttgtat ttattgattg caatccaagt
     6541 ttcactattt atactgaatt agcgttggct gcttcagata gattgatcat ccctttttcc
     6601 gctgatggtt catcaaaacg agctgttaag tctgtattat ctctagtata tggggttaaa
     6661 aggcactctg gggatgtcag ctctcatttt tatacagaat ctcaaaagag taagttagca
     6721 ttacctcaaa tttatatgta tgtaggtaat agattaacga taaatgccaa agcatcagca
     6781 accgcattta aaacagttgt agaggaaata ggtgatgaaa tctataatat atggaaaact
     6841 aatgctaatt tgttctgtat tcacccagca ggtgttggta ctcctacaaa taaatcaagc
     6901 ttcagaaaaa tgtttcagta tgaggtgaga gatgcaaata cagcatctgt tgtttcaggt
     6961 gctttaggta ttccaattag tcgcttatca tcaggaaaaa gagatgtttt aggtaaaaat
     7021 gtaatggtga atcagtcaca attagatgct caagttccta acattcaaaa tcttgttcag
     7081 tgtatagaat aaagaaaaat agcgtatatt aagtacgcta ttttttattt ctgacaaatc
     7141 tttttatttg atagctcgat cacaaaaaaa gaaatatctc cttttaaata aaaggacttt
     7201 gggctaatct aaacagagtt tttaaattac ataggagtaa aaaatgacta ttttccaagt
     7261 tgcacaaaat tggttagccc aagacccgga tgcagagacc cgtgcggaat tagagcagtt
     7321 aattcaagcg gcagaatcgg acgagaaagc aaaagcagaa ttaacagccc gttttgacgg
     7381 acgtttacag tttggtacgg ctggtttgcg tggtcgctta caagcgggtt caatgggtat
     7441 gaaccgtgtt ttggtggcac aagctgccag cggtttagct gggtttatta aaggttatga
     7501 taaagagccg tcaattgtta ttggttatga cggtcgtaaa aattccgatg tgtttgcccg
     7561 tgacaccgct gaaattatgg cagcggcagg cattaaaacg tacctgcttc ctcgcaaatt
     7621 gccaacacca gtgcttgcct atgcgattca atattttgat accacagccg gtgtgatggt
     7681 aactgccagc cacaacccac cggaagataa cggctataaa gtttatttag gtaaagccaa
     7741 cggtggcgga caaattgttt cgccggcaga taaagaaatt gctgctctga ttgataaagt
     7801 agcagcaggc gatattcgtg atttacctcg tagccaagat ttcacggtgt tagacgatga
     7861 agtggtaaat gcttatattg agaaaaccgc ctcactagcc aaacggccaa aagccgaaat
     7921 aaactacgtt tacactgcaa tgcacggtgt gggctatgaa gtgttaagca aaacattaga
     7981 aaaagccggc ttaccgcaac cgtatttagt tagtgagcag attcagccgg acggttcgtt
     8041 cccaacggtt aatttcccga acccggaaga gaaaggggcg ttagatttag cgattaaatt
     8101 ggcgaaagag aaaaatgccg aatttattat cgccaacgac ccggatgctg accgtttagc
     8161 ggtagccgtg cctgatgctc aaggcaactg gaaaccactg catggcaatg tgatcggctg
     8221 tttcttaggc tggtatttag ccaaacaatt ccacgcacaa ggtaaacaag gcgtgttggc
     8281 ttgctcgtta gtatcctctc cggcgttagc tgaaattgcg aaaaaatatg gtttaagctc
     8341 ggaagaaacc ttaaccggct ttaaatatat cggaaaagtt gaaaacctat tatttggctt
     8401 tgaagaagcc ttaggttatt tggttgaccc agacaaagtg cgtgataaag acggcatttc
     8461 cgctgccatt atgttcttag atttagtgtg cagcctcaaa caagaaggta aaacccttgc
     8521 ggattacacc accgagtttg tacaagagtt cggggcttat gtaagcggtc aaatttcgat
     8581 cagagtttgt gatttagccg aaatcggcaa attaatgacg gcgttacgca acaatcctcc
     8641 aagcgaaatt ggtggcttta aagtggctga gtttattgac cataccaaaa ccacacgcca
     8701 aaatgacatt ttagtgtttg tgttggaaaa tggcagccgt ttgattgctc gcccttcagg
     8761 tactgagccg aaaattaaat tctatttaga tgcttgtggc acagacgctg aaaacgcaga
     8821 gcaagtgctg gctcagtttg acgaaagtgt gcgttcgctg cttcgccaag aagaatacgg
     8881 caaacaagat tgctaatggc attaattgag ggggagctta ggctctccct tttttaaagg
     8941 agacaataat ggctcaaaaa attattttag attgtgatcc ggggcatgac gatgcgattg
     9001 cgatgatgct cgcttggggg aatccgaata ttgatttgtt ggcagtgact acggtggtag
     9061 gtaatcaaac cttagaaaaa gtatcgcgta atgcgttggc ggttgcggaa attgctcata
     9121 tttgcaacgt tccttttgcc aaaggttgtg ccagaccgct ggtgcgtgaa gtggaaaatg
     9181 ccccggatat tcacggtgat tcgggaatgg atgggcctgt actccctgaa tctacccgac
     9241 aatttgagaa aaaacacgcc gctcaattaa ttattgattt gataatgtcg cacccggaga
     9301 aaagcattac cttagtgccg accggtgggc aataaccaat attgctctag ctgcccgcct
     9361 tgagccccgt attattgaaa gagtgaaaga agtggtgctg atgggcggag gttatcacac
     9421 cggcaactgg agtgcggtgg cagaatttaa tattaaaatt gacccggaag ctgctcatat
     9481 tgtgtttaat gccggttgga aggtgacgat ggtcggctta gacttaactc accaagcact
     9541 tgcgaccccc gatgtggttg aacgctttaa ggcattaaac agcaaatcgg ggcagtttgt
     9601 ggtggaattg ctggcgttct tcgccaaaat gtataaacag gctcaaggct ttgatgctcc
     9661 accggttcac gatgcctgtg cggtggcgta tgtgattgac ccaagcctga ttgaagttca
     9721 gcaagtgccg gtcaatattg agctaacagg cacgcataca ctgggtatga cggtggcgga
     9781 tttccgctac ccgccaaaac agtgtaatac ttgggtagca aaaaaattag acaaatcccg
     9841 cttttgggat ttggtaattg aatcggtgaa aaacctgccg taatctaaat aacccgcttg
     9901 ccggttaagc gttggtatat tccgccttgt tgtctaacca acgcttaatt aacgcctctg
     9961 caagcggttg attttcgcta ataatttgct tggcgtaacg ttgcaccagc ggaatcattt
    10021 tgcggtctcg cattaaatcc gccaccttaa attctgccat tccggtctgt tttgtgccga
    10081 gaatttcgcc tgtgcctcga atctccaaat ctttttcggc aatgtagaaa ccgtcttggc
    10141 tatcacgcat cacttgtaac cgcttgctgg aaattttccc gagcggcggt ttgtacatta
    10201 agacgcaatg cgaggcggtt gcccctcgcc caacacgtcc acgcaactgg tggagttgag
    10261 ccagcccgag tcgttcggaa ttttcgataa tcattaggct agcattcggc acgtccactc
    10321 ccacttcaat tacggtggtc gcaaccagca aatctaaatt cgctgcctta aattccgcca
    10381 taatcgcttg tttttcctgt ggtttcagcc gtccgtgtac taacccgatg cgtaaatccg
    10441 gcaaggctcg ctgtaaatct tcggcaatcg ctgccgcggc ttgagcctcc aacacttcgc
    10501 tttcatcaat cagggtgcag acccaatagg cttggcggtg ttcttgctta cagacttggt
    10561 acacccgttg gacgatttct gctcgccgat cttcagaaat caccacagtc gtgattggcg
    10621 ttctacccgg tggcagttcg tcaatgattg aggtgtcgag gtcagcatag accgtcattg
    10681 ccagcgttct cggaatgggg gtcgccgtca taatgagctg atgcggatag ttattttctt
    10741 tcgctccttt ttcccgcaag gtcagccgtt ggtgtacgcc aaaacggtgt tgttcatcaa
    10801 taatcaccaa cgctaaatgg tgaaattcca cattgtcttg gaacagggcg tgagtgccga
    10861 taatcatctg cacatcaccg tttttgattg cctcaagttg tgcggttctc gccttgcctt
    10921 tcactttacc ggcaagccag ccaacctcaa tcccaaaggg attaagccag ttggcaaaat
    10981 tattggcgtg ctgttcggca agaatttcgg tcggtgccat taatgccacc tgcttgccgt
    11041 tatcaatcgc taatagtgct gccaaggcgg ctactaaggt tttacctgaa ccaacatcgc
    11101 cttgtaccag tcgcatcatc gggtgtggtt tggcaagatc ttgctcaata tctgcggtta
    11161 ctcggctttg tgcgtgagta ggcttaaaag gcaaactgtc gagaaaacgc tgttttatat
    11221 cggtttggta agtaagtgcc tctgcatatt gctgttttac ccctaaacgg agttgctgca
    11281 tcgctaaatt gtgtgccagc agttcttcaa aaatcagccg tttttgagca gggtggtcac
    11341 ctttttccaa gacttcggca gaaatcgtcg gcggtgggcg gtgcaataac tgcaacgctt
    11401 cttttaggct gtatttgtgc ggattgtatt catcaggcaa cagctcggcg actttcactt
    11461 tctctaataa agcgagggct tgctcggtta atttccgcag cgaggcttgt tttaacccct
    11521 cagtggtgga gtaaatcggg gtcagggttt cagccagtac aagcggttga ttatcccgaa
    11581 taatttgata ttcagggtgg tgaatttccg ccataaatcg cccacgctta atttccccaa
    11641 aggcttttac ccgaacgccg gtggcaaggc tatttttcat tccagcattg aaattgaaga
    11701 atttaagcat aattttgctg gtgctgtcgg agagtgtaac cgataaaatc gggcgtttac
    11761 caaactggac ttcggtaagt tgtacatagc cttcaatggt ggcaaagctt tccgggcgaa
    11821 catcagcaat cggggtaata cgggtgcgat cttcataccg cattggcaga tgaaacagta
    11881 aatcctgcac attattaatg ccgattcgac ttaacttttc ggaaattgcc gctcctacgc
    11941 cggaaagggc ggttaatggc acgccgtcta ataattgttc actcattatt tatgcggttt
    12001 caaatgggtt aaataaacgc acatcacaac ggataaaatc tttggtgttg cgggtgacta
    12061 aaatcagatt gtggtatttg gcggttgccg caatataagc atcatttaaa tccattttat
    12121 tgggaatatg gtactctgat gccaatagag caatgtcgtt attcaccaca aacgcttttg
    12181 tttgataagc ctttaataca ttattttcaa accaatcatt taatcggtct gattgttctt
    12241 tgtctttgcg ggctttcagc aaaatacctg ttttgatttc agataacgta atacagctga
    12301 tattggtctg gctaggatta acccctctca gccactggcg aacattgaca tccatcttgt
    12361 catttcttaa cttataaagc tcggaaacta tattggtatc aaaaaggtag cgatacatta
    12421 ctcatctcca aaatcaacag gtcttggttt attgcttttg cggatttcaa ccaatgtttg
    12481 ttcaaactct tgccaaaatt catcgctcac atcctcattt tcggaagagg cgaatagctc
    12541 ttcaaagctt ggctcgctag gctttttctg ctgataatcc atataactag acaccacatt
    12601 gacgattttg ccccatttgg taataaaaac cggctctttt tctgccattt ttaacacttt
    12661 gctggcattt tgattaaatt ctctggaagt aatggttatt gccatatcgc tctccaaaat
    12721 gtagtaaaaa gatgagt
//
LOCUS       DANLUQ010000063        12674 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.063, whole genome shotgun sequence.
ACCESSION   DANLUQ010000063 DANLUQ010000000
VERSION     DANLUQ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12674
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.063"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            6..1088
                     /gene="serC"
                     /locus_tag="PXB27_001363"
     CDS             6..1088
                     /gene="serC"
                     /locus_tag="PXB27_001363"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HDL5346145.1"
                     /translation="MTEIYNFSAGPAMMPKKVLEKAQQELLNWLDQGTSVMEVSHRGK
                     LFMELAAQSEADLRKLYNVPDNYRILFLQGGARGQFAAIPMNLIGKKGKALYLNSGHW
                     SATAAKEARNFAEIDEINILETEPQLKVSRLDFSDIAEQYDYIHYCTNETISGVEIFD
                     IPNVGNTPLVADMSSNILARGIDINKFGVIYAGAQKNLGPAGITIVIIRDDLIGNARK
                     DTPSIWNYATQRDADSMINTPPTFAWYLCSLVFQHLLAEGGLKATEERNLAKATLLYE
                     YLDSSTFYYNTVAHENRSLMNVTFTTGNDELNAKFVAEATACGLQALKGHKVLGGMRA
                     SIYNAMPIDGVKALIEFMKVFEQRNA"
     gene            complement(1145..2122)
                     /locus_tag="PXB27_001364"
     CDS             complement(1145..2122)
                     /locus_tag="PXB27_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HDL5346146.1"
                     /translation="MTLAVAAIIGGLIILVWSADRFVEGAASTARHFGMTPLLIGIVV
                     IGFGTSAPEMLVSASSALSGSPGIALGNAYGSNITNIALILGLTAIIKPLSVNSDVLK
                     KELPILLAVTLLSAYLVYDANVTQLDAIILLAVFFVYMAWTIITAMKSKNDALSLDVD
                     AELTNSAKMGLGKSILWLIIGLVSLVISSQFLVWGAVEVAKFFGVSDLVIGLTIVAVG
                     TSLPELASSIAAARKGEVDLALGNIIGSNLFNTLAVVGIAGAIEPMQVSAEVFSRDVL
                     MMSALTFLLFIFGINLYRRTEGGRINRLEGLLLFAAYVGYNFYLFKTAG"
     gene            2234..4801
                     /gene="glnD"
                     /locus_tag="PXB27_001365"
     CDS             2234..4801
                     /gene="glnD"
                     /locus_tag="PXB27_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HDL5346147.1"
                     /translation="MMKNSNELKTLLSAFTEQQRQAFQSENVLSLLAQRSQFYDQLLQ
                     ALWQDFGLAQYEHITLLAVGGYGREEMFPLSDLDILVLTEKPLEEAIQQRLNELFALL
                     WDSKLQLGTSVRTLEECIQIGKAEISVATNMLEGRFLLGNKSLWLRLKEALYQPDFWD
                     IKAFFRAKMAEKNERYARYHNTSYNLEPDLKHSPGGLRDLHLISWIMLRHYGVSSFVD
                     LLNKGLLYPEEYKELIAAQAVLFRMRFALHLQLKRYDNRLRFDRQLQLSEILGYQGEA
                     NQAVELMMRDYFQATKSISQLSQLLLNSFEQAYVNELQKNAQKQPLDQYFYRQEQQLF
                     VVERKIFKTQPTTMLDLFFHLTNDPTLIANADTLRQLRLCLQQQHEPMCFLPEMREKF
                     VQLFAQPKVISRAIVPMHQLGFLSAYLPQWKGIEGLMQFDLFHIYTVDEHTVRVMLNL
                     ENFSEDPSRAFFPLCHQLFATLSEKPLLYLAALFHDIGKGREGEHAQVGALDMREFAE
                     LHGFSQAQINDMVWLVAEHLTMSITAQRRDIHDPQIISEFAKKVQNQTALSSLLCLTV
                     ADICATSESLWNDWKASLFTQLYQFTLQQLAQNLNYQAVAQEHRLQALELMKFVLSAD
                     ERIALNAFWQPCPERYFLHHTPTQLVWHALHYVKTRRLPMVLISNEYARGGTEIFVCC
                     QDQPQLFARIAQLLSQKRISIHDAQIITTENGVVLDSFIVSERDGDDDLTDERCMQIQ
                     QAMMKMLSLTETGIKFTKKPVKHLSFKRKTRLRFLPPSSKQQTEFELFTLDREGLLAQ
                     IGYIFNRLRLNLINAKITTIGERVEDFFVVSNDQGKALSEQEQLELKAILITELDSE"
     gene            complement(4978..5736)
                     /locus_tag="PXB27_001366"
     CDS             complement(4978..5736)
                     /locus_tag="PXB27_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005567524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL5346148.1"
                     /translation="MITVKNLHHKIGNATILNNINLTIPNGGITALIGANGAGKSTLL
                     SLIARLQPIQQGEIHLNELNIANTASQTIARQLAILTQDNVIHSRITVKDLLLFGRYP
                     HHQGRITSHDNAIVEEALVRFDLSTFSHRFLNELSGGQRQRALIAMTFCQQTDHILLD
                     EPLNNLDMFHARELMKLLRKLTDELALTTIMVVHDINIAAAYADTIIAMKDGGIKMIG
                     TPKEIITTENLKTVFNLDAEVLECQGKPLVIHHI"
     gene            complement(5756..6632)
                     /locus_tag="PXB27_001367"
                     /pseudo
     CDS             complement(5756..6632)
                     /locus_tag="PXB27_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
     gene            complement(6685..7653)
                     /locus_tag="PXB27_001368"
     CDS             complement(6685..7653)
                     /locus_tag="PXB27_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL5346149.1"
                     /translation="MFSFFRLNLFLLILLIPISASVGVADFHWSEIFHSSEQSQIFFI
                     SRLPRTLAVILVGATLAVAGMVLQIVLKNRFIEPSMIGTSQSAAIGILLASLLFPTSA
                     LLLKMSIATLSALMGMGLFMLLIRQLPPHQQLMLPLIGIVFGNVIEAISTFIAYETDS
                     LQVLSIWFSGDFSGILAGRYELLWITAILAVVVYVMADQLTIAGLGENISTNLGINYK
                     QMTWLALIVVAMITAIVVVTVGQIPFIGLVVPNIVSRIAGDRLRKNLPAVVVLGANLV
                     LICDILGRAINAPYEVPISTIFGIIGTVIFLYLLLKEKRAEEKARG"
     gene            complement(7747..8646)
                     /locus_tag="PXB27_001369"
     CDS             complement(7747..8646)
                     /locus_tag="PXB27_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005700892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL5346150.1"
                     /translation="MIKKALFALTATAVFAASAFAKDVTIPTARGEVTFKETPSKIAV
                     FDTGSLDTLQALGVKIDGMPEFKVLSYLKPSADKAVNVGTVFEPDLEALNSLNPDLII
                     VGTRTAKKFDEVSQIAKTIDMTDNGDKLIESGLQRLDSFGKLFNKEAETEKLKAEINT
                     LLDQTKAAVKGKGNGLIILVNGGKISAFGSGYRLSWIHDNLGIPMADSSIKNTGHGQP
                     VSFEFIQKLNPDWLFVLDRIAAIGQEGQSAQQVLDNELVRQTKAWKNGNIVYLSAASY
                     LAPGGYEQMKTDLTNIKTAFEKK"
     gene            8820..10283
                     /locus_tag="PXB27_001370"
     CDS             8820..10283
                     /locus_tag="PXB27_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDL5346151.1"
                     /translation="MAEYSGTTQEKMKDKQVEQINISPHSIEAEQAVLGGIMLSNDHW
                     DNVAERVQPHDFYNYAHRIIFEQMAELVRHNKPIDIITLDQALKDKGVLQDVGGFAYL
                     AELSKNTPSAANILAYADIVRDRSDLRSVISAGNQIAEMGYKTKGRNSKEVLDEAERI
                     VFEIAEKRNNANEGPQRIDDILLKTLARMDTLSKNRHNGGVTGVSTGFVDLNKKTAGL
                     QPSDLIIVAARPSMGKTTFAMNLCENASLVDIEDKDDLDEQGNPKKKPAKPVLIFSLE
                     MPADQIMMRMLASLSRVDQTKIRTGQISDDEDTAKISSTMAILQERNNIYIDDSSGLT
                     PTELRSRARRIYRENGGLSLIMVDYLQLMRAPGFADNRTLEIAEISRSLKALAKELEV
                     PVVALSQLNRSLEQRADKRPVNSDLRESGSIEQDADLIMFIYRDEVYNDNSDLKGIAE
                     IIIGKQRNGPIGRVRLTFQGQYSRFDNYTGGHYDDEY"
     gene            10273..10578
                     /locus_tag="PXB27_001371"
     CDS             10273..10578
                     /locus_tag="PXB27_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HDL5346152.1"
                     /translation="MNTKPTQPITLCAIYRSKAKEGMYLYVPKRDQFEQVPETLRQLF
                     GKPEFVMMFNLTGEKPLVRAKNEEVLQLLIEQGYYLQMPPPPENLHKAFIAQQKGEK"
     gene            10578..11027
                     /gene="nrdR"
                     /locus_tag="PXB27_001372"
     CDS             10578..11027
                     /gene="nrdR"
                     /locus_tag="PXB27_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HDL5346153.1"
                     /translation="MRCPFCSTEETKVIDSRLAASGYQIRRRRECISCKERFTTFESA
                     ELVVPYVIKNNGSREPFDAEKLRTGLRRALNKRPVSADDVEKAISQIIIQLQAMGERE
                     VPSRHIGTLAMNALKQLDKVAYIRFASIYLSFSDIEEFIKEIEKLKE"
     gene            complement(11101..11310)
                     /locus_tag="PXB27_001373"
     CDS             complement(11101..11310)
                     /locus_tag="PXB27_001373"
                     /inference="COORDINATES: protein motif:HMM:NF015431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL5346154.1"
                     /translation="MPLNIWQKAVALLADSTVDRLVAENGLVEVLGETENPKFDLTMI
                     YAAHRYPSSIVRAFVGFVLEQVKAN"
     gene            complement(11252..11962)
                     /locus_tag="PXB27_001374"
     CDS             complement(11252..11962)
                     /locus_tag="PXB27_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL5346155.1"
                     /translation="MNLNTLRLFVAVIQHGSLSKASERLNVPIATISRQIADLEKELN
                     IQLFDRQKSGVKPTMAGQRLYEQVHLSIEQLATAKEVLFEEQGNLKGKLRLSASPACE
                     PVLAWIADFQAAYPQVQVHCTLTDRLLDLSADGIDVAFRIGQLHGEQFIAKKVGQIGS
                     KWVAHPKFLARFGTPSTVKDLKNFPIAAWAKNEASVITLGTGRQAVEIPYLFSSNDSY
                     AIEYMAKSSGSACRQHGG"
     gene            complement(11959..>12674)
                     /locus_tag="PXB27_001375"
     CDS             complement(11959..>12674)
                     /locus_tag="PXB27_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346156.1"
                     /translation="EVTINDEQWLVLERHDDFRELAKFGQKETYDRQMWLQIRSYLVK
                     ESQFSKAKNWLAQQDFMERWMPEGNEHYGMNIHQFFAENTLNEWQEIYRHRENKSPSF
                     KVIPSVDSHTWESTYGNDGISFYAPIPMLYHGMKMKNAIKDGYWVDLQNNLVCFNPEI
                     NEKFQNSLLVKKDKFLDFLKEHKLKLVWTVLGEKHAYKESKFLQELSGVYYLYGKNQQ
                     VQGSIKHFIQEIEHWGEKQ"
BASE COUNT         4050 a         2514 c         2552 g         3558 t
ORIGIN      
        1 gaaaaatgac agagatatat aacttcagtg ccggcccggc aatgatgccg aaaaaagtac
       61 tcgaaaaagc gcaacaagaa ttattaaatt ggctagacca aggcacatca gtgatggaag
      121 tcagccaccg tggaaagcta tttatggagc tggcggcaca atctgaagca gatttacgca
      181 agctgtataa tgtgccggac aattatcgta ttctatttct tcaaggtggt gcaagggggc
      241 aatttgcagc cattccaatg aatttaatcg gtaaaaaagg caaagcgctt taccttaatt
      301 cagggcattg gtcggcaaca gcagcaaaag aagcacgtaa ctttgcggaa attgatgaaa
      361 tcaatatttt agagactgaa ccgcaactta aagtgagtcg tttagatttc agcgatattg
      421 cagagcaata tgattatatc cattattgca ccaatgaaac catcagtggg gtagaaattt
      481 tcgatattcc gaatgtcgga aataccccgt tagtggcaga tatgtcgtct aatatcttag
      541 cccgagggat tgatatcaac aaatttggag taatttatgc cggagcgcaa aaaaatctag
      601 ggcctgctgg cattacgatt gtgattattc gtgatgattt aattggcaat gcacgcaaag
      661 ataccccatc tatttggaat tatgcaactc agcgagatgc tgattcgatg attaataccc
      721 cgccaacttt tgcttggtat ttatgttctt tagtgttcca acatttactg gcagagggtg
      781 gtttgaaagc tactgaagaa cgtaatcttg ctaaagctac attgctttat gagtatttgg
      841 atagttcaac attctattac aatacggtag cacacgaaaa ccgttcctta atgaatgtaa
      901 cctttaccac gggtaatgac gagctaaatg caaaatttgt ggcagaagcg accgcttgtg
      961 gtttacaggc attgaaaggc cataaagtat taggtggaat gcgtgcctca atttataacg
     1021 caatgccgat tgatggggta aaagcactca ttgaatttat gaaggtattt gaacaacgta
     1081 atgcttaatt caagcttaag aatataaatt aaagagccac gtttgtggct ctttgaattt
     1141 ggcactagcc cgcagtttta aacaaataga aattatagcc tacataagcg gcaaagagta
     1201 ataagccctc taaccgatta attctgccac cttctgtccg acgatagagg ttgatcccga
     1261 aaatgaataa taagaaggtg agagccgaca tcatcaagac atcgcgagaa aacacttccg
     1321 ctgaaacttg cattggttct atagcaccgg caatccccac cacggctaac gtattaaata
     1381 agttagaacc gataatgtta cctaaagcta aatccacttc tcctttacga gcagctgcaa
     1441 tagaagaggc taattcaggt agtgaggtgc ctaccgccac aatagtaagc ccgattacca
     1501 aatcactcac accaaaaaat ttagccactt caactgctcc ccagactaag aattgggaac
     1561 taattactag cgatactaaa ccaataatta gccaaagaat ggattttcct aatcccatct
     1621 tagcactatt ggttaattct gcatcaacat ctaagcttaa tgcatcattt ttacttttca
     1681 ttgcggtaat aattgtccac gccatatata caaaaaacac agcaagtaaa ataatcgcat
     1741 caagttgagt gacgtttgca tcataaacca aataagcaga aagcagggtg acagccagta
     1801 aaatcggcaa ctcttttttt aacacatcgg aattaactga tagtggttta attattgcgg
     1861 ttaaacctaa aatcagcgca atattggtaa tgttagaacc gtaggcatta ccgagagcga
     1921 tacccggcga tccgcttaat gcagaagatg cagatacgag catctccggt gcagatgtac
     1981 caaaaccaat gaccacaata ccgattaaca atggtgtcat accaaaatgg cgagccgtag
     2041 aggctgcccc ttccacaaaa cgatccgcac tccaaactaa tataattaag ccaccgataa
     2101 tagcggcaac agcaagtgtc ataaactatc cttttagatg aaaaacataa aggcattata
     2161 agcattttag ccaaaataaa tataggcaaa cgaaaataaa tgacctatta ttttgccagt
     2221 ttaggaagag attatgatga aaaacagcaa cgaattaaaa acgcttttat cagcatttac
     2281 ggagcaacag cggcaagcat ttcaatccga aaatgtactc agcttacttg cacaacgtag
     2341 ccaattttat gaccaactac tgcaagcact ttggcaagat tttggactcg ctcaatatga
     2401 acatattacc ctattagcgg taggggggta cggacgtgag gagatgtttc cgctttcgga
     2461 tctggatatc ttagtgttaa ctgaaaaacc gctagaagag gctattcagc agcgtttaaa
     2521 cgaactattt gccttgcttt gggacagcaa actgcaactt ggcaccagtg tgcgtacgct
     2581 cgaagaatgt attcagattg ggaaagcaga aatttcggtt gccactaata tgttggaagg
     2641 gcgattttta ttgggtaata aatcgctttg gctaagatta aaagaagcac tttatcagcc
     2701 ggatttctgg gatattaaag cgttttttag agcgaaaatg gcggaaaaaa atgagcgtta
     2761 tgcacgttat cataatacta gctataacct tgagccagat ctcaaacata gcccgggagg
     2821 attgcgagat ctgcatttaa tctcttggat tatgctacgg cattatggtg tatcttcttt
     2881 tgtcgatctc ctcaataaag ggctacttta cccggaagaa tataaggagc tgatcgcagc
     2941 acaagcggta ctttttcgga tgcgttttgc acttcatttg caactgaaac gttacgataa
     3001 tcgccttcgg tttgacagac aattacaact gagtgaaata ttaggctacc aaggcgaagc
     3061 aaatcaagcg gtggaattaa tgatgcgaga ttattttcaa gccacaaaat ctatttcgca
     3121 actgagccaa ttattgttaa acagttttga acaagcctat gtaaacgagt tgcaaaaaaa
     3181 tgcacaaaaa caaccgcttg atcagtattt ttatcggcaa gaacagcaat tgtttgtggt
     3241 cgaacgcaaa atatttaaaa cccaacccac aacaatgttg gatctctttt ttcatcttac
     3301 taatgaccct acgttaatag cgaatgccga caccttacgt cagttgagac tgtgtttaca
     3361 gcaacaacac gagcctatgt gctttttgcc tgaaatgcga gagaaattcg tacagctttt
     3421 tgctcaaccg aaagtcataa gccgagcaat agttccaatg caccaactgg gttttttaag
     3481 tgcttatcta ccgcaatgga aggggatcga agggctaatg caattcgacc tattccatat
     3541 ttatacagtc gatgagcata cggtcagagt catgttaaat ttagaaaatt ttagcgaaga
     3601 tccaagtcgg gcgtttttcc cactatgtca tcagctattt gcaacgcttt ctgagaaacc
     3661 gttgctctat cttgcggctc ttttccacga tattggcaag ggtagagagg gcgaacacgc
     3721 tcaagttggt gcgctggata tgcgagaatt tgcggaacta cacggttttt ctcaagcaca
     3781 aatcaatgat atggtttggc tggtggcaga gcatttgaca atgtcgatca ccgcacaaag
     3841 acgagatatc cacgatccgc aaatcatcag cgaatttgca aaaaaagtgc aaaatcagac
     3901 cgctttatct tcactacttt gtttaacggt tgcagatatt tgtgcaacca gtgagagcct
     3961 ctggaatgat tggaaagcct cgctgtttac ccaattatat caatttacgc tacagcaact
     4021 ggctcaaaat ttgaattatc aagcagtggc acaagagcat cgcttgcaag cattagaact
     4081 gatgaagttc gtgctttcag ctgatgagcg aatagcatta aacgcatttt ggcaaccttg
     4141 cccggagcgt tattttcttc accatacacc gactcagttg gtatggcacg ctctgcatta
     4201 tgtaaaaact cgccggttgc cgatggtttt aatcagtaat gaatatgccc gtggtggtac
     4261 agaaattttt gtatgttgcc aagatcaacc tcaactgttt gctcgaatcg cccaattact
     4321 cagccaaaaa cgcattagca ttcacgatgc acaaattatt actactgaaa atggtgtagt
     4381 gttagacagc tttattgtaa gtgagcgtga tggtgatgat gatttaaccg atgagagatg
     4441 tatgcaaatt cagcaggcga tgatgaaaat gttaagctta actgaaacag gcataaaatt
     4501 taccaaaaaa ccggtgaaac atttgtcatt taagcgtaaa actcggctgc gttttcttcc
     4561 tccgtcatca aagcaacaaa ccgaatttga actttttacc ttggatagag aagggttgtt
     4621 ggcacaaata ggctatattt ttaaccggct ccgattaaat ttaattaatg cgaaaattac
     4681 gactattggt gaaagggtag aggatttctt tgtggtcagt aatgatcaag gaaaagcttt
     4741 atcggaacaa gaacagttag aactaaaagc cattctaatt accgaactag atagtgaatg
     4801 aaaacaaccg cttggagtga agactgcaag cggtaaattt ttcaacgttt taggtaaatt
     4861 aaatcacgag ttaagagagg ttgagaattc aagaaattag aaactaaata taagtttcta
     4921 tcgtttagat taataaatta agacgcaatt tttattaatt accatgatct tcaaacgtta
     4981 aatatgatga ataactaaag gtttgccttg gcattctaac acttcggcat caagattaaa
     5041 cactgttttt aaattttcag tggtgataat ctcttttggg gtgccgatca tcttaattcc
     5101 accatctttc attgcaataa tagtatctgc gtaagcagca gcaatgttga tgtcgtgtac
     5161 caccattata gtagttaagg cgagttcatc ggttaatttt cgtaataatt tcattaattc
     5221 ccgagcatgg aacatatcta aattattcag tggttcatct aataagatat gatcggtttg
     5281 ctgacagaag gtcattgcaa ttaatgcccg ctggcgttgc cctccggata attcatttaa
     5341 gaatcggtgg gagaaagtcg ataaatcaaa tcgtaccaga gcctcttcta caatggcatt
     5401 atcgtgcgaa gtgattctgc cttgatgatg agggtaacgc ccaaataaca gcagatcttt
     5461 gacggtaata cggctatgaa tcacattatc ttgggtcaag atagccagtt gtcgagcgat
     5521 agtttgagaa gctgtgttgg ctatattcag ctcatttaag tggatttcac cctgttggat
     5581 aggttgcaag cgtgcaatta aagagagcag ggtggatttg cccgcaccat ttgctccgat
     5641 taaagcggta ataccaccat ttggaatggt taaattgata ttattcagaa tcgtggcatt
     5701 cccgattttg tggtgaagat ttttaaccgt tatcattttc ttccttaatt tatggttatt
     5761 ttttccgttt taaaatgaga taaataaaga caataccgcc aataaattca ataacgacac
     5821 tcagaacccc ttgcattttc agcacttgct cgaagattgc ctgccctaaa attaaggtga
     5881 tggcagagat taaaaaagtc attggtattc gcacgctatg gcaaacagtc gggctaattg
     5941 cattaacaat ggcacaaaca agtagcccta gaaatagaat cgggccaaca agtgcggttg
     6001 aaacagacac caacaatcca caacagatta gtaattgacg tgaaaattga tgatattcta
     6061 acccaagccc aattgcctta tctttgccaa gtaacaaaat atctaattta tgccgttgta
     6121 accagaccca aatggcagag agaatagcga tacagcctcc aatccaaagt aatgtggggg
     6181 tagtcaggtt aaatgaggca aagaagagcc ttgagcaacg gcaaactctg ttgggtcgac
     6241 attcgttgca acaaattatt taggcttcta aataagacac caaagatgac accgattaaa
     6301 agcattcggg ttaaatcgga tcgatttcga ttcagtgaac taaatagtaa tattgatgcc
     6361 cctagcatta acaaggtttc aaagaaaaac ttatagcttg ggtctaactg agtaaagcct
     6421 aaaccgccaa ataaaaatac taacgaggtt tgcaatagca agtagagcga atcgaacccc
     6481 aaaatacttg gagttaaaat aggattatgt gtgagtgttt ggaatagcag ggtcgaaata
     6541 ccaatcgtat aagcaatagt acagagcaaa ataagctttt tgccacgcag ttgcaatata
     6601 aagtcccaat tacctttcgc attataggtc atataaaaca gaatacttgc taataagatt
     6661 aagcttaaaa cgataaagcg tgatctaacc acgagccttc tcctcagccc gtttctcttt
     6721 gagcaataaa tagaggaaaa tcactgtccc aataatccca aaaatagtag aaatcggcac
     6781 ttcataaggg gcattgatgg ctcgccctaa aatgtcacaa atcaggacta aattcgcacc
     6841 taacaccacg accgcaggca gatttttacg caatcggtca cctgctatgc gagagacaat
     6901 attagggacg accaagccga taaacggaat ctgtccaacc gtcaccacga caatagcggt
     6961 aatcatcgcc accacaatta aggcaagcca agtcatttgt ttgtagttaa tgcctaagtt
     7021 ggtactaata ttttcaccaa ggcctgcaat ggttaattga tctgccatca cataaaccac
     7081 aacagccaga atagcggtaa tccagagcag ttcataccga cctgccaaaa taccggagaa
     7141 gtcgccggaa aaccaaatcg aaagcacttg taggctgtca gtttcataag caataaaagt
     7201 gctgatagct tcaataacat tgccaaaaac gatcccgatt aatgggagca ttaattgttg
     7261 atgaggcggt aattgacgaa tgagcaacat aaaaagcccc attcccatta atgcacttaa
     7321 tgtggcaata gacattttta acagtaatgc tgatgttggg aaaagtaaac tagcaagtaa
     7381 aataccaatt gccgcacttt ggctagtgcc aatcatacta ggctcaataa agcggttctt
     7441 cagcacaatt tgtaatacca tacctgcaac ggctaaggtt gcaccgacta atatcactgc
     7501 gagcgttctt ggtaaacggc tgataaaaaa gatctgactt tgttcggaag aatgaaaaat
     7561 ttcactccaa tgaaaatctg ccacacccac actggcactg attggaatga gcaatattaa
     7621 aagaaataga tttaatctaa aaaaagagaa catagtgata aatagcaagc ggtcatattc
     7681 tctctctttt ttacaaatgc aggcacttgc aaaaatagga ggaaatctga ccgcttgtaa
     7741 ggttaattat tttttctcaa atgctgtttt aatgttagtt aggtcggttt tcatctgctc
     7801 ataaccaccc ggcgctaaat atgaggcagc acttagataa acgatattac cgtttttcca
     7861 agctttagtt tgacgaacta attcattatc caacacttgt tgggctgatt gaccttcttg
     7921 tccgatagcc gcaatacggt ctaacacaaa caaccaatcc ggattgagtt tttggataaa
     7981 ttcaaacgaa acaggctgac cgtgaccggt atttttaatg ctagaatctg ccattggaat
     8041 ccctaaatta tcgtgaatcc aacttaaacg ataacctgaa ccaaatgcag aaattttgcc
     8101 accattgact aaaataatca gtccgttacc tttacctttt actgctgctt tggtttggtc
     8161 taataaagta ttaatttctg cttttagttt ttcggtttcc gcttctttat taaataattt
     8221 gccgaaagaa tcaagacgct gtaatccgct ttcgattaat ttatcgccat tatccgtcat
     8281 gtcgatggtt ttcgcaattt ggctgacttc atcaaatttt tttgctgtgc gtgtgccaac
     8341 aatgatcaaa tccggattta aagaatttaa cgcttctaaa tccggctcaa ataccgtacc
     8401 gacattcacc gctttgtctg cactcggttt aagataagaa agcaccttaa attcaggcat
     8461 accgtcaatt tttacaccta aggcttgtaa agtatccaaa cttccggtgt caaacacggc
     8521 aattttagac ggggtttctt tgaaagtaac ctctccacgt gcagttggaa tagtaacatc
     8581 tttggcaaat gcagaagcag caaacacagc agttgcggtc aatgcaaaca aggctttttt
     8641 aatcattgga taactccatt tatattgaaa ataattctca aagattttaa cgaaaattga
     8701 ttttaagtaa agcggaaaat aacaagcggg cgaattttgc ctaaaatttg caaatttcaa
     8761 tttaattaaa attccgatac actatgtaca tcaatacgtc attatagcaa gagaaaaata
     8821 tggcagaata tagcggaaca acccaagaga agatgaaaga taagcaggtt gagcaaatta
     8881 atatctcacc acattcgatt gaggcagagc aagcggtgct tggtggcatt atgctcagta
     8941 atgatcactg ggataatgtt gctgagcgtg tacaaccgca cgatttttac aactatgcgc
     9001 atcgaattat ttttgaacaa atggcggagt tggtgcgcca caataaaccg attgatatta
     9061 ttacgctgga tcaagcctta aaagacaaag gggtgctgca agatgtagga ggttttgcct
     9121 atttagccga gctttctaaa aataccccga gtgcagcaaa tattttagcc tatgcggata
     9181 ttgtgcgaga tcgttccgat ttaagaagtg taatcagtgc cggcaatcaa attgcggaaa
     9241 tgggctacaa aacgaaaggg cgtaactcca aagaagtgtt agacgaggca gagcgaattg
     9301 tgtttgaaat tgcagaaaaa cgcaataacg ccaatgaagg tccacaacgt attgacgata
     9361 ttttgcttaa aacactcgct cgaatggata cgctatctaa aaatcgccat aacggcggag
     9421 ttactggtgt ttctaccggg tttgttgatc tcaataaaaa aaccgcaggg ttacagcctt
     9481 cggatttaat cattgtggca gcccgtcctt caatggggaa aactactttt gcaatgaact
     9541 tatgtgaaaa tgcctcatta gtggatattg aagataaaga tgatttagac gaacagggta
     9601 atccgaagaa aaaaccggca aagccagtgc taattttcag cctggagatg ccggcagacc
     9661 aaattatgat gcgtatgctt gcctcacttt ctcgggtcga tcaaaccaag atccgaaccg
     9721 gtcagatttc agatgatgaa gacactgcta aaatctccag cacaatggca attttacaag
     9781 aacgtaataa tatctatatt gatgatagct ccggtttaac accgaccgaa ttacgttctc
     9841 gagcgagacg gatttatcgt gaaaatggcg gattaagctt gattatggtg gactatctcc
     9901 aattaatgcg agcgccggga tttgcagata atcgtacttt agaaatcgca gaaatttccc
     9961 gttctttaaa ggcactagcg aaagaattag aagtgccagt agtcgctctt tctcagctaa
    10021 accggagttt ggaacaacgt gccgataaac gtccggtaaa ctcggatttg cgtgagtcag
    10081 gttctattga gcaagatgca gacttaatta tgtttattta tcgtgatgag gtctataacg
    10141 acaattccga tctgaaaggc attgcggaaa ttattatcgg aaaacaacgt aacggcccga
    10201 ttggtcgggt gcgtttaacg ttccaagggc agtattcccg ttttgataat tacacaggag
    10261 ggcattatga tgatgaatac taaaccaacc cagccaatca cactttgtgc gatttatcgc
    10321 agtaaggcaa aagaaggaat gtatttatat gtgcctaagc gtgaccaatt tgagcaagtg
    10381 ccggaaacat tacgccagtt atttggtaag cctgaatttg tgatgatgtt taatttaacc
    10441 ggtgaaaagc cattggtgcg tgctaaaaac gaagaagtgt tacaattact tatagagcaa
    10501 ggctactatt tgcaaatgcc acctccgccg gagaatttgc ataaagcctt tatcgcacaa
    10561 cagaaagggg aaaaataatg cgctgcccat tttgttccac tgaagaaact aaagtgatcg
    10621 attcacggct tgcagcaagc ggttatcaaa ttcgccgccg ccgtgaatgt ataagctgta
    10681 aagaacgttt tactaccttt gaatcggccg aattagttgt gccttatgtg atcaaaaata
    10741 atggtagccg agagccgttt gatgccgaaa aattaagaac agggttaaga cgtgcattaa
    10801 ataaacgccc tgtcagtgct gatgatgtcg aaaaagcaat tagccaaatc attattcagc
    10861 tgcaagcgat gggcgaacga gaagtgccga gtcgccatat cggtacattg gcaatgaacg
    10921 cacttaaaca attagacaaa gtagcatata tccgctttgc ctctatttat ttaagcttta
    10981 gtgatattga agagttcatt aaagaaattg aaaaattaaa agagtagttg caaaaaaaca
    11041 taaaaaaatg accgcttgtg agtttaaaat ccacaagcgg tcatttttct ttaaaaatac
    11101 tcaattagct tttacttgtt ccaacacaaa ccctacaaac gcccgcacaa tgctagacgg
    11161 ataacgatgg gcggcataaa tcatcgtcaa atcaaatttg gggttttctg tctcacccaa
    11221 gacttccacc aagccatttt ctgccaccaa tctatccacc gtgctgtcgg caagcagagc
    11281 cactgctttt tgccatatat tcaatggcat agctgtcatt gctggaaaaa agatagggaa
    11341 tttctaccgc ttgtctgcca gtgcctagcg tgatgacgct tgcttcattt tttgcccaag
    11401 cggcaatggg gaaatttttc aaatctttga cggtgctggg cgtgccaaag cgtgccaaaa
    11461 atttagggtg tgccacccat ttactgccga tttgccccac ttttttggcg ataaactgtt
    11521 cgccgtgtag ttgcccaatc cgaaacgcca catcaatgcc atctgccgac aaatccaaaa
    11581 gacggtcggt cagcgtgcaa tgcacttgta cttgtggata agctgcctga aaatcagcta
    11641 tccacgccaa aacaggctcg caggcagggc ttgccgacag gcgtaattta ccctttaaat
    11701 tgccttgctc ttcaaacaac acttctttgg cagtggcaag ctgttcaatg gacaaatgca
    11761 cctgttcata caggcgttgc cctgccattg tgggtttaac gcctgatttt tggcggtcaa
    11821 agagttgaat gttaagctct ttttccaaat cggcaatttg acggctaatg gttgcaatcg
    11881 gcacattgag tcgctcggag gctttggaga gactgccgtg ctggataacg gcgacaaaga
    11941 ggcggagggt gttgaggttc attgtttttc tccccaatgt tcaatttctt gaataaaatg
    12001 tttaatactg ccttggactt gttgattttt accatataga taataaacgc ctgaaagttc
    12061 ttgtaaaaat ttactttctt tataagcgtg tttttcaccc aacacagtcc aaactaactt
    12121 taacttatgc tcttttaaaa aatccaaaaa tttgtctttt ttaaccagta agctattttg
    12181 aaatttttca tttatctctg gattaaagca aaccaaatta ttttgtaaat caacccaata
    12241 accatcttta atcgcatttt tcattttcat tccgtgataa agcataggaa ttggcgcata
    12301 aaatgaaatg ccgtcattgc cataagtaga ttcccaagtg tgagaatcga cactaggtat
    12361 taccttaaat gaaggcgatt tattttccct atgtcgataa atttcttgcc actcatttaa
    12421 tgtattttcg gcaaaaaatt gatgaatatt cattccatag tgttcattac cttctggcat
    12481 ccatctttcc ataaaatctt gttgtgccaa ccaatttttt gcttttgaaa attgagattc
    12541 tttaactaaa taacttctaa tttgtaacca catttgccta tcataagttt ctttttgccc
    12601 aaattttgct aattcacgaa aatcatcgtg tctttctaga acaagccact gttcatcatt
    12661 aatcgttact tcca
//
LOCUS       DANLUQ010000064        12635 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.064, whole genome shotgun sequence.
ACCESSION   DANLUQ010000064 DANLUQ010000000
VERSION     DANLUQ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12635
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.064"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            11..1048
                     /locus_tag="PXB27_001376"
     CDS             11..1048
                     /locus_tag="PXB27_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HDL5346157.1"
                     /translation="MHNKLLSICILRLSAIGDVCHTLAVIQAIQRKYPSAEITWIIGK
                     TEAYLMKDIPNVHLIPYDKKIGWKGIFTLWQKLSHKRFDFLLNLQTAFRASILSLGIK
                     AKKKIGFNQDRAREMQWLFTNQKVEMTDSPHVLDGQMMFAKAIGIMDLSAQWNLPVSD
                     EDLAYGKQFIDFTRKNILIAPCSSKKEKDWLPERYAEIANWLSKQNINVLIAGSPSQY
                     EMETAAKIQQLAPNCTSIAGKTTLKQLAGVIRQVDLVISSDSGPAHIATTQNTPVIGL
                     YAIHNPRRTGPYNDLDKVVSVYDEAILQSYGKPWQQLAWATKAKGKNWMEKITVESVK
                     QKVVETLKITL"
     gene            1088..2146
                     /locus_tag="PXB27_001377"
     CDS             1088..2146
                     /locus_tag="PXB27_001377"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDL5346158.1"
                     /translation="MNTVFNQDSVHNVNIQDEKVLLTPQELKQEFPLPEHLRNQIERS
                     RKTISDIIHRRDKRQLIVIGPCSIHDPVAALEYGKKLKALSEQVSDKLYIVMRVYFEK
                     PRTTVGWKGLINDPKMDGSFDVETGLRIARKLCLDLAELGLPLATEALDPMTPQYLAD
                     LFSWSAIGARTTESQTHRELASGLSMAVGFKNGTDGDLAVALNAMQSSAQSHSFIGIN
                     QQGQVTLLKTKGNADGHIILRGGKTPNYEKQYVDDCEWVLKQANLPTAIMIDCSHGNS
                     NKDYRRQPLVAENVLEQLLAGNTSIIGLMIESNLHAGNQSSELPFEQMKYGVSITDAC
                     IDWQTTEELLTHFAEKLR"
     gene            2201..3322
                     /gene="tyrA"
                     /locus_tag="PXB27_001378"
     CDS             2201..3322
                     /gene="tyrA"
                     /locus_tag="PXB27_001378"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HDL5346159.1"
                     /translation="MNPLAPLREQIDQVDQQLIALLSERLALVAEVGKVKSEHGIPVY
                     APERETAMISARRSEAEKQGIPADLIEDVLRRIMRESYANENKHGFKRVNSDIQKIVI
                     VGGKGKLGGLFGNYLTLSGYNVEALGRDDWDNAEQILANADVVIVCVPIANTLATIER
                     LQSYLTENMILADLTSVKHQPLQKMLEVHNGAVVGLHPMFGPDIASQAKQVVACCHGR
                     FPERYQWLLEQIKIWGAKIEEIEAAAHDNAMTYIQALRHFSTFAFGLHLSRQPVQLSQ
                     LLALSSPIYRLELAMIGRLFAQDGSLYADIIADKPENLATIETLKESFEQGLDFFKRN
                     DKAGFIKAFEEVHHWFGDYSEQFLKESRVLLQQAHDSRK"
     gene            complement(3513..4598)
                     /gene="recF"
                     /locus_tag="PXB27_001379"
     CDS             complement(3513..4598)
                     /gene="recF"
                     /locus_tag="PXB27_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDL5346160.1"
                     /translation="MALTRLLINHFRNIQHTDLAFSPHFNFLVGANGSGKTSLLESIF
                     YLGHGRSFKSHISNRIIHYDKDDFVLHGKIDEAKHSWSVGIQKFRSGETTLKINGEDG
                     NKIADLAHLLPMQVITPEGLTLLNGGPSFRRAFLDWGLFHQHPDFYAHWNNLRRLLKQ
                     RNSALQQVRSYQELKAWDIELVKTTYAVSEMRAEYAEALRPEIEKTCQFFLPEIEIGL
                     SFHQGWEKGADYAEILAQGFERDKALGYTMIGAQKADFRFKANGLPVEDVLSRGQLKL
                     LMCALRLAQGEHLVAQKQRQCLFLIDDFASELDPTKRELLAHRLRESGSQVFVTAITQ
                     DQLNQMQWQDRSDDCLFTVKNGEIQPQ"
     gene            4738..5067
                     /locus_tag="PXB27_001380"
     CDS             4738..5067
                     /locus_tag="PXB27_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDL5346161.1"
                     /translation="MFGKGGLGGLMKQAQQMQERMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRIEIDPSLMEDDKEMVEDLVAAAFNDAVRRAEEMQKEKMASVTAGMQLP
                     PGMKFPF"
     gene            5075..5680
                     /gene="recR"
                     /locus_tag="PXB27_001381"
     CDS             5075..5680
                     /gene="recR"
                     /locus_tag="PXB27_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015939588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDL5346162.1"
                     /translation="MQTSPLLENLMEALRALPGVGPKSAQRMAYHLLQRNRSGGVALS
                     KALNEAMSHIGHCKSCRTFTEEEECTICKNPRRQMSGQLCVVEMPEDIQAIEQTGQFS
                     GRYFVLMGHLSPIDGIGPREIGLDLLQQRLEQESFHEVILATNPTIEGDATANYIAEM
                     CRMYNVKATRIAHGVPVGGSLEMVDGTTLSHSFAGRRDIDI"
     gene            5752..6078
                     /gene="grxD"
                     /locus_tag="PXB27_001382"
     CDS             5752..6078
                     /gene="grxD"
                     /locus_tag="PXB27_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005705415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HDL5346163.1"
                     /translation="METIDKIKQQISENPILLYMKGSPKFPSCGFSARAVEAVINCQV
                     PFGYVDILTNPDIRAELPKFANWPTFPQLWVEGELVGGCDIVLEMLQKGELQTLLKET
                     AAKHAA"
     gene            6220..7257
                     /locus_tag="PXB27_001383"
     CDS             6220..7257
                     /locus_tag="PXB27_001383"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HDL5346164.1"
                     /translation="MALKELDWANLGFSYIKTDYRFIAHWKDGKWNEGELTQDNTLHI
                     HEGSTALHYGQQCFEGLKAYRCKDGSINLFRPDQNAERMQRTADRLLMPRVPTELFIR
                     ACKKVVKANQDWLGPYGSGATLYLRPFLIGVGENIGVKAAPEFIFSVFCCPVGAYFKG
                     GLAPSNFITTDYDRAAPMGTGGVKVGGNYAASLLPHELAVEQSSTTRKFADAIYLDPK
                     THTKIEEVGAANFFGITKDNKFITPISESILPSITKYSLLHLAQERLGMEAIEGDVYI
                     DQLDQFAEAGACGTAAVITPVGGIQHKDKFHVFYSETEVGPVTRRLYAELTGIQFGDV
                     EAPQGWIVKVE"
     gene            7284..7832
                     /locus_tag="PXB27_001384"
     CDS             7284..7832
                     /locus_tag="PXB27_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane lipoprotein lipid attachment
                     site-containing protein"
                     /protein_id="HDL5346165.1"
                     /translation="MAKLFANSTACLYLENMKKLIFLGLSALALAGCSSTISNPNQLI
                     GQWQCTIEYDDFNIRTVDNLRFSTNGTMTNQGGIYYPIQKSIFEYSLQQNGRWSVKNG
                     AIVYRIVSESVQRSHRSQIWSELQRDAELQQFEENLFSSLSDSDNNKTVELIITDFDE
                     KEMDIKQEIKGHKAYKGQCIKQ"
     gene            7841..8185
                     /locus_tag="PXB27_001385"
     CDS             7841..8185
                     /locus_tag="PXB27_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDL5346166.1"
                     /translation="MIQRFEVGSRFSEMAIHNGVAYLAGQVPLDDTADAYTQTQQVLA
                     EIDKWLAKANSDKSKILMATVYLKEMADYAEMNRAWDEWVAPNNAPPRAAVEAKLANP
                     NWKVEIVVTAAV"
     gene            complement(8218..9996)
                     /locus_tag="PXB27_001386"
     CDS             complement(8218..9996)
                     /locus_tag="PXB27_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HDL5346167.1"
                     /translation="MSTLDFHFIHRFHQQVQALSHHPALRFMQDEKWSEISWLELQKQ
                     VNNLSYALLANGLAVQEKIAIFAQNMPRWTITDIAAMQIRAVAVPIYATSTTKQLEYI
                     LNDGDIKILFVGDQEQYDQAIEIANNCSLLIKIVAMKDSIDLRDFPIAQHWQDFIKVN
                     QDEAELTKRLNDKCLEDLFTLIYTSGTTGEPKGVMLDYANLAHQLQAHNKALPHIDEN
                     DISLSFLPLSHIFERAWVTYVLYRGAVNCYLEDPQQVRQALSTVRPTLMCAVPRFYEK
                     IYTAVWDKVQQAPIHRRALFKWAIRTAEDNLFGKKSAFFHRLQYIMADKLVLGKLRAL
                     LGGRIRMMPCGGAKLDPSIGLFFQSIGINVKLGYGMTETTATISCWQEKGFNINSTGT
                     LMPDVEVKISEENEILVRGGLVMKGYYKKPEATAQAFTAEGFLKTGDAGELDSEGNLY
                     ITDRIKELMKTSNGKYIAPQYIEGKIGKDKFIEQIAVIADAKKYVSALIVPCFDSLEE
                     YAKKLNIRYQDRLELIKHYEVVRMFEQRINELQKDLASFEQIKKFTLLSQAFSTKMEE
                     ITPTLKLRRKVILERYKQQIEKMYES"
     gene            complement(10222..10791)
                     /locus_tag="PXB27_001387"
     CDS             complement(10222..10791)
                     /locus_tag="PXB27_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005821628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HDL5346168.1"
                     /translation="MNQENHKIEDIIRIFNQCFSDEYNTRLERGGNYPIYLPEFMDED
                     GVPSERPYNVILFAHGYYSSALHEIAHWLVAGEARRKLEDFGYWYEPDGRSAERQREF
                     ESAEIKPQAIEWVLATAAGFRYFASADNLSGNPGDNSAFKQAVYDQVKLYAERGYLPK
                     RAETLRKALCAFYGTPDVIDLAQFDIERI"
     gene            complement(10801..12564)
                     /locus_tag="PXB27_001388"
     CDS             complement(10801..12564)
                     /locus_tag="PXB27_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HDL5346169.1"
                     /translation="MIRYFFSLVLSLLMINSSHANLFSQPKFLKSEEAFVFSIQNENE
                     TLQLNWDIADNYYLYKKEIRVTPQNIEIGDITFPAAEQYNDEFFGQVEIYRNHLGLKV
                     PFKEIKDNASIVVEYQGCTKGFCYPPERVELKLDSILSATPEELAQLEAQAVEKSENF
                     AKTDNMAPPENVAKAEQDQLAENLANNRFSIFWFFVLGVGLAFTPCVLPMLPLLSAIV
                     IGNKQRPSTAKALLLSIAYVQGMAVTYTLLGLIVAAIGLPFQIALQSPPVLIGLSIVF
                     ILLACSMFGLYEIQLPNSWQQKLNAMSQKQQGGALGSVFLMGMIAGLIASPCTSAPLS
                     GALLYVAQSGDLVTGGLALYLLALGMGLPLILITLFGNKILPKSGEWLLKVKAAFGFV
                     MLALPVFLLSRVLPSHYEPFLWSALAVAFLIWLVDAIPSNSITKKIIKVLLLLALAIA
                     AKPWADLAWNGSTTQQQTTPHLALEKIQSLADLETKLTASKGKKVMLDLYADWCVACK
                     EFEKYTFSDQNVQNKLNEMVVLQIDMTKNSAENIELMKHFNVLGLPTILFFDENGNEM
                     SQSRVTGFLDAEQFLAWLNKL"
BASE COUNT         3791 a         2474 c         2723 g         3647 t
ORIGIN      
        1 tgatattatt atgcacaata aacttctttc tatctgtatt ctccgccttt ctgccattgg
       61 cgacgtttgc cataccttag cggttattca agctattcaa cgaaaatatc ctagtgctga
      121 aattacttgg attatcggca aaacggaagc gtatttaatg aaagatattc cgaatgttca
      181 cttaattcct tatgataaaa aaatagggtg gaaaggtatc tttacacttt ggcaaaaact
      241 ttctcacaag cggtttgatt ttttattaaa tttacaaacg gcattccgag cttcaattct
      301 atcgcttggg attaaagcaa agaaaaaaat cggctttaat caagacagag caagggaaat
      361 gcaatggttg tttaccaacc agaaagtaga aatgacagac tctcctcacg tgcttgacgg
      421 gcaaatgatg tttgccaaag ccattgggat aatggattta tcggcacaat ggaatttacc
      481 tgtgagtgat gaagatttag cttatggcaa acaatttatt gattttacaa ggaaaaatat
      541 actcattgct ccttgttcaa gcaagaaaga gaaagattgg ttgccggaac gctatgcaga
      601 aattgctaac tggctaagta agcaaaatat caatgtatta attgcaggtt ctccatcgca
      661 atatgaaatg gaaaccgcag ccaaaattca acaacttgcc ccgaattgta cgagtattgc
      721 cggtaaaacc acgctaaaac agcttgcagg tgttattcgt caggttgatt tggtgatctc
      781 ttcagattca ggtcctgccc atatcgccac tacgcaaaat acgccggtga ttgggttgta
      841 tgcgattcat aatccaagac ggacaggccc ttacaatgac ttagacaaag tggtttcggt
      901 atatgatgaa gcgattttgc aatcttatgg taaaccttgg cagcaattag cttgggcaac
      961 taaagctaaa ggcaaaaatt ggatggaaaa aatcacggtg gagagtgtga aacaaaaagt
     1021 agttgaaact ttaaaaatta cgttgtaaag tttgtgtatt aaaagattta cacaaaagga
     1081 aattaaaatg aacacagtgt ttaatcaaga tagcgtgcat aacgtaaata tccaagatga
     1141 aaaggtatta ctgaccccac aagagttaaa acaagaattt ccgttgccgg aacatttacg
     1201 caatcaaatt gaacgttcac gcaaaacgat ttccgatatt attcacagac gagataaacg
     1261 ccaactgatt gtgattggac cttgttcgat tcacgaccca gttgctgccc ttgaatatgg
     1321 taaaaaatta aaagctcttt cggagcaagt gagtgataag ttgtatattg tgatgcgggt
     1381 ttattttgaa aagccccgca ctactgtagg ttggaaaggg ctgattaacg atccgaaaat
     1441 ggacggctct tttgatgtcg aaaccggctt acgtatcgca cgaaaactct gtttggattt
     1501 agccgaactt ggcttgccgt tagcgaccga agccttagat ccgatgacac cacaatattt
     1561 agcggatttg ttcagctggt cggcaattgg agcaagaaca actgagtctc aaactcaccg
     1621 tgaattagcc tcaggtttgt caatggcggt aggctttaaa aatggtacag atggtgattt
     1681 agccgttgca cttaatgcga tgcaatcttc cgctcaaagc catagcttta tcggcattaa
     1741 ccaacagggg caagttactt tactcaaaac aaaaggtaat gcagacggac atattatttt
     1801 acgtggcggt aaaaccccaa attatgaaaa gcaatatgtt gatgattgtg aatgggtgct
     1861 aaaacaagca aacttaccta cagcgattat gattgattgc agccacggca attccaataa
     1921 agattaccgc cgccaaccgc tggtggcaga aaatgtatta gagcagctac ttgctggcaa
     1981 tacttcgatt atcggtttaa tgattgaaag caatttacac gcaggtaatc aatcatcaga
     2041 attgccgttc gagcagatga aatacggtgt ttctattacg gatgcttgta ttgactggca
     2101 aactacagaa gagcttttaa ctcactttgc tgaaaagttg cgttaaagat aatttgcaaa
     2161 aaattgcata aaaacgaccg cttgtaaatt ggtaacaaat atgaacccat tagccccatt
     2221 acgagaacaa attgatcaag ttgatcaaca acttatcgcc ttactttctg aacgcttagc
     2281 tcttgtggca gaagttggga aagtcaaatc agaacacggc attcctgttt atgctccgga
     2341 gcgagaaacc gcaatgattt ccgcccgccg ctctgaagcg gaaaaacaag gtattccggc
     2401 ggatctcatt gaagatgtgc tacgccgtat aatgcgtgaa tcttatgcca atgaaaacaa
     2461 acacggcttc aaaagagtta attcggacat tcaaaaaatc gtgattgttg gtggtaaagg
     2521 gaaattaggt gggctgtttg gcaattattt aaccctatcc ggctataatg ttgaggcatt
     2581 aggacgtgat gattgggaca atgccgagca gattttagcc aatgcagatg tggttattgt
     2641 gtgtgtgccg attgccaaca ccctagcaac catcgagcgt ttacaatctt atttaaccga
     2701 aaatatgatt ttggcggatt taacctccgt caaacatcag ccgttgcaaa aaatgctgga
     2761 agtgcataat ggagcggtag tcggtttaca cccgatgttc ggaccggata ttgcaagtca
     2821 ggcaaagcaa gtggtcgctt gctgccacgg gcgtttccct gaacgttatc aatggttgct
     2881 tgagcaaatc aaaatttggg gggcaaaaat tgaagagatt gaggcagcag cacacgataa
     2941 tgcaatgacc tacattcaag ccctacgcca cttttccact tttgcattcg ggctacatct
     3001 ttctcgccag ccggtgcaac tctctcaatt attggcacta tcttcgccga tttaccgctt
     3061 agagttggca atgattgggc gtttatttgc ccaagacggc agcttatatg ccgatattat
     3121 tgccgataaa ccggaaaatt tagccaccat cgaaacctta aaagagagtt ttgaacaagg
     3181 gctagatttc tttaaacgca acgacaaagc cggttttatc aaggcttttg aggaagtgca
     3241 tcactggttt ggcgattatt ccgagcagtt cttgaaagag agtagagtat tgctccaaca
     3301 ggctcacgat tcaagaaagt aaaggtaggt tgaggttttg ctctgatagc actagcgtcc
     3361 acgctagtgc tatttaatat tctacaagcg gttaataaag agctgttttt tgcaaatttt
     3421 tatgcaaaat tatccgcttg ttttggcacg agcgtggacg ctcgcgccat cggaagaaac
     3481 atttttgtaa aattgccccc aaatctgacc gcttactgtg gctgaatttc tccatttttt
     3541 accgtaaaca aacaatcatc gcttctgtct tgccattgca tttgattgag ctgatcttga
     3601 gtaatggcgg taacgaatac ttgtgagcca ctttctctta aacgatgtgc taaaagctca
     3661 cgtttggttg ggtcgagttc ggaggcaaaa tcgtcaatta aaaataggca ttggcgttgt
     3721 ttttgggcga ccaaatgttc gccttgagcc agtcttaacg cacacatcaa tagctttaat
     3781 tgcccacggg aaagcacatc ttccaccggt aagccgttcg ctttaaaacg gaaatcggct
     3841 ttttgagctc caatcatcgt atagccaaga gctttatccc gctcaaaacc ttgtgccaga
     3901 atctcggcat aatccgcccc tttttcccag ccttgatgaa agctcaagcc gatttcgatt
     3961 tccggcagga aaaattggca ggttttctca atttcggggc gtaacgcttc ggcgtattcg
     4021 gctcgcattt cgctcacggc ataagtggtt ttcaccaatt cgatatccca cgctttgagt
     4081 tcttgataag agcggacttg ttgcaaagcc gaattacgtt gctttaataa acggcgaaga
     4141 ttgttccaat gagcgtagaa atcagggtgc tggtgaaata agccccagtc caaaaaagca
     4201 cggcgaaagc tcggaccacc gttaagtaag gttaagcctt cgggggtgat cacttgcatc
     4261 ggcaataaat gggctaaatc ggcaattttg ttgccgtctt caccgttgat tttaagtgtc
     4321 gtctcgccac tgcgaaattt ctgaatgccg accgaccaac tgtgctttgc ctcgtcaatt
     4381 ttaccgtgta gtacgaaatc gtctttatcg taatggataa tgcggttgct gatatggctt
     4441 ttgaatgaac gcccgtgtcc aagatagaaa attgattcca acaaactggt tttgccactt
     4501 ccgttcgccc cgactaaaaa attaaaatgc gggctaaatg ctaaatcggt atgttggata
     4561 tttctaaaat ggttgattaa taaacgggtt aaagccatag ttaaagtgcg aggaataaaa
     4621 agagtagcca ttttagcaaa tttgcaaaat tttgggaaaa attaaccgct tgcggattag
     4681 aaagtggttc agtagaataa gccgtttatt ttttattgaa aattaagagg aaaaattatg
     4741 tttggaaaag gtggcttagg cggcttaatg aaacaggctc agcaaatgca agagcgtatg
     4801 caaaaaatgc aagaagagat cgcccaatta gaagtaacag gcgagtctgg tgcgggtttg
     4861 gtaaaagtca ctattaacgg ggctcacaat tgccgccgaa ttgaaattga tccatcatta
     4921 atggaagatg ataaagagat ggtggaagat ttagttgccg ctgcctttaa cgatgcggtt
     4981 cgccgtgcag aagaaatgca aaaagagaaa atggcaagtg tgactgccgg tatgcaactg
     5041 ccgccgggta tgaaattccc attctaattt aagtatgcaa accagtcctt tacttgaaaa
     5101 tttgatggaa gccttgcgtg cattgcccgg tgtcggaccg aaatcggcac agcgaatggc
     5161 ttaccatctg ttgcaacgta atcgttccgg cggtgtggcg ttgtcaaaag ccttaaatga
     5221 ggcaatgagc catatcggac attgcaaatc gtgccgtact tttaccgaag aggaagagtg
     5281 tacgatttgt aaaaatcctc gccgtcagat gagcgggcaa ctttgtgtgg tagaaatgcc
     5341 ggaagacatt caagcgattg agcaaaccgg gcaattttca gggcgttatt ttgtgctgat
     5401 ggggcatttg tcgccgattg atggtatagg ccctcgtgaa atcggcttag atttattgca
     5461 acaacgcttg gaacaggaat ctttccacga agtgattttg gcgactaatc cgaccattga
     5521 aggtgatgct accgcaaact acattgctga aatgtgcaga atgtataacg tgaaagctac
     5581 tcgtattgct cacggtgtgc cggtcggtgg ttcattagaa atggttgatg gcacaacgct
     5641 ttctcactct tttgcaggac ggcgtgatat tgatatttaa agctatttct gtgctaactt
     5701 aattctgata taattactca actattttaa attattattt tgaggctaaa aatggaaact
     5761 attgacaaaa tcaaacaaca aatcagcgaa aatccgattc tactttatat gaaaggttca
     5821 ccgaaattcc catcttgtgg tttctccgct cgtgcggtgg aagcggtaat taactgccaa
     5881 gtgcctttcg gttatgtgga tattttaacc aatccggata ttcgtgcgga attgccaaaa
     5941 tttgcaaact ggccgacttt cccacaatta tgggtagaag gtgaattagt tggcggttgt
     6001 gacatcgtat tagaaatgct ccaaaaaggc gaacttcaaa ccttattaaa agaaaccgca
     6061 gcaaaacacg cagcataatt tcaaaccagt atgttaaggc tcttatgttt aagagcctta
     6121 attttttctt gcgatttagg cttaaacttc ttacctcaag agctattttg ttttagaatg
     6181 tcaaaattat ttcgtaacat aaaaataaaa ggacacaaca tggcattaaa agagttagat
     6241 tgggcaaatt taggcttttc ttatatcaaa accgattacc gtttcattgc acattggaaa
     6301 gacggaaaat ggaatgaagg cgaattaact caagacaata cactgcatat tcacgaaggc
     6361 tcaactgccc tgcactatgg gcaacaatgt tttgaggggt taaaagccta tcgctgtaaa
     6421 gatggctcaa ttaacttatt ccgcccggat caaaatgccg agcgtatgca acgcaccgca
     6481 gatcgtttat taatgccacg tgtgccaacc gagctgttta ttcgtgcctg taaaaaagtg
     6541 gtaaaagcca atcaagattg gttagggcct tacggttcgg gggcaaccct ttatttacgt
     6601 ccgtttttaa tcggcgtggg cgaaaatatt ggggtgaaag ctgcacctga atttattttt
     6661 tccgtattct gctgtccggt gggggcatat ttcaaaggtg gcttagcacc ctcaaacttc
     6721 attacaaccg actatgaccg tgcagcccca atgggaacag gtggtgtgaa agtaggcggt
     6781 aactacgctg cgagtttatt accgcacgaa ttggcggttg agcaaagttc aactacccgt
     6841 aaatttgccg atgcgattta tttagatccg aaaacccata ccaaaattga agaagtgggg
     6901 gcggcgaact tctttgggat taccaaagac aacaaattca tcaccccgat ttcagagtcg
     6961 attttgccaa gtattaccaa atactcattg ctacaccttg ctcaagagcg tttaggtatg
     7021 gaagcgattg agggagatgt gtatatcgac cagttagacc aatttgccga agccggtgct
     7081 tgtggtacgg ctgcggtgat tacgccggtt ggtggtattc aacataaaga caaattccac
     7141 gtcttctatt cagaaaccga agtggggcct gtcacccgcc gtttatacgc agaattaacc
     7201 ggtattcaat ttggcgatgt tgaagcaccg caaggctgga ttgtaaaagt agagtaattt
     7261 tacacaagta acaagcggtc gaattggcaa aactttttgc aaattcaacc gcttgtcttt
     7321 atttagagaa tatgaaaaag ttaatttttt taggtttatc agcacttgca ttagctggtt
     7381 gttcctcaac aatttctaat cctaatcaac tgattgggca atggcaatgc actattgagt
     7441 acgatgattt taatattcgt acggtggata atttacgatt ttccacaaat gggactatga
     7501 ccaatcaagg tggtatatat taccctattc aaaagtcgat ttttgagtat tcattacaac
     7561 agaatgggcg ctggagtgtg aaaaacgggg ctattgttta tcgtatcgtc tcagaatctg
     7621 tacagcgtag ccatcgttca caaatatggt ctgaattaca acgtgatgcg gagctacaac
     7681 aatttgaaga gaatttattt agctcacttt ctgatagtga taataataaa acagtggaac
     7741 taatcatcac tgattttgat gaaaaagaaa tggatatcaa gcaagaaata aaagggcata
     7801 aagcttataa agggcaatgt ataaaacagt agaggtaaaa atgattcaac gttttgaagt
     7861 aggtagccgt ttttcggaaa tggcaataca taacggtgtg gcttacttgg cagggcaagt
     7921 gccgttagat gacaccgccg atgcttacac ccaaacccag caagtgcttg cagaaattga
     7981 taaatggctg gcaaaagcca acagcgataa aagtaaaatt ctaatggcta cggtttattt
     8041 aaaagagatg gcagattatg cggaaatgaa ccgtgcgtgg gacgaatggg ttgccccaaa
     8101 taacgctcca ccaagagcgg cggtggaggc taaattagcc aatccaaatt ggaaagtcga
     8161 aattgtggtt accgctgcgg tataaatgaa aagcagaaag acgatctctg tttttcatta
     8221 tgattcgtac attttttcga tttgttgctt atagcgttct agaatgacct ttcgtcttaa
     8281 ttttaatgta ggcgtaattt cttccatttt ggtgctaaat gcttgcgaaa gtagtgtaaa
     8341 ctttttgatc tgttcaaaac ttgcaaggtc tttttgtaat tcgttaatgc gttgttcaaa
     8401 cattctgaca acctcataat gtttaatgag ttctaatctg tcttgatagc gaatattgag
     8461 cttttttgca tactcttcga ggctatcaaa gcaaggaact atcaacgcag aaacatattt
     8521 tttggcatca gcaatcaccg caatttgctc aataaactta tctttaccta tttttccttc
     8581 aatgtattgt ggggcaatat atttaccgtt tgaagttttc attaattctt taatacgatc
     8641 ggtaatataa agattgcctt ccgaatccag ttcaccggca tctcccgttt ttagaaaacc
     8701 ttcagcagta aaggcttgag cagtggcttc cggttttttg tagtaacctt tcataaccaa
     8761 gcctccacgc actaaaattt cattttcttc actaattttg acctcaacat caggcattaa
     8821 ggtaccggtt gagttgatat taaagccttt ttcctgccag catgaaatag tggcagtagt
     8881 ttcggtcatt ccgtatccaa gttttacatt gatgcctata ctttggaaaa atagcccgat
     8941 agacggatct aatttagctc ctccacacgg catcatacga atacgaccgc ctagtaaggc
     9001 tcgtaatttt cctagcacca atttatctgc cataatgtac tgcaagcggt ggaaaaaggc
     9061 tgatttttta ccaaacagat tgtcttcggc ggtacggatt gcccatttaa ataacgctcg
     9121 gcgatgtatc ggtgcttgtt ggactttatc ccacaccgca gtatagattt tttcatagaa
     9181 ccttggtacg gcacacatta atgtaggtcg taccgttgag agtgcttgac gtacctgttg
     9241 aggatcttcc agatagcaat ttactgcccc tctgtatagc acataagtga cccaagcacg
     9301 ttcaaagata tgcgataaag gtaaaaatga aagggatata tcattttcgt caatatgagg
     9361 gagtgcttta ttatgtgcct gaagttgatg agctaaatta gcataatcta acataacacc
     9421 tttaggctca ccggttgttc ccgaggtata aattaaagtg aataaatcct ctaagcattt
     9481 atcatttaaa cgtttggtta attcagcttc atcttgatta accttaatga aatcttgcca
     9541 atgttgagcg atagggaaat cacgcagatc tattgagtct ttcatcgcga caatttttat
     9601 taatagcgaa cagttgttcg ctatttctat ggcttgatca tattgttcct gatcgccaac
     9661 aaagaggatt tttatatccc cgtcattcag gatatattca agttgtttgg tggtactggt
     9721 tgcataaatc ggcactgcta ccgcacgaat ttgcattgca gcaatatcgg taattgtcca
     9781 acgaggcata ttttgtgcaa aaatagcgat tttctcttga acggcaagac cgtttgccag
     9841 caaggcataa gaaagattat tgacctgttt ttgtaattct aaccaactta tttctgacca
     9901 tttttcatct tgcataaaac gtaaagctgg gtggtggcta agagcctgaa cttgctggtg
     9961 gaaacggtgg ataaagtgaa aatctaaagt gctcataatc ttatttttac tttctatttc
    10021 agtgaacagt tgtacgctaa tataatagaa aatatgaaaa aaagaaacag ttgtactctg
    10081 aaacaaaggg ttttggatag gaagtaatca aggcaataga aatagataag tagggtatcg
    10141 tttagtacaa atgcaatatt tttcaaaaat gagaccgctt gtatatgcaa caagcggtct
    10201 gttttgttaa aaattttgca actaaattcg ttcaatatca aattgagcta aatcaatcac
    10261 atcaggtgta ccgtaaaaag cacagagggc tttacgcaag gtttcagctc gtttgggaag
    10321 gtagccacgt tctgcataga gcttgacttg gtcgtacact gcttgtttaa aggctgaatt
    10381 atcgccggga ttgcccgaga gattgtcggc actagcaaaa taacgaaaac cggcagcagt
    10441 tgctaacacc cattcaatcg cttgcggttt gatttccgca ctttcaaatt cacgttggcg
    10501 ttcggcagag cgtccgtccg gctcgtacca atagccaaaa tcttcaagtt tacgccgagc
    10561 ttctccagca acgagccaat gggcaatttc gtgcaaggca ctgctgtaat agccgtgagc
    10621 aaataaaatc acattgtaag ggcgttcgct cggcacacca tcttcgtcca taaattcggg
    10681 caagtaaatc ggataattac ccccccgctc taggcgagtg ttatattcat cagaaaagca
    10741 ttgattaaaa atgcgaataa tgtcttcaat tttatgattt tcttgattca tttcttttct
    10801 ttataactta ttcagccaag ctaaaaattg ctcggcatcc aaaaagccag taacacgaga
    10861 ttggctcatt tcattgccgt tttcatcaaa aaacagaatc gttggcaaac ctaatacatt
    10921 aaagtgtttc atcagctcga tattttcagc cgagtttttc gtcatatcga tttgcaacac
    10981 taccatttca ttcagtttgt tctgaacatt ttggtcacta aaggtgtatt tttcaaactc
    11041 tttgcaggcg acacaccagt cggcatagag atcgagcatc acttttttac ctttggaagc
    11101 ggtcaatttt gtctctaaat cggcaagtga ttgaattttt tctaacgcta agtgcggtgt
    11161 agtttgctgt tgagtggttg agccgttcca agctaaatct gcccaaggtt tagcggcaat
    11221 ggctaaagcg agcaataata agactttgat gatctttttc gtgattgaat tagatggaat
    11281 cgcatccacc aaccagatca aaaatgcaac cgcaagggcc gaccataaaa acggttcata
    11341 gtggcttggc agcacacggc ttaataagaa caccggtaag gcaagcataa caaaaccgaa
    11401 tgcagctttc acttttaaca gccattcgcc ggattttggc agaattttgt tgccgaataa
    11461 ggtaatcaaa atcagcggta agcccattcc cagtgcgagt agatagaggg ctaagccacc
    11521 ggtgactaaa tcgccacttt gtgccacata aagcaacgca ccggaaagtg gggcagaagt
    11581 acaaggggaa gcaattaagc ctgcgatcat ccccattaaa aacacgctac ccaacgcacc
    11641 accttgctgt ttttggctca ttgcattgag tttttgttgc cagctatttg gtagctggat
    11701 ttcgtataag ccaaacattg agcaggcaag cagaataaag acgatagata aaccaatcaa
    11761 caccggtggg ctttgtaagg cgatttggaa aggcaagccg atagccgcta caattaagcc
    11821 aagtaaggtg taagtaactg ccattccttg cacataggca atacttaaca gcaatgcttt
    11881 agcggtagaa gggcgttgct tatttccaat tacaatcgca gagagaagtg gtagcatcgg
    11941 cagtacgcaa ggagtgaatg ctaatccaac gcctaataca aaaaaccaaa aaatggaaaa
    12001 acgattgtta gctaaatttt cagccagttg atcttgctct gcttttgcca cattttcagg
    12061 gggcgccata ttgtcggttt ttgcaaaatt ttcgctcttt tcgaccgctt gtgcttccag
    12121 ttgcgccagt tcttcaggtg tagcagagag aatactgtct aatttaagct caacccgttc
    12181 cggtggatag caaaagcctt tggtacaccc ttgatattcc actacgattg aggcgttgtc
    12241 tttaatttct ttaaacggta cctttagccc taagtggttg cgatagattt ccacctgccc
    12301 gaaaaattca tcgttatatt gctcggcagc gggaaaagtg atgtcgccta tctcaatatt
    12361 ctgtggcgtg acacggattt cttttttata aagataataa ttgtctgcaa tatcccaatt
    12421 taactgtaat gtttcatttt cattttggat tgaaaaaaca aaggcttctt ccgattttag
    12481 gaatttgggt tgagagaaaa gatttgcgtg agatgagttt atcatcaata aactcaagac
    12541 gagtgaaaaa aagtatctaa tcatattgaa atcctatttt taattgtctc ggcgaaaatt
    12601 atagcgtaat cagtattcat attccaaata aatta
//
LOCUS       DANLUQ010000065        12516 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.065, whole genome shotgun sequence.
ACCESSION   DANLUQ010000065 DANLUQ010000000
VERSION     DANLUQ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 12516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12516
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.065"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(313..495)
                     /locus_tag="PXB27_001389"
     CDS             complement(313..495)
                     /locus_tag="PXB27_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346170.1"
                     /translation="MYQFGYLRTKIGTDTQRAMLWNCPTYVLIIPFLLNTSVKKQYHA
                     FWQKYHQHNNDVSFPY"
     gene            645..1685
                     /gene="selD"
                     /locus_tag="PXB27_001390"
     CDS             645..1685
                     /gene="selD"
                     /locus_tag="PXB27_001390"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020909986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:690..692,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HDL5346171.1"
                     /translation="MSEPIKLTQFSHGAGUGCKISPKVLGTILQTQLEQFVDPNLLVG
                     NETADDAAVYDLGNGQAIISTTDFFMPIVDAPFDFGRIAAANAISDIYAMGGKPIMAI
                     AILGFPVNKLPAEVAQQIVEGGRFACQQAGIALAGGHSIDSPDPIFGLAVTGIVPTER
                     VKRNASAVAGCELFLTKPLGIGILTTAEKKGLLKPEHQHLASEVMCQINLIGSAFSEL
                     PEVTAMTDVTGFGLLGHLSEVCQGSGLRAEVDFEDIQVLDGTKEYIALGAVPGGTNRN
                     FDSYGHLISAMTDEQKAILCDPQTSGGLLVAVLPQAVEKVQQIANRANVPLFHVGRLL
                     EMEQGKPRIEVI"
     gene            1685..2158
                     /locus_tag="PXB27_001391"
     CDS             1685..2158
                     /locus_tag="PXB27_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL5346172.1"
                     /translation="MFKPNVTMACVVHCKGKFLFVEEIEYGKRTLNQPAGHLEKDETL
                     LEGASRELFEETGIRAEMANLVKIYQWQAPRSQTDFLRFLFSVELEEWLEPNPQDADI
                     TQALWLTLAEFEDYIQQQGQCARSPLVVQSVKDYLSGERYPLSLLNAFEWARNGK"
     gene            2148..3356
                     /gene="ubiH"
                     /locus_tag="PXB27_001392"
                     /gene_synonym="visB"
     CDS             2148..3356
                     /gene="ubiH"
                     /locus_tag="PXB27_001392"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HDL5346173.1"
                     /translation="MENKTTTQFDVVIVGGAVTGSVLALALSSFTHHKISIAIVEKSL
                     PDYEQQGGFDARSIALAEGSLQKMGQIRPLAGSSLGEMISEIGTPIQQIQVADKGHFG
                     KTTLKASEQNVAQLGMVVELAKFGRKLTACLAQQANITLFCPNQVVGIERSQQHCGLS
                     LKNGEKLSCSLLVAADGIQSQIAKQCGVETVKVRDYAQSAIIANVELSESHQNQAFEY
                     FTPQGPFALLPLSGNMMSLVWCVKEPNELIQLSDSEFLARLQQQFGWKLGQFVRVSKR
                     FVYPLILQKAQSHIHHRLAVVGNAAQMLHPVAGQGFNLGLRDLTTLAELVALAFNQGK
                     DIGEFSLLSTFENERTQDQARMINATSGLISLFCCEMLPVQVVRNLGLLTVSHSKIAR
                     DFVANQALGW"
     gene            3619..4851
                     /locus_tag="PXB27_001393"
     CDS             3619..4851
                     /locus_tag="PXB27_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease"
                     /protein_id="HDL5346174.1"
                     /translation="MTTENMSTPKQAFIGLQMLFVAFGALVLVPLITGLDPNVALLTA
                     GVGTLIFQICTKRQVPVFLASSFAFIAPIQFGVQQWGIPVTMGALLCSGFIYMALSAL
                     VKAKGVNILHKYFPPIVIGPVIMVIGLGLAPVAVDMATGKSANIAYNTAIIVSMITLV
                     TTLIVAVFAKGLLKLVPILAGLTVGYILSVFMGLVDFTKVVEAPWFSLPKVSAPEFKL
                     DAILYLLPIALAPAVEHLGDVMAISQVAGKDFMKKPGLHRTLLGDGLATSAAAALGGP
                     PNTTYSEVTGAVMLTKNFNPKIMTWAACWAIAFSFCGKIGAFLATIPTFVMGGIMMLL
                     FGAVAVVGINTLIKAKVDLSIPRNLCIASVVMTFGIGGMLINIGEFSLKGISLCALVA
                     IVLNIVLPKEKVEDSAAH"
     gene            4918..5904
                     /locus_tag="PXB27_001394"
     CDS             4918..5904
                     /locus_tag="PXB27_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDL5346175.1"
                     /translation="MQKLTFSPPLLNTPCPWCSELESLRELYACEYTGAVTTRTSMPQ
                     PYPHDWAKNQYVLFDSNAQKTASVNTQDATSLQTASLNTIGLSPNNLDTTISFVRTIS
                     NELTAPSSKPFIISVFGSPEEVGECYEKIVAFQTEVKMPLAMEINISCPNIPGEISPA
                     YSAEELSHYLHALQTSLKKLAQGDNTLPIGIKIPPFTYQNQYDELIKGLLQAVENGEN
                     LPISFITSTNTLGSSLVLNAGKAVLNSEVGTGVGGMAGTAIHALSLGNVYTIRQMLDQ
                     EPKLKHLQIIGVGGVNDKAGFERMKSVGADFVGLATALGVKGIGVFKEILSE"
     gene            complement(5945..6355)
                     /locus_tag="PXB27_001395"
     CDS             complement(5945..6355)
                     /locus_tag="PXB27_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HDL5346176.1"
                     /translation="MLYSVLTAHILLNEPYRNGAHSTQHEHLVVMFEIDEESGYFYAM
                     DLHQTEQPVVDSLFVFRVSDIEQNSLKEPRRLEICWSEDGYQAFLLINGYPHAVFDFR
                     QFVGYNHTKFPMPELGSMWVHKETTNELVEKWLG"
     gene            complement(6421..7731)
                     /gene="eno"
                     /locus_tag="PXB27_001396"
     CDS             complement(6421..7731)
                     /gene="eno"
                     /locus_tag="PXB27_001396"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDL5346177.1"
                     /translation="MAKIVKVIGREIIDSRGNPTVEAEVHLEGGFVGLAAAPSGASTG
                     SREALELRDGDKGRFLGKGVLKAVSAVNNEIANALVGKDGSAQAEIDQIMIDLDGTEN
                     KSKFGANAILAVSLATAKAAAASKGLPLYAYIAELNGTPGVYSMPLPMMNIINGGEHA
                     DNNVDIQEFMIQPVGASTLKEALRIGAEVFHNLAKVLKAKGLNTAVGDEGGFAPNLAS
                     NADALACIKEAVEKAGYVLGKDVTLAMDCASSEFYDKERNVYDMKGEGKTFTSQEFTH
                     YLEGLCKEYPIVSIEDGQDESDWEGFAYQTKVLGDKVQLVGDDLFVTNTKILKNGIEK
                     GIANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQ
                     IKTGSMSRSDRVAKYNQLIRIEEALERAGTPAPFNGRKEIKGQA"
     gene            complement(7922..9100)
                     /gene="rlmC"
                     /locus_tag="PXB27_001397"
     CDS             complement(7922..9100)
                     /gene="rlmC"
                     /locus_tag="PXB27_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HDL5346178.1"
                     /translation="MSSLLHCSHFERGDCTSCQWIEKPYSTQLAAKEADLKRLILPFI
                     LQNKTEILPLVGSPISQFRNKAKMVVSGSVERPILGILKDQNDAQSGVDLTDCLLYPS
                     SFHRLFPILKDFIARAGLVPYNISKKKGELKYILITESQYNQSFMLRFVVRSEAKRAL
                     VERELPELLEKLPPNSVVSLNIQPQHAALLEGETEIFLTEQKVLEEHFNGIPLFIRPQ
                     GFFQTNPVVASQLYHTAQSWIEHLPIAKLWDLFCGVGGFGLHCANALQAKNSNVELTG
                     IEISASAIASATKSAEKLGLKQVKFASLDSAQFALNEAGSPPDLVIVNPPRRGIGKLL
                     AEFLNQLGSPYLIYSSCNAVSMANDFTFLTNYELHKVQLFDMFAHTAHYETLTFLVKK
                     "
     gene            complement(9093..10130)
                     /gene="nagZ"
                     /locus_tag="PXB27_001398"
     CDS             complement(9093..10130)
                     /gene="nagZ"
                     /locus_tag="PXB27_001398"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HDL5346179.1"
                     /translation="MLLIDIKDKELTQEEVEMLEHPLVSGLILFTRNFYDKAQIQALV
                     KSIRQRVKKPLLITVDQEGGRVQRFREGFTKLPAMQSFNQLATTPEQAMQLAKETGWL
                     MAAEMFALDIDLSFAPVLDLGHECKAIGDRSFGQEPAAILPVAEAFIDGMREIGMATT
                     GKHFPGHGHVLADSHLETPFDDRPKERIFNHDILPFKQLIAKGKLSAIMPAHVVYTQC
                     DSQPASGSSYWLKEVLRKQLNFNGVIFSDDLGMKGAGFMGNFVERSEKAIQAGCDLLL
                     LCNEPEGLVQVLDGLKYQPTQAQKERRLSLMKRKTVTWRELENSSRYQQTQAELTALQ
                     NNWLEWKEANV"
     gene            complement(10256..10987)
                     /locus_tag="PXB27_001399"
     CDS             complement(10256..10987)
                     /locus_tag="PXB27_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDL5346180.1"
                     /translation="MFDWITNPEAWVALFTLTGLEIVLGIDNIIVISILVSRLPIEQR
                     QSARIIGLGLAMGTRILLLLSLAWMMRLVEPLFTIGNNPISGRDLILLIGGLFLIVKS
                     AMELKESISAESHEEKSESIKKASFFMVLIQIAIFDIIFSLDSVITAVAMADDIPVMV
                     IAIIIAVAVMMLAAKTIGDFVDNNPTIKNLALAFLILIGIVLVGEGFDIHIPKAAVYT
                     AMGFSVVVEMLNIKMRRNQAKLEQA"
     gene            complement(11076..11756)
                     /gene="mutH"
                     /locus_tag="PXB27_001400"
     CDS             complement(11076..11756)
                     /gene="mutH"
                     /locus_tag="PXB27_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HDL5346181.1"
                     /translation="MQLSTFSPTEQALLSKAEWLAGFTLGEIAEMLHIPIPADLKRDK
                     GWVGTLIETALGAKAGSKAEQDFAHLGIELKTIPVNQKGLPLETTFVSLAPLTQNNGI
                     IWETSHVKHKLSRVLWIPVEGERKIPLAERHIGQPILWSPTITQESRLQQDWEELMEL
                     IVLGELHKINATLGEVLQLRPKGRNNRSITSAINAKGEIVQSLPLGFYLRKHFTAEIL
                     QNFLHCPL"
     gene            complement(11848..12483)
                     /gene="lexA"
                     /locus_tag="PXB27_001401"
     CDS             complement(11848..12483)
                     /gene="lexA"
                     /locus_tag="PXB27_001401"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HDL5346182.1"
                     /translation="MRKHLTARQQEIFDFVKNHIETTGMPPTRVEIAREIGFKSPNAA
                     EEHLKALARKGYIEMLSGTSRGIRILVEDENQAANDEDEGLPLIGKVAAGTPILAVEH
                     VENHYPVNGAMFNPSADYLLKVNGNSMEKIGILDGDLLAVHKTNTARNGQVVVARVND
                     EVTVKRLEKKGELIYLHPENDELKPIVVNPAQEYIEIEGIAVGVIRSNAWM"
BASE COUNT         3503 a         2544 c         2796 g         3673 t
ORIGIN      
        1 aggaaaaatg tgctgtggca gaacaccgat ggcaacctta cttgatggaa aacggatttg
       61 ggtggaaaag aatttaagct caaattaatc tgacagacac ggtaatttaa aacgggagac
      121 tgtcagatca agtctgatct tctacaacta agccttctca gaataaataa attttcacaa
      181 gcggtgcaac tttgcaaaaa cttcggtatt atgtagcagt tgcacaaaac cccaaattca
      241 ttaaaaccgc ttaaaaatga aatgccctaa actgattttt cagtctaggg catttattat
      301 cacatattat ggttaatagg gaaaactcac atcattgtta tgctggtgat atttttgcca
      361 aaatgcgtga tattgttttt tcaccgatgt gtttaataaa aacgggatta tcaagacata
      421 ggtcgggcaa ttccatagca ttgcacgttg tgtatccgtg ccgattttgg tgcgtaagta
      481 accaaactga tacattagac ccaatccaac cgtcacctat tctaatagca taataactaa
      541 ggtcgcatca ttaaattaaa agctcatttt ttgcaaaaaa tttcccaaat tccaccgctt
      601 gttggcgtac aataggcata attttgattt acgatggagg cagaatgtct gaaccgataa
      661 aacttacgca gttcagccac ggggcgggtt gaggctgtaa aatttcgcct aaggtgttag
      721 ggacaatttt acaaactcaa ttagaacaat ttgtcgatcc gaatttattg gtaggcaatg
      781 aaacggcgga cgatgctgcc gtctatgatt tagggaacgg gcaggccatt atcagcacca
      841 ccgatttctt tatgccgatt gtggacgccc cgtttgattt cgggcgaatt gctgctgcca
      901 atgcgattag cgatatttat gcgatgggtg gcaagccgat tatggcgatt gcgattctgg
      961 gctttccggt gaataaattg ccggctgaag tggctcaaca aattgtcgaa ggtgggcgtt
     1021 ttgcgtgcca gcaagccggt attgccttgg cgggcgggca ttcgattgat tcgcccgatc
     1081 cgattttcgg cttggctgtt accggcattg tgccgaccga acgggtgaaa cgcaatgcct
     1141 ctgcggtggc tgggtgtgag ctgtttttaa ccaagccttt ggggattggc attctaacca
     1201 cggcagagaa aaaaggtttg ttgaagcctg aacatcaaca tctggcaagc gaggtaatgt
     1261 gccaaatcaa cttgattggt tcagcgtttt ccgaactgcc tgaagtcacg gcaatgactg
     1321 atgtaaccgg ttttggctta ctcgggcatt taagcgaggt gtgtcaaggc tcgggcttac
     1381 gggcggaggt tgattttgag gatattcaag tccttgatgg cactaaggag tatattgcat
     1441 tgggagcagt tccgggtggg acaaatcgca atttcgacag ctatgggcat ttgatttcgg
     1501 caatgacgga cgagcaaaaa gcgattttgt gcgatccgca aacctctggc ggtttattag
     1561 tggcggtgct gccacaagcg gtcgaaaaag tgcaacaaat tgcaaatcgg gcaaatgtgc
     1621 cgttattcca cgttggcaga ttgctggaaa tggaacaggg caaaccacgg attgaggtaa
     1681 tttgatgttt aaaccaaatg taacaatggc ttgcgtggtg cattgtaagg gaaagtttct
     1741 gtttgtggaa gaaattgaat atggcaaacg tacccttaac cagccggcag ggcatttaga
     1801 aaaagatgaa accttgctgg agggggcaag ccgtgagctg tttgaggaaa ccggtattcg
     1861 ggcagaaatg gcaaatttgg tgaaaattta tcaatggcaa gcccctcgtt cacaaaccga
     1921 tttcttgcgt tttctgtttt cggtggaact ggaagagtgg ctggagccga atccgcaaga
     1981 tgcggatatt acccaagccc tttggcttac tttagcagaa tttgaagact acattcagca
     2041 acaagggcaa tgtgccagaa gcccgttggt ggttcaatcg gtcaaagatt atttaagcgg
     2101 tgagcgttat ccattaagtt tgttgaatgc gtttgaatgg gcgagaaatg gaaaataaaa
     2161 caacaaccca atttgatgtg gtgattgtgg gcggggcagt gaccggctcg gtgctggcat
     2221 tggcgttaag tagttttact catcacaaaa tctcgattgc gatagtggaa aaatcgctgc
     2281 cggactatga acagcaaggc ggttttgatg ctcgcagtat tgccttagcc gaaggcagtt
     2341 tgcaaaaaat ggggcaaatc agaccgcttg caggctcaag tttaggcgag atgattagcg
     2401 aaatcggcac gccgattcag caaattcaag tagcggataa agggcatttt ggtaaaacga
     2461 ccttgaaagc aagcgagcag aatgtggctc aactgggtat ggtggtggaa cttgcaaaat
     2521 tcggcagaaa attgaccgct tgtcttgcac agcaagctaa tattacgctt ttttgcccga
     2581 atcaagtggt tggcattgag cgttcccaac aacattgtgg attgagcttg aaaaatggtg
     2641 agaaactaag ctgttccttg ctggtggcgg cagatggtat tcaatctcag attgcaaaac
     2701 agtgcggtgt ggaaaccgta aaagtaaggg attatgcaca atcggcaatt atcgccaatg
     2761 tggaactgag tgaatctcac caaaatcaag cctttgaata ttttacgcct caaggccctt
     2821 ttgctttatt gcctttgagc ggaaatatga tgtcgttggt gtggtgtgtg aaagagccga
     2881 acgagttaat ccaactttca gacagtgaat ttttagcccg tttgcaacaa caatttggtt
     2941 ggaagttagg gcaatttgtt cgagtgagca agcggtttgt ttatccgctc attttgcaaa
     3001 aagcccagtc gcatattcac caccgcttag cagttgtcgg caatgccgca caaatgcttc
     3061 acccggttgc tgggcaaggt ttcaacttag gcttgcgtga tttgactacc ttagcggaat
     3121 tagtggcttt ggcgtttaat caagggaaag atataggcga attttcgctc ttatcaactt
     3181 ttgagaatga gagaacgcaa gatcaagcca gaatgataaa tgctacaagc ggtctgattt
     3241 cgcttttttg ttgcgaaatg ttaccagttc aggttgtgag gaatttaggg ttattaacgg
     3301 tttcccattc aaaaatagcc agagattttg ttgctaatca ggcattaggt tggtaaaaaa
     3361 cacaaaagca gcaattttct atttgacact gtttttaaaa aggcgtatta tccaacccgt
     3421 ttaaaaatct caccaaagcc ttgaatgcaa attcaaggct ttttcctttt tagtgaaagc
     3481 ctatgaattt tgtttgccgt aaaatcggca aaaaaatttt gcctaaaatt cgcaatcgtt
     3541 tgcgaaatag gtttatagcg ttgttttcat ctgggtaact gcaatggtta catcgcaata
     3601 catcaaagag agtaaaaaat gaccacagaa aatatgagta cgccaaagca ggcgtttatc
     3661 gggctgcaaa tgctgtttgt tgcatttggg gcgttagtat tagttccgct gattaccggt
     3721 ttagatccta atgttgccct tcttaccgcc ggtgtcggta cgttaatctt ccaaatttgt
     3781 actaaacgtc aagttcctgt tttcctcgca tcgagtttcg cttttatcgc tccaatccaa
     3841 ttcggtgttc aacaatgggg aattcccgtc acaatgggag cattgctctg ttccggtttt
     3901 atttatatgg cattatctgc tttagtgaaa gcgaaagggg tgaatattct gcataaatac
     3961 ttccctccaa tcgtgattgg accggtaatt atggtgattg gtttaggtct tgctcctgtt
     4021 gcggtggata tggcaacggg taaatcggcg aatattgcct ataacaccgc tattattgtt
     4081 tcaatgataa cgctggtaac tacgctgatt gtggcggtgt ttgcgaaagg gctattaaag
     4141 ctcgtgccaa tattagcagg tttaacggtg ggatatatct tgtctgtttt tatgggattg
     4201 gtggatttta ctaaagtggt tgaagctcct tggtttagtc taccgaaagt ttccgcaccg
     4261 gaattcaaat tagatgcaat cttgtattta ttacctatcg cacttgctcc ggcagtcgaa
     4321 cacttaggcg atgtaatggc aatcagccaa gttgccggta aagattttat gaaaaaaccg
     4381 gggttacacc gcacgctttt aggtgatggt ttagcaactt cagcagctgc ggcattaggt
     4441 ggcccaccaa atacgactta ctctgaagta accggtgcgg taatgctaac caaaaacttc
     4501 aatccgaaaa ttatgacttg ggctgcgtgc tgggctatcg ctttctcatt ctgcggaaaa
     4561 atcggggcat tccttgccac tatccctact tttgtgatgg gcggtattat gatgttgctg
     4621 ttcggtgcag ttgcggttgt ggggatcaat actctaatta aagctaaagt tgatttaagc
     4681 attccacgta atctttgtat tgcttctgta gtaatgacat tcggtattgg cggtatgtta
     4741 attaacatcg gcgaattctc attaaaaggg atcagcttgt gtgcattagt cgctattgta
     4801 cttaacattg tattaccaaa agaaaaagta gaagattctg ctgctcatta atcaaaggac
     4861 gctgtggcgt cctttttgct aataaccctc tatttgtaaa taaaaaggaa taacactatg
     4921 caaaaactca cattttcacc accactttta aatacgcctt gcccttggtg ttcggagtta
     4981 gagagcttgc gtgaacttta tgcgtgtgaa tataccggtg cggtaacaac ccgtacttca
     5041 atgcctcaac catatccaca cgattgggcg aagaaccaat atgtactctt tgattccaac
     5101 gcacagaaaa cagcaagtgt gaatactcaa gatgcaacta gcttacaaac agcaagcctt
     5161 aacaccatcg gtttaagccc aaataatctg gataccacca tcagctttgt acgcaccatt
     5221 tcaaatgagc tgacagcgcc atcaagcaaa ccatttatta tttcggtgtt tggttcaccc
     5281 gaagaggtgg gggaatgtta tgaaaaaatt gttgctttcc aaactgaagt gaaaatgcct
     5341 ttggcaatgg agattaatat ctcttgcccg aatattccgg gtgaaatttc gcctgcttat
     5401 tccgcagaag aattatcaca ttacttgcac gcactacaaa cttcgctaaa aaaactggct
     5461 caaggtgata atactttacc aatcgggatt aaaattccac cttttactta ccaaaatcaa
     5521 tatgatgaat taatcaaagg cttattacaa gcggtcgaaa atggcgaaaa tttaccaatt
     5581 tcctttatta cttcaaccaa tacgcttggc tcatcgcttg tcttaaatgc tggcaaagcg
     5641 gtattaaatt cagaagtggg aacgggcgta ggcggaatgg caggaacggc aatccatgca
     5701 ctttctttgg ggaatgttta cacgattcgc caaatgttag atcaagagcc taagctcaaa
     5761 catcttcaaa ttattggtgt gggcggcgtg aatgataaag ccggctttga gcgaatgaaa
     5821 tcggttgggg cagactttgt ggggttagct accgcattag gggtgaaagg aatcggcgtt
     5881 tttaaagaga ttctgtctga ataatgaaga ggagggcgtg cattgcacgc ccgaataaat
     5941 gaaattagcc taaccatttc tccactaatt cattagtggt ttctttatgt acccacatac
     6001 tgccaagttc cggcattggg aatttggtgt ggttgtagcc gacaaattgg cggaaatcaa
     6061 acacggcgtg cgggtagccg ttgatcagta aaaaggcttg atagccgtct tcactccagc
     6121 aaatttctaa acggcgaggt tcttttaatg aattttgctc aatatcgctg acccgaaaca
     6181 caaataaact atccactacc ggctgttcgg tttggtgtaa gtccattgca taaaagtagc
     6241 cgctttcttc atcaatttca aacatcacta ctaaatgttc gtgctgggtt gaatgagcgc
     6301 cattacggta aggttcgttc agtagaatat gagcagttaa aacagagtag agcattaaat
     6361 tatccttata agtaaaatgc caccgatttc tcagtggcat ttaaatattt aatcaggcaa
     6421 ttacgcttga cctttaatct ctttacggcc gttgaacgga gccggtgtac cagcacgctc
     6481 taacgcttct tcgatacgga ttaactggtt gtatttcgct acacggtctg aacggctcat
     6541 tgaaccggtt ttgatttgac ctgctgctgt acctactgct aaatctgcaa tggttgcatc
     6601 ttcagtttcg cctgaacggt gagagataac tgcggtgtaa cctgcatctt tcgccatttt
     6661 gattgcagct aaagtttcag ttaaagaacc gatttggttg aatttgatta agattgagtt
     6721 tgcgatacct ttctcaatac cgtttttcaa gattttggta ttggttacga ataagtcatc
     6781 accaactaat tgtactttgt cgcctaatac tttagtttgg tatgcgaaac cttcccagtc
     6841 agattcgtct tgaccatctt cgatagacac gattgggtac tctttgcata agccttctaa
     6901 gtagtgagtg aactcttgtg aagtaaaggt tttgccttcg cctttcatat cgtacacatt
     6961 gcgttctttg tcgtagaatt cagatgatgc acagtccatc gctaaagtta cgtctttacc
     7021 taacacatag cctgcttttt ctaccgcttc tttaatgcac gctaatgcgt ctgcgtttga
     7081 agccaagttt ggtgcgaaac caccttcgtc acctacagca gtatttaagc ctttcgcttt
     7141 taatactttt gcaagattgt ggaatacttc agcaccgata cgaagagctt cttttaatgt
     7201 agaagcacct accggttgaa tcataaattc ttgaatgtcc acgttgttgt cagcgtgttc
     7261 accaccattg atgatgttca tcattggtaa tggcattgag taaacacccg gtgtgccgtt
     7321 taattcagca atgtatgcgt aaagcggtaa gccttttgaa gcggctgctg cttttgctgt
     7381 tgctaaagaa accgctaaga tagcgtttgc accgaatttt gatttgtttt ctgtgccatc
     7441 taaatcgatc ataatttggt cgatttcagc ttgtgcagaa ccgtctttgc ctactaatgc
     7501 gtttgcaatt tcgttattta cggcagaaac cgcttttaat acacctttac ctaagaaacg
     7561 acctttgtcg ccatcacgta attctaatgc ttcacgtgaa ccggtagaag cacctgatgg
     7621 agcggctgct aagcctacaa aaccaccttc taagtgaact tctgcttcta cagttgggtt
     7681 accacgagag tcgatgattt cacgaccgat tactttaaca attttagcca ttgttgatgt
     7741 cctctaatat acgagttaat agaaaaaaga ataggtatat agtaaagcga ttttctgctt
     7801 ttgtcttgga aaaactgatt gagtggcaga aaatttgcca ctaatttgat ctaagactat
     7861 attcgcctga gattatagca acctttgcaa aaaacggcaa aaatttgccc gcttgtaatg
     7921 cttatttttt gactaaaaaa gtcaaggttt cataatgtgc tgtgtgagca aacatatcaa
     7981 acagctgcac tttgtgtagt tcgtaatttg tcaaaaatgt aaaatcgttc gccattgaga
     8041 cggcattaca actggagtaa atcaaataag gtgagcctaa ttgatttaaa aattctgcta
     8101 atagtttgcc gataccacgt cgaggtgggt tcacaatcac taaatcgggt ggagaaccag
     8161 cttcatttaa agcaaattgt gcagaatcta atgaggcaaa ttttacctgc tttaggccga
     8221 gtttttcagc cgatttggtc gcacttgcaa tagctgaggc tgaaatctca atgcctgtca
     8281 gctccacgtt cgagttcttc gcttgcaagg cgttggcaca atgtaagccg aaaccaccga
     8341 caccgcagaa caagtcccac aatttagcaa taggtaaatg ctcaatccag ctttgtgctg
     8401 tgtggtaaag ctggcttgct accacagggt tagtttggaa aaagccctga gggcgaataa
     8461 atagcggtat gccgttaaaa tgttcttcca gcactttttg ttcggtgagg aaaatctcag
     8521 tttcgccttc taaaagagca gcgtgttggg gctgaatatt cagactaact actgagttgg
     8581 gtggcagttt ttccaacagt tcgggcaatt cccgttcaac caatgcacgc tttgcttcac
     8641 tacgaaccac aaaacggagc ataaatgatt gattatattg gctttcggta atcagaatat
     8701 attttagctc accttttttc ttactgatgt tatacggcac taaaccagct cgagcgataa
     8761 aatctttcaa aatcggaaaa agtctatgaa atgaggacgg gtagagcaga cagtcggtta
     8821 aatccacccc gctttgagca tcattttgat cttttaaaat gcctaaaatc gggcgttcta
     8881 cactgccgga gaccaccatt ttcgctttgt tacgaaattg cgaaattggc gagccgacaa
     8941 gcggtaagat ttccgtttta ttttgcaaaa taaacggcaa aatcaaccgc ttgagatcgg
     9001 cctctttggc ggctaattgc gtgctgtatg gcttttcaat ccactggcac gaagtacagt
     9061 cgccacgctc aaaatgcgaa caatgcaaca agctagacat tcgcttcctt ccattccagc
     9121 cagttgtttt gtaaggccgt taattcagcc tgagtttgtt ggtagcgaga gctgttttcc
     9181 agctctcgcc aagtcacggt tttgcgtttc attaaagaaa ggcgacgttc tttttgtgct
     9241 tgagtcggct gatatttcag cccgtctaac acttgcacca ggccttcagg ctcgttacaa
     9301 aggagcaata aatcacagcc ggcttggatt gctttttcag aacgctcgac aaaattgccc
     9361 ataaagcccg cacctttcat tcccaaatcg tcagagaata tcacaccgtt aaagttcagc
     9421 tgtttacgca atacctcttt aagccaatag cttgaaccgc ttgccggctg gctatcacat
     9481 tgggtgtaaa cgacgtgggc aggcataatg gcagagagtt ttcctttggc aataagctgt
     9541 ttgaacggca gaatatcgtg attaaaaatc cgctctttcg ggcgatcatc aaacggggtt
     9601 tctaaatgcg aatcggctaa aacgtgtcca tgaccgggga agtgtttgcc ggtggtcgcc
     9661 ataccgattt cacgcattcc gtcaataaag gcttcggcaa caggcaaaat cgctgctggc
     9721 tcttgcccga atgagcgatc gccaatcgct ttgcattcgt gtcctaaatc cagtaccgga
     9781 gcgaaactga ggtctatatc gagggcaaac atttctgctg ccatcaacca gccggtttct
     9841 ttcgctaatt gcattgcttg ctctggcgtt gtggcaagtt ggttaaaaga ttgcattgcc
     9901 ggcagtttag taaagccttc acgaaagcgt tgcacccgac cgccttcttg gtcgacagta
     9961 ataagcaacg gttttttcac ccgctggcga atggatttca ctaatgcctg aatttgtgct
    10021 ttgtcgtaga aattacgggt gaataaaatt agcccggaaa ctaaagggtg ttccagcatt
    10081 tcgacttctt cttgagtcag ttctttatct ttaatatcaa tcagtaacat aattttcgtt
    10141 gagctatttg caaaaaaatg agcagatatg accgcttgtt gtgcctattc tacattaaaa
    10201 agcccttgat gttgatcaag ggcgaatgat taagggcata aatgagacag agcaattatg
    10261 cttgttctaa tttagcttgg ttacgacgca tttttatgtt gagcatttca accacaaccg
    10321 agaagcccat tgccgtataa accgctgctt ttggaatatg aatatcaaaa ccttcgccga
    10381 ctagtacaat accgattaaa atcaagaacg ctaaagcgag atttttaatg gttgggttgt
    10441 tatctacaaa gtccccaatg gttttcgctg ctaacatcat taccgcaact gcaatgataa
    10501 tcgcaataac cattaccgga atatcatctg ccattgccac agcagtaatg actgaatcaa
    10561 gggagaaaat aatatcaaaa attgcaattt gaattaacac cataaaaaag ctggcttttt
    10621 taatggattc gcttttttct tcgtgagatt cggcagaaat ggattctttc agctccattg
    10681 cacttttaac aatcaggaat aatccaccga tgagtagaat gagatctcta cctgaaattg
    10741 gattgttgcc aatagtgaaa agaggctcga ccaaacgcat catccaagca agcgagagaa
    10801 gcaataaaat acgggttccc attgccagcc ccaagccgat aattcgggca gattggcgtt
    10861 gttcaatagg taaacgggaa acgagaatac tgatgacgat gatgttatca atgcctaata
    10921 cgatttcaag cccggtaagt gtaaagagcg caacccaagc ttcaggattt gtgatccagt
    10981 caaacataaa aatgtccttt taaaaataag ggattaagtg atattttcta tcaaatagaa
    11041 ttaaagccga catcttatag agtgtgagga caaatttaaa gggggcaatg caaaaagttt
    11101 tgtaaaattt cggcggtaaa atgtttacgc aaatagaaac caagagggag agattgaaca
    11161 atctcccctt tagcgttgat agcagaggtg atagagcggt tatttctacc tttgggtctg
    11221 agttgtaaca cttcgcctaa ggttgcatta attttatgca gttcacctaa cacaatcaac
    11281 tccattaatt cctcccaatc ttgctgtagg cgggattctt gtgtgatagt cggagaccat
    11341 aaaatcggct gaccgatatg gcgttcggca aggggtatct ttcgttcccc ttcaaccgga
    11401 atccaaagca cacgggaaag cttatgttta acgtgggagg tttcccaaat aataccgttg
    11461 ttttgggtta aaggagcaag gctgacgaat gtggtttcca gcggtaatcc tttctgattg
    11521 accggaatgg ttttcagctc aatgcctaaa tgtgcaaaat cctgttcggc tttactgccg
    11581 gctttcgcac ctaaagcggt ttcaatcaga gtaccgaccc agcctttatc tcgtttcaga
    11641 tctgctggta tcggaatatg aagcatctcg gcaatttcac ctaaagtgaa ccctgccagc
    11701 cattccgctt tgcttaataa tgcctgctcg gttggagaga atgtagataa ttgcattgca
    11761 cttagttaat tagactaaac tggcaagcgg tcaaatttag gcagaaattt gcaaaaaatt
    11821 actaaaaaat aaccgcttgt gaggaaatta catccaagca ttactgcgaa taacgcctac
    11881 tgcaatgcct tcaatttcaa tatattcttg agcagggttt actacaatcg gttttaattc
    11941 atcgttttca gggtgtaagt aaattaattc acctttcttc tctaagcgtt taacagtcac
    12001 ttcatcattt acccgagcaa ccaccacttg cccgttacga gcggtattgg ttttatgaac
    12061 ggctaatagg tcaccgtcta aaataccgat tttttccatt gaattgccat ttactttgag
    12121 taaataatca gcacttggat taaacatcgc accattcacc gggtaatggt tttcaacgtg
    12181 ttcaaccgct aaaataggtg tgccagcagc tactttgcca atcaacggta agccttcatc
    12241 ttcatcgttg gcagcttggt tttcgtcttc gactaaaatg cgaattccac gagatgtacc
    12301 tgataacatt tcaatatagc ctttacgagc aagggctttt aagtgttctt ctgccgcatt
    12361 aggtgattta aagccgattt cacgggctat ttctactcgg gttggtggca taccggtggt
    12421 ttcaatatgg tttttgacga aatcaaaaat ttcttgctga cgggcagtta gatgtttacg
    12481 cattttaagt taatcctcac tgtctttata tacaga
//
LOCUS       DANLUQ010000066        11937 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.066, whole genome shotgun sequence.
ACCESSION   DANLUQ010000066 DANLUQ010000000
VERSION     DANLUQ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11937
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.066"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            152..370
                     /locus_tag="PXB27_001402"
     CDS             152..370
                     /locus_tag="PXB27_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346183.1"
                     /translation="MYQYYLAQVGNEQQKLIRGMPDNLLSFSVYEPEQEDWDNKFGPF
                     WADKILISGFDAYEEISEKEAIRFIKVH"
     gene            371..784
                     /locus_tag="PXB27_001403"
     CDS             371..784
                     /locus_tag="PXB27_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL5346184.1"
                     /translation="MCQAEQFARQLHYGQTDKAGKPYIEHLAFVAENISTKNEETEAV
                     AWLHDSVEDTQCSINDIRHIFGDVIADAVEAITKKQGESYFCYLDRVKSNPIAKVVKL
                     ADLTHNLCLERLPKITEKDLKRAEKYKSALEYLQA"
     gene            902..1636
                     /locus_tag="PXB27_001404"
     CDS             902..1636
                     /locus_tag="PXB27_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346185.1"
                     /translation="MKLKLDENGHVVVENGMPVYIHDDGKEIPFDALKATQKITQLNA
                     EAKQHREAKEKAEADLKAFSGIDDPKAALEALKTVANLDAKKLIDAGDAEKVKAEIIK
                     GYDEKLAVATAQAEKLQQQLHAELIGGSFARSKYAQEHLNIPSDVVQAFFGKHFSISD
                     DGKVVAKFADGGEIFSRTRPGEKADFEEALEALVSAYPNKDAILKPSGSSGAGAGGGS
                     RSSSMPKTLSDCKTDEERIAYLQANS"
     gene            1702..2649
                     /locus_tag="PXB27_001405"
     CDS             1702..2649
                     /locus_tag="PXB27_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346186.1"
                     /translation="MAFDLQVFNKQTQLALTETVDQAIEKFNAASGGAIILQNKPAEG
                     DFDIRASFKGIAGLVRRRNVYGTGTVDAKRLEQLLDVAVKVAAGTAPIEYQPSQYKWI
                     LLNPELAAIEIGTQLAKARVADMLNTAIAGTVAAISGNTAMTLDEKTAAPTFRILNKG
                     AAKMGDRAGSLKAWVLHSTTLHGLYDNALTNAENLFKYENVNVIRDPFGRVFVVTDSP
                     ALMASDGTFNTLGLVENAVVVSGNNDFDSVLVPKTGGENLSAVYQAEWTYNLGILGYK
                     WDMTAGGKSPNDTALGTPTNWDKSVTSNKDTAGVLVKTK"
     gene            2709..3059
                     /locus_tag="PXB27_001406"
     CDS             2709..3059
                     /locus_tag="PXB27_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346187.1"
                     /translation="MKRILYFTTDFSAKNIAFAKAHGVLMRNLNAYHPSDTLENADAV
                     CGNVPQAYQHLPLFELPESNQKSGQDLKTLKTEEIKEKLTELGIAFEANAKKETLLTL
                     LTQAVGRDGTNDAK"
     gene            3049..3486
                     /locus_tag="PXB27_001407"
     CDS             3049..3486
                     /locus_tag="PXB27_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346188.1"
                     /translation="MLNSYMTVEQANAYHDIRMSREAWNALSDQEKQQRLVSASDFLD
                     FNYRFSGEKADLTQEREFPRKGFEAIGIPKAVQFAVCELALQDNLTANQEAQMSSVRV
                     GPVSVNYDNSSPQNSVNRFEYVRQLLSTWLDRSSFGTVKLERS"
     gene            3486..3857
                     /locus_tag="PXB27_001408"
     CDS             3486..3857
                     /locus_tag="PXB27_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346189.1"
                     /translation="MADFYGNLGKISHSLIKQFGQPCSVTINEQGRYNPATGERKKGS
                     STVHAGYCLFDNLAFDFRNQQSVSVQQGDVMIYLTADCKPVLNAVIEANGEKWSVINV
                     QPIRPAATTMIYQVQGRKADG"
     gene            3850..4263
                     /locus_tag="PXB27_001409"
     CDS             3850..4263
                     /locus_tag="PXB27_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDL5346190.1"
                     /translation="MGSFHAQIARFVEQTQQKADRALRVIALQTLSGVQKKSPVDTGQ
                     LRRSWTVALNGFPSSYNGSQTVLNSAKFGDMIVIATNKPYAPMLEYGLYPNPPKKPTG
                     KTKNGYSIQAPNGMVRITVQEMQAFIRNNPQLGVS"
     gene            4263..4655
                     /locus_tag="PXB27_001410"
     CDS             4263..4655
                     /locus_tag="PXB27_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346191.1"
                     /translation="MRPIIRSVLEQHLAELNPFNTVWEGIIDSPKLPYQTVSMTTSTN
                     ETATIGNTPKAKQSGFLQVTLYYPLDQGTKAIEERADEIFRHFFGQVWIEENVQILVQ
                     QPPLVASLYRLDNSIALPVTIYFTAYEL"
     gene            4665..5687
                     /locus_tag="PXB27_001411"
     CDS             4665..5687
                     /locus_tag="PXB27_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346192.1"
                     /translation="MSILGNSQGTKRTVVFAKESSFGTKAAPTGAKIMPRVETSLNTN
                     FESYQSEEIRDDLQRSASVIGFEKVEGDIKGELAAGQWAAFFAAALRGSFTAQAKKPI
                     ITKTTDGTGEKNGKILIVPKTSHTSDSFTLEDVFKDINVSRQYLGCRVSKLSLDVQPN
                     GIASITVSFLGQRGEESTTAYFTNPTDLTQSGKLAGVKGSLQVNKRPVGLVTAFKLDI
                     DLGASSEPVLGAKYAPDVFIGTVAVSGSFTMYFQDKTMIDAVRNGTDLSLALRMDAEE
                     RTNTDYLTIILPGVKVTSNELDDGAKNIMQTLNFDAFPAVWDSASQIDNVLKVATTMI
                     VQDSLA"
     gene            5777..6178
                     /locus_tag="PXB27_001412"
     CDS             5777..6178
                     /locus_tag="PXB27_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346193.1"
                     /translation="MNLNALSKEKQLAEHRFELVHPVSGEPLGVFMSVVSAKSDKAQR
                     FAEKQLRQEQMRELENAKSRKPKFKGLGDMIAESRAMAIDRLTGWENVEWNEKPLEFS
                     DQNAELLLTECDWIVEQIFEHSNDLGKFLKS"
     gene            6193..6519
                     /locus_tag="PXB27_001413"
     CDS             6193..6519
                     /locus_tag="PXB27_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346194.1"
                     /translation="MYAEKTFKLDKKLKGSESTLREHLQAIEMQTGITPEELHNPEPS
                     PAVAYLLGWFFELAMSRQSGMSLNPISYTEIDAWSRLFARHLEQWEISVIKQLDMIYL
                     NVQQTE"
     gene            6521..6844
                     /locus_tag="PXB27_001414"
     CDS             6521..6844
                     /locus_tag="PXB27_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005714385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5346195.1"
                     /translation="MDTFNFQPNWGLNVQRKPEVMKIAFGDGYEQVAPKGLNHNLRTY
                     SAVFTGTEARIKQIEAFFERQGGYKAFLWTPYGGKEGKFRCEEWEVSISSGKWTLSAT
                     IREVL"
     gene            6931..7623
                     /locus_tag="PXB27_001415"
     CDS             6931..7623
                     /locus_tag="PXB27_001415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346196.1"
                     /translation="MKKIFVSALLLTCSLSALGNSSYELIIDSMQDLEKHKPEILSIP
                     EADRNTFIRYMMRKELRIKLNQGRLADGLTVREAIQNQQAFEKDRNSKMQQEKQKKET
                     EKAEKEKAVTDFNQKFELSITGARKAKNALNQDILLVSLSLKNNSDKPIIAVQPDIQF
                     VLGEDKQTISSMRPKKFENGIPPNGSEIFEFGFDLDDMFALKAEKNIEKLRAVFQTAE
                     VLFADGTTEKLQ"
     gene            7687..8511
                     /locus_tag="PXB27_001416"
                     /pseudo
     CDS             7687..8511
                     /locus_tag="PXB27_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691083.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
     gene            10555..10713
                     /locus_tag="PXB27_001417"
                     /pseudo
     CDS             10555..10713
                     /locus_tag="PXB27_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251214.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
     gene            10721..11437
                     /locus_tag="PXB27_001418"
     CDS             10721..11437
                     /locus_tag="PXB27_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDL5346197.1"
                     /translation="MPISISNQMKLDLAKLEQNAMLDLYEVDLRNLKDKSGNAGRVYR
                     FYSGLNELKTSIVWQGRTYDPYPIEASGFERNSSGPSNRPTLTLSNLFGLITSIANQF
                     DECIGAIVRRHQVYAQYLDAVNFAGGNAKADPNQEIISHFVIEQLTSLTRETATFTLA
                     LPIETDNAKIPSRIIMADTCTWIYRSAECGYTGKQYFDEKDKSTSDPKADKCSHCLNG
                     CELRGNQRNFGGFVSVNKLG"
     gene            11441..>11937
                     /locus_tag="PXB27_001419"
     CDS             11441..>11937
                     /locus_tag="PXB27_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5346198.1"
                     /translation="MELEQQIIDYALQHEPHEMCGFVVFDGNKNRFIPCENQAEDKAN
                     YFEISDLDYIKAEEKGELVAVVHSHPEPNGKPVLSTLDRKMQVQTGLDWWLVHNRQIH
                     KFRNVPHLIGREFKHGTMDCYTLYRDAYMLAGYEMDEFERQDDWWHTGQNLYLDNIQG
                     QGFERV"
BASE COUNT         3752 a         2352 c         2810 g         3023 t
ORIGIN      
        1 tgaaaaacgg taaattgcac gagctctatg aaaaattcag agatctgaat atacctcttg
       61 caaaaaaatc agaaatttta cttgaaatgc agaagttaag tgctaatcta aaagagccaa
      121 agaacattga tttaatcaag gagaaaaaat aatgtatcag tattatttag ctcaagttgg
      181 caatgaacag caaaaactta ttcgtggtat gcctgacaat ctacttagtt tctcggttta
      241 tgagcctgaa caagaagatt gggataataa gttcggccca ttttgggcag acaagatctt
      301 gataagcgga tttgacgctt acgaggaaat ttcagaaaag gaagcaatcc gatttatcaa
      361 ggtacattaa atgtgtcaag ctgaacaatt cgctcgccaa ctgcattatg gacaaacaga
      421 taaagctgga aagccataca ttgagcatct tgcttttgtt gcagaaaaca ttagcacaaa
      481 aaatgaagag acagaagcag tagcttggtt gcacgacagt gtagaagata ctcagtgttc
      541 aattaatgat attcgtcata tatttggcga tgttattgct gatgcagttg aggctattac
      601 taaaaagcaa ggcgaaagtt atttttgcta cttggataga gtaaaatcaa atcctattgc
      661 taaggtggtt aagttagctg atttgactca taatctttgc cttgagcgat tgcccaagat
      721 cactgaaaag gatcttaaac gagcagaaaa atacaaatca gcattggaat atctacaagc
      781 ctagcctaaa acgctaggct tttttatcgc aattttttac cccaaactga ccgtttgtga
      841 gccatcacag gcggtttttt attatcgggc ggaagcccac cagcaatgag aggaagctca
      901 aatgaaatta aaattagacg aaaacggaca tgtagtagtt gaaaatggta tgcctgtgta
      961 tattcacgat gacggaaagg aaattccgtt cgatgcacta aaggcgacac agaaaatcac
     1021 acaactgaac gctgaggcaa agcaacatcg agaagctaaa gagaaagcgg aagccgactt
     1081 aaaagcattc tcagggattg acgatccgaa agctgcactc gaggcattaa agacggttgc
     1141 caatttagat gctaaaaagc tgattgatgc cggtgatgcg gaaaaagtca aagcggaaat
     1201 catcaaaggt tacgatgaga aattagctgt ggcaaccgct caagccgaaa aactgcaaca
     1261 gcagttacac gctgaattaa tcggtggttc gtttgctcgg tctaaatacg ctcaagaaca
     1321 cttgaatatt ccgtctgatg tcgtacaggc attcttcggt aagcatttta gtatttcgga
     1381 tgacggcaaa gtggtagcta aatttgctga cgggggcgaa atctttagtc gcacccgccc
     1441 gggcgaaaaa gcagattttg aagaggcatt agaagcttta gtaagtgctt atccaaataa
     1501 agacgctatt ttaaaaccaa gtggttcaag tggggcagga gcaggtggcg gatcacgcag
     1561 ttcatctatg ccgaaaacat tatcggactg caaaacagat gaagagcgta tcgcttatct
     1621 tcaagcaaat tcgtaaggtg caagaggttg cacctttttt attttcggtg caatcgcacc
     1681 actttttaga ggaaaatcaa aatggcattt gacctacagg tttttaacaa acaaacgcaa
     1741 ttagccttga ccgaaactgt cgatcaggct attgaaaaat tcaatgcagc atcaggcggt
     1801 gcaatcattt tacagaacaa gccagctgaa ggtgattttg atatccgtgc tagctttaaa
     1861 ggtattgccg gtttagttcg ccgccgtaat gtgtacggta caggtacggt agatgcaaaa
     1921 cgtttagagc aattactgga cgtagcagtt aaagtggcgg caggtacagc accgattgaa
     1981 taccagccga gtcaatataa atggatttta ttaaatcctg aattggcggc gattgaaatc
     2041 ggcacacaac ttgccaaagc ccgtgttgct gatatgctca atacggctat tgccggcacg
     2101 gttgctgcaa ttagtggcaa tacagcaatg acacttgatg aaaagaccgc cgctccgaca
     2161 ttccgcattt taaacaaagg tgcagcgaaa atgggtgatc gtgccggttc gcttaaagcg
     2221 tgggtgttgc actccacaac cttacacggc ttatacgata atgccttaac caatgcggaa
     2281 aacttattca agtacgaaaa cgtgaacgta attcgtgacc cgtttgggcg tgtatttgtc
     2341 gtcacagaca gcccggcttt aatggcttca gacggtacat ttaacacctt aggcttggtt
     2401 gaaaatgcgg tggtagtttc cggcaacaat gactttgaca gcgtgttagt gccgaaaacc
     2461 ggcggagaaa acctttcagc agtttaccaa gctgaatgga cctataactt aggaatccta
     2521 ggttataaat gggatatgac cgctggtggt aaatcaccga acgataccgc cctaggcaca
     2581 ccgacgaact gggataaatc ggttacctcc aacaaagaca ccgccggtgt attggtaaaa
     2641 acgaagtaat ttttaaccgc ttgtagataa ggtttacaag cggttgtttt tttatctgga
     2701 gtttgcaaat gaaacgaata ctttatttta caacggattt ttcggcaaaa aacattgcct
     2761 ttgccaaagc tcacggtgta ttaatgcgga atctgaacgc ttaccaccca tcagacacct
     2821 tagaaaatgc agatgcagtg tgcggtaacg tacctcaagc ttatcaacat ttaccgttgt
     2881 ttgaattgcc tgagtctaac caaaaatcag gtcaagattt gaaaacctta aaaaccgaag
     2941 aaattaagga aaaactgact gaattaggta ttgcttttga ggcgaatgcc aaaaaagaaa
     3001 cattactaac cttactcact caggcggtgg gacgtgacgg aacaaacgat gctaaatagt
     3061 tatatgacgg ttgagcaagc caacgcctat cacgacatca gaatgagccg tgaggcgtgg
     3121 aatgcgttaa gcgatcaaga aaaacagcaa cgattggtgt cagcgtctga ttttctggat
     3181 ttcaattatc gtttttcagg cgaaaaagca gatcttactc aagagcgtga atttccacgc
     3241 aagggttttg aagctatcgg tattcctaaa gccgtgcaat ttgcggtatg tgagttggca
     3301 ttgcaagata acttgaccgc aaatcaagaa gctcaaatga gtagcgtaag agttggcccg
     3361 gtttcagtta attatgataa cagttcaccg caaaacagcg tgaatcggtt tgagtatgtg
     3421 aggcagttgc tttccacttg gttagatcga tcttctttcg gcacggttaa gttggagcgt
     3481 agttgatggc ggatttttac ggcaatttag ggaaaatctc ccattctttg attaagcaat
     3541 tcgggcagcc ttgtagtgta acaattaacg aacaaggcag atacaaccct gcaaccggtg
     3601 agcggaaaaa aggtagctca acggttcacg caggctattg tctgtttgat aatttggcgt
     3661 ttgatttccg caatcagcaa tctgtcagcg ttcaacaagg cgatgtgatg atttatctca
     3721 ctgccgattg caaaccggta ttaaatgctg tgattgaagc aaacggcgaa aaatggtcgg
     3781 tcatcaacgt ccagcctata cgcccagcag ctaccacgat gatttatcaa gtacaaggga
     3841 gaaaagccga tgggtagttt tcacgctcaa atcgcccgtt ttgtggaaca aacgcaacaa
     3901 aaagcggata gggcgttaag ggtaattgcc ctgcaaacct taagtggggt gcagaagaaa
     3961 tcgcctgtcg ataccggaca acttcggcgt agctggacgg tggcattaaa tggtttcccg
     4021 agcagttata acggcagtca aaccgtgctg aatagtgcta aattcggcga tatgattgtg
     4081 attgctacca ataaacctta tgcaccaatg ctcgaatatg ggctttaccc gaacccacca
     4141 aaaaaaccga ccggcaagac caaaaacggt tattcaatac aagcacctaa tggaatggtg
     4201 cgaattaccg tgcaggaaat gcaagccttt atccgcaata atccacaatt aggagtaagc
     4261 tgatgcgacc gattatccgc tcggtattag agcaacatct tgccgaactc aatccgttta
     4321 atacggtttg ggagggcatc atcgacagcc cgaaactgcc gtatcaaacc gtatcaatga
     4381 cgacttcaac caacgaaacc gccacgattg gtaatacgcc gaaagccaaa cagagcggtt
     4441 ttttgcaggt cacgctttac tatccgctag atcaggggac aaaggcgatt gaggaacgag
     4501 ccgatgagat cttccgccac tttttcgggc aggtctggat tgaggaaaat gtgcaaattc
     4561 tggtgcaaca gccaccgctt gtagcctcac tttatcgact ggataacagc attgccttac
     4621 ccgtcaccat ctattttacc gcttatgaac tataggagaa cccaatgtct attctaggca
     4681 attcacaagg cacaaaacgt acggtcgttt ttgccaaaga gagttctttc ggcacgaaag
     4741 ctgctccgac aggcgcaaaa attatgcctc gtgttgaaac ctcactcaat accaattttg
     4801 aaagttatca atcggaagag atcagagatg atttacaacg ctccgcctct gtgatcggct
     4861 ttgaaaaggt cgaaggcgat attaaaggcg aactagccgc cggacaatgg gcggcatttt
     4921 ttgcggctgc attacgtggt tcatttaccg ctcaggcgaa aaagccgatt attaccaaaa
     4981 ccacagacgg caccggcgag aaaaacggta aaattttgat tgtgccgaaa accagccaca
     5041 cctccgacag tttcacgctg gaagatgtct tcaaagacat caacgtcagc cgccagtatt
     5101 tgggatgtcg tgtatcgaaa ctcagccttg atgtgcaacc aaacggtatt gcctcgatta
     5161 cagtctcatt tttagggcaa cgaggcgaag aaagtacaac agcctatttt accaatccca
     5221 ccgatttaac ccaatccggc aagctggcag gggtaaaagg ctcattgcag gtcaataaac
     5281 gcccagtcgg gttagtgacg gcctttaaac tggacattga tttaggggca agcagtgagc
     5341 cggtattagg ggcaaaatac gcccctgatg tctttatcgg tacagtggca gtgagcggat
     5401 cgtttacgat gtacttccaa gacaagacga tgattgatgc ggtgcgtaac ggcacagatt
     5461 tatcccttgc tttgcggatg gatgccgaag agcgtaccaa taccgactat ttaaccatta
     5521 tcttaccggg tgtcaaagtg acctcaaacg agttggacga tggggcgaaa aacattatgc
     5581 agacactcaa ttttgacgca ttccctgcgg tatgggattc agcaagccaa atcgacaacg
     5641 tgctaaaagt cgccaccaca atgattgtgc aagacagcct cgcttaatcg aaattttgca
     5701 aaaaaataac cgtatctaac cgcttgccct caaacaagcg gtctttttta tccccaattt
     5761 acaaaaggaa acccaaatga atttaaacgc attaagtaaa gaaaaacagt tagccgaaca
     5821 ccgttttgag ctggttcacc ctgttagtgg cgaaccgctc ggcgtgttta tgagcgtcgt
     5881 gagtgctaag agtgataagg ctcaacgctt tgccgaaaaa caattacgcc aagaacagat
     5941 gagagagctg gaaaatgcca aatcccgcaa gccgaaattc aaaggcttag gcgatatgat
     6001 tgccgaaagt cgtgcaatgg cgattgaccg cttaaccggc tgggagaatg tcgaatggaa
     6061 cgaaaaaccg ttggagtttt ccgaccagaa tgcggagcta cttttaaccg aatgtgattg
     6121 gattgtggag caaatctttg agcattcgaa cgatctggga aagttcttga agagctaatt
     6181 gatgatttac aggtgtatgc agagaaaacg tttaagttgg ataaaaaact taaaggcagc
     6241 gaaagtacct tgcgagagca tttgcaggca atcgaaatgc aaactggcat tacaccagaa
     6301 gaattgcata accctgaacc cagtccagcg gtggcctatc tgctgggctg gttcttcgag
     6361 ttggcaatga gccgacaatc gggtatgagc ttaaacccaa ttagctacac tgaaattgat
     6421 gcgtggagca ggctttttgc tcgtcatctc gaacaatggg agatcagcgt aattaagcag
     6481 cttgatatga tttacttgaa cgtacaacaa acggagtagt atggatactt tcaactttca
     6541 gcccaattgg gggcttaacg tacagagaaa gccagaagtg atgaaaatcg cctttggtga
     6601 cggttacgaa caagtcgccc ctaaagggtt aaaccataat ctccgcactt attccgctgt
     6661 tttcaccggc acagaagcac ggattaaaca gattgaggca ttttttgaac gacaaggcgg
     6721 ctataaggct tttctttgga caccttacgg cgggaaagag gggaaattcc gctgtgaaga
     6781 gtgggaggta agcataagca gcgggaaatg gacgctgtcg gcgacgataa gagaagtgtt
     6841 gtagtttttt ctgattttgt gaactggttc acactctgtg ggttggatat aggttagaat
     6901 ttaagtctaa tttgcttaac ggagtctttt atgaaaaaaa tttttgtttc agccttattg
     6961 ctcacttgct cgttatctgc acttggaaat tcctcgtatg agttgattat tgactcaatg
     7021 caagatcttg aaaagcataa acccgaaatt ctatctatac cagaagcgga tagaaatact
     7081 tttattcgtt atatgatgcg aaaagaactg cggataaagt taaatcaagg cagattagcg
     7141 gatggtctaa ctgtgagaga agctattcaa aatcaacagg catttgaaaa agatagaaat
     7201 agcaaaatgc agcaggagaa gcaaaaaaaa gaaacagaga aagcagaaaa agaaaaagct
     7261 gttacggatt ttaaccaaaa atttgagcta tccattacag gggcgagaaa ggcaaaaaat
     7321 gcactaaatc aagatatttt gcttgtttca ttaagtctaa aaaataactc agataagcca
     7381 attatcgctg tacaaccgga tattcaattt gtactgggtg aagataaaca aactatttca
     7441 tccatgcgtc caaaaaaatt tgaaaacggc attccgccta atggctcaga gatttttgag
     7501 tttgggtttg atttagatga tatgtttgct ttgaaagcag aaaaaaatat agagaaattg
     7561 agggcagtct tccaaacagc agaagttctt tttgctgatg gaacaactga aaaacttcaa
     7621 tagtaattgg taaatcttca ttggccgctt gtagaaatac aggcggtttt tttattggag
     7681 caagctatgg cagatttagc aacactggcg attaaaatcg aaacagaagg cgcaagccga
     7741 gcaagtgcag atctaagtaa ggtcgagcaa tctgcaaaga acacagagaa agccgcagat
     7801 aatctgacgc gcgcaatggg aaatttgaag cggattttag cgattgcagg gctggctggt
     7861 gggttatccg cttacctaga tatggctgaa agaatgcagt cgttaaacgc acagcttaaa
     7921 tttgttacta actctcaata cgaattcaat agcgcacaaa aagagctttt tgcgattgcg
     7981 cagaatacgc gcgctagttt ggaaagcacc acgcaacttt atattaaatc ttctcaggca
     8041 ctgaaagatt acggctacgc gcaaaaggat attctcacat ttaccgaaac cattaacaaa
     8101 gcaatggcag ttggcggggt taagccacaa gagcaagcta gtgctttact ccaattatca
     8161 caggcattag gttcagggaa actgcaagga gatgaattcc gttcaatttc agaagctgca
     8221 ccgattattt tagatacgct tgccgagtat atgggtaaaa gtcgcgatga agtaagaaaa
     8281 ctcgcatccg agggtaagct caccgcagac ttactgttca atgcgtttaa cggttcaagc
     8341 gctaaaatta ccgaaaaatt taatcaaatg ccgctaacat ttggcggtgc aatgcagcag
     8401 ttgcagaatg ccacattgaa atttatcggt gacctagata gtgcaaatgg cttgagtgga
     8461 atgttagctc aagccattag ttttttagca cagaattttg atgtgttagg caaggctttg
     8521 atttatgcaa ccggtgctta tattgcgttt aatgcagttg cctttgctca gaattttgca
     8581 aaagcaaccg gtggtgtcgg tttattaagt agtgcatttg gttatttgac tacgatgatc
     8641 cgaggtgcaa ctgttgcaat gatggctaat cctatcgagc ttttaacaac tgcaattatt
     8701 ggggctgctt ttgtatttga tgcttttatc agtgatatag aggtaggtgc agcaaccttt
     8761 gatgcgactt ggggagatgt tgcgacagct gtgtgggatg attttaaaga tttaacttca
     8821 gatgcggcag attggttagt cactcaatgg gataaagcaa ccgccagtgc agcgagttcg
     8881 ttcagttcct taggtgttga tattggctca gtatgggaga cggtgcaatc cattacgaaa
     8941 acattgatca atagtattat tggtctgttt gttggagctt atcaagccat cgtgcttgct
     9001 tggaataatt ttcctgccac aatggaaaac ttgggtgtga tggcggtaaa tgctcttgtc
     9061 gatttggttc aaaaaggcat caatagcatt attcagctta tcaaattacc gattgagctt
     9121 ttaaacagag cctcatctac ttttggtgga ggaaatctga ttgatacatc aggttggaag
     9181 gcaagcttag atgattttaa actaaaggca agccgcgagg cagaagttgt tggtcaatca
     9241 attagccaag catttgcgga tagtatcacc acagattaca taggcaatgc agttaattca
     9301 gtgagtgact atattgatgg agcggcagtt cgtgggcgct taaaacgtca attagatgaa
     9361 accgcctctg acgatcaagg tacgcagtca ccagctaaaa aaacattagc tggaaataat
     9421 aactctgcta gtgactggaa aaactacctc aatgaacttg aacgagccaa tgccgatgga
     9481 ttagcccgta ttgcacttga gcaagatcgc tcaatgcgtg aaatgttgga aaaggcgaag
     9541 aaagccggtg caagtcacgc tgaaattgaa aaagccaaaa tgctgattac agaacgttat
     9601 gctaaagagc gaatggaaat tgccgagaag tatgtgccga gtctgaagtt tgaggaagaa
     9661 ctcaaagatc agattaagga aattgatgaa ctgcaaaagt taaacctgat taccaccgag
     9721 caagcaggtc ttgcgaaaga ggcgttagca ggcaaatatc agccggtagt tgaaatccaa
     9781 cagcaataca tcaatcaact gagagagatt gaagcattaa aacaagccgg tgtattaagc
     9841 aaacctcaag cagaagtagc ggccgaaaaa gccaaatggg acgccggcac acaaaaagcc
     9901 aaaatcgcag gtaaaaacgc cgttagccct tatgaaaaat ggaaatcgga atttgaccca
     9961 atgcaggcaa ttcaaaatga gcaagcctca aagttggcag aagtgcaatc gatgtacgat
    10021 cagcatttga tccaatatga ggattttatc aacgctaaag cacaaattga tgctaaggcg
    10081 actgctgata ctcatcaact gtttatgaac agtattagtg ggtttggttc ggcgatagat
    10141 acgatgttag gcgtgatgaa aaacgcaggg caagagcaat caggtattta ccgcacgatg
    10201 tttgcgatgt cgaaagcgtt tgctattgcg gaatcaattc tgaacttgaa tcgtgcaatt
    10261 actcaagcaa tgagcgatca aacagctatt acaccggctc aaaaatttgc aaatatggcg
    10321 gctattgcta gtgccggggc gagcttgatt tctaatatcc agtcagtctc attaggcggt
    10381 atggctcaca gcggtattga caatgtgccg aaagaaggca cttggttgct ggataaaggt
    10441 gagcgtgtag tggatagtcg tactaaccaa gacttaaaaa cctttttatc caatgctaat
    10501 cgtggcggtg caagtggtca aagatcggcg ataaatgtca aaattattaa taacgggcaa
    10561 cccgtagagg cgaaagtcag cagtgaagag actgtagatg gcaatatcca aatgacggtg
    10621 gaactgctga agaaaatgga tcaaatcgcc gatcaacgct accgtagaaa tcagttaaat
    10681 gatttaagaa caggtggaac attatctaaa taggtgaagt atgccaatta gtatttctaa
    10741 ccaaatgaaa ctagatcttg ccaaacttga gcaaaatgca atgctggact tgtacgaggt
    10801 tgatttgcgc aatctcaaag ataaaagtgg taatgctggc agagtttacc gcttttattc
    10861 tgggctaaat gaactcaaaa caagtatcgt ctggcaaggg cggacttacg atccgtatcc
    10921 tattgaggca agtggatttg aacgaaacag tagcggcccg agcaatcgcc cgactctcac
    10981 gctatcaaat ctgtttggtt taattaccag tattgcgaat cagtttgacg aatgtatcgg
    11041 tgcgattgtc cgccggcatc aggtttatgc tcaatattta gatgccgtga attttgccgg
    11101 gggtaatgcg aaagctgatc caaatcagga aataatcagt cattttgtga ttgagcagct
    11161 taccagcctc acccgagaaa cggcgacctt tacgctggca ttaccgattg aaaccgataa
    11221 tgccaagatc cccagcagaa ttattatggc ggacacctgc acttggattt accgctcggc
    11281 ggaatgtggc tacaccggca agcagtattt tgatgaaaaa gacaaatcaa cctcagaccc
    11341 aaaagcagat aaatgtagtc attgcctgaa cggttgtgaa ttgcgaggca atcagcgtaa
    11401 ttttggtgga tttgtatcgg tgaataaatt ggggtaatga atggaattgg aacaacaaat
    11461 aatcgactac gccctccaac acgaaccgca cgaaatgtgc ggctttgttg tttttgacgg
    11521 caataaaaat cgatttatcc cttgcgaaaa ccaagcggaa gacaaagcca attactttga
    11581 aatctccgat cttgattaca tcaaagcaga agaaaaaggc gaactggtag cagtagtgca
    11641 ttcgcaccct gagccaaacg gcaagccggt actctctacc cttgatcgca aaatgcaggt
    11701 acagaccggt ttagattggt ggctagtgca taatcggcaa atccataaat tccgcaatgt
    11761 accacattta atcgggcgtg agtttaaaca cggcacaatg gattgctaca cgctctaccg
    11821 tgatgcctat atgcttgccg gttacgagat ggacgagttc gagcgacaag atgactggtg
    11881 gcataccggg caaaatctat atttggataa tattcaaggg caaggtttcg agcgagt
//
LOCUS       DANLUQ010000067        11884 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.067, whole genome shotgun sequence.
ACCESSION   DANLUQ010000067 DANLUQ010000000
VERSION     DANLUQ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11884
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.067"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            3..740
                     /gene="tsaA"
                     /locus_tag="PXB27_001420"
     CDS             3..740
                     /gene="tsaA"
                     /locus_tag="PXB27_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HDL5346199.1"
                     /translation="MNSTSNQLTPIQLNPIGFVESPYDEKFSVPRQPNLVAEGKGILR
                     LIPPYNTPDAVRGLEKFSHIWLIFQFHHIPEREWHATVRPPRLGGNERVGVFASRATH
                     RPNPLGLSKVKLEAVVAGGSEVYLKLGSVDLVNGTPIFDIKPYIAFADSEPQAVSGFA
                     QQKPQARLAVRFSEEAKQAVEFCRNFAKFGIEQPLSFLADVIAQDPRPAYQQGKPSER
                     VYGMNLAGYNIRWQIENDTQAIVLSIE"
     gene            798..1517
                     /locus_tag="PXB27_001421"
     CDS             798..1517
                     /locus_tag="PXB27_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253715.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HDL5346200.1"
                     /translation="MKLKNFLTILPLAVATAVTSAETHTVEHKDKPASFNFSTEVSRT
                     VEKDVMQASVYSQKSGNSLPELKKFVSENLNRVLAEAKKHPSIEVQAEGVTNHVNYNN
                     KGNIDGWDTSGSIQLKSKDFDAMAAVLENLGKDVAIRYIDFSVSPEKMASLEDEMTLE
                     IISKFQHKADVIQKALGAKTYKLVNINIQTPNDGGRPYYEHRAMYAVSSMAQSKSMDE
                     IPLEAGKTTISATASGSVEFE"
     gene            1705..2391
                     /gene="aqpZ"
                     /locus_tag="PXB27_001422"
     CDS             1705..2391
                     /gene="aqpZ"
                     /locus_tag="PXB27_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HDL5346201.1"
                     /translation="MKKYFAEFFGTFWLVFGGCGSAVLAAGIPELGIGYAGVSLAFGL
                     TVLTMAYAVGHISGGHFNPAVSIGLLVGGRFNAKDLVPYIIAQVIGAIAAGAVLYTIA
                     SGVPTFDATAGFAGNGYGEHSPHGYSLTAALLIEVVLTAFFLIIIMGATDKRAPAGFA
                     PIAIGLALTLIHLISIPVTNTSVNPARSTGVALFQGSWAIEQLWLFWVAPIVGAIIGA
                     VVYRFIAEEK"
     gene            2463..2603
                     /locus_tag="PXB27_001423"
     CDS             2463..2603
                     /locus_tag="PXB27_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346202.1"
                     /translation="MKKLIKVCGLISATIFLSGCLVTSVVGGVVGTAVDVVDTVTPDI
                     ID"
     gene            2706..3299
                     /gene="slyD"
                     /locus_tag="PXB27_001424"
     CDS             2706..3299
                     /gene="slyD"
                     /locus_tag="PXB27_001424"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL5346203.1"
                     /translation="MNITKDLVPSIAYQVRTQEGVLVDEAPVNQPLEYLHGHNNLVEG
                     LEKALEGKAVGDVFEVRVKPEEGYGEYNDNMVQRVPKDVFMGVDELEVGMRFIADTDL
                     GPLPVVITEVDGDEVVVDGNHMLAGQELLFSVEVVGIREATAEEIAHGHVHGGHEHGC
                     GCGGHDHHHDHDGCCGGHDHDHHHHHGHGGCGCGGHH"
     gene            3444..4976
                     /gene="ilvA"
                     /locus_tag="PXB27_001425"
     CDS             3444..4976
                     /gene="ilvA"
                     /locus_tag="PXB27_001425"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HDL5346204.1"
                     /translation="MMNETQHSQSAIDYLRSILSSNIYDLAQVTPLQKMEKLSERLGN
                     SIFIKREDRQPVHSFKLRGAFAMISNLSKAQKEAGVIAASAGNHAQGVALSAKHLGLR
                     ALIVMPQNTPAIKVDAVRGYGGEVLLYGANFDEAKAKAIELSTELGMTFIHPFDSPAV
                     IAGQGTIGLELVQQKPDLDYVFIPVGGGGLIAGIAVLIKQVMPNIKVIGVESKDSACL
                     YHALKQGQPTDLERVGLFADGIAVKRIGDETFRLCQQYVDDVVLVDNDEICASMKLIF
                     ENVRAISEPSGATSLAGLKKYVKEHQLQDKNLACILSGANVNFHTLRFVSERCEIGEK
                     REALLAVTIAEQKGSFLKFCEILGNRAVTEFNYRHSDDKQACIFVGVRISGSAEKVEI
                     IKDLQQNGYDVADLSDDDIAKTHIRYMVGGRASSIQYEQLYSFEFPEQKGALLKFLQM
                     LTNWDISLFHYRAHGADYGDILAAFALNRGDEVELNRHLEQLGYRFQNVSESPAYQYF
                     LK"
     gene            5002..6336
                     /gene="glmM"
                     /locus_tag="PXB27_001426"
     CDS             5002..6336
                     /gene="glmM"
                     /locus_tag="PXB27_001426"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015939592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDL5346205.1"
                     /translation="MARKYFGTDGVRGEVGKFPITPEFALKLGWAAGKVLATQGTKKV
                     LIGKDTRISGYMLESALEAGLTAAGLSAVFVGLMPTPAIAYLTRTFRAEAGIVISASH
                     NPYYDNGIKFFSSVGEKLPDEIEEAIEAMLDKPMDCVDSANLGRASRINDAAGRYIEF
                     CKGTFPSELSLEGYKIVVDCANGATYHIAPNVMRELGAEVLEIGTKPDGLNINEKCGA
                     TDIKALQKVVVEAGADVGLAYDGDGDRLIMVDHLGNKVDGDQILFILAREALRSGKLQ
                     GGVVGTLMSNMSLELALKELAIPFSRANVGDRYVLEQLKEKGWKLGGENSGHIIVLDK
                     NTTGDGIIASLQVLAAMASHKLSLNDLAKAVPLFPQVLLNVRFEKGAANPLESNEVKA
                     VAADVEKRLAGKGRILLRKSGTEPLIRVMVECEDAVLAQSCAEEIVEAVKRN"
     gene            6481..7140
                     /gene="rpiA"
                     /locus_tag="PXB27_001427"
     CDS             6481..7140
                     /gene="rpiA"
                     /locus_tag="PXB27_001427"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HDL5346206.1"
                     /translation="MDQQEMKKIAAQAALKFVKPDTIVGVGSGSTVNCFIDALASMKD
                     EIKGAVAASKASEQRLRDIGIEVFSANEVTELDVYIDGADEITPQGAMIKGGGAALTR
                     EKIVSSLAKKFICIVDKSKQVDVLGSTFPLPVEVIPMARSYVARQLVALGGSPEYREG
                     VVTDNGNVILDVHNFKIMEPLKMEHTINNIAGVVTNGIFAQRFANVTIVGTPEGAVIL
                     E"
     gene            7177..8406
                     /gene="serA"
                     /locus_tag="PXB27_001428"
     CDS             7177..8406
                     /gene="serA"
                     /locus_tag="PXB27_001428"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDL5346207.1"
                     /translation="MAKISLDKSKIKFLLLEGVHQNAIDVLLAAGYTNIEVHKKALDG
                     QELIDAIKDAHFVGIRSRTFLTEEVLAQAPKLIAIGCFCIGTNQVDLNAAKRRGIPVF
                     NAPFSNTRSVAELVLAEMILLMRKVPTANAEVHRGVWNKSAAGANEVRGKKLGIVGYG
                     HIGSQLSILAEAIGMQVYFYDVENKLPLGNAQQVPTLEHLLSTCDMISLHVPEIDSTK
                     NLMSAERIAQLKEGSVLINAARGTVVDLDALAARIEDGSLRGAAIDVFPEEPASINDP
                     FVSPLCKFDNVILTPHIGGSTSEAQANIGTEVANKFVKYSDNGSTVTAVNFPEVSLPL
                     HTNTKRLLHIHENRPGMLNQINQIFVESNVNIAAQYLQTDAAIGYVVIDVESDNVDEA
                     LQRLKAIEGTIRARVLY"
     gene            complement(8484..9002)
                     /locus_tag="PXB27_001429"
     CDS             complement(8484..9002)
                     /locus_tag="PXB27_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HDL5346208.1"
                     /translation="MKKLTIVALTCLFSTHLLAAPVGNTFTGIGVGVDVTTTKYKVEG
                     VKGKDSTGPALVVDYGIEQGNNFVGVVQGKAKVGSTKVFSDVKQKNKYTIAYQQGYRV
                     GSDLLPYVKVDASSSKIGNETYRGFGFGAGAKYAVSSDVELGAEYTRDNLKRSGTKLK
                     GNAFTANATYRF"
     gene            complement(9022..9744)
                     /gene="arcA"
                     /locus_tag="PXB27_001430"
     CDS             complement(9022..9744)
                     /gene="arcA"
                     /locus_tag="PXB27_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HDL5346209.1"
                     /translation="MKIPQILIVEDELVTRNMLKSIFEGEGYQVFQASNGEEMHSVLS
                     TNEINLVLMDINLPGKNGLILAREIRENMKLPLMFLTGRDNEIDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLQRTMIDKENDVVKEQSEIESYRFNGWILDINSHSLISPEGEV
                     NKLPRSEFRALLHFCQHPGKIQTREDLLKKMTGRELKPHDRTVDVTIRRIRKHFEDHP
                     NTPEIIVTIHGEGYRFCGNIEP"
     gene            complement(9877..10569)
                     /locus_tag="PXB27_001431"
     CDS             complement(9877..10569)
                     /locus_tag="PXB27_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL5346210.1"
                     /translation="MILFAGDPHGSYEHLYPILQQQTEEVALVILGDLQLTTPDELDR
                     LSQYCDIWFIHGNHDSKTVAAFDAIWNSSWKERNIHGKVVDIQGIKIAGIGGVFRGHI
                     WMPPNRPMFFDPIHFCQYSAPEKIWRGGIPLRHRSSIFPSDFEALENEKADILITHEA
                     PKPHPQGFSVINQLARKLGASKIFHGHYHDNFNYGNLNVSRECAIYNIGFRSLADING
                     NYLILGVDDREK"
     gene            complement(10587..11861)
                     /gene="nadR"
                     /locus_tag="PXB27_001432"
     CDS             complement(10587..11861)
                     /gene="nadR"
                     /locus_tag="PXB27_001432"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HDL5346211.1"
                     /translation="MTDFAYLQQKRKQLKLKVNDICEQAGVTRAYFNQLVSGKIKNPS
                     ANKLKALHSVLDIVEDSNQRSGVIFGKFYPIHTGHINMIYEAFSKVDVLHVIVCTDSE
                     RDLRLFQESKMKRMPTNEDRLRWVQQIFKYQQKQIFIHHLVEDGIPSYPNGWEDWSGR
                     VKALFEEKSINPTIVFSSEIQDKESYEKYLNLEVQLVDPTRESFNISATQIRNNPFQY
                     WRFIPKEVRPFFVKTIAILGGESSGKSVLVSKLANVFNTTSAWEYGREFVFEQLGGNE
                     QAMQYSDYPQMVLGHQRYVDYAMKHAHKVAIIDTDYITTQAFCIQYEGKAHPFLDSMI
                     REYPFDVTILLSNNTKWVADGLRSLGSKKQRQQFQHLLKKLLEKHHIPYIKIESPSYL
                     DRYNQAKEVVEAILNEEPIPLQFKQYNIHEFE"
BASE COUNT         3468 a         2253 c         2637 g         3526 t
ORIGIN      
        1 taatgaactc tacctcaaat caactaaccc cgattcaatt aaaccctatc ggttttgtgg
       61 aaagccccta tgatgaaaaa ttttccgtgc ctcgccagcc gaatttggta gcggagggga
      121 aaggcattct acgtttaatt ccgccttaca atacccccga tgcagtcaga ggcttggaga
      181 aatttagtca tatttggttg attttccaat tccatcacat tcccgaaaga gagtggcacg
      241 ccaccgtcag accgcctcgc ttgggcggga acgaacgggt cggggtcttt gccagccgtg
      301 ccacccatcg tccgaatccg cttggcttat cgaaagtgaa gttagaagcg gtagtggcgg
      361 gggggagtga agtttatttg aaattaggca gcgtggattt agttaacggc acaccaattt
      421 ttgacatcaa gccttacatt gcatttgcag atagcgagcc gcaagcggtt tccggttttg
      481 ctcagcaaaa accacaagca agattagcgg tgcgttttag cgaggaggca aaacaagctg
      541 tcgaattttg ccgaaatttt gcaaaatttg gcatcgaaca gccgcttagt tttcttgccg
      601 atgtcatcgc ccaagaccct cgcccggcgt atcaacaagg gaaacctagt gagcgagttt
      661 atggtatgaa ccttgccggc tacaatattc gctggcaaat tgaaaatgat acacaggcga
      721 ttgtgctttc gattgaatga actttcctac gcaagtgaga tctaagaaat gatttttaac
      781 ccttttaagg actgattatg aaattgaaaa attttcttac cattctaccg cttgcagtcg
      841 caacagcggt aacttcagcg gaaactcata ccgtggaaca taaagataaa ccggcttctt
      901 ttaatttctc aaccgaagtc tctcgcacgg tggaaaagga cgtgatgcag gcaagtgtgt
      961 atagccaaaa atcaggtaac tccttgccgg agctgaaaaa atttgtatct gaaaacctga
     1021 atcgtgtatt ggctgaggca aaaaaacatc catcgattga agtacaagct gaaggtgtga
     1081 ctaatcacgt taattataac aacaaaggga atattgatgg ttgggatacc tctggtagca
     1141 ttcagttgaa aagcaaagat tttgatgcaa tggcagcggt gttggaaaat ttaggtaaag
     1201 atgtggcgat tcgttacatt gattttagtg tatcgccaga gaaaatggcg tcccttgaag
     1261 atgaaatgac gctggaaatt atcagcaaat ttcagcataa agccgatgtg attcaaaaag
     1321 ccttaggggc gaaaacatat aagctagtta atatcaatat tcaaactcca aatgatggtg
     1381 gtagaccata ttatgaacac agagcaatgt atgcagtttc atcaatggca caaagcaaat
     1441 ctatggatga aatcccgtta gaagcaggta aaaccacaat ttcagctacc gcttcaggaa
     1501 gtgtggaatt tgaatagaaa tgtatagatt tgtatagagt tgtattttta ctttaattgg
     1561 aggaaaaaat cggtctatac tatcaattaa gaaatatgcg tctttacttt ttttagtata
     1621 ctcatatttt attggctatg ttttatagca ttcggaaagt taatacactt atgtgtgatt
     1681 caccctttag ggtaaggata aaaaatgaaa aaatattttg ctgagttttt cgggacattt
     1741 tggttagtgt ttggtggttg cggcagtgca gttttagcag caggcattcc tgaattagga
     1801 atcggttatg caggcgtttc gttagccttc ggtttaaccg tattaacaat ggcgtatgca
     1861 gtcggtcata tttcaggtgg tcacttcaac ccggcggttt caatcggttt attagtcggc
     1921 ggtcgtttta acgctaaaga tttagtgcct tatatcattg cccaagtaat cggtgcgatt
     1981 gctgccggtg cagtgttata caccattgct tcaggtgttc cgacttttga tgctacagca
     2041 ggctttgccg gcaacggtta tggtgagcat tcgccacacg gctatagctt aacagcagca
     2101 ttattaattg aagtggtatt aacggcattc ttcttaatca tcattatggg tgcaaccgat
     2161 aaacgtgctc cagcaggttt cgctccgatt gctattggtt tggctttaac cttaatccac
     2221 ttaatcagca ttccggtaac caatacctca gtaaacccgg cacgttcaac cggtgtagcg
     2281 ttattccaag gcagctgggc gattgagcaa ttatggttgt tctgggttgc tccgattgtc
     2341 ggtgcaatta tcggtgcggt agtttatcgc tttatcgctg aagaaaaata atttaagcga
     2401 ataaatatga tgcagctcta gtcttgatta gggctgtttt ttgtttttga aaggatttta
     2461 aaatgaaaaa attaatcaaa gtatgtgggc tgatttcagc aactattttc ctaagtggtt
     2521 gtttggtaac ttcggtggtt ggtggagtgg tgggaaccgc agttgatgtg gtagacaccg
     2581 taacccctga tattatcgac taatttactg acaagcggtt aaaatttcca gatagattgc
     2641 aaatataaaa taaagcgtta caatggcttg ctattttata aaaccgatga attaaaggat
     2701 tcaatatgaa cattacaaaa gatttagtgc caagcattgc atatcaagtg cgtactcaag
     2761 aaggcgtatt agtagatgaa gcaccggtaa accaaccgtt agaatattta cacggtcaca
     2821 acaacttggt agaaggctta gaaaaagccc ttgaaggcaa agctgtaggc gatgtgtttg
     2881 aagtgcgtgt gaaaccggaa gaaggttacg gcgagtacaa cgataatatg gttcaacgtg
     2941 taccgaaaga tgtatttatg ggcgtcgatg aattagaagt gggtatgcgt tttattgcgg
     3001 acacggattt aggtccgcta ccggtggtaa ttaccgaagt agatggcgat gaagttgtgg
     3061 tggacggtaa ccatatgtta gccggtcagg aattactttt ctctgtggaa gtggttggca
     3121 ttcgtgaagc gacagcggaa gaaatcgcac acggacacgt tcacggcgga cacgaacacg
     3181 gttgcggttg tggtgggcac gatcatcatc acgatcacga tggttgctgc ggcggtcacg
     3241 accacgatca tcaccatcat cacggacacg gtggctgtgg ttgcggtggt catcactaac
     3301 tttgtgattt cccctctccc gcaaacgggc gagaggaaat attccctgtt aaagccattg
     3361 ctaaataacc aaacaagcgg tcaattttcc ttggaaattt accaatgtct ttatctcaat
     3421 ctaacaataa ataacctttc aaaatgatga acgaaacaca acattcccaa tccgctatcg
     3481 actatttacg ctcgatttta agctcaaata tttatgactt agcgcaagtg acccctttac
     3541 agaaaatgga aaaactgtct gagcggttag gcaactctat ttttatcaaa cgtgaagacc
     3601 gacagccggt tcacagcttt aaattgcgag gtgcttttgc gatgatctca aatttatcca
     3661 aagcacaaaa agaggctgga gtcattgcgg cttcagcagg caaccacgca caaggggtgg
     3721 cactttctgc caaacattta ggtttaagag ccttgattgt aatgccgcaa aacacaccgg
     3781 caattaaggt tgatgcggta cgtggctatg gtggtgaagt gttgctttat ggggcgaatt
     3841 ttgatgaagc taaggcgaaa gcgattgagc tttccaccga attgggtatg accttcattc
     3901 acccgtttga cagcccggca gtgattgcag ggcaaggcac aatcggctta gaactggtgc
     3961 aacaaaagcc ggatttagat tatgtattta ttccagtggg cggtggcggc ttaattgccg
     4021 gcattgcggt tttaattaag caggtgatgc cgaatattaa agtgattggg gtcgaatcta
     4081 aagattctgc ttgcttatat cacgccttaa agcaagggca accgaccgat ttagaacggg
     4141 ttgggctttt tgctgacggt attgcggtaa aacgcattgg cgatgaaact ttccgcctgt
     4201 gtcagcaata tgttgatgat gtggtgttgg tggataatga tgaaatctgt gcctcaatga
     4261 agttgatttt tgaaaatgtg cgtgcgattt ccgagccgtc cggggcaact tcccttgccg
     4321 gtttgaaaaa atatgtgaaa gagcatcaat tacaagataa aaacttagct tgcattcttt
     4381 ccggggcgaa tgtgaatttc cacaccttac gttttgtgtc agagcgttgt gaaatcgggg
     4441 agaaacgtga agctttattg gcagtcacca ttgctgaaca gaaaggtagt ttcttaaaat
     4501 tctgcgagat tttaggcaac cgtgcggtaa cggaatttaa ctatcgccat tccgatgata
     4561 aacaagcctg tatttttgtg ggggtgcgaa tttcaggctc ggcagaaaaa gtagagatta
     4621 ttaaagatct gcaacaaaac ggctatgatg tggcagattt atcggacgat gatattgcca
     4681 aaacccatat tcgttatatg gtaggcggta gagcaagctc cattcaatat gagcaactct
     4741 atagctttga atttccagag cagaaagggg cattgctaaa attcttacaa atgctgacta
     4801 actgggatat ctcgttattc cattaccgag ctcacggagc cgattatggc gatattcttg
     4861 ccgcatttgc attaaacaga ggcgatgaag tagaattaaa ccgacattta gaacagctag
     4921 gttaccgctt ccaaaatgtg agcgaaagtc cggcatatca atatttttta aagtaaattt
     4981 ttatcaatct taggaattag aatggctcgt aaatatttcg gcacagacgg tgtgcgtggt
     5041 gaagtaggta aatttccaat tacacctgaa tttgcattaa aactcggttg ggcagcaggt
     5101 aaagtgttgg caacccaagg cacgaaaaaa gtattaatcg gtaaagatac ccgtatttcc
     5161 ggttatatgt tggaatcggc tctggaagcc ggtttaaccg cagccggttt atcagcagta
     5221 ttcgttggtc tgatgccgac tccggcaatt gcttatttaa cccgtacttt ccgtgcggaa
     5281 gccggcattg tgatttctgc ctctcacaat ccttattacg ataacggcat taaattcttc
     5341 tcgtcggttg gtgaaaaact acctgatgaa attgaagaag cgattgaagc aatgcttgat
     5401 aagcctatgg attgcgtaga ttcagcaaat ttaggtcgtg ccagtcgtat taatgatgcc
     5461 gcaggacgtt atatcgagtt ttgtaaaggc actttcccgt ctgaactaag ccttgaaggc
     5521 tataaaattg tggtggattg tgccaacggt gcaacctacc atatcgcgcc gaatgtaatg
     5581 cgtgagctag gggcagaagt gcttgaaatc ggtactaagc cggacggttt aaacatcaac
     5641 gaaaaatgtg gtgcaacgga tattaaagca cttcaaaaag tggtggtaga agcaggtgcg
     5701 gatgttggtc tagcttacga tggtgatggc gaccgtttga ttatggtcga tcacttaggc
     5761 aataaagttg atggcgacca aattctattt attttagccc gagaagctct acgttcaggc
     5821 aaattacaag gcggtgtagt cggtacatta atgagtaata tgagcttgga attagcttta
     5881 aaagagctag ccattccatt ctctcgtgct aacgtgggtg accgctatgt actagagcag
     5941 cttaaagaaa aaggctggaa actgggtggc gaaaactccg ggcatattat cgtgttagat
     6001 aaaaacacca ccggtgatgg tattattgcc tcactgcaag tattagcagc aatggcaagc
     6061 cacaaattaa gcctgaatga tttggcaaaa gcagtgcctc tgttcccgca agttctgcta
     6121 aatgttcgct ttgagaaagg ggcggcaaat ccgcttgaaa gtaacgaagt gaaagcggta
     6181 gcggcagatg tggaaaaacg cttggcaggc aaagggcgta ttttattgcg taaatcaggc
     6241 actgagccat taattcgagt gatggtggag tgcgaagatg ctgtacttgc tcaaagctgt
     6301 gcggaagaga ttgttgaagc agtaaaacgt aactaatgac aagcggtcgt ttttggtaaa
     6361 aaatttgcca aatatgaccg cttgttacat atatgtgtag aatagatgta gtagggcgaa
     6421 aatttttttc gccttgccga agtgggcaaa tgatgatttg ccctacaagt ggaggtaaaa
     6481 atggatcaac aagaaatgaa aaaaattgcg gcacaagctg cattaaaatt tgtaaaaccg
     6541 gacactattg ttggggtggg aagtggttca accgtaaact gttttattga tgcccttgct
     6601 tcaatgaaag atgagattaa aggagcggtt gctgcctcta aagcctcgga acagcgtttg
     6661 cgtgatatcg gcattgaagt attcagtgcc aatgaagtga cggaattaga tgtgtatatt
     6721 gacggagctg atgaaatcac gccacaaggg gcgatgatta aaggtggcgg ggcagcactt
     6781 acccgtgaga aaatcgtgag ttcattagcg aaaaaattta tctgcattgt ggataaatcc
     6841 aaacaagttg atgtgctggg ttcaactttc cctctgccgg tggaggtgat cccaatggct
     6901 cgttcttatg tggctcgtca gctcgtagcc ttaggtggtt cgccggaata ccgtgagggc
     6961 gtggtcactg ataacggcaa tgtgatttta gatgtacata actttaaaat tatggagccg
     7021 ttgaaaatgg aacataccat taataatatt gcaggcgtgg tgactaacgg tatttttgca
     7081 caacgttttg ccaatgtaac cattgtcggc acacctgaag gtgcagttat tttagaataa
     7141 tttattattg caaatacaaa tacttaggag caaaaaatgg ctaaaatttc tttggataaa
     7201 tccaagatca aattccttct gcttgagggc gtacatcaaa acgcaattga tgtactctta
     7261 gcggctggtt ataccaatat tgaagtgcat aaaaaagcat tagacggtca agaattaatt
     7321 gatgcaatta aagatgcaca ctttgttggt attcgctcgc gtactttttt aaccgaagaa
     7381 gtactggctc aagctccgaa attaattgcg attggttgtt tctgtatcgg tacaaaccaa
     7441 gttgatttga atgcggcgaa aagacgaggt attccggtat tcaatgcacc gttctcaaat
     7501 acccgctcgg tggcagaatt agtgctggca gagatgattt tattaatgcg taaagtcccg
     7561 actgcaaatg cggaagtgca tcgtggggtg tggaataaat cggcagcagg tgcaaacgaa
     7621 gtacgtggca agaaattagg tattgtggga tatggtcata tcggttcaca gcttagtatt
     7681 cttgccgaag ccattggtat gcaggtttat ttctatgatg ttgaaaacaa actaccactc
     7741 ggtaatgcac agcaagtgcc gacattagag catttgcttt caacctgcga tatgatttca
     7801 ctacacgttc cggaaatcga ctcgactaaa aacctaatga gtgcagaacg cattgctcaa
     7861 ttaaaagaag gctcggtgtt aattaatgcc gctcgtggta cagtggtcga tttagacgca
     7921 ttagccgctc ggattgaaga cggctcattg cgtggtgcgg cgattgacgt atttccggaa
     7981 gaaccggcat caattaatga cccgttcgtg tcaccattgt gcaagtttga taacgtgatt
     8041 ttaacgccac atattggtgg ttcaacctct gaagctcagg caaatatcgg caccgaagtt
     8101 gccaataaat ttgtgaaata ttcagataac ggttcaaccg tgacggcagt gaatttccct
     8161 gaagtatcct taccgcttca tactaatacc aaacgtttat tacatattca cgagaatcgc
     8221 ccaggtatgt taaatcaaat taaccagatt tttgtggaat caaatgtgaa tattgccgct
     8281 caatatttac aaaccgatgc agctatcggt tatgttgtaa ttgatgtaga gagcgacaat
     8341 gttgatgaag cattacaacg tttaaaagcg atcgaaggca cgattcgagc ccgagtatta
     8401 tattaaaagg caaagagcct gaatcacttc aggctctttt gtgttacaag cggttatttt
     8461 ttcagaaaac tttaccgcta agattagaaa cgataagttg cattcgcggt gaaagcgtta
     8521 ccttttaatt ttgtgccgct acgttttaaa ttatcgcgag tgtattctgc acctaattca
     8581 acatcgcttg aaactgcata ttttgcacca gcaccgaaac caaagccacg ataggtttca
     8641 ttaccgattt tgcttgagct ggcatctact tttacataag gtaataaatc ggatccaaca
     8701 cgataacctt gttgataagc tatagtatat ttattttttt gttttacatc gctaaatact
     8761 tttgtactac ctacttttgc ttttccttgt acaacgccta caaagttatt gccttgctca
     8821 ataccataat cgacaactaa tgccggacct gtggaatcct tgccttttac tccttcaact
     8881 ttgtattttg ttgtagttac atctacaccg acaccgatgc cagtaaatgt atttccgaca
     8941 ggagcggcta atagatgggt agaaaataag caagttaatg cgacaatagt taattttttc
     9001 ataaaattac tcctatcttg attaaggttc gatgttacca cagaaacggt agccttcacc
     9061 gtgaatggtg acaataattt ctggggtatt ggggtggtct tcaaaatgtt tacgaatacg
     9121 gcgaatagta acatcaacgg ttctatcatg aggttttagt tcccgcccgg tcattttttt
     9181 caataaatct tcacgtgttt gaatttttcc aggatgttgg caaaaatgta ataaagccct
     9241 aaattcacta cgtggtaatt tatttacctc accttctggt gagattaaac tatgactgtt
     9301 aatgtctaag atccaaccgt taaagcgata gctttcaatt tcagattgct cttttacaac
     9361 gtcgttttct ttatctatca tcgttcgttg caataaattt ctcgctcgga tagtcaattc
     9421 tcgaggatta aacggtttag tgatatagtc atcggcacct atttctaaac cgaggatctt
     9481 atcaatttca ttgtcgcgac cggttaaaaa cattaatggt agcttcatat tctctcgaat
     9541 ttctcgggcg agaattaatc cattttttcc cggtaagtta atgtccatta gcacaagatt
     9601 gatttcattt gtactcagaa cagagtgcat ttcctcgccg ttagatgctt gaaatacctg
     9661 atatccttct ccttcaaaaa tgcttttcag catattcctt gttactaact catcctcaac
     9721 aattaagatt tgtgggatct tcatctttta aacctctgat ttaatttata aattctgttt
     9781 aataacgttt atgttacaaa tttctcgcat tttacttata aatttatgaa tgggaagggt
     9841 aaaatgacaa tttgtttaat tcagtcaaat ttttggctat ttctctcgat catcaacacc
     9901 taaaattaga taatttccat taatatccgc taggctacga aagccgatat tataaatagc
     9961 acattctctg ctgacattta gattgccata attaaaatta tcatggtagt ggccatggaa
    10021 aattttgctc gcccctaatt ttcttgctag ctggttgatt acagagaagc cttgagggtg
    10081 tggctttggt gcttcgtgtg taattaaaat atcggctttt tcattttcca atgcttcaaa
    10141 atcagaaggg aagatcgaag agcggtgcct taatggaata ccaccacgcc aaattttttc
    10201 cggagcagag tattggcaaa aatggatggg atcaaaaaac attggacgat tgggcggcat
    10261 ccaaatatga cctcggaaga cccctccaat acctgcaatt tttattcctt gaatgtcaac
    10321 tactttaccg tgaatattgc gttctttcca gctactattc caaatagcat caaaggcagc
    10381 aacggtttta ctgtcatggt taccatgaat aaaccaaata tcacaatact gcgaaagccg
    10441 atctaattca tcaggagtag tcaattgaag atcgcctaga ataactaaag ctacctcctc
    10501 cgtctgctgt tgtagaatcg gataaagatg ctcgtagcta ccgtgtggat cgcctgcaaa
    10561 aagtatcatg ttatttcctg tggcagttat tcaaattcgt gaatattata ttgcttaaac
    10621 tgtaatggga ttggttcttc atttaaaata gcttctacca cctctttggc ttgattgtag
    10681 cgatccaaat aacttggaga ttcaatttta atataaggaa tatggtgttt ttctagtagt
    10741 tttttcagta ggtgttgaaa ctgttgacgc tgttttttcg atcctaaact gcgtaaacca
    10801 tcggctaccc attttgtatt gttagaaagt aaaatcgtca catcaaaagg atattcgcgg
    10861 atcattgaat ccagaaacgg gtgagccttc ccttcatact gaatacagaa cgcttgagtt
    10921 gtgatgtaat cggtatcaat aatagccact ttatgagcat gtttcatggc ataatcaacg
    10981 tagcgttgat gtccaagcac catttgcgga tagtcggaat attgcattgc ttgttcattg
    11041 ccaccaagtt gctcaaacac aaactcacgc ccatattccc aagcagaggt agtgttaaat
    11101 acattcgcta atttgctcac caacactgat ttaccactgc tttcaccacc taaaatcgca
    11161 atagttttaa caaagaaagg acgaacttct ttcggaataa agcgccagta ttgaaaagga
    11221 ttattacgaa tttgtgttgc tgaaatatta aaggattcac gagtaggatc gacaagttga
    11281 acttctagat tcaaatattt ttcataagat tctttatctt gaatttcact actaaaaaca
    11341 atagtaggat taatgctttt ttcttcaaat agcgctttaa cacgaccaga ccaatcttcc
    11401 cagccatttg gatagctcgg aataccgtct tcaactaaat ggtgaataaa gatctgtttt
    11461 tgttggtatt taaaaatttg ctgtacccag cgtagccgat cttcatttgt cggcattcgt
    11521 ttcattttac tttcctggaa aagacgtaaa tcccgttcag aatcggtaca aacaataaca
    11581 tgtaaaacat ctactttact aaatgcttca tagatcatat taatatgacc tgtgtgaata
    11641 ggatagaatt tgccaaaaat aaccccggaa cgctggttgc tatcttctac gatatctaaa
    11701 acggaatgca aggctttaag cttattggcg cttggatttt tgatttttcc gcttactaac
    11761 tgattaaaat aagctcgggt taccccagct tgttcacaaa tatcattcac tttgagttta
    11821 agctgtttgc gcttttgttg aagataagca aaatctgtca taatgattcc ctcgattttt
    11881 tgat
//
LOCUS       DANLUQ010000068        11763 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.068, whole genome shotgun sequence.
ACCESSION   DANLUQ010000068 DANLUQ010000000
VERSION     DANLUQ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11763
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.068"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(100..2061)
                     /gene="recD"
                     /locus_tag="PXB27_001433"
     CDS             complement(100..2061)
                     /gene="recD"
                     /locus_tag="PXB27_001433"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HDL5346212.1"
                     /translation="MLNLLLELKQEKAISDLNYQFARFIDNKQKPYGYSPLEQDLAVL
                     AAALVSYQVGLGHTALRLENLSLSSLFELNSKPQTYHLLEQILQKIEQIPPLEWQSVL
                     KNHIAFSYSPETIKPMLFQNSLLYFYRYWQAEHRIAGYLQQAVDFSEKFANTELNQSI
                     LNQLFTGKAQTIDWQKIAVATALEKQFSLISGGPGTGKTTTVVKLLLGLQLKQQALQK
                     PYLQIALAAPTGKAAARMKESLESKLAEEKGLSEELKRSIPTEAVTIHRLLGARPLTE
                     KTKYHTKNPWHYDLVVLDEASMIDLSMMEKVVQALKPNSRLIMLGDKDQLASVEAGSI
                     MGELGSFIDNGYSQSHCDYLKAVTDYQIEAGNALPIGDCLSHLKHSYRFGEKAWIGEL
                     ANAVNDQKINLSWEIFDKYKNSDKLENFCYPETKETTDKSSWIEKSVQMVVDKAVELY
                     RDYLQAVQKREKNPDSVSVKAIFEKFQKVRILSALRVSELGVERLNMAIAEGLRKASL
                     VKFNHSRDRYIGKPILMTENMPINSIASGDVGIILPDENGESRVYFDSLQENREHHSL
                     PLGRVSNYEAAYIMTVHKSQGSEFEHTVLVLPHSISPVLTKELIYTAITRAKDKFTLF
                     GSEKVWKYSVGAKTERQSGLKEQIRGKFS"
     gene            2191..3315
                     /gene="alr"
                     /locus_tag="PXB27_001434"
     CDS             2191..3315
                     /gene="alr"
                     /locus_tag="PXB27_001434"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDL5346213.1"
                     /translation="MKPATATIDSQALRHNIRLIKSFAPQQKLLAMIKANAYGQGLLP
                     AAGTLADMVDGFGVARLREALEIQETGYTGKIVLVEGFFDREELLKTLSRKFDTVIHN
                     LEQLELLEQVAKEWQAEQQKGFWTRKAKIYFPINIWLKIDTGMHRLGIHPEQVDHFVE
                     RLKNCALVNSISFVSHFSRADELDCGYTEKQIAVFEQATQKYQDHDRSISASSGILYW
                     KQAHYDWVRPGIIMHGISPHYTPITDLGFKPVMTLSSSLIAVRTHKAGEPVGYGGAWV
                     SPKDTKLGVTAMGYGDGYPRNAPEGTPVLINGRIVPIVGRVSMDMLTVDLGAESQDKV
                     GDKAIFWGEQLLIEDVAKHIGVISYELITKLTPRVIFEYK"
     gene            complement(3378..4019)
                     /gene="sodA"
                     /locus_tag="PXB27_001435"
     CDS             complement(3378..4019)
                     /gene="sodA"
                     /locus_tag="PXB27_001435"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005708524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HDL5346214.1"
                     /translation="MAYTLPELGYAYDALEPHFDAKTMEIHHSKHHQAYINNANAALE
                     AHPELLEKCPGALIKDLSQVPAEKRIAVRNNVGGHVNHTLFWKGLKTGTTLQGALKEA
                     IERDFGSVEAFQSEFEKAATTRVGSGWAWLVLEEGKLAVVSTANQDNPLMGKEVAGVS
                     GYPILVLDVWEHAYYLNYQNRRPDFIKAFWNVVNWDEAARRFEEKVATCGCAK"
     gene            4463..6919
                     /gene="thrA"
                     /locus_tag="PXB27_001436"
     CDS             4463..6919
                     /gene="thrA"
                     /locus_tag="PXB27_001436"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HDL5346215.1"
                     /translation="MRVLKFGGTSLANPERFMQAAEIIEKAHLADQAAGVLSAPAKIT
                     NHLVAIVDKAIAGESFQSNLSEATEIFHNIINGLYATNNHFAKEALLALVEAELNQIQ
                     QVAAEAAQTKSLSDKIAATIHCRGEKLSIAMMQAWFEAKGYEVTRIDPVEKLLAHGSY
                     LESSVDIAESTKRVDAGSIPKKNIVLMAGFTAGNEQGELVLLGRNGSDYSAACLAACL
                     KADVCEIWTDVDGVYTCDPRLVPDAVCLETMSYQEAMELSYFGAKVIHPRTIGPLVPL
                     NIPCLIKNTGNPDGKGTLIDGNVANDNLKVKGITNLDNVAMFNVSGSGMQGMVGMAAS
                     VFSTMSKAGISVILITQSSSEYSISFCVPAKSAEKALAALNTEFAQELSENLLDPIDI
                     IKDLSIVSVVGDGMRTAKGIAARFFSALAQANISIVAIAQGSSERSISAVVPLNKAIE
                     AVKATHKSLFNSKRVVDMFLVGVGGVGSELIEQVKQQKEFLAKKDIEIRVCALANSDK
                     MLLNENGLNLDNWKEDLEKAIQPSDFDVLLSFIKFHHVVNPVFVDCTSAQSVANLYAR
                     ALKEGFHVVTPNKKANTSGYDYYLEMRENARQSQHKFLYETNVGAGLPVIENLQNLLA
                     AGDEVEKFEGILSGSLSFIFGKLEEGLTLSQATLIAKEKGFTEPDPRDDLSGADVARK
                     LLILVRETGLPLEFEDIEIEGVLPKGFSEGMSRDEFLQALPSLDEEFSKRVEAAKAEG
                     KVLRYVGSITGDKCRVAIEAVDENHPLYKVKDGENALAFLTRYYNPIPLLLRGYGAGN
                     SVTAAGIFADILRTLQGGVN"
     gene            6919..7866
                     /gene="thrB"
                     /locus_tag="PXB27_001437"
     CDS             6919..7866
                     /gene="thrB"
                     /locus_tag="PXB27_001437"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HDL5346216.1"
                     /translation="MALRIYAPASSANISVGFDSLGAAISPIDGSLLGDVVQIEESEQ
                     QFELESAGYFVRKLPKEPQKNIVYQAYVLFSERLKLRNGSVKNLRLTLEKNMPIGSGL
                     GSSACSIVAALVALNKFHDEPFSKMELLEMMGELEGRISGSIHYDNVAPCYLGGLQLM
                     TQSLGNICQPLPFFDEWYWVLAYPGVEVSTAEARAILPKSYTRQDMIQQARYLGSFVH
                     ACHTHQDILAATMMKDLIAEPYREDLLPNFPAVRQGCKDLGALAVGISGSGPTMFAIA
                     PDLEHAQRLVSYLEKEYLQNNEGFIHICKVDNQGARELK"
     gene            complement(7981..9357)
                     /gene="cysS"
                     /locus_tag="PXB27_001438"
     CDS             complement(7981..9357)
                     /gene="cysS"
                     /locus_tag="PXB27_001438"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDL5346217.1"
                     /translation="MLKIYNTLKREKEEFKPINPNQVGMYVCGVTVYDLCHFGHGRTF
                     VSFDVIARYLRYSGYNLRYVRNITDVDDKIIKRALENNETCDELVDRMIAEMHKDFDA
                     LNILRPDVEPRATKHIPEIIAMVEKLIHNGHAYVAADGDVMFDVESFQKYGALSRQNL
                     EQLQAGARVEIKSIKKNPMDFVLWKMSKAGEPSWESPWGNGRPGWHIECSAMNSKELG
                     EHFDIHGGGSDLMFPHHENEIAQSCCAHGGDYVNYWLHTGMLTIDKEKMSKSLGNFFS
                     IRHMLGLYDAESLRYFFLTAHYRSLLDYSIDNLDLARSALERLYTALRGCDVSGEAIG
                     EHYVEAFKTAMDDDFNTPGALAVLFEIARELNKLKTEDMAKANGLAVRLKELAGVLGL
                     LEQDPDAFLKGDANDDETAEIEALIKQRNEAKAAKNWAVADEVRDKLKAINIVLEDTP
                     NGTIWRKA"
     gene            9513..10022
                     /locus_tag="PXB27_001439"
     CDS             9513..10022
                     /locus_tag="PXB27_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL5346218.1"
                     /translation="MITLHTNFGDIKIELNFEKAPITAKNFEDYAKEGFYNGTIFHRV
                     IDGFMIQGGGMTAGMAEKPTKDPIQNEANNGLSNKRGTIAMARTSDPHSASSQFFINV
                     ADNTFLDYRSKEMFGRSVVQDWGYAVFGEVVEGMDVVDKIKGVKTGNKGFHQDVPVED
                     VVIESVSVA"
     gene            complement(10114..11043)
                     /locus_tag="PXB27_001440"
     CDS             complement(10114..11043)
                     /locus_tag="PXB27_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDL5346219.1"
                     /translation="MEIALLLADKILQLTLMVLLGYLIVKTKLLKSEHSYPISVIGLY
                     IISPAMLITAFQVDYTTEILNGLYLAFLISVLLNGLLILLGWLLKRALKLDNIEQATA
                     IYSNSGNLIIPIVASLFGNEWVIYSTAFIVVQNVLFWSHLRLLICGKGNVPFRKMIFN
                     INIIAIVIGMMLFLLHIKLPATLANTLSTLGHMLGPLAMLVAGMLIAAIPFKEIITDK
                     RIYLVAFLRLIFIPLILLAVIKLGGFASWIENGKTVVMITFLATISPSAATATQMAVA
                     YGQDAKKASAIYGVTTLLCVFTMPLIIWLYQFL"
     gene            complement(11046..11723)
                     /gene="fdhD"
                     /locus_tag="PXB27_001441"
     CDS             complement(11046..11723)
                     /gene="fdhD"
                     /locus_tag="PXB27_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012621653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HDL5346220.1"
                     /translation="MMATPANLEDFALGFSLAEGILNAPSELYGLDVIEQENGVEIQL
                     EISSRQFVALKDKRRSMAGRTGCGICGVEQLEQVNQSCKIVKKNNRLQNVNPLILEDC
                     LLQLENAQFLSKETGASHAAAFFTPDGKLLAIREDVGRHVALDKLIGWYAKADFPIGF
                     VFVTSRASFEMVQKCLAVGIEMLCAISATTEMAVNMARENQFTLLAFARKGKATIYAG
                     EERLALN"
BASE COUNT         3421 a         2512 c         2406 g         3424 t
ORIGIN      
        1 gcgtattata gggaaaaaca aaaacaacgc aagcggtttt tgtaaaaaat ttgaaaaaaa
       61 tgaccgcttg cgtattttta cggcaaaacg agtattaatt tagctaaact tgcctcttat
      121 ttgttctttt agcccacttt gtctttcggt tttggcacca acactgtatt tccaaacttt
      181 ttcgctacca aataaggtaa atttatcttt tgcccttgtg attgcggtgt aaatcaactc
      241 tttcgtcagc accggggaaa tcgaatgggg caacaccaat accgtatgtt caaattcaga
      301 accttgcgat ttatgcacag tcataatata agctgcttca taatttgaaa ccctacctag
      361 aggcaaacta tggtgttctc tattttcctg taaggaatca aaataaactc ggctttctcc
      421 attttcgtcc ggcaaaatga tgccgacatc accgctggct atgctattta tcggcatatt
      481 ttctgtcatt aaaatcggtt ttccgatata gcggtctcgg ctatgattaa atttcactaa
      541 gctagctttc cgcaagcctt ccgcaatcgc catattaaga cgttcaaccc ccagttcact
      601 tacccttaag gctgataaaa ttcggacttt ttgaaatttc tcaaaaatcg ctttcacact
      661 cactgaatct ggatttttct cccgtttttg aaccgcttgt aaataatcac gataaagttc
      721 aaccgcttta tccaccacca tttgcacact tttttcaatc cagcttgatt tatccgttgt
      781 ctctttcgtt tccggataac agaaattttc taacttatca ctgtttttat atttgtcgaa
      841 aatctcccac gaaaggttaa ttttttgatc atttactgca tttgcaagct ctccaatcca
      901 ggctttttcg ccaaatcgat agctatgttt taagtgagat aaacaatcac caatcggcaa
      961 cgcattaccg gcttcaattt ggtagtcggt aacggctttt aagtaatcgc aatgggattg
     1021 gctataacca ttatcaataa aagagccaag ttcccccata attgaaccag cttccacaga
     1081 agcaagctga tctttatctc ccagcattat caagcgagag tttggtttga gcgcttgaac
     1141 aactttctcc atcattgata aatcaatcat tgaagcctca tctaatacga ccaaatcata
     1201 gtgccacgga tttttggtat gatatttggt tttctcagtt aatggtctcg caccaagcaa
     1261 gcgatgaata gtcactgctt cagttggaat agagcgtttt aactcctcag ataacccctt
     1321 ttcttccgcc aacttgcttt ccagcgactc tttcattctt gctgctgctt ttccggttgg
     1381 tgcagccaga gcgatttgca aatatggttt ctgtagtgct tgctgtttta attgcagccc
     1441 gagtaataat tttactaccg tagttgtttt tccggttccc ggcccacctg aaattaagct
     1501 aaactgcttt tctaatgctg ttgcaacggc aatcttttgc cagtcgatag tttgagcttt
     1561 cccagtgaat agttggttga gaatgctttg gtttaattcg gtatttgcaa atttttccga
     1621 aaaatcaacc gcttgttgta aataaccggc aatgcgatgc tccgcttgcc agtagcgata
     1681 aaaatagagc aagctgtttt ggaataacat cggtttaatt gtttcgggag aatagctaaa
     1741 agcgatatga tttttcagca cactctgcca ttcaagcggt ggaatttgct caattttttg
     1801 caaaatttgt tcgagcaaat gataagtttg tggcttgcta tttaattcaa aaagagaaga
     1861 aagactgaga ttttccaaac gtaatgccgt atgcccaagt cccacttggt aagaaaccaa
     1921 cgcagcggct aacacagcta aatcttgctc caaaggcgaa tacccatagg gtttctgctt
     1981 attatcaata aaacgggcaa attgataatt taagtcagaa atcgcctttt cttgtttaag
     2041 ttccaataat aaattcagca taatcaaacc ttaaatcgac cacctaacat taagtgataa
     2101 tactaatctt ttttgcatta ttttctaagg gattttttat tttaggcgta taatttagcc
     2161 tttcttgtga ttttaactat gtagataagt atgaaaccag caacagcaac cattgatagt
     2221 caagccctcc gccataatat tcgcctgatc aaatctttcg ctccacagca aaaattattg
     2281 gcgatgatca aagccaatgc ttacgggcaa ggcttattgc cggctgccgg cacattagca
     2341 gatatggttg atggctttgg agttgcccgt ttgcgtgaag ccttagaaat tcaagaaacc
     2401 ggatacacag gcaaaattgt gttagttgaa ggtttttttg accgagaaga gctactcaaa
     2461 accctttccc gcaagtttga taccgtcatt cataacctcg aacaactgga attactcgag
     2521 caagtagcaa aagaatggca ggcagagcaa caaaaaggct tttggactcg taaagcgaaa
     2581 atctacttcc cgattaacat ttggctaaaa atcgacaccg gaatgcaccg cttaggcatt
     2641 cacccggagc aagtcgatca ctttgttgaa cgtttgaaaa attgtgcctt ggtcaattca
     2701 atcagctttg tgagtcattt tagccgtgcc gatgaactcg attgtggcta taccgaaaaa
     2761 caaattgcgg tgtttgagca agctacgcaa aaatatcagg atcacgaccg cagtatttcc
     2821 gcctcaagcg gtattcttta ttggaaacag gctcattatg attgggttcg cccgggtatt
     2881 attatgcacg gcatttctcc ccactatacg ccgattaccg atttaggttt taagccggta
     2941 atgacgctct cctcttccct gatcgctgtg cgtacccata aagccggcga gcctgtaggc
     3001 tatggtggtg cttgggtaag ccctaaagat accaaactcg gcgtgactgc aatgggctac
     3061 ggcgatggct accctcgcaa tgctcctgaa ggtacccctg tgttgattaa cggtagaata
     3121 gtgccgattg tcggtcgagt ttcgatggat atgctgacgg tcgatttagg tgcagagagc
     3181 caagataaag tgggcgataa agccattttt tggggagagc aactattaat tgaagatgtg
     3241 gcgaaacaca ttggcgtgat tagctatgag ctgattacta aactcactcc tcgtgtgatt
     3301 tttgaatata aatagacaac aagcggtaaa attttcaaca aattttaccg cttgtatctt
     3361 attcaagatc gaaaatatta tttcgcacaa ccgcaggttg ctactttttc ttcaaaacgg
     3421 cgagcggctt cgtcccagtt tacaacgttc cagaatgctt taatgaaatc cggacggcgg
     3481 ttttggtagt ttaagtagta agcgtgttcc caaacgtcta agactaaaat cggatagcct
     3541 gaaacgcctg ccacttcttt acccattagt gggttgtcct ggttagcggt tgaaacaacg
     3601 gctaatttac cctcttctaa tactaaccac gcccagcctg aaccaacacg agtagtcgct
     3661 gctttttcaa attctgattg gaaagcttca actgaaccga aatcacgctc aatcgcttct
     3721 tttaatgcac cttgtaaggt agtgcctgtt tttaaacctt tccagaataa agtgtggttt
     3781 acatgaccac ctacgttgtt acgcactgca atacgttttt ctgcaggcac ttggctcaaa
     3841 tctttgatta atgcacccgg acatttttct aataattcag ggtgagcttc taatgctgca
     3901 tttgcattgt tgatgtaagc ttggtgatgt ttagaatggt ggatttccat tgttttagca
     3961 tcgaaatgtg gctctaacgc atcataagcg tagcctaatt ctggtaatgt atatgccata
     4021 gttaattcct ctcaaatagt aaataggtta atcagagtta aactccgagt acattttatc
     4081 aagaaacaca caaaaaatgc acattttttt gatctagaac aaaattattt cactatctaa
     4141 tttagaaagg ttcctaatca aattcttgct tttaattgtt ttatatagaa agacaaagaa
     4201 atttaaaaaa atgtggtgaa aaacagcgtt aagcccattg acaaaggcat tattttcacg
     4261 cattcttgaa ccattattca ctctcttgac cgagattccg ccaatgaatt gttttaatat
     4321 gatgacaacg attattacca ccattatgtc accaacatag tgcgggtttt aattggctga
     4381 aataatgaaa gaataaaccg aaaacccgct acaagcgggt ttttttgtat aaaaatttac
     4441 aaataattta tcaggagtaa ctatgcgagt tcttaaattt ggaggcactt ctcttgccaa
     4501 ccctgaacgc tttatgcaag cggcagagat tatcgagaaa gctcatttgg cagatcaagc
     4561 tgccggcgta ttatccgccc cagccaaaat taccaatcat cttgtcgcta ttgtagataa
     4621 agcgattgcc ggtgaatcat ttcaatctaa cctttctgaa gctaccgaga ttttccacaa
     4681 catcattaac ggcttatatg ctacaaataa tcactttgct aaagaagcac tgttagcctt
     4741 agtggaagcg gaattaaacc aaattcagca agtggctgca gaagcagctc aaactaaatc
     4801 gttgtcagat aaaattgctg ccactattca ctgccgtggt gaaaaactct ctattgcgat
     4861 gatgcaagcg tggttcgagg caaaaggcta tgaagtcact cgtatcgacc ctgttgaaaa
     4921 attattggct cacggcagct atttggaatc ttcggtcgat attgccgaat ccaccaagcg
     4981 agttgatgca ggttcaattc caaagaaaaa tatcgtgcta atggctggtt ttaccgcagg
     5041 taatgagcaa ggcgaactgg tgttattagg gcgtaacggt tcagattatt ctgccgcttg
     5101 tttggcagcg tgtttaaaag ccgatgtttg tgaaatctgg acggacgtag atggcgttta
     5161 cacctgcgac cctcgtttgg tgcctgatgc agtatgctta gaaacaatga gctatcaaga
     5221 ggcaatggag ctttcctact ttggggcgaa agtgatccac cctcgcacca tcgggccatt
     5281 agtgccgctc aatattcctt gtttaatcaa aaataccggc aatccggacg gaaaaggcac
     5341 attaattgat ggcaatgtgg caaacgataa tctcaaagta aaagggatta ccaaccttga
     5401 taacgtggcg atgttcaacg tttcaggttc aggaatgcag ggaatggtgg gcatggctgc
     5461 cagtgtgttc tcgacgatgt caaaagccgg tatttcggtg attttaatta cccaatcatc
     5521 atcagaatat agcatcagct tctgcgtgcc ggcaaaatcg gcagaaaaag cgttagcggc
     5581 tctaaatact gaatttgcac aagagttatc tgaaaactta cttgacccga ttgatattat
     5641 taaagattta tccattgttt cggtagtggg tgatggtatg cgtaccgcca aaggaattgc
     5701 ggctcgcttc ttctctgccc tagcccaagc caacatcagt attgtggcaa ttgcacaggg
     5761 ttcatctgag cgttccattt ctgctgtcgt gccgttaaac aaagcgattg aagcagtaaa
     5821 agcaactcac aaatcgctat ttaacagtaa acgtgtggtg gatatgtttt tagtgggtgt
     5881 aggcggtgtg ggtagcgaac tcatcgagca agtcaaacaa caaaaggagt ttttggcaaa
     5941 gaaggatatt gagatccgtg tgtgtgcttt agctaattca gataaaatgt tactcaacga
     6001 aaacggctta aatttagaca actggaaaga agatttagaa aaagcaattc agccgtcaga
     6061 ctttgatgta ttgctttctt tcatcaaatt ccaccacgtg gtaaacccag tatttgtaga
     6121 ttgcacttcg gcacaatcgg tggcgaacct gtacgcacga gccttaaaag agggcttcca
     6181 cgtggtaaca ccaaacaaaa aagccaatac atcaggttac gattactact tagaaatgcg
     6241 tgaaaacgcc cgccaaagcc agcacaaatt cctctacgaa actaatgtag gggcaggttt
     6301 accggtaatt gaaaacttac aaaacctatt agctgccggt gacgaagtag agaaatttga
     6361 agggatttta tccggctcgc tctctttcat tttcggcaaa ttagaagaag gtttaaccct
     6421 ttcgcaagca actttaatcg ccaaagaaaa aggctttacc gagccagatc cacgagatga
     6481 tttatccggt gcagatgttg ctcgtaaact cttaattctt gttcgtgaaa ccggcttacc
     6541 gcttgaattt gaagatattg aaattgaagg cgtattgcca aaaggcttct cggaaggaat
     6601 gagtcgtgat gagtttttac aagctctacc gtctttagac gaagaattca gcaagcgtgt
     6661 agaagctgcc aaggctgaag gcaaagtgtt gcgttatgtg ggctcgatca caggtgataa
     6721 atgccgagtt gccattgaag ccgtggacga aaaccaccca ctttacaaag ttaaagacgg
     6781 cgaaaatgcc cttgcttttc tcacacgtta ctacaaccct attccattat tattgcgtgg
     6841 gtatggtgcg ggcaatagcg taaccgctgc cggtatcttt gcagatattc tgcgtacttt
     6901 acaaggagga gtgaactaat ggcgttacga atttatgccc cagcttcaag tgcaaatatt
     6961 agtgtgggtt ttgactcatt aggcgctgca atctccccga ttgacggctc tttgctcggc
     7021 gatgtagttc aaattgaaga gagtgaacaa caatttgagc tagaaagtgc aggttatttt
     7081 gtacgtaaat tgcccaaaga accacaaaaa aacattgtct atcaagccta tgtgctattt
     7141 agcgaacgct tgaaattacg taacggcagt gtaaaaaatt tacgcttaac acttgaaaaa
     7201 aatatgccga ttggctcagg gctaggctca agtgcctgct cgattgttgc agcattagtg
     7261 gcactcaata aattccacga tgaaccgttc tccaaaatgg aattactgga aatgatgggc
     7321 gaattggaag gcagaatttc aggctcaatc cactacgaca acgttgcccc ttgctattta
     7381 ggtggcctgc aattaatgac acaatcgctc ggcaatattt gccaacctct gccattcttt
     7441 gatgaatggt attgggtgct tgcctacccg ggcgtggaag tatcaaccgc cgaagcccgt
     7501 gctattttgc cgaaaagcta tactcgtcaa gatatgattc agcaagcccg ctacttagga
     7561 tctttcgtcc atgcttgcca tacacatcaa gatatcttgg ctgctacaat gatgaaagac
     7621 ctaattgctg agccttaccg tgaagattta ctgccgaact ttccggctgt tcgtcaaggc
     7681 tgtaaagact taggtgcgtt agcggttggt atttcaggtt ccggtccgac aatgtttgcg
     7741 atagcccctg atctcgaaca cgctcaacgt ttagtgagct atttagagaa agaatatctg
     7801 caaaacaacg aaggctttat tcatatctgt aaagtcgata atcaaggagc aagagagctg
     7861 aaatagccct ctcgaacaac catatcacta aataacttaa aggactgact ccaataccac
     7921 ttggagcagc cctttttgat taattttttg aaaagtctat cacttataaa caatcgaagg
     7981 ctatgcctta cgccaaattg tgccgtttgg ggtgtcttcg aggacaatat ttatcgcttt
     8041 gagcttatct cgcacttcat cggcaaccgc ccagttttta gccgctttgg cttcgttgcg
     8101 ttgtttgatt aaggcttcga tttctgccgt ttcatcgtca ttcgcatcac cttttaaaaa
     8161 cgcatcgggg tcttgttcca gtaagcctag cacgccggct aactctttta aacgaacggc
     8221 taaaccgttg gcttttgcca tatcttcggt tttgagtttg ttcaactcac gggcaatttc
     8281 aaataacacc gctaatgccc ccggggtgtt gaaatcatcg tccattgcgg ttttaaaggc
     8341 ttctacatag tgttctccga tggcttcgcc cgacacatca caaccacgca gagcggtata
     8401 taaacgctca agagccgaac gggctaaatc aaggttgtca atgctgtaat ctaataacga
     8461 acggtaatga gcggttaaga agaaataacg taacgattcg gcatcgtata agcctaacat
     8521 atggcgaatg ctgaagaaat tgcccaaaga tttcgacatt ttttctttat caatggtaag
     8581 cattccagtg tgtaaccaat aattgacata atcgccaccg tgagcacagc aagattgtgc
     8641 aatttcgttt tcgtgatgcg ggaacattaa atccgagcca ccgccgtgaa tatcaaaatg
     8701 ttcgcccaac tctttgctat tcattgccga acattcaata tgccagcccg gtctgccatt
     8761 gccccacggg ctttcccagc ttggctcacc ggctttcgac attttccaca agacaaaatc
     8821 catcgggttt ttcttgatgg atttaatttc aacccttgca ccggcttgta gttgctctaa
     8881 attttggcgg gataatgcac catatttctg gaacgattcc acatcaaaca tcacatcgcc
     8941 atcggctgcg acataagcgt gtccgttgtg aatcagtttt tctaccatcg caataatttc
     9001 aggaatatgc ttggtggctc gtggctcaac gtccgggcga agaatgttta acgcatcaaa
     9061 atctttgtgc atttcagcaa tcattctatc cactaattca tcgcaagttt cgttattttc
     9121 taacgcacgt ttgatgattt tgtcgtccac atcggtaatg ttacgcacat agcgtaaatt
     9181 gtagcctgaa taacgtaaat aacgggcaat cacatcgaaa gagacaaaag tgcgaccgtg
     9241 cccaaaatga caaagatcgt aaaccgtgac gccacagacg tacattccca cctgatttgg
     9301 gttaatcggt ttaaattctt ctttttcacg ttttaaggtg ttataaattt tgagcataaa
     9361 gtcctcttgt gtaacattca gtaacaaatg gggctaagta tagcgaataa gcggtctgat
     9421 tttgagattt ttttgcaatt tggcgtaaac tttttaataa tagcgagcga aatttatttt
     9481 ccattccatt tttatttata aaggaacaaa gaatgattac attacatacc aatttcggtg
     9541 acattaaaat cgaattaaac tttgaaaaag ccccgattac cgctaaaaac tttgaagatt
     9601 acgctaaaga agggttttat aacggcacga ttttccaccg tgtgattgac ggctttatga
     9661 ttcaaggtgg cggaatgacg gcaggtatgg ctgaaaaacc gacaaaagat ccgattcaaa
     9721 acgaagcgaa caacggttta agcaacaagc gtggcacaat cgcaatggct cgcacttctg
     9781 atccgcattc tgcttcgtca cagtttttca ttaatgtggc ggataatact ttcttagatt
     9841 atcgctcaaa agagatgttc ggcagaagtg tagtgcaaga ttggggctat gcagtatttg
     9901 gcgaagtggt tgaaggtatg gacgtggtcg ataaaatcaa aggcgtaaaa accggcaata
     9961 aaggcttcca ccaagacgtg ccggttgaag atgtggtgat tgagtctgta agcgttgcct
    10021 aattccgcaa attagcctac aagcggttga tttttcacaa ttttttgcat ttctcagcgt
    10081 tgcaaaaaag tagcagaaat cgactgcttg tttttataga aattgataca gccaaataat
    10141 cagcggcatt gtaaacacac aaagtaaagt ggttacgcca taaatcgcac tggctttttt
    10201 ggcatcttgc ccgtaagcca ccgccatttg ggtcgccgtt gcagcagaag ggctgatggt
    10261 ggctaagaat gtaatcatta ccaccgtttt tccattctct atccagctcg caaaaccgcc
    10321 gagtttaatc accgctaaca aaatcagcgg aataaaaatc aagcggagaa aagccaccaa
    10381 gtaaatgcgt ttatcggtaa taatctcttt aaatggaatt gctgcaatta acatccctgc
    10441 cacaagcatt gctaaaggcc ctaacatatg ccctaaagtt gagagcgtgt ttgcaagcgt
    10501 agcgggcagt ttgatatgta gcaaaaacaa catcatacca atcacaatcg caataatgtt
    10561 gatattaaag atcatctttc taaacggcac attgcctttg ccacaaatca gcagccgtaa
    10621 gtgagaccaa aacaacacat tttgcaccac aataaaagcc gttgaataaa tcacccattc
    10681 attgccgaac agggaggcaa caatcggaat aattaagttg ccggagttgg aataaatagc
    10741 ggtggcttgt tctatgttgt ctaatttcaa cgctcttttg agcagccaac caagcagaat
    10801 taacaagccg tttaataaga cggagattaa aaatgccaga tacaagccgt ttagtatttc
    10861 ggtggtgtaa tctacttgaa aagcagtaat cagcattgcc gggctgataa tgtaaagtcc
    10921 gataaccgaa atcggataac tgtgctccga tttgagtaat ttagttttaa cgatgaggta
    10981 gccgagcagc accatcaggg ttaattgtag aattttatcg gctaataaaa gggctatttc
    11041 cattatcaat taagagcgag tctctcttct cctgcataaa tggtcgcttt gcctttgcgg
    11101 gcaaaagcaa gtaaggtaaa ttgattttca cgagccatat tcactgccat ttcagtggtc
    11161 gctgagatgg cacacaacat ttcaatgcca acggctaagc atttctgcac catttcaaag
    11221 ctggcacgac tggttacaaa cacaaatcca atcggaaaat cagctttagc ataccaaccg
    11281 attaatttat ccaacgccac gtgtctgccc acatcttcac gaatggcaag cagttttcca
    11341 tccggggtaa aaaatgcagc ggcgtggctt gcaccggttt ctttcgataa gaattgagca
    11401 ttttctagct gtaataaaca atcctctaaa atcagtggat tgacgttctg caagcggtta
    11461 tttttcttaa caattttgca actttgatta acctgctcta attgctctac accgcaaata
    11521 ccgcaacctg ttctgcctgc cattgagcgg cgtttatctt ttaatgcaac aaattggcgg
    11581 ctcgatattt caagctgaat ttctactcca ttttcttgtt caattacatc taaaccatat
    11641 aattccgatg gggcatttaa aatcccttcc gccaatgaga atcctaaagc gaagtcctcc
    11701 aaattagccg gtgttgccat cattacagta tgagaaatac cgttatatac caaagcaacc
    11761 ggc
//
LOCUS       DANLUQ010000069        11757 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.069, whole genome shotgun sequence.
ACCESSION   DANLUQ010000069 DANLUQ010000000
VERSION     DANLUQ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11757
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.069"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            190..1698
                     /locus_tag="PXB27_001442"
     CDS             190..1698
                     /locus_tag="PXB27_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HDL5346221.1"
                     /translation="MSNQSSNAPQRETFSGRGAFIMAAIGSAVGLGNIWRFPYTTYEN
                     GGGAFILPYIIALLTAGLPLLFLDYSIGHKYRGGAPLAFRRFSSKFETFGWWQVLINV
                     IIGIYYAVVLGWAASYTYFSFSGAWGDKPVDFFIGEFLKMGDITQGVSFEFVGMVTGP
                     LIAVWLIAIAVLAMGIKKGISAASNLLMPQLIVMFLVLVGYSLTLPGAAKGLDALFTP
                     NWEKLADPSVWIAAYGQIFFSLSICFGIMITYASYLKKETDLTGSGLVVGFANSGFEL
                     LAGIGVFAALGFMATAAGQEVNEVAKGGIGLAFFAFPTIINQAPMGALVGVLFFGSLT
                     FAALTSFISVIEVIISAVQDKLNSGRVKATLLVGIPMMVVSVLLFGTTTGLPMLDVMD
                     KFVNQFGIVAVAFVSLIAIVTSEKLSTLEKHLNEVSSFKVGFIWRLCIVITSGVLAFM
                     LFSEIAKVYREGYEGYPSWFVNTFGWGMAIGLVVVAYLLSKMAWKNEPKGDE"
     gene            1698..1796
                     /locus_tag="PXB27_001443"
     CDS             1698..1796
                     /locus_tag="PXB27_001443"
                     /inference="COORDINATES: protein motif:HMM:NF028261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HDL5346222.1"
                     /translation="MSSAAIITMVVAMTIIWGGLLFAIKRLPKEEQ"
     gene            complement(1844..2107)
                     /locus_tag="PXB27_001444"
     CDS             complement(1844..2107)
                     /locus_tag="PXB27_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrxA family glutaredoxin"
                     /protein_id="HDL5346223.1"
                     /translation="MFVEIYGRLSCPYCVRAKTLAEKMKQELSDFDFKFIDMIAENIS
                     KQDLEPRVGKPVATVPQIFVDNNHVGGCTDFQAFVEEKFGITV"
     gene            2267..3601
                     /gene="srmB"
                     /locus_tag="PXB27_001445"
     CDS             2267..3601
                     /gene="srmB"
                     /locus_tag="PXB27_001445"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HDL5346224.1"
                     /translation="MTQLMTFEELDLSPQLLKALAHKGYNRPTAIQQATIPAALEGRD
                     LLGSAPTGTGKTAAFLLPAIQHLLDYPRRKPGAPRILILTPTRELAMQVAEQAQALAK
                     FTQLSIATITGGVAYQNHGEVFNKNQDIVVATPGRLMQYIKEENFDCRAVEVLIFDEA
                     DRMLQMGFGQDAEKIAAETRWRKHTWLFSATLEGELLEDFAERLLNDPIKVDAEPSRR
                     ERKKIQQWYYHADNVEHKTKLLARIISEFSVERAIVFVRRREDVRELSETLRKRGIRS
                     TYLEGEMAQTQRNQAINRLKEGVVNVLVATDVAARGIDIDDIDFVLNYDLPYSADTYL
                     HRIGRTARAGKKGSAISLVEAHDYKLLGKIKRYTEEVLKPRIIEGLEPRTKAPKDGEL
                     NVTSKKEKARIKKKKEAKKEAAKPKVKVRHKDTKNIGKRRKPSSESAAVKQA"
     gene            complement(3670..3963)
                     /locus_tag="PXB27_001446"
     CDS             complement(3670..3963)
                     /locus_tag="PXB27_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HDL5346225.1"
                     /translation="MSKTMQQKAAEAHNMCRLKGDSMLDNADRQIAFEAVYESEELAQ
                     QAVEFFAQKAKSVETEPCEIQSEITQIAEGFLMKMWITFSCQAEVILFQMAVR"
     gene            complement(3965..4627)
                     /locus_tag="PXB27_001447"
     CDS             complement(3965..4627)
                     /locus_tag="PXB27_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDL5346226.1"
                     /translation="MPFPDEISELKGYFDGTNPVSAYPAQGLNVPFYILGSSPESAYL
                     AAERGLPYAFASHFAPRFMDDAIRIYRSHFKPSDVLDKPYVILGVNAIVAETDEEATQ
                     LATTQTQFFLNVVTNAQQNLQPPLESDDKVWENFVKAEIVPHFGPVAFEQNALYRQEK
                     TVVRQMTECSLIGSPQSVEQQLKALKARVEIDEIMAVNYIFDEQKQHQSYTWLKQITD
                     KV"
     gene            complement(4611..5063)
                     /locus_tag="PXB27_001448"
     CDS             complement(4611..5063)
                     /locus_tag="PXB27_001448"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MsnO8 family LLM class oxidoreductase"
                     /protein_id="HDL5346227.1"
                     /translation="MVLQVLNSKAIQPFLYANSENKMTNLSILNLVPIREGQTAKQAV
                     DSMVKLAKHAENIGIERYWIAEHHNMKNLASSATQLLIQHALSHTEKLRVGSGGVMLP
                     NHSPYIVAEQYGTLETLYPNRVELGLGRAPGTDMRTASALRPEFAFPR"
     gene            complement(5083..5721)
                     /locus_tag="PXB27_001449"
     CDS             complement(5083..5721)
                     /locus_tag="PXB27_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDL5346228.1"
                     /translation="MKKFALKTVLVALSALFAFNATAVAADPTAGKEYIEVRKAPSAQ
                     KEVVEFFSFYCPHCYDFELNYKIPSQIKAKLPADSKLVQYHVNFLGRQSENLTRAWAL
                     AMALGAEDKVKTALFEAAQKDAFKSMDDIRAVFIANSISAEQFDSGINSFAVNGLVNK
                     QVQLAEDFQIRGVPAFFVNGQYQLNLEGFSDSSSTNDFIQRYIDAVVFLSKK"
     gene            complement(5765..6034)
                     /locus_tag="PXB27_001450"
     CDS             complement(5765..6034)
                     /locus_tag="PXB27_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HDL5346229.1"
                     /translation="MKCKRLEELLELLGEHWRKNPDLHLIDILQQLSVEVSEPDNFHA
                     LRDEVLIYQLKMRNADKFEPIPGIKKDYEDDFKTALLRARGILND"
     gene            6126..7235
                     /gene="mobA"
                     /locus_tag="PXB27_001451"
     CDS             6126..7235
                     /gene="mobA"
                     /locus_tag="PXB27_001451"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HDL5346230.1"
                     /translation="MEEKISAVILAGGLARRMNGAEKGLQLLDQKPLISHLLARLSPQ
                     IEDIWLNINRSVEQYQQLYPELPYYQDSLPDFQGPLSGMLSGFEQIESDYLLFVPCDT
                     PFIPTQLLHKLHTALRINQAQIAYAHDGDRPHPTVALVHRSVAENLRAYLASGERRLL
                     WFFQTQKSVAVDFSAYKAAFRNFNCLEDLTAFNPYPIKTLAITGYSGTGKTTLLEKLI
                     PKLTACGIRVGLIKHSHHNVDIDKKGKDSYRLREAGANPTMVVCDERWALMTETKQAA
                     ETKQAVEFSQLIAKFDPETVDLVLVEGFKHETMPKIQLHRKEIEKPLPALDQWTIATA
                     TDYPLERENRLDINDVEQLADFIRQWLEASKEPAN"
     gene            complement(7290..>11757)
                     /locus_tag="PXB27_001452"
     CDS             complement(7290..>11757)
                     /locus_tag="PXB27_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDL5346231.1"
                     /translation="AFAVSLSATASVFRDDIDLQYYRDFAENKGKFTVGASNIEIKDK
                     SGNTLDALFPNVPMPDFSAANRNLGIATLVAPQYLVSVAHNTQYNTVEFGAPGTNADA
                     HHYTYKVVDRNDYGIVEGGQHQDYQVPRLNKLVTEVAPATVTDLGNNASAYQDSSRFT
                     HFARLGSGRQIVKNTEHKDNQISTSYQYLTGGVHLPIAVHNSDYWLDFRGNALNNSPY
                     GALTAFGTRGDSGSGVYGYDQKTKRWLLLATYTFGTPANNYYNRAGIIRQDYHDKQFA
                     EDIAGTLTNAQQNAVFEWSAQGKDSSIGNKGKNLTVSLADRSIKDNSASPLGYGGTPQ
                     LQLPQDNTGKTLNIEGQDSTIVLKTDIDQGAGALNFNANATVRPENDQSWVGAGIVVA
                     KGKQVNWQVKNPQGDRLSKLGEGTLHINGKGENLGDISVGQGTVILTQQAGENGKKSA
                     FNQVGIVSGRSTVVLNSADQVDPNKIYFGFRGGRLDLNGNNIAFNRIQNSDDGARIVN
                     NHLQKAATLTINGPKPPEATDLKWGTWKENSADIYEYINPHANNRTDYFTLKGNPNQY
                     MPTNGASNAHWTFLSSNKDAAVKQVLAQKGLEHRYNSFNGFIGETDNTQHNGRLNVVY
                     DPKASTPPATASEVTWGKGLVAGADIYLFTNPKTKIREYFALKGDPKQPVPRGGLSSE
                     HWEFLAADRNQAINKVLARKNAPIEQEKLNSIYTFSGGLNLNGDLTVKGGKVLLSGRP
                     TPHAYDAINKQDVVYADDWQNRQFKADNMQLNQYAQLYVGRNVSNLQANLSANDHAQL
                     HLGFINEQTPSCYYSEYTGKTSCDTQAVVSNEIFATLPTTQINGDVSLAEQSQLHIGK
                     ANLIGTIQAAATTSIRLANQASWTNTGDSRTGNLVAENGSTINLNEKFVTGEIPTRFN
                     TLIIDGNFQGNAKINYLTDIAAGKGDHLQVNGLAEGTFTLALRNSGKEAEVVSPLSLL
                     TLTHQAQADKAKVSLENGYVDLGAYRYVLANRSNGYRLYNPLKDAKDRNQSIATAKAE
                     LDHAIAEADKQKQEISRLNAEAEKERQAEKNAKQAAANAQSQLSQANSELTRLQQYAD
                     YYRRYYPTYYRQIQGQITVAKQKVTQASAALTTAENNAKASAAQIANVEQAVETAQNI
                     AKQVEAKLATLRITAGNSEAILNAEALKLCQENGANCEHLQAHADTDSSNIQQSDWVS
                     QYANTALSELSAQANSALQIGQDLDRQLFAKHDKFHVWSSIEHQKTEHQSDLYRPYEQ
                     QTNLTQLGIELPLANGINAGVMLSRNHANAEFDEGVNGKSNLLMASLYGKWHSENGTF
                     VSLDGSYGKAKNRIDLFGENRFNRHIMAIGANLGHNFDLAGVQVQPAVGTRYYRFSAQ
                     NYKLGEVEVRSPKAHFMAYQAGVKVSKAFDLSSWKVEPSLAAHYVDASSKRLSVAVND
                     NTFSQRFGRYLKTEVGVGVTKGQWQLSSHLGLLKGNEIGKQHFAGFKLGYSW"
BASE COUNT         3082 a         2454 c         2644 g         3577 t
ORIGIN      
        1 tttttgtttg aatttttgtt tttatttgga gttttatatt gaaaattgag cgttttttat
       61 ctgtttttct atatttttct actttacatt caacctgaaa ttgggcatat tccgcactta
      121 aagtgtgatt aatttacatt taactcaatt cttttttata taaaacttta tcagtttgag
      181 gtatttaccg tgtctaatca atcatcaaac gcaccgcaaa gagaaacgtt ctccggacgt
      241 ggtgcattta ttatggcggc tattggttcc gctgtcggct tagggaacat ctggcgtttc
      301 ccttatacta cttatgaaaa cggcggtggg gcttttattc tgccgtatat cattgccctt
      361 ttgaccgcag gtttaccgct gctgttctta gattattcta tcgggcataa atatcgtggt
      421 ggtgcaccgc tcgctttccg ccgtttcagc tcgaaatttg aaaccttcgg ttggtggcaa
      481 gtgctgatca acgtgatcat cgggatttat tatgcggtgg tactcggctg ggcagcaagt
      541 tacacctact tctcattcag cggagcgtgg ggcgataaac cggtggattt ctttatcggt
      601 gaattcttaa aaatgggcga tattactcaa ggcgtgagct ttgaatttgt cggtatggtt
      661 acagggccgt taattgcggt atggctgatt gctattgctg tgttggctat ggggatcaaa
      721 aaagggattt ctgctgcctc aaacctgcta atgccgcagt tgatcgtgat gttcttagtg
      781 ttggtcggct attcattaac cctaccgggt gctgcgaaag gcttagatgc gttatttacc
      841 ccaaactggg aaaaattggc tgatccaagt gtgtggattg ccgcttacgg tcaaatcttc
      901 ttctcactct ctatctgctt cgggattatg attacctatg cttcatacct gaaaaaagaa
      961 accgatttaa ccggttcagg cttagtggtg ggttttgcga acagtggttt tgaattatta
     1021 gcgggtattg gcgtatttgc ggcactcggc tttatggcaa cggctgcagg gcaagaagtg
     1081 aacgaggtag caaaaggcgg tatcggcttg gcattcttcg ctttcccaac cattattaac
     1141 caagcaccaa tgggggcatt agttggcgtg ctgttctttg gttcactcac ctttgcggca
     1201 ttaacctcat ttatctcggt aattgaagtg attatttcag cggtgcaaga caaactcaat
     1261 tcaggtcgtg tgaaagccac gctactagtc ggcattccga tgatggtggt atctgtcttg
     1321 ctattcggta caaccaccgg tttaccaatg cttgatgtga tggataaatt cgtaaaccaa
     1381 ttcggtattg ttgcggtggc gtttgtttcc ttgattgcga ttgtcaccag cgagaaatta
     1441 agcacccttg aaaaacacct gaacgaagtt tcttcattca aagtcggctt tatctggcgt
     1501 ttatgtatcg ttattacctc cggcgtactg gcatttatgt tattcagcga aatcgccaaa
     1561 gtgtatcgtg aaggttatga aggctaccca agctggtttg taaacacctt cggctgggga
     1621 atggcaatcg gcttagtggt ggtggcttat ttgctatcaa aaatggcgtg gaaaaatgaa
     1681 ccaaaaggag atgagtaatg agttcagcag caattattac catggtggtg gcaatgacga
     1741 ttatctgggg cggtttactt tttgcgatta aacgtctgcc gaaagaagaa caataatttg
     1801 caaatttttg tataaaaaag cccgcttgtt agcgggcttt tggctatacg gtaatcccaa
     1861 atttctcttc cacaaaggct tggaaatcgg tgcaaccgcc aacgtggtta ttatccacaa
     1921 aaatttgtgg tacagttgca accggcttgc caacacgagg ctctaggtct tgttttgaaa
     1981 tattttcagc gatcatatca ataaatttaa aatcaaaatc gcttaattct tgtttcattt
     2041 tctcggctaa tgtttttgca cgcacgcagt atgggcaaga taaacgtcca taaatttcaa
     2101 caaacatata atgttccttc tataaagtaa aagtgagcga attgtccgtt aaacggctct
     2161 gtgtaagtgt aacaattttt tcaacctttt aggataaatt tcttcaccat cacggcaagc
     2221 agttaatgtg atatactggg gcaaatttac aagtagagaa acagagatga cccaattaat
     2281 gacgtttgag gagcttgatt taagccctca attacttaaa gccttagcac acaaaggcta
     2341 taaccgccca accgctattc aacaagccac gattccggct gcgttagaag gacgtgatct
     2401 gctcggttca gcccctaccg gtaccggcaa aaccgccgct tttttactgc cggcaattca
     2461 gcatttatta gattatcctc gccgcaaacc aggggctcct cgcattttaa ttcttacccc
     2521 aacccgagag ctggcaatgc aggtggcgga gcaagcccaa gcactggcta aatttactca
     2581 gttgagcatt gcgactatta ccggtggggt ggcgtatcaa aatcacggtg aagtctttaa
     2641 taaaaatcaa gatattgtgg ttgctacccc gggtcgttta atgcaataca ttaaagagga
     2701 aaattttgac tgccgagcgg tggaagtgct gatttttgat gaagcagata gaatgttgca
     2761 aatgggcttc gggcaagatg cggagaaaat tgcggcagaa acccgttggc gtaagcatac
     2821 ttggttgttt tcagcgaccc tcgagggcga gttattagag gattttgccg agcgattatt
     2881 aaatgatccg attaaagtcg atgccgaacc aagtcgccgt gagcgtaaaa aaattcagca
     2941 atggtattac cacgccgata atgtagagca taaaaccaaa ttattagctc gtattatcag
     3001 cgaattttcg gtggaaagag cgattgtgtt tgtccgccgc cgagaagatg tgcgagaact
     3061 cagcgaaacc ttgcgtaagc ggggaattcg ttctacttat ctcgaaggtg aaatggctca
     3121 gacccaacgt aaccaagcta ttaaccgttt aaaagaaggc gtagtgaatg tgttagtggc
     3181 aaccgatgtg gcggctcgag gcattgatat agacgatatt gatttcgtgc tgaactacga
     3241 tttaccttat agtgcggata cttacttgca ccgtattggg cgtaccgctc gtgctggcaa
     3301 aaaaggttcg gcaatttcat tggtggaagc tcacgattat aaattactcg gcaaaattaa
     3361 acgttacacc gaagaagtac taaaacctcg cattatcgag ggcttagagc ctcgcaccaa
     3421 agccccgaaa gatggcgagc taaatgtgac cagcaagaaa gaaaaagcac gcattaagaa
     3481 gaaaaaagag gcgaaaaaag aagcggcaaa accaaaagta aaagtccgcc acaaagatac
     3541 caaaaatatc ggtaaacggc gtaaaccaag tagtgaatcg gctgcggtta aacaggcata
     3601 atgatacaag cggttatttt ctgaagattt tttgcaaaaa tttcatcaaa aataaccgct
     3661 tgtttatatc taccttactg ccatttggaa cagaatcact tctgcttggc agctgaaagt
     3721 gatccacatt ttcatcagaa acccttctgc aatttgtgta atttcgcttt gaatttcaca
     3781 aggttcggtt tcgaccgatt ttgctttttg tgcgaaaaat tcgaccgctt gttgagccag
     3841 ttcttcgctc tcatacaccg cttcaaaggc aatttggcgg tctgcattat ccaacataga
     3901 atcgcctttt agccggcaca tattatgtgc ctcagccgct ttttgttgca tcgttttgct
     3961 catattacac cttatccgta atttgtttta accaagtgta ggattgatgc tgtttttgct
     4021 catcgaaaat gtagtttacc gccataattt catcaatttc cactcgtgct tttagggctt
     4081 taagttgttg ttcaacgctt tgcgggctac caattaagga gcattcggtc atttggcgga
     4141 caacggtttt ctcttgtcgg tatagagcat tttgctcaaa ggcaaccggc ccgaaatgcg
     4201 gcacaatttc tgctttcaca aaattttccc acactttgtc gtcagattcg agcggtggtt
     4261 gcagattttg ttgggcattg gtcactacat ttaagaaaaa ctgggtttgg gtggtcgcca
     4321 gttgtgttgc ttcttcatcg gtttctgcca caatggcatt cacacctaaa atcacataag
     4381 gtttatctaa cacatcagag ggcttaaaat gtgagcggta aatgcggatt gcatcgtcca
     4441 taaagcgagg ggcaaagtgt gaagcgaagg cgtaaggcaa gcctcgttca gccgctaaat
     4501 aggcactttc agggcttgag cctagaatat aaaacggcac atttagccct tgtgccggat
     4561 aggcggaaac agggtttgta ccgtcaaagt agccttttaa ttcgctgatt tcatcgggga
     4621 aaggcaaact ctgggcgtaa ggcagaggcg gttcgcatat cggtgccggg ggctcgccca
     4681 agccccagtt ctactcggtt tgggtagagt gtttcaagcg tgccgtattg ttctgccaca
     4741 atataagggc tgtggttggg taacatcacg ccaccggagc cgactcttaa tttttcggtg
     4801 tgggagaggg cgtgttgaat caataattgc gtggcggaac tggcaagatt tttcatatta
     4861 tggtgttcgg caatccaata gcgttcaatg ccgatatttt ctgcgtgttt tgctaatttc
     4921 accatagaat cgaccgcttg tttggcagtt tgcccttcac gaatcggcac aaggttgaga
     4981 atggataagt ttgtcatttt gttctccgag tttgcatata aaaaaggctg aatagcttta
     5041 ctattcagca cttgtaaaac catctcctta atatggggac gattatttct tactcaagaa
     5101 gaccacagcg tcaatatagc gttggataaa atcattggtc gagcttgaat cggaaaatcc
     5161 ttctagatta agctggtatt gaccattcac gaaaaacgcc ggtacaccac gaatttggaa
     5221 gtcttcagct aattgaactt gtttattgac taaaccgttt actgcaaagc tgttgatacc
     5281 gctgtcaaac tgttctgcac taatgctgtt agcgatgaag accgcacgaa tatcgtccat
     5341 tgatttaaac gcatcttttt gagcggcttc aaacaaggcg gtttttactt tatcttccgc
     5401 tcctaatgcc attgccaatg cccaagcacg ggttaagttt tcagattggc gacctaagaa
     5461 attgacgtga tattgcacta atttgctgtc tgcaggcaat ttcgctttga tttggcttgg
     5521 aattttgtaa tttagctcaa aatcgtaaca gtgggggcag tagaatgaga aaaattccac
     5581 cacttctttt tgagccgatg gggctttacg cacctcaata tactctttgc cggcagttgg
     5641 atctgctgca actgcggttg cgttaaatgc aaaaagggca gaaagagcaa ccagtacggt
     5701 ttttaatgca aattttttca taatgattcc ttcaaaaata tgataagtga gaaataagac
     5761 aaacttaatc gtttaaaatt ccacgagctc ggagcaatgc ggttttaaaa tcatcttcat
     5821 aatctttttt aatgcccgga atcggctcaa atttatcggc gtttcgcatt ttgagctgat
     5881 aaatcaaaac ctcgtcacgc agggcatgaa aattatccgg ctcgcttact tccaccgaaa
     5941 gctgttgcag aatatcaatt aagtgaagat cggggttttt ccgccaatgt tcgccaagca
     6001 gttccaatag ctcttctaaa cgtttgcatt tcatagtatg cctcttaaaa aacttgttga
     6061 atagtaagcg gaataatata cttttagccc tcatttggca aaaaaattca ggtttggaga
     6121 gggaaatgga agagaaaatt tcagcggtaa ttttagccgg aggtttggct cggcgaatga
     6181 atggagcgga aaaagggttg caactgttag atcaaaaacc gttgatttcg cacctgttag
     6241 cccgactttc gccacaaatc gaggatattt ggctcaatat caatcgttcg gtggaacaat
     6301 atcaacaact ttaccctgaa ttaccttatt accaagacag cttgcccgat tttcaaggcc
     6361 cactaagtgg aatgctttcc gggttcgagc agattgagag cgactattta ctgtttgtgc
     6421 cttgtgacac gccgtttatt ccaacccaac tattgcacaa gttacacacg gcattacgca
     6481 ttaatcaggc tcaaattgcc tatgctcacg atggcgaccg cccacaccca acggttgcct
     6541 tggtgcatcg ttcggtggcg gaaaatttaa gagcgtattt agcctccggc gaacgccgat
     6601 tattgtggtt ttttcaaacc caaaaaagtg tggcagtgga tttctcggca tataaagccg
     6661 catttcgcaa ttttaattgt ttagaagatt taacggcttt caatccatat cccatcaaaa
     6721 ccctggcgat tactggctat agcggcacag gcaaaaccac cttattggaa aaattaatcc
     6781 caaaattgac cgcttgcggt attcgggttg gcttgattaa gcattcccac cacaatgttg
     6841 atattgataa aaaaggaaaa gacagctatc gcttgcgaga agcaggagca aacccgacta
     6901 tggtcgtgtg tgatgaacgt tgggcattaa tgacggaaac taagcaggca gcagaaacta
     6961 aacaagcggt cgaattttca caattaattg caaaatttga cccagagacg gtagatttag
     7021 ttttagtgga aggctttaag cacgaaacaa tgccgaaaat tcaactacac cgtaaagaaa
     7081 ttgaaaaacc gttgccggcg ttagaccagt ggacaatcgc gaccgcaacc gattatccgc
     7141 ttgagcggga aaatcggttg gatattaatg atgttgaaca gcttgctgat tttattcggc
     7201 agtggctaga ggcaagcaaa gagccggcta attaattgac taaaaagccc ctaaagtaag
     7261 cgattgctta gtttaggggc taatagcaat taccagctat atcccagttt gaagccggca
     7321 aaatgttgtt tgccgatttc gttacctttc aatagaccaa ggtgagagct taattgccat
     7381 tgaccttttg taacacccac gcccacttca gttttcagat aacgtccgaa acgttggcta
     7441 aaggtattgt cattgactgc cacactcaag cgtttgctgc tggcatcgac ataatgtgcg
     7501 gcaaggctag gctcaacttt ccagctggag aggtcaaagg ccttgctgac tttcacacct
     7561 gcttggtagg ccataaagtg tgctttcggg ctgcggactt ctacttcacc cagtttgtaa
     7621 ttttgtgcgc tgaagcgata gtaacgtgta ccaacagcag gctgaacttg tacgccggct
     7681 aagtcgaagt tatgtccaag atttgcccca atcgccataa tatgacggtt aaagcggttc
     7741 tccccgaaca ggtcgatacg gtttttcgct ttaccgtagc taccgtctag gctgacgaaa
     7801 gtgccgtttt ccgaatgcca tttaccatat aggcttgcca tcagtaagtt ggatttaccg
     7861 ttcactccct catcaaattc cgcattagcg tggttgcggg atagcatcac accggcattg
     7921 ataccgtttg caagcggtaa ttctatgcct aactgagtga ggttggtttg ttgctcatac
     7981 gggcgatata aatccgattg gtgttcggtt ttttgatgtt cgatactgct ccaaacgtgg
     8041 aatttatcgt gttttgcgaa cagttggcga tctaagtctt gcccgatttg caacgcagaa
     8101 tttgcctgtg ccgatagctc ggaaagagcg gtattggcat attggcttac ccagtcgctt
     8161 tgttgaatat tactactgtc agtgtctgcg tgtgcttgta gatgctcaca atttgcacca
     8221 ttctcttggc aaagttttag tgcctcagcg tttaaaattg cttcactatt tccagcagtt
     8281 atacgtaagg tagctaattt tgcttctact tgttttgcaa tattttgtgc agtttcaacc
     8341 gcttgttcta cgttagcaat ttgcgcagcg gatgctttag cattgttttc tgctgtagtg
     8401 agtgcagcac tcgcttgtgt gactttctgt tttgctaccg taatttgccc ttggatttgg
     8461 cggtaatacg ttggatagta acggcggtag taatcagcat attgttgcaa acgggttaat
     8521 tcgctatttg cttggctaag ctgagattgt gcgtttgcag cggcttgttt agcatttttc
     8581 tctgcttggc gctctttctc ggcttctgcg ttcaaacggc tgatttcctg tttttgttta
     8641 tccgcttctg caatagcatg gtctaattcc gctttcgccg ttgcaatcga ctgattccga
     8701 tctttagcat cttttaacgg attgtataag cgatagccgt tgcttcgatt tgccagcaca
     8761 taacgatatg cacctaaatc aacataaccg ttttccagtg aaactttagc tttatcagcc
     8821 tgagcctgat gggttaaggt taataaactt aacgggctaa ccacttcagc ttctttgcca
     8881 ctgttgcgta atgcaagagt gaaagtcccc tcggcgagac catttacttg taggtgatcg
     8941 cctttgccag cagcaatatc ggttaagtag ttgattttgg cattaccttg gaaattaccg
     9001 tcaataatca acgtattaaa acgagtcggt atctcgcctg ttacaaattt ttcattcagg
     9061 ttgatggttg aaccgttttc tgccactaaa ttgccagtac ggctatcccc agtgtttgtc
     9121 cagcttgctt ggttagctaa acgaatgctc gttgttgctg ctgcttgaat ggtgccaatc
     9181 aggttcgctt tgccgatgtg taattggctc tgttctgcca agctgacatc accattaatt
     9241 tgtgttgtgg gcagtgttgc aaaaatttca ttagaaacga ccgcttgtgt atcacagctt
     9301 gttttaccag tgtactcaga atagtagcaa cttggagttt gctcgttgat aaagcctaaa
     9361 tgtagctgag catggtcatt cgctgacaag tttgcttgca ggtttgagac attacgacca
     9421 acataaagct gagcgtattg gtttagctgc atattatcag ccttaaattg acgattttgc
     9481 caatcatcgg cataaactac atcttgctta ttgatcgcat cataagcgtg aggggtcggt
     9541 ctgccggaaa gtagcacttt acctcctttc acagttagat cgccgtttaa gttcaaacca
     9601 ccagagaagg tataaatact attgagcttt tcttgctcta ttggtgcgtt tttacgggct
     9661 aatactttgt tgatcgcttg attgcgatct gcagctaaaa attcccaatg ctcgctgctt
     9721 aagccaccgc gaggaacagg ttgttttgga tcacctttta atgcaaagta ttcccgaatt
     9781 ttcgttttcg gattggtgaa cagataaata tctgcaccag ctactagccc cttaccccaa
     9841 gttacttcag aggcagttgc cggtggtgta gaggcttttg gatcatacac cacattcaaa
     9901 cgaccgttat gctgggtgtt atctgtttcg ccaataaaac cattaaagct attgtagcga
     9961 tgctccagac ctttttgcgc gagtacttgt tttactgctg catccttgtt agaagacagg
    10021 aaggtccaat gggcattact tgcgccattc gttggcatat attggtttgg attgcctttg
    10081 agcgtaaagt aatcggtacg gttattagca tgtgggttga tatattcata aatatcagca
    10141 ctgttttctt tccacgttcc ccattttaag tccgttgctt cagggggctt cgggccatta
    10201 atggtgagtg ttgcagcttt ttgtaggtga ttattaacaa tacgcgcacc atcatcacta
    10261 ttttgaatac gattaaacgc aatgttgttg ccgttgagat ctaaacgacc accacggaag
    10321 ccaaagtaaa tcttatttgg atcgacttga tcagcactgt ttaatacaac ggttgaacgg
    10381 ccgctcacaa taccaacttg gttaaatgca gattttttgc cattttcacc cgcttgttgg
    10441 gtcaaaatga cagtgccttg tccaacacta atgtcaccaa ggttttcgcc tttaccgtta
    10501 atatgtagtg tcccttcgcc caatttagat aaacggtcac cttgtgggtt tttgacttgc
    10561 caattaactt gtttaccttt tgccactaca attccagctc ctacccagct ttgatcattt
    10621 tcagggcgaa ctgttgcatt agcattgaaa tttaatgcgc cagcaccttg gtcaatatcg
    10681 gtttttaata cgatagtgct atcttgtcct tcaatgttta gggtttttcc ggtgttatct
    10741 tgcggaagtt gtagctgagg tgtgccacca tagcctaatg gagaagcgct attgtcttta
    10801 attgaacgat cagcaagcga aaccgttaag tttttccctt tattaccaat gctactgtct
    10861 ttaccctgtg cagaccattc aaaaacggca ttttgttgcg cattggttaa tgtgccggca
    10921 atatcttcgg caaattgttt atcgtgataa tcctgacgga taataccggc acggttgtag
    10981 tagttgtttg caggcgtacc aaaggtgtaa gtagccagta gtagccagcg tttcgttttc
    11041 tgatcgtagc catacacacc tgaaccgcta tcaccacgag taccaaatgc agttaatgct
    11101 ccatatgggc tattgtttaa tgcatttcct ctaaaatcta gccagtaatc ggaattatgt
    11161 accgcgattg gtaaatgaac tcccccggtt aaatattggt aagatgttga aatttggttg
    11221 tctttatgct cggtattttt gactatttga cgaccagagc ctaagcgagc aaagtgagta
    11281 aaacgactac tatcttggta ggctgaagca ttattaccca gatcagtcac tgttgcaggt
    11341 gcgacctcag tgactaattt attgagacgt ggcacttgat aatcttgatg ttgcccacct
    11401 tcaacaattc catagtcgtt acgatcaacg actttgtaag tgtagtggtg tgcatcggca
    11461 ttagtgcctg gggcgccaaa ttccacagta ttgtattgtg tattatgggc aacgctgact
    11521 aaatattgcg gtgcaactag agttgctatc cctaagttac ggtttgcagc actaaaatct
    11581 ggcatcggta catttgggaa gagtgcatct aatgtattgc cacttttatc tttaatttca
    11641 atattgcttg cacctacggt gaatttaccc ttattctccg caaaatcacg gtagtattga
    11701 agatcgatat catctctgaa aacagaggct gttgcagata aactaacagc aaatgcc
//
LOCUS       DANLUQ010000070        11755 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.070, whole genome shotgun sequence.
ACCESSION   DANLUQ010000070 DANLUQ010000000
VERSION     DANLUQ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11755
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.070"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            66..323
                     /locus_tag="PXB27_001453"
     CDS             66..323
                     /locus_tag="PXB27_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL5346232.1"
                     /translation="MKNLRLSIHSSEHIWLRELFLKRRLELGLTQRTLGEKMGVLYSF
                     IGKVETGDRRLDIFEFIAYCKGLDLDPLSVLQEIEQQFPHK"
     gene            complement(370..990)
                     /locus_tag="PXB27_001454"
     CDS             complement(370..990)
                     /locus_tag="PXB27_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HDL5346233.1"
                     /translation="MKLWYSTTSPFARKALAVIKHHQLDDKVEMLRVTKAMDPNSPHN
                     QDNPLGRIPALQRNCGRWLFGSLLISEYLDQKGNNTPLLPKEGKPRWAVLALHNLADG
                     IMENTMPTLLQERMARPENEWWVSRHEQLMERNIRSFRQLEEALQEFGTELNLGTLTA
                     VALIDWWAFRLDKIGYDLAKNYPNLTAWAEEMNNKYAILAETKPSM"
     gene            complement(992..1645)
                     /locus_tag="PXB27_001455"
     CDS             complement(992..1645)
                     /locus_tag="PXB27_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
                     /protein_id="HDL5346234.1"
                     /translation="MTPTLPICYQHRDFIIINKPEGLSVHKDEAEIGLTELVARQLNV
                     KQVWLVHRLDKITSGLLILALNKEAAARFYHLFEQHKIYKTYWALSDKKPKKKQGRIV
                     GDMQKSRNGAWKLCHSKENPAVTQFISHSVEDSLRHFILQPKTGKTHQLRVAMKSLGS
                     PILGDKLYSGSQADRVYLHAYQLDFEYENEKISVQALPTSGHFWQKICKSNHIKHGE"
     gene            1694..2353
                     /locus_tag="PXB27_001456"
     CDS             1694..2353
                     /locus_tag="PXB27_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL5346235.1"
                     /translation="MAVTIYLVRHGRTVWNLEGRLQGSGDSALVEEGIIGAKKTGIAL
                     KHIPFTAAYSSMQKRAQDTANYILAENERSNIPHFHHKGLNEFDFGSWEGMKSVDLQE
                     NDEYWVMKRTPAEYLAKANGGERFEQLYQRVTQAFNQIAELHQNDGKVLIVSHGMTLT
                     LLTAVLKGIAWQDFRNEEKHSFVFNTAITKVEVENGKVTVLEFNNTDHLDNVPTSQYK
                     R"
     gene            complement(2390..3436)
                     /locus_tag="PXB27_001457"
     CDS             complement(2390..3436)
                     /locus_tag="PXB27_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HDL5346236.1"
                     /translation="MSKQIFNEEELKTTEHFEPKQEFDLENAIIEEEEKAVEAELIIE
                     ESLKPSRFWIRVLLATLALFGVAVIAQSIQWLIDTFQQREWIYFAFSIVFFIVSLLGV
                     GAIISEWRKLVYLRNHQKNQQMSNQLLLEQLPSNDGEQAVKFCENIANQLNHLPSIAQ
                     AEQLWKSELNEAYNAKEVLYLFSENVLKPIDKQVKQMISRNAAENAVIVAVSPLAIVD
                     VLLMAARNIALVNKITKAYGMELGYISRLKLFKMVLKNMVFAGATEIATDVGMDFFSQ
                     NLTAKLSLRAAQGIGVGLLTARLGIKAMEFCRPVAFQANEKPRISAIRQELLTSVKNT
                     VFAKTETKEKVFSD"
     gene            complement(3436..4389)
                     /gene="dusC"
                     /locus_tag="PXB27_001458"
     CDS             complement(3436..4389)
                     /gene="dusC"
                     /locus_tag="PXB27_001458"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HDL5346237.1"
                     /translation="MNPIPNVILAPMQGVLDPFVRNLLTSVNDYDLCISEFVRVVDQR
                     LPKKTFYRLAPELYQGGLTDSGTPVRVQLLGQHPQWLAENAALAIELGSHGVDLNCGC
                     PSKTVNGSNGGASLLKDPELIYRATKAMREAVPSDKIVSVKVRLGWDSVSQCFEIADA
                     VEQGGANEITVHGRTKVDGYRAERINWQAIGEIQQRLSIPVIANGEIWDFESAKNCQK
                     ITACKALMMGRGALNTPNLSKVVKYNVPKMAWNEVLQLLFKYVQMENQFDTGFYHVAR
                     IKQWLRYLDKEYPQAVELFDILKTEHGYDGLKAHIEKAVEE"
     gene            complement(4467..5093)
                     /locus_tag="PXB27_001459"
     CDS             complement(4467..5093)
                     /locus_tag="PXB27_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HDL5346238.1"
                     /translation="MDSRSHIIPLFLAIVITVIAVWSGLNPTDRAVWYAEVIPIFIVF
                     ALLIITYPKFQFSGLAYFLMSLWLIMHLIGAKYTFANVPFEWANQFLTPILGEDRNHF
                     DRVAHYIIGFYSFPMAEWLLRKRKCGMGVAFFFSLFFIMSVAAAYEIIEWQYAVIEGG
                     NAGVEFLGSQGDIWDAQKDMLADTLGALTALMIYLIARPDLRIHDRHF"
     gene            complement(5093..5950)
                     /gene="djlA"
                     /locus_tag="PXB27_001460"
     CDS             complement(5093..5950)
                     /gene="djlA"
                     /locus_tag="PXB27_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HDL5346239.1"
                     /translation="MQFIGKIVGFFLGYQLFHGLFGGILGAFIGHLADKKLYELGSVR
                     SSLFGKNLTRQSLFTQTTFAVLGHIAKAKGRVTEADIELARALMARLKLDSAAQQLAQ
                     NAFTLGKEPDFPLRQVIQEFREACGARTDLLRFFVEVQMQAALQDGQLDVNEQQILFT
                     IAETMGMSRFQFEQMIAMIMAAQQFRNGGYYQQNGSYHQQSQGGYQGSYQQPAQPSIE
                     AAYTVLGVTAQDDQNTVKRAYRKLMNEHHPDKLAAKGLPDEMMELAKEKAQQIQAAYD
                     LICKVKEWK"
     gene            complement(5998..6708)
                     /locus_tag="PXB27_001461"
     CDS             complement(5998..6708)
                     /locus_tag="PXB27_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL5346240.1"
                     /translation="MWKNRQLFFGYAENRLLLLSRSITGLLGILCGVYIIDHLVLSDV
                     DMIGKLTSFILIILSAIFLKERASLLQWILCLIAFIGALFIIKPAVDIRFMPYLVGVI
                     GSVFAAIAYLCLRLLAKTKKVESPNTIVFFFSAFSTLILLPFVAIDYVVLTNLQTIYL
                     VLAGVTSAVGQFCVTTAYKYAAAKDISIYSYASVLFSAILGLLFFEQYPDMLSWLGYA
                     IIFVSSWLMFVLTKKQSK"
     gene            complement(6723..6875)
                     /locus_tag="PXB27_001462"
     CDS             complement(6723..6875)
                     /locus_tag="PXB27_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346241.1"
                     /translation="MNFWNSLSPLFKAIFCVLLSALSFAIMAAFLRLSGDVPIAQKAI
                     FRNAQH"
     gene            complement(6944..7429)
                     /locus_tag="PXB27_001463"
     CDS             complement(6944..7429)
                     /locus_tag="PXB27_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDL5346242.1"
                     /translation="MKKFAKVLMVAVPAFALAACSSSSDDAAANAAANAAANAAANAA
                     GEFGGLTAEQLKAQYNTVYFGFDSYAVEGEYRQLLDAHAQLLAKTQGNVTIAGHADER
                     GTPEYNIALGQRRADAVKGYLAAKGANNTSTVSYGEEKPAVLGHSEADYAKNRRAVLE
                     Y"
     gene            complement(7456..8742)
                     /gene="tolB"
                     /locus_tag="PXB27_001464"
     CDS             complement(7456..8742)
                     /gene="tolB"
                     /locus_tag="PXB27_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HDL5346243.1"
                     /translation="MKLFSRISGVIGIFSALLTTQVVADSDVRISIDEGVSMAQPIAV
                     VPFKASGVPADVAQIISDDLRNSGKFTPLERSQMPAQPGSAAEVVADQWSAIGIDNIV
                     VGQVSSTGGGYNVAYQLIDTLGTSGTAGGVIAQGSFIVPAAQIRQGAHTVSDEVFEKL
                     TQIRGAFRTKIAYVVQRGVSSYELRVSDYDGFNAFTITTSKEPLMSPEWSPDGSRLAY
                     VTFENKKAQVVVHDIRSGARRVVAALKGHNGAPSFSPDGSRIAFASNQDGELNIYVVG
                     SGGGTPSKLTANAGNNTEPSWSPDGSTIYFTSDRAGSPQVYRMSSSGGGASPMGGSGS
                     YNAKVSSDGKNLIMIAGDRVVKRDLASGGTEVLSSTFLDESPSISPNGIMVIYSSTKG
                     TSKVLQLVSADGRFKANLPGAGGHYKFPAWSPYLTK"
     gene            complement(8758..9981)
                     /gene="tolA"
                     /locus_tag="PXB27_001465"
     CDS             complement(8758..9981)
                     /gene="tolA"
                     /locus_tag="PXB27_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HDL5346244.1"
                     /translation="MKSQQDRLELAVIVSILLHILLIGLLLLGSLFTKTELAAAGGSG
                     GDSDSFEAVMVDTGQVAAEYGQLLAEKKGSKASKPKVEPKEEAVEEDVEDKPTPEEIQ
                     KQKQAELAAIQEKQRQQEIERQQKLQEQQKQEQAKQEELKKQQAEEATRKKAAEAAKL
                     KADAEAKRLEAAAKQAEEEKKAKEALEKKLEQQKKAEAEKKLKEQKELKEQQAKEAKE
                     AKEKAEKEAKAKAEKEAKEKAEKEAKAKAEKEAKEKAEKEAKAKAAAEAKAAQAKNNK
                     ALDDFLNSGDIGGGSSKGGNKNSAGSQGSGGTSGLGQGTNVDGNAYGQRIKKLIQSRY
                     RVDPSFAGKQCDVKIFLSRDGTITNYQVISGDKAVCDAAVSAIVATRKVPPAPSDAVY
                     NMFKSPTLDFSLKVK"
     gene            complement(9995..10432)
                     /gene="tolR"
                     /locus_tag="PXB27_001466"
     CDS             complement(9995..10432)
                     /gene="tolR"
                     /locus_tag="PXB27_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HDL5346245.1"
                     /translation="MSRRLKRNDIKSEINIVPFLDVLLVLLLIFMATAPIISQSVEVN
                     LPEDTHSQSVSNEDKTPVILEVAGVGEYKLKIDGNYVQSNGQENINESEVVAYAGEQF
                     QKDNNTLFLVAGDKVVPYEEIMKGITLLKDAGVKSVGLMTEGK"
     gene            complement(10574..11254)
                     /gene="tolQ"
                     /locus_tag="PXB27_001467"
     CDS             complement(10574..11254)
                     /gene="tolQ"
                     /locus_tag="PXB27_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HDL5346246.1"
                     /translation="MQTEFSLLSLFLEASIVVKIVMLILIVFSILSWAVIIQRSKILT
                     KAKKDSLAFEDRFWSGEDLNRLHQGLENRRDALNGSEQIFYVGFKEFTRLQQANPDAP
                     ESIIQGSTRAMNLALNREMESLENYIPFLGTVGSISPYIGLFGTVWGIMHSFMGLSAV
                     KQATLQSVAPGIAEALIATAIGLFAAIPAVMAYNRLNLRLGKVEQGYVNFIDEFTTLL
                     HRQAFSKR"
     gene            complement(11276..11677)
                     /gene="ybgC"
                     /locus_tag="PXB27_001468"
     CDS             complement(11276..11677)
                     /gene="ybgC"
                     /locus_tag="PXB27_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HDL5346247.1"
                     /translation="MDFPIRVYYEDTDAGGVVYHSNYLNFFERARTEFLRQQHFSQQE
                     LFSQSLAFVVKRIEIDYKLPARLDDLLNVETTLSELKKATLIFKQRLWKDGDCLSEAN
                     VTVACVDLIKMKPVAIPDDIRQALQKSYFSI"
BASE COUNT         3403 a         2642 c         2145 g         3565 t
ORIGIN      
        1 ctacaaatta attatataat gaacctaaaa aaggttcaac attatttgta cctaatatag
       61 gtgcaatgaa aaatcttaga ttatcaatac attccagcga gcatatttgg ttaagagaac
      121 tattcttaaa acgccgtttg gaactagggc taacacaacg cacattagga gaaaaaatgg
      181 gcgttcttta ctcatttatc ggaaaagtag aaacaggaga tcgcagatta gatatttttg
      241 aatttatcgc ttattgcaaa gggttagatt tagatccttt atctgtattg caagaaattg
      301 aacaacaatt tcctcataaa taaaggcatt gagttataac tcaatgcctt tattttctat
      361 gtcaaatctc tacatgctcg gcttagtctc tgccagaatg gcgtatttat tattcatttc
      421 ctctgcccaa gcggttaaat ttgggtaatt ttttgcaaga tcgtagccta tcttatctaa
      481 gcggaatgcc caccagtcaa ttaatgctac tgcggttaat gtgcctaaat tcagctctgt
      541 gccaaactct tgcaatgcct cttctaattg tctgaacgaa cggatattgc gttccattaa
      601 ttgctcgtgg cggctgaccc accattcatt ctcggggcga gccatacgtt cttgtaataa
      661 agttggcatc gtattttcca taatgccgtc cgccaaatta tgtaatgcca acactgccca
      721 gcgaggttta ccctctttag gtaacagtgg agtgttattg cctttttggt caagatattc
      781 gctaattaac aaactaccga aaagccaacg tccgcagttg cgttgtaggg caggaatacg
      841 tcctaacggg ttatcttgat tatgtggcga atttggatcc attgcttttg tcacccgcaa
      901 catttcgact ttatcgtcta attggtggtg tttaatcacc gcaagggctt tgcgggcgaa
      961 agggctggtg gttgagtacc aaagtttcat actattctcc gtgtttgatg tgattggatt
     1021 tgcaaatttt ttgccaaaaa tgaccgcttg tcggcaaggc ttgcactgag attttctcat
     1081 tttcgtattc aaaatcaagc tgataagcgt gcaaataaac acggtcggct tggctacctg
     1141 aatagagttt atcacctaaa atcgggctgc ccaaactttt cattgccact cttaattggt
     1201 gagttttgcc tgttttgggt tgcaaaataa aatgcctgag cgagtcttca acagaatgag
     1261 aaataaattg cgttactgcc gggttttcct tactgtggca taatttccac gctccatttc
     1321 tgcttttttg catatcgcca acaattctac cctgcttttt cttcggcttt ttatcgctta
     1381 atgcccaata ggttttgtag attttatgct gttcaaataa atggtaaaac cttgctgccg
     1441 cctctttatt caacgctaaa atcagtaaac ctgaggtgat tttatccaga cggtgaacca
     1501 gccaaacctg ctttacattc agttggcgag ccactaattc cgttaagccg atttcagcct
     1561 catctttatg cacactcaag ccttccggct tattgataat aataaaatca cgatgttgat
     1621 aacaaatagg caatgttggg gtcattttgc tataatcctt ccaatttttg actattttag
     1681 caaggaaatt acaatggctg ttaccattta tttagtacga cacggcagaa ccgtgtggaa
     1741 tttggaaggc agattacaag gctcgggcga ttccgcttta gtagaagaag ggattatcgg
     1801 agcgaagaaa accggcattg cgttaaaaca tattccgttt accgccgcct attccagtat
     1861 gcagaaacgg gctcaagata ccgcaaacta cattttagca gaaaatgaac gcagtaatat
     1921 tccgcatttt catcataaag gcttgaatga atttgatttt ggcagctggg aaggaatgaa
     1981 atctgtcgat ttgcaagaaa atgatgaata ttgggtgatg aaacgtacac cggcagaata
     2041 tctggcaaaa gcaaacggtg gcgagcgatt tgagcaactt tatcaacggg tgactcaggc
     2101 gttcaatcaa atcgccgagc tacaccaaaa tgacggcaaa gtgctgattg tttcacatgg
     2161 tatgacctta accttactca cggctgtgtt aaaaggcatt gcgtggcaag atttccgcaa
     2221 tgaagaaaaa catagctttg tgttcaatac tgccattacg aaagtagaag tagagaatgg
     2281 caaagtaacg gtgctggaat ttaataatac cgaccatttg gataatgtgc caacaagcca
     2341 atacaaacga taatattaag ggcggaacat tgtccgccca tagcattttc taatcagaga
     2401 acactttttc tttcgtttct gttttagcaa ataccgtatt tttgaccgaa gtaagcaact
     2461 cctgacgaat agctgaaatt cttggcttct cattcgcttg aaaagcaaca ggacgacaaa
     2521 attccattgc tttaatgcca aggcgagcag tgagtaaacc cacaccaata ccttgtgcgg
     2581 cacggagcga taatttcgcc gtaaggtttt gcgagaagaa atccattcca acatcagtag
     2641 cgatttcggt tgcaccggcg aacaccatat ttttcagcac cattttgaac agttttaagc
     2701 ggctgatata acccaattcc attccatagg ctttggtaat tttattcact aaagcgatat
     2761 ttcttgccgc cattaacaac acatcaacta ttgccagcgg gctaaccgcc acaatcaccg
     2821 cattttctgc cgcattgcgg gaaatcatct gcttaacctg tttatctatc ggctttagca
     2881 cattttcgct aaacagatat agtacctctt tggcattata agcctcgttc agttcgcttt
     2941 tccaaagttg ctcggcttga gcaatgctcg gcaagtgatt aagctgattt gcaatatttt
     3001 cgcaaaattt gaccgcttgt tcgccatcat tggaaggcag ctgttccagc aataattggt
     3061 tactcatttg ttgatttttc tgatgatttc gcaaatacac caactttcgc cattcggaaa
     3121 taatcgcccc aacgcccaac aagctgacaa taaaaaagac tatcgagaac gcaaaataga
     3181 tccactctct ttgctggaac gtatcaatca accactgaat actttgagca attaccgcca
     3241 cgccaaacaa ggcaagtgtg gcaagtagta ctcgaatcca aaagcgggaa ggcttaaggc
     3301 tttcttcaat aatcagctcc gcctcaactg ctttttcctc ttcttcaata atcgcatttt
     3361 cgagatcaaa ctcttgttta ggttcaaaat gctcggttgt ttttagctct tcttcattaa
     3421 aaatttgctt actcattatt cctccactgc cttttcaata tgtgctttta gaccatcata
     3481 gccgtgttcg gttttcaaga tatcaaataa ttctaccgct tgaggatatt ctttatccaa
     3541 ataacgtagc cattgtttga tgcgagcaac gtggtaaaaa ccggtatcaa actgattttc
     3601 catctgaacg tacttaaaca atagctgtag cacttcattc cacgccattt tcggaacatt
     3661 atatttcacc actttgctta aattcggtgt gtttaacgcc ccacgcccca tcattaatgc
     3721 cttgcaagcg gttatttttt ggcaattttt cgccgattca aaatcccaaa tttcgccgtt
     3781 ggcgataaca ggaatagaca gccgttgttg aatttcgcca atcgcctgcc aattaattct
     3841 ttctgctcga taaccatcaa ctttggttcg cccgtgaacg gtaatttcat tcgctccgcc
     3901 ctgttctacc gcatcggcaa tttcaaaaca ttggctaacg gaatcccaac ctaatcgcac
     3961 ttttacggaa acgattttat cgcttggcac agcttcacgc atcgctttgg ttgcacgata
     4021 aatcagttcc ggatctttta ataacgaagc cccaccattg ctgccattta ccgttttaga
     4081 ggggcagcca cagttgagat ctaccccgtg tgaaccaagc tcaatggcaa gagctgcatt
     4141 ttctgccagc cactgtgggt gttgccctaa aagctgtacc cgcactggtg tgccggaatc
     4201 ggttaatccg ccttgatata gctcaggggc taggcgataa aaggttttct tcggtaaacg
     4261 ttgatcgacc actcgcacaa attcggaaat acagagatcg tagtcattca ctgaagttaa
     4321 aaggttgcgg acaaaagggt ctaaaacccc ttgcatcggg gcaagaataa cgttaggaat
     4381 tggattcatt ggtaaaataa aaaatacaag cggttatttt ttctcaacat tttgcaaaaa
     4441 attggcaaga aacaaccgct tgaaggttaa aaatgtctat cgtgaatgcg caaatcgggg
     4501 cgagcgatca ggtaaatcat cagtgcggtt aatgccccaa gagtatccgc aagcatatct
     4561 ttttgagcgt cccaaatatc cccttgcgag cctagaaatt ccacgccggc attgccacct
     4621 tcaatcacgg catattgcca ttcaataatt tcgtaagctg ccgccaccga cataatgaaa
     4681 aacagcgaga agaaaaatgc tacgcccatt ccgcacttgc gtttacgcag taaccattct
     4741 gccatcggga agctgtaaaa accgataatg taatgtgcta ctcgatcaaa atggttacga
     4801 tcttcaccta aaatcggggt tagaaattga tttgcccact caaacggcac attggcaaag
     4861 gtatattttg ccccgatcaa gtgcataatc agccaaagcg acatcaaaaa ataagctaac
     4921 ccactaaact gaaatttagg gtaagtgata attaaaaggg caaaaacgat aaaaatcggt
     4981 atcacttccg cataccaaac tgcgcggtct gtcggattta accctgacca aaccgcaatc
     5041 acggtaataa caatcgctaa aaatagaggg atgatatgcg agcgtgaatc cattatttcc
     5101 actctttcac tttacaaatt aaatcgtacg ccgcttgaat ttgttgagct ttctcttttg
     5161 ccaactccat catctcatca ggcaaacctt tagcggctaa tttatccggg tgatgttcgt
     5221 tcattaactt acgataagcc cgtttcactg tattttgatc gtcttgtgcg gttacgccta
     5281 atacggtata ggctgcctca atgcttggtt gtgcaggctg ctgataactg ccctgatagc
     5341 caccttgaga ttgctgatga taactcccat tttgctgata atagccacca ttgcggaatt
     5401 gttgagctgc cataatcatt gcgatcatct gctcaaattg gaagcgagac attcccattg
     5461 tttcggcaat cgtaaacaga atttgttgct cgttcacatc aagttgccca tcttgcaagg
     5521 ctgcctgcat ttgcacttcg acaaagaaac gtaataagtc agttcttgcc ccacaggctt
     5581 ctctgaactc ttgaatgact tggcgtaacg ggaagtcagg ctctttacct aaggtaaagg
     5641 cattttgcgc taattgctgg gctgcactat ctaattttaa gcgagccatt aacgctcggg
     5701 ctaattcaat atccgcttca gtaacacgcc cttttgcttt cgcaatatgc cccaacaccg
     5761 caaaagtggt ttgggtgaaa agcgattggc gagttaaatt tttaccgaaa agacttgaac
     5821 gaactgaacc taattcatag agttttttat cggctaaatg cccgataaaa gcgccaagta
     5881 tcccaccaaa taaaccgtga aaaagctgat agcccaagaa aaaaccgact atttttccga
     5941 taaattgcat ttttttatcc ttttatagaa atgaataaac gttattctaa caaaatttta
     6001 ctttgattgc tttttagtta atacaaacat aagccagctt gagacaaaaa taattgcata
     6061 acctagccaa ctcaacatat ccgggtattg ttcaaagaac aacaacccta aaattgcact
     6121 aaatagcaca gaggcataac tataaattga gatgtctttc gccgccgcat atttataggc
     6181 ggtagttaca caaaactgcc cgaccgctga agttacccct gctaacacta agtagattgt
     6241 ctgtaaattg gttagcacta cataatcaat ggcgacaaaa ggcaataata tcaaggtcga
     6301 aaaggcagaa aaaaagaaaa caatggtatt aggtgattct acttttttgg tttttgccaa
     6361 taaccttaag cataaatagg caatggctgc aaacacagag ccaatcacgc caaccagata
     6421 aggcataaag cgaatatcaa ctgccggttt gataataaaa agtgccccga taaaggcaat
     6481 aaggcataaa atccattgca ggagagatgc tctctctttt aaaaaaatcg cagaaagaat
     6541 aattaaaatg aacgacgtga gtttgccaat catatcgaca tcacttagca caaggtgatc
     6601 gatgatatat acaccacata aaatccccaa taacccggtg atggaacggc ttaataataa
     6661 caaacgattt tctgcataac caaagaataa ttggcgattt ttccacacaa tataaccgct
     6721 tatcaatgct gtgcgttgcg aaaaatcgct ttttgtgcaa tcggtacatc ccccgaaaga
     6781 cgaagaaaag cagccataat cgcaaagctt agtgccgaca aaagcacaca aaaaatggct
     6841 ttaaataacg gcgagagcga attccaaaaa ttcatagcat aaaggctttt aataaaaata
     6901 aagccctgat tattcagggc ttcaatttag aatcaactaa gaattagtat tctaatactg
     6961 cacgacggtt tttcgcgtag tcagcttctg agtgacctaa tactgctggt ttctcttcac
     7021 cgtaagaaac agttgaagtg ttgttagcac ctttagctgc taagtaacct ttaactgcat
     7081 ctgcgcgacg ttgacctaat gcgatgttgt actctggagt accacgctca tcagcgtgac
     7141 cagcaatagt tacgttacct tgagtttttg ctaataattg agcgtgagcg tctaataatt
     7201 gacggtattc accttcaact gcatagctgt caaaaccgaa gtatacagtg ttgtattgtg
     7261 cttttaattg ttcagctgtt aaaccaccga attcgcctgc tgcatttgct gctgcatttg
     7321 ctgctgcgtt tgctgctgcg tttgctgctg catcatctga tgagctgcta catgctgcta
     7381 atgcgaaagc tggtactgct accattaata ctttagcaaa ttttttcatt tgttgctcct
     7441 aatagagaaa tattattatt tagttaagta tggtgaccat gcagggaatt tataatgtcc
     7501 acctgcgcca ggcaaattag ctttaaaacg cccatctgca gacaccaatt gtagcacttt
     7561 actcgtacct ttggtagagc tataaataac cataatgcca ttcggtgaaa tacttggact
     7621 ttcgtctaag aaagttgaac tcagtacttc tgtgccacct gaagcgagat cgcgtttcac
     7681 aactctatca ccggcaatca tgattaaatt cttaccatca gatgatactt tcgcattata
     7741 gctaccgcta ccgcccattg ggctagcacc accacctgat gaactcattc tataaacttg
     7801 aggagaacct gcacggtcag acgtaaagta gattgtgcta ccatctggag accagcttgg
     7861 ttcagtatta tttccagcat tagcggtcaa tttacttggt gtaccaccgc cactacctac
     7921 aacataaata tttaactcac catcttgatt tgaggcaaac gcaatgcgag aaccgtctgg
     7981 agagaatgat ggcgcaccat tatgtccttt caatgctgcc actacacggc gagcacctga
     8041 acgaatatcg tgaacaacaa cctgcgcttt tttattttca aatgtcacat aagctaatct
     8101 gctaccatct ggtgaccact ctggcgacat taaaggctct ttactggttg taatagtaaa
     8161 ggcattaaaa ccatcataat cagatactcg taattcatac gatgatacgc cacgttgaac
     8221 cacatacgca attttagttc taaatgcacc gcgaatttgg gttaattttt caaatacctc
     8281 atcacttact gtatgcgcac cttgacgaat ttgggctgcc ggaacaataa atgaaccttg
     8341 ggcaatgaca ccacccgctg tacccgatgt accaagagta tcaattaatt gataagcgac
     8401 attataaccg ccacctgtgc tactcacttg gccaactaca atattatcaa taccgatagc
     8461 actccattga tctgccacca cttccgctgc tgatcccggt tgagcaggca tttgagagcg
     8521 ttcaagcggt gtaaatttac cactatttcg caaatcatca gaaataattt gtgcaacatc
     8581 tgccggcaca ccgctggctt taaatggcac aaccgcaatc ggctgagcca tacttacacc
     8641 ttcatcaata gaaattcgca catcagaatc agcaaccacc tgagttgtca ataatgctga
     8701 aaagatgcct attacacccg atatacgaga gaataatttc atttgtagtc ctctaaatta
     8761 tttaactttt aaactaaaat caagtgttgg tgatttaaac atattatata ccgcatctga
     8821 tggtgcaggt ggtacttttc tagttgccac tattgcactt accgctgcat cacatactgc
     8881 tttatcgcca ctgataactt gatagttagt aattgtgcca tctcgactta agaatatctt
     8941 aacatcacat tgttttccgg caaaacttgg atcaacccga tatcgagatt gaattaattt
     9001 cttaattcgt tgaccatatg cattaccatc tacattagta ccttgcccta agccacttgt
     9061 accaccagaa ccttgtgatc ctgctgagtt tttgttacca cctttacttg aaccaccacc
     9121 gatgtctcca ctattgagga aatcatcaag tgctttattg tttttcgctt gggcggcttt
     9181 tgcctcagca gctgccttcg ctttagcttc tttctcggct ttctctttgg cttctttttc
     9241 tgcttttgct ttggcttctt tctcagcctt ttcttttgct tctttttctg ccttggcttt
     9301 agcttctttt tctgcttttt ccttcgcttc tttagcttct tttgcctgct gttccttcag
     9361 ttctttttgc tcttttaact ttttctcagc ttccgctttc ttctgctgtt caagcttctt
     9421 ttcaagtgct tctttagctt ttttctcttc ttcagcttgt ttagcagcag cctctaaacg
     9481 cttagcttct gcatctgctt tcagttttgc tgcttcagct gcttttttac gagttgcttc
     9541 ttctgcttgc tgctttttaa gctcttcttg cttagcttgt tcttgctttt gctgttcttg
     9601 tagtttttgc tgacgctcaa tttcttgctg acgctgtttc tcttgaatcg ccgctaactc
     9661 agcttgtttc tgtttttgga tctcttccgg agtcggttta tcttccacat cttcttcaac
     9721 agcttcctct ttaggctcta cctttggctt cgatgctttt gagccttttt tctctgccaa
     9781 taactgccca tattcagcag ccacttgccc agtatctacc atcacagctt caaagctatc
     9841 gctatcgcca ccactgccac cagccgcagc gagttccgtc ttagtgaaaa gagacccgag
     9901 caataacaac ccgattaaca atatatgcaa taagatcgaa acaataaccg ctagctctag
     9961 tctatcttgt tgagatttca cacgttatcc ttggctattt accttctgtc attaaaccta
    10021 ccgatttcac tccggcgtct ttaagtagtg taataccttt cataatttct tcataaggta
    10081 ctactttatc accggcaact aaaaataagg tattgttatc tttttgaaat tgttcccctg
    10141 cataggcaac aacttctgat tcattaatat tctcttgccc attactttgc acatagttac
    10201 cgtcaatttt caatttatat tcaccaacac ctgccacctc caaaatcact ggagttttat
    10261 cttcattaga aactgattgg ctatgcgtat cttccggtaa attaacttct acactttgac
    10321 tgataattgg ggccgttgcc ataaaaatca gcaataatac caacaataca tcaagaaatg
    10381 gaacaatgtt gatttctgat ttaatgtcat tacgttttaa acgacgagac atatattcct
    10441 ctttacaggt gcttagcacc ttaattgatc tgaaataacc ggcacagtgt cccggtctcg
    10501 aataatgcgg ttacaagcgg tcttttttac gaaaaacttt acaaattttc attaaaaatc
    10561 aaccgcttgt agattatctt tttgaaaaag cctgacggtg taataaggtc gtgaactcat
    10621 caatgaaatt cacataccct tgctcaactt tacctaaacg taaattcaaa cggttatatg
    10681 ccattactgc aggaatcgcc gcaaataaac cgatagccgt tgcaattaat gcttctgcaa
    10741 tacccggtgc gactgattgt aaagttgctt gcttaactgc acttaatccc ataaaggagt
    10801 gcataattcc ccaaaccgtt ccgaataaac caatatatgg gctgattgaa cccactgtgc
    10861 ctaaaaacgg aatgtagttt tctaaacttt ccatttcacg gtttaatgct aagttcatcg
    10921 cacgggtaga accttgaata atcgattctg gcgcatctgg gttcgcttgt tgtaaacggg
    10981 taaattcttt aaacccaaca taaaagattt gctctgaacc atttaatgca tcacgacgat
    11041 tctctaaacc ttggtgtaaa cggtttagat cttcccctga ccagaatcga tcttcaaaag
    11101 caagggaatc ttttttggct ttagttaaaa ttttgctgcg ctgaataatc accgcccaag
    11161 acaaaattga gaatacaata aggatcagca tcacgatctt taccacaata cttgcttcta
    11221 aaaataacga aagaagacta aattcagttt gcattaataa aactccgaat agtttttaaa
    11281 tagaaaaata acttttttgt aatgcttgac gaatatcatc aggaattgca accggcttca
    11341 ttttgattaa atcaacacaa gcgaccgtaa catttgcctc actcagacaa tcgccatctt
    11401 tccacaatcg ttgtttaaag ataagggttg ctttcttcaa ttctgagagt gttgtttcca
    11461 catttaataa atcatctaaa cgagcaggga gcttataatc tatttcaatc ctcttaacaa
    11521 caaaagcaag ggattggcta aaaagttcct gctgtgagaa atgctgctgt ctcaaaaatt
    11581 ccgttcttgc ccgctcaaaa aaattcagat agttggaatg ataaactact ccgccggcat
    11641 cagtatcttc ataataaaca cgaatcggga aatccataag cattatttct ctcattcaaa
    11701 atgaaaaaac gcccaaaatt taggcgcaaa ttgtgagata ttttaggatc agaaa
//
LOCUS       DANLUQ010000071        11749 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.071, whole genome shotgun sequence.
ACCESSION   DANLUQ010000071 DANLUQ010000000
VERSION     DANLUQ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11749
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.071"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            81..578
                     /gene="purE"
                     /locus_tag="PXB27_001469"
     CDS             81..578
                     /gene="purE"
                     /locus_tag="PXB27_001469"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDL5346248.1"
                     /translation="MPNKAEIAIVMGSKSDWATMSEATQILDQFGLSYHVEVVSAHRT
                     PDKLFSFAETAETNGYKVIIAGAGGAAHLPGMIAAKTIVPVLGVPVKSSMLSGVDSLY
                     SIVQMPKGIPVGTLAIGPAGAANAGLLAAQILAAFNPDIAEKLRIFRQTQTQMVLDNP
                     DPRVE"
     gene            593..1558
                     /locus_tag="PXB27_001470"
     CDS             593..1558
                     /locus_tag="PXB27_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005607504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDL5346249.1"
                     /translation="MSKKLLILHTGGTISMKEGEDGKVSPSNENPLLAALERLNHPAK
                     LAQESLFNIPSPHINIEHWQQLKMRIEKAVNEENVDGVVITHGTDTLEETSYFLDLAL
                     SVNIPVVITGAMRSSNELGADGLINLQSAILVALNEESRDKGVLVVMNDEIHNAKFVT
                     KTHTTNVATFQTPTFGPCGLVTKESVIFFQKLTTYERYPIQQVTKANVQLLKAYAGMD
                     SFLLEQLAKSKCDGVVIEALGAGNLPPSCLAGIEALVNANIPIVIVSRAFNGITQDVY
                     DYVGGGKQLKQKGIIFTKGLSGQKARIKLMVLLSQTLDKPLSDYF"
     gene            1577..2665
                     /gene="purK"
                     /locus_tag="PXB27_001471"
     CDS             1577..2665
                     /gene="purK"
                     /locus_tag="PXB27_001471"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDL5346250.1"
                     /translation="MQKSAIYPPVYVLGNGQLGRMLRYAGAPLDIEVKPLAFDAPVFD
                     IEPNSIITAEIERWADTPLTQLLGNHPNFVNLNVFGKLADRFTQKSLLDELHLATSKW
                     QLLENAEHWQDIFQHIGEKVVVKRRTGGYDGRGQWIVTQDSIHQITPELFGEVIAEKF
                     IPFEGEISIVGARFRDGSTRFYPISHNLQQNGILRYSVSDVNLPNQALYQQQAEQMLA
                     TVMQKLEYVGVMAMECFVVGDKLLINELAPRVHNSGHWTQLGCAISQFELHLRALLDL
                     PTPALDQIAPSVMVNLIGIEHNKQWLSLPFSQLHWYGKEVRAGRKVGHINLTHTDKNG
                     LIELLEQLRPSLTEDFHSGLDWAINKLK"
     gene            complement(2886..3296)
                     /locus_tag="PXB27_001472"
     CDS             complement(2886..3296)
                     /locus_tag="PXB27_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346251.1"
                     /translation="MISRSLFSKLILFAKIVIFSQLVFMVVIWIAHHIMREYSNPFER
                     FGTLFNNVFLTFIIPMMVSSAVIVYKGYTQKNEDKVFWIYMGFFLIWILGFIYFEPAV
                     YDKSLKISGAGFGAMLSIIICSKLLPKETNKEQL"
     gene            complement(3299..5317)
                     /gene="uvrB"
                     /locus_tag="PXB27_001473"
     CDS             complement(3299..5317)
                     /gene="uvrB"
                     /locus_tag="PXB27_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HDL5346252.1"
                     /translation="MSKQGKPFILHSPFKPSGDQPSAIAKLTEGLNDGLAHQTLLGVT
                     GSGKTFTIANVIAKLNRPAMVLAPNKTLAAQLYAEMKAFFPENAVEYFVSYYDYYQPE
                     AYVPASDTFIEKDASINEQIEQMRLSATKSFLERRDTIVVASVSAIYGLGDVDAYMQM
                     MLHLQVGAMIGQREILSRLAELQYTRNDQAFQRSTFRVRGDVIDIYPAESDEVALRVE
                     LFDDEIENLSLFDPLTGHSLGKVPRYTIYPKTHYVTPRERILEAIEQIKVELADRRTY
                     FIKENKLLEEQRIAQRTQFDIEMMNELGYCSGIENYSRYLSGRKEGEAPPTLFDYMPA
                     DGILVIDESHVTVPQIGGMYRGDRARKETLVQYGFRLPSALDNRPLRFEEFERLSPQT
                     IYVSATPGAYELEKNPDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIHKRVAVDER
                     VLVTTLTKKMAEDLTDYLDEHGVRVRYLHSDIDTVERVEIIHDLRMGMFDVLVGINLL
                     REGLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNLNGKAILYGDRITNSMQKA
                     ITETERRREKQMKYNEEHGITPQALNKKVGELLDIGQSDKPKRGKQAVKKAENSATDY
                     KPKSRKELEKELKTLEQQMRDFAKDLEFEKAAAVRDKLQHLKAYLLEV"
     gene            complement(5556..5792)
                     /locus_tag="PXB27_001474"
     CDS             complement(5556..5792)
                     /locus_tag="PXB27_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HDL5346253.1"
                     /translation="MSRTTSVTLAEPLSQFVNQMVESGRYGSTNEVISAALKILEAQE
                     QQLIQLRALIDEGLESGVSEETVQSIISKAKRNV"
     gene            5955..6800
                     /locus_tag="PXB27_001475"
     CDS             5955..6800
                     /locus_tag="PXB27_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005823485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EfeM/EfeO family lipoprotein"
                     /protein_id="HDL5346254.1"
                     /translation="MFLLKGNFYAFNQTCFCTFALSGFFVATQAAALDLTKETEAYKQ
                     FVVEQIDQLVTDTEKFVGYLNKGDVQKAKKIYPLARMYFERSEPIAESFGDLDPRIDA
                     RLADLAEEGKTEKDWSGFHKIEKVLWQKNTTKGTKATAEQLLKDVKELRAKIPTAEVT
                     PEPMITGAVDLLNEVSTTKVTGEEEIFSKTDLYDFKANIEGAEKIYEIFKTQLEQIDA
                     KLSTEIGSRFAAVNALLAKHNKSKTGYDYVPYNKLSKADIKALAEAVNQLGEPLAQLG
                     VLLNK"
     gene            6811..8007
                     /gene="efeB"
                     /locus_tag="PXB27_001476"
     CDS             6811..8007
                     /gene="efeB"
                     /locus_tag="PXB27_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HDL5346255.1"
                     /translation="MMAETTSRRDFLKHTALVGAGLLSAPAFALESRQISEKSQNQYD
                     FYGKHQAGIVSPAQKHIYFLVLDLDTTDIAKVKTLFQNWTEYSRNLTLGKNVKPYSAN
                     AYVPPADTGEADSLSAQNLTLTFGVSANFFTKLGIEKHKPKELNDLPHFPRDQLQAEY
                     TGGDICIQACADDPQVAFHAVRNLVRVARGEIKMKWSQMGFNSFANQDTPRNLFGFKD
                     GTANAESLKDQENTVWVQQEGWLKGGSYLAVRRVKMFLETWDRTHLQSQEETFGRHRD
                     SGAGIGHQHEFDKLDLAKKDEKGNAVIPEVSHAHLANKTGLKILRRSFSYSSGIDDKG
                     QFDSGLLFVSFQQSPEQFIKIQNALGNVDKMNEYITHIGSGLFACFAGVKDENDYLGK
                     ALFDLI"
     gene            8018..8637
                     /locus_tag="PXB27_001477"
                     /pseudo
     CDS             8018..8637
                     /locus_tag="PXB27_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596976.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+/Pb2+ permease"
     gene            complement(8673..9491)
                     /locus_tag="PXB27_001478"
     CDS             complement(8673..9491)
                     /locus_tag="PXB27_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDL5346256.1"
                     /translation="MKYDLHAHSTASDGVLSPTELVQRAVEQGVTMLALTDHDTISGI
                     QEAKNFAQNQPLELISGVEISILWQEKSIHLAALNIDENNEELVKFLDNQAQLREERA
                     VEIGEKLAKVGIANAYEGAKALATGEVTRAHYGRFLYEQGYVRNIEHAFKRYLGMGKP
                     AYVKPRWSSLEDAVNVTHTSGGVISIAHPLRYKMTARWIRRLIADFKQAGGDGIEVSG
                     CGQTPDQRQLLARWAKEFDLYGSVGSDFHYPTGWIELGKSLELPQECKPIWTLF"
     gene            complement(9503..10102)
                     /locus_tag="PXB27_001479"
     CDS             complement(9503..10102)
                     /locus_tag="PXB27_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HDL5346257.1"
                     /translation="MTMDNLPLEQHPFDPILPEQATVVMVGTFPPTSEKRCMEFHYPN
                     FQNDMWRIMGNIFYADPNYFRVGEEKRFDPIRIEAFLREKGVALCSSAKTAIRLKGNA
                     SDKDLKIVDPIDMDELLLKLPKLKWLFTTGGLATDTLLGLLPEKHKAPKTNEWVVYPY
                     SATRELYLYRLPSTSRAYPLSLANKTEAYRQFFRTAGLI"
     gene            complement(10099..11646)
                     /gene="sbcB"
                     /locus_tag="PXB27_001480"
     CDS             complement(10099..11646)
                     /gene="sbcB"
                     /locus_tag="PXB27_001480"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HDL5346258.1"
                     /translation="MSDKFSFFFYDYESFGVSPALDRPAQFAGIRTDADFNIIGEPVM
                     FFCKQTPDYLPSPEAVMVTGITPQQCNAEGVSEPEFAARIHAEFSRANTCTIGYNNIR
                     YDDEMTRYTFFRNFYDPYEYSWKNGNSRWDLLDLVRACYALRPDGIEWPTDETGMPSF
                     KLENLTKANGIAHENAHDAMSDVYATIAMAKLIKQKQPKLFDFFFNLRSKKEVEKLID
                     TGEMTPLVHVSGMLGNYRGNTAWVVPLAWHSTNQNAVIVCDLSGDMNGLLTENAEVLR
                     QRLYTKRDELAENELPVPLKLVHINKCPILAPAKTLLPENAERLGIDRALCLENLKTL
                     KAQKSLIREKVIEIFNEERTFEPSTNVETTLYDGFFSPADKNNMAILRTLPPEDLANH
                     GLKFEDPRVEALLFHYRARHYPQTLSRAEQIKWQKHCNQQIEAKAAQFAQSIDDLFQQ
                     HHDNPEKVKLLENLTAYAEQISQQQAVIYRQNVAKDEKLLSELNRVAEQPLDKTEKLK
                     MLKELIK"
BASE COUNT         3416 a         2575 c         2451 g         3307 t
ORIGIN      
        1 cacttcttaa atagctacgc aaacgtttgc tttgtatgct atgattacac tgttttacat
       61 tagtatcata aggatttaaa atgccaaata aagctgaaat tgcgatcgta atgggatcta
      121 agagtgattg ggctacaatg tctgaagcaa cccaaatatt agaccagttc ggtttgagct
      181 atcacgttga agtcgtctca gcacaccgca caccggacaa actcttttct tttgctgaaa
      241 ctgcagagac taacggctat aaagtgatta ttgccggagc cggcggtgca gctcacttgc
      301 ccggaatgat cgctgctaaa accattgtgc cggtgttagg tgtgccggta aaaagctcaa
      361 tgttaagtgg tgtagatagc ctctattcga ttgtgcaaat gccgaaaggt attccggtcg
      421 gtacgctcgc aatcgggccg gcaggtgcag caaatgcagg cttattagcc gctcaaattt
      481 tagcagcctt taaccctgat attgcagaga aactgcgtat tttccgtcaa acccaaactc
      541 agatggtatt agataatcct gatcctcgtg ttgaataact attgtataaa agatgtctaa
      601 aaaattatta atcctccaca ctggtggtac tatttcaatg aaagaagggg aagacggtaa
      661 ggtctcccct tccaatgaaa atcctctctt ggctgcctta gaacgtttaa accacccagc
      721 aaaattggcg caagaatcgt tatttaatat tccctctccg catatcaata tcgagcattg
      781 gcaacagtta aaaatgcgga ttgaaaaggc agtgaatgag gagaatgtcg atggcgtagt
      841 aatcacccac ggcacagata ccttggaaga aacctcctat tttctggatt tagccctcag
      901 cgtaaatata ccggtagtaa ttaccggagc aatgcgttcc agtaacgaac tgggtgctga
      961 tggcttaatc aatctacaaa gtgcgatttt agtggcgtta aatgaagaaa gccgagataa
     1021 aggcgtgtta gtggtgatga atgatgaaat ccacaatgct aaatttgtga ccaaaaccca
     1081 caccactaat gtggcgactt tccaaacccc aacctttggg ccttgtggct tagtaacgaa
     1141 agaatctgtc atttttttcc aaaaattgac cacttacgaa cgttatccaa ttcaacaagt
     1201 aactaaagct aacgtgcaac tactgaaagc atacgccgga atggatagtt ttctgctgga
     1261 acaattagcg aaaagcaaat gcgatggtgt tgtgattgag gcattgggtg caggtaattt
     1321 accccctagt tgtctggctg gtattgaggc tttagtgaat gccaatattc caattgtaat
     1381 tgtttctcgg gcattcaatg gtattaccca agatgtctat gattatgtag gcggtggaaa
     1441 acagttaaag caaaaaggca ttatttttac caaaggctta agtgggcaaa aagctcgcat
     1501 taaacttatg gtcttactca gccaaacctt agacaaacca ctatcagact atttctaacc
     1561 cattgagaat accacgatgc aaaaaagtgc aatctaccca cctgtgtatg tattaggcaa
     1621 cggccagctc gggagaatgc tacgctacgc cggagcacca cttgatattg aagtcaaacc
     1681 gctggctttc gatgcccctg tgtttgatat tgagccaaac agcattatta ccgcagaaat
     1741 cgaacgttgg gcagatacgc ctttaacgca attactcggt aaccacccga attttgtgaa
     1801 cctgaatgtt tttgggaagt tagccgatcg ttttacgcaa aaatcactac ttgatgaact
     1861 ccatcttgcg acttcaaaat ggcaactgct agaaaatgcc gaacattggc aagatatttt
     1921 tcagcatatt ggcgaaaaag tagtggttaa acgccgaacc ggaggatatg acggacgtgg
     1981 gcaatggatt gtaacgcaag actcgattca tcaaattaca cctgaattat ttggcgaagt
     2041 gattgccgaa aaatttatcc cgtttgaggg ggaaatttcc attgttgggg cgagattccg
     2101 tgacggctca acccgtttct accctatttc tcacaattta caacaaaacg gcattctacg
     2161 ttactccgtc tccgatgtga atttaccgaa ccaagccctc taccagcagc aagccgaaca
     2221 gatgctcgct acggtaatgc aaaaattaga gtatgtcggc gtaatggcaa tggaatgctt
     2281 tgttgtgggc gacaagctac tcatcaacga gcttgcccct cgagtgcata acagcggaca
     2341 ctggacacaa ctcggctgtg ccattagcca atttgagtta catttacggg cattattaga
     2401 tctacctacg ccagcactcg accaaattgc cccaagcgtg atggttaatt taataggcat
     2461 tgaacataac aagcagtggc taagcctccc atttagccaa ctacattggt atggcaaaga
     2521 agtccgagct ggtagaaaag ttgggcatat caacctcact cacaccgata aaaacggctt
     2581 aattgaacta ctggaacaac tcagaccgag cctaacggaa gatttccatt cgggattaga
     2641 ttgggcaatc aataagctca agtaagcggt cggattttgt aacttttttg caaatctgtt
     2701 tacccgatgg tgcgagcttc ctcgctcgta ccaaacaagc ggtcgactta gtgctaaaat
     2761 ttgcaaaata attcaacata atggctactt ccggtcatca tcttgatctg aaaacccctc
     2821 agggtggata tggctatcaa aaagcatttt cttcctccga gaaaacagat ttcttcacaa
     2881 gaatcttaga gctgctcttt atttgtttcc ttaggcaata acttcgagca aatgataata
     2941 gagagcatag ccccaaatcc tgcacctgaa atcttcagtg atttatcata aactgccggc
     3001 tcaaaataaa taaatcccaa aatccaaatt aaaaagaacc ccatataaat ccaaaacact
     3061 ttatcttcat ttttctgggt ataaccttta taaacaataa ccgcactact taccatcatc
     3121 ggaataataa aagtcaaaaa cacattattg aacagggtac caaatcgttc aaacggatta
     3181 ctgtactccc gcataatatg gtgggcaatc cagataacca ccataaaaac aagttgagag
     3241 aaaataacaa ttttggcaaa taagattaat tttgaaaata atgaacgaga aatcatattt
     3301 aaacctccaa caaatacgcc tttaaatgct gcaacttatc ccttaccgca gccgcttttt
     3361 caaattccag atctttcgca aaatcacgca tttgttgttc cagcgtttta agctcttttt
     3421 ccagttcttt acgggatttc ggcttgtagt cggttgcaga attttcggct tttttgaccg
     3481 cttgtttgcc ccgttttggc ttgtcggatt gtccgatgtc gagcaattca cccacttttt
     3541 tgttcaacgc ttgtggcgtg atgccgtgtt cttcgttgta tttcatctgc ttttcacggc
     3601 ggcgttcggt ctcagtaatt gctttttgca ttgagttggt aatgcggtcg ccgtataaaa
     3661 tcgcttttcc gttgaggttt cgggcggcac gtccgatggt ctgaattaac gagcgttcgg
     3721 aacgtaaaaa tccctcttta tccgcatcaa gaatggcaac cagcgacact tcgggcatat
     3781 ctaagccctc acgcaataag ttgatcccca ccagcacatc aaacattccc attcgtaaat
     3841 cgtggataat ttcgacccgt tccaccgtgt caatgtcgct atgtagataa cgaactcgta
     3901 cgccatgttc gtccaaataa tcggtgagat cttccgccat ttttttggtg agtgttgtca
     3961 ccagcacccg ttcgtccacc gccactcgtt tgtgaatttc agacagtaaa tcgtccacct
     4021 gcgtagcaac ggggcgaact tcgataatcg gatcgagcaa gccggttgga cgaactactt
     4081 gatccaccac atcgggattt ttctccaatt catacgcccc cggtgtggca gaaacgtaaa
     4141 tcgtctgtgg tgaaagacgt tcaaactctt caaatctcaa cgggcggtta tccagtgcag
     4201 acggcagacg gaaaccgtat tgcaccaatg tttcttttct cgctcggtcg cctcgataca
     4261 ttccgccaat ttgtggcaca gtaacgtggc tttcatcaat caccaaaata ccgtccgccg
     4321 gcatataatc aaacagggtc ggaggggctt cgccctcttt tctgcccgag agatagcgtg
     4381 agtagttttc aatgcccgaa cagtagccca gctcgttcat catctcaata tcaaactgcg
     4441 tacgttgggc gatccgctgc tcttccagca atttattctc tttgatgaaa taggtgcgac
     4501 ggtcggcaag ctcgacctta atttgctcga ttgcctccaa aatccgttcc cgaggggtca
     4561 catagtgagt tttcgggtaa atggtatagc gaggcacctt gcccaagcta tgccctgtaa
     4621 gcggatcaaa cagcgacaga ttttcgattt catcgtcaaa cagctctacc cttaacgcta
     4681 cttcatcgct ttcggcaggg taaatatcaa tcacatcgcc ccgcactcgg aaagtcgaac
     4741 gttggaacgc ctgatcgttg cgagtatatt gcaattcggc aaggcgagag agaatttcac
     4801 gctgcccaat catcgccccc acttgcaaat gcaacatcat ctgcatataa gcgtccacat
     4861 cacccaagcc gtaaatggca gaaacggacg ccactacaat cgtatctcgg cgttccaaaa
     4921 aggatttggt tgcggaaagt cgcatttgct cgatctgctc attgatagag gcatctttct
     4981 cgataaaggt atcgcttgcc ggcacatagg cttcgggctg gtagtaatca tagtaagaga
     5041 cgaaatactc caccgcattt tccggaaaga acgctttcat ctcggcataa agctgggcgg
     5101 caagggtttt attcggggca agcaccatcg ccgggcgatt gagcttggca atcacattag
     5161 caatggtaaa ggttttcccc gagccggtta cgccaagtaa ggtttgatgt gccaagccgt
     5221 catttaagcc ttcggtcaat ttggcaatcg cacttggttg gtcgccggag ggcttgaaag
     5281 gagaatggag gataaagggt ttgccttgct tgctcataga aaatcctgtc ttaaaaaatc
     5341 ggtttatttt agcacaagca agcggtcgtt tttatcggga tttttgcaaa tttattgtaa
     5401 ctgcttatat tgatccattc ttggatctaa caccctaaca ataaagatac cttttgggat
     5461 tggtttataa taaatagaat gtgagcggtg gtaatgacag cgtaacaaag ggcgaataaa
     5521 gtaacgctaa atctcgctct gccctaacag aaagtttata cattacgttt tgccttgctg
     5581 ataatagatt gaacggtttc ctcagaaaca ccgctttcta agccttcatc aattaaggct
     5641 cttagctgaa ttagttgctg ttcttgtgct tccaatattt ttaatgcggc actgatgacc
     5701 tcgttggtcg aaccataacg cccactttca accatttggt tcacaaattg gcttaatggc
     5761 tcagctaaag ttacgctggt tgttctactc attactattc ctatcttatt gattattgag
     5821 cagattgtaa tggctctaat ttagataagc aagcggtcta tttttagcaa taatttgcaa
     5881 aacaaaatgt gatttacatc acaaaatcag cattattttt tgcgaataat tcgcaataag
     5941 gatattatgt cgcaatattt ttattaaaag gaaatttcta tgcgtttaac caaacttgct
     6001 tttgcacttt tgcactttct ggcttttttg tggctactca agcggcagct ttagacttaa
     6061 ctaaggaaac cgaggcttat aaacaatttg tggtagaaca gattgaccaa ctggttaccg
     6121 atacggaaaa atttgtcggc tacttaaaca aaggcgatgt gcaaaaagcg aagaaaattt
     6181 acccgcttgc aagaatgtat tttgaacgtt ctgaaccgat tgcagaaagt tttggcgatt
     6241 tagaccctcg tattgatgcc cgtctggcag atttagccga agaaggtaaa accgagaaag
     6301 attggtcagg tttccataaa atcgagaaag tattgtggca gaaaaacacc actaaaggca
     6361 caaaagcaac ggcagaacaa ttattaaaag atgtaaaaga gctgcgtgcg aaaattccca
     6421 ctgcagaagt tacccctgag ccgatgatta ccggtgcggt agatttatta aacgaagttt
     6481 ctactactaa agtaaccggt gaagaagaaa ttttctctaa aaccgacctt tacgatttta
     6541 aagccaatat tgaaggtgca gaaaaaatct atgagatttt taaaacgcaa ttagagcaaa
     6601 ttgatgccaa actctcaaca gaaatcggct ctcgttttgc ggctgtcaat gcgctgttag
     6661 caaaacacaa taaatctaaa accggttatg attatgtgcc ttataacaaa ctgtcaaaag
     6721 cggatattaa agcgttagca gaggctgtaa accaattagg cgagccgtta gctcaacttg
     6781 gcgtactttt aaataaatag tggagcaaaa atgatggcag aaaccacttc tcgccgtgat
     6841 tttttaaaac ataccgcatt agtaggagca ggcttattgt ctgctcctgc tttcgctttg
     6901 gaatcacgac aaatatctga aaaatctcaa aatcaatacg acttttacgg caaacatcaa
     6961 gctggtattg ttagccctgc acaaaaacat atctactttt tagtgctgga tttagacacc
     7021 actgacattg ccaaagtaaa aacattgttt caaaactgga cggaatatag ccgtaacctc
     7081 acgcttggca aaaatgtgaa accttatagt gctaatgctt atgttccacc ggcggatacc
     7141 ggtgaggcag atagcctttc ggcacagaat ctcactctta cttttggcgt cagtgccaat
     7201 ttctttacaa agctcggcat tgaaaaacat aagccgaaag aactgaatga tttaccgcat
     7261 ttccctcgtg atcaactaca agcagaatac accggtggcg atatttgcat tcaagcctgt
     7321 gcagacgatc cgcaagtggc tttccacgct gtgcgtaatt tggtgcgtgt tgcccgtggt
     7381 gaaatcaaaa tgaaatggtc gcaaatgggg tttaactcct ttgccaatca agacacacct
     7441 cgtaacttat tcggttttaa agacggcact gccaacgcag aaagtttaaa agatcaggaa
     7501 aatacggtat gggtgcaaca agagggctgg ctcaaaggcg gttcttattt agccgttcgc
     7561 cgtgttaaaa tgttcttgga aacttgggat cgcacccatc tccaatccca agaagaaacc
     7621 ttcggtcgtc accgtgattc cggagccgga atcgggcatc agcacgaatt tgataaatta
     7681 gatttagcca aaaaagacga aaaaggcaat gcagtaattc cggaagtttc acacgctcat
     7741 cttgccaata aaacggggct gaaaatccta cgccgttcat tctcctattc aagcggtatt
     7801 gatgataaag ggcaatttga ttcagggttg ttgtttgtct ctttccaaca aagtccggag
     7861 caatttatca aaatccagaa cgctctcggc aatgtggata aaatgaatga atacatcact
     7921 catatcggca gtggtttatt tgcctgtttt gccggtgtga aagatgaaaa tgactacctt
     7981 ggcaaagcgt tgtttgattt aatttaggga aaaggaaatg aaattaaaag cattagtgct
     8041 aattccgtta ctcttttcga gtacaggatt gcttgccaaa gtgaatgtca gccccttatt
     8101 tgttcagctt tccgatgcaa tggcagagag taaaaaaggg gaatttgcaa aatccagcca
     8161 aaatttgacc gctttacagc acgattttga gcagattagc cagcacgatt ccctcgccgg
     8221 agttgaagta cagaccgctc tgaaaaaagc gattgaaaca cccaatgctg aaaacttaga
     8281 gagcctttct aaaaactcta tgcgtttgaa aaagaacaaa accctagcgg ttatgctgaa
     8341 aaacatcagg attttgtggc aaaaatgacg ccgctttatg cagaacttgc tcaaaccgtg
     8401 ccaactcaag aaatcgccaa aatcaaatgg gcggcttatc agtttggtaa gaattgggta
     8461 aaacaggaaa aagcagtacg ggaaatcagt cttccccatt atggcaaatt tgagcgtatt
     8521 cttggtttaa tgcgaattaa tctgaacgct gaaaagccgg atatggcaaa aatcagtgaa
     8581 ctggtttccg aactaggtgt ggtaatggca gactttaagc aagttaaagt gaaatagtaa
     8641 aaaatcgggg caaattattt gccccgataa cactaaaata acgtccaaat cggtttacat
     8701 tcttggggta attccaaact tttaccgagc tcaatccagc cagttgggta gtggaaatca
     8761 gaaccgaccg agccatataa atcaaactct tttgcccaac gggcaagtaa ttgccgttga
     8821 tcaggtgttt gtccgcagcc tgatacttca atgccatcgc ctccggcttg tttgaaatct
     8881 gcgattaaac ggcgaatcca acgagcagtc attttatagc gtagcgggtg agcaatcgaa
     8941 atcacaccgc ctgaggtgtg ggtaacattc accgcgtctt ctaaactact ccatctcggt
     9001 ttcacatagg caggtttccc cattcccaga taacgcttaa aggcgtgttc aatattgcgg
     9061 acatagcctt gttcatacaa aaaacgccca taatgtgccc gagttacttc ccctgttgcc
     9121 aacgcttttg ccccctcata ggcatttgca atcccgactt ttgctaattt ttcaccaatt
     9181 tcgaccgctc tctcttccct taattgggct tggttatcca aaaacttcac cagctcctcg
     9241 ttgttttcat caatattcaa cgccgcaaga tgaatacttt tttcttgcca taaaatcgaa
     9301 atttccaccc cagagataag ctcaagcggt tgattttggg caaaattttt cgcttcttga
     9361 atgcccgaaa tcgtatcgtg atcagttaaa gctagcattg ttaccccttg ctctaccgca
     9421 cgttgtacca attcagtggg agaaagtacg ccatcagagg cggtactgtg ggcgtgtaaa
     9481 tcatatttca ttttatttat ccttaaatta agccggcagt cctgaaaaat tggcgatagg
     9541 cttcggtttt gttggctaag ctgagtggat aagccctcga cgtcgaaggc aggcgatata
     9601 aataaagttc tcttgtggcg gaataaggat agaccaccca ttcgttggtt ttcggagctt
     9661 tgtgtttttc cggcaataaa ccgagcaacg tatcggttgc caaaccacct gtggtaaata
     9721 gccatttcag tttgggtaat ttgagcaaca gttcgtccat atcgatagga tcaacaattt
     9781 ttaaatcttt atctgaagcg tttcctttta aacgaatcgc tgtttttgcc gaagaacaga
     9841 gcgcaacgcc cttttctcgc aaaaacgcct caatccgaat cggatcaaag cgtttttctt
     9901 caccaactcg aaaatagtta gggtcagcat aaaaaatatt gcccataatt cgccacatat
     9961 cattttgaaa attcgggtaa tgaaattcca tacaacgctt ttccgatgta ggtggaaaag
    10021 tgccgaccat taccaccgtt gcttgttcag gtaaaatagg gtcaaacggg tgctgttcta
    10081 gcggtaaatt atccattgtc atttaatcaa ctcttttaac atcttgagct tttcggtttt
    10141 atctaggggc tgttccgcta ctctgttaag ctcgctcagc aatttttcat cttttgcaac
    10201 attttgacga taaatgaccg cttgttgttg cgaaatctgc tcggcgtaag cggtcaaatt
    10261 ttctaataat tttacctttt ccgggttgtc gtgatgttgt tggaacaagt cgtcaatcga
    10321 ttgggcgaac tgcgctgctt ttgcctcaat ttgctggttg caatgtttct gccatttgat
    10381 ctgctcggct cgactgagtg tttgtggata gtgccttgct cggtaatgga atagtaacgc
    10441 ctctactcga gggtcttcaa atttcaagcc gtggttggct aaatcttcag gcggtagcgt
    10501 gcgtaaaatc gccatattat ttttatccgc aggcgagaaa aagccgtcat acaaagtggt
    10561 ttctacattg gtcgaaggct caaacgtgcg ttcttcatta aaaatttcaa tcactttttc
    10621 ccgaatcagc gatttctgtg cttttaaagt tttcaaattt tccaaacata aagctctgtc
    10681 tatgcctaaa cgttctgcat tttcaggcag taaggttttt gccggtgcta aaatcgggca
    10741 tttattaata tgcactaatt ttaacggcac aggtaactca ttttcggcta attcatctcg
    10801 tttggtgtat aaacgctgac gtaaaacctc tgcattttca gtcaataagc cattcatatc
    10861 gcccgataaa tcacacacaa ttacggcatt ttgattagtt gagtgccaag ccagtggcac
    10921 aacccaagcg gtatttcctc gatagttgcc cagcataccg gaaacgtgca caagcggtgt
    10981 catttcacca gtgtcaatta acttctctac ttctttttta ctgcgtaaat taaagaaaaa
    11041 atcgaacaac tttggctgtt tttgcttaat taattttgcc attgcgatgg tggcatacac
    11101 atcagacatc gcatcgtgtg cattttcatg ggcaatgcca ttggctttgg tgaggttttc
    11161 caatttaaaa cttggcatac cggtttcatc tgttggccat tcaataccat caggtcttaa
    11221 cgcataacag gcacggacta aatctaacaa gtcccaacga gaatttccgt ttttccagct
    11281 atattcgtaa ggatcgtaaa aattgcggaa gaaggtataa cgggtcatct catcatcata
    11341 acgaatattg ttataaccga tagtacaagt attcgctcgg ctaaattcag cgtgaattcg
    11401 ggctgcaaat tccggctcag atacaccttc agcgttacat tgttgcggtg taatcccggt
    11461 aaccatcacc gcttcggggc tgggtaaata atccggcgtt tgcttacaaa aaaacattac
    11521 cggttcaccg ataatattaa aatccgcatc ggttcgaata ccggcaaatt gtgccggacg
    11581 atctaacgcc ggactcacgc cgaaactttc ataatcgtaa aagaaaaaag agaatttatc
    11641 gctcattcgt tcaatttgtt agaaaaaatt gggctgattg tagcacagta aggcagaaag
    11701 gagggaatac aagcggtcaa tttccgttag aaatttgcaa atgctaaaa
//
LOCUS       DANLUQ010000072        11635 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.072, whole genome shotgun sequence.
ACCESSION   DANLUQ010000072 DANLUQ010000000
VERSION     DANLUQ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11635
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.072"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..257)
                     /locus_tag="PXB27_001481"
     CDS             complement(<1..257)
                     /locus_tag="PXB27_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346259.1"
                     /translation="MITSNLERSFHGLNIAQHNYKIFLDESGGNIGKLKHTPNDKKGS
                     NFNRTISSHIGVPPVFVLGGIAQISNANLIAPDWRKLLHLQ"
     gene            complement(284..958)
                     /gene="cas4"
                     /locus_tag="PXB27_001482"
     CDS             complement(284..958)
                     /gene="cas4"
                     /locus_tag="PXB27_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HDL5346260.1"
                     /translation="MGDLQNSAEILPLVQPKFGDDKRSISLSALQHYAFCPRQCALIH
                     NEQVWAENYLTAQGQALHERVDSGEPEVRKGIRFERTVHVAAEKLGISGILDLVERDL
                     KTGELKPVEYKRGKPKLDPIDEIQLCAQALCLEEMTEQTINEGALWYMQTRHRLPIIF
                     STELRTKTLATIAEVRSLLDSGTTPPPEYSKRCKACSLIDLCQPKLLERDRSKGYVVG
                     VFGSLL"
     gene            complement(958..1821)
                     /gene="cas7c"
                     /locus_tag="PXB27_001483"
     CDS             complement(958..1821)
                     /gene="cas7c"
                     /locus_tag="PXB27_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HDL5346261.1"
                     /translation="MSIQNRYEFVFFFDVTNGNPNGDPDAGNMPRLDPETSKGLVTDV
                     CLKRKIRNFIEMSYENEAGYEIYVKEKSVLNLQNKRAYEALGIESEAKKLPKEEAKAR
                     EITAWMCKNFFDIRTFGAVMTTEVNSGQVRGPVQLAFAQSIDPIIPLEVSITRMAVTN
                     EKDLEKERTMGRKYIVPYALYRVHGFISAKLAEKTGFSDEDVQKLWQALQLMFEHDRS
                     AARGEMAARKLIVFKHDSELGNQPAHKLFDSVKVERINGEKDTPAKDYDDYCISVQTE
                     GLTGVTVEELL"
     gene            complement(1831..3612)
                     /gene="cas8c"
                     /locus_tag="PXB27_001484"
     CDS             complement(1831..3612)
                     /gene="cas8c"
                     /locus_tag="PXB27_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HDL5346262.1"
                     /translation="MITALTRYYYRLAAQEDRLTGEAKVPSYGFSEEKIGWVLELDSK
                     GNLVNVISNLTEDKKPKPKLMSVPRPEKRTSGIKPNFLWDKTAYVLGIESNPDKATAK
                     DQPINFAEKTFEAFKQYHLDLLADSNDEGLMAIRNFLQQWQPTHFAESICPREILDAN
                     VVFKLVGTSGYIHQREAAQTLWTSLLINQSDNAEKGLCLISGKLSPIQRTHPPIKGVF
                     GGQSSGGSIISFNKESFTSFGKEQGSNAPVSEQAAFAYTTALNYLLRTEKHRLTIGDA
                     STVFWAEADNTTQAEAAEGFFAALMTPPDDEQENQKIFEELQKISKGRPLAEISPELS
                     ENTRFYVLGLAPNAARISVRFWLDTTFGQLAKNLSEHWQDLALEPCPWKTPPSIWRLL
                     LQTASLGKSENISPVLAGEMARAVISGHLYPMSLLSQLVTRIRADGDINGLRVALMKA
                     VLQRKFRKGFIKEGVPMSLNKESDNQAYLLGRLFAVLERIQSQALGELNAGIADRYYG
                     SASAVPFSVFPRLLSGAKHHLSRLRKDKGGMAVNLDKDLGEIIASLPENFPRHLSIDE
                     QGRFAIGYYHQKQSYFAKKETTESIEN"
     gene            complement(3634..4311)
                     /gene="cas5c"
                     /locus_tag="PXB27_001485"
     CDS             complement(3634..4311)
                     /gene="cas5c"
                     /locus_tag="PXB27_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5"
                     /protein_id="HDL5346263.1"
                     /translation="MPNKIRLHIWGDYACFTRPEMKVERVSYDVITPSAARGILAAVH
                     WKPAIRWVIDKIYVLQPIQFESVRRNELGSKISASKISGAMKRKTVADLYTVIEDDRQ
                     QRAATVLKNVGYVIEAHAVLTKMAGEAETVTKHIEMFKRRAKKGQCFQQPCMGVREFA
                     ANFALIDDDEPLPECFLAENERDRDLGWMLHDIDFEHGNQPRFFRAEMKNGVIDVPPF
                     YAEEVKS"
     gene            complement(4553..6304)
                     /locus_tag="PXB27_001486"
                     /pseudo
     CDS             complement(4553..6304)
                     /locus_tag="PXB27_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250669.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
     gene            complement(6294..6755)
                     /locus_tag="PXB27_001487"
     CDS             complement(6294..6755)
                     /locus_tag="PXB27_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346264.1"
                     /translation="MFKPHGSLDWFMAGDVPMTSCIGKVEEPLIITPGVGKYKKGYGQ
                     PFDAHREKANEAIDSASSILCIGYGFNDDHLQTHLTGKIKSGVKTLLLTRGLSENARN
                     VVKEAPNCKALIFDGDKAGTRVVSKQEETFIPNVHWWDVEFFVKEILENGK"
     gene            complement(6914..8050)
                     /locus_tag="PXB27_001488"
     CDS             complement(6914..8050)
                     /locus_tag="PXB27_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL5346265.1"
                     /translation="MLLRKAFKFEIRPNGEQVRKMKQFCGCSRFVFNKALAWQNEQYE
                     QDNHHKFSYTKLANLLPQWKKELVWLKDCHSQVLQQSLKDLESAFKNFFQKRADLPKF
                     KKKGVKESFRFPQGCKIEQKNDRLYLPKIGWVRYRNSREIVGEVKNVTVSMKCGRFFV
                     SIQTEFKQEIPTHKGGEIGIDMGIARFATLSNGEFFEPLNAFKTHKGKLAKLQKQLKN
                     KVKFSKNWQKLKDKIGKLHHKIANCRKDFLHKISTQISKNHAMIYVEDLQVANMSKSA
                     KGTAEVHGKNVKQKSGLNRVILDQSWFEFRRQLDYKTQWLGGFLLAVPPQNTSRTCPC
                     CNYTAKENRQTQENFECVECGYTENADVVGAMNVLTRGQAIVQA"
     gene            8097..8513
                     /gene="tnpA"
                     /locus_tag="PXB27_001489"
     CDS             8097..8513
                     /gene="tnpA"
                     /locus_tag="PXB27_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005720092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDL5346266.1"
                     /translation="MKKETEIRRGRHVVFNLHVHLVFVTKYRREVFTQAILNDLHSIF
                     ATICADFEATLVEFDGEDDHVHLLVEYPPKVAISHLVNSLKGVSSRMIRKKNYPSIRK
                     KLWGNQLWSPSYFAGSCGGAPISIIRQYIEQQQTPD"
     gene            complement(8575..9168)
                     /locus_tag="PXB27_001490"
     CDS             complement(8575..9168)
                     /locus_tag="PXB27_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346267.1"
                     /translation="MREPLLDEDELKKWLQTIFTDNLVIIVGSGLSCAEGLPGMGALA
                     DRLKERMPECLDDIDKVTWNTISDCLDSEGLEGALLKHQANETIEAAIIKITAEYVLS
                     EEQKAINKCIAENQKLKFSYLLPHISASNPKIARVITTNYDRLIEFAAEYENWGIDSM
                     MVGRYWGKHNPDLSRKLQIRDIRVKGKCPKLVYLIVP"
     gene            complement(9299..11587)
                     /locus_tag="PXB27_001491"
     CDS             complement(9299..11587)
                     /locus_tag="PXB27_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HDL5346268.1"
                     /translation="MEEHTIQSFYAHSGKLSDHSDWQTLLSHANNVGLLAAEFAAPFA
                     SQEIARCTGLLHDLGKYSERYQKRLHGGERVNHSTAGAKIAVERWGPLGKMMAFCIAG
                     HHAGLANGSGEGKNRSTLTQRLQEQFGYGHKDLPVLDAIWQQELNLPEKLPLPPLKPS
                     KNEPFFSYAFFIRMLYSCLVDADFLDTEAFYANLEKRPLERGNYPNLTALHQQFNQFI
                     AQFRERIATQTPNTQEAQRKAEVNRLRSEILDYAVSQAKQETGLFSLTVPTGGGKTFT
                     SMAFALEHAKQYGLCRIIYVIPFTSIIEQNAAEFRKAFGELGTEAVLEHHSTFDHDSL
                     PDKSSRDKLKLASENWDTPVVVTTAVQFFESLFADRSSRCRKLHNIVGSVIILDEAQM
                     LPLNLLRPIMAAIDELARNYQCSVVLCTATQPAIHQANGFYKGFENVREIAPNPTALF
                     EKLKRTTVKHIGIQTDVELQSKLADNPQMLIIVNNRRHARALYDSAKQLEGAYHLTTL
                     MCAKHRSQTLTEIRLRLKQNLPCRVIATSLIEAGVDVDFPLVMRAEAGLDSVAQAAGR
                     CNREGKRLSVESNVWVFQPEEQWKAPPELGLLSGCMRSTVQVHADDLLNPKAINHYFQ
                     AVYEAKGKELDKPNILKACHDAGQSLNFPFQKIAEDFRMITSHLIPVIIPFDQEAENL
                     IDRLRYADQVGGILRKLQPYTVQVPEKALAELYNAGRVETINETNFGKQFYCLIGMDL
                     YDEVAGLSWENVDFLESEKLVL"
BASE COUNT         3213 a         2609 c         2214 g         3599 t
ORIGIN      
        1 ttctgcaaat gaaggagttt tctccaatca ggagcaatta aattagcatt actaatttga
       61 gcaatccctc ctaatacaaa tacaggagga accccaatgt gggaagaaat ggttctatta
      121 aaattactac cttttttatc attaggcgta tgttttaact tcccaatatt tccaccactc
      181 tcatcaagaa atattttgta gttatgctgt gctatattta atccgtgaaa tgatctttct
      241 aagtttgaag ttatcacaac aagtccctcc ccaaagtagg taattataat aaagatccaa
      301 acactcccac cacatacccc ttcgatctat ccctttccaa caactttggt tggcagaggt
      361 cgataagcga gcaggctttg cagcgtttgc tgtattctgg aggaggtgtg gttccgctat
      421 ctaagagcga ccgcacttcg gcaatggtgg cgagggtttt ggtgcgtagt tcggtggaaa
      481 aaatgatcgg caaacggtgg cgagtttgca tataccacaa cgccccttcg ttaatcgtct
      541 gctctgtcat ttcttccaaa cataaggctt gggcacagag ttggatttca tcaatcggat
      601 caagtttggg cttgccacgt ttgtattcca ctggttttag ctcgcctgtt tttaggtctc
      661 gctcaaccaa atccaaaatg ccgcttatgc ctaatttttc ggctgcaacg tgaaccgttc
      721 gctcaaagcg aatccctttg cgaacttctg gctcaccact atctacccgc tcgtgcaacg
      781 cttgaccttg tgcggtgaga tagttttctg cccacacctg ttcgttgtgg atcagcgcac
      841 attgacgtgg gcagaaggca tagtgttgca aagcggaaag cgaaattgac cgcttgtcat
      901 cgccaaattt tggctgaaca agcggtaaaa tttccgcaga attttgcaaa tcgcccatta
      961 cagtaattct tcaacggtta cacccgttaa gccttcggtt tgtacgctga tacagtaatc
     1021 gtcatagtct ttcgctggcg tgtctttttc accgttgatt cgctcaactt ttaccgagtc
     1081 aaacagtttg tgtgcaggtt ggttgcctaa ttcgctgtcg tgtttaaata cgatcagttt
     1141 acgagctgcc atttcgcctc gtgcggcaga acggtcgtgt tcaaacataa gttgcaaggc
     1201 ttgccaaagt ttttgtacat cttcatcaga aaagcccgtt ttttcagcga gtttagctga
     1261 aataaagccg tgaacacgat aaagtgcgta aggcacgata tatttacgcc ccatagtccg
     1321 ttctttttca agatcttttt cgttggtcac tgccattcgt gtgatcgata cttctagcgg
     1381 aataatcgga tcgatagatt gagcgaacgc caattgcact gggcctcgca cctgtccgct
     1441 gtttacttcg gtagtcatta ccgccccaaa ggtgcgaata tcgaaaaagt ttttgcacat
     1501 ccaagcggta atttctcggg ctttagcttc ctcttttggt agttttttcg cttccgattc
     1561 aatccctaat gcttcataag cacgtttgtt ttgtaaattc agaacacttt tttctttaac
     1621 ataaatttcg taacccgcct cgttttcata gctcatttca ataaaattgc ggattttgcg
     1681 ttttaggcaa acatcggtga ctaaaccttt actggtttca ggatcaaggc gtggcatatt
     1741 tcctgcatct ggatcaccat ttgggttacc gttggttaca tcaaagaaaa aaacaaattc
     1801 atagcggttt tgaatagaca tagtgtttcc ttagttttca atagactcag tggtttcttt
     1861 tttagcgaaa tagctttgtt tttggtggta ataaccaatc gcaaaacgcc cttgttcatc
     1921 gatacttaaa tggcgaggga aattttccgg caagctggca ataatctcac ctaaatcctt
     1981 atccagatta accgccattc cgcccttatc ttttctcaag cgagataaat ggtgttttgc
     2041 accgcttaat aagcgaggga ataccgaaaa cggcacggca gaagccgaac cataatagcg
     2101 gtcggcaatg ccggcattta attcgcctaa tgcttggctt tggattcttt ctaatactgc
     2161 aaataacctg ccgagcaagt aagcttggtt atcgctttct ttatttaagc tcataggaac
     2221 tccttcttta ataaaacctt ttctaaattt tcgttgtaag actgctttca tcaacgctac
     2281 tcgcaaaccg ttaatatcgc cgtctgctcg gattcgagtc actaactgtg aaagtaaact
     2341 catcggatat aaatgaccgc taataactgc gcgagccatt tcacccgcca agactggaga
     2401 aatattctca cttttaccta atgaagcggt ttgtaataat agtcgccaaa tggaaggtgg
     2461 tgttttccat gggcaaggct caagagcgag atcttgccaa tgctcgctga ggttttttgc
     2521 cagttgccca aaggtggtat ccagccaaaa acgtaccgaa atgcgagctg catttggagc
     2581 aagccccaac acataaaaac gggtgttttc agataattct ggggaaattt ctgcaagcgg
     2641 tcgtcctttg ctgatttttt gcaattcttc aaagattttt tggttttctt gctcatcatc
     2701 tggaggcgtc attaaagcag caaaaaagcc ttcagcagct tcagcttggg tggtattgtc
     2761 tgcttctgcc caaaagacag tgctggcatc accaatagtt aaacgatgtt tctcggtgcg
     2821 taacaaataa tttaaagcag tagtgtaggc aaaggcggct tgttcagaaa caggggcatt
     2881 actaccttgt tccttgccaa atgaagtgaa ggattcttta ttgaaagaga tgattgaacc
     2941 accggaactt tgcccaccaa ataccccttt aataggagga tgagttcgtt gaattggcga
     3001 taatttgcct gaaattaagc ataaaccttt ttcagcatta tcactttgat ttatcaacaa
     3061 acttgtccaa agggtttgtg cagcttctcg ctgatgaata taaccgcttg tgccgactaa
     3121 tttaaacacg acattcgcat caagaatttc tcttgggcag attgattcgg caaaatgtgt
     3181 gggttgccat tgttgcagga aatttcttat cgccattaaa ccttcatcat tggaatctgc
     3241 aagcaaatcc aaatggtact gtttaaatgc ctcaaaggtt ttctcggcga aattgatggg
     3301 ttgatctttg gcggtggctt tatcaggatt actttcaatg cctagcacat aagcggtttt
     3361 atcccacaga aaattgggct taatgccaga cgtgcgtttt tcagggcgag gtacgctcat
     3421 caatttcggt tttggtttct tatcttctgt taaatttgag atcacattga ccaaatttcc
     3481 ctttgaatct aattctagta cccaaccgat tttttcttca ctgaaaccat aagaaggcac
     3541 tttagcttcg cctgttaagc gatcttcttg tgctgcaagg cggtaataat agcgagttaa
     3601 ggcggtaata atagcgagtt aaggcggaga gaatcatgat ttgacctcct ctgcataaaa
     3661 tggtggcaca tcaatcacgc catttttcat ttccgcacgg aagaaacgag gttgattacc
     3721 gtgctcaaaa tcaatgtcgt gcaacatcca gcctaaatcc cgatctcgct cattttctgc
     3781 caaaaaacac tcaggtaacg gttcatcgtc atcaatcaag gcaaaatttg cggcaaattc
     3841 acgcacgccc atacaaggct gctggaaaca ttgccccttt ttcgcacggc gtttaaacat
     3901 ttcaatatgc ttggtgacgg tttccgcctc gcctgccatt ttggtcagca ctgcgtgagc
     3961 ttcgatcaca taaccgacat ttttcagcac agtagcagca cgttgttggc ggtcgtcttc
     4021 aatcacagta tataaatccg ccactgtttt acgcttcatt gcaccactga ttttactggc
     4081 agaaatttta ctgcctaact cattacggcg aacagactca aattgaatcg gttgtaatac
     4141 ataaatttta tcaatcaccc agcgaattgc cggcttccaa tgcaccgcag ccaaaatccc
     4201 ccgtgccgca gacggcgtaa tcacatcata agacacccgt tctaccttca tttcaggacg
     4261 ggtaaaacaa gcataatcac cccaaatatg gagcctgatt ttgtttggca taatagaatg
     4321 ctccttttaa ttatgtaaca cacacctccc cgaagggagg tgaaatttct ttagcttata
     4381 ttataactgc tttggtttca acccacgcac tcagtaaaat gcgaaaaatt ttatggctta
     4441 ttatattaag cggtcggatt tacttagaaa gttgcaaagt tatttgagta tttaaccgct
     4501 tgtatttcaa aaatagtcag aaaaataaca gcattctagg tacaatattt ttctaagaac
     4561 gaacttgtct ccgcaatgcc tctactgcgt ttgacacagc atttggtttg ggctcctggt
     4621 ttgcccaatc agaccagaat tggcgtgtag cactttctgg ggctatatgc ggtttagcaa
     4681 gtttgattcg tgtaggtaat agaatcgaat cgcctagcaa taacgcttcg cctaaatcaa
     4741 gtaacggcaa tatatcagta attccaacca tagattccgg caataaacgc tttacaacac
     4801 tttgatcttg accatttgtt aatcgtaaaa ccaagaaatt attacattga ctcaaaattg
     4861 ttcggcttac atctgaagga cgttggctaa cgaccaacaa agacacgcca tattttctac
     4921 cttctttcgc aatccgctca aaggcttcca atgcgcgttt ttccgctgct tccgctgaat
     4981 ctcgaacagg gagatataaa tgtgcttcat cgcaaataat cgttagtgga tggcgggaat
     5041 ctttatccat ccaaaattga atgttgtata aaaggcgggc aagaactccc gtaacaatcg
     5101 gtaaaacatc cgacgggact tccgaaaaat caataatttt tatccctttt tcgctctcag
     5161 aagcagaaaa cagttttgac aacatatttt ccagccaccc atagctgttg cactctactg
     5221 gcggttggaa cataaaaccg caacgtctgt catctatttt ggctttcatc cttcccataa
     5281 acctagacaa cttactatac caaggacctt gctttgttcc tgaacctccc tctttaggta
     5341 ccatctcaga attatcttta tctaaacgag ctaacaataa ttcaatagaa tacggaattg
     5401 gcgaatcaac agtaaacgtt tccaaaacat catttctttc ttccttattc aatgtttgct
     5461 cttttaatgc gcgaacatgc tcagtaaaac gcgccgcctg attcggagca ttatcatcac
     5521 tgcgatctaa aattaaagcc agcatttatt cccgattgag cagccaatag ggaataaata
     5581 aactgcgttc atctgaacca tccaaatctc ccggtccagc aatacggtat tgctgagcga
     5641 tagcgttttt accttcgcat agagtgctat attcaccgtg catatcgaat acaataagat
     5701 tagcagcttt taaagctgct gcacgttcca atattaatgc aactgaccag cttttaccgg
     5761 aaccagtact acccaaaata gcagcgtgcc gttgaaagaa tttatctccg tttgctatag
     5821 ctacagccgt ttcatcagcc ataaaatgac ctaaagtcag ccgttcattt tcttttacgt
     5881 tagcggacaa aagattcata aataattgca gattgctgcc actaatatga aagcaatctc
     5941 tatcaatttg cggaaaacaa tccgctcccc gcttaaaagt gttgctcaaa ctaccatcaa
     6001 cagctttata agtaccgatt aatactgcct tgataaagtc atcctgactt tcacccagag
     6061 gaatttcacc ctcattagat atttcttcta gcagcaattt ttcactgata gaacgcgtta
     6121 cccgttcgat aatgccgata aggtactcac tagccgttct gccttgaatc gcaatcagat
     6181 tacccacagc aatactagtc atttgttgat gctcttcaac agaaatcaat acacggctgg
     6241 tatctactgt tacaaccctg cccaaacggg cattgtcttt aaaattcaat gtttcatttg
     6301 ccattctcta aaatctcttt tacaaaaaac tcaacgtccc accaatgaac attaggaata
     6361 aaagtttctt cttgcttgct aaccaccctt gttccagcct tatctccatc aaaaatcaga
     6421 gccttacaat tcggagcttc cttcaccacg tttctggcat tttctgaaag tcctcttgtc
     6481 agaagtagtg ttttcacacc acttttaatt ttgcctgtca aatgtgtttg aaggtggtca
     6541 tcattaaaac catagccgat acataaaatt gaagaagcag aatcaatagc ttcatttgct
     6601 ttttctcgat gagcatcaaa tggttgtcca tagccttttt tatacttccc aacaccaggc
     6661 gtaataatta aaggctcttc cactttacct atacaacttg tcattggaac gtctccagcc
     6721 ataaaccaat ctaaactacc atgcggttta aagattttga tatgatctcg ttgacatcct
     6781 cccctgccta aaggcagggg attcctacta gctcccacag caagctgtgg gctactctcg
     6841 gtgggtttct actgctgatc tctattgaga ttcacttcat agacgcttcg ggtatgtcct
     6901 accctgatat ttattatgct tggactattg cttgcccacg tgtaagaaca ttcattgctc
     6961 ccactacatc ggcattttcc gtatagccac attctacgca ttcaaaattt tcctgtgttt
     7021 ggcgattttc ttttgctgta tagttgcaac aagggcaagt acgactggta ttttgtggag
     7081 gaactgctag caaaaatccg cctaaccatt gcgttttgta gtccagttgt cgtctaaatt
     7141 caaaccaaga ctggtctaat atcactcggt ttaagcctga tttctgtttc acatttttgc
     7201 cgtgtacttc cgctgtgcct ttggctgatt tggacatatt cgccacttgc aaatcttcaa
     7261 catagatcat cgcgtggttt ttgctgattt gcgttgagat tttatgcagg aaatcttttc
     7321 ggcagttggc gattttgtga tgtaacttgc cgattttatc tttcagtttt tgccagtttt
     7381 tactgaactt cactttattt tttagctgtt tttgcagttt tgccaatttt cctttgtggg
     7441 ttttaaacgc atttaacggc tcaaaaaatt ctccattgga aagggttgca aaacgagcaa
     7501 tgcccatatc aatgccgatt tctccgcctt tatgcgtggg aatttcctgt ttaaattccg
     7561 tttgaatgct cacaaaaaag cgtccgcatt tcattgaaac ggtgacattt tttacttccc
     7621 caacaatttc acgactgttg cgataacgca cccaaccgat tttcggcaga tacaagcggt
     7681 cgtttttctg ctcgattttg caaccttgcg gaaaacggaa actctctttt acgccttttt
     7741 tcttgaattt gggcaaatcc gcacgctttt gaaagaagtt tttaaacgca ctttcaaggt
     7801 cttttaacga ctgttgaagc acttgtgaat ggcaatcttt aagccatacc aattcttttt
     7861 tccattgcgg aagcaagttg gcgagcttgg tataactgaa tttgtgatgg ttatcttgtt
     7921 cgtactgctc attttgccac gccaatgcct tgttaaacac aaagcgagaa cagccgcaaa
     7981 actgcttcat tttgcgaact tgttccccat ttggtctgat ttcaaattta aaggctttac
     8041 gtagtaacat tgcacgaatt agaatacaaa aattgtgctt attctacact tggcctatga
     8101 aaaaagaaac agagattaga cgtggtcgcc acgttgtttt taacttacac gttcatttag
     8161 ttttcgtgac taaatatcgc agggaagttt ttactcaagc aattttgaac gacttacact
     8221 caatttttgc aacgatttgt gctgattttg aagccacatt agtggaattt gatggtgaag
     8281 atgatcacgt tcatctactt gtggaatatc cgcctaaagt cgctatttct catcttgtaa
     8341 acagtttaaa aggcgtttcg agccgaatga ttagaaagaa aaactatccg agcattcgca
     8401 aaaaattatg gggaaatcaa ctttggtcac cgtcttactt tgctggtagt tgtggtggtg
     8461 caccaatatc gattattcgc caatatatcg agcaacaaca aacacctgat tagttagaaa
     8521 tttgaaacgg ctttgccgtt tataccttat atccacgccc taaaggacgt ggttttacgg
     8581 cacgattaga taaaccaatt ttggacattt accttttact ctaatgtctc ttatctgcaa
     8641 tttcctactc aaatcaggat tatgttttcc ccaataacga ccgaccatca ttgaatctat
     8701 tccccaattt tcatactctg ccgcaaactc aatcaatcta tcataattag tagtaattac
     8761 acgggcaatt ttaggatttg aagctgaaat atggggaagc aaataagaaa atttgagctt
     8821 ctgattctca gcaatacatt tgtttatcgc tttttgctct tcggagagta catactcggc
     8881 tgttatttta ataatagcgg cttctatcgt ctcatttgct tgatgcttca acaatgcacc
     8941 ttccaagcct tcagaatcca gacaatcaga aatggtattc catgttacct tatctatatc
     9001 atcaagacat tcaggcattc gttcttttaa tctatcagct aatgctccca ttcccggcaa
     9061 accttcggca caggacaaac ccgaaccgac aataatgacc aaattgtcag taaaaatagt
     9121 ttgcaaccat ttttttaatt catcttcatc taataatggt tctctcattt tacgtttccc
     9181 ttccaaatat ctattcttac aaactagctg tttactacga gacgtcatag gctatttggt
     9241 atattaaagt atatctatgc aaaattaaac ttataaagaa gttaaaataa acattcatct
     9301 ataaaaccaa tttctccgac tcaagaaaat ccacattctc ccaactcaac cccgccactt
     9361 catcatacaa atccattcca attaagcagt aaaactgttt gccgaagttg gtttcattaa
     9421 tggtttcaac tctgccagcg ttatagagtt cggctaatgc tttttccggt acttgcacag
     9481 tgtaaggctg gagttttctt aaaataccgc ctacttgatc cgcatagcgt aaacggtcaa
     9541 taaggttttc ggcttcttgg tcgaaaggga taatcaccgg aattaaatgg cttgtgatca
     9601 ttcggaaatc ttcagctatt ttttggaatg ggaaattaag gctttgccca gcgtcgtgac
     9661 acgcctttaa aatatttggc ttatccagct cttttccttt agcctcataa accgcttgga
     9721 aataatgatt gattgctttc ggattaagta gatcatctgc gtgaacctga accgttgaac
     9781 gcatacagcc agaaagtaag cctagctctg gtggtgcttt ccactgttct tcaggttgga
     9841 aaacccaaac attgctttct acagataaac gtttcccctc tcggttgcaa cgccctgctg
     9901 cttgagccac tgaatctagt cctgcttcag ctcgcataac cagtgggaaa tcgacatcaa
     9961 caccggcttc aattaaagaa gtggcaatca ctcggcaagg taggttttgt tttaggcgaa
    10021 gacgaatttc agtcagcgtt tgtgagcggt gtttagcaca cattagcgtg gtgaggtgat
    10081 aagctccctc taactgtttc gctgaatcat ataacgctcg ggcgtgacgg cggttattca
    10141 cgattatcag catttgtggg ttatctgcga gtttgctttg caactccaca tctgtttgaa
    10201 taccgatatg ctttaccgta gtacgtttca atttttcaaa aagtgcggtt ggattagggg
    10261 caatttctcg cacattttca aatcctttat aaaaaccgtt cgcttgatga atagcgggtt
    10321 gtgtcgctgt gcaaagcact accgaacatt gataattgcg agctaattca tcaatcgcag
    10381 ccataattgg gcgtaataaa ttcaacggta gcatttgggc ttcgtccaaa ataatcacgc
    10441 taccgacgat attatgcaat ttgcgacagc gtgaagaacg gtcggcaaac aaactttcaa
    10501 agaattgcac tgcagttgta accacaacag gagtatccca attttctgat gccaatttca
    10561 gcttatctcg gcttgattta tcaggtaggc tatcgtgatc aaaagtgctg tgatgttcca
    10621 ataccgcctc tgttcctaat tcgccaaaag ctttacgaaa ttccgcagca ttttgttcaa
    10681 taatgctggt aaacggaata acataaataa tgcggcataa accatactgt ttagcatgct
    10741 caagggcaaa cgccattgaa gtaaaggttt taccaccgcc cgttggcaca gtcagagaaa
    10801 acagacctgt ttcctgtttt gcttgagata cggcataatc caaaatttca ctccgcaagc
    10861 ggtttacttc cgctttacgt tgtgcttcct gcgtattagg cgtttgagtg gcaattcgct
    10921 ccctaaactg tgcaataaat tgattaaatt gctgatggag tgcggttaaa ttggggtaat
    10981 ttcctcgttc aagcggtctt ttttcgagat tggcataaaa agcttctgta tcaagaaagt
    11041 cggcatcgac aaggcaagaa tagagcattc ggataaaaaa ggcgtaagaa aagaaaggtt
    11101 cgtttttaga aggctttaac ggcggtaagg gcaatttttc aggcaaatta agttcttgct
    11161 gccaaatagc gtcaagtacc ggtagatctt tatgaccata gccaaattgc tcttgcaaac
    11221 gttgtgtaag tgtagaacga tttttccctt ctccagaacc atttgctaac ccagcgtggt
    11281 ggcctgcgat acaaaacgcc atcattttgc ccaaaggccc ccaacgttcc actgcaattt
    11341 ttgcaccagc agtagagtga tttacccgtt ctccgccatg taaacgcttt tggtaacgtt
    11401 cactatattt gcctaaatcg tgcaatagcc ccgtgcagcg agcaatttct tgtgaagcaa
    11461 acggcgcagc aaattcggcg gcaagcaagc caacattatt ggcgtggctg agtaaagttt
    11521 gccaatctga atgatctgaa agcttccccg aatgggcata aaaagactga atagtatgtt
    11581 cttccatttt tataccttca atatttcctt cctaacacaa gcaatactac ctcta
//
LOCUS       DANLUQ010000073        11619 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.073, whole genome shotgun sequence.
ACCESSION   DANLUQ010000073 DANLUQ010000000
VERSION     DANLUQ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11619
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.073"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(30..4490)
                     /gene="mukB"
                     /locus_tag="PXB27_001492"
     CDS             complement(30..4490)
                     /gene="mukB"
                     /locus_tag="PXB27_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HDL5346269.1"
                     /translation="MIDTTELQNNPQIAPLARPNDVQRGKFLSLTLINWNGFFARTFD
                     LDDLVTTLSGGNGAGKSTTMAGFVTALIPDLTLLNFRNTTEAGSTAGSRDKGLYGKLK
                     AGVCYAVLESFNSRGQRIITGVRLQQIVGRDKKVDIRSFSLQNIPQEQSIISLLTEQV
                     GNKARVLALNELKDQFENSEVQFKQYHSITDYHSFMFDLGVIPKRLRSAADRSKFYKL
                     IEASLYGGISSVITKSLRDYLLPENTGVRQAFQDMESALRENRMTLEAIKVTQSDRDM
                     FKRLITESTNYVSADYMRNANERRGNVQQALEQRKEWYAAKSKILLEQQRFVEFSRES
                     ADIAEAELALEADYNSANDHLNLVMNALRHQEKIERYQDEVEELNIKLEEQQEALEEI
                     AEIAENAQARADEADDHVEELRSQMADYQQALDAQQTRALQYQQAVNALEKAKQLTGL
                     VNLDLNNIEDYHAEFVAQAEDLTDQVFELEQRLSVSDMAKTQFEKAFELVCKISGEID
                     RLQAWEEARALLSAFPEQKMQAQQAVSLRQKLNDLEQRLQQQQNAQRLVAEFNQKSQQ
                     QLNSAEELDAYFEEQQARLEDLEAELSDFVEQRSTQRQQREQLNQQYQQLAKNAPAWH
                     TAQSALTRLEEQCGEKFEASQSVMQFMQNMLSKEREATLARDELARKEQMLDEQINRL
                     SQPDGSEDARLNQLAERFGGVLLSELYDDVSIEDAPYFSALYGEARHAIVVRDLEAVK
                     TQLEKLDDCPSDLYLIEGDPSAFDDAVFSAEELGEGVVVKVSDRQWRYSKFPEVPLFG
                     RAAREKHLATLKAERDETAEKHAERAFDVQKCQRLHQHLSQFIGTHLNLAFQENPEML
                     MQEIAAERAEIERELAQSAGNEQQLRHQLESVKAQLQMLNKILPLANLLADETLEARA
                     EECREQLELAEEDEMFIRQFGATLSQLEPIAAALKSDPALFEQLEADYRRSVAEQKVL
                     QQKVFSLADVMNRRLHFSYQETVGTEGSALTEQLRQRLENAQREREQARDQLRQAQAQ
                     LSQYNQVLTGLRSSYEAKNQMLQELIREIDDLGVRGDSGAEERARFRRDELQQRLSQQ
                     RSRKGYLDKQIAVIEAEMDNLNRTLRKAERDYHTQRELVVQAKASWCIVQRLSRNSDV
                     EKRLNRRELAYQSAEELRSISDKALGALRTAVADNEYLRDSLRASEDSRKPENKVAFF
                     IAVYQHLRERIRQDIIKTDDPIDAIEQMEIELSRLTNELTGREKKLAISAESVANILR
                     KTIQREQQRILQLNQGLQNIAFGQVKGVRLVVNIRDTHAILLNALSNNREEHKDLFDS
                     QKLSFSEALAMLYKRVNPHIEMGQRTPQTIGEELLDYRNYLDLEVETFRGADGWMRAE
                     SSALSTGEAIGTGMSILLMVVQSWEEEARRMRAKDILPARLLFLDEAARLDATSINTL
                     FELCERLDMQLLIAAPENISPERGTTYKLVRKITNNQEYVHVVGLKGFAK"
     gene            complement(4666..5382)
                     /gene="mukE"
                     /locus_tag="PXB27_001493"
     CDS             complement(4666..5382)
                     /gene="mukE"
                     /locus_tag="PXB27_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HDL5346270.1"
                     /translation="MTENIQDFISPKLALAIANPIFPELDSQLRAGRHINTEQLDQHV
                     FLMDFQAELESFYRRYHVELIRAPEGFFYLRPKASTLIARSAMSEMEMLVGKVLCYLY
                     LSPERLAQQGIFSQDDVYEELLSLADESKLLKAVNPRSTGSDLDRAKLAEKAGGALRR
                     LARIGIITRVGEQNSKKFVISESVFRFGADVRTGDDPREAQLRLIRDGEATTPALLNE
                     QAVEIDENIADEAPFDGENE"
     gene            complement(5431..6774)
                     /gene="mukF"
                     /locus_tag="PXB27_001494"
     CDS             complement(5431..6774)
                     /gene="mukF"
                     /locus_tag="PXB27_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HDL5346271.1"
                     /translation="MQNELAQTIPELISWTKDREFSLSLSSDRLAFLLAISIYNQEQT
                     DGELLESDLIDLFRYVSNSFEQSDATFTQRANNAINDLVRQRLLNRFSSEFTEGLAIY
                     RVTPLGVGISNYYVRQREFSTLRLSIQLSIVADEIQRASVAAEEGIEQAQDERFWRNN
                     VFAPLKYSVAEIFDSIDLSQRIMDENQHQIRERIAELLSQNWHEAIMSCEQLLDETSG
                     NLRELQDTLNAAGDKLQAQLLRIQSCLMARNDLDFIDQLIVNLQNKLDRIISWGQQAI
                     DLWIGYDRHVHKFIRTAIDMDKNRVFGQRLRQSIQDYYNSPWLLYTAKADSLLDLRDD
                     EAMLNEAESVGELPSELEYESLSDVQEQIVSAMQAHLAPFRAEGKPIDLGVILREQLA
                     RFPESRHFDVARIIVDQAVKLGMASQDSQAVYPQWQPINEQGAEVQANVIDQYNK"
     gene            complement(7039..7926)
                     /gene="galU"
                     /locus_tag="PXB27_001495"
     CDS             complement(7039..7926)
                     /gene="galU"
                     /locus_tag="PXB27_001495"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDL5346272.1"
                     /translation="MKVIIPVAGLGTRMLPATKAIPKEMLILADKPLIQYIVNECVAA
                     GFKEIVLVTHSSKNAIENHFDTSFELETMLEKRVKRQLLDDVRSIVPKDVTLIHVRQG
                     QAKGLGHAVLCGRTVVGDEPFAVVLPDVLLGEFTANQKTENLAAMVKRFQETGYSQIM
                     VAPVPMENVSSYGVADCHGVDIPLGGAAPIAKMVEKPSIEEAPSNLAVVGRYVFSSNI
                     WNLLEKTPVGVGDEIQLTDAIDMLIHQETVEAFHMTGRSFDCGYKVGYMKAFVEYSLN
                     HDKCGKEFKAFIKELAKSL"
     gene            complement(8062..8247)
                     /gene="csrA"
                     /locus_tag="PXB27_001496"
     CDS             complement(8062..8247)
                     /gene="csrA"
                     /locus_tag="PXB27_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HDL5346273.1"
                     /translation="MLILTRKIGEVLLVGDDVEITVLSIRGNQVKLGVNAPKEISVHR
                     REIYERIKASVEEENAE"
     gene            complement(8337..10964)
                     /gene="alaS"
                     /locus_tag="PXB27_001497"
     CDS             complement(8337..10964)
                     /gene="alaS"
                     /locus_tag="PXB27_001497"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDL5346274.1"
                     /translation="MKTTSDIRQSFLEFFQTKGHTIVPSSSLVPENDPTLLFTNAGMN
                     QFKDVFLGLEKRPYSRATTAQRCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYF
                     KQDAIKFGWEFLTSPQWLGLPKEKLYVTVYETDDEAYDIWHKEVGVPSEHIIRIGDNK
                     GAPYASDNFWAMGDTGPCGPCTEIFYDHGPEHWGGLPGSPEEDGDRYIEVWNIVFMQF
                     NRLADGTMEKLPKPSVDTGMGLERMTAVMQHVNSNYETDIFQTLIKEVATLLNVKDLD
                     NKSLRVVADHIRACSYLIADGVLPSNEGRGYVLRRIIRRAVRHGNILGAKEAFFYKLV
                     PTLATVMGQAGEVLTQKQAHIQKTLKAEEEQFARTLERGLNLLEEALAKVENNTLSGE
                     VAFKLYDTYGFPLDLTADVCRERDIKIDEDGFNAEMTAQRERAKASSSFGTDYNNVIK
                     VDSKTCFSGYTETSLNDATVIGLFSNGKSVESIQSGENAVVILDKTPFYAEMGGQVGD
                     SGQISAEICNFEVVDTQKYGQVFGHIGQLTSGSLSVGDKVNTIVDRERRHAITLNHSA
                     THLLHSALRQVLGDHVAQKGSLVSENILRFDISQPEAITKSQLEEVERIVNAKVRENI
                     QVIIEEMDIESAKAKGAMALFGEKYGEVVRVVEMSDFSIELCGGTHVKQTGDIGLFKI
                     TSEGAVAAGVRRIEAVTGENAIAWLHTLQQAVQQSAELLKADSHSFVEKIVQLQEKAK
                     RTEKELQQLKDKLAAQAGSDLAKQATQINGVNVVIQQLENVEPKALRTMVDDLKNQLG
                     SAVVVFATTAEDKVNLIVGVTKDLTDKVNAGQLVGAMAEKVGGKGGGRPDMAMAGGSE
                     PQHLLQALSLAQEWITAKL"
     gene            complement(11159..11584)
                     /locus_tag="PXB27_001498"
     CDS             complement(11159..11584)
                     /locus_tag="PXB27_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL5346275.1"
                     /translation="MYKHILVAVDLSEESLVLVRKGAALAEKCGAKLSLIHVDVNFSD
                     LYTGLIDINMSSVQYSVVEETNTALAELASKVNYPVSECLNGTGDFSQVLEEAVEKHN
                     VDLLITGHHQDFWSKFMSSTRRVMNNITVDMLVVPLADE"
BASE COUNT         3148 a         2695 c         2203 g         3573 t
ORIGIN      
        1 tatacatatt tagattaaag tgctaagttt tatttagcaa agccttttaa accaacaacg
       61 tgaacatact cttggttatt ggtgatttta cgcaccaatt tataagttgt gcctcgttca
      121 gggctaatat tttcaggggc tgcaattaat aactgcatat ccaaacgttc gcagagttca
      181 aatagcgtat taattgaggt agcatctaat cgagccgctt catctaagaa caataagcga
      241 gccggtagaa tatctttggc acgcatacgg cgagcttctt cttcccagct ttgaactacc
      301 attaagagaa tcgacatacc tgtaccgatc gcctcaccgg tcgataatgc actactttct
      361 gcacgcatcc agccgtctgc accacggaag gtttccactt ctaaatctaa gtagttacgg
      421 taatccaata actcttcacc aatggtctgt ggtgtacgtt gccccatctc aatatgaggg
      481 ttcacccgtt tatacaacat cgctaaggct tcggagaagc tcagtttttg gctgtcgaat
      541 aaatctttgt gttcctcacg attattggat aacgcattga gtaaaatagc gtgcgtatct
      601 cggatattca ccaccaaacg cacccctttt acctgaccga atgcaatatt ttgtaaacct
      661 tggttcagtt gcaaaatgcg ttgttgttca cgctgaatgg ttttacgtaa aatatttgcc
      721 acactttccg cactgatcgc cagttttttc tcacgaccgg ttaattcatt ggttaagcgt
      781 gaaagctcga tttccatctg ctcaatcgca tcaatcggat catcggtttt gataatgtct
      841 tggcgaatcc gctcacgcaa atgctgataa accgcaataa agaacgccac tttgttttcc
      901 ggcttgcgac tatcttcaga agcccgcaag ctatcacgca ggtattcatt atccgccacg
      961 gcggtgcgta atgccccgag agctttatcc gaaatcgaac gtaattcttc agccgattga
     1021 taagccaatt cacggcgatt taaacgtttt tccacatcgc tgttacgaga taaacgttgc
     1081 acaatacacc agctggcttt tgcctgcacc accaattcac gttgtgtatg ataatcacgt
     1141 tcggctttac gtaaggtgcg gttaaggtta tccatttccg cctcgatcac cgcaatttgt
     1201 ttatctaaat aacctttgcg ggaacgttgc tggcttaaac gttgttgcaa ttcatcacgg
     1261 cggaatctgg ctcgctcttc cgctccgcta tcaccacgca cgcccaaatc gtcaatttca
     1321 cgaattaact cttgtagcat ttggtttttc gcctcataag aactgcgtaa gccggtcaat
     1381 acttggttat attgtgaaag ttgagcttga gcttgacgca gctgatcacg agcctgttca
     1441 cgctcacgtt gtgcattttc caaacgttgg cgtagctgtt cggttaatgc agagccttct
     1501 gtacccaccg tttcttgata gctaaaatgg aggcggcgat tcattacatc agccaagctg
     1561 aacacttttt gctgtaacac tttttgctcg gcaactgaac ggcggtaatc ggcttccaac
     1621 tgttcaaata aagccggatc actttttaat gcggctgcaa tcggctcaag ttggcttaat
     1681 gttgctccaa attggcggat aaacatttca tcttcttctg ccaattctaa ttgctcacga
     1741 cattcctccg ctcgtgcttc cagcgtttca tcggctaata aattcgcaag cggtagaatt
     1801 ttattcaaca tttgcaactg ggcttttacg ctttctaatt ggtgacgtaa ttgctgttcg
     1861 ttaccggctg attgtgccaa ttcacgttca atttctgccc gttctgccgc aatctcttgc
     1921 attaacattt cagggttttc ttggaacgct aaatttaagt gcgtgccgat aaattggctt
     1981 aagtgttgat gtaaacgctg gcatttctgc acatcaaagg cacgttcagc gtgtttttct
     2041 gcggtttcat cacgctctgc ttttaaagtc gctaaatgtt tttcacgagc agcacgtccg
     2101 aataacggca cttcagggaa tttagaataa cgccattggc gatcggagac tttcaccacc
     2161 acgccttcgc ctaattcctc ggcactaaat accgcatcgt caaaggcgga cggatcacct
     2221 tcaattaaat aaagatcact tgggcaatcg tctaatttct ctagctgtgt tttaaccgcc
     2281 tctaaatcac gcactacaat agcgtggcga gcctcgccgt ataaggcgga gaaataaggc
     2341 gcatcttcaa ttgatacatc atcatataac tcagagagta acactccacc aaaacgttct
     2401 gccaactggt ttaagcgggc gtcttccgag ccgtccggct ggcttaagcg attaatttgc
     2461 tcatcaagca tttgttcttt acgagccaat tcatcacgag caagggtggc ttcacgctct
     2521 ttgctcagca tattttgcat aaactgcatc actgattggc tggcttcaaa cttctcgcca
     2581 cattgctctt ctaaacgagt cagagccgat tgtgccgtat gccaagccgg tgcattcttc
     2641 gctaattgct gatattgttg attaagctgt tcacgttgtt ggcgttgggt tgaacgttgt
     2701 tctacaaagt ccgaaagctc tgcctctaaa tcttctaggc gagcttgctg ctcttcaaaa
     2761 taggcgtcta attcttccgc tgaattgagt tgttgttgcg atttttggtt aaattccgcc
     2821 actaaacgct gggcattttg ctgttgttgc aaacgctgtt ctaaatcgtt gagtttttga
     2881 cgtaaactca ccgcttgctg agcttgcatt ttttgctcag ggaaggcaga aagtaacgca
     2941 cgagcctctt cccacgcttg caagcggtca atttcaccgg aaattttgca aaccaattca
     3001 aacgcttttt caaattgggt tttcgccata tccgacacgc ttaaacgttg ttccagctca
     3061 aatacttgat cggtaagatc ttcggcttga gccacaaatt ctgcgtggta atcttcaata
     3121 ttatttaaat ctaaattcac taagccggtt agctgtttgg ctttttctaa cgcattcacc
     3181 gcttgttggt attgcaatgc acgagtttgc tgtgcatcta aggcttgttg gtaatccgcc
     3241 atttgagaac gcaattcttc aacgtgatca tctgcctcat ccgcacgagc ctgagcattt
     3301 tcggcaattt ccgcaatttc ttccaatgcc tcttgctgtt cttccagctt aatattcagc
     3361 tcttcaactt cgtcttggta gcgttcgatt ttctcttggt ggcgtaacgc attcatcact
     3421 aaatttagat gatcgttcgc actattgtaa tccgcctcta acgccagctc tgcttcggca
     3481 atatccgcac tttcacggct aaattccaca aagcgttgct gttcgagcaa aattttggat
     3541 tttgccgcat accactcttt gcgttgctct aaggcttgtt gtacattgcc acggcgttca
     3601 ttcgcattac gcatataatc cgccgacaca taattggtcg attcggtaat taagcgtttg
     3661 aacatatccc gatccgattg cgtgacctta atcgcctcta aggtcatacg gttttcacgc
     3721 agagcggatt ccatatcttg gaacgcttgg cgaacgcccg tattttccgg caacaagtaa
     3781 tcacgcaagg atttggtaat cacacttgaa ataccaccat aaagcgaggc ttcgattaat
     3841 ttgtagaatt tgctacggtc ggctgccgaa cgaaggcgtt ttgggattac gcccaaatcg
     3901 aacataaaac tgtggtaatc agtaatcgaa tggtactgtt tgaattggac ttcgctgttc
     3961 tcaaattgat ctttgagttc atttaacgcc agcactcgag ccttattccc cacttgttcg
     4021 gtcagtaggc tgataatgct ttgctcttga ggaatattct ggagcgagaa cgaacgaata
     4081 tcgacttttt tatcccgtcc gacaatttgt tgcaaacgca cgccggtaat aatccgttgc
     4141 ccacgggaat tgaatgactc taacaccgca tagcaaacac ccgcttttaa cttaccgtat
     4201 aagcctttat cacgagaacc ggcggttgag cctgcttcag tcgtattacg gaaattaagc
     4261 aaggttaaat ctgggatcaa ggcggtcaca aaccctgcca ttgtggtgga cttacctgcc
     4321 ccgttaccgc ctgaaagcgt ggtcactaag tcatctaaat caaaggttcg agcaaaaaaa
     4381 ccgttccagt taatcagcgt taaagagaga aatttaccgc gttgtacatc attcgggcgt
     4441 gcaagcggtg caatttgagg attattttgc aattctgtgg tatcaatcat tatgttttct
     4501 cactaattgt cctagcacgc taaagcgtac taggttaggt ataggtgagt agctccgcta
     4561 ctctaatcgc aacctcagcc ttggagggct gctttatgct taacctagtt cggctttacc
     4621 gtgtctaggg cagactcatt aaatgctgaa tctattcgaa tgaaatcact cattttctcc
     4681 gtcaaagggg gcttcatctg caatattttc atctatttca accgcttgtt cgtttaacaa
     4741 cgcaggggtt gtcgcttcac catcacgaat taagcgaagc tgtgcctctc ttgggtcatc
     4801 accggtacgc acatcagccc caaaacggaa gaccgattct gaaatcacaa attttttgct
     4861 gttttgctcg cctactcggg taataatgcc gatacgggct aatctgcgta acgctccgcc
     4921 ggctttttct gccagcttgg cacggtctaa atcggaaccg gtcgaacgtg ggtttaccgc
     4981 ttttaataat ttgctttcat cggcaagact gagtaattct tcatacacat catcttggct
     5041 gaaaatcccc tgctgagcca aacgttccgg acttaaatag aggtagcaca acaccttacc
     5101 cactaacatt tccatttctg acattgccga acgggcaatt agtgtcgagg ctttcgggcg
     5161 taggtagaaa aagccttccg gtgctcgaat caactcaacg tgataacggc ggtaaaaact
     5221 ctctagttcc gcttgaaaat ccatcaaaaa gacgtgttgg tcaagctgct cggtattgat
     5281 atggcgaccg gctcgtaatt ggctgtctag ctccggaaaa atcgggttgg caatcgcaag
     5341 tgccagtttc ggggaaatga aatcttggat attctctgtc attgtcattc tcttcaattt
     5401 tgcaaaattc tgggtaaaaa cgaccgcttg ttatttattg tattggtcga taacattcgc
     5461 ctgcacttcc gccccttgct cgttaatcgg ctgccattgt gggtaaacgg cttggctatc
     5521 ttggcttgcc atacctaact taacagcttg atcgacaata atacgagcca cgtcaaaatg
     5581 gcgggattcc gggaatcggg caagctgttc acgcaagata acgcctaaat ctatcggttt
     5641 accttcagca cggaacggcg ctaaatgagc ttgcattgcc gaaacaattt gctcttgcac
     5701 atctgaaagc gactcatact ctaactcact tggcaactcg cccaccgatt ctgcctcatt
     5761 taacattgct tcatcatcac gcaagtccag taacgaatcg gcttttgcag tataaagcaa
     5821 ccaagggctg ttgtaataat cttgaatcga ttggcgtaac cgttgcccga atacacggtt
     5881 tttgtccata tcaatcgctg tacgaataaa tttatgcaca tggcggtcat agccgatcca
     5941 aagatcgata gcttgttgtc cccagctgat aatacggtct aacttatttt gtagattcac
     6001 aatcagctga tcgataaaat ccaaatcatt acgagccatc aggcaacttt gaatccgcaa
     6061 taattgagct tgcagtttat caccagcggc atttaaggta tcttgcaact cacgcaaatt
     6121 ccccgaggtt tcgtctaaca attgctcaca actcataatt gcttcgtgcc agttttggct
     6181 aagcagttcg gctattcgtt cacggatttg gtgctgattt tcgtccataa tccgttgtga
     6241 aagatcgatg ctgtcaaaga tttccgccac cgaatatttc aatggggcaa acacattatt
     6301 tcgccaaaaa cgttcatctt gtgcctgctc tattccctct tccgctgcta ctgaagctcg
     6361 ctgaatttca tctgccacaa tcgaaagttg aatcgataaa cgcagggtag aaaattcccg
     6421 ctggcgaacg taataatttg aaattcccac ccctaacggc gtaacacgat aaatcgccaa
     6481 accttccgta aattcgctac taaaacggtt taacaaccgt tgtctgacta aatcattaat
     6541 cgcattattg gcacgctggg taaaagtggc atcagattgc tcaaacgaat ttgaaacata
     6601 acggaaaaga tcaataagat cactttctaa caattcacca tctgtctgtt cttggttata
     6661 aatagagata gcaagtagaa acgctaagcg gtcggaagaa agcgaaagtg aaaattcacg
     6721 atctttcgtc caagagatga gttctggaat agtctgtgcc aattcgttct gcatacgggc
     6781 ttaatcttta atttctatac aaaaatggta tttattgtaa ctgaatgtaa tcaaaatggg
     6841 aatggataaa acgcagacaa gagaaatgaa caatataagc ggtcaaatat gggtgatttt
     6901 ttgcaaaaag tattttaaag gtaaatgaat ccgaaaattt attatcacta cgctaattta
     6961 tgataccata aatagaaaag actggatcca atagaatatc agacacagcc ccttagatca
     7021 tagtctaaac tctaaggctt agagtgattt agctaactct ttaataaatg ctttaaattc
     7081 ttttccgcat ttgtcatgat ttaaactata ttctacaaat gctttcatat agccgacttt
     7141 ataaccacaa tcaaatgagc gccctgtcat atgaaaagct tcaaccgttt cttggtgaat
     7201 caacatatca attgcatccg tcagctggat ttcatcacct acgcctactg gcgttttttc
     7261 taataaattc caaatatttg aggaaaatac ataacgtcct actaccgcta aattagaagg
     7321 cgcctcttca atacttggtt tttcaaccat tttcgcaata ggcgctgccc ccccgagagg
     7381 gatatctaca ccatggcagt cagcaacccc atagctactt acattttcca ttggaaccgg
     7441 cgcaaccata atttggctat atccggtttc ttggaaacgc ttcaccatcg cagctaaatt
     7501 ttccgttttt tggtttgctg taaattcacc aagtaataca tctggtaata caaccgcaaa
     7561 cggttcatcg ccaaccactg ttcgtccaca tagaaccgca tgacctaaac ctttggcttg
     7621 cccttggcga acgtgaatta aggtaacatc ttttggtaca atagaacgta catcatctaa
     7681 taattgacgt tttactcgtt tctccagcat cgtttccaat tcaaaagagg tatcaaaatg
     7741 gttttcgata gcattctttg aagagtgagt aaccaataca atttctttaa aacctgcagc
     7801 cacacattca tttacaatgt attgaataag aggtttatct gcaaggataa gcatttcttt
     7861 tggaatagct ttggttgcgg gtaacatgcg cgttcccaat ccagcaacag gaataatgac
     7921 tttcatttgg aatcctttga tgacttaaca tattagatat aactgaaata aaccgcttag
     7981 acataacagg cggtcaattt tcagaaaatt ttgcattgcc tgcttcgctg ttatcatcta
     8041 gcagctttac agttaaaatg cttattccgc attttcctct tctactgagg cttttattct
     8101 ttcataaatc tctcggcggt gtacagagat ttcttttggt gcattcacac ctaatttaac
     8161 ttgatttcct cggatgctga gtaccgtaat ctcaacatca tctcctacga gtaaaacttc
     8221 cccaatctta cgagttagaa taagcataat tgtctcctaa aaagtgaaaa actctataaa
     8281 tacttaaata tagagtaaag ttttaaaggc gagttcagct cgccttgtta ttatttttaa
     8341 agttttgctg taatccattc ttgagcaagt gacaatgctt gcaataaatg ttgtggttct
     8401 gagcctccgg ccattgccat atcaggacgt ccgccccctt tacctcccac tttttcagcc
     8461 attgcaccaa ctaattgacc agcattgact ttatcggtta aatctttggt cacacctaca
     8521 atcaaattga ctttatcttc cgccgtagtc gcaaatacaa caactgcact gcctaattga
     8581 ttttttaggt catcaaccat cgttctcaat gcttttggtt caacattctc taattgttga
     8641 atcactacat tcacgccatt gatttgggta gcttgtttgg ctaaatctga acctgcttgt
     8701 gctgcaagtt tatcttttag ctgttgaagc tctttttctg tacgtttagc cttctcttgt
     8761 aattgaacaa ttttctcaac gaatgaatga ctgtctgctt ttaacaattc agcactttgt
     8821 tgaacggctt gttgtaaggt atgcaaccaa gcaatcgcat tttcaccagt gacagcctct
     8881 atacggcgta cacctgctgc taccgcacct tcagaggtaa ttttgaataa gccaatatca
     8941 ccggtttgtt taacgtgtgt gccgccacaa agttcaatcg agaaatctga catttccact
     9001 acacgcacta cttcgccata tttttcacca aataacgcca ttgcaccttt cgcttttgca
     9061 gattcaatat ccatttcttc aatgattacc tgaatatttt cacgcacttt agcattcacg
     9121 atacgttcaa cttcttcaag ttgtgattta gtaattgctt ccggctgaga aatatcaaaa
     9181 cgtaaaatat tttctgacac taatgagcct ttttgagcaa cgtggtcgcc tagcacttgg
     9241 cgtaacgcag aatgcagtaa gtgggtagca ctgtggttta atgtaatagc atgacgacgc
     9301 tctctatcta cgatcgtatt aactttgtcg ccaacagaaa gtgagccgga agttaattgc
     9361 ccaatatgcc cgaatacttg accatatttt tgcgtatcta ctacctcaaa attgcaaatt
     9421 tctgcggaaa tttgaccgct atctcccact tgtccgccca tttctgcata gaatggggtt
     9481 ttatctaaaa tcactaccgc attttcaccc gattgaatag actcaaccga ttttccatta
     9541 ctaaataacc caattacagt agcatcattt aagctggttt cagtatatcc actaaagcat
     9601 gttttactgt ccactttaat cacattgtta taatctgtgc caaagctact gctggctttc
     9661 gcacgctcac gttgtgcggt catttctgca ttgaagccgt cttcgtcaat tttaatatca
     9721 cgttcacggc aaacatctgc ggttaaatct aacgggaagc cataagtatc ataaagtttg
     9781 aacgcaactt cgccagataa ggtgttattt tcaaccttcg ctaatgcttc ttctaataaa
     9841 ttcaatccac gctcaagagt acgggcaaat tgctcttctt ctgctttgag tgtcttttga
     9901 atatgcgctt gtttttgggt cagcacctca ccagcttgcc ccattaccgt tgcaagtgtt
     9961 ggcaccaatt tatagaaaaa ggcttctttt gcacccaaaa tattaccgtg acgaaccgcc
    10021 cggcggataa tacggcgtaa tacatagcct cttccttcat ttgaaggtaa aacaccatcg
    10081 gcaatcagat aggaacaagc acgaatatgg tctgccacta cacgtaaaga tttattatct
    10141 aaatctttca cattaagcaa agtagcgact tctttaatta aagtttggaa aatatccgtt
    10201 tcgtagttgg agtttacgtg ttgcattacc gcagtcatac gttctaaccc catccctgta
    10261 tctacagaag gcttaggcag tttttccatc gtaccgtctg ctaaacgatt gaactgcata
    10321 aatacaatat tccatacctc aatatagcgg tcgccatctt cttctgggct acccggtaag
    10381 ccaccccaat gttcaggccc gtgatcatag aaaatctcgg tacaagggcc acaagggccg
    10441 gtatcgccca tcgcccagaa gttatctgaa gcataaggtg cgcctttatt atcgccaatg
    10501 cgaataatat gttccgatgg tacacctact tctttatgcc aaatatcgta ggcttcatca
    10561 tcagtttcat acacagttac atataatttt tcttttggta agccaagcca ttgtggagaa
    10621 gttaaaaact cccaaccgaa tttgattgca tcttgcttga agtaatcacc aaagctgaaa
    10681 ttccccatca tttcaaaaaa agtatgatgg cgagcggtat aacctacgtt ttctaaatca
    10741 ttgtgtttac cacctgcacg tacacaacgt tgtgccgtag tcgctctgct ataagggcgt
    10801 ttttctaacc caagaaaaac atctttgaat tggttcatgc ccgcattggt aaataataat
    10861 gtcggatcat tctcgggaac aagagagctg cttggaacaa tcgtatgtcc tttagtttgg
    10921 aaaaactcta gaaaagattg tctgatatct gaagttgttt tcattaaaaa tcactctttt
    10981 tgtaaataga agttaaacat ttagttatct tgctatttta cacatttttt atgctattgg
    11041 gggagctata tgctacttat tttttcaatt aaataatttt tgtaaatttt cccaaaaaaa
    11101 taaccgcttg taagacagat aaactgtctc aagcggttat ttttcattca ctttcttgct
    11161 attcatctgc taatggcaca accagcatat caacagtgat attattcatg actcgacgag
    11221 ttgaagacat aaatttactc cagaagtcct gatgatggcc agtgatgagt aaatcgacat
    11281 tgtgtttttc tacggcctct tccaaaactt gactaaaatc tcccgttccg tttaaacatt
    11341 cagatacagg ataatttact tttgaggcaa gttcagccag tgccgtattt gtttcttcaa
    11401 caacactgta ttgtaccgaa gacatattaa tatcaattaa tcctgtatag agatccgaaa
    11461 aattaacatc aacgtgaatg agggaaagtt tagctccgca tttttctgct aacgcagccc
    11521 ccttacgcac taaaacaaga ctttcttcag aaagatccac tgcaactaag atatgtttat
    11581 acataatatg ctccttgctt gtgagagttg tttactttg
//
LOCUS       DANLUQ010000074        11084 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.074, whole genome shotgun sequence.
ACCESSION   DANLUQ010000074 DANLUQ010000000
VERSION     DANLUQ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11084
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.074"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            109..2001
                     /gene="mnmG"
                     /locus_tag="PXB27_001499"
     CDS             109..2001
                     /gene="mnmG"
                     /locus_tag="PXB27_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDL5346276.1"
                     /translation="MIYNQIYDVIVVGGGHAGTEAALAPARMGLKTLLLTHNVDTLGQ
                     MSCNPAIGGIGKGHLVKEIDAMGGLMAKATDVAGIQFRTLNSSKGPAVRATRAQADRV
                     LYRNAVRTALENQPNLDIFQQEVVDILVENNRAVGAVTKMGLTFKARAVVLTAGTFLA
                     GKIHIGLDNYAGGRAGDPAATMLADRLRDLNLRVDRLKTGTPPRLDARTINFDVLAKQ
                     HGDEVLPVFSFMGDVSQHPRQIPCYITHTNDQTHDLIRNSLDRSPMYTGIIEGVGPRY
                     CPSIEDKVMRFADRNSHQIYLEPEGLNTIEVYPNGISTSLPFDVQMGIVNSMKGLENT
                     RIIKPGYAIEYDYFDPRDLKPTLETKAIDGLFFAGQINGTTGYEEAGAQGLLAGINAA
                     LQVQGKEAWYPTRDQAYTGVLVDDLCTLGTKEPYRVFTSRAEYRLLLREDNADIRLTP
                     KAHELGLIDEARWVRFNQKMEAIEQERARLKSTWAHLQMPNLDELNALLANPLAREAS
                     GEDLIRRPEMSYEKLTQIALFAPAIEDKQAAEQVEIAIKYQGYIEHQYNEIERHKRHE
                     NTLIPVEFDYDKVESLSNEVRAKLMQHRPVSIGQASRISGITPAAISILLVNLKKQGM
                     LKRGEL"
     gene            2096..2320
                     /locus_tag="PXB27_001500"
     CDS             2096..2320
                     /locus_tag="PXB27_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346277.1"
                     /translation="MIYLRKLGFSPFDHQMFAQPSWYFCNALVHTNYENLRQGVFVTK
                     AYLVMFLENLLFEKSHSLSNRALRINENND"
     gene            2333..2947
                     /gene="rsmG"
                     /locus_tag="PXB27_001501"
     CDS             2333..2947
                     /gene="rsmG"
                     /locus_tag="PXB27_001501"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDL5346278.1"
                     /translation="MLAKLNRLLAQAEINLTDQQKEQLVGFVRLLDKWNKAYNLTSVR
                     NPDEMLVKHILDSLVVSRHLQGETFIDVGTGPGLPGIPLAIANPDKQFVLLDSLGKRI
                     TFIKNALRELGIKNVTPVQSRVEEYTESQFDGVLSRAFASLNDMIDWCYHLPTENGKF
                     YALKGVYDEQEIIEIKQPIQLVEVIKLEVPELVGERHLVVLKKP"
     gene            3215..4137
                     /locus_tag="PXB27_001502"
                     /pseudo
     CDS             3215..4137
                     /locus_tag="PXB27_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248294.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            4160..5206
                     /locus_tag="PXB27_001503"
     CDS             4160..5206
                     /locus_tag="PXB27_001503"
                     /inference="COORDINATES: protein motif:HMM:NF016497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDL5346279.1"
                     /translation="MQIQNPVLPGFNPDPSIVRVGDTYYIATSTFEWFPGVRIHESKD
                     LVHWNLVKNVLDTTTLLDMRGNPSSGGIWAPDLSYAEGKFWLVYTDVKITDGAFKDMT
                     NYLTTAKEIQGPWSAPIKLNGIGFDASLFHDDDGRKYLVQQTWDHREYHHPFDGITLT
                     EFDVATMKLKPETQRTIFEGTEVKLVEGPHIYKINGEYYLFAAEGGTVFTHQEVVARS
                     KTLEAHSFQLAPNTPFITNFDTPNSYLQKQGHGSLVDTPSGEWYYASLTARPWNHATE
                     SATDPRGWSTLGREAAIQKVEFDQDGWPYIVGGHGGKTFVEAPKDAIFTEAPIDHSQH
                     DEFDSSTLDINWNT"
     gene            5227..5847
                     /locus_tag="PXB27_001504"
     CDS             5227..5847
                     /locus_tag="PXB27_001504"
                     /inference="COORDINATES: protein motif:HMM:NF036826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,4-xylosidase"
                     /protein_id="HDL5346280.1"
                     /translation="MGEVGNGVLKLIGRGSLANTHDLSLIARRWQAFYFDAETKVKFT
                     PFSYQSMAGLTNYYNHSHWSWIFITKNDQGQQVIEVAENKGGLRNGQYTSYLKDKAIV
                     IPDGTEYVWFKTKVRKETYRYEYSFDGKQWHTIPVELDAAVLSDDYVLRSYGGFFTGA
                     FVGLAAVDYSSYESTAEFSYFDYKELGDKQLTDGSFSWEKSESCFG"
     gene            5866..6846
                     /locus_tag="PXB27_001505"
     CDS             5866..6846
                     /locus_tag="PXB27_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL5346281.1"
                     /translation="MKQRRFGKTNKMVSEIGLGTWQLGTKWGEPFNHQEAMAILETAY
                     EQGINFIDTADVYNNGQSEKAIGDILKKYPDFFYVVTKCGRALNPHTAEMYTPQAIEK
                     FVDGSLSHLGTEKLDMILLHCPPTLVYRNDEIFTKLEQLKAGGKLIDYGVSIETAEEG
                     LLAMEYDIAAMEVIFNMFRLKPLEQLFPTAQQKNVGIIARVLLASGLLTGRYNACTQF
                     GKDDHRTFNRNGEAFDKGETFSGVDYQLGLQAVEELKALFGTEDLIPYALRWVLMNEA
                     VSTVIPGASKVSQVITNAAVDNFPPLTENEMQKVEDIYNRLIRPSVHHLW"
     gene            complement(6908..8248)
                     /locus_tag="PXB27_001506"
     CDS             complement(6908..8248)
                     /locus_tag="PXB27_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5346282.1"
                     /translation="MFSFLKASPPAEQKVEASRVDAEYRKLRWQVFAGVFIGYAAYYL
                     IRKNFSLAMPYLIDEYGFTKADLGTVGVALSLAYGFSKFIMGNVSDRSNPKYFITIGL
                     LGSAIVSLVFGLVPGVLSSIPIMIILAALNGWFQGMGYPPGAKTMTNWFSTSERGVWW
                     SWWNVSHNLGGGLIGPLAILGLSIFGVWQSLFYLPALIAIVLAFVMFYLMRDTPESQG
                     LPPIDEYKGEKVVEKVESAHTLSSKDIFYKYILNNKFLWAIAIANVFVYFIRYGIIDW
                     APTYLKEVKHFSVDKQSWAYFLYEYAGIFGMLASGYLSDKVFKGHRAPPMLLFLAGVL
                     IAIVVYWKNPAGNPLIDNICLVAIGFLIYGPVMMIGLQAADLVPRVATGTATGLTGLF
                     GYLLGSASAGYVMGKLVDMFGWDGGFYALILSCFLAFAFIALTLFKKTSKQLEV"
     gene            complement(8438..9511)
                     /gene="glpQ"
                     /locus_tag="PXB27_001507"
     CDS             complement(8438..9511)
                     /gene="glpQ"
                     /locus_tag="PXB27_001507"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDL5346283.1"
                     /translation="MKFKFKTLVAALALSATALGAYAQQSDKLVIAHRGASGYLPEHT
                     LESKALAFGQQADYLEQDLAMTKDNRLIVIHDHFLDGLTDVAKKFPERKRADGRYYVV
                     DFTLDEIKTLEMTENFEVKDGKQAQVYPNRFPMWQSHFTIHTLEEELEFIQGLEKSTG
                     KKIGIYPEIKAPWLHHKEGKDIALETLKVLKKYGYDSKTSPIYLQTFDFNELKRIKTE
                     LLPKLGMDVKLVQLVAYTDWHETEEKDKNGNWVNYDYDWMFKDGAMAEVAKYADGVGP
                     GWYMLVDKEKSKVGNIVYTPLVKELAKYKMELHPYTVRKDALPDFFQNVDEMYDALLN
                     KAGATGVFTDFPDTGVEFLKKQK"
     gene            complement(9581..11026)
                     /gene="glpT"
                     /locus_tag="PXB27_001508"
     CDS             complement(9581..11026)
                     /gene="glpT"
                     /locus_tag="PXB27_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HDL5346284.1"
                     /translation="MFGPFKPAPHIAELPADKVDARYKFLRWQVFAGIFFGYAAYYFV
                     RANFDLAQKGLIEAGMYTKTDLGVIGTAAGLAYGLSKFVMAGMSDRSNPKVFLPTGLL
                     LSGLCMTLMGLMPWATSSILVMWVMIFLNGWFQGMGWPPCGRTMVHWWSKSERGTIVS
                     IWNTAHNLGGMVPGAMVLLASALYFSEYGVEATAKDVWQQALYFPGIAAMIAAIPIYL
                     VMKDTPQSCGLPPVEKWRNDYPDDYNEKTYEHDLSTKEIFVTYVLKNKLLWYIAIANV
                     FVYLIRYGVLKWSPVYLGEVKHFNIKGTAWAYTIYELAAIPGTLLCGWVSDKLFKGKR
                     GLTGFIFMILTTAAVVAFWLNPATPESEIAQYAGHAWYENPYQLMDFIWMTTIGFLIY
                     GPVMLIGLHALELAPKKAAGTAAGFTGLFGYLGGTVAASAVVGWAADKFGWDGGFYVM
                     IGGGVLAILLTFIVMVEEGKHKAKLEDHYGK"
BASE COUNT         3204 a         2342 c         2393 g         3145 t
ORIGIN      
        1 tgattttgat cctagcgttg ctaaaaatct atttccctga tgatcccttt ttacgctaaa
       61 atatgcgttt actttgtagc agaatttatt tgaataaaaa ggcgatatat gatttataac
      121 caaatttatg acgtgatcgt tgttggtggc ggtcacgccg gaactgaagc agcacttgct
      181 cccgctcgaa tgggcttaaa aaccctgctt ttaacccata atgtagatac cttaggacaa
      241 atgtcttgta acccagccat cggtgggatc ggtaaaggtc atctggttaa agagatcgat
      301 gcaatgggcg gtttaatggc aaaagccact gatgttgccg ggatccaatt ccgtactcta
      361 aacagctcga aagggcctgc agtgcgtgct actcgagcac aggcagaccg tgtgctttac
      421 cgcaatgcag tgcgtacagc attagaaaat caaccgaatt tagatatttt ccagcaagaa
      481 gtggtcgata ttttggtgga aaacaaccgt gcagtgggtg cagttaccaa aatgggctta
      541 acgtttaaag cccgtgcggt agttttaacc gctggtacgt tcttagcggg taagatccac
      601 attgggttgg ataactatgc aggtggtcgt gccggcgatc ctgctgcaac aatgttggca
      661 gatcgtctgc gtgatctgaa tttgcgtgtt gatcgcctta aaacaggtac accacctcgc
      721 ctcgatgccc gtaccatcaa ctttgacgta cttgccaaac agcacggcga tgaggtgtta
      781 cctgtcttct cttttatggg cgatgtctca cagcacccac gccaaattcc gtgctacatc
      841 acccacacta acgatcaaac ccacgattta attcgcaaca gtcttgatcg cagcccgatg
      901 tacaccggta ttatcgaagg ggttggtcca cgttactgcc cgtctattga agataaagta
      961 atgcgttttg ccgatcgtaa tagccaccag atctaccttg aaccggaagg cttaaatacg
     1021 attgaggttt acccgaacgg gatctccacc agcttgccgt ttgatgtgca aatggggatc
     1081 gtcaactcaa tgaaaggctt ggaaaacacc cgcatcatca aaccgggcta tgcgattgag
     1141 tacgactact tcgacccgcg tgatttaaaa ccaacccttg aaaccaaagc gattgacggt
     1201 ttattctttg ccggacaaat taacggcacg accggttacg aagaggcagg tgcgcaaggt
     1261 ttgctggcag ggattaacgc agcgttacag gttcaaggta aagaggcgtg gtatccaacc
     1321 cgtgatcaag cctacaccgg cgtgttggtg gacgacctct gtacattagg caccaaagag
     1381 ccataccgtg tgtttacctc tcgtgccgaa taccgtctgt tgctgcgtga agacaatgcg
     1441 gacattcgtt taacaccaaa agcccacgaa ctaggattaa ttgacgaggc acgttgggtg
     1501 cgtttcaacc aaaaaatgga agcgattgag caagaaagag ctcgtttgaa atccacttgg
     1561 gcacatttgc aaatgccaaa tctggacgaa ttgaacgcgt tattggctaa tcctttagcc
     1621 cgtgaagcaa gcggtgaaga cttaatccgc cgtcctgaaa tgagttacga aaagctgacc
     1681 caaatcgccc tatttgcccc tgctattgaa gacaagcaag cggcagaaca ggtggaaatt
     1741 gcgatcaaat atcagggcta tattgagcat caatacaacg aaatcgaacg ccataaacgc
     1801 cacgaaaaca ccctgattcc ggtagagttt gactacgaca aagtcgaaag cctctccaac
     1861 gaagtgcgag ctaaattgat gcaacaccgc cctgtctcca tcgggcaggc aagccgcatt
     1921 tccggcatca caccggcagc gatttccatt ctgttggtga atttgaagaa gcaaggaatg
     1981 ttgaaacggg gtgagctgta aatactacaa gcggttggtt ttgcagaaat ttttgcaaaa
     2041 ttgaccgctt gtattctttg gagaaggaaa tacccgaact accgccgtat ttttaatgat
     2101 atatctgcgt aaactcggtt tttcgccgtt cgatcaccaa atgtttgccc aaccttcgtg
     2161 gtatttctgc aatgcacttg ttcataccaa ttatgaaaat ctgcgacaag gtgtatttgt
     2221 gactaaagcg tatttagtga tgtttcttga aaatttattg tttgaaaaaa gtcattcgtt
     2281 aagtaatcgg gcattgcgta ttaatgaaaa taatgattaa ggaatttgca aaatgttagc
     2341 taaattaaac cgcttgcttg ctcaagccga aataaatttg accgatcaac aaaaagagca
     2401 gttagttggt tttgtgagat tattggataa atggaataag gcgtataacc tcacttccgt
     2461 tcgcaaccct gatgaaatgt tggttaaaca tattttggac agccttgttg ttagtcgtca
     2521 tttacaaggc gaaacgttta ttgatgtcgg cacggggccg gggctaccgg gcattccgct
     2581 ggcgattgct aaccctgata aacagttcgt gctactagac agcttaggca aacgcatcac
     2641 ttttatcaaa aatgcgttgc gtgagcttgg tattaaaaac gttaccccag tgcaaagtcg
     2701 agtggaggaa tacactgaaa gccagtttga cggcgtatta agccgagcct ttgcctcgct
     2761 taatgatatg atcgactggt gttatcattt accgactgaa aacggtaaat tttatgcatt
     2821 aaaaggcgtg tatgatgagc aggaaataat tgaaatcaaa caaccgattc agctagtgga
     2881 agtgattaag cttgaagtac ctgagttggt aggagaacgg catttagtag tgttgaaaaa
     2941 gccctaatac attggaagtt taatgataca agcggtcgaa tttgtaaatt tttttgcaaa
     3001 tctgcctgct tgtgttaact atttttcata aagcatacct ggttaataag gtgtgctttt
     3061 tttataacct attatttcat ctgatttatt tctattttgt gatgtgcttc acaaaaacaa
     3121 aaaaacataa tgtagtaaaa aatattcgta attgaagccc ttatttcttt acctgaaaat
     3181 aagcgaactt aattatataa cgaggaggtt cgtaatgaac agggtaacaa ataaggcaga
     3241 tgagtttgct aaactcagta cgtttgaacg tattgcttat ggtagtggcg acttagccca
     3301 aaatttagtt tttggtacag ttggtggttt cttactcact tacttaacta ctgtaaacgg
     3361 catttctgcc gtagcaggag caacgatttt cttaattgta aaatggggaa atgtgttttg
     3421 ggatccgttt gtcggtgcct ttattgacaa aagcaagccc tcagcacagg gtaaataccg
     3481 cccgtattta ctgaaatttg ggattcctct tgtggtttta gcttcactac tatttcttcc
     3541 tcttacctaa aatataatat tgggcattct gagtggattg cctccgtcgg tttattaggc
     3601 tctatccctg ggattttcct tgtattctta tggccgaaac tacgtcgcac tatcggtaaa
     3661 aagaactttt tctatctatt tttagggatt ttcgttctag ggcaactttg tctatgggta
     3721 tggcaatttg actccttcaa atactctcca accttaggct atatcggtcg tttcttacaa
     3781 caatggggat taacttctgc taccggtttt atgtgggcgt tagtgcctga ggttgttacc
     3841 tttggagagt ttaagtcgaa aaaacgtgta gcaggtatta ttaatgcgtt aatgggcttg
     3901 ttctttaaaa tcggcttggc gttaggcggt attattccgg gttatattaa tgcggtttat
     3961 ggttttaacg gctcgctttt ggctcaaacc gaagaggctt tatttggtat caatctttca
     4021 atgatttggg ctccaattat tttagcgatt attgcaggat ttgtgatgag tcgctatccg
     4081 ttaagcgata ctgatattga tcatatcaac gaaaaaatta ctgcttattc caaataattt
     4141 gagtatagga gaatagatta tgcaaattca aaatccagtt ttacccggtt tcaatccaga
     4201 tccatctatt gtgcgtgtgg gtgatactta ctacattgca acttctactt ttgaatggtt
     4261 tccgggagtg agaattcacg aatcgaaaga tttagtgcat tggaatttag ttaaaaatgt
     4321 cttagataca accacgttgt tagatatgcg aggcaatccc tcttcaggtg gtatctgggc
     4381 tcctgatctt tcttatgcgg aaggtaaatt ttggttggtt tataccgatg taaaaattac
     4441 agacggtgca ttcaaagata tgactaacta tctaactact gccaaagaaa ttcaaggtcc
     4501 ttggtcagct ccgattaaat tgaatggtat tgggtttgat gcttcgttgt ttcacgatga
     4561 tgatgggcga aaatatttag tgcaacagac ttgggatcac cgtgaatatc atcacccctt
     4621 tgacggtatt acgttaacgg aatttgatgt tgccacaatg aaactcaaac ctgaaaccca
     4681 gcgtacgatt tttgaaggta cagaagtgaa attggtggaa ggtccacata tttataaaat
     4741 taatggcgaa tattatctgt ttgcggcgga aggcggcaca gtatttacgc atcaagaagt
     4801 agtggctcgt tctaaaacat tggaggctca ctctttccaa ctggctccaa atacaccgtt
     4861 tattactaat tttgatacac ctaattctta cctacaaaaa caagggcacg gttcattagt
     4921 tgatacccca agtggtgaat ggtattacgc ttccctcaca gcaagacctt ggaatcatgc
     4981 aacagaatcc gcaaccgatc ctcgtggttg gtcaacatta gggcgtgaag ccgccattca
     5041 aaaagtagaa tttgatcaag acggctggcc ttatattgtg ggtggacacg gtggaaaaac
     5101 ctttgtagaa gccccaaaag atgcaatttt taccgaagct cctattgatc attctcaaca
     5161 cgatgagttt gatagctcaa cgttagatat taactggaac acttaagagt gccttttagt
     5221 aaagagatgg gcgaagttgg taatggtgtt ttaaaattga ttgggcgagg ctctcttgca
     5281 aatacacacg atttatcgct aatcgcacgc cgttggcaag ccttctattt tgatgcggaa
     5341 accaaagtga aatttacacc gttttcttat caatctatgg caggtttaac taactattac
     5401 aatcactcac actggtcttg gattttcatc accaaaaatg atcaaggaca acaggtgatt
     5461 gaagttgcag aaaataaagg tggactcaga aatgggcaat atacttctta ccttaaagat
     5521 aaagcaattg tcattcctga tggaacggag tatgtttggt ttaagactaa agttcggaaa
     5581 gaaacttacc gttatgaata ttcattcgat ggcaaacaat ggcacactat tccggttgaa
     5641 ttagatgcgg cagtgctttc agatgattat gtgttacgca gttatggcgg tttcttcaca
     5701 ggagcttttg tcgggctggc tgcagtggat tactccagtt atgaatctac agcagaattt
     5761 agctactttg actataaaga gttaggcgat aagcaactta cagacggctc attctcttgg
     5821 gaaaaatctg aaagttgctt tggctaaaat gaggagaata tcacaatgaa acaacgcaga
     5881 tttggtaaaa ccaacaaaat ggtgtcagaa attgggttag gcacttggca gcttgggacg
     5941 aaatggggtg aaccgttcaa tcatcaagag gcgatggcaa ttttagaaac cgcttatgag
     6001 caaggcatca attttattga taccgctgat gtgtataaca acgggcaaag cgagaaagcc
     6061 attggtgata ttttgaaaaa ataccctgac tttttctatg tcgttaccaa atgtggacgt
     6121 gcgttaaacc cgcatactgc tgaaatgtac acgccacagg caatcgaaaa atttgttgat
     6181 ggtagtttaa gccatcttgg cacggaaaag ttggatatga ttttgctcca ctgcccgcca
     6241 acgttggttt atcgcaatga tgagattttt acgaaattgg aacagcttaa agccggcgga
     6301 aaattaatag actacggtgt gagtatcgaa acggcagagg aaggcttgtt ggcaatggag
     6361 tatgacattg cggcaatgga agttatcttc aatatgttcc gcttaaaacc attggagcaa
     6421 ctcttcccaa ccgctcagca aaaaaatgtg ggtattatcg cccgtgtact gttggcaagt
     6481 ggtttgctta caggcagata taatgcctgc acccaatttg gcaaagacga ccaccgcaca
     6541 tttaaccgca atggtgaagc cttcgataaa ggcgaaacct tttcgggtgt ggattatcaa
     6601 ctcggtttac aagcggtgga agaattgaaa gccctattcg gcacggaaga tttaattcct
     6661 tacgccctac gttgggtatt gatgaatgaa gctgtgagta cagtaatccc gggagcaagt
     6721 aaggtttcac aagtaattac taacgcagcg gtggataatt tccctccact cactgaaaat
     6781 gaaatgcaaa aagtggaaga tatttacaac cgcttaattc gcccaagtgt gcatcatttg
     6841 tggtaattta tccaacgcta taaaaaatgg aactctaact tgagttccat ttttgtcaat
     6901 tttagcctta aacctctaac tgtttagagg ttttcttaaa cagagtcaat gcaataaacg
     6961 caaaggctaa gaaacaggaa aggatcaggg catagaaacc accgtcccag ccgaacatat
     7021 caaccagttt acccatcaca taacctgcac ttgcagagcc taacaagtag ccgaataaac
     7081 cggttaaacc tgttgctgta ccggtggcaa cacgaggcac taaatccgct gcttgtaaac
     7141 cgatcatcat caccggcccg tagattaaga aaccaatcgc cactaagcag atgttgtcga
     7201 ttaacgggtt gccggctggg tttttccagt acaccacaat cgcaattaac acaccagcaa
     7261 ggaataacaa catcggcggc gcacggtgac ctttgaatac tttgtcgctt aaataaccgc
     7321 ttgcaagcat accgaagata ccggcatatt catacaagaa gtacgcccag ctctgtttat
     7381 ccacagagaa atgtttcact tcttttaagt aagtcggtgc ccagtcgata atgccgtagc
     7441 gaatgaaata gacaaacacg ttggcaatcg caattgccca caggaatttg ttgtttaaaa
     7501 tatatttgta gaaaatgtct ttggaagaga gtgtgtgggc agactccact ttttccacca
     7561 ctttctcgcc tttgtactca tcaatcggcg gtaagccttg agattccggc gtatcacgca
     7621 tcaaatagaa catcacaaac gccagtacaa tcgcaatcaa cgccggcaga tagaacaggg
     7681 attgccacac gccaaaaata gataagccta aaatcgccaa cggcccgatt aaaccgccgc
     7741 ccaagttgtg cgacacgttc caccagctcc accacacgcc acgttcagag gttgagaacc
     7801 agttggtcat cgttttcgcc cccggcggat agcccattcc ttggaaccag ccgtttaacg
     7861 ccgccaaaat gatcataatt ggaatagacg aaagcacgcc cggtactaaa ccgaacacca
     7921 gactcacaat cgctgaaccg agcaagccga tagtgataaa gtatttcggg ttagaacggt
     7981 cggaaacgtt acccataata aatttactga agccgtaagc gagtgataat gccacgccca
     8041 ccgtacctaa gtctgctttg gtgaagccat attcatcaat caaatacggc attgccaacg
     8101 agaagttttt acgaattaaa taatatgccg catagccgat aaagacgcct gcaaacactt
     8161 gccaacgtaa tttgcgatat tccgcatcaa ctctgctggc ttccactttt tgttctgctg
     8221 gtggagaggc ttttagaaag gaaaacattt tactactcct ttaagaatga aatcctatgg
     8281 ctactataat ctttcatttg gagcagaaaa agtggatttt tgaaaatttg tgagcaagat
     8341 taaaattatt ttgagcgaaa acgaaaagcg gttgaatttc cttctttttt tgcaaaaaat
     8401 ttgagaaatc taaccgcttg tttcacttaa aaatcagcta tttctgcttt ttcaaaaatt
     8461 ccacgcccgt atcagggaag tcagtaaaga cgcctgttgc acccgcttta ttcagcaggg
     8521 catcgtacat ttcatcgaca ttttggaaaa agtccggcag agcatctttg cgcacggtgt
     8581 atgggtgaag ttccattttg tacttcgcca actctttcac taatggcgtg taaacaatgt
     8641 tgccgacttt agatttttct ttatccacca acatatacca gcccgggccg acaccatcgg
     8701 catattttgc cacttctgcc atcgcaccgt ctttgaacat ccagtcgtag tcgtaattaa
     8761 cccagttgcc gtttttatct ttctcttcgg tttcgtgcca atcggtgtag gcaactaact
     8821 gcaccaattt tacgtccata cccagtttcg gtagtaattc ggttttgatc cgtttcagct
     8881 cgttgaaatc gaaggtttgc aggtaaattg gtgaggtttt gctgtcgtag ccatattttt
     8941 tcaacacttt cagggtttcg agtgcaatgt ctttaccctc tttatggtgc aaccaagggg
     9001 ctttaatttc aggatagatc ccaatttttt tgccggttga tttctctaat ccttgaataa
     9061 attccagctc ttcttccaaa gtgtgaatgg tgaaatggga ctgccacatc gggaagcggt
     9121 tcgggtaaac ttgtgcctgc ttgccgtctt tcacctcaaa attctcggtc atttcaaggg
     9181 ttttaatctc atcaagggtg aaatccacca cataataacg accgtctgct cgtttacgct
     9241 cagggaattt tttcgccaca tcagttaaac cgtctaaaaa gtggtcgtga atcacaatca
     9301 ggcggttatc tttggtcata gcaagatctt gctctaaata gtccgcttgt tgcccgaatg
     9361 ctagagcttt agattcaagc gtatgctcag gtaaataacc gcttgcacca cggtgggcaa
     9421 tcactaattt atcagattgt tgtgcatagg cacccaatgc tgtcgcggac aatgctaatg
     9481 cagcaaccaa tgttttgaat ttaaatttca tagaattacc tcatataatg taaaaataaa
     9541 aggaagaaca aaaatgtcct tccttttgat aagttattta ttatttacca tagtgatctt
     9601 ctaattttgc tttgtgcttg ccttcttcaa ccatcacaat gaaggttaag agaatggcta
     9661 atacaccacc accgatcatc acgtagaaac cgccgtccca gccaaattta tctgctgccc
     9721 agccaactac tgcagaggct gccactgtac cacctaagta accgaataaa ccggtgaaac
     9781 ctgctgctgt acctgctgct tttttcggtg caagttcaag ggcgtgtaag ccaatcaaca
     9841 ttaccgggcc gtagattaag aaaccgatag tggtcatcca aatgaagtcc attaattgat
     9901 atgggttttc ataccaagca tggcctgcat attgtgcaat ttcagattcc ggtgtcgcag
     9961 ggttgagcca gaacgcaacc actgctgcgg tggtaagaat cataaagatg aaaccggtta
    10021 aaccacgttt gcctttgaac agtttgtcag agacccaacc acagagtaat gtacctggaa
    10081 tcgcagctaa ttcataaatg gtatatgccc atgctgtacc tttaatgttg aagtgtttta
    10141 cttcgcctaa gtacaccggc gaccatttca atacgccata gcggattaag taaacgaaga
    10201 cattcgcaat ggcaatgtac cataacagtt tgtttttcag cacataagtc acgaaaatct
    10261 ctttggtgct taaatcgtgt tcataggttt tttcgttata gtcgtcaggg tagtcgttac
    10321 gccatttttc aactggcggt aaaccgcaag attgtggagt atctttcatc acaaggtaga
    10381 ttggaatagc tgcgatcatt gctgcaattc ccgggaaata aagggcttgt tgccatacat
    10441 cttttgccgt tgcttctaca ccatactcac tgaaatagag cgcacttgct aatagcacca
    10501 tcgcccccgg caccataccg cctaagttgt gagcggtatt ccagatagaa acgatagtac
    10561 cacgctcaga tttagaccac cagtgaacca ttgttcgtcc acacggtggc caacccatac
    10621 cttgaaacca gccgtttaag aagatcatta cccacattac taaaatgctt gatgttgccc
    10681 aaggcattaa gcccataagg gtcatacata gacctgaaag taacaatccg gtcggtaaaa
    10741 atactttcgg gttagagcgg tcggacattc ctgccatcac gaattttgat aaaccgtagg
    10801 ccaggccggc agctgtacca atcacaccga gatcggtttt tgtatacata ccagcttcaa
    10861 tcaagccttt ttgtgctaag tcgaagtttg cacgcacaaa atagtaagcg gcatagccga
    10921 agaaaattcc tgcaaacact tgccaacgca agaatttata tcttgcatcg actttgtccg
    10981 ccggtaattc cgcaatgtgc ggagcgggtt taaatggacc aaacatagta ttctccaata
    11041 ttacttagta aaacctctga gtaattcctt tttaggaaat tagg
//
LOCUS       DANLUQ010000075        11009 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.075, whole genome shotgun sequence.
ACCESSION   DANLUQ010000075 DANLUQ010000000
VERSION     DANLUQ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 11009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11009
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.075"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(165..1403)
                     /gene="pepT"
                     /locus_tag="PXB27_001509"
     CDS             complement(165..1403)
                     /gene="pepT"
                     /locus_tag="PXB27_001509"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDL5346285.1"
                     /translation="MKQSLLNRLLRYTQIHTTSDPKSQTVPSSQQQWDLLHLLKTEVE
                     AMGLSEIYLDPNGYLFASLPANIDKTLPVLGLIAHVDTSPDFNGENVKPQVIEAYQGG
                     NILLNGSGDYLSPTDFPNLNKLIGDTLVTTDGTSLLGADDKSGIAIILSAIEYLQANP
                     HIPHGKIRIGFTTDEEIGRGADHFDVNAFGADVAFTFDGGPLGELQYENFNADAATVT
                     FFGRSVHPGTAKGKMINALERACEFQHRLPPHLTPATSEGREGFIMLHGIEGGIDKAE
                     IHYILRSFERDELAAFGKMLNDTLAEMQQIYGEHCGKVEIVEQYRNMREVLDDYGYLV
                     EIAENAMREQGIEPNLEPIRGGTDGSRLSFMGLPCPNLFTGGENFHGRFEFISVDTME
                     KATKVVIDICRKFADYDFNK"
     gene            complement(1423..1812)
                     /locus_tag="PXB27_001510"
     CDS             complement(1423..1812)
                     /locus_tag="PXB27_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HDL5346286.1"
                     /translation="MYMLDTNTVSYFFRGNANVVAKLKSINPERLCISSVTAAELVYG
                     VAKRNNGQLSQFLDYFLSSVKVLDWNYRCAELYGKLRAEMEKNGKVMGVQDQMIASHA
                     LAEECILVSSDQAFKMVPDLKLENWLH"
     gene            complement(1813..2073)
                     /locus_tag="PXB27_001511"
     CDS             complement(1813..2073)
                     /locus_tag="PXB27_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HDL5346287.1"
                     /translation="MEKIARLFRNGRNQAVRLPVEFEFDAEQVYVSKKENGDILLSLR
                     SRNTEHWQRLFELLPAVVCDETFLDTKERNQSFEQRDPFEVA"
     gene            complement(2189..2401)
                     /locus_tag="PXB27_001512"
     CDS             complement(2189..2401)
                     /locus_tag="PXB27_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HDL5346288.1"
                     /translation="MRDEYDFSNAVKNPYAKGLKSKSVVTIRIEDDVISYFKQLAEEV
                     NLPYQTLINLYLKDCVEQKRKPTISW"
     gene            complement(2402..2689)
                     /locus_tag="PXB27_001513"
     CDS             complement(2402..2689)
                     /locus_tag="PXB27_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HDL5346289.1"
                     /translation="MEEINGFEWDSEKALRNQQKHGVSFIEAVSVFYDNNAMLIPDPD
                     HSSREERFLLLGRSNKDNLLVVVHCERNTNIRIISARLATKRESGQYKGVH"
     gene            complement(2852..6385)
                     /gene="mfd"
                     /locus_tag="PXB27_001514"
     CDS             complement(2852..6385)
                     /gene="mfd"
                     /locus_tag="PXB27_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDL5346290.1"
                     /translation="MSLISLNLPKSDGNFQDHQTLGNLVGHSDTLAISQAARAFYGLT
                     VVVTPDTRTALRLEKALPQFAGLPVQLFPDWETLPYDNFSPHQDIISARLSALFELQQ
                     GRQQILLLPINTLMQKVCPPNYLANNVLLIKKGDRFSIQKLRLQLENAGYRAVEQVLE
                     YGEYAVRGSILDLFPMGAEEPFRLDFFDDEIDSIRTFDVDNQRTKTEIAEINLLPAHE
                     FPTDSNGIEHFRAKFREKFGEIRREPEHIYQQVSKGILNAGIEYWQPLFFEEMASLFD
                     YFPAQTLFITFADIQQKAEQFHQDTQNRYESHRVDPMRPLLPPSDLWFAIDEVNRWLK
                     GYPRLTITSEKIRKSAAKMNANVANLPDIAIQSSAKEPFSAFQQFRQKFDGNILFSVE
                     SEGRRETLLDLLAPLGVKPKQVNSLDEAQGLPFSLIISPLDQGFVVETGGHGTRPYDD
                     ELNTNGENDNNYVGAGPVPARNLAIICETDLLGERVQTRREKNRKAVNPDTLIRNLAE
                     LKIGQAVVHLENGVGRYGGLTVLDAGGIKAEYLVLHYANDAKLYVPVASLHLISRYIG
                     GADETAPLHKLGSEAWAKTRQKAAEKIRDVAAELLDVYAKRESQTGFAFAYDKAEFDQ
                     FSATFPYEETDDQKIAINAVISDMCQAKAMDRLVCGDVGFGKTEVAIRATFLAVMNHK
                     QVVILAPTTLLAQQHYENFKDRFANYPVNVEVLSRFKTAKEQKTILQNVAEGKVDILV
                     GTHKLLQDDVAFRDLGLLVIDEEHRFGVRQKEKIKQLRANVDILTLTATPIPRTLNMA
                     LNGMRDLSIIASPPARRLLIKTFVRQSDEMVIREAILREILRGGQVYYLHNDVATIEN
                     CAEKLAELVPEARIVIGHGQMRERELERVMSDFYHQRFNLLVCSTIIETGIDVPTANT
                     IIIERADKFGLAQLHQLRGRVGRSHHQAYAYMLTPHPKTLTKDAEQRLEAMSTIDNLG
                     AGFALATHDLEIRGAGELLGSEQSGQIESIGFSLYMDLLENAVKALQEGREPSLDEIT
                     QHQAEIELRVPALLPDDYLPDVNMRLSFYKRIASSENLQSLNDLKVELIDRFGLLPEA
                     TKNLFAITQLRLSAQTLGIKKIDAGIHGGYLEFKPTAQPDPMKFIALIQKQPAVYSFE
                     GAVKFKFRLALEDNRKRLEFVENLLREVVGG"
     gene            6546..7763
                     /locus_tag="PXB27_001515"
     CDS             6546..7763
                     /locus_tag="PXB27_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter"
                     /protein_id="HDL5346291.1"
                     /translation="MKNKILGSALMIAGTTIGAGMLAMPLTSAGMGFGATALLLVGLW
                     ALLAYTGLLFMEVYQTAPQKDVGVASLAEQYFGLIGRVLATFSLLVLLYALLAAYITG
                     GGSLLAGVMPEMADADMKLKISILIFTAVLGTFVVVGVKSVDGLTRVLFLGKIVAFIA
                     VLLMMLPKAKLENLTAIPLDNLLIISAIPIFFTAFGFHVIMGTINSYLDADIGKIRKS
                     IYIGTAIPLVAYLLWQLATHGVLSQNEFTTILTQDPTLNGLVKATSQITGSTILGEMV
                     RLFSVLALITSFLGVSMGIFEGVGDLLKRLNLPTNRLWLTIATFTPPILFALFYPNGF
                     IKALGYAGLLFAFYGMILPIGLAWKARRQYPNLPYRVIGGNLALVVALIAGIIIMVIP
                     FLIQAGYLPQAVG"
     gene            complement(7843..8349)
                     /gene="hutX"
                     /locus_tag="PXB27_001516"
     CDS             complement(7843..8349)
                     /gene="hutX"
                     /locus_tag="PXB27_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HDL5346292.1"
                     /translation="MISIEQQTALRNELAENPGQILEMLAAKYQCTLEEVMLNLPAEM
                     LKVTEGERFAEVLQQIHEWDDAITLISHTEDTIIEFVGKLPSGSISRGFYNFEHKEGG
                     GLQGHLRYENCAKIYLLDRPFMGKRTVSLIFINKKGNAMFKIFVGREKIGGALKEHQI
                     KALYQLIG"
     gene            complement(8404..10482)
                     /locus_tag="PXB27_001517"
                     /pseudo
     CDS             complement(8404..10482)
                     /locus_tag="PXB27_001517"
                     /inference="COORDINATES: protein motif:HMM:TIGR01786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
     gene            complement(10449..10832)
                     /locus_tag="PXB27_001518"
     CDS             complement(10449..10832)
                     /locus_tag="PXB27_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HDL5346293.1"
                     /translation="MVFTSYSIKSKKLCPTILALSVSSILSSQATYAEETTAELSEVN
                     VVAKEDVKASIEKKNKEIIQQELIQNNRDLVRYSPDVGIVNQGRHQKGFAIRGVEDNR
                     VGISIDGVSLFLIQKKIPFINAMVI"
BASE COUNT         3008 a         2499 c         2184 g         3318 t
ORIGIN      
        1 ttaaaaacgc tacgaacctc gtagaggtct caacaggtgc atattctatg gatttcagat
       61 ttaatgtcaa ataaatttgt aggaaatctg taaaaaatcg tttaattggt gaaaaataac
      121 gctttttgca aatttttcat caaatttgac cgcttgcagc cgccttattt gttgaaatca
      181 taatcagcaa atttccgaca aatatcaatt acaacttttg tggctttttc catcgtatca
      241 accgaaataa actcaaatcg tccgtggaaa ttttcgccgc cggtaaataa attcgggcaa
      301 ggcaagccca taaatgaaag gcgtgagcca tcagtaccgc cacggatcgg ttctaaattc
      361 ggctcaatgc cttgttcacg cattgcgttt tcggcaattt caaccaagta gccgtaatcg
      421 tccagcactt cacgcatatt gcgatattgc tcgacaatct cgacttttcc gcaatgttcg
      481 ccgtaaatct gttgcatctc tgccagcgta tcattcagca ttttgccgaa tgcagctaat
      541 tcatcacgct caaagctacg taaaatatag tgaatttccg ctttatcaat tccaccctca
      601 atgccgtgca acataataaa gccttcacgc ccttcgctgg tggcaggggt taaatgggga
      661 ggcagacggt gttggaactc gcaagctcgc tctaaggcgt tgatcatctt gccctttgcc
      721 gtgccggggt gaacgctacg cccgaagaac gtaacggtcg ctgcatcggc attgaaattt
      781 tcatattgca attcgcccag aggcccgcca tcaaaggtaa aggcgacatc ggcaccgaat
      841 gcgtttacat caaaatgatc tgcgcctcga ccgatctctt catcggtggt aaagccgatg
      901 cggatcttgc cgtgcggaat atgcggattg gcttgtaaat actcgatagc cgagagaata
      961 atcgcaatgc cggatttgtc gtctgcgcca agcaaagagg tgccgtcagt ggtgactaag
     1021 gtgtcgccaa tcagcttgtt taaattcggg aaatccgttg gcgaaagata atcgccactg
     1081 ccgtttagca aaatattgcc gccttgataa gcctcaatca cctgtggttt cacgttttca
     1141 ccattaaaat cgggcgaagt atcaacgtgg gcaatcagcc caagcaccgg cagggttttg
     1201 tcaatattgg caggcaagct ggcgaacaga tagccgttgg ggtcaagata aatctcgctt
     1261 agccccatcg cctctacttc cgttttcagc agatgaagca aatcccactg ctgttgcgag
     1321 cttggcacag tttggctttt cggatctgaa gttgtgtgaa tttgggtgta acgtagtaaa
     1381 cggttaagaa gggattgttt cattgttttt ccttactata gtttagtgta accaattctc
     1441 caatttcaga tcaggtacca ttttaaatgc ctgatcacta gaaactaaaa tacactcttc
     1501 agcaagagcg tgactagcaa tcatttgatc ctgcacgccc attactttgc catttttctc
     1561 catctctgcc cgtaactttc cataaagttc agcacaacga taattccaat ctaaaacctt
     1621 tacactcgat aaaaaataat ctaaaaattg agaaagctgc ccattatttc ttttcgccac
     1681 accataaacc aattcagcag cagttacaga tgaaatacaa agcctctcag gattaatgga
     1741 cttcaatttc gccaccacat ttgcatttcc acgaaaaaaa tagcttacag tattcgtatc
     1801 taacatatac atttaagcta cctcaaaagg atcccgttgc tcaaaggatt ggtttctctc
     1861 ttttgtatct aaaaatgttt catcacatac aacagcaggc aatagctcaa acaatcgttg
     1921 ccaatgctcg gtatttctcg atctcaatga taataaaata tcgccatttt cttttttaga
     1981 aacatagact tgctctgcat caaactcaaa ttcaaccggt aaacgcactg cttggttccg
     2041 cccgttacgg aataatctcg caattttttc catcttactt taccctccga tagatagtgt
     2101 atatgccaaa gtatatgcct attttataca actggcaaga taacccattc tcaaatactc
     2161 aataacatac tgatttcaaa tcataatatt accaactaat agtcggttta cgtttctgct
     2221 caacgcaatc tttcaaataa agattgatca acgtttggta aggcaaattc acctcttcgg
     2281 caagttgttt aaaatagctg ataacatcgt cttcaatccg aatagtaacc acacttttgc
     2341 tttttagccc ttttgcgtaa gggtttttca ctgcattaga aaaatcgtat tcatctctca
     2401 tttaatgtac tcctttatat tgcccacttt cgcgttttgt tgccaaacgt gctgaaataa
     2461 tccgaatgtt ggtatttctc tcgcaatgca caaccacaag tagattatct ttattgctac
     2521 gcccgagtag caaaaaacgc tcttccctag atgagtgatc tggatccggg attaacatcg
     2581 cattattatc ataaaatacg cttaccgcct cgataaaact aactccgtgt ttttgttgat
     2641 tacgaagagc tttctcacta tcccattcaa aaccattaat ttcttccatt gccagtttac
     2701 tcctttttga ttgtaaataa aatatattta catctatttt tttaagttat aaacagccta
     2761 gatgaaatta ctggattcgc aataaacaaa aacgattttt acgatacaga tcgcaaaaat
     2821 cgtttttcaa gattgacaac cttaggaggc tttatccgcc cacaacctcc cttagcaaat
     2881 tttccacaaa ttctaaccgc ttgcggttgt cttcgagtgc taagcggaat ttgaacttca
     2941 ccgccccttc aaaactgtac accgctggtt gtttttggat taaggcgata aacttcattg
     3001 ggtcgggctg ggctgtcggt ttaaattcaa gataaccgcc gtgaatgccg gcatcgattt
     3061 tcttaatccc gagcgtttgt gccgacaagc gaagttgggt tatcgcaaac aggtttttgg
     3121 ttgcctctgg gagcaagccg aagcgatcga tcagctccac tttcaagtcg ttcaggcttt
     3181 gcagattttc cgagcttgcg atccgcttgt agaacgacag acgcatattc acgtcaggca
     3241 gataatcgtc cggcaataag gccggtacac gcaattcaat ctccgcttgg tgttgggtga
     3301 tttcatcgag gctcggttct cgcccttctt gcaaagcttt gaccgcattt tcgagcaagt
     3361 ccatatagag cgaaaaaccg atgctttcaa tctgtccgct ctgctccgaa ccgagcaatt
     3421 cgcctgcacc acgaatttct aaatcgtggg tcgccaacgc aaaaccggcc ccgagattat
     3481 caatggtact catcgcctcc aagcgttgct cggcatcttt ggtgagcgtt ttcgggtgcg
     3541 gtgtgagcat ataggcgtag gcttggtggt gcgagcgtcc gacccgtcca cgcagttggt
     3601 gcagttgtgc cagcccgaat ttgtccgccc gttcgataat aatggtattg gcagtcggca
     3661 cgtcaatccc cgtttcaata atggtggagc aaaccagcag gttaaagcgt tgatgataga
     3721 aatcgctcat cacccgctcc aattcacgct cacgcatctg cccgtgtccg atcacaattc
     3781 ttgcctctgg cacgagttca gccaatttct cggcacaatt ttcaatggtt gccacatcgt
     3841 tatggaggta gtaaacctgc ccaccacgca gaatttcacg caaaattgcc tcacggatta
     3901 ccatttcatc actttgtcgc acaaaggttt tgattaaaag tcggcgagcc ggcgggctgg
     3961 cgataatcga gagatcacgc attccgttga gtgccatatt cagcgttctt ggaattggcg
     4021 tggcggtgag ggtcagaata tccacattgg cacgcagttg tttgattttc tctttttggc
     4081 gtacgccgaa acggtgttct tcatcaatca ctaaaagccc aagatcacgg aacgctacat
     4141 cgtcttgcaa cagtttgtgc gtgccgacca aaatatccac tttgccctcc gccacatttt
     4201 gcaaaatcgt tttctgctct ttggcggttt taaagcgaga cagcacttcc acattcacag
     4261 ggtaattggc gaaacggtct ttgaaattct cataatgctg ttgggcgagc aaggtagttg
     4321 gggcaagaat aaccacctgt ttatggttca tcactgccaa gaaagtcgct cgaatggcca
     4381 cttcggtttt gccgaagccg acatcgccac acaccaatct atccatcgct ttggcttggc
     4441 acatatcgct aattacggca ttgatggcaa ttttctgatc gtccgtttcc tcataaggga
     4501 aagtggcact aaactgatca aattccgctt tatcgtaggc aaacgcaaaa ccggtttggc
     4561 tttctcgttt ggcataaaca tcaagcaatt ctgccgccac atcacggatt ttttccgctg
     4621 ctttttggcg ggttttcgcc cacgcttcac tgcctaattt atgtagcggc gcagtttcat
     4681 ccgccccacc gatataacgg ctgattaaat ggagcgaggc aaccggcaca tagagtttgg
     4741 catcgttagc ataatgtagc acgagatatt cggctttgat cccaccagcg tccagcacgg
     4801 ttaagccacc gtaacgcccc acgccgtttt ccaaatgcac caccgcctgt ccgattttga
     4861 gttctgctaa attacggatt aaggtatcag gattgacggc tttgcggttt ttctcacgcc
     4921 gtgtttgcac acgttcgccc agcaggtcgg tttcgcagat gatggcaagg ttgcgggcgg
     4981 gcacaggacc cgcccctacg tagttattat cattttcgcc atttgtattt aattcatcat
     5041 cgtagggacg ggtcccgtgc ccgcccgttt ccaccacaaa cccttgatca agcggtgaaa
     5101 taatcaggga aaatggcaaa ccttgtgctt catctaatga attgacctgc ttcggcttca
     5161 ccccaagcgg agcaagcaaa tccagcaagg tttctcttcg cccttcgctc tccaccgaaa
     5221 acagaatgtt gccgtcaaat ttttggcgga attgctggaa agcagaaaac ggctctttgg
     5281 cagaggattg aatagcaata tccggcaagt tagccacatt tgcattcatt ttcgctgctg
     5341 atttacggat tttctccgaa gttatcgtta aacggggata gcctttcagc cagcgattta
     5401 cctcatcaat cgcaaaccag agatcactcg gcggcagtag cggtcgcatc ggatccactc
     5461 ggtggctttc atagcggttt tgggtatctt ggtgaaattg ttcagctttt tgctgaatat
     5521 cggcgaaagt gataaacagc gtttgggcag ggaaatagtc aaacagactt gccatttcct
     5581 caaaaaacaa cggctgccaa tactcaatcc ctgcatttaa aatgcctttg ctgacttgct
     5641 gataaatatg ctccggctca cggcggattt caccgaattt ttcacggaat ttggctctaa
     5701 aatgctcgat cccgttgcta tcagtcggga actcgtgtgc cggcagtaaa ttaatttcgg
     5761 caatttccgt tttggtgcgt tggttatcca catcaaaagt gcggatactg tcgatctcat
     5821 catcgaaaaa atcgaggcga aacggctctt ctgctcccat cgggaatagg tctaaaatcg
     5881 agccacgcac ggcgtattca ccatattcca acacctgttc caccgcccga tagccggcat
     5941 tttccagttg taaccgcagt ttttgaatcg aaaaacggtc gcctttttta atcaacagca
     6001 cattgttagc gagataattc ggcgggcaga ccttctgcat taaggtgttg atcggcaaca
     6061 gcaaaatctg ctgtcgccct tgttgcagtt caaacagggc agaaagtcgg gcggagataa
     6121 tgtcttggtg cggcgaaaaa ttgtcataag gcaaggtttc ccaatcgggg aacagttgca
     6181 ctggcagccc tgcaaactga ggcaaggctt tttccaagcg taacgccgtg cgggtgtcgg
     6241 gtgtgacaac caccgtcaag ccgtaaaagg ctcgggcagc ttggctgatc gcaagggtat
     6301 cggaatgccc gacaaggtta ccgagggttt gatgatcttg aaaattgccg tcagatttcg
     6361 gcagattaag tgaaattaaa gacatacaag cggtctgttt taaataaaaa attgcaaatt
     6421 gttgcttagt ttactaaagc acggctgatt gtgcaaaata agcggagaaa ttatgttatc
     6481 tagatcgcat tgcgaaaaaa ttctgataaa atgttcccct tttttaactc ttactcacac
     6541 atattatgaa aaataaaatt ttaggaagtg cattaatgat tgcaggcact accattggtg
     6601 caggaatgct tgcaatgcca ctcacctctg caggaatggg ttttggggca accgcattac
     6661 tcttagtcgg attatgggct ttattggcct acaccggttt attatttatg gaagtttatc
     6721 aaaccgctcc gcaaaaagat gttggagtgg caagccttgc cgagcaatac tttggcttaa
     6781 tcgggcgtgt attagccact ttcagtttgc tcgtactact ttatgcccta cttgcggctt
     6841 atattaccgg tggtggttca ctgttagccg gtgtaatgcc tgaaatggca gatgctgata
     6901 tgaaactgaa aatcagcatt ttaatcttca ctgcggtatt aggtacattt gtggtagtcg
     6961 gcgtgaaatc ggttgatggt ttaacccgag tactgttttt gggaaaaatt gttgctttta
     7021 ttgccgtgtt gctgatgatg ctgcctaaag cgaagctgga aaacttgaca gcaattccat
     7081 tagataactt actcattatt tctgctatac ccattttctt cactgcgttc ggtttccacg
     7141 ttattatggg aacaattaat agctatttag acgcagatat cggcaaaatt cgtaaatcta
     7201 tttatatcgg gacggcaatt ccgctagtgg cttatttatt gtggcagctc gctacacacg
     7261 gggtattaag ccaaaatgaa ttcaccacaa ttttgacaca agatccgacc ttaaacggct
     7321 tggtgaaagc cactagccag attaccggta gtacgatttt aggcgaaatg gttcgcttat
     7381 tctcagtgct tgcattgatt acctcatttt taggggtttc gatgggaatt tttgaaggcg
     7441 tgggcgattt gttaaaacgc ttaaatctac cgactaaccg tttatggctg accatcgcca
     7501 cctttacacc gccaattctg ttcgctttat tctatccgaa cggctttatt aaggcattag
     7561 gttacgccgg tttactcttt gcgttctatg gtatgatttt acctatcggc ttagcgtgga
     7621 aagcacgtcg ccaatatccg aatttacctt accgtgtgat tggtggtaat ttggcattag
     7681 tggttgcgtt aattgccggt attatcatta tggtcattcc gtttttaatt caggctggct
     7741 atttaccgca agcggtaggc taatattgaa aagggctacg attcaatatc gtagcccttt
     7801 ctataagtta caagcggtca tttttcgatg actttttaca gattagccaa tcaactgata
     7861 cagcgcctta atttgatgct cttttaatgc cccgccaatt ttctctctgc cgacaaaaat
     7921 cttaaacatt gcgttgcctt ttttattaat gaaaatcagt gaaacagtac gttttcccat
     7981 aaaaggacga tccaacaaat aaattttggc acaattttcg tagcgtagat gcccttgtaa
     8041 gcctccgccc tctttatgtt cgaaattgta aaatccacga gaaattgaac cgcttgggag
     8101 ctttcccaca aactcaataa tggtatcttc cgtatgcgaa atcagcgtaa tcgcatcgtc
     8161 ccattcgtgg atttgctgta gcacttcggc aaaacgttcc ccttcggtta cttttagcat
     8221 ttcagccggt aaattcagca ttacctcttc cagcgtgcat tgatattttg ccgctaacat
     8281 ttctaaaatt tgccccggat tttcggctaa ttcattgcgt aaggcggttt gttgttcgat
     8341 tgatatcatt aaatgttcct ttaagtaaag agagtttctt agctctctaa gtaaatttgt
     8401 aaattagaaa cggtattcaa aattagccga gaagttacgt ccaggttcat aatagcgttg
     8461 taatccagga tagccaccgt aagttgcatt ttcacgtact ccaacagaat taacgattcc
     8521 acctgttgta tttaaaccac gtaaagtatc ccaagtatgg tatttacggt taaacaaatt
     8581 ataaataccg gcacgtaatg taatattctc agttggttta tagaaaccgt ataaatccac
     8641 aacaaaggct ttagcattta agtgcttcca ggtatctaaa cctgtctcgt gagaatattc
     8701 agtacaacga atgtcatatt catcccccca ataagggtaa tacggatttg gcgttctatc
     8761 ctctttaagg catttttccg gtaatgtttt taaatatttt gcatctttcg cctttttagc
     8821 ccccaagaat gtgaagcgag aaaatactcc ccatttacct tcaggttgtt cataatctaa
     8881 gccaactacg gctttaatcg gctgaataga aagtaaatct gccccgttag acatactacc
     8941 tttgctgtaa ccgaaggctc caaatagctt ccagccttca ggtattggca tagctgaatt
     9001 taaattccat ttaccactca cttctagccc gtagattttc gcagaatcaa tattttgcat
     9061 ttgctgtacc gggcgccaaa actctctacc atatgcttgg taaacatccc aggtttcttg
     9121 ctcccataaa aaatctttat atttagtatg gtggaaatta actgagagca gcccaacatt
     9181 gccactacct tgcaaactta aattttggct taaactacgt tccgctttta ggtttggatt
     9241 ggacatccaa gctcctgcag cattatcacg gtaatcaaaa tacatttctg atgcatttgg
     9301 aatacggaag cctgtcccaa tgttatatcc tactttccat gcctcagtca gatgtttttc
     9361 taaaccggta acccaagtta gttgattaaa tttcgcattg tcagttttta cgcagttacg
     9421 acaaacaaga ccattgagtt cttgttgttc atacttcgcc caatcataac gaatgcctag
     9481 cgttgcaatc caatcatgcg ttaaatcaat cttatcgtgc aatgaaaaag tgtgctgttt
     9541 cgttttaatt gggtacatca tcgtagaatc gggagaattt acccaaacac cggataaata
     9601 aaccgagtcc ttatgtaata catcgaaatc tctctctgca gtagaggctt tgaaagatag
     9661 ctgatgagtc catttaccaa attctaatgc ttggctgtct aaacggaaat ttaagcgttt
     9721 caattcagta ttaaaaatac gaatattatt gtcacttggt tttctttgtt tagcagggct
     9781 ccagtctgtt gcatcttcag cgcgtgtacc ttcatagtta taagcagatg tttcagtatc
     9841 ttgataatct gcatctatct tcgctagaga tatccatttt gactctggga aatactcata
     9901 aaaaacattt actgtatcac gtttactacg gtcttccgct tcacgccaaa aagaagtaat
     9961 agtaacggct gaatcttcga taatataatt tttattattt tgcccactgt aagaaacacc
    10021 aacgcggtga ttatcattaa atcgataagc aaatttggct aaataattat gatgattatg
    10081 agtagaatca tcaggagttt gtttactact gcgtaaagag cgagtttgta gagaatcttc
    10141 aggaagtgta taacctcctg cacttttcat ttcatgccca tggcgatttg aataaaccaa
    10201 tatcgcctca gctttctcac ctaaatatcc aacaccgaat gtattcaacc attcattatt
    10261 acgagtacca tatcctgtgc gataaaaagc accaaattta ttaccgttac gaacaatatc
    10321 ataaggctca agcgtccgat aattaactcc gccgcctaaa ttaccactac cttgattgaa
    10381 agaatctgca cctttagata cttcaatagt acgagctaat tccggatcta tagattggcg
    10441 agatgtattt aaattaccat agcgtttata aagggaattt tcttctgaat caggaaaagc
    10501 gagacaccat caatacttat tcctacacgg ttgtcttcca ctccacgaat agcaaatcct
    10561 ttttgatgtc taccttggtt aacaatgcca acatcagggc tataacgaac taaatctcga
    10621 ttattttgaa tcaactcttg ctgaattatc tctttatttt tcttttctat agaagccttc
    10681 acatcttctt tagcaacaac atttacttca gataattccg ctgttgtttc ttccgcatag
    10741 gtagcttgtg aagacaaaat gctactaacg cttaaagcca atatagtcgg gcacaatttt
    10801 ttagacttta ttgaataaga ggtaaacacc atgattctcc ctagatagat ttttaatata
    10861 ctttattatt aataagatgt attatcagtt acatcacatt tatagtcaat aatgattctc
    10921 atatggattg aaagttttca ctctttatag taatctaaaa aaacactatt taaattgagg
    10981 tattttatga caaccaaccg tcaagaggt
//
LOCUS       DANLUQ010000076        10893 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.076, whole genome shotgun sequence.
ACCESSION   DANLUQ010000076 DANLUQ010000000
VERSION     DANLUQ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10893
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.076"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            139..885
                     /locus_tag="PXB27_001519"
     CDS             139..885
                     /locus_tag="PXB27_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL5346294.1"
                     /translation="MQLRHKQIMEYIQQFDEANVQELAEHCGVSIETIRRDLNKLDKD
                     GLLHRTHGGAVSHKKRDIGRSFTTRQRLNSEAKRSIAENVLEYLQPESVIALDASSTS
                     WNVAQRIPNIPCTVVSSSMRIIYSLSHKPHIKTIATGGIYSEKYDAFYGPLSEQLLTR
                     LKIDLAILSCTGIADGAIWESNEINISLKRKLIANSKQVFLLADHSKFERKDLFRIED
                     LSCIDKLFINQPPPDFLKNYCLAHQIEIII"
     gene            1081..2844
                     /gene="fucI"
                     /locus_tag="PXB27_001520"
     CDS             1081..2844
                     /gene="fucI"
                     /locus_tag="PXB27_001520"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005619331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HDL5346295.1"
                     /translation="MTTLKSTPVKIGIRPTIDGRRLGVRESLEDQTMNMAKAVAQLIQ
                     SEVRHPNGDFVECVIADSTIGGVAEAAACAEKFKRENVGAVITVTPCWCYGSETIDMD
                     PHMPKAIWGFNGTERPGAVYLAAALAGHSQLGLPAFSIYGTEVQEADDQSIPDDVREK
                     LLRFARAGLAVATLRGKSYLSIGSVSMGIAGSIVNQQFFQEYLGMRNEYVDMTEIKRR
                     LDRHIYDAEEFKLAMSWVKTYCKEGIDVNAPENQRSPEEREKLWEDVVKMTIIARDLM
                     VGNPRLAELGYSEEALGHNAIAAGFQGQRQWTDHLPNGDFMEATLNSTYDWNGVRAPY
                     IMATENDSLNGVNMLFGNLLTGQAQIFADVRTYWSEDSVERVTGWRPESGFIHLINSG
                     SAALDGTGQHTDRAGKPTIKPAWEVTEEDGKRCLENTCWCPAVHEYFRGGLSSQFLTK
                     GGMPFTMHRINLIKGIGPVLQIAEGWSIDLPENVHDILNKRTNETWPTTWFVPRLTGK
                     GAFTDVYSVMANWGANHCVATFGHVGADLITLASMLRIPVCVHNVEDKDVFRPSAWNG
                     FGQDKEGQDYRACANFGPLYK"
     gene            3038..4471
                     /gene="fucK"
                     /locus_tag="PXB27_001521"
     CDS             3038..4471
                     /gene="fucK"
                     /locus_tag="PXB27_001521"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HDL5346296.1"
                     /translation="MPISLIFDCGATNLRTIAINQAGEIVAAHHLPNNTQPDVESPEF
                     HIWDIEEIWSKLMTCAKQTLSQLSEQQRQEIVGISVTTFGVDGALFDKNGKQLYPIIS
                     WKCPRTLPIMENLANYLDVEALYQRNGVGHYSFNTLFKLLWLKENKPEIYQQAESFLF
                     ISSILTYLLTGVQTTDRTMAGTSMMTNIENDSWDKTVLDLLGLTENHFPPMKSAGEVV
                     GTLKGEISQELGLSGQIPVISCGHDTQFAIFGSGAGYNQPVLSSGTWEILMVRTPKAK
                     PQWQFVQNGLTIEFDSQSGYFNPGVQWVASGVMEWFGKRFFSDIANTEHYYRTMIEEA
                     AEVPAGSNGVKLVGNFDGTTAKTGSIIGLSMHITRGEIYRAGLEYMAHRLKEGLDVLQ
                     EVGHFNAESLICVGGGSKNALWNQIRADVLNRPIDVVDFPESTVLGAAMFTFTGAGVF
                     ENPNAAQQAMKPNVKRVEPSSNSQFYK"
     gene            4546..4977
                     /gene="fucU"
                     /locus_tag="PXB27_001522"
     CDS             4546..4977
                     /gene="fucU"
                     /locus_tag="PXB27_001522"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012341412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HDL5346297.1"
                     /translation="MLKGIHPAISPDLLKVLAEMGHGDELVLSDAHFPAHSIHQNVIR
                     ADGLSVATLLEGISALFEFDQYVEAPLAMMQAVKGDTLDPSVEERYLNAIKKVNGTAP
                     KIERVERFAFYDRAKNAYAVVITGELAKYGNIIIKKGVTPV"
     gene            5037..5690
                     /locus_tag="PXB27_001523"
     CDS             5037..5690
                     /locus_tag="PXB27_001523"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HDL5346298.1"
                     /translation="MDRKILARQIIDTCLEMTRIGLNQGTAGNVSVRYKDGMLITPTG
                     TPYEQMTEDSIVFVDGNGKHEEGKLPSSEWQFHLAVYEARPELNAVVHNHAKNCAAVS
                     ILGEPIPAIHYMIACTGTNHIPCVPYAPFGTHQLADYVREGIKHSKAILLAHHGLITA
                     DKTLEKALGIAHEVEVIAEWYLKLLATGKPIPTLNKEQMDIVLGKFKSYGSWVESEQ"
     gene            5752..7281
                     /locus_tag="PXB27_001524"
     CDS             5752..7281
                     /locus_tag="PXB27_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL5346299.1"
                     /translation="MQNEVILTMKNISKSFAGVNALKGVELTLRKGEVHALMGENGAG
                     KSTLMKCLLGVYQADEGEIIYKGQPVRFESVLEAQKAGISMIFQELNLIPHLTVAENI
                     FFAREPLKHGLVDKDKMVEESAKLLNFFEIDVDPNDEVRMLSVAKQQMVEIAKALSFD
                     VEVLIMDEPTSALTEKEIDKLFELVDRLRAKGVSIVYISHRMEELKRICNHITIFRDG
                     TYVSNHKFNEITMDEIITKMVGRSLDNHFPPKTAVVTNDIILSIMNAERKGVFEPLNF
                     DLRKGEILGITGLVGAKRTELARAIFGADPLDSGEIFVHENKVSIKDPSDAIKAGIAY
                     LSEDRKLNGVAVRMSIRENITMASMDKVANQIGVISYDEEEKASKTFIDKMEIKTPTI
                     EQKVQNLSGGNQQKVVIGKWLFREAKVMIFDEPTRGIDVGAKYAIYQLLDELAANGVG
                     VIVISSELPEVLGVSDRIIVMREGRMTGMLETKKTNQEEIMHYATGVKNMFAREYGVE
                     K"
     gene            7278..8276
                     /locus_tag="PXB27_001525"
     CDS             7278..8276
                     /locus_tag="PXB27_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5346300.1"
                     /translation="MNEKQKEMLRKMAALAGLVLLIIFFSVTNEYFFTSNNIMTVGLQ
                     TSTIALIGIGATCVILTGGIDLSTGSVVALSGVAAAMIVNAGVPVPLGMILGILVGGI
                     CGLINGILVTRMKLPPFIATLGMMMVARGLALYVTNAAPVSGMPESFANLGNGALFKI
                     VEEGANGLPKVVFAGIPYPVIIMIFITVLFTFALSKLKVGRYLYAIGSNEEAARLSGI
                     KTNIVKIYAYVASGLLSGLTGVILASRLVTAQPNGGVSYELDAIASAVVGGTSLMGGV
                     GTIPGTLIGSFIIGVLRNGLNMNGVSSFVQMIVIGLVIIVAVSLDQLRQSKKAGKK"
     gene            8300..9247
                     /locus_tag="PXB27_001526"
     CDS             8300..9247
                     /locus_tag="PXB27_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5346301.1"
                     /translation="MKKSALFTATLFGLSTLFAGSAMAKSDEIAVIVKSANSTFWQNV
                     RKGAETAGKDIGGQYKVTFQGPEAETAIDAQVNMVDNAVNRGVAGIVLAASDPVALVP
                     AVKKAYESGIPVVLIDSGINSDGKYYQSFLATDNRAAGKLAAEKLLAKVKGGKVAVMS
                     FTQGAASAIERTGGFIDEIKAKADYKLVGPYYSDSDMTKALNQTEDVLGSNPDLAAIF
                     GANEPTAVGMARAVKQKGFAGKIVAVGFDGNSDLQNFVRDGTLDGIVVQSSYQMGYKG
                     VDTIGKLIKGEKVEKVIDTGVVYVTKENIDSEEAKGVLY"
     gene            9303..9554
                     /locus_tag="PXB27_001527"
     CDS             9303..9554
                     /locus_tag="PXB27_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HDL5346302.1"
                     /translation="MSNTLTDKALVVIAALGGRENIVDVYACITRLRVEVKNTDIVEW
                     DKIKSVGALGVVTSGSVIQSIFGSDAEAYRDEIKRILNI"
     gene            9683..10834
                     /gene="fucO"
                     /locus_tag="PXB27_001528"
     CDS             9683..10834
                     /gene="fucO"
                     /locus_tag="PXB27_001528"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HDL5346303.1"
                     /translation="MANRLILNETSYHGKGAIQNIVHEVTSRSFKKALVVTDKDLIKF
                     NVAQKVTALLDAAGLPYEIFDEVKANPAVDVIKAGVAKFKASSADYLIAIGGGSPIDS
                     AKAIGIIINNPEFSDVLSLEGVAPTHQKCVPIIAIPTTAGTAAEVTINYVITDEAKKR
                     KFVCVDVHDVPAVALVDPDMMESMPKSLAAATGMDALTHAIEGYITKAAWELTDALHL
                     KAIELIGRSLRGAVEKNPKDVEAMALGQYVAGMGFSNVGLGAVHSMAHPLSAYYDTPH
                     GIANAVLLPYVMEFNKNYTGEKYREIARAMGVKGVDEMSQEEYRNAAIAAVQQLSQDV
                     GIPPKLHQIGVKEADLAALSVDAFNDVCTGGNPRDCTAEELLEVYRIAY"
BASE COUNT         3269 a         2274 c         2478 g         2872 t
ORIGIN      
        1 caaattttac aaaaagttcc ctaaatttaa ccgcttgcaa cacaaaatgt gatcgccttc
       61 taaaaatcaa atttgcttgc tcgacagtaa cgctacaagc ggttaaattt ggagaaaaat
      121 ttacaaaaag gatagattat gcagctgcga cacaaacaga taatggaata tatacaacag
      181 tttgatgaag ctaacgttca agaattggcg gaacattgtg gggtttcgat tgaaactatc
      241 cgccgagatt taaataagct agataaagat ggattattac atcgcactca cggtggagct
      301 gtgagccata aaaagcgaga tattggtcgc tcatttacca cccgacaacg gctgaacagt
      361 gaggcgaaac gcagtatcgc agaaaatgtg ttggaatatt tacaaccaga atcggtgatt
      421 gctttagatg ccagctcaac ctcttggaat gtggcacagc gtattccaaa tattccttgc
      481 accgttgtca gcagttcaat gcggattatc tacagccttt cacacaaacc gcacattaaa
      541 acgattgcca ccggtggtat ttattccgaa aaatacgatg ccttctatgg tccattatcc
      601 gagcagttac tgacccgttt aaaaattgat cttgccattc tttcttgtac aggcattgcc
      661 gatggggcga tttgggaatc aaatgaaatt aatatttccc ttaaacgtaa attaattgcg
      721 aacagtaaac aggttttttt attagccgat cacagtaaat ttgagcgtaa agacttgttt
      781 cgtatcgaag atttatcttg cattgataaa ttatttatca accaaccccc accggacttt
      841 ctcaaaaatt attgcttagc acatcaaatt gaaatcatta tttagtgatt tctgcccgat
      901 tttattcaga aataaaacaa atcgggcatt cttttttcac tcatttttac caaaatcccc
      961 cattttttgt gatcgcactc acaaaattta aactaaattc gcatttttct tcataactca
     1021 tattgcgtat gcataataaa ttcaatcgca aattttttta accaatcggg agaactattt
     1081 atgacaacgt tgaaatctac ccctgtaaaa attggtatcc gcccaaccat tgacggtcgc
     1141 cgtttaggag tgcgtgaatc acttgaagat caaacaatga atatggcaaa agccgttgca
     1201 caattaattc aatctgaagt ccgccaccca aatggtgatt tcgttgaatg tgtgattgct
     1261 gattccacca tcggtggtgt tgccgaagct gcggcttgtg cagaaaaatt caaacgtgaa
     1321 aatgtcggag cggtgattac cgtaacccct tgctggtgct acggttctga aaccattgat
     1381 atggacccac atatgccgaa agccatttgg ggctttaacg gaacagaacg cccaggtgca
     1441 gtttatttag ctgctgcttt agcaggtcat agccaattag gtttaccggc attctccatt
     1501 tatggcacag aagtacaaga agctgacgat caatctattc cagacgatgt gcgtgaaaaa
     1561 ttactccgct ttgcccgtgc cggcttagca gtggcaacct tacgaggaaa atcatattta
     1621 tctatcggct ctgtttcaat gggtattgcc ggttctatcg taaaccaaca attcttccaa
     1681 gaatatttgg gtatgcgtaa cgaatatgtg gatatgaccg aaattaaacg ccgtttagat
     1741 cgccatattt acgatgccga agaatttaaa ttagcaatga gctgggtgaa aacctattgc
     1801 aaagaaggta ttgatgtcaa cgcaccggaa aatcaacgca gcccggaaga gcgtgaaaaa
     1861 ctctgggaag acgtggtgaa aatgaccatt attgcccgtg atttaatggt gggtaatcct
     1921 cgcttagcag agctaggcta tagtgaagaa gccttagggc ataatgcgat tgctgccggt
     1981 ttccaagggc aacgccaatg gacagaccat ttgccaaacg gcgattttat ggaagctacc
     2041 ttgaattcga cctatgactg gaacggcgtt cgcgcaccat acattatggc aactgaaaac
     2101 gacagcttaa atggcgtgaa tatgttgttc ggtaacttat taaccggtca ggcacaaatc
     2161 tttgccgatg tgcggacata ctggagcgaa gactcggtag aacgtgtcac cggctggcgt
     2221 ccggaaagcg gttttatcca cttaatcaac tccggctctg cggcgttaga cggaacaggt
     2281 cagcataccg atagagcagg caaaccaact attaaaccgg cttgggaagt gactgaagaa
     2341 gacggtaaac gttgtttaga aaacacctgt tggtgtccgg cagttcacga atatttccgt
     2401 ggtggtttat cttcccaatt cttaaccaaa ggtggaatgc cgtttacgat gcaccgtatc
     2461 aacctgatca aaggtatcgg gccggtttta caaattgccg aaggttggtc tatcgacttg
     2521 ccggaaaatg tacacgacat tttaaacaaa cgcaccaatg aaacctggcc gaccacttgg
     2581 ttcgtacctc gcttaaccgg aaaaggcgca tttaccgatg tgtatagcgt aatggcgaac
     2641 tggggtgcaa accactgtgt ggcaaccttc ggacacgttg gggcagactt aattaccctc
     2701 gcctcaatgc tccgcattcc ggtttgtgtg cacaatgtgg aagataaaga cgtattccgc
     2761 ccaagtgcgt ggaacggttt cggtcaagac aaagaaggtc aagactaccg tgcttgtgct
     2821 aacttcggac cattatataa ataatacaca atcaccctct ccccttgtgg gagagggaca
     2881 gccaagcaat tcgttcagaa ttgaatggca gggagagggc tacaagcggt cgcttttctc
     2941 tcaaaattta caaatccctc tctctgatta aattgctaaa cgcaatttaa tctatctctc
     3001 taaaagggag atattaggat aagtaaagga gataacaatg ccaatttctc ttattttcga
     3061 ctgtggtgcc accaacctcc gtacgattgc gattaatcaa gcgggtgaaa ttgtggcagc
     3121 ccatcatcta ccgaataaca cacaaccaga tgtggaaagc ccagaatttc atatttggga
     3181 tattgaagag atctggtcga aattaatgac ctgtgccaaa cagacgcttt ctcaactttc
     3241 agaacaacaa cggcaagaga ttgttgggat ttcagtaacc acctttggcg tggacggtgc
     3301 attatttgac aaaaacggca agcagcttta tccgatcatt tcttggaaat gcccgagaac
     3361 cctaccgatt atggaaaacc ttgccaatta tcttgatgtt gaggcgttat atcaacgtaa
     3421 tggcgttgga cattatagtt tcaacacctt attcaaatta ttgtggttaa aagagaacaa
     3481 accagaaatt tatcaacaag ctgagagttt cttattcatc tcatctattt tgacttacct
     3541 tcttacaggc gtgcaaacaa ccgaccgcac aatggcggga acgtcaatga tgacaaacat
     3601 cgaaaacgat tcgtgggata aaacagtttt agatttatta ggcttaaccg aaaaccattt
     3661 tcctccaatg aaaagtgcag gagaagttgt gggaacatta aaaggagaaa tttcccaaga
     3721 gttaggttta agcggtcaaa ttccagtaat ttcttgcgga cacgatactc aatttgccat
     3781 ttttggctca ggagctggct ataaccaacc tgtgttaagt tcaggcactt gggaaatttt
     3841 aatggttcgt accccaaaag caaaaccaca atggcaattc gttcaaaacg gcttaaccat
     3901 tgagtttgat agccaatctg gttatttcaa cccaggtgtg cagtgggtag cttcaggtgt
     3961 gatggagtgg tttggcaaac gtttcttctc tgatatagcc aatactgaac attactaccg
     4021 cacaatgatt gaagaagcgg cagaagtgcc tgctggctca aacggtgtga aattagtcgg
     4081 caattttgat ggcacaacag ctaaaacagg ttcaattatc ggcttatcta tgcacatcac
     4141 tcgtggcgaa atttaccgtg caggcttgga atatatggct catcgtctaa aagaagggtt
     4201 agatgtatta caagaagtcg gtcattttaa tgctgagagt ttgatttgtg tgggaggtgg
     4261 ttctaaaaac gctttatgga atcaaattcg agccgatgtc ttaaatagac cgattgatgt
     4321 agtggatttc cctgaaagta ccgtattagg cgcggcaatg tttactttta caggtgctgg
     4381 tgtatttgaa aatccaaatg cggctcaaca ggcaatgaag ccaaatgtga aacgggttga
     4441 gccatcgtca aatagtcaat tttataaata aagtttctcc cctctctctg atttgaggtt
     4501 cattcagaac atcaaatcag agagagggta aaaaaggagt tcaaaatgtt aaaaggtatt
     4561 cacccggcaa tttctccaga tttattaaaa gtcttagccg aaatggggca cggcgatgaa
     4621 ttagtgcttt ctgatgcaca cttccctgct cactctattc atcaaaacgt cattcgtgca
     4681 gatggcttaa gtgttgccac cttattagaa ggcatttctg ctctgtttga atttgaccaa
     4741 tacgttgaag ccccacttgc aatgatgcaa gcagtaaaag gcgacacgct cgatccaagc
     4801 gttgaagagc gttacctcaa tgcaattaaa aaagtaaacg gaacagcccc gaaaatagag
     4861 cgtgtagaac gcttcgcttt ctatgataga gccaaaaacg cttatgccgt tgtcattaca
     4921 ggcgagttag ccaaatacgg caacattatt atcaaaaaag gcgtaacacc ggtttaatta
     4981 caagcggtta aaaaagcaga attttttaca aatataaaat taaaaggagt tcaacaatgg
     5041 acagaaaaat actggcgaga caaatcattg atacttgtct tgaaatgacc cgaattgggt
     5101 taaatcaagg tacagcaggc aatgtgagcg tacgttataa agacggtatg ctcattacgc
     5161 caacaggtac accttatgag caaatgaccg aagattctat cgtctttgtt gatggcaacg
     5221 gcaaacacga agagggaaaa ctcccttcaa gtgagtggca gttccattta gcggtttatg
     5281 aggctcgccc tgaattaaat gcggtagttc acaaccacgc taaaaactgt gccgcagtgt
     5341 ctattttagg tgaacctatc cctgccattc actatatgat cgcctgcacc ggcactaatc
     5401 acattccttg tgtaccttat gccccgttcg gcactcacca attagccgac tatgtacgtg
     5461 aaggcattaa acacagcaaa gcaattttac tcgctcacca cggcttaatc accgcagata
     5521 aaaccctcga aaaagccctc ggcatcgccc acgaggtgga agtgatcgca gaatggtatt
     5581 tgaaactgct cgctacagga aaacctatcc caacgctaaa caaagaacaa atggatatcg
     5641 tactcggaaa atttaagtcc tatggttctt gggtggaaag cgaacaatag cctgccataa
     5701 ataacataca agcggtgata tttcttaaaa tttttacaaa cggagtgcag tatgcaaaat
     5761 gaagtaatac tcacgatgaa aaacatcagt aaatcctttg cgggggtcaa tgcgttaaaa
     5821 ggtgttgagc ttacgctgcg taagggcgaa gttcacgcat taatgggcga aaacggtgca
     5881 ggtaaatcca ccttgatgaa atgcttgctg ggcgtatatc aagccgatga aggtgaaatc
     5941 atatataaag gtcagcctgt tcgctttgag tcggtattag aggcacaaaa agcgggcatt
     6001 agtatgattt tccaagaact gaacctcatt ccccatttaa cggtggcaga gaatattttc
     6061 ttcgcccgtg agccactcaa acacggttta gtggataaag acaaaatggt ggaagaatcg
     6121 gctaaattgc tgaatttctt tgagattgat gttgatccaa acgatgaagt gagaatgttg
     6181 tcggtagcca aacaacagat ggtagaaatt gccaaagccc tttcatttga tgtggaagta
     6241 ctgattatgg acgagccgac ctccgctctt accgaaaaag agatcgataa attatttgaa
     6301 ttggtcgata gactgcgtgc caaaggggtg agcattgtct atatttctca ccgaatggaa
     6361 gaattaaaac gtatctgtaa ccatattacg attttccgtg atggcaccta tgtatctaac
     6421 cataaattca atgaaatcac gatggatgag atcattacca aaatggttgg gcgttcattg
     6481 gataaccatt tcccaccaaa aaccgctgtg gtgacaaacg acattatcct ctcgattatg
     6541 aatgctgaac gtaagggagt gtttgaacca ttgaactttg acttacgcaa aggagaaatt
     6601 ctgggcatta ccggcttggt tggagcaaaa cgtactgaac tggctcgagc catcttcgga
     6661 gcagacccac ttgatagcgg tgagattttt gtgcacgaga acaaagtcag cattaaagat
     6721 ccgagtgatg caattaaagc cggtatagcc tatttgtcgg aagaccgtaa gctcaatggt
     6781 gttgcagtaa gaatgtcgat tcgtgagaat atcacgatgg catctatgga taaagttgcc
     6841 aatcaaatag gtgtgatttc ttacgatgaa gaagaaaaag cctcaaaaac tttcatcgac
     6901 aaaatggaaa tcaaaacccc aacgattgag cagaaagttc aaaacttaag tggtggtaac
     6961 caacaaaaag tggtaattgg taaatggtta ttccgtgaag caaaagtgat gatttttgat
     7021 gagccgactc ggggaattga tgtcggagca aaatatgcta tctatcaact gctcgatgag
     7081 cttgctgcca atggcgtggg tgttatcgtg atctcttccg aattgcctga agtcttggga
     7141 gtttccgacc gcatcattgt aatgcgtgaa ggtagaatga caggtatgct cgagactaaa
     7201 aaaaccaacc aagaagaaat tatgcattat gcaactggtg ttaaaaatat gtttgctcgt
     7261 gaatatgggg tggaaaaatg aatgaaaaac aaaaagaaat gttaagaaaa atggctgcat
     7321 tggctggctt agtactatta atcatctttt tcagcgtaac caatgaatac ttctttacct
     7381 ccaataacat tatgacagta gggctacaaa cctctaccat tgcgttaatt ggtattgggg
     7441 caacctgtgt gatcttaacc ggtggaattg atttaagtac cggttcagta gttgcactct
     7501 ccggtgtggc agctgcaatg attgtaaatg ccggtgtacc cgtaccacta ggaatgattt
     7561 tagggatttt ggtcggcggt atttgtggct taatcaacgg cattttagtc accagaatga
     7621 aattgcctcc gtttattgct accctcggta tgatgatggt tgcccgtggt ttagcattgt
     7681 atgttaccaa tgccgcacca gtttccggaa tgcctgaatc ctttgccaat ctcggtaacg
     7741 gagcattatt taaaattgtg gaagaagggg caaacggctt gccgaaagtg gtatttgcag
     7801 gtattcctta ccctgtgatt attatgatct ttattaccgt actattcact ttcgctctct
     7861 ctaaactgaa agtcggacgc tatctttacg caattggttc taacgaagaa gccgcacgct
     7921 tatccggcat taaaaccaat attgtcaaaa tctatgccta tgttgccagt ggcttactct
     7981 ctggtttaac tggcgttatc cttgcctccc gcctcgtcac cgcccagcca aacggtgggg
     8041 tctcttacga gctggatgcg attgccagtg ctgtggtggg cggcacatcg cttatgggcg
     8101 gtgtgggtac tatccccggc acactcatcg ggtcattcat tatcggggta ctccgtaatg
     8161 gtttgaatat gaatggcgtt tcttcatttg ttcaaatgat tgtcatcggg cttgtgatta
     8221 tcgtggcggt ctctctcgac cagctccgcc aatccaaaaa agccggtaaa aaataatcca
     8281 ctcttaaagg agaattaaaa tgaaaaaatc agcattattt actgcgaccc tctttggttt
     8341 atcgacctta tttgcaggca gtgcaatggc aaaatctgat gaaatcgctg tgattgtgaa
     8401 atctgccaac tctaccttct ggcaaaacgt gcgtaaaggg gctgaaactg ccggcaaaga
     8461 tattggagga caatacaaag tcaccttcca agggccggaa gccgaaacag cgattgacgc
     8521 ccaagtgaat atggtggaca acgccgtaaa ccgtggcgtg gcaggcatcg tactggctgc
     8581 ctcagacccg gtagcattag tgccggcggt gaaaaaagct tatgagagtg gcattccggt
     8641 ggtgcttatc gattccggca tcaacagtga cggcaaatac taccaatctt tccttgccac
     8701 cgacaaccgt gcagcaggta aattagcagc ggaaaaactg cttgccaaag tcaaaggcgg
     8761 caaagtggca gtgatgagct ttacccaagg ggcagcatca gcgattgagc gtaccggtgg
     8821 ttttattgac gaaatcaaag caaaagccga ttacaaactg gtcggcccgt actactccga
     8881 ttcggatatg accaaagcgt taaaccaaac cgaagacgta ttaggttcta accctgatct
     8941 tgcagcgatc ttcggggcga acgaaccgac tgcggtagga atggcacgag cggtgaaaca
     9001 aaaaggcttt gccggcaaaa ttgtagcggt gggctttgat ggaaactccg acctacaaaa
     9061 cttcgtgcgt gacggaacac tagacggcat cgtggtgcaa tcttcctacc aaatgggcta
     9121 taaaggcgtg gacaccatcg gcaagctgat taaaggcgag aaagtcgaaa aagtgattga
     9181 taccggggtg gtttatgtca cgaaagaaaa cattgatagc gaagaagcga aaggtgttct
     9241 ttactaacat tcaagttaaa cggggtgggg ttgcccaccc tttcagccga aaggaagcca
     9301 atatgtcaaa tacccttacc gataaagcct tagtggtgat tgccgcactt ggcggtcgag
     9361 aaaatattgt cgatgtttac gcctgtatta cccgcctacg tgtggaggtg aaaaataccg
     9421 acattgtgga atgggataaa attaaatctg tcggtgcgtt aggcgttgtg acctcaggct
     9481 cagttattca atctattttt ggtagcgatg ccgaagccta ccgtgatgaa atcaaacgta
     9541 ttttgaatat ttagcctaag cacactcaca acaaaagaat cgcaacaagc ggtgaaattt
     9601 ttatcaaaat ttgcaaaaaa atgacgtaat ttgaccgctt gcaagttaat cgaattgttt
     9661 aacttttaag aaggaaaata gtatggcaaa tcgtcttatc ctcaatgaaa ccagttacca
     9721 cggcaaagga gcaatccaaa atattgttca cgaagtgacc agccggagtt tcaaaaaagc
     9781 ccttgtggta acagacaaag acctgatcaa atttaacgtt gcccaaaaag tcaccgcctt
     9841 gcttgatgcc gcaggcttgc cttatgagat tttcgatgag gtcaaagcca acccggcagt
     9901 cgatgtgatc aaagcggggg tcgcaaaatt caaagcctcc agtgccgatt atttaattgc
     9961 gattggtggc ggctcaccga ttgatagtgc caaagcgatt ggtattatca tcaataaccc
    10021 tgagtttagc gatgtacttt cattggaagg tgtcgcccca acccaccaaa aatgtgtgcc
    10081 gattatcgcc attccaacta ccgcaggtac agccgcagaa gtcacgatta actatgtcat
    10141 taccgatgaa gcgaaaaaac gcaaatttgt gtgcgtggac gttcacgatg tgccggcagt
    10201 ggcgttagtc gatccagata tgatggaatc tatgccgaaa agcttggctg ccgcaaccgg
    10261 tatggacgct ctcacccacg ccattgaagg ctacattacc aaagcggcgt gggaattaac
    10321 cgatgctcta caccttaaag cgattgagct gatcggccgt tcattgcgtg gtgcagtgga
    10381 gaaaaatccg aaagatgtcg aggcaatggc attgggacaa tatgtggctg gaatgggctt
    10441 ctccaacgtt gggcttggcg cggttcacag tatggcacac ccgctttctg cctattacga
    10501 caccccacac ggcattgcca acgcagtatt actgccgtat gtgatggaat ttaataaaaa
    10561 ttacaccggc gaaaaatacc gtgaaattgc ccgagcaatg ggggtaaaag gcgtagatga
    10621 aatgagccaa gaagagtacc gcaacgccgc cattgctgcc gtacaacagc tttcacaaga
    10681 tgtcggcatt ccgccaaaac tgcatcaaat cggcgttaaa gaagccgatt tagcggcatt
    10741 atcggtcgat gcctttaatg atgtctgcac cggtggcaac ccgagagatt gtacggctga
    10801 ggagctgttg gaggtatatc ggatcgcata ctaagttcta tctttgttaa atagacaagc
    10861 ggtgattttt taataaaaat ttgcaatttc tgt
//
LOCUS       DANLUQ010000077        10892 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.077, whole genome shotgun sequence.
ACCESSION   DANLUQ010000077 DANLUQ010000000
VERSION     DANLUQ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10892
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.077"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(76..381)
                     /locus_tag="PXB27_001529"
     CDS             complement(76..381)
                     /locus_tag="PXB27_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HDL5346304.1"
                     /translation="MRKPAHPGQILLDGFIEPNNIKIKELADHLGFSRETLSRVLHGK
                     TPMTANLALSLEEAGISTAKLWLNLQTKYDLWELKQQRLNPVMPFHFGGNYGETAHA"
     gene            complement(390..665)
                     /locus_tag="PXB27_001530"
     CDS             complement(390..665)
                     /locus_tag="PXB27_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011039853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5346305.1"
                     /translation="MIKSFKHKGLKQYFEKGITKGIQLNHQRKIDGILDLIDSAESID
                     EFMPFYQCHELKGNRKGIYSMTVNGNWRITFEFVNGDAYILNYEDYH"
     gene            957..1187
                     /locus_tag="PXB27_001531"
     CDS             957..1187
                     /locus_tag="PXB27_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346306.1"
                     /translation="MNAQATLNNHKDYILCGRKEKRTSDFINVFEVFENEATQEFVIE
                     RAMFRNGKLIDWNQSDKMNAEQAQQLWQAYIH"
     gene            1569..1805
                     /locus_tag="PXB27_001532"
     CDS             1569..1805
                     /locus_tag="PXB27_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346307.1"
                     /translation="MSNQEFVKLGSVEPSTIDELQKEDIRQAILKSVSITPYYVGLEI
                     AVCQAISAINKFSDGCITEELLDTKNGDKYRKNN"
     gene            2112..2187
                     /locus_tag="PXB27_001533"
     tRNA            2112..2187
                     /locus_tag="PXB27_001533"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2145..2147,aa:Arg,seq:tct)
     gene            2374..2880
                     /locus_tag="PXB27_001534"
     CDS             2374..2880
                     /locus_tag="PXB27_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346308.1"
                     /translation="MKTKYKIIIERAKQQGLTHIELTQKHCLYLATSVGKLCYGVANN
                     AEQHIKLAAGEIFTLLTIINHQIQFDEDLELHTWKASGTTPPEQRAIRMLHHTSQFAW
                     FAPWLVDSNEKEQNQNTIRINLFNKCSGILIELNGILKEYELTLDECLDEFLKNKHCL
                     NEFSIHKE"
     gene            2884..3243
                     /locus_tag="PXB27_001535"
     CDS             2884..3243
                     /locus_tag="PXB27_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346309.1"
                     /translation="MTTFSIYTSVWHDSSTKGFRHNIKHKRHDCWRADIRIKQKINDV
                     WVQISRIRKRFKCPEQAKSFVEQWKQIDSPAFMQHQINRQALREELSNLEQRKQKQDG
                     ILEVIEYRISQIKELLK"
     gene            3240..3719
                     /locus_tag="PXB27_001536"
     CDS             3240..3719
                     /locus_tag="PXB27_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346310.1"
                     /translation="MKRNKVESYTIRLLKNNHTKRYFGEVILNGIKEISTAEMMSEIN
                     AIKAVNKRIEALNKTKAVNLPNYPEIRGHQPIMYNYNFEQIEEDPEQVKPKPSKPKRK
                     PFIPYGLNGYFVDSNGNIRLHLDRRASAETITLETDFFNVLAEMVKRTQEANNAKHA"
     gene            3853..4065
                     /locus_tag="PXB27_001537"
     CDS             3853..4065
                     /locus_tag="PXB27_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346311.1"
                     /translation="MIGYEDYTDASLQPEEDPSEEAFILAEENIIELLENNGFETIAT
                     ILAGSDGWRNAINAYLAEQNKHRNEF"
     gene            4078..5028
                     /locus_tag="PXB27_001538"
     CDS             4078..5028
                     /locus_tag="PXB27_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346312.1"
                     /translation="MTTANQQFELILKTESRVVSTNLQTFEEQANQYLATLTTTFETD
                     DDFAKAKEEVKELESIEKKIRDAIKQTQTGEIAELVLTAEQIAERFRQERLTRDKLVK
                     SKETEIKKQIADKAIEEITDTRNKLVKVSDISLALEITMPKHGIANRIAEAQKNKRTI
                     DSLTKAVNAEKALIISEMSVEIGRLTERLEQLAAKSAYLFPDAVKLIATQDDLAPIIQ
                     QRLAEEAEREAAIKAKAEADKVQAEAKAITDKMERKQAVENPQNVAKMNIEEPAQTDG
                     PLGDFIITIRLNQTIQTNAVSIARELKAKFGDSVSLNKAK"
     gene            5069..5863
                     /gene="bet"
                     /locus_tag="PXB27_001539"
     CDS             5069..5863
                     /gene="bet"
                     /locus_tag="PXB27_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HDL5346313.1"
                     /translation="MTTALQTLTNKLAERFEMGDGSGLVETLKSSAFAGATVSDAQMI
                     ALLVIANQYQLNPWTKEIYAFPGGNGGLTPIVGVDGWVRIINREPQYDGMEFHFTDDY
                     SACTCTIYRKDRSKPIVVTEFMGECKKSSPAWNSHPKRMLRHKAMIQCARLAFGFTGI
                     YDQDEAERIAENEKPPKNITPQNNVVETTAVELISEEQLSQIRQLMQVTGTEEAKILA
                     YIGVQALNQIPKSQAEAVIKKLNLTLDKQNAEKADNGESVGEEIPL"
     gene            5860..6495
                     /locus_tag="PXB27_001540"
     CDS             5860..6495
                     /locus_tag="PXB27_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HDL5346314.1"
                     /translation="MTGDLIILDCEQGSEEWLNARLGIPTATGIENIVTPSGKKSSAW
                     NSYLAELVAESIEGAKDGFKSSDMARGNELEPLARMAYEFETDTEVVQVGGVYLNAEK
                     QLMVSPDGLIPNLKKGLEIKCPKMKTHIKYLLEGGVPNEYLIQVQSALWVTGYETWDF
                     VSYCPEYQKQPLYIYTAERDPVLMKAFDQYIPQFIKSLYALKGVTAPKGAF"
     gene            6508..6684
                     /locus_tag="PXB27_001541"
     CDS             6508..6684
                     /locus_tag="PXB27_001541"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346315.1"
                     /translation="MTTQKKYELLQNDTINHNGRTLYRIKAICHFRRTAEYSARATHA
                     TAAECYGGIACTST"
     gene            6705..7157
                     /locus_tag="PXB27_001542"
     CDS             6705..7157
                     /locus_tag="PXB27_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDL5346316.1"
                     /translation="MKPFDLNKALAGEPVKLRNNDKAFVKYLISDDYIRDNKDHQVQG
                     YTVDEENVFLSEVSWAVSGSHFNDGTIAQYDIVGMWEEPRPAVTLTLPCPLKEPQENM
                     WFIDNDFTIVKSMFANAPFVKKFLPQGRCFASEEDAQEWLDAMRNSRR"
     gene            7235..7762
                     /locus_tag="PXB27_001543"
     CDS             7235..7762
                     /locus_tag="PXB27_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HDL5346317.1"
                     /translation="MNEEKYFSVDVSDETHVIRLHETLEQAKQSCLNGATEAYEFAGD
                     MDDHESYESYEAYDLPYAVYGVVLGRAKSDIRPLTDEERASELFGEAEQVIEPPTLLE
                     NNGWISIEDKLPPIETDVLGLCDISGMQLILIVSRELADNEWYFLSVNQYGLDDDVIA
                     VTHWQPLPEPPKEEK"
     gene            7759..7962
                     /locus_tag="PXB27_001544"
     CDS             7759..7962
                     /locus_tag="PXB27_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346318.1"
                     /translation="MTDTERLDFIAQRKLDVRYSDLVDEFIIASYTKPHEQYFHWARN
                     ADLRTAIDEAMKEMRGLNNATTN"
     gene            7946..8410
                     /locus_tag="PXB27_001545"
     CDS             7946..8410
                     /locus_tag="PXB27_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDL5346319.1"
                     /translation="MQQLIKNIEQWAEDRNLINGSTPQKQMLKLMEEFGELCGGIARN
                     NPEMIKDAIGDLIVVCIIKAKQEKQPIYPVQFENHRLNGYRFDISVAVHCLYDIERIK
                     DRGKYSRFFGILHHLALINRFTLNDCLAHAYDQIKDRKGEMQAGIWVKEDDL"
     gene            8504..8674
                     /locus_tag="PXB27_001546"
                     /pseudo
     CDS             8504..8674
                     /locus_tag="PXB27_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253375.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8910..9401
                     /locus_tag="PXB27_001547"
     CDS             8910..9401
                     /locus_tag="PXB27_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL5346320.1"
                     /translation="MQTKPILDACCGSRMFHFDKQNPLVLFADNRQIETTMKDRDKIR
                     HLEIKPDVLHDFTNMPYENESFWHIVFDPPHLVQGGENSWLVKKYGKLPCNWREVLKK
                     GFDECMRVLKPNGTLIFKWNETQIPVSEILKVIDCQPIYGHKSGRLNQTHWLAFVKIE
                     QSL"
     gene            9428..9685
                     /locus_tag="PXB27_001548"
     CDS             9428..9685
                     /locus_tag="PXB27_001548"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346321.1"
                     /translation="MEQIKLSEKAERALILSQLNENSAMIIDLDDVALILNCGYDYVQ
                     SKISKKPGFPEPVQGINGKSVLFKSGDVIRWVNRQARTRKN"
     gene            complement(9682..10671)
                     /locus_tag="PXB27_001549"
     CDS             complement(9682..10671)
                     /locus_tag="PXB27_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL5346322.1"
                     /translation="MASIRKRGSKYQVEISKKGVRKSATFRTKAEAQAWAYEEERKIE
                     IISKGGVVDILFAEALERYQNEVSINKRTWRREIGRINNILKEPIANRYVQDIKRDDI
                     EDWIEARLKRGLKGSSVERDLSILSNVFTYCLKRWGYISENPMIGVIHPNKVPPRKQR
                     YSQEDIDKILEVSKYREDIKRSNARLAAAMLFAIETAMRAGEISGLTWDNVNFEKRIA
                     HLPMTKNGSSRDVPLSSKAIEILKRLETIKKDNSVFQLPQGNGLSARFFELKEKAGLS
                     YLNFHDTRREALSRLAKKVDVMTLAKISGHKDVKILLNTYYAPNMEEVAHLLD"
BASE COUNT         3691 a         2217 c         2147 g         2837 t
ORIGIN      
        1 atattccttg aataaaccgc cctcgtggcg gttttctttt cctaagaaca ataaaatcct
       61 ccgaagaggg cattattaag cgtgagcagt ttctccataa tttccaccaa aatgaaaagg
      121 cataacagga tttaaccgct gctgctttaa ctcccataaa tcatatttag tttgtaagtt
      181 tagccataac ttagccgtac taattcctgc ctcttctaaa cttaacgcta aatttgcagt
      241 cattggtgtt ttgccgtgta atactcttga taaagtttca cgagaaaacc caagatgatc
      301 tgctaattct ttaattttga tgttatttgg ttcaataaat ccatctaata aaatttgccc
      361 cggatgtgct ggtttacgca taactccccc taatgataat cttcataatt caaaatataa
      421 gcatcgccat ttacaaattc aaatgtaatc cgccagttgc cattaaccgt catagaataa
      481 atgccttttc gatttccttt taactcgtgg cattggtaaa aaggcataaa ttcatcaata
      541 ctttctgctg aatcaatcaa atctaaaata ccatcgattt tacgttgatg attaagctga
      601 ataccttttg ttattccttt ttcaaaatat tgttttaagc ctttatgctt gaagctctta
      661 atcataataa cacctagcaa acacaatgtg atacaaatat atcacgcaca ataaaaaaga
      721 caatcttttt taagtgcttt ttctcctaat tgtctttctc ttcttatgtt aagtgtttaa
      781 aaaacaagca atcaaacaac ctttctaaaa ataatttctc tttaaaatca attatttaat
      841 cacgaattga aatttgtttg gctaaaataa ttacaaattg aattgactta atgatttcaa
      901 tttgtgataa taaacccatc aaaacaaaca acgccacaga caaagaggaa accaaaatga
      961 acgcacaagc aacgctaaat aaccacaaag actacatttt atgcggacgc aaagaaaaac
     1021 gcaccagtga tttcatcaat gtgtttgagg tctttgaaaa tgaagccacg caagagtttg
     1081 tgattgaaag agcaatgttc agaaatggca agttaatcga ctggaaccaa agcgacaaaa
     1141 tgaacgccga gcaagctcaa caactttggc aagcctacat tcactaagaa tttttatcaa
     1201 agcccttcac ggagggcttg aataaaggtt ctaaacctcg ccgaaaggca ttgttcttta
     1261 aaaattaaac caaaaacaca tcacgacaag cggattactt agcaatgtaa tgacattgga
     1321 gagacagaac caatctttgc ataaagcggt aaaacgtatg cggtaaatga acttataggc
     1381 aagttgtgaa actctctaag ctactcggac tgaaagaata gtacggtagt tgtggcgaat
     1441 ttactggtga tgtgcgaact agcggaaagg ttaagcactg gcagtgaatc gggaagcaca
     1501 aacgctggtt caattccaaa gcatatttga gaaagtgtgc tttggaatat taataacagg
     1561 agaacaaaat gagtaatcag gaatttgtta aattaggcag cgttgaacct tcaacgattg
     1621 atgaattaca gaaagaagat attaggcagg caatattaaa atccgtctct ataacgccat
     1681 attatgtggg gctggaaata gcggtttgcc aagcaatatc agccatcaat aaatttagtg
     1741 atggctgtat tacagaagag cttttagata cgaaaaatgg tgataaatac cgaaaaaata
     1801 actaatcaat aacaccaaag tgatttcatt ttttcggcat tagccctaga tgttgcccaa
     1861 gtattatatt cctcgaagtt gtggcaaaca agaggacttg gcccggtggc tcacaagtat
     1921 aaaaaagagc caccatctct aacagctctc aacagagagt gagacaaata accccgtttt
     1981 agttttaatg ttttctaaag tcggcgttag gcagcgaaac gaatgtacta gagagttgtt
     2041 agagatggtt agtccatcac attccttaga ttgaagtaag ccctgccgtt ggggttaagc
     2101 aacggtactt tgaccgcata gttcagtgga tagagcagct gccttctaag cagtgggtcg
     2161 agagttcgaa tctctctgcg gtcgccatcc aaaaccgctt tacgcacatc atcgatgacg
     2221 agcctacgaa gtggaaacct accgtgtaga gcggttttga atggctaagt tgtgtaactg
     2281 ctacaactta aaaagagcgg ttgataataa acataatgac tccgtttccc cgcctcgtgc
     2341 ggggcttttt ttatccacat aaaaggattc aaaatgaaaa ctaaatacaa aattattatt
     2401 gagcgagcaa aacaacaggg tttgacccat attgagctta ctcaaaaaca ttgtctgtat
     2461 ctcgctacat cagtagggaa actgtgctac ggggtagcta ataatgcaga acaacatatt
     2521 aaacttgctg ctggagagat ttttacacta ctaacgatca ttaaccatca aatccagttc
     2581 gatgaagact tagagcttca cacgtggaaa gctagtggaa caaccccacc cgagcaaaga
     2641 gcgatacgta tgttacatca tacctcgcaa tttgcttggt ttgctccttg gttagtggac
     2701 agcaatgaaa aagaacaaaa ccaaaataca atcaggatta atttatttaa caaatgctct
     2761 gggattctga ttgaactaaa tggcattctt aaagaatacg agctaactct tgatgagtgc
     2821 ttagatgaat tcttgaaaaa taagcattgt ttgaatgaat ttagtattca caaggagtaa
     2881 aaaatgacga cgtttagtat ttataccagt gtatggcacg acagctccac gaaaggcttt
     2941 cgccacaaca ttaagcataa acgccacgat tgctggagag cggatatccg cataaagcaa
     3001 aaaatcaatg atgtgtgggt gcagatttca aggatacgca aacgcttcaa gtgccctgaa
     3061 caggcaaaat cctttgtaga acaatggaaa caaattgatt cacctgcatt tatgcaacac
     3121 caaatcaacc gccaagcatt aagagaagaa ttatcaaacc ttgagcaacg caaacagaag
     3181 caagacggaa tacttgaggt gattgagtat cgtattagcc aaatcaagga gttgctgaaa
     3241 tgaaacgcaa taaagtagaa agctatacta tccgcttatt aaaaaataat catacaaaac
     3301 gctacttcgg agaagttata ttaaacggca taaaagaaat cagcacggca gagatgatgt
     3361 ctgaaattaa cgctattaag gcagtaaata agcgcattga agctctgaat aaaaccaaag
     3421 ccgttaattt gcctaattat cctgaaatcc ggggtcatca gcctattatg tacaattaca
     3481 attttgaaca aattgaggaa gatcctgaac aggttaagcc taaaccatca aaaccaaaac
     3541 gcaagccatt cataccttat gggctaaacg gctactttgt ggatagcaac ggtaatattc
     3601 gcttacatct agacagacga gcaagtgccg aaacaattac ccttgaaact gatttcttca
     3661 acgttcttgc cgaaatggta aaacgtacac aagaggcaaa caatgctaaa catgcttaga
     3721 caatggatta aatacggctt atacgccctg ctttttatgg ttgccatcac cctcctaatg
     3781 caatgaagag cttatccctg aaagcacctg caagcctgaa acttgcgaat ttaaagtaac
     3841 ggagaaagtg tgatgatagg atacgaagac tacacagatg cttcacttca accagaagaa
     3901 gaccctagcg aagaggcttt catcttggct gaagaaaaca tcatcgagct attagaaaac
     3961 aacggctttg aaaccattgc caccatccta gccggttctg atggctggcg taacgcaatc
     4021 aacgcctacc tagccgaaca gaacaaacac agaaatgaat tttagaggaa taagaaaatg
     4081 acaactgcaa atcaacaatt tgaacttatt ttaaaaaccg aaagccgagt ggtttctacc
     4141 aacttgcaaa cttttgaaga gcaagcaaat caataccttg ccacgctcac caccaccttt
     4201 gaaaccgatg atgattttgc aaaagcaaaa gaagaagtga aggagcttga aagcattgag
     4261 aaaaaaattc gtgacgcaat taaacaaacg caaactggcg agattgcgga attggtatta
     4321 acagcagaac aaatcgctga acgtttccgc caagagcgat taacccgaga caagctcgtt
     4381 aagagcaaag aaacggaaat caaaaaacag attgccgata aagccattga agaaatcact
     4441 gataccagaa acaagctggt taaagtaagt gatatttccc ttgccttaga aatcacaatg
     4501 ccaaaacacg gcattgctaa tcgtattgcc gaggcacaga aaaataaacg cacgattgac
     4561 agcttaacca aagccgtgaa cgccgaaaaa gcgctgatta tcagcgaaat gtcggtagaa
     4621 atcgggcgct tgactgagcg attagaacag cttgctgcaa aatcagctta tctttttcct
     4681 gatgcagtga aattgatcgc aacccaagac gacctcgccc caattattca acaacgcctt
     4741 gccgaagaag ccgagcgtga ggcggcgatt aaagccaaag cagaagccga caaagtacaa
     4801 gccgaagcca aagcgattac tgataaaatg gagcgcaaac aagcggtcga aaatccgcaa
     4861 aatgtggcaa aaatgaacat cgaagaaccg gcacaaaccg atgggccatt aggcgatttt
     4921 atcattacta ttcgcttaaa ccaaacaatc caaaccaacg cagtcagtat tgctcgtgag
     4981 ctgaaagcga aatttgggga tagcgtttcg ctcaataagg ctaaatgatt tgataccgca
     5041 caaattcaac ctaaataagg atcatattat gacaactgca ttacaaacac taaccaacaa
     5101 acttgccgag cgttttgaaa tgggagatgg ctcaggatta gttgagacac ttaaatcgtc
     5161 tgctttcgca ggggcaaccg taagcgatgc tcaaatgatt gcactattgg ttattgctaa
     5221 ccaataccaa ttaaaccctt ggacaaaaga aatttatgca ttccccggtg ggaacggtgg
     5281 attaacgcct attgtcggcg ttgatggttg ggttcgcatt attaaccgtg agcctcaata
     5341 tgatggaatg gaatttcatt ttaccgatga ctattccgcc tgtacttgta ctatctaccg
     5401 taaagataga agtaaaccga ttgtggtgac ggaatttatg ggggaatgta aaaagagctc
     5461 tccagcttgg aactctcacc caaaaagaat gttacgccat aaagcaatga tccaatgtgc
     5521 aagattagcc tttggcttta cgggtattta cgaccaagac gaagcagagc gtattgcgga
     5581 aaatgaaaaa ccacctaaaa acatcacacc acaaaataac gtggtagaaa ccactgccgt
     5641 tgaactcatt tcggaagaac aactgtcgca aatccggcag ttaatgcaag taaccggtac
     5701 ggaggaggcg aaaattctcg cctatattgg cgttcaagca ctgaaccaaa tccctaaatc
     5761 gcaggcagag gcagtgatta aaaagctcaa tcttacgcta gacaagcaaa acgctgaaaa
     5821 ggcggataat ggcgaaagtg tcggtgagga aatcccacta tgacaggcga tttaatcatt
     5881 ttagattgcg aacaaggatc agaagaatgg ctcaatgcca gattgggcat tcccaccgcc
     5941 acaggcattg aaaatatcgt tacgccaagc ggtaaaaaat cgtccgcttg gaatagctat
     6001 cttgccgaac tggttgccga aagcattgag ggagcaaaag acggttttaa gtcgtctgat
     6061 atggcacgag gcaacgagct tgaaccgctt gcacgaatgg cttacgagtt tgaaaccgac
     6121 accgaagtcg tgcaagtcgg cggtgtgtac cttaacgccg aaaaacagtt aatggtaagt
     6181 cctgacggct taattcccaa tctaaaaaaa gggttggaaa tcaaatgccc gaaaatgaaa
     6241 acccacatca aatacctgct agaaggcggt gtgccgaatg aatatcttat tcaagtgcaa
     6301 tctgcccttt gggtaacagg ctacgaaacg tgggactttg tcagctactg ccccgaatac
     6361 caaaaacaac cgctttacat ctacacagcc gaacgtgatc cggtattgat gaaagccttt
     6421 gatcaatata tcccgcaatt tatcaaatcc ctttatgcat tgaagggggt aaccgccccg
     6481 aaaggggctt tttaataggt agccaatatg acaacccaaa agaaatacga actactccaa
     6541 aacgacacaa tcaaccacaa cggcagaaca ctctaccgca tcaaagccat atgccacttt
     6601 aggcggacgg ctgaatattc cgcaagagcc actcacgcca cagccgctga atgctatggt
     6661 ggaattgcct gtacatcaac ataaccacaa acacggagaa taaaatgaaa ccatttgatt
     6721 taaataaagc attggcaggt gagccagtaa aactaagaaa taatgataag gcatttgtaa
     6781 aatacctaat tagtgatgac tatattagag ataataaaga ccaccaagta caagggtata
     6841 cagttgatga agaaaatgta tttttgtcag aagtatcgtg ggctgttagc ggatctcatt
     6901 tcaatgatgg aacaatagct caatatgata ttgttggtat gtgggaagaa ccaagaccag
     6961 cagtaacatt gacgttacct tgcccattaa aagaacctca agaaaatatg tggtttattg
     7021 acaatgattt taccatagta aaatctatgt tcgcaaatgc cccctttgta aaaaaattct
     7081 tgccacaagg gcgatgtttc gcttcagaag aagacgctca agaatggtta gatgcaatgc
     7141 gaaatagtcg taggtaaatc ggcagcacta acagctgaat gctgctgttg aatttcttgt
     7201 atatccacat aaacacggaa cgcggagaaa gaaaatgaat gaagaaaaat atttttcggt
     7261 cgatgtctcg gatgagacac atgtaataag gttgcacgaa acattagagc aagcaaaaca
     7321 aagctgttta aatggtgcta ctgaagccta tgagtttgcg ggtgacatgg atgatcacga
     7381 aagctatgaa agctatgaag cctatgattt accctatgca gtttatggtg ttgttttagg
     7441 tagagcaaaa tcagatattc gtccactaac agacgaagaa agagcatcgg agttatttgg
     7501 ggaagctgaa caagttattg aaccaccaac acttttagaa aacaacggct ggatttccat
     7561 tgaggacaaa ttaccaccta ttgaaacgga tgtgctaggg ttatgcgata tttcaggaat
     7621 gcagttaatt ttaatagttt caagagaact tgccgataac gagtggtatt ttcttagtgt
     7681 aaatcaatat gggcttgatg acgatgtgat tgcagtaacc cactggcaac cacttcccga
     7741 accaccaaag gaggaaaaat gaccgacacc gaacgcttag actttatcgc ccaacgcaaa
     7801 ttagatgtcc gatattctga tttggtggac gaatttatta ttgctagcta taccaaaccg
     7861 cacgagcagt attttcattg ggcaagaaat gccgatttac gcacggcgat tgatgaagca
     7921 atgaaagaaa tgagaggact aaacaatgca acaactaatt aaaaacatcg aacaatgggc
     7981 ggaagaccgc aatttaatta acggctcaac cccacaaaag caaatgctca aactgatgga
     8041 agagtttggc gaactctgcg gaggcattgc ccgcaataat cctgaaatga ttaaagatgc
     8101 gattggggat ttgattgttg tctgtattat taaagcaaaa caagaaaaac aacctattta
     8161 ccctgtacaa tttgaaaatc atagattaaa tggttacaga tttgatattt cggtcgctgt
     8221 tcattgtcta tatgatattg aacgcattaa agacagaggt aaatatagcc gtttttttgg
     8281 catattacac catttagcct taataaatag atttacgctt aatgactgcc tcgctcacgc
     8341 ctacgaccaa atcaaagatc gcaagggcga aatgcaagct gggatttggg ttaaagaaga
     8401 cgatttatag tacctaagct ataaaaaccg aaacatcttg cagaaaagca ttccgccact
     8461 ctataagagt ttcggtttct tcttcgttgg cgttgattat gtttacacgc acgctaccga
     8521 gtatcaaacc acgcttggcg taatgaagcg gatacttgaa ccgatgttag ccgacttcga
     8581 ggtcgatccg tttgaagagg ctcactacca caaagcccta actaccttaa ggcaatacca
     8641 gccgaaaggc ttaggcggct tagtccgaat ttaatttgca aaaaaatcac acaatccgac
     8701 cgcttgtgaa acaacaagcg gcggatttcc atacccaaat tttgaggaca agccgatgaa
     8761 atacgcaaaa atcattctat ttttaaccgc ctttgcagtt gctgctgacc atctagagtt
     8821 acgcaacgac tgcgatggca aaatttgtat ggctcaacgc taattaaccc aaccgcttgt
     8881 tccgcaagcg gttatttttt ggaggaaaaa tgcaaacaaa accgatatta gatgcgtgtt
     8941 gtggttcccg tatgtttcat tttgacaagc aaaatccact cgtcctgttt gctgataatc
     9001 gacaaataga aaccacaatg aaagaccgag acaaaatccg ccatttagaa atcaaacctg
     9061 atgttttaca cgatttcacg aatatgccat acgaaaacga gagtttttgg cacattgttt
     9121 ttgatccgcc acatttagtt caaggtggag aaaatagctg gcttgtaaag aaatacggca
     9181 aattaccttg caactggcga gaagtcttga agaaaggctt tgacgaatgt atgcgagtgc
     9241 taaaaccaaa cggcacgctt attttcaagt ggaatgaaac acaaatcccc gtttcagaaa
     9301 ttttaaaagt tattgattgt cagccgattt atggacacaa aagcggacgc ttaaaccaaa
     9361 cacattggct ggcgtttgtg aaaattgagc aatccctcta aatgaggggt ttatttttgg
     9421 agagaatatg gaacaaatta aactttcaga aaaagcggag agagccttaa ttctttctca
     9481 gctcaacgaa aattcagcaa tgattattga tttagatgat gttgccctga ttttgaattg
     9541 cggatatgac tatgtgcaat ccaagatcag taaaaagccc ggtttcccag agccagtgca
     9601 aggcattaac gggaaatcag ttcttttcaa aagtggcgat gttatccgtt gggtcaaccg
     9661 tcaagcacga acacgcaaaa attaatccag caaatgagcg acttcttcca tatttggggc
     9721 gtagtaagtg ttaagcaaaa tcttcacatc tttatgccct gaaattttgg ctaaggtcat
     9781 cacatcgact ttttttgcca agcgtgaaag agcctctctc ctcgtgtcgt gaaaattcaa
     9841 ataagaaagc ccagcctttt cttttaactc aaagaatcta gcacttaaac cattcccttg
     9901 tggaagctga aaaacggaat tatccttttt gatggtttcc agtcttttca agatctcgat
     9961 tgcctttgat gataacggaa cgtctcttga tgaaccgttt tttgtcatag gcaaatgagc
    10021 tattcttttc tcaaaattca cattatccca cgttaaaccc gaaatctcac cggcacgcat
    10081 tgccgtttca atcgcaaata acatagccgc agccaatcga gcattagagc gtttaatatc
    10141 ttctcggtat ttgctgactt ctaagatttt atcaatatct tcctgggaat aacgctgttt
    10201 tcttggtggc actttgtttg ggtgaataac accgatcatc ggattttcac taatatagcc
    10261 ccagcgtttt aagcagtagg taaatacatt gcttaaaata gacaaatccc tctcaacact
    10321 tgagcctttt aaccctctct tcaatctagc ttcgatccaa tcttcaatat catctcgctt
    10381 aatatcttgt acataacgat tcgcaatagg ctctttcaat atgttattga ttcgacctat
    10441 ctctcgtcgc cacgtcctct tattaatcga tacttcattt tgataccgct ctaacgcttc
    10501 cgcaaataaa atatcaacta ccccaccttt cgagatgatc tctattttac gctcttcttc
    10561 gtaagcccat gcctgtgcct ctgcttttgt gcggaaggta gctgattttc tcaccccttt
    10621 cttcgagatt tcgacttgat atttactgcc ccgcttgcga atgctcgcca tatttttacc
    10681 tgccttttgt cgtaaatttg tcgtaattta taccatacaa taaccgacaa taacagataa
    10741 taaccgattt tatgaaatca tcttaaggca aagaagagag gaaaaactta agataaccta
    10801 ttgaaataac tataataaac gataataacc gacaaaaaac ggaaataaaa aaaagcccct
    10861 aatcaaaaaa gcgaaaaggg gcggaggtct ta
//
LOCUS       DANLUQ010000078        10777 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.078, whole genome shotgun sequence.
ACCESSION   DANLUQ010000078 DANLUQ010000000
VERSION     DANLUQ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10777
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.078"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..392
                     /locus_tag="PXB27_001550"
     CDS             <1..392
                     /locus_tag="PXB27_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDL5346323.1"
                     /translation="YLLMKCISAEKIEVIPVIDQRILTVCDLILTNLAKNESLEAMAE
                     KVFLSPSRLSHLFKQSLGISLVQWRELQRISESKKLLYFSNISISNIAKSLGYEDSLY
                     FSKIFKKHTGLSPSEFRTLERRKDSEM"
     gene            579..1553
                     /locus_tag="PXB27_001551"
     CDS             579..1553
                     /locus_tag="PXB27_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL5346324.1"
                     /translation="MKKPTFKHALFSSILALACYGSAFANDVVNISKIDGMPWFNRMA
                     EGVVQAGKDNNINAYQVGPSNTDAPQQVKLIEDLIAKKVSAISIVPNDANALEPVLKK
                     AKQNGIVVLTNESVGQPSADWDIEIIDNAQFAADYVEEVAKAMGGKGGYVIYVGGLTV
                     PQHNLWADLFVKYQKEHYPDMFEVTSRMPVAENINDARRTTIDLVKAHSDLKAVVSFG
                     SQGPIGAGLAVKEKRLKNKLNVFGMMIPSQAANLIKSGDITMGITYDPANAGYALAAV
                     AAKVLKGEKIEDGLEIPTVGKAKVDSEKKLLQFHNVLKVTKENIDKLY"
     gene            1608..3098
                     /locus_tag="PXB27_001552"
     CDS             1608..3098
                     /locus_tag="PXB27_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL5346325.1"
                     /translation="MAFISMRHISKTFHGIKALNQVNLSLDYGEALCLAGQNGCGKST
                     LIKILSGVYQPDKGAEIQIGATQYTKLTPQESIDKGIQVIYQDLALFPNLTVAENIAI
                     NLHRKFGWVSQDHIHQVALTAIQSINANLDLNAILEDLPIAQQQLVAICRALAQNARL
                     LIMDEPTASLTAKEVKDLLDVVIKLKSKGISIIFVSHKLQEVMSVSDSVLVLKDGNMV
                     GQYPIREMNEKRLGFLMTGLEIDYKRLDLPDFLQRKTVLEVRNLTLPHQYQEINFSLR
                     EGEIVALTGLLGSGRTELCLSLFGITQPQSGDILLNGEKVIFNTNRDAIAKGIAYVSE
                     DRMHTGLIMAESIHHNIISTIFHKITDKLQIIQSSKAYASSQALIDSLKIKVSDSDLP
                     VNTLSGGNAQRVSIAKWLAIDPKIIILDAPTIGVDIANKEGIFQIIKSLAQQGIAVIF
                     VTDEVEETYYNSHRVIVMKKGRIVDEILPAYSSEKSIAEVIYENQQ"
     gene            3082..4041
                     /locus_tag="PXB27_001553"
     CDS             3082..4041
                     /locus_tag="PXB27_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5346326.1"
                     /translation="MKISNNHILSSTLLILILLLTLFTRDFATIENLYDVVNNYAMLM
                     ILACGLFVVLLSGGIDISFPAITIISQYVMVTLIGKYDVGFAGVFVISSTIGILLGFI
                     NALFVNRLSVPSIIITISTLNIFYGGLLYFTKGVWLYEYPEWFTQEIVFFKQITAEGV
                     EFGLSFQALMAILAVILTGLITNKLTIGRKIYALGGNPDATSRIGFNLLFLHLFAYGY
                     MGFMAGLAGIVQSYTVQSVAPDSLLGYELTVLAAVVLGGASLSGGRGTLLGTVMGVML
                     LAIIQNGLNLMNVSSYWQTIITGIIIILSISATALSQRKKQGA"
     gene            4044..4994
                     /locus_tag="PXB27_001554"
     CDS             4044..4994
                     /locus_tag="PXB27_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5346327.1"
                     /translation="MKSKLDPTIFYLLALFAVAVIAFSIFIPDIFWSVSNFQSMASQI
                     PVLGILTLAMALPMLTGGINLSIIAGMNACSLIIAYFITQYTGISWLFIAILLSLCCA
                     LAIGLLNGTLIAIIRVSPILATLGTMTLINGINILVTNGSTIANFPETMLSINASAFL
                     WIPLPILIFLFLAGLIWFFLEKTAMGKAVYFIGNNEKATYYSGINTKKVLIWVYVISS
                     ILCVFAAILMMSKLNSAKASYGDSYLLISILAAVLGGINPDGGKGKLLGIMLALVLLQ
                     VIESGLNMLGISSYITMILWGSLLLFFIFLQKNRLFRLTQ"
     gene            5007..6602
                     /locus_tag="PXB27_001555"
     CDS             5007..6602
                     /locus_tag="PXB27_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HDL5346328.1"
                     /translation="MQNEKQLIEQGNTVIGIELGSTRIKAVLISSDGTILATGGADWE
                     NRLIDGIWTYHQNEIWEKLQAAYMDLSKTVKEKYHTTIRTAKALGISAMMHGYLPFDK
                     HGNQLVAFRTWRNNITLTSSEKLTALFQYPIPQRWSIAHLHQALLNQEPHLPEIDYLT
                     TLSGYVHWQLTDHKVLGIGDASGMFPIDTELKSYNPTMLAQFDRAIADYRMPWKLEAI
                     LPQVRVAGEQAGKLTEKGAKLLDPTDNLQAGIPLCPPEGDAGTGMIATNCIKEKTGNI
                     SAGTSAFAMIVLEKALSKAYSELDIVTTPAGKLVAMAHANNCTSDINAWVNLFGECLS
                     VFGVNICTEELYEMLFLHALQGDADCGGLLSYGFHSGEHNVGLSEGCPVFLHPTNAHF
                     NLANFMRTHLYTAFGAMKLGMDILINQEKVEISRILGHGGIFKTEGVAQKILSSALNI
                     PLATASTASNGGAWGIALLADFLSVSDRYSLEEYLDNCIFNTTKLNLVQPDKTMSEGY
                     ERFMQRYKQGIPVVEKALFLNTD"
     gene            6832..8034
                     /gene="yedE"
                     /locus_tag="PXB27_001556"
                     /pseudo
     CDS             6832..8034
                     /gene="yedE"
                     /locus_tag="PXB27_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005824242.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
     gene            8070..8339
                     /gene="yedF"
                     /locus_tag="PXB27_001557"
     CDS             8070..8339
                     /gene="yedF"
                     /locus_tag="PXB27_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007241376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HDL5346329.1"
                     /translation="MAFTVVQKLDKDPKDITPDYTLDTLGEPCPYPAITMLETMPQLQ
                     KGEILELLSDCSQSINNIPIDTKNHGYTLLSVEQNGTKLRYLIQR"
     gene            8439..8783
                     /locus_tag="PXB27_001558"
     CDS             8439..8783
                     /locus_tag="PXB27_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDL5346330.1"
                     /translation="MNKYALLAIFWFIIGIYALLFRESTNDIPPFPHFDKVSHFALFF
                     IQIWLSANAFIRSKKGIPYFGLIIFALLYAFTSEWAQATFTSTREGSWLDGLADMLGA
                     SLSLLLVKYCKK"
     gene            complement(8846..9793)
                     /locus_tag="PXB27_001559"
     CDS             complement(8846..9793)
                     /locus_tag="PXB27_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HDL5346331.1"
                     /translation="MNRITQILNIKHPIVQAPMSWLTDAHLVASVAEAGGLGFLAPHA
                     GQTTNPTSNEEVLDRMRNEIRKVKALTDKPFGVPFILSYDFSLIPLMVDLFIEERVPV
                     VLDNGWLDPEIYAKFKAAGIKIICRLPNPNLADALKAQALGADILVLTGFDEGGTLPM
                     KEVGSFNVLAEFVGKVDLPIMLAGGIADKKAVQAALTLGAEGVWIGTAFIATEECRAS
                     EKVKNWIVEANANDLLLFRTEPHYYRSLPTGLAKKCFEMSENGATRSEIAKVMNAGTG
                     MRLGMLEGDFDNGYVSVGNGISQINKIKSVKELIDELMA"
     gene            9976..>10777
                     /locus_tag="PXB27_001560"
     CDS             9976..>10777
                     /locus_tag="PXB27_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346332.1"
                     /translation="MSIRWENIKPLQGSQNSAFEELVCQLARQEFKSQGKFTRISAPD
                     GGIEAMCELPDGSVCGWQAKYFLSSFSSSQWQQIEDSFKESLKNYPKLTKYYVCVATD
                     RANANVSGKKSFLTKWEEYTQRWKDFAQSQGREIEFEFWGSFELSDLLSKPENAGKKF
                     FWFNESELSDKWFEQYNQQAINNLGVRYTPEINVDLPIAMQLESLARTQIFKKHLSDE
                     FHKLLVEIKSQYKSLYRYEALENHFEPVYELLIDKLNLLNINNLETTID"
BASE COUNT         3211 a         2124 c         2136 g         3306 t
ORIGIN      
        1 aatacttact tatgaaatgt atttccgccg agaaaattga ggttattcct gtcattgatc
       61 aacgcatttt gacagtttgt gatttaatct taacgaattt ggctaaaaat gagagcttag
      121 aagcaatggc agaaaaagta tttttatcgc catctcgcct ctcacatctt ttcaagcaat
      181 cgctggggat cagcttagtg caatggcggg aattacaacg catttccgaa tccaaaaaat
      241 tgctttattt ctctaacatc tctatctcta atattgcgaa atccttaggc tatgaggatt
      301 ctctctactt ttccaaaatt ttcaaaaagc ataccgggct gtcaccctct gagtttagaa
      361 ccttagaacg gcgtaaagac agtgaaatgt gatcttgatc tcactttact ttgcatagca
      421 gaattttcca tatcgataac aaaaagttac ctgtttttct ctcattcaaa agcataagat
      481 acaaacagct ttaatagtgc tgtttctatt agagaaggga tatcaagctc tgtgagtgat
      541 attcagtgaa ataacttctt atgtatgagg gaagaacaat gaaaaaacca acgttcaagc
      601 acgcattatt ttcaagtatt ttagctttag cctgttacgg ctcggcattt gccaatgatg
      661 ttgttaatat ctccaaaatt gacggtatgc cgtggtttaa tcgtatggca gagggtgttg
      721 tgcaagcagg caaagataac aatatcaatg cttatcaagt tggtccatca aataccgatg
      781 caccacaaca agtaaagtta attgaagatt taattgcgaa aaaagttagt gctatttcca
      841 tcgtccctaa cgatgcaaat gccttagaac cggtactgaa aaaagcgaaa cagaacggta
      901 ttgtggtatt aaccaatgag tcagtcggac aacctagtgc cgattgggat attgaaatta
      961 tcgataatgc acaatttgca gcagactatg tagaagaagt agcaaaagct atgggcggaa
     1021 aaggcggcta tgtgatttat gttggtggtt taaccgtgcc tcaacataat ctgtgggctg
     1081 atttattcgt gaaataccag aaagagcatt atccggatat gtttgaagtg accagcagaa
     1141 tgcctgttgc cgaaaatatt aatgatgcac gccgtaccac cattgattta gtgaaagccc
     1201 attccgatct taaagcggtc gtttcttttg gttcacaagg tcctattggt gcagggttgg
     1261 cagtgaaaga aaaacgctta aaaaataaat taaatgtctt cggaatgatg attccttccc
     1321 aagcagccaa tttaattaaa agcggtgata ttacaatggg aattacctat gacccggcaa
     1381 atgcaggtta tgctttagct gctgtcgctg ccaaagtatt aaaaggcgaa aaaattgagg
     1441 atggattaga gatcccaacg gtcggtaaag ccaaagtaga tagtgagaaa aaactacttc
     1501 aattccacaa tgtgctgaaa gtcaccaagg aaaatatcga taaattatat taatttgctt
     1561 gaaatatggg ctgatagcca gcccatattt tccctcagga ggcacttatg gcatttattt
     1621 cgatgcggca tatcagtaaa acattccacg gcataaaagc attaaatcaa gtcaatctct
     1681 cattagatta tggcgaagca ctttgtcttg ccggtcaaaa tggctgtgga aaatcaacct
     1741 tgatcaaaat tctttccggc gtttatcaac cggataaagg ggctgaaatt caaattggtg
     1801 caacccaata taccaagctg acaccacagg aatcgattga taaaggtatt caagtgattt
     1861 atcaagattt agccctcttc cccaatttaa ccgtggcaga aaatattgcc attaacctgc
     1921 accgaaaatt tggctgggtg agtcaagatc acattcatca ggttgccctc actgctattc
     1981 aaagcattaa tgcaaatcta gacctgaatg caatattaga agatttaccg atcgctcaac
     2041 aacagttagt cgctatttgt cgagcgttag cacaaaatgc cagattattg attatggacg
     2101 agccgactgc ctcacttact gcaaaagagg tcaaagatct gttagatgtc gtcatcaaac
     2161 ttaaaagcaa aggcattagc atcatctttg tcagtcataa attgcaagaa gtaatgagtg
     2221 tttccgatag cgtattagtg ctgaaagatg gcaatatggt tgggcaatat cctatccgtg
     2281 aaatgaatga aaaacgcctt ggtttcctaa tgactgggct ggaaatcgat tacaagcggt
     2341 tagatttgcc ggattttttg caaagaaaaa ccgtattgga agtccgcaat ttaacgctgc
     2401 ctcatcagta tcaagagatt aacttttcgc tacgagaagg ggaaattgtt gcccttacag
     2461 gattgctcgg ttcagggcgt actgagcttt gcctaagttt attcggcatt actcaaccac
     2521 aatccggcga tattttatta aacggtgaaa aagtgatatt taacactaac cgtgatgcca
     2581 ttgccaaagg gattgcttat gtatcggaag atagaatgca caccgggctg attatggcag
     2641 agtcgattca tcacaatatt atttcgacta tttttcacaa aatcaccgat aaattacaga
     2701 ttattcaatc ctcgaaggct tatgcaagta gccaagcctt gattgattca ttaaagatta
     2761 aagtatccga ttcagatttg cctgttaata ccctttccgg agggaacgca cagcgagttt
     2821 cgattgcaaa atggctggca atcgacccca aaattattat tttagatgcc ccgactattg
     2881 gggtcgatat cgccaataaa gaagggatct tccaaattat taaatcattg gctcagcaag
     2941 gcattgcagt tatttttgtg actgatgaag tggaagagac ctactacaac agccatcggg
     3001 taattgtaat gaaaaaaggt cgcattgttg atgaaatctt gccggcatat tccagtgaaa
     3061 aatcgattgc ggaggttatt tatgaaaatc agcaataatc atattctttc cagtacgtta
     3121 ctgattttaa tcctattatt aaccctattt actcgtgatt ttgcaactat tgagaacctt
     3181 tatgatgttg taaataatta cgcaatgtta atgattttag cttgtggttt atttgtggta
     3241 ttgctgtctg gcggcattga tatttctttc cctgctatca ctattatttc ccaatatgtt
     3301 atggtcactc tgatcggtaa atatgatgta ggatttgccg gcgtttttgt tatttcttcc
     3361 accatcggca tattattagg ttttattaat gctctatttg ttaatcggct tagtgtccct
     3421 tccattatta ttacgatctc aacactcaat attttttatg gcggactact ctattttacc
     3481 aaaggtgtat ggctttatga gtatcccgaa tggtttaccc aagaaatcgt gttctttaaa
     3541 cagataactg ctgagggagt ggaatttgga ttatcgtttc aagcattaat ggctattttg
     3601 gcggtgatat taaccggctt aattaccaat aaactcacta ttggtcgaaa aatttacgct
     3661 cttggtggta atccggatgc aacctcccgt attgggttta atctcttatt tctccatcta
     3721 tttgcatacg gttatatggg ctttatggca ggcttggcag gcatagtgca atcttatacc
     3781 gtacaatctg tcgcaccgga ttcactactg ggttatgaat taaccgtcct agctgccgtt
     3841 gtattaggtg gtgctagttt atcaggtggt agaggcaccc tgcttggtac agtaatgggc
     3901 gtaatgttac tggcgattat tcaaaacggg ctaaatttaa tgaatgtatc gtcttactgg
     3961 caaaccatta ttaccggtat catcattatc ttaagtatta gtgcaacagc cttaagtcag
     4021 cggaaaaaac aaggagcata acgatgaaaa gtaaactgga tccaacgatt ttctatctct
     4081 tagcactctt cgcagttgca gttatcgcct ttagtatttt cattccggat attttttggt
     4141 ctgtcagtaa ctttcaatct atggcatcac aaattccagt attaggtatt ttaaccctag
     4201 caatggcatt accgatgtta acaggtggaa taaatctttc cattattgcc ggaatgaatg
     4261 cttgctcatt aattattgcc tactttatta cgcaatatac cggtatttct tggttattta
     4321 ttgctatttt attaagcctt tgctgtgcct tagccattgg attactcaac ggcacattaa
     4381 ttgccattat tcgtgtttca ccaattttgg caaccttagg cacaatgact ttaattaacg
     4441 gcattaatat tctagtcact aacggttcaa ccattgcgaa ctttccggaa acaatgttga
     4501 gtattaatgc ttcagccttt ttatggattc cgctacccat tctaatcttt ctctttcttg
     4561 ctgggctgat ttggtttttc ttagaaaaaa ctgcaatggg taaagcggtt tatttcatcg
     4621 gcaataatga aaaagccacc tattattcgg gtattaatac caaaaaagtg ctgatttggg
     4681 tttatgttat ctcctcaatt ctttgtgtat ttgcagctat tttgatgatg tctaaactca
     4741 attctgcaaa agcctcttat ggtgattcct atttgttgat ctccatttta gccgcagtat
     4801 tgggcggtat taaccctgat ggaggtaaag ggaaactatt agggattatg ctcgccttag
     4861 ttttattaca agtgattgaa agtggcttaa atatgctcgg cattagcagt tacattacaa
     4921 tgattttatg gggaagctta ttgttattct ttatcttcct ccaaaaaaat cgccttttcc
     4981 gactaacaca gtaatggtgg tagattatgc aaaatgaaaa acaactcatt gaacaaggta
     5041 atactgttat tggtattgaa ttgggctcaa cccgaattaa agcggtgtta attagctcag
     5101 atgggacgat tttagcaacc ggtggggcgg attgggaaaa tcgactgatt gacggtattt
     5161 ggacctatca tcaaaatgaa atatgggaaa aactacaagc tgcttatatg gatttaagca
     5221 agacagtcaa agaaaaatat cacacaacca ttcgcacagc caaagcattg ggaattagtg
     5281 cgatgatgca cggctactta cctttcgata aacacggaaa tcagctggtt gcctttagaa
     5341 cttggcgaaa caacattacc ctaacctcat cggaaaaatt gaccgcactc tttcaatacc
     5401 caattccgca acgttggagt attgctcatt tacaccaagc attgcttaac caagagcctc
     5461 acttgccgga aattgattac ctgacaaccc tctcaggtta tgtgcattgg caactgaccg
     5521 atcacaaagt gttagggatt ggcgatgctt ccggcatgtt cccgatagat actgagttga
     5581 aatcctacaa tccaacaatg ctggctcaat ttgatcgagc cattgccgac taccgtatgc
     5641 cttggaaatt ggaagcgatt ttgccacaag ttcgtgttgc tggtgaacaa gcgggcaaat
     5701 taaccgaaaa aggtgcaaaa ttgttagatc caaccgataa tttgcaagct ggcattccac
     5761 tttgtccacc tgagggtgat gccggaacag gtatgattgc aaccaactgt atcaaagaaa
     5821 aaaccggtaa tatttctgcc ggcacctccg catttgcaat gattgtgtta gaaaaagccc
     5881 tatccaaagc ctattcagag ctggatattg tgacgacccc ggcaggtaaa ttagtggcaa
     5941 tggctcacgc taataattgc acctctgata ttaatgcgtg ggtgaattta ttcggcgaat
     6001 gcttatcggt ttttggcgta aatatctgca ccgaagaact ttatgaaatg ttatttctcc
     6061 atgccttgca aggcgatgca gattgtggag ggctattatc ctacggcttc cactccggtg
     6121 aacataatgt cggtttatcc gaaggctgtc cggttttctt gcatccaacg aatgcccatt
     6181 ttaacttagc aaactttatg cgaacccatc tctataccgc ttttggtgca atgaaattag
     6241 gaatggacat tctcattaac caagaaaaag tggaaatttc ccgtattctc ggtcacggtg
     6301 gtatatttaa aacagaaggt gttgcacaaa aaattctgtc ttccgcactg aatattccat
     6361 tggctaccgc aagtactgct tcaaatggcg gagcttgggg aattgcatta ctggcagact
     6421 ttctcagcgt atcagaccgt tacagtttag aagaatattt agataattgc attttcaaca
     6481 ccaccaaact caatctggtt caaccggata aaacaatgag tgaaggttat gaacgcttta
     6541 tgcaacgtta taagcaaggc attcctgtgg tagaaaaagc gttgttttta aatacagatt
     6601 aaataattat aatttaagta tcacaagcgg ttatttttgc aaaaaacgac cgcttgttat
     6661 ttacatttgt acaaaaaata cctactataa agacgcattt gtaaaatgtg cattgatata
     6721 tcgcaagttt tgtgtatagg aaatgaacta attactgtgt caatcagtcc ctttcttcat
     6781 cgttataccc tcttgcaaat ccgattaatc tatccttagt taaggtgttt tatgttagcg
     6841 atttggcaaa gttttaaaca aaattatcta atcaaatatt ggaatcctgt tgcagcagtg
     6901 atcgcagctg gtattatttc agcttattat ttcggcatta ctggcactta ttgggcggtt
     6961 acaggggaat ttacccgttg gggcggtcat ctgttaaatg ctttgggtgt tgatgtttcg
     7021 aactggagct attttcaact catcggctta gacggcacca tctttacccg cattgacggt
     7081 gtgatgattc tcggtatgtt tgccggctgt ctttctgccg cattatgggc gaacaacgtc
     7141 aaatggcata atcagccaca taaaaaacgg attatccaag ccttgattgg cggtgcgtta
     7201 gccggtttcg gagcccgttt agcaatgggc tgtaacctcg cctctctttt taccggtatt
     7261 ccgcaattct ccgtccacgc ttggtttttc acctttgcga ccgccatcgg cacttacttt
     7321 ggggtaaaag tgactttatt accgatgttt agagtgaaat tagagctgaa aaaaggggca
     7381 gcaaaaatca aagaaaacga tccgaaacaa gctaatcgcc gtttttgggt tggtatgctc
     7441 atttttatca cttatctttc tgcttccctt tatgtaatgc aatcctcaat caaacttggt
     7501 tttgcaatgc tttgcggctt agcctttggc ttattgattg aacgagccca aatttgcttt
     7561 acctccgctt tccgtgactt atggattacc ggacgggctt atatggcaaa agcaattatt
     7621 ttcggcatta ttgccggcac aatcggcgtg ttctcttata ttcaattagg tgtttcacct
     7681 aaaattttgt gggctggccc aaatgccatt atcggcggat tattattcgg atttggcatt
     7741 gtgcttgccg gcggctgtga aaccggctgg atgtaccgtt caatggaagg acaggttcat
     7801 tttatgtggg tcggcgtagg caatgtagtc ggttcaaccc tgctggctta ctactgaggc
     7861 gacttagctc cggtgcttac ccttgattat gaaaaaatca acctactcaa agaatttggg
     7921 ccggttggcg gattattcat caattatggc ttattaattc tttgcttaat cgctgtagtt
     7981 tggtgggaaa aacgcttcct tgccaaagcg aaagcaaaat tagcaactgc gtaatctttt
     8041 taacttcaac caacaagagg aaaatgacta tggcattcac cgttgtacaa aaactagata
     8101 aagatccaaa agacatcacg cctgattaca ccttagatac cttaggcgaa ccttgccctt
     8161 accctgcgat tacgatgtta gaaacaatgc cacagctgca aaaaggagag atattggaac
     8221 tgttaagcga ttgttcccaa tcgattaata atattccgat tgatactaaa aatcacggct
     8281 ataccctatt aagcgtggaa caaaacggca ctaaattacg ctatttaatt cagcgttaat
     8341 tgcaaaaaat tcgcacaaaa cgaccgcttg tcgtaaaata gcaactattt aagagccttg
     8401 taacgcaagg ctcttttatt ctaagaggta aaagaaaaat gaataaatac gctttacttg
     8461 caatattttg gtttattatt ggcatttatg ctttactttt tcgtgaaagc actaatgata
     8521 taccgccttt tccacatttt gataaagtaa gccattttgc cttattcttt atccagatct
     8581 ggttatctgc aaacgccttt atccgttcaa agaaagggat accttatttt gggctaataa
     8641 tttttgcctt actctatgct ttcaccagcg aatgggctca ggcaaccttc acctcaaccc
     8701 gagaaggttc ttggcttgat gggctagcag atatgctagg agcctcactt tcattattgc
     8761 tggtaaaata ttgcaaaaaa taaagcataa aagaccgctt gttatatggt ttagccatac
     8821 tacaagcggt cattttttac caaaattatg ccatcaactc atcaatcaat tctttcacac
     8881 ttttgatttt gttgatttgt gaaatgccat tgcccaccga cacatagcca ttgtcaaaat
     8941 ccccttctaa catacccaaa cgcattcctg taccagcgtt cattacctta gcaatctcac
     9001 tacgagttgc cccattttcg ctcatttcaa agcatttttt cgctaatcca gtcggcagtg
     9061 aacggtagta atgtggttcg gtacggaata acagcagatc attggcattt gcctccacaa
     9121 tccagttttt cactttttcg cttgcacggc attcttcggt ggcgataaat gccgtgccaa
     9181 tccacacgcc ttcagcccca agcgttaagg cagcctgcac cgctttttta tcggcaatac
     9241 caccagccag cattatcggc aaatccacct tgcccacaaa ttccgccagc acattaaaac
     9301 tgcctacctc tttcatcggc aaagtaccgc cctcatcaaa acccgtcagc accaaaatat
     9361 ctgctcccaa tgcttgggct tttaaagcgt ccgccaaatt gggattaggc aaacggcaga
     9421 tgattttaat gccagcagct ttgaatttgg cgtaaatctc tgggtcaagc cagccgttat
     9481 ccagcaccac aggcacacgc tcttcaatga ataaatccac cattaacgga atcagcgaaa
     9541 aatcataaga caaaataaat ggtacgccaa aaggcttatc ggtcaatgcc ttaaccttac
     9601 gaatctcgtt acgcatacgg tcaagtactt cttcgttgga agttggattg gtggtttgcc
     9661 ctgcgtgtgg ggctaaaaaa ccaagcccac cagcctcggc aacgcttgct accagatgag
     9721 cgtccgtcag ccaagacatt ggcgcttgga cgatagggtg cttgatattt aagatttggg
     9781 taatgcggtt cattggattt tcctgtttga ataaaaagaa ttggacgtag tttaatggtt
     9841 ttggatttga gagggaatgc gtgaaaatgg gaaggtggtt ttcggatttg gaaaattatc
     9901 aatgctaaaa tacttgaaat acacacgcca atcctcatat caacaaaatt gaatatttaa
     9961 ttatggtaaa agaaaatgtc tattagatgg gaaaacatca agcccttaca aggttctcaa
    10021 aatagtgctt ttgaagagct agtatgccaa ttggcacgcc aagaatttaa atctcaagga
    10081 aaatttactc gaatttctgc acctgatggt gggattgaag caatgtgtga attgccagat
    10141 ggttccgtat gtggttggca agccaaatat tttttaagct cattttcttc atcacaatgg
    10201 caacaaattg aagattcctt taaagaatct ttaaaaaact atcctaaact aaccaaatac
    10261 tatgtttgtg tggcaaccga tagagcaaat gccaatgtat ctggaaagaa atcatttctt
    10321 accaaatggg aggagtatac ccaaagatgg aaagactttg ctcaatcaca aggtcgtgaa
    10381 atagaatttg aattttgggg aagttttgaa ttatctgact tattatcaaa acctgaaaat
    10441 gcaggcaaaa aattcttttg gtttaacgaa agcgaattat ctgacaaatg gtttgaacaa
    10501 tacaatcaac aagccattaa taatcttggt gtgcgttata cacctgaaat taatgttgat
    10561 ttacctattg caatgcaatt agaaagcttg gcaagaaccc aaattttcaa aaaacatctg
    10621 agtgatgaat ttcataaatt gttagtagaa attaaatcac aatacaaatc tctttatcgt
    10681 tacgaagcgt tggaaaatca ttttgaacct gtatatgaac ttttgattga caaacttaac
    10741 ttattaaata taaataattt agaaacaaca attgata
//
LOCUS       DANLUQ010000079        10733 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.079, whole genome shotgun sequence.
ACCESSION   DANLUQ010000079 DANLUQ010000000
VERSION     DANLUQ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10733
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.079"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..858)
                     /gene="pdxY"
                     /locus_tag="PXB27_001561"
     CDS             complement(<1..858)
                     /gene="pdxY"
                     /locus_tag="PXB27_001561"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HDL5346333.1"
                     /translation="MKNILSIQSHVVYGYAGNKASTFPMQLLGVDVWALNTVQFSNHT
                     QYGKWGGMVMPKEQIGSIVDGIHNIGELHRCDAVLSGYIGSAEQVAEIIQAFHKVKAE
                     NPNAIYVCDPVMGHPDKGCIVADGVKEGLINLAMAQADIITPNLVELRELTGLTVENF
                     EQAVEAVKVILTKGPKKVLVKHLSRVGKESNKFEMLFANQDGIWHISRPLYQFAKEPV
                     GVGDLTAGLFVANLLNGKSDLEAFEHTANAVNEVMEQTARSGLYELQIIASRDRIVNP
                     QCSYQAVKIA"
     gene            977..1052
                     /locus_tag="PXB27_001562"
     tRNA            977..1052
                     /locus_tag="PXB27_001562"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1010..1012,aa:Thr,seq:tgt)
     gene            complement(1147..2100)
                     /gene="accA"
                     /locus_tag="PXB27_001563"
     CDS             complement(1147..2100)
                     /gene="accA"
                     /locus_tag="PXB27_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HDL5346334.1"
                     /translation="MSQEFLDFELPIAELEAKIESLRAVSEQDDKINLDDEIKRLQKK
                     SEELTKKTFANLDAWQISRMARHPNRPYTLDYIEHIFTEFDELAGDRAFADDKAIVGG
                     IARLDGRPVMVIGHQKGRTVKEKVKRNFGMPAPEGYRKALRLMEMAERFNMPIITFID
                     TPGAYPGIGAEERGQSEAIARNLREMSQLSVPVICTVIGEGGSGGALAIGVGDKVNML
                     QYSTYSVISPEGCASILWKSADKASTAAEVMGLTAPRLKELELIDNIVPEPLGGAHRN
                     VEQIAQNLKQRIVEDLAGLDQLNKEDLLERRYQRLMNYGYV"
     gene            complement(2154..3578)
                     /locus_tag="PXB27_001564"
     CDS             complement(2154..3578)
                     /locus_tag="PXB27_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HDL5346335.1"
                     /translation="MKKQITRRQFLQGSALAAGFATVPTPLLAAERPQLNIPPLMDIG
                     RGKPVRLDLRPAQTQFDKGKLVDVWGVNGQYLAPTVRVKSGDFVKLTYLNNLPQKISM
                     NIQGLQASSEMIGTVHRSLDPKSSWSPIVSINQPACTCWYHADTMLNSAFQVYRGLAG
                     LWMIEDEQSKKATLPNKYGVNDIPLILQDQLLNKDGVQVLDKNTSQFFGKRLFVNGQE
                     SPYFNVPRGWVRLRIVNASLSRAYELRLDNEQPLYLIATGLGMLAEPLETESVQLAPS
                     ERVEVLVDLSEGKAVSLISGQKRDIFYKIGQVFADNDELNDNVILELRPEGMAAVLNN
                     KPSLPAFNLEEFNLKISQERRFAIRPADYLINQKRFDPKRIDFTAQKGSVERWYLHST
                     QAVGFTLQGAKFIIETLNRKQYPIKQLAWRDTVWLEAEQEITLLVRFDNTAPEQLPFT
                     FGVSDFMLRDRGTMGQFSVIENGQ"
     gene            complement(3580..4314)
                     /locus_tag="PXB27_001565"
     CDS             complement(3580..4314)
                     /locus_tag="PXB27_001565"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HDL5346336.1"
                     /translation="MLKIFRILIVALAAIFISIFGTIYALLRLRHPSSVGVVARWFGA
                     LHKLVGLELITRKKPEINYPVVYIGNHQNNYDMVTISSMVAEKTVSIGKKSLIWIPFF
                     GLVYWATGNIFIHREKRSSAIETMNKAGQIVKERNVSVWMFPEGTRSRGRGLLPFKTG
                     AFHLAISAGVPIVPVVCSNLHNKVDLNRSNNGTVICEMLDPIDTSGYSRENVKELMEK
                     CHQIMLAKFEQLNQEVEQLENTAKNA"
     gene            4461..4958
                     /gene="tpx"
                     /locus_tag="PXB27_001566"
     CDS             4461..4958
                     /gene="tpx"
                     /locus_tag="PXB27_001566"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005578656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HDL5346337.1"
                     /translation="MSKVTLAGSPIDVAGTFPQKGDVVADFTLVNSDLSDVSLSDFDG
                     KRKVLNIFPSIDTGICATSVRVFNQKAADVANTVVLCISADLPFAQARFCGAEGIENA
                     KVLSSFRHRDLHNKLGVDIISGPLAGLTSRSVIVLDENNKVLHSELVPEIKQEPDYEA
                     ALAVL"
     gene            complement(5043..6668)
                     /gene="yidC"
                     /locus_tag="PXB27_001567"
     CDS             complement(5043..6668)
                     /gene="yidC"
                     /locus_tag="PXB27_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDL5346338.1"
                     /translation="MNNRGLLLMGLFLVSILIFTQWQQDFDPEIQAQKQAQVAAQTQQ
                     AGDLPSASNANNPIADSITQGKTITVESDVLRLTIDTLGGDVIGSDLLKHNAELHSNT
                     PFKLLQQDGDKIYVAQSGLVGKNGIDTNAGRPQYAVTADSFKLAEGQNEISVPLTFEK
                     EGVVYTKTFTLKRGSYDVGVNFNIKNNTAQAIEVQPYGQLKHSIVESSGNLTMPTYTG
                     GAYSSSETNYKKYSFQDMEKANLSVDTKAGWIAVLQHYFVSAWVPNQDADNSLYSRTN
                     NGVATIGYRGPLTTIAPNSEANLNSKLWTGSKDQKEMGESANHLDLTVDYGWAWFIAK
                     PLFWLLTAIQKIVSNWGLAIIGVTIVVKTILYPLTKAQYTSMAKMRMLQPRITEMRER
                     FGDDRQRMSQEMMKLYREEKVNPMGGCLPILIQMPIFIALYWTFMEAVELRHAPFFGW
                     IQDLSAQDPYYILPLLMGGSMFLLQKMSPTPVADPVQQKVMTFMPVIFTVFFLWFPSG
                     LVLYWLTSNLITIAQQWLIYRNLEKKGLHSRKK"
     gene            complement(6821..7246)
                     /locus_tag="PXB27_001568"
     CDS             complement(6821..7246)
                     /locus_tag="PXB27_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HDL5346339.1"
                     /translation="MTIWKQTATCEQLNTLSQKSAVSHLGIEFTAIGEDWIEAQLQVD
                     ERTQQPFGVLHGGVSAALAETTANAGALMVCEPHQIAVGMELNISHLKSIPAGTKAIA
                     KATPLKLGREVQVWNIDIKDEQGNLCAVARLSTKTLDKR"
     gene            complement(7575..10667)
                     /locus_tag="PXB27_001569"
     CDS             complement(7575..10667)
                     /locus_tag="PXB27_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDL5346340.1"
                     /translation="MIPRLSEVPQLDKATQSYLTELVNQHFSGEIASNYADRLSLATD
                     NSVYQQIPQAILFPKSVSDVVILTKLAQKPEYQHLTFTPRGGGTGTNGQSLNNNIIVD
                     LSRHMNQILELNVEERWVRVQAGVVKDQLNQFLKPHGLFFSPELSTSNRATLGGMINT
                     DASGQGSLQYGKTSDHILAIKSVLINGDILETQAVKSADFFAHIEGLNLSALGKTLHR
                     EIFNRCKTLRPKVLNELPQLNRFITGYDLKNVFNHGESEFNLTRILTGSEGSLAFICE
                     AKLNLLPIPKYRTLINIKYNSFDAALRSAPFMLEAKALSVETVDSKVLNLAKQDIVWH
                     SVSDLLTEDPANPILGINIVEYASSDQTQIETQVKALCQSLDQKMANNEAGIIGYQLT
                     SDVNSIEKIYAMRKKAVGLLGNAKGWAKPIAFVEDTAVPPENLADYIVEFRALLDRYN
                     LPYGMFGHVDAGVLHVRPALDLCDKAQVETFKQISDQVVELTAKYGGLIWGEHGKGMR
                     SYYGERFFGETLWQELRYIKTLFDPYNRLNPGKICTPLESETSLYPIDSQMRADFDRQ
                     IPIQVREEFKGAMNCNGNGLCFNFDVHSTMCPSMKVSGNRLYSPKGRATLVREWLRLL
                     TEQNVSPDDLIFKPRKQAVKLADFVAKIRNTLNKNKEYDFSHEVKAAMDTCLACKACA
                     SQCPIKIDVPTFRSQFNELYHSRYLRPLKDYVVSNLEFIAPLMAKQPRFFNFFSTSTF
                     AEKAANKTIGMTYLPALSVPNLQHQLVEVGYQGESLESLEQQATSGDFSAKNCKTLFI
                     VQDPFTSYYDAKVVADFIALIKKLGFKPIVLPFKPSGKAQHVKGFLHKFAKTAKNQAD
                     FLNRVAKLGLPMVAVDPALTLLYREEYKEILKEARGDFKVLLAHEWLREVIKNGELEN
                     CKKSANSDRLPWYLFAHCTESTALPSSMKEWQAIFTHFGETLLNENVGCCGMAGTFGH
                     ETKHVEMSKAIYQQSWQIKLKNKPLERCLATGYSCRSQVKRFEQQGIKHPIQALLDVI
                     "
BASE COUNT         2860 a         2293 c         2300 g         3280 t
ORIGIN      
        1 ggcaatcttt acagcttggt aactacactg tggattaaca atccgatcac gagaagcaat
       61 aatttgtaac tcataaagcc ctgatctagc ggtttgttcc attacctcat tgaccgcatt
      121 tgcagtgtgt tcaaaggctt ctagatcgga ttttccattt aataagttcg ccacaaatag
      181 ccctgcggtt aaatcgccta caccgacagg ttctttggca aattggtaga gtgggcggct
      241 gatgtgccaa atgccatctt ggttggcgaa cagcatttca aatttgttgc tctctttgcc
      301 gactcggctt aaatgtttaa ccaatacttt tttcgggcct ttggttaaaa tcactttcac
      361 tgcctcgacc gcttgctcaa aattttccac cgttaaaccg gttaattctc ttaattccac
      421 taaatttggt gtgataatgt cggcttgtgc cattgccaga ttgatcaatc cctcttttac
      481 gccgtctgcc acaatgcagc ctttgtcggg gtgtcccatt accgggtcgc atacataaat
      541 tgcgtttgga ttttccgctt tcactttatg aaaagcctga ataatttcgg ctacttgttc
      601 tgccgagccg atatagccag agagtacggc atcgcaacga tgcagttcac cgatattgtg
      661 aatgccatca acaatgctac cgatttgttc ttttggcatc accataccgc cccattttcc
      721 atattgggta tggttggaaa actgtacggt atttaacgcc catacatcaa cacctaacag
      781 ctgcatagga aaagttgaag ccttattgcc ggcatagccg tagacaacgt gcgattgtat
      841 ggatagtatg tttttcattc attcactcct ttaaaatttc aatatgtgtt gttttttagt
      901 catttgaagt tgcaaggact tgaagctcat caagaaatct ctataatccc gcctcgttct
      961 cttatccatt caatatgccg acttagctca gtaggtagag caactgactt gtaatcagta
     1021 ggtcaccagt tcgattccgg tagtcggcac catcaccttt attttctgat gagtcccctt
     1081 taatgtaaaa tggcaagatt ttgcaaattt tcacaaaaat cttaccgctt gtagggtgaa
     1141 attgaattag acgtaaccgt aattcattaa gcgttgataa cggcgttcca gtaaatcctc
     1201 tttattaagt tgatctaacc ctgccaaatc ttcaacaata cgctgtttaa ggttttgggc
     1261 gatttgttca acattgcgat gagcaccgcc taagggttcc ggcacgatgt tatcaatcag
     1321 ctcaagctct tttaaacgag gggcagttaa acccattact tctgctgccg ttgaggcttt
     1381 atcggcactt ttccacagaa tcgaggcaca gccttcaggt gaaattaccg agtaagttga
     1441 atattgcaac atattgacct tatcgcccac accgatcgct aatgcaccgc ccgagccacc
     1501 ttcgccaatt accgtacaga ttactggcac ggaaagttga gacatttcac gcaggttacg
     1561 ggcaatcgct tctgactgac cacgctcttc cgcaccaatg cccggataag ctcccggtgt
     1621 gtcgataaag gtgatgattg gcatattaaa gcgttctgcc atttccatta aacgtaacgc
     1681 tttgcgatag ccttcaggag caggcatacc aaagttacgt ttcactttct cttttacggt
     1741 acgacctttc tgatgaccga taaccatcac cggacgtccg tccagacggg caataccacc
     1801 gacaatcgct ttatcatcgg caaaggcacg gtcgcctgcc agttcgtcaa attcggtgaa
     1861 aatatgctca atataatcta aagtgtacgg gcgattgggg tggcgtgcca tacgggaaat
     1921 ttgccacgca tctaaattag caaaggtttt cttggttaat tcctcgcttt tcttttgtaa
     1981 acgcttgatt tcatcatcaa gattaatttt gtcatcttgc tcgctgactg ctcttaaaga
     2041 ctcgatcttg gcttccaatt cagcaatcgg caattcaaaa tctaaaaatt cttggctcat
     2101 tctattttcc tttttatggg caaaattatg gggcattatc gcttatcagt cggttattgt
     2161 ccattttcaa ttacgctaaa ttgacccatc gtacctctat cacgcagcat aaaatcgctc
     2221 acgccaaagg taaatggcag ttgctcaggg gcagtattat caaagcgaac caacagcgtg
     2281 atttcttgct ccgcctcgag ccaaacggtg tcccgccacg caagttgttt gatagggtat
     2341 tgcttgcggt ttagggtttc aataatgaat tttgcacctt gtagcgtaaa tccgaccgct
     2401 tgtgtgctat gtaaatacca acgctcaacg ctgccttttt gcgcggtaaa atcaatgcgt
     2461 tttgggtcaa accgcttttg gttgattaaa taatcagccg gacgaatggc aaaacgtctc
     2521 tcttggctga ttttcaggtt aaattcctct aagttgaaag ccggtaaact cggtttatta
     2581 ttcaataccg ctgccattcc ttcagggcga agctctaaaa tcacgttatc attcaattcg
     2641 tcgttgtcag caaagacctg cccgattttg taaaaaatat ctcttttttg accgcttatc
     2701 agcgataccg ctttcccttc gcttaaatcg accaacactt ccaccctttc actcggtgca
     2761 agttgaaccg attcggtttc aagtggctct gccagcattc ctaagccggt ggcgattagg
     2821 tacaacggct gctcgttatc taagcgtaat tcataagcac gggaaagtga ggcattgacg
     2881 atccgcaatc tgacccagcc acgaggcacg ttaaaataag gcgattcctg cccgttgaca
     2941 aataatcgct taccgaaaaa ttgtgaggtg tttttatcta acacctgtac gccgtctttg
     3001 ttgagcagct ggtcttgcag aattaacgga atgtcgttca cgccatattt attcggtaaa
     3061 gtggcttttt tgctctgttc atcttcaatc atccacaagc cggctaagcc acgatacact
     3121 tggaatgccg agttgagcat tgtatcggcg tgataccaac aagtacaagc cggttgattg
     3181 atagaaacaa tcggcgacca gctacttttg gggtcaaggg aacgatgcac cgtgccaatc
     3241 atctcacttg aggcttgcaa tccttgaata ttcattgaga ttttttgcgg taaattattg
     3301 agataagtta atttgacaaa atcccccgat tttacccgta cggttggggc aagatattgc
     3361 ccgttcacac cccatacatc aaccaatttg cctttatcaa attgggtttg tgcggggcgt
     3421 aaatccaacc gaaccggctt tcctcgccca atatccatta aaggcggaat gttcagttgc
     3481 ggtcgctctg ctgctaatag cggtgttggc actgttgcaa atccagccgc aagggcagag
     3541 ccttgtaaaa attggcgtcg ggtgatctgt tttttcatat taggcgtttt ttgcagtgtt
     3601 ttctaactgt tcgacttctt gattaagttg ttcaaatttt gccaacataa tttgatggca
     3661 cttttccatc agttctttga cgttttcacg ggaatagcct gaagtatcaa tcggatcaag
     3721 catttcacag attactgtgc cgttatttga acggttcaaa tccactttat tgtgtaaatt
     3781 agagcaaact accggcacaa tcggcacgcc ggcagaaatg gctaaatgga atgcaccggt
     3841 tttaaatggc agtaaaccac gcccacggct acgagtacct tccgggaaca tccacaccga
     3901 cacattgcgt tctttcacga tttgccctgc tttattcatt gtttcaatcg cactggaacg
     3961 cttttcacgg tgaataaaaa tattgccggt tgcccagtaa accaaaccga aaaacggaat
     4021 ccaaatcagg ctttttttgc cgatactcac cgtcttttct gccaccattg aggaaatcgt
     4081 taccatatcg taattgtttt gatgattgcc gatatagaca accggatagt taatttccgg
     4141 ctttttgcgg gtaattaatt ccaacccgac taatttatgc aaagcaccaa accaacgagc
     4201 taccacaccc acgctacttg ggtgacgtaa acgcagtaac gcataaatag tgccaaaaat
     4261 agaaatgaaa atagcggcaa gggcaacaat taaaatacga aaaattttta acataatgga
     4321 ataaccttaa aaataaattg tctaggagta tagcaaaata aggttatttt ctaaaggcta
     4381 tccacattaa attgatagtt tcctattttg caaaaaatcg gtatatttaa accgcttgtt
     4441 tatttgataa ggagaaaaaa atgagtaaag tgactttagc aggaagcccg attgatgttg
     4501 caggcacatt tccacaaaaa ggcgatgtgg ttgccgattt taccttagtc aacagtgatt
     4561 taagtgatgt ttcgctttcc gattttgatg gcaaacgcaa agtgttaaat attttcccaa
     4621 gtattgacac cggcatttgt gccacttctg tgcgtgtgtt taatcaaaaa gcggctgatg
     4681 tagcgaatac ggttgtcctt tgtatttcag cggatttacc ttttgcccaa gcacgtttct
     4741 gtggagcaga aggaattgaa aatgccaaag tattatccag tttccgtcat cgtgatttac
     4801 acaacaaact gggggtagat attatttcag gtccgttggc aggcttaact tcacgttcag
     4861 taattgtgtt agatgaaaac aacaaggtat tacacagcga attagtgccg gaaattaaac
     4921 aagagccgga ttatgaggcg gcgttagcgg tattataatt tgcagaatta tgctggaaaa
     4981 taaccgcttg taaagcaaca aaaagcccgt tgattcaatt caacgggctt tgtttttagt
     5041 gattatttct tacgagagtg taagcctttt ttctcaaggt tacggtaaat caaccattgc
     5101 tgtgcaatcg tgattaagtt tgaggttaac cagtaaagca ctaagcctga tgggaaccat
     5161 aagaagaaca cggtaaagat aaccggcata aaggtcatta ctttttgttg taccgggtca
     5221 gctaccggtg ttggcgacat tttttgtaac aagaacatcg aaccacccat taataaaggc
     5281 aaaatgtagt atgggtcttg tgccgataag tcttgaatcc agccgaagaa aggtgcgtgg
     5341 cgtaattcta ccgcttccat aaatgtccaa tataaagcaa tgaaaatcgg catttggatt
     5401 aaaattggta agcagccacc cattgggttc actttttctt ctctgtagag tttcatcatc
     5461 tcttggctca tacgttggcg gtcgtcgccg aaacgttcac gcatttcagt aatgcgaggt
     5521 tgtaacatac gcatttttgc catcgaggtg tattgtgctt tggttaatgg gtaaagaatg
     5581 gttttcacta caatagtgac accgataatt gccaagcccc agttgcttac gattttttga
     5641 attgcggtta gtaaccagaa aagcggttta gcgataaacc acgcccagcc gtaatctacg
     5701 gttaagtcta ggtgatttgc tgattcgccc atttctttct gatctttaga acctgtccaa
     5761 agtttgctat ttaaattagc ctctgaattt ggcgcaatcg tagttaaagg gccacggtaa
     5821 ccgatagttg ctaccccatt attggtgcgt gaataaaggc tgttatccgc atcttggttt
     5881 ggcacccaag cagacacgaa atagtgctgt aataccgcaa tccaacccgc tttggtgtcc
     5941 acgcttaagt tggctttttc catatcttgg aaactgtatt ttttgtagtt ggtttctgaa
     6001 gaagaataag ccccgccggt gtaagtcggc atggttaaat tgccggaact ttcaactata
     6061 gagtgtttta attgcccgta aggctgaact tcaatcgctt gtgcagtatt atttttgata
     6121 ttgaagttta ccccaacatc gtagctacca cgttttagcg tgaaagtttt ggtgtaaacc
     6181 acaccctctt tttcaaaggt taatggtaca gaaatttcat tttgcccttc ggctaatttg
     6241 aagctatctg ccgttaccgc atattgtggg cgaccggcat tggtatcaat cccgttttta
     6301 cccactaaac cgctttgagc aacgtagatt ttgtcgccgt cttgctgtaa tagtttaaat
     6361 ggcgtgttag agtgtaattc agcattgtgt tttaacaaat ctgagccgat gacatcaccg
     6421 cctaaggtat caatagtcag acgtaataca tcgctttcca cggtaatggt tttaccttgc
     6481 gtgatgctat ctgcgattgg attattggca ttggaagcag acggaagatc acccgcttgt
     6541 tgggtttgtg ctgccacttg agcttgtttt tgtgcttgaa tttcaggatc aaaatcttgt
     6601 tgccattgtg taaaaatcaa aatagacaca agaaataaac ccattagcag taagccacga
     6661 ttattcatgt tggaattctc ttgttagtaa tggataaaaa gaaaggtatt ttataatagg
     6721 ttagcttgtt ttccaaacgc cttttatgaa caagcggtcg gattttccaa gttttttgca
     6781 aaattttcgg agaatttgac cgcttgtgga gaataagaaa ctagcgttta tctaaggttt
     6841 tggtggaaag gcgagcaact gcacacaggt taccttgttc gtctttaatg tcgatattcc
     6901 aaacctgtac ttcacgccct aatttcagcg gtgtagcttt ggcaatcgct tttgtgcctg
     6961 ccggaatcga ttttaaatgg ctgatgttta gctccattcc caccgcaatt tgatggggtt
     7021 cacacaccat taacgcccct gcattggcag tagtttccgc gagtgctgcc gatacaccgc
     7081 cgtgcaatac gccaaacggc tgttgagtgc gttcgtccac ctgaagctgt gcttcaatcc
     7141 aatcttcacc gatagcggta aattcaatcc ctaagtgtga caccgccgat ttttggctga
     7201 gtgtgtttaa ttgttcacaa gttgctgttt gcttccaaat ggtcattatt atttcctgtc
     7261 taataaaaaa gctgtctatt tagacagctt tcattatctc tttgagcgaa acatactacg
     7321 ccaatagtac attgtttggt cgcccatatt accttaagcc atatagcact aaggaggtga
     7381 tttatgtgag aaaatcagaa gttttctttc tggttgcgtt gctaatatta ttagcaactt
     7441 ccgctagcat taaagtactc accatacttg atgtgctagt ttggttattc gctagataac
     7501 ctaaaatcag acagaggcag gctggttacc tgcctcgctt tttattctaa gtacttttag
     7561 gttacatttc aaccttaaat cacgtccagc aacgcttgaa ttgggtgttt aatgccttgt
     7621 tgttcaaaac gtttgacttg cgagcggcag gagtagccgg ttgctaggca acgctcaagc
     7681 ggtttatttt ttaatttaat ttgccatgat tgctgataaa tcgctttcga catttcaacg
     7741 tgtttggttt cgtgtccgaa cgtgcctgcc attccacagc aaccgacatt ttcattgagt
     7801 aaggtttcac caaaatgggt gaaaatagct tgccactctt tcattgagct tggcaaggcg
     7861 gtggattcgg tgcagtgagc aaataaatac cacggcaagc ggtcggaatt tgccgatttt
     7921 ttgcaatttt ccagctcgcc gtttttaatc acttccctta accattcgtg tgccaataag
     7981 actttgaaat ccccccgagc ttctttgagg atctctttgt attcttcacg ataaagcaaa
     8041 gttaaggcag gatcgacagc caccatcggt agccccaatt tcgccacacg gtttaaaaaa
     8101 tcggcttggt ttttcgctgt tttcgcaaat ttatgcaaaa agcctttaac gtgttgtgct
     8161 ttaccgcttg gcttgaatgg caacacaatc ggcttaaagc ctaatttttt aatgagagcg
     8221 ataaagtctg ccaccacttt ggcatcgtaa taagaggtaa acggatcttg cacaataaac
     8281 aaggttttgc aattttttgc agaaaaatca ccgcttgtag cttgttgttc aaggctttct
     8341 aagctctcgc cttggtagcc gacttccacc aactgatgtt gcagatttgg cactgacaat
     8401 gccggcaaat aggtcattcc aatggttttg tttgcagctt tttcggcaaa agttgaggtg
     8461 ctgaaaaagt taaaaaaacg tggttgtttc gccattaaag gggcgataaa ttctaagttt
     8521 gagaccacat aatctttcag tggtcgcaaa tagcggctgt ggtaaagctc gttaaattgc
     8581 gatctgaacg tcggtacgtc aattttgatt gggcattggc tggcacaggc tttgcaggca
     8641 aggcaggtgt ccatcgccgc tttgacttcg tgtgaaaaat catactcttt gtttttattg
     8701 agcgtgttgc gaatttttgc aacaaaatca gctaatttga ccgcttgttt gcgaggtttg
     8761 aagatcaaat cgtccggcga aacgttttgt tcggtaagta atctgagcca ttcacgcaca
     8821 agagtagccc gccctttcgg tgagtaaaga cggttgcccg agactttcat tgaagggcac
     8881 atcgtgctgt gtacatcaaa gttaaaacat agcccgttgc cgttacaatt cattgctcct
     8941 ttaaattctt ctcgcacttg gattggaatt tggcggtcaa aatcggctcg catttgtgag
     9001 tcaatcgggt agagcgaggt ttcgctttct aatggtgtac aaattttacc tgggttcaag
     9061 cgattgtacg ggtcaaataa ggttttgatg taacggagtt cttgccacaa agtttcgcca
     9121 aagaaacgtt cgccgtagta agaacgcata cctttgccgt gttcgcccca aatcaagcca
     9181 ccgtatttgg cggtgagttc gactacttgg tcggaaattt gcttaaaggt ttccacttgg
     9241 gctttgtcgc ataagtcaag agcagggcga acgtgtagca cgccggcatc aacgtggccg
     9301 aacattccgt aaggcagatt atagcgatct aataaagctc gaaattccac gatgtaatcg
     9361 gcaaggtttt ccggtggcac agcggtgtct tctacaaagg cgatcggttt tgcccagcct
     9421 ttggcattgc ctagcaggcc tacggctttt ttccgcattg cgtagatttt ctcgatagaa
     9481 tttacatcac tcgtgagttg gtagccgata atcccggctt cattatttgc cattttttga
     9541 tcaaggcttt ggcagagtgc tttcacttgg gtttcaattt gagtctgatc gcttgaggca
     9601 tattcaacaa tgttaatccc taaaatcggg ttggctgggt cttcggttaa taaatctgac
     9661 accgaatgcc agacgatatc ttgcttggca agattgagca ctttggaatc aaccgtttcc
     9721 accgataagg cttttgcctc taacataaaa ggagcggagc gtaaggcggc atcaaaggaa
     9781 ttatatttaa tattaattaa ggtgcgatat ttgggaatcg gcagtaaatt cagtttggct
     9841 tcgcaaataa aggcaagcga accttcagaa ccggttaaaa tacgggtaag gttaaattcg
     9901 ctttcaccat ggttaaacac attttttaaa tcatagccgg taataaaacg gttaagctga
     9961 ggcagttcgt tcagcacttt ggggcgtaat gttttgcaac ggttgaaaat ttcacggtgc
    10021 aaggttttgc cgagagcgga gagatttaac ccttcaatat gtgcaaaaaa atcggcagat
    10081 ttgaccgctt gtgtctctaa aatatcgccg ttaataagca cagatttgat ggcaagaata
    10141 tgatcagatg ttttgccata ttgcaaagaa ccttgcccag aggcatcggt gttgatcatt
    10201 ccgcctaaag tcgctcggtt actggtagaa agttcggggg aaaagaaaag tccatgtggt
    10261 tttaaaaatt gattgagctg gtctttcacc acgccagcct gcactcgaac ccaacgctct
    10321 tccacattca gttccaaaat ctgattcata tggcgggaga gatcaacaat aatattattg
    10381 ttaagagatt gcccgttagt gcctgtgcca ccgcctcgtg gagtaaaagt taaatgttga
    10441 tattctggct tttgggcaag tttggttaga attacgacat cagaaacgga tttcggaaac
    10501 agaatagctt gcggaatttg ctgatacacg ctgttgtctg ttgccagtga aagcctatct
    10561 gcataatttg atgcaatttc cccactaaaa tgctgattga cgagttcagt taaataggat
    10621 tgggtggctt tatccagttg cggtacttca gagagtctag gtatcataag ctgtgtttgg
    10681 ttttttataa taaaagcact aaattttgct tatcttagca atttttctaa aaa
//
LOCUS       DANLUQ010000080        10571 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.080, whole genome shotgun sequence.
ACCESSION   DANLUQ010000080 DANLUQ010000000
VERSION     DANLUQ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10571
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.080"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(162..959)
                     /locus_tag="PXB27_001570"
     CDS             complement(162..959)
                     /locus_tag="PXB27_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HDL5346341.1"
                     /translation="MENFEKQFISLFQRIVPHYRRSEVFYDFITLSALDMYLVTYRDQ
                     AEPNLRERFAHAKARYSDSEFTQLAELLAVTVNALTQKRYDFLGSVFMNLNLGDGYRG
                     QYFTPSHIADLMAKVTLQDCDRIISQQGFVTLSEPTCGSGVMVIGCVNAMFDAKYNPQ
                     QQLWVHCQDVDFTAAMMCYIQLSLLHIPACIVVGDTLLNETKIQMYTLAHLMGNWSHK
                     LEQQIAPLVNSTTEKGCGNNIEKSVIVEDLSQISILDEIDDNELIFY"
     gene            complement(1032..1778)
                     /locus_tag="PXB27_001571"
     CDS             complement(1032..1778)
                     /locus_tag="PXB27_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346342.1"
                     /translation="MPNWCENKLQIFCDCIQAKQIIPKLFRKTESDKWCLDFELLVPM
                     PESICIESSSLGDRAMEFLKISPNRRLTRPLLKKYIYGSEIDRIYAKAKHHRWDVGHF
                     INWLIKRPEQQSILWLNLALGQQYLSNLAQHGYKDWYDWSNEQWGCKWNVCSDSCQVS
                     FNDNGSINCLFDTPWGPPDGWFQALCTAFPDIEFILSFFEPGMWFAGKYIANLDGSYC
                     NIYIDDRGIRSFAEEVFNEEFEDEFEEKLE"
     gene            complement(1918..2892)
                     /locus_tag="PXB27_001572"
     CDS             complement(1918..2892)
                     /locus_tag="PXB27_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1738 domain-containing protein"
                     /protein_id="HDL5346343.1"
                     /translation="MNTQTKFDLYQHITDRIVAALEQGTAPWLKPWSHPECNMALPRN
                     AVSNRLYSGINTLLLWIATAEYGYKQSKWITAKKANELGGYIRKGEKATVVMNYRPDK
                     REKLDEEGNIVYDEEGNPEIEQHFFIKKHNLFNLDQCENLPEELYDPISEPKQPTSDP
                     NQYEIFADIRQIIKGMDLHVEMKPSNRAYYQPSADLVVMPEIKQFENAQAFHSVLLHE
                     MTHATGAAKRLDREGITSGKAKFGNKVYAFEELVAEMGGAFLCAYLGFDTVPQNAAYI
                     ESWIRVIKEDKRAIVRASGKAREACQYMLDALNISQLADKNEVFEEAA"
     gene            complement(3137..3544)
                     /locus_tag="PXB27_001573"
     CDS             complement(3137..3544)
                     /locus_tag="PXB27_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
                     /protein_id="HDL5346344.1"
                     /translation="MKHDLDLLKYIWDNRNLNNKAYQYLFHICIEPMRKGDWNRYTIW
                     LNNNGNLVNISAVVYEIVSSQQLKDDIKLWQDMQFHCRPVKVETTGFISFLLHQFFNG
                     ITHVLLEQGMITETEAAEKSYHFIAANSFQRLL"
     gene            complement(3660..4502)
                     /locus_tag="PXB27_001574"
     CDS             complement(3660..4502)
                     /locus_tag="PXB27_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346345.1"
                     /translation="MSGSVLNTYFKHALVKAGFPNCLNIEYSLSHCQGDGVAFYGRLN
                     TETLINLFNKLNPQKRTQKMFSNLLNHIESWEPHYGVDFEIIRNSFGYRYSHCNTMEL
                     SVRTADDLAFFYDRDARKEWYFPTSKVEKYKALWDGFIQDLGQYIKDTSCALESNGYQ
                     IIEASPYQTETIFKFNTENYLIELKRKSSEFYSESVDWLCGDVCDFEAMIEEILQGKL
                     CYADFVATVTDKESGIQLGSDEITGVSYSPTDHSLSGYRQELISNAIASARSNAARFA
                     YLHA"
     gene            complement(4580..4975)
                     /locus_tag="PXB27_001575"
     CDS             complement(4580..4975)
                     /locus_tag="PXB27_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346346.1"
                     /translation="MARKKIQYTETDLHLYSVLWNLRHAHDLFSVKLERETKFGKAFS
                     VFSRFDVCPSYGEVDITYLVVKAGIRKTLRGFAYVGGNGFSHSRHLMSTFMYLVREIL
                     VEHNWISETELYDQPISVYKFRAINFDKW"
     gene            complement(5074..5319)
                     /locus_tag="PXB27_001576"
     CDS             complement(5074..5319)
                     /locus_tag="PXB27_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
                     /protein_id="HDL5346347.1"
                     /translation="MLVHIRLSCIGDVLPTKLNEWTSEHQALQQEISHRALEAWQNRD
                     KDIKSVRFMQQMPSRQGLNFRFLSVSEQNGKLIVSRA"
     gene            complement(5391..5744)
                     /locus_tag="PXB27_001577"
     CDS             complement(5391..5744)
                     /locus_tag="PXB27_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346348.1"
                     /translation="MSQQTAGWDVIPFESSKTVKPSLFWKLGNKLGNIFVSAKHKAKS
                     KYVEVTKGINQRIAEEDEMRALELQMCLDEQAAEYEQEISRIKRKMFFWTLLAAIIAF
                     VCGMAAVFGTQLSLI"
     gene            6118..6561
                     /locus_tag="PXB27_001578"
     CDS             6118..6561
                     /locus_tag="PXB27_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03757 family integrating conjugative element
                     protein"
                     /protein_id="HDL5346349.1"
                     /translation="MKVSRFLGYCSIVTGVFSPLAQAQPNIIVYTTKMYPIQNSQLAS
                     QIYYLDQVENFEESISRNLSKSPEIAEQEMRAFFNSADWKAQEQQLKDAYQGVISGWQ
                     NGIKKVPAVFFDNPQSSPSVIYGTTDVQKALDEWHKWVKQQGESK"
     gene            6558..7499
                     /locus_tag="PXB27_001579"
     CDS             6558..7499
                     /locus_tag="PXB27_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HDL5346350.1"
                     /translation="MKKTFLTILISASLAVPTTLYPQPARASLNTASIAASSASTSCL
                     EYKVVGTCFWLFCTYFGCKVRTSTKIKHYLPEMVVSSYNHDGQTPWTELQALNPGVKG
                     GEYRSPQRQYSQLTFKNADAFGHPQGAIGQLLGNTGYYCKTQTTPFMPYFLSGLDFFA
                     WRENMPEMFYPEALTPGMREVGRVGDSWGNIYPRSGWVTQAHDYKASATIVQRVADIV
                     SRTAQPHIYFPAAKTGGAGEWPPGPVEEGQARTHKWQILYPQMSNTCSVFPNGGQTDT
                     YSSQISNQGDYAWALWRPYSCCKRRGQTFLYSIDWSH"
     gene            7517..9529
                     /locus_tag="PXB27_001580"
     CDS             7517..9529
                     /locus_tag="PXB27_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HDL5346351.1"
                     /translation="MKSHFNFKKSHLRITTALLSSVSGLSYADSITDKLEFSKSDSII
                     SDRVFYQIGGGMGYMAPPSRGNTKVVDLGIGWKANLTCGNFDIKTTIKNDLNGLKDGF
                     KDLMGNVIDSAKGAVASMPAMVLQRANPQLYDLLTNGMYQGKLDFNRLKTSCEEMSKV
                     LADHTLGGKWGQAADLESYKQITSSQPDAKQAQKDLEDKKGKDGRVWIGGQKKGGENQ
                     EAINVVGDVVKAGFNLLNKRNALDTSTISGSSCDGNLCQTWDNPQTAADFLTKIIGEQ
                     SISTCKEDCGTKTSSKAGVGLSPEIEEEHVRTIVKLENVLNMDVPKAELLADLSSTTV
                     PVTRGLIESLRDDPDVEVLSQRLSAEIATSKIMEKMLLARRAVLAGMREPNVAQNADA
                     QVELERVLNIIDREISQVKLEMDMQKMITGNTASVILQSKSEREALAGAHSLSQDSLD
                     KRVQGLENGNVALTDLEAGSNINLSRRNIALSIPNIPNVAALIPNLPNRGGSGVGGNF
                     PSLAQISGSTMDQATGLLRNFEGFISKAQWDVNAHRVGYGSDTITRADGTIVKVTKDT
                     VITKEDAERDLARRTAIFANNVRKELGDSNWNALPPNAQAVLVSYAYNYGSLAKTKSV
                     LDAARRSAQSGDMNALATAIRNRQVDNNGINARRRNQEADYILGKN"
     gene            9550..9978
                     /locus_tag="PXB27_001581"
     CDS             9550..9978
                     /locus_tag="PXB27_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346352.1"
                     /translation="MRKLLGITVLTLCTTSAFATDKNVVFSCTSTEGKPLTVKRVGND
                     YEYSYDKTIFKNPIKKAVTNDGSIIARGSGFTTYALELKNDGLKYVVGFVQPNGNSKE
                     FIEPGATISRDNDQPSIGSVDCDPYKKIYYKFDVHLMNTL"
     gene            10194..>10571
                     /locus_tag="PXB27_001582"
     CDS             10194..>10571
                     /locus_tag="PXB27_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346353.1"
                     /translation="MDITTQNIELRYLSEQFYLDVAPHLKEILLKETRPYCVLLVRVK
                     NLDFALPLRSNLPEREGVGIKTIPNPQEYGKFKGIDFSKAILLTDKSYLKTDTVQLKN
                     KSELLNIQGNERRIVREFKKYVSE"
BASE COUNT         3354 a         2052 c         2025 g         3140 t
ORIGIN      
        1 atatagactt taaatgctat tgacacaatt cacagagtta aattaaggta gtaaatgctg
       61 tcggcaatat cggttggtat caatagtcaa atatttattg aaaattcagt tgctgatata
      121 aaataattta gccaccttat ttctaaggcg gctattattt gttagtaaaa tattagctca
      181 ttatcatcaa tttcatctaa aattgagatt tgagataaat cttccactat cactgatttt
      241 tctatattgt taccacagcc tttctcagta gtgctattta caagtggtgc aatttgttgc
      301 tccagtttat gagaccaatt tcccatcaaa tgagccagcg tatacatttg aatttttgtt
      361 tcattcaata gcgtatcacc aactacaata caagcaggaa tatgtaataa cgatagctgg
      421 atataacaca tcatggcagc tgtaaaatca acatcttgac aatgaaccca aagttgttgt
      481 tggggattgt atttcgcatc aaacatcgca ttaacacaac caatcaccat tacacccgaa
      541 ccacaagttg gttctgataa agtaacaaat ccctgttgtg agatgattct atcacagtcc
      601 tgtagtgtaa ctttcgccat tagatctgcg atgtgagatg gtgtaaagta ttgccctcta
      661 taaccatcac caagattgag attcataaat actgagccta agaaatcata gcgtttttgt
      721 gttaaagcat tcaccgtaac agctaacaat tctgctaatt gagtaaactc agaatctgaa
      781 tatcgtgcct tagcatgagc aaagcgttct cgcaaattag gttctgcttg atctcgatac
      841 gttactaggt acatatcaag tgcagagagt gtaataaaat cataaaacac ttctgaacgt
      901 cgataatgtg gtacgatacg ttgaaataag ctgataaatt gcttttcaaa gttttccatt
      961 agactactcc ttaaagttta cggagtaatc cccctaggga attaccccta gggtggaaaa
     1021 attaattaag gctattcaag tttctcttcg aactcatctt caaattcttc attaaagact
     1081 tcctcagcaa aactgcgaat acccctatca tctatgtaaa tattgcagta tgagccatca
     1141 agatttgcga tatatttgcc cgcaaaccac attcccggct caaaaaatga aagtataaac
     1201 tcaatgtcag gaaaggcagt acaaagtgct tggaaccaac catcaggtgg tccccatggc
     1261 gtatcaaata ggcaattaat agatccatta tcattaaagc taacttgaca gctatctgaa
     1321 catacattcc atttacagcc ccactgttca tttgaccagt cataccaatc tttgtaacca
     1381 tgttgagcta ggttagacag atattgctgg cctaatgcga ggtttagcca taatatactt
     1441 tgttgttcag gacgttttat caaccagttg ataaaatgac ctacatccca gcgatgatgc
     1501 tttgccttag catatatccg atcaatttcg gacccataga tatacttttt gagtagaggg
     1561 cgtgttaaac gtctatttgg tgaaattttt agaaattcca ttgctctatc accaagacta
     1621 cttgattcaa tgcagattga ttctggcatt ggaacgagta attcaaagtc taaacaccat
     1681 ttatcacttt cagtttttct gaaaagctta ggaataattt gtttagcttg gatacaatcg
     1741 caaaaaattt gtagtttatt ttcacaccag tttggcataa tgtactccta aataaagtag
     1801 gggtacactt gccttgaggc aatgttcccc aagggttaaa agataatcga tacataattc
     1861 ctaaaggatt atgtattact atgaaagatt gtgttgaaag tacctttcag caaagagtta
     1921 agctgcttct tcaaagacct cgtttttatc agccagctgt gagatattta atgcatctaa
     1981 catatattga caagcttcac gagctttacc agaagcccgg acaatagccc gtttatcttc
     2041 ttttataact cttatccaac tttcaatata tgccgcattt tgaggtaccg tatcaaatcc
     2101 aaggtaggca cacaaaaatg ctccccccat ttcggcaacc aactcctcaa aagcatagac
     2161 tttgtttcca aattttgctt ttccggaggt aataccctca cgatcgagcc ttttagcagc
     2221 accagttgca tgagtcattt cgtgcaataa cacactatga aacgcttgag cattttcaaa
     2281 ctgttttatt tccggcatta caactaaatc tgcactgggt tgataatatg cccgattact
     2341 cggtttcatt tccacgtgta aatccattcc tttgataatc tgccgaatat cagcaaaaat
     2401 ctcgtattgg tttggatccg atgtaggctg tttaggttct gatataggat cgtacagttc
     2461 ctcgggtaaa ttttcacact ggtctagatt gaacagattg tgcttcttaa tgaaaaagtg
     2521 ttgttcaatt tctggattac cttcctcgtc atacacaata tttccctcct catcaagttt
     2581 ttctctctta tcaggacggt agttcattac aacagtagct ttttcacctt tacggatata
     2641 tccgccgagc tcgttagctt tttttgctgt aatccactta ctctgtttgt aaccgtattc
     2701 tgcggtagca atccagagta atagtgtgtt gataccagaa tacaatcggt tagagaccgc
     2761 attacgaggt aatgccatat tacattctgg atgactccac ggttttaacc atggtgcagt
     2821 cccctgttcc agtgctgcaa caattcggtc tgtgatatgt tggtataaat caaatttagt
     2881 ttgagtgttc attagtgttt ctccaaagtt caggtagaaa atggggaaac actattaccc
     2941 ttatcgggca ctagtttccc cgatgggtgg tttaaaatct caacagagat agtttttcta
     3001 ggttcatcaa aaccagtaga actgcctata atgcagtaac tacagataat ttgttaatca
     3061 gtattgattc aagttaaaca taatcctcat atcgaaaatt actttggtaa tacaaagtta
     3121 cattatttca gatccattaa agtaaacgtt gaaagctatt agcagcaata aagtgataac
     3181 ttttttccgc agcttctgtt tccgtaatca ttccctgctc cagcaaaaca tgggtgattc
     3241 cattgaaaaa ctgatgtaat agaaatgaaa taaaacctgt tgtttcaact tttactgggc
     3301 gacaatgaaa ctgcatatcc tgccaaagtt taatatcatc ttttaactgt tgagagctca
     3361 caatttcata tacaacagca gaaatattaa ctaaattacc gttgttattc agccaaattg
     3421 tataacgatt ccaatctcct tttctcattg gctctataca aatgtgaaat aggtattggt
     3481 aggctttatt atttaaattg cggttatccc agatgtattt gagtaaatct aaatcgtgtt
     3541 tcataatgtt tctccaaaag aaaaaagtgg agaaacatct tacctatagg gaaaatattt
     3601 ccccacaggg taaaaaacaa tctcgataag cggtcaaatt gaaataaaaa acctacaaat
     3661 caggcatgca agtaggcaaa acgtgcggca ttactacgag cagaagctat agcattacta
     3721 ataagctctt gtcgataacc actcaatgaa tggtctgtcg gcgaatagct tacgcctgta
     3781 atttcatcgc tacctagttg aatgccactt tccttatcag taacggttgc tacaaaatca
     3841 gcatagcaaa gtttcccttg taggatttcc tctatcattg cctcaaaatc gcagacatca
     3901 ccacatagcc aatcaacaga ctctgaataa aattcagagg atttacgttt aagttctatc
     3961 agatagtttt ccgtgttaaa tttgaaaata gtttccgtct gatacggtga ggcctcaata
     4021 atctgatagc cattcgattc taaggcacag gaagtatctt taatatactg acctaaatct
     4081 tgaataaatc catcccacag ggctttgtat ttttccactt ttgatgtagg gaaataccac
     4141 tcttttcggg catcacgatc atagaaaaat gccaaatcat ctgctgttct aacagaaagc
     4201 tccatcgtat tacaatgcga atagcggtac ccaaaactat ttcgtatgat ttcaaaatcc
     4261 acgccatagt gaggctccca agattcaatg tgattcagca gattagaaaa cattttttgt
     4321 gtgcgtttct gaggatttag cttgttgaaa agattaatta acgtctccgt attaagccta
     4381 ccgtagaatg ctacgccatc gccttgacaa tgagaaagtg aatactcaat gtttaagcaa
     4441 ttaggaaaac cagctttaac aagagcgtgt ttaaaataag tgtttaagac tgagccagac
     4501 attgcgcttc tcctaaaatt agagggaaac acattaccct tttccggaaa ttgtgtttcc
     4561 cgaaagggtg aaagattgat taccacttat cgaagttaat cgctctgaat ttataaacgg
     4621 atataggttg atcatagagt tctgtttccg aaatccaatt gtgttcaacc aagatttctc
     4681 tgacaagata cataaatgta ctcatcaaat gacgactatg actaaacccg tttccaccaa
     4741 cataagcaaa ccctctcagg gttttgcgaa ttcctgcttt aacaacaagg taagtaatat
     4801 caacctcgcc ataagacggg cagacatcaa agcgagaaaa aacagaaaat gctttgccaa
     4861 acttggtctc acgctctaat ttcacactga atagatcatg tgcatggcgt aaattccaaa
     4921 gtacactgta taagtgcaaa tctgtttcgg tatattgaat ttttttacga gccataatgt
     4981 ttctcctaaa aaataaaagg gaaacattac ttccctctgg ggataatgcc tccccttgtg
     5041 aggtataaga ataggatgtg aatatgaagt tgcctatgct cgactaacaa ttagcttgcc
     5101 gttctgctcg gacacactca gaaagcgaaa attaagtcct tgccgagacg gcatttgttg
     5161 cataaatcgc acgcttttga tgtccttatc tcgattttgc cacgcttcta aagctctatg
     5221 actaatttct tgctgtaagg cttgatgctc tgatgtccat tcattaagtt tagttggtaa
     5281 aacatctcca atacaggaaa gtctaatatg aactaacata catttctcca gaataagggg
     5341 aaatgcattc cccttaaggg aaacaacatt tccccgatgg gtgagttaaa ttagattaaa
     5401 gagagctgag taccaaaaac ggcagccata ccacatacaa aggcaataat ggcagccaac
     5461 aaagtccaga aaaacatctt acgcttaatg cgggaaattt cctgttcata ttcggctgct
     5521 tgttcgtcta agcacatttg cagttctaag gctcgcattt catcttcttc agcaatccgt
     5581 tgattgatgc ctttggtgac ttcaacatac ttgcttttgg ctttatgttt cgctgaaaca
     5641 aaaatattac ctagcttgtt gccaagtttc caaaaaagag aaggttttac ggttttggat
     5701 gattcaaacg ggataacatc ccatcctgcg gtttgttgac tcatagcgag ttctcctaaa
     5761 agttaagtga ataaaattcc tcaggagatc gcaattccaa gggaattgaa atatccctcg
     5821 gaaggtaggt gtgtaggtag cattcctgca aatttgcaga gttccctttc agaatgctgg
     5881 gagacatttt gggtaaataa ctcaactacc tctaaggata gccgccgctt ttcaagagtt
     5941 atgcgacttt taagggctgt atctacagcg tcttaatgct taatatcata cgaattcctc
     6001 caataaaatc aaccgttttt ttgttcaaaa aatatctctt ttttgaaaag agtttttcct
     6061 atcctcaaca taaaaatctc gctaattttc tgcttatttg ttcgggagga gagcgtgatg
     6121 aaagtaagta gatttctggg atattgcagt atagtaacag gtgtgttttc gccccttgca
     6181 caagcacaac ccaatatcat cgtctatacc acaaagatgt accctattca aaattcacaa
     6241 cttgcttccc agatttatta tcttgaccaa gtagagaatt ttgaagagtc aatcagtcga
     6301 aacctcagta aatcaccaga gattgccgaa caagaaatgc gtgcattctt taattcagca
     6361 gattggaaag cccaagagca acaattaaaa gacgcttatc aaggcgtgat cagtggatgg
     6421 caaaatggca ttaagaaagt acctgcggtg ttttttgata atccacaatc ttctccgagt
     6481 gtgatttatg gtacgactga cgtacagaaa gcattagatg aatggcataa atgggttaaa
     6541 caacaaggtg aatcaaaatg aaaaaaacgt ttcttacgat actgatctct gccagtttag
     6601 ctgtcccaac aaccttatat cctcaaccag ccagagcctc attgaatacc gcttccattg
     6661 ctgcatcaag tgcctcaaca agctgtttgg aatataaagt tgttggaacc tgtttttggt
     6721 tattttgtac ctactttgga tgtaaggttc gcacatcaac caaaattaag cattatttac
     6781 ctgaaatggt tgtttcatct tataaccacg atgggcaaac gccatggaca gaattacaag
     6841 ctctcaatcc tggtgtaaaa ggtggagaat accgttctcc acaacgccaa tactctcaac
     6901 tcactttcaa aaatgcggat gcgtttggac acccacaagg agctatcggt caattattag
     6961 gaaatacggg gtactattgt aaaactcaaa ctacgccatt tatgccttac tttttgagtg
     7021 ggctggattt ttttgcctgg cgtgaaaata tgccggaaat gttttacccg gaagctctga
     7081 ctccgggaat gcgggaagtt ggccgtgtag gcgatagctg gggaaatatt tacccacgtt
     7141 ccggttgggt aacccaagca cacgattata aagcctctgc gaccattgtg cagagagtgg
     7201 cagacattgt gagccgaaca gctcagccac acatttattt cccagcagcc aaaacaggtg
     7261 gtgcaggaga atggcctcca ggaccggtcg aagagggaca agcaagaaca cataaatggc
     7321 aaatacttta ccctcaaatg tcaaatacct gctctgtttt tcctaatgga gggcaaacag
     7381 atacctattc ttctcaaatt tcaaatcaag gtgattatgc ttgggcatta tggcgacctt
     7441 atagttgttg caaacgccgt gggcaaacat tcctttacag tattgattgg tcacactaat
     7501 aacattggga gcaataatga aatctcactt taacttcaaa aaatcccact tacggatcac
     7561 gacagcatta ttatcaagtg tatcaggact atcttatgcc gattcgatta cggataaact
     7621 ggaatttagt aagtcggatt cgattatttc agaccgtgtt ttttaccaaa tcggcggtgg
     7681 aatgggttat atggctccac ctagtcgtgg taataccaaa gttgttgatt taggaattgg
     7741 ttggaaagcg aatttaacct gtggtaattt tgatattaaa acgaccatta aaaatgattt
     7801 aaacggtctc aaagacggct ttaaagattt aatggggaat gtcattgatt cagcgaaagg
     7861 tgcggttgca agtatgcctg caatggtatt gcaacgggcc aaccctcaac tctacgattt
     7921 actcacaaat ggtatgtacc aaggtaagct ggatttcaac cgtcttaaaa ccagttgtga
     7981 agaaatgtcc aaagttttag cagatcatac actgggagga aaatgggggc aagcagctga
     8041 tttagaaagc tataagcaaa ttacctcctc tcaacctgat gcaaaacaag ctcaaaaaga
     8101 cctggaagat aaaaaaggga aagatgggcg agtttggata ggaggtcaga aaaaaggtgg
     8161 ggaaaaccaa gaagctatca atgttgttgg cgatgttgtc aaagctgggt ttaatttact
     8221 gaacaaacgt aatgccttag ataccagtac aatttcaggt tcaagctgtg atgggaatct
     8281 ttgccaaact tgggataatc cacaaacagc agccgatttt ttaactaaga ttattggtga
     8341 acaatctatt tcgacttgta aagaggattg tggcactaaa accagctcaa aagccggtgt
     8401 aggcttgagt ccggaaattg aggaagaaca tgttcgtact attgtcaaat tagagaatgt
     8461 actgaatatg gatgtgccta aggctgaatt gttagctgat ttgagttcaa ctactgtacc
     8521 agtaacaaga gggcttattg agagtcttcg agatgatcct gatgttgagg tattaagcca
     8581 acgcttatct gcagaaattg cgactagtaa gataatggaa aaaatgctcc tggctcggcg
     8641 agcagtctta gctggtatgc gtgagccaaa tgtcgctcaa aatgcggatg cacaagtaga
     8701 actggaaaga gtactcaata ttattgaccg tgaaatttcc caagtaaaac tagaaatgga
     8761 tatgcaaaaa atgatcaccg gcaatacggc ttcggtgatt ttgcaaagca aatccgaacg
     8821 agaagctctt gctggtgctc atagtttatc tcaagacagt ctggataaac gtgttcaagg
     8881 acttgaaaac ggtaatgttg cattaactga tttagaggca ggctctaata ttaacctctc
     8941 tcgtagaaat attgcattga gtatcccgaa tattccgaat gtagctgctc tcatacctaa
     9001 tctgccaaat cgaggtggtt caggtgtagg tggaaatttc ccatcactcg cacaaatcag
     9061 cggttcaacg atggatcaag caacaggatt actacgtaat tttgaaggct ttatctctaa
     9121 ggctcaatgg gatgtaaatg ctcatcgagt aggatatggt tccgatacaa ttacccgagc
     9181 tgatggtacg atcgtaaaag taaccaaaga tacggtcatt accaaagaag atgcagagcg
     9241 tgatttggcc cgccgaacag ctatcttcgc aaataatgtg agaaaagagc ttggagattc
     9301 aaattggaat gcgttaccgc cgaacgctca agctgtttta gtgtcttatg catataatta
     9361 tggttcactt gctaaaacaa aaagcgtact tgatgcagcc agaagatcag ctcaaagtgg
     9421 tgatatgaat gctttagcta ctgcaatcag aaatcgccaa gttgataata atggcatcaa
     9481 tgcacgtcgc cggaatcagg aagctgacta tattcttggt aaaaattaac ctcataagga
     9541 gttacattta tgcgtaaatt attaggtatt actgtactta cactttgtac aacttcagca
     9601 tttgcaacgg ataaaaatgt ggtattttct tgtaccagca cagaaggtaa acctctcaca
     9661 gttaaacggg taggaaatga ttacgagtat tcgtatgata aaactatctt caaaaaccca
     9721 atcaaaaaag cggtgaccaa cgatggctca attatcgcta gaggatcagg ttttaccacg
     9781 tatgcattag aactgaaaaa tgatggtttg aagtatgtag ttgggtttgt tcaaccaaat
     9841 ggtaactcaa aagagtttat tgagcctgga gcaacgatta gccgtgataa tgatcaacca
     9901 agcattggct ctgtagattg tgatccttac aaaaaaatct attataagtt tgatgttcat
     9961 ttaatgaata ccttgtaatg cgataattca ataatatggg ggaatggctt gatttttcat
    10021 cccccatact ctattatata gatgtggtgc tagagccagc ccacgttaaa aaatacaaag
    10081 ctcggtagat gaactattgt ggtgccagag ccggcccacg ttaaaaaata caaagctcgg
    10141 tagatgaggc cccttaagtc gtaagattta aggggttttg cttaggatat aaaatggata
    10201 ttacaacaca aaatattgaa cttagatact tatctgagca gttttacctt gatgtcgctc
    10261 cacatctgaa agaaattttg cttaaagaga ctcgcccata ctgtgttcta ttagttagag
    10321 ttaaaaattt agattttgcc ctaccattgc gttctaattt acctgaaaga gaaggagttg
    10381 gaattaaaac gatacctaat cctcaggaat acggcaaatt taaaggaatt gatttctcaa
    10441 aagccattct actgacagat aaaagctatt taaaaacaga taccgttcag ctcaaaaaca
    10501 aatcagaatt gttgaatatc caaggtaatg agcgtagaat tgttcgtgaa tttaagaaat
    10561 atgtgagtga a
//
LOCUS       DANLUQ010000081        10536 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.081, whole genome shotgun sequence.
ACCESSION   DANLUQ010000081 DANLUQ010000000
VERSION     DANLUQ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10536
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.081"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            72..1580
                     /locus_tag="PXB27_001583"
     CDS             72..1580
                     /locus_tag="PXB27_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HDL5346354.1"
                     /translation="MSKCPVTHLTMNNGAPVVDNQNSLTAGPRGPLLAQDLWLNEKLA
                     NFVREVIPERRMHAKGSGAFGTFTVTNDITKYTRAAIFSQVGKKTEMFARFTTVAGER
                     GAADAERDIRGFALKFYTEEGNWDMVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNMRS
                     ATNNWDFWTLLPEAFHQVTIVMSERGIPASYRHMHGYGSHTYSFINAANERFWVKFHF
                     HTQQGIKNLTNEEAAAIIANDRESHQRDLYEAIERGDFPKWKMFVQIMPETDADKVPY
                     HPFDLTKVWPKSDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGISVSPDRMLQ
                     ARLFNYADAQRYRLGVNHHQIPVNAPRCPVHSNARDGQGRVDGNYGSTIHYEPNSFGK
                     WQEQAQYAEPPLKINGDAAHWNYREDDADYFSQPRVLFNMMNDEQKQALFNNTAAGMG
                     DALDFIKYRHIRNCYACDPAYGEGVAKALGMTVEDAIAARATDPALGQPGLL"
     gene            complement(1645..1866)
                     /locus_tag="PXB27_001584"
     CDS             complement(1645..1866)
                     /locus_tag="PXB27_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HDL5346355.1"
                     /translation="MNVRLPASGLVCPFPLVEAKAAMAKLNKGDSLTIEFDCTQATEA
                     IPNWAEEEGYEVTDYQQLGDALWEITVVK"
     gene            complement(1895..2404)
                     /locus_tag="PXB27_001585"
     CDS             complement(1895..2404)
                     /locus_tag="PXB27_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HDL5346356.1"
                     /translation="MLFTGLLCGFLLGFVMQRGRFCITGAFRDLYVTKNSRMFVALLI
                     AITVQSIGIWLLYEAGSFSSPAEDLPLLAVIIGAFLFGIGIIYAGGCATGTWYRAGEG
                     LIGSWVALVIYGLFSASMRTGVLAPLNQELKSNVIQHRTIYETFGISPWVLVFILSVI
                     TLALSAHSI"
     gene            complement(2511..3845)
                     /locus_tag="PXB27_001586"
     CDS             complement(2511..3845)
                     /locus_tag="PXB27_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDL5346357.1"
                     /translation="MQLYSYICLLFGLAMFISFFTRKLNENIQTTIAITASALVGSLL
                     ILTFGSLGWFNVDKLAISIFEQLDFKSFLLNGMLGFLLFAGSLGIKLPLMKEQRREIA
                     VYALFSTLVSTFLIGALIYGVARLLGLELGFVYCLLFGSLISPTDPIAVLAIIKSLKA
                     PKRLSMHVEGESLFNDGIGLVIFTTIFAVAFGGQEPNLSGITGLFLQEAVGGIVFGLL
                     TGFVAHKFISATDDGSLEILITLTIPTVGFVIANYLHVSGALTMVVAGIMIGNWTRRS
                     GFSEQSQKYLDHFWEMIDHSLNYLLFLLIGLSTLLVDFTFIGGVLMLAAIPICLLARY
                     ISLWIPYQVLKRFRTYNPYTLKIMTWGGLRGGLALAMALSVPAGAAVVSSSEGNLDVR
                     DLILLMTYAVVTFSILVQGSTVEPMIKKSKLVDPRKVALNKLGAPDDVIPHI"
     gene            complement(3956..5104)
                     /gene="dapE"
                     /locus_tag="PXB27_001587"
     CDS             complement(3956..5104)
                     /gene="dapE"
                     /locus_tag="PXB27_001587"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HDL5346358.1"
                     /translation="MKNEIISLAQNLIRRDSISPQDKGCQQEIAQRLEKLGFNIEWLP
                     FGDTLNLWATHGSENGKTEPCIVFAGHTDVVPVGDESQWAYPPFSAEIVDGMLYGRGA
                     ADMKGSLSALVVAAETFVQHHPNHKGKVALLITSDEEAAAKDGTVKVVETLMARNEAV
                     HYAVVGEPSSSKVFGDVIKNGRRGSITADLYIEGIQGHVAYPHLAENPVHTSLGFLTE
                     LTTYQWDNGNEFFPPTSLQIANIKAGTGSNNVIPGELYVQFNLRYCTEVTDEIIKAKV
                     AEMLEKHGLKYRISWNLSGKPFLAGNGKLVEAALQAVENVTKITPRLDTGGGTSDGRF
                     IALMGAEVVEFGPLNATIHKVNECVSADDLAHCGEIYYQILEKLLATA"
     gene            complement(5234..5581)
                     /locus_tag="PXB27_001588"
     CDS             complement(5234..5581)
                     /locus_tag="PXB27_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HDL5346359.1"
                     /translation="MTTLVYGIKNCDTVKKALKWLEDHNQNPQLHDYRVDGLNADWLV
                     EMEAKFGWENLVNKRSTTWRNLDEEIKNNLNRESALKVLTEQPTLIKRPIVIKEDIAL
                     IGFNEKEYVAAFN"
     gene            complement(5581..6438)
                     /gene="folP"
                     /locus_tag="PXB27_001589"
     CDS             complement(5581..6438)
                     /gene="folP"
                     /locus_tag="PXB27_001589"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDL5346360.1"
                     /translation="MQIHFKNFQKNDRLLDLTTPQIMGILNFTPDSFSDSGKFFSLDK
                     ALFQAEKMLADGASIIDIGGESTRPNAAIVSLEQELERVVPVVEAIRSRFDCLISVDS
                     SKAEVFKESAKVGMDIINDIRALTEPNALAAAVELGLPVCLMHMQGNPQTMQQNPHYD
                     DVLEDVADFLNQRIFECITAGIPKEHIILDPGFGFGKSVKHNYQLLKHLKAFVDSGFP
                     VLAGLSRKSMIGAVLDKPVEQRLLGSVVGALLAAQNGAKILRVHDVAETADALKIWQA
                     MLESDNEEK"
     gene            6427..6564
                     /locus_tag="PXB27_001590"
     CDS             6427..6564
                     /locus_tag="PXB27_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346361.1"
                     /translation="MDLHIFAYILLVQASTLVPLYVDLQHKRGRLRYLTLSLSYLVIS
                     P"
     gene            complement(6547..7509)
                     /gene="rluC"
                     /locus_tag="PXB27_001591"
     CDS             complement(6547..7509)
                     /gene="rluC"
                     /locus_tag="PXB27_001591"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HDL5346362.1"
                     /translation="MTTEKVISAGVQFFTISEDEAGQRLDNFLLAKLKGVPKSLIYRI
                     VRKGEVRVNKGRNKPEYKLQPDDLVRVPPVRVAEKNEAPISTKLNKVAELESQILYED
                     ESMLVINKPSGIAVHGGSGLSFGVIEALRALRPQARFLELVHRIDRDTSGILLVAKKR
                     SALRSLHEQLREKVVQKDYLALVRGQWQAHTKVIKAPLLKNELASGERIVRVSEEGKP
                     SETRFSIEERYANATLVKASPITGRTHQIRVHTQYAGHPIALDDKYGDAEFDSKMKEV
                     GLQRLFLHAHAIRFEHPKTGEEMVITAPLDKTLKGVLAKLRANY"
     gene            7618..8520
                     /locus_tag="PXB27_001592"
     CDS             7618..8520
                     /locus_tag="PXB27_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HDL5346363.1"
                     /translation="MTEKVTETAPNIDVVAETEKVIHKVSNMNIETMLNEWIIPYGTK
                     ILLAIAIFVVGKFLARLISRLLGKAALKSTKDEMLQSFIVSISYFLLLLIVVIASLSQ
                     IGINTSSLVALIGAAGLAIGLSLQNSLQNFAAGVMLLIFKPFRKGDLIETGGMTGVVE
                     QMGLLVLELRTGDNKTVLLPNGKVFSDSIINYSNNPTRRIDFTFDISYESNLKEAKEI
                     IQRILDANTYVLKDPAPVVAVGALAPHSVQLVVRPWVQTPNYWAAYWEIIEQVKLAFG
                     EAGISIPYNQMELHIKSGSLENKA"
     gene            8618..9115
                     /gene="rraA"
                     /locus_tag="PXB27_001593"
     CDS             8618..9115
                     /gene="rraA"
                     /locus_tag="PXB27_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HDL5346364.1"
                     /translation="MRIDTSALCDLYSDQVDVVEPIFSSFGGASSFFGKITTVKCFES
                     NGLIAEVLEEEGEGRVLLVDGGGAVRRALIDAELAQLAVDNGWEGIIVYGAVRQLDIL
                     ERLDIGIHALAPIPVGADNTDIGEVDTPVNFGGVTFFPEDYVYADLTGIILSQELLDL
                     NDISE"
     gene            complement(9163..10524)
                     /locus_tag="PXB27_001594"
     CDS             complement(9163..10524)
                     /locus_tag="PXB27_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDL5346365.1"
                     /translation="MLFLNKIKQLFIFAFTLVLSACSLVVYKPVETIKQVNVERGYRQ
                     KNVIAESAKDGNIVVIMFSGGGTRAAALGYGVLEEFKKVRVKETNKGNTLLENIDIAF
                     GVSGGSILATYFALEGKDIIPKFADRFLKANFQKDIITEVFSLSNLPRLSSPQYGRGD
                     LLQERLNFALYHGKKFGDLVKNRKGPFAVISATDMNIGQKLTFTQETFDGLCLNLNDL
                     EISRAVAASSSVPLIFAPLTLNNNGGNCHFSMPQEFSAREKAIDGLITKNIEETNHIL
                     SLYQDSKERPFIHLVDGGLTDNLGLASVLEISDVMGIEEMYQYIHQLRVKNIVVINVN
                     AQNEVSSEIDKTANVPGVADVVNTIVNVPIDRTTQINLQRYRQFTDGWNEYANKQPSK
                     IEMHFVSLGLKELPDGDLKKDVLNIATSFYLPEEDVDKLREAARVLLQQSQEYNAALK
                     TLQ"
BASE COUNT         3088 a         2363 c         2047 g         3038 t
ORIGIN      
        1 aaaatctaca acacctataa ttctcttcgg attaattacc tatcttattt ctaatttatg
       61 aggattctat tatgtctaaa tgtcctgtta cacatttgac aatgaacaac ggtgcacctg
      121 ttgttgataa ccaaaacagc ttaacagcgg gtccacgtgg tccattatta gcgcaagatt
      181 tatggttaaa tgaaaaatta gcgaacttcg tgcgtgaagt cattcctgag cgtcgtatgc
      241 acgctaaagg ttccggtgcg ttcggtactt tcactgtaac taacgacatc actaaataca
      301 ctcgtgcggc aatttttagc caagtcggta aaaaaactga aatgtttgct cgtttcacca
      361 ccgttgcagg tgaacgtggt gcggcagatg ctgagcgtga tattcgtggt ttcgcattaa
      421 aattctatac cgaagaaggt aactgggata tggtgggtaa caacacccct gtgttcttct
      481 tacgtgaccc acgtaaattc ccggatttaa acaaagcggt taaacgtgac ccacgcacga
      541 atatgcgttc tgcaactaac aactgggatt tctggacatt attaccagaa gcatttcacc
      601 aagtgactat tgtaatgagt gaacgtggta ttccggcttc atatcgtcat atgcacggtt
      661 atggctcaca tacttatagc ttcatcaatg cagctaatga gcgtttctgg gtgaaattcc
      721 atttccatac acaacaaggc attaaaaact taactaacga agaagcagcc gctattatcg
      781 ctaacgatcg tgaaagtcac caacgtgatt tatacgaagc gattgagcgt ggcgatttcc
      841 cgaaatggaa aatgtttgtt caaattatgc ctgaaactga tgcagacaaa gtaccatacc
      901 acccgttcga tttaactaag gtatggccga aatcagacta tccgttaatt gaagtgggtg
      961 agtttgaatt aaatcgtaac cctgaaaact tctttgctga tgtggaacaa tctgcattcg
     1021 caccaagtaa cttagtgccg ggcatcagcg tgtctccaga ccgtatgtta caagctcgtt
     1081 tattcaacta tgcggatgct caacgctacc gtttaggtgt gaaccaccac caaatccctg
     1141 taaacgctcc acgttgcccg gttcatagca acgctcgtga cggtcaaggt cgtgtggacg
     1201 gtaactacgg tagtaccatt cactacgaac caaacagctt cggcaaatgg caagaacaag
     1261 ctcaatatgc agagccacca ctgaaaatta atggtgatgc agcccactgg aactatcgtg
     1321 aagacgatgc tgactacttc agccaaccac gtgtattgtt caatatgatg aatgatgaac
     1381 aaaaacaagc attattcaat aatactgctg caggtatggg cgatgcatta gacttcatta
     1441 aataccgtca tattcgtaac tgctatgcct gtgatccggc atatggtgaa ggtgtggcaa
     1501 aagcattagg tatgacggtt gaagatgcta ttgctgcaag agcaacagac ccagcattag
     1561 gtcaaccggg tttactctaa tccttagtaa atacaaaact caaagcaaaa gctctacaaa
     1621 aaagtagagc ttttttattg ctaattactt aaccaccgta atttcccaca aggcatcacc
     1681 aagttgttgg taatcggtaa cttcatagcc ttcctcttcc gcccaatttg gaatggcttc
     1741 ggttgcttga gtacagtcaa attcaatcgt taagctatcg cctttattca attttgccat
     1801 cgccgctttg gcttcgacta atggaaacgg gcaaacaagc cctgaggcag gtaaacgtac
     1861 gttcatcttt attactcctt aaaaaattaa ccttttaaat gctgtgggcg gataatgcca
     1921 atgtaattac actcaggata aacactaaca cccaagggga aataccaaat gtttcataaa
     1981 tcgtgcggtg ttgaattaca ttgcttttta gctcttgatt aagtggggct aacacaccgg
     2041 ttcgcataga agcagaaaac agcccataaa taaccagtgc aacccaactg ccgattaaac
     2101 cctcaccggc acgataccac gttcctgtag cacaaccgcc tgcataaata atgccgatac
     2161 caaacagaaa tgccccaatg atcactgcga gcaaaggcaa atcttccgcc ggagaactaa
     2221 aagagccggc ttcatacaaa agccaaatcc cgatcgattg caccgtaatt gcaattaaaa
     2281 gtgccacaaa catacggcta ttctttgtta catagagatc acgaaatgct ccggtaatac
     2341 aaaaacgccc tctttgcatc acaaacccga gtagaaagcc acataataat cctgtgaata
     2401 acataaaaaa tacctctcaa caaattaacg tgagaggtat ttaaatataa agccgtctag
     2461 atggctaata atctttagca aaatgatatg ttattcagtt ataaatgcta ttaaatatgt
     2521 ggaattacat catcaggtgc acctaattta ttgagtgcca cttttcttgg atctaccaac
     2581 ttggattttt ttatcatcgg ttcaaccgtt gaaccttgta ctaaaatcga gaaagtgact
     2641 accgcataag tcattaacaa aatcaaatca cgtacatcaa gattgccttc tgagctcgat
     2701 accaccgcag cccctgccgg tacggaaagt gccatcgcaa gggctaatcc accacgtaaa
     2761 ccgccccaag tcataatttt taaggtgtac gggttataag tacggaaacg ttttaacact
     2821 tggtatggaa tccacaggct gatatagcgt gcaagcaaac aaattggaat tgctgccaac
     2881 attaacaccc caccaataaa ggtgaaatca actaacagtg tacttaagcc gataagcagg
     2941 aataacaggt agtttaatga atggtcgatc atctcccaga agtgatcaag atatttttgg
     3001 ctctgttcgg agaaaccgga acggcgagtc cagttgccaa tcataatacc ggcaactacc
     3061 attgttaatg cccctgaaac gtgtaggtag tttgcaatca caaagccgac tgttggaatc
     3121 gttagcgtaa ttaaaatttc aaggcttccg tcatcggttg cggaaataaa tttatgtgcc
     3181 acaaaaccgg ttaataagcc aaaaacgata ccgcctaccg cttcttgcaa gaacagtccg
     3241 gtaataccgc taaggtttgg ttcttgtccg ccaaaagcaa cggcaaagat agtggtaaaa
     3301 atcaccaagc cgataccgtc attaaacaga gattcgccct ccacgtgcat cgaaaggcgt
     3361 tttggggctt tcaggctttt gataattgcc aatacggcaa tcgggtcggt tggcgaaatc
     3421 agtgagccaa acagcaaaca atacacaaag ccgagctcta acccaagtag ccttgccacg
     3481 ccataaatta acgcaccgat caagaaagta gaaactaagg ttgaaaataa ggcataaact
     3541 gcaatttccc gccgttgctc tttcattaat ggtagtttaa tcccaagtga accggcaaaa
     3601 agcaagaacc ccagcattcc gtttaacagg aagcttttaa aatcgagttg ttcaaaaatg
     3661 gaaatggcaa gtttatcaac attaaaccag ccgagagaac cgaaggtaag aataagaagg
     3721 gagccgacaa gggcagaggc tgtaattgca atagtggttt gaatattttc gtttaatttt
     3781 cgggtaaaaa acgaaataaa catcgcaagc ccaaacagca agcagatata agagtagagt
     3841 tgcataacat ttacctataa atataaaaac tgccgctagt gtaaacgctg tttaacagaa
     3901 tagctacata aaaaataggc agaatacaat ttgataacat tctgcctatt tagggttatg
     3961 cagttgccaa taatttttct aaaatttgat aataaatttc gccacaatgt gccaaatcat
     4021 ccgcactcac acactcattc actttgtgaa tagtggcgtt cagtggtcca aattccacta
     4081 cctctgcccc cattaatgca ataaaacgac cgtccgaagt accgccacct gtgtctaaac
     4141 gaggcgtaat ttttgtgaca ttttcgaccg cttgtaaggc ggcttcgacc aattttccat
     4201 tgccggcaag gaatggtttg cctgataaat tccaacttat tcggtatttc aaaccgtgtt
     4261 tttcgagcat ttccgctact ttggctttga tgatttcatc ggtcacttca gtgcaataac
     4321 gtaagttaaa ctgcacataa agctcgcccg gaatcacatt attactgcct gtgccggctt
     4381 tgatattggc aatttgcaaa ctggtaggcg ggaaaaattc gttaccgtta tcccactgat
     4441 aagtggtcag ctcggttaaa aaacccagtg acgtatgcac cggattttcc gccaaatgtg
     4501 gataggcaac gtgcccttga atgccctcga tatagagatc tgccgtaatt gaaccacgtc
     4561 tgccgttttt aatcacatcg ccaaacacct tgctactaga cggttcgccc actaccgcat
     4621 aatgtaccgc ttcattgcga gccattaagg tttcaaccac ttttaccgta ccgtctttgg
     4681 ctgctgcctc ttcatcggag gtaatcaata atgccacttt gcccttatgg tttgggtgat
     4741 gttgcacgaa agtttctgcc gccacaacta acgccgagag cgagcctttc atatctgccg
     4801 cccctcgccc ataaagcatt ccatctacaa tctcagccga aaacggcgga taagcccatt
     4861 ggctttcatc gcctaccggc accacatctg tatgcccggc aaacacaatg caaggctctg
     4921 ttttaccatt ttccgagccg tgcgttgccc ataaattcaa ggtgtcgcca aacggcaacc
     4981 attcaatatt aaagcccagt ttttcaagac gttgtgcaat ttcttgctga cagcctttgt
     5041 cttgtgggct gatggaatca cggcggatta ggttctgtgc aagggaaata atttcatttt
     5101 tcataggtta caagcggttg ttttgggtga attttttgct atatatcctg taggggcggg
     5161 tcctgtgccc gcccgaacaa tcctaaagac tacggacagg cacaggacct gtccctacaa
     5221 gcaggggaaa aaattaatta aacgcagcca catactcttt ctcattaaaa ccaatcaaag
     5281 caatatcctc tttaatcaca atcgggcgtt taattagggt cggctgttcg gttagcactt
     5341 ttaatgccga ctcacggtta aggttgtttt taatttcctc atcaaggttt cgccaagtgg
     5401 tgctacgttt attaactaag ttttcccaac cgaattttgc ttccatttcc actaaccaat
     5461 cggcatttaa accgtccaca cggtaatcgt gcagttgtgg gttttgatta tgatcttcga
     5521 gccatttcaa ggcttttttt actgtgtcgc aatttttaat gccataaact aaggttgtca
     5581 tcatttttcc tcattatctg attccagcat tgcctgccag atttttaacg catcggcggt
     5641 ttctgccaca tcgtgcaccc gtaaaatttt cgccccattt tgagcggcta ataacgcccc
     5701 tactacagag ccaagtagcc gttgttctac cggtttatct aacaccgcac caatcattga
     5761 tttacgggaa agtccggcaa gcacagggaa gcctgaatcc acaaaggctt taaggtgttt
     5821 caataattgg taattgtgtt tcacgctttt accaaagcca aaaccggggt ctaaaataat
     5881 gtgttctttg ggaatgcctg cggtgatgca ctcgaaaatc cgttgattta agaaatctgc
     5941 cacatcttcc aacacatcat catagtgcgg attttgttgc attgtttgcg ggttgccttg
     6001 catatgcatt aaacaaaccg gcaagcccaa ttctactgcc gctgccaacg cattcggctc
     6061 ggttaaggct cgaatatcgt taatgatatc cataccgact tttgccgact ctttgaatac
     6121 ttctgctttg gagctatcaa ccgaaatcaa gcaatcaaaa cggctgcgga tcgcctccac
     6181 caccggcaca acacgctcta attcttgttc taagctcaca atcgccgcat tagggcgggt
     6241 ggattcaccg ccaatatcaa taatgcttgc cccatcagct aacatttttt ccgcttggaa
     6301 caaggcttta tcgagggaaa aaaacttgcc cgaatcggaa aatgaatccg gcgtgaagtt
     6361 taagatcccc ataatttgcg gtgtggttaa atctaacaag cggtcatttt tttgaaaatt
     6421 tttgaaatgg atttgcatat ttttgcttat attttgttgg tgcaagcgtc cacgcttgta
     6481 ccactctatg ttgatttaca gcacaagcgt ggacgcttgc gctatcttac tctctcatta
     6541 agttatttag taattagccc ttaattttgc caatacgcct tttaaagttt tatccagcgg
     6601 ggcggtaatg accatttctt cgccggtttt ggggtgttca aaacgaatag catgggcgtg
     6661 cagaaataaa cgctgtaagc ccacttcttt cattttgctg tcaaattccg catcaccata
     6721 tttgtcatct aacgcaatcg ggtgtccggc atattgggta tgcacacgga tttggtgggt
     6781 tcttccggta ataggcgaag ctttcaccaa agtggcattg gcataacgct cttcaatgct
     6841 gaaacgggtt tcggacggtt taccctcttc actcacccgt acaattcgct caccgctggc
     6901 aagttcgttt tttagcaatg gagctttaat cactttggta tgagcctgcc attgccctcg
     6961 cacaagggca agatagtctt tttgtactac tttttcacgc agttgttcgt gcaaggaacg
     7021 taacgccgaa cgctttttcg ccaccagcaa aatgcctgat gtatcacggt ctatacggtg
     7081 taccaattcc aagaaacgag cctgtggacg taatgcacgc aaggcttcga tcacaccaaa
     7141 acttaaacca ctgccaccgt gtactgcaat gcctgaaggt ttgttaatta ctagcattga
     7201 ctcatcttca tacagaattt ggctctccag ctccgccact ttgtttaatt tagtcgaaat
     7261 tggggcttca tttttttccg ccacacgcac cggcggcaca cgcactaaat catcaggctg
     7321 taatttatat tcgggtttgt ttcgtccttt attcacccgc acctcgcctt ttcgcacaat
     7381 gcggtaaatc aaacttttcg gcacgccttt gagctttgcc aataaaaaat tatctaaacg
     7441 ctgtccggct tcatcttcac tgatcgtaaa aaattgaacg ccggcactaa ttactttttc
     7501 tgttgtcatt atcttgtctt ttagagaaaa ttttgctcta aattctagca caaaaccgct
     7561 tagcgagtga ggcaaattcg ctaggcttat ttttcatttt aattttaagg aaacaatatg
     7621 actgaaaaag tcaccgaaac agctccaaac attgacgtgg ttgccgaaac cgaaaaagta
     7681 atccacaaag tcagtaatat gaatattgaa actatgctaa atgagtggat tatcccttat
     7741 ggcaccaaaa ttttactggc gattgcgatt tttgtagtcg ggaaatttct tgcccgccta
     7801 attagccgtt tactcggtaa agccgcatta aaatcaacca aagatgaaat gctgcaaagt
     7861 tttattgtct caatcagcta ttttttactg ctgttaattg tggtgattgc ctcgctttca
     7921 caaatcggca ttaacaccag ctcgctggtc gcattaatcg gtgctgccgg tttagccatc
     7981 ggtttatcat tacaaaactc attacaaaac tttgctgccg gcgtgatgtt gttaatcttc
     8041 aagccatttc gcaaagggga tttaatcgaa accggcggaa tgaccggcgt ggttgaacaa
     8101 atggggcttt tagtgttgga acttcgcacc ggcgataaca aaaccgtgtt attaccaaac
     8161 ggcaaagtgt tttctgacag catcatcaac tactctaaca acccgacacg ccgtattgat
     8221 ttcacctttg atatttctta tgaatctaac ttaaaagagg cgaaagagat tatccaacgc
     8281 attttagacg ccaatactta tgtattaaaa gatccggcac ctgtagtagc agtcggcgct
     8341 ttagcaccgc atagtgttca acttgttgtt cgcccttggg ttcagacacc aaattactgg
     8401 gcagcatatt gggaaattat cgaacaagta aaattagcct ttggtgaagc aggtatatct
     8461 attccttaca accaaatgga attgcatatt aaaagcggtt cactagaaaa taaagcataa
     8521 gaaaaaagcc tgtaagcggt cttttttcga taaaattttg caaattttgg gcgaaggttt
     8581 atactttagc taaataatct acgaacagga gcatattatg cgtattgata cctctgcact
     8641 ttgcgatctt tattctgacc aagttgatgt ggttgaacca attttctcaa gctttggcgg
     8701 tgcttcttcc ttctttggga agattaccac ggttaaatgc tttgaaagca acggtttaat
     8761 tgctgaagtt ttggaagaag agggtgaagg gcgagtgctg ttagtcgatg gtggcggtgc
     8821 ggttcgtcgt gccttaattg acgcagagct ggctcaactt gctgtagata acggctggga
     8881 aggtatcatc gtttatggtg cggttcgcca attagatatt ttggaaagat tagatatcgg
     8941 tattcatgct ttagcgccaa ttccagtggg agcagataat accgacattg gcgaagtgga
     9001 tacgcctgtg aattttggcg gtgtaacctt cttccccgaa gattacgttt atgctgactt
     9061 aaccggcatt attctttcgc aagagttgtt ggatttaaac gacatcagcg aataaatatt
     9121 tgcaaaaaaa taccaaaaac cgaccgcttg gtcggttttt tactactgca gtgtttttaa
     9181 tgccgcatta tattcttgag attgctgcaa taaaacccga gcagcttcac gtaatttatc
     9241 tacatcctct tccggcaaat aaaatgatgt tgcaatattt agcacatctt tttttaaatc
     9301 accatcaggt aattctttta aacctaaact gacaaaatgc atttcgatct ttgatggctg
     9361 tttatttgca tattcattcc aaccatctgt aaactggcga taacgttgta aattaatttg
     9421 tgtcgttcta tcaattggca cattaactat tgtattcact acatcagcca cccccggtac
     9481 atttgcggtc ttatcgatct cgctagatac ttcattttgc gcatttacat taatcactac
     9541 aatatttttc accctaagct gatgaatata ctggtacatc tcttcaatac ccataacatc
     9601 agatatttcc agcacactgg caagccctaa attatctgtt aatccaccat caactaaatg
     9661 gataaagggg cgttctttac tatcttgata aaggctgagt atatgattcg tttcttcaat
     9721 attttttgta attagtccat caattgcttt ttctctagca ctaaattctt gtggcatact
     9781 aaaatggcaa tttccaccat tgttattcag tgtaagcggc gcaaaaatca gaggaacaga
     9841 actagaagca gcaacagcac gggaaatttc taaatcattc aaatttaaac aaagcccatc
     9901 aaaggtttct tgcgtaaagg tcagtttttg accaatgttc atgtctgttg cgctaatcac
     9961 agcaaaaggt cctttacgat ttttcactaa atcaccaaac tttttaccat gataaagggc
    10021 aaaatttaag cgttcttgca ataaatcacc acgcccatat tgaggagaac ttaaacgagg
    10081 aagattggat aacgaaaaca cctctgtaat aatatctttt tgaaagttcg cttttaaaaa
    10141 acggtcagca aatttaggaa tgatatcctt tccttccaac gcaaaataag tggctaatat
    10201 cgatccacct gatacgccaa aggcaatatc tatattctct aataaagtat tacctttatt
    10261 tgtttctttc acccttacct ttttaaactc ttccaacact ccataaccaa gcgccgccgc
    10321 tcttgttcca ccacctgaaa acatgatgac aacaatatta ccgtcttttg cactttcagc
    10381 gatcacattt ttttgtcgat aacctctttc aacatttacc tgtttaattg tttcaactgg
    10441 cttataaaca accaaactgc aagcagataa cactagcgta aaggcgaaga tgaaaagctg
    10501 ttttattttg tttagaaaaa gcataaaaac cctaaa
//
LOCUS       DANLUQ010000082        10466 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.082, whole genome shotgun sequence.
ACCESSION   DANLUQ010000082 DANLUQ010000000
VERSION     DANLUQ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10466
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.082"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            2..646
                     /gene="sspA"
                     /locus_tag="PXB27_001595"
     CDS             2..646
                     /gene="sspA"
                     /locus_tag="PXB27_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HDL5346366.1"
                     /translation="MTSSINKRSVMTLFSDKNDIYSHQVRIVLAEKGVPYEIENVMLG
                     SISEDLLELNPYGNIPTLVDRELVLFNPRIIMEYLDERFPHPPLMPVYPVSRGQCRLN
                     MYRIEQDWYSQIDIVNKDPESAEGKKALAQLKEDVLALAPIFAAKPYFMSDDFSLVDC
                     YIAPLLWRMQILGVKFSGATAKPINAYMTRVFQRDSFIQSVGGEAPKHLMDDKD"
     gene            646..1053
                     /locus_tag="PXB27_001596"
     CDS             646..1053
                     /locus_tag="PXB27_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HDL5346367.1"
                     /translation="MKPLRPYLYNAYYNWIIDSDNTPYLLVNAEFPDVDVPVEFVKEG
                     KIILNISPRSIGQYIVADEYISFSARFQGMLRDIYIPFGAMVAIYAQETGDGIMFQEE
                     AYYTEEAYQARVRAVEQPKKIKAKKTSTLKLVK"
     gene            1250..2209
                     /locus_tag="PXB27_001597"
     CDS             1250..2209
                     /locus_tag="PXB27_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5346368.1"
                     /translation="MKFHKTLLVTLLSSLSVAALAQDKIQNVAITAIVEHPALDSIRK
                     GVIEELAREGFVDGKNIKIDYQSAQGSTATAAQIARKFVGDKADIIIPITTPSAQPVV
                     AATRSIPIVFSGVTDPVAAKLVKSWEPSGTNVTGISDHKAMAPQVKLIQTLVPDLKAV
                     GYVYSAGEVNSAIVLEELKQEAQKQGFTIVPVAVQRSADIGTAARSLNGKVQAIYISE
                     DNAVVSAYEALHKAALEAKIPVIAADRDTVQRGAVAAYAVNQYDIGVATGKVAARVLK
                     GEKAGTIPIQEVSKLELSINTKTAKELGITIPEVLIKEAKQTF"
     gene            2289..2549
                     /locus_tag="PXB27_001598"
     CDS             2289..2549
                     /locus_tag="PXB27_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005718542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL5346369.1"
                     /translation="MGWIAAIIVGAIIGWIASAVMKSEGGLCKNIIIGILGSSLGRWV
                     FGDVLGIGSAYSAGDFNIVGIFFGVLGAALLIFILRKLSIIN"
     gene            complement(2599..3489)
                     /locus_tag="PXB27_001599"
     CDS             complement(2599..3489)
                     /locus_tag="PXB27_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL5346370.1"
                     /translation="MDKLNAISLFCKVIETQSFTQAAQQAQISLPMASKLVAQLEEHL
                     NVRLLQRTTRKITPTEAGLLYYQQCLPILNELKEADASVSNINSTLQGKITMSVPMDF
                     GSRFIAPYLGKFMEDYPNLALHIEFSDRRVDVIAEGFDLVLRIGSLADSSIVAKKIAD
                     SHLVLVASPDYLARFGEPKGLEALVERDCLLYEYHPQWQFSQQGQKMQIKPQGKIYSN
                     NGYALVQMAKAGLGIINIPKFLIKHELKNGELIPIMPFLTQEPLQISLLYPNRRYLSP
                     KVRILIDFFTQLMQEQKEYI"
     gene            3619..6225
                     /gene="topA"
                     /locus_tag="PXB27_001600"
     CDS             3619..6225
                     /gene="topA"
                     /locus_tag="PXB27_001600"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDL5346371.1"
                     /translation="MGKSLVIVESPAKAKKINQYLGSNYVVKSSVGHIRDLPKSGSDE
                     KTEKAKPISTKGLTAAEKAKIKAEKERVALVKRMGIDPYNGWKANYQILPDKEKVVSE
                     LKSLAKKADHIYLATDLDREGEAIAWHLREIIGGDDSRFSRVVFNEITKDAIKKAFDD
                     PQPLNIDRVNSQQTRRFLDRVVGFMVSPLLWKKVARGLSAGRVQSVAVKLLVEREREI
                     KAFQSEEFWTILANTQINRKKLPLELTTYKGKKFSASNEAEANVAVRALEQSDFIVSN
                     IEAKETSSRAAAPFITSSLQRAASTRLGFGVKKTMMLAQRLYEAGYITYMRTDSTNLS
                     REALAMARGYIQDNFGANYLPEKANVYRSKDNAQEAHEAIRPSDVNVKMADLKGMDKD
                     AERLYDLIWRRFLACQMLPARYDSTAVTVSAGDYELKANGRVLRFDGWTKVLPVQGKN
                     PEDQELPAVELHQKLTLDEIVPSQHFTKPPARFNEASLVAELEERKIGRPSTYASIIS
                     TIQERGYVRTENKRFYVEKIGEVVTDRLNESFKDLMNYDFTANMEEVLDQIAEGSKNW
                     KEELNTFFKHFSEELSQAELDELEGGMKPNSLVPTDIDCPTCGRKMAIRTASTGVFLG
                     CTGYALPPKERCKTTMNLIPEAEFFNVLDSDSETKALMARKRCPKCDSAMDSYVIDAE
                     RKLHVCGNNPNCDGHIIEQGSFKIKGYDGPVVECDKCGSDMHLKLGRFGKYMGCTSCD
                     NTRKILKNGEVAPPKEDPIAFPELKCEKADAYFVLRDGASGVFMSAHNFPKVRESRAP
                     KVVELAQYRERLPEKLQYLADAPQQDPEGNEAIIRFSRKEKRQYVTSEKNGKATKWIV
                     DYLDGKWVERKK"
     gene            complement(6275..7213)
                     /gene="yvcK"
                     /locus_tag="PXB27_001601"
     CDS             complement(6275..7213)
                     /gene="yvcK"
                     /locus_tag="PXB27_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HDL5346372.1"
                     /translation="MENTDIIPFKPTRHHYLNQLNAVVALGGGHGLGRLLSALSFLKE
                     RLTGIVATTDNGGSTGRIRSQQGVIAWGDLRNCLNQIIVKPTIASRLFEYRFAGTGEL
                     AGHNLGNLILTALENMQIRPLEGINLVRELLHVRSQLIPMSEIPVHLIAQLHSGESVF
                     GEVQIDSLAETPQHLALDTMVVATPEAISAIQSAELILLGPGSFFTSIMPNLLVAGIA
                     NSIAQSKARVVFIDNIGQEHGPAGQLSLNQRIEWMENCIGQARINAVITTDDAGSELP
                     EHIQILRKNLSANDVYYRHDRNKLSKAIDELLAQNI"
     gene            complement(7227..8303)
                     /locus_tag="PXB27_001602"
     CDS             complement(7227..8303)
                     /locus_tag="PXB27_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL5346373.1"
                     /translation="MFQMLQDWYRKKFTDPQIVVLFSILLIGFGIIYFFSDLLMPLLV
                     ALVFAYLLEWPIRFLSSKLKLPRTLSVILVLGGFIALLSFLGVVLLPSLWNQAVTFIQ
                     DLPSMFNLLNAWLQALPEHYPELVDYATLDSIVNTAKSNILSMGESLLTLSINSIISL
                     VGLGIYTFLVPLMVFFLLKDKIVLMRSFSKVLPQNRRLATRVWFEMQQQIANYIRGKF
                     LEILIVGVVTYIIFLFFDLRYPLLLSVAVGISVLVPYIGAVLVSIPVMLIALFQFGLS
                     PDFYYLMLAFIISQLLDGNLLVPFLFSEAVNLHPLTIIIAVLIFGGLWGFWGVFFAIP
                     LATLVKAVLQALPSAQSDAIILEK"
     gene            complement(8334..9089)
                     /locus_tag="PXB27_001603"
     CDS             complement(8334..9089)
                     /locus_tag="PXB27_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL5346374.1"
                     /translation="MKKEEVGHHFLARLGKTRLRPGGKLATDWLIANGDFDKNKKVLE
                     VACNMGTTAIQLAQQFECQIVGIDLDEDALEKARKNIKENHVEHLIQVQRANATKLPF
                     DDESFDIVINEAMLTMLPLEAKEKAIREYFRVLKPNGFLLTHDIVIQDKDSDTLLAEL
                     RDAINITVSPLAKADWKSLFQRCGFRNVDTFSGEMSLLSPKGLIHDEGVLGAMKIIGN
                     ALKPENRETFNKMYKLFNDPDKKLGFIAVCSQK"
     gene            9241..9450
                     /locus_tag="PXB27_001604"
     CDS             9241..9450
                     /locus_tag="PXB27_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome alternative rescue factor ArfA"
                     /protein_id="HDL5346375.1"
                     /translation="MSKQKNDYLHQRGQIKESAVKALVTDPLFRQRVERNRKGKGSYR
                     RQEKHKKTIYGQKETPFKNIRLESF"
     gene            complement(9499..10221)
                     /gene="bioD"
                     /locus_tag="PXB27_001605"
     CDS             complement(9499..10221)
                     /gene="bioD"
                     /locus_tag="PXB27_001605"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HDL5346376.1"
                     /translation="MPSLFITGTDTNVGKTVVTRAIIQTLTKHSFPVIGYKPIACGGD
                     DSLPTEPTQYDYATEDNSDVLTILDSCPTNVSYRDINSYTFIHSSTPVFAALDAVHHI
                     QLEKLNTDLKRLENAYPNVVVEGTYGWLTPINKDYYFADWVMQNNMPVVLVVGIKEGC
                     VNHALLTANEILQRGVKLVSWVANRVNPGLRHYHELIELLTQKINAPLLGQIPYMGHP
                     EHKELSSYIQNPEPLLAYFSKP"
BASE COUNT         3273 a         1860 c         2206 g         3127 t
ORIGIN      
        1 aatgacgagt agtataaaca aacgttcggt aatgactctt ttttcggata aaaatgatat
       61 ttacagccac caagtgcgta ttgtgttagc agaaaaaggg gtgccttatg aaatagagaa
      121 cgttatgcta ggcagtattt ctgaagattt attagaattg aatccttacg gtaatattcc
      181 tacattggtt gatcgtgaat tagtgctgtt taaccctcgt attattatgg aatatttaga
      241 tgagcgtttc cctcacccac cattaatgcc ggtttaccct gtttctcgtg gtcagtgccg
      301 tttaaatatg tatcgtattg aacaagattg gtattcgcaa attgatattg tgaataaaga
      361 tcctgaatca gcggaaggca agaaagcgtt agcacagctt aaagaagatg tattggcatt
      421 agcgcctatt tttgccgcta aaccttattt tatgagcgat gatttcagct tggttgattg
      481 ctatattgca ccgttattat ggcgtatgca aatcttaggc gttaaattca gcggtgcaac
      541 ggcaaaacca attaatgcgt atatgacccg tgtattccaa cgtgatagtt ttatccagtc
      601 tgttggcggc gaagcgccaa aacatttaat ggacgataaa gactaatgaa accattaaga
      661 ccttatcttt ataatgccta ctataactgg attattgata gcgataacac gccttactta
      721 ttagtgaatg cagaatttcc agacgtagat gtgccggtag agtttgtcaa agaagggaag
      781 attattttaa atatttcgcc tcgttccatc gggcagtata ttgtggctga tgaatacatt
      841 agctttagcg ctcgttttca gggaatgtta agagatattt atattccttt tggggcaatg
      901 gtggcgattt atgcgcaaga aaccggtgat ggtattatgt tccaagaaga agcctactac
      961 actgaagagg cttatcaagc tcgagttaga gcagtagagc agcctaaaaa aatcaaggcg
     1021 aagaaaacat ctaccttaaa gttggtgaaa taagagatct atttcatcaa aacacattac
     1081 aagcggttaa atttttctga ttatttgcaa atatcagtca aaatttaacc gcttgtttgt
     1141 tttgtgacgg aaataatcaa attccacttg cactccgact aaaaattgct tatctttgtt
     1201 ttgtgtttgt actgttgtga tagtacagta atgatcttaa ggagagaaaa tgaaattcca
     1261 taaaacctta ttagtgactt tgttaagttc actttctgta gcggcattag ctcaagataa
     1321 aattcaaaat gtagcaatta cagcgattgt ggaacaccct gcattagatt caattcgtaa
     1381 aggtgtgatt gaagaattag cccgagaagg gtttgttgat ggtaaaaaca ttaaaatcga
     1441 ctatcagtct gcacagggta gtactgcaac agcagcacaa attgcgcgta aatttgttgg
     1501 ggataaagca gatattatta tcccgatcac cacaccttcg gcacaacctg ttgttgctgc
     1561 aacacgttct atcccaattg tgttttcagg cgtgacagac ccggttgctg caaaattagt
     1621 aaaatcgtgg gagccaagtg gcactaacgt gacggggatt tcagaccaca aagcgatggc
     1681 tccacaagtg aaattaattc aaacattagt accggatttg aaagctgttg gttatgtgta
     1741 tagtgcaggt gaagttaatt ctgcgattgt gttagaagag ttaaaacaag aggctcaaaa
     1801 acagggcttt actatagtgc cggttgctgt tcaacgcagt gccgatattg gcactgctgc
     1861 tcgcagccta aacggaaaag tgcaggctat ttatatttct gaagataatg cagttgtttc
     1921 ggcttatgaa gccttacata aagcggcttt agaagcaaaa attccggtga ttgctgctga
     1981 ccgtgataca gtacaacgtg gtgcagtggc agcttatgcg gttaatcaat atgatattgg
     2041 tgtggcaacc ggtaaagttg ctgctcgtgt attaaaaggg gaaaaagcag gtacaattcc
     2101 aatccaagaa gtcagcaaat tagagctttc aattaatact aaaacggcaa aagaattggg
     2161 cattactata ccagaggtat taatcaaaga agctaaacaa actttttagt gtaaaaaata
     2221 aaaatttgtt ttatttgtca taatcttgta gagtaaaaat tcatttcaac tcagctaagg
     2281 agaatattat gggatggatt gctgcaatta ttgttggcgc tataattggt tggatagctt
     2341 cggctgtaat gaaaagtgaa ggcggacttt gcaaaaatat tattattggt attttgggtt
     2401 cgtcacttgg acgctgggta tttggtgatg tattaggtat tggttccgct tatagtgccg
     2461 gagactttaa tatagtcggt atcttttttg gtgtattagg ggcagctctt ttgattttca
     2521 ttttgagaaa attaagcata attaactaat ttcgtatttc agaaaggaag cagttgtttt
     2581 ataactgctt cttttttgtt aaatatactc tttttgctct tgcatcagtt gtgtgaaaaa
     2641 atcaattagg attcgcactt taggagagag ataacgacgg tttggataga gtaagctgat
     2701 ttgtaaaggt tcctgtgtca aaaaaggcat aataggaatc aactctccgt tttttagctc
     2761 gtgttttatt aaaaactttg gaatgttaat gatgcccaaa ccagcttttg ccatttgtac
     2821 taacgcataa ccattgttac tgtaaatttt accttgtggt ttaatttgca ttttttgtcc
     2881 ttgttgagaa aattgccatt gaggatggta ttcatagagt aggcagtctc gttccactaa
     2941 tgcttctaag cctttaggtt cgccaaatcg agctaaataa tctggagaag caactaaaac
     3001 caaatgggaa tctgcaattt ttttcgctac aatggaacta tctgcaaggc taccaatacg
     3061 cagcaccaaa tcaaagcctt ctgcaatcac atctacccga cggtcactaa attcaatatg
     3121 aagggctaaa ttagggtaat cttccataaa tttcccaagg taaggggcaa tgaagcgaga
     3181 gccaaaatcc attggtacag acattgttat tttcccttgt agcgttgagt taatattact
     3241 tacgctggca tcggcctctt ttagttcatt taaaataggc aagcattgct gatagtagag
     3301 caaccctgcc tcagttggcg taatttttcg agtggttcgt tgtagtaaac gcacattgag
     3361 atgttcttct aactgagcca ccaatttact tgccatgggt aacgaaattt gagcttgttg
     3421 tgctgcttga gtgaaacttt gggtttcaat tactttacaa aataaactga ttgcgtttag
     3481 tttatccatt ttctctcttc tttatattgt aatttgtgtt aaggcttgct atcgtaaaaa
     3541 tagtgtctaa aaaaatcaac ttaaaaagaa ataatttcaa acaatttaaa ataattatta
     3601 aaaattaggt aataaattat gggaaaatca ctagttattg tggagtctcc ggctaaggca
     3661 aaaaaaatta accaatatct tggtagtaat tatgtggtga aatcgagtgt ggggcatatt
     3721 cgtgatttac cgaaaagtgg tagtgatgaa aaaacggaaa aagcgaaacc gatttcaacc
     3781 aaagggctga ccgctgccga aaaagctaaa atcaaagcag aaaaagagcg tgttgcattg
     3841 gttaaacgta tgggtatcga cccctataat ggctggaaag ctaactatca gattttgccg
     3901 gataaagaga aagtggtttc cgagctaaaa tcgttggcaa agaaagcaga tcatatctat
     3961 ctggcaaccg acttggatag agaaggggag gcgattgcgt ggcatttgcg tgaaatcatt
     4021 ggtggtgatg acagccgttt tagccgagtg gtatttaatg aaatcaccaa agatgcgatc
     4081 aaaaaggctt ttgacgatcc gcaaccgctc aatattgacc gtgtaaattc ccagcaaacc
     4141 cgccgctttt tagaccgtgt agtgggcttt atggtgtcgc cattattatg gaaaaaagta
     4201 gctcgtggtt tatcggcagg gcgtgtgcaa tcggttgccg taaaattact ggttgagcgt
     4261 gagcgtgaaa tcaaggcgtt ccaaagcgag gagttttgga ctattcttgc caatacgcaa
     4321 attaatcgta aaaaattacc gcttgaatta actacttata aaggtaaaaa attctcggca
     4381 agcaatgagg ctgaagcaaa tgttgcagtg cgtgctttgg agcagagcga ttttattgtt
     4441 tctaatattg aagccaaaga aaccagctct cgagctgctg caccatttat tacctcatcg
     4501 ttacaacgtg ccgctagcac tcgtttaggt tttggggtta aaaagacgat gatgttagcc
     4561 caacggttat atgaagcggg ttacatcacc tatatgcgta ctgattcaac caaccttagc
     4621 cgtgaagcat tagcaatggc tcgaggttat attcaggata atttcggggc gaattatttg
     4681 ccggaaaaag caaatgtgta tcgcagtaaa gataatgccc aagaagctca cgaagccatt
     4741 cgcccgtcgg atgtgaatgt caaaatggcg gatttaaaag gaatggataa agatgccgag
     4801 cgtttatacg atttaatttg gcgtcgtttc ttggcgtgtc aaatgttgcc tgctcgttat
     4861 gattctaccg cagtcacagt gtctgccgga gactatgaac tcaaagcaaa cgggcgtgta
     4921 ctgcgttttg acggctggac gaaagtactg ccggtgcaag gcaaaaatcc ggaagatcag
     4981 gaattaccgg cggttgaact gcatcaaaaa ttaacgcttg atgaaattgt gccaagtcag
     5041 cattttacta aaccgcctgc acgttttaat gaggcgtctt tggttgctga gttagaagag
     5101 cgaaaaattg gccgcccttc aacctatgct tcaattattt cgacgattca agaacgtggc
     5161 tatgttcgca ccgaaaacaa gcgtttttat gtggaaaaaa tcggtgaagt agtgacggat
     5221 cgcctcaacg aatcattcaa agatttaatg aattatgatt tcactgccaa tatggaagag
     5281 gtgctcgacc aaattgcaga ggggtctaaa aattggaaag aagagttgaa tactttcttt
     5341 aaacatttct ctgaagagtt gagccaagca gaattagatg aacttgaagg cggaatgaaa
     5401 ccaaacagcc ttgtgccaac cgatattgat tgcccgactt gtggacgaaa aatggcaatt
     5461 cgcaccgctt ccacaggggt atttttaggt tgcaccggct atgctttacc gccaaaagag
     5521 cgttgcaaaa cgacaatgaa tttaattcca gaggcggaat ttttcaatgt gttagattcg
     5581 gattctgaaa ccaaagccct aatggctcgc aagcgttgcc cgaaatgcga ttcggcaatg
     5641 gatagttatg tgattgatgc tgaacgtaaa ttacacgttt gtggtaataa tccaaattgt
     5701 gatggtcata ttattgagca aggttccttc aaaattaaag gctatgacgg acctgtagtg
     5761 gaatgcgata aatgcggttc agatatgcac ttaaaactgg gtcgtttcgg caaatatatg
     5821 ggctgcacaa gctgcgataa cacccgtaaa attctgaaaa acggggaagt tgctccgcca
     5881 aaagaagatc ctattgcttt tcctgaatta aaatgtgaaa aagccgatgc ctattttgta
     5941 ttaagagacg gagcaagtgg ggtgtttatg tcagctcata acttcccgaa agtgcgtgaa
     6001 agccgtgcac caaaagtggt agagttagct caatatcgtg aacgtttgcc ggagaaattg
     6061 cagtatcttg ccgatgctcc gcagcaagat ccggaaggaa acgaggcaat tatccgtttt
     6121 agtcgtaaag agaaacgcca atatgtgact tctgagaaaa acggcaaagc caccaaatgg
     6181 attgttgatt atctagacgg taaatgggtt gagcgtaaga aataaagctt aaaagacaag
     6241 cggtaaaatt ttctaaaaat tttaccgctt gcttttagat gttctgagcg agtagttcat
     6301 caattgcttt acttaattta ttgcgatcgt gtcggtaata aacgtcattc gcagataagt
     6361 tttttcgcaa gatttgaata tgttcgggaa gttcagaacc ggcatcatca gtcgtaatta
     6421 ccgcattaat tctagcctgt ccgatacagt tctccatcca ttcaattcgc tgattaagtg
     6481 atagttgccc agctgggcca tgttcttgtc cgatattgtc aataaatact actcgggctt
     6541 tgctttgagc aatagagttt gctatgcctg ccaccagcaa attcggcata atactggtaa
     6601 aaaagctacc tgggcctagc agtattaact cagcactttg aatagctgaa atggcttctg
     6661 gtgtagcaac aaccatagta tctaacgcta aatgttgtgg tgtttctgct aaagaatcaa
     6721 tttgcacttc accaaatact gattcgcctg aatgcaactg tgcaattaaa tgcaccggta
     6781 tttcagacat tgggataagt tgcgatctta cgtgcaataa ttctcgcacc aaattaatcc
     6841 cttctagcgg tcggatttgc atattttcaa gggcggtgag aattaaattg cctagattat
     6901 gtccagcgag ttcgcctgtg ccagcaaaac gatactcaaa caaccgagaa gctatcgttg
     6961 gtttaacgat aatttgattt aaacagttgc gtaaatctcc ccaagcaatc accccttgtt
     7021 gtgaacgtat tctaccggtt gagcctccat tatcggtagt ggcaacaatg ccggtaagcc
     7081 gttcctttaa aaaagagagc gcggagagca atcgacctaa gccgtggccc ccacctagtg
     7141 caacaactgc attgagttgg tttaaatagt gatgtcgggt aggtttaaaa ggaataatat
     7201 ctgtattttc catcttggga tttcgtttat ttttctaaaa taatggcatc tgactgggct
     7261 gaaggcaatg cttgtaatac agctttaacc aaagtcgcta aaggaatcgc aaaaaacact
     7321 ccccaaaagc cccataaacc accaaagatg agtacggcaa taataattgt cagagggtgt
     7381 agatttactg cttcagaaaa taaaaagggt acgagtaaat ttccgtctag caactgacta
     7441 ataataaatg cgagcattaa atagtagaaa tccggcgata acccgaactg gaatagagca
     7501 attaacatta ccggaatgct gactaaaacc gcaccgatgt agggaactaa cactgaaatc
     7561 cctaccgcta cagaaagtag taacggatag cgtaaatcaa aaaagaggaa aataatataa
     7621 gtgacaaccc caacaattag aatttctaaa aatttacctc gaatatagtt ggcaatttgt
     7681 tgttgcattt caaaccatac tcgtgttgca agtcgacggt tttgcggtaa tactttacta
     7741 aaagaacgca ttaaaacgat tttatctttc agtaagaaaa agaccatgag tgggactaag
     7801 aaggtgtaaa tgcctaagcc aactaagctg atgatagagt taatcgagag tgttagaagt
     7861 gattctccca tactcaaaat attactttta gcggtattca caatggaatc tagggtcgca
     7921 taatctacta attcagggta atgctccggt agggcttgta accaagcatt gagcagatta
     7981 aacatcgaag gtaaatcttg aataaaggta actgcttgat tccaaaggct gggtagtagt
     8041 actacgccta aaaaagataa taaagcgatg aaacctccta gtactagaat gacactcaaa
     8101 gttcgaggta atttgagttt gctcgacaaa aaacggattg gccattccaa taaataggca
     8161 aacacaagtg caaccagaag gggcataagc agatcgctaa aaaaatagat aataccgaaa
     8221 ccgatcaata gaatactaaa cagcacaact atttgcgggt cggtaaattt ttttcgatac
     8281 caatcttgca acatttgaaa catatggatt gcctttaaat tatgcgatga aaattatttc
     8341 tggctacaga ctgcaatgaa ccctagcttt ttgtccgggt cattaaatag tttgtacatt
     8401 ttattaaagg tttcgcgatt ttctggtttt aatgcattgc cgataatttt catggcgcct
     8461 aaaacacctt catcatgaat cagaccttta ggggaaagta gagacatttc tccggaaaag
     8521 gtatcaacat tacggaaacc acaacgttgg aataatgatt tccagtctgc tttagcaagt
     8581 ggagagacag tgatattgat tgcatcacgc aactcggcta ataaagtatc ggagtcttta
     8641 tcttggataa caatatcgtg cgttaataaa aagccatttg gttttaatac acgaaaatat
     8701 tcacgtattg ccttttcttt cgcttctaga ggaagcattg tcagcatggc ttcattaatt
     8761 acaatatcaa aactttcgtc gtcaaatggc agttttgttg cgttggcgcg ctgtacttga
     8821 attaaatgct caacatggtt ttctttgata tttttacgtg ctttttcaag cgcatcttcg
     8881 tctaaatcaa taccaacaat ttggcattca aattgttgag ctaattggat tgcagtagtc
     8941 cccatgttac aggcaacttc gagcactttt ttatttttat caaaatcccc gtttgcaatt
     9001 aaccaatcag ttgctaattt ccccccgggg cgtaaacgag ttttgcctaa acgagctaaa
     9061 aaatgatgac caacttcttc ttttttcata aaaacctctg gatttgcaaa atacgttaaa
     9121 tatatgaccg cttgtattgt gatctatgtt cgttattata ctcaattttt tcattttctt
     9181 atatacaaca tcaacagatt ttgccagaat acaacctcat tttgatatga ggaaaataga
     9241 atgagtaagc aaaagaatga ttacctccac cagcgaggtc aaattaaaga aagtgcagta
     9301 aaagcattag tgactgatcc actttttaga caacgtgtag aaagaaatcg taaagggaaa
     9361 gggagctatc gcagacaaga aaaacacaaa aaaactatct atgggcaaaa agaaacccca
     9421 tttaaaaata ttcggttaga gtctttttaa atggggtttt atttgtagct tattgtagtt
     9481 ttataagcct gataaacact atggtttact gaaataggcc aacaacggtt ctggattttg
     9541 aatataagaa gaaagttctt tatgctctgg gtgacccata tagggaattt gcccaagtaa
     9601 aggcgcatta attttttgtg tcagtagttc aattaattcg tgataatgcc gtagccctgg
     9661 atttactctg tttgcgaccc aacttactaa ttttacacca cgctgcaaaa tttcattcgc
     9721 agtaagtagt gcatggttaa cacaaccttc cttaattccc accactaaaa caaccggcat
     9781 attgttttgc attacccaat cagcaaaata gtaatccttg ttaataggag ttagccagcc
     9841 ataggttcct tctacgacaa catttggata ggcattttct agtcgtttta aatctgtgtt
     9901 taatttttcc agttgaatat gatgaactgc atctaaagct gcaaatacag gggtactgga
     9961 atgaataaaa gtatagctat taatatcacg atagctaaca ttagttggac agctatctag
    10021 tatggttaat acgtctgaat tatcttcagt agcataatca tattgagtag gttctgtggg
    10081 taatgaatca tcaccgccac aagcaatggg tttatagcca attacaggaa aactatgttt
    10141 tgtaagtgtc tgtatgatgg ctcgggtaac cacagttttc cctacgttgg tgtctgttcc
    10201 ggtaataaat aatgagggca taaatggctc ctttcgaaaa tatggctggt agtttaacca
    10261 aaaatagatc aatttattct gtctttgatc aaatttccgt cataaattcc ttgttttatt
    10321 gctgaacagg caattaatgg actattccaa tcgtattggc tggtaactaa atcgattttt
    10381 aggtttgttt gtaacaaatt tttatgcaat ttttctgaaa tttgcgtaaa taacggctct
    10441 tttattttga tcaatggcga gtttag
//
LOCUS       DANLUQ010000083        10315 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.083, whole genome shotgun sequence.
ACCESSION   DANLUQ010000083 DANLUQ010000000
VERSION     DANLUQ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10315
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.083"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            121..2268
                     /locus_tag="PXB27_001606"
     CDS             121..2268
                     /locus_tag="PXB27_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDL5346377.1"
                     /translation="MKRLRYSYLALILGSALPLSAYADGYLSNDNIRQFDARFNFFLA
                     NPLPAPIAKADGRGEAIENLVVKYASDYLNDIYLKEDVIRFRRWQWQDLYTSLDLKTK
                     QKPDYLNTYRLQAEAYFINKQYQEALSQLDQILRRNPNDVHAMAMSVVASRVLENPNT
                     EAERLLALYQISPSIAQKVQQYLHFTDTMLAASYGSEPQTTMIPDTIAVFGQSPNPDG
                     TPSKGLLQRLEKTKEMAEKFPNAKIILSGGPVRYIYAEADVMKKWLIENGVDESRLLL
                     DDVARDTPGNAVGIIDFMKEYGGRKVLGIGTILHLPRAMSVLKSYADSIGYELEIDSA
                     GGGSPPNEKNKKGEALYTFVNVARAMGLFTLGDFENLLAKEQAEKEQREALEKAQQNY
                     LEKLVAERDQAVKNAENSANELEVARQALETAKQAHQQAEEELNKELSKTQEQLKQST
                     EALSRLIIKQNNPFSEHYVVQLNLMEQATYDLYRRLAEIEHTQDWNLWLSDSLSKYRL
                     KNRSTEDYHLLHIGLDHQVSHNWSIGGVFGISQGQIENRTHKVKGTSAGIYAGYRPTS
                     GLFWTNFFNYSHFTNQSGVTKRDNNAYSLSSEIGRNIVMSHQWQITPKLQLGWAKAGS
                     ARMFWAGTGLRLGKVIELNAIKLKPYADISYRFRNRHIDTLDSLSDRHRNVISIGIQA
                     VGNRHYFAIEGSMLHGDALKRSYGLHANYHYQW"
     gene            2281..3399
                     /locus_tag="PXB27_001607"
     CDS             2281..3399
                     /locus_tag="PXB27_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL5346378.1"
                     /translation="MKKIKTTLSALALSLSLISVNSWAIPADYLSQDNISQYDKAFNF
                     VLAKVLPAPKGKEDGRGAPIEHLVIQYAPDYVDDPYLKEDVLNFRRWYWQAMYASLDQ
                     KVKEKPTYLNTYRLQAEAYFVNKEYREALSQLDQILRQNPRDIHALAMTVLASRVLED
                     TEQETARLSALKQLAPEVAQRMTDFFAFTEQTLAASYGNEPQTAMIPDTIAVFGSTPN
                     KDGTPSPLLLDRLQKTKEMAQKFPQAKIIVSGGAVKTEFAEADVMAKWLSENGIEKDR
                     LLLDPVARDTPGNAVGIIKLMQTYNGKKVLGIGTLQHIPRATAVLKGYAQHIGYPIEM
                     DSVGGGKPATPGKAKIEALYTYVNVARSMGLFELGDFK"
     gene            complement(3460..4722)
                     /gene="rho"
                     /locus_tag="PXB27_001608"
     CDS             complement(3460..4722)
                     /gene="rho"
                     /locus_tag="PXB27_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDL5346379.1"
                     /translation="MHLTQLKNTPVSKLVEMGENQMGLENLARLRKQDIVFAILKQHA
                     KSGEDIFGQGVLEILPDGFGFLRSSDSSYLAGPDDIYVSPSQIRRFNLQTGDKIEGKI
                     RPPKEGERYFALLKVDSVNDDKPEASRSKILFENLTPLHANSRLKMERGNGSKEDLTA
                     RILDLAAPIGKGQRALIVAPPKAGKTVLLQNIAQSITHNHPECELIVLLIDERPEEVT
                     EMQRTVRGEVIASTFDEPATRHVQVAEMVIEKAKRSVEHKKDVVILLDSITRLARAYN
                     TVTPVSGKILSGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVDTGSKMDEVIFE
                     EFKGTGNMELHLSRRIAERRIFPAIDFNRSGTRKDDLLMAADELQKTWMLRKVLNPMD
                     EVDAIEWLIDKLMMAKTNEEFFEIMKRS"
     gene            complement(4908..5855)
                     /gene="cysK"
                     /locus_tag="PXB27_001609"
     CDS             complement(4908..5855)
                     /gene="cysK"
                     /locus_tag="PXB27_001609"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015939738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDL5346380.1"
                     /translation="MTIYADNSYSIGNTPLVRLKNFGNGNIVVKVEGRNPSFSVKCRI
                     GANMVWQAEKDGILTKDKEIVDATSGNTGIALAYVAAARGYKLTLTMPETMSTERKRL
                     LRGLGVNLVLTEGAKGMKGAIAKAEEIAASDPDRYVTLKQFENPANPAIHQQTTGPEI
                     WDATEGKIDVLVAGVGTGGTITGVSRAIKQDKGKQIISVAVEPSESPVITQTLNGEEV
                     KPGPHKIQGIGAGFIPKNLDLSLLDRVEQVSSEEAITTARRLMAEEGILGGISSGAAV
                     AAADRIAKLPEFADKLIVVILPSASERYLSTLLFEGIEA"
     gene            complement(6029..7525)
                     /gene="pepA"
                     /locus_tag="PXB27_001610"
     CDS             complement(6029..7525)
                     /gene="pepA"
                     /locus_tag="PXB27_001610"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HDL5346381.1"
                     /translation="MEFSVKNGSVEKQRTACLVVGVYEPRRLCSAAEQLDKLSNGYIS
                     TLLRRGDLEGKAGQTLLLHNVPNVLADRVLLVGCGKERELTERQYKQIIQKSVQTINE
                     TGSMEAVSFLTELHVKGRTPYWNVRFAVEAIQEQLYAYNDFKSIKPEVRRELRRVIFN
                     VAHRKDLPDAERALEHGKAVALGISHAKNVANCPPNVCNPHYLAELAKGLAYDYPAIS
                     TTIVDEKQMAELGMNAYLAVSRASENEAFLSVIEYKGNPNADAKPIILVGKGMTFDSG
                     GLSIKPAEAMDEMKYDMCGAATVYGTIKAIAEMKLPLNVIGLLAGCENAVDGKAYRPG
                     DILTTMSGLTVEVLNTDAEGRLVLCDALSYVERFEPELVIDIATLTGACMIALGNHNS
                     GLMSPHNGLANELLAAAEQADDKAWRLPLGEEYQEQLKSNFADLANIGGRLGGACTAG
                     QFLSNFTKKYTWAHLDIAGTAWKSGAAKGATGRPVSLLTQFLINKAQV"
     gene            7763..8560
                     /locus_tag="PXB27_001611"
     CDS             7763..8560
                     /locus_tag="PXB27_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346382.1"
                     /translation="MRFWGKTFVVGLIGLGTQNTIFANDDAQISRYEQAKLRLSYDGN
                     VGNLLQQLSQRLKVGFIAYDVDITRKISLQNETETAIKTINEQIESQLTNIDVRFEKI
                     GERLFLVVSAKDVAPLLQNEPKEVQFVGDVIFDGEPNLTEQAQSTAEATKVQNILNIA
                     TNQEKIMAAKGKKAPQYKTVTKDNIGLKNIRVTALGTFLVFDTSVDAAKLRVKGNFED
                     IAQGENIIAILHQNTEAPPKIEIEDSQGKKLILEAHKATSSKKSKKQ"
     gene            8734..9843
                     /gene="lptF"
                     /locus_tag="PXB27_001612"
     CDS             8734..9843
                     /gene="lptF"
                     /locus_tag="PXB27_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HDL5346383.1"
                     /translation="MILSRYLTKEIFKSQLAILFILLLIFFCQQLVRVLSSAVSGKVP
                     ADLVVSLLGLGMPTMAQLMLPLSLFIALLLTLGRFYAESEITVMRACGIGQGLLVKVA
                     MFLSIFTTALAVYNVFWLTPWAINKQSEMLAEAKANPRFAALSAGQFMSAGGYVLFID
                     KIENNSLNDIYVFQPEQQKKNKPSVVVAESGTLQGLPNGDQVLTLAESKRFEGTPQSA
                     DFRISHFENYSAYLGYQDVESDEKLVQRADFKKLMETKTPEAKAELHWRLALILAVPL
                     MALLAVPMSSVNPRQGRFAKLLPALLLYLIYFLLQSSLKSSGASGKLNAEVLMPLVSV
                     AFLVLGVILNAWDSKFMSKLRYQFGAKKLTNKAVA"
     gene            9843..>10315
                     /locus_tag="PXB27_001613"
     CDS             9843..>10315
                     /locus_tag="PXB27_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HDL5346384.1"
                     /translation="MQFFSMNILERYIGKTILSAIFLTLFMLIGLGAIIKFVEEFRDV
                     GRGSYDGLKAAYYTFLTIPRDIETFFPIAALLGSLLGLGNLASLSELVVMQASGFSRF
                     RIGLAVMKTALPLVIFTMIIGEWGVPQTEQFARNMRSIAQSGGSMLATKDGFWAK"
BASE COUNT         3016 a         1963 c         2217 g         3119 t
ORIGIN      
        1 aaaaatgatt ttgaatatta ttaataaaat agcacaaaca aattttatcc ttaatcaact
       61 ttgttggtta gatgtaaaaa gttataaata cttcttcacc caatataagg agaaatcata
      121 atgaaacgct tgcgttattc ttatcttgct ttgatattag gttcagcctt acctttatcg
      181 gcttatgctg acggttattt aagtaacgat aatattcggc aatttgatgc ccgttttaat
      241 tttttcctcg caaatccact acctgcgcct attgcaaaag cagatgggcg tggtgaagcg
      301 attgagaatt tggtggtgaa atatgcatca gattatctta atgatatcta tcttaaagag
      361 gatgttattc gttttcgccg ttggcagtgg caagatctct atacgtcatt ggatttaaaa
      421 acgaaacaaa agcccgatta tttaaatact taccgattac aggcggaagc gtactttatc
      481 aataagcaat accaagaagc attatctcaa ctggatcaaa ttctgcggcg taaccctaat
      541 gatgttcatg caatggcaat gagtgtggtt gcaagtcggg tattggaaaa tcctaatact
      601 gaagccgagc gtttattagc gctttatcaa atctctccaa gtattgcgca aaaggtgcag
      661 caatatctac attttacaga cactatgctt gctgccagct atggtagtga gcctcaaaca
      721 acaatgatcc ccgacaccat agcggtattt gggcaatcgc ctaacccaga cggtactcca
      781 agcaagggat tattgcaacg tcttgagaaa accaaagaaa tggcagagaa atttccgaat
      841 gcaaaaatta ttttaagtgg tggacctgtt cgttatatct atgcagaagc ggatgtaatg
      901 aaaaaatggt tgatagagaa tggcgttgat gaaagcagac tgttgcttga tgatgtggca
      961 cgagatactc cgggaaatgc tgtcggtatc attgatttta tgaaggaata tggaggtaga
     1021 aaagtattgg ggatcggtac gatcttacat ttaccgagag caatgtctgt attgaaaagc
     1081 tatgcggatt ccattggata tgaattggaa attgattctg ccggaggcgg ttctcctcct
     1141 aatgaaaaaa ataaaaaagg tgaagctctt tatacatttg ttaatgttgc tcgtgcaatg
     1201 gggttattta ccttgggcga ttttgagaat ttgcttgcca aagaacaagc tgaaaaagaa
     1261 caaagagaag cgttggaaaa agcacagcag aattatttag aaaaattggt agctgagcga
     1321 gatcaagcag taaaaaatgc agaaaattct gcaaatgagc tggaagtagc cagacaggcg
     1381 ttagaaacag caaaacaggc tcatcagcaa gctgaagagg aattgaataa agaacttagt
     1441 aaaacgcaag agcagttaaa acagagtacg gaagccctta gtcgtttaat aataaagcag
     1501 aacaatcctt tttccgagca ttatgttgta cagcttaatt tgatggaaca agcaacttat
     1561 gatctgtacc gtaggttagc agaaatagaa catacacaag attggaattt atggctttcg
     1621 gatagtctat ccaaatatcg tttaaaaaat cgttcaaccg aagactatca cttattacat
     1681 atcgggctag atcatcaggt ttcccataat tggtctattg gtggagtatt tggtatcagt
     1741 caggggcaaa ttgagaatag gacgcataaa gtgaaaggga catctgctgg tatttatgct
     1801 ggttatcgtc ctacaagtgg tctgttttgg acaaattttt ttaattacag tcattttact
     1861 aatcaatctg gtgtaacaaa acgagataat aatgcataca gtctgagtag tgaaattgga
     1921 agaaatatcg tgatgagtca tcaatggcaa attacgccaa agctgcaatt aggctgggca
     1981 aaagctggat cagcacgaat gttttgggct gggacagggc tacgtttagg aaaagtgatt
     2041 gaactcaatg caatcaaact taaaccttat gctgatatta gctatcgttt tcgaaatcgt
     2101 catattgata ccttagatag tttatctgat cgtcatcgga atgtaatcag tattgggata
     2161 caagcagttg gaaatcgcca ttattttgca atcgaaggat caatgttaca cggggatgca
     2221 ttgaagcgtt catacggatt acacgcaaat tatcactatc aatggtaaat aggagcctct
     2281 atgaaaaaaa tcaaaacaac cctttctgcc cttgctttaa gcctttcttt gataagtgta
     2341 aatagctggg cgataccagc tgattatttg agccaagaca atatcagtca atatgataag
     2401 gcgtttaact ttgtattagc aaaagtactg cctgcaccaa aaggtaaaga agatggtcga
     2461 ggagcgccga ttgagcattt agttattcaa tatgcgccag actatgttga tgacccttat
     2521 ctaaaagagg atgtgctgaa tttccgccgt tggtattggc aagcgatgta tgcttcgctt
     2581 gatcagaaag ttaaagagaa gccgacctac cttaacactt accgtttaca agcagaggcg
     2641 tatttcgtca ataaagagta tcgggaagca ctttctcagc tcgaccaaat tttgcgccaa
     2701 aacccccgtg atattcacgc tcttgcgatg acagtattag cttcaagggt gctagaagat
     2761 actgaacaag aaacggctcg tctttctgca ctgaaacagc ttgctcccga agtcgcacaa
     2821 cgaatgacag acttttttgc ctttaccgaa caaacgctgg cggcaagcta cggtaatgaa
     2881 ccacagacag caatgatccc tgatacgatt gccgtttttg gttctacgcc aaacaaagac
     2941 ggcacacctt cgccgctgtt gttagatcga ttacagaaaa ccaaagagat ggcacaaaaa
     3001 tttccgcaag caaaaattat cgtcagtggc ggtgctgtga aaacggaatt tgctgaggct
     3061 gatgtgatgg caaaatggct tagcgaaaat ggtattgaaa aagaccgttt gctacttgat
     3121 cctgttgcca gagatacccc gggaaatgcg gttgggatta tcaagctgat gcaaacttat
     3181 aatggcaaga aagtattagg tatcggtacg ttacaacata tccctcgtgc aacagcggtt
     3241 ctcaaaggtt atgcacagca cattggttat ccgattgaga tggattccgt tggtggtggc
     3301 aagccggcaa caccgggaaa agccaaaatc gaggcacttt atacttatgt aaatgtggcg
     3361 agatcaatgg ggttgtttga gttgggggat tttaagtagt tactttattg acagctgcta
     3421 ttgataaaaa tcccgcgtta agcgggattt ttttatgttc tacgaacgtt tcataatctc
     3481 gaaaaactct tcgttagttt ttgccatcat taatttgtcg attagccatt cgatagcatc
     3541 tacttcgtcc attgggttga ggactttgcg taacatccaa gttttttgga gttcgtctgc
     3601 tgccattaag aggtcatctt tacgagtacc tgagcggttg aagtcaatag ccgggaagat
     3661 acggcgttca gcaatacggc gtgagaggtg taattccata ttacccgtac ctttgaactc
     3721 ctcgaaaatc acttcgtcca tttttgatcc ggtatccaca agtgcggttg cgataatggt
     3781 taagctgcca ccttcttcca cattacgggc tgcaccgaag aaacgttttg gacgatgtaa
     3841 ggcgttagcg tccacaccac cggaaaggat tttgccggaa accggggtaa cggtgttgta
     3901 ggcacgggct aaacgagtga tggagtctaa taaaatcacc acatcttttt tatgctctac
     3961 cgagcgtttt gctttctcga ttaccatctc cgcaacttga acgtggcggg ttgccggttc
     4021 atcaaaggtt gaggcaatta cttcaccacg tacggtgcgt tgcatctctg ttacttcttc
     4081 cggacgctca tcaattaata atacgataag ctcacattca ggatgattat gggtgatgct
     4141 ttgtgcaata ttttgcagta acacggtttt accagctttt ggcggggcaa caattaacgc
     4201 tcgctgacct ttaccgattg gggcggctaa atctaaaata cgagcggtta aatcttcttt
     4261 tgatccattg ccacgctcca tttttaagcg agaattggcg tgcagagggg ttaagttttc
     4321 aaagagaatt ttactgcgag aggcttccgg tttgtcatca ttaactgaat caactttcag
     4381 tagggcaaaa tagcgttctc cctcttttgg tgggcgaatt ttaccttcta ttttatcgcc
     4441 agtttgtaag ttaaaacggc ggatttggct tggagaaaca taaatatcat cagggccggc
     4501 taaatatgag ctatctgatg agcggaggaa accgaagccg tccggtagaa tttcaagcac
     4561 gccttgcccg aaaatgtctt caccgctttt tgcgtgttgt ttaagaatag caaatacgat
     4621 gtcttgttta cgtaaacgag ccagattttc taagcccatt tgattttcac ccatttccac
     4681 aagctttgaa acaggggtgt ttttaagttg agtaagatgc ataatttgaa tgtaaaattg
     4741 tgattaatta agattggatt tgttgatatt gattagatac ggccaaccgt aaagatgtgg
     4801 cgaagaataa cgcaatagga gacaaattgc aaatttttta taaaaaataa ccgcttgttt
     4861 gcaataaaaa tgccgacatc agccggcatt tttatattaa tttaatatta cgcttcaatc
     4921 ccttcaaaca gtagcgtact taaataacgc tctgaggctg aaggcagaat cactacaatt
     4981 aatttatcgg caaattcagg tagtttagcg atgcgatctg cggcagcaac ggcagcacct
     5041 gatgaaatac cgcctaaaat gccttcttct gccattaaac ggcgtgcggt tgtaatcgct
     5101 tcttcgcttg aaacttgttc aacacggtca agcagtgata aatctaagtt tttcggaata
     5161 aagcctgcac caatgccttg aattttgtgc gggcccggtt tgacttcttc accgtttaaa
     5221 gtttgcgtaa tgaccggcga ttcactcggt tctaccgcca ctgaaataat ttgcttgcct
     5281 ttatcttgct taatagcacg ggaaacaccg gtaatagtgc cacctgttcc tacgcctgcc
     5341 actaatacat caattttacc ttcagttgca tcccaaattt caggaccggt agtttgttgg
     5401 tgaattgccg ggtttgccgg gttttcaaac tgttttaagg taacatagcg atctgggtcg
     5461 gaagccgcga tttcttcagc tttcgcaatc gctcctttca ttccttttgc cccttcggtt
     5521 aagactaaat ttactcctaa gccacgcaat aagcgtttac gctcggtact cattgtttcc
     5581 ggcattgtga gcgttaattt ataaccacga gcggctgcta cataagccaa ggcaattcca
     5641 gtgttaccgc tggttgcatc tacaatttct ttgtctttgg ttagaatacc gtctttctct
     5701 gcttgccaca ccatattcgc accaatacgg catttcacgc taaaacttgg gttacgccct
     5761 tccactttta ccacaatatt gccgttgcca aaattcttta aacgcaccag cggggtattg
     5821 ccgattgagt atgagttgtc tgcatagata gtcatagtga tattccttaa taagtattga
     5881 gattgggatt tatcatactt atttttaatc gcttttctag tagctggaat ttatttgata
     5941 taactaaaaa gaatataggg gcgtatgaag aggatcggtc tgattttctt tttgcaaaaa
     6001 ctcgataaaa tcagaccgct tgtgagagtt agacctgggc tttattgatc aaaaattgtg
     6061 ttagtaagga taccggtctg ccggtcgccc ctttggctgc cccagatttc caagccgtgc
     6121 cggcaatatc tagatgcgcc caagtatatt tctttgtaaa gttagagagg aattgaccgg
     6181 cagtacaggc tccgcctaaa cgtccgccga tattagcaag atcagcaaag ttagatttta
     6241 actgttcttg atattcttcc cctaaaggta aacgccacgc tttgtcatcg gcttgctctg
     6301 cggcagctaa gagttcgttt gctaaaccgt tatgaggtga catcagcccg ctgttatgat
     6361 tacctaaggc aatcatgcaa gcaccagtta gggtagcaat atcaatcact aattccggtt
     6421 caaaacgttc tacatagctg agagcatcac atagcactaa acgtccttct gcatcggtgt
     6481 tgagtacctc aacggttaaa ccagacattg tagtgagaat atcccccggt cgataggctt
     6541 tgccatcaac cgcattttca cagccggcga gtaagccgat aacattgagt ggtaatttca
     6601 tctcggcaat tgctttgatt gtaccgtaaa cagtagcagc tccgcacata tcgtatttca
     6661 tttcgtccat cgcctccgca ggtttaatgg ataaaccgcc agagtcaaag gtcattcctt
     6721 tgccgactaa gataatcggc ttcgcatcag cattcgggtt gcctttatat tcaatcactg
     6781 aaagaaaggc ctcattttca gaggctctgg aaaccgctaa gtaagcattc attcccaatt
     6841 ctgccatctg tttttcatca acaatggtag ttgaaatggc aggatagtcg taggctaaac
     6901 ccttagccaa ttcggctaaa tagtgaggat tacacacatt aggcggacaa tttgctacat
     6961 ttttagcgtg gctgattcca agtgctacgg cttttccgtg ttctaaagct cgttcggcat
     7021 caggaagatc tttacggtgt gcgacattaa aaatcacacg gcgtaattca cggcgaactt
     7081 caggtttgat gcttttgaaa tcgttatagg catatagttg ttcttggatt gcctctaccg
     7141 caaatcgcac attccaataa ggtgttctac ctttaacgtg cagttcggtt aaaaagctga
     7201 cagcttccat tgaaccggtt tcattaatag tttgtacact tttttgaata atttgtttgt
     7261 attggcgttc agttaattca cgctcttttc cacagccgac taataatact cgatcagcga
     7321 gtacattcgg cacattatgc aagagtaagg tttgccctgc tttgccttct aaatctcctc
     7381 ttctcagtaa tgtgctgata tagccgttac ttaatttatc aagctgttct gcggcagaac
     7441 ataatcggcg gggttcatag acaccaacaa ccaaacaggc ggttctttgt ttctcaacac
     7501 tgccattttt gacactaaat tccatttttc ctccttaatt tttattgcta cagattttat
     7561 aatgccaaca taatcatcaa tgataagtaa aatatagata taccctgatg taaatcatta
     7621 tttaaaataa tttacttttt atttgcaaat attaaatgtt ttttgcggtc gtatagataa
     7681 ttttactggt ttttctttta gtttgcttac tttatactaa aagagttttt tagatttaag
     7741 agaataattg gagataaaaa tgatgcgctt ttggggtaaa acgtttgttg ttggtctaat
     7801 cggcttaggg actcaaaaca ctattttcgc taatgatgat gctcaaatta gtcgctatga
     7861 gcaggcaaag ttacgtcttt cttatgatgg aaatgtgggc aatttacttc agcagttatc
     7921 acaacgttta aaagttggtt ttattgctta tgatgttgat attacacgta aaatatcgct
     7981 tcaaaatgag acagaaactg cgatcaagac aattaatgaa caaattgaat cacaattaac
     8041 caatattgat gtccgttttg aaaaaatagg tgaacggtta tttttggttg tttcagctaa
     8101 agacgttgca ccattattgc aaaatgagcc taaagaggtg caatttgttg gggatgttat
     8161 ttttgatgga gaaccaaatt taacagaaca ggcacaatct acagcagaag cgactaaagt
     8221 tcaaaatatt cttaatattg cgactaatca agagaagatt atggctgcaa aagggaaaaa
     8281 agcacctcaa tataaaacgg taacgaagga caatataggg cttaaaaata ttagagttac
     8341 cgcattaggt acattcttag tatttgacac tagtgttgat gcggctaagt tacgtgtgaa
     8401 aggaaacttt gaagatattg cacaaggtga aaatattata gctatccttc atcaaaatac
     8461 ggaagcacca cctaagattg aaattgaaga tagccaaggt aagaaattaa ttcttgaagc
     8521 tcataaagca acatcatcga aaaaatcgaa aaaacaataa tacttacgca cggttttgcc
     8581 gtgcgtgata ttttctatag gtgatttttt tgtatgaaaa aggtattatt agcgattcga
     8641 ataagttatt ttgcgctctt tgcaaaaaat taacaaaaat agaccgcttg tgtttagcgg
     8701 tattcagatt tgtaaatgtt atagagaaaa aacgtgatct taagccgata tttaaccaaa
     8761 gagatattta aaagccaatt ggcgatattg tttatcttat tactgatctt tttttgtcag
     8821 caattagtgc gtgttctaag ttctgctgtg agtggtaaag taccggcaga tttagtggtg
     8881 agcttattag gcttaggtat gccgacaatg gcacaattaa tgctgccact ctcactgttt
     8941 atcgccttgt tattaaccct cgggcggttt tatgccgaaa gtgaaattac cgtaatgcgg
     9001 gcttgtggta tcgggcaagg gctattggtt aaagtggcga tgtttctctc gatttttacc
     9061 actgcattag cggtttataa cgtgttttgg cttacgcctt gggcaattaa taaacagagt
     9121 gaaatgttag cggaagcgaa agccaaccca cgttttgcgg ctctttcggc cggacaattt
     9181 atgtctgccg gtggctatgt gttatttatt gataaaattg aaaataacag tctaaatgat
     9241 atctacgttt tccagccgga acagcagaag aaaaacaaac cttcagtggt tgttgctgaa
     9301 tccggcacgt tgcaaggttt gccaaatggg gatcaagtct taaccttggc agagagcaag
     9361 cgttttgaag gtacaccaca aagtgccgat ttccgtattt cacattttga gaattattct
     9421 gcttatttag gctatcaaga cgttgaatct gatgagaaat tagtgcaacg agccgatttc
     9481 aagaaattaa tggaaaccaa aacgccggaa gcaaaagccg agcttcattg gcggcttgct
     9541 ttgattttag ctgtaccgtt aatggcatta ttagcggtgc caatgagtag tgtaaaccct
     9601 cgtcaagggc gttttgctaa actactgccg gcgttattgc tttatttaat ctatttctta
     9661 ctacaaagct cgctaaaatc gtcaggggca tcaggcaagt tgaatgcgga agtgttaatg
     9721 ccgttggtat cagttgcctt tttagtttta ggtgttattt taaatgcgtg ggatagtaaa
     9781 tttatgtcta agttacgcta tcaatttggt gcaaaaaaat taaccaataa ggcggtggca
     9841 taatgcaatt tttctcaatg aatattttag aacgctacat cggcaaaacg attttatccg
     9901 cgattttttt aaccctgttt atgttgattg ggcttggggc gattattaaa tttgtggaag
     9961 agtttcgtga tgtaggacgt ggatcttacg atggcttaaa agcggcatac tatacttttt
    10021 taactattcc acgagatatc gaaacctttt tcccgattgc ggcattactt ggctcattac
    10081 tcggtttagg caacctagcg agccttagtg aattggtggt gatgcaagcc tccggttttt
    10141 cccgtttccg cattggttta gccgtgatga aaactgcctt accgttagtc atttttacga
    10201 tgattatcgg cgaatggggc gtgccacaaa ccgagcagtt tgcccgtaat atgcgttcga
    10261 ttgcccaaag tggcggctca atgcttgcca caaaagacgg gttttgggcg aaaga
//
LOCUS       DANLUQ010000084        10147 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.084, whole genome shotgun sequence.
ACCESSION   DANLUQ010000084 DANLUQ010000000
VERSION     DANLUQ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10147
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.084"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..576
                     /locus_tag="PXB27_001614"
     CDS             <1..576
                     /locus_tag="PXB27_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL5346385.1"
                     /translation="GAYLEASEAQIFRGKYQEMEFVPNPDFDGPYYGLDFGFAQDPTA
                     AVKCWVFNGDLYIEFEAGKVGLELDDTADFIAKGVPQIADHVVRADSARPESISYLKR
                     HGLPRIIGVEKWKGSVEDGIEHIKSYGKVFIHPRCQQTLNEFRLYSYKTDRLSGDVLP
                     VVIDAHNHFIDALRYALTPLMQVKSAKGVLL"
     gene            573..1949
                     /locus_tag="PXB27_001615"
     CDS             573..1949
                     /locus_tag="PXB27_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HDL5346386.1"
                     /translation="MSLEQDRLAFLAEALGLGNIKRRTLWKEFGYPNTLTFNHFLKAY
                     KRNSIAFAAINRLLDGCWVDCPVIVEGEQKNESKQTTEWESKVERFMKRYWSAIKEAD
                     RRNLVGNYSALLLQVRDGQQWDQPILQGALSSIGEFGLVKLIPVWQSQLSVTEFQEDV
                     TADNYGEPLMYQFSESAFGKKSVSRNIKVHASRVILLNEGGDFNSPHSGVPLLEAGYN
                     KLVDLEKTSGGSAEGFLKNASRQLGIKLTKDVDLRQLEDSAKALGFSSFSDALNDKIK
                     KINSGTDSALITHEGDASVLSVAPADPKPTWEISANEFAASVQIPFTILFGQQTGRLA
                     SDEDKTDWANRCNGRRNGFLTDVITQLLNRLWFIGVLPMPKNSDVTVSWSDLLAPSEK
                     EKIANAQALASVATTSQSAFGFAAVTANEIRESLGFEPLPDNLIPPTIDDENEDQDEN
                     QATADSDK"
     gene            1903..2841
                     /locus_tag="PXB27_001616"
     CDS             1903..2841
                     /locus_tag="PXB27_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HDL5346387.1"
                     /translation="MKIKTKIKPLRIPTNKADPLNMGVSVAKLFRHIDVIYSQIKRSI
                     KQQILQKMSLRLQANNFEYFPSYLTATELADLLETLQKIIDKEILSNGSQGENLWFDS
                     YIDEAALKGTQSAVTDLGLQSEAYRSQRNLSSIVFSPAYFNRLAIAHMASYSEWQGLS
                     DELRKSLAGVITEAVLNGKNVKETAREIRQKLDVSSKRAKRIAQSSQLAAYRRAEWQE
                     AEEAKEELGLNTKLLHFSALKATTRLAHAQRHGKYFDVAEVREWYSRDGNQFNCYCKQ
                     SVVVVNADGKTDIEPLLVGLAKERKQWVGALKNVKK"
     gene            2828..4198
                     /locus_tag="PXB27_001617"
     CDS             2828..4198
                     /locus_tag="PXB27_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016533290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HDL5346388.1"
                     /translation="MSKNNVNIVTVINSKNISTESIDGDQHLIIRGVVPIVDDVVMND
                     GLYPADEINKSYNSLEGNFMPLGHPKIEGKYVSAQDVRAVNKHHVGAWAKNVRKESGK
                     VLVDMYINRRFAEGSEQGKTLLERLDGMVTNTNAEPIHVSTGLILNKVKQSGKSKGKK
                     YSWIATNMSFDHIAILLDVPGAATPDDGVGIFVNQDGSEMGVENVNLTQAADYRTEKL
                     FDKVRFYLMANSDLSFEEIHRALREAIRPEGKDKWSFWVEAVYPKYFIYSDDNSGKKY
                     KRSYFIDENDNVQLTSEPVEVVKKIGYEEITTNEEHNPMKTKILAVLNAANVKTEGLN
                     DDQLLEAYNKLQADKGKEPEKPKNEDDSEIDEKIKKAVNAAIEPLQQALQANTDKEQA
                     AMRAAVKAKFNMSDAAVNALQGEVLTELYAQTQQSVGLNNNSANGNGDNQWDGYSLNQ
                     EEKGNE"
     gene            4191..4625
                     /locus_tag="PXB27_001618"
     CDS             4191..4625
                     /locus_tag="PXB27_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346389.1"
                     /translation="MSNVIYRGSVKREPQTVNIVINDTSAPGAVVKLHNGKLEAAADL
                     KGRRFLLGNNRFKGQTIEQAYAKGDTATAFRLEPEQEYYAQIADGTYNFGDELTVKQA
                     GGKLTKAETGDVVLFFFDEESQRVISGGKGYADVVVANAYTK"
     gene            4640..5629
                     /locus_tag="PXB27_001619"
     CDS             4640..5629
                     /locus_tag="PXB27_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="encapsulin"
                     /protein_id="HDL5346390.1"
                     /translation="MLKFTKEQERFVINERINYDKKHALMAANSAGLLGNAMTLPKDV
                     WAEWDRDAVQIQREELVVFNDLAGISKSMPIGKLIHHFQTVSDSGSVNISLDGRSSAK
                     TDNVVLNYHGTPLPIIDSTFSFGWRDMSAAQSEGYQIDGAARANSLRKVAEKMEDLAL
                     NGDSSIVVGNAKLYGLRTAPNRMTNTHNLDLATATPKEIYDVFRDLISKFHAKNYYSP
                     VTLYVNYGDYFAMSTRDYSEQKSEGSILNKVMTIPQIAKIVPASRVPQNEILGLCKRS
                     DVYQVLNGMPLVTRPIARHNETDDYSFQIMAAVAVEFKFDDKGNAGYIQYTKS"
     gene            5641..5901
                     /locus_tag="PXB27_001620"
     CDS             5641..5901
                     /locus_tag="PXB27_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346391.1"
                     /translation="MKWLLTHDSHELKKGDVYEGETLPLWLVGKAIEIEEKSFEVATP
                     SDHADGETGETGETGETGETGETGETGETGETGQKGKKDKGK"
     gene            5904..6281
                     /locus_tag="PXB27_001621"
     CDS             5904..6281
                     /locus_tag="PXB27_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346392.1"
                     /translation="MAAEIELSEARTTLDELGFTSPNSLLELYVQQTNTLDQALNKSG
                     YSEETQKLIKLYLVAILAISSGARRIKSEGAPSGASRTFEYDPNALDNLKSAIRSIDP
                     KGCAESLLPKDKVVGFFDVVGGC"
     gene            6281..6625
                     /locus_tag="PXB27_001622"
     CDS             6281..6625
                     /locus_tag="PXB27_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346393.1"
                     /translation="MSNLSNWSYTAKATLWQAKGKNDDGVLVFSTPQIISCDYGADRK
                     RARFEIGREQAVKNVIWTEFAHAKLGDYVLIGESEQADPILAGAEEIIHIQRFADTFD
                     RNRDDFALITGG"
     gene            6627..6995
                     /locus_tag="PXB27_001623"
     CDS             6627..6995
                     /locus_tag="PXB27_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HDL5346394.1"
                     /translation="MGIKVKGIRQVKQRLNQVVGEVQSQKATRAMFRILNAVAPLAAH
                     YTPVDTSTLINSQFTEMEVNGTRLIGRVGYSANYAAYVHDPNIKQNFRKPSAKKEFLT
                     SALNESKATIKSIVKEELSL"
     gene            6992..7372
                     /locus_tag="PXB27_001624"
     CDS             6992..7372
                     /locus_tag="PXB27_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346395.1"
                     /translation="MISYVKAFKQWLEAHSLADGYVVQLYQWEDTQKAKPVIVIQPNS
                     GSPQVSDLSSEHYLLISLVAGKNSGYTIEERARAIMSKILVEPFASFGYIESMGGLPA
                     PIFTEDNRMIFRLPLRIISTNQEE"
     gene            7376..7858
                     /locus_tag="PXB27_001625"
     CDS             7376..7858
                     /locus_tag="PXB27_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5346396.1"
                     /translation="MGSPSPQNNAENLVVGRAVVLEYGTTDAKPQESEWKVAGAMTTK
                     SWDFSPNSVTSEADDAGGFPETLITNSDFSISGDGEWRKRPKSKELGIKDLVVLYANA
                     VKNRTQPYIWVRLKYAGLTILGKMVITALSSEAPTNDLVKFSIEFKVGDSSTIEIAAA
                     "
     gene            complement(7898..8314)
                     /locus_tag="PXB27_001626"
     CDS             complement(7898..8314)
                     /locus_tag="PXB27_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDL5346397.1"
                     /translation="MFYPALFTPAEEGGFVVTFPDLPEAITQGDTFEEAMEMAEDVLL
                     SCVEIYFDEDRRFPLTRPVNTDEVPVFMPESIYAKVLLHNTMQEQAVSKAEIARLTNI
                     RPPEVQRILAPRHITKIDTIGRILASLGKPLQLSLG"
     gene            complement(8370..8546)
                     /locus_tag="PXB27_001627"
     CDS             complement(8370..8546)
                     /locus_tag="PXB27_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010848235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDL5346398.1"
                     /translation="MKQSEFLRWLKANGVEVENGSKHLKLYYKGKRSHLPKHPSQELK
                     TGLVEGVKKQLGLK"
     gene            8642..9313
                     /locus_tag="PXB27_001628"
     CDS             8642..9313
                     /locus_tag="PXB27_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346399.1"
                     /translation="MKMVSYPITEIGEMLIATDGRDFLFRPSFKNIYKIGSPQEIVKI
                     YALLHGCEIREILPHILTKSESHQSYFWSVLNSPVFGRKILQNAMIVMSCCCDDDISL
                     LIGEWLPSKSGVRYKLGKMPPETIINFAKNLMGHGVIGCCELPKKEQSAKNEFSESFD
                     AVEYISLARTHFNMSREDAENLTMTELQQLLKSQMPAENNSYGQFTEEEYDRIMAEYE
                     KRRAA"
     gene            complement(9328..9561)
                     /locus_tag="PXB27_001629"
     CDS             complement(9328..9561)
                     /locus_tag="PXB27_001629"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346400.1"
                     /translation="MKKDCTKQDFANWLNENQEVCWIKEVSIERILSCVDDYVAICNA
                     RSKDEFELLNDIRELFYSAHYKDFTPVLSGRFF"
     gene            9730..>10147
                     /locus_tag="PXB27_001630"
     CDS             9730..>10147
                     /locus_tag="PXB27_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346401.1"
                     /translation="MKKMIVFVASVLSSFVIASDWEIIEAAQNKVKAELIRKSEKKAE
                     LCLNVAQAVLITNQKVQKDTFSMCGEFSTEIVFSDSKAYSLAVCGKVKGKDIIGNPIE
                     SDYIYQRATKDFYFNYNGKKSAYYDPQSDLARLQEVS"
BASE COUNT         3036 a         2095 c         2409 g         2607 t
ORIGIN      
        1 ggtgcttatt tggaagcgag cgaagcgcaa atctttcgcg gtaaatatca agaaatggaa
       61 tttgtgccga accctgattt tgatggccca tattacggct tagattttgg tttcgcgcaa
      121 gatccgacgg cggcggtcaa atgctgggta tttaatggcg atctgtacat cgagtttgaa
      181 gcaggcaaag tgggcttaga acttgacgac accgcagact ttattgccaa aggcgtgcca
      241 caaattgccg atcacgttgt gcgtgcagat tcggcgcgtc ctgagtcaat tagttacctc
      301 aaacgtcacg gcttaccgcg cattatcggt gtggagaagt ggaaaggctc ggtggaagac
      361 ggcattgagc atattaagtc ctacggcaaa gtctttattc acccacgctg tcagcaaacg
      421 ctcaacgaat ttcgccttta ttcgtacaaa accgaccgct tatcgggcga tgtattgcct
      481 gtcgtgattg atgcccataa ccactttatt gatgcgctac gctacgcatt aacgccgttg
      541 atgcaagtta aaagtgccaa aggagtttta ctatgagcct agagcaagac agactggctt
      601 ttttagccga agccctaggg cttggcaata tcaaacgaag aacgttatgg aaggagtttg
      661 gctaccctaa taccttaaca ttcaaccatt tcttaaaagc ctataagcgc aatagtatcg
      721 cttttgcagc gattaaccgc ttacttgacg gttgttgggt tgactgcccc gtaatcgttg
      781 agggtgagca aaagaacgaa tcaaagcaga caaccgaatg ggagtccaaa gttgaacgtt
      841 ttatgaaacg ctactggtcg gcaatcaaag aggcagatag acgaaacctt gtcggtaact
      901 attcggcgtt attacttcaa gtaagagatg gtcaacaatg ggatcagcct attttgcaag
      961 gcgcgctatc tagcattgga gagttcggac tggttaaact gatccccgtg tggcagtcac
     1021 agctttcagt gacagagttt caagaggatg tgaccgccga taattacggc gaaccgctga
     1081 tgtatcaatt cagcgaatct gcctttggca aaaaaagcgt gtcacgaaat atcaaggttc
     1141 acgcaagtcg tgtaattttg ctcaatgagg gtggcgactt taattcccct cattctggag
     1201 ttcctctgct tgaggcgggt tacaacaagc tagttgatct cgaaaaaaca tcgggcggca
     1261 gcgccgaagg ttttctgaaa aacgcaagcc gacaattagg gatcaaatta acaaaagatg
     1321 ttgatttgag acagctcgaa gacagcgcaa aagcccttgg gttttcttca ttttctgatg
     1381 cgttgaacga caagatcaag aaaatcaact caggaacaga ctcagccttg attactcacg
     1441 aaggtgatgc ctctgtgttg tcagtcgcac cagcagatcc gaagccaacg tgggaaattt
     1501 ctgcaaatga gtttgccgcc tcagtacaaa tccctttcac gattttgttc ggacagcaaa
     1561 cagggcgatt ggcaagtgac gaggacaaaa ccgactgggc aaaccgttgc aacggtcgtc
     1621 gaaatggttt tctcaccgat gtgataacac aactacttaa tcgcttgtgg ttcatcggtg
     1681 tgctacctat gccaaaaaat agcgatgtca cagtgtcttg gtctgactta cttgcaccaa
     1741 gcgaaaaaga gaaaatcgca aacgcacagg ctttggcttc agttgcaact acatcgcagt
     1801 ctgcctttgg ttttgccgcg gtaacagcga acgaaatccg cgaatcttta gggtttgaac
     1861 ctctgcctga taatctgatt ccgccaacaa ttgacgacga aaatgaagat caagacgaaa
     1921 atcaagccac tgcggattcc gacaaataaa gccgatccgc tcaatatggg agtgtcggtt
     1981 gccaagctat ttcgtcacat tgatgtcatt tactcacaaa ttaagcgctc cattaagcaa
     2041 caaatcttgc aaaagatgtc actgcgactg caagcaaata attttgagta ttttccttcg
     2101 tatcttactg cgactgagtt ggcggatttg cttgaaacct tgcaaaagat tatcgacaaa
     2161 gagattttgt cgaatggttc tcaaggcgaa aatctctggt ttgactccta tatcgacgaa
     2221 gcagcactaa aaggcactca gtctgcggtc actgatttag gtctgcaatc agaggcttat
     2281 cgcagtcagc gaaacttatc atcaatcgtt ttcagtcctg cgtacttcaa ccgattggcg
     2341 attgcacaca tggcaagtta tagcgagtgg caaggtttaa gcgacgagtt gcgaaaatct
     2401 ttggcggggg tgatcacgga ggcggtttta aacggcaaaa acgtgaaaga aactgcccgt
     2461 gagattcggc aaaaacttga tgtgtcatca aaacgggcga agcgaatagc gcaatcaagc
     2521 cagttggcgg catatcgtcg ggcagaatgg caagaggcgg aagaggcaaa agaagaactt
     2581 gggctaaaca ccaagctctt acatttttca gcgctcaaag ctactacgcg attagcacac
     2641 gcccaacgcc acggcaaata cttcgatgtt gcagaggtgc gcgaatggta tagccgagac
     2701 ggcaaccaat tcaactgtta ctgcaagcag tctgtggttg tggtcaacgc cgacggtaaa
     2761 acagatattg agcctctcct tgttgggttg gctaaagaac gtaaacaatg ggtaggagca
     2821 ttaaaaaatg tcaaaaaata acgtgaacat cgtcaccgtc attaacagca aaaatatcag
     2881 cacagaatct attgacggag atcaacacct gattatccgc ggcgttgtgc cgattgttga
     2941 tgatgttgtg atgaacgacg gactttatcc tgcggatgaa atcaataaaa gctacaacag
     3001 cttagagggc aactttatgc cgcttgggca tccaaaaatc gaaggtaaat acgtttcagc
     3061 ccaagatgtt cgagcggtga acaaacatca cgtcggtgcg tgggcgaaga acgtacgcaa
     3121 ggaaagtggc aaagtgctgg tcgatatgta tatcaaccgc cgctttgcgg aaggttcaga
     3181 acagggcaaa accttgcttg aacgacttga cggaatggtg acaaacacca acgctgaacc
     3241 tattcacgtt tccacagggc tgattctaaa caaagtcaag caatcgggta aatccaaagg
     3301 caagaaatat agctggattg cgacaaatat gtcgttcgat catattgcaa tattgcttga
     3361 tgtgccgggc gcggcgacac ctgatgatgg cgtggggatt tttgtaaacc aagacggaag
     3421 cgaaatgggg gtggaaaatg taaacctaac acaagcggcg gattaccgca cagaaaagct
     3481 gtttgacaaa gtacgatttt acctgatggc gaactcagat ttatcgtttg aagaaattca
     3541 tcgcgcgctg cgtgaagcta ttcgccccga aggcaaagat aagtggtcat tttgggtcga
     3601 agctgtttac ccgaaatact tcatctattc ggacgataac agcggcaaga aatacaagcg
     3661 gagctatttt attgacgaaa atgacaatgt gcagctcact agcgaacctg tcgaagttgt
     3721 caagaagatt ggctacgaag aaattacaac caatgaggaa cataatccaa tgaaaaccaa
     3781 aatcttagcc gtgttgaatg cggcaaacgt gaaaacggaa ggcttaaatg acgatcagct
     3841 cttggaagcc tacaacaagc tgcaagcgga taagggtaaa gagcctgaaa agcctaaaaa
     3901 tgaagatgac agcgaaattg atgagaaaat caagaaagca gtcaatgccg cgattgagcc
     3961 actccagcaa gccttgcaag ctaatactga caaagagcaa gcagcaatgc gtgcggcggt
     4021 gaaagccaag ttcaatatga gcgatgccgc agtcaatgct ttgcaaggcg aggtattaac
     4081 tgagttgtat gcgcaaacgc aacaatcggt cggattaaac aataattcgg caaacggcaa
     4141 cggcgacaat caatgggatg gttactcact caaccaagag gagaaaggca atgagtaatg
     4201 taatttatcg cggttcggtc aagcgtgaac ctcaaacggt gaatatcgtg atcaacgata
     4261 cttcggcacc gggtgcagta gttaaactcc acaacggcaa attagaggcg gcggctgatt
     4321 taaaaggtcg ccgtttttta ttgggcaaca accgctttaa ggggcagaca atcgaacaag
     4381 cgtatgcgaa gggcgatact gcaacggcgt ttcgtttaga gcctgaacag gagtattacg
     4441 cgcaaattgc cgacggcact tataactttg gcgatgaact cacggtaaaa caagcgggcg
     4501 gcaaactgac caaagcggag acgggcgatg tagtgctatt tttcttcgac gaagaatcgc
     4561 agcgtgtaat cagtggcggc aaaggctatg ccgatgtcgt ggtggcaaac gcctacacta
     4621 aataagaggt aacaaagcaa tgttgaaatt cacaaaagaa caagaacgct tcgtgattaa
     4681 cgagcggatc aattacgaca aaaaacacgc gctaatggca gcaaattcgg cggggttatt
     4741 gggcaacgcg atgactttgc cgaaagatgt ctgggcggaa tgggatcgtg atgcagtgca
     4801 aattcagcgc gaagaattgg tggtgtttaa cgatttagcg ggcatttcaa aatcaatgcc
     4861 gattggcaaa cttattcacc atttccaaac ggtatctgat agcggttcgg ttaatatctc
     4921 attagacggt cgttcatcag cgaaaaccga taacgtggtg cttaactacc acggtacacc
     4981 gttgccgatt attgattcca ctttctcttt tggctggcgc gatatgtcgg cagctcaaag
     5041 cgaagggtat cagatcgacg gtgcagcacg cgcgaacagt ctccgcaagg tggctgaaaa
     5101 aatggaagat ctcgctttaa atggtgattc ctctattgtg gttggcaatg ccaagcttta
     5161 cggcttgcgt actgcgccaa atcgtatgac taacacacac aatctggatt tagccactgc
     5221 gacaccgaaa gaaatttacg atgtgttccg tgacttgatt agcaaattcc acgcgaagaa
     5281 ctactactct cctgtaacgc tttatgtgaa ctatggcgac tatttcgcaa tgagtacacg
     5341 tgattactcc gagcagaaat ccgaaggatc tatcttgaac aaagtgatga cgattccgca
     5401 aattgcgaaa atcgtgccag caagccgtgt accgcaaaat gaaattctcg gcttgtgcaa
     5461 gcgttcggat gtgtaccaag tgcttaacgg tatgccgtta gtgactcgtc cgattgcgcg
     5521 ccataatgaa accgatgatt actcgttcca aattatggca gccgttgccg tggagttcaa
     5581 attcgacgat aaaggcaatg ctggctatat ccagtacact aaatcctaag gcggtgcaaa
     5641 atgaagtggc tattaacaca cgatagccac gagttgaaga aaggcgatgt ttacgaaggc
     5701 gaaacattac cgctttggct tgtgggcaaa gctatcgaaa tcgaagaaaa atcctttgaa
     5761 gttgcaacgc cttctgatca cgctgacggc gaaactggcg aaactggcga aactggcgaa
     5821 actggcgaaa ctggcgaaac tggcgaaact ggcgaaactg gcgaaactgg tcagaaaggc
     5881 aaaaaagata aaggcaaata actatggcag cagaaataga gctatccgaa gcgcgaacaa
     5941 cgcttgacga actgggtttc acttcgccga actctctgct tgagctttac gttcagcaaa
     6001 cgaacacctt agaccaggcg ttgaacaaat cggggtacag cgaagaaaca cagaaactga
     6061 tcaagctcta tctggttgct attcttgcaa tttcatctgg ggcgcgccgt ataaagtcag
     6121 aaggtgcgcc tagcggtgca agccgaacgt ttgagtatga tccaaacgcg ctagacaacc
     6181 ttaagtcggc gattcgctca attgatccga aaggttgcgc tgaaagtttg ctacccaaag
     6241 ataaagttgt cggctttttc gatgtggtcg gaggctgcta atgtcaaatt tatcgaattg
     6301 gtcctacacc gcaaaagcca cgctttggca ggctaaagga aagaacgatg acggcgtttt
     6361 agttttttct acgccacaaa tcatttcctg tgactacggt gcagatagaa agcgcgcacg
     6421 ttttgagatt gggcgtgaac aagcggtcaa aaatgtgatc tggacggagt tcgcccacgc
     6481 caaattaggc gattatgttc tgattggcga aagcgagcaa gccgatccga ttttggctgg
     6541 cgcagaggaa attattcata tacagcgctt tgctgatacg ttcgaccgca atcgtgatga
     6601 ttttgcgctg ataacaggcg ggtaaaatgg ggatcaaagt aaaaggcata agacaggtca
     6661 agcagcggct gaatcaggtt gttggcgagg ttcagagcca aaaagcaact cgggcaatgt
     6721 ttcgcatttt gaatgcggtt gcccctcttg cggcgcacta tacgcctgtt gatacctcaa
     6781 cgttgatcaa cagtcaattc actgaaatgg aagtgaacgg cacacgcttg attggaagag
     6841 tgggttattc tgctaattat gcggcttacg ttcacgatcc taatatcaag caaaacttcc
     6901 gcaagccttc agcgaagaaa gagtttttga cttccgcgct gaacgaatca aaggctacga
     6961 tcaaatccat tgttaaagag gagttgtcac tatgatttct tatgtcaaag ccttcaaaca
     7021 atggttagag gctcactctc ttgcagacgg ctatgttgtg cagctttacc agtgggagga
     7081 tacgcaaaaa gccaagcctg ttattgttat tcagccaaat agtggttcgc cgcaagtttc
     7141 tgacctgagc agcgaacact atctgctaat tagccttgtt gcaggtaaaa attcaggtta
     7201 cacaatcgaa gagcgagcaa gggcgattat gtcaaagatt ttagttgagc ctttcgcttc
     7261 ctttggttac atcgaatcaa tgggcggatt acccgcccca attttcaccg aagataatcg
     7321 aatgattttt cgcttaccac ttcggattat ttcaacaaat caagaggagt agtttatggg
     7381 ttctccatct ccacaaaata acgcagaaaa tctcgtggtg gggcgtgccg tagttttaga
     7441 gtacggtacg actgatgcaa agccacaaga aagcgaatgg aaggttgcgg gcgcaatgac
     7501 gacaaagagt tgggatttta gcccaaattc ggttacatca gaggctgatg atgctggcgg
     7561 cttccctgaa accttaatta caaactcaga ctttagtatt tctggtgacg gtgagtggcg
     7621 caaacgacca aaatcgaaag aactaggcat caaagatcta gttgtgttat acgcaaatgc
     7681 ggtaaaaaat cgcacccaac cttacatttg ggttcgtttg aaatacgcag gcttaacaat
     7741 tctcggaaaa atggttatca ctgcattaag tagcgaagcc ccaaccaatg acttagtgaa
     7801 attctctata gagttcaagg ttggcgattc aagcactatc gaaattgctg cagcgtaaaa
     7861 aatcccaccc tctataacaa gagcggtggg attatcatta tcctagggat agttgtaggg
     7921 gctttcctaa gctagccaaa atacgaccaa tagtgtcgat ttttgtaata tggcgagggg
     7981 ctaaaattcg ctgtacttct ggcggacgga tattagttaa tcgggcaatt tctgccttgc
     8041 ttaccgcttg ttcttgcatt gtattatgca aaagcacttt tgcgtagatg ctctctggca
     8101 taaacacagg cacttcgtcg gtatttactg ggcgagtgag cggaaagcgt ctatcttcat
     8161 cgaaataaat ttcaacacaa gatagtagca cgtcttctgc catttccatt gcttcttcaa
     8221 atgtatcgcc ttgggtgata gcttctggca aatcaggaaa tgtcactaca aagccccctt
     8281 cttcagcggg ggtaaataac gctggataaa acatattttc tccttattca agggtgtagt
     8341 gaaagcccct taacatcagg ggctttatat tattttaagc ctagttgctt tttaacccct
     8401 tccactaagc ctgtttttaa ctcttggctt gggtgtttgg ggaggtggct tcttttgccc
     8461 ttgtagtaga gtttcaaatg ctttgatcca ttttcaacct ctaccccatt agcctttagc
     8521 caccttagga attcgctttg cttcactacc tcctcctatt tgtttaactt gggttcatta
     8581 tatgcaaaaa tgctaacttt gtcaacagaa agttagcaaa aatgctaatt tttttgttga
     8641 ggtgaaaatg gttagctatc caattactga aattggtgaa atgcttattg caaccgatgg
     8701 gcgagacttt ttattcagac cgagtttcaa aaatatctac aaaattggaa gcccacaaga
     8761 aattgtaaaa atctatgcac tattgcacgg ctgtgagatt cgagagatat tgccgcacat
     8821 tttaaccaaa tcggagtctc atcaatctta cttttggtca gtgctgaatt cgcctgtttt
     8881 tggcagaaaa attctgcaaa acgcaatgat cgtgatgtcg tgctgttgcg atgatgatat
     8941 ttccctgttg attggcgagt ggttgccgtc aaagtctggc gttaggtaca agttgggtaa
     9001 aatgccacct gagacaatta tcaattttgc aaaaaattta atgggtcacg gagtgattgg
     9061 ttgctgcgaa ttgccaaaga aagaacaatc tgcaaaaaat gaattttcag aaagttttga
     9121 tgctgttgag tacataagtt tagcccgaac tcatttcaat atgagccgcg aagacgctga
     9181 aaatctcacg atgaccgaat tgcagcaact tctaaaaagt caaatgcctg ccgaaaataa
     9241 tagctatgga cagttcaccg aagaagagta cgacagaatt atggcggaat atgagaaaag
     9301 aagagcggct taaatagccg ctaacattta aaagaaccta cctgatagaa cgggagtgaa
     9361 atccttgtaa tgtgctgaat aaaaaagctc tcttatgtca ttgagtagct caaattcatc
     9421 tttggatctt gcgttgcaga ttgccacata gtcatctacg caggataaaa tacgttcaat
     9481 actgacttct tttatccagc atacttcttg gttttcattc agccagttgg caaagtcttg
     9541 tttagtgcag tcttttttca taagttctcc tttttgatta tttgcaaaaa aaatcatatc
     9601 aaatcatccg ttgttatcta ttggcgaaaa taatttttgc gacacagatc gcaaaaatag
     9661 gttttgacgg tggaaaaacg aatcttaatt cgtagaatgg taaaatttct ttatttttaa
     9721 ggtattttta tgaaaaaaat gattgttttt gtagcttctg ttttgtcatc ttttgttatt
     9781 gcatcggatt gggaaataat agaagcggct caaaataagg tgaaagcaga gcttataaga
     9841 aaatcagaaa aaaaagcaga gttatgttta aatgttgctc aggctgtctt aattactaat
     9901 caaaaagtac aaaaagatac tttctcaatg tgtggtgaat tttcgacaga gattgtcttt
     9961 tctgactcta aagcatatag ccttgcagta tgtggaaagg ttaaaggtaa agatattatc
    10021 ggaaatccga tagagagcga ctatatatac caaagagcaa ctaaggattt ttactttaat
    10081 tataatggca aaaaatcagc ttattatgat cctcaatctg atcttgcaag gcttcaagag
    10141 gtctcag
//
LOCUS       DANLUQ010000085        10121 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.085, whole genome shotgun sequence.
ACCESSION   DANLUQ010000085 DANLUQ010000000
VERSION     DANLUQ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10121
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.085"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            177..1637
                     /gene="xseA"
                     /locus_tag="PXB27_001631"
     CDS             177..1637
                     /gene="xseA"
                     /locus_tag="PXB27_001631"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDL5346402.1"
                     /translation="MSNILTVTQLNYSVRHLLEMEMSHIWLTGEISNFSQPVSGHWYL
                     TLKDDKAQVRCAMFKMKNVRVNFRPQNGMQVLVRASVTLYEPRGDYQLIIESMQPAGE
                     GLLQQQFEQLKAKLSAQGLFAQEHKKPIPTFVKTVGIITSSSGAALQDILHILQRRDP
                     SLSVVIYPALVQGKEATQDIVNMIELANRRNECDVLIVGRGGGSLEDLWCFNEESVAY
                     AIYHSQIPIISAVGHETDVTIADFVADLRAPTPSAAAELVSRDQNELKRQLQHQFDKV
                     NLAFDRVWNHKFNRFQQLPLRLNAQHPSKQIQSQQQRFDYMEYRLEQAIYALVAAKKQ
                     VGKELNVRLIHQHPQKQIERQSQMLKQLELKLHWQMEQQLSKKQQKWKNITQRFERNP
                     LPYQLYQQQTMVAKMSQRLAYSIEKIMLKEQRHFQELCTKLDGLSPLKILTRGYSITQ
                     TKDGKTLTSTENIERGEAIITQLKTGKIISQVVDFL"
     gene            1949..2034
                     /locus_tag="PXB27_001632"
     tRNA            1949..2034
                     /locus_tag="PXB27_001632"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1983..1985,aa:Leu,seq:caa)
     gene            2132..2956
                     /locus_tag="PXB27_001633"
     CDS             2132..2956
                     /locus_tag="PXB27_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL5346403.1"
                     /translation="MKPLQNGTNKPFTITIASTKGGSAKSTNAANIGAFCAEHGLRTL
                     LIDTDTQPTLSSYYELEYQAPGGTYEFLHFKDVDPTHIISRTTIPNLDLIQSNDPSNK
                     ISPMLRDSPDGALRFSLLLQKIEGYDVIIIDTRGTRDITVDMSVLAADVLFCPILPHI
                     LSAKEFIRGTIGMYQDLETFTAFGFTLPPLKAMINCVDHTKDVKLVSDYLHTLFQNEF
                     DENKTLLEFSVPDRVAYREAATFSKPVYRQSQAEYETISQLCALLMPQFANSHFSA"
     gene            2968..4329
                     /gene="dnaB"
                     /locus_tag="PXB27_001634"
     CDS             2968..4329
                     /gene="dnaB"
                     /locus_tag="PXB27_001634"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDL5346404.1"
                     /translation="MSSLNKSLVQPHSIEAEFSVIGGLLLDNELFDEVSSKINEGDFY
                     NFTHRLLFKHISELIEAGKPADILTLDQYLTQKKVIEDLGGFAYLAEIVKNTPSTANI
                     HAYTDIVLLYSKQRQLLKLGQFIVDQTQSAKTPDKFEAVVDDIEKKITEFSLSDNTKS
                     AADLSDIFANMLSRMELSAKNGDPVTGTPTGIQGIDEATTGGQPGDLIVIGARPSMGK
                     TAFSQTIAYHTLKKFESAPVFYHSMEMPADQILQRFLAMRARVGLQNIRQADSLDDEE
                     WSKLTSAMGYILEHWKNRLVIDDEGSLTPQKLRTKVRQNARKYGKPAAIFIDYIQLMQ
                     TSQRYENRNLEIANISRSLKLLAKEMDCPVYALSQLNRSVEQRANKRPINSDLRESGS
                     LEQDADVILFIYRDEVYDADSPFKGTAEIIIGKQRNGPLTSVMTNFRSEFALFENRTN
                     TTH"
     gene            4338..5984
                     /locus_tag="PXB27_001635"
     CDS             4338..5984
                     /locus_tag="PXB27_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HDL5346405.1"
                     /translation="MTKDEKRLAAIAKGLNVSPISNTAPSYQTATPTHYSSDVEYITV
                     TLDKLRPYEHNPRKTRNPNFEMIKESIRRRGLDHKPNITRRPGEDFYIIADGGNTRIQ
                     ALKELFTETKDPKFWSISCQYKPWQGDTADSVEAELNLLIGHLIENDTRADLSFIEKA
                     LGILQAKEYYEKKLGKSLSSRELSSELENDGYIISHALILKMERCVNYLYPHIPEVLF
                     SGLGHTQIDKLLAIRNNAEEVWAAHQFDTDVTFESLWSENLSKFNEATPFQAKEFQSE
                     LITAMVEALEGKVSFETLYLDIDLDEQKFKKIAAKQQEIDNLVEKTVDQINTRQEQQT
                     AKSEPLIKNENKAVTPQKAQHIIDEVIDDNESDDEQNNAITDLSQLGSSLLDNQDNQE
                     DYLSQIAHNFGLTPGMSIQEQREQRAEANGLTFACEGRQPVEDIWLIHPARQYRSEAY
                     SLALDIAESMGIDHLVEHIVKQPVDYTYAMKPADTELSPSALTLYSFLSMLQTAEHKD
                     ENSCVLDSKFLFELDDLTLVKIFRLIRVSRHIRQQGGEND"
     gene            5977..6534
                     /locus_tag="PXB27_001636"
     CDS             5977..6534
                     /locus_tag="PXB27_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2857 domain-containing protein"
                     /protein_id="HDL5346406.1"
                     /translation="MIKPITVNEALIGNALLNIKEGNIHPCLKMGFSEEQLKAINNLS
                     IDEIMDISNSVVSFAKVEINHETFWKLLAIAQANTQQRQIIDRALLLGASIEMLHQYF
                     GLSTSEVSARRQLLGIEEKMGRKAAASDEESTHIWEIWQRYKQRMEILDSQEGLELLC
                     LIAEEGNMNLTVVWKLVSEWYSKHK"
     gene            6693..7883
                     /locus_tag="PXB27_001637"
     CDS             6693..7883
                     /locus_tag="PXB27_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346407.1"
                     /translation="MFSSTNKPTEHGLLFFGNQHETVPTRLLQDPCLTPRAKFAWQLI
                     KCNVQAFQGGLFPSYETLSKMLSDRPYAEAKLSEKVVTQTLYLLRLTRWLTLCETVRD
                     ERGYVQGNVYILHDEPVPILDAIQLNSDYLSFLDKCTKHKDSLINGVANHIVESLITD
                     KTKWHYVSHLDCLQSRYQIYQQQQLVKDKQPSQTEQELSKIQQNILTSTMEVRDFDEE
                     VRKKSSKNQTSNKGVRAKNEDKSLILDLLPLGKSDVQYRTLYINKYCTGEPSEITWPQ
                     EIPLTSLEQKLTAQAMKGLDLGLCQAILLEVQQRIIQKQDVKKPKGYLYTLIQRAKQG
                     EFKPYYFELGKNLPSQNQANNNSAHSIQKLTEQPTEKPVLTSADREQMASRMQKFRAE
                     LLLQ"
     gene            8062..8820
                     /locus_tag="PXB27_001638"
     CDS             8062..8820
                     /locus_tag="PXB27_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03761 family integrating conjugative element
                     protein"
                     /protein_id="HDL5346408.1"
                     /translation="MTTQLDPQLGPLRSEITFTLHTQYAHKLWVGRPMIRNEEGKVTQ
                     SSIISVPNCFAMLTQIQRAASEDDPYADDYLIQFEESVINYRKEIQKLTNDIVTLYAK
                     MLPEGISIERCANISPISYPIYVNSQLGYQLLYLLGDFDSLCRSVMTAAHIAIINRAE
                     AQDWIEAGARLIRKCFGIVERYKSSGITRRDYQEKNARYQAAIKRMGYTLSDAVLTGE
                     HRAEFAPFIKQNTTVEEEQSVETHITAQTNESQK"
     gene            8829..9314
                     /locus_tag="PXB27_001639"
     CDS             8829..9314
                     /locus_tag="PXB27_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3158 family protein"
                     /protein_id="HDL5346409.1"
                     /translation="MNLISDEIYRQLVKDSGLNTSLKQLFSHFDTGSDYELLQEQFTQ
                     ARAYFMAAQDSMVQSVRQDLSPLAVYMIKDKASSSGGTFLRWRSMQNARTGGTVWQPI
                     VDDKSVPVEVRKKVVAVEKDRILINMQISVFNHILRQLADCAEKLKEVDNAVAKSDLN
                     S"
     gene            9670..>10121
                     /gene="ssb"
                     /locus_tag="PXB27_001640"
     CDS             9670..>10121
                     /gene="ssb"
                     /locus_tag="PXB27_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL5346410.1"
                     /translation="MAGINKVIIVGNLGNDPEMRTMPNGEAVANISVATSESWTDKNT
                     GERREVTEWHRIVFYRRYAEICGQYLRKGSKIYIEGKLRTRKWQDQNGQERYTTEIQG
                     ELLQMLDSRNATNSVDGQQSNYQAMSQSPKATHPQDLPMDNFDDDIPF"
BASE COUNT         3294 a         1990 c         2028 g         2809 t
ORIGIN      
        1 tggttcaaat ccggcccccg caaccacatt ccaaaccctc gatagtgaat cacatcgagg
       61 gttttcgttt tatttgccaa aaaacagcaa aaaataaccg cttgttgact gcaaaagctt
      121 cctctcgctt tcatttctcg ttacacttct actacattta cgcttttttg attattatga
      181 gcaatatttt aactgttacc caactcaact attccgtccg ccatttgctc gaaatggaaa
      241 tgagccatat ttggctaacc ggcgaaatct caaatttcag ccaaccagtt tcggggcatt
      301 ggtatctcac tttaaaagat gacaaagcac aagtgcgttg tgcgatgttt aaaatgaaaa
      361 atgtaagagt gaattttcgc ccacaaaacg gtatgcaggt gctggtgcgg gcaagtgtta
      421 cactctatga gccacgaggt gactatcagc tcattattga gagtatgcaa ccggcgggcg
      481 aaggcttgtt acagcagcaa tttgagcaac ttaaagcaaa actttcagct caaggcttgt
      541 ttgctcaaga gcataaaaag ccaattccta cttttgttaa aaccgttggt attattacct
      601 cctcaagcgg agccgcatta caagatattt tacatatttt gcaacgacga gatccgagct
      661 tatcagtggt tatttacccg gctctcgtgc aaggcaaaga agcaacgcaa gatattgtga
      721 atatgattga actggcaaac cgccgcaatg agtgtgatgt attaattgtc gggcgaggtg
      781 gcggttcatt agaggattta tggtgtttca acgaagagag tgtcgcttat gccatttatc
      841 attcacaaat cccgattatc agtgcggtgg gacacgaaac cgatgtgacc attgctgatt
      901 ttgttgcaga tttgcgcgca ccaacgcctt cggctgctgc ggaattagtc agcagagatc
      961 aaaatgaatt gaaacgccaa ttacaacatc agtttgataa agtgaattta gcctttgacc
     1021 gagtttggaa ccataaattt aaccgtttcc aacaactccc cttacgcctg aatgctcagc
     1081 atccaagcaa acaaattcaa tcgcaacagc aacgttttga ttatatggaa taccgcctcg
     1141 agcaggcaat ttatgcactt gttgcagcca aaaagcaggt ggggaaagag ctaaatgtcc
     1201 gtttaattca ccaacatccg caaaagcaga ttgaacgcca atcccaaatg cttaagcaat
     1261 tggagctgaa actccattgg caaatggagc agcaactcag caaaaaacag caaaaatgga
     1321 aaaacattac gcaacggttt gaacgcaacc cgctacctta tcaactttat cagcaacaaa
     1381 cgatggtggc aaaaatgtcg caacgcttgg cttattctat cgaaaaaata atgctgaaag
     1441 agcagcgaca ttttcaagag ttatgcacta aacttgatgg acttagccca ttgaaaatct
     1501 tgaccagagg ctactcaatt acccaaacta aagacggtaa aacgcttact tcaacagaaa
     1561 atattgaacg tggtgaggct attatcaccc aactaaaaac aggaaaaata ataagtcaag
     1621 tagtggattt tttgtagggg aaattttgaa cgctgccaat ttttaaaatt atttatagta
     1681 aaatcagtag gttaaatatt tataaacagg gttgttcact tagttatcca caggtgatgt
     1741 gcataactat ttttgtaaag atgaattgag tgctgatttt taaatcaacc gatttcaaaa
     1801 ggcattttta cgcttgcaca agcggtcata tttccctata atcgtgcaaa aattaagcca
     1861 gtgtggcgaa atcggtagac gcagcggatt caaaatctta tgcgtaaact gataggacat
     1921 acaggcgatt gaataaaaag cactcttagc ctgagtgatg aaattggtag acatgacgga
     1981 ttcaaaatcc gttggtgaat aaccgtgtcg gttcgagtcc gacctcaggc accatttgaa
     2041 tcacacttgt agtttctctc tttcaaataa tcaattcaaa tacaccgtat taatggccaa
     2101 tactccttaa cccttgttaa gagagtaaat tatgaaacca ttacaaaacg gtacaaacaa
     2161 accttttacc attactattg cttcaactaa aggtggctct gccaagagta cgaatgctgc
     2221 taacattggt gcattctgtg cagaacacgg tctaagaacc cttcttattg ataccgatac
     2281 ccaacccaca ttaagctctt attatgagtt ggaatatcaa gctcccggtg gaacttacga
     2341 atttctgcat tttaaagatg tagatcctac ccacattatt tctagaacga ctatcccaaa
     2401 tcttgacttg atccagtcta atgacccgtc aaataaaatc agcccaatgc tgagagattc
     2461 acctgacggt gcattacgtt ttagtttact gctccagaaa attgaaggtt acgatgtcat
     2521 cattattgac actcgtggta ccagagatat tactgtcgat atgtctgtat tggcagcaga
     2581 tgttttattc tgtcctattc ttccccacat tttatctgct aaggagttta ttcgtggcac
     2641 gattggaatg tatcaagacc tcgaaacctt tacggcattt ggctttaccc taccaccgct
     2701 gaaagcgatg attaactgtg ttgatcacac taaagatgtc aaattggttt ccgactacct
     2761 tcatactctc ttccaaaatg agtttgatga aaacaaaaca ttgctggaat tttcggtacc
     2821 tgaccgcgtg gcatatcgtg aagcggcaac tttttcaaaa ccggtctatc gtcagagcca
     2881 agctgaatat gaaacgatca gtcagctttg tgcgttactg atgccacagt ttgcaaactc
     2941 tcacttctca gcatagaggg agaatgtatg tctagcttaa ataaatcact tgtacagccg
     3001 cattctatag aggctgaatt ttcagtaatc ggtggattac tcttggataa tgaactcttt
     3061 gatgaagtga gtagcaaaat caatgagggg gatttttata attttactca ccgtctactg
     3121 tttaagcata tttccgaatt gattgaagcc ggtaaaccag cagatatatt gacattggat
     3181 caatacctta cccagaaaaa agtgatcgag gatttgggcg gatttgctta tcttgcagaa
     3241 atagtcaaaa acactccatc tacagctaat atccacgctt atacggatat cgttctctta
     3301 tatagtaaac agcgacagct tttaaaactg gggcagttta ttgttgatca gacacagagt
     3361 gcgaaaacgc cagataagtt tgaagctgtt gttgatgaca ttgagaaaaa aattactgag
     3421 tttagcctat cagataatac aaaatctgca gcagatttaa gtgatatttt tgccaatatg
     3481 ctctctcgta tggaactctc ggctaaaaat ggagatcctg tgacaggtac tccaactggt
     3541 attcaaggta ttgacgaagc gacaacaggt ggacagccag gcgatttaat cgtgattggt
     3601 gcccgccctt ctatgggaaa aaccgcattt tctcaaacta tcgcttacca tacacttaaa
     3661 aaattcgagt cggctcctgt gttctatcac agcatggaaa tgccggcaga tcagatttta
     3721 caacgttttc tagctatgcg tgccagagtg ggattgcaaa atattagaca ggcagacagt
     3781 ttggatgatg aggaatggtc aaaactaacc tcagcaatgg gctatatcct agaacattgg
     3841 aaaaatcgtt tagttattga tgatgaaggt agtctcacgc ctcaaaaatt acgcactaaa
     3901 gttcgacaaa atgctcgtaa atatggcaaa ccggcagcta ttttcattga ttacattcag
     3961 ttaatgcaaa cctcgcaacg ttatgaaaat cgcaatttag aaattgccaa catctctcgt
     4021 tcactcaaat tactggcaaa agagatggat tgtccggttt atgccctctc tcagctgaac
     4081 cggtctgtgg aacaacgtgc caataaacgc ccgatcaact ctgatttacg ggaatccggc
     4141 tcacttgagc aagatgccga tgtcatttta ttcatctacc gagatgaagt ttatgatgcg
     4201 gattctccgt ttaaaggcac tgctgaaatc attatcggca agcaaagaaa cggaccactt
     4261 acttccgtaa tgacgaattt cagaagtgaa tttgccctat ttgaaaacag aacaaacaca
     4321 acgcattagg atagattatg acaaaagatg aaaaaagact ggcagcgata gcaaaaggat
     4381 taaatgtttc acctatttca aatacggctc catcctacca aacagccaca ccaacgcatt
     4441 acagtagcga tgttgaatat attacagtaa cgctggataa gttgcgtccg tatgagcata
     4501 accctcggaa aacccgtaac cctaactttg agatgatcaa agagtctatt cgtcgtcgag
     4561 ggttagatca taagccgaat atcacccgtc gtccgggaga agatttctat attatcgctg
     4621 acggtggtaa tactcgtatt caggcactca aggagctctt taccgagacc aaagatccaa
     4681 aattttggtc catcagttgt caatataaac cttggcaagg tgatactgcc gacagtgtcg
     4741 aagctgaact caatttactt atcgggcatt tgattgaaaa tgatacccgt gcggatttgt
     4801 catttattga aaaagcattg gggattttgc aggcgaagga gtattacgag aaaaaattag
     4861 gtaagtctct ttcttcgaga gagttatctt ctgagttaga aaatgatgga tatattatct
     4921 ctcatgctct cattcttaaa atggaacgtt gtgttaatta cttatatcca catataccag
     4981 aagttctttt tagtggtctt ggtcatacac agatcgataa actattagct attcgtaata
     5041 atgctgaaga ggtatgggca gcccatcaat ttgatactga tgttactttt gaatcgctct
     5101 ggtcagaaaa tttatccaaa tttaatgagg ctacaccatt tcaagcaaaa gaattccaaa
     5161 gtgaattgat tacggcaatg gttgaagctt tagagggtaa agtgagtttt gaaacacttt
     5221 atctggatat tgacttagat gaacaaaaat tcaaaaaaat agcggctaaa caacaagaaa
     5281 ttgataattt agttgaaaaa acagtcgatc aaatcaatac cagacaggaa caacaaactg
     5341 caaaatctga acctctcatc aaaaatgaaa ataaagccgt tactccccaa aaagctcaac
     5401 atattattga tgaggtaatt gatgataacg aaagtgatga tgagcagaat aacgctatta
     5461 ctgatttatc tcagcttggc tcttcattgc tcgataacca agataatcaa gaagattatc
     5521 tatcccaaat tgcccataac tttggactca ctccgggaat gagtattcaa gaacagcgag
     5581 agcaacgagc tgaagcgaat ggactgactt ttgcttgtga aggtcgccaa ccggttgaag
     5641 atatttggtt gattcacccc gcacgtcaat atcgctcaga ggcctactca ctggccttag
     5701 atattgccga aagtatgggc attgaccatc tggttgaaca tattgtgaaa caaccagtgg
     5761 attacactta tgcaatgaaa ccggcagata ctgaactttc accgtctgct ttaacactct
     5821 actcattcct ctcaatgtta cagactgctg aacataaaga tgaaaatagc tgtgtcttag
     5881 attccaaatt cctgtttgag cttgatgatc ttaccttagt caaaattttc cgtctcattc
     5941 gggtcagtcg ccatattcgc caacaaggag gagaaaatga ttaagccgat taccgtaaat
     6001 gaagcattga ttggtaacgc cctgctcaat attaaagagg gaaatatcca tccttgtcta
     6061 aaaatgggat tttctgagga gcaactaaaa gcgatcaata atctcagtat tgatgaaatt
     6121 atggatatta gcaactccgt tgtctctttt gctaaggtgg agattaatca cgaaaccttt
     6181 tggaaattac tggctattgc acaagccaat actcaacagc gacaaattat tgaccgagca
     6241 ttattgctcg gagcctctat tgaaatgttg caccagtatt ttggtttatc aacctctgaa
     6301 gtctcggctc gccgtcaatt actcggtatc gaagaaaaaa tgggacgaaa agcggcagca
     6361 agtgatgaag aatctaccca tatttgggaa atttggcaaa gatacaaaca acgaatggaa
     6421 atcctagatt cacaagaagg attagagctt ctatgtctga ttgctgagga aggcaatatg
     6481 aatctaacgg ttgtttggaa actggtttcc gaatggtatt caaaacataa gtaaaaaata
     6541 aatgccccga agttggagct ttggggcatt cgatattgag tcttgtatct ctatcttatg
     6601 aattacaagg actttgcaga agtcccgtgc attatagcca taggagagag aatagcaaga
     6661 gaaaaactct tttatcctgg tggagagaaa caatgtttag cagcacgaat aagccaacag
     6721 agcatggatt gctatttttt ggcaaccaac acgaaacagt ccctactcga ttattacaag
     6781 atccctgcct tacaccaaga gcaaagtttg cttggcagct cattaagtgc aatgttcaag
     6841 cctttcaagg aggattattt ccctcttatg agacgctcag caaaatgtta tccgatagac
     6901 cttatgcgga ggctaaatta tcggaaaaag tcgtgactca aacactctat ttactccgtc
     6961 ttactcgctg gcttacttta tgtgaaacag tgcgtgatga gcgaggttat gtacaaggta
     7021 atgtttatat cctacatgat gaacctgtgc caattttaga tgcaattcaa ttaaatagcg
     7081 actatctctc ttttttagat aaatgcacca aacataaaga tagccttatt aatggtgtgg
     7141 caaatcatat tgttgaaagt ctaataacag acaaaaccaa atggcactat gtctctcatt
     7201 tagactgttt acaaagtcgt tatcagatat accagcaaca gcagctggtg aaagataaac
     7261 agccatcgca aacagaacaa gaattaagca aaattcaaca aaacatccta acttccacta
     7321 tggaagttag ggattttgat gaggaagtca ggaaaaaatc ctctaaaaac cagacttcca
     7381 ataagggagt tagggctaaa aatgaagata agtcattgat tttagactta cttccattag
     7441 ggaagtcaga tgtacagtac cgtactttat atattaataa gtactgtact ggggaaccga
     7501 gcgaaattac ttggccacaa gaaatcccat taacttcact tgaacaaaaa cttactgcac
     7561 aggcaatgaa gggattagac ctcggtttat gccaagctat tttgttggaa gtacaacaac
     7621 gcattattca aaagcaggat gtgaaaaaac cgaaaggtta tctctatacg cttatccagc
     7681 gggcgaagca gggtgaattt aaaccctact attttgaatt aggaaaaaat cttccctcac
     7741 agaatcaggc taacaataat tctgctcatt ctatacaaaa actgactgag cagcctactg
     7801 agaaaccggt cttaacttca gcagacagag agcaaatggc tagtcgtatg cagaagttta
     7861 gagctgaatt acttttacaa taagattaaa aaacaatcac tgttaagggg cttaatagtg
     7921 agtgaaaaat aatcagtgtg taccaggtac acaatggata aaaaataaac agcgtaggca
     7981 gtgtctgcaa agattaaaaa acagacaatg gaaagcccct ttccaaagac taaaaaataa
     8041 tcaaataaac aaggagcata tatgaccact caattagatc ctcaacttgg tccacttcgt
     8101 agtgagatca cttttacgtt acatacccaa tatgcccata aattatgggt ggggcgacca
     8161 atgattcgta atgaggaagg caaagtaact caatcctcca ttatcagcgt accaaattgc
     8221 tttgcaatgc taacccaaat tcaacgggct gcatcagaag atgatcctta tgctgacgat
     8281 tatctgattc aatttgaaga aagtgtgatc aattatcgga aggaaatcca aaagctcacc
     8341 aacgacattg tcacgctcta cgccaaaatg ttgccggagg gaatttctat tgaacgctgt
     8401 gccaatattt ccccgatttc ttacccgatt tatgttaatt cacagctagg gtatcaatta
     8461 ctctacttac ttggtgattt tgatagcctt tgccgttcag taatgacggc agctcatatt
     8521 gcgattatca atcgtgcaga agctcaagat tggattgaag ctggtgcaag gttaatccgg
     8581 aaatgttttg ggatcgttga gcgttataag agttcaggaa tcactcgtcg ggactatcaa
     8641 gaaaagaatg ctcgctatca agctgcgata aaacgaatgg gctatacact ctcagatgct
     8701 gtacttaccg gtgagcatcg tgctgagttt gctcccttca tcaagcaaaa cactactgtt
     8761 gaagaagagc aatccgtaga aacccatatc acggcacaga caaatgaatc tcaaaaataa
     8821 ggagtgatat gaacctcatc tctgatgaaa tttatcggca attagtcaaa gattctgggc
     8881 tgaatacttc tctgaaacag ctatttagcc attttgacac tggctcagac tatgaacttt
     8941 tgcaagaaca gttcactcaa gctcgggctt attttatggc tgcacaggat agtatggtgc
     9001 agtctgtgcg gcaggatctt tcacctttgg ctgtttatat gattaaggat aaggcctcat
     9061 cctcaggcgg tacattttta cgctggcgta gtatgcaaaa tgccagaacc ggtggcacag
     9121 tttggcagcc gattgtagat gataagtctg tcccagtgga ggtgaggaaa aaagttgtcg
     9181 cagtagaaaa agacaggatt ttgattaata tgcagatttc ggtgtttaat catattttgc
     9241 gacagttggc agattgtgct gaaaagttga aagaggtaga taatgccgtt gcgaaatcag
     9301 acttaaattc gtagtaaatt tatatataac aactcctata ttggttgtgt gtaaacgcca
     9361 accacggtgg gctgacgtgg taaaacaaac agtccgttaa ttaggacata ttcaagtttg
     9421 taatacctga aaatccggtt tgtaatccct gaaaaagtgc agtttgtaaa tcctgcatta
     9481 aaaggccttt taaaggtaag ggtacaagcc ccttaccttc acgaagaaaa agcctttaca
     9541 acggaggaat aagtgattgc acacgtaaaa atgaaaaatt cactctaacc aaattttaat
     9601 taaatacttt tttcttaggc tgaatcaagt ttaatagaac taacttaatt tattgaagga
     9661 gtaaatacga tggcaggaat caataaagtg attattgtcg gtaatcttgg caacgatcct
     9721 gaaatgcgga ctatgcctaa tggtgaagca gttgctaata ttagtgtggc aacttctgaa
     9781 agttggacgg ataaaaatac cggagaacgt cgagaagtca ctgaatggca tcgtatcgtt
     9841 ttttatcgcc gttatgctga aatttgtggt cagtacctac gtaaaggctc taaaatttat
     9901 atcgaaggaa aattacggac acgtaagtgg caagaccaaa acgggcaaga gcgttataca
     9961 accgaaatcc aaggtgaact attacaaatg ttagatagcc gtaatgctac aaatagcgta
    10021 gatggacaac aatctaacta tcaagctatg agccaatctc caaaagcaac acatccacaa
    10081 gatttaccaa tggataactt tgatgatgat attccgttct g
//
LOCUS       DANLUQ010000086        10066 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.086, whole genome shotgun sequence.
ACCESSION   DANLUQ010000086 DANLUQ010000000
VERSION     DANLUQ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 10066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10066
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.086"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            54..995
                     /gene="argC"
                     /locus_tag="PXB27_001641"
     CDS             54..995
                     /gene="argC"
                     /locus_tag="PXB27_001641"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDL5346411.1"
                     /translation="MSKYKIFVDGAVGTTGLRIHERLSQADDIELLTLPEEQRKDLNA
                     RVAKVAEADLTFLCLPDEASKELVANAPENTRICDTSTAHRVNPNWVYGFPELSGQRE
                     KIQNAKRVAVPGCHATGYIALVRPLVEKGILPADYPLSCHSLTGYSGGGKAMIADYEN
                     ENSPNAFNAPRVYGLTLQHKHLPEMQAVTQMQNPPIFTPVVADYYSGMLVTVPLQIFA
                     LQAVKSGEEIANLLADYYQTSRLIHVHPFQSLPEDGMLAANAYAGKDSLEIFIYGNST
                     QLLLAARFDNLGKGASGAAVQCMNIMLGRDEVEGLEL"
     gene            1087..1941
                     /gene="argB"
                     /locus_tag="PXB27_001642"
     CDS             1087..1941
                     /gene="argB"
                     /locus_tag="PXB27_001642"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDL5346412.1"
                     /translation="MQQQEINQFANLLEKSTACLVKWQGKIIVVKYGGNAMINEELKK
                     SVMADLLLLHRLGVKVVLVHGGGLEISQGVKLLGKEPQFINGLRVTDSDTINVVLQML
                     AGKVNKSLVALLKGKGIGLCGIDGAMLQCKKLEAEVDYGFVGDIVKVDPTVIEIALNA
                     GLIPVVSTVGVDENGQAYNINADTAASEIAIALQAEKLVSMTDIAGLLRDRFDESTLI
                     PEVEVSEVQSLIEQGIIAGGMIPKIACCTDFIRAGGSEANIIDGRIDHAILVEMFGGK
                     NGTRFYKK"
     gene            1954..3138
                     /locus_tag="PXB27_001643"
     CDS             1954..3138
                     /locus_tag="PXB27_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HDL5346413.1"
                     /translation="MTSSNTIKQLDKDYIAQTYGRFDLALSHGKGCEVWDFEGNKYLD
                     FTSGIGVNSLGWADEDWLQAVTTQAGKLAHTSNLFFTEPASQLAQKLVAASGLKRAFF
                     CNSGAEANEGAIKTARKYSFDKYGSGRSTVLTLVNSFHGRTISTLASTGQEVFHKHFF
                     PFTEGFEYLPVNGIEALKARLETENVCGILLEVVQGEGGVCPLEADYLHAVQSLCQEK
                     DILFMIDEVQTGIGRTGTLFAYQQFDLTPGLVTLAKGLGGGLPIGAFLLGEKVEMTLG
                     KSDHGSTFGGNPVSCAAANVVIDKINAEFLAQVQQKGQLLQQRLASLPKVKSVSGLGL
                     MLGVEFEEGILAADIVAKCINKGVLFLTAKSKLRLLPPLMISEQQIEQGIRVLEDVLK
                     DS"
     gene            3321..3752
                     /locus_tag="PXB27_001644"
     CDS             3321..3752
                     /locus_tag="PXB27_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL5346414.1"
                     /translation="MSNQIALLLMSHGSFARAALASAELIVGQQENVAAIGVVAVDNV
                     ADIKRQMEQTIAELNTDKGLIILTDIVGGTPMNLASSQLTHPNVFACSGLNLPLLLEV
                     LMSRDGNLQEIRENLPLAYQNGFSLRTQQDFVSQEDDDDLL"
     gene            3739..4206
                     /locus_tag="PXB27_001645"
     CDS             3739..4206
                     /locus_tag="PXB27_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL5346415.1"
                     /translation="MICFSRIDDRLIHGQVVTTWVNLHRIEQIIVLNDAVAADPTQKQ
                     ILTMAAPQGIKVVAFGIEKFGEVLQKTQITRRTMLLFTNSIDVLRTVELGVSIDKLNV
                     GGMRYQARREQLSKAVSVTPEERQAFKTLLEKGVEVTVQMVPNDEKLNLQEII"
     gene            4209..4976
                     /locus_tag="PXB27_001646"
     CDS             4209..4976
                     /locus_tag="PXB27_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL5346416.1"
                     /translation="MLTALLVACWAGICALDDIGTQMIRRPLLIAPVVGLIMGDLETA
                     LIIGATLEVMWMGIGNVGAYSAPDMISGTAIGTALGIAAGEASTAVALAVPTSLLAQQ
                     LLILYRSGVCALNPIAERWAASGDFDKIFRIHYIPMLIAFSIRAIPTFLAVYYGADII
                     AQAVSALPAKVITGLNVAGKIIPAIGIGLLMLMMIKKAELWTFLIAGFTLAVYLKLSV
                     LPITLIALPLALIYDLAVSKKAEAAPTKTADEEDYDL"
     gene            4973..5779
                     /locus_tag="PXB27_001647"
     CDS             4973..5779
                     /locus_tag="PXB27_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL5346417.1"
                     /translation="MSNKITKQDLNKVFWRMQVLNVTNNFQSMQAIGFLSSFIPVLKR
                     LYKDRPQEERAEAMKRHLKFFNSHVNSDALILGIAAAVEETTKEDEKETVTAIKTGLM
                     GPLAGLGDSLLKFTWLPICGSIGAALALSGNVLGPILMFLLYNSVNIATKYYGIHLGY
                     SKGVDLIHGAGNNILQRLGNMANVVGLMVIGALIASVVKVKVVSQIAAGDNVIKFQEM
                     LDKVMPGLLSLLLTVVIYWILKKTNGKYAATMILATMGICIGLVYAGILG"
     gene            5779..6816
                     /locus_tag="PXB27_001648"
     CDS             5779..6816
                     /locus_tag="PXB27_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HDL5346418.1"
                     /translation="MDSVYDIIRLEQGRYLFRQQPAQSVQIFFLDNPDSKDSKWLAET
                     DLAEFELKLSACEARPFFLIQSANGEQIVAERTLPVAGMNNFRDMGGYVAHQGKRVKW
                     GKLYRSDHLHNLRDEGVAYLDKLGIQTVIDYRSPNEVAKYPNPPINGREQTFRLDPNA
                     HTAELAAQFSADKHDEDRNLVNKIIAQKAEGNLINRYDIVMAQYRNFVEKAECQTAFA
                     EMLRLATDPENAPLVQHCRGGKDRTGFGAMLLLGILGVSKADIIADYMLTHYNRLARN
                     EEKMAIYRTFTQDQDVLDYLLSLIDTQPEFIEQSLNTIETQYGTIEQYAQRVLGITAK
                     EIEALRANYLA"
     gene            6906..8126
                     /gene="agp"
                     /locus_tag="PXB27_001649"
                     /pseudo
     CDS             6906..8126
                     /gene="agp"
                     /locus_tag="PXB27_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005628146.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
     gene            8434..>10066
                     /locus_tag="PXB27_001650"
     CDS             8434..>10066
                     /locus_tag="PXB27_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDL5346419.1"
                     /translation="MSQDIQADLKKILALLSRNRRFQSLFLLLLRSEQFINSDALSTE
                     LQLSVRTVKKEIKLLKEKLEPYGIQIFSRPFYGYKLLIQFEELHTQLKQYFQISQPMT
                     VNNKFESRVNAILRRLLVSQRALKVEDFQSELYLSFTSALTKEFSQVKALLGKYGLIL
                     EAKPYHGMNIVGESYRKIMLTVRLYKYFTQLEHPFGIKEFEQCFACPMTEKIRERLAK
                     TIIHFNLVFSDIYAERFIIYLRYFYNQQPELLLPKLNFNYQQTNEYQFVIKLLEQLRE
                     QDSRFEFNPEVIRFLTYIAIASTDLYRFKDCNSDNYPLLLDKAWEIHRFIFTQFSAYL
                     NLSEIHDDTCRKDLLKMLIPIAMKMMLSISDDVDLGLFNQTELHRNPILAHTVEKLVD
                     DIEKNYGYSLSIREQYFIFDIFSGMVNRIELERKPLRLAIIALNGRLSTQQLKRNIKR
                     YFSDYILKIETKVLYELAIMEDKPYDYYLCNEYGKNMAIPYKPIYFADSSLNENEYAT
                     SLNEVFYHAYQYDEVLPSIKSIEIEAQYRLNEFHLE"
BASE COUNT         3046 a         1883 c         2240 g         2897 t
ORIGIN      
        1 agccttaaat agccttagac taccgagcag aaaaagtcaa agaaggattt tcaatgagca
       61 aatacaagat ttttgttgat ggggcagtag gaacaaccgg gctacgcatt cacgaacgtt
      121 taagccaagc ggacgatatt gaattattaa ccttgcccga agagcagcgg aaagatttga
      181 acgcccgtgt ggcaaaagtc gcagaggcgg atttaacttt cctctgtttg ccggatgaag
      241 cctcaaaaga attagtagca aatgcaccgg aaaatacccg aatttgcgat acttcaaccg
      301 ctcatcgggt caatcctaat tgggtttatg gttttccgga attgagcgga caacgtgaaa
      361 aaatccaaaa tgccaagcgt gttgcggtgc cgggttgcca tgccacgggc tatattgcgt
      421 tggttcgtcc actggtggaa aaagggattc tgcccgccga ttacccttta agttgtcatt
      481 ccttaaccgg ctattcaggc ggtggcaaag cgatgattgc tgactatgaa aatgaaaaca
      541 gcccaaatgc tttcaatgcc cctcgtgttt acggtttaac tctccaacac aaacatttgc
      601 ctgaaatgca ggcagttacc caaatgcaaa acccaccgat tttcacccct gtggttgctg
      661 attattacag cggaatgtta gtcactgtgc cattgcagat cttcgcctta caagcggtca
      721 aatccggcga agaaattgca aatttgctcg ccgattacta ccaaaccagc cgactaattc
      781 acgttcaccc tttccaatct ctacccgaag acggaatgct tgccgcgaat gcttatgcag
      841 gtaaagacag cttagagatt tttatttacg gcaacagcac ccaactttta cttgctgccc
      901 gttttgacaa tttaggcaaa ggggcatcag gtgccgcagt gcagtgtatg aatattatgt
      961 tgggaagaga tgaggttgag gggttagagc tttaaatata aatgtaggga caggtcctgt
     1021 gcctgtccga taatcatcgc ccatattcgg acgggcacag gacccgtccc tacaagagaa
     1081 agaaatatgc aacaacaaga aatcaatcaa tttgcaaatt tattagaaaa atccaccgct
     1141 tgtttggtga aatggcaggg taaaatcatt gtggtgaaat atggcggtaa cgcaatgatt
     1201 aatgaggaac tgaaaaaatc agtgatggca gatctgctgt tacttcatcg tttaggcgta
     1261 aaagtggtac tggtacacgg tggcgggctg gagatttcac aaggcgtgaa attattgggt
     1321 aaagaaccgc aatttatcaa cgggttgcgt gttaccgata gcgatacgat taacgtggtt
     1381 ttgcaaatgt tggcaggcaa agttaataaa agccttgtcg ccttattaaa agggaaaggc
     1441 attgggctgt gtggtattga tggggcgatg ttgcaatgca aaaaattaga ggcagaggtg
     1501 gattacggct ttgtgggcga tattgtgaaa gtcgatccaa ctgtgattga aattgctctt
     1561 aatgcgggct tgattccggt tgtttccacg gtgggcgttg atgaaaacgg gcaggcttat
     1621 aacattaatg cagacaccgc tgccagcgag attgcgattg cgttacaagc ggaaaaattg
     1681 gtctcgatga ccgatattgc aggtttattg cgagatcgct ttgatgaaag caccttaatt
     1741 ccggaagtgg aagtaagcga agtgcagtcc ctgattgagc aaggcattat tgccggtggg
     1801 atgatcccca aaattgcgtg ctgtaccgat ttcatcagag ctggtggctc tgaggcaaat
     1861 attattgacg ggcgaatcga ccacgccatt ttggtagaaa tgttcggtgg caaaaacggc
     1921 acacggtttt ataaaaaata acaggaaagt gaaatgacaa gcagtaatac gattaagcaa
     1981 ttagataagg attatatcgc ccaaacctat ggcagatttg accttgcact gtcgcacgga
     2041 aaaggctgtg aagtgtggga ttttgagggc aataagtatc ttgattttac cagtggcatt
     2101 ggggtaaata gtttaggctg ggcggatgag gattggctac aagctgttac cacacaagcc
     2161 ggcaaattag ctcacacctc aaacctgttt tttaccgaac cggcaagcca attagcccaa
     2221 aaattagtgg ctgcaagcgg tctaaaacgg gcattttttt gcaattcagg ggcagaggca
     2281 aatgaaggag caattaaaac cgcccgcaaa tatagtttcg acaaatacgg ttccggtcgt
     2341 tcaacggttt taaccctcgt gaactctttt cacggacgaa ccatttcaac cctcgcatcc
     2401 actgggcaag aggtattcca caagcatttt ttccccttta cagagggctt tgaatattta
     2461 cctgtaaatg gcattgaggc actgaaagct cgcttggaaa ctgagaacgt ttgcgggatt
     2521 ttgctggagg tggtgcaggg cgagggcggt gtttgcccat tagaggcgga ttatctgcac
     2581 gccgtgcaaa gcctgtgcca agaaaaagat attttattta tgattgatga ggtgcaaacc
     2641 ggcattgggc gaacgggcac actttttgct tatcaacaat ttgatttaac tcctggttta
     2701 gtcacgctcg ctaaaggctt gggcggcggc ttgccgattg gtgctttcct gttaggcgaa
     2761 aaagtggaaa tgacattagg caaaagcgac cacggctcaa cctttggtgg caatcctgtt
     2821 tcctgtgctg ctgcaaatgt agtaatcgat aaaataaacg ccgaatttct cgcccaagtg
     2881 caacaaaaag ggcagttgtt acagcaacgc ttagcgagct taccaaaagt taaatccgtt
     2941 tcaggcttag gtttaatgct cggcgtagaa tttgaggaag gcattttagc cgctgatatt
     3001 gttgcaaaat gtatcaacaa aggtgtgctg tttttaaccg ctaaaagcaa gttaaggcta
     3061 ctcccaccgc tgatgatctc tgaacaacag atagaacaag gaatcagagt gttagaggat
     3121 gtcttgaaag attcttgagt tattataaac agtaggaata tcttcttact gttttttatt
     3181 actattatat agtagcctac tttaatctgt tgtcacttta tatttcttct tatgtgtacg
     3241 atatatcgtg caataacttc ccttgtttat ctgtataaca ttttatttaa tagaatgact
     3301 tataaatcaa ggagtgactt atgtctaatc aaattgcgtt attgttgatg agccacggca
     3361 gttttgcgag ggcggcgttg gcgagtgccg agctgattgt cgggcagcag gaaaatgtgg
     3421 cagctatcgg cgtagtagca gtggataatg tagcggacat taaacggcag atggagcaaa
     3481 ccattgctga gttaaacacc gacaaaggct tgattattct caccgatatt gttggtggta
     3541 cgccgatgaa tcttgcctct tctcaattaa cccaccctaa tgtttttgcc tgttcgggtt
     3601 tgaatttacc tttattgcta gaagtgctga tgagccgtga cggcaatttg caagaaattc
     3661 gggaaaatct accgcttgca taccaaaacg gatttagcct acgcacacaa caagattttg
     3721 tttcacagga ggacgatgat gatctgcttt agccgtattg atgaccgttt aattcacggt
     3781 caagtagtca ccacttgggt taatttacac cgtattgaac aaattattgt gttaaatgat
     3841 gcggtggcag cggatccaac ccaaaaacag attttaacga tggcagcacc gcagggaatt
     3901 aaagtggtcg cgtttggcat tgaaaaattt ggtgaagtgt tgcagaagac acaaattacc
     3961 cgccgtacta tgttgttgtt taccaatagc attgatgtgt tacgcactgt agaactaggt
     4021 gtatcgattg acaaattgaa tgtcggcgga atgcgttatc aagctaggcg tgagcagtta
     4081 agtaaagcgg tttcagttac gccagaagag cgtcaagcat ttaaaacctt attggaaaag
     4141 ggtgttgaag taacggtaca gatggtaccg aatgatgaaa aacttaactt acaggagatt
     4201 atctagctat gctgactgca ctacttgttg cctgctgggc aggtatttgt gccttagatg
     4261 atattggcac acagatgatc cgccgtccgt tgttaatcgc accggtggtc ggattgatta
     4321 tgggggattt ggaaacggcg ttgattattg gtgctacgct tgaagtgatg tggatgggga
     4381 tcggcaatgt tggtgcatat tctgcaccgg atatgatttc cggaaccgct attggcaccg
     4441 cacttggtat tgctgctggt gaagcttcta ctgcggttgc actcgcagta ccgacctcac
     4501 tcttggcaca gcagttactc attctttacc gctccggcgt atgtgcattg aacccaattg
     4561 ctgaacgctg ggcggcaagc ggtgattttg acaagatttt ccgcattcac tatatcccaa
     4621 tgttaattgc cttttctatt cgtgctattc caacgttctt agccgtctat tacggggcgg
     4681 acattattgc ccaagcggtc tctgctctgc cggcaaaagt gattaccgga ttaaatgttg
     4741 caggcaaaat tattcctgcc atcggtatcg gtttgctgat gttaatgatg attaagaaag
     4801 ccgaactttg gacgttctta attgctggct ttacgttggc ggtttatctc aaattatcag
     4861 tattgccgat taccttaatt gcactaccgc ttgcgttgat ttacgactta gcagtaagca
     4921 aaaaagctga agctgcacca accaaaaccg cagatgaaga ggattatgac ctatgagcaa
     4981 taaaattacc aaacaagatt taaacaaagt gttttggcgg atgcaggtgc tgaacgtcac
     5041 aaacaacttc caatcgatgc aggcgattgg atttttaagt agctttattc cggtattaaa
     5101 acgactttat aaagatcgtc cacaggaaga acgagcggaa gcaatgaaac gccacttaaa
     5161 attttttaac agccacgtta attctgatgc attgatttta ggaattgccg ctgcggtaga
     5221 agagactacc aaagaggatg aaaaagagac agtaaccgcg attaaaaccg gtttaatggg
     5281 gccgttagca gggctgggag acagcttgtt gaaattcacc tggttaccga tttgcggtag
     5341 tatcggggcg gcattggcac taagtggcaa tgtgttaggc ccgattttaa tgttcttgtt
     5401 gtataacagt gtaaacattg cgacgaagta ttacggtatc catctaggtt acagcaaagg
     5461 ggttgattta attcacggtg cgggtaataa cattttgcaa cgactcggca atatggcaaa
     5521 tgtggttggt ttaatggtga ttggcgcatt aattgcctcc gttgtgaaag tcaaagtagt
     5581 ttcacaaatt gccgctggtg ataatgtgat taaattccaa gagatgttag ataaagtgat
     5641 gccgggacta ctcagcttac ttttaactgt cgtgatctac tggattttga agaaaactaa
     5701 cggcaaatat gctgcaacga tgatccttgc tacgatgggg atctgtatcg gcttagtcta
     5761 tgcaggaata ctcggctaat ggacagtgtg tatgacatta ttcggttgga acagggcaga
     5821 tacctgttcc gccaacagcc ggctcaatcg gtacagattt tctttctgga caaccctgac
     5881 agtaaggata gcaaatggct ggcggagact gatttggcag agtttgagct gaaattgagc
     5941 gcttgtgaag cccgtccgtt cttcctcatt caatcagcaa acggagaaca gattgttgct
     6001 gaacgcacct tacctgttgc cggaatgaat aattttcggg atatgggggg ctatgtcgct
     6061 catcagggca agcgagtgaa atggggcaag ttataccgct ccgatcatct gcataattta
     6121 agagacgaag gcgtggctta tcttgataaa ttgggaattc agactgttat tgactatcgc
     6181 agcccgaatg aagtcgccaa atacccaaat cccccaatta atggcagaga gcaaactttt
     6241 cggcttgatc cgaatgcaca tactgccgag cttgctgccc aatttagtgc agacaagcac
     6301 gatgaagatc gtaatttggt caataaaatc attgctcaaa aagctgaagg taatcttatc
     6361 aatcgctacg acattgtaat ggcacaatat cgtaattttg tcgaaaaagc agaatgccaa
     6421 accgcctttg ctgaaatgtt aaggttggcg acagaccctg aaaatgcacc attggttcaa
     6481 cactgccgtg gagggaaaga tcgcactggt ttcggtgcaa tgttactgct tggtatttta
     6541 ggcgtgagta aagctgatat tattgctgat tatatgctta ctcattataa ccgccttgct
     6601 cgtaacgaag agaaaatggc gatttaccgt accttcactc aagatcaaga tgtgttggac
     6661 tacctattgt ctttaatcga tactcaaccc gaatttatcg aacaatcgct taatactatt
     6721 gaaacccaat atggtactat tgagcaatat gcccaaaggg tattaggtat tactgcgaag
     6781 gaaattgaag cattacgggc aaattatttg gcgtagaacg gtagggtgta atattgaaca
     6841 cactacaaat ggagaaattt catatgcgtt attttgcaaa acattggtat aaaactaccg
     6901 cttgtctgac aacaattttc acagcatttc ctctttttgc tcaaccaatt gaaaatatac
     6961 caacttccca ctacgaatta gaaaaaatca ttatatttag ccgacacggg ttacgttccc
     7021 ctcttacaga aaaaggcagt gatttagaaa aatcaacccc ttataaatgg gcaaattgga
     7081 gtgtaccgac cggtcattta acccataaag ggacgctgtt ggaaacctat tttgggcagt
     7141 atcttggtca atggctcaca cataatgggt tattggacaa ggcaagctgt gaaagtggga
     7201 agcagattga ggtttatgct aatagcctgc aacgtacaat tggcactgca caagctttgg
     7261 ttgccggtgg gttttcaggt tgtaatgtac aagttaaaca ccaccagcag attggaaaga
     7321 tggatccggt gtttaatcca attattcgct ccgatgacca gacatttgtg caaacagcat
     7381 taagcaaaat tgacctcaat aaaacccatc agtatttagc gccatcctat catttattaa
     7441 gcgaaataat cgattatgct cattcagaaa aatgtttgca ggaaaaacag tgtgaatttt
     7501 ttaatgatgt tggtaaatta aggattaaaa aggataaata accaggtgtt tccggtacga
     7561 tgcgattaag taaaaatatt gtcgatgcct tattgctgca atattatgaa ggttttcctt
     7621 taaaagagat tgcaaataac cagcttgata gtgatcagaa atggcaaaca ctcaataaaa
     7681 ttaaaaacga ttaccaattt atgctacgag gagatccatt tattgctaaa catatttcat
     7741 taccgctatt aaaatatatt cagcaagatt taaatagtga aaataaaata acacttttag
     7801 tcggacacga ttccaatatt atagccttac tttctgcatt aaatgttaag ccttataaat
     7861 tagcacatca atatgaacaa actccaatag gagggaaagt ctttattgaa atctggaaag
     7921 aaaaaagtag ccaagcacaa aaagttaaac tggaatatgt atatcaatct actgaccaaa
     7981 ttcgtaaagc gattccacta agccttgata aaccaccgca acatcacatt ttgagtttgg
     8041 attattgcca agctgaccaa tatggattat gcgattatat ggaatttaaa cagttactgg
     8101 aaaaagtcat tgctgataca ttgtgagatt ttgacaaagt gagtgaaaaa ggtagcataa
     8161 aaaagtcggg acagttgcca gctgctccct gtaagatgtt gtttacagca ctagtaatgc
     8221 tactatcccc aaaaagctgc tgaaaggcag cttttttgtt gtatataaaa gtacaccttt
     8281 ctacgcttca ttctttttct ccaattaaac ctaatttagg acttctagtg ataaattcgg
     8341 tatcatatct taattcacta catatcgtgc aagccttaaa attgaaaggg ctgacgaaat
     8401 cctatttaat tctgacacac ataagggaat attatgtcgc aagatattca agccgattta
     8461 aaaaagatat tggcactatt aagccgtaac cgtcgatttc aatcattatt tttattactg
     8521 ttaagaagtg aacaatttat taatagtgat gcactttcta cggagttaca gctttcagta
     8581 cgaacggtaa aaaaagagat taagttatta aaagaaaaac ttgaacctta cggcattcag
     8641 attttttctc gaccgtttta tggctataaa ttattgattc agtttgaaga attacacacg
     8701 caactaaaac agtatttcca aatatcacag ccgatgacgg taaataataa atttgagtct
     8761 cgggtaaatg cgattttacg ccgattactg gtgagccagc gtgcattgaa agttgaagat
     8821 tttcagagtg agctttattt aagttttacc agtgccttaa caaaagaatt tagtcaagtt
     8881 aaagccttgt taggaaaata tggattgata ctcgaagcga aaccctatca cggaatgaac
     8941 attgtaggtg aaagttatcg taaaataatg ctcacagtac gattatataa atattttacc
     9001 cagcttgaac atccatttgg aattaaagaa tttgaacagt gttttgcttg tcctatgact
     9061 gaaaaaatcc gtgaaaggtt agctaagaca attattcatt tcaatttagt attttctgat
     9121 atttatgctg agcggtttat tatttactta cgctattttt ataatcaaca gcctgagtta
     9181 ttgttaccga agttaaattt taattatcaa cagacgaatg aatatcagtt tgtaattaag
     9241 ttactggaac agcttcgtga acaagacagt cgttttgagt ttaatccgga agttattcga
     9301 tttctgacct atattgcgat agccagtaca gatctctacc gctttaaaga ttgtaattcg
     9361 gataattacc cattgttgct agataaggct tgggaaattc atcgttttat ttttactcag
     9421 ttttctgcat atttaaattt atcggaaatt cacgatgaca cttgccgtaa agatttgctt
     9481 aaaatgctga tccctatcgc aatgaaaatg atgcttagca tttcagatga tgtggattta
     9541 gggctattta accaaacaga actacataga aatccaattt tggctcatac agtagaaaaa
     9601 ttagtggatg acattgaaaa aaactacggg tatagcctat ctattcgaga acagtatttt
     9661 atttttgata ttttttccgg aatggttaat cgaattgaat tagaacgtaa accgctccga
     9721 ctggcgatta ttgcccttaa tggcagatta agcacccaac agttaaaacg gaatattaag
     9781 cggtactttt ccgactatat tttgaaaatt gaaaccaaag tgctatatga gcttgccata
     9841 atggaagata aaccttatga ctactatctc tgtaatgaat atggaaaaaa tatggctatt
     9901 ccctataagc ctatttattt tgctgacagt agtttaaatg aaaatgaata tgcaacatca
     9961 ctcaacgaag tattttatca cgcttatcaa tatgatgagg tgttgcctag cattaaatcg
    10021 attgaaattg aagcacaata tcgcctaaat gagtttcatc ttgagc
//
LOCUS       DANLUQ010000087         9959 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.087, whole genome shotgun sequence.
ACCESSION   DANLUQ010000087 DANLUQ010000000
VERSION     DANLUQ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9959
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.087"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(92..1114)
                     /locus_tag="PXB27_001651"
     CDS             complement(92..1114)
                     /locus_tag="PXB27_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL5346420.1"
                     /translation="MANGTSRNRRSSGRVTLADVAKVVGVGQMTVSRALRTPEMVSEP
                     LRKRIEEAIIELGYIPHFSARQLASAESHNLVIVTTSISSTENTLILSALRKSVADSA
                     LQMVILIADQESWFQELINHAPQAIVFLNLDCPPNVREWIKNSNIPTVEIGAIKKEPI
                     HLSVGIDSHEAMEKMIHFLVKKGYREIGLLSAKQDLSIFKQYLESWHTTLFAHHISPL
                     KIHHVVEDISFSAGATLLTEALLNWEAIDALVFLSDELACGALYEALRRHMSIPKDLA
                     IAGLGGLEVGSISYPKLTTIAIPYEQMGKIAGKQLLTLLSGKTLSAEEMIVKLPTKLV
                     IRDSAA"
     gene            1345..1614
                     /locus_tag="PXB27_001652"
     CDS             1345..1614
                     /locus_tag="PXB27_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005540356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL5346421.1"
                     /translation="MKIMAVCGSGLGSSFMMEMNIKKVLAKLGVEAEVSHTDLSSVVA
                     DDADLFVMAKDIAASSNVPKEKLIVVKNIVGLAEFEEKLTAYFNQ"
     gene            1627..2977
                     /locus_tag="PXB27_001653"
                     /pseudo
     CDS             1627..2977
                     /locus_tag="PXB27_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013745980.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
     gene            complement(3072..4220)
                     /gene="dprA"
                     /locus_tag="PXB27_001654"
     CDS             complement(3072..4220)
                     /gene="dprA"
                     /locus_tag="PXB27_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDL5346422.1"
                     /translation="MAYHYDLLRLLQVPQMGAQRIGRLLAELNFAEFCHYDKLQLKQI
                     GWNEKQIQRWFNPDQKWIDQAMTWSELPNQQIVTLFDENYPFLLKQISTAPPLIYVKG
                     NVESLSLPQIAIVGSRDYSHYGEYWAGQFAAELARHNIIITSGLAIGIDGFAHQKIVM
                     EQGITIAVLGSGLNQVYPARHKKLAEQIVDLGGALVSEFSPNQPPVAENFPRRNRIIS
                     GLSLGTLVIEATLNSGSLITARYALEQGREVFALPNAVQNPYAQGCHKLIKEGALLVE
                     GVEDILEVVASQYQPQQIPLFDESKPTVSTSLQAVKNSEKFAKNLPSLTACQQQIYQH
                     ISLEPMAIDDLAKVTELTIEILLVELLGLELAGVIKQVSGGYVMDNGY"
     gene            4309..4821
                     /gene="def"
                     /locus_tag="PXB27_001655"
     CDS             4309..4821
                     /gene="def"
                     /locus_tag="PXB27_001655"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005621146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDL5346423.1"
                     /translation="MAILDVVLYPDEKLATVCEPVEQVDEALNRFIDDMFETMYEHEG
                     IGLAAPQVGVLKRVITIDIEGDKTNQVVLINPEILESSGETGIEEGCLSIPGHRALVP
                     RKEKVKVKALNRQGEEIIIDADDLFAICIQHEIDHLNGVLFVDHISNLKRQRIKEKMQ
                     KLKKQIARAK"
     gene            4915..5841
                     /gene="rfaD"
                     /locus_tag="PXB27_001656"
     CDS             4915..5841
                     /gene="rfaD"
                     /locus_tag="PXB27_001656"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HDL5346424.1"
                     /translation="MIIVTGGAGMIGSNIVKALNEIGRKDILVVDNLKNGEKFINLVD
                     LDIADYCDKEDFIASIIAGDDFGEIDAVFHQGACSATTEWDGKYLMQNNYEYSKELLH
                     FCLDRQIPFFYASSAATYGGRSDNFIEKREFEGPLNAYGYSKFLFDEYVRRILPEAES
                     PVCGFKYFNVYGPREQHKGSMASVAFHLNNQMLKGENPKLFAGSEQFLRDFVYVEDVA
                     KVNLWAWQNSISGIFNLGTGKAESFQEVANAVIKYHGKGEIETIPFPDHLKSRYQTFT
                     QADLTNLRAAGYTGTFKTVAEGTAEYMAWLNR"
     gene            complement(5909..6439)
                     /gene="fabA"
                     /locus_tag="PXB27_001657"
     CDS             complement(5909..6439)
                     /gene="fabA"
                     /locus_tag="PXB27_001657"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017807287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HDL5346425.1"
                     /translation="MNSCTPIIKPSYTYEELLLSGKGELFGEKGPQLPAPPMLMMDRI
                     IEMNQEKGNFEKGYIEAEFDIKPDLWFFNCHFINDPVMPGCLGLDAMWQLVGFYLGWI
                     GGEGKGRALGVGEVKFTGQILPTAKKVTYRIHMKRVVNRKLVMGVADGEVEVDGRVIY
                     TATDLKVGLFQDTTAF"
     gene            complement(6552..8189)
                     /locus_tag="PXB27_001658"
     CDS             complement(6552..8189)
                     /locus_tag="PXB27_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL5346426.1"
                     /translation="MTLSLLSSQKLVMQNDFSRLDEKLNFLDFQPQAKQALNQFLQSQ
                     FGALLVLKSELFPEFINEITEYLKKYSVDLPIISKMDFNKNSLFGYSVFLERELKTEY
                     VKGAIHEADKGILIVNIRSLLLDITQWDKLKQALLFGEYEPNSVNHSPYLSAKEKSTF
                     KLILVGEREDISTLSAYDDGLYQFGQYTEIKSYLSLSDNVEQWGGYVQNYAENLINKR
                     LTSTALNQFLQAYIRESESRELVSISPTLLRKHLLGIQNFYPNVTEIDEVKGYFDYLE
                     QQSSILNQYTVGDILTNQLYIETEGEEIGQINGLSVIEFEGIPHSFGEPLRMSCNVQY
                     GDGEITDIERKVELGGNIHSKGIIIAQSCLANLLELPTQLPFSASIAFEQSYGEIDGD
                     SSSLAIFNVLVSALAKVGLPQSIAVTGAIDQFGNVLSVGGVNQKIEGFFNICHARQLT
                     GKQGVIIPAACVSHLSLSSEVVEAVEQGLFKIWTVENVFDAIAILFERDFYDDEKAPN
                     SAQTALFTLIHQNLDERESEETSGSFLSKICKKIKLD"
     gene            8282..8728
                     /gene="matP"
                     /locus_tag="PXB27_001659"
     CDS             8282..8728
                     /gene="matP"
                     /locus_tag="PXB27_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HDL5346427.1"
                     /translation="MRYQKLITQETTWKWQYLLKKHREGENITKYEEQSLIDLKVQFL
                     STLQNSPDEVEKWIKSEMTPEQRKKMRQSVRAKRKRFFNAEKQTTKKKSIDLEYSSWL
                     RLSKYAKSQKLTLSEAIVKLTDELENKQLYLEQVAKMKSSLKDLLK"
     gene            complement(8794..9930)
                     /gene="ompA"
                     /locus_tag="PXB27_001660"
     CDS             complement(8794..9930)
                     /gene="ompA"
                     /locus_tag="PXB27_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_119863613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HDL5346428.1"
                     /translation="MKKTLVALAVLSAAAVAQAAPQANTFYAGAKAGWASFHDGLTQF
                     DNTYDYGSGINLGINLGINRNSVTYGVFGGYQILNQNNFGLATELGYDYFGRVRGNTT
                     FSGDDKEKTAAKHSAHGAHLSLKPSYEVVPNLDVYGKVGVALVRNDYYVQQHVDKDSR
                     IKVHNLKPSLLLGAGLEYAITPELAARVEYQYLNRVGNLDKAVRTTVGYLIPRGTNFQ
                     YSPDIHSVSAGLSYRFGQGAAPVEAPEVVTKNFAFSSDVLFDFGKSSLKPAAATSLDA
                     AHAEISNLGLANLAIQVNGYTDRIGKEASNLKLSQRRAETVANYIVSKGTNPANVTAV
                     GYGEANPVTGHTCDAVKGRKALIACLAPDRRVEIQVQGSKEVTM"
BASE COUNT         3000 a         2062 c         1863 g         3034 t
ORIGIN      
        1 aaaaaatcag aaattatttt aaagcgttaa attctatcat tttctacaag acttgccacg
       61 atcttttata gatttttatg caaaatcaat attatgcggc gctatcacga atcactagct
      121 tagtcggtaa tttaacaatc atctcttctg ctgataaggt tttaccactc aaaagagtaa
      181 gtagttgttt acctgctatt tttcccattt gctcgtaagg gatcgcaatg gttgtcaatt
      241 tagggtagga aatactcccc acttctaaac cacctaaacc ggcaatcgca agatcttttg
      301 gaatgctcat atgtcggcgt aatgcttcat ataatgctcc gcaagccaat tcatcggata
      361 aaaaaaccaa tgcatctatt gcttcccaat ttagaagggc ttcagtcaac aaagttgctc
      421 ctgccgaaaa cgaaatatcc tctactacgt ggtggatttt taaaggagaa atatgatgtg
      481 caaataaagt cgtatgccaa ctttctaaat attgtttgaa gatggataaa tcttgcttcg
      541 ccgataacaa tccaatctct cgatagcctt ttttcaccaa aaaatggatc attttctcca
      601 ttgcttcgtg gctatcaatt ccaacactga gatgaatcgg ttcttttttt atcgcaccta
      661 tttctacggt agggatattg ctgtttttaa tccactcacg gacattagga gggcaatcta
      721 aattcaaaaa cactatcgct tgaggagcat gattaatcag ttcttgaaac caagattctt
      781 gatcagcgat caaaatcacc atttgcaatg cggaatccgc cacgcttttg cgtaatgccg
      841 ataaaattaa ggtattttcc gttgagctaa tcgacgtcgt cacaatgacc aaattatgtg
      901 attctgcaga agctaattgc cgagcagaaa aatgcggaat atatcctagt tcaatgattg
      961 cctcttcaat ccgtttacgt agtggctccg acaccatttc tggtgtacgc aaagcacgag
     1021 ataccgtcat ctgcccaaca ccaacaactt tggctacatc agctaaagtg actctgcccg
     1081 aagatcttcg atttctactt gttccatttg ccatactatt ttcctaattg ttccatatta
     1141 tagggctaaa tccttaattt tcctatttag aaagaacaga gcaaaatgcc aactgattat
     1201 tttttatcta attaaatact aaaaatgtta acgctatcac atttttaata ttttctgttt
     1261 gatagcgtta acatttctat tctatagtat ttctatagtt caaaattcag acaactattc
     1321 attcaccttt aatggagatt tactatgaaa attatggcag tatgtggttc cggcttaggt
     1381 agcagcttta tgatggaaat gaacattaaa aaagtattgg caaaactcgg tgtcgaagcg
     1441 gaagtgagtc ataccgacct ttcctctgtt gttgctgatg atgctgattt atttgtgatg
     1501 gcaaaagata ttgccgcaag cagcaatgtg ccgaaagaaa aactcatcgt ggtaaaaaac
     1561 attgttggat tagcagaatt cgaagaaaaa ttgaccgctt actttaacca ataacggaga
     1621 tgttatatgg aatcgctact cttttttata ttagatattc tcaaagtgcc ttccgttcta
     1681 gtgggtttaa ttgctctggt cggtttagtt gcacagaaaa aatcggtatc cgatattgtt
     1741 aaaggaacaa tcaaaactat tttaggcttc ttagtgttaa gcggtggtgc aacagtactt
     1801 ttaagctccc tgactccttt aggcggtatg tttgaacacg ccttcaacgt gcaaggcatt
     1861 atccctaata acgaagcgat tgtgtctatg gcaatcgaaa aatatggtac ggcgactgcg
     1921 ttaattatgg cattcggtat ggtagccaat attttaattg cacgttttac ccgcctaaaa
     1981 ttcattttct taaccggaca ccataccttc tatatggcgt gtatgatcgg cgtaatccta
     2041 accgttgccg gctttgaagg tattcagctc gtctttgtcg gggcattaac cttagggtta
     2101 attatgtttc ccgactatcg ctcacttcta tatgaaaaaa gtgaccgcta gcaatgatgt
     2161 tgccttaggt catttcggca cattgggcta cgtgcttgct ggttcaatcg ggcaactggt
     2221 aggaaaaggc tctaaatcta ccgaagaaat ggatttacct aaaaacctcg gcttcttacg
     2281 tgatagctcc atttctattt cactcacgat gatggcgatt tactatattc tcgcccttgc
     2341 ttcgggcagt gaatacgttt ctacattaag cggcggacaa cattatttag tgtatgccac
     2401 cattcaagcc atcacttttg cagccggtgt ttatgtcatt ttgcaagggg ttcgtttaat
     2461 tttagccgag atcgtcccgg catttaccgg cttctccgaa aaattagtac ctgatgccaa
     2521 accggctctc gactgcccga ttgttttccc ttacgccccc aatgcggtat tagttggatt
     2581 tcttgccagt ttcttaggtg ggattttaag cttagccatt ctagggcaac ttaattgggt
     2641 gttaatttta ccgggcgtag tgccacactt cttctgtggg gcaactgccg gcgtatttgg
     2701 gaacgccacc ggcgggcgtc gtggtgcaat tatcggtgct tttattcacg gcatattaat
     2761 taccttcttg cctgtattct tattacctgt actgggttca ttaggctttg ctaacaccac
     2821 tttctctgac tcagacttcg gcggtattgg gattgtgtta ggctatatgg ctcaatactt
     2881 cgacaagaat atcattacgc tcattattgt cggcttattc gctctgttgg tagcctataa
     2941 ttatttggcg aaaaaaccgg caaaactgaa tacctaatta aaatcaggtg ttatttgctg
     3001 cctgcgagga atcttccctg ccatttcttg atggtaggga attttgttta gttaaacgac
     3061 aacgctcttg ttcaataccc attatccatc acataccccc cactcacttg tttaatcaca
     3121 ccggctaatt ccagacccag taattcaacc agcaatattt caatcgtcaa ctccgtaact
     3181 ttggctaaat catcaattgc catcggctct aagctgatat gctgataaat ttgctgctgg
     3241 caagcggtca gacttggcag attttttgcg aatttttcgc tgtttttgac cgcttgtaaa
     3301 gatgtgctta ctgttggctt agactcatca aacaaaggga tttgctgagg ttgatattgc
     3361 gaggcgacca cctctaaaat atcttccaca ccttccacga gcaatgcccc ttctttaatt
     3421 agcttgtggc aaccttgtgc gtaagggttt tgtaccgcat tcggtaacgc aaacacttcc
     3481 ctaccttgtt caagggcata acgagcggta atcagtgaac cactattaag cgttgcttca
     3541 ataactaaag tgccgagcga taagccacta ataattcgat tacgacgagg aaaattttct
     3601 gccaccggcg gctgattagg cgagaactcc gacaccaatg caccacctaa atcaacaatt
     3661 tgttcggcta attttttatg gcgagcagga tagacttgat ttaatccact ccctaacacg
     3721 gcaatcgtta tgccttgttc cataacgatt ttttgatggg caaaaccgtc aataccaatg
     3781 gctaaaccgc tggtaatgat aatattatgg cgagctaatt cggcagcaaa ttgcccagcc
     3841 caatattcac cataatgaga atagtctcgg ctgccaacaa tcgcaatttg tggcagagaa
     3901 aggctttcca catttccctt tacataaatt aacggtggcg cagtcgaaat ttgttttaag
     3961 agaaacggat aattttcatc aaagagtgtc acaatttgct gatttggcaa ttctgaccaa
     4021 gtcatcgcct gatcaatcca cttttgatca ggattaaacc aacgttgaat ttgcttttca
     4081 ttccaaccta tttgtttgag ttgaagtttg tcatagtggc aaaattcagc aaaattgagt
     4141 tccgccaata atcgccctat acgttgagcc cccatttgcg gcacttgtaa taatcgtaat
     4201 aaatcgtagt gatatgccat attttcatcc tcaaaatttc cctaataata agaaaaggtt
     4261 taaaatagcc aaacgaattt tttaaacttt tcgagagaga tcacaaaaat ggcaatatta
     4321 gatgttgtgc tttaccccga tgagaaactg gcaaccgttt gtgaaccggt ggaacaggtg
     4381 gacgaagcac tgaatcgttt tatcgatgat atgtttgaga caatgtacga acacgaaggt
     4441 attggcttag ctgcaccgca agtgggcgta cttaagcgtg ttattactat tgatattgag
     4501 ggcgacaaaa ccaatcaagt ggtgttaatt aaccctgaaa ttttagaaag cagcggtgaa
     4561 accggcattg aagagggctg tctctccatt cccgggcatc gtgccttagt gccacgcaaa
     4621 gagaaagtga aagtcaaagc ccttaaccgc caaggcgaag aaattatcat cgatgccgat
     4681 gatttatttg cgatttgtat tcaacacgaa atcgaccatt taaacggcgt gctttttgtt
     4741 gaccacatat ccaatttaaa acgccaacgc atcaaagaaa aaatgcaaaa attgaaaaag
     4801 caaattgcac gagcgaaata accaggcaca agcggttaaa atggcttaaa aatttgcaaa
     4861 ttaagtgtga tgacacgata ataaccacag ttcaacttta gaaaggataa aaatatgatt
     4921 atcgtcaccg gcggcgcagg tatgattggc agtaacattg ttaaagcctt aaatgaaatc
     4981 ggtcgcaaag atattttagt agtagataac ctcaaaaacg gggaaaaatt tatcaactta
     5041 gtggacttag atattgcgga ttattgcgat aaagaggatt tcatcgcctc tattatcgcc
     5101 ggcgatgatt ttggtgagat tgatgcggtt ttccatcaag gggcttgctc ggcaaccacc
     5161 gaatgggacg gcaaatactt aatgcaaaat aactacgaat attcaaaaga gttattgcat
     5221 ttttgcttag atcgccaaat tcctttcttc tatgcctcaa gtgcggcaac ctatggcgga
     5281 cgttcggata attttatcga gaaacgggag tttgaaggtc cattaaatgc ttatggctac
     5341 tccaaattcc tgtttgatga atatgtccgc cgtattttgc ctgaggcaga atcaccggtc
     5401 tgtggtttca aatatttcaa cgtatacggg cctcgtgagc aacacaaagg ttcaatggca
     5461 agcgtggcat tccacttaaa caaccagatg ttaaaagggg aaaatccaaa actatttgcc
     5521 ggctcagagc agttcttgcg tgattttgtt tatgtggaag atgtggcaaa agtgaatctt
     5581 tgggcgtggc aaaacagcat ttctggcatt ttcaacctcg gcaccggaaa agcggaatct
     5641 ttccaagaag ttgcgaatgc ggttatcaaa taccacggca aaggcgaaat tgaaaccatc
     5701 ccattcccgg atcatttaaa atcccgctac caaacgttca ctcaagcgga cttaaccaac
     5761 ctaagagccg ctggctacac aggcacattt aaaaccgttg ctgaaggaac agcggaatat
     5821 atggcgtggt tgaatcgcta atcaaacgtt gttaaaaaag cagaaagagc tacgaaatgt
     5881 agctcttttt gttataaggt tataaggatt agaatgctgt cgtatcttgg aataagccta
     5941 cttttaaatc agtagcagta tagatcactc gcccgtccac ttcgacttca ccatcagcaa
     6001 cccccatcac taacttacga ttgaccacac gtttcatatg gatacggtaa gtaacttttt
     6061 tcgctgtcgg taaaatttgt ccggtgaatt ttacttcacc tacgcccaat gcacgccctt
     6121 tgccttcgcc accaatccag ccgagataaa aaccaaccag ttgccacatc gcatctaagc
     6181 ctaagcaacc cggcattaca ggatcgttaa taaaatggca gttaaaaaac cataaatccg
     6241 gtttaatatc aaactcagct tcaatatacc ctttttcaaa attgcctttt tcttggttca
     6301 tttcaatgat gcgatccatc attaacatcg gtggagccgg caattgaggc cctttttcac
     6361 cgaataattc tccttttcct gatagcaaaa gctcttcgta agtatagctt ggtttgataa
     6421 ttggtgtaca actgttcatt atttttaaaa tcctgtaaag tgaaaaaagc ctgttatatc
     6481 aataactgat aaatcaggct gatagtaaca gacttttaat tcagtgaaca gttgtaagct
     6541 gacttgtcgg atcagtctag ctttatcttt ttgcaaattt tgcttaaaaa tgaaccgctt
     6601 gtttcttcac tttcccgctc atctagattc tggtgaatca aggtaaataa tgctgtttgg
     6661 gctgagttag gggctttctc atcatcataa aaatcccgct caaacagaat cgctatggca
     6721 tcaaacacat tttccaccgt ccaaatttta aatagacctt gctccactgc ctcaacaact
     6781 tctgaactta aactcaaatg gctaacacag gccgccggaa taatcacacc ttgtttcccc
     6841 gttaattggc gagcgtgaca aatattaaag aagccttcaa ttttctgatt taccccgccc
     6901 acgcttagca cattcccaaa ttgatcgata gcacctgtca ccgcaataga ttgtggcaag
     6961 ccaactttag ctaaggcact cactagtaca ttgaaaatcg caagagaaga actatcccca
     7021 tcaatttcac cataggattg ttcaaaagca atcgaagctg aaaacggtaa ctgggttggt
     7081 agttctaaca gattcgctaa acaagattga gcgataataa tacctttgga gtggatattt
     7141 ccccctagct ccactttacg ctcaatatct gtaatttcgc catcaccata ttgtacatta
     7201 caactcatgc gtaagggctc gccaaaacta tgcggaatgc cttcaaactc aatgaccgat
     7261 aagccattga tctgcccaat ttcctctcct tcggtttcaa tataaagttg attagtcaga
     7321 atatcaccca ccgtatattg atttaatatc gatgattgct gttctaaata atcaaaataa
     7381 ccttttacct cgtcaatttc ggtcacattt gggtaaaaat tttgaattcc taataaatgt
     7441 tttctcaata atgtagggga aatagacacc aactctcggc tttcactttc acgtatatag
     7501 gcctgtaaaa attgattaag cgctgtggaa gttaatcgct tattgatgag attctcagca
     7561 taattctgaa cataacctcc ccactgctcc acattatccg ataatgataa gtaggatttt
     7621 atttcggtat attgcccaaa ttggtacaac ccatcatcat aagcagataa agtagaaata
     7681 tcttctcttt cccctactaa aatcaattta aatgttgatt tctctttagc cgataaatag
     7741 ggtgagtgat taacagaatt tggctcatat tcaccaaata ataatgcctg ttttagctta
     7801 tcccattggg taatatccaa taagagcgaa cggatattaa caattaaaat tcccttatct
     7861 gcctcgtgaa ttgcaccttt aacatattct gttttcagct ctctttccaa aaacacagaa
     7921 taaccaaata gactattttt attgaaatcc attttgctaa taatcggcag atctacacta
     7981 tattttttca gatattccgt gatttcattg ataaattccg gaaaaagctc agattttaag
     8041 actaataaag caccaaactg agattgtaaa aattgattta acgcttgttt tgcttgaggt
     8101 tgaaaatcta aaaaattcaa tttctcatct aaacgagaga aatcattttg cattactaat
     8161 ttttggctac taagtaaaga aagggtcaca aatctgcctc attattgaaa atttggttta
     8221 ttatacaaaa ttccgctata cttgctaaat atagcttact ttttcctatc atattcagat
     8281 tatgcgatat cagaaactca tcacccaaga aacaacttgg aaatggcaat atctcctaaa
     8341 aaaacatcgt gaaggagaaa atattactaa gtacgaagaa caaagcttga tcgatttaaa
     8401 agttcagttc ctctctactt tgcaaaactc tccggacgaa gtggaaaaat ggattaagtc
     8461 ggaaatgacg cctgagcaac gtaagaaaat gcgtcaatca gtacgcgcca aacgcaaacg
     8521 cttctttaat gctgaaaagc aaacaaccaa gaaaaaatcc attgatttag agtattcgag
     8581 ctggttaagg ctctctaaat atgccaaaag ccaaaaacta acgctttctg aagctattgt
     8641 caaattaacc gatgaactcg aaaataaaca actttactta gagcaagttg ctaaaatgaa
     8701 atcaagttta aaagatttac tgaaataaaa aaagaaaaaa gcccactaac aagcgggctt
     8761 tttttgattc aatttttcaa ctgaataact atattacata gttacttctt ttgaaccttg
     8821 aacttggatt tcaacacgac gatctggtgc taagcaagcg attaatgctt tacgaccttt
     8881 aactgcatca catgtgtgac cagttactgg gtttgcttca ccgtaaccta cagctgttac
     8941 attagctggg ttagtacctt tagaaacgat gtaattagct actgtttcag cacgacgttg
     9001 tgaaagtttt aagttagatg cttctttacc gatgcggtct gtgtaaccat taacttggat
     9061 agctaggttt gctaagccta agttgctgat ttcagcgtgt gctgcatcta aagaagttgc
     9121 tgctgctggt tttaagcttg atttaccgaa atcaaataaa acatcagagc tgaatgcgaa
     9181 gtttttagtt acaacttctg gtgcttcaac tggtgctgca ccttgaccga aacggtatga
     9241 taaacctgca gatactgagt ggatatctgg gctgtattgg aaatttgtac ctcttggtat
     9301 tagataacca actgttgtac gaactgcctt atctaagtta ccaacacgat ttaagtattg
     9361 atattcaaca cgagcagcta attcaggagt aattgcatac tcaagaccag cacctaataa
     9421 tagagatggt tttaagttgt gaactttaat gcgtgaatct ttatctacat gttgttgtac
     9481 gtagtaatcg ttgcgaacta atgcaacacc tactttaccg taaacatcta agttaggaac
     9541 aacttcgtag cttggtttta agcttaagtg cgcaccgtga gctgaatgtt tagccgcagt
     9601 tttttcttta tcatcaccag aaaatgtagt gttaccacga acacgaccaa agtagtcata
     9661 acctaattct gtcgctaaac caaagttgtt ttggtttaag atttggtaac caccgaatac
     9721 accgtaagtt acagagttac ggttaatacc gaggttaata ccgaggttaa taccggatcc
     9781 ataatcatat gtgttgtcaa attgagttaa accgtcgtgg aatgatgccc aaccagcttt
     9841 agcacctgcg tagaaagtgt tagcttgtgg agctgcttga gctactgcag ctgctgataa
     9901 tactgctaat gcaactaatg tttttttcat tttgatgatc ctcttaaata ttgtcatcg
//
LOCUS       DANLUQ010000088         9920 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.088, whole genome shotgun sequence.
ACCESSION   DANLUQ010000088 DANLUQ010000000
VERSION     DANLUQ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9920
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.088"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            249..929
                     /locus_tag="PXB27_001661"
     CDS             249..929
                     /locus_tag="PXB27_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00153 family protein"
                     /protein_id="HDL5346429.1"
                     /translation="MALNNILGLFAQSPLKPLQKHSNKVTECSELLESFFEATFEHDW
                     EKAAEVRGKIVDLERRADALKREIRLKLPRGLFMPVERGDLLELVTQLDKLANYSRDI
                     SGRIIGRKLIIPTEMQPLFKKFLSRSIDASRQVRKVLDEMDQLLETGFRGRELDFVNK
                     MILELDQIEDDTDQYQITLRHTLLELEKNLNPIDVMFLYKCIERISVLADQAQRIGSR
                     IELMLAKS"
     gene            941..2209
                     /locus_tag="PXB27_001662"
     CDS             941..2209
                     /locus_tag="PXB27_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HDL5346430.1"
                     /translation="MELLNQYGSILVIITAIVGFMMAFGIGANDVSNAMGTSVGSGTI
                     TIKQAILIAMVFEFAGAYLAGGEVADTIKSGIIDPAQFANSPDILVLGMMASLCAAGM
                     WLIIATKMGWPVSATHTIIGAVIGFALITIGSDAIQWDALGGIVGSWFVTPILAGIVA
                     YSIFINSQKLIFNRSNPFKQAKKYGPMYMGITIFILVIVTVSKGLKHVGLHLDTAETL
                     GISFALALLAFIIGYFYFRSKSFAMKARAEEKGFAGVEKIFSILMLITACAMAFAHGS
                     NDVANAIGPLAAVESIVRSGGMIEGKTTLAPWILPLGALGMMIGLAIMGKNVMATVGT
                     GITDLTPSRGFAAQFACAVTVVIASGTGLPISTTQTLVGAVLGVGFARGIAALNLGVI
                     RNIVISWVITLPVGAVLSIIIYYILSAIFY"
     gene            2264..2875
                     /locus_tag="PXB27_001663"
     CDS             2264..2875
                     /locus_tag="PXB27_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDL5346431.1"
                     /translation="MKKLSSALLSCFLLGLSSPALTADTYITENLSTFMRKGAGDQFR
                     ISGAIQAGEKVTVLDRKERYSLIRDSRNREGWVLNSDLSDTASPKELIPQLKQQVQDL
                     TNRLSKIDTEWQQRTAEMQRRTQDSVQKSSDLVEENAQLKRELEILKNKNRDLETMHD
                     SEKREIVIQWFIYGGAVLGAGLLLGLLVPFLIPRRRRNNSGWA"
     gene            complement(2929..4227)
                     /gene="aroA"
                     /locus_tag="PXB27_001664"
     CDS             complement(2929..4227)
                     /gene="aroA"
                     /locus_tag="PXB27_001664"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDL5346432.1"
                     /translation="MEKLTLTPISRVEGEINLPGSKSLSNRALLLAALATGTTQVTNL
                     LDSDDIRHMLNALKALGVKYELSDDKTVCVVEGIGGAFKVQNGLSLFLGNAGTAMRPL
                     AAALCLKGEEKSQIILTGEPRMKERPIKHLVDALRQVGAEVQYLENEGYPPLAISNSG
                     LQGGKVQIDGSISSQFLTALLMSAPLAEGDMEIEIIGDLVSKPYIDITLSMMNDFGIT
                     VENRDYKTFLVKGKQGYVAPQGNYLVEGDASSASYFLASGAIKGKVKVTGIGKKSIQG
                     DRLFADVLEKMGAKITWGEDFIQAEQSPLKGVDMDMNHIPDAAMTIATTALFAEGETV
                     IRNIYNWRVKETDRLTAMATELRKVGAEVEEGEDFIRIQPLALENFQHAEIETYNDHR
                     MAMCFSLIALSNTEVTILDPNCTAKTFPTYFRELEKLSVR"
     gene            complement(4273..5367)
                     /locus_tag="PXB27_001665"
     CDS             complement(4273..5367)
                     /locus_tag="PXB27_001665"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDL5346433.1"
                     /translation="MQFINIANEGVKSLSPYQAGKPIEELERELGIQNIIKLASNENP
                     FGFPESAKKAILNQLDDLTRYPDSNGFELKTTIAQKFGVQANQITLGNGSNDLLELFA
                     HTFAGEQDEIIYSQYAFIVYPLVTKAINAVAREILAKNWGHNLDAFLAAITEKTKLIF
                     IANPNNPTGNFLTQAEIEGFLAKVPANVIVVLDEAYTEFTAPQERVDSFGLLAKYPNL
                     IVSRSLSKAYGLAGLRIGYAVSNPEIADLLNRVRQPFNCNSLALASAIAVMNDDEFVK
                     KVAENNRLEMARYEAFCQANGLEYIPSKGNFITIDFKRPAAPIYEALLREGVIVRSIA
                     GYGMPNHLRISIGLPQENERFFTALLKVLA"
     gene            5639..6958
                     /locus_tag="PXB27_001666"
     CDS             5639..6958
                     /locus_tag="PXB27_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDL5346434.1"
                     /translation="MNHLLYSVEDKPPFGLSLLLASQHLLAALGGIIAVPLVLGNVLK
                     LSTADTIILVNAALLISGIVTIIQCRGIGPIGIRLPSVMGTSFTFVAAALAIGFSESG
                     IAGIMGASLVGSLVMIIGSFFMPYIRKLFPPVVTGTVIMMIGLSLIPVAVDWFAGGQR
                     TDPHYATPENLIMASFVLIIVVALVQWGKGIFSAAAIVIGMMIGYIVSLALGWVSFNG
                     VKEAQLFALPQPLHFGLEFPISGIIGMSIAYLVTIVESSGNFLALGNATKTEITGKHL
                     RGGVLCDGLGSAFAAIMSTTPFSSFSQNIGVISLTGVASRYVVAFTGALLVLAGLFPV
                     FGALIVSIPLPVLGGAGLMMFAMIIAAGIQMLDNIQRSKRNGLIIAISIGCGLAVTTR
                     PELLDKLPHFFKEVFGSGITVGSLLALILNLVLPEDKITKKENKSDE"
     gene            complement(7128..9071)
                     /gene="ftsH"
                     /locus_tag="PXB27_001667"
     CDS             complement(7128..9071)
                     /gene="ftsH"
                     /locus_tag="PXB27_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDL5346435.1"
                     /translation="MVKNILLWVVIAIVMMTAYEGFNSNFSGSSNTVPYSTFLSDIKE
                     NRLKGVDFKQNDDTILVTKNDGATYQTVMPMYDEYLMADLAKTNAVITGQPAERRGLL
                     SQILISWFPMLLLIGFYVFYMRQMQGGGGRGAMGFGKNKAKMLTADEVKTRFSDVAGC
                     DEAKEEVGEVVEFLRDPTKFQKLGGRIPKGILMVGPPGTGKTLLAKAIAGEARVPFFT
                     MAGSDFVEMFVGVGASRVRDLFDQAKKNAPCIIFIDEIDAVGRKRGGAGFSGGHDERE
                     QTLNQMLVEMDGFEGSEGVIIIAATNRADVLDDALTRPGRFDRQVTVDLPNVKGREQI
                     LKVHLKKVPLAEGVDPMQIARGTPGYSGAQLANLVNEAALFAARKNKRVVMMEDFEEA
                     RDKINMGPERRSNTMTEKEIINTAYHEAGHVIVGYLMPEHDPLNKVTIVPRGQALGFA
                     QFLPEGDRVSETFTKLESQLSTLFAGRIAEGLIFGEDKITTGASSDIHRATQIARAMV
                     TQWGFSKELGPIFYQNEDGMGAIKGVSEESQKLIDVEMRKIIDRNYQRAKQTLEENMD
                     ILHAMKDALLKYETLDSKQIDDLMARRPVGEPSGWTDTPTKDDNATPPSGEQAADVSS
                     TLEDNPNKDNNIGDSLADTKAEH"
     gene            complement(9192..9818)
                     /gene="rlmE"
                     /locus_tag="PXB27_001668"
     CDS             complement(9192..9818)
                     /gene="rlmE"
                     /locus_tag="PXB27_001668"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HDL5346436.1"
                     /translation="MGRKRSASSSRWLAEHFKDQFVQKAHKQKLRSRAYFKLDEIQQT
                     DRLFKPGMTVVDLGAAPGGWSQYVVTQIGSKGRVIACDILDMNPIVGVDFLQGDFREE
                     SVLNALLERVGEDKVNVVMSDMAPNFSGMPSVDIPRAMYLVELALDMCRQVLATKGSF
                     VVKVFQGEGFDEYLREIRSLFSVVKVRKPEASRDRSREVYIVATGYKG"
BASE COUNT         2805 a         2116 c         1910 g         3089 t
ORIGIN      
        1 tagcaaactt gcataaaata ctctttttaa aagcataata ctctttcgta acatcaccca
       61 aataaggaat aagcgatata aaaaaacaag cataaatttc ttccattttt tgcaaaaaaa
      121 ttattaaaac aaaccgcttg tcttaaaaac ataatttaat ttcgtgatct ttactaatac
      181 atcaagtaaa atacgccgga ctttacagta agaagtcatt gtcaatatta tcactatgga
      241 gatattttat ggcattgaat aacattttag gtttattcgc ccagtcccca cttaaaccgt
      301 tacaaaaaca ctcaaataaa gtgactgaat gtagcgagtt attagaatct ttttttgaag
      361 cgacttttga acatgattgg gaaaaagcag ctgaagtgcg cggtaaaatt gttgatttag
      421 aacgccgcgc agatgccctt aaacgtgaaa tccgcttaaa actgcctcgt ggtttattta
      481 tgccagttga acgcggtgat ttattagaac tagtcacaca attagataag ttagcaaact
      541 attcaagaga tatttccggt cgcattattg gtcgtaaatt aatcattcct accgaaatgc
      601 aaccactttt caaaaaattc ctttctcgta gtattgatgc tagccgtcag gtacgcaaag
      661 ttttggatga aatggatcag ttattagaaa ctggttttag agggcgagag cttgatttcg
      721 tcaataaaat gattcttgaa cttgaccaaa ttgaagatga taccgaccaa tatcaaatta
      781 cattacgtca tacacttctt gagttagaaa aaaacctgaa ccctattgat gtgatgtttt
      841 tatacaaatg tattgaaaga atcagcgttc tagctgacca agcccagcga attggttctc
      901 gtattgaact tatgctggcg aagtcatagg agaaaattaa atggaattac tcaatcaata
      961 tggctcaata ctggtgatta ttactgctat tgtagggttt atgatggctt tcggtattgg
     1021 tgctaatgat gtttcaaatg ccatgggaac atccgtcggc tctggaacaa ttacgattaa
     1081 acaagctatt ttaattgcta tggtatttga atttgctggt gcttatctgg caggcggtga
     1141 agtcgcagat acaattaaaa gtggcattat tgatccggca caattcgcca atagccctga
     1201 tatcctggta cttggaatga tggcatctct ctgtgctgcc ggaatgtggc taattattgc
     1261 aactaaaatg ggttggccgg tttcagcaac tcacacaatc attggtgccg ttattggttt
     1321 tgcattaatc actattggtt cagatgcaat tcaatgggat gcattaggtg gaattgttgg
     1381 gagttggttt gtgaccccta ttcttgcggg gatcgtagca tattcgatct ttattaactc
     1441 acaaaaactg atctttaacc gctcaaaccc attcaaacaa gcgaaaaagt atggcccgat
     1501 gtacatggga attactattt ttattttagt tattgtaacc gtgtcaaaag gcttaaagca
     1561 cgtaggatta catctagata cagctgaaac attgggaatt tcattcgcgc ttgccttact
     1621 tgcttttatc attggatatt tttatttccg cagtaaaagc tttgcaatga aagctcgtgc
     1681 agaagagaaa ggcttcgctg gcgttgagaa aatttttagt attttaatgc ttattacggc
     1741 ctgtgctatg gcttttgctc atggctctaa tgatgttgct aatgctattg ggcctctagc
     1801 tgctgttgaa tctattgtaa gaagtggtgg aatgattgaa ggtaaaacga ctttagcccc
     1861 ttggattctc ccactaggcg ccttaggtat gatgattggt ttagctatta tgggcaagaa
     1921 tgtaatggct acagtaggta ctggtattac cgaccttact ccaagccgtg gctttgctgc
     1981 tcaatttgcc tgcgccgtca cggttgtcat tgcatcaggt actggtttac caatatcaac
     2041 aacccaaacg cttgtaggag cagtattagg tgtgggattc gcccgtggta ttgctgcact
     2101 taacttaggc gttattcgta atattgttat atcatgggta attaccttgc ctgtaggtgc
     2161 tgtgctttca attattatct attatatttt aagcgctatt ttctattaat ttttcaaaaa
     2221 gaatacaggc aactgtattc ttcctagctt tagaggaata tatatgaaga aattatcatc
     2281 agctcttctg tcttgttttt tactcggcct atctagccct gctttaacgg cagacactta
     2341 cattactgaa aatctgagta catttatgcg caaaggtgct ggtgatcaat tccgcatttc
     2401 aggcgctatt caagcaggtg aaaaagtcac agtattagat cgtaaagagc gctacagtct
     2461 gattcgagat agtcgtaacc gtgaaggttg ggtacttaat tcagacttaa gcgatactgc
     2521 tagtccaaaa gagctgattc ctcaattaaa acaacaggtt caagatttaa ccaatcgcct
     2581 gagcaaaatc gataccgaat ggcaacaacg taccgcagaa atgcaacgta gaacgcaaga
     2641 ttcagtgcag aaaagcagtg atttggttga ggaaaatgca caactaaaac gtgaactgga
     2701 aatcctgaaa aacaaaaatc gtgatttaga aacaatgcac gattccgaga aacgtgaaat
     2761 cgtgattcag tggttcatct atggtggtgc tgtgttaggc gcaggtttac tacttgggtt
     2821 actcgtgcct ttcctgatcc ctcgccgcag acgtaacaac agcggttggg cttagtctgc
     2881 cacaataaaa cgtaaaaagg attcagtttt ctgaatcctt ttttactttt atctgaccga
     2941 taatttttcc aactccctaa agtaagtcgg gaacgtttta gcggtacaat ttggatctaa
     3001 gatcgtcact tctgtattcg ataacgcaat taatgaaaaa cacattgcca tacggtgatc
     3061 gttataggtt tcaatttcag cgtgctggaa gttttctaac gcaagcggtt gaatccgaat
     3121 aaaatcttcc ccttcttcta cctctgcccc gactttacgc aattcggttg ccattgctgt
     3181 caagcggtcg gtttctttta cccgccagtt ataaatattg cggataactg tttctccttc
     3241 ggcaaataaa gcggttgttg caatcgtcat tgccgcatca ggaatatgat tcatatccat
     3301 atctacgcct tttagcgggg attgctcggc ttgaataaaa tcctctcccc aagtgatttt
     3361 tgcccccatt ttttccaaca catcggcaaa caagcggtcg ccttggatcg attttttacc
     3421 aatgcccgtt acttttacct tgcctttaat cgcaccggag gctaagaaat aagaggcaga
     3481 agaggcatct ccctccacca aataattacc ttgtggagca acatagcctt gtttaccttt
     3541 aactaaaaag gttttgtaat ctcgattttc aaccgtaata ccaaaatcgt tcatcatcga
     3601 aagggtaata tcaatataag gttttgatac cagatcaccg ataatctcaa tttccatatc
     3661 gccttccgct aatggggcag acatcagcaa tgcggttaga aattggctgg aaatcgagcc
     3721 gtcaatttgc acttttccgc cctgcaaacc gctattgcta attgccaacg gtggatagcc
     3781 ttcattttct aaatactgta cctctgcccc tacttggcgt aaagcatcga ctaagtgttt
     3841 aatcgggcgt tctttcattc ttggttcacc ggtaagaatg atttgggatt tttcctcacc
     3901 ttttaaacac aatgctgctg caagtggtcg cattgccgtg cctgcattgc cgagaaacag
     3961 tgataagccg ttttgaacct taaaagctcc accaatccct tcaactacac agacggtttt
     4021 atcgtccgat agctcatatt tcacgcctaa cgcttttaag gcattgagca tatgtcgaat
     4081 atcatcacta tctaataaat tggtcacttg agtcgtaccg gtggctaagg cggctaataa
     4141 taaggctcgg ttagacaggc ttttagaacc aggtaaattg atctcgcctt ctactcggga
     4201 aatcggggtt aaagttagtt tttccatttt tctctttaaa tttgtaaaat tttcataaaa
     4261 ggtaaccgct tgttaagcta acactttcaa taaggcagta aaaaatctct cgttttcttg
     4321 cggtaaaccg atactaatgc gtaaatgatt cggcattcca taacctgcta tcgagcgaac
     4381 aatcacgcct tcacgcagta atgcctcata aatcggagcg gcaggacgtt tgaaatcaat
     4441 agtaataaaa ttgcccttag aagggatata ttccaagccg tttgcttggc agaaagcctc
     4501 atagcgagcc atttccaagc ggttattttc tgccactttt ttgacaaatt catcatcatt
     4561 cattaccgca atcgctgaag ctaaggcaag actattgcag ttaaacggct ggcgaacacg
     4621 atttaataaa tccgcaattt cagggtttga aaccgcataa ccaatccgaa gcccggctaa
     4681 gccgtaagcc ttagagagtg aacgtgacac aattaaattc gggtattttg ccaataagcc
     4741 aaacgaatcc acccgttctt gaggcgcagt aaactcggta taagcttcgt ccagcaccac
     4801 gattacattt gccggcactt tagctaaaaa gccctcaatt tccgcttggg ttaaaaaatt
     4861 accggtcgga ttattcgggt tggcaataaa aattaattta gttttttcgg taattgcagc
     4921 caaaaaggca tctaaattgt gtccccaatt tttagccaga atttcacggg caacggcatt
     4981 aatcgctttc gtcaccaacg gatacacaat aaacgcatac tgagaataga taatttcatc
     5041 ttgctcaccg gcgaaggtgt gggcaaacag ctctaataaa tcgtttgagc cattgcctaa
     5101 ggtgatttga ttggcttgta cgccaaattt ttgggcaata gtggttttta attcaaagcc
     5161 gtttgaatcc ggatagcggg ttaaatcgtc aagctgattc agaatcgcct tttttgcact
     5221 ttcaggaaaa ccaaatgggt tttcgttcga ggctaattta atgatatttt gaatccctaa
     5281 ttcacgctct agctcctcaa tcggctttcc tgcttggtaa ggcgaaaggg atttcacgcc
     5341 ctcatttgcg atgttgataa attgcataat aacctctgat tattcttata aaatataagt
     5401 tcggattgta gtaaagaatg attgccttta ccagtaaatt ttttgttaca actggaaaga
     5461 aattggtaca aacattcgga ttagctttgc aaaaagcgta aaaaatctta ccgcttgtca
     5521 tttatcagtt agggaaaata aaaaatcact ctcttatata aaaacaatta cgcaaacgat
     5581 tgcttattaa aaaaacttca gtataatgcc gacatttttt aactctcgag gaatcactat
     5641 gaaccattta ctttattctg tcgaagacaa accgcctttc gggctgagct tattgcttgc
     5701 ctcacaacat ttattagccg ctttaggtgg cattatcgct gtacctttag tgctagggaa
     5761 tgtactcaaa ctctctactg cggatactat aatcttagtt aatgctgcac ttttaatttc
     5821 aggcatcgtc actattattc aatgtcgagg tattggccca atcgggatta gactaccgag
     5881 tgtaatggga actagcttta cgtttgttgc agcagcatta gcgattggtt tcagtgaaag
     5941 tggcattgcc ggcattatgg gagcctcttt agttggctct ctagtgatga ttatcggcag
     6001 tttttttatg ccttatatcc gtaagctttt tccacccgtg gtaacaggta ctgtcataat
     6061 gatgattggc ctaagtttaa ttcctgttgc agtagactgg tttgctggtg gtcagcgtac
     6121 tgatccacac tatgcaaccc cagaaaatct tataatggct agttttgtat taattattgt
     6181 cgttgcttta gtacaatggg ggaaaggtat tttttcagct gctgctattg tgattggtat
     6241 gatgataggt tatatcgttt cgctagctct gggttgggta agttttaatg gcgtgaaaga
     6301 agcacaactt tttgcgttac cacaacctct acattttggc ttagaatttc caatctccgg
     6361 tatcatcgga atgtcaattg cttatttagt taccattgta gaatctagcg gaaatttctt
     6421 agcactaggc aatgcaacta aaactgaaat taccggaaaa cacttacgtg gaggtgtatt
     6481 atgtgatggt ttaggctcag catttgcggc aattatgtcc acaactccct tttcttcttt
     6541 ctcacaaaat attggggtca tttcattaac cggtgtagcc agtcgctatg ttgtagcatt
     6601 tacaggcgca ttacttgtac tagccggttt atttcctgta tttggagcat taatcgtgtc
     6661 tatcccgtta cctgtgcttg gcggtgcagg cttaatgatg tttgccatga ttatcgcagc
     6721 cggtatccaa atgttagata atattcaacg tagcaaacga aacggtttaa ttattgctat
     6781 ctctatcggt tgtgggctgg ccgtaacaac tcgacctgag ctattggata agcttccaca
     6841 cttctttaaa gaagtttttg gctcaggtat tacggtcggc tccttattag cacttatttt
     6901 gaatttagtt ttacctgaag ataaaataac caagaaagaa aataagagtg atgaataaat
     6961 caccctaaaa aaacaaggac agctcacaat acaactttga tgctgtcctt tattcccata
     7021 gagagcatat ttaaatttag gttaataaaa aaatctgcac tctatattgg tgtttacctt
     7081 tagagtacag atttcttctt attctaactt tctagcaaac aattagatta atgttccgct
     7141 ttggtatcgg ctaacgaatc cccaatgtta ttatctttat ttggattatc ctctaaagtt
     7201 gaacttacat ccgctgcctg ttcaccactt ggtggagtgg cgttatcgtc tttagttggg
     7261 gtatctgtcc aacctgatgg ttcacccacc ggacggcgtg ccattaaatc atcaatttgc
     7321 ttgctgtcta aggtttcgta tttcaataag gcatctttca ttgcgtgtaa gatgtccata
     7381 ttttcttcca aagtttgctt cgctcgttga tagttgcggt caatgatctt acgcatttcc
     7441 acatcaatca atttttgcga ctcttccgat acgcccttaa ttgcacccat tccatcttca
     7501 ttttgataga aaatcgggcc taattctttt gagaagcccc attgagtaac cattgctcgt
     7561 gcaatttggg ttgctcgatg aatatcagag gaagcccctg tggtaatttt atcttcacca
     7621 aaaatcaagc cctcagcaat tcgccctgca aatagggtgg aaagttggct ttccaatttg
     7681 gtaaatgttt cgcttacacg gtcaccttcc ggtaagaatt gagcaaaacc taacgcctga
     7741 ccacgaggca caattgtcac tttattcagc ggatcgtgtt ccggcattaa atagccaaca
     7801 ataacgtgtc cagcttcgtg gtaagcagta ttgatgatct ctttttccgt cattgtattt
     7861 gaacggcgtt ccggccccat attgattttg tcacgggctt cttcgaaatc ttccatcatt
     7921 accacacgtt tattcttacg ggcagcaaat aaggcagctt cgttcaccaa gtttgctaat
     7981 tgagcaccag agtaacccgg cgtaccacga gcaatttgca tcgggtcaac tccctctgca
     8041 agcggtactt ttttcaagtg aactttcaaa atttgctcac gccctttcac gttcggcaaa
     8101 tcgaccgtga cttggcggtc gaaacgcccc ggacgagtta aggcatcatc taacacatcg
     8161 gcacggttgg ttgcagcgat gataatcaca ccttcagagc cttcaaagcc gtccatctca
     8221 actaacattt ggttgagggt ttgttcacgc tcatcgtgtc caccgctgaa acctgcacca
     8281 ccacgtttac gccctaccgc atcaatttca tcaataaaaa taatacaagg ggcatttttc
     8341 ttggcttggt cgaataaatc acgcacccgt gaagcaccca cacctacaaa catttccacg
     8401 aagtcggaac ctgccattgt aaagaatggc actcgtgctt cacctgcaat cgctttcgcc
     8461 aataaggttt tacccgtacc cggagggcct accatcaaga tcccttttgg aatgcgccca
     8521 cctaattttt gaaatttagt tgggtcacgc aaaaactcaa caacttcgcc tacttcttct
     8581 ttggcttcat cacagccggc tacatcagaa aatctggtct taacttcgtc tgcggtcaac
     8641 attttggctt tatttttccc aaagcccatt gcaccacgtc ctccgccacc ttgcatttgt
     8701 cgcatataaa acacatagaa accgattaac agcaacattg ggaaccaaga aatcaaaatt
     8761 tgcgagagca agccacgacg ctcagcaggc tgaccggtaa tcactgcatt ggtttttgct
     8821 aaatctgcca ttaagtactc atcatacatc ggcattacgg tttggtaggt tgcaccatca
     8881 tttttcgtca ccaaaatggt gtcatcattc tgtttgaaat cgaccccttt taaacgattt
     8941 tctttaatat cgcttaaaaa agtcgaatat ggcactgtat ttgagctacc actgaaatta
     9001 gaattaaaac cttcataggc tgtcatcatc acaatggcaa tcaccaccca aagtaaaata
     9061 tttttaacca tatcgttcaa ggttaatccc ctatctgctg aataaaaaac tgttcaagat
     9121 tactataaaa gcggtcgaat tttccaattt ttttgcaaaa tgttaataaa aaatgaccgc
     9181 ttgcttgttt tctagccttt ataacctgtc gctacaatat aaacttctcg agaccgatct
     9241 cgagacgcct ccggttttct gacttttacc acactaaaga gcgagcggat ttctcgaagg
     9301 tattcatcaa atccttcgcc ttggaatacc ttaaccacaa aactgccttt ggttgctaat
     9361 acttggcgac acatatccaa tgccaattcc actaaataca ttgcacgagg aatatcaacc
     9421 gacggcatac cgctgaaatt gggagccata tccgacatca ccacattcac tttgtcttcg
     9481 cctactcgct caagtaacgc attcagcacc gattcttcac ggaagtcgcc ttgtaagaaa
     9541 tccacgccta caatcgggtt catatctaaa atatcacaag cgattactct gcccttactg
     9601 ccgatttgcg tcaccacata ttgcgaccaa ccgcccggtg cggcaccgag atccaccacc
     9661 gtcattcccg gtttgaataa acggtcggtt tgttgaattt catctaactt aaaatacgcc
     9721 cgtgaacgca atttttgttt atgtgctttt tgcacaaatt gatctttaaa atgttctgca
     9781 agccaacgtg atgaactcgc acttcgtttt ctacccattt gttcttctat cttaattaat
     9841 tcaacgattt ataataaggg ttaaattagc aaattcaaga gatgttctaa aatttagctc
     9901 ggcttattat gcttaattaa
//
LOCUS       DANLUQ010000089         9870 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.089, whole genome shotgun sequence.
ACCESSION   DANLUQ010000089 DANLUQ010000000
VERSION     DANLUQ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9870
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.089"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            176..1882
                     /locus_tag="PXB27_001669"
     CDS             176..1882
                     /locus_tag="PXB27_001669"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HDL5346437.1"
                     /translation="MNTSSFNRTWAKVILSALMRYGVKHFCIAPGSRSTPLTLAAVQL
                     QQTQYAECHTHFDERGLGFYALGIAKATDSPVAIIVTSGTAVANLYPAVIEASLSHQK
                     LIILSADRPTELIGCGVNQAIHQQGIFANYPVASVNLPKANEAYHASWLVSTIEQACT
                     TQAQQGGVLHINVPFEEPLYEADINEILNTPWLRPIQGWLNNAQTKWIDQQAIQSEVS
                     MHENWDYWRTKRGVIVVGKLPPEQGTGLKTWAETMGWCLLSDVQSCIEASLPYADIWL
                     SNETVKQRLLQADIVIQFGTQIVSKRVNEFLKAFQGEYWVVEQSHDYHNPNAHHQTRF
                     IAKAHHFLRVHPPLRQKPWLLEPLALSQFCSTFIEQQVGGSLSEASLAHNIQDVLAIN
                     GNLFIGNSLFVRLVDALCKLPEGYPVYTNRGASGIDGLIATMAGIAKGSDRPTVGVVG
                     DISALHDLNSIALLTKINQPTILFVVNNSGGALFDMLPVDSKAKDQYYRLAHNFEFAQ
                     VATMFGLEYIRPFTWADLKTKLKHAYARKGVTIVEIKVNEHDGSNMYKSLLKQISQAE
                     IA"
     gene            1931..2770
                     /gene="menH"
                     /locus_tag="PXB27_001670"
     CDS             1931..2770
                     /gene="menH"
                     /locus_tag="PXB27_001670"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HDL5346438.1"
                     /translation="MLSYQWHAQQGTPVVFLHGLLGSQQDWQAVLQHLQNFPQIRPLT
                     IDLPFHGNSKHITCADFANLRQQLHATLESLIGSRPFYLVGYSLGGRAALDYVLNMEN
                     PNLQGVVLEGANIGLSTENDRQIRRQNDRHWANRFRQEPIEQVLEDWYKQPVFADLSD
                     TERECYIDSRKHNQGEQIAQMLEATSLAKQPDYTEKLKQTDKNIVFFIGEKDQKFRKM
                     ASSHQLPTQLIANAGHNAHRANAKNFVEKLGLLILHSDSDLRSQSVKHNGAALLHKSP
                     SGA"
     gene            complement(2819..3496)
                     /locus_tag="PXB27_001671"
                     /pseudo
     CDS             complement(2819..3496)
                     /locus_tag="PXB27_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251475.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            3582..4244
                     /locus_tag="PXB27_001672"
     CDS             3582..4244
                     /locus_tag="PXB27_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HDL5346439.1"
                     /translation="MRKTHSFQQVRKIKPTWMSVSGHIPFRNGVSIPYESTLERDFLM
                     YFTYLPSVDEVVSQPTTLPFVKNGITYTYTPDFFLSFTDGRKPMLIEVKPKAKWQKHW
                     KEWKEKWKAAIRFCQENGYVFHVYDEDRIRHLALFNLNYVQRYKRIQHEQEDIDAILA
                     QVKLMGNTTIDYLLSRFFAGSLYRMKGLQIIYHLLATKQLHCNWFLPLNEFTEVWGNN
                     DE"
     gene            4234..6162
                     /locus_tag="PXB27_001673"
     CDS             4234..6162
                     /locus_tag="PXB27_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HDL5346440.1"
                     /translation="MMSNSGFTEKRYHHRLDRGRIILQKGNIYLNREDGEQYELVDYM
                     DEPSQLLVRNLNTRTTKVVSIHQLENFKMNERTDLSVDVTAISNEYWEKAQQKYEAIK
                     PLLGMDQHRPYAVKARAEDVGVNPRTLYRWLQAYNSIGSIAGLVDQKRGWQQGNSRLT
                     PEQDKLIVQVINEFYLHKQRPTTEQTIREIRRRCKIEKVESPSKETIRMRILHISEEE
                     RLRKRGQREKARNKFKPKPNSFPDADYPLSVVQIDHTPVDLIIVDSKYRKPIGRPFLT
                     VAIDIYSRMIVGYYLSLDAPSVTSVAMCIARGILPKERLLLDLGLQGSEWNAFGYPVK
                     VHVDNGPDFQALDLSKSCSAHGIHLEFRPMGRPEYGGHIERVIGTFMKEVHSLAGTTF
                     SNIKERDSYDSEKEAIMTLDEFEKWLVHYIVNVYHKRVHSALGISPEQKWKIGIFGDE
                     NEVGCGYPQLPVDEQTLLLDFLPSITRTIQHNGVTIDGLRYYDVALNMYISDSDESGK
                     SKEFLFRRDPRNISKIWFYDPKLKRYFPIPFANQAMPEMSIWEYREVRSRIANKGDKY
                     INEQQVLDGLTEMREMVAESAQRTKKARRQAERQKMHKASKPIIETKVETKAVVPVVV
                     TSNLLALDDESLSFGEVD"
     gene            6162..7049
                     /locus_tag="PXB27_001674"
     CDS             6162..7049
                     /locus_tag="PXB27_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012478339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniB family NTP-binding protein"
                     /protein_id="HDL5346441.1"
                     /translation="MQYEHVHEKFRHLVTADNQERIAFLDEPRWLGYGVAKDIMDNLV
                     SLMNKPKRPRMLNLLIVGDSNNGKTTLIRRFFDLYGQAYIDSDSNAIYPILLAEAPPS
                     ANEKELYISLLERFYVPYKPTDTIAKLRYQTIHLFREFRVKMLVIDEFHSLLVGTPRL
                     QRQVMNAIKMLCNELQIPIVGVGTRDAIRVLHTDRQHASRFDVAELPIWKLDKEFQKL
                     LFQFQGILPLRESSNLQSPELATKIHTISGGNLGNVHRLLTACAVQAITSGTEKITLD
                     IIEKNSWVQPTNGYRKIIG"
     gene            7007..8113
                     /locus_tag="PXB27_001675"
     CDS             7007..8113
                     /locus_tag="PXB27_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HDL5346442.1"
                     /translation="MGTTNKRLSQNNRIKKGVIHTPIYSNESISSWLIRASFDCGTDP
                     LTFTGFYWEKHRLWTLDLDRGLENIEPTIYDDVQALSGKIQLSEYSLFRRLYPINADE
                     TLAKGQVRWVIPRSARNRTHHIGQQYCPLCLDESPHLRNDWRLAWNFGCLKHNMMLAN
                     RCFCCGTLYQPHLLSAEKRHLNHCHQCGGKLEQGTMEMSLAEKLTLQGINQVLEKDSG
                     ICFGYKVSGKVWFDMLRYCINLLRRASDRPNHALAKFVVNLQIDVENICSPKTGLSFE
                     QLPLGERTNLLVNAYHILGMEADSIRSAIYLCGGTQKTFMFEKYPDFWQEFMALAPAG
                     PNRTRKRTKPQNESITAINRRWERLKRKLHLNNE"
     gene            8110..9534
                     /locus_tag="PXB27_001676"
     CDS             8110..9534
                     /locus_tag="PXB27_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346443.1"
                     /translation="MKINVIKPQEEPCSACGKISRIWRRYNGEGYCANCYAKWFPKKE
                     CSCCHQPKRIYLSSTVCWDCEKQTDCVRCGKAAGTFKIGKITRYGAVCNVCSRYFREE
                     RECFECGQLSRTMLRASESGHSELLCLKCFQKYRFATCKNCRRYRKVHDRDNQLCRKC
                     SEQRTSQCPKCKQDMPAGYGNICADCSRRTLLFNLIRMNVHLFRHKKVKRAYKKFIFW
                     YLAKCGVSVVLKKSDDHVLFFMRCDENWGNIPDYRELVTFFKPNGLRTHLTVLRWLLD
                     TNQVKVDESLKEEMAGLERIQSLFNKLSNSVPCIANYYTQLQHKYEAGKTSLKSVRLA
                     LQPAIDFISSESVSLLPTQVQLNSYLVAKTGQIAAITGFINHLNREYHCELEIDRSLI
                     QKMKEKQLKQQAKQRLAILYKQEKLSIKEQNELLSVVLLNLHNIWIKSPKKLSISEID
                     GIAYYVDNEKKYFIPKDIHIRLME"
     gene            9649..9855
                     /locus_tag="PXB27_001677"
     CDS             9649..9855
                     /locus_tag="PXB27_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA delta(2)-isopentenylpyrophosphate
                     transferase"
                     /protein_id="HDL5346444.1"
                     /translation="MTLKAQFREWLKKEQEHDEMIGFAHIPCILSKVDSKLAIVSGNA
                     YEKKYLRYWIKCFRFDRNISKYIK"
BASE COUNT         3054 a         1989 c         2127 g         2700 t
ORIGIN      
        1 taaatgctct taaaaaagag agagtttact aagtaaagtg ttgctcaata gatagaaatt
       61 ttcacgaagt tttacaaaaa cattccaatt ttctcctaaa aaacttccca caataatttc
      121 actttttgct acaatcctaa ccatttatcg aatttatcaa tcagtagaga agaatatgaa
      181 taccagttct tttaatcgca cttgggcaaa agtgatttta agtgcattaa tgcgttatgg
      241 cgtaaaacat ttttgtattg ctccgggttc tcgctcaaca ccgctcacat tagcggcagt
      301 gcaacttcaa caaacgcaat atgcagagtg ccacactcat tttgatgagc gtggtttagg
      361 tttttatgcg ttagggattg ctaaagcaac cgacagcccg gttgctatta ttgtcacttc
      421 cggcacagca gtagcgaacc tttatcctgc ggtgatagag gcaagcctga gccaccaaaa
      481 actgattatt ctttctgccg accgcccaac cgaattaatc ggctgtgggg taaaccaagc
      541 cattcatcag caaggtattt ttgccaatta cccggttgcc agtgtcaatc taccgaaagc
      601 gaacgaggct taccacgcaa gctggctggt ttcgaccatt gagcaagcct gcactaccca
      661 agcacaacaa ggcggtgtgt tgcatatcaa tgtgcctttt gaagagccgc tttacgaagc
      721 agacattaat gaaattctaa atacaccttg gttacgccca atccaaggct ggttgaataa
      781 cgctcaaacc aaatggatag accaacaagc gattcaaagc gaagtctcaa tgcacgaaaa
      841 ttgggattac tggcgaacta aacgaggggt gattgtggtc ggcaaactgc cgccggaaca
      901 aggcacaggt ttaaaaactt gggcggaaac catgggttgg tgcttgctaa gtgatgtgca
      961 atcttgcatt gaagccagcc tgccgtatgc cgatatttgg ctctccaacg agactgtcaa
     1021 acagcgttta ttgcaagcgg atatcgtgat tcaattcggc acgcaaattg tcagcaaacg
     1081 ggtgaatgaa tttttaaaag cattccaagg ggaatattgg gtggtggaac aatcgcacga
     1141 ttaccataat ccaaacgctc atcatcaaac ccgttttatt gccaaagcac atcacttttt
     1201 gcgggtacac ccaccacttc gccaaaagcc atggttgctt gagccattgg cattatcgca
     1261 attctgctcc actttcatag agcaacaagt gggtggcagc ttaagtgaag cctcacttgc
     1321 acacaacatt caagatgttt tagcaattaa tggcaactta tttatcggca atagcttatt
     1381 tgttcgattg gttgatgctc tgtgcaaact gccggaaggc taccctgttt acaccaaccg
     1441 tggtgcaagc ggtattgatg gcttaatcgc aacaatggca ggcattgcaa aaggtagcga
     1501 ccgcccgact gtcggtgtgg taggtgatat ttccgcatta cacgatctta actcaattgc
     1561 attgctcacc aaaattaacc agccgaccat tttatttgtg gtgaataaca gcggtggtgc
     1621 gttgtttgat atgttgcctg ttgatagcaa agcgaaagat caatattacc gccttgctca
     1681 caactttgaa tttgctcaag tcgccacaat gttcgggctt gaatatatcc gcccgtttac
     1741 ttgggcagat ctaaaaacca aactcaaaca cgcctatgct cgtaaaggtg ttaccattgt
     1801 ggaaatcaaa gtaaatgaac acgacggcag caatatgtat aaatcattgc taaaacaaat
     1861 ttcacaggcg gaaattgcct aatcacaact gcccctcgcc aatcctgcga ggggtaaaaa
     1921 tgtatcgact atgctttcct atcaatggca cgctcaacaa ggcacacccg tggttttcct
     1981 tcacggcttg ctcggctcac aacaagactg gcaagctgtt ctccaacatt tgcaaaattt
     2041 tccgcaaatt cgaccgctta ccattgacct tccttttcac ggaaatagta agcacattac
     2101 ctgtgccgat tttgccaacc tgcgacaaca actacacgcc acccttgaaa gcctaatcgg
     2161 ctcacgacct ttctatttag tcggctactc actcggtgga cgagcggcac tcgattatgt
     2221 gttaaatatg gagaatccga atctgcaggg cgtggtgtta gaaggggcga atatcggctt
     2281 aagcacagaa aacgaccgcc agattcgccg gcaaaatgac cgccattggg caaaccgttt
     2341 ccgtcaagaa cccatcgaac aagtgcttga agattggtac aaacagcctg tttttgcgga
     2401 tttaagcgat acggaaagag aatgttatat tgatagcaga aagcacaatc aaggagagca
     2461 aattgctcaa atgctggaag ccaccagcct cgccaaacag cctgactaca ccgaaaaact
     2521 caagcagacg gataagaata ttgtcttttt tatcggtgaa aaggatcaga aatttcgcaa
     2581 aatggcatca agccaccaac taccaacaca actgattgct aatgcagggc ataacgctca
     2641 tcgggctaat gctaaaaatt ttgtggagaa attggggtta ttgatactcc actccgacag
     2701 cgacctacgg tcgcaatcgg ttaagcataa cggtgcagct ctgctgcaca aaagccccag
     2761 tggggcttaa tcagccgtag gctaaatata acctagtacg gctcagccgt gctaggtgtt
     2821 agggctcccc ctgcaaccgt tgcaataagc gatccatcgc ccgataacca agtgcttccg
     2881 ctaaatgcag ttgttgaata ttcgcctcgc cggctaaatc agcaatagtc cgagaaactt
     2941 tcaaaatacg gtggtaggct cgtaccgata aaccgagctt tgtcaatgca gtttctaaaa
     3001 atagggcatc ttgtgccgat aattggcaat ctcgctcaat ttctcgggtg gtgagttgag
     3061 aattgatttt acctgctcgc tcaaattgca tttttctggc taaaaacact cgcttgcgaa
     3121 cctgctcggt ggtctctcct cgattctccg tcccactttg caatgctcct tgtggcaaaa
     3181 gtggcacttc tatagagaga tcgaaacggt ctaagaaagg tccggaaagg cggtttaaat
     3241 accgcattac ctgttggggc gaagtgcggt tatgcgtgcc ttgataatgc cctgtcgggc
     3301 ttgggttcat cgctgcaatt aattgaaagc gtgccggaaa ttgtacttta gcgttggctc
     3361 gggaaatcac aatttcgcct gcctccaacg gctgacgtaa cgcatcaagc actttacgtt
     3421 caaattcagg caattcatct aagaacagca caccgttatg ggcaagtgaa atttcccccg
     3481 gtttggggat cgagccactg taaaaactag aaataaatgt cataaaaact agaaatagct
     3541 gtcagattat ttagaaaaca agaaataggt gtcaaattat tatgagaaaa acacatagtt
     3601 ttcaacaggt tagaaaaatt aaacccactt ggatgagtgt atcggggcat attccattta
     3661 gaaacggagt cagtattccc tatgaatcga ctttagagcg tgattttttg atgtatttca
     3721 cttatctacc ttcagtagat gaagtcgtct ctcaaccgac taccctacct ttcgtcaaga
     3781 atgggatcac ttatacctat acaccagatt tcttcttgtc atttacagac ggtcgaaagc
     3841 cgatgttaat cgaggtcaaa ccgaaagcaa aatggcagaa gcactggaaa gaatggaaag
     3901 aaaaatggaa agctgcaata cgtttttgcc aagaaaacgg ttatgtattc catgtgtatg
     3961 atgaagatcg gattcgacat ctagcgcttt tcaatctcaa ctatgttcag cgctataagc
     4021 gaatacaaca tgaacaagaa gatatagatg ctattctagc ccaagtaaag ctgatgggaa
     4081 ataccactat tgactattta ctctctcgat tttttgcagg ttctctatat cgcatgaaag
     4141 gcctacaaat catctatcac ttactggcaa cgaaacaact acattgcaat tggtttctac
     4201 cacttaatga atttaccgag gtatggggga ataatgatga gtaactctgg atttacagaa
     4261 aagcgctatc atcaccgatt agatcgggga cgtattattt tgcaaaaagg taatatttac
     4321 ctaaatagag aagatggaga acagtatgaa ttggttgatt atatggatga accctcccaa
     4381 ctgctcgtac gaaatctcaa cactcgtaca accaaagtcg tcagtattca tcagcttgaa
     4441 aatttcaaaa tgaacgagcg aacggatctt tctgtggatg taaccgctat tagtaatgaa
     4501 tattgggaga aagcccaaca aaaatatgag gcaattaaac cgttattagg gatggatcaa
     4561 catcggcctt acgcagttaa ggctcgtgca gaagatgttg gggtgaaccc tcgtacatta
     4621 taccgttggt tacaagccta taattccata ggatctatcg caggtttagt cgatcaaaaa
     4681 cgtggatggc aacaaggaaa ttcccgttta acgccagagc aagacaagct gatcgtgcag
     4741 gtaatcaatg agttttacct acataaacaa cgcccaacta ctgagcaaac tattagagaa
     4801 attcgccgcc gatgcaaaat tgaaaaagtg gaaagcccaa gcaaagaaac catccgtatg
     4861 cgcattttgc atatttcaga ggaagagcga ttacggaaac gcgggcaacg agaaaaggca
     4921 cgcaacaaat ttaagccgaa gccaaatagc tttcctgatg cggattatcc attgagtgtg
     4981 gtacaaattg accatacacc agttgaccta attattgtgg atagtaagta tcgtaaaccg
     5041 attggccgac cgtttttgac ggttgcgata gatatataca gcagaatgat tgttggttat
     5101 tacctttcac ttgatgcgcc ttctgtaact tcggtggcga tgtgtattgc acgtggtatt
     5161 ttgccgaaag aacgcctact gcttgattta ggtttgcaag gctcagaatg gaatgctttt
     5221 ggctacccag tgaaagtgca tgtggataat ggcccagatt ttcaggcttt ggatttatct
     5281 aaatcttgtt ctgcacacgg aatccatctt gaatttagac cgatggggcg acctgaatat
     5341 ggcggacata ttgagcgtgt tatcggtaca tttatgaaag aagttcacag tttagcagga
     5401 acaacattct ccaacatcaa agagcgtgac tcttacgatt cggaaaaaga ggccattatg
     5461 actctcgatg agtttgaaaa atggttggta cattacattg tgaatgttta ccacaaacgt
     5521 gtgcattctg ctttaggcat ttctcctgaa caaaaatgga agattggtat ttttggagat
     5581 gaaaatgaag tgggatgtgg ttatccgcaa cttcctgttg atgaacaaac actactactt
     5641 gatttcttgc caagtatcac tcgtaccatt cagcataatg gtgtaacaat tgacggactt
     5701 cgttattacg atgttgcgct aaatatgtat atcagtgatt cagatgaaag cggtaaatct
     5761 aaggaatttc tgtttcgccg agacccgcga aatatcagca aaatttggtt ttatgatccg
     5821 aaattaaaac gttacttccc aattccattt gcaaatcagg caatgcccga aatgagcatt
     5881 tgggaatata gagaggttcg tagccgaatt gcgaataaag gtgataaata tatcaacgag
     5941 caacaagttt tagacggttt aaccgagatg cgtgaaatgg ttgctgaatc ggctcaacgg
     6001 acgaaaaaag cacgccgtca ggctgaacga caaaaaatgc acaaagcaag taagccaatt
     6061 attgaaacaa aagtggaaac taaggctgtt gtaccagtcg tagtaacatc gaatttgctt
     6121 gctttagatg atgagagctt atcttttggt gaggtggact aatgcaatac gaacatgttc
     6181 atgaaaaatt ccgccatctt gtgacagcgg acaatcagga gaggattgcg ttcttggacg
     6241 agccaagatg gttaggttat ggtgtcgcca aagacattat ggataatttg gtttctttaa
     6301 tgaataaacc aaaacgccca cgaatgttga atttgttgat tgtcggcgat tccaataatg
     6361 gaaaaaccac actgatccgc cgcttcttcg acttgtacgg tcaggcgtat atcgacagtg
     6421 attctaatgc gatttaccct attcttcttg ccgaagcccc accaagtgcg aatgaaaaag
     6481 aattgtacat ctcattatta gagcggttct acgtaccata taagccgaca gatacgatag
     6541 ccaaacttcg ttatcagact atccatttgt tccgagaatt tcgtgtcaaa atgttggtta
     6601 ttgacgagtt tcattctctg ttggttggta caccacgctt acaacgacag gtgatgaacg
     6661 caattaagat gctttgtaac gagctacaaa ttccaattgt cggtgttggt actcgtgatg
     6721 caattcgggt attacatacg gatcgacaac atgccagtcg ttttgatgtt gcggaattgc
     6781 caatatggaa actagataag gaatttcaaa aactcctatt tcaattccaa gggattttac
     6841 ctttgaggga gagttcaaat ctgcaatctc cagaacttgc tacgaagatc cacaccattt
     6901 caggtgggaa cttagggaac gtgcatcgct tactaacagc ttgtgcagtt caagccatta
     6961 caagtggcac ggaaaaaatc accttagaca tcattgagaa aaattcatgg gtacaaccaa
     7021 caaacggcta tcgcaaaata atcggataaa aaaaggtgtt attcataccc ctatttattc
     7081 aaatgaaagt attagctcgt ggttaattcg tgcatccttt gattgtggta cagatccact
     7141 gacttttaca gggttttatt gggaaaaaca tcggttgtgg acattagatt tagatcgtgg
     7201 cttggaaaat attgagccaa ctatctatga tgatgtacag gcattatcgg gtaaaatcca
     7261 actttcagaa tattccttgt ttcgcaggct ttaccctata aatgcagatg aaaccttagc
     7321 taaaggtcaa gtaagatggg tgattccacg ttcggcaagg aatagaaccc atcacattgg
     7381 tcaacaatat tgtcctttgt gcttggatga gtcgccacat ttacgcaatg attggcggtt
     7441 agcgtggaat tttggttgtc tcaagcataa tatgatgttg gcgaacagat gtttttgttg
     7501 tggtacattg tatcaacccc atttactatc agcggaaaag aggcatttga atcattgtca
     7561 tcaatgtggt gggaaactgg aacaaggcac aatggaaatg agtctagctg aaaagctgac
     7621 attgcaaggt attaatcagg tactcgaaaa agattcaggc atctgttttg gatacaaggt
     7681 gtcgggtaag gtgtggtttg atatgctccg ctactgcatc aacttattga gacgtgcatc
     7741 agacagacca aatcatgcct tagcaaaatt tgtagttaat ctacagattg atgttgaaaa
     7801 tatatgtagc cccaaaacag gactttcttt tgaacaactt ccgctaggtg aacgtacaaa
     7861 tttacttgtg aatgcctatc atattttggg aatggaagca gattctattc gtagtgcaat
     7921 ttatttgtgt ggcggaacac aaaaaacctt tatgtttgaa aaatatcctg atttttggca
     7981 agaatttatg gcactagcac ccgcaggccc aaatagaaca cgaaaacgca ccaagcccca
     8041 aaatgagtct atcacagcaa ttaatcgccg ttgggagcgt ttgaaacgca aactacactt
     8101 aaataacgaa tgaaaattaa tgtaataaaa ccacaggaag aaccttgctc cgcttgtggc
     8161 aaaatttctc gcatttggcg cagatataat ggcgaaggct actgtgccaa ttgctatgct
     8221 aagtggtttc cgaaaaaaga gtgcagctgt tgccatcaac caaagcgtat ctatttgagt
     8281 tcaacagtat gttgggattg cgaaaaacag actgactgtg tacgttgcgg taaggctgcg
     8341 ggtacattca aaattggtaa aattacccgt tatggtgcag tttgtaatgt gtgttcacgt
     8401 tattttcgtg aagaacgaga atgttttgag tgtggtcaac tatctagaac tatgcttcga
     8461 gcatcagagt caggtcatag cgagctactc tgcttgaagt gttttcaaaa atatcggttt
     8521 gcgacttgta aaaactgtcg ccgttatcgc aaagttcatg accgtgataa tcagctttgt
     8581 cgaaaatgta gtgagcaacg aacttcacaa tgcccgaagt gcaaacagga tatgcctgcg
     8641 ggatatggta atatttgtgc agattgttct aggcgaactc tactgttcaa ccttattcga
     8701 atgaatgtgc atttattccg ccacaaaaaa gtgaaaagag catataaaaa gttcattttt
     8761 tggtatctcg caaagtgtgg cgtgagtgtt gtgctgaaaa aatcagacga ccacgtcctc
     8821 ttttttatgc gttgcgatga aaattggggc aacattccag actaccgtga gttggttaca
     8881 ttctttaaac ccaatggatt acggacgcat ttgactgtgt tgcgttggtt gcttgatacc
     8941 aatcaagtca aagtagatga gagcttaaaa gaggaaatgg cggggttgga acgtattcaa
     9001 tccctattta acaaattaag taactctgta ccttgtattg caaactacta cacgcagtta
     9061 caacataaat atgaggcggg caaaacatca ctgaaatctg ttcgattagc tttacaacct
     9121 gcgattgatt tcatcagttc agaatctgtc tcgttactgc cgactcaagt acaactgaat
     9181 agctatcttg tggcaaaaac gggacagatt gctgctatta caggctttat aaaccacctg
     9241 aaccgagaat atcattgtga attagaaatt gaccgctccc tcattcaaaa gatgaaagaa
     9301 aagcagttga agcagcaagc aaaacagcga ttagcgatac tctacaagca agaaaaacta
     9361 agtatcaaag agcaaaatga gctactctca gtcgtattac tgaatttgca caacatttgg
     9421 ataaaatcac cgaaaaaatt aagtatttca gagatagacg gtatagccta ctatgttgat
     9481 aatgagaaaa aatactttat accaaaagac attcacatac gcctgatgga ataagctgtc
     9541 gtcaattttt atttttgcga tctgtgtcga gaaaataata tttatgaatc gacagatgaa
     9601 ataatattga taaagtctct ttgtgtgttc aacaaacagg agataaatat gacattaaag
     9661 gctcaatttc gtgaatggct taaaaaagag caagaacatg atgaaatgat agggttcgcc
     9721 catatccctt gcattttatc taaagtggat agcaaactgg cgattgttag cggaaatgct
     9781 tatgagaaga aatatctcag atattggatc aagtgtttta ggttcgatag aaatatcagc
     9841 aagtatatta aataggttaa atatctagct
//
LOCUS       DANLUQ010000090         9861 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.090, whole genome shotgun sequence.
ACCESSION   DANLUQ010000090 DANLUQ010000000
VERSION     DANLUQ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9861
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.090"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            139..798
                     /locus_tag="PXB27_001678"
     CDS             139..798
                     /locus_tag="PXB27_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL5346445.1"
                     /translation="MFNISKQQVLDAFHFRSACRSYDPNKKINKEDMDYILELGRLSP
                     SSVGSEPWKFLVLQNPETRQKIAPVSWGIKHPMEEMSHLVVILTKKNVRYDSVYMRDM
                     LAKRGLTYEQIEASLVRYKSFQEEDMKLLSERELFDWSTKQTYIALGNMMTGAALIGI
                     DSCPIEGFHYDTVNQILSDEGLFDLNEYGVSCMITFGYRNKEIKKKSRKPTEEVIAWI
                     E"
     gene            complement(858..2183)
                     /locus_tag="PXB27_001679"
     CDS             complement(858..2183)
                     /locus_tag="PXB27_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HDL5346446.1"
                     /translation="MLIFNLIVFAAILFLLAKIYKSTQKLGQTVFIGLFLGLASGAFL
                     QTFYEKATVDATLDWINLIGNGYVRLLQMIVMPLVFVSILSAITRLKEAGSLGKISFS
                     VLSVLLVTTAIAAAIGIALVYLFDLSAEGLVAGSRELAAQEKVVGRVEQVSNLSVPSM
                     LLSFIPKNPFLELTGANPTSIINTVIFSALLGIAALSLGKEDKALGERIAQGVETLNK
                     LVMRLVRFVIRLTPYGVFALMIKMAATSQWSDIVNLGSFIVASYIAIGLMFIVHGILL
                     FVAKINPLDYYKKVLPTLSFAFTSRSSAATIPLNIETQTNKLGNNSVIANFSATFGAT
                     IGQNGCAGIYPAMLAVMVAPTVGMDPFSLNYILTLILVVAISSFGIAGVGGGATFAAI
                     VVLSTLNLPIELVGLLISVEPIIDMARTALNVNGAMVAGTLTNKWVKAE"
     gene            complement(2284..3036)
                     /locus_tag="PXB27_001680"
     CDS             complement(2284..3036)
                     /locus_tag="PXB27_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon-containing protein"
                     /protein_id="HDL5346447.1"
                     /translation="MYQAIVHFSYHNFEQDPVTAISQILNQWLYNGQVIGREMPITFH
                     QNEFQARVCVPTQESLLPQYNSDEVNEALQQAVEIGVIFASFELVGRDYQGEESSHNR
                     SPAFQILYTTHLDTCSPLYDGEQFAPIPLYSLQDQSLSEAILEWQQNWQACDLLQMNG
                     NLLECHALVQISDETSDLAMIGRDLCQQIEQKTHIPTFYYLYRLGRDESEYQRKCPCC
                     GGEWKLTEPLHDIFHFRCEPCRLISNLSWELL"
     gene            complement(3036..3941)
                     /locus_tag="PXB27_001681"
     CDS             complement(3036..3941)
                     /locus_tag="PXB27_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HDL5346448.1"
                     /translation="MNEKYSKLVKNAANLAIIVAILLIVIKAFAWWKTGSISILAAMT
                     DSVVDLFASLTNILVLRFALQPADENHAFGHGKAESLAALAQSAFISGSAVFLLLQGF
                     HKLTNPELIAQSSIGVIVSIVSIVITALLVFYQRYVVTLTKSPAIEADSLHYQTDLLM
                     NAAILVAMLLNSAGFVYADAVFAIGIALYIAFNAVKMFWCAVQTLLDQALPDEEIERI
                     KEIASQHPNILGIHDILTRRAGAVRFIQLHLELDDHLTLLVAHEITDSLEQKLLAAFP
                     MSHVIIHQEPTTVVKQEFQDMAENI"
     gene            complement(3950..4513)
                     /locus_tag="PXB27_001682"
     CDS             complement(3950..4513)
                     /locus_tag="PXB27_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATPase"
                     /protein_id="HDL5346449.1"
                     /translation="MSAIKKGITLVALSLAGCVSQSPQFAVPDAIHFGQDTFVKVTHS
                     QIDEMQHLLYLPEKSEKNPENWQKGILFFLDKNSKNQTLEQRAAFRQASFAKKPELEA
                     KVEIQQNELHSSVIYPPIERFNNVMLEVTRGRNLACGYGQIQFADKREVGKTVAKKSP
                     NLTAYSQEIARLSLELNQLGWQVQCSK"
     gene            complement(4513..5289)
                     /gene="yaaA"
                     /locus_tag="PXB27_001683"
     CDS             complement(4513..5289)
                     /gene="yaaA"
                     /locus_tag="PXB27_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HDL5346450.1"
                     /translation="MLAIISPAKTLDFKTNIDGFAFSQPNLTACSQELIDICKQLSPA
                     ELGSLMSISDKLAALNVARFAEWQLEHNEQNAKAALFAFKGDVYTGLEAETLSKAQVE
                     YAQQHLRMLSGLYGLLKPLDLMQPYRLEMGTKLANPKGKDLYAFWGNIITENLQQAID
                     EQGDDILVNLASDEYYGAVKPQNLNATIIKPVFLDEKNGKYKVISFYAKKARGMMVRF
                     ILDTQPTSIEQLKQFNYGGYWFDADSSSESELVFKREEQA"
     gene            complement(5478..7198)
                     /gene="ltrA"
                     /locus_tag="PXB27_001684"
                     /pseudo
     CDS             complement(5478..7198)
                     /gene="ltrA"
                     /locus_tag="PXB27_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012341372.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(8880..9275)
                     /gene="rpsI"
                     /locus_tag="PXB27_001685"
     CDS             complement(8880..9275)
                     /gene="rpsI"
                     /locus_tag="PXB27_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDL5346451.1"
                     /translation="MTAANQNYGTGRRKSSSARVFIKPGSGNITINQRSLEVYFGRET
                     ARMIVRQPLELVELTDKLDLYITVKGGGISGQAGAIRHGITRALMEYDETLRPALRAA
                     GFVTRDARRVERKKVGLHKARRRPQYSKR"
     gene            complement(9292..9720)
                     /gene="rplM"
                     /locus_tag="PXB27_001686"
     CDS             complement(9292..9720)
                     /gene="rplM"
                     /locus_tag="PXB27_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDL5346452.1"
                     /translation="MKTFVAKPETVQRDWYVVDATGKTLGRLATELARRLRGKHKPEY
                     TPHVDTGDYIIVINADKVAVTGKKQTDKIYYWHTGYVGGIKDATFKEMIARRPEAVIE
                     IAVKGMLPKGPLGREMFRKLKVYAGSEHNHQAQQPQVLDI"
BASE COUNT         2821 a         2199 c         1819 g         3022 t
ORIGIN      
        1 tgatttttaa ggtaaagcaa ttttaaacaa gcggtcattt tttcctaatt ttttgcaaat
       61 taacaggcgt gaaattcgca ttctgctcta aatcaagtta tcattaatct acttatttca
      121 cttataaaag gaaaaacgat gtttaatatt agcaaacagc aagttttaga cgcttttcac
      181 ttccgttcgg cctgtcgcag ttacgatcca aacaaaaaaa tcaacaaaga agatatggat
      241 tatattttgg aactcgggcg tctctcacca agctctgtcg gttctgaacc gtggaaattt
      301 ttagtgctac aaaacccgga aacccgtcag aaaatcgccc cggtttcgtg gggaattaaa
      361 cacccaatgg aagaaatgag ccatttagtg gtgattttaa ccaaaaagaa tgtccgttat
      421 gattctgtct atatgcgtga tatgttagcc aaacgaggct taacctatga gcagattgaa
      481 gcctcgctgg tacgctataa gtcgttccaa gaagaggata tgaaattgct tagtgaacgt
      541 gaattattcg attggagtac caagcaaacc tatattgcat taggcaatat gatgaccggt
      601 gcggctctga ttggtattga ctcttgcccg attgaaggtt ttcattacga taccgtaaac
      661 caaattttat cggacgaagg cttatttgac ctaaacgaat atggtgtgtc ttgtatgata
      721 acttttggct atcgcaacaa agaaattaag aaaaaatccc gcaaacctac cgaagaagtg
      781 attgcgtgga ttgaataatc accttaactt ataaaaacgg gcgaattaat cgcccatttt
      841 tatatcttta gttttgacta ctcggctttc acccatttat tggttaatgt gcccgccacc
      901 attgcaccat tcacgtttaa ggcggtgcga gccatatcaa taatcggctc aactgaaatg
      961 agcaagccta ctaactcaat cggcagattc aaggttgata gcaccacaat cgccgcaaaa
     1021 gttgcaccac cacctacacc ggcaatgcca aatgaggaaa ttgccaccac caaaatcaag
     1081 gttaaaatat agtttaagct aaacggatcc attcccactg ttggagcgac catcaccgcc
     1141 aacattgccg gataaattcc cgcacagcca ttttgcccga tggttgcccc aaaggttgca
     1201 gaaaagttgg caatcacact gttattacct aatttattgg tttgcgtctc aatattcaac
     1261 ggaatggttg ccgcactgga acgagacgta aaagcaaaac ttaatgtcgg caacactttt
     1321 ttgtagtaat ccaacgggtt gattttagcc acaaagagta aaataccgtg cacaataaac
     1381 atcaaaccaa tcgcaatgta tgatgccaca ataaagctac ctaaatttac aatatcgctc
     1441 cattgagaag ttgccgccat tttgatcatc aacgcaaata cgccataagg ggttaggcga
     1501 atcacaaaac gtactaaacg cataaccagt ttatttaagg tctctacacc ttgtgcgata
     1561 cgttcgccca acgctttatc ttctttgcct aagctcagtg cagcaatgcc tagcagagcc
     1621 gagaaaataa ccgtgttgat gatcgacgtt gggtttgcac cggttaattc taaaaacgga
     1681 tttttcggaa tgaaagaaag caacattgaa ggcacagata agttgctcac ctgctccaca
     1741 cgccctacca ctttctcttg agcggcaagc tcacgggagc cggcgactaa accctcggca
     1801 gataaatcaa acagatacac taaagcaata ccaatcgctg cggcaatcgc ggtggtcact
     1861 aataacactg atagcacact aaaactaatt ttgcctaaag atcctgcctc ttttaagcgg
     1921 gtaattgccg ataaaataga gacaaacacc aatggcatta cgatcatttg tagcaagcga
     1981 acataaccgt taccaattaa gttaatccaa tcaagagttg catcaacagt agctttttcg
     2041 taaaaagttt gcaaaaaagc accgcttgca agccctaaaa ataacccaat aaacactgtt
     2101 tgaccgagtt tttgcgtaga tttataaatt ttagccaata aaaataatat tgccgcaaag
     2161 acaattaagt tgaaaataag cacagaacct ccaaagtaaa accgcttatt ataaaaatgc
     2221 cgcaagggta ttacgcttgc ggcattcatt caagatgatt tattactttg gtataactaa
     2281 aagttataat aattcccagc ttagattaga gatcaaacgg caaggctcac atctaaaatg
     2341 gaaaatatcg tgcaacggct cagttaattt ccattctccg ccacaacaag ggcatttgcg
     2401 ttgatattca ctttcatcac gccctaaacg gtagaggtaa taaaaggtgg gaatgtgagt
     2461 tttctgctca atttgctgac atagatctcg accgatcatt gcaagatcgc ttgtttcatc
     2521 actaatttga acaagtgcgt ggcactccag cagattgccg ttcatttgta gcagatcgca
     2581 cgcttgccaa ttttgctgcc attccaatat cgcttcgcta agagactgat cctgtaaaga
     2641 ataaagcgga attggggcaa actgctctcc atcataaagt ggcgagcagg tatctaaatg
     2701 ggtggtatat aatatttgaa acgctggcga gcggttatga ctgctttctt cgccctgata
     2761 atctcgcccg acaagctcaa aacttgcaaa aattacgccg atttcgaccg cttgttgcaa
     2821 cgcttcattt acttcatcac tattatattg tggcaaaagg ctttcttgtg taggcacaca
     2881 aacccgagct tgaaactcgt tttgatgaaa agtaatcggc atttctctgc caatgacttg
     2941 cccgttatat aaccattgat ttaagatttg gctaattgcc gtcaccggat cttgctcaaa
     3001 attatgatag ctgaaatgca ctatcgcctg atacattaaa tattctccgc catatcttga
     3061 aattcttgtt tgaccaccgt agtcggctct tgatgaataa tcacgtgaga catcggaaat
     3121 gccgccagca atttctgctc aaggctatca gttatttcgt gagctactag cagcgttaag
     3181 tgatcgtcta attccaaatg taattggata aaacgcactg cacctgcacg gcgggttaga
     3241 atatcgtgaa tccctaaaat gttcggatgt tgcgaagcaa tctctttaat ccgctcaatt
     3301 tcttcatcgg gcaaagcttg gtctagcaag gtttgcaccg cacaccaaaa catttttact
     3361 gcattgaaag caatgtagag ggcaatacct attgcaaaaa ctgcatcggc gtacacaaaa
     3421 ccggcactat taagcaacat tgccactaaa attgccgcat tcattaataa atcagtttgg
     3481 taatgtaacg aatccgcttc aatcgccgga cttttcgtaa gcgtgacaac ataacgctga
     3541 taaaagacta aaagtgcagt aatcacaatt gatacaatac tgacaattac cccaatactc
     3601 gattgagcaa ttaattccgg attggtgagt ttatggaaac cttgtagcag taggaaaacc
     3661 gccgagccgg aaataaacgc actttgggca agtgccgcca aagactccgc tttgccgtgc
     3721 ccaaacgcgt gattctcatc tgccggttgt agtgcaaaac gcagcactaa aatattggtt
     3781 agcgaagcaa acagatccac taccgaatcc gtcattgctg ccaagattga aatcgaaccg
     3841 gttttccacc atgcaaaggc tttaatcaca atcagtaaga tcgcaacaat aatcgctaaa
     3901 ttcgctgcat ttttgactaa tttgctgtat ttttcgttca tttactacat tatttactgc
     3961 attggacttg ccaacccagt tggtttaatt caagagaaag tctagcaatt tcttggctgt
     4021 aagcggtcaa attcggcgat ttttttgcaa ctgtcttgcc aacttcccgc ttatctgcaa
     4081 attgaatctg cccgtaacca caagctaaat tccgcccacg ggttacttct aacatcacat
     4141 tgttaaaacg ttctataggt ggataaatca cactgctgtg taattcattt tgctgaattt
     4201 ccactttcgc ctcaagctcc ggctttttcg caaaacttgc ttggcggaat gcagctcgtt
     4261 gctctaaggt ttgattttta ctgtttttat ctaagaaaaa taaaatgcct ttttgccaat
     4321 tttccggatt tttctcactc ttttctggca aataaagtaa atgttgcatt tcatcaattt
     4381 ggctgtgggt gacttttaca aaagtatcct gcccaaagtg aatagcatca ggcactgcaa
     4441 attgtgggct ttggctgaca caacctgcca atgataacgc aaccaaagtt attccctttt
     4501 tgatagccga cattatgcct gctcctcacg tttaaagact agctcgcttt cactagaact
     4561 gtctgcatca aaccaatagc ccccgtagtt aaactgtttg agctgttcga tgctagtcgg
     4621 ttgggtgtct aatataaagc gaaccatcat tccacgggct tttttggcat agaagctgat
     4681 cactttatat ttaccgtttt tctcatctaa aaacaccggc ttgataatag tcgcatttag
     4741 attttgtggc ttaaccgcac cataatattc atcagaggca agattaacta aaatatcatc
     4801 gccttgttca tcaatcgctt gctgcaaatt ttcagtaata atattgcccc agaaagcata
     4861 aagatctttg cctttcggat tggctaactt agtgcccatt tctaaacgat aaggctgcat
     4921 taaatctaac ggctttaata aaccatacaa gcctgaaagc atacgcaaat gttgttgagc
     4981 atattcaact tgtgctttgc ttaacgtttc tgcctctagt ccggtataaa catcaccttt
     5041 aaaggcaaag agggcggctt tggcattttg ttcgttatgc tcaagctgcc actcggcaaa
     5101 gcgagccaca tttaaagcgg ctaatttatc gctaattgac atcaaactac ctaattcagc
     5161 aggtgaaagc tgtttacaaa tatcaattaa ttcttggcta caagcggtca aatttggttg
     5221 agaaaatgca aaaccgtcaa tattggtttt aaaatcaagg gttttggcag gggaaataat
     5281 ggctaacata aatgcctcta tcaaatagtt aatcagggcg gttattctac cacagtcata
     5341 aagacaaaaa aggcaggaaa ccctgccttc ttgaaaacga aaagaaaagt cgggtagcga
     5401 aagtacatta ctgcacctcc gccccctaag aactgtacaa gcaaatttct ctgcatacag
     5461 ctcaagcctt tcatcagcta tatagccggc ttcacgacct ccaagttcaa agtatgaatc
     5521 tttatgtgac aagtcggatg taccaaacaa cgattgctta atgcatcgct tcctcctaac
     5581 attttgtaca cgatatgatg atcgtgccag cctgtttctt tggttattgg caacccacat
     5641 aatgcacatt ttccacgctg gtctttatac agtgcctgcc attgtcgtct gtgtgattgt
     5701 tcttcataca gacgttcttg gctcagtttt tcgccgtaca actcccattc aggcaaaaac
     5761 ggattatatt cagacttaat tttaacgtgt cgtttgattt taacatagcc acaatagaat
     5821 aagtctatcg gcttagtttc tcctttttca ttggtataaa ctgtgccaaa tacccaattt
     5881 cgagtcgctg tggtacaaaa gtatttctct tttatccaca taataccttt attgtgatga
     5941 cggcgttcac accatttcca aagtactttc caaactaatg cgtccattcg tccgaatgcc
     6001 ttacttgcaa cttggcttcg atgataattt gcccaacctc gtaacattgg gtttagcacc
     6061 attataagat cttcctgttt agccattttc atttagaaat tacctctttt accttgttgt
     6121 agaacgcttt cacattcttt tgacttggtc tgttcagtat ttttcctttg aaacgtctca
     6181 cattccaacc tagaaaatca aaaccttctt caatatgtac cactttcgtc ttttctgttg
     6241 ataaggataa acctcgttca ttcagaaagt tctggataag tgggataact tgattttcaa
     6301 gtaactcttt cgttttacct gatatgataa agtcatctgc ataacgtact aagtaagttt
     6361 tattttctcg aattgtccga ctgccttttc ctccaaaatg agtttctaac agtttttcca
     6421 atccatctaa tgccatattt gcaagcgttg gcgagataat cccaccctgt ggtgtacctt
     6481 cctctgttcg tttcaaccgt ccaaattcca ctacacctga ttttagccat ttccgcaata
     6541 tccgtttatt cattggaata tgctttaata gccagtcgtg gcttatgtgg tcaaaacaac
     6601 ctgcaatatc cgcatcaagt acccactcaa caggttgatt agttttcttg cctttatgcg
     6661 aataaattta gtgtatatga gtaatagcat ctaccgttga acggtttaat ctaaatccat
     6721 aagaaccttt atccgcagtt gtttctgcca caggttgtat tgccaataaa taaagggctt
     6781 gcattgctct atctcgcatt gttggaatac ccaacggacg tttctttcca ttcggtttgg
     6841 ggatatacac tcgtcttaat ggactaggtt tatacccttt ttccgtcaat gatagtgcgg
     6901 cttgccattt tgtctctggg gtctcccaaa gctctttatc gacaccactt gttcgcttac
     6961 cttgattttc agtcactctt cttactgcaa ctaatttagc ataatgtgag tgggttagca
     7021 ttcgttgtaa gtttttaacc ttacgccaat tgccttccag tgttgccttc gcaatccgaa
     7081 tttgcattcc tttcacatac gtttcagctt tatgccaatc gacataatgc catttggaga
     7141 tgtgcgaggt cgctggattg caagctaact tttcagtata atgcttacgc tctttcatac
     7201 ctacctctga tattacaaga taagtcgaaa gtctcagcaa gtgggtagat ccttcaagta
     7261 aacaaatccc tattgagata aactatcctt agtttattcc ctttggttac aagaggatct
     7321 taccgtttta ccgataattt caaccgctct tgtaaccgat tgggttaaag agtcgagcgc
     7381 taggttatga tgagcctaac gctcatttct ttaatttgtt tttgtaccta ttcaacattg
     7441 tattccttga taacattcaa tttctcttct gttatctaca atctctttcg gtgttaccct
     7501 taaaggaaac gctattcatc ttacgatgaa agaccagtta gaagtctgca ccctttcggg
     7561 ttaggctatg tttcacaccc tatctatgcc attacagcat agcattcgct ttttctaacc
     7621 tcccttacct gcattcccaa cagtatcgct tacgctttac ttgccttttc ctcttttacc
     7681 ttcgccatct atttcaaacg gtttgagtat tgtgggcaag gcagaaatac aggcttacca
     7741 agttccatta ctatcacacg agtagtgaag atacagtctc tcccccgaca gtgctttgat
     7801 ggcgtatcct cacgttaaag aagaataacc gactgcctac cttttggtca gagcctatca
     7861 atagctttgg ctctctctcg ataacgaggt ttatcaactg ttcacttacg tttatctttc
     7921 tacccagtct agccccttga aggtgtgcta ttcactttct cccaatgtcc tctcacgatt
     7981 tcattgactg attttcatca ggggttcatt gtcaggaagc tccgcaccac accgttaccg
     8041 gtgtcgcact atccctagac tactgttagc catataacag gttaccttat cattccctcc
     8101 gtactactca tatcttcgga ttgtttgacc gtaacaatga cagtaatagc ttacgcatac
     8161 ttactaagtc agtatcctat actgcataaa taagtccgaa cctttacaaa ataaattcac
     8221 ttatgcgact aaagtcgctt tgtctatatc caaattttcc cgcaaatagc ctataacctg
     8281 tccaataggc gaaggtagct aaccctagct aatgtgagta ttagaggtga ttttaacgag
     8341 aatttaaggt ataattatcc ctagctcata aaaacgaata aacaagagtt tatccttagt
     8401 gtttttattc actattgagg agcattctac gattaccgta gattgcgagt gtgaaaagta
     8461 taagtaaaga tggcactcct tccctaagat ttaccaagtc ttagttctga tgtcttttct
     8521 gagtgctgag gcgatctatt ggactttatt tatagtaggt gttgtaaatt ttctctgctc
     8581 tggggtatgg gtaactccgg tctgcggatc ttgctttctt actctatctt ggtattaaga
     8641 aaaaaaccaa gtacatcaaa gtgaagatca taccttacag cctttcgagt tggttcattt
     8701 actttcccaa cttagaacaa gagtgcttgg gcaaagcccc ttttctcttg cctgtctttc
     8761 caagcactcc gaagagtgct tggaaagtcg gaagcaggtg ataagaatac cacccacttc
     8821 cagcaataac tccttgatat ataaggagtt attgccataa gtacgacaac ttgtcgcact
     8881 taacgttttg agtattgtgg acgacgacgt gctttgtgta aacccacttt tttacgttca
     8941 acgcgacgtg cgtcacgagt aacgaagcct gctgcacgaa gtgctgggcg tagcgtttca
     9001 tcgtattcca ttaatgcacg agtgataccg tgacggattg cacccgcttg accagaaata
     9061 ccaccacctt taacagtgat gtaaaggtct aatttatcag ttaattcaac caattctaac
     9121 ggctgacgta cgatcatacg agctgtttca cgaccgaagt aaacttcaag tgaacgttgg
     9181 ttgatagtaa tgttacctga acccggtttg ataaatacac gggctgaaga gcttttgcgg
     9241 cgacctgtgc cgtagttttg atttgctgct gtcattttta tgctccgtga attagatatc
     9301 taaaacttgt ggttgttgtg cttggtgatt gtgttctgaa cctgcgtaaa cttttaattt
     9361 acggaacatt tcacgaccaa gtggaccttt tggtaacata cctttcactg cgatttcaat
     9421 cacagcttca ggacgacgag caatcatttc tttgaaggtt gcatctttga tgccacctac
     9481 atagccagtg tgccagtagt agattttgtc agtttgtttt ttacctgtta ctgctacttt
     9541 gtctgcattg atcacgatga tgtaatcacc ggtatcaacg tgtggagtgt actcaggttt
     9601 atgtttacca cgaagacggc gtgccaattc agtcgctaaa cgacctaatg ttttacccgt
     9661 cgcatctact acataccagt cacgttgtac tgtttctggt tttgctacaa aagttttcat
     9721 taattaaata ccaaatttaa agtttaaaac ccaatacctc attgaggtat ttcctattat
     9781 tttacggtga acccgacccc ttcgagtctg attcagacca aacttgtgtg gcggggaaaa
     9841 ccacacaaaa acagggtggc g
//
LOCUS       DANLUQ010000091         9798 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.091, whole genome shotgun sequence.
ACCESSION   DANLUQ010000091 DANLUQ010000000
VERSION     DANLUQ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9798
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.091"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            179..1657
                     /gene="fruB"
                     /locus_tag="PXB27_001687"
     CDS             179..1657
                     /gene="fruB"
                     /locus_tag="PXB27_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HDL5346453.1"
                     /translation="MLNLSAKNIRLNGSASNKEEVIRFVAAELVAKGNVAAGYEVGML
                     NRETQTSTFLGNGIAIPHGTLDTRDLVKETGVQIIQLPQGVEWGEGNTAYVVIGIAAK
                     SDEHLTLLRQLTTVLSDEEAAATLAKTQNLDEFIAVLSGKKNLPALSQELISLNIESS
                     SPITLSAINAAKLQEQGYVSQAFISDVVANKALHLGNNLFVVDSAKGNQANGIAIARN
                     TQGQTLLTVAETDNALQPLLTQLLKPEVRSQFAHFSAEQMLGLLKGESVASTPVTNVE
                     ASGNQVVGTFTIRNDNGLHARPAAVLVQTVKPFSSKISVENLDRNTAPASAKSAMKVV
                     ALGASQGHRLRFVAEGEDAQQAIEALQQAIINGLGESVSFVPAVADTIESIAVSAIPA
                     EETATAVDANTKEATFVIKNEHGLHARPSAVLVNEVKKFNAKIEVQNVDKNSPLVSAK
                     SLMKIVALGVTKGTTLRFVASGDDAEVALAAIGAAIEAGLGE"
     gene            1671..2615
                     /gene="fruK"
                     /locus_tag="PXB27_001688"
     CDS             1671..2615
                     /gene="fruK"
                     /locus_tag="PXB27_001688"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDL5346454.1"
                     /translation="MRIATVTLNPAFDLVGKLQRIEIGEVNTVETLGLYPAGKGINVA
                     KVLADLGTKLSVTGFLGEENQGDFVNAFKHNDLADEFYRINGKTRINVKITETEADVT
                     DLNFLGFEISESDWEKFVTLSHQWESKFDLVAVCGSLPRGVTPEKFASWLESLHRQGL
                     KVVLDTSNAALTAGLTAHPWLVKPNRRELEVLVGHSLNSIEEIISAAKQLRSRGIENV
                     IISMGEQGSLWLNHEGVLQARPPRCENVVSTVGAGDSMVASLIYGLSQGWSKEQTLRF
                     ASATSALAVVQSNVGIADRAALDDIIKQTDITVISENH"
     gene            2641..4305
                     /locus_tag="PXB27_001689"
     CDS             2641..4305
                     /locus_tag="PXB27_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL5346455.1"
                     /translation="MNISFVFPQTLGNARRFLVNEMLSAAAKQQGHNVVAANQADFVV
                     LFDNAIPQEAAGKQGAILDLEQAFAQPEASLKQAVENAQTFANATATTAVSAVSSGVK
                     NIVAVTACPTGVAHTFMSAEAIENYAKAQGWNIKVETRGQVGAGNPISAEEVAAADLV
                     FVAADIDVDLEKFKGKPMYRTSTGLALKKTTQEFEKAFAQAKIYEGGVAKADNAEAPT
                     SEKKGLYKHLMTGVSHMLPLVVAGGLLIAISFMFGIEAFKDETIAGGLPKALMDIGGG
                     AAFHLMIAVFAGYVAFSIADRPGLAVGLIGGMLATTAGAGILGGIVAGFLAGYTVKFL
                     NGAIQLPASLTSLKPILILPLLGSAIVGLLMIYVINPPVKALMDALVEWLNTMGQTNA
                     IILGIILGAMMCTDMGGPVNKAAYTFGVGLIASQQYMPMAAVMAAGMVPPIGMAIATL
                     IARSKFNTNQRDAGKASFVLGLCFISEGALPFVAADPVRVIASSILGGATAGAISMAL
                     GITLQAPHGGLFVIPFVSQPLMYLAAIAIGSLVTGVVYAAIKQKAE"
     gene            4472..4990
                     /locus_tag="PXB27_001690"
     CDS             4472..4990
                     /locus_tag="PXB27_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lhr family helicase"
                     /protein_id="HDL5346456.1"
                     /translation="MILLRGDTMQFIEFTDPDSGISYQYSEFTIANVAFIINFCSDAD
                     VISTLSALGKDITNYINTYSCCTIKFMAKEHLENSGSNIDIYAPAANHQFKRKEIIAL
                     QETLERLLFEHYVRFTPESYLFIAERDSLNRMYQRMCVPRCDFMQSFQVVYPLGVNQD
                     CFILITPKGNLK"
     gene            complement(5143..6006)
                     /gene="rarD"
                     /locus_tag="PXB27_001691"
     CDS             complement(5143..6006)
                     /gene="rarD"
                     /locus_tag="PXB27_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDL5346457.1"
                     /translation="MLKGILASLAANVLFGAVYYFAILLRPLEGEAMFGFRIVVVIPF
                     ILLAIILFKQQSAFADLLQKIRQNPPLVLVILLLALNTGVQLWLFLWAPNHGQALQVS
                     FGYLLLPIVAVALGKMVFKEHFTRLKWLSLLFAVIGVGSSLFFNSTISWATWVAGLGY
                     PLYITLRRYFQINTLATFFVELLLVLPVALYYLSQVEMTPIVAENPQIYFYLGLLGLV
                     SGSAFILYILSSNLLPINVLGLLGYVEPLVMLLISFAIGEKLDANSYILMVCLLVSIG
                     LLSWDSFRSKV"
     gene            6184..6768
                     /locus_tag="PXB27_001692"
     CDS             6184..6768
                     /locus_tag="PXB27_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HDL5346458.1"
                     /translation="MKKIFWILLIAMLAVGGYSLTKYNDLMRAEEDINSVWGNVESAY
                     QRRADLIPNLVNTVKGQANFEKETLTSVIEARAKATAVTIDPSNATEEQMAKFQEAQQ
                     GVNSALSRLLVSVEKYPELKAHEAFLNLQAQLEGTENRINVERNNFNEKVKEYNKQVR
                     EFPTKLAAMIMGFKAKPQFKSEAGSDKAPTVNFN"
     gene            6850..7308
                     /locus_tag="PXB27_001693"
     CDS             6850..7308
                     /locus_tag="PXB27_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDL5346459.1"
                     /translation="MGLFSFLSNKLPIDTQRIEQAIADLEQKTSAELRVVVERKAKIG
                     KTDNAAMLRASELFDELKMRETAEHNGVLIYLSFKPHYAAIIGDEGIHQKVDNDFWQK
                     IYATMCLDCQQGDYTQAICDAIKMVEAPLAHHFPYQADDKNELSNEVVVK"
     gene            7305..8162
                     /locus_tag="PXB27_001694"
     CDS             7305..8162
                     /locus_tag="PXB27_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDL5346460.1"
                     /translation="MMKSLCKFLSIFNRLLLVCLALFSLNATAVTYPEPPNPFYYITD
                     YTKNTLSQQEWRTLEDALIANCAKTSSQIAVVIIPSTEGEAVSTYAHTLFNKWGIGRS
                     KNNENNGILLLIAKNDRKLFIATGRGLEGALPDAIASSIIRNDITPFFKQEQYAAGIA
                     HGLSAIMAAINGEYAPYASYGEEMQEFDDIDGLLFFLFAGIVIFLIFRPRGNSTYISP
                     SSMDQLGQILRESQRRGGGFGGGLGGGFDRGSGGFGGGFGGGFGGSRGSGDSFGGGSS
                     GGGGAGGSW"
     gene            complement(8277..8756)
                     /gene="ispF"
                     /locus_tag="PXB27_001695"
     CDS             complement(8277..8756)
                     /gene="ispF"
                     /locus_tag="PXB27_001695"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HDL5346461.1"
                     /translation="MIRIGHGFDVHAFGEDRPLMIAGVEVPYHTGFIAHSDGDVALHA
                     LTDALLGAAALGDIGKLFPDTDMQYKNADSRGLLIEAYRQVQEQGYQVGNVDVTIIAQ
                     APKMRPHIDKMRQVIADDLQCDISQVNVKATTTEKLGFTGRGEGIACEAVALLLKRA"
     gene            complement(8758..9441)
                     /gene="ispD"
                     /locus_tag="PXB27_001696"
     CDS             complement(8758..9441)
                     /gene="ispD"
                     /locus_tag="PXB27_001696"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDL5346462.1"
                     /translation="MKRKIIAVIPASGVGSRMQAEKPKQYLQLKGKTILEHTVSIFLE
                     HPHVEKVIVAVARKDPFYSTLELLKSPKIQLVFGGETRADSVLNALQEINTENAWALV
                     HDAARPCLKRSDLDKLLQINDPQGGILAIPAVDTMKRANGTKIAHTEDRSTLWHALTP
                     QFFPAQALKQALSSAFANKLQVTDEASAMELAGYQPQLVSGRSDNIKITRPEDLALAE
                     FYLTHNLEN"
     gene            complement(9459..9737)
                     /gene="ftsB"
                     /locus_tag="PXB27_001697"
     CDS             complement(9459..9737)
                     /gene="ftsB"
                     /locus_tag="PXB27_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HDL5346463.1"
                     /translation="MRLLITFFALLLGFFQYSFWFGKNGWSDYQEVQEEIALLKVENT
                     KLTSRNKLIEAEIYDLKNGVDALEERARLEREMVKENEMFYRIVPKNK"
BASE COUNT         2948 a         2100 c         2039 g         2711 t
ORIGIN      
        1 aaataaaatc gttttaatct ttagtttaaa tcatcaaacc ttaaattttg tgacatagat
       61 cacattttga aacattttta tggatatttt tagttggcta taacggttta gcaaaatata
      121 gagtaaacta accataacat tacagtttaa aaatcaactt taaatccaag gagctaaaat
      181 gcttaattta tcggcaaaaa acatccgttt aaatggttca gcaagcaaca aagaagaagt
      241 tatccgtttt gttgccgccg aattagtcgc caagggcaat gtagcagccg gctatgaagt
      301 cggtatgctt aatcgtgaaa cccaaacctc tactttttta ggtaacggaa ttgctattcc
      361 ccacggcacg cttgataccc gtgatttagt caaagaaacc ggcgtgcaaa ttatccaact
      421 tccgcaaggc gtggaatggg gcgaaggcaa cactgcttat gtagttatcg ggattgcggc
      481 taaatcggac gaacacttaa ccttactccg tcaactcacc actgtattaa gtgatgaaga
      541 ggctgctgcc acattagcga aaacgcaaaa tttagatgaa tttattgccg tattaagcgg
      601 taagaaaaac cttcctgcct tatctcaaga gctgatttct ctgaatatcg aatcttcaag
      661 ccctatcact ttatcagcca ttaacgccgc aaaactacaa gagcaaggct atgtttccca
      721 agcattcatc agcgatgtgg ttgccaacaa agcgttacat ttaggcaata atctatttgt
      781 ggtggattcc gccaaaggca accaagctaa cggtatcgct attgcccgta atacgcaagg
      841 tcaaacccta ctaactgtgg cagaaaccga caatgctcta cagccactat taactcaatt
      901 attaaaacca gaagtaagaa gccagtttgc tcatttctct gcggagcaaa tgcttggttt
      961 attaaaaggc gaaagcgtag cttcaacccc tgtaaccaat gtcgaagcga gtggcaacca
     1021 agtggttggc acattcacca ttcgtaacga caacggctta cacgcccgcc cggctgcggt
     1081 gttagtacaa acggtgaaac cgttctcctc aaaaatcagc gttgaaaatt tagaccgcaa
     1141 cactgcaccg gcaagtgcga aaagtgcaat gaaagtagta gcactagggg caagccaagg
     1201 gcatcgttta cgctttgtgg cagagggaga agacgcccaa caagcgattg aggcattaca
     1261 acaagcaatt attaacggct taggcgaaag tgtttcattc gtaccggcgg tggcagatac
     1321 gattgaaagc attgctgttt cagcaattcc tgcggaagaa actgcgactg ctgtcgatgc
     1381 caatacgaaa gaagcaactt ttgtaattaa aaacgaacac ggtttacacg ctcgcccaag
     1441 tgcggtgttg gtgaatgaag tgaaaaaatt caacgccaaa attgaagtgc aaaatgttga
     1501 taaaaattca ccgcttgtaa gtgccaaaag cttaatgaaa attgttgccc ttggcgtaac
     1561 caaaggcacg acgctacgct ttgtggcaag cggtgatgat gccgaagtag cacttgcggc
     1621 aatcggtgct gcgattgaag caggtttagg cgaataagcg aggaatcatt atgcgtattg
     1681 caaccgttac cctcaaccct gcctttgatt tagtcggcaa acttcaacgt attgaaatcg
     1741 gcgaagtgaa caccgtagaa acccttggtt tataccctgc cggcaaaggt attaacgttg
     1801 ctaaagtttt agctgactta ggcacaaaac tttcggttac cggcttttta ggcgaagaaa
     1861 atcaaggcga tttcgtaaat gcctttaaac acaatgattt ggctgatgaa ttttaccgta
     1921 ttaacggtaa aacccgcatt aatgtcaaaa tcactgaaac cgaggctgat gtaactgatc
     1981 tgaactttct cggctttgaa atttcggaaa gcgattggga aaaattcgtc acattatctc
     2041 atcagtggga aagcaaattt gacttagttg cagtgtgtgg atctctccct cgtggcgtaa
     2101 cgccggaaaa atttgcttct tggttggaat ctttacaccg ccaaggctta aaagtggtgt
     2161 tagataccag caatgcggct ctcaccgccg gcttaaccgc tcacccttgg ttagtaaaac
     2221 caaaccgccg agagctggaa gtgctggtcg ggcattcact caactcaatc gaagagatta
     2281 ttagtgcagc taaacaatta cgttcccgtg gtattgaaaa tgtaattata tcaatgggcg
     2341 aacaaggctc tctttggcta aatcacgaag gcgtgttaca agcccgccca ccacgttgcg
     2401 aaaatgtagt tagcactgta ggtgctggtg attcaatggt tgccagcttg atctacggct
     2461 tatctcaagg ttggagcaaa gagcagacct tgcgttttgc ctcagctact tctgctctag
     2521 ctgttgttca aagtaacgtt gggattgcag accgtgcggc gttagacgat attattaaac
     2581 aaaccgacat taccgtgatt tcagaaaatc attaaacctc aatcaaagga acaaaccaat
     2641 atgaatattt cttttgtatt tcctcaaaca ctaggcaacg cacgccgttt cttagtgaat
     2701 gaaatgttat ctgccgctgc caagcaacaa ggacacaatg ttgtggcggc aaaccaagct
     2761 gattttgtgg tgttattcga taacgcaatt ccacaagaag ctgccggcaa gcaaggggca
     2821 attttggact tagaacaagc ctttgctcaa cctgaagcca gtctcaaaca agcggtcgaa
     2881 aatgcacaaa cttttgcaaa tgctaccgca accactgcgg tttcagcggt ttcatctggt
     2941 gtgaaaaata tcgttgctgt taccgcttgc ccaactggcg tggctcatac ttttatgtct
     3001 gcggaagcaa tcgaaaatta tgccaaagca cagggttgga acatcaaggt tgaaacccgt
     3061 ggtcaagtag gagcaggaaa tccaatttcc gccgaagaag tcgcagcggc agatttagtc
     3121 tttgtagctg ccgatattga tgtcgatttg gaaaaattca aaggcaagcc gatgtatcgc
     3181 acctcaaccg gtttagcatt gaagaaaacc actcaagaat ttgaaaaagc ctttgctcaa
     3241 gcgaaaattt acgaaggtgg tgtggcaaaa gcggataatg ctgaagctcc aacttctgag
     3301 aaaaaagggt tatataaaca cttgatgacc ggtgtatcac atatgttacc gctggtggta
     3361 gcaggtggct tgctcatcgc tatttccttt atgttcggaa ttgaagcctt taaagatgaa
     3421 accatcgcag gcggcttgcc gaaagcctta atggatattg gtggcggtgc ggcattccac
     3481 ttaatgattg cggtattcgc aggctatgta gcgttctcaa ttgcagaccg tccggggtta
     3541 gctgtcggtc ttatcggcgg tatgcttgct accaccgcag gagcagggat tttaggtggt
     3601 atcgtagccg gtttcttagc cggttatacc gtaaaattct taaatggtgc gattcagcta
     3661 ccggcaagtt taacctcact taaacctatt ttgattttac cactcttagg ctcagcgatt
     3721 gtcggcttac tgatgattta tgtcatcaac ccaccggtga aagcattaat ggatgcctta
     3781 gtggaatggt taaacacaat ggggcaaacc aatgcaatta ttttaggtat tattctcggt
     3841 gcgatgatgt gtaccgatat gggtggtccg gtgaataaag cggcttatac ctttggtgtg
     3901 ggtttaattg cttcacaaca atatatgcca atggcagcgg tgatggcggc aggtatggtt
     3961 ccaccaattg gtatggcgat tgcgaccctg attgctcgta gcaaattcaa caccaaccaa
     4021 cgtgatgcag gtaaagcttc attcgtgtta ggtttatgct ttatttcaga aggggcatta
     4081 ccgttcgtgg cggcagaccc tgtgcgtgta attgccagct caatcttagg cggtgcaacc
     4141 gccggtgcaa tttcaatggc actaggcatt accctacaag ccccacacgg cggtttattc
     4201 gtgattccat tcgtttcaca accgttaatg tacttagctg ctattgccat cggctcatta
     4261 gtcaccggcg tagtctatgc cgctatcaaa caaaaagcag aataaagcac aaacacaaat
     4321 acaaacaaaa acggagcgaa acgctccgtt tttatatcat attggtactt actgaattct
     4381 atttcatcga aatatcaagt ctaaaatatt atttttgcga tcgagatcga aaaaatcatt
     4441 ttcatcactt tacgaataat atcaactcgt tatgattttg ctacgaggag atactatgca
     4501 gtttattgaa tttaccgatc ctgatagtgg aatttcttat caatacagtg aatttactat
     4561 tgctaatgtc gcatttatca ttaatttctg ctcagatgcc gatgttatat cgactttgtc
     4621 cgcattaggc aaagatatca caaactatat aaacacctat tcctgttgca caatcaaatt
     4681 tatggcgaaa gagcatctag aaaatagcgg ttctaatatt gacatttatg cccccgcagc
     4741 gaatcatcag ttcaaacgta aagaaataat tgctttacaa gaaacccttg agcgtttatt
     4801 atttgagcat tatgtacgtt ttacaccaga aagctatctc ttcattgccg agagagattc
     4861 acttaaccga atgtatcagc gtatgtgtgt gccacgatgt gattttatgc aatctttcca
     4921 agtggtttat cccttgggcg ttaatcaaga ttgtttcatt ttaattacgc caaaaggaaa
     4981 tctaaaatga atcaacaacg tccagtttcc gttcgtttaa agcaagaagg cttaatcgct
     5041 tttaagcaag cctatcagcg agcaaaaaaa gcaggtctat tgaaaccggt caaataatct
     5101 ttaaaccgct tgctctacaa gcgatttatt tttccttaat tatcacacct tgctacgaaa
     5161 actatcccaa ctcaataatc caatcgaaac cagcaaacag accatcaaaa tataggaatt
     5221 ggcatcgagt ttttcgccga tggcaaaaga aatcagcaac atcaccaacg gttcaacata
     5281 gcccagtaaa cctaatacat taatcggtaa taaattgctc gagaggatat aaagaataaa
     5341 agcactgcca cttaccagcc ccagcaatcc gaggtagaaa taaatttgcg gattttctgc
     5401 cactattggc gtcatttcca cttggctgag ataataaagt gcaacgggta ataccagtaa
     5461 caactccaca aaaaaagtag ctaacgtgtt gatttgaaaa taacggcgaa gggtaatata
     5521 aagcggataa cccaacccag ctacccaagt cgcccacgaa atcgtgctgt taaaaaacaa
     5581 actactgcca acaccgatga ctgcgaaaag tagagaaagc catttcaggc gggtaaaatg
     5641 ttctttaaac accatttttc ctagagccac cgccacaatc ggtaaaagca agtaaccaaa
     5701 cgacacctgc aacgcctgcc cgtgattagg agcccataaa aacagccaaa gctgcacgcc
     5761 ggtgttgaga gcaaggagca aaatgaccaa tacaagcggt ggattttgtc gaattttttg
     5821 caaaagatcc gcaaaagccg attgctgttt aaacaatata atcgccagca aaataaacgg
     5881 aatgactacc actatccgaa agccgaacat cgcctctccc tctaacgggc gaagcaatat
     5941 cgcaaaatag taaactgctc caaacagcac attggctgca agcgaagcta aaattccttt
     6001 gagcatttgg ctttcctctt atcaacaatc aaacaacaag cggttgactt tgaacaaaaa
     6061 tttacaaatc ttatctacgc cccatagtat gaaaaaattt gtaagtaagc tagctatatt
     6121 cgatttttcc cgatacaatg ctcccaattt ttcactcttt tatttatatt ataggacttc
     6181 ataatgaaaa agattttttg gattttactg atcgccatgc ttgctgttgg cggttattct
     6241 ttaacaaaat acaacgattt aatgcgtgca gaagaagaca tcaactccgt atggggaaat
     6301 gttgaatcgg cttaccaacg ccgtgcggat ttaattccaa acttagtgaa taccgtaaaa
     6361 ggtcaggcta actttgagaa agaaacttta acctctgtga ttgaagctcg tgcaaaagca
     6421 actgcggtaa ccattgaccc aagcaatgca actgaagagc aaatggctaa attccaagaa
     6481 gcccaacaag gtgtaaactc tgctctttca cgcttattgg tttcagttga gaaataccct
     6541 gaattaaaag cccacgaagc gtttttaaat ctacaagctc aattagaagg tacggaaaac
     6601 cgcattaacg ttgaacgcaa taactttaac gaaaaagtga aagagtacaa caagcaagtg
     6661 cgtgaattcc cgactaaatt agcggcaatg attatgggct ttaaagcaaa accgcaattt
     6721 aaatctgaag caggttctga taaagcacca accgtaaact tcaactaatt ttagtttcaa
     6781 tttattcata ttaaaagcaa taagctcttt caaatccggt gcttattgct ttatttttaa
     6841 ggggaacaaa tgggattatt ttcttttctc tccaacaaat taccgataga tacgcaacgc
     6901 attgaacaag caattgccga tttagagcaa aaaacctctg ccgaattgcg tgtggtggtg
     6961 gaacgaaaag ccaaaatcgg caaaaccgac aacgccgcta tgttgcgagc aagtgagctt
     7021 ttcgatgaat taaaaatgcg tgaaacggct gaacataacg gtgtgttgat ctacctttca
     7081 ttcaagcctc actacgctgc gattattggc gatgaaggca ttcatcaaaa agtcgataac
     7141 gatttttggc aaaaaatcta tgccacaatg tgtttggact gccaacaagg cgattacacc
     7201 caagccattt gtgatgcaat caaaatggtg gaagctccgc tcgctcacca tttcccttat
     7261 caggctgatg ataaaaacga gctttcaaac gaggtggtag tcaaatgatg aaatcccttt
     7321 gcaaatttct atctattttc aaccgcttgt tattggtgtg cttggctctg tttagcctaa
     7381 atgccacagc ggtgacctac ccagagccgc ctaatccgtt ttattatatt acggactaca
     7441 ccaaaaacac cttgagccaa caagaatggc ggacattaga agatgcctta attgctaact
     7501 gtgccaagac aagttcgcaa attgccgtgg tgattattcc aagcacggaa ggcgaggcag
     7561 tttcaaccta tgctcatacc ctgtttaaca aatggggaat cggacggagc aaaaataacg
     7621 agaacaatgg tatattgtta ttaattgcca aaaatgatcg caaactcttt attgccaccg
     7681 gacgtgggct tgaaggagcg ttacctgatg cgattgcatc gagcattatc cgcaatgaca
     7741 tcacgccatt cttcaaacaa gagcaatatg cagccggtat cgcccatggc ttatccgcca
     7801 ttatggcagc cattaacggg gaatatgccc cttatgcaag ttatggcgaa gaaatgcaag
     7861 aatttgatga catagacggc ttactctttt tcttgtttgc cggcattgtg attttcctaa
     7921 ttttccgccc acgaggaaac agtacctata ttagtcccag ctcaatggat caactaggac
     7981 aaatactaag agaaagccaa cgcagaggtg ggggctttgg cggtggtctc ggaggtggct
     8041 ttgatcgagg ctcaggtgga tttggcggag gcttcggtgg tggttttggc ggaagcagag
     8101 gcagtggcga cagcttcggt ggtggttcat caggcggtgg aggcgctgga ggaagctggt
     8161 agtttccgcc caatttaaaa caatacaagc ggtcatattt ctcaaaaaaa ttgcgtattt
     8221 ttcgctaaac gcacaaaacg ctaatgaaaa taagaccgct tcctcacttc tctaagctat
     8281 gcccttttta gtaacaatgc tacggcttca caagctatgc cttcacctcg cccggtaaac
     8341 cctaattttt ctgttgtagt ggcttttaca ttcacttgtg atatatcgca ttgtagatcg
     8401 tctgcgatca cctgtctcat tttatcaata tgcggtcgca tcttcggagc ttgagcaata
     8461 atggttacat cgacgttacc gacttgatag ccctgttctt gcacttgtcg gtaagcttca
     8521 atcaacaaac cacgactatc ggcatttttg tattgcatat cagtatcagg gaataatttg
     8581 ccaatatcac ctaaagctgc tgcgccaagc aacgcatctg ttaaagcgtg cagcgctaca
     8641 tctccgtcac tatgcgcaat gaagcctgta tgataaggca cttcaacccc agcaatcatc
     8701 agtggtctat cttcgccaaa agcgtgaaca tcaaaaccgt gtccaatacg tatcatttta
     8761 gttctccaaa ttatgtgtaa ggtaaaattc cgctaaggct aaatcttcag ggcgagtgat
     8821 tttgatatta tcacttctac ctgaaaccaa ttgtggctgg tagcctgcaa gctccattgc
     8881 agatgcttcg tctgttacct gaagcttatt ggcaaaagca gaggataatg cttgttttaa
     8941 tgcttgagca ggaaaaaatt gtggggttaa agcgtgccat aaggttgagc gatcttctgt
     9001 gtgtgcgatt ttcgtgccgt ttgctctttt catcgtatct accgctggaa tggcgagaat
     9061 gccaccttgc ggatcgttaa tttgcaaaag tttatctaaa tctgaccgct tgaggcaagg
     9121 gcgagccgca tcgtgaacta atgcccaagc attttcagtg ttgatttcct gtaaggcatt
     9181 tagcactgaa tctgctctcg tttctcctcc aaaaacaagt tggatcttag gggattttag
     9241 gagttccaat gtggaataaa aagggtcttt tcttgccacc gctacaatca ctttttcaac
     9301 atgagggtgt tcaagaaaaa tagacactgt atgctctaag atagtcttac cctttaattg
     9361 taaatattgt tttggttttt ctgcttgcat ccggctacca actcctgacg ccggaattac
     9421 tgcaataatt tttcgcttca taatatatca attatgtatt atttgttttt tggtacaatt
     9481 cggtagaaca tctcgttctc ttttaccatt tctcgttcaa gtcttgctct ctcttcaagt
     9541 gcatctacgc cgttctttaa atcgtaaatt tcggcttcaa ttagtttatt gcgtgaagta
     9601 agctttgtgt tttctacttt taataaagca atttcttctt gcacttcttg ataatcactc
     9661 caaccatttt ttccaaacca aaagctatat tggaaaaagc cgagtaatag ggcaaaaaaa
     9721 gtaattaata aacgcattta ttttctctta tatgaaatag taatgaacta agcgggttga
     9781 ttttaccttg aatttgca
//
LOCUS       DANLUQ010000092         9758 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.092, whole genome shotgun sequence.
ACCESSION   DANLUQ010000092 DANLUQ010000000
VERSION     DANLUQ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9758
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.092"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..780)
                     /locus_tag="PXB27_001698"
     CDS             complement(<1..780)
                     /locus_tag="PXB27_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346464.1"
                     /translation="MAKQIFKQAPLPFVGQKRMFLAQVSQILNENITDDGQGWTIIDV
                     FGGSGLLAHTAKHIKPKAHIIYNDYDGYAERLKHIPDTNRLRKQIYDIIGKSTPKNKR
                     LDPDKKSQVINIIQSFDGYIDVNCVASWLLFSGQQINSLEDLFNKIFWNGVRQTDYPS
                     AEGYLDGIEVTHESFHKLLPRFQHKDKVLLLLDPPYLCTRQESYKQATYFDLIDFLRL
                     INLTKVPYIFFSSTKSEFVRFVDFMVSEKKDNWQTFENAKWI"
     gene            complement(827..952)
                     /locus_tag="PXB27_001699"
     CDS             complement(827..952)
                     /locus_tag="PXB27_001699"
                     /inference="COORDINATES: protein motif:HMM:NF021626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL5346465.1"
                     /translation="MQSLRLIEIRCKYCQKLLAKAKDVQHLEIKCVRCKSINKFN"
     gene            complement(1099..1308)
                     /locus_tag="PXB27_001700"
     CDS             complement(1099..1308)
                     /locus_tag="PXB27_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346466.1"
                     /translation="MFRNELQVIDGKRYIVLECQFRREWRVAIESRGTVTNGEAIEIC
                     QYWVKYKGVKPEQLKIVEVPDILKE"
     gene            complement(1311..1604)
                     /locus_tag="PXB27_001701"
     CDS             complement(1311..1604)
                     /locus_tag="PXB27_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346467.1"
                     /translation="MSESFSLEKALWEEPRPRLQLDLPCPVIKLEVGKRYYTINIHGV
                     DCYDGDMSFTEIDEWTYESIDDVQTKSRAEQGLLFASEADAQEWIDAMRNSSR"
     gene            complement(1597..2034)
                     /locus_tag="PXB27_001702"
     CDS             complement(1597..2034)
                     /locus_tag="PXB27_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346468.1"
                     /translation="MKTTQHILDEREQQHGNYDSFAKIYGGLRKVSDSHAEKLTWPQQ
                     IAVEMMLFKIARILNNGANHQDNYQDIAGYAMLGGGLYNPNVSAEVKALPKPLTDSIY
                     PESHLNKSSVWRLDLEFETKEEATEVLEALTGKSAGVSEVNNE"
     gene            complement(2273..2950)
                     /locus_tag="PXB27_001703"
     CDS             complement(2273..2950)
                     /locus_tag="PXB27_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein/antirepressor Ant"
                     /protein_id="HDL5346469.1"
                     /translation="MSNQVQFPVFNFNSSAVRVIIDPNQEPWFCGADVCRILGYVNES
                     LTLQKHCKENGVSKRYLTDKMQRQQKAIFINEPNLYRLIIKSRKPEAEKFEAWVFEEV
                     LPQIRKTGKYALQNNQQNLPLAPPPKKYSFDFTEDELQSLIWAWFAFVRGIHTFRYIY
                     PMFQKLGSNIAPEIYGQGFEYSHTAQSAHKILERITREFDCDPMTNWRVLKHLRGFDP
                     AFKKPTF"
     gene            complement(3198..4037)
                     /locus_tag="PXB27_001704"
     CDS             complement(3198..4037)
                     /locus_tag="PXB27_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HDL5346470.1"
                     /translation="MANSVMIVQGVEVKVTARDGEDYISLTDMCKAFGDSDQLIKNWL
                     QNKNTIEFLQVWEELNNPNFNLVELHQIKNNIGLNRFVMSVKKWTATGAIGLVAKAGR
                     YGSGTYAHKDIALEFGSWLSPEFKLYLIKEFQRLKQQEANESKLEWSVKRILSKANYR
                     IHTDAIKAHIIPALLNTKQHTFVYSSEADILNQALFGQTAKQWKDRNPNLKGNMRDHA
                     TVEQLTVLAALESQNALLIEQGYSQEERLAMLNRLAIQQMSSLLQTRAIEELKEKPLL
                     IEE"
     gene            4267..4644
                     /locus_tag="PXB27_001705"
     CDS             4267..4644
                     /locus_tag="PXB27_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346471.1"
                     /translation="MFSLLRDALTFAAMFTGILFPAIIHKYDLSLGTQIQLLSFVPAF
                     FVLCHFLIKRKYQRDFDRLSYLSAVVCVAMCAGVGAFSQSEMIELGFKVERVLDYVTI
                     KFLLYFWSLLLLPVVLRKLFGKD"
     gene            4776..4964
                     /locus_tag="PXB27_001706"
     CDS             4776..4964
                     /locus_tag="PXB27_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346472.1"
                     /translation="MKELFKQWLINQDSPFINSCGVECILSKVDDWLNIINANEEETE
                     TLVEWLNAFLQEVSVFVA"
     gene            complement(4968..5465)
                     /locus_tag="PXB27_001707"
     CDS             complement(4968..5465)
                     /locus_tag="PXB27_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HDL5346473.1"
                     /translation="MQQLIKNIEQWAEDRNLINGSTPQKQMLKLMEEFGELCAGVATN
                     EFETILDSLGDCLVICTIMRKQLKMEKLFSISHCDNTNFNCGVAAQIVQANIDLGNVA
                     SELSRGCDLSRLEDYLSGFENQLDLIAQFYKTDVQTALLYAYIQIKNRKGKMIDGVFV
                     KAEDL"
     gene            complement(5449..5649)
                     /locus_tag="PXB27_001708"
     CDS             complement(5449..5649)
                     /locus_tag="PXB27_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346474.1"
                     /translation="MTDTKRLDFIKKHKMEIRYNGLSDEFVISNHAENSQHFRMARNA
                     DLRTAIDEAMKEMRGLNNATTN"
     gene            complement(5646..6164)
                     /locus_tag="PXB27_001709"
     CDS             complement(5646..6164)
                     /locus_tag="PXB27_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HDL5346475.1"
                     /translation="MNEDKFFSVDVSNDIHILNLHETLEQAKQSCLNGATEAYEFAGD
                     MDDHESYEAYDLPYAVYGVVLGRAKSDIRPLTDEERESELFGEVEQVIEPPTLVENNG
                     WISIEDKLPPIETDVLGLCDISGMQLILIVSRELADNEWYFLSVNQYGLDDDVIAVTH
                     WQPLPKLPKDNQ"
     gene            complement(6274..7077)
                     /locus_tag="PXB27_001710"
     CDS             complement(6274..7077)
                     /locus_tag="PXB27_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HDL5346476.1"
                     /translation="MDKSTIQQISTLAVAATKEVQTDFGTVMLPEGIKLQNLEPFQAH
                     RNQFRAAFSTQRFGSLIEYATANAQENAQCFIDQEKMSAEIVFDMGNREQAGHAKHRA
                     NLVMKKTAAYKALCEINGSRRSQRDFSDFLEDWGGYLTAYHHEDEISIKNAVQAVRKM
                     TIDYARNEEHELSDFAAKKSAMESVEAKSTLQLPTHLVFTCNPYNGLDTRSFTLRVQV
                     LTGSGEPVLTARLVQAEQIEEAIATEFAEKLSDALSETSIKVNIGTIEI"
     gene            complement(7147..7506)
                     /locus_tag="PXB27_001711"
     CDS             complement(7147..7506)
                     /locus_tag="PXB27_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346477.1"
                     /translation="MAKTNVPEFLDELDCGIFKDKLATALSEVALGVLTHDKKGKVTV
                     EFSLDKMDSDSPSVQIQHKLSYVKPTKRGKSAEEDTTATPMYVHKGGALSATPEKTET
                     AVKESPSGLKALSKAAA"
     gene            complement(7542..8000)
                     /gene="ssb"
                     /locus_tag="PXB27_001712"
     CDS             complement(7542..8000)
                     /gene="ssb"
                     /locus_tag="PXB27_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL5346478.1"
                     /translation="MAGINKVIIVGRLGNDPELRTMPNGDTTAKISVATSTEWTDKVS
                     GDKKQATEWHSIIAFRNLADIIGKYLKKGSQVYVEGKLRTRKWQAQDGTDRWTTEIIA
                     DQLQMLGSSSSGNSNNNWATEPAGNPPPTNPYNHVMTDSESRDFDDDIPF"
     gene            complement(8003..8200)
                     /locus_tag="PXB27_001713"
     CDS             complement(8003..8200)
                     /locus_tag="PXB27_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346479.1"
                     /translation="MARKIDAGVEIVEVDKLFIAKFFYDGKLQHTTYPQYSRQNAIML
                     INRKIERFNVGRDKPIKLYKE"
     gene            complement(8184..8369)
                     /locus_tag="PXB27_001714"
     CDS             complement(8184..8369)
                     /locus_tag="PXB27_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346480.1"
                     /translation="MNKMKQLAPFALLILLLGIVGRMDYDDHIQMQRYKCERNQGVWQ
                     VESNGNQYCGGKYGTKN"
     gene            8583..8858
                     /locus_tag="PXB27_001715"
     CDS             8583..8858
                     /locus_tag="PXB27_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346481.1"
                     /translation="MKNNILITYDLNKSGQNYAALIEKIKTLGAWAKVQQSAWYLHTS
                     YSSKEVLEMLSTVTDYNDSLFVAQLSEANWRGLSNEVSKFIQEQWWK"
     gene            complement(8861..9112)
                     /locus_tag="PXB27_001716"
     CDS             complement(8861..9112)
                     /locus_tag="PXB27_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346482.1"
                     /translation="MKNKNFEIAKVVILNEQLGSPKDLELKVVENGLSDEDKYHIKQA
                     VLKAVENGCLDPEMLASRCCSAIETINKSGINTGNGSCE"
     gene            complement(9565..>9758)
                     /locus_tag="PXB27_001717"
     CDS             complement(9565..>9758)
                     /locus_tag="PXB27_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346483.1"
                     /translation="ILCGRKEKRTSDFINVFEVFENETTQEFVIERAMFRNGKLIDWN
                     QSDKMNAEQAQQLWQAYIH"
BASE COUNT         2576 a         1967 c         1880 g         3335 t
ORIGIN      
        1 aatccatttc gcattttcaa aagtttgcca gttatctttt ttctcgctaa ccataaaatc
       61 tacgaacctc acaaattcac tttttgttga actaaaaaag atataaggca ctttagttaa
      121 attgattaat cttaaaaaat cgattaaatc aaaataagtg gcttgcttat agctttcttg
      181 gcgagtacaa agatatggcg gatcaagtaa taacaatacc ttatctttat gctgaaaacg
      241 tggtaatagt ttatgaaaac tttcgtgagt aacttctatt ccatctaaat agccttccgc
      301 acttggataa tcggtttggc gaacaccatt ccaaaatatt ttgttaaata gatcctctaa
      361 actattaatt tgctgtccgc taaatagcaa ccaagaagcc acacaattta catcaatata
      421 cccatcaaaa gactgaataa tattgataac ttgagatttc ttatcaggat ctaatcgctt
      481 gtttttaggc gtactcttcc caataatgtc ataaatttgc ttgcgtagcc tatttgtatc
      541 gggaatatgc tttagccgct ccgcatatcc atcataatca ttgtagatga tgtgagcttt
      601 tggtttgata tgtttggcag tatgagctaa taacccacta ccaccaaata catctatgat
      661 tgtccaacct tgtccgtcat cagtaatatt ctcatttaaa atttgagaaa cctgagctag
      721 aaacattcgt ttctgaccta caaacggaag tggggcttgt ttaaagattt gctttgccat
      781 agttctccta tggcgttcag gcgttcttaa cgctcagaca ctcaattcag ttaaatttat
      841 tgattgattt acaacgtaca catttaattt ctaaatgttg tacatctttc gcttttgcca
      901 ataatttttg gcaatactta cagcggatct caattaatct taatgattgc atataaatac
      961 acttttactt cattttttga taaaattccg cctacctagc taggtggcgg cgtatggcta
     1021 tatgcaagcg tgctttgcgt agctggcatt ttaagtgtta ccgcacttat aatgtcgccg
     1081 tttttaactc atttgtcctt attctttcag aatatctggc acttcaacaa ttttcagttg
     1141 ctcaggtttt actcctttat atttaaccca atattggcag atttcgattg cttctccatt
     1201 cgttacagta cctctagact ctattgcaac tctccattcg cggcgaaatt ggcattcaag
     1261 tacgatataa cgtttaccat ctattacttg gagttcattt ctaaacatag ctacctacta
     1321 ctatttcgca ttgcatctat ccattcttga gcatctgctt cactggcaaa tagtaaccct
     1381 tgttcagctc tgctttttgt ctgcacatca tcaatacttt cataagtcca ttcatcaatt
     1441 tcggtgaagc tcatatctcc atcataacag tcaacgccat gaatattgat tgtataatag
     1501 cgtttaccaa cttcaagttt tataacaggg caaggtaaat ctaattgcaa tcttgggcgt
     1561 ggttcttccc acagtgcttt ttctaaacta aatgactcac tcattattta cctcactaac
     1621 accggcactt ttaccagtta aagcttccaa cacctcagtt gcctcttctt tggtctcaaa
     1681 ctccaaatcc agccgccaaa cagagctttt attaaggtgt gattcggggt aaatactatc
     1741 ggttaatggt tttggtaatg ctttaacttc tgccgacaca tttgggttat aaagtcctcc
     1801 acctaacatt gcataaccgg caatgtcttg ataattgtct tgatgatttg ctccgttatt
     1861 taaaattcgg gcaattttga ataacatcat ctcaacggcg atttgctgtg gccaagtgag
     1921 cttttctgcg tgtgagtcgc tgactttgcg taaaccacca taaatcttgg caaaactgtc
     1981 gtagttgccg tgttgttgtt cacgttcatc taaaatgtgt tgagttgttt tcatcgtgaa
     2041 ttttccttta gaaatagccg cttgcggaac aagcggttgg gttaattagc gttgagccat
     2101 acaaattttg ccatcgcagt cgttgcgtaa ctctagatgg tcagcagcaa ctgcaaaggc
     2161 ggttaaaaag agtatgattt ttgcgtattt catcgtctaa tcctcaaaat ttgggtgtgg
     2221 aaatccgccg cttgatgttt cgcaagcggt cgggttttat aattttttgt gattagaacg
     2281 ttggcttttt aaatgccggg tcgaaaccac gaaggtgttt gagtacacgc caattcgtca
     2341 ttgggtcgca atcaaattct cgggtaatcc gttcaaggat tttatgagcc gattgtgcgg
     2401 tgtggctata ttcaaaaccc tgtccgtaga tttcaggtgc gatatttgag ccgagttttt
     2461 gaaacatcgg gtagatatag cggaaagtgt gaatgccacg cacgaaagcg aaccaagccc
     2521 atatgaggct ttggagttca tcttcggtaa agtcgaaaga gtatttcttt ggcggtggtg
     2581 caagcggtag gttttgctga ttattttgca acgcatattt accagtcttg cgaatttgtg
     2641 gcagaacttc ttcaaatacc cacgcttcaa acttttccgc ttctggtttg cgtgatttga
     2701 taatcagtcg gtataggttt ggttcgttga tgaagatcgc tttttgttgt ctctgcattt
     2761 tatcggtgag gtatcgtttc gataccccat tttctttaca gtgtttttgc agagtaaggc
     2821 tttcatttac ataacctaaa attcggcaaa catctgcacc gcaaaaccac ggttcttgat
     2881 ttggatcgat aatgacacga acagcagatg aattgaaatt gaaaacaggg aattgaactt
     2941 gatttgacat tttttgtacc ttttggattt aagtatttaa tccgatcatt aagtgggtga
     3001 tcggggttca acttaccctc caaaagatgg gcggagctta ttcagatgta ttgtatatcg
     3061 ctctctcgcc ccgatcattt cggattaaac ctaaaattta ggtacaaaaa aaccgcttta
     3121 tatcggaggc ggaaaccgct tttggaaagt aagtgtggtt atcttagccg aaagggcggt
     3181 tttttgtcaa ttagatttta ttcttcaatt agcaatggct tttctttcag ttcttcaatc
     3241 gctctggttt gtaacagcga gctcatttgc tgaatagcca aacggttgag cattgccagt
     3301 cgctcttctt ggctgtaacc ttgctcaatt agtagggcgt tttggctttc caatgcagca
     3361 aggacggtaa gttgctcaac ggtagcgtgg tctcgcatat tgcctttcag gttcggattg
     3421 cggtctttcc actgttttgc cgtttgtccg aagagagctt gattgagaat gtccgcttcg
     3481 ctggaataaa cgaaagtatg ttgttttgta ttcagcaatg ccggtatgat gtgagctttg
     3541 atggcatcag tatgaatgcg gtagttcgcc ttgcttaaaa tgcgttttac gctccattcg
     3601 agcttacttt cgttcgcttc ttgctgtttg agtcgttgga actctttgat gagataaagc
     3661 ttaaattctg gactgagcca agaaccaaat tcgagagcaa tatctttgtg ggcgtaagtg
     3721 ccgctaccat atcgtcctgc ttttgctact agcccaatag ctcccgttgc agtccatttt
     3781 ttgacagaca tcacaaagcg gtttaagccg atgttgtttt taatttggtg taattccacc
     3841 aaattaaaat ttggattatt cagctcttcc caaacttgaa gaaattcaat ggtattttta
     3901 ttttgaagcc agtttttaat gagctggtca ctgtctccaa aagctttgca catatcagtt
     3961 aggctgatgt aatcctcacc atcacgagcg gttactttga cctccacgcc ttgtacaatc
     4021 attacgctat ttgccatttc gtttctccgt ttttaggtac aaaaaaagcc gttggtggac
     4081 ggcttgaaag tgcggttatc ttaatccgaa ttgggggcgg tgtcaattag attaatccgt
     4141 ataagtatgg aaccaagtta aagagtagaa ctacaacaca taaccaaaaa atgaaattca
     4201 tatcttgctc agttttgatt ttgtgtaaaa tacgcttaag tttgtttttc atttaggaaa
     4261 cctttaatgt ttagtttatt gcgtgatgct ttaacctttg ctgcaatgtt tacagggatt
     4321 ttatttcctg caattattca taaatacgat ctatcacttg gtacacaaat ccaattactt
     4381 agctttgttc cagctttctt tgtgttgtgc cattttctta ttaagcgaaa atatcaacgt
     4441 gattttgaca gactttcgta tttatctgct gtggtttgcg tagcaatgtg tgcaggcgtt
     4501 ggagcattta gccaatcaga aatgattgag ctaggtttta aagttgagcg tgttcttgat
     4561 tatgtgacta tcaaattctt actctacttt tggagcttgc tgttattgcc agttgtgcta
     4621 agaaaattgt ttgggaaaga ttagttggtt atttaaagag agctatttgt caaacctcaa
     4681 tatctatttt tgcgatctgc atcgaaaaaa taaattttcg ttctgacagt aaaacgcttt
     4741 cgtagtaatc tcataaaaaa cataagggga acactatgaa agagcttttc aaacaatggc
     4801 taatcaatca ggactcgcca ttcattaata gctgtggcgt tgagtgtatt ttgtcaaaag
     4861 ttgatgactg gctaaacata atcaacgcca acgaagaaga aaccgaaacg cttgttgagt
     4921 ggctaaatgc ttttctgcaa gaggtttctg tttttgtggc ttaggtacta taaatcttct
     4981 gctttcacaa acacgccatc aatcattttt ccttttcgat ttttgatttg aatataagca
     5041 tagaggagag ctgtttgtac gtctgttttg tagaattgag cgataagatc aagctgattt
     5101 tcaaagccgc ttaagtaatc ttctaatcga gataaatcac aacctctaga taactcactt
     5161 gctacattac ctaaatcaat attggcctga acaatctgag ccgcaactcc acaattaaaa
     5221 ttggtattat cgcaatggct aatgctaaac agtttttcca ttttgagctg tttacgcata
     5281 atcgtacaaa tcactagaca gtcacctaga ctgtctaaaa tagtttcaaa ttcattggtt
     5341 gctacacctg cacataattc cccaaactct tccattagtt ttaacatttg cttttgtggg
     5401 gtagagccgt taattaaatt gcggtcttcc gcccattgtt cgatgttttt aattagttgt
     5461 tgcattgttt agtcctctca tttctttcat tgcttcatca atcgccgtgc gtaaatcggc
     5521 atttcttgcc atacggaaat gctggctgtt ttcagcgtga ttactaataa caaattcgtc
     5581 tgataagcca ttgtagcgga tttccatttt gtgctttttg ataaaatcta agcgtttggt
     5641 gtctgtcatt ggttgtcctt tggcagcttt ggaagtggtt gccagtgggt tactgcaatc
     5701 acatcgtcat caagcccata ttgatttaca ctaagaaaat accactcgtt atcggcaagt
     5761 tctcttgaaa ctattaaaat taactgcatt cctgaaatat cgcataaccc tagcacatcc
     5821 gtttcaatag gtggtaattt gtcctcaatg gatatccagc cattattttc tacaagtgtt
     5881 ggtggttcaa taacttgttc aacttcccca aataactctg attctctttc ttcgtcggtt
     5941 agtgggcgga tatctgattt tgctctacct aaaacaacac cataaactgc atagggtaaa
     6001 tcataggctt catagctttc gtgatcatcc atgtcacccg caaactcata ggcttcagta
     6061 gcaccattta aacagctttg ttttgcttgc tctaaggttt cgtgcagatt taggatatgt
     6121 atatcatttg atacatccac cgaaaaaaat ttgtcttcat tcattttctt tctccaaaat
     6181 accaatagtt gagagaataa taaccgcttg tattcaacaa gcggtcaatt ttcattaaaa
     6241 acttacaagt aaacttataa gctaacttac aagctagatc tcaatcgtac cgatattgac
     6301 cttgattgat gtttcgctaa gtgcatcgga tagtttttcc gcaaactcag tcgcaatcgc
     6361 ctcttcaatt tgctcagctt gaactaagcg ggcggttaat accggctcac cgctaccggt
     6421 tagcacctgt acacgcagag taaatgatcg agtgtctaag ccgttatatg ggttgcaagt
     6481 aaatacaagg tgagttggta gttgcagtgt agatttagct tccaccgatt ccattgctga
     6541 ttttttggct gcgaaatcgc tgagttcatg ttcttcgttt cgggcgtagt caatcgtcat
     6601 tttacgcacc gcttggacgg catttttaat actgatttca tcttcgtggt ggtaagcggt
     6661 caagtagccg ccccaatctt cgaggaaatc agaaaaatca cgctgtgagc ggcgtgagcc
     6721 attaatttcg catagagctt tgtaggctgc ggttttcttc atcaccaaat tagcacgatg
     6781 tttagcgtga ccggcttgct ctcggttgcc catatcgaat acaatttccg cactcatttt
     6841 ctcttggtca ataaagcatt gtgcgttttc ttgggcattg gcggtagcat attcaatcag
     6901 gctaccaaaa cgttgcgttg aaaacgctgc acggaattga ttacggtgag cttggaaagg
     6961 ctctaaattt tgtaatttaa tgccttcagg taacattact gtgccaaaat cagtttgaac
     7021 ctctttagtc gcagctacag caagagtaga gatttgttga atagttgatt tatccatttt
     7081 gagttccttt ttgtttaagt aaaaataaag ccacgcatta acgtggcttg gattgaaaat
     7141 aattgattat gcagcagctt tagagagagc ctttaaaccg ctagggcttt cttttactgc
     7201 cgtttctgtt ttttcaggtg tagcagataa tgccccacct ttatgaacat acattggtgt
     7261 ggcggtagta tcttcttcgg ctgacttacc acgtttggtc ggtttgacat agctcaattt
     7321 gtgctgaatt tgaacagatg ggctgtcgct atccattttg tctaagctaa attcaaccgt
     7381 aactttccct ttcttatcgt gggttagtac gcctaacgcc acttctgaaa gagctgttgc
     7441 cagcttgtct ttgaaaatac cgcaatctaa ttcatctaag aattcaggta catttgtttt
     7501 tgccattttg ggttctccta attagggttt gaataaaatt attagaacgg aatatcatca
     7561 tcaaaatctc gtgattctga atccgtcatt acgtggttat atggattggt tggcggtggg
     7621 tttcctgccg gttcggttgc ccaattatta ttgctgtttc cgctgcttga gctaccaagc
     7681 atttgtagct gatcagcgat gatttcagtc gtccagcggt ctgtgccgtc ttgggcttgc
     7741 catttgcgag tacgtagttt gccctcaaca tagacttgtg agcctttttt caggtatttg
     7801 ccaataatgt cagctaaatt ccgaaaggcg ataatgctat gccactctgt ggcttgtttt
     7861 ttatcgcctg atactttatc tgtccattcg gtcgaagttg ctacgctgat tttggcggtt
     7921 gtatcgccgt ttggcattgt tcgcaattcc gggtcgttac ctaggcgacc gacaataatc
     7981 actttgttaa ttcctgccat tgttattcct tataaagttt aattggttta tctctgccaa
     8041 cgttgaaccg ctcaattttg cggttaatca acataattgc gttttggcgg ctgtattgtg
     8101 gataggttgt gtgctgtaat ttgccgtcat aaaaaaactt ggcgataaat agtttatcta
     8161 cctcaacaat ctcaacaccg gcatcaattt ttcgtgccat atttccctcc gcagtattga
     8221 ttgccattac tctcaacttg ccaaacgcct tgatttcgtt cgcatttgta acgttgcatt
     8281 tgtatgtggt cgtcataatc cattcgcccg acaatgccta atagcaaaat tagcaaggca
     8341 aatggggcaa gttgtttcat tttgttcata ggattgtcct ttagggttaa aaaaagccca
     8401 cgttttacgg tgggcaaatg gagcgacttg cgtctagttg tgggtattcc ggaacacact
     8461 tggtgaaata tgctttggaa taagtgcctt tctttatact tgtaaggctc aagtccctta
     8521 ttgcctctca caactctaag gaatataatc aaggctctca caactcaaat aaggattaag
     8581 ttatgaaaaa taatatttta attacttatg atttaaataa atcagggcaa aattacgcag
     8641 cactcattga aaaaattaaa actcttggag cttgggctaa agtacagcag tcagcttggt
     8701 atcttcatac ttcatattcc tcaaaagagg ttttagaaat gctaagcacc gtaactgatt
     8761 acaatgactc tctttttgtt gctcaattat ccgaggctaa ttggagagga ttatccaatg
     8821 aggttagtaa atttattcaa gaacaatggt ggaaatagac ctattcgcaa cttccattac
     8881 ctgtatttat tccgctttta ttgattgttt caattgcaga acaacatcgt gatgcaagca
     8941 tttctgggtc caagcaacca ttttctacag ctttaagtac agcctgtttg atatggtatt
     9001 tatcctcatc agacaggcca ttctctacaa cctttaattc caaatcttta gggcttccta
     9061 gttgttcatt taaaataaca actttcgcaa tttcaaaatt tttgtttttc ataattacct
     9121 cgtgtttaaa ttattccaaa acccactcat aataggcttt ggaataaatg cgtgtctgcc
     9181 cacgccgtcg cccgccctgc atttcgcctg ttcagtcggg tgctgcttgt atccgtactt
     9241 actcaaactt gagataagcg tgattgctta ggactgatac cagtgttgcc ttttcagctc
     9301 tccacacctt gcgagtgtgt tgcctaaacc ttgccacctc tgtttggcaa gtcgttcaaa
     9361 tttgtatgtc agggtctaac caaccttgcc gagtgataaa gcattacttt acaactgctc
     9421 gaggttgtta atatcgtccg cacaagcact aaattgttaa agaacatccc acagtctctg
     9481 cttatctctc ggctattgcc tgctatgtgg gctaggtcta gaacctttat tcaagccctc
     9541 agtaaagggc tttgataaaa attcttagtg aatgtaggct tgccaaagtt gttgagcttg
     9601 ttctgcgttc attttgtcgc tttggttcca gtcgattaac ttgccatttc tgaacattgc
     9661 tctttcaatc acaaactctt gcgtggtttc attttcaaag acctcaaaca cattgatgaa
     9721 atcactagtg cgtttttctt tacgtccgca taaaatgt
//
LOCUS       DANLUQ010000093         9731 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.093, whole genome shotgun sequence.
ACCESSION   DANLUQ010000093 DANLUQ010000000
VERSION     DANLUQ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9731
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.093"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(87..1370)
                     /locus_tag="PXB27_001718"
     CDS             complement(87..1370)
                     /locus_tag="PXB27_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5346484.1"
                     /translation="MTACHEPKRSIWQQIFTKNMLLCVYTGFCSGLPLFVLIQLMPAW
                     LTSANLDIKTIAAFTLTSLPYTWKFLWAALLDRYFPPFLGRRKSWIFISQIGLLVILA
                     SFGLFNPVQDISIIVTLSVLLAFLSATQDIVVDAFRREILSDNELGLGNSIHVNAYRI
                     AGLIPGGLSLFLADHYAWDTVFLITAAFLVPCLFVTLIIAAEPNHKPIDRSKPFYMAF
                     VDPFKEFFTRKGVAGAIGLILFIFLYKLGDSLATTLQTKFILDMGFTKSHIAGIVKIT
                     SLWCSIGGGIIGGVAMLKLGVNRALWLFGFVQLITILGFAYLASFEHFPTESIGAAEL
                     WKLGLVMAGEYLGVGLGTAAFVAFMARETNPAYTAMQLAIFTSLAALPSKMLGAYTGH
                     LVEMMGYYQFFWLCFFIGIPGMLMLFKVAPWGQAE"
     gene            1536..5252
                     /gene="metH"
                     /locus_tag="PXB27_001719"
     CDS             1536..5252
                     /gene="metH"
                     /locus_tag="PXB27_001719"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDL5346485.1"
                     /translation="MNTQHRTELLQNALTERILILDGAMGTMIQKYKLTESDFRGERF
                     KNSTIDLKGNNDLLTLTQPLLISAIHEKYLAAGADIIETNTFSATTIAQADYELESIA
                     YELNLVGAKLARLAADKFSIPEKPRFVAGILGPTNRTCSISPDVNDPGFRNITFMQLV
                     EAYAEATRGLIEGGADLIMIETIFDTLNAKAAVFAIETVFDELGVKLPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAKPLTFGLNCALGPKELRPYVEMMSKISETFVSVHPNAG
                     LPNAFGGYDLGAEEMATYIKEWAELGWLNIVGGCCGTTPEHIQAFAEATKGVAPRKLP
                     EIPTAMRLSGLEPLTIDDNSLFVNVGERNNVTGSAKFKKLIKEEKFAEAIEIAISQVE
                     NGAQVIDVNMDEALLDSKKCMTRFLNILATEPEAAKVPIMIDSSKWEVIEAGLQTVQG
                     KPIVNSISLKEGEEKFIHQAKLCRRYGAAVVVMAFDEVGQADTEERKVEICTRAYRIL
                     VDQLGFPPEDIIFDPNIFAIATGIEEHNNYGVDFIQACERIKRDLPHAKISGGVSNVS
                     FSFRGNNVVREAIHAVFLYYAIKAGMDMGIVNAGQLAIYDDLDPELREAIEDAVLNHR
                     HDTTDRLLEISEKYRGVKVESADESAAEWRNLPVAERLKHALVKGITTYIIEDTEEAR
                     QQFASPLEVIEGPLMAGMDVVGDLFGDGKMFLPQVVKSARVMKQSVAYLEPYINATKQ
                     KGSSNGKMVIATVKGDVHDIGKNIVSVVLQCNNFEVIDLGVMVPADVIIETAIREKAD
                     LIGLSGLITPSLDEMEYFLGEMNRLKLDIPVLIGGATTSKEHTAIKLYPKYNYPVVYT
                     ANASRCVTVCATLMNPEAKAEFWEKTKAEYEEIQRKFAVRKPLRNGLSIEEARANRFD
                     GFSGEWADYVPPTPKQTGIIEYKNVPIATLRKFIDWSPFFMLWGLMGGYPDAFDYPEG
                     GEEARRVYNDAQRVLDEFEQNGKLNPSGIMGIFPAVREGDDIAILNEDRTACIGKSYH
                     LRQQSERGKNSKSPYNLCLSDFIADRESGQQDWFGMFAVCAGIEEHYLVESYKAAGDD
                     YNAILLQAVGDRLAEAMAEYLHFELRTKVWGYTVEDEDNGRLIREEYIGIRPAPGYPS
                     CPEHTEKQIIWDLLEVEQRIGMKLTESYAMWPAASVCGWYFTHPTSNYFTLGKIDEDQ
                     VLDYAKRKGWDELTAKKWLSVAMKQDVKSQAENI"
     gene            5360..7363
                     /locus_tag="PXB27_001720"
     CDS             5360..7363
                     /locus_tag="PXB27_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HDL5346486.1"
                     /translation="MIEKQNYHLVQLLKDSNYQLTQFSPAYIKKLEESIFLKSGKNGD
                     IPHIQCLVRKKDIKLTPEEAVRQLYLMNLHYEYDYPFERMELEYSVVFGREKKRADIV
                     IFDRDNPNAVYIMVELKKPKLKDGKEQLKSYCNATGAPIGVWTNGESISYYNRRDPNY
                     FKDIPDIPNAQQKLSDLLQERWTIQDLIDNDKIAKQRKSLKDLVIEMEDEVLANAGVD
                     VFEELFQLIFTKLYDEMESTRNKSRYLEFRNYGDTESELKAKIQSLFDRAKNKWEGVF
                     AESAKINLSPSHLAICVSSLEEVKLFNSNLDVVDEAFEYLINKSSKGEKGQYFTPRYV
                     IDMCVKMLDPKPEETVIDTAAGSCGFPVHSIFHVWEKQLKARGLERSHLFTAEEKLPE
                     QTDYVKEKVFAIDFDEKAVRVARTLNLIAGDGQTNVLHLNTLDYERWEDFTKEEEWND
                     VYGEGWKKLRKLRKTKNENRDFQFDVLMANPPFAGDIKETRILARYELGKNSKGKQQS
                     KVGRDILFIERNLDFLKDGGRMAIVLPQGRFNNSSDKYIRDFIAERCRILAVVGLHGN
                     VFKPHTGTKTSVLFVQKWHDELCPKVEDYPIFFATMQKSSKDNSGDKIYVKNADGTLK
                     LDNHQHLIVEHDLFNHDNSTQDGIAEAFIEFAKKEKLSFVGKD"
     gene            7462..8820
                     /locus_tag="PXB27_001721"
     CDS             7462..8820
                     /locus_tag="PXB27_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL5346487.1"
                     /translation="MDSTFFLKKYLNHPSFNFPLSLNEVSLIKSGTTPTDRDDNLKEG
                     VVLLKTNDIRNNLLNKYSSVDYFISEDINEKMISSQLKEGDVLVNIVGATLEVVGRVA
                     YVSSTFPKANITQAMSFVRLKSKYNKELLPTYLFAFLQSSYGKIQINRNARPTGQYNL
                     NNEELGAIKVPLIDLETQKQVDKIIKQSNDFVQKSTQSYQEAETLLLENLGLRAFQAD
                     SNPVNVKSLKESFLQTGRLDAEYYQTKYEQYWNLIQSQDYVFIRDEYLHITQKPDWTK
                     PMYQYIEIGDVNIGDGSYQTNWIETQELPANAKTQAQTGDILISTVRPYRGAVTIIGE
                     NDQDLVVSGAFTVLRRKENSVFNNEVLKVLLRSELYKDWLLQFNVGTSYPVIKDNDIL
                     NLPIPKISGEIQEKIAEYIRQSNDLRQQAQNLLAQAKNNVEFEIENNSFNINELEVRG
                     GG"
     gene            8844..9578
                     /locus_tag="PXB27_001722"
     CDS             8844..9578
                     /locus_tag="PXB27_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL5346488.1"
                     /translation="MARSSYFARLAEWTLLEHLLVKQPENISVCSFGQSFGTSGRLDA
                     EYYQPKFASLFAQLANFKTMKLGEIVNLQKSVEPGSEAYQTEGIPFVRVSDLSKFGIS
                     QTDKYLHPKDFGNVVRPKKDSILLTKDGTVGIAYRVPQDLNVITSGAIVHLELKTDEV
                     LPDYLALALNSPAVQLQAERDAGGSIIQHWKPSEILDVVIPVLPKNIQQTISDKVQQS
                     FALRVESEVLLEKAKILVEQEIENMR"
BASE COUNT         3128 a         1904 c         2145 g         2554 t
ORIGIN      
        1 taaaaaataa ccgactaaat tactaggtaa ttcaatcggt tataagtggt cggatattta
       61 ataaaaaatc ttatttttat gaatagctat tcagcttgtc cccaaggagc aaccttaaac
      121 aacattagca ttcccggaat accaataaag aaacagagcc agaagaattg gtaatagccc
      181 atcatttcca ctaaatgccc tgtgtatgcc cctaacattt tgcttggtag ggcagcaagg
      241 ctggtgaaaa tggcgagctg cattgcagtg taagcgggat tagtttcacg agccataaag
      301 gcaacgaaag ccgctgtgcc taaacccacg cccagatatt cacctgccat cactaagcct
      361 aatttccaaa gctcggcagc tccaatgctt tcggtaggga aatgctcaaa actggcaagg
      421 taagcgaagc ctaaaatagt aattaattgt acaaatccga acagccataa ggctcggtta
      481 acgcccaatt tcaacattgc cacgccacca ataataccgc cgccaatgct gcaccaaagt
      541 gaagttattt tcacaatgcc ggcaatgtgc gatttagtaa aacccatatc caaaataaat
      601 ttggtttgta gcgtagttgc aagggaatcg cctaatttat ataggaaaat aaatagaatc
      661 agcccaatcg ctcctgccac gcctttacgg gtgaaaaact ctttgaacgg atcgacaaac
      721 gccatataaa acggcttaga acggtcgatt ggcttatgat tcggctcggc agcgattatt
      781 agcgtaacga ataagcaagg cactaaaaaa gcagcggtaa tcagaaatac ggtatcccac
      841 gcatagtgat cggctaaaaa taatgaaagc ccacccggaa tcagccctgc aatacgataa
      901 gcattaacgt ggatagagtt acctaacccg agttcgttat ccgataaaat ttcacgccga
      961 aaggcatcca ccacaatgtc ttgcgtggcg gaaagaaaag ctaatagcac agaaagagta
     1021 acaataattg aaatatcttg cactgggttg aacaagccaa agctggctaa aatcaccaat
     1081 agcccgattt gtgaaatgaa aatccagctt ttgcgtctgc ctaaaaaagg tggaaagtag
     1141 cgatctaaca atgcagccca caggaatttc cacgtgtagg gcaaagaggt cagggtgaag
     1201 gcggcaatag ttttaatatc taaattggca ctggttagcc aagccggcat tagctgaatt
     1261 aacacaaaca acggtaaacc ggaacaaaag ccggtgtaaa cacaaagcaa catattttta
     1321 gtaaaaatct gttgccagat agagcgtttg ggttcgtgac aagcggtcat atttttccta
     1381 cattttgcaa aataaaaagg ggaagaactg agccaatata gcataatttt aaaggcgact
     1441 ctaacccttt ccatttcgtc acagataagg tttgacagcc ttgcgacata tttctattat
     1501 tccttttaat caaactaaaa aactggaagc agagtatgaa cacgcagcat agaaccgaat
     1561 tacttcaaaa tgcccttacc gaacgcattc ttatcttaga cggtgcgatg ggaacgatga
     1621 tccaaaaata taaactaacc gaatctgatt tccgaggtga acgttttaaa aacagtacca
     1681 tcgatcttaa aggcaataac gacctactca cactcaccca gccactcctg atttctgcta
     1741 ttcacgagaa atatttagcc gccggtgcgg atattatcga aactaatacc tttagtgcga
     1801 cgaccattgc tcaagcagat tatgaacttg aaagtattgc ttatgagctg aatcttgtgg
     1861 gggcaaaact tgcccgtctt gccgccgata aattctccat ccctgaaaaa cctcgttttg
     1921 ttgccggtat tcttgggccg actaaccgta cttgttcgat ttcccctgat gttaatgacc
     1981 ccggtttccg taacattacc tttatgcaac tggtggaagc ctatgccgag gcaacccgtg
     2041 gtttgattga aggtggggcg gatctgatta tgatcgaaac cattttcgat accctgaatg
     2101 ccaaagcggc ggtgtttgca attgaaacgg tgtttgatga actgggcgtg aaattgccga
     2161 taatgatctc cggcaccatt accgatgcct cagggcgaac gctgtcaggg cagaccacgg
     2221 aggcgtttta taactcgctc cgccacgcca aaccgctgac gttcggcttg aactgcgcgc
     2281 tcgggccgaa ggaactccgc ccttatgtag agatgatgtc gaaaatcagt gagacttttg
     2341 tttcggttca cccgaatgcc ggcttgccaa acgcttttgg cgggtacgat ttaggggcgg
     2401 aagagatggc gacttacatc aaagagtggg cagaactcgg ctggctgaat attgtcggcg
     2461 gctgttgtgg caccacgccg gagcatatcc aagcctttgc cgaagcaacc aaaggcgttg
     2521 cgcctcgtaa attacctgaa attccaaccg ctatgcgttt gtcgggctta gaaccgctga
     2581 cgattgacga taattcgctg tttgtgaatg tgggcgaacg caataacgtc accggttcgg
     2641 caaaatttaa gaagctgatt aaagaagaaa aatttgcgga agcgattgaa attgccatca
     2701 gccaagtgga aaacggggcg caggtgattg acgtgaatat ggacgaggcg ttgctcgatt
     2761 ccaaaaaatg tatgacccgt ttcctcaaca ttcttgctac cgaacctgag gcggcaaaag
     2821 tgccgattat gattgactcc tcgaaatggg aggtgatcga ggcgggcttg caaacggtgc
     2881 agggcaagcc gatagtgaac tccatttcac tcaaagaggg cgaagagaaa tttattcacc
     2941 aagccaaact ctgccgccgc tatggggctg cggtggtggt gatggcattt gacgaagtcg
     3001 ggcaagcgga taccgaagaa cgcaaggtag aaatttgtac ccgagcttat cggattttgg
     3061 ttgatcagct cggcttccca ccggaagaca ttattttcga cccgaacatt tttgcgattg
     3121 ccaccggtat tgaagaacac aataactacg gcgtggattt tatccaagcc tgtgagcgga
     3181 ttaaacgaga cttgccacac gccaaaattt ccggcggggt gtcgaatgtc tctttctctt
     3241 tccgaggcaa caatgtggtg cgtgaggcta ttcacgccgt gttcctctac tatgcgatca
     3301 aagccggaat ggatatgggg atcgtgaacg cagggcagtt ggcgatttat gacgacctcg
     3361 accctgaact gcgagaggcg attgaagatg cggtgctgaa ccaccgtcat gacactaccg
     3421 accgcctgtt ggaaatttcc gaaaaatatc ggggcgtaaa agtagaaagt gccgatgaaa
     3481 gtgccgccga gtggcgaaat ttacctgtgg cagagcgttt aaaacacgcc ctcgtaaaag
     3541 gcataacgac ctacattatt gaagataccg aagaggctcg ccagcaattt gcctctccgc
     3601 tggaagtgat tgaagggccg ctcatggcgg gtatggacgt ggtgggcgac ctgttcggag
     3661 acggcaaaat gttcctgccg caagtggtga aatcggctcg ggtaatgaaa cagtccgttg
     3721 cctatttaga gccgtacatc aacgccacca agcagaaagg atcgagcaac ggcaaaatgg
     3781 tgatcgccac cgtgaaaggt gatgtacacg acatcggcaa aaacatcgtt agcgtggtgt
     3841 tgcagtgcaa taactttgaa gtgatagact taggcgtgat ggtgcctgcc gatgtaatta
     3901 tcgaaactgc tattcgggag aaagccgatc tcattgggtt aagcggacta attaccccat
     3961 cgctggacga aatggagtat tttttaggtg aaatgaaccg cttgaagctc gatattccgg
     4021 tgctgattgg cggcgcaacg acctcaaaag agcatacggc gatcaaactt tatccgaaat
     4081 ataactaccc cgtagtctat accgccaacg cttcacgttg cgtaaccgtc tgtgcgacct
     4141 tgatgaaccc tgaggcaaaa gccgaatttt gggaaaaaac caaagccgaa tacgaagaaa
     4201 ttcagcgtaa atttgccgtt cgcaaaccac ttcgcaacgg tttatctatt gaggaggctc
     4261 gagccaatcg ttttgacgga tttagtggcg aatgggcaga ctatgtgccg ccaacgccaa
     4321 aacagaccgg cattatcgag tacaaaaatg taccgattgc caccctacgc aaatttatcg
     4381 actggtcgcc gttctttatg ctctggggct taatgggcgg ttatcctgat gcctttgatt
     4441 atccggaagg cggcgaagag gcacgccgtg tctataacga tgcccaacgg gtgctggacg
     4501 aatttgagca aaacggcaag ctcaacccaa gtgggattat gggcattttc cctgccgtgc
     4561 gtgagggcga tgatattgca attttaaacg aagatcgcac cgcttgtatc ggcaaatcct
     4621 accacctccg ccaacaatcc gaacgaggca aaaacagcaa aagcccgtac aacctctgtt
     4681 taagcgattt tatcgccgac cgagaaagcg gacaacaaga ctggttcgga atgttcgccg
     4741 tttgtgccgg cattgaagaa cactatttag ttgaaagcta caaagcggca ggcgatgact
     4801 acaacgccat tttgctccaa gccgttggcg accgcttggc agaagcaatg gcggaatacc
     4861 tacactttga gctacgcacc aaagtctggg gctacaccgt ggaagacgaa gacaacggac
     4921 gcttaatccg tgaagaatac atcggcatcc gccctgcgcc cggctaccca agctgcccgg
     4981 aacacaccga gaaacagata atttgggact tgttagaggt ggaacaacgc atcggcatga
     5041 aactcaccga aagctatgcc atgtggccgg cagcaagtgt ctgcggctgg tacttcaccc
     5101 accctaccag caactacttc actctcggca agattgatga agatcaggtg ttggattacg
     5161 ccaaacgcaa aggctgggac gaactcacgg cgaagaaatg gttaagtgtg gcgatgaaac
     5221 aagatgtcaa gtcgcaggct gaaaacattt aatgtagtgg cgggttttaa cccgcctgtt
     5281 acaagcggtt ggttttgaga taaaatatgc aaaatttatc tctcttttat gcaaagaagt
     5341 tatcatcgag aacaaaacaa tgattgaaaa acaaaattat cacttagtac aacttttaaa
     5401 agacagtaat tatcaattaa ctcaattttc tcctgcgtat ataaaaaaat tagaggaatc
     5461 tattttcctc aaatcaggga aaaatggaga tattcctcat attcaatgtc ttgttcgtaa
     5521 aaaggatatt aagctcacac ctgaagaagc agtacgccaa ctctatttaa tgaatttgca
     5581 ttatgaatat gattatccat ttgaacgaat ggaattagaa tattctgttg tattcgggcg
     5641 tgagaagaaa cgagcggata ttgtcatctt tgatagagat aatcctaacg ctgtctatat
     5701 tatggtggag ctgaaaaagc cgaaattaaa agatggtaaa gaacaattaa aatcttattg
     5761 taatgcgact ggagcaccta ttggtgtatg gacaaatggc gaaagtattt cttactataa
     5821 tcgccgtgat cctaattatt ttaaagatat tcccgatatt ccgaatgctc aacaaaaact
     5881 ttctgattta ttacaagaac gttggactat tcaagatctg atagataatg acaaaattgc
     5941 caaacaacgt aaatcattga aagatcttgt tattgaaatg gaagatgagg tactggcgaa
     6001 tgctggagtt gatgtttttg aagagttatt tcaacttatt tttactaaac tctatgatga
     6061 aatggaaagt acacgcaata aaagtcgtta tttagagttt cgtaactatg gtgataccga
     6121 aagtgaatta aaggcaaaaa ttcaatctct atttgatcgt gcaaaaaata aatgggaagg
     6181 cgtatttgct gaaagtgcca aaatcaacct ttccccttcg catttggcga tttgtgtatc
     6241 ttcattggaa gaagtaaagc tgtttaattc taaccttgat gtagtggatg aagcatttga
     6301 gtacttaatt aataaatcca gtaaaggaga gaaaggacaa tactttacgc ctcgttacgt
     6361 cattgatatg tgcgtaaaaa tgctcgatcc aaaacctgaa gaaacagtca ttgataccgc
     6421 agcaggaagc tgtggttttc cagttcattc tatttttcac gtttgggaaa aacaacttaa
     6481 agcgagagga ttagaacgct cacacttatt tactgctgaa gaaaaattgc ctgaacaaac
     6541 agattatgtg aaagaaaaag tctttgcgat tgatttcgat gaaaaggcag tacgtgttgc
     6601 tcgtacccta aacttaatcg caggagatgg gcaaaccaac gtactacatt taaatacctt
     6661 agattatgaa cgttgggaag attttactaa ggaagaagaa tggaatgatg tatatggaga
     6721 aggctggaaa aaattaagaa aattacgtaa aactaaaaat gaaaatcgag atttccaatt
     6781 tgatgtgcta atggcaaatc cgccttttgc cggtgatatt aaagaaactc gtatattagc
     6841 tcgctatgaa ttaggcaaaa atagcaaagg aaaacagcaa tccaaagtcg gacgagatat
     6901 tttatttatt gaacgcaatt tagatttctt aaaagacggt ggacgaatgg cgattgtgct
     6961 accacaaggg cgttttaaca attccagcga taaatatatt cgtgatttta ttgcagagcg
     7021 ttgccgcatt ttagctgttg tgggcttaca cggaaatgtg tttaaaccac ataccggcac
     7081 aaaaacttcc gtactatttg tgcaaaaatg gcacgatgaa ctttgcccga aagtagagga
     7141 ttacccgatt ttctttgcca caatgcagaa atccagcaaa gataattcag gcgataaaat
     7201 ctatgttaaa aacgctgatg gcacactcaa attagacaac catcaacacc taattgtgga
     7261 gcacgatcta tttaatcacg ataatagtac ccaagacggc attgcagaag cctttattga
     7321 atttgctaaa aaagaaaagc tctcttttgt gggaaaggat taagcccatt tgaccctgct
     7381 aaatatcagg cattgttaga tgggctagag gtgagcgagg tttatttgag taaggttcgt
     7441 tctgcaagtt attcggaacg aatggatagt actttctttt tgaaaaaata cttaaaccat
     7501 ccgagtttta attttccatt atcgctgaat gaggtttctc tgattaaaag tggtacaaca
     7561 ccaacagatc gagatgataa cttaaaagag ggggttgtct tattaaaaac caatgatatt
     7621 aggaataatc tattaaataa gtactcttcg gtagattatt ttattagcga agatatcaat
     7681 gagaaaatga tttcatcaca actgaaagaa ggggatgttt tagtaaatat cgttggtgca
     7741 actttggagg ttgttggtcg ggtagcttat gttagtagca cttttccaaa agcaaatatt
     7801 acacaagcaa tgtcttttgt aagattaaaa tcaaaatata ataaagaatt attaccaacg
     7861 tatttatttg cttttttaca aagttcttat ggaaaaatcc aaattaaccg gaatgcacgc
     7921 ccaacaggac aatacaatct gaataatgag gagcttggtg cgattaaagt ccccttaatt
     7981 gatctagaaa cacaaaagca ggtagataaa atcattaagc aaagtaatga ttttgtacaa
     8041 aaatcaacac aaagttacca agaagctgaa actttattat tagaaaatct cggtttgaga
     8101 gcttttcaag ctgatagcaa tcctgtaaat gttaaatcct taaaagagag tttcttacaa
     8161 acaggacgat tagatgcaga gtattatcaa actaaatatg agcaatactg gaatttaatt
     8221 caatcgcaag attatgtttt tattcgtgat gaatatttac atatcaccca aaaacctgat
     8281 tggacaaaac cgatgtatca atatattgaa attggtgatg ttaatattgg tgatggttca
     8341 tatcaaacga attggataga gacacaagaa ttacctgcta atgctaaaac tcaagcccaa
     8401 acaggtgata ttttaatttc aaccgttcgc ccttatcgtg gtgcagtaac aattatcggt
     8461 gaaaatgatc aagatttagt tgttagtggt gcgtttacag tattacgcag aaaggaaaac
     8521 tctgttttta ataatgaagt tttaaaggtg ctattacggt cagaactata taaagattgg
     8581 ttactacaat ttaatgtagg tacgagttat cctgtaatta aagataacga tattttgaat
     8641 ttaccaattc ctaaaataag tggagaaatc caagaaaaaa ttgctgaata tatccgccaa
     8701 agcaacgatt taaggcagca agcacaaaat ttactcgctc aagctaaaaa taatgtagag
     8761 tttgaaattg aaaataattc atttaatatc aatgagttag aagtgcgggg gggggggtag
     8821 atcctgctaa gccttatggt ttattggcga gatcttctta ttttgcacgt ttagcagaat
     8881 ggactttatt agaacattta cttgtcaaac agcctgaaaa tatttcggta tgttcattcg
     8941 gtcaatcatt cggtacaagc ggtcgtttag atgcagaata ttaccaacct aaatttgcaa
     9001 gcttatttgc acaattagcc aatttcaaaa cgatgaaatt aggtgagatt gttaatttac
     9061 aaaaatcggt agaaccgggc tcggaagctt atcaaacaga gggaataccc tttgtgcgtg
     9121 tatcggattt atctaaattc ggcatcagcc aaacagataa atacttacat ccgaaagatt
     9181 ttggcaatgt tgtaagacct aaaaaagaca gtatattact gactaaagac ggcacagtcg
     9241 gcattgctta ccgtgttccg caagatttga atgtgattac atcaggggca attgtgcatc
     9301 tagagcttaa aactgacgaa gttttacccg attatttggc gttagcattg aattctcctg
     9361 ctgtacaatt acaagcggaa agagatgcgg gaggctctat tatccaacat tggaaaccgt
     9421 ctgaaatttt agatgtagtg attccggtat tacctaaaaa tatccagcaa accattagcg
     9481 acaaagtaca acaaagtttt gcattgagag tagaaagtga agttttactg gaaaaagcta
     9541 aaatacttgt agaacaagaa atagaaaata tgaggtaata atgcctaatc ataatcaaga
     9601 tttgaaaaaa gagtggttta ataaagcaag gatagattac cactctcctt ttttaatgtt
     9661 atggctcagt tgtaatagct ggtataactt ccattattcc ttaggtaagg atagaacaca
     9721 tattgataga a
//
LOCUS       DANLUQ010000094         9519 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.094, whole genome shotgun sequence.
ACCESSION   DANLUQ010000094 DANLUQ010000000
VERSION     DANLUQ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9519
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.094"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..358
                     /locus_tag="PXB27_001723"
     CDS             <1..358
                     /locus_tag="PXB27_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346489.1"
                     /translation="AELKYSGSNIPSEYSKFNLENVLIDFPNKTNSVAYQNLVIHNAK
                     TRAGKLKTQYANAHDLGNLVLVEDIQKIFSGLLEIIYQVRCHLVHGSLSPTPENHEVV
                     KYCYLILWDCLKGFCD"
     gene            complement(626..1363)
                     /locus_tag="PXB27_001724"
     CDS             complement(626..1363)
                     /locus_tag="PXB27_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5346490.1"
                     /translation="MIKIENLTLAHGIQHLSCYLPKGKLIGIMGANGAGKSSLLKAIA
                     GILRPTSGEIWLENCKLHALSAVERSRQFAYLAQQLNVAWQLSVYDVIALGLPAPLPP
                     EQERQKVRSVAEQLAISHLLDNTYQQLSGGEQPRVQLARCLMKQAPILLADEPIAALD
                     PYYQIDIMQQLKALTPTQTCVVVIHHLPLAYRFCDEIILLKNGNLLVSGNTQAVLNAE
                     NIATAFGVKAEMDWEKREMWGIEKLEV"
     gene            complement(1360..2328)
                     /locus_tag="PXB27_001725"
     CDS             complement(1360..2328)
                     /locus_tag="PXB27_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL5346491.1"
                     /translation="MKKLNIVLGSLLLLIVSNAAFYQLSDFALLKNAEGVLSDLRWLV
                     LGEIRLPRIGLAVLTGASLALAGNAMQGVFKNPLASPGLLGSSSGATAVSVFLLYYFS
                     APMGWLLFGGVAGALGAFLLVWLISFRHGTTMMILSGVAVNVLLGAAVTLLLSNAESP
                     WALAELYRWLQGSLVWATAEAVCWALPLILLGIVCLYRERRYLDLLTFGEETAATMGI
                     NLQRSFFTVSIGVALLVGATIPQTGTIGFIGLIAPHLARIWLKKPPSQLYLTSALIGA
                     LLLLIADLAVQYVPFFARIYIGTLTAILGVPFLIWILFTQQWRLAQ"
     gene            2509..2844
                     /locus_tag="PXB27_001726"
     CDS             2509..2844
                     /locus_tag="PXB27_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5346492.1"
                     /translation="MYHIERTEIFDQWLENLKDPLAVVTIARRIERAESGNFGDTKYI
                     AEGISEMRLNVSKGYRLYYAQKGSIIYILLNGGHKGTQQADIDKAKMLWAEIKQHMAE
                     QEKKNERII"
     gene            2828..3151
                     /locus_tag="PXB27_001727"
     CDS             2828..3151
                     /locus_tag="PXB27_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative addiction module antidote protein"
                     /protein_id="HDL5346493.1"
                     /translation="MNELYDAPIPQATREKFGIQPFDAAEYLANDELIALYLAETLKD
                     GTDEEFIQALNTVARAKGMNELAKKAGIGRESLYQSLSSSKPRFETIRKIISALNLEL
                     SVVKA"
     gene            complement(3141..5027)
                     /locus_tag="PXB27_001728"
     CDS             complement(3141..5027)
                     /locus_tag="PXB27_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HDL5346494.1"
                     /translation="MTQLDSVTVYSAYATPINQDKTASSVTVLTEKDFAARNATYVSD
                     VLKTVPGVAIGQQGGRGTLTSLFLRGAESRHTAVVIDGVKVNPINIGNFDFGGLPISN
                     IERIEVLRGEQSALWGSSAMGGVVYITTKSGLYKEKPFNAEVDLGLGSNNTRDASATL
                     SGFHNGFYYALHGDSHRTKGISALSKNHFSYTTETGSEVKTGGASERDGFHRDNGSLR
                     LGYDLGNKGVEVLAAQSSQTVHIDGYNSDVSGEYSRTRNQTFKLGGYWGNEQELLKHQ
                     ANISQFNSKATHFGSNARYSNEKQLNANYQLDVNFDREGEVTQAVSLLTDYAKTRYTS
                     DKYLREKTLSEKSAALEYRLFTEQDHSFSISGRYTDNSQFKNSITGRISGAYRLSPNL
                     CSDRLLLELAEPQQIRAMSPYSQKPLMMLDKLNTDKPTVEPELTALLPYADSTILLNE
                     TFYPQLTARLKQLGFKLVALNDSPQTPEQLFTLILQLGELTQNQAKAEKLVERLRLQK
                     IPLKQPLAETLILSETGMIEPHFPQYQTLLDLLGLSPLKSDLTPQNFSLEKLLLAQPK
                     QLLFLTDNQSYNNQAELLKHPALQKIWQKMSQNPPLVLPMKYTYCFDHGVWQGIQLMH
                     KLTP"
     gene            5057..5857
                     /gene="metF"
                     /locus_tag="PXB27_001729"
     CDS             5057..5857
                     /gene="metF"
                     /locus_tag="PXB27_001729"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HDL5346495.1"
                     /translation="MQQKWRMLWDSIDRLSPLKPKFVSVTYGANSGERDRTHSIVKSI
                     QEKTGIISAPHLTGIDATPEELKVIAKDYWDSGIRRIVALRGDIPKGYDKALFYASDL
                     VALLKSVADFDISVAAYPEVHPEAKSAQADLLNLKRKIDAGANHVITQFFFDVESYLR
                     FRDRCVSIGIDCEIVPGILPVTNFKQLQKMAAVTNVKIPTWMGKMYEGLDDDQTTRNL
                     VAASIAMDMVKVLSREGVNDFHFYTLNRSELTYAMCHTLGVRPDLTTA"
     gene            complement(5906..7066)
                     /locus_tag="PXB27_001730"
     CDS             complement(5906..7066)
                     /locus_tag="PXB27_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346496.1"
                     /translation="MKKSILLILSLFSASLFADVQLSPLPLPQIGKVSQFDLSEQGEL
                     VVINQQKQLWRVTQQAQKLADGVSTEIEPKAQYQRIALADQNGHFMLWTADKIYTSNI
                     PLAPKATMTALNFATIAVTKQNGQFKLARIETQGNQATITATAETEVLPDAHPLQIDF
                     DAETPNQGHIAVLAKPDDFTYQHGVLGDDVEAGEIQFLERHTLNPLAEKLTQQGRVFE
                     ANRWATFTENGKTKIVSVISGDGNGASTVVIGVKDGKLSIEAESEPLSTNRWQSPFIF
                     NRKLYAIQMPHLKGNLVEYSLKGNQLAERFITSGLSNHRYGDHETNLVATTETFALIP
                     QMGYQQVVVLDKTGKITELPTALPAGIKQTKADSNKAYLLLKNGEIWVAEEK"
     gene            complement(7126..8142)
                     /gene="galE"
                     /locus_tag="PXB27_001731"
     CDS             complement(7126..8142)
                     /gene="galE"
                     /locus_tag="PXB27_001731"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDL5346497.1"
                     /translation="MAILVTGGAGYIGSHTLVELLNENREIVVLDNLSNSSEVSLERV
                     KQITGKSVKFYQGDILDRDILRKIFAENQIESVIHFAGLKAVGESVREPLRYYQNNVT
                     GSIVLVEEMLKANVNTIVFSSSATVYGDPQIIPIVESCPVGGTTNPYGTSKYMVERIL
                     EDTVKAFPQLSAVVLRYFNPVGAHESGLIGEDPNGIPNNLMPFISQVAVGKLPQLSVF
                     GGDYNTHDGTGVRDYIHVVDLALGHLKALDKHQNDAGFHVYNLGTGTGYSVLDMVKAF
                     EAANGITIPYKVVDRRPGDIAVCYSAPQKALEQLGWETERGLEQMMKDTWNWQKNNPN
                     GYKG"
     gene            complement(8230..8391)
                     /locus_tag="PXB27_001732"
     CDS             complement(8230..8391)
                     /locus_tag="PXB27_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HDL5346498.1"
                     /translation="MNETLLNNLVCPVSNEKLEWDKENNRLINPKLNIAYPIKNGIPE
                     LLPEAGVAL"
     gene            complement(8369..9370)
                     /gene="lpxK"
                     /locus_tag="PXB27_001733"
     CDS             complement(8369..9370)
                     /gene="lpxK"
                     /locus_tag="PXB27_001733"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HDL5346499.1"
                     /translation="MKIWQTKSLITWLLSPLSLLFWLISQARKRLYSLNILQSYRSPL
                     PMLVVGNISVGGNGKTPVVVWLVEQLQQRGVKVGVISRGYGGKAKTFPQLVTRESSAE
                     MVGDEPVLIVQRTNVPLAISPNRQQSIELLLNQFEPDIIITDDGLQHYALQRDMEWVV
                     VDGVRRFGNGFVLPAGGLRELPSRLNSVQAVICNGGTPQANEHLMTLEPSDAINLVTN
                     QRKPLSEFTQPCVAIAGIGHPPRFFTMLEKLGIPLAKTQGFADHQAFDKAQLAQFADS
                     QTPLLMTEKDAVKCHDFAQPNWWYVPVSATFSEKSTACLLAPILQKLGKEDERNTTQ"
     gene            complement(9372..>9519)
                     /locus_tag="PXB27_001734"
     CDS             complement(9372..>9519)
                     /locus_tag="PXB27_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL5346500.1"
                     /translation="GNGVEEIQRLQLIISHKDELLAHKEEIIENQKRELALLRDLVET
                     LKNK"
BASE COUNT         2609 a         2071 c         2114 g         2725 t
ORIGIN      
        1 ggcagagtta aaatattcag gtagcaatat tccaagtgaa tattctaaat ttaacttgga
       61 aaatgtgctt attgattttc ctaacaagac aaattctgta gcatatcaga atctagttat
      121 tcacaacgca aaaacgcgtg caggaaaatt aaaaacacaa tatgcaaatg ctcacgattt
      181 aggcaatttg gttttagtag aggatatcca aaaaatattt tccgggttat tagaaataat
      241 ttatcaagtt cgttgccatt tggtacatgg tagcttaagt ccaacacctg aaaaccacga
      301 agttgttaaa tattgctatt tgattttgtg ggattgttta aaaggatttt gtgattagtt
      361 attgtcaata aagctacaag cggtcaattc tcccaaaact ttgcaaattt ccccgcaaaa
      421 ctgaccgctt gttctcctct atccattcac ctcaaccaac ttttctctct acccctactc
      481 aaaatcggtt aaacaatctg ttgaggtaaa tgttgtgtcg gaatgggatt taatcttaat
      541 gaaactgaaa aaatcttctg ccaagcaaat ttccctaaaa ttggaagttc atttccaatt
      601 tctcaaaaaa tcacccaaac cggaactaca cttccaattt ttcaatcccc cacatttccc
      661 ttttctccca atccatttca gctttcaccc caaaagccgt tgcaatattt tcggcattta
      721 agaccgcttg tgtgttgccg ctgacgagta aattgccgtt tttaagcaga atgatttcat
      781 cacaaaaacg gtaggcaagg ggcaaatgat gaatcaccac cacgcaggtt tgtgttggag
      841 ttagggcttt gagctgctgc ataatatcga tttggtagta ggggtcgagg gcggcaatcg
      901 gttcatcggc aagcaagatc ggggcttgtt tcatcaaaca acgggcgagt tgcactcggg
      961 gctgttcacc gccggagagc tgttggtaag tgttgtccag caggtgagaa atcgccaatt
     1021 gttcggcaac cgaacgcacc ttttgacgct cttgttcagg cggaagcggt gcgggcaagc
     1081 cgagtgcaat cacatcatac accgaaagtt gccacgccac gttgagctgt tgggcgaggt
     1141 aggcaaattg tcggctcctc tcaacggcgg aaagggcgtg cagtttgcaa ttttccagcc
     1201 aaatttcacc gcttgtcggg cgtagaatgc cggcaatcgc tttgagcaac gaggatttcc
     1261 ctgccccgtt tgcgcccata atgccgatca atttgccttt tggcagatag caagagaggt
     1321 gttgaatacc gtgggcaagg gtgagatttt caatcttgat cattgtgcca atctccattg
     1381 ctgggtgaat aaaatccaaa tcaaaaacgg tacgcccaaa attgccgtga gcgtgccgat
     1441 gtaaattcgg gcaaaaaacg gcacatattg caccgccaga tctgcaatca gcagtaacaa
     1501 cgccccgatt aaagcggagg taagataaag ctgggacggc ggttttttca gccaaatacg
     1561 ggcaagatga ggggctatca agccgataaa accgatggtg ccggtctgtg gaatcgttgc
     1621 tccgaccaac agcgccacgc caatgctaac ggtaaaaaaa ctgcgttgca ggttgatgcc
     1681 cattgttgct gccgtctctt cgccaaaggt gagcaggtcg agataacgcc gttcccgata
     1741 gaggcagaca atgccgagca agatcaacgg taacgcccaa catactgcct ctgcggttgc
     1801 ccagactaat gagccttgca accagcggta gagttccgcc aacgcccacg ggctttcggc
     1861 attggaaagc agtaaagtca ccgctgcccc gagcaacacg tttaccgcca cgccacttaa
     1921 aatcatcatt gttgtgccgt ggcgaaagct aatcagccac accagcaaaa atgcccccag
     1981 cgcccctgcc acaccgccaa acagtagcca gcccatcggg gctgaaaaat agtagagcag
     2041 aaatacgctc accgccgttg cccccgaact gctcccaagc aaaccggggc tggcaagcgg
     2101 atttttaaac acgccctgca tcgcattgcc ggcaagcgca aggctggcgc ccgtcagcac
     2161 cgccaagcca atacggggca gtcgaatttc acccagcacc agccagcgca gatcgcttag
     2221 cacgccttcc gcatttttaa gcaaggcaaa atcgctcagt tggtaaaagg ctgcgttgct
     2281 aacaatcagc aacaacaagc ttccaagcac aatgtttaat ttcttcattc ttctttctct
     2341 catcaaacgt gcaaattata ccatagcgcc aaagtcaagc tgaaaacgct atttttgcga
     2401 tcatcatcga aaaactagcg tttgccgaat gtaacccacg acaggctttg ctatattcct
     2461 tcaatcagcc taagttttgt atttaaaaca ttacagattg ggggtagaat gtaccatatt
     2521 gaacgcactg aaattttcga ccaatggctt gaaaatttaa aagatccact tgctgtggtg
     2581 accattgcaa gacgtattga acgtgccgaa agcggaaatt ttggcgatac caaatatatc
     2641 gcagaaggaa tttccgaaat gaggctaaac gtcagcaaag gttatcgcct ttactatgcg
     2701 caaaaaggta gcattattta tatactacta aatggcggac ataaaggcac gcaacaagct
     2761 gatattgata aagctaaaat gctatgggca gaaattaaac aacatatggc agaacaggag
     2821 aaaaagaatg aacgaattat atgacgcccc aattccacaa gctactcgtg aaaaatttgg
     2881 cattcaacct ttcgatgccg ctgaatacct cgccaatgac gagctaatcg ccctttattt
     2941 ggcggaaaca ttgaaagacg gcacggacga ggagtttatt caagcattga ataccgtggc
     3001 acgagcgaaa ggaatgaatg agttagcgaa aaaagcaggg atcgggcgtg aaagtttgta
     3061 tcaaagcctt tccagcagta agccccgttt tgagactatc cgcaaaatta ttagcgcatt
     3121 aaatttggag ttgtcggtcg ttaaggcgta agtttgtgca ttaattgtat cccctgccaa
     3181 accccgtggt cgaaacagta ggtgtatttc atcggtagga caagcggtgg attttggctc
     3241 attttttgcc atattttttg taatgctggg tgtttgagca gttcggcttg gttgttatag
     3301 ctttggttat cggtgaggaa caataactgt ttgggttgcg ctaacagcag tttttctagc
     3361 gagaaattct gtggcgtgag gtcgcttttt aatggtgaca gaccgagaag atccaacaac
     3421 gtttgatatt gcggaaaatg cggttcgatc atacccgttt cgcttagaat taaagtttca
     3481 gcaagcggtt gttttagcgg aattttttgc aaacgtagcc gttccaccag cttttccgct
     3541 ttggcttgat tttgggttag ctcgcccagt tgtaaaatca gtgtgaaaag ctgttcgggt
     3601 gtttgcggac tgtcgtttaa tgcgactaac ttaaagccaa gctgtttcag ccgtgcggtg
     3661 agttgcggat agaaggtttc attcagcaaa atggtgctgt cggcataggg caacagggcg
     3721 gtgagttcag gctcaacggt gggcttgtcc gtgttcagtt tatccaacat catcaacggc
     3781 ttttgggaat agggcgacat cgcccgaatt tgctgtggtt cggcaagttc cagcaacaga
     3841 cggtcgctac acaaatttgg cgataaacgg taagctcctg aaatacgccc agtgatagaa
     3901 tttttaaact gtgaattatc ggtgtaacgg ccactaatag aaaaactgtg atcttgttcg
     3961 gtaaataagc gatattctag tgccgcactt ttttcggata aggttttttc acgcaaatat
     4021 ttatcagagg tatagcgtgt ttttgcataa tctgtcagta aactgaccgc ttgtgtcact
     4081 tctccttcac ggtcaaagtt tacatctaat tgataattcg cattaagttg tttttcgttg
     4141 ctgtagcgag catttgaacc gaaatgtgtt gctttactat tgaattggct gatatttgct
     4201 tggtgtttca gcagctcttg ttcatttccc caataaccgc ctaatttaaa agtttgattg
     4261 cgagtacggg aatattcacc gctaacgtct gaattatagc catcaatatg aacggtttgg
     4321 gaagattgag cggcaagaac ttctacccct ttgttaccga ggtcataacc taaacgtaaa
     4381 gagccgttat cacgatgaaa gccatctcgt tcgcttgcac caccggtttt cacttctgag
     4441 cctgtttctg tggtatagct gaaatggttt ttactcagtg cggaaatgcc tttggtacga
     4501 tggctatcac cgtgtaaggc atagtagaaa ccgttgtgaa atccggaaag tgttgcgctg
     4561 gcatcacggg tattatttga gcctaaacca agatcgacct ccgcattgaa tggtttctct
     4621 ttgtataaac cgcttttagt ggtgatataa actacaccac ccatagcact gcttccccaa
     4681 agggcggatt gctcgccacg caacacttcg atacgttcga tattgctaat cggtaagcca
     4741 ccaaaatcga aatttccaat attaatcgga ttgactttta caccgtcaat cacaaccgca
     4801 gtatggcgag attctgcccc tcgtaaaaat aagctagtaa gagtaccacg cccaccttgt
     4861 tgcccaatcg ccacgcccgg cacagtcttt aacacatcac tcacataagt cgcattgcgt
     4921 gcggcaaaat ctttttcggt aagaaccgtc acgctggaag cggttttgtc ttggttgatt
     4981 ggggttgcat aggcggaata gactgttacg gaatcaagct gagtcatctc attttgtgca
     5041 aaagctgtac ttgctagtgc aacagaaatg gcgaatgctg tgggattcta ttgatcgcct
     5101 aagtccttta aaaccgaaat ttgtttcggt aacttatgga gcaaattctg gtgagcgaga
     5161 tcgtacacac agtattgtga aatcaatcca agaaaaaaca gggatcattt cagctccgca
     5221 cttaacaggc attgatgcaa caccggaaga attaaaggta attgccaaag attattggga
     5281 tagtggtatt cgccgaattg ttgctttgcg tggcgatata ccgaaaggtt atgataaagc
     5341 actattttac gcctcagatt tagtcgcttt attaaaatca gtagcagatt tcgatatttc
     5401 cgtggctgcc taccctgaag ttcaccctga agcaaaatca gcacaggcag atttacttaa
     5461 cttgaaacgt aaaatcgatg cgggtgcaaa ccacgttatt acgcaattct tttttgatgt
     5521 agagagttat ttacgttttc gtgatcgctg tgtttctatt gggattgatt gtgaaattgt
     5581 gccgggtatt ctgccggtga caaatttcaa gcagttgcaa aaaatggcag cagtgactaa
     5641 tgttaagatc ccgacttgga tgggtaaaat gtacgaaggg ttagatgatg atcaaaccac
     5701 cagaaactta gttgcagcaa gtattgcaat ggatatggtg aaagtgcttt ctcgtgaagg
     5761 ggtgaatgat ttccacttct ataccttaaa tcgtagtgaa ctcacttatg caatgtgcca
     5821 tactttaggc gtaagacctg atttaacaac tgcataatcg aacattaagg ctgtatagct
     5881 aaatgagcta tacctcctta tagttttact tctcttcagc cacccaaatc tcgccatttt
     5941 ttaataataa ataggctttg ttggaatcgg ctttggtttg tttaattcca gccggcaggg
     6001 cggttggcaa ttcagtgatc ttgcctgttt tatccaacac aacaacttgt tgatagccca
     6061 tttggggtat cagtgcgaaa gtctctgttg tcgccactaa attggtttcg tgatcaccat
     6121 agcggtgatt gcttaagccg gaggtaataa aacgttcagc taattggttt cctttcaggc
     6181 tgtattcaac caaattgcct tttagatgtg gcatttggat tgcatagagc ttgcggttaa
     6241 agataaacgg cgattgccaa cggttagtcg acagcggctc actttcggct tctattgata
     6301 attttccgtc tttaacgcca atcacaacag tgctggcccc attgccatcg ccagagataa
     6361 cagaaacgat cttggttttt ccattttctg tgaaagtcgc ccagcggttg gcttcaaata
     6421 ctcgcccttg ttgggtgagt ttttctgcta aggggtttag ggtatggcgt tctagaaatt
     6481 gaatttcgcc tgcctctaca tcatcgccta atacaccgtg ttgatatgta aaatcatcag
     6541 gctttgcaag tacagcaata tgcccttggt taggtgtttc ggcatcaaaa tcaatttgca
     6601 gagggtgagc atcaggcaac acctcggttt ctgcggtggc agtaatagtg gcttggtttc
     6661 cttgcgtttc aattctggct aacttaaatt gtccgttttg tttggtcacg gcaatggtgg
     6721 caaaattaag ggcggtcatt gtagctttcg gggcaagggg aatgttggag gtatagattt
     6781 tatcagccgt ccacagcata aaatgcccgt tttgatccgc taacgcaatt cgttggtatt
     6841 gagctttagg ttcaatctcg gtggaaactc catctgccag tttttgagct tgttgtgtga
     6901 ctctccataa ttgtttttgt tggttaatta ccactaattc gccttgttct gaaaggtcaa
     6961 attggcttac tttgccaatt tggggaagtg ggagtggaga aagctgaaca tcagcaaaca
     7021 aagaagcgga gaacaagctt aagattaaca gaatagattt tttcattagc gttacctaaa
     7081 aagaagattt aaatgataaa gggagatgtt atctcccttt acgctttagc ctttataacc
     7141 attcgggtta tttttttgcc aattccaagt gtctttcatc atttgttcta gcccacgctc
     7201 ggtttcccaa cccagttgct ctaacgcttt ttgtggagcg gagtagcaaa cggcaatatc
     7261 gcccgggcgg cgatctacca ctttgtatgg aatagtaatg ccatttgcgg cttcaaaggc
     7321 ttttaccata tctagcaccg aataacctgt tcctgtgcct aaattgtaaa cgtggaaacc
     7381 ggcatcattt tggtgcttgt ctaaggcttt taagtgacca agtgctaaat ctaccacgtg
     7441 gatataatca cgcacacctg tgccatcgtg agtattataa tcgccaccaa acaccgataa
     7501 ttgaggtaat ttgcccacag ccacttggct gataaacggc attaagttat ttgggatacc
     7561 gtttggatct tcaccgatta aaccgctttc gtgagcccct accgggttga agtaacgcaa
     7621 tactaccgca cttaattgtg ggaaggcttt aacggtatct tcaagaatgc gttcaaccat
     7681 atatttggac gtaccgtaag ggttggttgt gccaccgaca gggcaagatt ccacaatcgg
     7741 gataatctgc ggatcaccat aaacggtcgc cgatgaacta aacacaatgg tattcacatt
     7801 ggcttttagc atctcttcta ccaatacaat cgagccggtt acattgtttt gatagtaacg
     7861 tagcggttct ctgacgcttt cgcctacagc ttttaaaccg gcaaagtgaa ttaccgattc
     7921 aatctgattt tccgcaaaaa ttttgcgtaa aatatcacga tctaaaatat cgccttgata
     7981 gaattttacg ctcttgccgg tgatctgctt cacacgctca agtgatactt cagaggaatt
     8041 agaaagatta tctaacacca cgatttcacg attttcgttt aataattcaa ctaatgtatg
     8101 tgaaccgatg tagcctgcac cacctgtaac taaaattgcc attttatgtt cctctataaa
     8161 attaatacga accaaggtta ttgttgctat tttacccgat ttaatcaggc ttgctaatat
     8221 ttattcagtt tataatgcca ctccggcttc cggcagtaat tccggaatgc cgtttttaat
     8281 cgggtaagca atattgagct tagggttaat taatcggttg ttctctttgt cccattctaa
     8341 tttttcatta gagacagggc agacgaggtt attgagtagt gtttcgttca tcttcttttc
     8401 ctagtttttg tagaatcggg gctaacaaac aagcggtcga tttttctgaa aatgttgcag
     8461 aaaccggtac ataccaccaa tttggctgtg caaaatcgtg gcattttacc gcatctttct
     8521 cggtcattaa taacggcgtt tggctatcag caaattgagc cagctgagcc ttatcaaagg
     8581 cttgatgatc ggcaaagcct tgagttttgg caagcggaat gcctaatttt tctaacatcg
     8641 taaaaaagcg tggtgggtga ccgataccgg caatcgccac acagggttgg gtaaattcgc
     8701 ttaatggctt gcgttgattg gtgactaaat taattgcatc agacggttca agcgtcatta
     8761 aatgctcgtt agcttgcggt gtgccgccgt tgcaaataac cgcttgcacg gagtttaaac
     8821 ggctcggtaa ttcacgcaat ccgcccgccg gcagcacaaa accgttaccg aaacgcctta
     8881 cgccatcaac caccacccat tccatatctc gctgtagggc atagtgttgc aagccatcat
     8941 cggtgatgat gatgtccggc tcaaactgat ttaatagtaa ttcaatgctt tgttggcggt
     9001 ttggagaaat agccaaaggc acattggttc gctgaacaat cagaaccggt tcatcaccca
     9061 ccatttcagc agaactttct cgggtaacta attgcggaaa cgttttcgct ttaccgccat
     9121 agcctcgaga aatcacgcct acttttacgc cacgctgttg cagttgttcc accagccaaa
     9181 cgaccactgg cgttttgccg ttgcctccaa ccgagatatt accgactacc agcataggca
     9241 aaggcgaacg gtacgattgc aaaatattca gagaatataa ccgcttgcga gcttgactaa
     9301 tcagccaaaa cagcaacgaa agcggcgaaa gcagccaagt gataagggat tttgtttgcc
     9361 agattttcat attatttatt tttaagtgtt tcaactaaat ctcgtaataa ggctaattca
     9421 cgtttttggt tttcgataat ttcttcttta tgggctaata attcatcttt gtgggagata
     9481 ataagttgta aacgttgaat ttcttctact ccatttcct
//
LOCUS       DANLUQ010000095         9387 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.095, whole genome shotgun sequence.
ACCESSION   DANLUQ010000095 DANLUQ010000000
VERSION     DANLUQ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9387
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.095"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            10..501
                     /locus_tag="PXB27_001735"
     CDS             10..501
                     /locus_tag="PXB27_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL5346501.1"
                     /translation="MQTKPILDACCGSRMFHFDKQNPHVLFADNRQIETTMKDSGKVR
                     HLEIKPDVLHDFTNMPYENESFWHVVFDPPHLVQGGENSWLVKKYGKLPCNWREVLKK
                     GFDECMRVLKPNGTLIFKWNETQIPVSEILKVIDCQPIYGHKSGRLNQTHWLAFVKIE
                     QSL"
     gene            527..802
                     /locus_tag="PXB27_001736"
     CDS             527..802
                     /locus_tag="PXB27_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HDL5346502.1"
                     /translation="MEQPTEFLTKDELTDLTGYRQKKRQIEWLRERHYIIQDENKYKP
                     LVLYKDVYGHPRQQYPEAQSTGKWKSPAYLMGVKNGKTTQTTESLLT"
     gene            762..1817
                     /locus_tag="PXB27_001737"
     CDS             762..1817
                     /locus_tag="PXB27_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL5346503.1"
                     /translation="MGRPRKQQNRYLPENLRPKTVTRKSGKTVIYWLYRTHGKTEISL
                     GTNRNEAFIKAAQLNIERESQTARITFGLVAKRYRDEVIPQKKASTAQTDLCKIKNLL
                     LFFNDAPLEEITPQHIREYMIWRKDTPGSANNEFSMFSHIWQYAREWGYTNLPSPNIG
                     MKRYPIRKREIYVENNIFQMVYEVADQDMRDLMDLAYITGQRPVDLVNIRKEHIFDGY
                     LHIVQQKTQAKLRIEIVGKLADIIQDRLEKATGTHLFSTKKGEKLTARKLGKRFTYIR
                     EQAVKNNPEYANEIMAFQFRDLRAKSGTDKALSLGEEAARQQLGHTSVQMTKTYIRKA
                     PIVTPIKESVPSSKHQS"
     gene            complement(2069..2380)
                     /locus_tag="PXB27_001738"
     CDS             complement(2069..2380)
                     /locus_tag="PXB27_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL5346504.1"
                     /translation="MSETEKQAINAPNAVMNGYLTMHYPDWFKPDGIYFNDGAFESFE
                     SSHKLTKDGKIRLVPTAGHTLGHLAVVVDMGEHYILIGGDASYSEQDMLAGNIDGVCN
                     A"
     gene            complement(2387..2779)
                     /locus_tag="PXB27_001739"
     CDS             complement(2387..2779)
                     /locus_tag="PXB27_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL5346505.1"
                     /translation="MIKVHCLTTGWVQIKIHHQVARFFARPARVLDVLTDKKWSPKLP
                     IGCWLIEHDDGLILVDTGESSRANDKGYQPWWHPFMQFCERRGVKKHEEVGEILKANG
                     FDPLRIDTVVMTHMHGDHAGGLPNFPNS"
     gene            2911..3471
                     /locus_tag="PXB27_001740"
     CDS             2911..3471
                     /locus_tag="PXB27_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDL5346506.1"
                     /translation="MNDLRVIKTHLAIQNALIELLDEKLFEKVQVQEIIEKALINRTT
                     FYRYYSGESDLVGKLIKEVKAEYQDLLKKRFESGNLLRFMQTIGNRLLEKRRLILALW
                     QIKTPRHHLYDDMHMLLKNAFIAHAKRITSDDKNWAEKNWDYQAHIFATIALESHKYY
                     FEQGKLLPIPQVFEEWIEMVQVLKSV"
     gene            complement(3537..4526)
                     /locus_tag="PXB27_001741"
     CDS             complement(3537..4526)
                     /locus_tag="PXB27_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012073273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL5346507.1"
                     /translation="MSKQIQFTKTGSPDVLQIVDVAIPAPKAHEVQIQIQAIGLNRAE
                     MMYREGAYVIDPVFPATLGYEGAGVIVAIGEGVNEVAISDKVSIIPSFMFTEYGTYGE
                     IVNMPKHAVVKHPDNLSMEQASASWMAFVTAYGGLIEFGKVQKGDFVVLGGATSSVGL
                     ASIQIAKMQGATVIALSRTHAKGNVLLEKGADFVIATSEDDVTAKLFEITNGKGVNVV
                     FDPVGRKEAAKIINAMAQDGRYIIYGALSHDDIAVPVFPILGKHLTVRGYELFEITTV
                     PEKLAQAKTFVYDGLASGQLRPEIDKIFAFDEMVKAHEYMASNQQIGKIVVKV"
     gene            complement(4740..5657)
                     /locus_tag="PXB27_001742"
     CDS             complement(4740..5657)
                     /locus_tag="PXB27_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL5346508.1"
                     /translation="MTFIIGILAGVLIAFQSAINARLRMHVGAAFLTSFVSFSVGLLF
                     LLGLSLIMQQTPAFSLDNLGNVPLWAWTGGLLGMVGLTANVLIFPKLGGVQTAVMPIL
                     GQVLMGVLIDTFAWLNSPYIAFTASRLFGIGLVLFGVWIAVVLPHLSGLKRKENANLW
                     LWRAVGILGGTALATQAAVNGEFGRQPGSSLGAATVSFGVGAIGLLLVVLFYEKSLPK
                     LCQPTEGKPFWIWSGGILGSLFILAGVWLVPQIGTGSVVMLVLSGLICGSLLVDKFGL
                     FGITQKPILPLQIIGVTLLLAGVAFIRLL"
     gene            complement(5793..6452)
                     /locus_tag="PXB27_001743"
     CDS             complement(5793..6452)
                     /locus_tag="PXB27_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003672168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL5346509.1"
                     /translation="MQFFNKEDVINAFHYRASTRSYDGSRKIPAEDFNYILELGRLSP
                     SSVGSEPWQFLVLQNPELRQKIKPYCWGIPTMETSSHIVVILAKKNARYDSQYFSEIM
                     DRRGLQGEAREKAMAVYKKFQEKDIGILDSERSLFDWASKQTYIALGNMMTGAALIGV
                     DSCPIEGFAYQEVNDILAAEGLFDPSEWGVSVMCTFGYRDKEIRQKARKDFETVVKFI
                     E"
     gene            complement(6637..7701)
                     /locus_tag="PXB27_001744"
     CDS             complement(6637..7701)
                     /locus_tag="PXB27_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HDL5346510.1"
                     /translation="MAKFRKLFTPFNIKNLALKNRVVMPPMCQYSATNGVPNDWHFVH
                     YTSRAVGGVGLIIVEMTNVAPNGRITPNCLGLWNDEQQQAFKKLVDSVHAQGAKIGVQ
                     IAHAGRKAQDEPDAVAPSAIHYGELDYAGQNLITPRELTTDEVKALVQAFKDSVKRAV
                     EAGFDTIEIHGAHGYLIHQFYSPKSNQRSDEYGQDKMLFGEQVIQAAREVMPSEMPLI
                     VRISAQEYGENGFGLDYGLQVAKRFADAGADVLDISGGGDGVLHAGNHPEFYAGYQVD
                     FARQVKLATGKPVIAVGMLDNPAVGDHILGMGDADLVAVGRALLRDPYWVLNAQYDQN
                     AADSSEMQFVPKQYERGLFM"
     gene            7792..8700
                     /locus_tag="PXB27_001745"
     CDS             7792..8700
                     /locus_tag="PXB27_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL5346511.1"
                     /translation="MSDIRTLDFNLLKAFVVLLDECNVSRAAQRLSITQPAMSGILNR
                     LRESFNDPLFVRVQHGMQPTDRALQLGQTARNILQDISSMLQPPILEPEHLNMTLRIA
                     AMDYVQQIIALPLILRLRRLAPNVKVALLPVQGQNIKTLFEQKKIDLALVSRQHLSTD
                     TLKTVLYEERYVCAMSKTHPMAQTPLSLDQFCNLPFAMLSYNGGEFSGATDLALQKIG
                     LQRKVMVSVNHISLLPQLLQGSDLVAVLPERLAKTLPNVYLQPPPIEVEGFTMMMAWH
                     ERTEQDTAHRWLREVLQGVVKPKQAL"
     gene            complement(8770..9321)
                     /locus_tag="PXB27_001746"
     CDS             complement(8770..9321)
                     /locus_tag="PXB27_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL5346512.1"
                     /translation="MAKQIAVLIGSGSKTSFSHLTVRHLQKMAPASIQLNIVDIADLP
                     LYDRDLDENSPAQYTRVREAVAVADGVLLVTPEHNASFSAMLKNAIDVVSRPMGQSKW
                     IGKPAGIVTVGASPAGGVRAADQVRTITSGSFVNMPTLPFALNVGNLFTGVFDEQGEI
                     ASETLKSSMQNFINAYAQFVEKY"
BASE COUNT         2736 a         2114 c         1982 g         2555 t
ORIGIN      
        1 ggagaaaaaa tgcaaacaaa accgatatta gatgcctgtt gtggttcccg tatgtttcat
       61 tttgacaagc aaaatccaca cgttttattt gctgataatc gacaaataga aaccacaatg
      121 aaagacagtg gcaaagtccg ccatttagaa atcaaacctg atgttttaca cgacttcacg
      181 aatatgccat acgaaaacga gagtttttgg cacgttgttt ttgatccgcc acatttagtt
      241 caaggtggag aaaatagctg gcttgtgaag aagtacggca aattaccttg caactggcga
      301 gaagtcttga agaaaggctt tgacgaatgt atgcgagtgc taaaaccaaa cggcacgctt
      361 attttcaagt ggaatgaaac acaaatcccc gtttcagaaa ttttaaaagt tattgattgt
      421 cagccgattt atggacacaa aagcggacgc ttgaaccaaa cacattggct ggcgtttgtg
      481 aaaattgagc aatccctcta aattgagggg gttatttgga gagaatatgg aacaaccgac
      541 tgaattttta acgaaagacg aacttactga tttaacaggc tatcgtcaaa agaaaaggca
      601 aatcgaatgg ttgagggagc gtcactatat aattcaagat gaaaacaagt ataagccatt
      661 agtgctatac aaagatgtct atgggcaccc ccgccaacaa tatccagaag ctcaatccac
      721 tgggaaatgg aaatcacctg cttatttaat gggagtgaaa aatgggaaga ccacgcaaac
      781 aacagaatcg ctacttacct gagaatttac gtccaaaaac agttacacga aaatcaggca
      841 aaactgtcat ttattggcta tatcgcacgc acggaaaaac ggaaatctct ctaggcacaa
      901 atagaaatga ggcttttatt aaggctgctc aactcaatat agaaagagaa agccaaactg
      961 ccagaattac cttcgggctt gtagccaaac gttatcgaga tgaagttata cctcagaaaa
     1021 aagcgagcac agcacaaaca gatttgtgca agataaaaaa tctactgcta ttctttaatg
     1081 acgctcctct cgaagaaatc acccctcaac atatccgaga gtatatgatt tggcgaaaag
     1141 acacacctgg atctgctaat aacgaatttt caatgttcag tcatatttgg caatacgctc
     1201 gagagtgggg ttatacaaac ttaccaagcc ctaatatcgg aatgaaacgc taccctatca
     1261 gaaagcgtga aatttacgtt gagaacaata tatttcagat ggtatatgaa gtggcagatc
     1321 aggatatgag agacttaatg gatctcgcct atatcaccgg acaacgtcct gtcgatttgg
     1381 tcaatatccg gaaagaacac atttttgatg gttatctcca tattgttcag caaaaaacac
     1441 aagccaaact acgtattgag atagtgggaa aactggctga tattatccaa gataggctgg
     1501 agaaagctac tggaacacac ttattcagca ctaagaaagg ggaaaagcta accgctagaa
     1561 aactaggtaa aaggttcact tatattcggg aacaagcggt caagaataac ccggaatacg
     1621 caaatgaaat aatggcattc caatttcggg atctaagagc taaatcagga acagataaag
     1681 ccctttctct aggtgaagaa gccgctcgcc aacaactagg tcatactagc gttcaaatga
     1741 ccaaaacata cattcgaaaa gccccaatag taacccctat caaagaaagt gttccctcct
     1801 caaaacacca gtcttaaaag acagtttcgg aacattttgc attttttcgg aacgtttaca
     1861 taaaaatcac ttataataga ttgaaagata agtaattttt gttactcgtt attactgata
     1921 atatttttaa taaaaatgac cgcttgcctt tcaacaagcg gtcattcccc tttaattttt
     1981 taccaccgta aacaccttat ttttcagccg atattcactt ttttcatcgt gagccagttg
     2041 cacaatggtt ggcttgcgtt tacacaattc acgcattgca cacgccgtca atgttgccag
     2101 caagcatatc ttgttcagag taagaggcat cgccgccaat caaaatataa tgctcgccca
     2161 tatccaccac caccgccaaa tgccccagcg tatgtcctgc ggttggcaca aggcggattt
     2221 tgccgtcttt ggtgagttta tgactgctct caaaactttc aaacgcccca tcattaaaat
     2281 agatgccatc aggcttaaac caatcaggat agtgcattgt taaataaccg ttcatcaccg
     2341 cattgggggc gttgattgcc tgtttttcag tctcactcat cacaaatcag ctgtttggga
     2401 aattgggcaa tccgcctgcg tggtcgccgt gcatatgggt cattaccacc gtgtcaatgc
     2461 gtaaagggtc aaagccgttt gcctttaaaa tttcgcccac ttcttcgtgt tttttgaccc
     2521 cacgccgttc gcaaaactgc ataaacgggt gccaccacgg ctgatagccc ttgtcgttgg
     2581 cacgactgct ttcgcccgta tccaccaaaa tcaaaccgtc atcgtgttca atcagccagc
     2641 aaccaatcgg cagtttaggc gaccattttt tatcggtcag cacatcaagc acacgggcag
     2701 gacgggcaaa aaagcgtgcc acttgatgat gaattttgat ttgtacccaa cctgttgtca
     2761 gacaatgcac tttaatcatt tttgtgttct cattaaaaaa tcgtgggcat attgtagaaa
     2821 gtgcggtaac ttttcacaaa cattaaaaag ttcgtatttg gctggatatg cagataaaat
     2881 tacaaaaatg tatgcttatt ttgaaaaaag atgaacgatt taagagtaat caaaacccat
     2941 ctagcaatcc aaaacgcttt gattgaatta ttagatgaaa agctgtttga aaaagtgcaa
     3001 gtccaagaaa tcattgaaaa agccttgatt aaccgcacca ctttttaccg ttattattcg
     3061 ggcgaaagtg atttggtcgg caaattgata aaagaggtga aggcggaata tcaagacctg
     3121 ctcaaaaagc gttttgaaag cggtaatttg ctgcgattta tgcaaaccat tggcaaccgc
     3181 ttattggaaa aaagacggct gattttggca ctttggcaaa tcaaaacccc tcgccatcat
     3241 ttgtatgacg atatgcacat gctgttaaaa aacgccttta tcgcccacgc caaacggata
     3301 acatcagatg ataaaaactg ggctgagaaa aattgggatt accaagccca tatttttgcc
     3361 accattgccc ttgaaagcca taaatattat tttgaacaag gcaaactttt gcccattcca
     3421 caggtgtttg aggagtggat agaaatggtg caggtgttga aaagtgttta gaaataactg
     3481 gtaaaatttt ccataaaaaa caccgctttc atcacaaaag cggtgctttc aaatcatcaa
     3541 accttaacca caatcttacc aatctgctga ttgcttgcca tatattcgtg agctttaacc
     3601 atttcatcaa aggcaaagat tttgtcaatt tcagggcgta actgaccgct tgccaaaccg
     3661 tcatagacaa aggttttggc ttgtgccaat ttttcaggta cggtggtaat ttcaaagagt
     3721 tcataccccc gcacggtcaa atgcttaccc aaaatcggga acactggcac agcaatatca
     3781 tcatggctta acgctccata aatgatgtaa cgaccatctt gtgccattgc attgatgatt
     3841 tttgccgcct cttttctgcc cacagggtca aataccacat tgacgccttt gccattggtg
     3901 atttcaaaaa gtttggcggt tacatcgtct tcgcttgtgg caatcacaaa atccgctcct
     3961 ttttccaaaa gtacattgcc tttggcgtgg gtgcgagaca aggcaatcac ggttgcccct
     4021 tgcattttgg cgatttggat actggctaaa ccaaccgaac tggttgcacc gcccagcacc
     4081 acaaaatcgc ctttttgtac tttgccaaac tcaatcagtc cgccataagc ggtaacaaac
     4141 gccatccaac tggcggacgc ttgttccatt gacaaattat cagggtgctt gaccacagcg
     4201 tgttttggca tattgacgat ttcgccataa gtgccgtatt cggtaaacat aaaggacgga
     4261 ataatgctca ccttatcgct aatggcaact tcgttcacac cctcgccaat cgccacaatc
     4321 acaccagccc cttcataacc caaagtcgca gggaatacag ggtcaatcac atacgcccct
     4381 tcacggtaca tcatttcggc acggtttaag ccaatggctt ggatttggat ttgtacttcg
     4441 tgtgcttttg gtgcaggaat tgctacatca acgatttgca agacatcagg cgaacctgtt
     4501 tttgtaaatt ggatttgttt gctcattgtt ttgacctttt gtgtaaataa tgggcgtatt
     4561 ttaatgcaat gaatttattt taaacacaac atcaattgca aaacactttt gctaaattta
     4621 cataaataca ttttcacaat gtctgaaaag ttgttttgca aattttaaaa agaaaaccac
     4681 cgcttgccga tcacaagcgg tggtttttgt gggttatttt gcaagaggtg tcagcttggc
     4741 tacagtaagc ggataaatgc gacgccggct aagagtaagg taacgccgat gatctgcagc
     4801 ggtagaatgg gtttttgtgt aatgccgaac agaccgaatt tatctaccag caggctgccg
     4861 caaatcaggc cagagaggac gagcatcacc accgagcctg tgccgatttg ggggacgagc
     4921 caaacgccag cgaggataaa caggctgccc agtatgccgc cgctccatat ccaaaacggt
     4981 ttgccttcgg ttggctggca gagtttcggc aggctttttt cataaaacag cactaccagt
     5041 aataagccga tggcaccgac accgaaggag actgttgctg cgccgagtga tgagccgggc
     5101 tgtctgccga attcgccatt aacggcggct tgggttgcta atgcggttcc gcctaatatg
     5161 ccgactgcgc gccacagcca taaatttgcg ttttctttgc gttttagacc ggacagatgg
     5221 ggcagtacca cggcaatcca tacgccgaat aagactaaac caatgccaaa caggcggctg
     5281 gccgtgaagg ctatgtaagg cgagttaagc caagcgaaag tgtcgatcag cacgcccatc
     5341 agcacttggc ctaaaatcgg catgaccgca gtttgtacgc cgccgagctt ggggaagatc
     5401 agtacgtttg ccgttagccc caccatgccg agtaatccgc ccgtccatgc ccataagggg
     5461 acgttgccta aattgtcgag gctaaatgct ggtgtttgtt gcatgatgag cgagagtccg
     5521 agtagaaaca ggaggcctac gctgaaagag acaaacgagg ttaaaaatgc cgcacccacg
     5581 tgcatacgca aacgggcgtt gatggctgat tgaaatgcaa tcagcacgcc ggctaaaata
     5641 ccgataataa atgtcatgac taactgggtt gtgctgccgc ctcaaaatga gtggtggttg
     5701 aaaagaaatg ccgtctgaat gcggattcag acggccttaa ggcttggtat cggcgtttac
     5761 ctgatgttga atagccgttt tgcctagccg gtttattcga tgaatttcac cacggtttcg
     5821 aaatctttac gtgctttttg gcggatttct ttatcacggt aaccgaaggt acacatcacc
     5881 gatacgcccc attcgcttgg gtcaaataat ccttccgctg ccaaaatatc gttgacttct
     5941 tggtaagcga aaccttctat cggacaagaa tctacgccaa ttaaagctgc accggtcatc
     6001 atattgccca atgcgatgta ggtttgtttg cttgcccagt caaacaggct gcgttcgctg
     6061 tctaaaatgc cgatatcttt ctcttggaat tttttataca cggccatggc tttttcgcgc
     6121 gcttcgcctt gtaaacctcg tctatccata atttcagaaa aatattggct gtcgtaacgg
     6181 gcatttttct tcgctaaaat caccacgata tggcttgagg tttccatagt tggaatgccc
     6241 cagcagtaag gtttgatttt ttgccgtaat tcagggtttt gcaacactaa aaactgccaa
     6301 ggctctgagc ctaccgaact tggacttaaa cgaccgagtt ccagaatata gttgaaatct
     6361 tcagccggaa tttttctgct gccgtcgtaa gaacgggtag atgcacggta atgaaaggca
     6421 ttgattacgt cttctttgtt gaaaaattgc atggaacact cctaaaaatg caaaacagtc
     6481 ttttcagacg gtctgaaaag tctagatgaa atacggataa gtataaaaga tttaagttat
     6541 ttttaataat tgataatttt gatatttagg tataagttga cgtgatgaga aaaaacagac
     6601 cctctgaatg ttcaggcggt ctttaacgtt ggttgtttac ataaataatc cgcgttcata
     6661 ttgcttcggt acaaactgca tttcactgct gtctgctgca ttttggtcat attgcgcatt
     6721 caatacccag tacggatcgc gcaataaggc tcttcccaca gccactaaat ccgcatcgcc
     6781 catacctaag atatgatcgc ctaccgccgg attatccaac atacctactg caattaccgg
     6841 tttgcccgta gcgagcttca cttgacgggc aaagtctact tggtaacctg cgtaaaattc
     6901 ggggtgatta cctgcatgca atacaccatc accaccaccg ctgatgtcta agacatctgc
     6961 gcctgcatcg gcaaaacgtt tcgctacctg caaaccataa tccaagccaa agccgttttc
     7021 accgtattct tgtgcagaaa tgcggacaat taacggcatt tcagacggca tcacttcacg
     7081 tgctgcctga atcacttgct cgccaaacaa cattttgtct tggccgtact catcactgcg
     7141 ctgattggat ttcggcgaat agaattggtg aattagataa ccgtgtgcgc cgtggatttc
     7201 aattgtatca aagcctgcct caacggcacg tttgaccgaa tccttaaagg cttgaaccaa
     7261 tgctttgact tcgtctgtcg tcagctcgcg aggtgtaatc agattttgtc cggcataatc
     7321 caactcaccg tagtgaattg cacttggagc cactgcatcc ggctcgtctt gggctttgcg
     7381 tccggcatgg gcgatttgca caccgatttt tgccccttga gcatgaacgc tgtctaccag
     7441 ttttttgaat gcctgttgtt gttcatcgtt ccataagcct aagcagtttg gcgtaattct
     7501 gccgtttggc gccacattgg tcatctccac aataatcaag ccgacaccac ctactgcacg
     7561 gctggtgtaa tgcacgaaat gccaatcgtt cggtacgccg ttggttgccg aatattgaca
     7621 cattggtggc ataaccacac ggtttttcaa tgctaagttt ttaatgttaa acggggtgaa
     7681 taattttctg aacttcgcca tgttgccttc cttaatgaaa tagattaggt ggaatgataa
     7741 ttcagtgcta ttatctattc aaattgatta ttgtgatagt taggtataaa aatgagtgat
     7801 ataagaacac tggatttcaa tttgctcaaa gcttttgtgg tgctgctgga cgaatgcaac
     7861 gttagccggg cagcacaacg attatccatc acccaaccgg caatgagcgg tattttaaac
     7921 cgcttgcgcg agagttttaa cgatccgtta tttgtgcgag tgcaacacgg tatgcagcca
     7981 accgaccgcg cgttgcaact ggggcaaacc gcacgcaaca ttctgcaaga tatcagctcc
     8041 atgctgcaac cacccatatt ggaaccggaa catctgaata tgaccttgcg tattgccgca
     8101 atggattatg tacaacaaat tattgccttg ccgctgattc tgcgtttaag acggcttgct
     8161 ccgaatgtaa aagtcgcctt actgccggtg caggggcaaa atatcaaaac tttgttcgag
     8221 caaaaaaaaa tcgatcttgc cttggtcagc cgccaacatt taagcacgga tacgctgaaa
     8281 acggtattat atgaagaacg ctacgtatgc gcgatgagca aaacacaccc gatggcgcaa
     8341 acaccgctat cgctcgatca attctgcaat cttcccttcg caatgctttc ttacaacggc
     8401 ggagaattca gcggagctac ggatctagcg ttacaaaaga taggactaca gcgcaaagta
     8461 atggtctcgg taaaccatat ttccctgctg ccccagctgc tacaaggttc cgacttagtc
     8521 gcagtattgc ccgaacgctt agcaaaaacc ttacccaatg tttatttaca acctccgcct
     8581 atagaggtag aaggcttcac catgatgatg gcttggcacg aaagaaccga acaagatacg
     8641 gcgcatcgat ggttaagaga ggtgctgcag ggtgtggtaa aacctaaaca ggctctttaa
     8701 ttattctttc ttttgctatg aaaaaagccg tttatatttt tagataaacg gcttttttgc
     8761 attctaatat tagtattttt ctacaaactg agcataggca ttaatgaagt tttgcattga
     8821 gctttttaat gtctcagagg caatttcacc ctgttcatca aatacgcctg taaataagtt
     8881 tcctacgttt aatgcgaaag gtaaggttgg catatttaca aatgagcctg aagtaatagt
     8941 acgtacttga tcagctgcac gtacaccgcc tgccgggctt gcgccaacgg ttacgatacc
     9001 tgccggtttg ccaatccatt tgctttgccc cattgggcga gaaaccacgt caatcgcatt
     9061 tttaagcatt gcagaaaagc ttgcattatg ctcaggtgtg actaataaca cgccatctgc
     9121 cacagcaacc gcttcacgca ctctggtgta ttgtgccggg ctgttttcat ctaaatcacg
     9181 gtcgtaaagt ggtaagtctg caatatccac aatgtttaat tgaatgcttg caggtgccat
     9241 tttttgtaag tgacgaacag tcaagtgact aaatgaagtt ttgctgccgc taccaattaa
     9301 tactgcaatt tgttttgcca ttttataatt ccttagtaca tagagaatat aggcatttta
     9361 tccctttcaa tctgtttcat aaagcgc
//
LOCUS       DANLUQ010000096         9373 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.096, whole genome shotgun sequence.
ACCESSION   DANLUQ010000096 DANLUQ010000000
VERSION     DANLUQ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9373
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.096"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(86..370)
                     /locus_tag="PXB27_001747"
     CDS             complement(86..370)
                     /locus_tag="PXB27_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346513.1"
                     /translation="MIREVIDFIKNNKWYVLLVVLLLGLYFYQFHGGLSSDSEKWDHF
                     GSYIGGIFSAVTLLSVLHGMQLERKRFKEQKEEFENDKRVLEKRRRKEDF"
     gene            complement(465..1553)
                     /gene="hisB"
                     /locus_tag="PXB27_001748"
     CDS             complement(465..1553)
                     /gene="hisB"
                     /locus_tag="PXB27_001748"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018355873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HDL5346514.1"
                     /translation="MQPTLFIDRDGTLIDEPKTDFQIDSLEKLKFEKNVIPALLKLKG
                     KYRFVMVTNQDGLGTSSFPQADFDKPHNAMMALFNSQGIEFDDVLICPHKPEDGCDCR
                     KPHTKLLQKYIDRQLFDPANSFVIGDRATDVQLAENLGIRALQYHPEKLNWDLIAEKL
                     LGEAVTNIGERPKRFAEVIRKTKETDIKVQVWLDETGNNEISTGVGFFDHMLDQIATH
                     GGFRMNITTKGDLWIDEHHTVEDTALALGTALKQAIGDKRGIARFGFVLPMDESRAEC
                     AMDLSGRPFIKFKADFKRDKVGDFSTELTEHFFQSIAFTMLATLHIKAKGDNDHHKIE
                     SLFKVFGRTLRQCIRIEGNELPSSKGVL"
     gene            complement(1738..2805)
                     /gene="hisC"
                     /locus_tag="PXB27_001749"
     CDS             complement(1738..2805)
                     /gene="hisC"
                     /locus_tag="PXB27_001749"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDL5346515.1"
                     /translation="MTISQLSRKNIQALTPYQSARRLGGSGDVWLNANEYALSPNFDL
                     TDRTFNRYPEPQPQAVIEGYARYAGVAPENVLVSRGGDESIELIIRAFCEANDSILYC
                     PPTYGMYAVSADTCGIATKTVPLTADFQLDLAQIKANLEGVKVVFVCSPNNPTGNLIK
                     RSDLFELLQITAGKAIVVVDEAYIEFCPEARLVSELANFPHLAIIRTLSKAFALAGLR
                     CGFTLANAELIGVLQKVIAPYPLPVPVSDIAAQALSPQGIEQMKTRVAEVITLRAELQ
                     KNLENLPLVEQVFDSEANYLLFKCQDGQKVFKALWEQGIILRDQHKALGLQNCIRITV
                     GAREENLRVVEAITALSQFQR"
     gene            complement(2835..3275)
                     /locus_tag="PXB27_001750"
     CDS             complement(2835..3275)
                     /locus_tag="PXB27_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL5346516.1"
                     /translation="MSINISPLASKNLTEWKLLWQQYLDFYQVNLADDIIQSTWQKVS
                     ESENVKGFGAFVDNRMVGFVHIVIHPNTWNLTECCYLEDLFVLAEYRNQGIGKELIEY
                     IYRYAQEKGYNRVYWVTDKNNHQAQQLYNKLANDSGIIQYRKNF"
     gene            complement(3342..3719)
                     /locus_tag="PXB27_001751"
     CDS             complement(3342..3719)
                     /locus_tag="PXB27_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HDL5346517.1"
                     /translation="MKNPIEIYQSQDGKTQVSVQFDKETVWLSLQQMADIFGRDKSVI
                     SRHLRNIYQEGELEREATVAKNATVQIEGAREVVRLKDYLVKGYALNERRLQERGIEF
                     EQVIGLLSQYTGCLSLVGGRKFA"
     misc_feature    complement(3741..3863)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            complement(3993..4982)
                     /gene="rsmC"
                     /locus_tag="PXB27_001752"
     CDS             complement(3993..4982)
                     /gene="rsmC"
                     /locus_tag="PXB27_001752"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HDL5346518.1"
                     /translation="MLSLESEVLERHLALFENKSVLLFGDVRDDFARQLQSRAEKVAV
                     FSSYFDYARHHSEVEFGLECGLSAELAVFYWTKNKQECQFQLLQWLSQCQAGQEMLII
                     GENRAGVRSVEKLLEPFGNIAKIDSARRCGLYHFELQTVPTFDCKKFWKSYRLQDLDV
                     FDLPAVFSSAELDNGTKLLLSTFDKADRLKGKVLDLGCGAGVIGATLKQQFPKIKLTM
                     SDIHAMALESGRRTLAENQLEGEVVASDVFSHINERFDLIVSNPPFHDGVDTAYRAVE
                     ELIFQAKNHLTKGGELRIVANAFLPYPDLLDKAFGKHDVLAKSTKFKVYSART"
     gene            5112..5534
                     /locus_tag="PXB27_001753"
     CDS             5112..5534
                     /locus_tag="PXB27_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HDL5346519.1"
                     /translation="MNRRDLLLQEMGISQWVLTNPQVLKGDAQIRLNNAVKLVVVCEE
                     DHQHSRLFADILRALKLKNSEYQWLTGEQSQRLVFEHSPIFWLIQAEEQAVKMAKSFT
                     NQTAWKNESWQDLAKSTTKRQLWQQIDTFSAQFGEQRD"
     gene            5527..5964
                     /gene="rimI"
                     /locus_tag="PXB27_001754"
     CDS             5527..5964
                     /gene="rimI"
                     /locus_tag="PXB27_001754"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDL5346520.1"
                     /translation="MIEQIQAEDFEQLFEIEQQAHLVPWSKGTLLNNQGEKYLNLKVV
                     EQNKIVAFAISHIVLDEATLFNIAVAPDFQGKGIGKRLLSELILQLQQRKLTTLWLEV
                     RESNHSARKLYDSLGFNEVTTRKNYYPTPNGSKENAVVMALYL"
     gene            complement(6013..7860)
                     /gene="typA"
                     /locus_tag="PXB27_001755"
     CDS             complement(6013..7860)
                     /gene="typA"
                     /locus_tag="PXB27_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDL5346521.1"
                     /translation="MQNVDISKLRNIAIIAHVDHGKTTLVDKLLQQSGTFGETRGDVD
                     ERVMDSNDLEKERGITILAKNTAINWNGYHINIVDTPGHADFGGEVERVLSMVDSVLL
                     VVDAFDGPMPQTRFVTQKAFAHGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLGASD
                     EQLDFPIIYASALNGVAGLEHEDLAADMTPLYEAIVKHVQPPQVELDAPFQMQISQLD
                     YNNYVGVIGIGRIKRGSVKPNQAVTIVDSEGKTRQGRIGQVLGHLGLQRFEATEAFAG
                     DIVAITGLGELNISDTICDINAVEALPALSVDEPTVTMFFCVNTSPFCGQEGKFVTSR
                     QILERLNKELVHNVALRVEETSNPDEFRVSGRGELHLSVLIENMRREGYELAVSRPKV
                     IYREEDGKKQEPFEQVTIDIEEQHQGSVMEALGIRKGEVRDMIPDGKGRTRLEYVIPS
                     RGLIGFRNEFMTMTSGTGLLYSTFSHYDDVKPGEIGQRKNGVLISNATGKALAYALFG
                     LQERGKLMIDHGVDVYEGQIIGIHSRSNDLTVNCLQGKKLTNMRASGKDDAIVLTTPV
                     RFTLEQALEFIDDDELVEVTPQSVRIRKRLLTENDRKRASRTTTSTSTH"
     gene            complement(8045..8722)
                     /locus_tag="PXB27_001756"
     CDS             complement(8045..8722)
                     /locus_tag="PXB27_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDL5346522.1"
                     /translation="MSIAENLSQIHQQIQQISEQYQRDNVRLLAVSKTKPVEAIQAAI
                     EAGQRAFGENYVQEAVEKIEFFANRPELEWHFIGPLQSNKTKLVAAYFDWIQTVDRLK
                     IAERLNEQRPADKAPLNVLIQINISDEASKSGIEPDEMLPLAQAISQLPNLKLRGLMA
                     IPKPESEPEQQKVAFRKMQLLFNRLQTEFDDIDTLSMGMSDDMQAAIECGSTMVRIGT
                     AIFGARR"
     gene            8793..9332
                     /locus_tag="PXB27_001757"
     CDS             8793..9332
                     /locus_tag="PXB27_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HDL5346523.1"
                     /translation="MLDLTKIKLVITDVDGVLTDGGMYYTDQGEVMKRFHVHDGLGVK
                     MLQSCGIKVAVLSGGDTALLRKRLEVLKIDLALLGKMEKRSACFELMQQAGVTPEQTA
                     YIGDDTLDLPAFEVCGIAIATRNALDYIKEKADWVLQKAGGEGAFREVSDQILEAQGF
                     GEIFKTADGFLTVAGKMAQ"
BASE COUNT         2522 a         2109 c         1973 g         2769 t
ORIGIN      
        1 ttagcatatc ataaacatta tcaatattac cttctattga tgaaatttta agattatgct
       61 gctctaacat cataaaaaat gtacgctaaa aatcttcttt tcttcttctc ttttctaata
      121 ctcttttgtc attttcaaat tcttcttttt gctccttaaa tcttttacgt tccaactgca
      181 ttccatgcaa aacactcaat aaagtaacag cagaaaatat accaccaata tagctaccaa
      241 aatgatccca tttttcacta tctgaggata atcccccgtg aaactgataa aaatataatc
      301 ctaataacaa tacaactaac agaacatacc atttattatt ctttataaag tcaataactt
      361 ctctaatcat caatatatcc tttataacaa gcggtcaaaa tccccaaaaa ctttgcaaat
      421 ttcaaccgca ctttcgggcg ggttaaaacc cgcccctacg gtgattataa tacaccttta
      481 ctgcttggta gttcattccc ttcaatacga atgcactgtc tgagtgttct gccgaagact
      541 ttgaacaggc tttcgatttt gtggtggtcg ttgtcgcctt ttgccttgat gtgcagggtg
      601 gcgagcatcg tgaaggcgat ggattggaag aagtgttcgg tgagctcggt gctgaaatcg
      661 cccactttgt cgcgtttgaa atccgctttg aacttgatga aaggtctgcc tgataagtcc
      721 atcgcacatt ccgcacggct ttcgtccatc ggcaacacga agccgaaacg ggcgatgccg
      781 cgtttgtcgc caatcgcttg ttttaatgcg gtgccgaggg cgagggcggt gtcttccacc
      841 gtgtgatgtt cgtcaatcca caagtcgcct ttggtggtga tgttcattct aaaaccgccg
      901 tgggtggcaa tttggtcaag catatggtcg aaaaagccca cgccggtgct gatttcgttg
      961 ttgcctgttt cgtccagcca cacttgcact ttgatgtcgg tctctttggt tttacggata
     1021 acttcggcaa agcgctttgg tcgctcgcca atgttggtga cggcttcgcc cagtagtttt
     1081 tcagcaatca aatcccagtt cagtttttca gggtgatatt gcaacgcccg aatgccaagg
     1141 ttttccgcca gttgcacatc ggtagcacgg tcgccaatca caaagctgtt ggctgggtcg
     1201 aacagttggc ggtcgatata tttttgtagc agtttggtgt gcggtttgcg gcaatcgcaa
     1261 ccgtcttcgg gcttgtgtgg acaaatcagc acatcatcaa attcaatccc ttgcgagttg
     1321 aacaacgcca tcattgcatt gtgcggtttg tcgaaatctg cctgtgggaa cgaactcgtg
     1381 cctaagccgt cttggttcgt aaccatcaca aagcggtatt tgcccttgag tttgagcagg
     1441 gcaggaatca cgtttttctc gaatttcagc ttttccaagc tgtcgatttg aaaatcggtt
     1501 tttggctcat caattaacgt gccgtcacgg tcgataaaaa gggtgggttg cattggtttc
     1561 tcctgtatct gtatcttatt cattactctc cccacacgcc aaggcatatg gggttaagca
     1621 ttagtgagag actttgcctc tctgtattca gtcccaaagg gactgactta tgcgtaacct
     1681 agtacggcta tgccgtgcta ggctataaat gtctcttaaa atactgctcg ttaaattcta
     1741 tctctgaaat tggctcagtg ctgtaatcgc ctcaaccacc ctcaaattct cctctctcgc
     1801 ccccaccgta atccgaatgc aattttgcaa tcctaacgct ttgtgttggt cacgcaaaat
     1861 aatgccttgc tcccaaagtg ctttgaagac tttttgtccg tcttggcatt tgaataggag
     1921 gtagttggct tcgctgtcaa aaacttgttc tacaagcggt aagttttcca gatttttttg
     1981 caattcggca cgcagggtga tgacttccgc cacacgggtt ttcatctgct caatgccttg
     2041 tggggagagg gcttgggcgg cgatgtcgga aacaggcaca ggcagcggat acggggcaat
     2101 cactttttgt aacacgccga tcagctcagc gtttgccaag gtaaagccac aacgcaaacc
     2161 cgccaaggca aaggctttgg atagggtgcg gataatcgcc aaatgtggga aattcgccag
     2221 ctcgcttacc aagcgagctt cagggcaaaa ttcgatgtag gcttcgtcca ccaccacaat
     2281 cgctttgcct gcggtgattt gcaaaagttc gaacaaatcc gaccgcttga ttaagttgcc
     2341 tgtcgggttg ttcgggctgc acacaaacac cactttcacg ccctcgaggt tagctttgat
     2401 ttgggctaaa tccaactgga aatcggcggt taaaggcaca gttttggtcg caatgccaca
     2461 ggtgtcggca ctcacggcgt acattccgta ggttggtggg cagtagagaa tgctgtcgtt
     2521 tgcctcgcaa aaggctcgga taatcagctc aatgctctca tcgcccccac gggaaaccag
     2581 cacgttttca ggtgcaacgc ctgcatatcg ggcgtaacct tcaatcaccg cctgcggttg
     2641 aggttcaggg tagcggttaa aagtgcggtc ggttaaatca aagtttggcg aaagggcata
     2701 ttcattggca ttcagccaca catcgccact accccccaaa cgccgtgccg attggtaagg
     2761 ggtgagagct tgaatgtttt tacgggaaag ttgtgaaatt gtcatattgt gttcctgtat
     2821 aatttataca tagtttagaa attttttcga tattgaataa taccgctatc attagctaat
     2881 ttattataaa gctgttgtgc ttgatgattg tttttatctg ttacccaata tactcggtta
     2941 tatccttttt cctgtgcata tcgataaata tattcaatta attcttttcc tatgccttgg
     3001 ttgcgatatt cagctaaaac aaataaatct tccaaataac agcattcagt taaattccaa
     3061 gtatttgggt gaatcacaat atgaacaaaa ccaaccattc tgttatcaac aaaagcccca
     3121 aatcccttta cattttcact ctctgaaact ttctgccaag ttgattgaat aatatcatct
     3181 gctaaattca cttgataaaa atctaaatat tgttgccaca atagtttcca ttcagtgaga
     3241 tttttggagg ctaacggcga aatattaata ctcatatttt ttcctgttat gcttttaata
     3301 aaataaagtg ttcaatcagt ttaatcatca gttctttttg ttcaggcaaa ctttctgcca
     3361 ccaacaaggc taagacaacc agtgtattga ctgagcaaac caatcacctg ctcaaactcg
     3421 atacctcgtt cctgcaaacg gcgttcgtta agggcatagc ctttcaccag atagtctttt
     3481 aggcgaacga cttctctagc accttcaatt tgaactgttg cattttttgc aacagttgcc
     3541 tcacgctcca gttcaccttc ttggtagata tttcgtaagt gccgagaaat aacagactta
     3601 tctcgtccaa aaatatctgc catttgctgt aaacttagcc acaccgtttc cttatcaaac
     3661 tgcacggaaa cttgggtttt gccgtcttgg ctttggtaaa tttcaatcgg gtttttcata
     3721 ttattcccaa attttatcta taaaaaaatc tctcgaaagt tgccttccga gagatcttta
     3781 ttgatttgct acacttgctc ggaagagtta tcttccgcac acaccaaatg cccgaaagat
     3841 atcaggttaa agtgtggtgg tgatgtaaag tgtagtttaa attcattgca ttactccaaa
     3901 acagtatgaa cagaatttac gtcaagccgg cagaaatatc aagcactttt ttaagcaaat
     3961 accggctttt ttataaccgt ttgttctgcc tattatgttc ttgcggaata caccttaaac
     4021 ttggtggatt ttgccagtac gtcgtgtttg ccgaaggctt tatcgagcaa atctgggtaa
     4081 ggcaagaatg cgtttgcgac aattcgcagc tcaccgcctt tggttaggtg atttttagct
     4141 tggaaaatca actcctccac cgctcggtag gcggtatcga ccccgtcgtg gaatggtggg
     4201 ttggagacaa ttaaatcaaa ccgttcatta atgtgagaga acacatcact tgctaccact
     4261 tccccctcta actgattttc cgccaaagta cgtctgccgg attccaacgc cattgcgtga
     4321 atatcgctca tcgtcagttt gattttcgga aattgttgtt tgagcgttgc cccaatcacg
     4381 ccagccccac agcctaaatc taacactttg ccttttagcc tgtctgcttt gtcgaaggtt
     4441 gaaagcaata atttggtgcc gttatccagc tccgccgagc taaaaaccgc tggtagatcg
     4501 aacacatcta agtcttgcaa gcggtaggat ttccagaatt ttttgcaatc gaatgtcggc
     4561 acggtctgta gctcaaaatg gtataaacca caacggcgtg cagagtcgat tttggcaata
     4621 ttgccgaacg gttcgagcag tttttctacc gaacgaacac cagctcggtt ttcgccgata
     4681 atcaacattt cttgccctgc ttggcattgc gacagccatt gtagcagttg gaattggcat
     4741 tcctgcttgt tcttcgtcca ataaaacacc gctaactcgg cactcaaacc acattctaag
     4801 ccaaattcaa cctcactatg gtggcgtgcg taatcaaaat agctactgaa taccgcaacc
     4861 ttttccgcac gagattgcag ctggcgggca aaatcatctc gcacatcgcc aaataataac
     4921 accgacttgt tttcaaataa cgccaaatgg cgttcaagta cttcgctttc taaagagagc
     4981 attttcttac cttatctaaa aaatttagcg tgaatatacc gaatttaagt acagaaatct
     5041 tgaatttttt gaaaaaagtt tctgttcaag cggagttttg atactatttg caaaatttga
     5101 tgaggtttta aatgaatcgt cgtgatttac tgttacagga aatgggtatt tcgcaatggg
     5161 tgctaaccaa tccccaagtg ctaaaaggcg atgctcaaat ccgcctaaac aatgcggtaa
     5221 aattggtggt agtgtgtgag gaagatcacc aacacagccg gctctttgcc gatattctgc
     5281 gtgctttaaa gttaaaaaac agcgaatatc aatggctgac tggcgaacaa tcacaacgtt
     5341 tggtgtttga gcattcgccg atcttttggc tgattcaagc ggaagaacaa gcggtcaaaa
     5401 tggcaaaaag ttttactaat cagaccgctt ggaaaaacga gtcttggcaa gatttagcca
     5461 aatctacgac caaacgccaa ctttggcaac aaattgatac tttttcggca caatttggag
     5521 aacagcgtga ttgagcaaat tcaagcggaa gatttcgagc agttatttga aattgagcaa
     5581 caagcccatt tagtgccgtg gtcaaagggg acgttattaa ataatcaggg tgaaaaatac
     5641 ctgaatttaa aagtggtaga gcaaaacaaa attgtggctt ttgccattag tcatatcgtg
     5701 ctagatgaag caaccttatt taacattgca gttgctcctg attttcaagg caaaggcatt
     5761 ggcaagcggt tactttccga attaattttg caactgcaac aacggaaact caccaccctg
     5821 tggttggaag tgcgagaatc caaccattct gccagaaaac tctacgattc actcggtttt
     5881 aatgaagtta ccacccgcaa aaactattac cccacgccaa acggtagcaa agaaaatgcg
     5941 gtggtaatgg cgttatattt gtagggagaa tttaaaacca aaagagcgac ctcagtcgct
     6001 cttttttatc agttagtgcg tgctggtact ggttgtagta cggcttgcac gtttccggtc
     6061 gttttcagtt aataaacgtt tacgaatacg caccgattgt ggtgttactt ccactaattc
     6121 gtcatcgtca ataaactcta acgcttgttc tagagtgaaa cgcaccggtg tggttaaaac
     6181 gatggcatcg tctttacctg atgcacgcat attggttaat ttcttaccct gtaagcagtt
     6241 tacggttaaa tcgtttgaac ggctgtgaat cccgataatt tgcccttcat atacatcaac
     6301 accgtggtcg atcattaatt ttccacgctc ttgtaagcca aataatgcgt atgctaatgc
     6361 cttacccgtt gcgttagaaa ttaacacacc gtttttacgc tgaccgattt cgcccggttt
     6421 tacgtcatca tagtggctga atgtagagta aagtaaacca gtacctgagg tcatcgtcat
     6481 aaattcgtta cggaaaccga ttaagccacg gcttgggatc acatactcta aacgggtacg
     6541 acctttgccg tccggaatca tatcacgcac ttcaccttta cggataccaa gtgcttccat
     6601 cactgaacct tggtgttgct cttcgatatc aatcgtcact tgctcaaacg gctcttgttt
     6661 tttgccgtct tcttcgcggt agattacttt cgggcgagac actgctaact cgtaaccttc
     6721 acgacgcata ttttcgatta ataccgataa gtgtaattcg ccacgacctg acacacggaa
     6781 ttcgtctggg ttcgaagttt cttccacacg caatgccacg ttgtggacta attctttgtt
     6841 taaacgctca agaatttggc gtgaagtcac aaatttacct tcttgaccgc agaatggcga
     6901 ggtgtttacg cagaagaaca tcgttaccgt tggttcatca acgcttaatg ctggtaaagc
     6961 ctctactgcg ttaatatcac aaatagtatc tgaaatattt aattcgccta aaccagtaat
     7021 cgcaacaata tcgccagcaa aggcttctgt tgcttcaaaa cgttgtaaac ctaaatgccc
     7081 caacacctga ccgatacgac cttggcgagt tttgccttcg ctatctacaa tcgttaccgc
     7141 ttggttaggt ttcaccgaac cacgtttgat acgtccaata ccgataacgc ccacatagtt
     7201 gttgtagtct aattgcgaaa tctgcatttg gaatggtgca tctaattcca cttgtggtgg
     7261 ttgaacgtgt ttgacaatcg cttcataaag tggggtcata tctgccgcta ggtcttcgtg
     7321 ttctaagcct gctacaccat ttaaggctga agcgtagata attgggaaat ctaactgctc
     7381 atcacttgca cctaaattta caaacaagtc gaacacttga tccaccaccc aatcagggcg
     7441 agcacctggg cggtcaactt tattgatcac tacgattggt tttaaaccgt gagcgaacgc
     7501 tttttgcgtg acgaaacggg tttgcggcat cgggccatca aaggcatcaa ccactaacag
     7561 aacagaatct accattgaaa gtacacgctc aacctcacca ccgaagtcag cgtgccctgg
     7621 ggtatctacg atattgatgt gataaccatt ccagttaatt gccgtatttt ttgcaagaat
     7681 ggtaatgcca cgctcttttt caagatcgtt ggaatccatt acacgctcat caacgtcacc
     7741 acgagtttca ccaaaggtgc ctgattgttg taaaagttta tcaaccaagg tggttttacc
     7801 gtggtcaacg tgggcaataa ttgcaatgtt acgcaatttt gaaatatcta cattttgcat
     7861 tggaatatct ttaagttaag tcaaaataaa aactctcgcc catcaaaact ggcgagagtc
     7921 aagaaattag gaggcgtaat tatacagact ttttccgctg ttggctacaa aacaagcggt
     7981 ggaatttgca aagtttttgg cgaatttgac cgcttgtatc agctttatgt aaaaactcag
     8041 ctggctaacg tctcgcccca aaaatcgcag tcccaatccg caccattgtc gagccacatt
     8101 caatcgccgc ttgcatatcg tccgacatcc ccattgaaag ggtatcaatg tcgtcaaatt
     8161 ctgtttgcaa gcggttaaaa agtagctgca ttttgcgaaa ggcaactttt tgttgctctg
     8221 gctcgctttc tggtttggga atggccatta agccccgcag tttcaaattc ggtaattgag
     8281 aaatcgcttg ggcaagtggt agcatttcat ctggttcaat gcccgatttc gaggcttcat
     8341 cgctgatatt aatttgaatc agcacgttga gcggggcttt atccgctggg cgttgttcgt
     8401 ttaaacgctc ggcaattttt aatctatcca ccgtttgaat ccaatcaaaa taggcagcga
     8461 ccaatttggt tttatttgat tgaagtggcc caataaaatg ccattctaat tcggggcgat
     8521 ttgcaaaaaa ttcgattttt tcgaccgctt cctgcacata attttcgcca aatgcacgct
     8581 gccctgcctc aatcgccgct tgaatcgcct caactggttt agttttggag acggctaaca
     8641 aacgcacgtt atctcgttga tattgttcgg aaatttgctg aatttgttga tggatttgcg
     8701 ataaattctc ggcaatcgac atttcggttc ctttggtaaa cggaggtaaa aaagctataa
     8761 tggtaaaatc ataccatatt tgaggcaaaa aaatgttaga tctcaccaag attaaattag
     8821 tgattaccga tgtggacggc gtgctaaccg atggcggaat gtactacacc gaccaaggcg
     8881 aagtaatgaa acgtttccac gttcacgatg gtttaggcgt taagatgtta caaagttgtg
     8941 gcattaaagt ggcagtactc tcgggcggcg atacggcttt gctccgcaaa cggttagagg
     9001 tgctgaaaat tgatctcgct ttgttaggca aaatggaaaa acgctccgcc tgctttgagc
     9061 taatgcagca agccggtgtt acccctgagc aaacggctta cattggtgat gacaccttag
     9121 atttaccggc ttttgaagtg tgtggcattg cgattgcaac ccgtaacgcc ctcgattaca
     9181 tcaaagaaaa agcagattgg gtattgcaaa aagccggtgg tgaaggggcg tttcgtgaag
     9241 tctccgacca aattttggag gctcaaggat tcggtgaaat ttttaaaacc gcagatggtt
     9301 ttttaacggt agcggggaaa atggctcagt agactcttca agcagttaaa tttcatttta
     9361 tttttgcgat ctg
//
LOCUS       DANLUQ010000097         9357 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.097, whole genome shotgun sequence.
ACCESSION   DANLUQ010000097 DANLUQ010000000
VERSION     DANLUQ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9357
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.097"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..1285)
                     /locus_tag="PXB27_001758"
     CDS             complement(<1..1285)
                     /locus_tag="PXB27_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDL5346524.1"
                     /translation="MDSIPLSSLFITLAILLVLSAFFSGSETGLMSLNRYKMRHLAEQ
                     GHKGAKLAEKLLARTDVLLSLILICNNLVNIAASAIATMIGMQLSGEAGVAIATGLLT
                     FVMLVFSEILPKTVAAIYPEKVGFFASFVLTPLKKLLSPIVFLMNLIIAGLMKLLRIQ
                     QKDKQGLSSEELRSVVLDAGKFIPAEHREMLVSILDMEKVTVDDIMVPRNDISGIDID
                     DDWKAIMRQLNHAPHARVIIYKGDMDNNIVGILRVREAFRLMLEKNEFTKEMLLRATD
                     EVYFIPEGTPLTTQLMNFKANKERIGLVVDEYGDIKGLVTLEDILEEIVGEFTTSSAP
                     TLEQEVQQQSDGTVIIEGSANLRDLNKLFDWDLPLEEARTFNGLILEHLEKIPDEGME
                     FELYNLKVMILEVSENMVKQAKVEHLSPKIEKEQDS"
     gene            complement(1409..2206)
                     /gene="ccsA"
                     /locus_tag="PXB27_001759"
     CDS             complement(1409..2206)
                     /gene="ccsA"
                     /locus_tag="PXB27_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HDL5346525.1"
                     /translation="MLLPLLSIVAYALALLWVAPTLVNLENAATVNKKPNMMLVFGFG
                     LLATILHFSVLYNELFLIEGQNFTVTNVISLMSFIMAACVTLALLKWKTVWFPLIIVY
                     SFAICSVGVAGFVKGSFIRNVQENAGLLFHLGIALFSYALFFLALIYAFQLKWLDRKL
                     KSKTMFFCQILPPLMTVERHFFTLTLAAQAMLTLTLFSGVVYLHNFFEPEHIHKAIFS
                     FLAWLVYSIQLLGQWKLHWRGKRVLIYSISGMMLLTIGYFGSRLIVN"
     gene            2422..2946
                     /gene="yceD"
                     /locus_tag="PXB27_001760"
     CDS             2422..2946
                     /gene="yceD"
                     /locus_tag="PXB27_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005621677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HDL5346526.1"
                     /translation="MQKVKLPLTIDPYKDAQRRVDYEGYISGKLLTRLGESVCNVQSD
                     AQVTLSLYVDPQRLTVIKGTAKVDVEFDCQRCGDPFTQTLDCSFVFSPVSNMDQADIL
                     PEIYEPIEVNEFGEVNLLDMIEDEFILELPLVPMHSEEHCEVSVSEQVFGELPEELVQ
                     KPNPFAVLANLKKN"
     gene            2963..3133
                     /gene="rpmF"
                     /locus_tag="PXB27_001761"
     CDS             2963..3133
                     /gene="rpmF"
                     /locus_tag="PXB27_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006231206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDL5346527.1"
                     /translation="MAVQQNKKSRSRRDMRRSHDALTTAAVSVDKTSGETHFRHHVTA
                     DGYYRGRKVINK"
     gene            3152..4174
                     /gene="plsX"
                     /locus_tag="PXB27_001762"
     CDS             3152..4174
                     /gene="plsX"
                     /locus_tag="PXB27_001762"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDL5346528.1"
                     /translation="MVDITLAVDVMGGDFGPRVTIPALEKALKQNPSLSFLLFGDEHQ
                     IAPLLQKHTLLNHPKIQIHHTEQVIEADVPFTTAIRQSKNSSMRLAIEAVKEGKAQGC
                     VSGGNTGALMGLAKLLIEPLPNIERPALTSLIPTLNGKSSVMLDLGANIEVSNHQLQQ
                     FAEMGNLFAQVMLDLVYPRLSLLNIGTEENKGTAQLQAVHQLLKNRQDLNYTGFIESD
                     KLTSYLTDVIICDGYTGNIALKALEGAAKNIISLFKKEKADSNICRNTKRYLLKAMFY
                     RYYRKLQQINPDRHNGATLLGLSKVVVKSHGGANINAFYYAIDYAVQQIQGDIPNKIL
                     EGLTQK"
     gene            complement(4271..5554)
                     /gene="hisD"
                     /locus_tag="PXB27_001763"
     CDS             complement(4271..5554)
                     /gene="hisD"
                     /locus_tag="PXB27_001763"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HDL5346529.1"
                     /translation="MQTLIWNNLTETEQQQYLARPAQVVGESIKAAVNEIKANVIANG
                     DKALFELAEKFDKVKLESLVVSEQQIQQAQSRISPELKQAIQNAKANIEAFHKAQQNQ
                     EVDIETQPGVCCQVITRPINAVGLYIPGGSAPLFSTVLMLAVPAKIAGCNKIVLCSPP
                     PIADEILYTAHLCGVETIYAVGGAQAIIAMANGTQTVAKVDKIFGPGNAFVTEAKRQV
                     VQEGTAIDMPAGPSEVLVIADEFADPAFVASDLLSQAEHGADSQVILVTPSESLARAV
                     DEELEKQVAVLPRAETARKALAHSRTFIAENLQQAVEISNKYAPEHLIVQTENARSLL
                     ADLDNAGSIFLGAYSPESMGDYASGTNHVLPTYGYTKTYSSLGLADFSKRMTVQELTP
                     QGFLALAETVERMASAELLDAHRNAVSLRVAKLKG"
     gene            complement(5566..6465)
                     /gene="hisG"
                     /locus_tag="PXB27_001764"
     CDS             complement(5566..6465)
                     /gene="hisG"
                     /locus_tag="PXB27_001764"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HDL5346530.1"
                     /translation="MTTQNRLRIAMQKKGRLSQECSELLKQCGVKIIWNDQRLIAYSE
                     NMPIEILRVRDDDIPGLVFDGVVDLGIIGENVLEEVELGRLSAGENVAYKKLRQLDFG
                     GCRLSLAIDRDKNYNNIKDLEDAVIATSYPNLLKRYMKEQNVPFKSILLTGSVEVAPS
                     AGIANAICDLVSSGATLEANGLKEVEVIYRSTACLIQRQEPLSAEKQALVDKLLTRIQ
                     GVQQANESKYIMLHAPKDKLAEITALLPGVENPTILPLANDQTRVAMHVVSQENLFWE
                     TMEQLKEMGASSVLVLPIEKMLA"
     misc_feature    complement(6498..6625)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            6824..7681
                     /locus_tag="PXB27_001765"
     CDS             6824..7681
                     /locus_tag="PXB27_001765"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDL5346531.1"
                     /translation="MQISLKKIYSGKVRDLYEIDEKRMLMVATDRLSAFDVILDDPIP
                     RKGEILTQISNFWFNKLAHIMPNHFTGDSVFDVLPQEEAEAIQHRAVVCKRLIPVKIE
                     SIVRGYLTGSGLKDYQQTGTICGLTLPEGLVEASKLPQPIFTPSSKAEVGDHDINISY
                     EECERQIGQELAAKVRDAALKLYTEAANYALTKGIIICDTKFEFGLDENGELTLMDEV
                     LTPDSSRFWSVETYREGTNPPSFDKQFVRDWLENSGWNKQPPAPKIPAEVIEKTVAKY
                     QEALDLLTK"
     gene            complement(7822..9303)
                     /gene="ilvC"
                     /locus_tag="PXB27_001766"
     CDS             complement(7822..9303)
                     /gene="ilvC"
                     /locus_tag="PXB27_001766"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDL5346532.1"
                     /translation="MANYFNTLNLRQKLDQLGRCRFMDRDEFADGCNFLKGKKIVIVG
                     CGAQGLNQGLNMRDSGLDIAYALRAEAIAEKRASFKRASENGFVVGTYEQLIPEADLV
                     INLTPDKQHSKVVADVMPLMKQGAAFGYSHGFNIVEEGEQIRPDLTVVMTAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGLAIAKAWASATGGDRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSIVCYDKLVSEGKDPAYAGKLIQYGWETITEALKQGGITLMMDR
                     LSNSAKLRAFELSEQIKEELAFLFEKHMDDIISGEFSSTMMADWANGDANLLKWREET
                     GKTAFENAPQADGIKISEQEYFDNGVLMVAMVKAGVELAFDTMVSAGIYEESAYYESL
                     HELPLIANTIARKRLYEMNVVISDTAEYGNYLFSHVATPILAEKIIPTLEKGDLGEPT
                     PAVEIDNVTLRDVNDAIRNHPIELIGQELRGYMTDMKRISSQG"
BASE COUNT         2777 a         2131 c         1770 g         2679 t
ORIGIN      
        1 ctgaatcttg ctctttttca attttcggtg aaagatgttc aactttcgct tgtttaacca
       61 tattttcact cacttcaaga atcatcactt tcaagttgta gagttcaaat tccattcctt
      121 catcaggaat tttttctaaa tgctctaaaa tcaagccgtt aaaggttctg gcttcctcaa
      181 gcggtaaatc ccaatcaaat agtttgttca gatcacgcag gttggctgag ccttcaataa
      241 tcaccgtacc atctgattgt tgctgaactt cttgctcaag ggtcggtgct gatgaagtcg
      301 taaattcacc cacaatttct tctaaaatat cttcaagagt gactagccct ttaatatcac
      361 catactcatc aaccactaaa ccaatacgct ctttattcgc tttaaagttc attaattgtg
      421 tagtgagtgg tgtgccttca ggaataaaat agacttcatc tgttgcacgc agtagcattt
      481 ctttggtaaa ttcgtttttc tccaacatta aacgaaaggc ttcacgcacc cgcaaaatcc
      541 caacaatatt gttgtccata tcccctttgt agataatcac ccgagcgtga ggggcgtgat
      601 taagctgacg cataatcgct ttccagtcat catcaatatc aatgccgctg atgtcgttgc
      661 gaggcaccat aatgtcatca accgtcactt tttccatatc taaaatagag accaacattt
      721 cacggtgttc ggcaggaata aatttaccgg catcaagcac tacactacgc agctcttcgg
      781 agcttaagcc ttgtttatct ttttgttgaa tccgcaataa tttcatcagc ccggcaataa
      841 tcaaattcat taaaaataca atcgggctga gtaatttttt aagcggtgtt agcacaaagc
      901 tggcgaaaaa accgactttt tcagggtaaa ttgccgctac tgttttaggt aggatttccg
      961 aaaaaaccaa cattacaaaa gttagcaaac cggttgcaat tgccacaccg gcttcaccgg
     1021 ataactgcat accgatcatc gttgcaatcg cagaggcggc aatgttgact aagttattac
     1081 aaattaagat aaggctaagt agcacatcgg tgcgtgccaa gagtttttcc gccagtttcg
     1141 cccctttatg cccttgctcg gcaaggtgac gcattttgta gcggtttaaa gacatcaagc
     1201 cggtttccga gcctgaaaaa aatgcagaga gtactaaaag aatagctaag gtaataaata
     1261 aactactcag gggaatactg tccaaaattc aattcctatg taaattaatc ataaaaggta
     1321 gcgtaacact aaaagcgttg tcactacctc actttgtcgg ttcagccgac ttgcaaaaaa
     1381 ttttgctttt ttgaccgctt gtagatcatt aattgacgat caaacgacta ccgaaatagc
     1441 cgatggtgag caacatcata cccgaaatcg aataaataag cacccgtttt ccacgccaat
     1501 gtaatttcca ctgtccaagt aactgaatgc tataaacaag ccaagctaaa aaggagaaaa
     1561 ttgctttatg aatatgctcc ggctcgaaaa agttatgcaa ataaaccaca ccggaaaata
     1621 aagtgagcgt cagcatagct tgtgcagcta aggttaaagt aaagaaatgg cgttcaaccg
     1681 tcattagcgg tggtagaatc tggcaaaaga acattgtttt gctctttagt tttctatcca
     1741 gccatttgag ttgaaaagca taaattaatg ctaaaaagaa gagagcataa gaaaataatg
     1801 caatccctaa atggaaaagc agtcccgcat tttcttgcac attacggata aaactacctt
     1861 ttacaaatcc agccaccccc acactacaaa tggcgaaaga gtacacaata attaaaggaa
     1921 accaaaccgt tttccatttt aacaacgcaa gtgtgacaca agccgccata atgaaactca
     1981 tcaaagagat tacatttgtt accgtaaaat tctgcccttc gattaaaaat aattcattat
     2041 ataacacaga aaaatgcaaa atcgtggcaa gtaacccaaa cccaaagact aacatcatat
     2101 taggcttttt attaactgtt gcggcatttt ctaaattcac tagagtaggg gcaacccaaa
     2161 gtagcgctag agcgtaagca acaatggata aaagaggaag taacatattg tctcgaatct
     2221 cgtcaaataa gaataactat cagaatttta atcaaaagta accgttttag ctagtttttg
     2281 ctgttgctag atcaaaaatt atcctaaaaa atgtgctttt cgccttaaat tgaaccctac
     2341 cttttctcgg taagattatc tcactgaaaa caataatttt cctttgacta atttgctttc
     2401 tatctgtaat atgcacaccc tatgcaaaag gtaaaactac cccttacgat tgacccttac
     2461 aaagatgctc agcgtcgcgt tgattatgaa ggctacattt caggtaaact tctaacacgc
     2521 ttgggtgaat cagtatgtaa tgtgcaaagt gatgcacaag tcactctctc gttatatgtc
     2581 gatccacaac gcctaactgt gattaaaggc acagcaaaag tagatgtgga atttgattgc
     2641 caacgatgtg gtgacccgtt tacacaaacg ctcgactgtt catttgtttt cagtccggtg
     2701 tctaacatgg atcaggcgga catcttgccc gaaatttatg aacctatcga agtaaacgag
     2761 ttcggtgaag taaatttact agatatgatt gaagatgaat ttattcttga attacctcta
     2821 gtcccgatgc atagtgaaga acactgtgaa gtgtccgtaa gtgaacaggt atttggcgag
     2881 ttgccggaag aactggtaca aaaaccaaac ccgttcgcag tattagctaa tttaaagaaa
     2941 aactagatct aggagaaagc ccgtggctgt tcaacaaaat aagaaatctc gttcacgtcg
     3001 tgatatgcgt cgttcacacg atgcattaac aactgcagca gtttcagtag ataaaaccag
     3061 tggtgaaact cactttcgcc accacgtaac tgcagacggt tactaccgtg gtcgtaaagt
     3121 aatcaacaag taattccttt cgaggtttat cttggttgat atcactcttg cagtagatgt
     3181 gatgggcggg gactttggtc cccgcgttac tattcctgca ctggaaaaag ccttaaaaca
     3241 aaatccaagc ctttcttttt tattatttgg tgatgaacac caaatagccc ctttattaca
     3301 aaaacacaca ctactcaacc accccaaaat ccaaattcat cataccgaac aagtgattga
     3361 agctgatgtt ccgttcacta ctgcaatccg ccaaagcaaa aacagctcta tgcggttagc
     3421 gattgaggca gtaaaagagg gcaaagcaca aggttgtgtt agtggcggta acactggtgc
     3481 gttaatggga ttggcaaaat tattgattga accattacca aatattgaac gccctgcact
     3541 cacttcgcta attcctaccc tcaacggcaa atccagcgta atgttagatt taggggcaaa
     3601 tattgaagta agcaatcacc agctacaaca atttgcagaa atgggaaacc tgtttgctca
     3661 agtaatgctt gatttagttt accctcgcct gtctttattg aatattggta cagaagaaaa
     3721 taaaggcacg gcacaacttc aggcagtgca tcaactgctg aaaaacagac aagacttaaa
     3781 ttacaccggc tttattgaaa gcgataaatt aaccagctat ctcactgatg tgattatttg
     3841 tgacggctac accggcaata tcgcgttaaa agcgttggaa ggggcagcta aaaatattat
     3901 ttctctcttt aaaaaagaga aagccgattc caatatttgc cgtaacacca aacgatattt
     3961 actgaaagcg atgttctacc gttactaccg caaattgcaa caaattaacc ctgaccgcca
     4021 taacggtgcg accttgctcg gcttatctaa agtagtcgtg aaaagccacg gcggggcaaa
     4081 tatcaatgcc ttttattacg ctattgatta tgcggtgcaa caaattcaag gcgacattcc
     4141 aaataaaatc ctagagggtt taacccaaaa ataagcgaac tcaactgttc gcttattttc
     4201 atttcttgtt tcacttaaag atacaagcgg tcaaatttgc aaaaaaattt acccattcaa
     4261 ccgcttgtgt ttagcctttt aattttgcca ctcgtaaact aaccgcattg cggtgtgcat
     4321 ctagcaattc tgcacttgcc attcgttcca ccgtttcggc caaggctaga aagccttgtg
     4381 gtgtcagctc ctgcaccgtc attcgcttgc taaaatcagc taaacctaag ctcgaatagg
     4441 ttttagtgta gccgtaagtc ggtaaaacgt ggttagtgcc gctagcataa tcccccatac
     4501 tttccggcga atacgcccct aagaaaatcg agccggcatt atctaaatcc gccaacaacg
     4561 agcgagcatt ttcagtttgc acaattaaat gctcgggtgc atatttatta ctaatttcga
     4621 ccgcttgttg caggttttcc gcaataaaag tgcgactgtg agccaatgct ttacgggcag
     4681 tttcggcacg aggtaaaacc gccacctgct tttctaattc ttcatcgact gcccttgcca
     4741 agctctcact tggtgtgact aaaatcactt gggaatcggc tccatgttct gcttgagaga
     4801 gtaaatcgct cgccacaaat gcagggtcag caaactcatc ggcaatcacc aacacttccg
     4861 acgggcctgc cggcatatca atcgccgtgc cttcttgcac aacttggcgt ttcgcctccg
     4921 tcacaaacgc attgcccggg ccgaaaattt tatccacttt cgctaccgtt tgtgtgccat
     4981 ttgccatcgc aataatcgct tgagcccccc caactgcgta aatcgtttcc accccacaca
     5041 aatgggcggt gtagagaatt tcatcggcaa tcggcggtgg tgagcaaagc acaatcttgt
     5101 tacaaccggc aatttttgcc ggcactgcaa gcattaacac ggtagagaaa agcggggctg
     5161 aaccgcccgg aatataaagc cccaccgcat taatcgggcg agtgatgact tggcaacaca
     5221 cccccggctg ggtttcaata tccacctctt gattttgctg tgccttgtgg aaagcctcga
     5281 tattcgcttt cgcattttga atcgcttgct taagctctgg tgaaatacgg ctctgtgctt
     5341 gctgaatctg ctgttctgat acaactaagc tctctaattt tactttatcg aacttttcag
     5401 ccaattcaaa taatgcctta tcgccgttgg caattacatt cgctttaatt tcattaactg
     5461 ccgctttaat actctcgcca accacctgtg ccggacgggc aagatattgc tgctgctcag
     5521 tttctgttaa attgttccaa attagggttt gcatatttat tccttttacg ccaacatttt
     5581 ctcaatcggt aaaaccaata cggaacttgc gcccatttct tttagctgct ccattgtttc
     5641 ccagaacagg ttttcttggc tgacaacgtg cattgccaca cgggtttggt cgttagctaa
     5701 cggtaaaata gtcgggtttt ccacaccggg taataaagcg gtaatttccg ccagtttgtc
     5761 tttgggggcg tgcagcataa tatacttaga ttcgttcgcc tgttgcacac cttgaatgcg
     5821 ggttaataat ttatccacta acgcttgttt ttccgctgaa agtggttctt ggcgttgaat
     5881 taggcaagca gttgaacggt aaatcacttc aacctctttt aatccgttcg cctctaatgt
     5941 tgcacctgaa gaaaccaagt cacaaatcgc attcgcaatt ccggcacttg gagcaacttc
     6001 aaccgaaccg gtgagtaaaa tacttttaaa cggcacattt tgctctttca tataccgctt
     6061 taataaattg gggtaagaag tggcaatcac agcatcttct aaatctttga tattgttata
     6121 atttttatca cgatcaatag ctaacgataa acggcagcca ccaaaatcta actgacgaag
     6181 ttttttatag gcaacattct caccagcact taatcgccct aattccactt cctctaatac
     6241 attttcaccg ataatcccaa gatcaactac gccgtcaaaa actaagcctg gaatgtcgtc
     6301 atcacgcacc cgtaaaattt caatcggcat attttctgaa taggcaatca ggcgttgatc
     6361 gttccaaatg attttcacgc cacattgttt aagcaactca ctacattctt ggcttaagcg
     6421 accttttttc tgcatcgcaa ttcttaatcg attttgtgtt gtcattgttg tttccttctt
     6481 atgttttaaa ttgtaaaaat aaaaaaacct ctcgaaatca cttccgagag ggttaaattt
     6541 atgctgcgtt taggcgtact cggaagtctt atcttccgca cataccaaat gcccgaaaga
     6601 ttattcaggt aaatgatggt ggtgatgagt acgaataaaa gccattttgt tgttctccaa
     6661 actaaattgc tttaaaagat acgcttaaat tttggcaatg acaagtgttt tttgcgaaaa
     6721 aattgctatt tttcatctct ctaaaacaac tatcgcaaac gtttgcgtaa agcggtataa
     6781 tttgcctgat tttttgcaaa ttcaacaaag taggaataac actatgcaaa tcagcctcaa
     6841 aaagatctac tccggcaaag tccgtgatct ctacgaaatc gatgaaaaac gtatgcttat
     6901 ggttgccacc gaccgattat ctgcctttga tgttatttta gacgatccta ttcctcgtaa
     6961 aggcgaaatc ctcacccaaa tctccaactt ctggttcaac aaattagccc acattatgcc
     7021 aaaccacttc accggcgaca gtgtgtttga cgtgttaccg caagaagagg cagaagctat
     7081 tcaacatcgt gcggtggttt gcaaacgctt aataccggtg aaaattgaat cgattgtgcg
     7141 gggttattta accggctccg ggctgaaaga ttaccaacaa accggcacga tctgcggctt
     7201 aacattgccg gaaggcttgg ttgaggccag caaattaccg caaccgatct tcacaccgtc
     7261 cagcaaagcc gaggtgggcg atcacgatat taatatcagc tacgaagagt gtgaacgcca
     7321 gatcggtcaa gaacttgccg ccaaagtgcg tgatgccgct ctaaaactct acactgaagc
     7381 ggcaaactat gccctgacca aaggaattat tatttgcgac accaaattcg aattcggctt
     7441 agacgaaaac ggtgaactaa cgctaatgga cgaagtccta accccggatt ccagccgttt
     7501 ttggtcagtt gaaacctacc gagaaggcac aaatccacct tcatttgata aacaattcgt
     7561 gcgtgactgg ctggaaaata gcggttggaa caaacaaccc ccagccccga aaataccggc
     7621 agaggtaatt gaaaaaaccg tagcgaaata tcaagaagca ttggatttat taaccaaata
     7681 acaccacaag cggtcaaatt tacgctattt tttgcaaaac aaaaacctgt aagccaacac
     7741 ttacaggttt tttagttagt ggtaaaaagt gcggttattc taccgcactt ttgcaaaatt
     7801 ttgtgtaaat ctgaccgctt gttagccttg cgaagaaata cgtttcatat ctgtcatata
     7861 accacgtaat tcttgaccga ttaactcgat tgggtggtta cggattgcgt cattcacatc
     7921 acgtaaggta acgttgtcga tttccactgc cggagttggt tcacctaaat cgcctttctc
     7981 taacgttggg ataattttct ccgctaaaat cggggttgca acgtgagaga ataagtagtt
     8041 accgtattct gcggtatctg aaattaccac gttcatttca tataaacgct tacgagcgat
     8101 tgtatttgcg attaacggta actcgtgtag agactcgtag taagccgatt cttcataaat
     8161 accggctgaa accattgtat caaaggctaa ttcaacccct gctttcacca ttgcaaccat
     8221 taacacaccg ttatcaaagt attcttgctc agaaatttta ataccgtctg cttgtggtgc
     8281 gttttcaaag gctgttttac ctgtttcttc acgccatttt aataagttgg catcgccgtt
     8341 tgcccagtct gccatcatcg ttgatgagaa ctcaccactg atgatgtcgt ccatatgttt
     8401 ttcaaataag aacgctaatt cttctttgat ttgctcagaa agctcgaacg cacgcaattt
     8461 ggcactgttt gataagcgat ccatcattaa ggtgataccg ccttgtttta acgcttcggt
     8521 aatggtttcc caaccgtatt ggatcaattt acctgcgtaa gccggatctt taccttccga
     8581 cactaatttg tcgtagcaca cgattgaacc tgcttgtaac ataccgcaaa gaatggtttg
     8641 ttcacccatt aaatcagatt tcacttctgc tacgaatgaa gattctaata cgcccgcacg
     8701 gtcaccaccg gtagcacttg cccacgcttt cgcaatggct aaaccttcgc ctttagggtc
     8761 attttccggg tgaacggcaa ttaaggttgg cacaccgaaa ccacgtttgt actcttcacg
     8821 cacttccgtg cccgggcatt tcggagcggt catcacaacc gttaaatccg gacgaatttg
     8881 ctcaccttct tccacaatgt tgaaaccgtg tgaataacca aatgccgcac cttgtttcat
     8941 taacggcatt acatcggcaa ctactttaga gtgctgtttg tccggcgtta agttaatgac
     9001 taaatccgct tcaggaatta attgctcgta agtacccacc acaaagccgt tttcagaagc
     9061 acgtttaaat gaagcacgtt tttcagcaat cgcttcggca cgtaaagcgt aagcaatatc
     9121 taagcctgaa tcacgcatat ttaaaccttg gtttaaacct tgagcaccac aacccacaat
     9181 cacgattttt ttgcctttta agaaattgca gccatctgca aattcatcac gatccataaa
     9241 gcggcaacga cctaactggt ctaatttttg acgtaaattt aaagtgttga aatagttagc
     9301 cattttttaa tccttatttg aattaatgag attgatatga tgttttgttt gcacatt
//
LOCUS       DANLUQ010000098         9313 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.098, whole genome shotgun sequence.
ACCESSION   DANLUQ010000098 DANLUQ010000000
VERSION     DANLUQ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9313
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.098"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            39..254
                     /locus_tag="PXB27_001767"
     CDS             39..254
                     /locus_tag="PXB27_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HDL5346533.1"
                     /translation="MFPEFRDLITQLKQNNSHFARLFDKHNELDQRIKNMEANIELAT
                     HEEVEALKKEKLHLKDQLYAILKQESA"
     gene            complement(313..1470)
                     /locus_tag="PXB27_001768"
     CDS             complement(313..1470)
                     /locus_tag="PXB27_001768"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HDL5346534.1"
                     /translation="MSLDLADIRTQITNLDRNLLKLLAERHRLAFDVVRSKEITQKPL
                     RDLEREKILLQELVNFAEAENYQLDPQYVTQIFQRIIEDSVLTQQNYLQNKLNAQKEE
                     QVSIAFLGMRGSYSNMASRQFAKKYQGTLAELSCHSFNEVFEKVSLGEADFGVLPLEN
                     TTSGSINEVYDLLQHTDLTLVGELAYPIKHCVLANGTVDLAEVDTIYTHPQPAQQCSQ
                     FLAGLDKVHIKYCESSSHAMQMVARLNKPNIVALGNEDGGKLYGLTNIKTDIANQAHN
                     ITRFIVVAKEAVQVSPQVQTKTLLLMTTTQQAGALVDALMVFKNHHIRMMKLESRPIY
                     GKPWEEMFYVELEANIHSENTQKALAELEQVTSYLKVLGCYPSEIIEPVKL"
     gene            complement(1592..3658)
                     /gene="prlC"
                     /locus_tag="PXB27_001769"
     CDS             complement(1592..3658)
                     /gene="prlC"
                     /locus_tag="PXB27_001769"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HDL5346535.1"
                     /translation="MSNPLLNYTGLPAFSQIKPEHIKPAVEAVIKECRETVEKVAQIE
                     NPTWENFYMPQAMAEDKFSRAWSPVGHLNAVKNSPELREAYQACLPLLSEYSTWAGQH
                     QGLYQGYVKLKNSPEFETYSVAQKKAIENSLQDFELSGISLPAEKQKRYGEISARLSE
                     LNSQFSNNVLDATMGWDIVITDEAQLKGLPESALEAAKLSAESKGKKGYRFTLEIPSY
                     LPVMTYCENKALREEMYRAYVTRASDQGPNAGKWDNSAVIDEILKLRHEKAQLLGFKT
                     YSDYSLATKMAENPQQVLDFLNDLAARSKAQGKNELCDLKKFAKAHFGIEHLDLWDIP
                     FYSEKQKQALYSVNDEELRPYFPENRVLSGLFEVIKRIFGMRVEEQKDVDVWHKDVRF
                     FNIFDKNDRLRGSFYLDLYARENKRGGAWMDDCINQKRLADGTLQKPVAYLTCNFNKP
                     IGDKPALFTHDEVTTLFHEFGHGIHHMLTEIDVGDVAGINGVPWDAVELPSQFLENWC
                     WEEEALAFISGHYETGEPLPKAKLEQLLAAKNFQAAMFVLRQLEFGLFDFRLHTEYDP
                     SKQGQVLALLKEVKDQVAVAKGVEWARTPHSFSHIFAGGYAAGYYSYLWAEVLSADAY
                     SRFEEEGIFNPETGQSFLDNILTRGGSEEPMVLFERFRGRKPTLDALLRHKGIVGNAE
                     PVDCTQ"
     gene            complement(3789..4529)
                     /gene="pflA"
                     /locus_tag="PXB27_001770"
     CDS             complement(3789..4529)
                     /gene="pflA"
                     /locus_tag="PXB27_001770"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HDL5346536.1"
                     /translation="MSVVGRIHSFESCGTVDGPGIRFILFLQGCLMRCKYCHNRDTWD
                     LDGGKEITVEELMKEVTTYKHFMKATGGGVTASGGEAVLQMEFVRDWFRACKAEGINT
                     CLDTNGFVRHYSPVVDEMLEVTDLVLLDLKQLNDEVHQDLVGVSNKRTLDFARYLHKL
                     NKPTWVRYVVVPGYTDDDDSAHRLGQFIQGMDNIEKVELLPYHRLGAHKWETLGYKYE
                     LEGVMSPPKEDLERIQKIIESYGHTVKF"
     gene            complement(4778..5803)
                     /locus_tag="PXB27_001771"
     CDS             complement(4778..5803)
                     /locus_tag="PXB27_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HDL5346537.1"
                     /translation="MSKQDLIRSSVAEYLTFITATGESQVDAVYADENVWLSQKMMAV
                     LYDVDVRTISYHLKKIFSDNELAENSVIQFFWITAQDGKNYHTKHYNLSAIIAVGYKV
                     NSERAVQFRKWATEIVHSFAIKGFAMDDERLKNDGTILGKKYFEEQLARIREIRLSER
                     KFYQKITDIYATAVDYDRTATATKRFFATVQNKLHWAIHGHTAAELIMARANAEKPNM
                     GLTNWKDAPQGKIYPFDVVVAKNYLSDNELAQLQRLVSAYLDMAEDMAERQIPMTMAD
                     WETRLNRFLAATDREILQDAGKVTAEIAKSFALSEFEKYRVKQDLTYESDFDLLVKEV
                     AAKYHAG"
     gene            complement(6038..8362)
                     /gene="pflB"
                     /locus_tag="PXB27_001772"
     CDS             complement(6038..8362)
                     /gene="pflB"
                     /locus_tag="PXB27_001772"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDL5346538.1"
                     /translation="MTQLTDAQQKAWEGFAAGEWQTEVNVRDFIQKNYTPYEGDESFL
                     AEVTPATSKLWADVMEMIKVENKTHEPYDIDCDTPSTITSHAPGYIDKSLEKIVGLQT
                     DAPLKRAIMPFGGINMVKGSCKVYRRELKPEVEQIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVITGLPDAYGRGRIIGDYRRMALYGADFLMKDKQRQFASLQARLESGEDIQATI
                     QLREEIAEQHRALGKMKEMAASYGFDISGPATNAQEAVQWTYFAYLAAVKSQNGAAMS
                     FGRVSSFLDIYIERDLKAGKITEQEAQELIDHLVMKLRMVRFLRTPEYDQLFSGDPMW
                     ATETLAGMGLDGRTLVTKNSFRILHTLYTMGPSPEPNLTILWSEQLPEAFKRYCAKVS
                     IDTSSVQYENDDLMRPDFQNDDYAIACCVSPMIVGKMMQFFGARANLAKTLLYAINGG
                     VDEKSGDQVGPKTEAITSEYLDYDDVMTRLDSFMDWLATQYVTALNIIHFMHDKYAYE
                     AALMALHDRDVFRTMACGIAGLSVAADSLSAIKYAKVKPIRTDIEIKNKAGEVVGVAK
                     NVATDFEIEGEYPQFGNNDNRVDDIAVDLVERFMKKIQKLKTYRNATPTQSVLTITSN
                     VVYGKKTGNTPDGRRSGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTF
                     SIVPNALGKDYEAQKRNLAGLMDGYFHHEATVEGGQHLNVNVLNRDTLLDAVEHPEKY
                     PQLTIRVSGYAVRFNSLTKEQQMDVITRTFTESM"
     gene            complement(8452..9276)
                     /gene="focA"
                     /locus_tag="PXB27_001773"
     CDS             complement(8452..9276)
                     /gene="focA"
                     /locus_tag="PXB27_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HDL5346539.1"
                     /translation="MFSPAEMAKIAEDGAVYKATKNQLYSFLSAITAGGFIAIAFVFY
                     TTTQVGASQMPWGMAKLIGGLVFSLGVIMCVVFGAELFTSSTLTIVARASQRINWLQM
                     FKNWVVVYFGNFVGAISIVLLIWFSGQIMAAKGQWGLVILNTAQHKIHHQWFEAFCLG
                     IFCNIMVCIAVWMSYAGKSLTDKAFIMMLPIAMFVASGFEHCVANMFMIPMGIAISHF
                     ASPEFWTAINVDPTQFADLDVSHFVFKNLIPATLGNIVGGVFFIGLMQWFLYIRKH"
BASE COUNT         2616 a         2106 c         1837 g         2754 t
ORIGIN      
        1 aatcaagtaa aatttgcaac cactattagg agttaaatat gtttccagaa tttcgtgatc
       61 tcataaccca attaaagcaa aacaattcac attttgctcg cttatttgat aaacataatg
      121 agctagacca gcgtattaag aatatggagg caaatattga actggcaaca catgaagaag
      181 ttgaggcact aaaaaaagaa aaattgcatt taaaagacca actttatgca attctaaaac
      241 aagaatcggc ttaacctaaa gtaaaagctc agaaaatatt ctgagcttta atttcggtca
      301 tattagacaa tattacagtt ttaccggctc aataatttcg cttgggtagc aacccaacac
      361 tttgaggtag ctggtaactt gctccaattc cgccaatgct ttttgggtgt tctcggaatg
      421 aatatttgcc tccagctcca cataaaacat ctcttcccaa ggtttgccgt aaatcgggcg
      481 ggattccagt ttcatcatac gaatatgatg atttttgaat accattaacg catcgaccaa
      541 tgcacccgcc tgttgcgtgg tggtcattaa cagtaacgtt ttggtttgta cctgtggcga
      601 aacctgcaca gcctctttgg caacaacaat aaaacgggtg atattgtgag cttgattagc
      661 aatatcggtt ttgatattcg tcaagccata caattttccg ccatcttcat tgcccaaggc
      721 aacaatattc ggcttattca agcgagccac catttgcatt gcgtgcgagc tgctttcgca
      781 atatttgata tgtactttat ctagcccggc taaaaattgg ctacattgct gggcaggctg
      841 agggtgggtg taaatggtat ccacctccgc caaatcaacc gtgccgtttg ccaatacaca
      901 atgtttgatt gggtacgcca attcacccac taaggttaaa tcggtatgct gcaataaatc
      961 atacacttca ttgatcgaac ctgaagtcgt gttttcaagc ggcagtacgc caaaatcggc
     1021 ttcaccaagg ctgactttct caaatacttc attaaaagaa tggcagctta attccgccaa
     1081 tgtgccttga tattttttgg caaactgtcg agatgccata ttagaataag agcctcgcat
     1141 tcccaaaaac gcaatcgaaa cctgctcttc tttttgtgca ttcaatttat tttgcagata
     1201 attttgctga gtcagcactg aatcttcaat aatacgttgg aaaatttggg tcacatattg
     1261 cggatcaagc tggtaattct ccgcttcggc aaaatttacc agttcttgaa gcaaaatctt
     1321 ttcccgttct aaatctctca agggtttttg tgtgatttct ttgctccgca ccacatcaaa
     1381 ggctaagcgg tggcgttctg ccagtagctt taataaattg cggtctaagt tggttatttg
     1441 ggttcgaata tcggctaaat ctaaagacat tatttcactc tctgtcaggc taaatgctga
     1501 aaattataac gagttgcaaa agaaagaaaa gacaagcggt caaatttaca aaaaaatttg
     1561 caaatctaac cgcttgtaaa acgtgtttac tttattgagt gcaatcaaca ggctcggcat
     1621 tacctacaat gcctttgtgt ctgagcaagg catcaagggt tggcttacgc ccacggaagc
     1681 gttcgaacag caccattggc tcttctgaac cgcctcgggt taagatatta tctaagaaag
     1741 actgccccgt ttcagggttg aaaatcccct cttcctcaaa gcgtgaatag gcatctgctg
     1801 aaagcacttc cgcccataaa taactgtagt agcccgccgc atagccgcct gcaaaaatat
     1861 gcgagaaact gtgtggcgtt ctcgcccact ctacgccttt ggcaacagcg acttggtctt
     1921 tcacctcttt aagtaaggct aacacttgtc cttgtttgct cgggtcatat tcggtgtgta
     1981 ggcggaaatc aaacaagcca aattctaatt ggcgaagcac aaacattgcc gcttggaagt
     2041 ttttcgccgc caataattgc tctaatttcg cttttggtaa cggctcgccg gtttcataat
     2101 gccctgaaat aaaggctaat gcctcttctt cccagcacca gttctctaag aattggcttg
     2161 gcagctccac tgcgtcccac ggcacaccgt taatgccggc gacatcgccc acgtcaattt
     2221 cagttagcat atggtgaatg ccgtgtccaa actcgtggaa caaagttgtc acttcatcgt
     2281 gggtaaacaa tgccggttta tcgccaatcg gtttattgaa gttacaggtt aaatatgcca
     2341 ccggtttttg taatgtgccg tcagccaagc gtttttggtt gatgcaatca tccatccacg
     2401 ccccaccacg tttgttttca cgagcgtata aatctaagta gaacgagcca cgcaagcggt
     2461 catttttatc aaaaatattg aaaaaacgta catctttgtg ccatacatcc acatcttttt
     2521 gttcttccac acgcataccg aaaatgcgtt taattacttc aaataagcct gataatacgc
     2581 gattttccgg gaaatacggg cgaagttctt catcattgac cgaatacaat gcctgttttt
     2641 gtttttcgct gtagaacgga atatcccata aatccaaatg ttcaatgccg aaatgggctt
     2701 tagcaaattt tttcaaatcg cataattcat ttttgccttg agctttcgag cgagcggcta
     2761 aatcgttcaa gaaatctaat acctgttgtg gattttctgc cattttggtg gctaatgaat
     2821 aatcagaata agtcttaaag ccgagaagtt gggctttttc gtgacgcagt tttaagatct
     2881 catcaatcac cgcactgtta tcccatttgc ctgcattcgg gccttgatca gaagcacgag
     2941 tcacataagc acggtacatt tcctcacgca gtgctttgtt ttcacaatac gtcatcaccg
     3001 gcaaatagct tgggatttca agagtgaagc gatagccttt cttacctttg ctttctgcgg
     3061 ataattttgc cgcttctaat gccgattccg gcaagccttt aagctgtgct tcatcggtaa
     3121 ttacaatatc ccaacccatt gttgcatcaa gtacgttgtt gctaaattgc gagttgagtt
     3181 ccgacaagcg tgcagaaatt tccccgtagc gtttttgttt ctctgccggc agtgaaatgc
     3241 ctgataattc aaaatcttgt aagctgtttt caatcgcctt tttctgtgca accgaataag
     3301 tttcaaactc agggctgttt tttaacttca catagccttg atataaccct tgatgttgcc
     3361 ccgcccaagt gctgtattcc gataataacg gcaagcacgc ttggtaagcc tcacgcaact
     3421 ctggggaatt tttcaccgca ttcaaatgcc ctaccggtga ccacgcacgg gagaatttat
     3481 cctctgccat tgcttgtggc atatagaaat tttcccacgt cgggttttca atttgtgcga
     3541 ctttttcaac agtttcacgg cattctttga tcaccgcttc taccgccggt ttgatatgtt
     3601 caggtttgat ttgtgagaac gcaggcaagc ctgtgtaatt taaaagaggg ttagacatta
     3661 ttttttcctt ttatggtcaa aatgaaaatt cgtatcaaga taatacacaa agtgagagag
     3721 aaaagaaaac aagcggtcat atttcaataa ttttttgcaa aaaaatcaca aaatatgacc
     3781 gcttgtcttt agaatttgac tgtatgcccg taactttcaa tgattttttg gatacgctct
     3841 aaatcctctt taggcggtga cattacccct tccagctcat atttatagcc taaggtttcc
     3901 catttgtgag cccctaaacg atgataaggc agaagttcca ctttctcaat gttatccatt
     3961 ccttgaataa actgccctaa acgatgggca gaatcatcgt catcagtata acccggcacg
     4021 accacatagc gaacccaagt cggtttattt aatttgtgca gataacgggc gaaatcaagg
     4081 gtacgcttat tcgacacccc gactaaatct tggtgaactt catcattgag ctgttttaaa
     4141 tccagcaaga ccaaatcggt cacttcaagc atttcatcca ccaccgggct ataatggcga
     4201 acaaaaccat tggtatctaa gcaggtatta atgccttcag ccttacaggc acgaaaccaa
     4261 tcccgcacaa actccatttg caacaccgct tccccacccg aagctgttac accaccgccg
     4321 gttgctttca taaaatgctt ataagtcgtg acttctttca tcagctcttc aaccgtaatt
     4381 tccttaccac catcaagatc ccaagtatca cgattatggc aatatttaca ccgcatcaaa
     4441 cagccttgca agaacagaat aaaacgaatc cccggtccat ctaccgtacc gcaggattca
     4501 aaagaatgga tacgtccaac aacagacatt tgtcacctca accctcaaaa aattttgaga
     4561 aattgtagca aatttttatg ttgttcgcag aaataaatca caccggtata ttttaagaaa
     4621 ggagaaagtc atactatttt ttgcctattt caggaataaa cttttattaa tctattgact
     4681 ccatcaacat atcaaccaaa tatgtttaaa tttttgcatt ttttaaagct attttgaccg
     4741 cttacaagca gtcggttttt ctgaaaactt tgcaaaccta gccggcgtgg tatttggcgg
     4801 cgacctcttt taccagtaag tcgaaatcgc tttcgtaggt gagatcctgc ttgactcggt
     4861 atttttcaaa ttcgctgagg gcgaaagatt tagcaatttc agcggtaact ttgccggcat
     4921 cttgtaagat ttctcggtcg gtggcggcaa gaaagcggtt aaggcgggtt tcccagtctg
     4981 ccattgtcat cggaatttgg cgttctgcca tatcttccgc catatccaaa taggcagaga
     5041 ccagccgttg tagttgtgct aattcattat ccgataaata atttttggca accaccacat
     5101 caaaggggta aattttgcct tgcggagcgt ctttccaatt cgttagcccc atattcggct
     5161 tttcagcgtt ggctcgtgcc ataatcagct cggcggcggt atgcccgtga attgcccaat
     5221 ggagcttgtt ctgcacggtg gcaaaaaagc gtttggtggc ggttgcggtg cggtcgtagt
     5281 ccacggcggt agcgtaaatg tcggtgattt tttggtagaa cttgcgttcg gataaccgaa
     5341 tttcccgaat acgagcaagc tgttcttcaa agtatttttt gcccaaaatc gtgccgtcat
     5401 ttttcagccg ttcatcgtcc atcgcaaagc ctttaatcgc aaagctatgc acgatttcgg
     5461 ttgcccattt gcgaaactgc accgctcgct cggaattgac tttataaccc accgcaataa
     5521 ttgccgacag attataatgc ttggtgtggt agtttttgcc gtcttgggca gttatccaaa
     5581 aaaattggat aactgaattt tcagctaact cattgtcact aaagattttt ttgagatgat
     5641 agctaatggt tcgtacatca acatcataca acaccgccat cattttttgg ctaagccata
     5701 cattttcatc ggcgtaaacg gcatcaactt ggctttcgcc ggtagctgta ataaaagtga
     5761 ggtattccgc cacagaggaa cgaattaaat cttgcttaga catctctttc tcccatatag
     5821 aacactgtaa ttataaacaa tattacgaaa gctgtgtaaa tgaatagcca atttttcgag
     5881 cgagatcaca aaaaaaccgc ttgcggcata aatgctacaa gcggttgatt attactcaca
     5941 aaattgtttc caattttgtt cgctcctacg gagccgtcgg caaagccaac gttcaaaacg
     6001 caatgcgttt tgtggctaaa agttttgcaa attgtaatta catactttca gtgaaggtgc
     6061 gggtaattac gtccatttgt tgctctttgg ttaaagagtt gaaacgtacc gcatagcctg
     6121 aaacacgaat cgttaattgc ggatattttt cagggtgttc taccgcatct aataatgtat
     6181 cacggtttaa cacgttcaca tttaagtgct gaccgccttc tacagttgct tcgtggtgga
     6241 agtagccgtc cattaagcct gcaaggttac gtttttgggc ttcgtaatct ttacctaacg
     6301 catttggtac gatagagaaa gtataagaaa taccatcttt cgcataggca aacggtaatt
     6361 tcgccacaga agttaatgaa gctaccgcac ctttctggtc acgaccgtgc attgggttcg
     6421 cacccggtcc gaatggagca cctgagcgac gaccgtccgg tgtattaccg gttttcttac
     6481 catataccac gttagaagtg atagtaagca cagactgagt cggcgttgca ttgcggtatg
     6541 ttttgagttt ttgaattttc ttcataaaac gttcaactaa atcaactgca atgtcgtcca
     6601 cacggttatc gttgttaccg aattgcggat attcaccctc aatttcaaag tcggttgcta
     6661 cgtttttcgc tacaccaacc acttcgccag ctttgttttt gatttcaata tcggtacgga
     6721 ttggttttac tttcgcatat ttaatggctg aaagtgagtc tgccgctact gaaagccctg
     6781 cgataccaca cgccattgtg cggaacacat cacggtcgtg aagagccatt aatgccgctt
     6841 cgtatgcata tttatcgtgc ataaagtgga taatgttcag tgctgtcaca tattgggttg
     6901 caagccaatc cataaagctg tctaagcggg tcattacatc atcgtaatct aagtattcac
     6961 ttgtaattgc ttcagtttta ggccctactt ggtcgcctga tttctcatca acaccgccat
     7021 tgattgcgta taacaaggtt ttcgctaagt ttgcacgagc gccgaagaac tgcatcattt
     7081 tacccacgat cattggcgat acgcagcacg caatcgcata gtcatcgttt tggaaatccg
     7141 gacgcattaa gtcatcgttt tcatattgaa ctgatgaagt atcaatagac actttcgcac
     7201 agtagcgttt aaatgcttcc ggtaattgtt cagaccaaag aatagttaag tttggctccg
     7261 gtgacggccc cattgtgtaa agggtgtgta aaatacggaa gctgtttttg gttactaagg
     7321 tacgaccgtc taaacccata ccggctaagg tttcggttgc ccacattggg tcgcctgaga
     7381 ataattgatc gtattccgga gtacgcaaga aacgcaccat acgcaatttc attactaaat
     7441 ggtcgattaa ctcttgggct tcttgctcgg taattttgcc ggcttttaag tcacgttcga
     7501 tataaatatc taagaatgaa gacacacgac cgaaagacat cgccgcaccg ttttgcgatt
     7561 taaccgctgc aaggtaagcg aaatatgtcc attgcaccgc ttcttgtgca ttggttgcag
     7621 gacctgaaat atcaaaaccg tagcttgcag ccatctcttt cattttgcct aaagcacggt
     7681 gttgctcggc aatttcttca cgcagttgaa tcgtggcttg aatatcttcg ccggattcta
     7741 aacgagcttg taatgaggca aactggcgtt gtttgtcttt cattaagaag tctgcaccat
     7801 aaagtgccat acggcggtag tcaccgataa tacgaccacg accataagca tccggcaaac
     7861 cggtaattac acctgattta cggcaacgta aaatatccgg agtgtaaaca tcgaataccc
     7921 cttggttatg ggttttacgg tattcggtaa agatttgttc tacttcaggt ttaagctcac
     7981 ggcgataaac tttgcacgaa cctttcacca tattgatacc accaaacggc ataatcgcac
     8041 gttttaatgg agcatctgtt tgcagaccaa cgattttttc taacgattta tcaatataac
     8101 ccggtgcgtg tgaggtaatg gttgatggcg tgtcgcagtc aatatcatac ggttcgtgtg
     8161 ttttgttttc tactttgatc atttccatca catctgccca caatttgctg gttgcaggtg
     8221 ttacttcagc taggaaagac tcatcaccct cataaggtgt gtagtttttt tggataaaat
     8281 cacgtacatt tacttcagtt tgccactcgc ctgcggcaaa accttcccac gctttttgtt
     8341 gagcgtctgt tagttgagtc attttaagtc ctctgtgtta gtaagttaaa acgggaaagc
     8401 ggtgattttt tgcaaaaaat ttgcaaaatt tgaccgcttg aatctgaaat cttaatgctt
     8461 tctaatgtat aagaaccact gcattaaacc aatgaaaaac acgccaccga caatattacc
     8521 cagcgttgcc ggaattaagt tcttaaacac aaaatgagat acatctaaat cggcaaattg
     8581 cgttgggtca acattaattg ctgtccaaaa ttcagggctg gcaaagtgcg aaatcgcaat
     8641 ccccatcgga atcataaaca tatttgccac acagtgttca aagccggaag ccacaaacat
     8701 tgcaatcggt aacatcataa taaaggcttt atcggttagg cttttacctg cataagacat
     8761 ccacaccgca atgcacacca taatgttaca gaaaatgcct aaacagaaag cctcaaacca
     8821 ttggtggtga attttgtgct gtgcggtatt taaaataacc aaaccccatt gccctttggc
     8881 tgccataatc tgaccactaa accaaatcaa caatacaatg gaaatcgccc caacaaagtt
     8941 accgaaatac accactaccc agtttttaaa catctgtaac cagttaattc tttggctggc
     9001 tctggctaca atcgtaagtg ttgatgaggt aaaaagttct gccccaaaca ccacacacat
     9061 aatcacgccg agtgaaaata ccaaaccacc aatcagttta gccatacccc aaggcatttg
     9121 gctcgcaccc acttgggttg tggtataaaa tacaaatgct attgcaataa aaccgcccgc
     9181 agtaatggca gagagaaagg aatataattg attttttgtt gctttataaa ccgcaccatc
     9241 ttctgcgatt tttgccattt cagcaggaga aaacatcaga caaccttaac aatgagaatt
     9301 gaaaaaccga tat
//
LOCUS       DANLUQ010000099         9258 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.099, whole genome shotgun sequence.
ACCESSION   DANLUQ010000099 DANLUQ010000000
VERSION     DANLUQ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9258
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.099"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            134..922
                     /locus_tag="PXB27_001774"
     CDS             134..922
                     /locus_tag="PXB27_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346540.1"
                     /translation="MFLSHFKQVLNANIEADGKDWTIIDVFGGSGLLAHTAKREKPLA
                     RVIYNDFDNYAERLNHIKETNQLRQEIYQIVDEIIPKNKRISNEIKAKIINKINDFEG
                     FKDLKCLSSWLLFSGEQVATLDELFKHDFWHCVRQSDYPEATGYLDDIEVVSESFHQL
                     LPRFHDKEKVLLILDPPYLCTRQESYKQENYFDLIDFLRLIDLTKPPYIFFSSTKSEF
                     IRFIHYMVQNRKENWQAFEGAERIVVNASASYNGKYEDNMVYKF"
     gene            complement(959..3398)
                     /locus_tag="PXB27_001775"
                     /pseudo
     CDS             complement(959..3398)
                     /locus_tag="PXB27_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249629.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family igA-specific metalloendopeptidase"
     gene            complement(3718..5484)
                     /gene="aspS"
                     /locus_tag="PXB27_001776"
     CDS             complement(3718..5484)
                     /gene="aspS"
                     /locus_tag="PXB27_001776"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDL5346541.1"
                     /translation="MMRSHYCGALNRSHLGQTVTLSGWVHRVRNLGRFIFMQIRDREG
                     IVQVFFDEKDEALFKQASALRSEACVQIQGEVIARDESQINKEMATGEIEVLVKNLVV
                     YNNAEVLPLDFNQNNTEEQRLKYRYLDLRRPEMAEKLKTRAKITSFVRRFMDDNGFLD
                     IETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFR
                     DEDLRADRQPEFTQIDVETSFMTAEEVRALMEAMIHGLWKETLGVDLGKFPMMTWNEA
                     MTRFGSDKPDLRNPLEMVDVADILKSVDFKVFSGPANDPKGRVVALRVPNGAALTRKN
                     IDDYTQFVGIYGAKGLAWLKVNDMNTGLEGIQSPVAKFLNEEVLKALVKRLNLQKDDI
                     VFFGADNWHVATNAIGALRLKVGNDLGLTDTTAWKPLWVIDFPMFERDDEGNLSAMHH
                     PFTSPKELSPEELVQDPENAVANAYDMVINGYEVGGGSVRIFSQQMQQTVFSILGINE
                     EEQKEKFGFLLDALKFGTPPHAGLAFGLDRLTMLITGTDNIRDVIAFPKTTAAACLMT
                     EAPSYANPKALEELAIRTIPQE"
     gene            complement(5639..6550)
                     /locus_tag="PXB27_001777"
     CDS             complement(5639..6550)
                     /locus_tag="PXB27_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HDL5346542.1"
                     /translation="MLYGLAIVLIPLFLGYLITLKNHRHMAIVNRVVSLCLYLILLVM
                     GISLGQIDNILTELPQIGGTAFSLVVILLLSNIIGLVIYDKLQPMKRDKVNQDQLPSR
                     LHLLIDSFKLIGITLLGGIIGYFTKGVVDFPLHASTYVLEVMIFGVGIQLRNSGIPLR
                     EVFFNKRGIYTSIVMVASSLIGGVISAFTLDLSLAKGLTFASAFGWYSLSSVLVNDAW
                     GPIYGSIAFFNDISREILCLFLVPLFMRQFPSTAVGIGGATSLDCMLPIIQKSGGIQI
                     VPLAISFGFIVNLAAPLLLAVFIGLEG"
     gene            6673..7755
                     /gene="dinB"
                     /locus_tag="PXB27_001778"
     CDS             6673..7755
                     /gene="dinB"
                     /locus_tag="PXB27_001778"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDL5346543.1"
                     /translation="MSIQAKHKRKIIHIDMDCFYAAVEMRENPALVGKPVAVGGNANQ
                     RGVLTTCNYEARKFGLHSAMPTATALKKCPNLILLPVNMALYKSVSAQIHEIFHRYTD
                     VIEALSLDEAYLDVTECERYFGSATWIAQAIRNDIFNELHLTASAGVAPLKFLAKITS
                     DQNKPNGQCVITPDDVQNFVKNLPLAKIPGVGKVTNEKLKQLGFETCGDIQQAEKAVI
                     YRAFGKFGQRLWDFSHGIDERAVEPYRERKSLAVENTLLHDISTLEQAEKIVADLFDK
                     LVFRLQRNWGDTPLTEFKKLGIKLKFDDFSQTTLERTTDGFAYERFGELLQQIFTRRK
                     ERKVRLIGLSVHFPEKKVNKQLNLWE"
     gene            7843..8712
                     /gene="htpX"
                     /locus_tag="PXB27_001779"
     CDS             7843..8712
                     /gene="htpX"
                     /locus_tag="PXB27_001779"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HDL5346544.1"
                     /translation="MAKRVILFLLTNLAITFVLGIVLNLIFKVTGIQGQSTAGILVLS
                     LVFGFSGSLISLFMSKSMALRSVGAEVITQPRNEAEQWLFNTVQRQAQQANIPMPDIA
                     IYHSADVNAFATGATKSNSLVAVSTGLLNSMTKDEAEAVVAHEVAHIANGDMVTMTLL
                     QGVLNTFVIFLARIISTMAASSRDENGNSSTNTLVFWVVDIVLQMVFGVLATMIAMWF
                     SRQREFRADAGSAQLVGKEKMIAALQRLQRVHEPQEMEGSLAAFMINGSRGKELFMSH
                     PPLEKRIEALRNL"
     gene            complement(8766..9197)
                     /locus_tag="PXB27_001780"
     CDS             complement(8766..9197)
                     /locus_tag="PXB27_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDL5346545.1"
                     /translation="MFLSGFLSATLLPGNSEIIFTTLASQHLITYGSLSSFSLFNLLL
                     VATLGNSLGSITTYAIGLLLPRPQNTTNRYSQWALAKSEKYGVLVLLFSWLPIVGDIF
                     CGIAGWLRFSLWQSILFIVIGKLLRYLFLLATLYSVLPHIL"
BASE COUNT         2786 a         1813 c         1936 g         2723 t
ORIGIN      
        1 gcaagcaagc aagcaagcaa gcaagcaagc aagcaagcaa gcaagcaagc aagcaagcaa
       61 gcaagcaagc aagcattgtg agatagagaa agtatttaaa caagctcctt tacccttttc
      121 agggcaaaaa cggatgtttt tatcccattt caaacaggtg ttaaatgcaa atattgaagc
      181 agacgggaaa gattggacta ttatagatgt ttttggtggg agtgggttgc ttgcacatac
      241 cgcaaagcgt gagaaaccgt tagcaagagt gatttataat gattttgata attacgccga
      301 acggctaaac catatcaaag agacaaatca acttcgccaa gaaatctacc aaattgttga
      361 tgaaattata ccaaaaaata agagaattag caatgaaatt aaagctaaaa tcatcaataa
      421 aatcaatgat tttgaggggt ttaaagacct caaatgctta agttcttggt tattgtttag
      481 cggtgagcaa gttgcaactt tagatgagtt atttaagcac gatttttggc actgtgtgcg
      541 gcaaagtgac tatcctgaag caacgggtta tcttgatgat attgaggtgg ttagtgagtc
      601 ctttcatcaa ttattgccga gatttcacga caaagagaaa gtattattga ttcttgaccc
      661 accttatctt tgcacaagac aggaaagcta taagcaggaa aattactttg atttaattga
      721 tttcttacgg ttgatagacc ttactaaacc accttatatc ttctttagct caaccaaaag
      781 cgagtttatt cgctttattc actatatggt tcaaaacaga aaagaaaact ggcaagcgtt
      841 tgagggagcg gaaagaatag tggttaatgc ctcggcaagt tataacggca aatatgagga
      901 taatatggtg tataagttct aaaaatttaa acgccctttg atagtgattt aaagggcgtt
      961 aaaaaaccat taaaattaag aaagtaaagc tcctgttttc tttcgtagtt ttcgtggttg
     1021 cgtttcgcag ttttgttggc tcgctacaat tactgttcat tgtaatgttt aatcgaccat
     1081 tggttttgct aggatcagtt tcaccaaaat aaccgttaaa accttggacg gtgccttctg
     1141 ctgctttgat tttttcagca agtatttgtt gctttgcagc actttcatct gttaccttga
     1201 gtacttcaaa gtattcgttt tcacgaatga gaccagtagc aaagtctaaa tcgatgaaaa
     1261 ctttacgcgc attaaaaata gctgtgatct ctgcaacgct taaaggtttt ttcaaacgga
     1321 taaaattagt tggcaagtgt ggaaaacgtt gatatgtgaa aatatcattt gtccaaatga
     1381 cgagaaacct gatctggact agtaattgct tgaactttca cttggtcaat tgttggacta
     1441 actatgccgg ttagtgtaat gtttgctgtt tgggctgtat tatgattgac aaccatcgct
     1501 ccttcatcgc tattctgaat acggcggaac gtgagatcat tgccgttgac atctaagcgc
     1561 ccaccacgat aaccaaaata gatattgtta ggatccattt gatctgatga attgaggatt
     1621 acggtaggtc tgccactcac aatacctagc tctgaaaatg cagatttttt gttattttca
     1681 tccgcttgtt gatttaatat aacagtgccg tcaccaacgc taacggagcc tttgttttct
     1741 cctttgccgt tgatagttag tgtcccttgt ccgatttttg ataatctgtc accagcaggg
     1801 ttactgattt tccagatcac atttttatct ttatcaatat caacaccggc tcctaaccaa
     1861 gtaatgcctg ctttcatacc ttccacagtg gtattaccat gaaaatggat tgctcctgca
     1921 ccttggttga tattctctgt gagacttagc gtattgtttt cacccaaaat atggaatgtt
     1981 ttaccgtgtt ggagttgagg attccaaggg ttatcgcctg tattaccatt atgaccttga
     2041 agtgttggta atgaagtatc tgctaaatta actgtaagag gagcattccc tcctacgatt
     2101 gagctggtat tgccagaagg tgtccaagtg agtctagcac catttgcatt aatagcgcct
     2161 gcattatttt ctttttctaa agcacgtaca taatgtgggt ggtgatagtt ccagtaagat
     2221 ttataaacat ctcccccaag cgtattattc tctgagaaat agtcactata tacacctaac
     2281 acaacccatt ttttggtacg aacatcataa ccaaaaagag cggagccact atcgccaggt
     2341 aaaccgagag ttgttaaggg accgtagccg tggtctttaa atacatcgtt tatggtatta
     2401 ttttcagttt ttgctactaa aatacggctg ttaaaatcgc tgcctggaac gcttggatcg
     2461 ccattagtaa caggcagaac gctacctcct gtcaaaaaag gaccgttacc gacttcaata
     2521 cgcccgttgt ttaagcgatc tctttcgttg ttaaccactg cttgtctacc agaaccggca
     2581 cgaataaaca tggggaaaat aaaagggtcg ctaaatacac tataaaaatc ttgtacattt
     2641 tcggtagtgc gttcaatctc attagctgga gcgacttcag ttactaattt cgataaccta
     2701 ggtgcgtggt agtcccagcg gggcgttggg ttacctgaac catctaaaat cagtttttct
     2761 ccttcgtcat tacgttcacg tttttctatg cctttcccga gatctgttct ttctttattt
     2821 ggatcaggca tatagttatt acggcgcacc acactataat tgtaatgatg gtcgtcggct
     2881 ttttcgctag atgaatcacc aaattgaatg tcccgatagc cttggttgtg gtttacacta
     2941 ataacgtatt gtggattaac taatgttgca atccctgttt gacgagtaat ggaagagaag
     3001 tcaatcattg gaatagacat agtctcacct gttactgttt caaaaacagg tagatcatta
     3061 gagtcttcat cacctccacc atagagaaat tcataacaac cgggtaaact ttcattatca
     3121 aagcattcag ataggcttac attcggctta ggtgaaatat ttgattccac ttcaacctgc
     3181 ttagttggtg taacctgtaa aaaagtaccg atgtttttgc ccgttttatc aataactgga
     3241 atattggttg ccccaacaaa aaatttgcct ttattttctg ctaaatcacg aaaatattga
     3301 taatcgacat caccacgtac aatagatgcc agtaatgttg gtgaaagaga aatagcaatt
     3361 gatgttgcta gaagagagag cttgaaagta tgcttcatta ttatttgaaa cctgtataaa
     3421 tatgtaagta gatcagattt gagcgctatt aaaaagatta tttaaatcga ttaaatattc
     3481 atttaaatac cacacaatta ttcagcgcga ttatcttgtt cggcataaat ctgctcaata
     3541 cccaaataag gtaataatta aaataatagg ggatatttag tagtaacata gagaagagaa
     3601 aagagtcttt attatgttat ttagagaata attttgcgat tattactttt gcaaagtttt
     3661 tcactttttc gaccgcttgt taaataaaaa gggcgaaaaa tgtttcgccc ctaccgatta
     3721 ctcttgtgga attgtacgga ttgctaactc ttccaacgct ttcggattcg catagcttgg
     3781 tgcttctgtc attaaacacg ctgcagcggt ggttttcggg aaagcgatga catcacgaat
     3841 gttatcagtt cctgtaatca acatcgttaa gcggtctaaa ccgaaggcta agcctgcgtg
     3901 tggcggagta ccgaatttta acgcatctaa taagaaaccg aatttctctt tttgctcttc
     3961 ttcgttaatg cctaaaatgc tgaacacggt ttgttgcatc tgctggctga aaatacgtac
     4021 cgagccaccg cccacttcgt agccgttaat taccatatcg taagcgtttg ccaccgcatt
     4081 ttccggatct tgcactaatt cttccggcga taattctttc ggtgaagtga acgggtggtg
     4141 cattgccgat aagttgcctt catcatcacg ttcaaacatt gggaaatcaa tcacccataa
     4201 cggtttccac gctgtggtat cggttaagcc taaatcgttc cccactttta agcgtaacgc
     4261 accgatggcg ttagtcgcaa cgtgccagtt atccgcaccg aagaatacaa tgtcgtcttt
     4321 ttgcaagttt aagcgtttaa ctaaggcttt tagcacttct tcatttaaga attttgcaac
     4381 cgggctttga atgccttcta aacctgtgtt catatcattc acttttaacc aagccaagcc
     4441 ttttgcccca taaatgccga caaattgggt gtagtcatca atatttttac gggttagagc
     4501 tgcaccgttt ggcacacgaa gtgccactac acgccctttc ggatcattcg ccggaccgct
     4561 aaatactttg aaatcaaccg attttaaaat atctgccaca tcaaccattt ccagtgggtt
     4621 acgcaaatcc ggtttgtctg aaccgaaacg ggtcattgct tcattccacg tcatcattgg
     4681 gaatttaccg agatcaacgc ctaaggtctc tttccataag ccgtggatca tcgcttccat
     4741 taatgcacgc acttcttcgg cagtcataaa gctggtttcc acgtctattt gggtaaattc
     4801 cggctggcgg tcggcacgca aatcttcatc acggaaacat ttcacgattt ggtaataacg
     4861 gtcgaagccc gacatcatta gcaactgttt aaatagctga ggtgattgtg gcagagcata
     4921 gaatttgcct ttatgcacac ggcttggcac gaggtaatct ctcgcacctt caggagtagc
     4981 tttggtcagc atcggggttt caatgtctaa gaaaccgtta tcgtccataa aacggcggac
     5041 aaaactggtg attttagcac gggttttgag cttttctgcc atttcagggc ggcgtaaatc
     5101 caaatagcgg tattttaaac gctgttcttc ggtattgttt tgattgaagt ctaacggcaa
     5161 tacttcagcg ttgttataca ccactaaatt ttttactaac acttcaattt caccggttgc
     5221 catctcttta ttgatttgcg attcatcacg ggcaatcact tcaccttgaa tttgcacgca
     5281 cgcctcagaa cgtaaggccg atgcttgctt gaaaagtgct tcatctttct catcgaaaaa
     5341 cacctgcaca atgccttcac gatcacgaat ttgcataaaa ataaaacgac caaggttacg
     5401 aacacgatga acccaaccgc ttaaggtaac tgtttgtcca aggtgtgagc ggtttaatgc
     5461 accgcagtag tgagaacgca tcataaaatt atccttttga aaataaatac cccaagtttc
     5521 ggggaaaact taaggattat agcggaaaat tcagcgaaga tggataaata aaagcaaaca
     5581 agcggtgatt tttattccat tttttgcaaa aagtgggata aaaatcaccg cttgtgattt
     5641 atccttccag cccaataaaa acggctaata acaatggagc ggctaaatta acaataaaac
     5701 cgaagctgat tgccagcggc acaatttgaa taccgcccga tttttgaata atagggagca
     5761 tacaatctaa tgaagtcgct ccgccaatgc cgactgcggt ggacggaaat tgacgcataa
     5821 acagtggtac taaaaacagg cataaaattt cacgcgagat gtcgttaaaa aaggcaatac
     5881 tgccgtaaat cgggccccaa gcatcgttta ccaatacgct tgaaagggaa taccaaccga
     5941 atgcagaggc aaaggttaag cctttagcaa gggataaatc taaggtgaat gccgaaatca
     6001 cgccaccaat cagggaactt gccaccatta caatcgaggt ataaatcccc cgcttgttaa
     6061 agaacacttc acgcagggga ataccgctgt ttcgcagttg aatccccacc ccgaaaatca
     6121 tcacttctaa cacataggtg ctggcgtgca gagggaaatc caccacgcct ttggtgaaat
     6181 agccgatgat cccgcctaat aaggtaatgc cgattaattt aaaggaatca atcaacaaat
     6241 gcaggcggga gggaagttga tcttgattga ctttgtctct tttcatcggt tgtaatttgt
     6301 cgtaaatcac caagccgata atgtttgaaa ggagtaaaat caccactaaa ctaaacgccg
     6361 taccgccaat ctgtggcagt tcggtgagaa tgttatcaat ttgtccgaga gaaatcccca
     6421 tcactaataa aatcaagtaa aggcagaggc tgaccactct gtttactatt gccatatggc
     6481 ggtggttttt gagggttatt aagtaaccga gaaaaagtgg aatcagcaca atggctaaac
     6541 cgtagagcat tttttatcct ttttatcgct atttttaaac ttgaaattct aacaataagg
     6601 gcataaaata aaactgtttt tatatacagt tatttgtgtt gcaagcggtc gtttttgggg
     6661 aaaatattgc aaatgtcgat tcaagctaaa cataagcgaa aaattatcca tattgatatg
     6721 gattgttttt atgcagcggt cgaaatgcgg gaaaacccgg cgttggtcgg taagccggtg
     6781 gcggtgggcg gcaatgctaa tcaacgaggt gtgctgacaa cctgtaatta cgaggctcga
     6841 aaattcggct tacacagtgc aatgccaacg gcaactgcgt tgaagaaatg cccgaattta
     6901 attttattgc cggtgaatat ggcgttgtat aaatcggttt ccgctcaaat tcacgaaatt
     6961 tttcatcgct atacggatgt gattgaagcc ttgtcgcttg atgaagccta tttagatgta
     7021 accgaatgcg aacgctattt tggctccgcc acttggattg ctcaagcaat ccgcaatgat
     7081 attttcaatg aattgcattt aactgcctcg gcaggagttg ctccgcttaa atttttagca
     7141 aaaattacct ccgatcaaaa caaacctaac ggacaatgtg tgattacgcc tgatgatgtg
     7201 caaaattttg tgaaaaatct accgcttgca aaaattccgg gtgtggggaa agtaaccaat
     7261 gaaaaattaa aacagcttgg ctttgaaacc tgtggtgata ttcagcaagc agaaaaagcg
     7321 gttatttacc gagcgtttgg caaattcggg caacggcttt gggattttag ccacggtatt
     7381 gatgaacgag cggtagagcc ttatcgtgaa agaaaatcgc tggcggtgga aaatacgctg
     7441 ttgcacgata tttcaaccct tgagcaagcg gaaaagattg tggcagattt attcgacaaa
     7501 ctggtttttc ggttgcaacg aaactggggc gatacgccat taactgagtt taaaaagtta
     7561 ggcattaaac tcaagtttga tgattttagc caaaccacgc ttgaacgaac caccgatggc
     7621 tttgcgtatg agcgatttgg cgagttgcta cagcaaattt ttacccgaag aaaggaacga
     7681 aaggtgcgat taatcggtct aagtgttcac tttcccgaga aaaaagtgaa taaacagtta
     7741 aatttgtggg aataattgta aataatgact tgaaattaag gctaaagtca ttatcttatt
     7801 cgtgcaatca ttgcatctat atttttggag attgaaaaca ttatggcaaa acgtgtcatt
     7861 ctatttttat taactaactt agcgattacc tttgttttag ggatcgtatt aaaccttatt
     7921 tttaaagtga ccggcattca agggcaaagc actgccggta ttttggtgct ttcgttagtc
     7981 ttcggtttca gtgggtcttt aatttcgcta tttatgtcga aatcaatggc attgcgttcg
     8041 gttggggcag aggtgattac acagcctcgt aacgaagcgg agcaatggtt attcaacact
     8101 gtacaacgtc aggctcagca agcgaatatc ccaatgccgg acattgcgat ttaccattcg
     8161 gctgatgtaa atgcattcgc tacaggagca acgaaaagta attctttagt tgcagtcagc
     8221 accggcttgc tgaattcaat gactaaagac gaagctgaag cggtagtagc acacgaagtg
     8281 gcgcacattg cgaatggcga tatggttaca atgactttat tacaaggcgt gttaaatact
     8341 ttcgtcattt tcttagctcg tattatttcg acaatggcgg caagttcacg tgacgaaaat
     8401 ggcaattcaa gcaccaatac gctggtgttc tgggtagtag atattgtgtt acagatggtg
     8461 tttggtgtgt tagcaacaat gattgcaatg tggttctctc gccaacgtga atttcgtgca
     8521 gatgcaggct ctgctcaatt agtcggtaaa gagaaaatga ttgcggcatt acaacgcttg
     8581 caacgtgttc acgaaccgca agaaatggaa ggctctcttg ctgcctttat gattaacggt
     8641 agccgtggta aagagttgtt tatgagccac cctccgttag aaaaacgtat tgaagcgtta
     8701 agaaacttat aaagcacaag cggttgtttt tgtaaaattt tttgcaaaac agccgctttt
     8761 gttttctata aaatatgcgg taacaccgaa tagagagttg ccagcaaaaa cagataacgt
     8821 aacaatttcc caatcacaat aaagagaatg ctttgccaaa ggctaaaccg tagccagccg
     8881 gcaatgccac agaaaatatc gcctacaatc ggtaaccaac taaaaagcaa gactaacacg
     8941 ccatattttt cacttttggc taatgcccat tgtgaataac gattggtagt attctgtggg
     9001 cgagggagta atagccctat cgcataagtg gttatgctac ctaaactatt acctaaggtt
     9061 gcaaccaaga gtaaattgaa cagtgaaaat gaggataaag agccataagt gatgagatgt
     9121 tggcttgcta aggtagtgaa gataatttca gaatttccgg gtaagagggt agcgcttaga
     9181 aatccactca gaaacatagt aaagagttga ttttgttcgg aaaagaataa actgaaaaag
     9241 gaagtgagat attctgtc
//
LOCUS       DANLUQ010000100         9057 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.100, whole genome shotgun sequence.
ACCESSION   DANLUQ010000100 DANLUQ010000000
VERSION     DANLUQ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 9057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9057
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.100"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            167..883
                     /locus_tag="PXB27_001781"
     CDS             167..883
                     /locus_tag="PXB27_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL5346546.1"
                     /translation="MKKTLLTLLFGCVVTAQAQDIKFVMEPSYPPFEMTEEKGEIIGF
                     DVDIANAICKEMNANCTFHSQPFDSLIQSLKQKQFDAAISGMGITEPRKKQVLFSEPY
                     FPSSAAFIAKKDTDFAKVKTIGVQNGTTYQHYLAKEKKEYNVKSYASYQNAILDVQNG
                     RIDAIFGDVPVLAEMARKHEGLDFVGEKINNPNYFGDGLGIATHLKNQVLVDQFNAAL
                     KTIKENGEYQKIYDKWMGGK"
     gene            complement(953..1831)
                     /locus_tag="PXB27_001782"
     CDS             complement(953..1831)
                     /locus_tag="PXB27_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HDL5346547.1"
                     /translation="MKNLYKWPAAQSLYPNQGKKSYAGKRFRYRLRSWLHHSKIKQFE
                     QFVTQNPQLIPLLNARPNYSYPVAHRFLDKRFSAKQRLQKITDNLLFLPQKLAHLPPL
                     WEQAVNFGEIIADFELWLNINEHQPMEGFWALELRHRPTNQLVYLLTFGKLDEALLIA
                     VIQGPNFEGSKELVKQLTKSCHGLRPAYLMVETMKALTAVLGYQSLLGIPQKYQNKSR
                     WVQSKRYVVDYDAIFAESGGKPGNYWQLPLELESKKIDEIPSKKRSMYRKRYEMLANL
                     KQTMELMLKTYTGLNG"
     gene            complement(1907..3232)
                     /gene="hslU"
                     /locus_tag="PXB27_001783"
     CDS             complement(1907..3232)
                     /gene="hslU"
                     /locus_tag="PXB27_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HDL5346548.1"
                     /translation="MMSMTPREIVSELDAHIIGQKDAKRAVAIALRNRWRRMQLPEDL
                     RQEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LADVSMKLVRQQAVEKNKMRAQDAAEDRILDVLLPPAKDQWGNVQESDNNSTRQTFRK
                     KLREGQLDDKEIEIDVAAQVSVEIMTPPGMEEMTSQLQSLFEGMSPSKTKKRKMKIKD
                     ALKVMLDEEAAKLVNPEELKQQAIEAVEQHGIVFIDEIDKICKKGEHSGGDVSREGVQ
                     RDLLPIIEGSTVNTKHGMVKTDHILFICSGAFQVARPSDLLPELQGRLPIRVELKSLT
                     KEDFERILTEPNASLTLQYRELMKTEGVEIEFTKDGISRIAESAFRVNEKTENIGARR
                     LHTVLERLMDGISFDASERRGEKIIIDENYVSEALNDVVENEDLSRFIL"
     gene            complement(3489..4010)
                     /gene="hslV"
                     /locus_tag="PXB27_001784"
     CDS             complement(3489..4010)
                     /gene="hslV"
                     /locus_tag="PXB27_001784"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HDL5346549.1"
                     /translation="MTTIVCVRKDGKVAIGGDGQATLGNCIEKGTVRKVRRLYKDKVV
                     TGFAGSTADAFILRDLFEKKLELHQGHLVKSAVELAKEWRTERALRRLEAMMIVANDS
                     EFLLVSGSGDVIEPEQDVLAIGSGGNYAKAAALALLRTENNLSAKEIVAEALKIAGDI
                     DIYSNYNHVIEEV"
     gene            complement(4071..4784)
                     /gene="sfsA"
                     /locus_tag="PXB27_001785"
     CDS             complement(4071..4784)
                     /gene="sfsA"
                     /locus_tag="PXB27_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HDL5346550.1"
                     /translation="MKFPPLSHGILLRRYKRFLADIQLPNGEQITLHCPNTGAMTGCA
                     NPGDTVWFSTSHNPKRKYAHTWELTQTQAGDFICVNTLRANQLVQEALENQWISELAD
                     YQHILPEQKYGSENSRIDFLLKSDLLPDCFVEVKSTTLLTENGVGMFPDAKTERGQKH
                     LRELTKIAEQGLQAVIFFAILHTGIERFEVAKQIDPKYAELFAQAIAQKVQVLSYKMA
                     ISLENGQPIAATLTKKCEF"
     gene            complement(4800..5669)
                     /locus_tag="PXB27_001786"
     CDS             complement(4800..5669)
                     /locus_tag="PXB27_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDL5346551.1"
                     /translation="MLYLLIATLIWASSFIVGKVAISAFDPVQIMQIRLTLAAFIALP
                     FFIPAYKKIQKNQYIKVWLISFLTFPLYVILQFSGLKLTSAASAVALLGLNPLLTVLV
                     GYFCFKKQATRLDFILSLFACLGILIMASGSDDSGSINVLGLLLVTLGSLSFIVGMYL
                     SKEILQSVKVIDLTKIMLVQGAITALPITLLFAENWQLPTSLSAWASVAYLGIICTSL
                     AILLWNKGISQSSPILAGILLALEPVFGLLMALVFLSEQLSLVTWVGIIIVICTALLS
                     VLLPMMKRYKRLI"
     gene            complement(5670..6062)
                     /locus_tag="PXB27_001787"
     CDS             complement(5670..6062)
                     /locus_tag="PXB27_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL5346552.1"
                     /translation="MKSPILGFHHIALIVSDYEKSKHFYTRILGAEIIAETYREQRAS
                     YKLDLRFKDGSQIELFSFPETPPRFTSPETCGLRHLAFRVENIEYAIAFLEQHQLPHE
                     GIRIDEITGKRFTFFRDPDDLPLEFYEV"
     gene            6146..7291
                     /gene="mutY"
                     /locus_tag="PXB27_001788"
     CDS             6146..7291
                     /gene="mutY"
                     /locus_tag="PXB27_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDL5346553.1"
                     /translation="MNSNSPFPLAKAKASDPFAKAVLAWFEQYGRKHLPWQQNKTLYQ
                     VWLSEVMLQQTQVATVIPYFARFMERFPTVIDLANASIDEVLHLWTGLGYYARARNLH
                     KAAIQIRDEFGGEFPTRFDDVLALTGVGRSTAGAILSSVLDAPHPILDGNVKRVLSRY
                     FAVEGWAGEKAVENRLWQLSESVTPDTQVADFNQAMMDLGAMICTRTKPKCLLCPLQE
                     NCKTNAMQAWDSFPAKKPKKTLPERQTYFLVLKSGSKVLLQKREAKGIWGGLFVFPQF
                     ESLEALKRSDFMQNLQISQQLTAFRHTFSHFHLDITPVLVETDLKKTSENSPLVVEEK
                     AGNYLSKVSSNADYWYDLHQHNEVGLATPVKRILDELAFSVKNEIKD"
     gene            7295..7564
                     /locus_tag="PXB27_001789"
     CDS             7295..7564
                     /locus_tag="PXB27_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HDL5346554.1"
                     /translation="MARTVFCEYLKQEAEGLDFQLYPGELGKRIFDSISKQAWSEWIK
                     KQTMIVNEKKLSMMNPEHRKLLEQEMVNFLFEGKEIIIEGYKPVE"
     gene            7581..8672
                     /gene="mltC"
                     /locus_tag="PXB27_001790"
     CDS             7581..8672
                     /gene="mltC"
                     /locus_tag="PXB27_001790"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HDL5346555.1"
                     /translation="MKKYAKYLPMLAIIPFLIACGSNKSTKKPKSTRVDYSKDTNGLD
                     ILAGQFSHNIDDIWGSNELLVASKKDYVKYTDKFYTRSHISFEEGVITIETLGNQNHL
                     RNSIIHTLLMGRDPKGIDLFASGDAPISSNPFLNGQVEDQFGRNITNVAIANDFATYL
                     LQNKVQTRRLKNGQTVSFVTIKMVAGHIEVRARQYLPLVRKMAKQYGIEPSLILGIME
                     VESAFNPYAISYANAIGLMQVVPRTAGRDIFARKGMGGQPSREYLYNPSNNIDAGTLY
                     LTILRDEYLDGIEDPVAKRYAMISAYNSGAGAVLRVFDSDRYSAIDRINDLSSDAVYR
                     ILTTAHPSSQARNYLRKVTTAREKYRNIR"
     gene            8804..8879
                     /locus_tag="PXB27_001791"
     tRNA            8804..8879
                     /locus_tag="PXB27_001791"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8837..8839,aa:Phe,seq:gaa)
     gene            8885..8960
                     /locus_tag="PXB27_001792"
     tRNA            8885..8960
                     /locus_tag="PXB27_001792"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8918..8920,aa:Asn,seq:gtt)
BASE COUNT         2624 a         1828 c         1922 g         2683 t
ORIGIN      
        1 ttttgggttg tttttttgag atagaggaat caagaggatt gagaaactac atctcaattt
       61 gatgtgtttg tatgacataa aacaaacttt atgaaaagaa gacttttttt aagaaagtaa
      121 ctgcgttaaa gtggtaatcc ttataatgaa aacacaggga ctttttatga aaaaaacact
      181 tttgaccctt ttattcgggt gtgtggttac tgcgcaggca caagatatca aatttgtgat
      241 ggagccaagc tatcctccat ttgaaatgac cgaagagaag ggagaaatta tcggttttga
      301 tgtggatatt gcaaatgcta tctgtaaaga gatgaatgca aattgtacat tccatagcca
      361 accttttgat agcttaatcc aaagcctaaa acaaaaacaa tttgatgctg caatttcagg
      421 tatgggtatt acggaaccac gtaagaaaca agtattattt agcgagcctt actttccaag
      481 ctctgctgcc ttcattgcga aaaaagatac agattttgct aaggtgaaaa cgattggtgt
      541 gcaaaatggg actacttacc aacactattt agcaaaagag aaaaaagagt acaatgtgaa
      601 atcttatgcc tcttatcaaa atgctatttt agatgtgcaa aatgggcgaa ttgatgctat
      661 ttttggcgat gtgccggtat tggctgagat ggcaagaaaa cacgaagggc tagattttgt
      721 aggggagaaa atcaataatc caaactattt tggtgatggt ttaggcattg caactcactt
      781 gaaaaaccaa gtattagttg atcaattcaa tgcagcatta aaaacgatta aagagaatgg
      841 cgaatatcaa aaaatctatg ataaatggat gggcggcaag taacttgtaa gcggtcattt
      901 tttgcaaagt tttgcaaagt tttgcagatt ttgaccgctt gtattctttg atttacccat
      961 ttaagccagt ataagtttta agcataagct ccatggtctg ctttaaatta gctagcattt
     1021 cataacgctt gcgatacatt gagcgttttt tgcttggaat ttcatctatt tttttacttt
     1081 ccaattcaag gggtaactgc caataatttc ccggctttcc gcccgattcc gcaaaaatag
     1141 catcataatc cactacatag cgcttacttt gcacccagcg ggatttattc tggtatttct
     1201 gtggaatgcc tagtaaactc tgatagccaa gaacagccgt taaagccttc atcgtttcaa
     1261 ccattaaata ggccgggcgt aagccgtggc aacttttggt taattgtttc accagctctt
     1321 ttgaaccttc aaaattcgga ccttgaatta cggctatcaa caaggcttca tctaacttac
     1381 caaatgttaa taaataaact agctgattag tcggtctgtg gcgaagttct aatgcccaaa
     1441 aaccttccat cggctgatgt tcattgatgt taagccataa ctcaaaatct gcaataattt
     1501 caccaaaatt gaccgcttgt tcccatagag gtggtaaatg agccaatttt tgcggtaaaa
     1561 acagcaggtt atctgtgatt ttttgtaagc gttgttttgc actgaaacgc ttatctaaaa
     1621 agcggtgagc cacggggtag ctgtaatttg gtcgagcatt taagagcgga atcagttgtg
     1681 gattttgtgt aacgaactgc tcaaattgct tgattttgga atgatgcaac caagagcgaa
     1741 gacggtaacg aaaacgtttc cctgcgtaag acttctttcc ctgatttgga taaagcgatt
     1801 gtgcagcagg ccatttgtat agatttttca tttttacaac taacaatatg aaaagcggct
     1861 aattcgcaag atttttcgct aaaataaccg cttgtataac ttaaaactac aaaataaagc
     1921 gacttaaatc ttcgttttcc accacgtcat ttaacgcttc ggaaacatag ttttcatcaa
     1981 tgatgatttt ttcaccacgg cgttcgctgg catcgaacga aatgccgtcc atcaaacgct
     2041 caagcacggt gtgtaaacgt cttgccccga tgttttcggt tttctcgttc acacggaatg
     2101 cactttctgc aatacggctg ataccgtctt tagtgaactc aatctccacg ccttcagttt
     2161 tcatcaactc acgatattgc aaggttaatg aggcattcgg ttcggttaaa atacgctcaa
     2221 aatcttcttt ggtaagcgat tttaactcca cacgaatcgg caagcgaccc tgtaattctg
     2281 ggagtaaatc agacgggcga gccacttgga atgcccccga gcagataaac agaatatggt
     2341 cggttttcac cataccatgc ttggtattta ccgttgagcc ttcaatgatt ggaagtaaat
     2401 cacgctgaac accttcacga gaaacatctc cacccgaatg ctcgcctttt ttacagattt
     2461 tgtcgatctc atcaataaag acaataccgt gctgttccac cgcttcaatc gcttgctgtt
     2521 tcagctcttc aggatttact aatttcgccg cttcttcatc aagcatcacc tttaacgcat
     2581 ctttgatttt cattttgcgt tttttggttt tgcttggcga cattccctca aacagcgatt
     2641 gtaattgcga ggtcatctct tccattcccg gcggggtcat aatctcaacg ctcacttgag
     2701 ctgccacgtc aatttcaatc tctttatcgt ctaattgccc ttcacgcaat tttttgcgga
     2761 aagtttggcg ggtagaatta ttgtcggatt cctgcacatt tccccactga tctttcgccg
     2821 gtggcaataa tacgtctaaa atgcgatctt ccgctgcatc ttgtgcacgc attttatttt
     2881 tttcgaccgc ttgttggcgg actaatttca tcgacacatc agctagatca cggataatcg
     2941 aatccacttc tttacccaca taccccactt cagtgaattt ggttgcctca actttgataa
     3001 aaggggcatt ggcaagcttc gccaaacggc gggcgatttc ggttttaccc acaccggtcg
     3061 gaccgatcat taaaatattt ttgggggtca cttcttggcg gagatcttcc ggcaactgca
     3121 ttcttctcca gcggttacgc aaagcaattg ctaccgcacg ttttgcatct ttttgtccga
     3181 taatgtgtgc gtctaattct gagacgattt cacggggtgt cattgacata atgttattcc
     3241 ttaaattaat taccctctcc ccttgtggga gagggacaga tttttctttg ttcagaagaa
     3301 aaatcaggga gagggtaaca agcggtcttt tttcttaaac gttttgcatt aacccctctc
     3361 tctgaaaaat atttctgaac gaaatatttt tcggtctcaa tactcgctac gctcgctcct
     3421 gtggagccgt tggcaaagcc aacgttcaaa tgtaaacatt tgtcccacaa ggggagagag
     3481 ttatttaact aaacttcttc aatcacgtga ttgtagttac tgtaaatatc aatatcgccg
     3541 gcgattttta acgcttccgc aacgatttct tttgcagata aattattttc ggtgcgtaat
     3601 aacgcaagag cagcggcttt cgcatagtta ccgcctgaac caatcgccag cacatcttgc
     3661 tccggctcaa tcacatcgcc cgaacctgaa accaataaaa actcgctgtc attggcgaca
     3721 atcatcatcg cttctaaacg gcgtaaagca cgctcggtac gccactcttt tgccagttcc
     3781 actgccgatt tcactaaatg cccttggtgt aactccaatt ttttctcgaa taaatcacgc
     3841 aaaataaagg catcagcggt cgagccggca aagcctgtca ccactttgtc tttatacaaa
     3901 cggcgaactt tccgcaccgt gcctttctcg atacaatttc caagcgttgc ctgaccatca
     3961 ccaccgatag ccacttttcc gtctttacgc acgcatacaa tcgttgtcat actaaatcct
     4021 tctttttaaa aactttggct tatatgggga tatgctgtgt gttttcaagc ttaaaactca
     4081 catttctttg ttaaggttgc cgcaataggt tgcccgtttt ccaatgaaat tgccatttta
     4141 taggagagaa cctgcacttt ttgtgcaatg gcttgagcga ataattcggc atatttgggg
     4201 tctatctgct ttgccacttc aaaccgttca atacctgtgt gcaaaatagc gaaaaaaatg
     4261 accgcttgta agccctgttc agcgattttc gtcagctccc gcaaatgttt ttgcccccgc
     4321 tcggttttgg catcggggaa cattcccaca ccgttttcgg ttaaaagggt ggtagatttc
     4381 acttccacaa aacaatccgg cagaaggtcc gatttgagca aaaaatcaat ccgactgttt
     4441 tcactgccat atttttgttc aggcagaatg tgctgataat ccgcaagctc gctaatccat
     4501 tgattttcca aggcttcttg tacaagctga ttggctctca aggtattgac acaaataaaa
     4561 tcccccgctt gggtttgggt tagttcccaa gtgtgagcat atttacgttt ggggttatga
     4621 gaggtggaaa accaaactgt atcaccgggg ttggcacagc ctgtcatcgc accggtattt
     4681 gggcaatgga gggtaatttg ttcgccattt ggcaactgaa tatcggcaag aaaacgttta
     4741 taacggcgaa gtaaaatgcc gtgggaaaga gggggaaatt tcatttgcaa aaaatcctgt
     4801 taaattaacc gcttgtatct tttcatcatc ggtagcaaaa cggagagtaa ggcggtgcaa
     4861 ataacaataa taatcccaac ccaagtcact aaactgagct gttctgataa aaagaccaat
     4921 gccattagca gtccgaatac cggttcaagt gcgagtagaa tacctgcaag aataggcgaa
     4981 gattggctga tgcctttgtt ccataacaat attgccaagc tggtacaaat aatgcctaaa
     5041 tatgctacgc tcgcccatgc tgagagactg gttggcagtt gccaattttc tgcaaagagt
     5101 aaggtaatag gtaaggcggt aattgcccct tgcaccagca tgatcttggt taaatcgatc
     5161 acttttactg attgcagaat ctctttgctg aggtacattc ccacaataaa acttaaactg
     5221 cctaaggtta caagcaataa tcctaataca ttgattgaac cgctatcatc gctacccgat
     5281 gccataatta aaatccccaa gcaggcaaat aaacttagga taaaatcgag cctggttgcc
     5341 tgttttttga aacaaaaata gccgactaac acagttaaaa gcgggtttag cccgagtagt
     5401 gccacggcac ttgctgcgga agttaatttc aagccggaaa attgtaagat cacgtacaga
     5461 ggaaaagtga gaaaactaat cagccagact ttaatgtact gatttttctg gattttttta
     5521 tacgcaggaa taaaaaacgg cagagcgata aacgccgcta acgtcagccg aatttgcata
     5581 atttggacag ggtcaaaggc tgaaatcgcc actttaccta caatgaatga actcgcccaa
     5641 atcagggttg caatcagtaa atataacatt tagacctcgt aaaactctaa cggcagatcg
     5701 tccggatctc ggaaaaacgt gaagcgtttg ccggtgatct catcaattcg gatcccttca
     5761 tgcggtaatt gatgttgttc taaaaatgcg atggcatatt caatattttc cactcgaaat
     5821 gccaaatggc gaagcccgca ggtttcgggc gaggtgaaac gaggcggcgt ttcaggaaaa
     5881 ctgaataatt caatttgtga gccatcttta aagcgtaaat ctaatttata gctggcacgt
     5941 tgctctcggt aggtttctgc gatgatctct gcccctaaaa tgcgggtgta gaaatgtttg
     6001 gatttttcgt aatcggaaac aattaatgca atgtggtgga aacctaaaat gggcgatttc
     6061 atcttttttc cttaatgtgt aaaatttttg gtaaaactga ccgcttgcag tatgataatt
     6121 tatttttcgg atttcaagat ttttaatgaa cagcaattcc ccatttcctt tagcgaaagc
     6181 aaaagcttct gatccgtttg ccaaagctgt tttagcttgg tttgagcaat atgggcggaa
     6241 acatttacct tggcagcaaa ataaaacgct gtatcaggtt tggttgtcgg aggtaatgtt
     6301 gcaacaaact caagtagcga ccgttattcc ttattttgca cgttttatgg aacgatttcc
     6361 caccgtaatt gacttagcta atgcctcgat tgatgaagta ttgcatttgt ggacagggct
     6421 gggttattac gctcgggcaa gaaatttgca taaagcggca attcaaattc gagatgagtt
     6481 tggcggtgaa tttcctaccc gatttgatga tgtgttggca ttaaccggag tgggcagaag
     6541 tactgcaggg gcgattttgt cttctgtgtt agatgcccca cacccgattt tagatggcaa
     6601 tgtcaaacgg gtgttaagcc gctattttgc agtagaaggc tgggcgggtg aaaaagcggt
     6661 ggaaaatcgc ctttggcaat taagcgaaag tgtgacgcct gatacccaag tggcagactt
     6721 taaccaagcg atgatggatt taggggcgat gatttgcacc cgtacaaagc ctaagtgctt
     6781 gctctgcccg ctacaagaaa attgtaaaac gaatgcaatg caagcgtggg atagctttcc
     6841 tgccaagaaa ccgaaaaaaa ccttgccgga gcgtcaaacc tattttttag tgctgaaaag
     6901 cggctcgaaa gtgctgttac aaaaacggga agcaaaaggc atttgggggg gcttatttgt
     6961 gtttccgcaa tttgagagct tagaggctct caagcggtct gattttatgc aaaatttgca
     7021 aatttcacag caattaactg catttaggca tacttttagc cattttcatt tggatattac
     7081 cccggtttta gtagaaaccg atttgaaaaa aacaagtgaa aattcaccgc ttgttgtcga
     7141 agaaaaagca ggaaattatc tttctaaggt atcttcaaat gcggattatt ggtatgattt
     7201 gcaccaacat aacgaagtgg gtttagcaac gccggttaaa cgtattttag atgagctggc
     7261 attcagtgtg aaaaatgaga ttaaggatta aattatggct cgtaccgtat tttgcgaata
     7321 tttaaaacaa gaggcagaag gtttagattt tcaactttat ccgggggagc tgggtaagcg
     7381 tatttttgac tcaatcagca agcaagcgtg gtctgagtgg attaaaaaac agacaatgat
     7441 cgtcaatgag aaaaaactca gtatgatgaa ccccgaacac cgtaaactgc tcgaacaaga
     7501 gatggtcaat tttttatttg aaggtaaaga gatcatcatc gaaggctata agccggtcga
     7561 ataataaagg ttaaatcaca atgaaaaaat atgctaaata tttgccgatg ttggcaatta
     7621 ttccgtttct tattgcttgt gggagcaata aatcgactaa gaagccaaaa tctacccgag
     7681 tagattattc taaagatacc aatggtttgg atattttggc agggcagttt tctcacaata
     7741 ttgatgatat ttggggaagt aacgagctgt tagttgccag taagaaagat tatgtgaaat
     7801 atacggataa gttttacacc cgtagccata tttcgtttga agaaggggtg attacgattg
     7861 agactttagg gaatcagaat catctacgca attcgattat tcatacttta ttaatgggca
     7921 gagacccgaa agggattgat ttatttgcct caggcgatgc accgattagc tctaaccctt
     7981 tcctaaacgg acaggttgaa gatcaattcg ggcgaaatat taccaatgtt gccatcgcca
     8041 atgattttgc gacttatttg ttgcaaaata aggtgcaaac acgccgtttg aaaaatggtc
     8101 aaactgtgag ctttgtaacg attaaaatgg tggcaggaca cattgaagta cgtgccagac
     8161 aatatttacc gctggtgcgt aaaatggcga agcagtatgg tattgagcca agtttgattt
     8221 tagggattat ggaagtcgaa tcagcattca acccttatgc catcagctat gccaatgcga
     8281 ttggcttaat gcaggttgta cctcgtaccg ccgggcgtga tatttttgcc cgtaaaggta
     8341 tgggagggca gccaagtcgt gaatatcttt ataatccatc aaataacatt gatgcaggta
     8401 cgctttattt gactattttg cgtgatgaat acttagatgg aattgaagat cctgtggcta
     8461 aacgctatgc aatgatttct gcatacaata gcggagcagg tgcggtatta cgagtgtttg
     8521 attcagaccg atactctgcg attgatagaa ttaacgatct ttcgtctgat gcggtttatc
     8581 gaattttaac cactgctcat ccatcttctc aagctagaaa ttatttaaga aaagtaacca
     8641 ccgccagaga gaaatatcgc aatattcgtt aatgtgtgaa ttatgctgtt ttttagccga
     8701 aaagtataaa aaaagcacat ttgagcagcg gtttttttaa atttgcaaaa aaacgtttga
     8761 cgttgcaagc tgaaatcagc ataatgcacc acgcaacgac gttgcctcga tagctcagtc
     8821 ggtagagcag gggattgaaa atccccgtgt cggtggttcg attccgcctc gaggcaccat
     8881 caattcctcc ttagttcagt cggtagaacg gtggactgtt aatccatatg tcgcaggttc
     8941 gagtcccgca ggaggagcca tattctgaag ccctgatgag taatcatcag ggttttcttt
     9001 ttgagctttc cggctaacta ctaagaaatt gctcaatttt tagtattatg cgttcaa
//
LOCUS       DANLUQ010000101         8992 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.101, whole genome shotgun sequence.
ACCESSION   DANLUQ010000101 DANLUQ010000000
VERSION     DANLUQ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8992
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.101"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            344..1348
                     /locus_tag="PXB27_001793"
     CDS             344..1348
                     /locus_tag="PXB27_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL5346556.1"
                     /translation="MPGDANKIISNGTSAGGALSTLLGASADHYDYEPYLKEAGALNA
                     SDKIFAVSAYCPITNLENADMAYEWQFNGVNEYSRIDMSRLNAAEFNDRSKPKPKPKI
                     EGSLNEAEIKVSNELAERFPTYLNSLHLVDEKGNPLTLDPKGNGSFKDYLSEVVKTAA
                     NKAYRGLVQDSEEQKAFQQISWLSFEKGKVSSVDWFGYVFSDKRMKSPPAFDALNGSS
                     GENNLFGTDTENNRHFTLYSAERSANKDLNLADPQIVKRMNPMHYLDNPNAAEHWRIR
                     VGTADRDTSLAISAILAIKLQMAGKNVNYETPWNVPHSGDYDVNELFLWADELVKGKK
                     "
     gene            1403..3253
                     /locus_tag="PXB27_001794"
     CDS             1403..3253
                     /locus_tag="PXB27_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL5346557.1"
                     /translation="MQVKKFSQKYINWVLKLGRLKASLLGFFMVALSAILIQSSLTYL
                     FIGRVVVVDILRSIAFGLISAPFVLYFFTLLVEKLEISRLKLEKAVRDLEELREQDAY
                     LNAKLEKNSRDKSILMATISHELRTPLNGIIGLSQILLDDKTTEKQQEYLKTINMSAT
                     ALAHIFGDIIDLEKIDSSRIELVRKEVEFEQLIHDIANFGHFMAGNKQINFEVKYPPD
                     LPKYIYIDHARLSQILWNLISNAVKFTPASGRILLEIYQLDKEQFSFAVTDSGIGIPE
                     KDQRKIFDMFYQAENSKEKKAQGSGIGLSVSKRIAKLMGGDLVVASQENQGSTFTLTV
                     QAESTETKPIFAIQQHSLKVLLVEDIEMNVVVARAMLEKIGCVVDVAMSGEETFQHFA
                     QNSYDLILLDIQLPDTTGFEIAQQLRLQYENEEIDYLPLLVALTANVIQSRQEYQAQG
                     MDDVLHKPLSLDELMRCLNQHFGDDFLQKNEEKQPLVERGSENLPYSITILNELIGVM
                     GKENVLANMDLFADLMPTYLANLQRYLSDWQTTNSVESRKLTADEAHKIKGALASVGL
                     HQLQQTAQLAQSDNGEQWEQNIAHWVEMICSKWQMDLQLAKEYVRSKGDI"
     gene            complement(3349..3903)
                     /locus_tag="PXB27_001795"
     CDS             complement(3349..3903)
                     /locus_tag="PXB27_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDL5346558.1"
                     /translation="MKSQIYYDYYPSPIGQLLLLATEEGLIYIELGQEQQTTSLDNFL
                     SAQAASGQILEIFCKTKEILDRYFAGEPLDFQQLDFLAPQGTPFQQSVWKILREIPYG
                     QTTTYGEIATQLGKPSAMRAVGGAVGRNPISILVPCHRVLGKNQALTGFGGGLPNKRF
                     LLQLEDITYKDKGIEFVKPKTKKW"
     gene            complement(3970..5451)
                     /locus_tag="PXB27_001796"
     CDS             complement(3970..5451)
                     /locus_tag="PXB27_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HDL5346559.1"
                     /translation="MKMKNFSKTLIASLVAFSSAGASAAAFQLAEISTSGLGRAYAGN
                     AAVADNASVVATNPALMTLFKQTEISAGGIFVDADVNIDGKLNATGADASHKNVIPTA
                     IVPNAYIVSPINERLSIGGGVNVNYGLISEFDPNYSAGFLGGRTSLSAVNVNFSAAYD
                     LGYGFVLGAGLNAVHAKAELGRFLGAGAARFAPAKQFLEDKKVQVQSAIQGLKALEAG
                     IAAGNTALEAQKTQVLAQVNGLLKSQNLNQTITNSAQLERLVNGIQNTNASSTIHKLK
                     GDKWALGWNVGLVYNINERNRLGFTYHSEIDLKFKGKYSNTVPVVIPKMVDTITGGAE
                     IDGTLRLRLPAFWEVSGYHKLTDKLAAQYSYKRTDWSKFQSLEAYDMQGNRLLHKTEN
                     FNDSQRIALGLSYDVNEMLTLRTGVAFDESASVTHPSISIPDTDRAWYSVGATVRFTP
                     NLSADVGYSHLRGSNLTFSEDGLATFKSKARANLYGLNVNYKF"
     gene            5799..6251
                     /gene="iscR"
                     /locus_tag="PXB27_001797"
     CDS             5799..6251
                     /gene="iscR"
                     /locus_tag="PXB27_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HDL5346560.1"
                     /translation="MKLTSKGRYAVTAILDIALHGKLEPVSLADISDRQAISLSYLEQ
                     LFAQLRRDGIVQSVRGPGGGYQLGKKPEEISVGMIISAVNENIDVTKCKGSGNCRKDS
                     QCLTHSLWKRLEDQIEQFLGTISVADLAAEHADHDCEKEHCHDHPHHH"
     gene            6306..7526
                     /locus_tag="PXB27_001798"
     CDS             6306..7526
                     /locus_tag="PXB27_001798"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HDL5346561.1"
                     /translation="MKLPIYLDYAATTPMDERVAKKMMEYMTKDGIFGNPASRSHKFG
                     WEAEEAVDVARNQIADLIGADSREIVFTSGATESDNLAIKGAAHFYQTKGKHIITVKT
                     EHKAVLDTCRQLEREGFEVTYLDPEEDGILDLAKLKAAIRPDTILVSVMHVNNEMGVI
                     QPIKEIGAICRAKKIIFHVDATQSVGKIEVNVQDLNVDLMSFSSHKLYGPKGIGGLYV
                     CRKPRVRLEAIIHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMARLTVLRDR
                     LYNGFKDIEEVYVNGSMEEGKRIGSNLNISFNFVEGESLMMSLRDIAVSSGSACTSAS
                     LEPSYVLRAIGRDDELAHSSIRFTLGRWTTEEEIDHTIEIVKKAIVKLRELSPLWEMF
                     KEGVDLSKIEWNHH"
     gene            7653..8036
                     /gene="iscU"
                     /locus_tag="PXB27_001799"
     CDS             7653..8036
                     /gene="iscU"
                     /locus_tag="PXB27_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HDL5346562.1"
                     /translation="MAYSEKVIDHYENPRNVGTFDKEASDTGTGMVGAPACGDILRLQ
                     IKVNDQGIIEDARFKAYGCGSAIASSSLITEWVKGKSLEEAGAIKNSDIAEELELPPV
                     KVHCSILAEDAIKAAIADYKEKQAK"
     gene            8145..8468
                     /gene="iscA"
                     /locus_tag="PXB27_001800"
     CDS             8145..8468
                     /gene="iscA"
                     /locus_tag="PXB27_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HDL5346563.1"
                     /translation="MSITLTEAAANRVRTFLENRGKGIGLRLGVKTSGCSGLAYVLEF
                     VDVLNEDDQVFEQHGVKVIVDEKSLTYLNGTQLDFVKEGLNEGFKYTNPNVKNECGCG
                     ESFSV"
     gene            8478..>8992
                     /gene="hscB"
                     /locus_tag="PXB27_001801"
     CDS             8478..>8992
                     /gene="hscB"
                     /locus_tag="PXB27_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HDL5346564.1"
                     /translation="MNNPFTLFDLPVQFQLDNAQLSERYLALQKQLHPDNFAHKSSAE
                     QLEAVQKSADVNEALNILKDPILRAEAMIEIHTGEAKNLEEKSMRDVDFLMQQLEWHE
                     KLETIEHRKDETELTVFLKQIKAEQKAVLEQLEQTLNTADWQKANALTDKLRYFKKLI
                     IQIEKVEEKFF"
BASE COUNT         2871 a         1599 c         1924 g         2598 t
ORIGIN      
        1 gacttcatct tttgataaac tctttgcgat ttaaacagaa aatatattta gaatgtaaac
       61 aaacatgagt cgattttatg ttcaaagagc tgtaataaga cggaataatg tcagaccaag
      121 atagatactt tcaagtaaaa tcaagcataa aaatgatttt cagactttac aaatctgaaa
      181 gaatgtgtaa gatcaggatt gtccgaaatt cggatggaaa atcccagtgg atgtattggc
      241 tggatacatc ataataacta tgtatttgtg aatgttattt gaatgattct acgtagttga
      301 ttcaaaatca gcagtgcggt atctgcatgc taatgatgaa aaaatgccgg gcgatgcaaa
      361 taaaattatc tctaacggca ccagtgccgg aggagcatta tctactttgt taggagcttc
      421 agctgatcat tacgactatg agccttattt gaaagaggcg ggggcattga atgccagtga
      481 caaaattttt gcagtttctg cctattgccc aattaccaac cttgaaaatg cggatatggc
      541 gtatgaatgg caatttaatg gtgtcaatga atatagccga atcgatatga gtcgattaaa
      601 tgcagcagag tttaacgacc gctcaaaacc aaaaccaaag ccgaaaattg aaggctcgtt
      661 aaatgaggcg gaaattaaag tttctaatga attagcagaa cgctttccga cttatttgaa
      721 cagtttgcat ttggttgatg agaaaggcaa tcctttaaca cttgatccaa aaggcaacgg
      781 ctcgtttaaa gattatttat ctgaagtggt aaaaacggct gcaaataaag cctatagagg
      841 tttagttcaa gatagcgaag agcaaaaagc ctttcaacaa attagttggc tttcatttga
      901 gaaaggcaaa gtcagcagtg tggactggtt tggttatgtg ttttcagata aacggatgaa
      961 atcaccacct gcatttgatg cgttaaatgg aagttcgggc gagaataatt tatttggcac
     1021 agacacggaa aataaccgcc attttacgct ttatagtgcg gaacgttctg ccaataaaga
     1081 tctcaattta gccgatccgc aaatcgtgaa acggatgaac ccgatgcact atttggataa
     1141 cccaaatgct gcggaacatt ggcggattag ggtgggaacg gcagatagag atacctcgct
     1201 ggcaatttct gcaatcttag caataaaact gcaaatggca ggcaaaaatg ttaattatga
     1261 aaccccgtgg aatgtacccc attcaggtga ttatgatgta aatgaattat tcctatgggc
     1321 tgatgagcta gtcaaaggca aaaagtaaaa aatagagtaa aataaagcag atctgatggt
     1381 ctgctttttg ttttgaggtc gtatgcaagt taaaaaattt agccaaaaat atattaattg
     1441 ggtattaaag ctagggcggt taaaagcttc tcttttaggc ttttttatgg tcgctctttc
     1501 ggcaatttta attcaaagta gcttaaccta tctttttatt gggcgagtag tcgtggtgga
     1561 tattttacgc tccatcgctt ttggtttaat ttctgcgcct tttgtgcttt atttcttcac
     1621 gttattggtg gaaaaacttg aaatatctcg cttgaaatta gaaaaagcag taagagattt
     1681 ggaagaactc cgggaacaag atgcctatct taacgctaaa ttagaaaaaa acagccgtga
     1741 taaaagcatt ttaatggcga ccattagtca cgaacttcgt actcccctta atggaattat
     1801 cggcttgagc caaattttgt tagatgataa aaccaccgaa aaacagcaag aatatctaaa
     1861 aactatcaat atgagtgcca ctgcattggc tcatattttt ggcgatatta ttgatttaga
     1921 aaaaattgat agtagccgaa ttgaattggt aagaaaagag gtggaatttg aacaactcat
     1981 tcacgatatt gctaattttg ggcattttat ggcgggcaat aagcagatta attttgaagt
     2041 gaagtatccg cctgatttgc cgaagtatat ctatatcgac cacgcacgct tgagccaaat
     2101 tttatggaat ttgatcagca atgcggtgaa atttacccct gcaagcggtc gaattttgct
     2161 ggaaatttac caattagata aagaacagtt tagctttgcg gtgacggatt caggcatcgg
     2221 tattccggaa aaagatcaac gcaaaatttt tgatatgttc taccaagcgg aaaactctaa
     2281 agagaaaaaa gcgcaaggta gcgggattgg cttatcggtc tcgaaacgca ttgcgaagct
     2341 aatgggcggt gatttggtgg tggcaagcca agaaaatcaa ggctcaactt ttaccctcac
     2401 tgtgcaggca gaaagcacgg aaaccaagcc gatatttgct attcagcaac attcccttaa
     2461 ggtgctattg gtagaagaca ttgaaatgaa tgtggtggtt gcccgagcaa tgttggagaa
     2521 aatcggttgc gtggttgatg tagcaatgag tggtgaagaa acgttccaac attttgcaca
     2581 gaacagctac gatctgattt tacttgatat tcaactgccg gataccaccg gttttgaaat
     2641 tgcacagcaa ttaagattgc aatatgaaaa cgaggaaatt gactatttac cgctgttggt
     2701 tgctttgacg gcaaatgtga tccaaagccg gcaggagtac caagcacaag gtatggacga
     2761 tgtgctacac aaaccgctct ctttagatga attaatgcgt tgtctcaacc aacattttgg
     2821 agatgatttt ttgcaaaaaa atgaagaaaa acagccgctt gtagagagag gttctgagaa
     2881 tttaccttat agcattacaa ttcttaatga attgattggg gtaatgggga aagagaatgt
     2941 gcttgccaat atggatttat ttgctgattt aatgcccact tatttggcaa atttacagcg
     3001 ttatttaagt gattggcaaa ccacaaattc tgtggaaagc cgtaaattaa cggcggacga
     3061 ggctcataag attaagggag cattagcatc agtcggatta catcagctac aacaaactgc
     3121 tcagcttgct caatccgata acggcgagca gtgggagcaa aatattgcac actgggtgga
     3181 aatgatttgt tccaaatggc agatggattt gcaattagct aaggaatatg ttagaagcaa
     3241 gggggacatt tagtaaattt gaaatgttac gatggtgcaa gcgtccacgc ttgtaccaat
     3301 tttaatatct gaagcacaag cgtggacgct tgtgctatca aaacgtattt accatttctt
     3361 ggttttcggt tttacaaatt caatgccttt atctttatag gtaatatctt ccagctgtaa
     3421 tagaaaacgc ttattcggca agccaccacc gaatccggtt aaggcttgat ttttccctaa
     3481 taccctatgg caaggcacta aaatcgaaat cgggtttcgc ccaactgcac cgccgacggc
     3541 tcgcatcgca cttggcttgc ctagttgggt ggcaatttcg ccataagtag tagtttgccc
     3601 gtaagggatt tcacgcaaaa ttttccaaac tgattgctgg aatggagtgc cttgcggggc
     3661 aagaaaatcc aactgctgaa aatccaatgg ctcgccggca aaatagcggt ctaaaatttc
     3721 tttggttttg caaaaaattt ctaaaatctg accacttgca gcttgagcgg aaaggaaatt
     3781 atcaagcgag gtggtttgtt gctcttgccc tagctcaatg taaatcaagc cttcttctgt
     3841 ggctaaaagc aataattgcc cgatagggct tgggtagtaa tcgtagtaaa tttgagattt
     3901 catgttttta tgccaataaa aaaagaggtg taattgattt tacacctctt taaaccttaa
     3961 ttcatcaaat tagaatttgt agtttacgtt taaaccgtaa agatttgcac gagctttgct
     4021 cttgaatgtg gctaaaccgt cttcagagaa ggtcaggttg ctaccacgaa gatgtgagta
     4081 acctacatca gcagataggt ttggcgtgaa acgcacggtt gcacctacag aataccaagc
     4141 acgatcagtg tccggaattg agattgatgg atgagttaca cttgcacttt catcaaatgc
     4201 tacacctgta cgcaatgtta gcatttcatt cacatcataa gacaagccta atgcaatacg
     4261 ttgagagtca ttaaagttct cggttttgtg caataggcgg ttaccttgca tatcataagc
     4321 ttctaggctt tggaatttgc tccagtctgt gcgtttatag ctgtattgtg cagcaagttt
     4381 atcggttagt ttgtggtaac ctgacacttc ccagaatgcc ggtaggcgta atcttagtgt
     4441 accatcaatc tctgcaccgc cggtaatggt atctaccatc ttaggaataa ctacaggaac
     4501 ggtattagaa tatttgcctt taaatttaag atcaatttca gagtggtaag taaaacctaa
     4561 acggtttctt tcattgatat tataaaccaa gccaacattc caacccaatg cccatttatc
     4621 gcctttaagt ttatgaattg ttgaacttgc attggtattt tgaatgccat taaccaaacg
     4681 ttctaattga gcagaattcg tgatggtttg gtttaaattt tgagatttta ataaaccgtt
     4741 tacttgagct aatacttgtg ttttttgagc ttcaagagcg gtattacctg ctgcgatacc
     4801 tgcctcaaga gcttttaaac cttgaattgc actttgtact tgtactttct tgtcttccaa
     4861 aaattgttta gctggagcaa aacgtgccgc accggcacct aagaaacgtc ctaattctgc
     4921 ttttgcgtgt actgcattca aacctgcccc taatacaaaa ccataaccta aatcataagc
     4981 cgcactaaaa ttaacattca cagcacttaa tgatgtacga ccacctaaga aaccagcaga
     5041 atagttaggg tcaaattcag atatcaaacc ataatttaca tttacgccac caccaataga
     5101 aaggcgctca ttgataggtg aaacgatata agcatttgga acaatagcgg taggaatgac
     5161 atttttatgt gaggcatctg cacctgtagc attgagcttt ccatcaatat ttacatcagc
     5221 atctacgaaa ataccaccgg cagaaatttc ggtttgttta aataacgtca ttaacgcagg
     5281 gttggttgca acaacagaag cgttatcggc tacagcggca ttacctgcgt aagctctacc
     5341 taaaccggat gttgaaattt cagctaattg gaatgcggca gcagatgcac cggctgagct
     5401 gaatgctact aatgaagcga ttaaagtttt gctaaagttt ttcattttca taggaaatct
     5461 cttaaaaaat atatatttta gaatgaataa ttcataaaag ttataagaag aaagtgcaga
     5521 ttttatgtgt cgaagtactc tctatcaatt aaatttcaac tgttccgatc agttaggagc
     5581 aaataagagt gaaattgtgc ggtctatgaa ctgtttatct ttggtatttt ggtattttcg
     5641 gctctttttt cgtataatca gcgttattgt tttaatctgt tttaatatat aaaacctttt
     5701 acactaatta gtcctaaatg attgactaaa ttggtggggt atatcaaaat ttagcaactg
     5761 tttcagaaga ttttctggat aatcaatgag gatattttat gaagcttacc tcaaaagggc
     5821 gttatgcagt aaccgccatt ttagatattg ctttacacgg aaaactagag ccggttagct
     5881 tagccgatat ttcagatcgc caagcgattt cactgtctta tttagagcaa ctgtttgctc
     5941 aacttcgccg tgacggtatc gtgcagagcg ttcgtggacc gggtggtggg tatcaattag
     6001 gtaaaaaacc ggaagagatt agcgtgggta tgatcatctc cgccgtgaat gaaaatattg
     6061 atgtcaccaa atgtaaaggt agtggtaatt gccgtaaaga ttctcagtgt ttaacgcatt
     6121 ctttgtggaa gcggttagaa gatcaaattg agcagttttt agggacaatt tcagttgcgg
     6181 atttagccgc agaacacgct gatcacgatt gtgaaaaaga gcattgtcac gatcatcctc
     6241 atcatcacta attgcaaatt ttttatcaaa aatgaccgct tgggttgttt taactaggag
     6301 taacaatgaa attaccaatt tatttggatt acgcagcaac cacaccaatg gacgaacgtg
     6361 ttgcgaaaaa aatgatggaa tatatgacca aagacggtat ctttggtaac cctgcttctc
     6421 gctcacacaa atttggttgg gaagcggaag aagcggttga tgtggctcgt aaccaaattg
     6481 ccgatttaat cggtgcagac agccgtgaaa tcgttttcac ttctggtgca acagagtcgg
     6541 ataacttagc catcaaaggt gcggctcatt tctatcaaac caaaggtaaa cacattatca
     6601 ctgttaaaac cgaacataaa gcagtattag atacttgccg tcagttagag cgtgaaggtt
     6661 ttgaagtgac ttacttagac cctgaagaag acggtatttt agatttagct aaattaaaag
     6721 cggctattcg tccggacaca attttagttt cggtaatgca cgtaaataac gaaatgggcg
     6781 taatccaacc gattaaagag atcggtgcaa tctgccgtgc gaagaaaatc attttccacg
     6841 ttgatgcgac ccagtctgtt ggtaaaatcg aagtgaatgt acaagactta aatgttgatt
     6901 taatgtcttt ctcaagccac aaattatacg gaccaaaagg tatcggcggt ctatatgttt
     6961 gtcgtaaacc acgtgtgcgt ttagaagcaa ttattcacgg tggcggtcac gagcgtggta
     7021 tgcgttcggg tacattgcct gttcaccaaa ttgtaggtat gggtgaagca tatcgtatcg
     7081 ctaaagaaga aatggcaacg gaaatggctc gtttaaccgt gttacgtgat cgtttatata
     7141 acggctttaa agatattgaa gaagtatatg taaacggttc aatggaagaa ggcaaacgta
     7201 tcggttcaaa cctaaacatc agctttaact ttgttgaggg cgaatcgtta atgatgtcat
     7261 tacgtgatat cgcagtatca tcaggttcag cttgtacttc tgcaagttta gagccatctt
     7321 atgtgttacg tgcaatcggt cgtgacgatg aattagctca cagctcaatc cgtttcactt
     7381 taggtcgttg gacgactgaa gaagaaatcg atcatactat tgaaatcgtg aaaaaagcta
     7441 tcgtgaaatt gcgtgagctt tcgcctcttt gggaaatgtt caaagaaggt gtagatttgt
     7501 ctaaaatcga atggaatcac cactaattca gaaaaatttt gcaagggcaa ataatatttg
     7561 cccctacaaa ataagctttc atcacaaata gtaaattgaa tgtaggggcg aaataggttt
     7621 cgccggaaat aggaaataaa aggaatacaa aaatggcata tagcgaaaaa gtaattgatc
     7681 attacgaaaa cccacgcaac gtagggactt ttgataaaga agcatcagac accggtacag
     7741 gtatggtggg agcaccggct tgtggtgata ttttacgttt acaaattaaa gtgaacgatc
     7801 aaggtatcat tgaagatgca cgctttaaag catacggttg tggttctgca atcgcatcaa
     7861 gttctctcat taccgaatgg gtaaaaggta aatcattaga ggaagcaggt gcgattaaaa
     7921 acagtgatat cgctgaagag ttagaattac caccggtaaa agttcactgt tcaatcttag
     7981 cagaagatgc aatcaaagct gcaattgcag actacaaaga gaaacaagct aagtaatatg
     8041 atacaatcaa tgcaagcggt cgatttttgc aaaaaatttg caaaatttaa ccgcttgcat
     8101 ttgctatttc tatcttgatt taatccattc acataaggaa aactatgagc attaccttaa
     8161 cagaagctgc cgcaaaccga gttcgtacat tcttagaaaa cagaggtaaa ggtatcgggc
     8221 taaggcttgg tgtgaaaact tccggttgtt cggggcttgc ctatgtgctt gaatttgttg
     8281 atgtattaaa tgaagacgat caggttttcg aacaacacgg cgtaaaagtt attgtcgatg
     8341 aaaaaagcct gacttactta aacggtacgc agctggattt tgtgaaagaa ggcttaaacg
     8401 aaggctttaa atacaccaac ccgaatgtta aaaacgaatg cggctgtggc gaaagtttta
     8461 gtgtgtaagg aactgtaatg aataaccctt ttactttatt tgatttaccc gttcaatttc
     8521 aacttgataa cgcccagctt tctgagcgtt atcttgcttt acagaaacag cttcacccag
     8581 ataactttgc ccacaaatca agtgctgaac agttagaagc ggtgcaaaaa tctgctgatg
     8641 tcaatgaggc actcaatatt cttaaagatc cgattttacg ggcagaagcg atgattgaaa
     8701 tccatacagg tgaagccaaa aatctcgaag aaaaaagtat gcgagatgtg gatttcttaa
     8761 tgcagcaact cgaatggcac gaaaaactgg aaaccattga gcatcgtaaa gacgaaaccg
     8821 aactgaccgt ctttttaaaa cagattaaag cggaacaaaa agcagtatta gaacagcttg
     8881 aacaaacgtt aaatacagcc gactggcaaa aagccaatgc gttaaccgac aagctccgtt
     8941 actttaaaaa attaattatt cagattgaga aagtagaaga aaaattcttt ga
//
LOCUS       DANLUQ010000102         8971 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.102, whole genome shotgun sequence.
ACCESSION   DANLUQ010000102 DANLUQ010000000
VERSION     DANLUQ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8971
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.102"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..269
                     /locus_tag="PXB27_001802"
     CDS             <1..269
                     /locus_tag="PXB27_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346565.1"
                     /translation="YNTLKQIMRFRNRLYHQEVVWNKRIAKKPTHALENLEKTYKQFE
                     LALQKIAPERFAFRQLSQALIWQRQIFFDQQIFEAEIVILPQHI"
     gene            complement(305..901)
                     /locus_tag="PXB27_001803"
     CDS             complement(305..901)
                     /locus_tag="PXB27_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346566.1"
                     /translation="MKKLLMTLCCLPAFALANETGESCSKIEDSAKRLECYDSVFVKK
                     DTGKDEATDEKSNWEYEQKKDELRNATTYFAKIRSTNTIDFGFPYNSSSMNLMLRKDP
                     KYGNDVIFSVHGQFNGCMIESCKITVKFDDGKLESYRMIGADGGSNDTLFIENAKAMK
                     TFVDKLKKSKKLIVEASFYNYGKGQFTFDTQGLEWKHF"
     gene            1547..1732
                     /locus_tag="PXB27_001804"
     CDS             1547..1732
                     /locus_tag="PXB27_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346567.1"
                     /translation="MNKMKQLAPFALLILLLGIVGRMDYDDHIQMQRYKCERNQGFWQ
                     MESNGNQYCGGKYGTKN"
     gene            1716..1913
                     /locus_tag="PXB27_001805"
     CDS             1716..1913
                     /locus_tag="PXB27_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346568.1"
                     /translation="MARKIDAGVEIVEVDKRFIAKFFYDGKLQHTTYPQYNRQNAIML
                     INRKIERFNIGRDKPIKLYKE"
     gene            1916..2890
                     /gene="ssb"
                     /locus_tag="PXB27_001806"
     CDS             1916..2890
                     /gene="ssb"
                     /locus_tag="PXB27_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL5346569.1"
                     /translation="MAGINKVIIVGRLGNDPELRTMPNGDSVAKISVATSTEWTDKTS
                     GEKKQATEWHSIIAFRNLADIIGKYLKKGSQVYVEGKLRTRKWQAQDGTDRWTTEIIA
                     DQLQMLGSSNSGNSNNNWATEPAGNPPPTNPYNHVMTDSESRDFDDDIRVRWITKEQA
                     KQFINNISTDWMKTICSFALLTGARRTEILSMTWDKIDFIRKVAIVSNDIAKSEKARS
                     LLLNDDAIELLQKQRGKNPKYVFVGSKGQALQDINRKIFNKATEKCLLVDFHFHDLRH
                     TWASWHVQAGTPLFTLKELGGWETLEMVKKYAHLNADHLLNHANSVKF"
     gene            complement(2977..3053)
                     /locus_tag="PXB27_001807"
     tRNA            complement(2977..3053)
                     /locus_tag="PXB27_001807"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3017..3019),aa:Arg,seq:tct)
     gene            complement(3078..3154)
                     /locus_tag="PXB27_001808"
     tRNA            complement(3078..3154)
                     /locus_tag="PXB27_001808"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3118..3120),aa:Pro,seq:tgg)
     gene            3324..4184
                     /gene="folD"
                     /locus_tag="PXB27_001809"
     CDS             3324..4184
                     /gene="folD"
                     /locus_tag="PXB27_001809"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HDL5346570.1"
                     /translation="MTAQIISGSEVSSQIKKKVSEKIAHYLEQGKRNPGLAVILVGND
                     PASQVYVGSKRKSCAEIGIASKSYDLPEETTEAELLALIEKLNQDDSVDGILVQLPLP
                     KQIDSTKVIEAILPHKDVDGFHPYNVGRLSQRIPTLRPCTPYGVMKLLETTGVDLYGQ
                     HAVIVGASNIVGRPMALELLLGGCTVTVTHRFTKDLEHHVRQADILVVAVGKPEFIPG
                     EWVKDGAIVIDVGINRGADGKLKGDVEYSVASQKAAFITPVPGGVGPMTVAMLMQNTL
                     QTYENHLQAV"
     gene            4298..5749
                     /gene="thiI"
                     /locus_tag="PXB27_001810"
     CDS             4298..5749
                     /gene="thiI"
                     /locus_tag="PXB27_001810"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDL5346571.1"
                     /translation="MKFIIKLFPEIMIKSDSVRKRFIKILTSNIRNVLLKETENVSVV
                     RHWDFIEVRAKVDEETALVLDLLKRTPGIHHILEVEETPFTDMHDIFEQTYAAVKAEL
                     EGKTFSVRVRRKGKHDFRSLDVERYVGGGLNQRIETAKVKLTNPDVTVRIDIEHDKML
                     LIKARHEGLGGYPIGTQEDVLSLISGGFDSGVSSYMLIRRGSRVHYCFFNLGGAAHEI
                     GVKQMAYHIWSRYSTSHKVRFIAINFENVVGEILEKVDNGQMGVVLKRMMVRAASRIA
                     ERFDIQAIVTGEALGQVSSQTLTNLRLIDKASDTLVLRPLITHDKEQIIAMAKAIGTD
                     DIAKSMPEFCGVISKNPTVKAIETKILEEEGNFNFEVLEQAVQTASYLDIREIAQQTE
                     KEVVSVETTTELSKNDVVLDIRSPEEVDEKPFRLDGVEVKELPFYKLSSQFSTLDQSK
                     TYLLYCQRGVMSKLQALYLKENGYSNVKVFRVK"
     gene            complement(5794..6501)
                     /locus_tag="PXB27_001811"
     CDS             complement(5794..6501)
                     /locus_tag="PXB27_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwiC-like family protein"
                     /protein_id="HDL5346572.1"
                     /translation="MFKEKPVISNQHGALAMAMLPFLYAVIESGFSTLHIFFGLSWLF
                     LYFFSYPFLSLFSKKPTERNKKWAKIYFGLSILFALPLIYAQPSILQFLPIILPLGLV
                     QVHYAKQKDERNLWNDIAGILTFGIMGMAAYYLVTKQYQFEILLHPTLFFIATTFYIK
                     SMVRERRNPIYMEISIAIHLVLAMLYILCSLQAVFFAYLIATARAIIVPSFGWNVKQV
                     GMLEFPVILLFLASLIW"
     gene            complement(6569..7543)
                     /gene="pfkA"
                     /locus_tag="PXB27_001812"
     CDS             complement(6569..7543)
                     /gene="pfkA"
                     /locus_tag="PXB27_001812"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDL5346573.1"
                     /translation="MSKKIKKIAVLTSGGDAPGMNAAIRGVVRTALNEGLEVCGIQDG
                     YYGLYHDKVIPLDRRSVSETINRGGTFLGSARFPDFKRPEVRKHCVETLKKYSIDALV
                     VIGGDGSYMGAKLLTEEFGYPCIGIPGTIDNDIVGTDYTIGYQTALETALDAIDRLRD
                     TSSSHQRISIVEIMGRHCGDLTLSAALAGGCEYVIVPEKGLDKDALMRSIEEGFHKGK
                     RHSIIAITELMTDVHALAKEIEARFGNETRATVLGHIQRGGSPCAFDRILASRMGVYA
                     VELLLQGFGGHCVGIQNEQLVHHDIIDAINNMRRPFKEELFEASRKLF"
     gene            complement(7650..8846)
                     /locus_tag="PXB27_001813"
     CDS             complement(7650..8846)
                     /locus_tag="PXB27_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL5346574.1"
                     /translation="MLNTQFRTHFPFFEKSDGWTYLDSAATTLKPDVLIEATSEFYAS
                     AGSVHRSQYDLPQTEQYELARDLVAKRFNVETREAVIWTSGTTQSINTVAYGLEHQIA
                     AGDEIVISVAEHHANFIPWQQLAQRTRAKLVVLPLNDKYEIELETLKNALSEKTKIVA
                     FNLVSNVTGVRQPAEQIIPLIRQSCSAKIVLDIAQAVCSEQVDVQKLDADFYAFSAHK
                     MYGPNGVGVLTGKLQSLEQIQPLVFGGKMLKNITESELTVAELPYRLEAGTPNIAGII
                     GFGQVLAWLEKWDFVALNHQLYDLAEYTCKRLNSYQNLQIIGQQNTPTISFVVKNQHP
                     ADIAAFLTQSKIAIRQGEHCAKPYLRHLEEVGTVRLSLAHYNNQQDVEHFFNALDFTL
                     EILQES"
BASE COUNT         2800 a         1752 c         1863 g         2556 t
ORIGIN      
        1 tatacaatac attaaagcaa attatgcgtt ttagaaatcg gctgtatcat caagaagtgg
       61 tttggaataa gcgaattgcc aagaaaccaa cgcacgcact agagaattta gaaaaaactt
      121 acaagcaatt tgagttagca ctacaaaaga tagcacctga acgatttgcg tttcgtcagc
      181 tttcacaagc acttatttgg caacgccaaa tctttttcga ccaacaaatt tttgaagctg
      241 aaatcgttat tttgccccaa catatctaag caaaaagcta cgcatctatg tagctttttc
      301 ttaattaaaa atgtttccac tctaaccctt gtgtatcaaa ggtaaattgc cctttgccat
      361 aattgtaaaa actcgcctct acaattagtt ttttcgactt tttcaactta tctacaaagg
      421 ttttcattgc ttttgcattt tcaatgaaaa gcgtatcatt gctgccacca tccgccccaa
      481 tcattcgata actttccaat ttaccatcat caaacttcac agtaatttta cagctctcaa
      541 tcatacagcc attaaattga ccgtgtacgc taaaaattac atcattgcca tatttagggt
      601 ctttgcgtaa cattaaattc attgatgatg aattatacgg gaaaccaaaa tctattgtgt
      661 tagttgatct aattttagca aagtaagtcg ttgcattacg caattcatct ttcttttgtt
      721 catattccca attagacttt tcatcggtag cttcatcttt accagtatct tttttcacaa
      781 agacactatc ataacattct aagcgttttg cactatcttc aatcttagag caactttctc
      841 cagtttcgtt agctaaagca aaagcgggca aacaacataa agtcatcagt agttttttca
      901 tataacctcc atagaaatcc cttcttattt tctcaaaagc gtggttttcg gtctgtgatc
      961 aagctcgcaa tctcaataat tatattttaa tggtttaaaa aacaatcaat taagaaatca
     1021 taactaattg aaatttaaga aatcttaaaa atacttatta aataagctta aattttgatt
     1081 gacttgtaat ttaagttttc ttaaacttaa ttcatcaaaa cgagctacgg ctcaatgctc
     1141 tttaacaatt cggtgcttgt gcggacgata ttaacaacct cgggcagttg taaagtaatg
     1201 ctttataact cagtaaggtt ggttagaccc tgacatacaa atttgaacga cttgccaaaa
     1261 agaggtggca aggtttaggc aacacactcg caaggtgtgg agagctgaaa aggcaacact
     1321 ggtatcagtc ctaagcaatc acgcttatct caagtttgag taagtacgga tacaagcagc
     1381 acccgacgga atggcgaaag ccgggcgggc gacggcgtgg gcagacacgc atttattcca
     1441 aagctcattt caccaagtgt gtttcggaat acccacaact agacgcaagt cgctccattt
     1501 gcccaccgta aaacgtgggc tttttttaac cctaaaggac aatcctatga acaaaatgaa
     1561 acaacttgcc ccatttgcct tgctaatttt gctattaggc attgtcgggc gaatggatta
     1621 tgacgaccac atacaaatgc aacgttacaa atgcgaacga aatcaaggct tttggcaaat
     1681 ggaaagcaac ggcaaccaat actgcggagg gaaatatggc acgaaaaatt gatgccggtg
     1741 ttgagattgt tgaggtagat aaacgattta tcgccaagtt tttttatgac ggcaaattac
     1801 agcacacaac ctatccacaa tacaaccgcc aaaacgcaat tatgttgatt aaccgcaaaa
     1861 ttgagcggtt caacattggc agagacaaac caatcaaact ttataaggaa taacaatggc
     1921 aggaattaac aaagtgatta ttgtcggtcg cctaggtaac gacccggaac tacgaacaat
     1981 gccaaatggc gattcagtcg ccaaaatcag tgtagcaact tcaaccgaat ggacggataa
     2041 aacctcaggc gagaaaaaac aagccacaga gtggcatagc attatcgcct ttcggaattt
     2101 agctgacatt atcggcaaat acctgaaaaa aggctcgcaa gtgtatgtgg aaggcaagct
     2161 acgcactcgc aaatggcaag cccaagacgg cacagaccgc tggacgaccg aaatcatcgc
     2221 cgatcagcta caaatgctcg gtagctcaaa cagcggaaac agcaataata attgggcaac
     2281 cgaaccggca ggaaacccac caccaaccaa tccatataac cacgtaatga cggattcaga
     2341 atcacgagat tttgatgatg atatccgggt tcgttggatt accaaagaac aagccaagca
     2401 gtttattaat aatattagta ctgattggat gaaaactatc tgctcttttg ctttattaac
     2461 aggagcaagg cgaacagaaa tactttctat gacttgggat aaaatagatt ttatccgtaa
     2521 agtagcgatt gtttcgaatg atattgccaa atctgaaaaa gcgagatcgc ttttacttaa
     2581 tgatgatgca attgaattat tacaaaagca gagagggaaa aatcctaaat atgtttttgt
     2641 aggaagcaaa gggcaggcat tacaagatat taaccgcaaa atatttaaca aagcgactga
     2701 aaaatgttta ttggttgatt tccatttcca tgatttaagg catacttggg ccagttggca
     2761 cgttcaggcc ggcacaccat tatttacctt aaaagaattg ggtggctggg aaacattaga
     2821 gatggtaaag aaatatgccc atcttaatgc agaccattta ctcaaccacg ctaactcagt
     2881 taaattctag tcacaaatta gtcacaaagt atttttgaca taggaaatta ataactgata
     2941 tttaaatgta actatttgaa atataagggt aattaatggc acgccctaaa ggattcgaac
     3001 ctttgaccca cgccttagaa gggcgttgct ctatccagct gagctaaggg cgcagatgaa
     3061 gataatataa aaagaagtgg tcggcgagat aggatttgaa cctacgaccc actggtccca
     3121 aaccagttgc gctaccaagc tgcgctactc gccgacataa gatgttgttg attatagatt
     3181 tctttgcaca acagtcaatc tttttttgtg aatgtcggct taaatgattg aagtttatcc
     3241 gttattcaaa attagcttat caaaaaggct attttttgag agaatgacga atgttttgcc
     3301 aagtgatatt tcgggaggta gtaatgactg cacaaattat ttcggggagt gaggtttctt
     3361 cacaaattaa aaaaaaggtg agtgagaaaa tcgctcatta tcttgaacag ggtaaacgta
     3421 accctggtct tgcggtcatt ttagtgggga atgacccagc ctctcaagtg tatgtgggga
     3481 gtaaacgcaa aagctgtgcc gaaattggga ttgcgtcgaa atcttatgat ttacccgaag
     3541 aaaccacaga ggctgaatta ttagcactga ttgaaaagtt gaatcaagat gattcggtcg
     3601 atgggatttt agtccaatta ccgttaccta aacagatcga ttcgactaaa gtgattgaag
     3661 ccatcttacc acataaagat gttgatggat tccaccctta taatgttggg cgtttatctc
     3721 agcgtattcc cactttacgc ccttgcacgc catacggtgt gatgaagttg cttgaaacaa
     3781 ctggggtaga tttatatggt caacacgcag tgattgtagg tgcgtctaat attgttggtc
     3841 gcccgatggc gttagaattg ttgcttggtg gttgtacagt cacagttact catcgcttta
     3901 ccaaagattt agagcatcac gttcgccaag cagatatttt agttgtggca gtcggcaagc
     3961 ctgaatttat tcccggggag tgggttaaag acggtgctat cgtgattgat gtagggatta
     4021 accgtggagc agacggcaaa ctaaaaggtg atgtagagta ttcagttgcc tcacaaaaag
     4081 cggcatttat cacccctgtc cccggtgggg taggcccgat gacggtggca atgttaatgc
     4141 aaaatacctt gcagacgtat gaaaaccatt tacaagcggt ctaattttac ggatattttg
     4201 caaatcctcg gcattggtcg aggatttctt tttctgggct ttttcagcag aaaaggctaa
     4261 aatacttgcc atttttatta cacgatcctt attaaatatg aaatttatca tcaaactttt
     4321 ccctgaaatt atgattaaaa gcgactctgt tcggaaacgc tttatcaaaa ttttaacctc
     4381 aaatattcgc aacgtattat taaaagagac tgaaaatgtg tctgtggttc gccattggga
     4441 ctttattgaa gttcgggcga aagtggacga agaaacggca ttagtgctgg atttgcttaa
     4501 acgcacgccg ggtattcacc atattttaga agtggaggaa acccctttta cggatatgca
     4561 cgatattttt gagcagactt atgccgcagt gaaagctgaa ctagaaggca aaacgtttag
     4621 cgtgcgggtt cgccgtaaag gaaagcacga tttccgttct cttgatgtag agcgttatgt
     4681 cggtggtggg ctgaatcagc ggattgaaac ggcgaaagtg aaattaacca acccagatgt
     4741 tacggtcaga attgatattg aacatgataa aatgttgctg attaaagctc gccacgaagg
     4801 gttaggcggg tatccaatcg gcacacaaga agatgtgttg tcattgattt cgggtggttt
     4861 tgattcgggt gtatcgagct atatgctgat tcgcagaggt tctcgtgtgc attactgttt
     4921 tttcaattta ggtggtgccg cacacgaaat tggcgtgaaa cagatggcat atcatatttg
     4981 gagccgttat agtacctcgc acaaagtccg ttttatcgcc attaattttg aaaatgtagt
     5041 gggtgagatt ttagaaaaag ttgataacgg acaaatgggc gtggtattaa aacgtatgat
     5101 ggtgcgagcg gcaagccgta ttgccgagcg ttttgatatt caagcgattg tgaccggtga
     5161 ggcgttgggg caggtctcta gccaaacact gactaattta cgtttaattg ataaggcatc
     5221 agatacgctg gtattacgtc cgctcatcac tcacgataaa gagcagatta ttgcaatggc
     5281 aaaagcgata ggcactgacg atattgctaa atcaatgccg gagttctgtg gagtaatttc
     5341 taaaaatccg acggttaagg caatcgaaac taaaattttg gaagaagaag gcaactttaa
     5401 ttttgaggta ctagaacaag cggttcaaac ggcgagttat ttagatattc gtgagatcgc
     5461 tcagcaaacc gaaaaagagg ttgtttcggt tgaaaccaca acagaattga gtaaaaacga
     5521 tgttgtgcta gatattcgca gcccggaaga ggttgatgaa aaaccattcc gtttagacgg
     5581 ggttgaagta aaagaactcc cgttttataa actttccagc cagtttagta cgcttgacca
     5641 aagcaaaacc tacttgctct attgccaacg tggggtaatg agtaaactgc aggctcttta
     5701 tttgaaagag aatggctaca gcaatgtaaa agtgtttcga gtgaaataat gcctctttga
     5761 ttgcacctta atgattgatt aaggtgcaat tttttaccaa ataaggcttg ctaaaaagag
     5821 taagatcaca ggaaattcaa gcattccaac ttgtttaaca ttccagccaa agcttggcac
     5881 aataatggct cgggcagttg caattaaata agcaaaaaag accgcttgta gagagcagag
     5941 tatgtaaagc attgctaaaa ccaaatgtat tgcaatactg atttccatat aaattgggtt
     6001 tcttcgctct cgcaccatac tctttatata gaaagtcgta gcaataaaaa atagggtagg
     6061 gtggagcaga atctcaaact gatattgttt cgttaccaga taataagctg ccattcccat
     6121 tatgccaaag gttagaatgc cggcaatatc attccataaa ttgcgttcat ctttttgttt
     6181 ggcgtagtga acttgcacta acccaagcgg tagaattatc ggtaaaaatt gcaaaatcga
     6241 aggctgagcg tagatcaaag gtaatgcaaa tagaatgctt aagccaaagt agattttcgc
     6301 ccatttttta ttgcgttcag ttggcttttt actgaaaagt gagagaaaag ggtaggaaaa
     6361 gaagtagaga aacagccacg acagcccaaa gaaaatatgc aaagtgctaa aaccactttc
     6421 aataacggcg tagagaaacg gcaacattgc cattgctaat gcaccgtgtt ggttggatat
     6481 aacgggtttt tctttgaaca ttcaatttcc ctcttaatgt ggcaaataaa aaggcgacta
     6541 aacagccgcc tttaaattgt aagaaaagtt agaataattt tctagaggct tcaaataatt
     6601 cttctttgaa cggacgacgc atattattga tcgcatcaat aatatcgtgg tgaaccaact
     6661 gctcgttttg aataccgaca cagtgaccgc caaagccttg cagtaataat tccaccgcat
     6721 agacgcccat acgggaggcc aaaatacggt cgaaggcaca aggcgaacca ccacgttgaa
     6781 tatgacctaa tacggttgca cgggtttcat tgccgaaacg tgcttcaatt tctttcgcta
     6841 atgcgtgaac atcggtcatc aattctgtaa ttgcaataat tgaatggcgt ttacctttgt
     6901 ggaaaccttc ttcaatgctt cgcattaatg catctttatc taagcctttt tccggtacga
     6961 tcacatattc gcaaccaccg gcgagggcag cacttagggt taaatcacca cagtggcgac
     7021 ccataatttc cacaatcgaa atacgttggt gtgaacttga agtatcacgc aggcggtcaa
     7081 tcgcatctaa tgcggtttca agggcagttt gatagccgat ggtgtaatcc gttcccacaa
     7141 tatcgttatc aatcgtaccc ggaataccga tacaagggta accaaattct tcggttaata
     7201 atttagcccc catataagaa ccgtcgccac caataaccac taatgcatca atgctgtatt
     7261 tttttaaggt ttcgacacaa tgtttgcgaa cttctggtct tttaaaatca gggaagcggg
     7321 cagaacctaa gaatgtaccg cctcggttga tggtttcaga cacggaacgg cggtcaagtg
     7381 ggatcacttt atcgtggtaa agcccatagt agccgtcttg aataccacaa acctctaaac
     7441 cttcattaag tgcggtacga accacaccac ggattgccgc gttcattcct ggtgcatcac
     7501 caccactggt taatactgca atttttttga tttttttact catttaaaaa caccttgttg
     7561 ataaattaaa gttaaattgg aaactggtgc aaggttacaa tattcagcct tttcgttcta
     7621 ctaaaaaata cgtatttttt tgatttagct tagctttctt gcaaaatttc aagggtaaaa
     7681 tctagagcgt tgaaaaaatg ctcaacatct tgttgattat tgtaatgggc aagtgaaagt
     7741 cgaactgttc cgacctcttc taaatggcgt aaataaggct tggcacaatg ctccccttgg
     7801 cggatcgcaa ttttactttg ggttaagaaa gcggcaatat cggcagggtg ctgatttttc
     7861 accacaaagc taatagtcgg tgtattttgt tgcccgataa tttgcaaatt ttgataagaa
     7921 tttaaccgct tgcaggtgta ttcagccaag tcataaagct gatgattaag agcgacaaaa
     7981 tcccattttt cgagccaagc tagcacttgc ccaaagccga taatgccggc gatattcggc
     8041 gtgccagctt ccaagcgata aggtaactct gccacagtta attcactttc cgtaatgttt
     8101 ttcagcattt tgccgccaaa tacaagcggt tgaatttgct ccaaactttg caatttgccg
     8161 gtaagcacgc ccacaccgtt tggaccatac attttatggg cggaaaaagc gtagaaatct
     8221 gcatctaatt tctgaacgtc cacttgctct gagcaaactg cttgagcaat atctaacaca
     8281 attttagcag agcaagattg gcggataagc gggattattt gttccgctgg ctggcgaact
     8341 cctgttacgt tagaaactaa attaaaggca acaattttgg ttttttccga taacgcattt
     8401 ttaagggttt caagctcaat ttcatactta tcattaagtg gcaacaccac gagtttcgcc
     8461 cttgtacgct gtgccaactg ctgccaagga ataaaattag cgtgatgttc cgccactgaa
     8521 attacaattt catcgcctgc tgcaatttga tgctccaagc cataagccac ggtgttaata
     8581 ctttgggttg taccgcttgt ccaaatcacc gcttcacgag tttccacatt gaaacgtttt
     8641 gcgactaaat ctcgagctaa ttcatattgt tcggtttgtg gcaaatcgta ctgactgcga
     8701 tgcaccgacc ccgcagaagc gtaaaactca cttgttgctt caatgagtac atcaggtttc
     8761 agcgttgtag cggcagaatc taaatacgtc catccgtcag atttttcaaa aaaagggaaa
     8821 tgggtgcgaa attgggtgtt aagcataagt taattcctaa gagttgtgga aaattgagtt
     8881 accaagaaaa tacctattat aaaggaatgt tttgaggtaa gaagcatttc aagaaataaa
     8941 tagatttatt ttgttatctt tttgttaaat t
//
LOCUS       DANLUQ010000103         8968 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.103, whole genome shotgun sequence.
ACCESSION   DANLUQ010000103 DANLUQ010000000
VERSION     DANLUQ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8968
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.103"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..6051
                     /locus_tag="PXB27_001814"
     CDS             <1..6051
                     /locus_tag="PXB27_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HDL5346575.1"
                     /translation="IFNQTTQKMEVVSELARSQGKAASSTDKRGTIKLAAASILGGAT
                     LISGMAAFMLSATPAEAAVIIDRIEVQQKAPKKVVNGGIDIYTNTSLATDSITNPYNY
                     FNPGSLSYADRTKLQESNNSVNKEKDSRLLYKSNTTSAIVIGHNAVALDNNRKSRGKP
                     KASGIAIGDYSYSGGGLSIAIGSFSRTASIGSVALGTSARAEGFNSFAAMRQSAAIAD
                     YSTAIGSTSWANATASFAIGASATALGDRSFAIGSGTPQTVDGSYGHENRRSIYDGQN
                     NTLAFGTDAFSIGTKAKTNGNHSFAIGTNAWSGGFKLENSNISELGQVINPDTAKVAN
                     NAFALGTNTRANSNDTLAFSTNASATGDGSIAFGVNSNTTGIGAIAFGNASKAWKSNA
                     ISIGWNANSHAEKVISIGSHSNASKDGAMAIGDTANSSGNNSVAIGYSANVSKANATA
                     VGTRANITHEGGTAIGFEAKSKGNYATAIAYNSTAKGKNSIAAGVNSTVSGIRAAALG
                     YNNTVSGKDTFVLGSNVTVKTKGSVILGSDSTEATATKEDTATVGTLKYSSFAGDENV
                     VDGDYVSVGNATNARQIKFVAPGKIASNSTDAINGSQLYAVADTLVKTGFNITADHSG
                     LGGGAKEDNVKLGETVKYTSTDKNVVTTVTNNTIDFGLSDNISVGGKPGKDGSVGVKG
                     ADGKDGVTIKPEAIVFHGVDGVNGKDGKDGKASIKVEKGAKGLDGNDGKDGESKTRIV
                     YEKPNGDKEEVATLNDGLKFGANDGKVHDAKLNTQVDIKGATTNTDWAKFDAGKNIMT
                     QVVGNTITVALAKDLAGLDSATFGTQGDSTVINKDGVTIAKKDPNDATKSNVTLTEDG
                     LSNGGNQITNVDSGLKGKDGQPVKLTEAAGDVLNNAVNVGDLKNTVTETVTEVTTDAF
                     GLKDENNKEFKQNLGTTAKIIGDGNVKTNVVEKDGGHALEVSLVNNITVGKDGEPGKV
                     GIAGKDGKDAIALDGKDGAITVGRPGKDGIDGTIGVNGKDGSAVVLNGKDGSIGLNGT
                     NGKDGLTIKGTEGKPGLVGKDGLPGKDGVTRIQYQPTDGNGDPIGEPEQVATLNDGLH
                     FKGDDATLVSKKLNETLEIIGGADKDKLTANNIGVNSKDGKLSVQLAKDLKDLNSATF
                     KPQDGENGQEGKGPTTVVNNNGVTITPKDGTDGKAVKLTEDGLDNGGNQIVNVKSGID
                     GLAGKDGINGKDGKPITSVNDLTDDQLKEVGTNAANINDVVNASNDIIEKGFSIAADN
                     GETDHVKLGETVKYTSKDKNIITTVSDNVIDFTLNNDFTIGGPGKDGSIGVKGADGTV
                     GVTLNGKDGSIGLTGPKGADGKDGASATISVKDGAKGLDGNDGKDGESKTRIVYEKPD
                     GTTEEVATLNDGLRFVGDDGKEIVKKLNETLKISGGDTDLTNLSDNNIGVVNNNGALT
                     VKLAKDIDLTKDGSVTIGDTTVNNDGLTIKDGPSVTKAGINAGDKKIINVQDGEISAT
                     SKDAVNGSQLYQVQEVANAGWNLTANGKDKGNVKPGATVDLNNKDGNIIITKEGNNVT
                     FGLKNNLTIGGKDGKDGQIGIAGKDGKDGVTIKGDGTITAGRDGKDGVDGSIGATGKD
                     GASVVLNGKDGSIGLTGPKGADGKDGASANIAVKDGAEGVDGTNGKDGLPGENGKTRI
                     VYETKDKDGKTVTEQVATLNDGLIFSGNNEDVKNRQKLNTEVKVKGEGVDKTASENFK
                     SASGNINVKANGTDTLEVQLAKAIDLTEDGSVTTGDTTVNNNGLTIKDGPSVTKGGID
                     AKGTKITNVKDGDVTATSKDVVSGSQLYNAMQNTGWYLTVDGNVAETSEKGAQRVNNN
                     GKVAVSGGKNIVVSRKGSTVEVATSSNLEFDSVKVGGTTISSTVAKDGVNELNIAGSK
                     NAQTRITNVAPGVKGTDAVNVNQLKGAENRLNNRISNVDKDLRAGIAGALAAGNLYHV
                     TQPGKSMVSAGVGAYRGQGAIAVGYSRLSDNGKVGIKFSVNSNSRGDTGAAASVGYQW
                     "
     gene            complement(6273..7658)
                     /gene="hflX"
                     /locus_tag="PXB27_001815"
     CDS             complement(6273..7658)
                     /gene="hflX"
                     /locus_tag="PXB27_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDL5346576.1"
                     /translation="MLNESLPNSEENSAFGFALSEASQTTEAASPTDPENQDKAILVH
                     LYLGQHKDVENLLEFKTLAESAGVEVLATLTTSRQSSHIKYYVGQGKAEGIAEAVERL
                     GATVVLVNHQLSPAQTRNLQSLCGCRVVDRTGLILDIFAQRARSHEGKLQVELAQLRH
                     LSTRLVRRVTNQDQQKGGAVGLRGPGETQLETDRRLIKVRIQQLLNRLEKVSKQRNQN
                     RKTRQKADIPTVSLVGYTNAGKSTLFNAITQASVYAADQLFATLDPTLRRIQVQDVGT
                     TILADTVGFIRFLPHDLVSAFKSTLQETTEATLLLHVIDGSDERKNENIDAVNQVLDE
                     IGALDIPTLLVFNKIDKLEGVEPHIERNADGTPVAVYLSAQENSGIELLFQAIQERLR
                     SNLVCENLLLPVTAGAIYAQFKAENCIKTEHFNEFGERIISIEINEVQWNKWVKQFPD
                     LAEYIELAKWG"
     gene            complement(7678..7950)
                     /gene="hfq"
                     /locus_tag="PXB27_001816"
     CDS             complement(7678..7950)
                     /gene="hfq"
                     /locus_tag="PXB27_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005712219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HDL5346577.1"
                     /translation="MAKGQSLQDPYLNALRRERIPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPARALSHNNNNQAQHAQPAAEVTEQAE"
     gene            complement(8019..8942)
                     /gene="miaA"
                     /locus_tag="PXB27_001817"
     CDS             complement(8019..8942)
                     /gene="miaA"
                     /locus_tag="PXB27_001817"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011271810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDL5346578.1"
                     /translation="MGPTASGKTDLAIALRQELPVEVISVDSALIYKGMDIGTAKPSK
                     EELALAPHRLIDILDPAESYSAMNFHADALREMADITSQGKIPLLVGGTMLYYKALLE
                     GLSPLPSADPAIRAEIESRAAQHGWATLHQELVKIDPIAGARINPNDSQRINRALEVF
                     YITGKTMTELTAQQGEALPYNILQFAIAPQDRAVLHQRIEQRFHKMIELGFEEEVKRL
                     FLRKDLHINLPSIRCVGYRQMWEYLQGDISLDEAIFKGICATRQLAKRQITWLRGWQG
                     ELTWLDSLAPTSSKAKMVEKIDHYLKNCDNI"
BASE COUNT         2785 a         1608 c         2102 g         2473 t
ORIGIN      
        1 atttttaacc aaacgacgca aaaaatggaa gttgtgtctg aacttgctcg atcgcaaggt
       61 aaagccgctt cttctactga taagcgcggt actattaaat tagcagcggc tagcattcta
      121 gggggagcaa cccttatcag tggaatggca gcctttatgc tttctgctac accggcagaa
      181 gcggcggtga ttattgatag aatagaagta caacaaaaag caccaaaaaa agttgttaat
      241 ggtggtatag atatttatac aaacactagt ttggctacag attctattac gaatccatat
      301 aactacttta acccaggtag cttatcctat gcagacagaa ctaagcttca agaatctaat
      361 aactctgtta ataaggagaa ggattctaga ttattgtata agtctaatac tacctcagca
      421 attgtaattg gacacaatgc tgtagcttta gacaataaca gaaaaagtag aggaaaaccg
      481 aaagcgtcag gcatagccat tggtgattat tcatattctg gagggggatt gtctattgca
      541 attggttctt tttctcgtac tgcaagtatt ggttctgtag cattaggtac ctctgcacgt
      601 gcggaaggat ttaactcatt tgctgcaatg cgtcaatctg ctgcaattgc agattattct
      661 actgcaattg gttcaacatc ttgggcaaat gctacagcta gcttcgccat tggtgcttcg
      721 gctacagcgc taggtgaccg ttcttttgcg attggtagtg gtactccaca gacagtggat
      781 ggaagttacg gtcatgaaaa tagacgttca atttatgatg ggcaaaataa tactttagca
      841 tttggaacag atgcattctc aatcggtact aaagcgaaaa ctaatggtaa ccattcattt
      901 gccattggta ctaacgcttg gtccggtggc tttaaattag agaattcaaa tatttctgaa
      961 ttaggacagg taattaatcc tgatacagcg aaagtagcaa ataatgcatt tgctttgggt
     1021 acgaatactc gtgcaaattc gaatgatact ttggcgtttt caacaaatgc aagtgcaaca
     1081 ggtgatggtt ctatcgcctt tggtgtaaat tcgaatacaa caggaatcgg tgcgattgct
     1141 tttggtaatg cttctaaagc ctggaaaagt aatgctatta gcattggttg gaatgcaaac
     1201 tctcatgcag aaaaagtgat cagtattggt tcgcattcta acgcgagcaa agatggtgca
     1261 atggcaattg gtgatacggc taattcaagt ggtaataatt ctgttgcgat aggttatagt
     1321 gctaatgtaa gtaaggcgaa tgcgacagca gtaggtacac gtgccaatat tacgcatgaa
     1381 ggtggcacag caattggttt tgaggccaaa tcaaaaggta attatgcaac tgctattgct
     1441 tacaactcaa ccgcaaaggg taaaaactca attgcagccg gtgtaaatag tactgtttcg
     1501 ggtattcgtg ctgcggcttt aggttataac aatacagttt caggtaaaga tactttcgta
     1561 ttaggttcta atgttactgt taagacaaag ggttctgtaa ttctaggttc tgactctact
     1621 gaagcaaccg caactaaaga agatactgca actgttggca cattaaaata tagcagcttt
     1681 gctggcgatg aaaatgttgt tgatggtgat tatgtgagtg tgggtaatgc aaccaatgct
     1741 cgccaaatta agtttgttgc accgggtaaa attgcttcca attcaacaga tgcgattaac
     1801 ggttcacaac tttacgcagt agctgacact ttagttaaaa ctggctttaa cattacagcg
     1861 gatcactctg gcttaggagg tggtgctaaa gaagacaatg tgaagttagg tgaaactgtt
     1921 aagtacacct caaccgataa gaatgttgtt acaaccgtaa caaataatac catcgatttt
     1981 ggcttatccg ataacatctc tgttggtggt aaaccaggta aagatggttc agttggtgtt
     2041 aaaggtgctg atggtaaaga tggtgtaaca attaaaccag aagccatcgt attccacggc
     2101 gttgatggcg taaatggaaa agatggaaaa gatggtaaag catcaattaa agttgagaaa
     2161 ggtgctaaag gtcttgacgg caacgacggt aaagacggcg aaagcaaaac tcgtattgtt
     2221 tacgaaaaac caaatggtga taaagaagaa gttgcaacac ttaacgatgg cttaaaattc
     2281 ggtgcgaatg atggcaaagt tcacgatgcg aaattaaata cgcaggtgga tattaaaggt
     2341 gctacaacaa acactgattg ggctaagttt gatgctggta aaaatatcat gacacaggtt
     2401 gttggcaata ccattactgt ggcgttagca aaagatctag caggcttaga tagtgcaaca
     2461 ttcggcacac aaggtgattc tacagttatc aataaagatg gtgtaacgat tgctaagaaa
     2521 gatcctaatg atgcaactaa atctaatgtt accttaacag aggatggctt aagcaacggt
     2581 ggtaaccaaa tcaccaatgt tgatagcggc ttaaaaggta aagatggtca acctgttaaa
     2641 cttactgagg cagcaggtga tgttcttaac aatgcggtta acgtcggtga cttgaaaaat
     2701 actgttactg agactgttac tgaggtgaca acagatgcgt ttggtctaaa agatgagaac
     2761 aacaaggagt ttaagcaaaa cttaggtacg acagctaaga tcattggtga tggcaatgtt
     2821 aaaacaaacg ttgttgagaa agatggtggc catgcattag aagttagcct agtgaataat
     2881 attactgttg gtaaagatgg tgaaccaggt aaagttggta ttgctggtaa agacggtaaa
     2941 gatgctattg ctttagatgg taaagatgga gctatcactg taggtcgccc tggcaaagat
     3001 ggtattgatg gtactatcgg tgtaaacggt aaagacggtt ctgcagttgt tcttaacggt
     3061 aaagatggtt caatcggctt gaacggtaca aatggtaaag atggtttaac cattaaaggt
     3121 actgagggca aaccagggct tgttggtaaa gatggtttac caggtaaaga tggtgtaact
     3181 cgtatccaat atcagccaac agatggcaat ggtgatccta taggtgaacc agagcaagta
     3241 gctactttaa atgatggttt acactttaaa ggtgatgatg caacactagt atctaagaaa
     3301 ttgaatgaaa ccttagaaat cattggtgga gctgataaag ataagctaac tgcaaataac
     3361 atcggcgtaa acagcaaaga tggtaaatta tctgttcaat tagctaaaga tcttaaagac
     3421 ttgaactcag ctacattcaa accacaagat ggagagaatg gtcaagaagg taaaggtcca
     3481 acaacagtag taaataataa tggcgtaact atcacgccaa aagatggcac agatggtaaa
     3541 gcagttaagc ttactgaaga cggtctagac aatggcggca accaaattgt taacgtgaag
     3601 agcggtattg acggtttagc aggtaaagac ggcattaacg gtaaagatgg taagccaatc
     3661 acttcagtga acgacttaac agacgatcaa ttaaaagaag ttggtactaa cgctgcgaat
     3721 attaatgatg ttgtaaatgc aagcaacgat attatcgaga aaggctttag cattgcagcg
     3781 gataatggtg aaactgatca cgttaaacta ggtgaaactg ttaagtacac ctctaaagac
     3841 aaaaacatca tcacaacagt aagtgataat gtgattgact ttacgttaaa taacgatttt
     3901 accattggcg gtccaggtaa agatggttca atcggtgtta aaggtgcaga tggtacagtt
     3961 ggtgttactt taaacggtaa agatggctct atcggcttaa ccggtccgaa aggcgctgac
     4021 ggtaaagatg gtgcaagtgc aactatctct gtaaaagacg gtgctaaagg ccttgacggt
     4081 aacgacggta aagatggcga aagcaaaacc cgtatcgttt atgagaaacc agacggtaca
     4141 actgaggaag ttgcaacact gaacgatggc ttaagattcg taggtgatga cggtaaagaa
     4201 atcgttaaga aacttaatga gacgttaaaa atctcaggcg gtgatactga tttaacgaac
     4261 ctgtccgata acaatatcgg tgttgtaaac aacaatggtg cgttaacagt taaattagcg
     4321 aaagacattg atttaacgaa agatggttct gtaactattg gtgatactac ggtaaataat
     4381 gatggtttaa ctatcaaaga tggcccaagt gtaactaaag ccggtatcaa tgcaggcgat
     4441 aagaaaatca ttaacgttca agacggtgaa atttctgcaa ccagcaaaga tgcggtaaat
     4501 ggtagccagt tatatcaagt gcaagaagtt gctaacgcag gttggaactt aacagcaaat
     4561 ggtaaagaca aaggcaacgt taaaccagga gcaacagttg atctaaacaa caaagacggt
     4621 aacattatta ttactaaaga aggtaataac gtgaccttcg gcttgaaaaa caacttaacc
     4681 attggtggta aagatggtaa agacggtcaa atcggtattg ctggcaaaga cggtaaagat
     4741 ggcgtaacca ttaaaggtga cggcacaatt actgcaggcc gtgacggtaa agacggtgtt
     4801 gatggttcaa ttggagctac aggtaaagat ggcgcttctg ttgtattaaa cggtaaagat
     4861 ggctctatcg gcttaaccgg tccgaaaggc gctgacggta aagatggtgc aagtgcaaat
     4921 attgcagtga aagatggtgc tgaaggtgtt gatggtacaa atggtaaaga tggattaccg
     4981 ggtgagaacg gtaagactcg tatcgtttat gagaccaaag ataaagacgg taaaactgtc
     5041 acagaacaag ttgcaacctt aaatgatggt ttaatcttct ctggtaacaa tgaagatgtg
     5101 aagaaccgtc agaaactgaa tactgaagtt aaagtgaaag gtgaaggtgt ggataaaaca
     5161 gcttctgaaa actttaagtc agcatcaggc aacatcaatg tgaaagcgaa tggtacagac
     5221 acattagagg tacaattagc gaaagcaatt gatttaacgg aagatggttc tgtaacaact
     5281 ggcgacacaa ccgtaaataa caatggttta accattaaag acggcccaag tgtaactaaa
     5341 ggcggtattg atgcgaaagg cacgaagatc actaacgtta aagatggtga tgtaacggca
     5401 acaagtaaag atgttgtgag cggtagccag ttgtataatg caatgcagaa tacaggctgg
     5461 tatctcactg ttgatggtaa tgtagctgaa acttcagaga aaggtgctca acgtgttaat
     5521 aacaatggta aagtggccgt ttcaggtggc aaaaacatcg ttgtttcacg taaaggttca
     5581 actgttgaag tggcaacctc aagcaatctt gaatttgatt cagtgaaagt tggtggtaca
     5641 actatcagtt caactgttgc aaaagatggc gtaaatgagc ttaacattgc aggtagcaag
     5701 aatgcgcaaa cacgtattac gaacgttgct ccgggtgtga aaggcactga tgctgttaat
     5761 gtgaatcagt tgaaaggtgc agaaaatcgt ctgaacaatc gtatcagtaa cgtagataaa
     5821 gacttacgtg caggtattgc aggtgcatta gccgcaggta atttatatca cgtaacacaa
     5881 cctggtaaat cgatggtttc agccggtgta ggtgcttacc gtggtcaagg agctattgct
     5941 gtaggttatt ctcgtttatc agataatggt aaagtgggta tcaaattcag tgttaactct
     6001 aacagccgtg gtgatacagg tgctgctgca agcgtaggtt atcagtggta gtaactgctt
     6061 gattaagctt ttcttcccca cttttgggga agaataaata aaatccttca aatagaacgg
     6121 ggaaatctgg tttatttgag ggattttttt attggctaaa ttggatttaa tggataaaac
     6181 aagcggtcaa ttctttacac ttttttgcaa aaaaacgtaa agaattgacc gcttgcataa
     6241 tttaaatata acttactcag aagtgagatt aattaccccc attttgccag ctcaatatat
     6301 tcggctagat ccggaaattg tttcacccat ttgttccatt gcacttcgtt aatttcaata
     6361 gagataatcc gttcaccgaa ctcattaaaa tgctcggttt taatgcagtt ttctgcttta
     6421 aattgtgcgt aaattgcacc ggcagtaacc gggaggagca agttttcaca cactaagtta
     6481 ctgcgaagcc tttcttgaat ggcttggaat aacagctcaa tgccgctatt ttcttgggcg
     6541 gaaagataca cggcaactgg cgtgccatcg gcattgcgtt caatatgcgg ttctacacct
     6601 tctaatttat caattttatt gaataccaat aaagtaggaa tatctaacgc tccgatttcg
     6661 tccaacactt gatttaccgc atcaatattt tcgtttttgc gttcgtctga gccgtcaatg
     6721 acgtggagca gtaaagtagc ttcggtggtt tcttgcaggg tagatttaaa ggctgaaact
     6781 aaatcgtgcg gtagaaaacg gataaaacct accgtatcgg caagaattgt cgtgccgaca
     6841 tcttgcacct gaatccggcg taaagtcggg tcaagggtcg caaaaagttg atcggcagca
     6901 taaacactag cttgggtaat cgcattaaac agggtagatt tgccggcatt ggtatagccc
     6961 actaaggata ctgttggaat atcggctttt tgtctggttt ttcgattttg attgcgttgc
     7021 ttgctgactt tttccaagcg gttgagtaac tgttggattc gcactttaat caaacggcga
     7081 tcggtttcga gctgtgtttc acctggacca cgcaagccaa ctgctccgcc tttttgttgg
     7141 tcttgattag taactcggcg aaccaaacgg gtcgataaat ggcgaagttg tgccagctcg
     7201 acttgtagtt tgccttcgtg cgatcttgcc cgttgagcga aaatatccaa aatcaagccg
     7261 gttctatcca ccacacggca gccgcataac gattgcaaat tacgggtctg agccgggcta
     7321 agttgatggt tcaccagcac tactgttgcc ccgagtcgtt ctaccgcttc ggcaattccc
     7381 tccgctttgc cttgccccac ataatatttg atatgcgaac tttggcggct ggtggtaagc
     7441 gttgccagca cttcgacacc ggcagattct gccagcgttt taaattccag caaattttca
     7501 acatctttat gttgcccgag atagagatgc accaaaatgg ctttatcttg attttctgga
     7561 tcggtaggcg aggcggcttc tgtagtttgg cttgcttcag ataaagcaaa gccaaacgca
     7621 gaattttctt ctgagtttgg caatgattcg tttaacattg gttgagtaat aatgttatta
     7681 ttccgcttgt tcagttactt cggcagcagg ctgagcgtgt tgggcttggt tgttattgtt
     7741 atgcgataac gcacgggcag gaacaaccgt tgaaatagcg tgtttataca ccatttggct
     7801 tacagtgttt tttaataaaa tcacaaattg gtcgaacgac tcgatctgac cttgtaattt
     7861 aatgccgtta actaaataaa ttgaaacagg aatacgctca cgacgaagtg cattcaaata
     7921 tggatcttgt aaagattgac cttttgccat tgttattttc ctttttatag ttgtgtgatt
     7981 tttaagacac caaataatgc gataagtgcc aaaggcgttt agatattatc acaattcttt
     8041 aaataatgat cgatcttttc caccattttt gctttagaac tggtcggagc taaactatct
     8101 aaccaagtca gttcgccttg ccagccacgc agccaagtaa tctgccgttt tgccagctgg
     8161 cgggtggcac aaatgccttt aaagatcgcc tcatcgagcg aaatatcgcc ttgcaaatat
     8221 tcccacattt ggcggtagcc cacacagcga atcgagggca aattaatatg caaatctttg
     8281 cgtaaaaata accgcttgac ctcctcttca aagcctagct caatcatttt gtgaaagcgt
     8341 tgttcaatcc gttggtgtag aactgcacga tcttgaggag cgattgcaaa ttgtaagata
     8401 ttataaggta gagcctcacc ttgctgagca gtgagttcgg tcatcgtttt gccggtaatg
     8461 taaaaaactt ctaacgctcg gttgatccgt tggctgtcat ttgggttaat tcttgcaccg
     8521 gcaatcgggt cgattttcac taattcttga tgcaatgttg cccagccgtg ttgtgccgct
     8581 cggctttcga tttcagcccg aatggctgga tcggcagagg gcaggggaga taacccttca
     8641 agcaatgcct tgtaataaag cattgtgcca cccaccaaga gcggaatttt accctgtgag
     8701 gtaatatctg ccatttcacg caacgcatca gcgtggaaat tcatcgccga atagctctcg
     8761 gcagggtcta aaatatcaat caagcgatgc ggtgcaaggg caagctcttc cttgcttggc
     8821 tttgccgtgc caatatccat tcctttgtaa attaaggcag aatcaacact gattacctca
     8881 accggcaatt cttggcgtaa agcaatagct aaatcggttt tcccggaggc agtcggcccc
     8941 attaaaaaaa gggcaagggg ttttttat
//
LOCUS       DANLUQ010000104         8936 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.104, whole genome shotgun sequence.
ACCESSION   DANLUQ010000104 DANLUQ010000000
VERSION     DANLUQ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8936
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.104"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            22..768
                     /gene="znuC"
                     /locus_tag="PXB27_001818"
     CDS             22..768
                     /gene="znuC"
                     /locus_tag="PXB27_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HDL5346579.1"
                     /translation="MVSLENIEVSFNGQKALQNINLSIYPNSITTIVGPNGGGKSTLL
                     KILLKLLSPNKGKVIHQKGLKIGYVPQKLHLEPSMPITVYRFLALKPHTTKEMINQAL
                     DLFSITHLTENSMQKLSGGELQRVLLARAILDRPQLLVLDEPMQGVDIIGQTELYQLL
                     NKTRDLLNCAILMVSHDLNIVMANTDEVLCINKHICCAGSPEVITHDPSFIHFFGDQF
                     ARNVAFYSHKHNHHHNLHGDICLGECSCEH"
     gene            933..3746
                     /gene="sucA"
                     /locus_tag="PXB27_001819"
     CDS             933..3746
                     /gene="sucA"
                     /locus_tag="PXB27_001819"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HDL5346580.1"
                     /translation="MQYTHFDDWLASSALGGANQSYIEEIYEQYLENPANVDESWRNI
                     FDSFPKTPQVEQAHTPIRDYFRKLAREQQPQSVTIIDPEAGAKQVRLLQWINAHRFRG
                     YLEAKLDPINYYRWKTSSVPELDYKYHGFTDEDLNETFTVGRYVYDKDTMKLSELDKA
                     LKETYLGPIGLEFMHVQDIEQRNWLQAKMESVLNKPLFTKAEKLNLLSELTAADGLER
                     YLGAKFPGAKRFSLEGSDAFIPMMKEIIRHASRQGMTDVVLGMAHRGRLNMLVNVFGK
                     RPAELFDEFAGKHADESRTGDVKYHQGFSSNFAVDDKNVHLTLAFNPSHLEIVSPVVI
                     GSVRARQERMRDIEHEKVLAVTVHGDSAVAGQGVVQETLNMANVRGYKVGGTIRIVIN
                     NQIGFTTSNPNDTRSTEFCTDIAKMIQAPIIHVNGDDPEAVAFAARMAVEYRTKFKRD
                     IFIDLISYRRHGHNEADEPLATQPMMYSIIKKHPTPRKVYADRLITEGVLTNEQEIEM
                     LNLYRDALDNGECVVPEWREMDMAAVDWLQYLNYDWTALYESKFPEERFLTIAKRVCE
                     YPASLKAHSRVEKIYTDRKEMYEGKKLFDWGMAETMAYATLLDEGTHVRLSGEDAGRG
                     TFFHRHAVIHNQNDGTGYVPLTQLHANQGRFQVWDSVLSEEAVLAFEYGFATTDPKTL
                     TIWEAQFGDFANGAQIVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARVERY
                     LQLCAQQNIQVCVPSTPAQVYHMLRRQAIRKMRRPLVAITPKSLLRHPLAVSSLDELI
                     NGSFQNVIGELDNIDPKQVKRVVMCSGKVYYDLIEKRRENNQTDVAIIRIEQLYPFPL
                     EEMQQILEPYAHVKDFVWCQEEPLNQGAWYCSKHNFEASLPEDAKLTYAGRPASASPA
                     VGYPSLHAKQQKQLVEDALTIAE"
     gene            3849..5078
                     /gene="odhB"
                     /locus_tag="PXB27_001820"
     CDS             3849..5078
                     /gene="odhB"
                     /locus_tag="PXB27_001820"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HDL5346581.1"
                     /translation="MTIEILTPDLPESVADATVATWHKKVGDNVKRDEILVEIETDKV
                     VLEVPASSDGILAEITQEQGATVVSKQSLGKLVVAKAGDISSATIEQKTESTPSDRKH
                     AAIENSHANADDQGPAIRRLLAEHDLQAADIQGSGVGGRITREDIEKEIAKRVQINQV
                     KGSATSQNTVSTVAFNSRSEKRVPMTRLRKRIAERLLEAKNTTAMLTTFNELDMQPIM
                     NLRKTYGEKFEKQHGVRLGFMSFYIKAVVEALKRYPEINASIDGDDVVYHNYFDISIA
                     VSTPRGLVTPVLRDCDKMSMADIEKKIKELAEKGRDGKLTVEDLTGGNFTITNGGVFG
                     SLMSTPIINPPQSAILGMHAIKDRPVAIDGQVVIRPMMYLALSYDHRLIDGKESVGFL
                     VAVKELLEDPTRLLLEI"
     gene            5371..6531
                     /gene="sucC"
                     /locus_tag="PXB27_001821"
     CDS             5371..6531
                     /gene="sucC"
                     /locus_tag="PXB27_001821"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HDL5346582.1"
                     /translation="MNLHEYQAKQIFKQYGLPVSQGIACKTAEEAVEAIKQLQGEQWA
                     IKCQVHAGGRGKAGGVKLVRNEAEVREFADKWLGKRLVTFQTDANGQPVNTLYLEETC
                     NIDRELYLGAVVDRSAQKVIFMASSAGGMNIEEVAEKTPELIHKAVIDPLTGGQGYQG
                     RELAFKLGLKGDEVKQFADIFVKLANLFVEKDLALLEVNPLVITKEGQLLCLDAKMSI
                     DSNALYRHPELKELQDPSQDDEREAEAEKWNLNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLYGGKPANFLDVGGGATKERVAAAFKLILSDPNVKAVLVNIFGGIVRCDLIADGVIA
                     AVNEVGVNVPVIVRLEGTNAAQGREILANSGLSILAASSLKQAAELAVKSAN"
     gene            6540..7409
                     /gene="sucD"
                     /locus_tag="PXB27_001822"
     CDS             6540..7409
                     /gene="sucD"
                     /locus_tag="PXB27_001822"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HDL5346583.1"
                     /translation="MSILIDKNTKVICQGFTGGQGTFHSEQALAYGTQLVGGVSPNKG
                     GTTHLGLPVFNTVREAVEATGATATVIYVPAPGCKDAILEAIDAGIQLIVCITEGIPT
                     LDMLQVKQRLNQTGVRMIGPNCPGVITPDECKIGIMPGNIHKKGNIGIVSRSGTLTYE
                     AVKQTTDEGFGQSTCVGIGGDPIPGSNFIDILKLFQDDPETEAIVMIGEIGGSAEEEA
                     AAFIKENVTKPVVSYIAGVTAPKGKRMGHAGAIISGGKGTADEKFKILEDAGVTTVRS
                     LAEIGSALRKILG"
     gene            complement(7480..8925)
                     /locus_tag="PXB27_001823"
     CDS             complement(7480..8925)
                     /locus_tag="PXB27_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HDL5346584.1"
                     /translation="MDSIILFFESVLTWIVNTIDGPLWDVTIILLLGAGLFFTLTTGF
                     VQLRLLPQSLREMWGGRSTEGKSLTPFQAFTTGLASRVGVGNIGGVATAIALGGPGAV
                     FWMWITALMGMSSAFAESSLAQLYKTKDPNGMFRGGPAYYITRGLKLPSLAIAFAVTL
                     IFTFGFAFNAVQSNSIVEATKNAWDWNPVYVGMTLVVITGLIISGGVKRIGKVSAQVV
                     PMMALFYLIIAVIILGMNIEKVPSIISLIIESAFDFSAAAGGTFGALVSKTMLMGIKR
                     GLFSNEAGMGSAPNSAATSDAKHPASQGLIQMLGVFVDTIVVCTCTAVIILMSNNYGG
                     ETLRGISLTQSALQFHLGEFGLHFLAFVILLFCFTSIIGNYAYAETNIRYIKNSPWFV
                     WGFRAIVLFFVYFGAVRDGGIVWAFADTVMASMAVINLVAILILAPIVWIILKDYLRQ
                     VKSGKEPVFRIEEHQDLIHKGVDPLIWKSKE"
BASE COUNT         2790 a         1932 c         1888 g         2326 t
ORIGIN      
        1 cagaatttat ccgcgacatc actggtttct cttgagaata ttgaagtgtc tttcaatgga
       61 caaaaagcat tacaaaatat taatttatct atttatccta actctatcac gacgatagtc
      121 gggccaaatg gtggaggaaa atctaccctg cttaaaattt tgctaaaatt acttagccca
      181 aataaaggaa aggttattca tcaaaaagga ctcaaaattg gctatgtacc acaaaaattg
      241 catttagaac cctcgatgcc cattactgtt tatcgatttc ttgcgctaaa accacatact
      301 accaaagaaa tgattaacca agcactagat ctgttttcta ttacccatct cactgaaaat
      361 agtatgcaaa aattatcggg tggtgaactt caacgtgtat tgctggctcg ggcaatttta
      421 gatcgtccgc aacttttagt gttagatgaa ccaatgcaag gcgttgatat tataggccaa
      481 accgaactct atcaattact taataaaaca agggatctcc ttaactgtgc tattttaatg
      541 gtctcacacg acttaaatat tgtgatggcg aatacagatg aagtgctttg catcaataaa
      601 catatttgtt gtgccggaag ccccgaagtc attacacacg atccgagctt tatccatttt
      661 tttggcgacc aatttgcacg taatgttgca ttttattcac ataaacataa ccatcaccat
      721 aatttacatg gcgatatctg ccttggtgaa tgtagttgtg agcactgata ggcgtccttt
      781 ttaccaaatg tttaaaattt gtaaaaataa tgctaaaaat aaccgcttgc agcacaagat
      841 ttatctaaaa atcagactta cttcacattt tatttgctta aattggagta taataagggc
      901 gtgatttcat accacaaaca ggtcaggtct ttatgcaata tacacatttt gatgattggc
      961 ttgcatcatc tgcattaggc ggtgcaaacc aatcttatat agaagaaatt tatgaacagt
     1021 atctagaaaa tccagcaaat gttgatgaaa gctggagaaa catctttgat tcatttccta
     1081 aaactcccca agttgaacaa gctcacaccc ctattcgtga ctatttccgt aaattagccc
     1141 gtgaacagca acctcaatcc gtcactatta ttgacccaga agcaggggcg aaacaagttc
     1201 gcttactgca atggattaac gctcaccgtt tccgtggcta tttagaggca aaactagacc
     1261 caattaacta ttaccgttgg aaaacctcca gcgtgcctga attggactac aaatatcacg
     1321 gcttcaccga tgaagatctc aacgaaactt ttacggtcgg tcgctatgtc tatgataaag
     1381 acacaatgaa gctcagcgaa ctggataaag ccctaaaaga aacttattta gggcctattg
     1441 gtttagaatt tatgcacgtt caagatattg aacaacgcaa ctggcttcaa gccaaaatgg
     1501 aaagcgtgct aaacaaaccg ctgttcacca aagcagaaaa actgaattta ttaagcgaat
     1561 tgaccgctgc cgatggttta gagcgttact taggggcaaa attcccgggg gcaaaacggt
     1621 tctcattgga aggtagtgat gcctttattc cgatgatgaa agaaattatc cgccacgctt
     1681 cccgtcaagg tatgaccgat gtggttttag gtatggcaca ccgtggtcgt ttgaatatgt
     1741 tagtgaacgt atttggtaaa cgcccggcag aattgtttga tgaatttgcc ggcaaacacg
     1801 ctgatgaaag ccgcacgggc gatgtgaaat accaccaagg tttctcctca aactttgccg
     1861 tagatgataa aaacgttcac cttacccttg ctttcaaccc atcgcattta gagattgtta
     1921 gcccggtggt aatcggctca gtgcgtgcaa gacaagaacg tatgcgtgat atcgaacacg
     1981 aaaaggtgct tgcggtgacc gttcacggtg actctgcagt ggcagggcaa ggtgtagtgc
     2041 aagaaacctt aaatatggca aatgttcgtg gctataaagt aggaggcact atccgtattg
     2101 tgatcaacaa ccaaatcgga tttaccacct ctaacccaaa tgacacccgc tccaccgaat
     2161 tttgtaccga cattgccaaa atgattcagg caccgattat tcacgttaat ggtgatgatc
     2221 cggaagctgt tgcctttgca gcgagaatgg ctgtggaata tcgtactaaa tttaaacgtg
     2281 atatttttat tgatttaatc tcttaccgtc gtcacggtca taatgaagcc gatgagcctt
     2341 tagcaactca gccaatgatg tatagcatca tcaaaaaaca cccgacacct cgcaaagtct
     2401 atgcggatcg tttaattacc gaaggtgttt taaccaacga gcaagaaatc gagatgttaa
     2461 acctgtatcg tgatgcctta gacaacggcg aatgcgttgt gccggaatgg cgtgaaatgg
     2521 atatggcagc ggtcgattgg ctacaatatc tcaattacga ctggactgcc ctatacgaaa
     2581 gtaaattccc tgaagaacga ttcttaacca ttgccaaacg ggtgtgcgaa tatccggctt
     2641 cgcttaaagc tcattctcgg gttgaaaaga tctacactga tcgcaaagag atgtatgaag
     2701 gcaagaaatt attcgactgg ggtatggctg aaacaatggc atacgctact ttgttagacg
     2761 aaggcacaca tgttcgcctt tcaggagaag atgccggacg tggcactttc ttccatcgcc
     2821 atgcggtaat ccataaccaa aatgatggaa caggctatgt gccacttact caactgcacg
     2881 ccaatcaagg gcgtttccaa gtatgggatt ccgtactctc tgaagaagcg gtactcgcct
     2941 ttgaatatgg ctttgccact accgacccga aaacactgac catttgggaa gctcaatttg
     3001 gagatttcgc taacggagct caaattgtta tcgaccaatt tatcagctca ggcgaacaaa
     3061 aatggggcag aatgtgcggt ttagtgatgt tattaccaca cggctatgaa ggtcaagggc
     3121 cggaacactc ctctgcccga gtggaacgct atttacagct ttgtgctcag caaaatattc
     3181 aagtttgcgt gccatcaaca ccggcacagg tttaccatat gttacgccgt caagcgatcc
     3241 gcaaaatgcg tcgcccattg gttgcaatca caccaaaatc attgctacgc cacccacttg
     3301 cggtgtcatc tcttgatgaa ttgattaacg gttcattcca aaatgtgatt ggcgagctcg
     3361 ataatatcga tcctaaacaa gtcaaacgtg tggtgatgtg ttcgggtaaa gtgtactacg
     3421 acttaattga aaaacgccgt gaaaataacc aaacggacgt tgccattatt cgtatcgaac
     3481 agctctaccc gttcccactt gaagaaatgc aacaaattct cgagccttac gctcacgtta
     3541 aagactttgt ctggtgtcaa gaagagccac taaaccaagg tgcgtggtat tgcagcaagc
     3601 acaattttga ggcttctctg ccggaagatg caaaattaac ttacgcaggc agaccggctt
     3661 ctgcttcacc ggcggtgggt tatccatcgt tacacgcaaa acaacaaaaa caacttgttg
     3721 aagatgcttt aacgatcgct gaataataaa atgggcgtat atacgcccta caagcggttc
     3781 acttttaacc aaattttaca atttacgctg tcggagtttt aaaactctct cgaaaaaagg
     3841 aataagttat gaccattgaa attttaaccc ccgatttacc cgaatctgtt gccgatgcga
     3901 ccgttgccac ttggcataaa aaagtgggcg acaatgtaaa acgtgatgaa attttagtcg
     3961 aaattgaaac agacaaagtc gttttagaag taccggcttc aagcgatggt attcttgcag
     4021 aaattaccca agaacaaggt gctactgttg tatccaagca atctctgggt aaattagtag
     4081 tagctaaagc cggtgatatt agctctgcaa ctattgagca aaaaacagaa tccacgccat
     4141 cagaccgcaa acacgcagca attgaaaaca gccacgcgaa cgctgatgat caagggcctg
     4201 cgattcgccg tttattagcc gaacacgact tacaagccgc tgatattcaa ggcagtggtg
     4261 taggcggcag aatcacccgc gaagatattg aaaaagagat tgccaaacga gtgcaaatca
     4321 accaagtaaa agggtctgca accagccaaa acaccgttag cactgtagca tttaacagcc
     4381 gtagcgaaaa acgtgtgccg atgacacgac tccgcaaacg tattgccgaa cgcttactag
     4441 aggcaaaaaa caccactgca atgctcacta cttttaacga gctggatatg caaccaatta
     4501 tgaatttacg caaaacctat ggcgaaaaat tcgaaaaaca acacggtgtg cgtttaggct
     4561 ttatgtcgtt ctacattaaa gccgtggttg aagcattaaa acgctaccct gaaatcaatg
     4621 cctccattga tggcgatgat gtggtttatc ataactattt cgacatcagc attgcggttt
     4681 ctaccccacg aggcttagtc acacctgtgt tgcgtgattg cgacaaaatg agtatggcag
     4741 atattgagaa gaaaattaaa gaacttgccg aaaaagggcg tgacggcaaa ttaaccgttg
     4801 aagacttaac cggcggtaac tttaccatca ccaacggcgg ggtattcggt tcattaatgt
     4861 ccaccccaat tatcaacccg ccacaaagtg cgattttggg tatgcacgct attaaagacc
     4921 gccctgtcgc cattgacgga caagtagtga tccgcccgat gatgtaccta gccttaagct
     4981 atgaccaccg tttaattgac ggtaaagaat ctgtcggttt cttagtggca gttaaagagt
     5041 tattagaaga cccaacccgt cttcttttgg aaatttaatc gaaaatatga ccgcttgtac
     5101 ttttagctac aagcggtctg tttttaataa aatttgacaa aatccgataa acgctctatt
     5161 tgagttccct tctgagccac ctgagcgact ttgaatttgt aggaaaaatt tagtgtttga
     5221 gcttacaagg caagcgagtt ataaattttt ccgtcaagca aattcaaact aagcgaagaa
     5281 agtggcgaag cagggtgtgc tttcttttgg ttacttttct ttgcacaagc aaagaaaagt
     5341 aacataaaaa aaaccaaacg aggaaactct atgaatctac acgaatatca agcaaaacaa
     5401 atattcaaac aatacgggct tcccgtaagc caagggattg cttgcaaaac ggcagaggaa
     5461 gccgttgagg caattaaaca actacaaggt gaacaatggg cgataaagtg ccaagttcac
     5521 gctggtgggc gtggcaaagc cggcggggtg aaactggttc gcaatgaagc cgaagtgcgt
     5581 gaatttgccg ataaatggct cggcaaacgc ttagttacct tccaaaccga tgctaatggg
     5641 caaccggtta ataccttata tttagaagaa acctgcaata tcgaccgtga attgtactta
     5701 ggtgcagtgg ttgatcgctc cgctcaaaaa gtgattttta tggcatcaag tgccggtggt
     5761 atgaatattg aagaagtagc ggagaaaacc cctgaattaa ttcacaaagc ggtaattgac
     5821 ccactcaccg gtgggcaggg ttatcaaggg cgtgaattag catttaaatt aggcttgaaa
     5881 ggtgatgaag ttaaacaatt tgcggatatt ttcgtgaaac tggctaacct atttgttgaa
     5941 aaagatttag ctttattaga ggtaaatcca cttgtcatca ctaaagaagg gcaactactc
     6001 tgcttagatg caaaaatgtc gattgacagc aatgctctct accgccaccc agaacttaaa
     6061 gagttgcaag acccatcgca agatgacgaa cgtgaagctg aagcggaaaa atggaatctc
     6121 aactacgttg cactagatgg caatatcggc tgtatggtaa acggtgcggg gctggcaatg
     6181 gggacgatgg atatcgtcaa actttatggt ggtaaaccgg ctaacttctt agacgttggc
     6241 ggtggtgcaa ccaaagagcg tgtagcagca gcttttaaat tgattttatc tgatccgaat
     6301 gtgaaagccg tgttagtcaa tatttttggt ggtatcgtcc gctgcgattt gattgctgat
     6361 ggcgtcattg ctgctgtaaa cgaagtcggc gtgaatgtgc ctgttattgt gcgtttagaa
     6421 ggcactaatg ccgcacaggg gcgtgagatt ttagccaata gcgggctgag tattctagcg
     6481 gcatcatcat taaaacaagc ggctgaatta gcggtaaaat ctgcaaatta ggaggaaaaa
     6541 tgtcgatttt aattgataaa aacacaaaag taatttgcca aggtttcacc ggcggacaag
     6601 gtactttcca cagcgaacaa gcccttgctt atggcacaca attagtcggc ggagtttcgc
     6661 cgaataaagg tggcacaacc catttaggct taccggtttt caacaccgta cgcgaagcgg
     6721 tagaagcaac cggtgcaact gcaaccgtta tctacgttcc ggctcccggc tgtaaagatg
     6781 ctattttaga agccattgat gccggcatcc aactaattgt ctgtattact gaaggcattc
     6841 caaccttgga tatgctacaa gttaaacaac gtctaaacca aaccggcgtg cgaatgatcg
     6901 gtccgaactg cccgggtgtg attacccctg atgagtgcaa aatcggcatt atgccgggca
     6961 atatccacaa aaaaggcaat atcggtattg tttctcgttc gggtacttta acctatgaag
     7021 cagttaaaca aacaactgac gaaggctttg gtcaatctac ttgtgtcggt atcggtggtg
     7081 atccgattcc gggatctaac tttattgaca ttttaaaact gttccaagac gatcctgaaa
     7141 ctgaagcaat tgtgatgatt ggtgaaatcg gcggctctgc cgaagaagaa gcggcagctt
     7201 tcatcaaaga aaatgtcacc aagcctgtag tcagctatat tgccggtgtc accgcaccaa
     7261 aaggcaaacg aatgggacac gccggtgcga ttatcagcgg cggcaaagga accgccgatg
     7321 aaaaattcaa aatccttgaa gatgccggtg tgacaaccgt cagaagttta gccgaaatcg
     7381 gttcagctct tagaaaaatt ttaggctaaa ctaaaagttc aagcagtcat atttacccat
     7441 ttttttgtaa atatgaccgc ttgttttttt gctattaaat tactctttcg atttccaaat
     7501 taatgggtca acacctttat ggataaggtc ttgatgttct tcaattctaa acactggctc
     7561 tttgccactt ttcacttggc gaagatagtc ttttaaaata atccagacaa tcggagcaag
     7621 aattaagata gccaccaagt taattaccgc cattgatgcc atcactgtat cggcaaatgc
     7681 ccatacaatc cctccgtctc ttactgcacc gaagtacaca aagaataaaa cgatggcacg
     7741 gaatccccac acaaaccacg gactgttttt aatgtagcgg atattagttt ctgcataagc
     7801 atagttaccg ataatggatg tgaagcaaaa cagtaaaatc acaaatgcta aaaagtgtaa
     7861 accaaattca cctaaatgga attgtaacgc actttgagta agtgaaatac ctcttaaagt
     7921 ttcaccacca tagttatttg acataagaat gatcactgca gtacaagtac agactacaat
     7981 tgtatctaca aatacgccca acatttgaat taagccttga cttgccgggt gtttcgcatc
     8041 agaagtcgct gcagaatttg gtgcagatcc cataccagct tcgtttgaga ataagccacg
     8101 tttaatcccc attagcatag ttttggatac caatgctcca aacgttccac ctgcagcagc
     8161 agaaaaatca aaggcgcttt caataattaa agagattata cttggcactt tttcaatatt
     8221 catacctaaa ataattactg caataatcaa gtagaataat gccatcattg gaacaacttg
     8281 ggcagaaact ttaccaatac gcttgacccc tccagaaata attaaccctg taatcaccac
     8341 taatgtcatg cctacgtaga ctgggttcca atcccacgca tttttggttg cttccacaat
     8401 agagttagat tgcacagcat taaaagcaaa accaaaggtg aagataagcg tcactgcaaa
     8461 agcaatagca agagaaggca gttttaaacc acgagtgatg taataggctg gcccaccacg
     8521 aaacattccg tttggatctt tggttttata caactgagcc agtgaagact cagcaaaggc
     8581 ggaactcatt cccattaagg cggtaatcca catccagaat accgcacccg gcccacccaa
     8641 agcgattgca gtagctaccc caccaatatt accaacccca acacggctgg ctaatccagt
     8701 agtaaaggct tggaacggag ttaatgattt cccttccgta gaacgtcctc cccacatttc
     8761 acgtaaactt tgcggaagaa gacgcagctg gacaaaacct gttgtaagag tgaaaaataa
     8821 ccctgcaccg agcaatagga taatcgttac atcccaaagt ggaccgtcga ttgtgttcac
     8881 aatccaagtc agtacactct caaaaaatag aataattgaa tccataaaga cctctt
//
LOCUS       DANLUQ010000105         8892 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.106, whole genome shotgun sequence.
ACCESSION   DANLUQ010000105 DANLUQ010000000
VERSION     DANLUQ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8892
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.106"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            45..1358
                     /gene="pilQ"
                     /locus_tag="PXB27_001824"
     CDS             45..1358
                     /gene="pilQ"
                     /locus_tag="PXB27_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HDL5346585.1"
                     /translation="MWRIFIYFLCGFSLAYANTISIVLKNAPTSLIFTYLAEETGKNM
                     VLDDNIETKSTLRLENKSVDEIFKTLGKVNKLSLTQEDDIVYIHKKEEKAADLTPLPI
                     MNLQNNGQNTPLVTAPKLITKTLKLHYAKASEVIESLTKGSGTFLSENGYIHFDERSN
                     SLIVKDSAKSLKNIENLVKQLDQPTEQIAIEARIVTISSEHLQELGVRWGIFSRGAGH
                     YKFGGRLEGNGLNNVTNNLNVNFPITGGASAVLQVSSINSRVLDLELSALEQENSVEI
                     IASPRLLTTNKKPASIKQGTEIPYVMYNTKSEATDVEFKEAVLGLEVVPHLSTQNQIL
                     LDLIVTQNSPNSQSGSSGLITIDKQELNTQVFAKHGETIVLGGIFQHLTQKGEDKVPI
                     LGSIPFIKRLFSQTRDKISKRELVIFVTPYILQSNEKSSNRKKSK"
     gene            1439..1852
                     /gene="nusB"
                     /locus_tag="PXB27_001825"
     CDS             1439..1852
                     /gene="nusB"
                     /locus_tag="PXB27_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDL5346586.1"
                     /translation="MKISPRRRARECAVQAIYSWYISQNTVEEVELAFVTDQDMKGVD
                     MPYFRKLFRGVVDNVEAIDEALRPYLDRKESDVDPIERSILRLSTYELKFEHDVPFRV
                     VINEGIEVAKVFGSDDSHKYINGILDKLAPALGRK"
     gene            1915..2895
                     /gene="thiL"
                     /locus_tag="PXB27_001826"
     CDS             1915..2895
                     /gene="thiL"
                     /locus_tag="PXB27_001826"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HDL5346587.1"
                     /translation="MGEFDIIERYFNASQRPPRKDVLLSIGDDCALTSLKPNQQLAIT
                     TDTLVCGTHFLPTISPADLAYKSVASNLSDLAAMGAEPAWISLALTLPEINHDWLAEF
                     SEHFFEILDHYNVDLIGGDTTCGPLSLTITAQGIVPQDRGLFRHNAKVGDWIYVSGTL
                     GDSAGGLQLLLNQQSEQVNWNKEQSYLIQRHLRPTPRVLLGLTIASIANAAIDISDGL
                     LSDLSHILKRSDCGAVLNLENFPLSDVLLSCYSRTEAEYFALNGGEDYELCFTVPNDN
                     KAKLDKALANIGVTYTCIGQIRSAKEGLTLLRHNQPVTLPSQRGYDHFKK"
     gene            2900..3373
                     /locus_tag="PXB27_001827"
     CDS             2900..3373
                     /locus_tag="PXB27_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HDL5346588.1"
                     /translation="MKPINLKNPIHFFAFGFGSGLLKPAPGTWGSLAGLIVAIALWSI
                     TQSALFFGLLTLVSFIAGCVICTKASQDLEVHDDGRIVWDEIVAIFLMFTCLPEYNLL
                     GYLLAFISFRIFDILKPFPIRYFDEKLESGLGIMFDDILAAIFALISLHLLYYFI"
     gene            3380..4003
                     /locus_tag="PXB27_001828"
     CDS             3380..4003
                     /locus_tag="PXB27_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HDL5346589.1"
                     /translation="MLTILFIHFAGLISPGPDFFYVVRQSASHSVKSGVLAATGISIG
                     IIFWAAFAIFGLAWLSNSIGSLFQYAIMIIGGCYLVYIGSQMVRVTENIIFVENKAQL
                     TPLNPWQEIKKGLLINIFNAKAGVYFTSVISAYVDNLSNTLQMFELLLLFVVSTFIYF
                     CVVALLFSRRPVRLFYAKYSRYIDNISGVIFILFGSMLIYEGFSHFI"
     gene            4115..4720
                     /gene="ruvA"
                     /locus_tag="PXB27_001829"
     CDS             4115..4720
                     /gene="ruvA"
                     /locus_tag="PXB27_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005686751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDL5346590.1"
                     /translation="MIGRLHGKIIEKQPPEILLDVQGVGYELLLPMTSFYSLPQIGEE
                     ATIFTHLVVREDAHLLFGFAQKQDRTLFRELIKTNGVGPKLALAILSAMSVSQFATAI
                     ENEELAKLTKIPGIGRKTAERLLVELKGKFKGMAQSDFFVEVSHEQIVASNTPDPANE
                     ALDALIALGYKPADAEKMIKKVNKSGATSEQLIREALKNSL"
     gene            4857..5861
                     /gene="ruvB"
                     /locus_tag="PXB27_001830"
     CDS             4857..5861
                     /gene="ruvB"
                     /locus_tag="PXB27_001830"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDL5346591.1"
                     /translation="MIEADRIISASPKREEEVIDRAIRPKLLADYVGQPSVHDQMEIF
                     IKAAKLREEALDHLLIFGPPGLGKTTLANIVANEMGVNIRTTSGPVLEKAGDLAAMLT
                     NLEPYDVLFIDEIHRLSPAIEEVLYPAMEDYQLDIMIGEGPAARSIKLDLPPFTLVGA
                     TTRAGSLTSPLRDRFGIVQRLEFYSVDDLTLIVKRSADCLNLNLSADGAYEVARRSRG
                     TPRIANRLLRRVRDYADVRNNGIITSDIAKQALAMLDVDSEGFDFMDIKLLQAIVERF
                     DGGPVGLDNLAAAIGEERDTIEDVLEPYLIQQGFLQRTPRGRIATSRTYAHLGITQID
                     "
     gene            complement(5960..6643)
                     /gene="artM"
                     /locus_tag="PXB27_001831"
     CDS             complement(5960..6643)
                     /gene="artM"
                     /locus_tag="PXB27_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HDL5346592.1"
                     /translation="MWWDYLVEISQGIGTSLQLTFVALIIAFILAIILTFILSLENRP
                     LKWLVNGFITLFTGTPLLIQFFLIYSGPGQFEWITESAAWVLLSDAWFCAMLALALNS
                     AAYSTLLFHGAVKAIPKGQWETCAALGLSRLDTLKILVPYALKRALPSYSNEIVLVFK
                     GTSLASTITIMDIMGYARQLYGTEYDALSIYGMAGVIYLVITGIMTIVLRKLESRVLA
                     FERFETEKA"
     gene            complement(6643..7314)
                     /gene="artQ"
                     /locus_tag="PXB27_001832"
     CDS             complement(6643..7314)
                     /gene="artQ"
                     /locus_tag="PXB27_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HDL5346593.1"
                     /translation="MSSDILNLLVNATLMTLGIALASLLIGMLLSIFFVVLETSKIAC
                     IRKPTSIIITLLRGLPEILVVFLIYFGLIQVIFLITDEFAELNAFWCGAIALGIIFAA
                     YASQSLRGAIKAIPVGQWESGTALGLSRAQVFVRIILPQVWRHALPGLSNQWLVLLKD
                     TALVSLIGVDDLMRQSELINANTHQPFTWYGIAALIYLAITLISQRVIKVLEGRFTRF
                     ERGVA"
     gene            complement(7314..8039)
                     /locus_tag="PXB27_001833"
     CDS             complement(7314..8039)
                     /locus_tag="PXB27_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDL5346594.1"
                     /translation="MKKLLLASAIAFATLSANAQETITFAMEPSYAPFETTNEKGEVI
                     GFDVDIAKALCEELKATCEFKTQSFDSLIAGLKFKNFDAIISGMDITEARQKQVAFTN
                     AYLENSVSYVAMKGKFTPETAKNVGVQNGTTFQQYLSKNAKQYNLKPYASLQSAVLDL
                     KNGRVDMVFGDTPVLAEWLKTEKELDFVGEKVVDKAFFGNGYGIAVNKSNTALLEKLN
                     SALATIKTNGKYEQIYNTWMAGK"
     gene            complement(8065..8799)
                     /gene="artP"
                     /locus_tag="PXB27_001834"
     CDS             complement(8065..8799)
                     /gene="artP"
                     /locus_tag="PXB27_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HDL5346595.1"
                     /translation="MAIRIQNVNFFYGSNQALFDINLEIEKGDTVVLLGPSGAGKSTL
                     IRTLNLMEVPQSGTLAIANHQFDLTAKTDSKAVSLLRREVGMVFQQYHLWPHLTVMQN
                     LIEAPMKVLGLSKEEAVARAKGHLARLRLSEFAERFPLHLSGGQQQRVAIARALMMQP
                     QVLLFDEPTAALDPEITAQVVDIIKELQSTGITQVIVTHEVGVARKVASKVVYMEKGT
                     IIEQGDASCFESPKTEQFAQYLSHSE"
BASE COUNT         2680 a         1885 c         1711 g         2616 t
ORIGIN      
        1 tgtaatatcc cttatgaaat taacatcaaa ttatagaggt tgatatgtgg cgaattttta
       61 tatatttctt atgcggtttt tcattagcct atgcgaatac catttccata gttttaaaaa
      121 atgctccaac ttcgcttatt tttacttatt tagcggaaga aaccggtaaa aatatggtgc
      181 ttgatgataa tattgaaacc aaatcaacac tacgattaga aaataaatcg gttgacgaga
      241 tttttaaaac gcttggcaaa gtcaataaat tatccttaac gcaagaagat gatattgttt
      301 atattcacaa aaaagaggaa aaagcggctg acttaacgcc tctgccaata atgaatttgc
      361 aaaataatgg gcaaaataca ccgcttgtca ccgcccctaa gttaatcaca aaaaccttga
      421 aactacatta tgcaaaggct tcagaggtga ttgagtcgtt aaccaaaggc agcggaacgt
      481 ttttatcgga aaacggttat attcactttg atgaaagaag caatagcctg attgtgaaag
      541 acagtgcaaa atcgcttaaa aatatcgaga atttagtaaa acagctcgac caacccaccg
      601 agcaaattgc gattgaggct agaattgtca ctatcagtag cgagcatttg caagagcttg
      661 gcgttcgctg gggaattttt tctcgtgggg ctgggcacta taaatttggc ggacggctgg
      721 aaggcaatgg actcaacaat gtcaccaata acttaaatgt aaattttccg attactggtg
      781 gagcatctgc cgtattacag gtttcgtcca ttaatagcag agtgcttgat ttagaattaa
      841 gtgcattaga acaagaaaat agtgtggaaa ttattgccag cccacgcctg ttaaccacca
      901 ataaaaagcc ggcaagtatc aagcaaggca cggaaattcc ttatgtgatg tacaacacca
      961 aatcggaagc caccgatgtc gaatttaaag aagcggtatt agggcttgaa gtcgttccac
     1021 acctttcaac ccaaaatcaa attctactcg atctgatcgt tacccaaaat tcgccaaatt
     1081 cccaatccgg cagtagtggc ttaattacga ttgataaaca agagctaaat acgcaagtat
     1141 ttgccaaaca cggcgaaacc attgtacttg gtggtatttt tcaacattta acccaaaaag
     1201 gcgaagataa agtgccgatt ttaggctcaa ttcccttcat caagcggtta tttagtcaaa
     1261 ctcgagataa aatcagcaaa agagagctag tcatttttgt gacgccttat atccttcaat
     1321 ctaatgaaaa gagcagcaac cgcaaaaaat caaaatagca catcataaac gcaagcaaaa
     1381 ataggggaag tgtgatagaa tcagccgtca ttttttgagt taaagattta gaggttttat
     1441 gaaaatttcc ccacgtcgcc gtgcaagaga atgtgcggtg caagccattt attcttggta
     1501 tatttcccaa aacacggttg aagaagtgga attggcgttt gtgaccgatc aagatatgaa
     1561 aggcgttgat atgccttatt tccgtaaact tttccgtggc gttgtggata atgtagaagc
     1621 cattgatgaa gcattacgcc cttatttaga tcgtaaagag tcagatgttg atcctattga
     1681 acgttctatt ttgcgtttat caacttacga attaaaattt gaacacgatg tgcctttccg
     1741 tgtagtcatc aatgaaggga ttgaagtcgc taaagtgttc ggctctgatg acagccacaa
     1801 atacattaac ggcattttag ataaattagc cccagcatta ggcagaaaat aataagattc
     1861 cccaatttat acataaattg gggattttga ttataacgct caaggagatt ttttatgggt
     1921 gaatttgata ttatcgaacg ctattttaat gcttctcaac gcccacctcg caaagatgtt
     1981 ttactttcta tcggcgatga ctgtgcgctc acatcactca aaccaaatca gcaacttgcg
     2041 atcacaaccg atacccttgt ttgcggcact cattttctac cgactatttc acctgccgat
     2101 ctcgcttata aaagtgtggc gtccaattta agcgatcttg ccgcaatggg tgcagaacct
     2161 gcatggatct ctttagcctt aaccttacct gaaattaatc acgattggct agctgaattt
     2221 agtgaacatt tctttgagat tcttgatcac tataatgttg atttgattgg aggggatact
     2281 acttgtggac cactatcttt gacaatcaca gctcaaggga ttgtgccaca agatagaggc
     2341 ttattccgcc ataatgctaa agtaggcgat tggatctatg tttccggcac actcggtgac
     2401 agtgcaggtg gtttgcaact tctgctaaat cagcaaagcg agcaagtgaa ttggaataaa
     2461 gagcaaagct acctgattca acgccattta cgcccaacac ctcgagtttt acttggcttg
     2521 actatcgcct ctattgctaa tgcagcgatt gatatttcag atggtctatt gtctgattta
     2581 agccatattt taaagcgcag tgactgtggt gcagtattaa accttgagaa tttccccttg
     2641 tctgacgtct tacttagttg ttattctcga acagaggcag aatattttgc cttaaacggt
     2701 ggcgaagatt acgaactctg ctttaccgtg ccaaatgaca acaaagccaa attagacaaa
     2761 gcattagcca atatcggagt aacttataca tgtattgggc aaattcgttc ggctaaagaa
     2821 ggtttaaccc tattacgtca taatcagcct gtcaccttgc cctcacaacg aggctacgat
     2881 cattttaaaa aataacgcaa tgaaaccaat taatcttaaa aatccgattc atttttttgc
     2941 ctttggcttt ggttcaggct tattaaagcc tgctcccggc acctggggta gcctcgccgg
     3001 gctaattgta gcaatagcct tgtggtctat cacacaatcc gccctgtttt tcggcttact
     3061 caccctagtt tcttttattg ccggttgtgt tatttgcacc aaagccagcc aagatctaga
     3121 agtacacgat gacgggcgaa ttgtttggga cgaaatagtt gccatctttt tgatgttcac
     3181 ctgtttgcct gaatacaatc ttcttggcta cctgctcgcc tttatcagct ttcgcatttt
     3241 tgatattttg aagccttttc caatccgcta ttttgatgaa aaattagaaa gcggtttagg
     3301 cattatgttt gatgatattc tcgcagccat ttttgccctg atttcacttc atcttttata
     3361 ttatttcatt taaaaattta tgctcactat tttatttatt cattttgccg ggcttatcag
     3421 ccccggcccc gatttcttct atgtcgtcag acaatccgcc agccactcag ttaaaagtgg
     3481 ggtacttgcc gcaaccggaa tctcaatcgg cattattttt tgggcagcct ttgcgatttt
     3541 tggtttagcg tggctcagta atagtattgg ctcgctgttt caatatgcca ttatgattat
     3601 cggtggctgt tacctggttt atattggctc tcaaatggta cgggtaaccg aaaatatcat
     3661 ttttgttgaa aataaagctc aactcacgcc gctaaaccct tggcaggaaa ttaaaaaagg
     3721 cttattaatc aatatcttta atgccaaagc tggcgtttac tttaccagcg tcatttcagc
     3781 ctatgtagat aatttaagca ataccctaca aatgttcgaa ctacttctac tctttgtggt
     3841 aagtaccttt atttatttct gtgtggtggc actactcttt tctcgccgac cggtcagact
     3901 gttctatgcc aaatatagtc gctatattga caatatctca ggcgtaattt tcatcttatt
     3961 cggctcgatg ctgatttatg aaggattcag ccactttatc taatcacaag cggtctgatt
     4021 tcactaaaaa tttgcaaatt ccgctatcaa taaatagtgt tattttatac agtttctgct
     4081 atgctactgc caattttaac tctgagagaa caaaatgatt ggacgattac acggcaaaat
     4141 tattgaaaag caaccgcctg aaattttatt agacgtgcaa ggggtaggct atgaactact
     4201 gctcccgatg accagtttct acagcctgcc acaaattggc gaagaggcga ctattttcac
     4261 ccatttagtg gtgcgagaag atgctcactt gctattcggc tttgcccaaa aacaagatag
     4321 aactctattc cgtgaattaa ttaaaaccaa cggtgtcggg ccgaagctcg cgttggcaat
     4381 tctatcagca atgtcggtgt cgcaatttgc caccgcgatt gaaaatgaag agctggcaaa
     4441 attaaccaaa attccgggaa tcggtcggaa aaccgcagaa cgcttattgg tggaactcaa
     4501 aggcaaattt aaaggaatgg cacagagtga tttctttgtg gaagtatcgc acgagcaaat
     4561 tgtggcaagt aatacgccag acccggcaaa tgaagccctt gatgccttaa tagccctagg
     4621 ctacaaacct gccgatgcgg aaaaaatgat taagaaagtg aataaatccg gtgcaacgag
     4681 cgaacagctt attcgtgagg cgttaaaaaa ttcattatga tattgatagt atagggcgaa
     4741 agatatttcg cccctacaat ctcattttta aaaatatatg tgtaggggca aatcaatatt
     4801 tgccctcatg agtaatatag ggtaatacca ttccactaca acaataatct aaatgtatga
     4861 ttgaagcaga tagaatcatc agtgcctcgc ctaagcgtga ggaagaagtt atagaccgag
     4921 caatccgccc gaaactgctt gccgactatg tcgggcagcc ttctgtgcat gatcaaatgg
     4981 agatcttcat caaagccgct aaattgcggg aagaggcatt agatcacttg ctcattttcg
     5041 gcccgcccgg tttggggaaa actacccttg ccaacattgt ggcaaacgaa atgggggtga
     5101 atattcgcac aacttcaggg ccggtgctgg aaaaagcagg cgatttggca gcaatgctga
     5161 ccaaccttga accttacgat gtgctgttta ttgatgagat tcaccgcctt tctccagcga
     5221 ttgaagaagt gctttatcct gcaatggaag attaccagct cgatattatg attggggaag
     5281 gacctgctgc tcgttccatc aagctcgatc taccgccttt tacccttgta ggggcaacca
     5341 cccgtgccgg ctcactgacc tcaccgttac gagatcgttt tgggattgta caacgcttag
     5401 aattttactc agtggacgat cttactctga ttgtcaaacg cagcgccgat tgcttaaatc
     5461 tcaatttgtc ggcagacggt gcttatgaag tggctcgccg ctcccgtggc acaccccgta
     5521 ttgccaaccg cttactccgc cgagttcggg attacgccga tgtgcgtaat aatggcatta
     5581 ttacctcaga cattgccaaa caagctctgg caatgctcga tgtggattcg gaaggctttg
     5641 attttatgga cattaaactg ttgcaagcga ttgtagaacg ctttgatggc gggccagttg
     5701 gattagacaa tcttgcggca gctatcggcg aagaacgaga tacgattgaa gatgtgctag
     5761 agccttatct gattcagcaa ggctttctac aacgcacacc aagaggcaga attgctacca
     5821 gcagaaccta tgcccattta ggtataaccc aaatagatta gtcatatttc taaaggcatt
     5881 gatacataaa taaaaagcgg atagttagta ctatccgctt tcatttgcaa aaaatcaata
     5941 aaatttgacc gcttatccgc taggcttttt cagtctcaaa acgctcaaaa gctaatactc
     6001 tgctttctaa ttttctgaga acgattgtca taatgccggt aattaccaag taaataaccc
     6061 ctgccatacc ataaattgat aatgcgtcat actctgtacc ataaagctgg cgagcatagc
     6121 ccataatatc cataatcgta atggttgaag caagcgatgt gcctttaaaa actagcacaa
     6181 tctcattact ataagaaggt aaagcacgtt ttaatgcgta aggcactaaa attttcagtg
     6241 tatctaaacg gctcaaacca agggctgcgc aagtttccca ttgccccttc ggaatcgctt
     6301 ttaccgcacc gtggaacaac aatgtcgaat aggcagcact gtttaacgct aaagccaaca
     6361 ttgcacagaa ccaagcgtcg gataataata cccaagccgc actttctgta atccattcaa
     6421 attgccccgg gccactataa atcaagaaaa attgaattaa taaaggcgtt ccggtaaaaa
     6481 gcgtaataaa tccgtttacc aaccacttta acggacgatt ttctaacgag agaataaacg
     6541 taagaatgat cgctaaaata aaggcaatga tgagcgccac aaaagtgagc tgtaggctgg
     6601 tgccaatacc ctgtgaaatt tcaactaaat aatcccacca cattaagcaa ctcctcgctc
     6661 aaaacgggta aatctacctt ctaatacttt aatcactcgc tggctaatta gagtaatcgc
     6721 taaataaata agtgctgcta tcccatacca agtaaaaggt tgatgagtat tagcattaat
     6781 taactccgat tgacgcatta aatcatctac cccaattaac gataccaatg ccgtatcttt
     6841 taacagcact aaccactgat tgcttaagcc tggtaaagcg tgtcgccaga cttgagggag
     6901 aataatgcga acaaatactt gcgctcggct taaccctaac gccgtaccag attcccactg
     6961 cccaactgga atagctttaa ttgcaccacg taaagattgg gaagcataag cagcaaaaat
     7021 aatacccagc gcaatagccc cacaccagaa agcatttaac tccgcaaact catcggtgat
     7081 caagaaaata acttggataa gaccaaaata gatcaaaaat accactaaaa tctccggcaa
     7141 cccacgcaat aatgtaatta ttatactagt tggtttacgg atacatgcaa tcttactggt
     7201 ttcaagtacc acaaaaaaga ttgacaataa cattccaatc aagaggcttg ccagcgcaat
     7261 ccctaaggtc atcagggttg cgttgacaag taagtttaaa atatcgctag acattatttc
     7321 ccagccatcc aagtattata aatttgctca tatttaccat ttgttttgat ggtagctaaa
     7381 gccgaattta atttttcaag caatgcagtg ttagatttat ttaccgcaat accatatcca
     7441 ttaccaaaaa aggctttatc gactactttt tcacccacga aatctaactc tttttcggtt
     7501 tttagccact ctgccaatac tggtgtatca ccaaatacca tatcaacacg tccatttttt
     7561 aaatctaaca ctgctgattg taggctagcg taaggtttta aattatattg tttggcattt
     7621 ttgcttaagt attgttgaaa agttgtacca ttttgcacgc cgacattctt agctgtttcc
     7681 ggtgtaaatt tccccttcat tgcgacataa cttactgagt tttctaaata agcattggta
     7741 aaggcaacct gtttttgacg cgcttcagta atatccatgc ctgaaataat tgcatcaaaa
     7801 tttttaaatt ttaatcccgc aattaagcta tcgaatgatt gcgttttaaa ttcgcaagtt
     7861 gcttttagtt cttcacataa tgcttttgca atatctacat caaagccaat aacttcacct
     7921 ttttcatttg ttgtttcaaa aggggcgtaa gaaggctcca tcgcaaaagt gatagtttct
     7981 tgtgcattgg cagaaagcgt tgcaaatgcg atagcggaag ctaataataa ttttttcatt
     8041 gttgtgtcct tttgttgtta tagattactc tgagtgagat aaatattgag caaattgctc
     8101 tgttttcggt gattcaaaac aacttgcatc accttgttca ataattgtac ctttttccat
     8161 gtacaccact ttgcttgcca ccttacgagc aacgcctact tcgtgagtca caatcacctg
     8221 agtaatgcct gtagattgca attctttaat aatatcaact acttgtgcag taatttccgg
     8281 gtcaagtgct gcggtaggtt catcaaataa taagacctgt ggctgcatca tcagtgctct
     8341 ggcaatcgcc acccgttgtt gctgaccacc tgaaagatgc agtgggaaac gctcggcaaa
     8401 ctcgctcaaa cgcaaacgtg ccaaatgtcc ttttgctctg gcaaccgctt cttctttgct
     8461 taacccaagc actttcatcg gggcttcaat taagttttgc attacggtta aatgcggcca
     8521 taaatgatac tgctggaaca ccattcccac ctcacggcgt aacaaactca ccgctttgct
     8581 gtcggtttta gcagttaaat caaattgatg atttgcaatc gccaaagtgc ctgattgcgg
     8641 tacttccatt aagtttaagg ttcgaattag ggtactttta cctgccccac tcgggccaag
     8701 caatacaaca gtatcccctt tttcaatctc taagttaatg tcaaataagg cttggtttga
     8761 gccataaaaa aaattgacat tttgaatacg aattgccatt cgttaaaatc ctactacatt
     8821 taaaataatg aatgaatact aattattttt gcataactat gcaatatttt tttcttttag
     8881 aacaaaattt tt
//
LOCUS       DANLUQ010000106         8856 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.107, whole genome shotgun sequence.
ACCESSION   DANLUQ010000106 DANLUQ010000000
VERSION     DANLUQ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8856
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.107"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..619
                     /locus_tag="PXB27_001835"
     CDS             <1..619
                     /locus_tag="PXB27_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDL5346596.1"
                     /translation="PHAILESNRIDKGNRKFIAVQYPEEIDIKSKNGKVAKQFCQRKN
                     LPLYITEISKERIRRAGKQILQNNPENRPLDVGFKVFKLTESHFKQWQSPTSENLADQ
                     LEMFIDPVSEKADTQAVLYELLLRLGLKLTAKVRLENKVFWVEENGLIFALLLNAADE
                     EIIQTVIAQQPKKVIALDRLFNGNDARKKNTELQMQDAGITFFVI"
     gene            629..3265
                     /locus_tag="PXB27_001836"
     CDS             629..3265
                     /locus_tag="PXB27_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL5346597.1"
                     /translation="MELQFETLEYQLQAVNATVNLFVGQPNAATEFSLKAQNDMRFVP
                     NLGLQISDEQLQQNLANLQNRQKIDRTLLVEQGKNFTVEMETGTGKTYVYLRTIFELN
                     RQYGWQKFVIVVPSVAIREGVLHTLETTKSHFNTVFDNPSVNQKFEYKSNQTSRLKSF
                     ASANHIEILVMNIDAFTKESNVINTVNESGDAPIFYIQQANPIVIIDEPQNMETEIRR
                     NAIESLSPLFTLRYSATHKNAYNPIFSLNPVQAYEMGLVKQIEVDSVLAENEVNGAYL
                     SLKEIKTGAKSWSAKIEILFNDKKAMKKKVISVKPNQDLYELSNQNEMYRSGFILSGM
                     DTDSQMVSFSNGTQIFQGAEHSQLKEDVQKMQIRRTIGEHLQKEKKLRPLGIKVLSLF
                     FIDKVDNYRPSAQSPTGGKFAKWFEEIYLELAKEEPQGVHNGYFSQDKKGVAKDTNGT
                     TQADNETYQLIMRDKEKLLSLENPLRFIFSHSALKEGWDNPNVFQICTLNDTASEMKK
                     RQEIGRGLRLPVNQQGLRIYDRHLNTLTIIANESYEQFAATLQREIEEDCGVQFAKSN
                     VKKREDKKAVRYRKDFSLHPEFAAIWERIKHQTTYRVDFSNAELIESAVEKIRKMPDI
                     EATKIKHSKVKLEMNETGISTNYRSSNETAVNTQWAIPDLLSEIQKKTGLTRATLCKI
                     LQKSDRLQEVFRNPQRFIDLIAEKINLALQQIMTDGVEYHKIAGKTYEMTLFGDFDFY
                     LNQYTFEVQDKTKTIYEDYIALDSQTENIFARHCESSEQVEFYFKLPKRFKIPTPLGS
                     YNPDWAVVFKGENKIYLVAETKNTGKGIQAGVDEEKLDESERMKIDYARKHFKGLQRD
                     YKDLDYRVVQKVEELSAEYCKI"
     gene            3397..5829
                     /gene="gyrB"
                     /locus_tag="PXB27_001837"
     CDS             3397..5829
                     /gene="gyrB"
                     /locus_tag="PXB27_001837"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDL5346598.1"
                     /translation="MSEQTQATSYDSSSIKVLRGLDAVRKRPGMYIGDTDDGTGLHHM
                     VFEVVDNAIDEALAGHCKDIIVTIHSDNSVSVQDDGRGIPVGIHPEEGVSAAEVIMTV
                     LHAGGKFDDNSYKVSGGLHGVGVSVVNALSSKLQLTIRREGHVHEQFYSLGEPDAPLA
                     IIGNTEKTGTSVRFWPSLDIFKNKTEFEYKILSKRLRELSFLNSGVSIKLIDERDGKE
                     EHFKYEGGIKAYVEYLNEGKTHIHNTPFYLTTEKDGIGVEISLQWNDSYNENVYCFTN
                     NIPQRDGGTHLAGFRGALTRALKNYMDNSGVLKKADANIDASGDDAREGLVAVISVKV
                     PDPKFSSQTKDKLVSSEVRGAVESSMNEALSDYLAENPDDAKNIVTKIIDAARAREAA
                     RKAREMTRRKGALDLGGLPGKLADCQEKDPALSELYLVEGDSAGGSAKQGRDRKNQAI
                     LPLKGKILNVEKARFDKMLSSQEVATLITALGTGIGRDDYNLEKLRYHKIIIMTDADV
                     DGAHIRTLLLTFFYRQMPELIENGYIYIAQPPLYKVKKGKQERYIQDNDEMSQYEIDI
                     ALEGAGLYVSEDAPALSGVALENLIAQYNGVQKLIERLSRRYPTLLLNELIYSKPLSV
                     EFAKNQANMTAWSEQFVATLMAKEVGGSFYRINTLFNEERQIYEPEIIVTTHGMDSTY
                     RLDYNFINGNEYARIVALGNELNDLLSDSAYVVRGERRQEISSFAEALDWLVKESRKG
                     LTIQRYKGLGEMNPEQLWETTMDPVARKMLQVNITDAIAADKLFSTLMGDEVEPRRDF
                     IETNALYAQLDI"
     gene            complement(5882..6844)
                     /gene="cmoB"
                     /locus_tag="PXB27_001838"
     CDS             complement(5882..6844)
                     /gene="cmoB"
                     /locus_tag="PXB27_001838"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005707797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HDL5346599.1"
                     /translation="MIDFRPFYQQIATTDLSAWLETLPLQLKQWEKTTHADYAKWAKV
                     ADFLPNSTACINLKDKVESISSKPLSEGEVKQITHHLKQLMPWRKGPYHLHGIHIDTE
                     WRSDFKWDRVLPHLAPLKDRTILDVGCGSGYHMWRMVGEGAKMVVGIDPTELFLCQFE
                     AVRKLLGNDRRANLIPLGIEQMQPLAAFDTVFSMGVLYHRKSPLDHLSQLKAQLVRGG
                     ELVLETLVIDGDINDVLVPADRYAKMKNIYFIPSVAALINWLEKVGFKNVRCVDEAIT
                     TLEEQRKTDWLENESLVDFLDPNDHSKTIEGYPAPKRAVILANA"
     gene            complement(6941..8704)
                     /locus_tag="PXB27_001839"
     CDS             complement(6941..8704)
                     /locus_tag="PXB27_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HDL5346600.1"
                     /translation="MSEQTFITGKDAALEDSIATFQHKLKTLGFNIEEASWLNPVPNV
                     WSVHIRDADCPQCFSNGKGASKKAALASALGEYFERLSTNYFWADFYLGQEIANSEFV
                     HYPTEKWFPIDDEAIPPMEMMDEFLWEYFDPNGEITPELLVDLQSGNYDRGIVTLPYV
                     RQSDNETVYIPQSIIGNLFVSNGMSAGNTKNEARVQGLSEVFERFVKNRIITEAISLP
                     EIPQSVIDGYPTIKASIEKLEQEDFPILCYDASLGGEFPVICVILLNPNNGTCFASFG
                     AHPNFQVAFERTVTELLQGRSLKDLDVFAPPSFNNEDVADHANLETHFIDSSGLISWD
                     LFKRDADYPFVHWDFSGTTEQEFNNMMAIFNKHNQPVYIMDYEHLGVYACRILAPGMS
                     NIYPSDDLVYANNNMGMDWREILLDLPHFHHDAETYQELLDELDEQSIDDLTRVREFI
                     GVVAEKGSAMQTLRIGELKSMLHLALGNLEQASDWANWTANMNASVFSAERNNYYRCL
                     IQSIELFLDEERDSEQYRMVFEKMYGKQAVEAAWGAIQGGNPFHGLTASDESLENMTA
                     HQKLLNAYRKVQKAKVNPIAR"
BASE COUNT         2818 a         1709 c         1888 g         2441 t
ORIGIN      
        1 tccacacgca atattggaaa gtaatagaat tgataagggt aatagaaaat ttattgcggt
       61 tcaatatcct gaagaaattg atatcaagag taagaatgga aaagtagcaa agcaattttg
      121 ccaaagaaaa aatttaccac tatatataac tgaaatttca aaagaacgta ttcgccgtgc
      181 cggaaagcag attttgcaaa ataaccccga aaaccgaccg cttgatgtag gttttaaagt
      241 cttcaaactg acagaaagcc attttaaaca atggcaatcg cccacatctg aaaatttagc
      301 cgatcaactt gaaatgttta ttgatcctgt tagtgaaaaa gcggatacgc aagccgtttt
      361 atatgagctt ttattgcgtt tagggttaaa actcacggca aaagtgcggt tggaaaataa
      421 ggtgttttgg gtggaagaaa acgggctgat tttcgccttg ctgttaaatg cagccgacga
      481 agaaattatt caaactgtta tcgctcaaca accgaaaaaa gtcatcgctt tagaccgttt
      541 attcaacggc aatgatgccc ggaagaaaaa caccgaattg caaatgcaag atgccggcat
      601 tacgtttttt gtgatttaag gagaaaaaat ggaactccaa tttgaaacct tagagtatca
      661 attacaagcc gttaatgcca cagtaaattt gtttgtcgga cagccgaatg ccgcaactga
      721 attttcactc aaagcccaaa atgatatgcg gtttgtgccg aatttaggct tgcagattag
      781 tgatgagcag ttacaacaga atttggctaa tctgcaaaac aggcaaaaaa tcgaccgcac
      841 tttgcttgtc gaacagggaa aaaattttac cgtggaaatg gaaactggta cagggaaaac
      901 ctatgtctat ttacgcacga tttttgaatt aaaccgtcaa tacggctggc aaaaatttgt
      961 gattgtcgtg ccgagtgtgg caattcgtga gggagtgttg catacgttgg aaactacaaa
     1021 aagccatttt aatacggtat ttgataaccc gagcgtcaat caaaaatttg aatataaaag
     1081 taaccaaact tcacgcttaa aatcctttgc tagtgctaat catattgaaa ttttagtgat
     1141 gaatattgat gctttcacca aagaaagtaa cgtgattaat accgtgaatg aaagcggtga
     1201 tgctccgatt ttctatattc aacaggcgaa tccgattgtg attattgatg aaccgcaaaa
     1261 tatggaaacg gagattcgcc gtaatgcgat tgagagttta agtccgcttt tcactttgcg
     1321 ttattctgcc acccataaaa atgcgtataa cccgattttt agcctcaatc cggtgcaagc
     1381 ctatgaaatg ggattagtta aacaaattga ggtagatagc gtattggcgg aaaatgaggt
     1441 aaatggagct tatctttccc tcaaagagat taaaacaggg gcaaaaagct ggtcggcaaa
     1501 aatcgaaatt ttatttaacg ataaaaaagc gatgaaaaag aaagtgatta gcgtgaaacc
     1561 gaatcaggat ttgtatgagc tttccaacca aaatgaaatg tatcgttcag ggtttatatt
     1621 aagcggaatg gataccgata gccaaatggt tagcttctcc aacggcacac aaatttttca
     1681 aggggcggaa cattcccaac ttaaagaaga tgtgcaaaaa atgcagattc gccgcactat
     1741 tggcgagcat ttgcaaaaag aaaagaaatt acgaccgctt gggatcaagg tgttgtcttt
     1801 gttctttatc gataaagtgg ataattatcg cccttctgca caaagcccta cggggggaaa
     1861 atttgcaaaa tggtttgagg aaatttattt ggagctagcc aaagaagagc ctcaaggcgt
     1921 gcataacggt tatttctcac aggataaaaa aggcgtagca aaagatacta atggcacaac
     1981 gcaagcggat aacgagactt atcaactgat tatgcgggat aaagaaaaat tgctttcgct
     2041 ggagaatccg ttacggttta ttttctcgca ttctgcctta aaagaaggat gggataatcc
     2101 gaatgtgttt caaatttgta ctttaaacga tactgcaagc gaaatgaaaa aacgccaaga
     2161 gattgggcgg ggcttgcgtt tgcctgtgaa tcaacaagga ttgcggattt atgatcgtca
     2221 tctaaatacg ctgacaatta tcgcaaatga aagttacgaa caatttgccg ccacattaca
     2281 gcgtgaaatt gaggaagatt gtggtgtaca gtttgccaaa agcaatgtta aaaaacggga
     2341 agataaaaaa gccgttcgct accgtaaaga tttcagctta catccggaat ttgcggcaat
     2401 ttgggaacgg attaaacacc aaaccactta tcgggtagat ttcagtaatg cggaattgat
     2461 tgaaagtgcg gtggaaaaaa tccgcaaaat gcccgatatc gaggcgacaa aaattaagca
     2521 ttccaaagtg aaattggaaa tgaatgaaac agggatttca actaattatc gctcatctaa
     2581 tgaaacagcg gtaaatacac aatgggcgat acctgattta ttgagtgaga ttcaaaagaa
     2641 aaccgggctg acgcgtgcga ccctttgcaa aattttacaa aaatccgacc gcttgcaaga
     2701 agtcttccgc aatcctcaac gctttattga tttaattgca gagaaaatca accttgcttt
     2761 acaacaaatt atgacagacg gggtggaata tcacaaaatt gccggaaaaa cctatgaaat
     2821 gacgttattc ggcgattttg atttttacct caatcaatat acttttgaag ttcaagataa
     2881 aacaaaaacg atttatgaag actatattgc actggattcc caaacagaaa atatcttcgc
     2941 ccgacattgt gaatctagtg agcaagtgga gttttatttt aaattaccca aacgttttaa
     3001 aattccaacg ccattaggca gctataatcc cgattgggca gttgtgttta agggagaaaa
     3061 taaaatttac ttggttgccg aaacgaaaaa tacaggcaaa ggtattcaag caggagtaga
     3121 tgaagaaaaa ttagatgaaa gtgaacgaat gaaaatcgac tatgcccgta agcatttcaa
     3181 aggcttacag agagattata aggatttgga ttatagggtt gtacagaaag tggaggagct
     3241 gtctgcggag tattgtaaaa tttaatctgt ggtcttgtag aattgagata gactgcaatt
     3301 tcaactaaaa tttaaccgct tgttttgcga tacttttacg catttttctg ctataatcgg
     3361 cacaattttt tcgatttcaa attagtagga ataagaatgt ctgaacaaac tcaagcaaca
     3421 agttatgatt cttccagtat taaggtgtta cgcggcttag atgcggtgcg taaacgcccg
     3481 ggaatgtata tcggcgatac cgatgacggt acgggcttac accatatggt gtttgaggtg
     3541 gttgataatg cgattgacga agcattagcc gggcattgta aagacattat tgtcacaatt
     3601 cattccgata attcggtttc tgtgcaagat gacggtcgtg gaattccggt ggggattcac
     3661 ccggaagaag gcgtttcggc agcagaagtc attatgactg ttcttcacgc aggcggtaaa
     3721 tttgacgata actcctataa ggtttccggc ggtttgcacg gtgtgggcgt atcggtggtg
     3781 aatgcacttt cctctaaatt acaactcact attcgccgtg aaggacacgt tcacgagcaa
     3841 ttctatagct taggtgagcc tgatgcacct ttagcgatta tcggcaatac cgaaaaaacg
     3901 gggacgtcgg ttcgtttctg gccaagtttg gatattttca aaaataaaac cgaatttgaa
     3961 tataaaattc tgtctaaacg cttgcgtgaa ctatcatttt tgaattcagg cgtttcgatt
     4021 aaattaattg atgaacgaga cggcaaagaa gagcatttta aatatgaggg cggtattaaa
     4081 gcctatgttg agtatttaaa cgaaggcaaa actcatattc acaatacgcc attctatttg
     4141 accactgaga aagacggcat tggcgtggaa atttccctac agtggaatga cagttacaac
     4201 gaaaatgtgt actgctttac caataatatt ccacagcgtg atggcggtac gcacttagcc
     4261 ggtttccgtg gggcattaac tcgtgcgttg aaaaactata tggataacag cggggtattg
     4321 aaaaaagccg atgcgaacat tgatgcctca ggtgatgatg cccgtgaagg tttagtagcg
     4381 gtgatttcgg tgaaagtgcc ggatccgaaa ttctcctcac aaaccaaaga taaattggtg
     4441 tcgtcagaag tgcgtggagc agtggaaagc tcaatgaatg aggctttatc cgactattta
     4501 gccgaaaatc cggacgatgc aaaaaatatc gttaccaaaa ttattgatgc agcccgtgca
     4561 agagaagcag ctcgtaaagc ccgtgaaatg acccgccgta aaggggcgtt agatttaggc
     4621 ggcttaccgg gtaaattagc tgattgtcag gaaaaagatc cggcgttatc ggagctttac
     4681 ttggtggagg gtgactctgc cggtggctcg gcaaaacaag ggcgtgaccg taaaaaccaa
     4741 gcgattttgc cgttaaaagg gaaaattctg aacgtggaaa aagcccgctt tgataaaatg
     4801 ctttcttcac aagaagtagc aaccttgatt accgctttag gcacaggcat cgggcgtgat
     4861 gattataatt tggaaaaatt acgttaccac aaaatcatta ttatgaccga tgcggacgtg
     4921 gatggtgcac atattcgtac gcttttatta actttcttct accgtcaaat gccggagctg
     4981 attgaaaacg gctatattta tattgcacag ccaccgcttt ataaagtgaa aaaaggcaag
     5041 caagagcgtt atattcaaga taacgatgaa atgtcgcaat atgaaattga tattgcatta
     5101 gaaggtgcag gcttatatgt gagtgaagat gctccagcgt taagtggcgt agcgttagag
     5161 aacttaattg cccaatataa cggcgtacaa aaattaattg aacgtttaag ccgtcgttac
     5221 ccaacattat tacttaacga attaatttat tcaaaaccgc tttcggtgga atttgcaaaa
     5281 aatcaagcaa atatgaccgc ttggagcgag caatttgtcg ctacattgat ggcaaaagag
     5341 gtgggcggca gtttctaccg tattaatacc ttgttcaatg aagaacgcca aatttatgag
     5401 ccggaaatta ttgtgactac acacggtatg gacagtacct atcgtttaga ttacaacttt
     5461 atcaacggta atgaatacgc tcgcattgtg gcattaggca acgagctgaa cgatttacta
     5521 tctgattcag cctatgtggt gcgtggtgaa cgtcgccaag aaatcagcag ttttgctgaa
     5581 gccttagatt ggttggtgaa agaatctcgt aaaggcttaa ctattcaacg ttataaaggg
     5641 ttaggtgaga tgaatccgga acaattatgg gaaaccacga tggatccggt ggctcgtaaa
     5701 atgctacagg tcaatattac cgatgcgatt gcggcagata aactcttcag tacgctgatg
     5761 ggcgatgaag ttgaaccacg ccgagatttc attgaaacta acgctttgta tgctcagtta
     5821 gatatttagt taaaaaatcc gcatttgaca tgcggatttt ttattctctt aaactagcat
     5881 tttaagcatt cgctaaaatc accgcccgct tcggagcagg gtagccttca atggttttgc
     5941 tatgatcgtt tggatctaaa aaatccacta aactttcatt ttccaaccaa tcggtttttc
     6001 gctgctcttc tagtgtagtg attgcctcat caacacaacg cacattttta aagccgactt
     6061 tttccagcca attaatgagt gcagcaacag aaggaataaa atagatattt ttcattttcg
     6121 catagcgatc agccggcact aaaacatcat taatatcgcc atcaatcacc agcgtttcta
     6181 acaccaattc accgcctctg actaattggg ctttgagttg agaaagatga tcaagcggtg
     6241 atttgcgatg ataaagcacc cccattgaaa acacggtatc aaaagcagca agcggttgca
     6301 tttgctcaat accaagtgga atcagattag cccgtctatc attccctaag agtttccgca
     6361 ctgcctcaaa ttggcacagg aacagctcgg tcgggtcaat accaactacc atttttgccc
     6421 cttcgcctac cattcgccac atatgataac cgctaccgca gccgacatct aaaatggtgc
     6481 ggtcttttaa cggtgcaaga tgcggaagca cacgatccca tttaaaatcg gaacgccatt
     6541 cggtatcaat atgaatcccg tgtagatgat aagggccttt acgccacggc attagttgct
     6601 ttaaatggtg ggtaatttgc ttgacttccc cctcagataa aggcttgctt gaaatagact
     6661 caaccttgtc ttttagattg atacaagcgg tcgaatttgg taaaaaatct gcaacttttg
     6721 cccacttggc atagtcagcg tgggtggtct tttcccattg tttgagttgt aagggtaagg
     6781 tttctaacca agcagaaagg tcggttgtgg caatttgttg ataaaaagga cgaaagtcaa
     6841 tcattttgaa atttatgtct aggggagaga cctatgccct cccgaaaggt agtaaataga
     6901 aaggacaggc acagaaccta cccctacgaa aaatataaaa ttatctcgca atcggattta
     6961 ctttcgcttt ttgcactttg cgataagcgt tcagtaattt ctgatgtgca gtcatatttt
     7021 caagcgattc gtccgatgca gttaaaccgt ggaacgggtt accgccttgg attgcacccc
     7081 aagcagcttc aaccgcttgt ttaccgtaca ttttctcaaa caccatacga tattgttccg
     7141 aatcacgctc ttcatctaag aacaactcaa tcgattggat taagcagcgg taataattgt
     7201 tacgttctgc tgagaatacg cttgcattca tattcgctgt ccagtttgcc cagtctgagg
     7261 cttgctctaa gttacctaaa gctaaatgca gcatcgattt aagctcaccg atacgcaatg
     7321 tttgcattgc agagcctttc tctgccacca cgccaataaa ttcacgcaca cgggttaaat
     7381 catcaatgct ttgctcatct aattcatcaa gcagttcttg gtaagtttca gcatcgtgat
     7441 ggaaatgtgg caaatctaac aagatttcac gccaatccat acccatattg ttattggcat
     7501 agaccaaatc atcgcttgga tagatattcg acatacccgg tgcaagaata cggcaagcgt
     7561 acacacctaa atgctcataa tccataatat acacaggctg attatgcttg ttgaaaatcg
     7621 ccatcatatt attaaattct tgctccgttg tgccggagaa gtcccaatgc acgaacggat
     7681 aatccgcatc acgtttgaaa agatcccacg aaattagacc gcttgaatca ataaagtgtg
     7741 tctcaaggtt cgcatgatct gctacatctt cattattaaa tgaaggtggg gcaaacacat
     7801 ctaaatcctt taagctacgg ccttgtaata actcggttac ggtacgttca aacgccactt
     7861 ggaaattcgg gtgtgcacca aaagaggcaa aacaagtacc gttatttggg tttaacaaga
     7921 tcacacaaat aactggaaat tctccgccta atgaagcgtc atagcataaa atcgggaagt
     7981 cttcttgctc tagtttctcg atagaggctt tgattgtcgg ataaccgtca atcacacttt
     8041 gcggaatttc cggcaagctg attgcctcag taataatacg gtttttgaca aaacgctcga
     8101 acacttcgga taagccctgc actcgagctt cattttttgt gttgcctgcc gacataccgt
     8161 ttgaaacaaa caggttgccg ataatgcttt gcggaatgta aaccgtttca ttgtcagatt
     8221 ggcgaacgta aggtagggta acaattccac gatcataatt tcctgattgt aaatcaacca
     8281 ataattccgg cgtgatttca ccattcggat caaaatattc ccataaaaac tcgtccatca
     8341 tctccattgg tggaatggct tcatcatcaa tcggaaacca tttttcggtt ggataatgca
     8401 caaattcact attagcaatc tcttgaccta aataaaaatc cgcccagaaa tagttggtag
     8461 ataaacgctc aaaatactca cctaatgcag aggcaagagc ggcttttttg cttgctccct
     8521 taccgttaga aaaacattgc ggacagtctg catcacgaat atgcaccgac cacacattcg
     8581 gaacaggatt taaccaagag gcttcttcaa tattaaaacc taaggttttt aatttatgct
     8641 gaaaggtcgc aatgctgtct tcaagtgcag catctttacc ggtaataaaa gtttgttctg
     8701 acatgataat acctttaact tgtcgtaaaa atggcaacat tctagcaaaa atccccatag
     8761 ataagtaaat tttttgtgaa atttctgctg aaagaacaga tttcaaaact tagaaaaaat
     8821 gattacaagc ggtcaaattt tacttgaatt ttgcaa
//
LOCUS       DANLUQ010000107         8721 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.108, whole genome shotgun sequence.
ACCESSION   DANLUQ010000107 DANLUQ010000000
VERSION     DANLUQ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8721
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.108"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            531..2918
                     /locus_tag="PXB27_001840"
     CDS             531..2918
                     /locus_tag="PXB27_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HDL5346601.1"
                     /translation="MEKMMRKINQLIISPYFFLSILSASANSDFWKSDLNFNLTNITK
                     RVGVDNFTTPVAGEPWAGIGPNGEAAGTAPLYYSRIPAMQVTEDNKLVVMFDLRWNRA
                     YDQDRIDPGIAISSDGGHTWTKKTAWSFDRTNHPARRSMDPTLLHNPIDNSLYVMHGT
                     WSMSDQQWYGGRVPHFNSGSWAATIYKSTDGGLNWEKNTEFSKFSNLDVFSKVTKNGK
                     PTLGFLGGVGSGIVMQDGTLVFPIQTAHQNGIATTIMYSKDNGKTWEMPEIDNPVAPN
                     QSSLENMVFELEPGKLVMTGRGNSRWAYSSTDMGKTWELFTPTNGLLPTSSQPTQGSS
                     IYVTLPNGRKVLLISKPDGKNDGWKRGDITLWMLDAKDPSHKHKVHIIRPGSGNAAGA
                     GYSSLAYKEGNLFVAFENDGDISVKNLTEHIAEMEAKAIEWGLPDELTPALDKINALP
                     YLNKAQKETLVEKMRRANDTAIAQSFVINKEMYNLKNNSDELDKLAKNITKALPSQQN
                     IYQRALAYVNKVTNTADTTYLNYNGIMDTYANLYESYLALNTKLDFTRYIQKARKFNE
                     YNTDILYRSFDNFFAHYDTGVKHNNLSLGLNTKLSENLRGGLFFEYSNKNRNSVQIGA
                     RAKYEMDNHQLSGFLRYRGVKHKDMLARNNSVDGYLNYAYRIQLDDKLSISPSVGAYV
                     SRSSRSLIDEDLAINSRTVYASDVGLNIHYKLNDIDAYIRPSIGFVNGDITLSQSNDM
                     TNTYKIKDKSNVYSVTTGLKKRFANGVALGADFKLHRYGSQTTESNFGLNVSYNW"
     gene            complement(3085..3621)
                     /gene="hpt"
                     /locus_tag="PXB27_001841"
     CDS             complement(3085..3621)
                     /gene="hpt"
                     /locus_tag="PXB27_001841"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDL5346602.1"
                     /translation="MEKHHIETLHSKEEVLNRINELAKEINQHYHQKQCQNLVVVGLL
                     RGSFMFMADLVRKLELPVEVDFLTASSYGSSTESSRDVKILKDLDGEIQGKDVLIVED
                     IIDTGFTLSKVGEILKLREPNSLAICTLLDKPCRREVDVPVKWVGFEIPDEFVVGYGI
                     DYAQKYRHLDYIAKVVIE"
     gene            complement(3660..5009)
                     /gene="pmbA"
                     /locus_tag="PXB27_001842"
     CDS             complement(3660..5009)
                     /gene="pmbA"
                     /locus_tag="PXB27_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HDL5346603.1"
                     /translation="MSITDKQTLLQQEQELRQAVEFALNFAKQAGAEAEVGVTKVAGL
                     SVSSRLEQVENIEFNNDGSLGISVYLGKRKGNASTSDLQPESIQKTVEAALAIAKYTS
                     EDACTGLADKELLAFEAEDLELYHQADISVDQAVELAIEAEHYALNADEKIVNSDGAT
                     FNSHSGVRVYGNTHGMLQSYLSSRYSLSCSVISAYEDQLERDYEYTIFREFDKLQAPK
                     WVGEQAAIKAVDRLNPQRIKTGEMSVIFYNDVATGLVGHLAGAISGGALYRKSSFLLD
                     KLGEQILPEWFAISERPHLLRQLASSAFDSEGVITQNREIITDGVLQTYLMTSYSGRK
                     MGLKSTGHAGGIHNWLVKPNRQGGLNTLLKEMGTGLLVTEMLGSAINSVTGEYSRGAA
                     GFWVENGEIQYPVAEITIAGQLQDMYKNLVAVGDDIEHRSNIQTGSILLEKMMVSGE"
     gene            5108..5638
                     /locus_tag="PXB27_001843"
     CDS             5108..5638
                     /locus_tag="PXB27_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HDL5346604.1"
                     /translation="MAKKRSKNEIDWTDEEEEIIWVSKSEIKRDSEHLKKIGAELIEL
                     TPQNLEKIPLDDDLKEAIRQAQGFKMEARRRQIQFIGKLLRNRDQEPIQEALDKVKNR
                     HNQQQALLHKLELVRNQLIAMGDASLDNLLTEYPQLDRQHLRNLIRGAIKEREANKPA
                     KNYREIYQYLKTEIVE"
     gene            5644..6015
                     /locus_tag="PXB27_001844"
     CDS             5644..6015
                     /locus_tag="PXB27_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HDL5346605.1"
                     /translation="MEYLSTILKAHIGLAYISLILLLTRGFLSSKMVDWRQYKILRIA
                     PHAVDTFLLITGIAAVAIFLAYDFFTLAQFSWLLPKLLFLVLYIVFATKAFKKGQPFS
                     LKFYLLAVVSFLLMMLTATFH"
     gene            6028..6741
                     /locus_tag="PXB27_001845"
     CDS             6028..6741
                     /locus_tag="PXB27_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDL5346606.1"
                     /translation="MIGPFVNAGAIILGGVLGACLGSHLPERIRTTLPLVFGLCCFGL
                     GMMLTASVKYMSAVALALILGTIIGELIYLENRIGKVAGLARGLIDRLFPPVHSLSQE
                     EFLEKFIALLVLFCVSGTGIFGAIKEGMTGDPSILYIKSILDFFTAMIFAASLGYAIA
                     AIAVPLIIVQGILILLATVIMPLTTANMMADFSATGGLLLFATGFRICGIKNFPVANM
                     LPALLLAMPMSYFWEWLVH"
     gene            6744..7307
                     /locus_tag="PXB27_001846"
     CDS             6744..7307
                     /locus_tag="PXB27_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HDL5346607.1"
                     /translation="MKKRIIVGISGASGFQYGYKVLELLKPFSEIETHLVISKGAELT
                     RTLETDYTREALHDLADQIHSLNNVGASIASGSFKTEGMVIAPCSMRTLAAIANGLSD
                     NLLTRAADVVLKERRKLVLMVREAPLNLAHIDNMRKVTEMGGIIFPPIPAFYQKPTSL
                     DEMVTHSVSHALSLLDIDIPNTPHWGE"
     gene            7370..7810
                     /locus_tag="PXB27_001847"
     CDS             7370..7810
                     /locus_tag="PXB27_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HDL5346608.1"
                     /translation="MHNPIPTHILDFIQTNHVVNIAAHTEQDFWAANCFYAFDQENVR
                     LIILTAKKTRHAGLMLENPHIVGTICGQIEKINEIEGVQFSATAECLEDKQAEKAALQ
                     IYYQKHPLARLKPSDVWVLSFNTIKHTGNKLMFAKKTIWERNII"
     gene            complement(7814..8689)
                     /locus_tag="PXB27_001848"
     CDS             complement(7814..8689)
                     /locus_tag="PXB27_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL5346609.1"
                     /translation="MMKTILIDEANQIKGFSHLYRLRSDLMLKYTEISVTETVEQEPA
                     EFEAGLRLVILLSGRSEIDFNSEVFELDASLKPQAAFLPLNRAEMGRKIFRKGQLQKE
                     LVIFLYPKWLIESQLNLASYHHFKQHLKAHQIILTQSMLKLIHDIVSEQEMQPFGLLE
                     KEGNLLALLASAFSQLTQAPRLKKDRVQQLTELLESGKFDGWSLKQIAIYFHTNVTTL
                     QNEFQHYHKTSIFAYLRRSKLEQAYKLLLQGQSITQAAEFAGYANSENFSTAFKRTFG
                     ITPSQIKRDKAKGII"
BASE COUNT         2630 a         1734 c         1621 g         2736 t
ORIGIN      
        1 ccttatctat caatacttga agccattaag ttcaagtctc gaccaggcac catttgacta
       61 aatttagttc aacagagcta aacgaaaaaa ccttagaaag cctgtaaaat caacgtttac
      121 aggctttttt gttgttttag ctcaatggca ctaaacattt catatcgcaa attttaagta
      181 gcctagcaaa ttttccgcca aatatgaccg cttgtaatgc cgttaggtta ttgcctggcg
      241 gcctttttgt tacagctctt atatttttta caccaaagtt cagaaaaagt cacttttatc
      301 actaacatta agatgaaacc ttgatacaaa gcttgggctt cggtgatatt taaaaaatat
      361 cacttttttc aataattgtg acaaatctct cagatttttt tatttttttg tgcttaaatc
      421 ttaagcataa aatgccgtta taattacttg ctatctggta ttttatacat aagattgaac
      481 catataatag ctgttcagct aaaaattagt ctttgcactt tatcccccta atggagaaaa
      541 tgatgagaaa aatcaatcag ttaatcatat ccccatactt tttcctaagt attttatctg
      601 cttccgctaa ctctgatttt tggaaaagcg accttaattt taatctcaca aatattacca
      661 agcgtgtcgg agtcgataac tttaccactc cagtagctgg cgagccttgg gctggtattg
      721 gtccaaatgg tgaagctgca ggtactgcgc cattatatta tagccgtatt ccagctatgc
      781 aagttaccga agataacaaa ttagttgtta tgtttgattt acgctggaat cgtgcgtatg
      841 accaagatcg tattgatcct ggtattgcga tttcttccga tggtggccat acttggacga
      901 agaaaaccgc ttggtctttt gatagaacta atcacccagc acgccgttca atggatccaa
      961 cgctattaca taatcctatt gataatagtt tatatgtaat gcacggaact tggtctatgt
     1021 cggatcaaca atggtatggt gggcgtgttc ctcactttaa tagtggcagc tgggcagcta
     1081 ccatttataa atctactgat ggtggtttaa attgggagaa aaatactgag tttagtaaat
     1141 tcagcaattt agacgtattt tctaaagtca ccaaaaatgg aaaacctacc ttaggctttt
     1201 taggcggggt cggatctgga attgtaatgc aagatggtac gttggttttc ccaattcaaa
     1261 ccgcacatca aaatggtatt gcgaccacta ttatgtattc taaggataat gggaaaactt
     1321 gggaaatgcc agaaattgac aacccagttg caccgaacca aagttcttta gaaaatatgg
     1381 tgtttgaact cgaaccgggt aagctagtga tgactggacg aggaaacagt cgctgggcat
     1441 actcttctac tgatatgggt aagacctggg aattatttac tccaactaat ggattattac
     1501 caactagctc acaaccaact caaggctctt ctatctatgt aacactacca aatggtcgaa
     1561 aagtgctact aatctctaaa ccagatggaa agaatgatgg gtggaaaagg ggtgatatta
     1621 cactttggat gctagatgca aaagatccat cgcacaaaca caaggtgcat attattcgtc
     1681 caggttcagg taatgccgct ggtgctggtt attcctcttt agcctataaa gaagggaatt
     1741 tatttgttgc ttttgaaaac gatggtgata tttcagtaaa aaatttaact gaacatattg
     1801 ctgaaatgga agcaaaagct atcgaatggg gattaccaga tgaacttact cctgctttgg
     1861 ataaaatcaa tgcactgcct tatctcaata aagctcaaaa agagacgcta gtcgaaaaaa
     1921 tgcgtcgtgc taatgatact gcgattgcac aatcttttgt gattaacaaa gaaatgtata
     1981 atctgaaaaa caatagtgat gagctggata aattggcaaa aaatattact aaagcactgc
     2041 cgtctcaaca gaatatttat caaagagcct tagcctatgt gaataaagtc accaatacag
     2101 cggataccac ttatttaaat tataatggta ttatggatac ctatgctaat ttatatgaaa
     2161 gctatttggc tttaaatact aaattagact tcactcgcta catccaaaaa gcgagaaagt
     2221 tcaatgaata taacactgat attttatatc gtagctttga taatttcttt gcccattacg
     2281 acacaggtgt aaaacacaat aatctatcct taggcttgaa tacaaaatta agtgagaatt
     2341 tacgtggtgg cttattcttt gaatacagta ataaaaatcg taatagcgta caaattggtg
     2401 caagagctaa atatgaaatg gataatcacc aactttctgg cttcttacgc tatcgtggcg
     2461 taaaacataa agatatgcta gcacgtaata acagtgttga tggctaccta aattatgctt
     2521 atcgtattca attagatgat aaattaagca ttagcccatc agttggtgct tacgtttcac
     2581 gttcttctcg ctcattaata gatgaagatc ttgcgattaa tagccgcacg gtctatgcaa
     2641 gtgatgtagg attgaatatc cattataagc taaatgatat tgatgcttat attcgtccaa
     2701 gtataggctt tgtcaatggt gatataactt tatctcaatc taatgatatg acaaacacat
     2761 acaaaataaa ggataaaagt aacgtgtatt ctgtcacgac agggttgaaa aaacgctttg
     2821 caaatggtgt ggcattaggg gctgatttca aattacatcg ttatggttct caaactactg
     2881 aaagtaattt cggtctaaat gtaagttata actggtaata ccgtaaactg attacgattt
     2941 aaaagttaga ctagcccaag actgagttct atattccata gagctcagtc ttttaatttt
     3001 tctttctcga taagaatatt ttatatttca accttaatcg agaatgtaat tagtcgtgaa
     3061 aatctaagta gtgattgagt ttttttattc aatcactact ttagcaatat aatctaaatg
     3121 acgatatttt tgggcataat caatcccata acccaccaca aattcatcgg ggatctcaaa
     3181 tcccacccat tttacgggca catcaacttc gcggcggcaa ggtttatcta ataaagtaca
     3241 aatagcaagt gaatttggct cacgtaactt gagaatctcc cctaccttgc ttaaggtgaa
     3301 gccagtatca ataatatctt ctacaatcaa tacatcttta ccttgaattt cgccatctaa
     3361 atcttttaga attttgacat cacggctgga ttccgtgctg ctgccatagc ttgatgcggt
     3421 tagaaaatcc acttctacag gtaactctag ctttcttact aaatctgcca taaacataaa
     3481 cgagcctcgc aataaaccaa ccactacaag gttttggcac tgtttttggt gatagtgttg
     3541 attgatttct tttgctaatt cattgattcg gtttaatact tcttcttttg aatgtagcgt
     3601 ttcgatatga tgtttttcca ttgatttctc caaataaaat tgattacaag cggccgtttt
     3661 tactcgcctg aaaccatcat tttttctaat aagattgaac ctgtttggat atttgagcgg
     3721 tgttcaatat catcgcctac cgccactaaa tttttataca tatcttgcag ttgccctgca
     3781 atggtaattt ccgctacagg gtattgaatt tcaccgtttt ctacccaaaa tcctgctgca
     3841 cctcgagagt attcccctgt tacgctatta atcgcagaac ctagcatttc cgttactaat
     3901 aatcctgttc ccatttcctt aagtaacgta tttaaaccac cttgacgatt tggtttaact
     3961 aaccagttat gaataccacc tgcgtgtcct gtacttttta accccatttt acgccctgaa
     4021 tagctagtca ttaaatacgt ttgtagcacg ccatcagtaa taatttcacg gttttgggta
     4081 attacacctt cactatcaaa cgcagaagag gctaattgac gcaacaaatg cggacgctct
     4141 gaaatcgcaa accattcagg cagaatctgt tcgcctaatt tatctaataa gaaacttgat
     4201 ttgcggtata atgcaccgcc actaatagct ccggccaaat gccccactaa accggttgcc
     4261 acatcgttat agaaaatcac agacatttcg ccggtcttaa ttcgttgcgg attcaatcta
     4321 tctaccgctt taattgcagc ttgctcccct acccatttcg gtgcttgtaa tttatcaaac
     4381 tcacggaaaa tggtgtattc ataatcacgc tcaagctgat cttcataagc tgaaatcacg
     4441 ctacaagaga gtgaatatcg gctcgacagg taactctgca acattccgtg cgtattgcca
     4501 taaacacgca cgccactgtg agaattgaaa gttgccccgt cactattcac aattttttcg
     4561 tctgcattta aggcataatg ctccgcttca atcgccaatt cgactgcttg atcaactgaa
     4621 atatccgctt ggtggtacaa ttccaaatct tctgcctcaa aggcaagcaa ttctttatca
     4681 gcaagcccgg tacaagcatc ttctgaggta tattttgcaa tcgcaagagc tgcttcaacc
     4741 gttttttgaa tcgactctgg ctgaagatcg gaggtggaag cattaccttt gcgtttgcct
     4801 aaataaaccg aaataccgag agaaccatcg ttattgaact cgatgttttc aacttgctct
     4861 aaacggcttg aaaccgaaag cccggcaact ttagtcacgc ctacttccgc ctctgcccca
     4921 gcctgttttg caaaatttaa ggcaaattcg accgcttgtc gtagctcctg ttcttgttgt
     4981 aacaaggttt gtttatctgt aattgacatt ttgttccatt gattgattgc attgatgaat
     5041 atattctaac tcaaatttct gttagaatac ggcatttttt agagtttatg taatgaaagg
     5101 ataaagaatg gctaaaaaac ggagcaaaaa cgaaattgac tggacagatg aggaagaaga
     5161 aatcatctgg gtcagcaaaa gtgagataaa acgagattct gaacacttaa aaaaaattgg
     5221 ggctgaattg atcgaattga ccccacaaaa tttggaaaaa attccactag acgatgattt
     5281 aaaagaggca attcgccaag ctcaaggctt taaaatggag gctcgccgcc gtcaaattca
     5341 atttatcggc aaattattac gcaaccgtga ccaggagccg attcaagaag ccttagataa
     5401 agtgaaaaat cgccataatc agcaacaagc cctattacat aaattggaat tagtgcgtaa
     5461 tcaactgatt gcaatgggcg atgcatcatt agataattta ttgacggaat atcctcaatt
     5521 agaccgccaa catttgcgta atttaattcg tggggcaatt aaagagcgtg aagccaataa
     5581 accggcaaaa aattaccgtg aaatttatca atatttaaaa actgaaatcg tggagtgatt
     5641 attatggagt acctatcaac cattcttaaa gctcatatcg gcttagctta tattagttta
     5701 attttattat taacccgtgg atttttatcg tcaaagatgg tggattggcg acaatataaa
     5761 attttgcgca ttgccccaca cgcagttgat accttcttac ttatcaccgg tattgctgct
     5821 gtcgctattt tcttagctta tgattttttc accttagcac aattcagttg gttgctacca
     5881 aagttactct tcttagtact ttatattgta tttgcgacaa aagcgttcaa aaaaggacaa
     5941 ccgttttcgc tcaaatttta tctgttagcc gtagtctcat tcttattaat gatgctaact
     6001 gccacttttc actaaataag ggaaactatg atcgggcctt ttgtgaatgc cggtgcaatt
     6061 attttagggg gcgtgctggg tgcttgttta gggagtcacc tgcctgaacg tattcgcact
     6121 acgttgccgc tggtattcgg cttatgctgt ttcggcttag gtatgatgct gactgcctca
     6181 gtaaaatata tgtctgcggt tgccttagca ttgattttag gcacgatcat cggtgaactg
     6241 atttatttag aaaacagaat cggtaaagtt gccggtttag ctcgtggttt aattgaccgc
     6301 ctattcccac cggtgcatag tctttcccaa gaagagtttt tagagaaatt tattgctctg
     6361 cttgtactgt tttgtgtgag cggtaccggc atttttggtg caataaagga aggaatgaca
     6421 ggcgatccaa gtattcttta cattaaatcg attctagatt tcttcaccgc aatgattttc
     6481 gctgcttcgt taggctatgc cattgcggcg attgcagtgc cgttgattat tgtgcaaggg
     6541 attttaattc tactggcaac cgtgattatg cctttaacca ctgccaatat gatggcagat
     6601 ttctccgcaa ccggcggttt actgctattt gcaaccggct ttcgcatttg tggaattaaa
     6661 aactttccgg tcgcaaatat gctaccagca ctactccttg cgatgccgat gtcttatttc
     6721 tgggaatggt tggtacatta atgatgaaaa aacgtattat tgtcggcatt agtggggcaa
     6781 gtggttttca atatggctat aaagtgcttg aactgctgaa accgttttca gaaattgaaa
     6841 cccaccttgt aatcagcaaa ggagctgaac tcactcgtac tctcgaaacg gattataccc
     6901 gagaagctct tcatgattta gctgatcaaa tacattcgct aaacaatgtc ggagcaagca
     6961 ttgccagtgg ttcatttaaa accgaaggta tggtcatcgc cccgtgttct atgcgaaccc
     7021 ttgccgctat tgccaacggt ttgagcgata atttgctgac ccgtgcggca gatgtggtgt
     7081 tgaaagaacg gcgtaaattg gtattaatgg tgagagaagc tccgctcaac cttgctcata
     7141 ttgacaatat gcgaaaagtc acagaaatgg gcggcattat tttcccaccc attccggcgt
     7201 tttaccaaaa gccaaccagc cttgacgaga tggtcaccca cagcgtaagc catgcccttt
     7261 ccctgttgga tattgacatt ccaaataccc cacattgggg agaataattc ggcttatctt
     7321 ttgctttttg aaatgctaga atacctgata aaaactatca ggtattttta tgcacaatcc
     7381 aatcccgacc catattttag atttcattca aactaaccac gttgtcaata ttgcagctca
     7441 taccgaacag gatttttggg cagcaaattg cttttatgcc tttgaccaag aaaatgttcg
     7501 cttgattatc ttaaccgcca agaaaaccag acacgctggc ttgatgcttg aaaacccaca
     7561 tattgtcggc acaatttgtg ggcaaattga gaagatcaac gaaattgaag gtgtccaatt
     7621 ctccgctacc gccgaatgtt tagaagataa acaagccgaa aaagccgctt tgcaaattta
     7681 ttaccaaaaa cacccgcttg cacggcttaa gcctagcgat gtttgggtgt tatcattcaa
     7741 taccatcaag cacaccggta ataaacttat gtttgcgaaa aagacgattt gggagagaaa
     7801 tataatttga agctcaaata atgcctttag ccttatcccg tttgatttgg cttggtgtta
     7861 tcccaaaagt tcgcttaaat gccgttgaaa aattctccga attggcatat ccggcaaatt
     7921 cagctgcttg tgtaatactc tgcccttgta atagtaattt ataggcttgt tctaatttag
     7981 aacgccttaa atacgcaaaa atagaggttt tgtggtaatg ctgaaattca ttttgtaatg
     8041 tggtcacatt ggtatggaaa tatattgcaa tctgcttcaa actccagccg tcaaatttac
     8101 cgctctcaag caattctgtt aactgttgca cccgatcttt ttttaaccgg ggagcctgtg
     8161 tcagctgaga aaaagcactt gctaataaag ctaatagatt accttctttc tcaagtaacc
     8221 caaatggttg catttcctgc tctgacacaa tatcgtgaat aagttttaac atactctgag
     8281 tcaaaataat ttggtgggct tttaaatgct gtttaaagtg atgatagctt gccaaattta
     8341 actggctttc tattagccat ttagggtaga gaaagataac aagctctttt tgaagttgcc
     8401 ctttcctaaa aattttacgc cccatttcag ctctatttag cggtaaaaat gcggcttgag
     8461 gttttaaact tgcatctaat tcaaatactt ccgaattaaa atcaatttcc gatcgaccac
     8521 tcaacaatat aaccaatctt agtccggctt caaattccgc aggttcttgc tctacggttt
     8581 cagtaactga aatctctgta tatttcagca taagatctga acgtagtcgg tataaatgtg
     8641 aaaaaccttt tatttggtta gcttcatcaa tcaagattgt tttcatcatt aaaaactttt
     8701 tttcgcatag tcaaaatttt a
//
LOCUS       DANLUQ010000108         8674 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.109, whole genome shotgun sequence.
ACCESSION   DANLUQ010000108 DANLUQ010000000
VERSION     DANLUQ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8674
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.109"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            137..4033
                     /gene="purL"
                     /locus_tag="PXB27_001849"
                     /gene_synonym="purI"
     CDS             137..4033
                     /gene="purL"
                     /locus_tag="PXB27_001849"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HDL5346610.1"
                     /translation="MTIQTFRGSPALSAFRIQQLLQKFQQNQLPVKSVYAEFLHFVAL
                     NRPLVADEENKIKELLHYGPTLEEDEPTGFCLIVTPRIGTISSWSSKATDIAHNCGLA
                     AVERIERGTAYYLEFSETPNESQLTTLKGLLHDRMMETVLDNESQAEQLFAQQEPKPF
                     TSVDILGGGRKALEEANINLGLALADDEIDYLVENFTQLGRNPNDIELYMFAQANSEH
                     CRHKIFNADWTIDGVKQEKSLFKMIKNTFEKTPDYVLSAYKDNAAVMEGSTVGRFFPD
                     QDGQYRYHQEDAHILMKVETHNHPTAISPFPGAATGSGGEIRDEGATGRGAKPKAGLT
                     GFSVSNLVIPKFEQPWENPLSKPNRIASALDIMIDGPLGGAAFNNEFGRPALLGYFRT
                     YEEKVNSFAGEEIRGYHKPIMLAGGIGNIRAEHVQKGEIPVGAKLIVLGGPAMNIGLG
                     GGAASSMASGKSKEDLDFASVQRENPEMERRCQEVIDRCWQMGAENPILFIHDVGAGG
                     LSNAMPELVHDGECGGKFDLRNILCDEKGMSPLEIWCNESQERYVLAVSPEKLDIFTA
                     LCERERAPFAVIGEATKEKHLTLADPHFDNKPIDLPMNVLLGKTPKMQRDVLSKTVKN
                     PPLATADIDLKEAFHRVLRLPAVAEKTFLITIGDRSVTGMVAQDQMVGPWQIPVADCA
                     VTTASLDSYHGEAMSIGERSPVALLDFAASARLAVAESITNIAATNIGDIKRIKLSAN
                     WMSAAGHEGEDAGLYEAVKAVGEELCPELGITIPVGKDSMSMKTTWEENGEQKSVTAP
                     LSLVISAFARVEDVRKTVTPQLRTDKGDTRLLLIDLGEGNNRLGATALAQVYKQLGDK
                     PADVVNVARLKGFFNAMQTLVAQGKLLAYHDRSDGGLITTLAEMAFAGNCGISVEIDR
                     LVDDLAVLFNEELGAVIQVRTEELSEVQAVLNAENLANLTFDIGTAVESDSFEITRNG
                     TTVFSQKRSELRGIWAELTHQMQRLRDNPECADQEFAAKKDPNNKGLSAFLTYDVNED
                     IAAPYINKGVKPSVAILREQGVNSHYEMAAVFDRAGFNAIDVHMSDLISGRRNLNDFN
                     AMVACGGFSYGDVLGAGGGWAKSILFNPMLRDQFSKFFANPNTLALGVCNGCQMVSNL
                     AEIIPGTENWPRFVRNKSERFEARAAMVKINDTNSLWFNGMAGSHMPIAVSHGEGQVE
                     FKNPQQLANLQQQNLVIAQYVDSHLNPTEQYPANPNGSALGITAIGNTDGRVAIMMPH
                     PERVFRAVSNSWCPEDWTEGGAWMRLFRNARVALKY"
     gene            4388..4570
                     /locus_tag="PXB27_001850"
                     /pseudo
     CDS             4388..4570
                     /locus_tag="PXB27_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248253.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            5019..5396
                     /locus_tag="PXB27_001851"
     CDS             5019..5396
                     /locus_tag="PXB27_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDL5346611.1"
                     /translation="MDKSQGVVSVNIRGGSGFGRANNAVGPNYMGMLAGKYQFNDSTW
                     IGALFGHSWRKISQNYKIGGGRKVTDSSIDLSKIDDLDKESSTTSPFNAAHLKQKPMS
                     RMAKLEYGDKYQTITLLPKSGSG"
     gene            6126..6902
                     /locus_tag="PXB27_001852"
     CDS             6126..6902
                     /locus_tag="PXB27_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HDL5346612.1"
                     /translation="MNYSATLSAYIHDLFTPFISYSQTHRAPNIKEIFFSNLGDYGVN
                     TALQPEQAKTTQFGINGFKERVFSEEDKLGFKVLFYNTHLKNYIYNVQHTAVGSAPSL
                     FIFHKNYEKPVNIKGIELEFSYDIGSFYGNLAYARQNTNQPVSFTDASSRVDAASHYG
                     FKTQGFGASKISALPRDYGSLELGTRWFDQRLQIGGVAKYYGKSKRIAIDEFNYTYYP
                     GTHIVKETHRLEETVNKQPIIVDFYVSYEPIKSLILKLEL"
     gene            7506..8000
                     /locus_tag="PXB27_001853"
     CDS             7506..8000
                     /locus_tag="PXB27_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL5346613.1"
                     /translation="MSRKYKFHEKSGAYFVSFATVYWVGVFVREIYFQVIVDALDYCR
                     KHKGMILYAYCIMPTHIHLLFQAENQNPSDLIRDFKTFTAKILIQRIKDNLQENRREW
                     LLWMFSRAAERRCNVKQYQFWQHHNQPIEIYSQKFFDEKLNYIHQNPVVSGFVSEPWE
                     WKYN"
     gene            8255..8494
                     /locus_tag="PXB27_001854"
                     /pseudo
     CDS             8255..8494
                     /locus_tag="PXB27_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651213.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
BASE COUNT         2662 a         1688 c         1884 g         2440 t
ORIGIN      
        1 cattagtatt gaacaaattt atttgatact aatgaataaa tgtatctaac agcaaacgtc
       61 tgacaaatat agcaatcgtt tgcgcaatcg tttataatcg gtttagtttt tcatcttaaa
      121 taggaaaatc caaagaatga caatccaaac cttccgtggc tcgcctgcct tatctgcttt
      181 tcgcattcaa caacttctgc aaaaattcca acaaaatcaa ttacctgtaa aatcagttta
      241 tgcggagttt ttgcattttg tggcattaaa tcgaccgctt gtagccgatg aagaaaacaa
      301 aatcaaagag ttattgcact acggtccaac gctggaagaa gacgagccga cagggttttg
      361 cctgattgtt acccctcgta tcggcacgat ttcctcgtgg tcgtctaagg caaccgacat
      421 cgcccataac tgcggtttgg cagcagtaga gcgaattgag cgtggcacgg catattattt
      481 agaattttct gaaacgccga acgaaagtca acttacgacg ttgaaaggct tattacacga
      541 ccgtatgatg gaaacggtgc tggataacga aagccaagcc gaacagcttt tcgctcagca
      601 agagccgaag ccgtttactt cggtggatat tttaggcggt ggtcgcaagg cactggaaga
      661 agcgaatatc aacttaggtt tagcgttggc ggacgatgaa attgattact tggtggaaaa
      721 ctttacccaa cttgggcgta accccaatga tattgaactc tatatgttcg cccaagccaa
      781 ctcggagcat tgccgccaca aaatttttaa tgcggattgg acgattgatg gggtaaaaca
      841 ggaaaaatcc ctgtttaaga tgattaaaaa cacctttgag aaaaccccgg actatgtgct
      901 ttccgcctac aaagacaatg cggcggtgat ggagggctca acggttgggc gtttcttccc
      961 cgaccaagat ggacaatatc gctatcatca agaagatgct catattttaa tgaaagtaga
     1021 aactcacaac cacccaaccg ccatctcgcc attcccggga gcggcgaccg gttcgggcgg
     1081 tgaaattcgt gatgaagggg cgacagggcg tggggcgaaa ccgaaagcgg gcttgacggg
     1141 tttttcggtc tctaacttag tgatcccaaa atttgaacag ccgtgggaaa acccgctttc
     1201 caagccgaat cgcattgcct ccgcactgga tattatgatt gatggcccac taggcggagc
     1261 ggcatttaac aatgaatttg gtcgtcctgc gttattgggc tacttccgta cttacgaaga
     1321 aaaagtgaac agcttcgcag gcgaagaaat tcgtggctat cacaagccga ttatgttagc
     1381 aggcggtatc ggcaacattc gtgccgaaca cgttcaaaaa ggcgaaatcc cggtgggagc
     1441 gaagttgatc gtgcttggtg gcccggcaat gaacatcggc ttaggcggtg gggcggcatc
     1501 gtcaatggca tcggggaaat cgaaagaaga tttggatttt gcctcggttc agcgggaaaa
     1561 ccctgaaatg gaacgccgtt gccaagaggt gatcgaccgt tgctggcaga tgggggcgga
     1621 gaacccaatt ttatttattc acgacgtggg ggctggcggt ttatcaaatg cgatgcctga
     1681 attggttcac gacggcgaat gtggcggtaa gtttgatttg cgtaacatct tgtgcgatga
     1741 aaaaggaatg tcgccacttg aaatttggtg taacgaatcg caagagcgtt atgtgcttgc
     1801 cgtttcccct gaaaaactcg acattttcac agcattgtgc gaaagggaaa gagccccatt
     1861 tgcggtgatt ggcgaggcaa cgaaagagaa gcatttaacc cttgctgacc ctcattttga
     1921 caataaaccg attgatttgc cgatgaatgt gttgctcggc aaaacgccaa aaatgcagcg
     1981 agatgttttg tcaaaaactg tgaaaaatcc accgcttgca accgctgaca tcgacttgaa
     2041 agaggcgttc caccgtgtgc tacgcttgcc tgcggtggct gaaaaaacct tcctgattac
     2101 catcggcgat cgctcagtaa cgggaatggt ggcacaagat cagatggtcg gcccgtggca
     2161 aattcctgtg gcagattgtg ccgtaaccac cgcttcatta gacagctatc acggtgaagc
     2221 aatgtcaatt ggcgagcgat cacctgttgc tttgcttgac tttgctgcct cagctcgcct
     2281 cgccgtggca gaaagcatca ccaatattgc cgccactaac attggcgaca tcaaacgcat
     2341 taaactctcg gcgaattgga tgtcggcggc agggcacgag ggcgaagacg cagggcttta
     2401 cgaggcagta aaagcggtgg gcgaagagct ttgccctgaa ttaggcataa ccattccggt
     2461 aggcaaagac tcaatgtcga tgaaaaccac ttgggaggaa aacggcgaac agaaatctgt
     2521 tactgctccg ctttcgctcg tgatttccgc tttcgctcgt gtggaagatg tgcgtaaaac
     2581 cgttacccca caacttcgca ccgacaaagg cgatacccgc ttgttgctga tcgacctggg
     2641 cgaaggcaac aaccgcttgg gtgcaaccgc acttgctcaa gtttacaaac aacttggcga
     2701 caaacctgcc gatgtggtga atgtggctcg cttaaaaggc ttcttcaacg caatgcaaac
     2761 ccttgtcgca caaggaaaat tgcttgccta ccacgaccgc tcggacggtg gtttaatcac
     2821 cacactggcg gaaatggcgt ttgcagggaa ttgcggaatt tcggtggaaa tcgaccgctt
     2881 ggtggacgat ttagccgtgt tattcaacga agaactgggg gcagtaatcc aagttcgcac
     2941 cgaagaattg agcgaggtgc aagcggtgtt aaatgccgaa aatcttgcaa atctcacctt
     3001 tgatattggt acagcggttg aaagcgacag ctttgaaatt acccgcaacg gcacaacagt
     3061 atttagccaa aaacgttccg aactgcgtgg catttgggcg gaactcactc atcaaatgca
     3121 gcgtttgcga gacaaccctg aatgtgcaga ccaagaattt gcagcgaaaa aagacccgaa
     3181 taacaaaggc ttatccgcat ttttaactta cgatgtgaac gaagacatcg ccgccccata
     3241 catcaacaaa ggcgtaaaac cgagtgtggc gattttgcgt gagcaaggtg taaacagcca
     3301 ctacgaaatg gcagccgtct ttgaccgtgc cggtttcaac gccattgacg tgcatatgtc
     3361 agacttgatt tcaggcagac gcaacctcaa cgacttcaac gcaatggtgg cgtgcggagg
     3421 cttctcttac ggcgatgtct taggtgcagg cggcggctgg gcgaaatcga ttctgttcaa
     3481 cccaatgctg cgtgaccaat tcagcaaatt cttcgccaac ccgaacacgc tggcattagg
     3541 ggtatgtaat ggttgccaaa tggtctccaa tctagcggaa attattccag gcacagaaaa
     3601 ctggccacgc ttcgtgcgta ataaatccga acgttttgaa gcccgtgctg caatggtgaa
     3661 aatcaacgac accaactcac tttggttcaa cggaatggca ggcagccata tgccgattgc
     3721 ggtttcacac ggtgaaggac aagttgagtt caaaaatcca cagcaattag cgaacttgca
     3781 acagcaaaat ctcgtgatcg cacaatatgt ggatagccac ctcaacccaa ccgaacaata
     3841 cccagccaac ccgaacggat cggcgttggg tatcaccgct atcggtaata cagacggacg
     3901 agtcgctatt atgatgccac accctgaacg tgtgttccgt gcggtcagta actcttggtg
     3961 cccagaagat tggacggaag gtggagcgtg gatgcggttg tttagaaatg cgagagtggc
     4021 gttgaagtat taaaaatcta ccttcgtagt gatctgcccc catagggggc gattaaaatt
     4081 cgaattcata gaacccgaat ttttatatat ctgataaacg aagttgttta tccttcgtgc
     4141 ttttcctgat atttcaagta ctttcttatc atttctgcat tgacatctac ggtgtctacg
     4201 cagtagcctc ttgccacaaa agtgggttgc cccacaattt atgcttacgt aaatagggaa
     4261 acttattgaa aaatcttatt ggaccctgtt caccctttta aatgccccat tacgtcttat
     4321 acagaaaatg aacgtgattt atttgaacgg tcaattctac cttaatagaa agtggttttt
     4381 agttagcaat caaaatcaca ttaacggaat tgagaatttg gagagtcagg caaaatgcta
     4441 tttgcgtaaa tttaacggcg ttcctaaagt acatgaattc tatttgaaag aatgcagatg
     4501 gcgttttaat cacagtaact taaaatatca agttttcttt ttaaaacaat tggttaaatt
     4561 gagtttctag gatagcccct tatttttata atcctctatt taagtgcata tttctattta
     4621 tgctaatcgt ctaacgtatc cttttattat aatattgaac atgattaata ttttcgtctt
     4681 gtgctatact agactcgtgc aataaaggca cacttctttc ttcagtgtct ttagcgttgc
     4741 gatttaaatt taattacatt ttttatagga aagacaatag aaatgaagat caaaaaatat
     4801 gtatttcacc tgacttatac aacacttatc tgccccttac ctagggtatt cgcagaggaa
     4861 ggttatatgt tagatacggt tgaggttaaa gcccaagcaa taaaaaaaca gatgaagttt
     4921 tcattaaaac aggggcaata agcacccgag ataaaatctt ggaatcaaac caaagcttag
     4981 agactattgt tcgttctatg cctggcgcat ttacacaaat ggataaatct caaggtgtgg
     5041 tttctgttaa tattcgagga ggttctggat ttgggcgagc taataatgca gtaggcccaa
     5101 attatatggg aatgctcgct ggcaaatacc aattcaatga ctctacttgg ataggagcat
     5161 tatttgggca tagttggcgc aaaatctctc aaaattataa aataggaggc ggacgaaaag
     5221 ttacggatag ctcgattgac cttagtaaaa ttgatgatct agataaagaa agttctacca
     5281 cctcaccgtt caatgcagct catttaaaac aaaaaccaat gagtcgtatg gctaaattgg
     5341 aatatggcga taaataccaa accatcacac tattaccaaa gtcaggtagc gggtagacat
     5401 ttaaaaaacc aaaacaacca gttaaattat cgttttcact tgcccgagtc accttggtta
     5461 gatgttcaag tggcctatgc ggaaaatcaa ggcaaacaaa actacaacaa agggagctga
     5521 attaataata aatatttaat tcaaggtttg gcaattaaca acaaagcgac aaccttaaat
     5581 ttgagtaaca cctttaaagt gtctccgttt aaagatggag aatgggaagc aatattaggt
     5641 tttaatcgtt taaagaaccg ctatagtaaa aatagacatc ctgcggaatt aaactataac
     5701 ttagagaatg gcgaaaccag cgaaaacaac gattctgtag gaatgacagc cgttcgtaaa
     5761 tcgctttata cttcaacttt ccagcctgat ggcgaacaga agttcgatac tttttatctc
     5821 gacaatcact ttaactatgg tatatgggga ttggatataa acgcaaatat taataccgct
     5881 cggtttattg gtcagcgttt taaatatttt ccttttgcga ttagagaatt ggagtcagat
     5941 attagacagg caagtagaaa taggcagttt gagcatttaa ggtcattaca atctcaatta
     6001 gacaacttgc aaaaaaacat tgtacatata ttccagatga ggattttcct aatgatcctg
     6061 aattgggaga gtttaactgt cctgatgtca attttcctca tacagcaaca ggaaaacata
     6121 aacgaattaa ttattcagca accttgtctg cttatattca cgatttattc acgcccttta
     6181 ttagttattc tcaaacacat cgagccccta atattaaaga aatattcttc tctaatttag
     6241 gtgactatgg tgtaaatacc gcactacaac cagagcaagc gaaaacaaca cagtttggta
     6301 ttaatggatt caaagaacgg gtatttagtg aagaagataa attaggattt aaagtgctat
     6361 tttataacac tcacttaaaa aactatattt ataatgtaca acataccgct gttggtagtg
     6421 ctcctagttt attcattttc cacaaaaact atgaaaaacc agtaaatatc aaagggatag
     6481 aattagaatt tagttatgat ataggctctt tctatggcaa cttagcttat gctcgtcaaa
     6541 atacgaatca acccgtatcc tttacagatg caagttcaag ggtagatgcc gcttctcatt
     6601 atggttttaa aactcaaggt tttggtgcca gcaaaatttc agcgttacca agagactatg
     6661 gctcattgga gttagggaca agatggtttg atcaaagact acaaattggc ggagtggcaa
     6721 aatattacgg aaaaagtaaa cgtattgcga tagatgagtt taactatact tattatcctg
     6781 gaacacatat tgtgaaagaa actcatcggc ttgaagaaac cgtaaataaa caacctatta
     6841 ttgttgattt ttacgtgagc tacgaaccaa tcaagtcgct tatattaaag ttagaactat
     6901 aaaatgtctt tgacaaaaag tacattgatc ctcttgatgc taataatgat gcagcaagtc
     6961 agagggttta caattctgat atcagtgatg aacctattta tacgctcaat aattatgcaa
     7021 gagggagaac tatagtgttt agcacaagtt ataaattcta aatggggttg tcctagataa
     7081 gctagcttaa atttccttta actaattatt tggcatagtg actaaaattg agtctgcaca
     7141 gtaggttaaa accttataat ataaggattt aacccagatg gtgcaagcgt ccacgcttgt
     7201 gccagttaat tcctgttctg aaagtatttc agtcataaga taaaagccta aatcagccaa
     7261 gtaaaatttg gtttatttgg gcttttgttt ttggcacaag cgtggacgct tgcgtcatca
     7321 ttttatttat ccatccattg ctatcatttc agtatttctt tggcttatca caagcggtta
     7381 aattttatca acactttgca agttttaatg atttttgcga tctagatcga aaaagttata
     7441 ttggcttgtg gaatttaatt ttgaataggg tatttttctt attccttttt aagttgagaa
     7501 aggctatgag ccgaaaatat aaattccacg aaaagagcgg tgcttatttt gtcagttttg
     7561 caactgttta ttgggttggt gtgtttgtga gagagattta ttttcaggta attgttgatg
     7621 ccttagatta ctgcagaaaa cataagggaa tgattttgta tgcctattgt attatgccaa
     7681 ctcatatcca tttattattt caagcagaaa atcaaaatcc ttctgattta atacgagatt
     7741 tcaaaacatt caccgctaaa atacttattc agagaattaa agataatttg caagaaaacc
     7801 gtcgggaatg gttgctgtgg atgttttctc gggcggctga acgacgttgt aatgtaaaac
     7861 aatatcaatt ctggcaacat cataaccaac ctattgaaat ctattcgcag aaattctttg
     7921 atgaaaagtt aaattatatt catcaaaatc cagtggtaag tggttttgtg tctgagcctt
     7981 gggaatggaa atataattga aataaactgc ttgtattttt gcgatctctg tcgaaaaatt
     8041 gtgaattttt acttgtctgt cggagaattt tgcgtaaatt ctgcttatta tgttttagtt
     8101 aaaggtattc tatgagtaga taacccaaat gaaaatagcg atataaccta tttctaccca
     8161 gtaattaatt cccgctttta tgtatcagat caaataattt acattgattt atcggtatta
     8221 tctgtcttta gtttttttaa ttaaggagcc ttatatgaaa ttcaaaaaat tagcattagc
     8281 agcaatgtta ggtggcttat ttgtaacagc taatgcggca gaattaccta atatcactat
     8341 tttagcgaca ggcggaacga ttgctggtag tcagcagaat gcaaatagta gtgcttatca
     8401 agcagggcaa ttgaatatcg aaaccttaat tgaagcagtg ccagaaatga aaacattggc
     8461 gaatatcaaa ggtgagcaga ttgtgaaaat tggcccacta aagccttgtt agatgtaggc
     8521 tatcaaggta ttgtcgtagc aggtgttggt aacggcaata tgagtgcgga aaatcttgca
     8581 ttatttagag gaagccaaca atatttttaa gatttttaat aaacgttaaa gcatataaaa
     8641 aatggcataa gtgagtcact tatgccatta tttt
//
LOCUS       DANLUQ010000109         8627 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.110, whole genome shotgun sequence.
ACCESSION   DANLUQ010000109 DANLUQ010000000
VERSION     DANLUQ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8627
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.110"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            162..350
                     /locus_tag="PXB27_001855"
     CDS             162..350
                     /locus_tag="PXB27_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346614.1"
                     /translation="MSYDANDALNEIEEALSELERVAEDLINNNPNKESELRGQGVHQ
                     ATKHLRFRIRNIRRGEAI"
     gene            391..693
                     /locus_tag="PXB27_001856"
     CDS             391..693
                     /locus_tag="PXB27_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346615.1"
                     /translation="MKIKLYFPDESIATIKRMGLRQMSPETLRWTADHPSSSYGMGAL
                     LRGKSGEILDGKSFAAMVHAFGAWIETDSEDTSRRVHNALVTAATGTEESVKVAKE"
     gene            693..2003
                     /locus_tag="PXB27_001857"
     CDS             693..2003
                     /locus_tag="PXB27_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL5346616.1"
                     /translation="MSNILNWPDYKVLQVSELEHDYQVHAEVSEPPTQCPHCNHPEIV
                     GFGRRDEVIMDTPVHGRRTGIMLNRRRYRCQSCRKTFLEPVPHKDEKRQMTNRLIQYI
                     ERESLRRTFSSVAEDVGVDEKTVRNIFNDYCERLEKTLNFEMPQWLGIDEIHIIKPRC
                     VITNIQQQTIVDMLDNRNKTTVTRYLSKRTDRDLVRYVAMDMWRPYRQAVETMIPDAT
                     VIIDKFHVVRMANESLERARKAIRSALTPQQRRGLMRDRFVLLKRRHELTDAEYMRFS
                     GWTLNYPEIGQAYELKEAFFEIWDCQTRHQAQEAYYSWLRQITPEMKAHYDPLIKAMG
                     NWHDDIFAYFDHPITNAYTESLNNLIRVVNRVGRGYSFEALRAKILFTEGFQKIKKPR
                     YQRQRIPEGAMGRMPFYGVAEAGPSTNYGADISTLVREIEAGRL"
     gene            complement(2099..3301)
                     /gene="tet(H)"
                     /locus_tag="PXB27_001858"
     CDS             complement(2099..3301)
                     /gene="tet(H)"
                     /locus_tag="PXB27_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(H)"
                     /protein_id="HDL5346617.1"
                     /translation="MNKSIIIILLITVLDAIGIGLIMPVLPTLLNEFVSENSLATHYG
                     VLLALYATMQVIFAPILGRLSDKYGRKPILLFSLLGAALDYLLMAFSTTLWMLYIGRI
                     IAGITGATGAVCASAMSDVTPAKNRTRYFGFLGGAFGVGLIIGPMLGGLLGDISAHMP
                     FIFAAISHSILLILSLLFFRETQKREALVANRTPENQTASNTVTVFFKKSLYFWLATY
                     FIIQLIGQIPATIWVLFTQYRFDWNTTSIGMSLAVLGVLNIFFQAIVAGKLAQKWGEK
                     TTIMISMSIDMMGCLLLAWIGHVWVILPALICLAAGGMGQPALQGYLSKSVDDNAQGK
                     LQGTLVSLTNITGIIGPLLFAFIYSYSVAYWDGLLWLMGAILYAMLLITAYFHQRKTT
                     PKAVISTP"
     gene            3393..4016
                     /gene="tetR(H)"
                     /locus_tag="PXB27_001859"
     CDS             3393..4016
                     /gene="tetR(H)"
                     /locus_tag="PXB27_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(H)"
                     /protein_id="HDL5346618.1"
                     /translation="MAKLDKEQVIDNALILLNEVGIEGLTTRKLAQKIGVEQPTLYWH
                     VKNKRALLDALAETILQKHHHHVLPLPNETWQDFLRNNAKSFRQALLMYRDGGKIHAG
                     TRPSESQFETSEQQLQFLCDAGFSLSQAVYALSSIAHFTLGSVLETQEHQESQKEREK
                     VETDTVAYPPLLTQAVAIMDSDNGDAAFLFVLDVMISGLETVLKSAK"
     gene            complement(4019..4537)
                     /locus_tag="PXB27_001860"
     CDS             complement(4019..4537)
                     /locus_tag="PXB27_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346619.1"
                     /translation="MPFKTVTFAQATTNDGQSLLAVNDLFIGPKSHTSAQYILQWNGA
                     EEVQSSSGIIVSTGLGSTGWFQSILAGAMAITGEASHPLLQGFSWSDRKLQFSVREPF
                     PSRTTGVALTFGTIEPDSPLQLGSLMPENGVIFSDGIEDDYLQFNAGCIAHIGIADIQ
                     GQLISQKGRQRI"
     gene            complement(4586..4765)
                     /locus_tag="PXB27_001861"
     CDS             complement(4586..4765)
                     /locus_tag="PXB27_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346620.1"
                     /translation="MFLVNQLLAITIATSAVLTNAQLAILAVAFWIAILPCARLATAV
                     GVWVFDDVVHNNSPN"
     gene            4854..5009
                     /locus_tag="PXB27_001862"
     CDS             4854..5009
                     /locus_tag="PXB27_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDL5346621.1"
                     /translation="MGLNRAEMMYREGAYVIDPVFPATLGYAGAGVVVAVGDDAGEFQ
                     IGDKVSV"
     gene            complement(5099..6646)
                     /locus_tag="PXB27_001863"
     CDS             complement(5099..6646)
                     /locus_tag="PXB27_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HDL5346622.1"
                     /translation="MKRRDFLRYGIGISLASSSLYSLANMMNHAQHGNMAMMHSVPTG
                     LMPTEAMPSGLSLNGILPKLANQSTQAGLFKATLKAEPIKIRLADNKETEFWAYNGQL
                     PGPQIEVFEGDTVEIEFINHLPQPSTVHWHGLDVPNEADGNPMDMVEPQGKKVYRFTL
                     PQGSAGTYWYHPHPHDHVSEQVYKGLAGTFVVKAKNDPLAHLPEQHWVISDLRLNADG
                     TIPANTMLDWINGREGEFVLINGQYQPQIQVKTNERIRLWNATSARYFRLNIPGVKWI
                     VVGTEGGLLEKPRSPVDELLLTPAERVEVIMVGETQGTVNLQSGYYDRRKMMVQEQPK
                     DLTLATIQVKSEPVQLPESLRSLPNWDEPKQVRQIRFSEKMMNHTNMPMMNHGTHHAT
                     TTLTDNPIPPMMNGMFLINGQTFDMNRIDFVTKLNEVEQWEIFNESHMDHPFHLHGTQ
                     FEVIQHTLNGKTFKPEGRALKDVVNLRPYEKVIIRFKQGHTGLKMYHCHILEHENLGM
                     MGMFKVE"
     gene            complement(6660..7115)
                     /locus_tag="PXB27_001864"
     CDS             complement(6660..7115)
                     /locus_tag="PXB27_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005819327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HDL5346623.1"
                     /translation="MKLHKFTRSLLLLSLGITAFAQAQTLSMEVWKSPTCGCCNEWIS
                     YMQKNGFEVKVNETGNYDAHKRFNIKYEHASCHTAVIDGYVIEGHVPAEDIKRLLAEK
                     PDAIGLSAPGMPIGSPGMDGEAYGGRKDPYDVVLIKKNGESEVFKSYNQ"
     gene            complement(7297..7617)
                     /locus_tag="PXB27_001865"
     CDS             complement(7297..7617)
                     /locus_tag="PXB27_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346624.1"
                     /translation="MELKVIHKNLVDSAKIKLHAVYLVIFLFVSHFAMIFGMADPISA
                     GYQPTTPAMTHSMQMDAKQITEMHNQMMTGEITPEEMMKMHQEMNPNMNMPNTQGGHS
                     EHHK"
     gene            7616..8383
                     /locus_tag="PXB27_001866"
     CDS             7616..8383
                     /locus_tag="PXB27_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL5346625.1"
                     /translation="MSKIIKIGKVANNSDAIDAISTQPRHSVIVGTIKRLQTDRIDLL
                     YQHRVDPNVPIEEVAGAIKDLMKQGKVLNWGLSEAGEKTIRKAHAIQPLTAVQSEYSL
                     FFRGREKDVILVCEELGIGFVPWSPLAMGALAGYVDENSRFEADPSKDLRGIIPRFTP
                     EAMQQNVKLIQLVQKWAKRKLCTPAQLSLAWLMAQKAFIAPIPGTSKARHLLDNLGAD
                     DVKFTEEELAQFRQELEAIEIVGLRLPEAILNFSEAK"
BASE COUNT         2500 a         1846 c         1902 g         2379 t
ORIGIN      
        1 tttagcggtt gatctgcgct agacgtaatt ttgtaaataa accggatttt agggttgacg
       61 gaaaaggcca gatgccctaa tattagattc aagagctgag aacaacggct cgggcataag
      121 cccaacgggc gcaccgccga acttggtgca ggatagaagc catgtcatac gatgcaaatg
      181 atgcgctgaa tgaaatcgaa gaggcgctta gcgagcttga aagggttgct gaagacctaa
      241 tcaacaacaa cccgaataaa gaatcagaat tacgcggcca aggggttcac caagcgacaa
      301 agcacctccg ttttcgtatt cgcaacatcc gccgcggcga agcaatttga ctaacatgcc
      361 gccctagggg cggctttcag cgaggtcagc atgaaaatca aactctattt tccggacgag
      421 tctatcgcca cgataaagcg aatggggctt cgccaaatgt ctcccgagac tctcaggtgg
      481 actgccgatc atccgtcaag cagctatggt atgggcgctt tgctcagagg caagtctggt
      541 gaaatccttg atgggaagtc ttttgctgca atggtgcatg cctttggcgc gtggattgaa
      601 acagacagtg aagataccag tcgtcgagtt cataacgcgc tagtcactgc tgccactggc
      661 accgaggaat cggttaaggt ggcaaaggag taatgtcgaa tatcctcaac tggcctgatt
      721 acaaagtgct ccaggtatcc gaactggagc acgattacca ggtacacgcc gaagtttccg
      781 aaccgcctac gcagtgcccc cactgcaatc atcctgaaat cgtcggcttt ggtcgccgtg
      841 atgaggtgat catggataca cccgtccacg gaaggcgtac cggcatcatg cttaaccgcc
      901 gtcgctatcg ctgccagtct tgccgaaaga cgtttctgga gcctgtaccg cacaaagacg
      961 aaaagcggca gatgaccaac cgcctgatcc agtacatcga gcgcgagagc ctgcgccgca
     1021 cgttctccag tgttgccgag gatgtgggcg tcgatgaaaa gaccgtccgt aacattttca
     1081 acgactattg tgaacgcctc gaaaagacgc tcaattttga aatgccgcag tggttgggta
     1141 tcgacgaaat ccacatcatc aaaccccgct gcgtgatcac caacattcag cagcagacca
     1201 ttgtggacat gctggataac cgcaataaaa ccaccgtcac ccgctacctg agcaaacgca
     1261 ctgaccgcga cctggtgcgc tacgtggcaa tggatatgtg gaggccgtac cggcaagcag
     1321 tagaaaccat gattcctgac gccacggtga tcatcgacaa gttccacgtt gtcaggatgg
     1381 cgaacgaatc actggaacgc gccagaaaag ccatcagaag cgccctgacg ccacagcagc
     1441 gccgtgggtt gatgcgagat aggtttgtcc tactgaaacg ccgtcacgag ctgacagatg
     1501 ccgaatacat gcgtttttca ggctggacat tgaattaccc tgagattggc caagcctacg
     1561 aactgaaaga agcctttttc gagatttggg actgtcagac ccggcaccag gcacaggagg
     1621 catattactc ttggctgcga cagattacgc cggaaatgaa agcccactat gacccgctta
     1681 tcaaagcgat gggcaactgg cacgatgaca ttttcgctta cttcgaccac cccattacca
     1741 acgcctacac cgagtcactg aacaacctga ttcgtgttgt taatcgtgtt ggccgtggct
     1801 acagctttga agcgctgcga gccaagatcc tgtttactga gggcttccag aagatcaaga
     1861 agccacgcta tcagcgtcaa cggatacccg agggcgctat gggcaggatg ccgttctatg
     1921 gcgttgccga agcggggcca tccactaact acggcgctga catatcaaca ctggtgcggg
     1981 agattgaggc ggggcgttta tagccctgtt tcaaccctgt aatccggata cccaaataaa
     2041 gtcctatcaa cgttattaag agcggacagg acttttatga tctttataat ttctagtatt
     2101 aaggggttga aataacagct ttaggtgtgg tttttctttg gtgaaaataa gcggtaataa
     2161 gcaacatagc ataaagtatt gcccccatca gccataacag accatcccaa taagcgacgc
     2221 tataactata aataaaggca aataaaaggg gaccaatgat cccggtaata ttggttaggc
     2281 tcaccagagt accttgtaat ttcccttgcg cattatcatc gacagatttt gataaataac
     2341 cttgtaatgc gggttgcccc atacctcctg ccgctaagca aattaatgct ggtaagatga
     2401 cccaaacgtg gcctatccac gctaataata aacagcccat catatcaata gacatactga
     2461 tcataatggt ggttttttcg ccccattttt gtgccaattt cccagcgaca atcgcctgaa
     2521 agaaaatatt taatacaccc agaaccgcca aagacatacc gatagaagtt gtgttccaat
     2581 caaaacgata ttgtgtaaac agcacccaga tggtggcagg aatttgcccg ataagctgga
     2641 taataaaata ggttgctaac caaaagtaga ggcttttctt aaaaaaaaca gtgactgtat
     2701 ttgaggcagt ttggttttca ggtgtcctat tggcaacaag cgcttctctt ttttgtgttt
     2761 ctcggaaaaa gagcaaagag agtattaata atatcgagtg tgaaatagcg gcaaaaataa
     2821 atggcatatg agcactgata tcacctaata atccccctag cattgggccg ataataaggc
     2881 caacaccaaa agcaccacct aagaaaccaa aatagcgagt tcgattttta gcgggagtca
     2941 catcactcat cgctgatgca catacggcac ctgttgcgcc tgtgatcccc gcaatgatgc
     3001 gcccaatata gagcatccaa agtgtggttg agaatgccat taaaagatag tcgagtgccg
     3061 cgcctaaaag ggaaaacagc aagatgggtt ttctgccgta tttatcagac agtcgtccta
     3121 gaataggagc aaaaataacc tgcatggtag catagagcgc taatagcaca ccgtaatggg
     3181 ttgccagtga attttcactg acaaattcat ttaatagagt agggagtact ggcatgataa
     3241 gcccgatacc aatggcatct aatacggtga tcagcagtat aataataatt gatttattca
     3301 ttgagatcct aaaaatctat cagtgataga gtgggtgtaa tatatctcta tcagtgatag
     3361 attgtcaagg ctattttatt ttgaggtagg taatggcaaa gctagataaa gaacaagtta
     3421 ttgataatgc gttgatttta cttaatgaag ttggtattga aggattaaca acgcgtaagc
     3481 tggcgcaaaa aataggtgtg gaacaaccca cattgtattg gcatgtaaaa aataaacgcg
     3541 ctttgttaga tgcattagca gaaactattt tgcaaaagca ccatcatcat gttttgccat
     3601 tgccgaatga aacatggcag gactttttgc gaaataacgc gaaaagcttc cgccaagcct
     3661 tattaatgta tcgtgatggt ggcaaaattc atgcgggaac acgcccctct gaaagtcaat
     3721 ttgagacatc agaacagcaa ctacagtttt tgtgtgatgc tgggtttagt ctatctcaag
     3781 ccgtgtatgc attaagctct attgcgcatt ttacattagg ctccgtactg gaaactcaag
     3841 agcatcaaga aagccaaaaa gagcgtgaaa aagtagagac ggatactgtt gcctatccgc
     3901 cattattaac ccaagccgtt gcaattatgg atagtgataa tggtgatgct gcatttttgt
     3961 ttgtccttga tgtgatgatc tctggacttg aaacagtatt aaagagcgct aaataaatct
     4021 aaattcgctg acgccctttt tggctaatta gttgcccttg tatgtcagcg ataccaatgt
     4081 gagcaataca acctgcatta aattgtaaat agtcatcttc gatgccatca gagaaaatta
     4141 cgccattctc tggcatcaaa gatcctaatt gcagtggtga gtcaggctca atagtgccaa
     4201 aagtcagtgc aacacctgtt gttctacttg gaaatggctc tcttacacta aattgtagct
     4261 ttcgatcact ccagctaaag ccttgtaata gagggtgcga agcttctcct gtaattgcca
     4321 tcgcaccagc aagaatagat tgaaaccacc ccgttgatcc caatcctgtt gatacaataa
     4381 tgcctgatga agattgcact tcttcagcgc cattccattg caaaatatac tgtgcggaag
     4441 tgtggctttt agggccaata aataagtcat taaccgctaa taaggattga ccatcattgg
     4501 ttgttgcttg tgcaaaagtg accgttttaa atggcatttt tttattaatg ggtttaaaaa
     4561 cggtttttgt tttgataatc aacctttaat ttggagaatt gttatgaacg acatcatcaa
     4621 aaacccaaac gcccactgcg gttgccaatc ttgcacaggg caaaattgca atccaaaatg
     4681 caactgccaa aatggcgagc tgtgcatttg tcaaaactgc acttgtggcg attgtgattg
     4741 caagtaattg attaactaaa aacattgggc gggcttaggt ttgcccaatg tttgccaaat
     4801 cccattttga taaggaaaac tataatgaaa atgtttaaaa accgtgccgt gcattgggac
     4861 taaaccgtgc cgagatgatg tatcgggaag gggcgtatgt gattgaccct gtgttccctg
     4921 cgactttggg ttatgcaggg gcaggcgtgg tcgtggcagt gggcgatgac gctggcgaat
     4981 ttcaaatcgg cgacaaagtg agcgtctgat ctgcacccca aaagttggac taaaaaccaa
     5041 ctgattaagg tgcagatttt ttatgacgaa atccctctat ttttatagtt agataccact
     5101 attccacttt aaacatcccc atcataccga gattttcatg ttctaaaata tggcaatggt
     5161 acattttcaa cccagtatgt ccttgcttaa agcgaataat cactttttca taagggcgta
     5221 aattaaccac atcttttaac gctctgcctt ctggcttaaa ggttttaccg tttaacgtat
     5281 gttgaattac ttcaaattga gtgccgtgta aatggaatgg atgatccata tggctttcat
     5341 tgaagatttc ccactgttcc acttcattca atttggtaac aaaatcaata cggttcatat
     5401 caaaggtttg accgttgatt aagaacatac cattcatcat tggtggaata gggttgtccg
     5461 ttagtgttgt agtagcgtga tgtgtaccgt gattcatcat tggcatattc gtatggttca
     5521 tcattttttc actaaagcga atttggcgaa cctgctttgg ctcatcccag ttaggcaagg
     5581 accttaagct ttcaggtaac tgaacaggtt ctgatttcac ctgaattgtg gctaaagtga
     5641 gatctttagg ctgttcttga accatcatct tacggcgatc ataataaccg ctttgtaaat
     5701 tgactgttcc ttgcgtttca cccaccataa tcacttcaac gcgttcggct ggtgtaagta
     5761 gcagttcatc aacaggcgag cgaggttttt caagtaaacc accttcggta cccaccacaa
     5821 tccatttcac ccctggaatg tttaaacgaa aataacgagc gctagtggca ttccaaagac
     5881 ggattcgttc attcgttttc acttgaatct ggggctgata ttgaccgttg attaacacaa
     5941 attcaccctc acgaccgttt atccaatcta acatcgtgtt tgctggaatg gtgccatcgg
     6001 catttaaacg cagatcggaa atcacccaat gttgttcagg aaggtgtgca agcggatcat
     6061 ttttcgcttt aacaacgaaa gtgcctgcta agcctttata gacttgttcg gaaacatggt
     6121 catgtggatg agggtggtac caatatgtcc cagcactgcc ttgaggtagc gtaaagcgat
     6181 agactttttt cccttgcggt tcaaccatat ccatcgggtt accatccgcc tcatttggta
     6241 catctaagcc gtgccaatgt actgttgagg gttgtggtag gtgattgata aattcaattt
     6301 ctacggtatc tccttcaaat acttcaattt gtggccctgg tagttgtccg ttataagccc
     6361 aaaattccgt ttctttattg tctgcgaggc gaattttaat tggctctgct tttaaagtgg
     6421 ctttgaacag tccagcttga gtcgattgat tagcaagttt tggtaatatc ccattaagcg
     6481 ataaacctga aggcattgcc tcagttggca ttaggccagt aggtactgaa tgcatcatcg
     6541 ccatattgcc atgttgtgcg tggttcatca tattcgcgag gctataaagc gagctactgg
     6601 ctaagctaat tccaattcca taacgtaaaa aatctcgacg tttcattctt tccccccccc
     6661 tattggttat aggatttaaa cacttcactt tcaccgtttt tcttaatcaa aacaacatca
     6721 taaggatctt tgcgtccgcc gtatgcttca ccgtccatac ctggtgaacc aattggcatt
     6781 cccggtgcag acaagcctat tgcatcgggt ttttctgcga gtaaacgttt gatatcctca
     6841 gcaggtacat gtccttcaat cacatagcca tcaatcacgg ctgtgtggca agaagcatgt
     6901 tcatatttaa tgttaaagcg cttatgagca tcataattgc ctgtttcgtt gactttaacc
     6961 tcaaaaccgt ttttctgcat atagctgatc cattcattac aacagccaca agtcgggctt
     7021 ttccacactt ccatagaaag tgtttgtgct tgagcaaaag cggttatgcc taaacttaaa
     7081 agcaacaatg agcgggtgaa tttatgtaat ttcataagat gtccttattt tttattgaga
     7141 ttgaatggac atcattttaa agcttaacct agggttaagg tcaagtaatc tgaaaaaatt
     7201 ctatcatttg aaaatttttc tcaaaatcct actgcttgta gtagagatat gccaacgtac
     7261 catccactat tattttttaa aatgtgatca gaattattat ttatgatgtt ctgaatgacc
     7321 accttgagta ttcggcatat tcatattagg gttcatttct tgatgcatct tcatcatttc
     7381 ttcaggcgtt atttcgcccg tcatcatttg gttgtgcatt tctgttattt gtttagcatc
     7441 catttgcatt gaatgagtca ttgctggtgt agtgggttga tagcctgctg aaataggatc
     7501 tgccatgcca aagatcatag caaagtgact cacaaataag aaaatcacaa gataaaccgc
     7561 atgtaattta atttttgcac tatccacgag atttttatga ataactttta attccatgag
     7621 taaaataata aaaataggaa aggtagctaa taatagtgat gcaattgatg cgatatctac
     7681 ccaaccacgc cattcagtaa tagtcggtac gataaaacgc ttacaaaccg accgtattga
     7741 cttactctat caacaccgtg ttgatcccaa tgtgccgatt gaagaggtgg ctggcgctat
     7801 taaagattta atgaaacaag gcaaagtttt gaattggggc ttgtcggaag caggcgagaa
     7861 aaccattcgc aaggcacacg ctattcagcc tttaaccgcc gtacaaagcg aatactcgtt
     7921 gtttttccgt ggtcgtgaaa aagatgtcat tctcgtttgt gaagaattag ggattgggtt
     7981 tgtgccttgg tcgccgctgg caatgggagc attggctggt tatgtggacg agaacagccg
     8041 ttttgaggct gatccaagca aagatttgcg tggtattatc ccacgcttta cacccgaagc
     8101 gatgcaacaa aatgtgaagt taatccagct tgtgcaaaaa tgggcaaaac gcaaactttg
     8161 tacgcctgca cagctttctt tggcctggct aatggcacag aaagcgttta tcgcgccaat
     8221 tcctgggacg agcaaagccc gtcatttgtt ggataattta ggcgcagacg atgtgaaatt
     8281 taccgaagaa gaattggcgc aattccgcca agaattagaa gccattgaaa ttgtcggctt
     8341 gcgtttgcct gaagcaattt tgaatttttc agaagcgaaa tagttttcag atacctgaaa
     8401 aagtcgtaaa taaaaattct ttcttacagc ggtcgttttc ataaaatcct ttgaaaaacg
     8461 atcgcacttt ttcttgaata tgtagcaaga ttagtggaag atttatcgaa attgaaagat
     8521 attgggaaaa gggtaaaact ggaatcagac agatgattag gcaaaacgct atagtcaata
     8581 agtgtaaaaa tggcataatc aactcaatga atttatggac aaatatt
//
LOCUS       DANLUQ010000110         8552 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.111, whole genome shotgun sequence.
ACCESSION   DANLUQ010000110 DANLUQ010000000
VERSION     DANLUQ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8552
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.111"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            274..1428
                     /locus_tag="PXB27_001867"
     CDS             274..1428
                     /locus_tag="PXB27_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HDL5346626.1"
                     /translation="MNQFFPLIFSLTLFSGVTLALEKAPRTMPTEGINKPLLGQILFF
                     DNMLSHNGSQNCSSCHNPDYAFIDNRTTPAKGVVSQGDTPTKFGKRNSPTMLYAKYSP
                     EFHFDEQSQEYIGGQFWDGRAKNLAEQAGMPPIDPNEMGMPNKLEVAKRLYNVPMYQR
                     FLGQFYGEKVWESYESVYAAMEDAIATFQQENKLLAPFDSKYDLFLKGEVQLTALEEK
                     GRALFFNKAQTNCSSCHQLQSAANHQEETFSNYHYYNVGVPGNPDLIAVNGFAPDFVD
                     LGLFENPNVKGDETQKGKFKTPTLRNVAVTALYMHNGVFKELKTVLLFLDSYNNPERK
                     INPETGTAWQQAEFEPTIAHQKLKAKPLSDEEIDALEAFLKTLTDERYRK"
     gene            complement(1476..2465)
                     /locus_tag="PXB27_001868"
     CDS             complement(1476..2465)
                     /locus_tag="PXB27_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HDL5346627.1"
                     /translation="MNDLIIYNTEDGKSNVALLVVENEAWLTQNQLAELFDTSVQNIS
                     EHIKNILKDKELDENSVIKDYLITAQDGKNYQVKHYSLEMILAIGFRVRSPRAVQFRK
                     WANSTLRTYLEKGFLIDSERLKNPQGRLDYFDELLEQIREIRASELRFYQKVRELFKL
                     SSDYDKTDKNTQMFFAETQNKLIYAITKHTAAELICQRADATKMNMGLTSWKGKVVRK
                     GDIVIAKNYLDNNELDSLNRLVMIFLESAELRVKNNQDLTLGFWRRNVDMLIEFNGFS
                     VLGNGGTITHKQMESFVREQYEKFDIQRKCLKQKEADWEDLQALEKLESELTR"
     gene            complement(2625..3989)
                     /gene="mpl"
                     /locus_tag="PXB27_001869"
     CDS             complement(2625..3989)
                     /gene="mpl"
                     /locus_tag="PXB27_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HDL5346628.1"
                     /translation="MKKHIHILGICGTFMGGVAMIARELGYKVTGSDTNVYPPMSTFL
                     AAHGIEIIPNYDVAQLQPAPDLVVIGNAMSRGNPCVEYVLDNQLNYTSGPQWLHDHLL
                     KDKWVLAVSGTHGKTTTTGMLAWILDQNGIDTGFLIGGVAGNFGISARAGNSPFFVIE
                     ADEYDSAFFDKRSKFVHYTPKTLIINNMEFDHADIFDDLKAIQRQFHHLVRTMPQSGC
                     ILSAIADQNVQDTLKMGSYSQLQFIGEQNEWFAKPLNADCSQFEVFHLGVKQGEVHWS
                     IIGKHNMHNALMAIAAAHHAGVEVQGACEALGKFVNANRRLEVKGEVNGITVYDDFAH
                     HPTAIEATIDALRGKVGKNQRILAVLEPRSNTMKMGVHKDEIAPSLHDAEAVFVYQPD
                     IIAWSVSEITNVLSQPAYWSANIDDLVDLVVKEAKPTDHILVMSNGAFGGIHQKLLAK
                     LADK"
     gene            complement(4285..5040)
                     /locus_tag="PXB27_001870"
     CDS             complement(4285..5040)
                     /locus_tag="PXB27_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005613463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HDL5346629.1"
                     /translation="MKLKSFKPMFMLLSVAFLTACSSSINPETGVRDDPLEGFNRAMW
                     KVNYDYLDPYVLKPVAKGWKEYVPSPVKTGLINVANNLDEPVSFVNRLIEGEGKKAMV
                     HFNRFWINTVFGLGGLIDWASLDEGLRLNNGQRGLGESLGAHGVPSGSYIMMPAYGAT
                     TPRQGIGSAVETGYSVLTYVGSPWSIAKFVVQGVDTRSKLLEQDALLEQAQDPYVTFR
                     EAYFQNLEFKVKDGNVEQSQQEQLSEEELKNID"
     gene            5195..5512
                     /gene="metJ"
                     /locus_tag="PXB27_001871"
     CDS             5195..5512
                     /gene="metJ"
                     /locus_tag="PXB27_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HDL5346630.1"
                     /translation="MADWNGEYISPYAEHGKKSEQVKKVTVSIPIKVLEILTNERTRR
                     QIKNLRHATNSELLCEAFLHAFTGQPLPNDEDLMKDRADEIPEAAKQKMCELGIDPDE
                     WQY"
     gene            5560..5790
                     /locus_tag="PXB27_001872"
     CDS             5560..5790
                     /locus_tag="PXB27_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF997 family protein"
                     /protein_id="HDL5346631.1"
                     /translation="MYQQLTTEARWAVLLTLIYLLGWVGFAYFSPQGRGIFGFPIWFE
                     LSCIFLPVLFSLVATIVVKKVYKNIDLEVNNE"
     gene            5783..7207
                     /gene="panF"
                     /locus_tag="PXB27_001873"
     CDS             5783..7207
                     /gene="panF"
                     /locus_tag="PXB27_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HDL5346632.1"
                     /translation="MNSEMILPLIAYLIFVFGVAFYAYRKRQGGSFISEYYIGGRSMS
                     GFVLAMTTAATYVGASSFIGGPGAAYKYGLGWVLLAMIQVPAVLLSLGALGKKFALLA
                     RKHNSVTINDMLLARYQNKFVVWIAGLALLLSFFAMMTVQFIGAGRLLETTLGIPYQT
                     AVMIFAITVGLYTFIGGFRAVVLTDTIQGLVMIVGTSILLAGVIYATGGVENAMHTLE
                     AINPQLTTPYGIDERPLDFTFMTSFWVLVCFGLIGLPHIAVRSMACKNSQALHRAMII
                     GTVVIAILMFGMHLSGVLGRAVVPDLKVPDQVIPTLMVQVLPPVVAGIFLAAPMAAIM
                     SSIDSMLIQSSSTLIKDLYLAIKPNAINNEKKIKLFSTITTLTFTVLLVFAALTPPEM
                     LIWLNLLSLGGLEATFLWVIVLGLYWEKANATGAIYSMLAGLSCYVLMTTFKIAIFSF
                     HAIVPSLLIGLIAFLIGNRFAPKV"
     gene            complement(7284..8474)
                     /gene="nhaA"
                     /locus_tag="PXB27_001874"
     CDS             complement(7284..8474)
                     /gene="nhaA"
                     /locus_tag="PXB27_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HDL5346633.1"
                     /translation="MEKLKEFLKSEASGGVLLLVFAFAAIVCANTSLSDFYFEFLQTI
                     VSVQFGEFALNKPLLLWVNDGLMAVFFTLVGLEVKKELFEGSLSSYKKAIFPAIAAVG
                     GMIVPAIIYWAINQNHPEYHNGWAIPMATDIAFAVGIVALLGTRVPLALKAFLLALAI
                     IDDLGAIVVIAIFYSQEISVQALTIAAIAIIGLVLLNRFKVTSLCAYLVVGLVLWTSV
                     LKSGVHATLAGVIIGFCVPLKGKHGERPAEELEHILAPWCAYMILPIFAFANAGVYLL
                     EMGMEQLSSPLPLGIALGLIIGKTLGVFGFSYLAVLFRLASLPQGVNFKQIFAIAVLC
                     GIGFTMSMFLASLAFDVSEAGESITALARLGILLGSAVSAILGYTLLRMATKQNNPEN
                     NATY"
BASE COUNT         2519 a         1889 c         1655 g         2489 t
ORIGIN      
        1 gaaataactt acttatagac tcagaaattt aagtgcgcgt attttacgct atttgtaata
       61 taattcatct tctggagtgt aaatttattg taaataatac gaggaatcta accgcttgtt
      121 ataacgcaaa acaccttgac ttaaatcaag ccactacctc attttgaggg gagatttata
      181 cgcagtgaaa ttttaaaata agctaaggtt tacggataat cgttcaacac tattgaatta
      241 tcactgtagt tatcgttaaa aaggtatgct tttatgaacc aattcttccc tctaatcttt
      301 agcttaactc tattttccgg cgtaactttg gcactagaaa aagcacctcg aactatgccg
      361 acagaaggaa taaataagcc cctacttgga caaattctat tctttgacaa tatgctgtca
      421 cacaacggtt cacaaaactg ctccagctgc cataatccgg attatgcctt tattgataac
      481 cgcactactc cggcaaaagg ggtggtttca caaggagata ctccaaccaa attcggcaaa
      541 cgcaactccc cgaccatgct ttatgcgaaa tactcgcctg agttccactt tgatgagcaa
      601 agtcaagaat atatcggcgg gcagttctgg gacggacgag ccaaaaatct cgccgagcaa
      661 gccggtatgc cgcctatcga cccaaatgaa atgggaatgc ctaacaaatt agaagtagcc
      721 aaaagattat ataatgtgcc gatgtatcag cgatttttag ggcaatttta cggtgaaaag
      781 gtatgggaaa gttatgaaag cgtttatgcg gcaatggaag atgcgattgc gacattccaa
      841 caagaaaaca aacttctcgc tccatttgat tcaaaatatg atctattttt aaaaggcgag
      901 gtacaattaa ctgcattgga agaaaaaggc agagctttat ttttcaataa agctcaaact
      961 aattgtagta gttgccacca attacagagt gctgcaaacc accaagaaga aacctttagc
     1021 aactatcact attacaatgt aggcgtaccc ggtaacccag acttgatagc ggttaacggc
     1081 ttcgcaccag acttcgtcga tttaggctta tttgaaaacc caaatgttaa aggtgatgaa
     1141 acacaaaaag gcaaattcaa aaccccaaca ttgcgtaatg ttgccgtgac tgcactttat
     1201 atgcacaacg gtgtgtttaa agaattaaag actgttttgc tgtttttaga cagctacaat
     1261 aacccggagc gaaaaatcaa ccctgaaacc ggcacggcgt ggcaacaagc agaatttgaa
     1321 cccactatcg cccaccaaaa actcaaagcc aaacccttaa gcgatgaaga aattgatgcc
     1381 ctcgaagcat ttttaaaaac cttgacggat gagcggtata gaaaataagg ctacaagcgg
     1441 taaaattttg gggaaatttt accgcttgcg tttaactacc gtgttagctc actttctaac
     1501 ttttccaatg cctgcaaatc ttcccaatct gcctctttct gttttaaaca tttacgctga
     1561 atatcaaatt tttcgtattg ctctctgaca aagctttcca tttgtttatg cgtgattgtt
     1621 ccaccatttc ctaatacaga aaagccgtta aactcaatca acatatctac atttcttcgc
     1681 cagaaaccta acgtaagatc ttggttgttc ttcacacgta attcggcact ttcaagaaag
     1741 atcattacca agcgatttaa agaatccagc tcattattat ctaaatagtt tttcgcaatc
     1801 acaatatcgc ctttacgcac gacttttcct ttccagctag ttaatcccat attcatctta
     1861 gtcgcatcag cacgttgaca aatcaactca gcggcagtgt gtttggttat tgcataaatg
     1921 agcttatttt gcgtttctgc aaaaaacatt tgcgtatttt tatctgtttt gtcgtaatcg
     1981 ctagagagtt tgaaaagctc tcgtactttt tgatagaaac gcaattcact tgcccgaatc
     2041 tcacgaatct gttcaagtaa ttcatcgaaa taatctaaac gaccctgcgg atttttcaaa
     2101 cgctcgctat caattaaaaa acctttttcg agataagtgc gtaatgtgct gtttgcccat
     2161 tttctaaact gtactgctct tgggctacga actcgaaacc ctatcgccag aatcatttca
     2221 agtgaataat gttttacttg ataattcttg ccatcttgag cagttatcaa gtaatccttg
     2281 ataactgaat tctcatctaa ctctttgtct tttaagatat ttttaatgtg ttcggatata
     2341 ttttgcacag aggtgtcaaa aagttctgca agttgatttt gtgtcagcca agcttcattt
     2401 tcaaccacta acaaggctac attgcttttg ccgtcttccg tattataaat aattaaatcg
     2461 ttcatatttt attctgttta ttggtagaga tattagtcgc tattttcaat aaaacatgta
     2521 taaatatcca gaattgattt tgaatttttc gatgcagatc acaaaaatag agagttatac
     2581 aaacaagcgg taaaattttg taaaaaattt accgcttgta aaggttactt atcagctaat
     2641 ttggcaagta gtttttgatg aataccgcca aaggctccgt tgctcatcac taagatgtga
     2701 tcggtgggtt tggcttcttt gacgactaga tcgactaaat catcaatatt tgccgaccaa
     2761 tacgccggtt gcgataacac attggtaatc tcgctcacac tccacgcaat aatatcaggc
     2821 tggtaaacaa acactgcttc ggcatcgtgt aggcttgggg ctatttcatc tttatggaca
     2881 cccattttca ttgtatttga acgaggctct aacactgcca aaatgcgttg atttttgccg
     2941 actttaccac gcagagcatc aatagtcgct tctatcgcag ttgggtggtg agcgaaatca
     3001 tcataaacgg taatgccgtt tacctcgcct ttcacttcca aacggcggtt agcattcaca
     3061 aacttgccta atgcctcgca agccccttgc acttcaaccc ctgcgtgatg agcggcggca
     3121 atcgccatca gagcattatg catattgtgc ttgccgataa tcgaccaatg cacctcgcct
     3181 tgtttaacgc ctaagtgaaa cacctcaaac tgtgagcaat cggcatttaa cggtttagca
     3241 aaccattcgt tctgttcgcc aataaattga agctggctgt aactgcccat ttttagtgta
     3301 tcttgcacgt tttgatcagc gattgccgac aaaatacagc cactttgtgg catcgtgcga
     3361 acaaggtggt ggaactggcg ttgaatcgct tttaaatcat caaaaatatc ggcgtggtca
     3421 aattccatat tattgataat cagcgttttc ggggtgtaat gcacaaattt agaacgctta
     3481 tcaaagaacg cagaatcgta ctcgtccgcc tcaatcacga agaatggact gttaccggca
     3541 cgagccgaaa tgccgaaatt accggcaaca ccgccaatta aaaagccggt atcaatccca
     3601 ttttggtcta aaatccacgc taacattcct gttgtggtag ttttgccgtg cgtgccggaa
     3661 accgccagca cccatttgtc tttaagcaaa tgatcgtgca accattgcgg gccggaggtg
     3721 taattgagtt ggttgtccaa cacatattcc acgcacggat taccacggct catcgcatta
     3781 ccaatcacca ctaaatcagg agccggttgc agttgagcaa catcgtaatt cgggataatt
     3841 tcaataccgt gagcagctaa aaaggtactc atcggtgggt aaacattagt gtctgagccg
     3901 gtcactttat agccaagctc acgggcaatc attgccacac cgcccataaa ggtgccgcaa
     3961 atgcctaaaa tatggatatg ttttttcatc tttttatatt taaaatgtta gaatcatccc
     4021 ctctcccctt gtgggagagg gatagatttt ttcttcgtgc agaagaaaaa atcagggaga
     4081 ggggtaagcg gtcattttta ggcttaatat tgtaagctcc ctctctctga aaaatatttc
     4141 tgaacgaaat atttttcggt ctcaatactc gctacgctcg ctcctgtgga gccgttggca
     4201 aagccaacgt tcaaatgtaa acatttgtcc cacaagggga gaggtgcgct ttagatgagg
     4261 cgtaccatcc ctcgttcaca tcaattaatc aatattcttc aactcttctt ccgaaagctg
     4321 ctcttgttgg ctttgctcaa cattgccatc tttcacttta aattcaaggt tttggaaata
     4381 agcttcacgg aaggtaacat aagggtcttg agcttgctct aacaacgcat cttgttctaa
     4441 taatttagaa cgggtatcta ccccttgtac cacaaattta gcgatagacc aaggtgaccc
     4501 aacataagtc agcaccgaat aaccggtttc caccgcacta ccaatacctt gacgaggtgt
     4561 ggttgctcca taagccggca tcataatata ggagccggaa ggcacgccgt gagcgcctaa
     4621 actctcacct aaaccacgtt gtccgttatt taaacgtaac ccctcatcta aactcgccca
     4681 gtcaattaac ccacctaaac caaacacagt gttgatccag aagcggttga agtgaaccat
     4741 cgctttttta ccttctcctt caattaagcg gtttacaaag ctcaccggct catcaaggtt
     4801 attagcaaca ttaattaagc ctgttttaac cgggcttggt acatactctt tccaaccttt
     4861 agctaccggt tttaacacat aagggtctaa atagtcgtaa ttgactttcc acatcgcacg
     4921 gttaaacccc tcaagtgggt cgtctcgcac gccggtttct gggttaatgc ttgatgaaca
     4981 agcggttaaa aatgccacac tgagtagcat aaacatcggt ttgaacgact tgagtttcat
     5041 aaattgaacc ttttgcagag aaatttgtcc attgtagcga agttgataaa aaatacaaaa
     5101 caaaaagccg ttttgcctct agaaatctag acgtctagat tgagatatag ttcgcttttg
     5161 attacaatac aggcaacatt taaggagcaa acaaatggca gactggaacg gcgaatatat
     5221 tagcccttat gccgaacacg gcaaaaaaag tgaacaagtc aaaaaagtga cggtatccat
     5281 tccaattaaa gtattggaaa ttttaactaa tgagcgaacc cgtcgccaaa tcaaaaatct
     5341 tcgtcacgca accaacagcg aattattgtg cgaggctttt ttgcacgcct ttaccggtca
     5401 gcctttgccg aatgatgaag acttgatgaa agacagggca gatgaaatcc ctgaagcggc
     5461 aaaacaaaaa atgtgtgagc tgggaataga cccggacgaa tggcaatatt agacacacct
     5521 tgcaactgcg aggttctttt tatgttttaa ggagacttta tgtatcaaca attaacaaca
     5581 gaagcacgtt gggctgtgtt gcttacgctg atttatctgc tcggctgggt aggttttgct
     5641 tacttttcac cgcaaggcag aggcattttc ggctttccga tttggtttga actttcttgc
     5701 attttcttac ccgtactatt tagcttagtt gccaccattg tcgtcaaaaa agtgtataaa
     5761 aatatcgatc ttgaggtgaa taatgaatag tgaaatgatt ttacccctga ttgcttattt
     5821 aatttttgtg tttggcgtag cgttttatgc ttaccgtaaa cgccaaggcg gaagctttat
     5881 ttcggaatac tatatcggtg ggcgttcaat gtcgggcttt gtgttagcaa tgacaaccgc
     5941 cgccacttat gtgggggcaa gttcctttat cggtgggccg ggtgctgcct ataaatacgg
     6001 tttaggctgg gtgttactgg caatgattca agtgcccgca gtgctactct ctcttggagc
     6061 attaggcaaa aaatttgcct tacttgcccg caagcataat agtgtcacca ttaacgatat
     6121 gttattagcc cgttaccaaa acaaatttgt ggtttggatt gcaggactag ccttattact
     6181 ttcgtttttt gcgatgatga cggtgcaatt tatcggtgcc ggtcgcttac ttgaaaccac
     6241 actcggaatc ccttaccaaa cagcggtaat gatttttgcc atcacagtcg ggctttatac
     6301 ctttatcggc ggttttagag cggtggtgct aaccgatact attcaaggtt tagtaatgat
     6361 agtcggcaca agcattttac tcgctggtgt gatttatgcc acaggcggag tggaaaatgc
     6421 aatgcacacc cttgaagcca ttaacccaca acttaccacc ccttacggca ttgacgaacg
     6481 cccgcttgat ttcaccttta tgacttcatt ttgggtattg gtttgctttg gtttaatcgg
     6541 cttaccgcat attgcggtca gaagtatggc atgcaaaaac agccaagcct tgcaccgagc
     6601 aatgattatc ggcacggtag tcattgcaat tctaatgttt ggaatgcacc tttcaggcgt
     6661 attaggcaga gcggttgtgc ctgatctcaa agtgccggat caagtcattc caacactaat
     6721 ggtacaagtg ctaccaccgg tggttgccgg tattttcctt gccgccccaa tggcagcgat
     6781 tatgtcttcg attgactcaa tgttgattca gtcgtcttca accttaatta aagatctcta
     6841 tctggcaatt aagccgaatg ccattaataa cgagaaaaaa atcaaattgt tctccactat
     6901 tactactctt acttttacgg tattactcgt gtttgccgcc ctaaccccgc ctgagatgtt
     6961 aatttggctg aacctactct ctttaggtgg tttagaagcg actttcctat gggtaattgt
     7021 actagggttg tattgggaaa aggcaaatgc caccggtgct atttactcaa tgctggcagg
     7081 cttaagttgt tatgtattaa tgactacctt taagattgct atttttagct tccacgccat
     7141 tgtgccttca ctgctaattg gtttgattgc tttcttaatc ggcaaccgat ttgctcctaa
     7201 ggtgtaaaat aaaataggca tagtggtatg gaagttcact atgcctattt tgcaaaattt
     7261 ccgctcaatt caaccgcttg tagttaataa gtggcattat tttctggatt attctgctta
     7321 gtcgccattc taagtaacgt ataacctaaa atcgctgaaa ccgcagagcc gagtaaaata
     7381 cctaatcgag ctagagcggt aatactttcc ccagcctcac ttacatcaaa ggcaagactt
     7441 gctaagaaca tcgacattgt aaaaccgata ccgcatagca ccgcaatggc aaaaatttgc
     7501 ttaaaattaa ccccttgcgg caggctggca aggcggaata atactgctaa ataactaaaa
     7561 ccgaatacac ctaaggtttt gccgataatt aagccgagcg caatacctaa cggcaatggt
     7621 gatgagagtt gctccattcc catttctaat aagtaaacgc cggcattagc gaaggcgaag
     7681 atcggtaaaa tcatataagc acaccaaggg gcaagaatat gttctagctc ctctgccggt
     7741 cgttccccat gtttaccttt cagcggtacg cagaagccga tgatcacgcc ggcaagggta
     7801 gcgtgaaccc ctgattttaa aacggaagtc catagcacta agcctactac caaataagca
     7861 cagagtgagg tcactttaaa gcggttaagt agcactaagc cgataatcgc aatcgcggca
     7921 atagttaagg cttgcacact gatttcttgc gaatagaaaa tggcgattac cacaatcgct
     7981 cctagatcat caataatcgc taatgccaac aaaaatgcct ttaaagccaa tggcacacga
     8041 gtgcctaata acgccacaat gcccaccgca aaggcaatat cagttgccat tggaattgcc
     8101 caaccattat gatattcagg gtggttttga ttaatcgccc aataaataat cgctggaaca
     8161 atcataccac ctactgctgc tatcgcagga aaaatagctt tcttataact tgaaagcgaa
     8221 ccttcaaaca actctttttt tacttccagc cccaccaacg taaaaaacac tgccattaag
     8281 ccatcgttaa cccagagtaa tagcggttta ttcaaggcaa attcaccaaa ctgtacacta
     8341 acaatcgttt gtaaaaactc aaaatagaaa tcacttaagg aggtgttggc gcacacaatt
     8401 gctgcaaaag caaaaaccag aagcaatacg cctcctgagg cttcagattt taaaaattct
     8461 tttaattttt ccacttaact ttctcctatt aatatttgtt tattgtattt tcatttctat
     8521 acattccatc ttttaccaaa aattttgcga ag
//
LOCUS       DANLUQ010000111         8362 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.112, whole genome shotgun sequence.
ACCESSION   DANLUQ010000111 DANLUQ010000000
VERSION     DANLUQ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8362
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.112"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            177..554
                     /locus_tag="PXB27_001875"
     CDS             177..554
                     /locus_tag="PXB27_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HDL5346634.1"
                     /translation="MEYQFTHSIHGVVAKCSMDHEAFARWLNAEITENPSQLAPIFAE
                     IEKCRAAFPNHYECVFEGREYSLYFDYDEVMAKANNLDAAFDDEEMEEGFQFYNEESI
                     AFCGLDDFEKFLKAYQHFVQTYH"
     gene            643..1230
                     /gene="yihI"
                     /locus_tag="PXB27_001876"
     CDS             643..1230
                     /gene="yihI"
                     /locus_tag="PXB27_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HDL5346635.1"
                     /translation="MSRTKKTRRITDIMPARKTDKPKQPMPKLGGGKNRKLTRYELDA
                     QAREEKRKRKHKGLPTGSRNADPAEQKKAVVKEVKDPRIGSRKKVPLMVEFVNQPEKG
                     RTIKAVPVEPIKPTLSPEQELEQLENNECLNQLLDDLEAGKTLSAEDQKFMNECLDRI
                     DELMTELGIEYEDEEGDNGDALLRQFETMDINKFR"
     gene            1244..1711
                     /locus_tag="PXB27_001877"
     CDS             1244..1711
                     /locus_tag="PXB27_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2489 domain-containing protein"
                     /protein_id="HDL5346636.1"
                     /translation="MLRIFLMVLAALILISLGGYALHLMLKVRAQKKHEQALIEQAKQ
                     AQKDRYIRILDSIEVIARAMISEQCDFSEGVLRLKPLLDVLGRKLSDYTAMWALYGVV
                     ENMPILDERKNLKRNERMKLDLEREAKEAELESDIKNECYQLLKDIEEMRKAI"
     gene            1711..3078
                     /gene="hemN"
                     /locus_tag="PXB27_001878"
     CDS             1711..3078
                     /gene="hemN"
                     /locus_tag="PXB27_001878"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDL5346637.1"
                     /translation="MSEIIWDLALIQKYNHSGPRYTSYPTALEFNENYTNEDFIRAAA
                     RYPERPLSLYVHIPFCHKLCYFCGCNKVITRHRHKVEIYLDYLEREIKTRAPLFQNRL
                     VTQIHWGGGTPTYLDEDQSARLMQMLRANFDVSDDAEISIEMDPREIELSMLDHLRAI
                     GFNRLSMGIQDFDKEVQRLVNREQDEEFIFALMKRAKELGFVSTNVDLIYGLPKQNVE
                     SFMYTLKRVVELNPDRMSVFNYAHLPSRFAAQVKIKEELLPAPETKLTILQNSIEFLG
                     KNGYKFIGMDHFAKPDDELAIAQEKGILHRNFQGYTTQEECDLLGMGVSAISLLGDTY
                     AQNRKELQQYYADVETNGIALHKGIALTQDDCIRRDVIKALICNFKLDFATIEKQYGI
                     DFKRYFAEDLAFLAPLAEDELLTMNDDSIVVSPRGRLLIRNICLCFDVYSRQLARRQQ
                     FSRII"
     gene            3152..5485
                     /gene="lptD"
                     /locus_tag="PXB27_001879"
     CDS             3152..5485
                     /gene="lptD"
                     /locus_tag="PXB27_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HDL5346638.1"
                     /translation="MKQRYSLLSLAVMAACYSNSAAADLKQQCLLGVPHFQGEVVEGD
                     QLRMPVTIEADSAVINQPRDATYTGDVTIKQGNRSIWADQVRVEQDGENARMAYLQGN
                     FNYQDNLIQATGSDATINLLSKDANLANANYQLIGRQGRGTAESGEFGNSKHTLKNAS
                     FTSCLPNDNSWQIEANEMIQHVDEEYAEMWHARFKVLGMPVFYSPYLQFPIGDRRRSG
                     LLIPSFERSSKNGWTYSQPFYWNIAPNMDSTITPTYYSRRGWQISPEFRYLTKIGQGL
                     VATEYMAKDRLSEYQPGNDDRKRYLFHWRHAMSFLTDWRFSVDYTKVSDRRYFSDFDS
                     DYGSSTDGYATQNFKLGYYQPNYNISISGKKFQTFDELDIGPYRVLPQIDFNYYKNDL
                     IKGGDFKFFAQAARFENDSKLMPKAWRFHAEPTLNFPLANRYGSLNLETKLYATYYQQ
                     TKGSESTEEIKKSVSRVLPQIKLDFKTVLESDKQLFSGYTQTLEPRVQYIYRPYKDQS
                     DIGSKAHRSVSLGYDSALLQNDYFSLFNDRRYSGLDRISSANLVTAGGTTRFFKEATG
                     EEVFNFSLGQTYYISPSKIDDTSDNSTKGHTTSWAAESNWKFHKNWNWHGSYQYDTRL
                     NQTALANMSLQYKPQSDKLIQLNYRYASKDYINQNLNTNKYGQSIKQVGGVLGWELTD
                     KVSVMTSYYHDLALKKPVEGQISLNYNTCCWAANVYVARRLVSTPVGSSDTINDFYYD
                     NKFGINFELRFGTNYSNGVTRMLKRGIIPYTEPYSIN"
     gene            complement(5627..8266)
                     /gene="ppc"
                     /locus_tag="PXB27_001880"
     CDS             complement(5627..8266)
                     /gene="ppc"
                     /locus_tag="PXB27_001880"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HDL5346639.1"
                     /translation="MNQQYSTIQHNIHMLGEFLGETIRDAQGNYILELIESIRVLSRN
                     SRAGDENAREQLLDKLANISNDDVLPVARAFSHFLNLTNIAEQHQTVSRHHIDESLGS
                     RSLDSLFLRLKSQNIPVEKVIKTVESLMIDLVLTAHPTEVTRRSLGHKHVEINKCLNL
                     LEHDDLTEAESYKIKRRLMQLIALAWHTNEIRTQRPTPVDEAKGGMAVIENSLWKAVP
                     EFCRQLNFYLEKHFGVQYPVNLAPVRFSSWMGGDRDGNPFVTAETTRRVLRMNRWKAT
                     ELFLQDIKNLAEELSITQCTPAFRAKYGNHLEPYRVLLKVLRQKLINTEIYYAELLEG
                     KISTIDESDILTSDQQLWEPLFDCYESLQACGMRIIANGALLDCLRRINCFGLGLSRL
                     DIRQESTRHEMAIAEITRYIGLGDYAHWTEDDKQAFLVRELSSRRPLIPTNWTPSPET
                     KEVLDTCKIVAEQPEGVISAYVISMAREASDVLSVHLLLKEAGCKFTIPVAPLFETLG
                     DLDHSEKVMTDLFNIGWYRGVINNYQMIMIGYSDSAKDAGMLAAGWAQYRAQEALVNL
                     CEKYNIELTLFHGRGGTIGRGGAPAHAALLSQPPRSLKNGLRVTEQGEMIRFKLGLPA
                     VAVESLELYASAILEANIFPPPEPKQQWRDVLAKASDISCQIYRDMVRGELDFVPYFR
                     SATPEQELSRLPLGSRPAKRNPKGGVESLRAIPWIFAWMQNRLMLPAWLGAGQALQQL
                     IDEGNETLLKEMCNEWPFFSTRIGMLEMVFSKADIWLAEYYDQRLVEPKLHRLGNMLR
                     TQLQADLKTVLALAHDGQLMADLPWIAESIALRNIYTDPLNLLQVELLDRIRKQGENA
                     DPVLEQALMITITGIAAGMRNTG"
BASE COUNT         2546 a         1611 c         1830 g         2375 t
ORIGIN      
        1 gtagttcctt tttgatgtat caatactaaa aataacctgt attattctag cagatttctg
       61 ggtaatcgag gagttgataa catttaaaaa ttagcgtttt gcaaaatttt ccaaaaaaag
      121 accgcttgtt aggctataat agccaaccaa acagaccaat tcaaaggaga atcggtatgg
      181 aatatcaatt tacccatagt attcacgggg tggtggcaaa atgttcgatg gatcacgagg
      241 cgtttgctcg ttggttgaat gccgaaataa cggaaaatcc tagccagtta gcccctattt
      301 tcgctgaaat agagaagtgt cgagccgcat ttccgaatca ttatgaatgt gtgtttgaag
      361 ggcgggagta tagtctctat tttgattatg atgaagtgat ggcaaaagca aacaatttag
      421 atgctgcttt tgatgatgag gaaatggaag aaggctttca attttacaat gaagagagca
      481 tcgcattttg tggcttagat gactttgaga aatttttgaa agcctaccaa cattttgtgc
      541 aaacttatca ttgattgaaa tgattaattg aaaccaaagc tattaaggga atctaattcc
      601 ttgtagaata gccgtttata gcaattttaa gatattaaat ttatgagtcg tactaaaaaa
      661 acacgtcgta ttaccgacat tatgccggca agaaagaccg ataaacctaa acagcccatg
      721 cctaaattag gtggggggaa aaatcgcaaa ctgacccgtt atgagttaga tgctcaagcc
      781 cgtgaggaaa aacgtaagcg taaacataaa ggcttgccga caggctcacg caatgctgat
      841 ccggcagagc agaaaaaagc agtggtgaaa gaggtgaaag atccgcgtat cggtagccgt
      901 aaaaaagtgc cgttaatggt ggaatttgtg aaccagccgg aaaaagggcg gacgattaag
      961 gctgtgccgg ttgagccaat caaaccaacg ctttcaccgg aacaagagct ggagcagtta
     1021 gaaaataacg aatgtttaaa tcagttgtta gatgatttgg aggcgggcaa aaccttatcc
     1081 gcagaagatc agaaatttat gaatgaatgt ttagatcgca ttgatgaatt aatgactgag
     1141 cttggcatcg aatatgaaga tgaagagggc gataatggcg atgcgttatt acgccaattt
     1201 gaaacgatgg atattaataa attccgctag gtagccgatt atgatgctaa gaatattcct
     1261 gatggtgctt gccgctttga ttttaatttc gctgggcggg tatgcactgc acttaatgct
     1321 gaaagtgcga gctcagaaaa agcacgaaca agcactgatt gaacaggcaa agcaagcaca
     1381 aaaagatcgt tatattcgga ttttagacag cattgaagtt attgctcggg caatgatttc
     1441 agaacagtgc gatttttcgg aaggcgtttt acgtttaaaa ccgctattag atgttctcgg
     1501 cagaaaatta agcgactata cggcgatgtg ggcgttgtat ggcgtggtag aaaatatgcc
     1561 gattttagat gaacgcaaaa atttaaaacg taacgaaaga atgaagttgg atttagagcg
     1621 ggaagcaaaa gaagcggagc ttgaatcaga catcaaaaat gagtgttacc aattattaaa
     1681 agatattgaa gaaatgagaa aggcaatcta atgtcagaaa ttatttggga tttagccctt
     1741 attcaaaaat ataaccattc agggcctcgt tatacctctt acccaacagc gttggagttt
     1801 aacgaaaatt acaccaatga agattttatt cgtgctgcag ctcgttaccc tgagcgtccg
     1861 ctttcgcttt atgtgcatat tccgttttgc cataaactgt gctatttctg tggctgtaat
     1921 aaagtgatta ctcgtcatcg ccataaagtt gaaatttatt tagattattt agagcgtgaa
     1981 attaaaactc gcgcacctct gtttcaaaat cgtttagtga cccaaataca ctggggtggc
     2041 ggtacgccga cttatttaga cgaagatcaa tcggcacgtt taatgcaaat gctacgagcc
     2101 aattttgatg tgagcgatga tgcggaaatc agcattgaaa tggatccgcg tgaaatcgaa
     2161 ttaagtatgt tggatcacct gcgtgctatc ggttttaacc gtctgagtat gggtattcag
     2221 gatttcgata aagaagtgca acgtttggtg aatcgtgagc aagatgaaga gtttattttt
     2281 gccttgatga aacgggcaaa agaacttggt tttgtttcaa ctaatgtgga tttgatttac
     2341 ggcttgccaa aacagaatgt ggaaagcttt atgtacacct taaaacgtgt ggtagagcta
     2401 aaccctgatc gtatgagcgt atttaactat gcccacttgc caagccgttt tgctgcacaa
     2461 gtgaaaatta aagaagagtt gttgccggct ccggaaacca aattgacgat tttgcaaaat
     2521 tccatcgaat ttttaggcaa aaacggttat aagtttatcg gtatggatca ctttgctaaa
     2581 cctgatgatg agttagctat tgcacaagaa aaaggcattt tacaccgtaa cttccaaggc
     2641 tataccactc aagaagagtg tgatttattg ggaatgggcg tatcggcaat cagcttactc
     2701 ggcgatactt acgcccaaaa ccgcaaagaa ttacaacaat actatgctga tgttgaaacc
     2761 aacggtattg cgttgcataa aggcattgcg ttaacccaag atgattgcat tcgccgtgat
     2821 gtgataaaag ccttgatttg taatttcaaa ttagattttg ccaccattga aaagcaatat
     2881 ggtattgatt ttaaacgtta ttttgccgaa gatttagctt tcttagcgcc gttggcggaa
     2941 gatgaattat taacgatgaa tgacgacagc attgtggttt ccccacgagg gcgtttattg
     3001 atccgtaata tttgcttatg ctttgatgtc tattcaagac aattagcgag aagacagcag
     3061 ttttcccgta ttatttaatg agcaagcggt cgattttggt aaaaattttg caaaaacaga
     3121 ccgcttgttc ccggcttata aaggaaaaat gatgaaacag cgttatagct tactttctct
     3181 tgctgtgatg gcagcttgtt atagcaacag tgccgcagct gatttaaagc aacaatgttt
     3241 acttggcgtg ccgcattttc agggcgaagt ggtagagggc gatcaactta gaatgcctgt
     3301 taccattgag gctgacagtg cggtcattaa ccagcctcgt gatgccacct acaccggtga
     3361 tgtaacgatc aaacaaggta accgttcgat ttgggcggat caggtgcggg ttgagcaaga
     3421 tggcgaaaat gcccgaatgg cgtatttgca gggtaatttt aactaccaag ataatttgat
     3481 tcaggcaacc ggttcagatg cgaccatcaa tctgctttcc aaagatgcga atttagctaa
     3541 tgctaattac caattaattg gcagacaagg gcgtggcacg gcagaatccg gtgaattcgg
     3601 caacagcaaa cacacgctga aaaatgccag tttcacttcg tgcttaccga atgataattc
     3661 gtggcaaatt gaagcgaatg aaatgattca gcacgttgat gaagaatatg cggaaatgtg
     3721 gcacgcccgt tttaaagtgc tgggaatgcc ggtattttat tcgccttact tacaatttcc
     3781 gattggcgac cgccgccgta gtgggttatt aattccaagt tttgagcgtt caagcaaaaa
     3841 cggctggaca tattcgcaac cgttttattg gaatatcgca ccgaatatgg actcgaccat
     3901 cactccaact tactattcac gccgtggttg gcaaatcagc ccggaatttc gttatttaac
     3961 gaaaatcggg caaggcttag tggcaaccga atatatggcg aaagatcgtt taagcgagta
     4021 ccaaccggga aatgatgatc gtaaacgcta tttattccac tggcgtcacg caatgagttt
     4081 tttaaccgat tggcgtttct cggtcgatta caccaaagtg agcgacagac gttatttctc
     4141 tgattttgat tcggattatg gtagcagtac tgatggctat gcgacgcaga attttaaatt
     4201 aggctactat cagccgaatt ataatatctc aatttcaggt aaaaaattcc aaaccttcga
     4261 tgagttggat atcgggcctt atcgcgttct gccacaaatt gattttaact attataaaaa
     4321 cgatctaatt aaaggcggag atttcaaatt ctttgcccaa gcagcccgtt ttgaaaatga
     4381 tagtaaatta atgccaaaag cgtggcgttt ccacgctgag ccaacactga atttcccatt
     4441 agcgaaccgc tatggtagct taaatttaga aaccaagctc tatgcaacct actatcaaca
     4501 aacgaaaggt agtgagagta cggaagaaat taagaaaagt gtaagtcgtg tactgccaca
     4561 gattaagctc gattttaaaa ccgtgttgga atccgataaa cagcttttct ctggctatac
     4621 tcaaacgctt gagcctcggg tgcaatatat ttatcgccca tataaggatc aaagcgatat
     4681 tggctcgaaa gcacatcgaa gtgtgagctt aggctatgac tctgcattat tgcaaaacga
     4741 ctatttctca ctgtttaatg acagacgtta tagcggttta gatcgtattt cgtcagcaaa
     4801 tttagtcacc gcaggtggca caacccgttt ctttaaagag gcaaccggtg aagaggtgtt
     4861 taatttcagc ttaggacaaa cttattatat aagcccgtct aaaattgatg atacctcaga
     4921 taacagcact aaaggtcata cgacttcatg ggcagcggaa tctaactgga aattccataa
     4981 gaattggaat tggcatggta gctatcaata tgatactcgt ttaaaccaaa cggcgttagc
     5041 gaatatgtca ttgcaataca aaccacagtc agacaaattg attcagctta attaccgcta
     5101 tgcaagtaaa gattacatca atcaaaactt gaacacgaat aagtatgggc aatccattaa
     5161 acaagtgggc ggtgtgctag gttgggaatt aaccgataaa gtgtcggtaa tgaccagtta
     5221 ttaccacgac cttgcattga aaaaaccggt ggaagggcaa atttcgctta actacaacac
     5281 ttgctgttgg gcggcaaatg tgtatgtggc tcgtcgctta gtttcaacgc cggtaggttc
     5341 atctgatacg atcaatgatt tctactatga caataaattc ggcattaact ttgagctacg
     5401 ctttggcaca aactacagca atggtgtaac ccgtatgcta aaacgaggta ttattcctta
     5461 caccgagccg tatagcatta attaacaaag taaaaacaaa taagtaaaaa tacaaatcgg
     5521 ggcgaaaaat atttgccccg atttttttat atttggctga taagcggtta aattctgttg
     5581 gaattttgca aatcttccgc taaattcaac cgcttgccat cgaaacttaa cctgtatttc
     5641 gcatacctgc ggcaatacct gtgatggtga tcatcaatgc ttgctctagt acaggatcag
     5701 cattttcacc ttgtttgcga atcctatcta acaattctac ctgaagtaga ttgagcggat
     5761 cggtataaat attgcgtagt gcaatcgatt ctgcgatcca aggtaaatcg gccattaatt
     5821 gtccatcgtg agccagtgca agtacggttt taagatcagc ctgtagttgc gtacgcaaca
     5881 tattgcctaa acggtgtagt tttggctcga ctaggcgttg gtcgtaatat tccgccagcc
     5941 aaatatcagc tttactaaat accatttcca gcattccgat acgggtcgag aaaaacggcc
     6001 attcgttaca catttctttt agtaaggttt cattaccttc atcaatcaac tgttgcaatg
     6061 cttgccctgc acctaaccaa gcaggtagca ttaagcggtt ttgcatccaa gcgaaaatcc
     6121 aaggaattgc acgcaaactc tccactccgc cttttggatt acgttttgca gggcgtgagc
     6181 caagcggcag acgggaaagt tcttgctctg gtgtagcaga gcggaaatac ggtacgaagt
     6241 ctagctcgcc acgcaccata tcacggtaaa tttggcaaga aatatcggag gcttttgcta
     6301 gcacatctcg ccattgctgc tttggctccg gtggtgggaa aatattcgct tctaaaatcg
     6361 cactggcata gagttctaaa ctttcaactg ccacggcagg taagcctaat ttaaagcgga
     6421 tcatttctcc ttgttcggtc actcgtaagc catttttgag tgagcgaggt ggctgagaaa
     6481 gtaaggctgc gtgagccggt gcgccaccac gcccgatagt accaccacga ccgtggaata
     6541 aggttaattc aatattatat ttttcacata aattaaccag agcttcttgc gcccgatatt
     6601 gcgcccagcc ggcagcaagc attccggcat ctttggcgga atcagagtag ccgatcataa
     6661 tcatttggta attgttgatg acgccacgat accagccgat attaaataaa tcggtcatca
     6721 ctttttcact atgatctaaa tcgcctagcg tttcaaataa tggggcaacc ggaatagtaa
     6781 atttgcaccc cgcctctttg agtaaaaggt gaaccgagag tacatcagag gcctctcttg
     6841 ccattgaaat gacataggct gaaatgacac cttcaggttg ctcggcaaca attttacaag
     6901 tgtctaacac ttctttagtt tcagggcttg gagtccagtt ggttggaatc aatgggcggc
     6961 gagaactgag ttcacgcact aagaatgcct gtttatcatc ttccgtccag tgagcataat
     7021 cgcctaagcc gatatagcgg gtaatttctg caatcgccat ttcgtggcgg gtactttctt
     7081 ggcgaatatc taagcgtgat aaacctaaac caaaacagtt tatacgacgt aagcaatcta
     7141 gtaatgcacc atttgcaata attcgcatac cacaggcttg taatgactca tagcaatcaa
     7201 aaagcggttc ccacaattgc tgatctgaag ttaaaatgtc actttcatca atagttgaaa
     7261 ttttaccttc caataattcc gcatagtaaa tttcggtatt aattaatttc tggcgtaata
     7321 cttttagtaa tacgcgatat ggctctaaat ggttgccata tttagcacga aatgcagggg
     7381 tacactgtgt aatcgataat tcttcagcaa gatttttaat atcttgtaag aacaattccg
     7441 tggctttcca acggttcatt cttaatacac ggcgagttgt ttctgccgtg acgaacgggt
     7501 taccgtcacg gtctccgccc atccaagaag aaaaacggac aggggctaaa tttaccggat
     7561 attgcacacc aaagtgtttt tctaaataga agttaagctg acggcaaaat tccggcacgg
     7621 ctttccataa actattttca atgactgcca taccgccttt ggcttcatca acaggggttg
     7681 ggcgttgggt tctgatttca ttagtatgcc aagcaagggc aatcagttgc attaaacggc
     7741 gcttgatttt atagctttct gcctcagtta aatcatcatg ttccaacaaa ttcaaacatt
     7801 tattgatttc aacgtgttta tgccccagcg aacggcgggt tacttccgtt gggtgagctg
     7861 ttagcactaa atcaatcatt aggctttcca cggttttaat cactttttca acggggatat
     7921 tttgtgattt tagacgaagg aacagagagt ctaatgagcg actaccaagt gattcatcaa
     7981 tatgatggcg agaaacagtt tgatgttgtt cagcaatatt tgttagattt agaaaatggc
     8041 taaatgcacg tgcaacgggt aagacatcat cgttagaaat attggctaat ttatctaata
     8101 gctgttcacg ggcgttttca tcgccggcac gagaattgcg agataacaca cgaatgcttt
     8161 cgatgagttc tagaatgtaa tttccttgcg catcacgaat cgtttctccc aaaaattcac
     8221 ctaacatatg gatattatgc tgaattgttg aatattgctg gttcataaaa aattcctatg
     8281 gtaaacataa attaaattag gtctaaatat gccgaaaatc gctgaaaata gcaaatagat
     8341 ttaatttaat ttttgaatta aa
//
LOCUS       DANLUQ010000112         8349 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.113, whole genome shotgun sequence.
ACCESSION   DANLUQ010000112 DANLUQ010000000
VERSION     DANLUQ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8349
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.113"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(356..2026)
                     /gene="glnS"
                     /locus_tag="PXB27_001881"
     CDS             complement(356..2026)
                     /gene="glnS"
                     /locus_tag="PXB27_001881"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HDL5346640.1"
                     /translation="MSEEILTAETGTRANFITHIIDEDLASGKHNNVYTRFPPEPNGY
                     LHIGHAKSICLNFGIAQDYNGLCNLRFDDTNPVKEDVEYVDSIKQDVEWLGFKWEGEP
                     RYASDYFDQLYGYAIELINKGLAYVDELSPDEMREYRGTLTEPGKNSPYRDRSVEENL
                     ALFEKMKNGEFAEGKACLRAKIDMASPFIVMRDPVLYRVKFAHHHQTGDKWCIYPMYD
                     FTHCISDAIERITHSLCTLEFQDNRRLYDWVLDNISIERPLPHQYEFSRLNLEGTLTS
                     KRKLLKLVTDGIVDGWNDPRMPTISGLRRRGYTPASLREFCRRIGVTKQDNVVEYSAL
                     EACIREDLNENAPRAMAVINPVRVVIENFGEKEILKAPNHPNRPELGERDLPFTREIY
                     IDEADFREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDASGRISTIFCTYDPDTLGK
                     NPADGRKVKGVIHWVSAEDNKPAEFRIYDRLFTVPNPGAEEDINAVLNPESLVVKHGF
                     VESSLAHAKPEQDYQFEREGYYCLDSKDGSADNLVFNLTVSLKESVAF"
     gene            complement(2187..2630)
                     /locus_tag="PXB27_001882"
     CDS             complement(2187..2630)
                     /locus_tag="PXB27_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HDL5346641.1"
                     /translation="MNLQQIQDRLALKDLVDTFSNLADEKKVAEQMPLFTQDAVVNTY
                     IGGKLVFEMHGVAEIEQVFTTFLTPFHSVYHLNGQHTVPFIDENNATAINYCAVKLVE
                     TKEGKEILHDHSVRYADTYVKQEGKWRIAKRIANFMISESREIRQ"
     gene            complement(2681..3256)
                     /locus_tag="PXB27_001883"
     CDS             complement(2681..3256)
                     /locus_tag="PXB27_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDL5346642.1"
                     /translation="MKQILILGASGSLANVVIPELLKDNNVQLTLFARRPQSVAKFET
                     ERVKIVQGDVLDFHALTQAMQGVDAVYAGLAGDLKAMAGNVVNAMNEAQVKRLVWISS
                     MGIYGETGEDHGAILEPYRQSAAVIEASELDYTVVRPGWFTNGSEVQYQLTHKGEPFK
                     GHSVSRKSIADFVARILRENSEIRESVGIAA"
     gene            3363..4253
                     /locus_tag="PXB27_001884"
     CDS             3363..4253
                     /locus_tag="PXB27_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL5346643.1"
                     /translation="MKENLNDLRAFLLVAQTSSFTKAAAQMGVSQSALSHSIRGIEER
                     LQIKLFHRTTRSISTTEAGERLYQRLLPLFDEIDQEVNGLSEFRNALKGGLRINGNEH
                     VFRDVLADKFAEFVKRYPEVALELVAEDRFVDIVAERFDAGIRLGADVAKDMIALRIS
                     PDVKMTAAASPNYLAQHGTPKTPFDLTEHHCLKHRLATLGGILAWEFRDPITKKIVKF
                     QPQGNLTANNGFLLQHYAKQGLGILWSPQDSIQTELDNGSLVPILTDWAMQYEGYHLY
                     YPNRRQNSPLLKALIDVLRG"
     gene            complement(4320..4886)
                     /gene="efp"
                     /locus_tag="PXB27_001885"
     CDS             complement(4320..4886)
                     /gene="efp"
                     /locus_tag="PXB27_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDL5346644.1"
                     /translation="MASYSTNDFKKGLKFIQDGEPCVIVENEFVKPGKGQAFTRTKIR
                     KLISGKVLEINFKSGTSVEAADVVDSNYNYSYSDGDFWYFMHPETFEQISVDEKALGD
                     NVKWLVDNAECIVTLWNGSAIAVTPPNFVELEIVETDPGLKGDTAGTGGKPATLSTGA
                     VVNVPLFVQIGEVIRVDTRSGEYVSRVK"
     gene            5053..5622
                     /locus_tag="PXB27_001886"
     CDS             5053..5622
                     /locus_tag="PXB27_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346645.1"
                     /translation="MKKLITLSAILAVSVATVAEAKRGGGSFRSVSPSKAVATKPVAS
                     QKSTTNQQTQQKDATFANTPNSATAQPQVRQGSTMANVAMGAAAGYILGDMMSSNAHA
                     NQPSQPADANAQAVNSAQNVANGAVPAIAEFKSIGGQIDPFLVEKTDGYRRYCISGVQ
                     YLVAAQGVQTSPTVMVNPNGTPLQCNLIP"
     gene            5676..6668
                     /gene="epmB"
                     /locus_tag="PXB27_001887"
     CDS             5676..6668
                     /gene="epmB"
                     /locus_tag="PXB27_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HDL5346646.1"
                     /translation="MQPIQIKPLWLTELAQAFNNPIDLLQFLELNPNDFEGDIAARKL
                     FALRVPRMFAEKMEKGNPNDPLFLQAMSLQAEFIEAEGFVVDPLEEQQSPAPNILHKY
                     HNRLLFMLKNSCAINCRYCFRRHFPYEEVKSGKAVWQQGLTYIAEHPELEEVILSGGD
                     PLMAKDQDLDWILTQLEQISHIKTLRIHSRLPVVIPNRITTELCERLSKSRLNVVLVT
                     HINHANEIDAVFANKMAQLKKAGVVLLNQSVMLKGVNDNAQTLKRLSDKLFEYGILPY
                     YLHLFDKVAGASHFYIEDEQAGEIYRELQRITSGYLVPKLAREIAKEPNKILKG"
     gene            complement(6768..8171)
                     /gene="asnS"
                     /locus_tag="PXB27_001888"
     CDS             complement(6768..8171)
                     /gene="asnS"
                     /locus_tag="PXB27_001888"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDL5346647.1"
                     /translation="MSKFPTVSEILSGKIAVGEEATVRGWVRTRRDSKAGLSFLSVYD
                     GSCFDPIQAIINNDLPNYNAEVLHLTAGCSVVVTGKVVESPAEGQAVELQASKVEVLG
                     WVEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHCLAQAIHRFFHEQGFYW
                     VATPVITASDTEGAGEMFRVSTLDLENLPRGENGKVDFNQDFFGKESFLTVSGQLNAE
                     TYACALSKVYTFGPTFRAENSNTTRHLAEFWMMEPEVAFADLNDNAKLAEDMLKYVFK
                     AVLAERKDDMEFFVKHVDKEAISRLENFINSPFAQVDYTDAIEILLKSGKEFEFPVSW
                     GIDLSSEHERFLAEEHFKSPVVVKNYPKDIKAFYMRLNDDGKTVAAMDVLAPGIGEII
                     GGSQREERLEVLDQRMNEMGLNPEDYWWYRDLRKYGTVPHSGFGLGFERLIVYVTGLQ
                     NIRDVIPFPRAPRNANF"
BASE COUNT         2396 a         1784 c         1732 g         2437 t
ORIGIN      
        1 aagacaatgc tatcataaaa gagcatcact tttggtttcc aaagattata gaaagctcaa
       61 ataatacact acctcataat ttaaaatgaa acgtttcaat tggaaaaagt ggattttgtg
      121 actgagatca caaatttaaa cattggtctg tatttttgag agattttgtt tgaaactgcc
      181 ggcaaattcg cttaacctta aggtaacaag aataagtgtt gtacctagca acctaacaca
      241 taaatttgct ttccgagtgg ccccaagaat tggggctttt tttatgcttg atgagtcgtt
      301 ttgagagtaa aaaaatacca gcatatttgc tggtatttga atgaagagag ttagattaga
      361 acgctacgct ttcttttaag ctgacagtta agttaaatac aaggttgtct gccgaaccat
      421 ctttgctgtc taagcagtag taaccttcac gctcgaattg gtagtcttgt tccggtttgg
      481 cgtgagctaa acttgactca acaaaaccat gcttaaccac taatgattca ggatttagca
      541 ccgcattgat gtcttcttcc gcccccgggt ttggtactgt aaataggcgg tcgtaaatgc
      601 ggaactctgc cggtttgtta tcttcagcag atacccaatg aatcacgcct tttactttgc
      661 gaccatcagc cggatttttg cccaatgtat ccgggtcgta tgtgcaaaaa atagttgaaa
      721 ttcgaccgct tgcatctttt tccacacgct cagctttaat cacataggca ttgcgtaaac
      781 gaacttcttt gcctaagact aaacgtttat attgcttatt ggcttcttca cggaagtcgg
      841 cttcatcaat ataaatctca cgggtgaacg gtaaatcacg ctcgccaagc tctggacgat
      901 ttgggtgatt aggggctttt aatatttcct tttcaccgaa gttttcaatc accactcgaa
      961 ccgggttaat caccgccatc gcgcgtggtg cgttttcatt taagtcttca cgaatgcacg
     1021 cttcaagggc agagtattcc accacgttat cttgcttggt taccccaata cggcgacaaa
     1081 attcacgtaa agacgctggg gtataaccac ggcggcgtaa gccggaaatg gttggcatac
     1141 gagggtcatt ccaaccatca acaatgccgt ctgtgactaa ttttaataat ttacgcttag
     1201 aggttaaagt accttctaaa tttaaacgtg aaaattcgta ttggtgcggt aacggtcttt
     1261 caatcgaaat attgtccagc acccaatcat ataagcggcg gttatcttgg aactctagcg
     1321 tacatagcga gtgagtgatt cgctcaatcg catcggaaat gcagtgggta aaatcgtaca
     1381 tcgggtagat gcaccattta tcaccggttt gatgatggtg agcaaatttc acacggtaaa
     1441 gcaccggatc tcgcatcaca ataaacgggg acgccatatc aatttttgct ctcaagcagg
     1501 ctttcccttc cgcaaactct ccgtttttca ttttttcgaa taacgctaag ttttcttcca
     1561 cgctacgatc acggtaaggg ctgtttttgc ccggctcggt taaagtacca cggtattcac
     1621 gcatttcatc aggcgataat tcatcaacat aggctaatcc tttgttgatt aactcaatcg
     1681 catagccata aagctggtca aaatagtctg aggcataacg aggctcaccc tcccatttaa
     1741 aacctagcca ctccacatct tgcttaattg aatctacata ttccacgtct tctttaaccg
     1801 ggttggtatc atcaaaacgc aagttacata aaccgttata atcttgagcg ataccaaaat
     1861 ttaaacagat agatttagcg tgtccgatat gcaaataacc gtttggttca ggtggaaaac
     1921 gagtgtaaac attgttgtgc tttccgcttg ctaaatcttc atcaataata tgggtaataa
     1981 aattggctcg agtgccagtt tctgccgtta aaatttcttc gctcatagtt ctctctaaat
     2041 gtatcaataa aaataacgga ttattctaca cttaattgaa acagacttaa agactaaagc
     2101 aaaattcctc ggtttcctcc aaagaatttt gctgttttag taagatgcaa tggaattgca
     2161 aatttttagc taaatttaac cgcttgttat tgcctgattt cacggctttc gctgatcata
     2221 aaattcgcaa tccgtttggc aatccgccat ttaccctctt gtttaacata ggtatcggca
     2281 taacgcacgc tgtgatcgtg caagatctct ttgccctctt tggtttctac cagtttcacc
     2341 gcacaatagt tgatcgctgt ggcgttattt tcatcaataa aaggcacggt atgctgaccg
     2401 tttaaatgat aaacgctgtg aaacggggtt aaaaaggtgg taaacacctg ttcgatttct
     2461 gccacgccgt gcatttcaaa caccaatttt ccgccgatgt aggtgtttac gacggcatct
     2521 tgcgtgaaaa gcggcatctg ctcggcaacc tttttttcat cggcaaggtt agagaatgta
     2581 tctactaaat cttttagtgc taaacgatct tggatttgtt gtaagttcat tgtgttttcc
     2641 ttctgatttt tgcaaatttt ctttggtttt tcaccgcttg ttaggctgca ataccaacac
     2701 tttcacggat ttcgctgttt tcacgcaaaa tgcgagccac aaaatcggca atggatttgc
     2761 gggaaacgct gtgtcctttg aacggttcgc ctttgtgagt cagctgatat tgcacttccg
     2821 aaccgttggt aaaccagccc gggcggacaa cggtataatc caactcgctg gcttcaatca
     2881 cggctgccga ttggcggtaa ggctctaaaa ttgccccgtg atcttcgccg gtttcgccat
     2941 aaatccccat tgagctaatc caaaccaagc gttttacctg agcttcattc attgcgttca
     3001 ccacatttcc cgccatcgct tttaagtcgc cggcaagtcc agcgtaaacg gcatcaacgc
     3061 cttgcattgc ttgggttaat gcgtggaaat ccaacacatc gccttgcaca attttgacgc
     3121 gttcagtttc aaatttcgct actgattgcg gacgacgggc aaaaagggtt aattgcacat
     3181 tattgtcttt tagcagttca ggaatcacga cattcgctag gctaccgctt gctcctaaaa
     3241 ttaagatctg tttcattgct gttctccatt gaatgaagtc agtatagcgg tgtagattgc
     3301 atttgattag tagggtaaaa tcgcttgaag taatgagaaa aattcattaa taaggtggtt
     3361 aaatgaaaga aaacctcaac gatttaagag cttttttgtt ggtagcacaa acgagcagtt
     3421 ttaccaaagc agcagcacag atgggcgtgt cgcagtcggc gttgtcgcac agcattcggg
     3481 ggattgaaga acggttgcaa atcaagctgt ttcatcgcac cactcgtagc atttcaacca
     3541 ccgaggcagg ggagaggctg tatcaacggc tgttaccgct gtttgatgaa atcgaccaag
     3601 aagtaaatgg gctgagtgag tttcgcaatg cgttaaaagg cgggctgaga attaacggta
     3661 atgaacacgt tttccgtgat gtgctggcag ataaattcgc tgagtttgtc aaacgttacc
     3721 ctgaagtagc attggagcta gtggcggaag atcgctttgt agatattgtc gccgagcgtt
     3781 ttgatgccgg tattcggctt ggggcagatg tggcgaaaga tatgattgca ctacgcattt
     3841 cgcccgatgt gaaaatgacc gcagctgcca gccccaacta tttggcacag cacggcacgc
     3901 cgaaaacgcc ctttgattta accgaacatc attgtctcaa acatcgtctt gcgacattgg
     3961 gcggcattct ggcgtgggaa tttcgggatc ccatcaccaa gaaaatcgtt aaatttcaac
     4021 cgcagggcaa tcttactgcc aataacggct tcttgctgca acactatgcc aaacaaggtt
     4081 taggcattct ttggagtccg caagacagca ttcaaaccga gctggataac ggcagcctcg
     4141 tgccgatttt aaccgactgg gcaatgcagt atgagggcta tcatctctat taccccaacc
     4201 gtcgccaaaa ttcgcctttg ctgaaagcgt taattgatgt gctgagagga taggtaaaaa
     4261 tgagcggtcg tttttagcaa aaaattgacc aaaaaacgac cacttgtaga gaagctaaat
     4321 tatttcacac gagaaacata ttcgcctgaa cgggtatcca cacggataac ttcaccgatt
     4381 tgaacgaata aaggcacgtt taccacagca ccggtgctta atgttgccgg tttaccgcct
     4441 gtacctgcag tatcgccttt taagcccggg tcggtttcga cgatttctaa ctcaacgaag
     4501 tttggtggtg tgactgcaat tgctgaaccg ttccataaag tcacgataca ttctgcatta
     4561 tcaactaacc attttacgtt gtcgcctaac gctttttcat ctaccgagat ttgttcaaag
     4621 gtttcagggt gcataaagta ccagaaatca ccatcgctgt aagagtagtt gtagttagaa
     4681 tctactacat ctgctgcttc aaccgaagtg cctgatttga agttgatctc taatactttg
     4741 ccagagatta atttacggat tttagtacgg gtaaatgctt gacctttacc cggttttacg
     4801 aattcgtttt caacaattac acaaggttcg ccatcttgaa taaattttaa acctttttta
     4861 aagtcgttag tgctgtagct agccattatt tcctcaatta tttaaaaaat aacaataaat
     4921 taacaaattt tgaacccaac cctattgggt ttgtcaaaaa gagccatata ataacctaaa
     4981 ttcacaaaat tagcgaaaga tctcttgcga tttttgctat aatttttcaa actttattga
     5041 taaggaatgc gaatgaaaaa actgattact ctttctgcga ttttagccgt ttctgttgca
     5101 accgtggcag aggcaaaacg tggcggtggg agcttccgtt cagttagccc gtcaaaagcg
     5161 gtagcaacta agccggtagc ttctcaaaaa tcaaccacaa atcagcaaac gcaacaaaaa
     5221 gatgctactt ttgccaatac gccaaacagt gcaactgccc agccacaagt gcgtcaaggc
     5281 agcacgatgg caaatgtagc gatgggggca gcagccggtt atattttagg tgatatgatg
     5341 tcatcaaatg cacatgcaaa tcagcctagc cagcctgcgg atgcaaatgc acaagcggtc
     5401 aattctgcac aaaatgttgc aaatggagca gttcctgcta ttgcagaatt taagagtatt
     5461 ggcggtcaaa tcgatccgtt tttagtagaa aaaaccgatg gttatcgccg ttattgtatt
     5521 tcaggcgtgc aatacctagt cgctgcacaa ggtgtacaaa cctcaccaac tgtaatggtt
     5581 aatccaaatg gcacgccatt acaatgtaac ttaattccat aattattttt atttgcgggc
     5641 aggcaaaaac ctgccccttg tatctacaat attcaatgca accaattcaa atcaaaccgc
     5701 tttggctaac cgagctggct caagccttta ataaccctat cgacttatta caatttcttg
     5761 aactcaatcc aaatgatttt gagggcgata tagctgcccg taaattattt gctcttcgag
     5821 tgccaagaat gttcgccgaa aaaatggaaa agggtaaccc aaacgatccg ctttttttgc
     5881 aagcaatgtc tttacaggcg gaatttattg aggcagaagg gtttgtggta gatccattgg
     5941 aagagcaaca aagcccagca ccgaatattt tacataaata ccataaccgt ttgttattta
     6001 tgctaaaaaa cagttgtgcg attaactgcc gttactgttt tcgccgtcat ttcccttatg
     6061 aggaagtgaa aagcggtaaa gctgtgtggc aacagggact aacttatatt gctgagcatc
     6121 ccgagctgga ggaagtgatt ctgtccggtg gtgatccgct gatggcaaaa gatcaggact
     6181 tagattggat attaacgcaa ttagagcaga tttcgcatat caaaaccctg cgtattcatt
     6241 cacgcttgcc ggtcgttatt cccaatcgaa ttacgacaga attatgtgaa cggttatcaa
     6301 aatctcgctt gaatgtcgtg ttggttaccc atattaatca cgccaatgaa attgatgctg
     6361 tttttgcaaa caaaatggct caactgaaaa aagccggcgt tgtattgctg aatcaatcgg
     6421 tgatgttaaa aggtgtaaac gataatgctc aaaccttaaa aaggttaagt gataaacttt
     6481 ttgaatatgg gattttgcct tactatttgc atttatttga taaagtggca ggggcgagcc
     6541 atttctatat tgaagatgaa caggctggtg agatttaccg agaattacag cgtatcactt
     6601 caggctatct tgtgccaaag ctggcaagag aaatagcgaa agagccaaat aaaattttga
     6661 aaggttagct taaacagaaa tcaagaaaca aaaggcgaaa atggtaaaat tttcgccttt
     6721 tttaaccgct tgttgcaagc ggttaaattc tcttactttt ttgcaaatta gaagttcgca
     6781 ttgcgtggag cacgtgggaa tgggattaca tcacgaatgt tttgtaaccc ggttacatac
     6841 acgattaaac gctcgaaacc taaaccgaaa cctgagtgtg gcactgtacc gtatttacgc
     6901 aaatcacgat accaccagta atcttcaggg tttaagccca tttcattcat acgctgatct
     6961 aacacttcta aacgttcttc acgttgagaa ccaccgataa tttcaccgat tcccggagct
     7021 aaaacgtcca ttgcggcaac cgttttgccg tcatcattta aacgcatata gaaggcttta
     7081 atgtcttttg gatagttttt cacgaccacc ggcgatttaa agtgttcttc ggctaagaaa
     7141 cgctcgtgtt cagaggaaag atcgattccc caagacaccg ggaattcaaa ttctttgccg
     7201 gatttcaata agatctcaat cgcatcggtg taatctactt gagcaaatgg cgaattgata
     7261 aagttttcta aacgactaat cgcttcttta tcaacgtgtt taacgaaaaa ttccatatca
     7321 tctttacgtt ctgcaagcac cgctttaaac acatatttca gcatatcttc ggctaatttt
     7381 gcattgtcgt ttaagtcggc aaaggcaact tccggctcca tcatccagaa ttctgcaaga
     7441 tggcgagttg tgtttgagtt ttctgcacgg aatgtcggac cgaaagtata aactttactt
     7501 aaggcacacg cataggtttc cgcatttaac tggccggata cggttaagaa cgattcttta
     7561 ccgaagaaat cttggttaaa atccactttg ccgttttcgc cacgaggtag attttctaaa
     7621 tccagcgtag aaacacggaa catttcacct gcaccttcag tatctgaggc ggtaattaca
     7681 ggtgttgcta cccaatagaa accttgctcg tggaagaaac ggtggatagc ttgagctaaa
     7741 cagtgacgaa cacgagcaac tgcaccgatt aagttcgtac gagggcgtaa gtgagcgact
     7801 tcacgcagat attcgataga gtggcgtttc gctgccattg ggtaggtatc aggatcttcc
     7861 acccaaccaa gcacttccac tttacttgct tgtaattcaa cggcttgacc ttcagcaggg
     7921 gattccacaa ctttaccggt caccaccaca gagcaacctg cggttaaatg taatacttcg
     7981 gcattataat ttggtagatc gttattaata atcgcttgaa ttgggtcaaa gcaagaacca
     8041 tcgtaaacgc tcaagaaaga taaaccggct tttgaatcac ggcgggtacg cacccaacca
     8101 cgcactgtcg cttcttcacc taccgctatc ttgccggata ggatttccga aactgttgga
     8161 aatttagaca ttgaatacct caaattcttt gttgtgagtt ttaatataag aaacgttccg
     8221 tattctacaa gaaaataggg ggaaatataa taattttacg gaattatttg caaatttgtg
     8281 agtctatata agcattgcaa gtagttaaat ctgattttgc ttggtgttaa aaaatttttt
     8341 attttttaa
//
LOCUS       DANLUQ010000113         8311 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.114, whole genome shotgun sequence.
ACCESSION   DANLUQ010000113 DANLUQ010000000
VERSION     DANLUQ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8311
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.114"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..547)
                     /locus_tag="PXB27_001889"
     CDS             complement(<1..547)
                     /locus_tag="PXB27_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein Hlp"
                     /protein_id="HDL5346648.1"
                     /translation="MNTLAKISLSVLFTLFLTACEQPNSTKTQSSAESPVQVKEESKE
                     EVKPADTGAQDYKMLREWQDTQEKALNDAIKAATETLTDKQKADSTLMQETVNNALLA
                     QIDHIKISAETLNIQNNEVKALKDKTLEVLTLGAQMIVEGAKMEKNPTPEAHKAFGEL
                     QTKLNQLAEEGQQLENILRAKY"
     gene            complement(614..1198)
                     /locus_tag="PXB27_001890"
     CDS             complement(614..1198)
                     /locus_tag="PXB27_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein HlpB"
                     /protein_id="HDL5346649.1"
                     /translation="MNKLTKISATALVALFLAACDKPAEKAPATEPTKTTEAAPTTTA
                     PAAEAAKPAETAVAAPSEAAKADYQKLVDWNTAQESTMATAQAELQKKLATQDPKQIQ
                     EGLSAFNKKVEETVKSLDAIEVSDAQIKTFKEKTKSVLTLSSEVISEQVKTISTPNDQ
                     AALQAVQQKAQSLIEAGNELQKLNVELQQRFSAK"
     gene            complement(1330..2514)
                     /locus_tag="PXB27_001891"
     CDS             complement(1330..2514)
                     /locus_tag="PXB27_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HDL5346650.1"
                     /translation="MKSADIVIVGGGMVGLALAGLLKDVDCQIKIIEKNTPKFDADTI
                     YHRVSAINASSQKILEQIGAFQHIPAQRLSPYTEMKVWEKDSFANIHFDNNDNEIKQL
                     GLSQLGFILENAVIQHALWQQVSQQANVEYIIAAPQTLGVSENGAFLTLDNGEMLSAK
                     LVVGADGANSWVRNQMKMPLTSKDYQHTALVCNVKTTEKHGKTARQIFAEDSIFAFLP
                     MADEHLCSIVWSLPPNKANELKNASEAEFNKALTIAFDNQLGLCEVQSLREIYPLAAR
                     FCRDFAQPRIALIGDAAHTIHPLAGLGVNLGFADARTLANEIQKQLVNNQDIGEYRHL
                     RQFERERKAQAMKLLATMEGLKQLFDGNHPLKKLFRGIGLSLTNQLPFVKKQLIQQAI
                     YL"
     gene            complement(2597..3460)
                     /gene="rapZ"
                     /locus_tag="PXB27_001892"
     CDS             complement(2597..3460)
                     /gene="rapZ"
                     /locus_tag="PXB27_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDL5346651.1"
                     /translation="MELIIISGRSGSGKSVALRALEDAGYYCVDNIPLPLIPTLTYYL
                     TQENRSAVISLDIRNLPNTPNAIEELLTQLEPLNAKLIFLDCERNTLIRRYSDSRRIH
                     PLSTQDDLSLEGALDLEQKLLEPLVQNANYIIDTSHLSSHELAENLRQILQGSTEKEL
                     KIIFQSFGFKYGLPADSDYVFDVRFLPNPHWNPELRPMTGLEQPVIDFLERQTEVHNF
                     IYQTRNYLEMWLPMLEQNNRSYLTIAIGCTGGKHRSIFIAEQLAKYFQAKGKNVQVRH
                     KSLEKHHKKTS"
     gene            complement(3473..3985)
                     /locus_tag="PXB27_001893"
     CDS             complement(3473..3985)
                     /locus_tag="PXB27_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL5346652.1"
                     /translation="MTKLTEFLSPENVRQGVLISSKKRALELVGKIVAESINQTEQQS
                     EALCPIECFGNLFKREKLGTTGLNQGIALPHAKLPACGDIELTKPIAVFLQLEEAIDY
                     EAQDNKEVDLIYAMMFPENSCEQYKSCLPAIAQKLTDKSLLKQLRAAESAEDIWQILV
                     YADNHLKDEE"
     gene            complement(4027..4752)
                     /gene="lptB"
                     /locus_tag="PXB27_001894"
     CDS             complement(4027..4752)
                     /gene="lptB"
                     /locus_tag="PXB27_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005727816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HDL5346653.1"
                     /translation="MSVLYAEHLAKSYKQRQVVKDVSLNVKSGEIVGLLGPNGAGKTT
                     TFYMVVGLVRHDHGTIRIDDQDISTLPMHDRAKQGIGYLPQEASIFRRLSVYDNLMSV
                     LQVRKNINDKQRKERAEELITEFHIEHIRNSLGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKKIIVNLKERGLGVLITDHNVRETLDVCERAYIVGSGEMIAS
                     GTPQQVLENPDVKRVYLGDQFKL"
     gene            complement(4764..5270)
                     /gene="lptA"
                     /locus_tag="PXB27_001895"
     CDS             complement(4764..5270)
                     /gene="lptA"
                     /locus_tag="PXB27_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HDL5346654.1"
                     /translation="MKLMFKSILVSVLLGSSFSAYALKGDTDQPINIDSGSQSLDMTN
                     NIVTFSDNVVITQGSIKVTAANVKITRQEGKKETIDATGSPVTFQQTLDNGKPVNGKG
                     NSVHYDLNSEFLTLIGNAELKQQGSFIKANKITYDVKKQQLKATSGGKTRVKTVLIPN
                     ELQDNKKK"
     gene            complement(5294..5878)
                     /gene="lptC"
                     /locus_tag="PXB27_001896"
     CDS             complement(5294..5878)
                     /gene="lptC"
                     /locus_tag="PXB27_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HDL5346655.1"
                     /translation="MNKRLTVILLIIVAVLGGWYYSLQEKENTGLDQLIKKEGQPEYV
                     GNKMSTTVYDLKGNPQYFAQATEIKRYESTERTEFFKPFIELFAKESTQKQWKVSADY
                     AEITKEKMLNLKGNIKLVALDPASRLQQITTDTLTVDLNTQDIFTESTVKSIGSGFTT
                     TGVGLKGNLKQQVATLQKDVKSYIEPTIIQETKE"
     gene            complement(6032..6835)
                     /gene="xthA"
                     /locus_tag="PXB27_001897"
     CDS             complement(6032..6835)
                     /gene="xthA"
                     /locus_tag="PXB27_001897"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDL5346656.1"
                     /translation="MKFISFNINGLRARPHQLEAIIEKHQPDVLGLQEIKVADEDFPY
                     DLVNHLGYHVFHHGQKGHYGVALLTKKEPIAVRKGFPTDVLDAQKRMIMADIETDFGV
                     LTVLNGYFPQGENRSHETKFPAKQKFYADLQNYLESELKPENPIIIMGDMNISPTDLD
                     IGIGEPNRKRWLRDGKCSFLPEEREWLDRLYSYGLVDTFRAGNPDVNDKFSWFDYRSK
                     GFDDNRGLRIDLILASKSLAERCVETGIDLDIRAMEKPSDHSPVWATFK"
     gene            complement(6933..8309)
                     /locus_tag="PXB27_001898"
     CDS             complement(6933..8309)
                     /locus_tag="PXB27_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005617456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HDL5346657.1"
                     /translation="MLSNEVFISIIVLLILSLLRINVVIALIISALTCGLIGNLGVEG
                     AGFTEALEKTIKSFTGGLGGGAETAMNYAVLGAFAVALSKSGVTDLLAYKVIKSFGHS
                     PSGRSVTWFKYIVLFVLVAFAMSSQNLIPIHIAFIPILIPPLLSVMNKLQIDRRAVAC
                     ALTFGLTATYMLIPAGFGQIFIESILVKNINQAGEPFQLVTTTAEMTKAMLIPVSGMI
                     LGLLFAFFVSYRKPRVYIENMPEPTADDIEARVANVKPFHIGISIVAIIATCSVQLAT
                     NSTIIGGLVGLVIFALGGVFRLKESNDVFQAGLRLMAMIGFVMIAASGFAGVVNSTGG
                     VPALVESLRDVISSKEMAALLMLVVGLFITMGIGSSFSTVPIITSIYVPLCISFGFSP
                     LATMAIVGVAAALGDAGSPASDSTLGPTSGLNADGKHDHIWDSVVPTFIHFNIPLLVF
                     GWIAAMTL"
BASE COUNT         2310 a         1662 c         1676 g         2663 t
ORIGIN      
        1 catatttagc tcttaatata ttttctaact gttgcccttc ttcagctaat tgattcaatt
       61 ttgtttgtaa ctcgccaaat gctttatgtg cttccggtgt aggatttttc tccattttag
      121 caccttctac gatcatctga gcccctaaag tcaggacttc taaggttttg tcttttagtg
      181 ctttgacttc attattttga atattcaggg tttcagcact tatcttaata tgatcaattt
      241 gagctaataa tgcgttatta actgtctctt gcattaaggt agaatcggct ttttgtttgt
      301 cagtaagtgt ttctgttgcc gctttaattg catcatttaa ggctttttct tgagtgtctt
      361 gccattcccg taacatttta taatcttgag cgccggtatc agcaggttta acttcttctt
      421 ttgactcttc tttcacttga actggcgatt ctgctgagct ctgtgttttg gtagaattag
      481 gttgttcgca agcggttaaa aaaagggtaa ataatacaga taggctaatt ttagcgagcg
      541 tgttcatagt aatccttagt aattgcaaaa aaaagctaga gcaaaatact ctagcttttc
      601 attgaatggt aaattattta gctgagaatc tttgctgtaa ttctacgttt aatttttgta
      661 actcattacc agcttcaatt aaagactgag ctttttgttg tacggcttgt aatgctgctt
      721 ggtcatttgg tgtagagatc gtttttactt gttcagaaat cacttcactt gaaagtgtta
      781 aaactgattt tgttttttct ttaaatgttt tgatttgtgc atcagatact tcaatcgcat
      841 ctaagctttt tacagtttct tctacttttt tattaaatgc acttaatcct tcttgaattt
      901 gtttaggatc ttgtgtagct aatttctttt gtagttctgc ttgtgctgtt gccattgtgc
      961 tttcttgtgc cgtattccaa tcaactaatt tttggtaatc tgcttttgca gcttcgcttg
     1021 gagcggctac agctgtttca gcaggtttgg ctgcttctgc agcaggagct gttgtagttg
     1081 gtgcagcttc agttgttttt gtaggttctg ttgccggtgc tttttctgct ggtttatcac
     1141 aagctgctaa gaataatgcg actaaagcgg ttgcactgat ttttgttaat ttgttcatta
     1201 aaaaattcct caagaatata aaaataaaag ccaataaagg ctggttattg taccaataaa
     1261 tagacaagta atctccgtat tagttcataa aatcgttcat ctttacgtta tttcgaaaaa
     1321 atttacattt tataagtaaa tagcttgctg aataagctgc tttttcacaa aaggcagctg
     1381 gttggttagg cttaaaccaa ttcctctgaa taatttttta agcgggtgat tgccgtcaaa
     1441 tagctgtttt aatccttcca ttgtggctaa taatttcatc gcttgggctt tacgctcacg
     1501 ttcaaattgg cgtaaatggc ggtattcacc aatatcttgg ttgtttacca actgtttttg
     1561 gatttcgttt gccagcgttc tggcatcggc aaagcctaaa ttgacaccaa gtcccgccaa
     1621 tgggtgaatg gtatgagcgg catcaccaat caacgcaata cgaggttggg caaagtcacg
     1681 gcagaagcgt gctgcaagcg ggtaaatttc acgcaaactt tgcacttcac ataagccaag
     1741 ctgattatcg aaggcaatcg ttaaggcttt attaaattcg gcttcgcttg cattttttaa
     1801 ttcattggct ttgtttggtg gcagcgacca aacaatcgag cagagatgct catctgccat
     1861 tggcagaaaa gcaaaaatgc tatcttcagc aaagatttga cgagcggttt tgccgtgctt
     1921 ttcggttgtt ttgacattac agactaaggc ggtatgttga taatctttac tggtaagcgg
     1981 cattttcatc tggttacgca cccacgaatt tgcaccgtct gccccgacaa ccaatttagc
     2041 agacagcatt tcgccgttat ctaaggttaa aaatgccccg ttttcgctca cgcccagagt
     2101 ttggggggcg gcgataatat attctacatt agcttgttga gaaacctgtt gccacaatgc
     2161 gtgttgaatc accgcatttt ccaaaataaa gccaagttgt gaaagtccta attgcttgat
     2221 ttcattatca ttattatcaa aatggatatt agcaaagctg tctttttccc acactttcat
     2281 ttcggtgtaa ggagaaaggc gttgagccgg aatgtgttga aaagcaccaa tctgttccag
     2341 tattttttgg cttgaagcat taatcgcact tacacggtgg taaatcgtat cggcatcaaa
     2401 tttgggcgtg tttttctcaa taattttaat ttggcaatcg acatctttga gcagtccggc
     2461 aagagccagc ccaaccatac cgccgccaac aataacaata tctgcggatt tcataaaatc
     2521 aaatgtaggg gcggtgccct gtgcccgccc taaaatagtt gaaatgaggg ctggcacagg
     2581 accagcccct acttatttaa gatgtttttt tatggtgttt ttccaaactt ttatgccgaa
     2641 cttgcacatt tttgcctttt gcttggaaat attttgccag ttgctcggca ataaatatgg
     2701 aacgatgttt tccaccagtg caaccaatcg caatagttag gtagctacgg ttattttgtt
     2761 ccaacatcgg cagccacatt tctaaataat tgcgggtttg gtaaataaaa ttatgcactt
     2821 cggtttggcg ttctaagaaa tcaatcaccg gttgctctaa gcctgtcatt gggcgaagct
     2881 ccggattcca atgtgggttc ggtaaaaagc gaacatcaaa aacgtaatcg gaatcggcag
     2941 gtaagccgta tttaaaaccg aatgattgga aaataatttt aagctctttt tcggttgagc
     3001 cttgcagtat ttggcgaaga ttttccgcta attcgtgcga agaaagatga ctggtgtcaa
     3061 taatataatt tgcattttgc accaaaggct caagcaattt ttgttccaaa tccaatgcac
     3121 cttctaacga gagatcgtct tgggtggaaa gcgggtggat tcggcgggaa tcgctataac
     3181 ggcggattaa ggtattgcgt tcgcaatcta aaaaaatcag cttagcattt aaaggctcaa
     3241 gctgggtaag caattcttca atcgcattag gcgtattcgg taaattacga atatctaagc
     3301 tgatgactgc cgaacgattt tcttgagtaa ggtagtaggt taaggttgga attaaaggta
     3361 aagggatatt atccacgcag taataacctg catcttctaa tgctcttaat gctaccgatt
     3421 taccagagcc tgagcgtccg cttataatga ttaattccat atttcctcag tattattctt
     3481 catcttttag gtgattatca gcatacacca aaatttgcca aatatcttct gcggattcag
     3541 ctgctcggag ctgttttaat aacgatttat cggtaagttt ctgtgcaatt gcaggtaggc
     3601 aacttttata ctgttcgcag ctattttccg gaaacatcat tgcataaatt aaatccactt
     3661 ctttgttgtc ttgggcttcg tagtcaatcg cttcttccag ttgtaaaaag accgcaatcg
     3721 gtttagtcag ctcaatatca ccgcaagcgg gcaattttgc atgaggaagt gcaatgcctt
     3781 gatttaagcc ggttgtgcct aacttttcac gtttaaataa attgccaaag cattcaatag
     3841 ggcagagtgc ttcagattgc tgttcggttt ggttgattga ttcagccacg attttaccca
     3901 cgagttctaa cgctcgtttt ttacttgaaa tcagtacacc ttggcgaaca ttttcagggc
     3961 ttagaaattc ggttagtttt gtcataattt ttcatctaaa gatgaaatcc ccaaagaggg
     4021 gattttttag agtttgaatt gatcgcctaa ataaacacgt tttacatcag ggttttcaag
     4081 cacttgctgt ggtgtgccgc tggcaatcat ttcgccgcta ccgacaatgt aggctctttc
     4141 acatacatct agagtttcac gtacgttatg gtcggtaatc aataccccta aaccacgctc
     4201 ttttaaattg acgataattt ttttaatatc aattaccgag attgggtcaa ccccagcaaa
     4261 aggttcatct aacagaataa atttcgggtt cgctgctaat gctcgggcaa tttccacacg
     4321 gcggcgttca ccacctgaaa gtgattgacc taaactattg cgaatatgct caatatggaa
     4381 ttctgtaatc agctcttcgg cacgctcttt acgctgttta tcattgatat ttttacgcac
     4441 ttgtaatacc gacattaagt tatcgtacac acttaaacgg cggaaaatag atgcttcttg
     4501 cggtagatag ccaatccctt gttttgctct gtcgtgcatt ggtagtgtgc tgatatcttg
     4561 gtcgtcaata cgaattgtgc cgtggtcgtg gcgaaccaaa cccacaacca tatagaaagt
     4621 ggtggtttta cctgcgccgt tcggaccgag caagcccacg atttcacctg atttgacatt
     4681 caggctgacg tcttttacca cttggcgttg tttatagctt tttgctaaat gttcggcata
     4741 aagcacagac atatcattta accttatttt ttcttgttat cttgaagttc atttggaatt
     4801 agcacggttt tcactcgggt tttaccgcca ctggtagctt taagttgttg ttttttcaca
     4861 tcataggtaa ttttattggc tttaataaag ctaccttgtt gtttgagttc cgcattgcca
     4921 atcagcgtta aaaattcaga gtttaaatca taatgaacgc tgttgccttt accgtttacc
     4981 ggtttaccgt tatctaaggt ttgttggaat gttacaggtg agcctgtagc gtcaatagtt
     5041 tcttttttac cttcttgacg ggtaattttg acgtttgctg cggtcacttt aatcgaccct
     5101 tgagtgataa cgacattatc gctaaaggtc acgatattgt tagtcatatc taacgattgg
     5161 ctacctgaat caatattaat cggctgatcg gtatcgcctt ttagagcata agcggaaaag
     5221 ctgctaccca gtaacacgct cactaaaata gatttaaaca ttaatttcat tgttattcct
     5281 tgttccaagc aaattattct ttagtttctt gaataatggt cggttcaatg taagatttta
     5341 catctttttg caaagtcgca acttgttgtt ttaagttacc ttttaatcca acccccgttg
     5401 tagtaaaacc ggaacctatc gatttgaccg tactttcggt aaaaatatcc tgtgtattta
     5461 aatcaacagt taacgtatcg gtagtgattt gttgcaaacg tgatgcagga tctaatgcaa
     5521 caagtttgat attacctttt aaatttaaca ttttttcttt ggtaatttct gcataatcgg
     5581 cactgacttt ccactgtttt tgcgtcgact ctttggcaaa aagctcaata aaaggtttaa
     5641 aaaactcagt acgctcagta gactcatagc gtttaatttc agtagcttga gcgaaatatt
     5701 gtgggtttcc ttttagatca tagaccgtgg tggacatttt attccccaca tattcaggct
     5761 gtccttcctt tttaataagc tgatcaagcc ccgtattctc tttctcttgt aagctgtaat
     5821 accagccacc aagtacggca acaataatca gtaaaataac ggttaaacgt ttgttcataa
     5881 tccaaaaata atctaaaaat agtgtttgat tgtagcacag atttgaatag ggatatagta
     5941 cagcgtaaaa agtgcaaaaa agtgagaaaa aataaccgct tgtagggcta aaaactgaca
     6001 agcggttgaa aactcagatt tttttgcaac tttatttaaa ggttgcccaa acaggggagt
     6061 ggtcagacgg tttttccatt gctcgaatgt ctaaatcaat gcctgtttcc acgcaacgct
     6121 cagcaagtga ttttgaagca agaattaaat caatacgcaa gccacgatta tcatcaaaac
     6181 cttttgaacg gtagtcaaac caagagaact tatcgttcac gtctgggttg cctgcacgga
     6241 aagtatcaac caagccgtag ctgtataagc gatctagcca ttcacgctct tcgggtaaga
     6301 atgaacattt accgtcacgc agccagcgtt tgcggtttgg ctcgccgatg ccgatatcca
     6361 aatcagtcgg gctgatgttc atatcgccca taataataat cggattttcc ggctttaatt
     6421 cgctttccaa gtagttctgc aaatcagcat agaatttttg ttttgccggg aatttggttt
     6481 cgtggctgcg attttccccc tgtgggaaat agccgtttaa tacggttagc acgccgaaat
     6541 cggtctcgat atccgccata atcattcgtt tttgagcatc aagtacatct gtcgggaagc
     6601 ctttacgcac cgctataggc tcttttttag tcagtaatgc cacgccgtaa tgacctttct
     6661 gcccgtggtg gaaaacgtgg tagcctaagt gattgactaa atcgtaagga aaatcttcat
     6721 cggcaacttt gatttcttgt aagccgagta catcgggctg gtgtttttcg ataatcgctt
     6781 cgagctgatg tggtctggct cgtaatccgt tgatattaaa agagataaat ttcattggtg
     6841 atcctaatgt ggtttgcaaa ataaagaaga aaatagaccg cttgtaatgg ctacaagcgg
     6901 tcatttttag caaaaatttt gcatattata gcttaaagtg tcattgctgc aatccagccg
     6961 aacactaata atggaatgtt aaagtggata aaagttggta caacagaatc ccaaatgtgg
     7021 tcgtgtttac cgtcagcatt caagccggag gttggaccaa gtgtggaatc tgatgccgga
     7081 gaacctgcat caccgagtgc agcagcaaca ccgacaatcg ccattgtcgc cagtggcgag
     7141 aagccaaatg agatacaaag cggcacataa attgaggtaa tgatcggcac agtcgagaat
     7201 gaagagccga tacccatcgt aataaataag ccaacgacta acattagcaa tgctgccatt
     7261 tcttttgatg aaatcacatc acgcaggctt tctaccaatg ccggcacacc gcctgttgaa
     7321 ttgaccacgc cggcaaaacc ggaggcggca atcatcacaa agccaatcat cgccattaaa
     7381 cgtaagccag cttggaagac atcgttactc tctttcagtc taaatacacc gcctaaggcg
     7441 aaaataacca gaccgactaa gccgccgata atagtcgaat ttgttgctaa ttgcaccgaa
     7501 caggtcgcga taatcgccac aatactaatg ccgatatgga agggcttaac gtttgcaaca
     7561 cgggcttcaa tatcatctgc ggttggttca ggcatatttt caatatacac acgcggttta
     7621 cggtatgaca caaagaatgc aaacagtaag cctaaaatca taccggaaac aggaattaac
     7681 atcgctttgg tcatttccgc tgtggtagtc actaattgga acggttcacc tgcttggttg
     7741 atatttttaa ctaagatgct ttcaataaag atctgaccga aacctgccgg aattaacata
     7801 taagtggcgg ttaaaccaaa ggttaaagca caagctaccg cacggcggtc aatttgcaat
     7861 ttattcatta cgcttaatag cggtggaatt aaaatcggaa taaaggcaat atgaattgga
     7921 attaggtttt gagatgacat tgcaaatgcc actaacacaa ataatacaat gtatttaaac
     7981 caagtaacgg agcgacctga cgggctgtga ccaaaggatt tgatcacttt ataggctaat
     8041 aaatcggtca cgcctgattt tgatagagct acggcaaacg caccaagaac ggcatagttc
     8101 atagcagttt ccgcaccccc acctaaacca ccagtaaaac tttttattgt tttttccaat
     8161 gcttcggtaa atccagcccc ttctacacca agattaccga ttagaccgca agtaagggca
     8221 gaaataataa gtgcgattac tacattaatt ctaagcaagc ttaggattaa cagcacgata
     8281 atcgaaataa agacttcgtt tgataacatt g
//
LOCUS       DANLUQ010000114         8187 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.115, whole genome shotgun sequence.
ACCESSION   DANLUQ010000114 DANLUQ010000000
VERSION     DANLUQ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8187
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.115"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            34..1026
                     /locus_tag="PXB27_001899"
     CDS             34..1026
                     /locus_tag="PXB27_001899"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015701738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HDL5346658.1"
                     /translation="MKKSFILQQQEISFVKNTFTQYLIDKLGIIEVQGPILSEVGNGM
                     QDNLSGIEKAVKVNVKCIPSATYEVVHSLAKWKRHTLARFGFKEGEGLFVHMKALRPD
                     EDSLDRTHSVYVDQWDWEKVIPEGRRNFDFLKETVNEIYKAIRLTELAVEARFDIPSV
                     LPKQITFVHSEDLVQRYPNMTSKERENAICKECGAVFLIGIGGKLSDGKPHDGRAPDY
                     DDWTTESEQGYKGLNGDILVWNEELGTAFELSSMGIRVDETALRLQVALTGDEDRLEM
                     DWHKDLLAGRLPLSIGGGIGQSRLVMFLLRKKHIGEVQSSVWPKAVFEEFENIL"
     gene            complement(1150..1569)
                     /gene="atpC"
                     /locus_tag="PXB27_001900"
     CDS             complement(1150..1569)
                     /gene="atpC"
                     /locus_tag="PXB27_001900"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDL5346659.1"
                     /translation="MASQFELTVVSAERKIFEGTVTSVRVSGTDGELGVYAGHAPLLT
                     AIKPGMVKFTLADGKEEFIYVSGGFLEVQPTVVTVLADTAIRGEELDEQRILAAKRKV
                     EETLSKTSDADMTAKLAREIAKLRVYELTKTKLANKR"
     gene            complement(1597..2970)
                     /gene="atpD"
                     /locus_tag="PXB27_001901"
     CDS             complement(1597..2970)
                     /gene="atpD"
                     /locus_tag="PXB27_001901"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011978979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDL5346660.1"
                     /translation="MATGKIVQIIGAVIDVEFPQDAVPKVYDALKVESGLTLEVQQQL
                     GGGVVRCIALGSSDGLKRGLKVENTNKAIEVPVGTKTLGRIMNVLGEPIDEAGPIGEE
                     ERWTIHRAAPSYEEQANSTELLETGIKVIDLVAPFAKGGKVGLFGGAGVGKTVNMMEL
                     IRNIAIEHSGYSVFAGVGERTREGNDFYHEMKDSNVLDKVSLVYGQMNEPPGNRLRVA
                     LTGLTMAEKFRDEGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEMGVL
                     QERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDSTVVLSRNIASLGIYPAVDP
                     LDSTSRQLDPLVVGEEHYNVARGVQGTLQRYKELKDIIAILGMDELSEDDKRLVARAR
                     KIERFLSQPFHVAEVFNGVPGKFVPLKETIRGFKGILEGEYDHIPEQAFYMAGSIDEV
                     VERAKQM"
     gene            complement(2991..3857)
                     /gene="atpG"
                     /locus_tag="PXB27_001902"
     CDS             complement(2991..3857)
                     /gene="atpG"
                     /locus_tag="PXB27_001902"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011201492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDL5346661.1"
                     /translation="MAGAKEIRTKIASVRNTQKITKAMEMVAASKMRKTQERMAASRP
                     YAESIRKAISHIAKGNIEYKHPFLTPRPVKKVGYLVVSTDRGLCGGLNINLFKTVLHE
                     LKEKDDQGVKSRLAVVGNKGISFFNPMGLEIKGHINGLGDTPAMEDLVGIVNGMVNAY
                     REGEIDEVYVVYNRFINTMSQKPTVQQLLPLPALENDSLEQTGSWDYLYEPNPQALLD
                     SLLVRYLESQVYQAVVDNLASEQAARMVAMKAATDNAGNLINELQLVYNKARQASITN
                     ELNEIVAGAAAI"
     gene            complement(3883..5424)
                     /gene="atpA"
                     /locus_tag="PXB27_001903"
     CDS             complement(3883..5424)
                     /gene="atpA"
                     /locus_tag="PXB27_001903"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDL5346662.1"
                     /translation="MQLNSTEISELIKKRIAQFNVVSEAQSTGTIVSVSDGVIRIHGL
                     SDVMQGEMIALPGNRYAIALNLERDSVGAVVMGPYADLAEGMQVQCTGRILEVPVGRG
                     LLGRVVNTLGQPIDGKGEIQNDGFSPIEVIAPGVIDRQSVDQPVQTGYKAVDSMVPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTIANVVRKLEEHGAL
                     ANTIVVVASASESAALQYLAPYAGCAMGEYFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERASRVNADYVERFTNGAVKGQTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLESGLFNAGIRPAVNPGISVSRVGGSAQTKLVKKLAGG
                     IRTALAQYRELAAFAQFASDLDEATRKQLSHGQKVTELLKQKQFEPMSVAQLGLSLAA
                     AEYGYLDDIPVERVGSFEANLLAYAQSNYGEFMQELSKSGNFNDEIKDKLNEILSGFK
                     KNSAW"
     gene            complement(5437..5970)
                     /gene="atpH"
                     /locus_tag="PXB27_001904"
     CDS             complement(5437..5970)
                     /gene="atpH"
                     /locus_tag="PXB27_001904"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDL5346663.1"
                     /translation="MSELSTVARPYAKAAFDFALEQGQLEQWQQMLTFSAAVANDEQV
                     ASFLNSSLASGQISETFIKICGEQLDQYGQNFIRVMADNKRLAVLPAVLNAFLELRAE
                     QESIKDVEVVSADKLTDAQEAKIANAMEKRLGTKVRITSSIDSSLIAGVIIRYDDVVI
                     DGSSRGQLNRLSQELSL"
     gene            complement(5984..6454)
                     /gene="atpF"
                     /locus_tag="PXB27_001905"
     CDS             complement(5984..6454)
                     /gene="atpF"
                     /locus_tag="PXB27_001905"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDL5346664.1"
                     /translation="MNLNATLIGQLIAFALFVAFCMKFVWPPLIRVIEERQANIASAL
                     ASAEKAKQEQADSKAAADQEILRAKEEAQNIIDLATKRRNEILESVQAEAEVERQRII
                     EQGYAEVESERKRVQEELRQKVAALAIAGAEKIVGRSVDAAANNDIIDKLVAEL"
     gene            complement(6515..6769)
                     /gene="atpE"
                     /locus_tag="PXB27_001906"
     CDS             complement(6515..6769)
                     /gene="atpE"
                     /locus_tag="PXB27_001906"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HDL5346665.1"
                     /translation="MESVITATIIGASILLAFAALGTAIGFAILGGKFLESSARQPEL
                     ASSLQTKMFIVAGLLDAIAMIAVGISLLFIFANPFIDLLK"
     gene            complement(6887..7675)
                     /gene="atpB"
                     /locus_tag="PXB27_001907"
     CDS             complement(6887..7675)
                     /gene="atpB"
                     /locus_tag="PXB27_001907"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005713638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDL5346666.1"
                     /translation="MAGTTAEYISHHLSFLSTGEGFWAVHLDTLFFSLVAGAIFLWVF
                     SRVAKNATTGVPGKLQCFVEIVIEWVNGLVKDNFHGPRNVIAPLALTIFCWVFIMNAI
                     DLIPVDFLPQLANLFGIHYLRAVPTADISATLGMSICVFALILFYTVKSKGFGGLVKE
                     YTLHPFNHWAFIPVNFILETVTLLAKPISLAFRLFGNMYAGELIFILIAVMYMADNFA
                     LQALGIPLHLVWAIFHILVITLQAFIFMMLTIVYLSIAYNKADH"
     gene            complement(7698..8081)
                     /locus_tag="PXB27_001908"
     CDS             complement(7698..8081)
                     /locus_tag="PXB27_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HDL5346667.1"
                     /translation="MSAVINRAKSQYKQALKVEFFLLCVFALGVSLWKMNFNFSFLGG
                     ALASFLPHLVFVYWVFFKKSAKNQSKMTAFYRGEGMKWLVTIILVIGCFKLIPALDVV
                     LFFVGYFIALFLNNMIPFVLSKHHH"
BASE COUNT         2432 a         1842 c         1531 g         2382 t
ORIGIN      
        1 aaaatcaaca ttctttaatt aggaaagcaa acaatgaaaa aatcttttat tttacagcaa
       61 caagaaatca gttttgttaa gaacacattt actcaatatt taattgataa attaggcatt
      121 attgaagttc aggggcctat tttgagcgaa gtcggcaacg gtatgcaaga caacctttcc
      181 ggtatcgaaa aagcagtaaa agtaaacgta aaatgtattc caagcgctac ttatgaagtg
      241 gttcattctc ttgcaaaatg gaaacgccac accttagctc gttttggatt taaagaagga
      301 gaagggttat ttgttcatat gaaagctctt cgccctgatg aagattcttt agatcgcaca
      361 cactcggtat atgttgacca atgggactgg gaaaaagtca ttcctgaagg tcgccgcaac
      421 ttcgatttct taaaagaaac ggtaaacgaa atctataaag caattcgttt aaccgaatta
      481 gcggtggagg ctcgtttcga tattccatct gtattgccta aacaaattac tttcgttcac
      541 agcgaagact tagtgcaacg ctatccgaat atgaccagca aagagcgtga aaatgctatc
      601 tgtaaagagt gcggagcggt gttcttaatc ggtatcggcg gcaaattatc agacggtaaa
      661 cctcacgatg gacgagctcc ggactatgat gactggacaa ccgagtccga acaaggctac
      721 aaaggcttaa acggcgatat tttagtttgg aacgaagaac tcggcacagc ctttgaactt
      781 tcctcaatgg gtattcgtgt agatgaaacc gccttacgct tacaagttgc tttaactggc
      841 gacgaagacc gcctcgaaat ggattggcac aaagatttat tagccggcag actcccgctt
      901 tctatcggcg gcggtatcgg tcaatctcgc ttagtgatgt tcttactacg caaaaaacac
      961 atcggtgagg tgcaatccag tgtctggcca aaagcggtat ttgaagagtt tgagaatatt
     1021 ctttaataaa acaaaaaagc gaaccttagg gttcgctttt gctttctaca agcggtctta
     1081 ttttacaaaa aatttactgt ttttaaccgc ttgtaaaaca aaaccccact ttcgtggggt
     1141 tttttgatat taacgtttat tcgctaattt ggttttagtg agttcgtaaa cacgcagttt
     1201 cgcaatttct ctcgctaact tcgcggtcat atccgcatcg ctggtttttg aaagtgtttc
     1261 ttctactttg cgttttgcgg caagaatacg ctgttcgtct agttcttcac cacgaattgc
     1321 ggtatcagcc aacactgtta ctacggtcgg ttgtacttca aggaaaccac ctgaaacgta
     1381 gataaactct tctttgccgt cagctaaagt gaatttaacc attcccggtt taattgcggt
     1441 taataacggt gcatgccctg catacacccc taattcacca tctgtccctg aaacacgcac
     1501 gctggtaact gtgccttcaa agattttacg ttctgcactc actaccgtga gttcaaattg
     1561 agatgccatg ttgttctcct ttagtttaga acaaaattac atctgttttg ctctttcaac
     1621 cacttcgtca attgaacccg ccatatagaa cgcttgttcc ggaatatggt cgtattcgcc
     1681 ttctaaaatg cctttaaagc cacgaattgt ttcttttaac ggtacgaatt tgcccggtac
     1741 gccgttgaat acttccgcaa cgtggaatgg ttgtgaaagg aaacgttcga ttttacgcgc
     1801 acgagcaaca aggcgtttgt cgtcttccga taattcatcc atacccaaaa ttgcaatgat
     1861 gtcttttaac tctttataac gttgtaatgt accttgtaca ccacgagcaa cgttatagtg
     1921 ttcctcacca accacgagtg gatctaactg acgagaggtt gagtcaagag ggtcaaccgc
     1981 cgggtaaata ccgagtgaag cgatattacg gcttaatacc accgttgagt ctaagtgggc
     2041 aaaggtagtt gccggagacg ggtcagttaa gtcatccgcc ggaacatata ccgcttgcac
     2101 agaggtaata gagccggttt tggttgaggt aatacgctcc tgtaatacgc ccatctcttc
     2161 tgcaagagtc ggctgataac ctaccgcaga cggcatacga cctaataacg cagatacttc
     2221 ggtaccagct aaagtataac ggtagatgtt atccacgaag aataatacgt cacgaccttc
     2281 atcacggaat ttttctgcca tggttaagcc ggttaatgcc acacgcagac ggttgcccgg
     2341 tggttcgttc atttgaccgt aaaccagcga tactttgtct aatacgttag agtctttcat
     2401 ttcgtggtag aagtcattac cctcacgagt acgctcacct acaccggcga ataccgagta
     2461 accgctatgt tcgatagcga tgttacggat taactccatc atatttacgg ttttacctac
     2521 acccgcacca ccgaataagc caactttacc acccttagca aatggagcaa ctaagtcgat
     2581 aactttgata ccggtctcta aaagctcggt gctgttcgct tgttcttcgt agcttggtgc
     2641 tgcacggtgg attgtccaac gttcttcttc accgattggg cctgcttcat caattggctc
     2701 acctaatacg ttcataatac gaccgagtgt ttttgtacca accggtactt caatcgcttt
     2761 attcgtattt tcaactttta agccacgttt taaaccatca gatgaaccaa gtgcgataca
     2821 acggaccaca ccgccaccta attgttgttg aacttcaagg gttaatcctg attcaacttt
     2881 taatgcatca taaacttttg gtactgcatc ttgcggaaac tcaacatcga ttaccgcacc
     2941 gatgatctgt acaatttttc ccgttgccat taccgttcct ctattcattg ttaaattgct
     3001 gcggcacccg cgacaatttc atttaattca ttcgtaatac ttgcttgacg agctttgtta
     3061 tacactaact gtaactcatt aatcagatta cctgcgttat cggttgctgc tttcattgcc
     3121 accattcgag cagcctgttc agacgcaaga ttatctacca ctgcctgata aacttgagat
     3181 tctaaataac gaaccagtaa gctgtctaat aacgcttgtg gatttggttc atagagataa
     3241 tcccaagaac cagtttgctc taatgagtca ttttccagtg caggcaaagg aagcaactgt
     3301 tgtactgtcg gtttttgtga catcgtgttt ataaaacggt tatataccac atacacttca
     3361 tcaatttcgc cttcacggta ggcatttacc ataccattaa caataccgac taaatcttcc
     3421 attgccggtg tatcacccaa tccattgata tgacctttaa tctctagccc cattgggtta
     3481 aaaaaggaga tccctttatt tcccaccaca gcaagtcgag acttaacacc ttggtcatct
     3541 ttttctttca attcatgtaa aacggtttta aataaattga tatttaagcc accacataaa
     3601 ccgcgatcgg ttgaaactac taaatagcca acttttttta ccggacgtgg ggtcaaaaat
     3661 gggtgtttat actcaatgtt acctttggca atatggctaa ttgccttgcg aatactttca
     3721 gcataagggc gagaagccgc catacgctct tgggttttac gcatttttga tgcggcaacc
     3781 atttccatcg ctttggtaat tttttgtgta ttacgaacac ttgcaatttt ggttcttatc
     3841 tctttagcac ctgccatatt atttctccgc taaaaagtta gattaccaag cactgttctt
     3901 tttgaaacca gataaaatct cgttcaattt atctttaatt tcatcgttga aattgccgga
     3961 ttttgacagt tcttgcataa actcgccgta gttactttga gcataagcta ataagtttgc
     4021 ttcaaatgag ccaacacgct caaccggaat atcatctaag taaccgtatt cagctgctgc
     4081 taatgataaa cctaattgtg caacagacat cggctcgaat tgtttttgtt tcaacaattc
     4141 agttactttt tgaccgtgtg aaagctgttt acgtgttgct tcatcaagat ctgatgcgaa
     4201 ctgggcaaat gccgctaatt cacggtattg agcaagtgcg gtacggatac caccggcaag
     4261 cttcttaacc agtttagttt gtgcagaacc acctacacgg gaaaccgaga tacccgggtt
     4321 taccgcaggg cgaataccgg cattgaataa gcctgattct aagaaaatct gaccatcggt
     4381 aattgaaatt acgttagtcg gtacgaaggc tgaaacgtca cctgcttggg tttcgatgat
     4441 cggtaatgcg gttaaagaac cggtttgacc tttcacagca ccgttagtga aacgttctac
     4501 atagtctgca tttacacgag acgcacgctc taataagcga gagtgtagat agaacacatc
     4561 acccgggaat gcttcacgac ccggtggacg gcgtaataac aatgaaattt gacggtaagc
     4621 aactgcttgc ttagacaaat catcgtaaac gatcaacgca tcttcaccac ggtcacggaa
     4681 gtattcaccc attgcacaac ctgcataagg tgcaagatat tgtaatgcag cagattcaga
     4741 cgcagaagcc actacaacga tagtgttcgc taatgcaccg tgttcttcta atttacgcac
     4801 cacgttggcg atagttgatg ccttttgacc gattgcaacg tagatacatt taatgcctga
     4861 atcacgttgg ttgatgatcg catcaatcgc taatgcggtt ttaccggttt gacggtcacc
     4921 tataatcaat tcacgctgac cacgaccgat tggtaccatt gagtccaccg ctttgtaacc
     4981 ggtttgaacc ggttgatcaa ccgattgacg gtcgataacg cccggtgcga tcacttcgat
     5041 tggtgagaaa ccgtcatttt ggatttcgcc tttaccatcg attggctgac ctaaggtatt
     5101 tacaacacga cctaataagc cacgaccaac cggcacttct aaaatacgac cggtacattg
     5161 tacttgcata ccttctgcta aatcggcata aggtcccatt acaactgcac caacagaatc
     5221 tctttctaag tttaatgcaa tcgcataacg attgcccggt aatgcaatca tttcaccttg
     5281 cattacatca gataagccgt ggatacgaat aaccccgtca cttaccgaaa cgattgtccc
     5341 tgtgctttga gcttcactca ccacattaaa ctgggcaata cgttttttaa tcaattcact
     5401 aatttcagtt gaatttagtt gcatttttta ttcctcttac aagctcaact cttgactcaa
     5461 gcggtttagc tgtccacgac tactaccatc aatcactaca tcatcataac gaatgatcac
     5521 acccgcaatt aatgaactat ctattgatga agtaatacgt actttcgttc cgagtcgctt
     5581 ttccatcgca ttggcaattt ttgcttcttg tgcatctgtt aatttatctg cagaaacaac
     5641 ctcaacatct ttaattgatt cctgttctgc tcttaattct aaaaatgcat tcaataccgc
     5701 aggtaatact gctaaacgct tgttatcagc cattacgcga atgaaatttt gcccatattg
     5761 atctaattgt tcgccacaaa tcttgataaa cgtctcagaa atctgaccgc ttgcaagtga
     5821 tgagttaaga aaactagcta cttgctcatc gttagcgacg gcggcagaaa aagttaacat
     5881 ctgctgccat tgttccaatt gaccttgttc taaagcaaaa tcgaaagccg ctttagcata
     5941 ggggcgagct actgtgctta attctgacat ctgcccctcc tagttatagt tccgcaacta
     6001 gcttatcaat aatatcgttg ttcgcagcgg catccaccga acgacccacg attttttctg
     6061 caccagcaat agccaatgca gccacttttt gacgaagctc ttcttgaacg cgtttacgtt
     6121 cgctttcaac ttccgcgtaa ccctgttcaa taatacgttg acgttccact tcagcttctg
     6181 cttgtacaga ttctaaaatt tcattacgac gttttgttgc taaatcaatg atattttgtg
     6241 cttcttcttt cgctctaagg atttcttgat ccgcagccgc tttagaatca gcttgttctt
     6301 gtttcgcttt ttcggcagaa gccaatgcgc tagcaatatt tgcctgacgc tcttctatca
     6361 ctcgaattag cggtggccat acaaacttca tacagaacgc aacaaaaagt gcgaacgcaa
     6421 tcagttgacc aattagtgtt gcatttaaat tcacaacgcc ctcctaaaag ccggtaaagt
     6481 tattcaactg aataacggtt aatcaaatcc cgacttattt caataaatca atgaacgggt
     6541 ttgcgaagat gaatagtaat gaaataccta cagcgatcat tgcgatagca tctaaaagac
     6601 cagcaacgat aaacatttta gtttgtaagc ttgatgctaa ttccggctga cgtgcagatg
     6661 actctaagaa tttgccacct aagatagcaa agccgatagc agtaccaaga gcagcgaaag
     6721 ctaataaaat tgaagcacca ataattgttg ctgtgattac tgattccata atgttctcca
     6781 ttagattgag gaattagccc gaaggctggg ttgttgataa aaatacatat caagcggtca
     6841 atattttgca aaaatttgcg aaaaacctac cgcttgtaag gctagtttaa tgatccgctt
     6901 tgttataagc gatactcaaa taaacaatcg ttagcatcat aaaaataaag gcttgaagcg
     6961 taataactaa aatatggaaa atcgcccaaa ccaagtgtaa tggaatcccc aacgcctgta
     7021 gagcgaagtt gtctgccata tacattactg caataaggat aaagattaac tcacctgcat
     7081 acatattacc gaataaacgg aacgccaatg aaatcggttt tgccaaaagc gttaccgttt
     7141 caagaataaa gtttaccgga atgaatgccc agtggttaaa cgggtgtaac gtgtattctt
     7201 tcactaaacc gccaaaacct ttcgatttca ccgtgtaaaa taaaattaac gcaaatacac
     7261 aaatagacat acccagtgtt gcactaatat ctgccgttgg tactgcacgc aaatagtgaa
     7321 taccaaacag attggctaac tgtggtaaga aatcgaccgg gataaggtcg atcgcattca
     7381 taataaacac ccaacagaaa atcgttaatg ctaatggtgc aattacattg cgaggtccgt
     7441 ggaagttatc cttcactaaa ccgtttaccc actcaataac aatctcaaca aaacattgca
     7501 attttcccgg aacgcccgtt gttgcatttt ttgctacacg cgagaacacc cataaaaaaa
     7561 tcgcacctgc cactaaagag aagaacagtg tatctaaatg cactgcccaa aagccttcac
     7621 ctgtcgataa aaaactcaag tgatggctaa tatattctgc tgtcgttcca gccataacat
     7681 atccttttaa attaaaatta gtggtgatgt ttgcttaata caaacgggat catattattt
     7741 aaaaataatg cgataaaata acccacgaaa aacaacacaa catctagagc agggataagt
     7801 ttaaaacagc cgatcaccaa aataatagta accaaccatt tcattccttc accacgatag
     7861 aaagcggtca tttttgactg attttttgca gatttcttaa agaaaaccca atatacaaac
     7921 acaagatgag gcaaaaaact cgctaaagca ccgcctagaa aggaaaaatt aaaattcatt
     7981 ttccataaag acactccaag tgcaaaaacg cacaagagaa aaaattcaac ctttaatgct
     8041 tgtttatatt gtgattttgc tctattgatt acagctgaca cattttatcc tttttgaata
     8101 agtaaaccac acaatcaggc taataattag ccactgttga atcaaagaaa atccgtgcta
     8161 aattatactt tacaaatgaa ttgttgc
//
LOCUS       DANLUQ010000115         8051 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.116, whole genome shotgun sequence.
ACCESSION   DANLUQ010000115 DANLUQ010000000
VERSION     DANLUQ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8051
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.116"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(52..1092)
                     /locus_tag="PXB27_001909"
     CDS             complement(52..1092)
                     /locus_tag="PXB27_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL5346668.1"
                     /translation="MQTTLSPQQIAKKHRTIEKKRSFLLLFFLLICLLSLIFDIMTGP
                     SLLPFNEVWKALFQLGEIEETTKVIVYELRLPMAIMALVVGAALGVGGAEIQTLLNNP
                     MASPYTLGLAAAAGFGASIVIAFGGFGLPTEISVPVGAFVMTLFSSAILFAFSSLGRF
                     SSAILVLVGIALLFLFQSLLSLVQFISAPEVSQQILFWLFGNLTKATWQTISIAFGVT
                     TVCILLLFKDAWKLTALRLGEARALSLGINLTWLRMKTLLIVSVMTATCISFVGIIGF
                     IGLVAPHIARMLLGEDQRFFLPGSMLVGAAFLSMSSVLSKSLVPGALFPVGIITALVG
                     VQFFFWIVLAKR"
     gene            complement(1181..1771)
                     /locus_tag="PXB27_001910"
     CDS             complement(1181..1771)
                     /locus_tag="PXB27_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein phosphodiesterase"
                     /protein_id="HDL5346669.1"
                     /translation="MNFLTHSLISLEIDTQTKQQTLYGNFAGDFYKGLIPHLNLADNL
                     KSGVVLHRSIDKISDRPDSLTTQLLQGTFGRYKGIVSDIMIDHFLAKHFQRLFAKDLN
                     EVQAQIFQQIEQQRTIFPEQFVPMFNWLQSRKALTHYADLDFLEQVFLGMSSRVKKGE
                     ILRNAVTEIWQNYTLLEEAAIQEFFTVKDRATRELS"
     gene            complement(1772..3145)
                     /gene="modF"
                     /locus_tag="PXB27_001911"
     CDS             complement(1772..3145)
                     /gene="modF"
                     /locus_tag="PXB27_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HDL5346670.1"
                     /translation="MVVDGNGSGKTTFSLALQGKISLYSGDYGNSFQHIQLLSFEQQQ
                     KMLEQIFKDRNNDSVSPDDFGLTARQVILNGSPKEALCEEYAHKLHIQHLLERPFMHL
                     STGESRKVLFCQMLVSEPDLFILDEPFEGLDQQSVQYWQQLIAELQSKMALVLIVNRF
                     NDIPDAANHIELLDNLSLILQGERAEIEQQAVYSQLKFAEENVNVPLPDSAAPLLSLP
                     ENINPFELENVNIQYGEKKILDNLSWTVQPKQHWWIKGPNGAGKSTLLSIITGDHPQS
                     YANKVRLFGKQRGSGETIWDIKKNIGYVSSQLHMDYRVNCSAMEVILSGFFDSIGVYQ
                     QVPNALQLKAMEWLERLNLSNVAKKPFRSLSWGQQRLLLITRAMVKHPSILILDEPLQ
                     GLDGINRKLVKQFIEQLVTNSQTQLLFVSHQDSDAPNCITHLFEFVPKGETNEFGFEY
                     HQTALGA"
     gene            complement(3263..4216)
                     /gene="fmt"
                     /locus_tag="PXB27_001912"
     CDS             complement(3263..4216)
                     /gene="fmt"
                     /locus_tag="PXB27_001912"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDL5346671.1"
                     /translation="MSKLNIIFAGTPDFAAQHLQALLDSEHNVIAVYTQPDKPAGRGK
                     KLQASPVKQLAEAHNIPVYQPKSLRKEEAQAELKALNADVMVVVAYGLILPEAVLNAP
                     KYGCLNVHGSLLPRWRGAAPIQRSIWAGDTETGVTIMLMDVGLDTGDMLHKVTTPIEP
                     NETSASLYAKLAELAPLALLEVLNGLANHTFKAEKQDEALSNYAEKLSKEEAKLDWQL
                     PAVQLERNIRAFNPWPFSFLTLTVDGVEQSIKIHQATVLPHQEKAAGTVINVDKTGIQ
                     IATQQGVLNITQLQPAGKKPMSVQDFLNGRADWFVSGTQLS"
     gene            4331..4942
                     /locus_tag="PXB27_001913"
     CDS             4331..4942
                     /locus_tag="PXB27_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDL5346672.1"
                     /translation="MEFFIPKADTIFEEEIKKSRFITYLRHTEGMEQAKAFWQELKIQ
                     HPHARHWCWATVAGLPNDSQQYGFSDDGEPSGTAGKPMLNYLLGSGLGEITAVVVRYY
                     GGIQLGTGGLVKAYGNGVQQALLQLERIRKVLRKNYRLQCEYEQFNTISHLISEKDIV
                     IVEQLFSDKVDLLLAFNPSEVEAFADELTQRFSGKLALIKEEI"
     gene            4946..6430
                     /locus_tag="PXB27_001914"
     CDS             4946..6430
                     /locus_tag="PXB27_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter"
                     /protein_id="HDL5346673.1"
                     /translation="MQFFSIIRIVGILVMCFSFTMLVPATVALIYGDGGGRAFLEAFA
                     LNFMVGTLLWWLCRDNKNELRSREGFLIVVLFWVVLGLLGAVPFIILENPDLNVAESI
                     FESFSGLTTTGATVITGLDSLPKAILFYRQFLQWLGGMGIIVLAVAIIPLLGIGGMQL
                     YRAEMPGPLKEQKMRPRIAETAKALWLIYLSLTILCAVAFWLAGMTVFDAISHSFSTV
                     SIGGFSTHDASIGYFNNAAISYITVFFLLLSACNFALHFAVIDRIRDAQSRKKGGILS
                     RLYWGDYEFRFFLMVQLGLFLVCTLVLVGYGYFDNTSVTIEQALFQSVSISTTAGFTT
                     NDFSQWSSFLPTLLVLSSFIGGCAGSTGGGLKVIRVLLLYLQSKREIHRYIHPNVIQP
                     IKLGNHVLSERVVDGIWAFFSAYFFVFVICWLATIACGMETFDALNAVIASLNNLGPA
                     LGSVSSNFTQVPDSAKLVLTFAMVCGRLEVFTLLVILSPVFWKD"
     gene            6431..6958
                     /gene="hemG"
                     /locus_tag="PXB27_001915"
     CDS             6431..6958
                     /gene="hemG"
                     /locus_tag="PXB27_001915"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HDL5346674.1"
                     /translation="MKTLILYFTTDGQTKKIAERLADVITHEVELISLKDQAVDFAEK
                     IANADQIVIGASIRYGHFNPLVYQFVEQHYPALNAKKTAFYGVNLTARKDNRNTPETN
                     TYVRKFLAKVKWKPTHIEVIAGALLYLRYKPFDRLMIRFIMKITKGETDTSKEYEYTD
                     WQQVENFGKLLANQL"
     gene            7090..7485
                     /gene="mscL"
                     /locus_tag="PXB27_001916"
     CDS             7090..7485
                     /gene="mscL"
                     /locus_tag="PXB27_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDL5346675.1"
                     /translation="MSILKEFREFAVKGNVVDMAVGVIIGGAFGKIVSSLVSDVVMPP
                     IGWLIGGVDFKDLAIEIAPAKEGAEAVMLKYGAFIQNVFDFLIIALAVFGMVKVINSL
                     KKAPVVEEAPAEPTAEEKLLTEIRDLLKK"
     gene            complement(7598..7861)
                     /gene="rpsT"
                     /locus_tag="PXB27_001917"
     CDS             complement(7598..7861)
                     /gene="rpsT"
                     /locus_tag="PXB27_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDL5346676.1"
                     /translation="MANIKSAKKRAVQSEKRRQHNASQRSMMRTFIKKVYAAVAAGDK
                     AAAQTAFVEMQKVVDRMASKNLIHANKAANHKSKLAAQIKKLA"
BASE COUNT         2228 a         1606 c         1800 g         2417 t
ORIGIN      
        1 taagtttcca cgcttaatag ttacatcgtt taagttgagc atagaaaatc cttatcgttt
       61 cgccaataca atccagaaaa agaactgcac accgactaat gccgtaataa taccgaccgg
      121 aaataatgcg cccggcacaa ggctttttga tagcacggat gacatcgata aaaatgccgc
      181 acccactaac attgaaccgg gcaggaaaaa gcgttggtct tcgcctagca acattcgggc
      241 aatatgcggg gcaactaagc caataaagcc gataatgcct acaaagctaa tgcaggtggc
      301 cgtcattaca gacacaatca gcaatgtttt cattcgtaac caagttaagt taatgcctaa
      361 gctcaatgct cgagcttcgc ccaaacgcaa ggcagttaat ttccacgcat ctttgaataa
      421 taataaaata cagactgtag ttacgccaaa ggcaattgag atagtctgcc aagtagcttt
      481 agttaaatta ccgaatagcc aaaacagaat ttgttgggat acctcaggtg cagaaataaa
      541 ttgcaccaat gacagtaaag actgaaataa aaacaataag gcaattccta ccagcactaa
      601 tattgccgag ctaaaacgtc ctaaagagga aaaggcaaat aaaatggcgg aagaaaatag
      661 tgtcatcaca aaagccccga caggtacaga aatttcggtg ggtaaaccaa atccaccaaa
      721 ggcaatcaca atcgaagcac caaagccggc tgcagctgct aaacccaatg tataagggct
      781 tgccattgga ttattaagca aagtttgaat ttcagctccg ccaacaccaa gtgctgctcc
      841 aaccaccaat gccattattg ccattggtaa gcgtagctca tagacaatca ctttggtggt
      901 ttcttcaatt tcgcctaatt ggaataaggc tttccaaact tcattaaacg gaagcaggga
      961 agggcctgtc ataatatcga aaatgaggct gagtaaacaa atgagtaaga aaaagagtaa
     1021 taaaaaactg cgtttctttt caatagttcg gtgcttttta gctatttgtt ggggagagag
     1081 cgttgtttgc ataaaaatgt aatgtttgag aattattatc agacaggata agagaaaaga
     1141 tcgataaatt caagaatatc caagataccg actaaagtat tcaactaagt tctcgagtag
     1201 ccctgtcttt tacagtaaaa aactcctgaa tagcggcttc ttctaaaaga gtataattct
     1261 gccaaatttc ggttacagca tttcttaaaa tctcaccttt cttaactctt gatgacattc
     1321 ccaagaatac ctgttctaag aaatctaaat cggcataatg agttaatgct tttcgtgact
     1381 gtaaccagtt aaacatcggc acaaattgtt caggaaaaat cgtgcgttgt tgttcgattt
     1441 gctgaaagat ctgtgcttgt acctcattta aatctttagc aaataaccgt tggaaatgtt
     1501 tagcgagaaa atggtcgatc ataatatccg acacaatgcc tttataacgc ccgaatgtgc
     1561 cttggagtaa ttgggttgtg aggctgtccg gtcggtccga aatcttatca atcgagcggt
     1621 gtaacaccac accgcttttt aagttatccg ctaaattgag atgcggaatt aaacctttat
     1681 agaaatcacc ggcaaagttg ccatataggg tctgttgctt ggtttgggtg tcaatttcaa
     1741 gtgaaatcag cgaatgggta agaaaattca tttatgctcc aagtgcagtt tggtgatact
     1801 caaacccaaa ctcgttggtt tcgcctttcg gtacaaactc aaataaatgc gtaatgcaat
     1861 ttggagcatc gctgtcttgg tgcgaaacaa aaagtagttg cgtttggctg ttagtcacta
     1921 attgttcaat gaactgtttg actaatttgc ggttaatgcc gtctaaacct tgtaacggtt
     1981 cgtctagaat cagaattgat gggtgtttca ccattgctcg ggtaatcaaa agtaagcgtt
     2041 gttgccccca cgagagagag cgaaacggct ttttggcaac attgcttaaa ttgaggcgtt
     2101 ccaaccattc cattgctttt aattgcaagg cattcggcac ttgttgatac acgccgatgc
     2161 tgtcaaaaaa gccagaaaga atgacctcca ttgcggagca atttacccga taatccatat
     2221 gcaactggct gctgacatag ccgatatttt tcttaatatc ccaaatggtt tcgccacttc
     2281 cccgttgttt gccaaatagt cggactttat tggcataaga ttgtgggtga tcgccggtga
     2341 taatcgaaag caaggtcgat ttcccagctc cattcgggcc tttaatccac cagtgttgtt
     2401 taggctgaac cgtccaactt aggttatcta aaatcttctt ctccccatat tgaatattca
     2461 cattttccaa ctcaaacgga ttgatgtttt ccggcaggct gagtagcgga gctgcactat
     2521 caggcagtgg cacattgaca ttctcttctg caaattttag ctgagaatag accgcttgtt
     2581 gttcaatttc tgctcgctct ccttgcagaa ttaatgataa attatctaat aactcaatat
     2641 gattggcagc atcaggaata tcgttaaaac gatttacaat cagcactaac gccattttgc
     2701 tttgtagttc ggcaatcagt tgttgccaat attgcaccga ctgctgatcc agcccttcaa
     2761 acggttcgtc taaaatgaat aaatccggct cgctcactaa catttggcaa aatagcactt
     2821 tgcggctttc accggtggaa aggtgcataa acgggcgttc aagcagatgt tgaatatgca
     2881 atttgtgggc atattcctca cataacgcct cttttggact gccatttaaa attacttgtc
     2941 gtgcggttaa gccgaaatca tcgggcgaga cactatcgtt gttccgatct ttgaaaatct
     3001 gctccaacat tttttgttgt tgctcgaaag agaggagctg aatatgttgg aacgaattac
     3061 catagtcgcc ggaatagagc gagattttac cctgtaaagc gagtgaaaaa gttgttttgc
     3121 ctgaaccgtt tccgtctacg accacctaaa agtcattggg ttggatctgt aaggcgtcaa
     3181 tcgtgagttt atgttgttta accagtgaaa atcgggcatt ggttattgta agtgaaggct
     3241 gtgtttgcat atatttcccg ttttaactta gttgtgtccc gctgacgaac caatccgctc
     3301 gaccgtttaa gaaatcttgc accgacattg gttttttacc cgccggttga agttgggtaa
     3361 tattcagcac gccctgttgg gtggcaattt gaatcccggt tttatccaca ttaattaccg
     3421 tgccggctgc tttttcttgg tgaggtaaca ccgtggcttg gtgaattttg atactttgct
     3481 caacgccatc aacggttaag gttaaaaaac taaacggcca agggttaaaa gctcggatat
     3541 tgcgttccag ttgaactgct gggagttgcc aatccaattt agcttcttct ttggaaagtt
     3601 tctcggcgta attggacagt gcttcatctt gtttttccgc tttgaatgtg tggttggcta
     3661 aaccgtttaa cacctcaagc aatgccagag gggcaagttc tgccagtttg gcatacaagg
     3721 aggcggaggt ttcattcggc tcaatcgggg ttgtgacttt atgtaacata tcaccggtgt
     3781 ctaagccaac gtccattagc ataatggtta cgccggtttc tgtatcacct gcccaaatcg
     3841 aacgctgaat cggagctgct ccacgccagc gaggtagtag cgaaccgtgt acgtttaggc
     3901 aaccatattt aggagcattc aatactgctt caggtagaat taagccgtaa gctaccacca
     3961 ccatcacatc ggcatttagg gctttcagtt cagcttgagc ctcttcctta cgcagggatt
     4021 tgggttggta aacaggaatg ttgtgagctt cggccagctg ttttaccggg ctggcttgca
     4081 gttttttgcc tcgcccggca ggtttatccg gctgagtata aaccgcaatc acgttatgct
     4141 cggaatctaa taaggcttgt aaatgttgag cggcaaaatc aggcgtgccg gcaaaaataa
     4201 tattgagttt tgacatattt tttattcgtt caagttatca atgaaagact tattaaacca
     4261 ttaaacagca caaataaaaa gtggcttgaa tatggtatca ttgccacatt caagataaga
     4321 gaatcaaaca atggaatttt ttatccccaa agcggataca atttttgaag aagagattaa
     4381 aaaaagccga tttatcacct accttcgcca tactgagggt atggagcagg ccaaggcatt
     4441 ttggcaggag ctgaaaattc agcatccgca cgcacgccat tggtgttggg caacggtggc
     4501 aggcttgccg aatgattcgc aacaatacgg cttttcggat gatggagaac cgtctggtac
     4561 agcaggtaag ccaatgctca attatttgct tgggtcaggc ttgggggaaa ttacagcggt
     4621 ggtggttcgc tactacggtg gtatccagct tggcaccggc ggcttggtga aagcctatgg
     4681 caatggggtg caacaggcgt tattgcagtt ggagcggatt cgcaaagttt tgcgaaaaaa
     4741 ctaccgcttg caatgtgaat atgagcagtt taataccatt tctcatctca ttagcgagaa
     4801 agatattgtg attgtcgagc aactttttag cgataaagtg gatttgttgc tggcatttaa
     4861 cccgagcgag gttgaggcct ttgcagatga attaacccaa cgcttttcgg gcaagctcgc
     4921 attaataaaa gaagaaatct agtctatgca atttttttcc attattcgta ttgttggcat
     4981 tttggtgatg tgtttttcat tcacaatgct tgtgccggca acggtcgccc taatttatgg
     5041 cgatggcggt gggcgggcgt ttttagaagc cttcgctctg aattttatgg tcggtacgct
     5101 gctttggtgg ctgtgccgtg acaacaaaaa cgagctacgt tcccgtgagg gctttttgat
     5161 tgtagtactg ttttgggtgg tgctgggttt gctcggggct gtgcctttta ttattttgga
     5221 aaaccccgat ttaaacgtgg cggaatccat ttttgaatcc ttttccggct taactacaac
     5281 gggtgcaacc gtcatcaccg gcttagatag cttgcccaaa gcgattttat tctaccgcca
     5341 atttttgcaa tggctagggg gaatggggat cattgtgtta gcggttgcga ttattccgct
     5401 gttaggcatt ggtggaatgc aactttatcg tgcggaaatg cccggaccgc tcaaagagca
     5461 gaaaatgcga cctcgcattg cggaaacggc aaaagcgttg tggctgattt atctcagcct
     5521 gacgattctg tgtgcggtcg cattttggct cgccggaatg acggtgtttg atgccatttc
     5581 gcatagcttt tccacggttt cgattggcgg attttctacc cacgatgcca gcatcggcta
     5641 ttttaataat gcggcaatta gctatattac cgtgtttttc ctgctacttt ctgcctgcaa
     5701 ctttgcattg cattttgcgg tgattgatcg catacgagat gcacaatcac gcaaaaaagg
     5761 gggtatttta agccgcttgt attggggcga ttacgagttt cgttttttct tgatggtgca
     5821 gttagggctt ttcttggttt gtaccttggt gttagtgggt tacggttatt tcgataatac
     5881 ctctgttacg attgagcaag cgttgtttca atcggtgtcc atttccacca cggccggctt
     5941 tactaccaat gattttagcc aatggagttc gtttttaccc acgctgttgg tgctgtcttc
     6001 atttatcggt ggttgtgccg gctcaaccgg tggcggcttg aaagtgattc gggtgttgct
     6061 gctttattta caaagtaaac gtgaaatcca ccgctacatt caccccaatg tgattcaacc
     6121 gattaaatta ggcaaccacg ttttatcgga acgggtggtt gatggtattt gggcgttttt
     6181 ctcggcgtac tttttcgtgt tcgtgatctg ctggcttgct accatcgctt gtggaatgga
     6241 aacctttgat gctctaaatg cggtaattgc ctcactaaat aatctaggtc ctgcattagg
     6301 gagcgtgagc agcaatttca cccaagtgcc ggacagtgcc aagttagtgc tgacttttgc
     6361 aatggtatgc ggacgtctgg aagtgtttac cttgctggtg attttaagcc ctgttttttg
     6421 gaaagattaa atgaaaacct taattctcta ttttacgacc gatggtcaaa ccaaaaaaat
     6481 tgccgaacga ttggcggacg tgattactca cgaagtcgag ctgatttcgc taaaagatca
     6541 agcggtcgat tttgccgaaa aaattgcaaa tgccgaccaa attgtcatcg gtgcttcaat
     6601 tcgctatggg cattttaacc cgttggttta tcagtttgtg gaacagcatt atccggcatt
     6661 aaacgccaag aaaactgcct tttacggtgt gaatttgacc gctcgcaaag ataatcgaaa
     6721 cacgcctgaa accaatactt atgtgcgtaa atttttagct aaagtgaaat ggaaaccaac
     6781 ccacattgag gtaattgctg gggcattact ttacctacgc tataagccat tcgaccgatt
     6841 gatgattcgc tttattatga aaattaccaa aggcgaaacc gatacctcca aagaatatga
     6901 gtacaccgat tggcagcagg tggagaattt tggcaaatta ttagccaatc agttataatc
     6961 aaactgatct aaatcaaaaa aatgtaaatt tatttaaatg ttgtagaaat tttaaagatt
     7021 ttgattataa tcgccagccc acgttcttgc gaagaccgtg tttaatcatt gaatgaaagg
     7081 aaagccccct tgagtatttt aaaagagttc cgtgaatttg cggtaaaagg taatgttgtt
     7141 gatatggctg tcggtgtgat tatcggcggt gcattcggta aaattgtttc ttcattagtt
     7201 agcgatgttg taatgccacc tatcggttgg ttaatcggtg gcgtggattt caaagattta
     7261 gccattgaaa tcgccccggc aaaagaaggt gcggaagcag tgatgttaaa atacggtgca
     7321 tttatccaaa acgtatttga tttcttaatt atcgcacttg cggtattcgg tatggtgaaa
     7381 gtgattaact cactgaaaaa agctccagtt gtagaagaag ctcctgcaga gccaacagcg
     7441 gaagagaaat tattaactga aatccgtgat ttattgaaaa aataattaac tgattttgaa
     7501 acaagggcta ttttagaata tctagaatag ccctttgtgt tatggttaaa aagaaaaaaa
     7561 ccaacataaa gtcggttttt ttcatatctg ttcgatatta cgctaatttt ttgatttgcg
     7621 ctgctaattt agatttgtgg ttagccgctt tgttagcgtg gattaagttt ttagatgcca
     7681 tacggtctac aactttttgc atttcaacga aagccgtttg agctgctgct ttatcgcctg
     7741 ctgctactgc tgcatatact tttttgatga atgtacgcat cattgagcgt tggcttgcgt
     7801 tgtgttggcg gcgtttttca gattgaaccg cacgtttttt tgctgacttg atattagcca
     7861 aggtcaaact cctaaaatat ctgtgtaaaa attagatata aacaaaatcg ttgtttatat
     7921 tgcctatcga tagtttttaa ttcattttca acgagacacg tgcttgaaaa taacaccgtt
     7981 tttctgatag aaaacgatag ggcgagattt taacagtttt tttctgattt tcctataaca
     8041 aactgctaaa a
//
LOCUS       DANLUQ010000116         7928 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.117, whole genome shotgun sequence.
ACCESSION   DANLUQ010000116 DANLUQ010000000
VERSION     DANLUQ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7928
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.117"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            50..1051
                     /gene="pdxA"
                     /locus_tag="PXB27_001918"
     CDS             50..1051
                     /gene="pdxA"
                     /locus_tag="PXB27_001918"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HDL5346677.1"
                     /translation="MKPILGITMGDAAGIGPEVIIKALANQRIYELAHPVVIGDRKII
                     QRALPIVHSELKLRILNNLDNFVAEYGVIDILDLNNLPEDLPFAQVDPRAGKAAYEYI
                     EEAVNLAMGGKIHAIVTAPLNKEALHAGGKPFPGHTEILATLSNTKDYSMMLTSEKLN
                     VIHVTTHVQLRKACDLITKERVATVIDLAEENARILGFKEPRIAVAGLNPHSGENGMF
                     GDEDQKEIVPAIQEAQAKGFNVTGPVPPDTLFHRAANLNEFDIVVVMYHDQGHIPIKL
                     LGFDTGVNVTVGLPFIRTSVDHGTAFPIAGKGTADSRSMTEALYLGAKMANIKYATH"
     gene            1062..2060
                     /locus_tag="PXB27_001919"
     CDS             1062..2060
                     /locus_tag="PXB27_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HDL5346678.1"
                     /translation="MSTQTSKSIVPIYDGMNKIPGGAMVIPLILGSIIGTFFPEFLNL
                     GSFTTALFKSSAGPLIALLIFATGMQITVRSSGPVLAHTGVVLLFKTIIPALLVVILG
                     LWVGNDGVMGVSLLAMLTVVANSNGGIWLAFTGKYGDYRDKGAYVASAVNDGPFFVLL
                     FLGVSGLADIPLSLMLAAVIPLIIGMVIGNLDPKWTELMKPTGAIVIPFFAFALGTGI
                     NLQSIITGGATGIILGIVASLFTGFLVFIGYKTILRRGNQSGIGFAAGTTAGNAIITP
                     EIVAQADPTLAPFVQTATAQIAAAVLVSAILAPLFAAWVLKRQGGLEQDTIKGENL"
     gene            2057..3283
                     /locus_tag="PXB27_001920"
     CDS             2057..3283
                     /locus_tag="PXB27_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HDL5346679.1"
                     /translation="MKETLLVIADDLTGANDTAVMFAESGFDTVLKTKVSALAQIHPD
                     KAQVISVSTDSRAIGEKAKELTQIAISNAIQNSIGQIYLKIDSTMRGSVKYQIEGAIK
                     AWAGLYPDVKAIICPAYPEMGRTIEAGHLYVNGVPVNETASGKDPICPVHSSSMQKLL
                     PDAQVIPCDTVDNLQASIENSEYKQIVIDAKTHEDLSMIAQVINHIGNKIIPVGSAGL
                     AQKLKNIQNQQQPERNLKLGRALIVVTSIHETSQLQVDEYISTLGGKSIVFNPSPSQL
                     INYSQSEHSLKLQLNALIRFSRDHVIIRANPAKVVNAKSANITETSKEISQHLADLSK
                     FCLDNEKFDSLILFGGDGAAALLDQMNVTEMNVQYAVVPGVPLCSIEKGNYSGITVMT
                     KSGGFGNNTLLSNIMN"
     gene            3391..4146
                     /locus_tag="PXB27_001921"
     CDS             3391..4146
                     /locus_tag="PXB27_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL5346680.1"
                     /translation="MSKNRLEQILLFLKNHNIATVEQLVKVTEASPATIRRDLIKLDE
                     EGVVIRTHGGVSLNQFIPFQPTTNEKQFKQIVEKERIAEYAVSLISPGNSILLDAGTT
                     TLCIAKKIAHMPLRVITTDLHIALLLSEYKQIELIITGGRVDNSSQSCIGNHSLTLLE
                     NINPDIAFVSCNSWSLEKGVTAPTEEKANLKRMLQQNAKQKVLVADSSKYGKYSLFRA
                     TQLDNFTQIITDKGLPIEYSQALSSNDFKLSLV"
     gene            complement(4227..4397)
                     /gene="rpmG"
                     /locus_tag="PXB27_001922"
     CDS             complement(4227..4397)
                     /gene="rpmG"
                     /locus_tag="PXB27_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005624479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL5346681.1"
                     /translation="MAAKGAREKIRLVSTAETGHFYTTDKNKRNMPEKMEIKKFDPVV
                     RKHVIYKEAKIK"
     gene            complement(4409..4645)
                     /gene="rpmB"
                     /locus_tag="PXB27_001923"
     CDS             complement(4409..4645)
                     /gene="rpmB"
                     /locus_tag="PXB27_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004997628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDL5346682.1"
                     /translation="MSRVCQVTGKRPAVGNNRSHAMNATKRRFLPNLHTHRFWVESEN
                     RFVTLRLTAKGMRIIDKKGIDAVLAEIRARGEKI"
     gene            complement(4877..5614)
                     /gene="radC"
                     /locus_tag="PXB27_001924"
     CDS             complement(4877..5614)
                     /gene="radC"
                     /locus_tag="PXB27_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDL5346683.1"
                     /translation="MEEKMNDFFDEDSQEPTMMPREKLLTQGAASLTDSELLAIFLRT
                     GTKAVPVLALAESVLMAFGSLRQLLNANIDDFCKIYGLGKTKYIQLQASKEMTKRYLA
                     QQMEFSEMIQAPYMAIMYFQTELEEEEREVFMVLFLDNQNRLIYKEKMFFGTINQTAV
                     HPREIIKRALKYNAAAIIVAHNHPSGSCLPSESDRSLTKKIEMACELVDIRFVDHIIV
                     GKGDYFSFEEEKLEMKEHSFLQISDRK"
     gene            5764..6966
                     /gene="coaBC"
                     /locus_tag="PXB27_001925"
     CDS             5764..6966
                     /gene="coaBC"
                     /locus_tag="PXB27_001925"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDL5346684.1"
                     /translation="MLQNKKILVGISGGIAAYKTIEFIRHLKKANAEVRVVLTPAAEA
                     FVTPLTLQAISGNAVSTSLLDPQAELAMGHIELAKWADLIVIAPASADFIARLRMGMG
                     NDLLSTLCLATASPILLAPAMNQQMYKQIAVQENLSVLVNRGVQLIGPNSGFQACGDV
                     GAGRMSEPEEIYQAVLAHFSNNQDLEGLNVTITAGPTREAIDPVRYISNHSSGKMGYA
                     IANAFAERGAKVTLISGPVNLPTPKNVERINVESAVEMEQQAVKFAQKSAIFIGCAAV
                     ADYRMAAVSDQKIKKTDANDELTLKLVKNPDIIANVANLAENRPFVVGFAAETQNVAE
                     YAKSKLERKNLDMICANDVSGGQVFGQDQNALHLFWKNGEKTLPLADKSKLAESLVQE
                     IVKHYREK"
     gene            6966..7283
                     /locus_tag="PXB27_001926"
     CDS             6966..7283
                     /locus_tag="PXB27_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346685.1"
                     /translation="MKLLVSHFHQGEGKFPTFSAGTAVRLGSECVNYPNWFACEIDGW
                     KTFVPSHFISEDKLICDYNPTELVAAQGERVEFLELHYQWAIVKRENEIGWLPCEILR
                     VIG"
     gene            7280..7462
                     /locus_tag="PXB27_001927"
     CDS             7280..7462
                     /locus_tag="PXB27_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HDL5346686.1"
                     /translation="MKIMQLTSNQYINDIKHILLSARQKAYQAVNSAMVEAYWKIGER
                     IVQEEKNMVKDQNEKV"
     gene            7449..>7928
                     /locus_tag="PXB27_001928"
     CDS             7449..>7928
                     /locus_tag="PXB27_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDL5346687.1"
                     /translation="MKKYNIVFSADKNYLPYLEIALKSLLAHHNHLSIFILSTGDISH
                     HWIETLRPYLENRYSTLRLGYLDSNNLANFNSNGYITNSTYLRYYIEELFEHSDSPYW
                     IYLDCDLVVNGNITDPFTNLDFSFGSIAAIADPYVNKLKNHPYIYKNYFNAGVFYLNA
                     "
BASE COUNT         2502 a         1499 c         1519 g         2408 t
ORIGIN      
        1 tataagctat acttcgttca aaatgattaa tttcaatcaa ggagtaaata tgaaacctat
       61 attgggaatt acgatgggag atgccgccgg tatcggacca gaagttatta ttaaagcatt
      121 agctaatcaa cggatttatg agttagcaca ccctgttgtg atcggtgatc gaaaaattat
      181 acaacgagcc ttacctattg ttcattcaga attgaaatta agaattctca ataatctaga
      241 taactttgta gccgaatatg gcgttattga tattttagat ttgaataacc tgcctgaaga
      301 tttgcctttt gctcaagttg atccgcgtgc tggaaaagca gcttatgaat atattgaaga
      361 ggcagttaat ttagcaatgg gaggaaaaat ccacgctatt gttacagcac cactgaataa
      421 agaagcccta cacgcaggtg gtaaaccatt tcctggccat acagagatcc ttgcaaccct
      481 cagtaatact aaagattact caatgatgct aaccagtgaa aaactcaatg ttattcacgt
      541 tactactcac gtccaacttc gtaaagcctg tgatttaatt accaaagaac gagttgctac
      601 agttattgat ttagcagaag aaaatgctcg cattcttggg tttaaagaac ctcgtattgc
      661 agtcgctggt ttaaatcctc attcaggtga aaatggaatg tttggcgatg aagatcaaaa
      721 agaaattgtt cccgcaattc aagaggctca agccaaaggg tttaatgtta cagggcctgt
      781 ccctccggat actttatttc accgtgcagc taacctaaat gaattcgata ttgttgttgt
      841 gatgtatcac gatcaagggc atatccctat caaattatta ggctttgata caggtgtaaa
      901 cgttactgtc ggtcttccat ttattcgaac atctgtcgat cacggaactg ctttcccaat
      961 agcaggcaaa ggaacagcag atagtcgtag tatgactgaa gcactctatt tgggtgcaaa
     1021 aatggcaaat atcaaatatg cgacacacta aggagattaa tatgtctact caaacatcta
     1081 aatccatcgt cccgatatat gatggtatga ataaaattcc tggcggtgca atggtaatac
     1141 cgctaattct tggttctatt attggtacat tctttcctga atttttaaat ttaggttcat
     1201 ttacgacggc tttattcaaa tcatcagcag gaccacttat tgcactatta attttcgcta
     1261 caggtatgca aattaccgtg agaagttctg gacctgtact tgcccatacc ggtgttgtat
     1321 tactatttaa aactattatt cccgctcttt tagttgtgat tttaggatta tgggtaggta
     1381 atgatggtgt tatgggcgtt tctttattag ctatgctaac tgttgttgca aattcaaatg
     1441 gtggtatttg gttagcgttt acagggaaat atggtgatta tagagataaa ggagcttatg
     1501 tcgcttcggc tgttaatgat ggacctttct ttgttttact atttttaggt gtatcaggat
     1561 tagcagatat tcctctttca ttaatgcttg cagctgttat tcctcttatt attggtatgg
     1621 ttattggtaa cttagatcct aaatggacag agttaatgaa accaacaggt gcaatagtca
     1681 ttcctttctt tgcttttgca ttaggaacag gaatcaatct gcaatctatt attactggag
     1741 gagcaacagg aattatttta ggtatcgtag cttctctctt tacaggtttt cttgtattta
     1801 tcggttacaa aacaatttta agacgtggta atcaatcagg aatcgggttt gcagcaggaa
     1861 ctactgctgg aaatgccatt attacacctg aaattgttgc tcaagcagac cctacattag
     1921 caccttttgt tcaaactgcg actgcacaga ttgccgcagc tgtattagtt tcagcaatct
     1981 tagctccatt atttgcagcg tgggttttaa aacgtcaagg tggcttagaa caagacacta
     2041 taaaaggaga aaatctatga aagagacact cttagttatt gctgatgacc ttactggtgc
     2101 aaatgatacc gcggttatgt ttgctgaatc aggatttgac actgtcttaa aaactaaagt
     2161 ttcggcatta gcccaaattc atcctgataa agcccaagta atttctgttt ctacagattc
     2221 aagagcaatt ggagagaaag caaaagagct gactcaaatt gcaattagca atgctattca
     2281 aaatagtatc gggcaaattt accttaaaat tgattccaca atgagaggct ctgttaaata
     2341 tcagattgaa ggtgctatta aagcttgggc tggtttatat cccgatgtta aagcaattat
     2401 ttgtcctgca tatccagaaa tgggaagaac tatagaagct ggtcatttat atgttaatgg
     2461 tgttcctgta aatgaaactg cttcaggaaa agatcctatc tgccctgtgc attcatcttc
     2521 tatgcaaaaa ctattgcctg atgctcaagt aatcccgtgt gatacggtcg ataatttgca
     2581 ggcatctatt gaaaatagtg aatataaaca gatcgttatt gatgctaaaa cgcacgaaga
     2641 tctctcaatg attgcccaag tcataaatca cataggtaat aaaattattc ctgttggctc
     2701 agccggattg gcacagaagt tgaaaaatat ccaaaatcaa caacaaccag agcgaaatct
     2761 taaattaggt agagcattaa ttgtcgtaac ctcaattcac gaaacaagcc aactgcaagt
     2821 tgatgaatat atttcaactt taggaggaaa atctattgta tttaatcctt cgccttcaca
     2881 gttgattaac tactcgcaat cagaacattc actgaaacta caattaaacg ctctaatacg
     2941 ttttagtaga gatcatgtta ttattcgggc aaaccctgca aaagttgtaa atgcaaaatc
     3001 tgccaatatc acggaaactt ctaaagaaat ttctcaacat ttagctgatt taagtaaatt
     3061 ctgccttgat aatgaaaaat ttgatagcct tatattattt ggtggagatg gtgcagcagc
     3121 attattagat caaatgaatg taacagagat gaatgtacaa tatgcggtag taccgggcgt
     3181 tcctttatgc tccattgaaa agggcaacta ctcaggcatc actgtaatga ctaaatctgg
     3241 aggatttgga aacaacacac tcttaagcaa tataatgaac tagtaacaac aaagaaccta
     3301 aagcaacaat gttttaggtt ctttaatttt aactatctta tttttaaaaa attacttata
     3361 gtaaaagcag ttaaaaatta gagagtttat atgtctaaaa accgtctaga acaaatttta
     3421 ctattcctaa aaaaccacaa tattgctacc gtagagcaac tggtaaaagt gactgaggct
     3481 tctcctgcaa ctatccgccg tgatttaata aaattagatg aagaaggcgt cgtgattcgc
     3541 acacacggag gggtcagctt aaaccaattt ataccgtttc aaccaacaac aaatgagaag
     3601 caatttaagc aaattgtcga aaaagaacgc atagctgaat atgccgtttc gcttatttca
     3661 ccgggaaatt ctattttatt agatgctggc actacaacgc tatgcattgc gaaaaaaatc
     3721 gctcatatgc cacttcgagt gattacaact gatctacata ttgctttatt actctctgaa
     3781 tataagcaaa tagaattaat tattacaggg ggacgagtag ataatagtag tcaatcttgt
     3841 attggtaatc atagtttaac tttattagaa aatattaatc ctgatattgc ttttgtgagc
     3901 tgtaattctt ggagcttaga aaaaggagtt acagcaccta cagaagagaa agctaattta
     3961 aaaagaatgc tccaacaaaa cgccaaacaa aaagtactgg ttgccgatag cagcaaatat
     4021 ggaaaatact ctttatttcg tgcaactcaa ctcgataact ttacacaaat tatcacagat
     4081 aaaggactac ctattgaata tagccaagcc ttgtcaagta atgattttaa gctatcatta
     4141 gtttaatcat acaagcggtc ttttttcact aattttttgt atatgaaaaa cccagcattc
     4201 gctgggtttt aaatcttaaa acaaaattat ttaatttttg cttctttgta gataacgtgt
     4261 ttacgcacaa ctggatcaaa ttttttgatt tccatttttt caggcatatt acgtttgttt
     4321 ttatctgttg tgtagaagtg accagtttct gctgtagaaa ctaaacggat tttctcacga
     4381 gcgcctttag ctgccatttt ttagctcctt agattttttc gccacgagca cggatttcag
     4441 ctaatactgc atcaatgcct tttttatcaa taatacgcat acctttcgca gttaaacgta
     4501 aggttacgaa acggttttcg ctctcaaccc agaaacggtg agtgtgaagg tttggtaaga
     4561 aacgacgttt agtcgcattc attgcgtgtg agcggttgtt accaactgct ggacgcttgc
     4621 cggttacttg gcaaactctt gacatttgaa tttctccaat aattttaagc ttaaaaatgg
     4681 ttcgggcgat ctcacaaaag gcgttgctca accaacccct ccgattttga ttgcctcgtc
     4741 aggcattaac ccgaccacag tctatgtaat aaagactgcc gattataccg atttcatttg
     4801 ttatattcaa gtagaattta tatgcgtatc ttttactgtt tcaggagaaa tgttaaaggt
     4861 tacataaagc aagcggtcat tttctatcag aaatttgcaa aaatgaatgc tccttcatct
     4921 ctaatttctc ttcctcgaac gaaaaatagt cccctttgcc gacaataata tgatccacaa
     4981 agcgaatatc aaccaattcg cacgccattt cgatcttttt agttaaactt ctgtccgact
     5041 cactcggcag acaagaacct gaggggtgat tatgagccac aataatcgcc gcggcattat
     5101 attttaacgc tcttttaata atttctcggg ggtgaacagc agtttgatta atggtgccga
     5161 agaacatttt ttctttataa atcaatcggt tttggttgtc caaaaacagc accataaaaa
     5221 cttcccgttc ttcttcctcc aactcggttt ggaaatacat aatcgccata taaggggctt
     5281 gaatcatctc gctaaattcc atttgttgag ccagatagcg tttggtcatc tctttagagg
     5341 cttgtaactg aatgtatttg gttttgccta aaccgtaaat tttgcaaaaa tcatcaatat
     5401 ttgcattcag taattggcgt aacgagccaa atgccatcaa cacactctct gccaatgcca
     5461 gcacaggcac agctttcgta ccagtccgta aaaatatcgc taataattcg gaatcggtta
     5521 atgaggctgc cccctgtgtt aataatttct cacgcggcat cattgttggc tcttgagaat
     5581 cttcatcaaa aaaatcgttc attttttcct ccatcaattt agttaagcat aaaagagcca
     5641 acctttgcag acaaagggat ttattcaatt tttcgagcaa gatcgcaaaa atagtgaagt
     5701 tgtgagcaac tccttattcc gctaaaatat gcaaaataaa tccacgcttt aaggatagta
     5761 aagatgttac aaaacaaaaa aattttagtc ggcatttccg gtgggattgc agcctacaaa
     5821 accattgaat ttatccgcca cttaaaaaag gcaaatgcgg aagtgcgtgt agtgctaacc
     5881 ccagcagcag aagcctttgt aacgcctttg accttacaag cgatttccgg caatgcggtt
     5941 tccacttcac tactcgaccc acaagccgaa ctggcgatgg ggcatattga gctggcaaaa
     6001 tgggcggatt taatcgttat cgccccggca agtgccgatt ttattgctcg cctgcgtatg
     6061 ggaatgggca acgatttact ttccactctc tgccttgcga ctgcaagccc gattttgctt
     6121 gcaccggcaa tgaatcaaca aatgtacaaa cagattgcgg tgcaagaaaa tctgtctgtt
     6181 ttagtcaatc gtggcgttca gctcatcggc ccgaatagtg gcttccaagc ctgtggtgat
     6241 gtgggggctg gcagaatgtc tgagcctgaa gaaatttacc aagcggtgtt ggctcatttc
     6301 tccaacaatc aagatttaga gggcttaaat gtaaccatta ccgccggacc gactcgtgag
     6361 gcgattgacc ctgtgcgtta tatcagcaac cacagctccg gcaaaatggg ctacgcgatt
     6421 gctaatgcct ttgccgaacg tggagcaaag gttacgttga ttagcggtcc ggtgaattta
     6481 ccaacgccga aaaatgtgga acgaattaat gtggaatctg cggttgagat ggagcaacaa
     6541 gcggtcaaat ttgcccaaaa atctgcgatt tttattggct gtgcagcggt tgccgattac
     6601 cgaatggcgg cagtctctga ccaaaaaatt aagaaaaccg atgccaatga tgaactcacg
     6661 ctaaaattgg taaaaaaccc cgatattatc gctaacgtcg ccaatcttgc cgaaaaccgc
     6721 ccttttgtgg ttggttttgc cgcagaaacg caaaatgtgg cggagtacgc taaatcgaaa
     6781 ctcgaacgca aaaatctgga tatgatttgt gccaatgatg tctcaggcgg acaagtgttc
     6841 gggcaggatc aaaatgcgtt gcatttgttc tggaaaaatg gtgaaaaaac gcttcctctg
     6901 gcggataaaa gcaaattggc agaaagtttg gtacaggaaa ttgtaaaaca ttatcgagaa
     6961 aaataatgaa attactggtt agccattttc atcaaggcga agggaaattt cctacattct
     7021 cagctggtac tgccgttcga ttagggagtg aatgtgtaaa ctacccaaac tggtttgcct
     7081 gtgaaattga cggttggaaa acctttgtgc caagccattt tattagcgaa gataaattga
     7141 tatgcgatta taacccaacg gaacttgttg cagcacaagg cgagagagta gaattccttg
     7201 aactacatta ccaatgggca attgtcaaaa gagaaaacga aattggctgg ctaccttgtg
     7261 aaatcttaag ggtaatagga tgaaaattat gcaacttacg tcaaatcagt atatcaacga
     7321 cattaaacat attcttttat ctgctcgcca aaaggcgtat caagccgtga attcggcaat
     7381 ggtcgaagcc tattggaaaa tcggtgaacg aattgtgcaa gaagagaaaa atatggtcaa
     7441 agatcaaaat gaaaaagtat aatattgtat tttcagcaga taaaaactac ctgccatatt
     7501 tagagattgc cttaaaatca ctattagctc atcataatca tttatctatt tttatattga
     7561 gtactggcga tatttctcac cactggatag aaacattacg cccctattta gaaaatagat
     7621 attccacatt aagacttggc tatttagatt caaataattt agcaaatttt aactccaacg
     7681 gctatattac taattcaact tacctacgat attatattga agaactattt gaacacagcg
     7741 atagcccata ttggatttat ttagattgcg atcttgtggt taatggaaat attacggatc
     7801 catttacaaa cctagatttt tcttttggaa gtattgctgc aattgctgat ccttatgtaa
     7861 acaaactaaa aaatcatccc tatatttaca aaaactattt caatgctggt gtattttatc
     7921 taaatgca
//
LOCUS       DANLUQ010000117         7907 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.118, whole genome shotgun sequence.
ACCESSION   DANLUQ010000117 DANLUQ010000000
VERSION     DANLUQ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7907
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.118"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            1..3072
                     /gene="fdnG"
                     /locus_tag="PXB27_001929"
     CDS             1..3072
                     /gene="fdnG"
                     /locus_tag="PXB27_001929"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014550636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:586..588,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HDL5346688.1"
                     /translation="MQISRRKFFKVCAGGMAGTSAAMLGFAPATALAAPREYKLLRAK
                     ETRQTCTYCAVGCGMIMYSLGGEAMNSKGKLIHIEGDSDHPVSRGALCPKGAGALDYV
                     NSDRRVHYPEVREAGSSEWKRISWHEAIERIARHMKNDRDENFVTHNEAGLEVNRWMT
                     AGFLAGSACSNETGIVTQKFIRSLGMIFTDNQASIUHGPTVASLAPSFGRGAMTNHWV
                     DIKNSDLVIVMGGNAAEAHPVGFRWAVEAKKQNGAKLMVVDPRFNRSAAVADIYIPLR
                     PGTDIAFLLGAVNYLLKNDKIQHEYVKHYTNATFLLNEGFKFEDGLFTGYDEATRTYK
                     DKSTWAYQLDEQGQPKRDMTMQDPRCVINMLKDHVSRYTPEMVERITGTPQKDFLIFC
                     EEIAKTSAPDKAATFLYALGWTQHTVGAQNIRSMAIIQLLCGNIGVSGGGVNALRGHS
                     NVQGITDLGLFPHMLPGYIRLPTEADATLESYLNAIAPKTLVAGQVNYGQNNPKFAVS
                     MLKTFYGDKATKENEFGYHYLPKYPKGGTDHFRFIEQMYQGKVNGYFCQGMNPIASYP
                     NSQKLIKALSNLKYLVIFDPLITDTSEFWKNHGEFNNVNPSEIQTEVFRLPTTCFAEE
                     DGSIANSGRWLQWHWKAADAPHEAKPDVDILSEIRAELIHLYHKEGGKAPLEPLEAMT
                     WNYMNPLEPKPEEIAKENNGYALEDLKDADGNIIVKKGELLSSFTQMRDDGTTSGGCW
                     IYCGQWTPKGNQMANRDNSDPSGLGNTLGWAFSWPVNRRILYNRASADLSGKPWNPKR
                     QLVKWNGKNWNYVDVADYGTAPPNSPVTPFIMQPEGVSGLFVRERMQDGPFPEHYEPF
                     ETPIGTNPLHPNVISSPVARILPSDKEDLGTSDEFPYVGTTYRLTEHFHYWTKNALIN
                     VIAQPEQFVEIGEKLAEEKGIKNGDKVRVWSKRGEIKAVAVVTKRIRSLVSDGKPIHT
                     IGVPIHWGFAATTGAKKGYFANNLTVRTGEPNSNTPESKCMLLNIEKIVGA"
     gene            3075..3989
                     /gene="fdxH"
                     /locus_tag="PXB27_001930"
     CDS             3075..3989
                     /gene="fdxH"
                     /locus_tag="PXB27_001930"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HDL5346689.1"
                     /translation="MSTVQTQNIIKISATSALTPAPQARSHQVEVAKLIDVTTCIGCK
                     ACQVGCSEWNDIRSDVNANCVGVYDNPADLNAKAWTVMRFNEVEENDRLEWLIRKDGC
                     MHCAEPGCLKSCPSPGAIIQYANGIVDFQSDKCIGCGYCIAGCPFNIPRMNPDDNRVY
                     KCTLCVDRVNVGQEPACVKTCPTGAIRFGSKEEMKFYAEQRIADLKSRGYENAGLYDP
                     EGVGGTHVMYVLHHADKPELYSGLPKDPKIDTTVTLWKDVLKPVAAVALGGLALAEVA
                     HYITVGPNVEEDLEEHHHSENAEGGKHE"
     gene            3982..4659
                     /locus_tag="PXB27_001931"
     CDS             3982..4659
                     /locus_tag="PXB27_001931"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HDL5346690.1"
                     /translation="MSKKFEITNDTKIVRHKPLARVSHWFLVISFFMTMFTGVAFFFP
                     DFAWLTEILGTPQIARAVHPFTGIIMFIAFIIMALIYWHHNIPEKNDIRWAKGIVEVL
                     KGNEHKVSDNGKYNLGQKLLFWTLILAMFTLLATGIIMWRQFFSHYFSIPMLRIAILL
                     HSASAFMLFTGILVHMYMAFWVKGSIRGMVEGWVTVRWAKKHHPRWYREEVLPELERQ
                     VAEQNNK"
     gene            4715..5632
                     /gene="fdhE"
                     /locus_tag="PXB27_001932"
     CDS             4715..5632
                     /gene="fdhE"
                     /locus_tag="PXB27_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005622482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HDL5346691.1"
                     /translation="MSIKILPQDDIKQAASSFQQPELLFANPKNLYLRRAKRLRELAK
                     QNPFGEYMEFSANLVDIQLALLESQPIADYSQKITACVERTQGETPLNAQNFQRTDEW
                     RTLLLAIIEKFKPYANDTVLATIEVLEKAAKSELDTLADNLLNERYELVGADKAVFIW
                     AALSLYWVQLAQQLPRNSRAEIGHKHLCPVCNSAPVSSVIHFGEAQGLRYLHCSLCES
                     EWHVVRTKCTNCDETGKLDYWSLDTVEAAVKAESCGDCHSYLKVLYQDKDVNVEPVAD
                     DLATLFLDSEMEQKGLSRSGINPFLFQVE"
     gene            complement(5702..6088)
                     /locus_tag="PXB27_001933"
     CDS             complement(5702..6088)
                     /locus_tag="PXB27_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346692.1"
                     /translation="MLRNFMIMLHFIYGFAMKNAFSFFVISLFLTMNSISYASNPIPS
                     NKEKLNGVKKQQDKPKFRISERKVLKNKEKDEEVSNFSMEELRVGAQRPLDDSVIQYF
                     KSQCRYAFMSDREIIANRCEVKKIAD"
     gene            complement(6099..7469)
                     /gene="gorA"
                     /locus_tag="PXB27_001934"
     CDS             complement(6099..7469)
                     /gene="gorA"
                     /locus_tag="PXB27_001934"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009499791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HDL5346693.1"
                     /translation="MAKHYDYIAIGGGSGGIASINRAASYGQKCAIIEAKHLGGTCVN
                     VGCVPKKVMFYGAQVAEAINHYAPDYGFDITVNNFDYAKLVESRQAYIGRIHTSYGNV
                     LAKNNVDVLNGFARFKDAKTLEVSYADGSVEEVTADHILIATGGRPTVPNVKGAEYGI
                     TSDGVFALDALPKSVAIVGAGYIAVELAGVFNSLGVNTHLFVRQHAPMRNQDPLIVDT
                     LLEVLAQDGIELHTQATPQEVVKNADGSLTLKLQDGRETSVESLVWAIGREPATDKIN
                     LEAAGVETNERGFIKVDKFQNTNVPNIYAVGDIIEGGIELTPVAVAAGRRLSERLFNN
                     KPNEHLDYSLVPTVVFSHPPIGTVGLSEPKAIEQYGAENVKVYKSSFTAMYTAVTQHR
                     QPCRMKLVCVGEGEKVVGLHGIGFGVDEMIQGFAVAIKMGATKADFDNTVAIHPTGSE
                     EFVTMR"
     gene            complement(7546..>7907)
                     /locus_tag="PXB27_001935"
     CDS             complement(7546..>7907)
                     /locus_tag="PXB27_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HDL5346694.1"
                     /translation="DSNTVMLAVLNLIDVVMIANLLVMVIVGGYETFVSRLKVDDHPD
                     QPEWLDHVNATILKVKLSMAIISISSIHLLQTFINASNLDEKQMMWQVVIHLAFIVSA
                     LAMAYIDKMLNSGHMKH"
BASE COUNT         2384 a         1737 c         1647 g         2139 t
ORIGIN      
        1 atgcagatct caagacgtaa gttcttcaag gtctgtgcag gtggtatggc gggaacttct
       61 gctgcaatgt tgggttttgc tccagcaaca gcattagctg caccgcgtga gtataagctc
      121 ttaagagcaa aagaaactcg acaaacctgt acttactgcg cggtaggctg tggcatgatt
      181 atgtacagct taggcggcga agcaatgaac agcaaaggaa agttaattca tattgagggt
      241 gattctgacc atccagtaag ccgtggggcg ttatgtccga aaggtgcagg ggcgttggac
      301 tatgttaata gtgaccgccg tgtgcattat ccggaagtgc gtgaagccgg ttcaagtgag
      361 tggaaacgca tttcttggca cgaggcaatt gaacgtattg cccgccatat gaaaaatgac
      421 cgtgatgaga acttcgtcac ccacaatgaa gcagggctgg aagtaaaccg ttggatgacg
      481 gcaggtttct tggcaggctc ggcgtgtagt aacgaaaccg gtatcgtcac ccagaagttt
      541 atccgctctc tcggtatgat ttttaccgac aaccaagcga gtatctgaca cggaccaacg
      601 gtagcaagtc ttgctccatc atttggtcgc ggtgcgatga ccaaccactg ggttgatatt
      661 aaaaactccg atttagtgat cgttatgggc ggtaacgcag ccgaagctca ccctgtgggt
      721 ttccgttggg cagttgaagc gaaaaaacag aacggtgcga agttaatggt ggtagatcca
      781 cgctttaacc gtagtgctgc ggtggcggat atttatatac cattgcgtcc gggtacggac
      841 attgcgttct tgttaggtgc ggtaaactac ctgcttaaaa acgacaaaat tcaacacgag
      901 tatgtgaagc attacaccaa tgccacattc ttactcaacg aagggtttaa attcgaagac
      961 gggcttttca ccggctacga tgaagccact cgtacctata aagacaaatc cacttgggct
     1021 tatcagttag atgaacaggg tcagccgaaa cgggatatga caatgcaaga tccacgttgt
     1081 gtgatcaata tgctcaaaga ccacgtttcc cgctatacgc ctgaaatggt agaacgcatt
     1141 accggtacgc cacaaaaaga tttcctgatt ttctgtgaag agattgcgaa aacctcagcg
     1201 ccggataaag ccgccacctt cctctatgcc ttaggttgga cacaacacac cgtgggcgca
     1261 caaaacattc gctcaatggc gatcattcag ttgctgtgcg gtaacattgg tgtctccggc
     1321 ggtggggtaa acgcactgcg tggtcactct aatgtacaag ggattaccga cctaggcttg
     1381 ttcccacata tgttgccggg ctatatccgc ctgccaaccg aagcggacgc aaccttagaa
     1441 agctacttaa atgcgattgc accgaaaacc ttagtggcag ggcaagtaaa ctacgggcaa
     1501 aacaacccga aattcgctgt gagtatgctc aaaaccttct acggcgataa ggcaaccaaa
     1561 gagaacgagt tcggctatca ctatttaccg aaatacccga aaggcggtac agatcacttc
     1621 cgctttatcg aacagatgta tcaaggcaaa gtgaacggct atttctgcca aggtatgaac
     1681 ccgattgcct cttaccctaa ctcgcagaag ttaattaaag cgttgagcaa tttgaaatat
     1741 ttagtcattt ttgacccgct tattaccgat acttccgagt tctggaaaaa tcacggtgag
     1801 ttcaacaatg tgaacccgtc tgaaattcaa accgaagtgt tccgcttgcc gaccacttgc
     1861 tttgcggaag aagatggttc aattgcgaac tccggtcgct ggttacaatg gcactggaaa
     1921 gcagcagacg caccacacga ggcaaaaccg gacgtggata ttctgtctga aatccgtgcc
     1981 gaactgatcc acctttatca taaagaaggc ggaaaagcac cgcttgaacc gttagaagcg
     2041 atgacgtgga actatatgaa cccgttagaa ccgaagccgg aagagatcgc caaagagaac
     2101 aacggttatg cattggaaga tctgaaagat gcagacggca acatcatcgt gaaaaaaggc
     2161 gaactattat ccagctttac acaaatgcgt gatgacggta caacctccgg tggttgttgg
     2221 atctactgcg gtcaatggac tccgaaaggt aaccaaatgg cgaaccgtga taactccgac
     2281 ccatcaggct taggtaacac cttaggctgg gcattctcgt ggccggtaaa ccgccgtatt
     2341 ctatacaacc gtgcatcggc agatttaagc ggtaaaccgt ggaacccgaa acgccagcta
     2401 gttaaatgga acggtaaaaa ctggaactat gtggacgttg ccgattacgg tacagcacca
     2461 ccaaatagcc ctgtgacacc atttattatg caaccggaag gggtatccgg cttgttcgtg
     2521 cgtgaacgta tgcaagacgg tccattccct gagcattatg aaccgtttga aacgccaatc
     2581 ggcacgaacc cgttacaccc gaatgtaatc agcagtccgg tggcacgcat tctgccgagc
     2641 gataaagagg atttaggcac cagcgatgag ttcccatatg tcggtacaac ttaccgctta
     2701 accgaacact tccactactg gacgaaaaat gccctgatta acgtcattgc tcaaccggag
     2761 caattcgtgg aaatcggcga gaaacttgcc gaagagaaag gcattaaaaa tggcgacaaa
     2821 gtacgtgttt ggtctaaacg tggcgaaatt aaagccgttg ccgtggtgac taaacgtatc
     2881 cgttcattgg tctctgacgg caaaccaatc cacactatcg gtgtgccgat ccactggggc
     2941 tttgcggcta caaccggtgc gaaaaaaggc tattttgcca ataacttaac cgtgcgaaca
     3001 ggtgaaccaa actcaaatac acctgaatcg aaatgtatgt tattaaacat tgagaaaatt
     3061 gtgggggctt aacaatgagt acagttcaaa ctcaaaacat tattaaaatc tccgcaacct
     3121 cggcgctgac accggcgccg caggctcgtt cgcaccaagt ggaagtagcg aaactgatcg
     3181 atgtcaccac ttgtatcggc tgtaaagcct gtcaagtggg ctgttcagaa tggaatgaca
     3241 ttcgctccga tgtaaatgca aactgtgtcg gcgtgtacga caaccctgcg gatctaaatg
     3301 cgaaagcgtg gacagtaatg cgctttaacg aagtagaaga aaacgaccgt ttagaatggt
     3361 tgatccgtaa agacggctgt atgcactgtg ccgagccggg ttgcttgaaa tcttgcccgt
     3421 caccgggtgc gattatccaa tacgccaacg gtattgttga tttccaatcc gacaaatgta
     3481 tcggttgtgg ctactgtatc gcaggttgtc cgttcaacat tcctcgtatg aatccggacg
     3541 ataaccgtgt ttacaaatgc accctgtgtg tggatcgtgt caatgttggt caagaaccgg
     3601 cttgcgtgaa aacctgcccg accggtgcaa tccgcttcgg tagcaaagaa gagatgaagt
     3661 tctatgcaga acaacgcatt gctgatctga aatctcgtgg ctatgaaaat gccggcttgt
     3721 acgaccctga aggcgttggc ggtacgcacg taatgtatgt attacatcat gccgacaaac
     3781 cggagcttta ttcaggctta ccgaaagatc cgaagattga taccacagta accttatgga
     3841 aagacgtttt aaaacctgtg gcggcagtag cgttgggtgg tttagcttta gcagaagttg
     3901 cacactatat taccgttggg ccaaacgttg aggaagatct tgaagaacac caccatagcg
     3961 aaaatgcaga aggaggcaaa catgagtaag aaatttgaaa tcacgaacga taccaaaatc
     4021 gttcgtcata agcccttagc tcgtgttagc cactggttct tagtaatctc tttttttatg
     4081 acgatgttta ccggtgttgc cttcttcttc cctgactttg cttggcttac cgagatttta
     4141 ggtactccac agattgcccg tgcagtccac ccatttaccg ggattataat gtttattgca
     4201 tttattatta tggcgttaat atattggcac cataatatcc ctgagaaaaa tgatattcgt
     4261 tgggcaaaag gcattgttga agtactaaaa ggtaatgagc ataaggtttc cgataatgga
     4321 aaatacaatt tgggtcaaaa attactcttt tggactctta ttcttgcgat gttcactctt
     4381 ttagccacag ggattattat gtggagacaa tttttctctc actacttctc gattcctatg
     4441 ctaagaattg caattttact tcattcagca agtgcattta tgctatttac cggaatttta
     4501 gtacatatgt atatggcatt ctgggtaaaa ggctcaatca gaggtatggt tgagggttgg
     4561 gttaccgttc gctgggcaaa aaaacaccac ccaagatggt accgtgaaga agtgctacct
     4621 gaattagaaa gacaagtagc tgagcaaaat aataaataac ctcaagtgcg gtattattac
     4681 cgcactttta ttttattggt ttaaaaagaa tattatgagt atcaaaatac tacctcaaga
     4741 tgatatcaaa caagcagcta gttcatttca gcaacccgag ctcttatttg ctaatcccaa
     4801 aaatctgtat ttacgccgcg ctaagcggct acgtgaatta gccaagcaaa acccattcgg
     4861 tgaatatatg gagttttctg ctaacctcgt ggacattcaa ttagcattac ttgaaagcca
     4921 gccaattgca gattattctc aaaaaataac cgcttgtgta gagcgcacgc aaggagaaac
     4981 accgctaaat gcacagaatt ttcagcgtac tgatgaatgg cggacactac tcttagccat
     5041 tattgagaaa ttcaagcctt atgcaaatga tactgttctt gcaacaattg aagtattgga
     5101 aaaggcagct aagtcagagc tagacacact tgctgataat ttgctaaatg aacgatacga
     5161 acttgtgggc gcagataaag ctgtctttat ttgggctgct ctctcacttt actgggtaca
     5221 attagctcaa caattaccac gtaattctcg tgcagaaata ggccataaac atttatgtcc
     5281 ggtatgtaac tcagcacctg taagcagtgt gattcatttc ggtgaagctc aagggttacg
     5341 ttatctacac tgttctttat gtgaaagtga atggcatgta gtacgtacaa agtgtacaaa
     5401 ctgtgatgaa accggcaaac tggattattg gagcttagat acagtagaag cagcagttaa
     5461 agctgaaagt tgtggcgact gccattccta cttaaaagta ctctatcaag ataaagacgt
     5521 gaatgtggaa cctgttgctg atgatttagc aactctattc ttagattccg agatggaaca
     5581 aaaaggtctt tcaagaagtg ggataaatcc atttttattt caagtagagt aactcattta
     5641 caacaaaaac ggagcataac gctccgtttt ttattacatt taactcatac actatacttt
     5701 tttaatcagc tattttttta acttcacagc ggttagcaat aatctcccga tctgacataa
     5761 atgcgtatcg gcattgagat ttaaaatatt gaatcacaga atcatcaaga ggtcgttgtg
     5821 ctccaacacg taattcctcc atagaaaaat tactaacctc ttcatctttc tctttatttt
     5881 tcaatacctt acgttcagat attctaaact taggcttgtc ttgctgtttt tttactccgt
     5941 ttaatttttc tttattacta ggaataggat tacttgcata actaatacta ttcatcgtaa
     6001 gaaaaagaga aataacaaaa aagctaaaag cgtttttcat agcaaaccca taaataaaat
     6061 gtagcataat cataaaatta cgtaacattt tattacattt agcgcatggt tacaaattct
     6121 tcagatcctg tcgggtgaat agccactgtg ttgtcaaaat cagctttcgt tgcgcccatt
     6181 ttaattgcaa cagcaaaacc ttgaatcatt tcatcaacac caaaaccaat accatgtaac
     6241 ccaaccactt tttccccttc cccaacacaa actaatttca ttcggcaagg ttgacgatgt
     6301 tgtgtaaccg cagtatacat tgcagtaaat gaagatttat acactttcac attttctgcc
     6361 ccatattgct caatcgcttt tggctcactt aaaccaacag tacctattgg tgggtggcta
     6421 aaaacgactg tcggtactaa gctataatcc aaatgttcat ttggtttatt attgaacaac
     6481 cgttccgata gcctgcgacc agctgcaact gcaacaggag ttaattcaat accaccttca
     6541 ataatatcac caacagcata gatatttggc acattagtat tttggaattt atccactttg
     6601 ataaaaccac gctcgttggt ttcaacacca gcagcctcta aattaatctt atctgtcgct
     6661 ggctcacgac caatagccca caccaagctt tcaacagagg tttcacgacc atcttgcaat
     6721 tttaatgtca atgaaccatc tgcattttta actacttctt gaggagtcgc ttgtgtatgt
     6781 aattcgatcc catcttgagc taatacttca agcaatgtat ccactattaa tgggtcttga
     6841 ttacgcattg gagcatgttg acgaacaaaa agatgggtat ttactcctaa actattaaat
     6901 actccagcta attcaactgc aatataccct gcccccacaa ttgctacgga cttcggtaaa
     6961 gcatctagtg caaatacacc atcagaagtg ataccgtatt cagccccttt cacattgggt
     7021 acagttggac gaccacctgt tgcaatcaga atatggtctg ctgtgacctc ctcaacggaa
     7081 ccatcagcat aactcacttc taatgtttta gcatctttaa agcgagcaaa accatttaaa
     7141 acatcaacat tattttttgc cagcacatta ccatatgagg tatgaatgcg cccaatatag
     7201 gcctggcgac tctcaactaa tttggcataa tcaaaattat ttaccgtaat gtcaaaacca
     7261 taatcagggg cgtaatggtt aatcgcttct gcaacttgtg caccatagaa catcactttt
     7321 ttgggtacac atcccacgtt cacacaagtg ccacctaaat gttttgcttc aataatggca
     7381 catttttgcc catagcttgc tgctcgatta attgaagcaa taccaccact accaccgccg
     7441 atagcaatat aatcataatg tttagccata agctattcct cgatctataa aaacaaaagc
     7501 aacttattcg ttaagcgctt tatcaaatta aatttgcata gaagactaat gtttcatatg
     7561 ccctgaattc aacattttat caatatacgc catcgctaat gctgaaacaa taaaggctaa
     7621 gtgaataact acttgccaca tcatttgctt ttcatcaaga tttgaagcat tgataaatgt
     7681 ttgtaataaa tgaatagaag aaatactgat aattgccatt gaaagtttta ctttcaaaat
     7741 agtcgcatta acgtgatcca accattctgg ctgatcaggg tgatcatcta cttttaatct
     7801 agagacaaaa gtttcatatc ctccaacaat aaccataacc aaaagatttg caatcatcac
     7861 cacatctatt aaatttaata ccgctaacat aacagtattt gaatcca
//
LOCUS       DANLUQ010000118         7877 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.119, whole genome shotgun sequence.
ACCESSION   DANLUQ010000118 DANLUQ010000000
VERSION     DANLUQ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7877
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.119"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            42..977
                     /gene="pheS"
                     /locus_tag="PXB27_001936"
     CDS             42..977
                     /gene="pheS"
                     /locus_tag="PXB27_001936"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDL5346695.1"
                     /translation="MDQNLEALEEFRVEYFGKKGHFTTLMQELRNIAAEERPAIGAKI
                     NEAKQVVLDVLNAKKEEWEQAVLNAKLANESIDVSLPGRKTELGGLHPVSITIERVVK
                     FFSELGFTVANGPEIETDYYNFDALNIPDHHPARADHDTFWFDANRLLRTQTSGVQIR
                     TMENVKPPIRIVAPGRVYRNDYDQTHTPMFHQIELLYVDKKANFTELKGLIHDFLKAF
                     FEEDLQVRFRPSFFPFTEPSAEVDVMRQNGKWLEVLGCGMVHPNVLRNVGIDPEEYSG
                     FAVGMGVERLTMLRYNVTDLRSFFENDLRFLKQFK"
     gene            1025..3412
                     /gene="pheT"
                     /locus_tag="PXB27_001937"
     CDS             1025..3412
                     /gene="pheT"
                     /locus_tag="PXB27_001937"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDL5346696.1"
                     /translation="MKFNESWLREWVNPAISTEQLCDQITMLGLEVDDVEPVAGAFSG
                     VVVGEVVECAQHPDADKLRVTKVNVGGERLLDIVCGAPNCRQGLKVACAVEGAVLPGD
                     FKIKKTKLRGQPSEGMLCSYSELGIKEDHSGIIELPADAPIGQDFREYLNLNDVAIEI
                     SLTPNRADCLSIAGIAREVGVINRVDVKAPTITDVKATISDKVSVELQAPEACPRYLA
                     RVVKNVNVKATSPLWLQEKLRRCGIRSIDPIVDITNLSLLELGQPMHAFDAAKIEGGI
                     QVRMAKEGEELVLLDGTNAKLQSNTLVIADSKGALAMAGIFGGEASGVSENTKDVVLE
                     SAFFAPLAITGRARQYGLHTDASHRFERGVDPELTRQAMERATALLLEICGGEAGEIV
                     EAVSQENLPKRNTVTLRRSKLDSVIGHHIDDATVTDILTRLGLNVTFADNSWTAIAPS
                     WRFDIEIEEDLIEEIARIYGYNSIPNNSPLAHLTMKGTPEKLLEANRIRMALVDSDYQ
                     EVITYSFVDPKKQSLLHPQQEALILPNPISSEMSAMRVSLLTGLLDTIVYNQSRQQNR
                     VRIFEAGLRFIPDSNAESGVRQEFVFGAAIVGDKRPVHWENKSEAADFFDLKGDIERI
                     LSLTSIRHDLQFVAKQFPALHPGQSAAIMLDGKEIGFIGTVHPSIVQKFGIKGKPIVC
                     EILASAIAERPVTQAGEISKFPANNRDIAVVVDESVPAGEVLEACRKAGGNKLVAVNL
                     FDVYRGSNLESGKKSLAISLTVQDNEKTLEENEINAVIQAVLAELATRFNAVLRD"
     gene            3470..3766
                     /locus_tag="PXB27_001938"
     CDS             3470..3766
                     /locus_tag="PXB27_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005710633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HDL5346697.1"
                     /translation="MALTKIEIAENLIEKFGLEKRVAKQFVELFFEEIRSSLENGEEV
                     KLSGFGNFSLREKKARPGRNPKTGENVAVSARRVVVFKAGQKLRERVEQASSQS"
     gene            3805..4326
                     /locus_tag="PXB27_001939"
     CDS             3805..4326
                     /locus_tag="PXB27_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL5346698.1"
                     /translation="MKLTKRWLSLGCLLAITAMLVACSSSDTTSKSQVKSKVYRSIYQ
                     SDLSDPIMAISRLSEHQHEWKGTRYRLGGNSKAGIDCSGFMQVTFKDLFGIDLPRTTT
                     EQAKAGERISKDELRTGDLVFFKTGRGPNGKHVGVYVKNGQFLHASTKGGVIYSDLDS
                     PYWSKAFWQARRL"
     gene            4342..5385
                     /gene="degS"
                     /locus_tag="PXB27_001940"
     CDS             4342..5385
                     /gene="degS"
                     /locus_tag="PXB27_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HDL5346699.1"
                     /translation="MLKKIGQAVIFGLFCAILILFVAPIIQSKGSSFSLSAFTQKEIV
                     SYNAAVKIASPAVVNVYSRNFSSENETFEVKNLGSGVIMSSDGYILTNRHVIEDADQI
                     VIALQNGAISTNVKLVGDDSLTDLAVLKIEGENLPTIPQNPERKLQIGDVVLAIGNPL
                     NLGQSITQGIVSAIGRKTLTESGRQNFIQTDVSINQGNSGGALINTAGELIGINTLRF
                     GKNTNELSEGLNFAIPINLANQIMSKIIKDGRVIRGYFGVSTDLLDTAKMQGYEKGVI
                     IVNVDKNGPAAKAGIQAGDLVLKVGDIEAFSPSQMMEELAEMKPYTTVKVLVQRDEQL
                     LNFDVTITELQTQ"
     gene            5394..6068
                     /gene="cmk"
                     /locus_tag="PXB27_001941"
     CDS             5394..6068
                     /gene="cmk"
                     /locus_tag="PXB27_001941"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDL5346700.1"
                     /translation="MKKIVITVDGPSGAGKGTLCHALAEKLGFDFLDSGALYRITALS
                     AIKKEVPLDDEEKLAEVGRNLDIQFLPENGEIQVILEGENVGDLIRTAEAGQNASKVA
                     AFPLVREALLERQRAFSSEKGLIADGRDMGTVVFPDAQIKLFLDASAEERTKRRVKQL
                     QEKGFNANFDEILAEIKERDFRDRNRSVAPLVPAKDALLLDSTHLSIEDVINQALNHI
                     AQYIKF"
     gene            6160..7827
                     /gene="rpsA"
                     /locus_tag="PXB27_001942"
     CDS             6160..7827
                     /gene="rpsA"
                     /locus_tag="PXB27_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDL5346701.1"
                     /translation="MSELSFAQLLEESFAATPRLGDVVKGTVVAIQKGLVIVDTGFKS
                     ESAIDASEFTNAQGELEVQVGDEVDVVLKAVEDGFGATVVSRGDAKRNEAWIALEKAF
                     EEQATVIGFVNGKVKGGFTVELNGVRAFLPGSLVDTRPVRDSLNLEGKEYEFKVIKLD
                     QKRNNVVVSRRAVIETESNQDRDAVLANLVEGSEVKGTVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEVVNVGDEVTVKVLKFDKDKTRVSLGLKQLGQDPWAVIAENHPV
                     NSKLTGKVTNLTDYGCFVEILDGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLE
                     VDEERRRISLGLKQCKANPWEQFAATHSKNDKVSGKIKSITDFGIFIGLEGGIDGLVH
                     LSDISWNVSGEEAVRNYKKGDEVEAVVLQVDAVKERISLGIKQLESDPFTNFVDGTKK
                     GAILTGKVVEVDAKGVKVELEGGVEAFIRANEATAERVEDITSVISVGDSIEAKYTGV
                     DRKSRVVNLSVRAKNEAEESAAIAQVNKEEVVVPNAFAEAFKAAKGE"
BASE COUNT         2464 a         1288 c         1837 g         2288 t
ORIGIN      
        1 taaaagattt aactaataaa gcacgtgaag ctttagataa gattgatcaa aatttagagg
       61 ctttagaaga gtttagagtc gaatattttg gtaaaaaagg gcattttaca acgttaatgc
      121 aagaattacg caatattgca gcggaagaaa gaccggcaat cggagcaaaa attaatgaag
      181 caaagcaagt tgtgcttgat gtgttaaatg ccaaaaaaga agaatgggaa caagctgtat
      241 taaatgcaaa attagctaat gagagcattg atgtttcact gccggggcgt aaaactgaat
      301 taggtggttt gcaccctgtt tctatcacta ttgagcgtgt ggtgaaattt ttctctgaat
      361 taggttttac cgtagcaaac ggacctgaaa ttgaaaccga ttactataac ttcgatgcgt
      421 taaacattcc ggatcatcat ccggctcgag cggatcacga tacgttttgg tttgatgcca
      481 atcgtttatt aagaacccaa acctcgggtg tgcaaatccg tacaatggaa aatgtaaaac
      541 cgcctatccg tattgtggca ccggggcgtg tatatcgtaa tgactacgac caaacccaca
      601 caccgatgtt tcaccaaatc gaattgttat atgtggataa aaaagcaaac tttaccgagt
      661 taaaaggctt aattcacgat tttctcaaag cattctttga agaagattta caagtgcgtt
      721 tccgcccgtc attcttccca ttcactgagc cttctgcaga agtagatgta atgcgtcaaa
      781 acggcaaatg gttagaagtg ttaggttgcg gtatggtaca cccgaatgtg ttacgcaatg
      841 tagggattga ccctgaagaa tatagcggtt ttgctgtggg aatgggcgtc gagcgtttaa
      901 cgatgttacg ctacaatgtc acggatttac gttcattctt tgaaaatgac ttacgtttct
      961 taaaacaatt taagtaatac aagcggttaa aaatttagaa aattttacaa ataggataga
     1021 aataatgaaa ttcaatgaat cttggttgcg tgaatgggta aacccagcaa ttagcacaga
     1081 acagttatgc gaccaaatta caatgttagg gttagaagtt gacgatgtag agccggtagc
     1141 tggtgcgttt tcgggtgtag ttgtgggtga agtggtggaa tgcgcccagc acccggatgc
     1201 tgacaaattg cgtgtcacta aagtgaatgt tggtggcgaa cgtttattag atattgtttg
     1261 tggtgcacca aactgccgtc aaggtttaaa agtggcttgc gctgttgaag gtgcagtgct
     1321 accgggcgat ttcaaaatta agaaaaccaa attgcgtggt cagccttcag aggggatgtt
     1381 atgttcttac tcagaacttg ggattaaaga agatcatagc ggtattattg aattacctgc
     1441 tgatgcacct attggtcaag atttccgtga atatttaaat ttaaatgatg tggcgattga
     1501 aatcagcctt acgccaaacc gtgccgactg cttaagcatt gccggcattg ctcgtgaagt
     1561 gggggtgatt aaccgtgtcg atgtgaaagc tccaactatt acagatgtaa aagccacgat
     1621 ttcagataaa gtttcagtgg aattacaagc accggaggct tgccctcgct atttagcccg
     1681 tgtggtgaaa aatgtgaatg tcaaagcgac atcgccactt tggttacaag aaaaattacg
     1741 ccgttgtggg attcgctcaa tcgatccaat cgttgatatt actaacttga gccttttaga
     1801 attagggcag ccaatgcacg cctttgatgc agcgaaaatt gaaggtggca ttcaagtgcg
     1861 tatggcaaaa gaaggcgaag aattggtatt gctagatggc acaaacgcta aattacaatc
     1921 gaatacctta gtgattgctg atagcaaagg ggcattggct atggcaggta ttttcggtgg
     1981 cgaagccagc ggggtgagcg agaatacgaa agatgtggtg ttagagtctg cattttttgc
     2041 tccattagcg attaccggtc gcgctcgtca atatgggtta cataccgacg catcacaccg
     2101 ttttgagcgt ggggttgatc cagaattaac tcgtcaagca atggagcgtg caacggcatt
     2161 attactggaa atttgcggcg gtgaagcagg tgaaattgtg gaagccgtga gccaagaaaa
     2221 cctaccaaaa cgcaatactg tcacgcttag acgcagtaaa ttagattcgg tgattggtca
     2281 tcatattgac gatgcaacgg taaccgatat tttaacccgc ttaggcttga acgtaacatt
     2341 tgcggataat agttggacgg caattgctcc aagttggcgt tttgatattg aaattgaaga
     2401 agatttaatt gaagaaatcg cgcgaattta tggctacaac agtatcccaa ataattcgcc
     2461 acttgctcat ttaacgatga aaggtacgcc ggaaaaatta ctggaagcaa accgtattcg
     2521 tatggcattg gtagatagtg attaccaaga agtgattact tatagctttg ttgatccgaa
     2581 aaaacaatca ttattgcacc cacagcaaga agccttgatt ttaccaaacc cgatttcaag
     2641 tgaaatgtcg gcaatgcgtg tttctctttt aaccggtttg ttggacacta tcgtgtataa
     2701 ccaaagccgt cagcaaaacc gtgtgcgtat ttttgaagca ggtctacgtt ttattccgga
     2761 tagcaatgcg gaatcaggcg tgcgtcaaga atttgttttt ggtgcggcga ttgtaggcga
     2821 taaacgtccg gtacattggg aaaacaaatc agaagcagcc gatttctttg atttaaaagg
     2881 cgatattgag cgtattcttt ctttaacctc tatccgccac gatctgcaat ttgtcgcaaa
     2941 acagttccca gcattacacc cggggcaatc ggctgcgatt atgcttgatg gcaaagaaat
     3001 tggttttatc ggaactgttc acccgtcaat tgtgcaaaag tttggcatta aaggcaaacc
     3061 gattgtatgt gaaattctag catcagctat tgcagagcgt ccggttacac aagcgggcga
     3121 aatttctaaa tttcctgcaa ataaccgtga tattgcggta gtggttgatg aatctgttcc
     3181 tgcaggtgag gtgttagagg cttgtcgtaa agctggtggt aataaattag tggcagtaaa
     3241 tttatttgac gtttaccgtg gttcgaattt agaaagtggt aagaagagtt tagccattag
     3301 cttaacggtt caagataatg aaaaaacatt agaagaaaat gaaatcaatg cggtaattca
     3361 agcggtatta gctgaattag caacacgttt caatgcggta cttcgtgatt aatcagtaaa
     3421 atatggctga tttattttat cagccataac aataaactaa ggataaatga tggcgcttac
     3481 caagattgaa attgcagaaa acctgattga aaaatttggg ttagaaaagc gtgtagcgaa
     3541 gcaatttgtt gaattgttct ttgaagagat ccgatcctcc ctcgaaaatg gtgaagaagt
     3601 gaaattatct ggttttggta atttttcact gagagaaaaa aaagctcgtc caggaagaaa
     3661 tcctaaaacg ggggaaaatg tggctgtttc agcccgtcga gtagtagttt ttaaagctgg
     3721 gcaaaaatta cgagaacgtg ttgagcaagc tagctcccaa tcataggggc agttatatta
     3781 gttagattta atgtggagta ataaatgaaa ttaaccaaac ggtggctttc cttaggttgt
     3841 ttgttagcta ttacagcgat gttagtggct tgttcaagtt cagatacaac ctctaagtct
     3901 caagtaaaaa gtaaagttta tcgttcaata taccaatcag acctttctga tcctattatg
     3961 gcaattagtc gattaagtga acatcaacac gaatggaaag gcactcgcta tcgtttaggt
     4021 ggcaatagca aggctggcat cgactgttct ggttttatgc aagtcacttt taaagatctt
     4081 tttgggatag atttgccgag aaccaccaca gagcaggcta aggctggtga gcgtatatcc
     4141 aaagacgaat tgcgtacagg tgatttagtt tttttcaaga cagggcgtgg tccaaatggg
     4201 aaacacgttg gtgtttatgt aaaaaatggg caatttcttc acgcctcaac aaagggaggg
     4261 gtgatttatt ctgatttgga ttcgccatat tggtctaagg ctttctggca ggctcgtcgt
     4321 ttataattta gggtaacata catgctgaaa aaaatcggac aagcggtcat atttggactt
     4381 ttttgtgcaa ttttaatttt gtttgtagct ccaattattc agagtaaagg gagttcgttt
     4441 tcattatcag cattcactca aaaagagatt gtaagctaca atgcagcggt taaaatagcc
     4501 tcccccgcag tcgttaacgt atatagccga aattttagtt ctgaaaatga aacatttgaa
     4561 gttaagaatt taggttcggg tgtgattatg agtagcgatg gctatatttt aactaacagg
     4621 catgtgattg aagatgcgga tcaaatcgtg attgctttgc aaaacggtgc tatttcgact
     4681 aatgtaaaat tagtcggcga tgattcttta actgatttag ctgttttaaa aattgagggt
     4741 gagaatttac ctactattcc acaaaatccg gagcgcaaac tacaaattgg cgatgtggtg
     4801 ttggcaattg gtaatccttt gaatttagga cagagcatta cgcaagggat agtcagtgct
     4861 attgggcgaa aaaccttaac agaaagtggt agacaaaatt ttattcagac agatgtttcc
     4921 attaatcaag ggaactcagg tggtgcttta attaatactg ctggtgagct aataggtatt
     4981 aacactttgc gttttgggaa aaatacgaat gaattgtcag agggcctaaa ttttgcgatt
     5041 cccattaatc tggcgaatca aattatgagt aagatcatta aagatggccg agttattcgt
     5101 ggctattttg gggtaagtac agatttgctt gacaccgcaa aaatgcaagg ttatgaaaaa
     5161 ggggtgatca ttgttaatgt agataaaaat gggccagccg caaaagcggg gattcaagca
     5221 ggtgacttag tgcttaaagt cggagatatt gaggcattct ctccttctca aatgatggaa
     5281 gagcttgctg aaatgaaacc ttatactaca gtaaaagtat tggttcaacg tgatgaacaa
     5341 ttacttaact ttgatgtaac tattactgag ttacagactc aatagggtag aaaatgaaaa
     5401 aaatagtgat tacagttgat gggccgagtg gtgcaggtaa aggaacgtta tgtcatgcat
     5461 tggctgaaaa attagggttt gattttttag attctggagc cttataccgg attactgcat
     5521 taagcgcaat taaaaaagag gttccccttg atgatgagga aaaactagcg gaagttgggc
     5581 gtaatcttga tattcaattt ttgccagaaa atggcgagat tcaggtgatt ttggaaggag
     5641 aaaatgtagg cgacttaatc cgaacggcag aagcaggtca aaatgcctct aaagtcgctg
     5701 ctttcccttt ggtacgtgaa gcacttttag aacgccaaag agcctttagt agcgaaaaag
     5761 gtctgattgc agatggacga gatatgggga cagttgtgtt tcctgatgca caaattaagc
     5821 tgtttttaga tgcaagtgcc gaagaacgca caaaaagacg tgtaaaacag ttgcaagaaa
     5881 agggatttaa tgctaacttt gacgagattt tagccgagat aaaagaacgt gattttcgcg
     5941 atagaaatcg atcagtcgcg ccacttgttc cggctaaaga tgcgttgctg ttagattcaa
     6001 cgcatttatc aattgaagat gtgattaacc aagcattaaa tcatatcgct caatacatta
     6061 aattttagtt tgttgcttca cataggattt gtgtagcttt tattatcaac cccgtttaac
     6121 aggacgttag atggatgttt atttttaaga agatttatta tgtcagaatt atcgtttgct
     6181 caactacttg aagaatcatt tgcagctaca ccacgtttag gtgatgttgt taaaggtact
     6241 gttgttgcta tccaaaaagg cttagttatt gttgatacag gtttcaaatc tgaatctgca
     6301 atcgatgcct cagaatttac taacgcacaa ggtgaattag aagttcaagt gggtgatgaa
     6361 gtagatgttg ttcttaaagc tgtagaagac ggttttggtg caacagtagt ttctcgtggt
     6421 gatgcaaaac gtaacgaagc ttggattgct ttagagaaag catttgaaga acaagctaca
     6481 gttatcggtt tcgtaaatgg taaagtaaaa ggcggtttca ctgttgaatt aaacggtgtt
     6541 cgtgcattct tacctggttc attagttgat actcgtccgg ttcgtgattc tttaaaccta
     6601 gaaggtaaag agtacgaatt caaagtaatc aaattagatc aaaaacgtaa caacgtagta
     6661 gtatctcgtc gtgctgttat cgaaacagaa agcaaccaag atcgtgatgc ggtattagca
     6721 aacttagttg aaggttctga agttaaaggt acagttaaaa acttaactga ctacggtgca
     6781 ttcgtagatt taggtggcgt tgacggttta ttacatatca cagatatggc ttggaaacgt
     6841 gttaaacacc caagtgaagt ggttaatgta ggcgatgaag ttactgttaa agtattaaaa
     6901 tttgacaaag acaaaactcg tgtatcatta ggcttaaaac aattaggtca agatccttgg
     6961 gctgtaattg ctgaaaacca cccggtaaac agcaaattaa ctggtaaagt aactaactta
     7021 actgactacg gttgcttcgt tgagatttta gacggtgttg aaggtttagt tcacgtttca
     7081 gaaatggatt ggacaaacaa aaacattcac ccatctaaag tggttaacgt aggcgatgta
     7141 gttgaagtaa tggtacttga agttgatgaa gaacgtcgtc gtatctcatt aggtttaaaa
     7201 caatgcaaag caaacccttg ggaacaattt gcggcaactc acagcaaaaa tgataaagta
     7261 tcaggtaaaa tcaaatcaat tactgacttc ggtattttca tcggtttaga aggtggtatt
     7321 gacggtttag ttcacttatc tgacatttct tggaatgttt caggtgaaga agcagttcgt
     7381 aactacaaaa aaggtgatga agtagaagct gttgttctac aagttgatgc agtgaaagag
     7441 cgtatctctt taggtatcaa acaattagaa tctgatcctt tcacaaactt tgtagatggt
     7501 actaaaaaag gcgcaatctt aacaggtaaa gttgtagaag ttgatgctaa aggcgttaaa
     7561 gttgaattgg aaggtggcgt tgaagcattt attcgtgcaa acgaagcaac tgctgagcgt
     7621 gtagaagaca tcacttctgt tatttctgtt ggcgattcaa tcgaagcgaa atacaccggt
     7681 gttgatcgta aatctcgcgt agttaactta tctgttcgtg caaaaaacga agctgaagaa
     7741 tcagcagcaa ttgcacaagt gaacaaagag gaagttgttg tacctaatgc atttgctgaa
     7801 gctttcaaag cagctaaagg cgaataatta gtatagacta atctaaaatg ggtatgtttt
     7861 gaacataccc atttaaa
//
LOCUS       DANLUQ010000119         7709 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.120, whole genome shotgun sequence.
ACCESSION   DANLUQ010000119 DANLUQ010000000
VERSION     DANLUQ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7709
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.120"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            114..1022
                     /locus_tag="PXB27_001943"
     CDS             114..1022
                     /locus_tag="PXB27_001943"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDL5346702.1"
                     /translation="MNKNSLFSIWFTTARPKTLPLALASIIVGSALAHWAGKFDVVTT
                     LLAFITTILLQILSNFANDYGDHVKGSDTKERIGPLRAIQHGVIGGEQLKKAVIILSL
                     LSFVSGVALSAYAFETVQDLFFFIGLGIVSIIAAITYTVGKKAYGYLGLGDLFVLIFF
                     GFVAVIGVFYLQAHSLPIMILLPAFGCGLLSVAVLNINNLRDINQDRQAGKNTLIVRI
                     GSQNGRKYHVALLVLAVISYLIFAISEFEHWYSFIFLLSVPLLAKHARFVYQHKDPKE
                     LYPILGQMAGLALLTNLLFSLGIVLG"
     gene            complement(1098..2084)
                     /locus_tag="PXB27_001944"
     CDS             complement(1098..2084)
                     /locus_tag="PXB27_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDL5346703.1"
                     /translation="MKKSLLALLIGSVFLISACEDGKMTQTVLEAEKQIVQLGTELKN
                     AQADLAKKEAEVAELSGVKAENEKLKSELENAQQNTALNVEIVKIFDKKEVIKHQVDP
                     KQEFAIEESTVSAFVSLPKTNFDWLNQLLINQAYDYNEEQGRKLKNPTEEEFKKHREA
                     VYQGLVESVKTEPAIGYDDHISSAFIGQRNNIATFTMMHYTYMGGAHGMHYTQYINVD
                     LAKKAIIGLNDLISPKNQVQLKDILWENYSSVRVDENGNYNGFADKKEFRISDQFYFT
                     PHGIAFVYPPYELGAYAEGDIEVEADWYIINKLLNSEYQRGEKDGFFEELDN"
     gene            complement(2178..4049)
                     /gene="rpoD"
                     /locus_tag="PXB27_001945"
     CDS             complement(2178..4049)
                     /gene="rpoD"
                     /locus_tag="PXB27_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDL5346704.1"
                     /translation="MENQADQQLEQQSQLELLISQGREQGYLTLSEVHDHLPEELVDA
                     EQIEDIIQMINDMGIQVLEAAPDADDLLLAENIADEDVVEEATKVLSTVESELGRTTD
                     PVRMYMREMGTVELLTREGEIDIAKRIEEGINEVQCAIAEYPEALSGLLACYAQVETG
                     EMRLSDLVTAFVDPNILEEAPLEDIEAALGEDEESSDVPDVDDENEDENGDSSSDSSD
                     DSSIDPELAREKFEALRSQHEKVLLVIQKHGRTSKKSKEQIEILSHIFREFRLVPKQF
                     DLLVASMQKLMKQVRTEERQIQRYAVEYAGMKKADFLKAFQGHELSEAWIEKAINAKK
                     NAAPKLANYVDNIRVNIANLQNLEQTSGLTIAQIREIGGRISNGELKARRAKKEMVEA
                     NLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAIT
                     RSIADQARTIRIPVHMIETINKLNRISRQCLQEMGREATPEELAERMGMPEDKIRKVL
                     KIAKEPISMETPIGDDDDSHLGDFIQDESLELPLDSATAESLRMATNEVLEGLTPREA
                     KVLRMRFGIDMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSETLRSFLDD"
     gene            complement(4308..6056)
                     /gene="dnaG"
                     /locus_tag="PXB27_001946"
     CDS             complement(4308..6056)
                     /gene="dnaG"
                     /locus_tag="PXB27_001946"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDL5346705.1"
                     /translation="MKGTIPRSFIDDLIARTDIVEVINSRVKLKKAGRDYQACCPFHH
                     EKTPSFSVSQSKQFYHCFGCGVHGNVITFLMDYDKLEFPEAIEELAAMHGLEVPRENV
                     IQRDGKPQANFKTKRNLYELMEAVAKFYQQNLSHQIEPQSYLQQRGLSEEIIERFEIG
                     FAPNAFDAALRTFGQSQEDIQKLLDTGVLTKNDSGRIYDKFRNRVMLPIRDKRGRVIA
                     FGGRVLPNDEQGAKYMNSPETVIYHKGSELYGLYQALKVNETPDYLFVVEGYMDVVAL
                     AQFGVDNAVASLGTATTGEQIQQMFRCTEQIVCCYDGDRAGRDAAWRALENALPYLQD
                     GRQLKFIFLPDGEDPDTFVRSQGKEGFEQYVAQNAKPLSDFLFDSLLADVDLSSKEGK
                     SKLAALAVPLINRIPGEMLRVYLRNILGQKLGILDPTQIEALLPTKKAAEKPLHQAVK
                     MKRTPMRLLIALLIQNPYFVKFVPDVSALASLKDPGFTLLTDIVATCQQNAGISMGGL
                     LEHYREDPQYKIVEALANWDHLELPENIERTFIDTLDFLYAKLVELRKEELIAKDRTV
                     GLTDDEKKEYALLMFQ"
     gene            complement(6210..6425)
                     /gene="rpsU"
                     /locus_tag="PXB27_001947"
     CDS             complement(6210..6425)
                     /gene="rpsU"
                     /locus_tag="PXB27_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005701713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDL5346706.1"
                     /translation="MPVIKVRENESFDVALRRFKRSCEKAGILAEVRAREFYEKPTTI
                     RKREKASLAKRHAKRNFRENARNTRLY"
     gene            complement(6573..7169)
                     /locus_tag="PXB27_001948"
     CDS             complement(6573..7169)
                     /locus_tag="PXB27_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HDL5346707.1"
                     /translation="MSLLPMNEITSLNQLDLQKSYSYADYLLWKFKERVEIIKGKIVA
                     MSPAPTRFHQRISMKMTAKFLEVFDNHQCQIYSAPFDVRFPDENGKVKTVVQPDLCVI
                     CDVSKLDERGCVGAPDLVVEILSPGNTKREMKDKYELYQEQGVKEYWIVSPETRTIQI
                     FVLENGKYIGIQPVAEDEPATSVVFPALSFSTEKLFEL"
     gene            7311..>7709
                     /locus_tag="PXB27_001949"
     CDS             7311..>7709
                     /locus_tag="PXB27_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDL5346708.1"
                     /translation="MHLNFKKIDNSHIALFYNVLVATFFVTVLTFKKGYSYVPMALGI
                     IATLSFLFYRFKLKQKWLLDKEDKFFIFSLIGYFLTFAMSAIFNGDGIREIDNPIQIL
                     LLIPLIILFRLYPIKKETVLHFIPIGALVAG"
BASE COUNT         2110 a         1725 c         1412 g         2462 t
ORIGIN      
        1 agcgtccacg cttgtaccaa ttcgttttac catttaacac aagagtggac gcttgcacta
       61 tcgcttaaat ccttaacatc attgtaaatg aaattacgct aagattattc tctatgaaca
      121 aaaattctct cttttcgatt tggtttacca ccgctcgccc gaaaacttta ccgctggcgt
      181 tagcttcaat tattgtgggt tcagcacttg ctcattgggc agggaaattt gatgttgtta
      241 ccaccttact cgcatttatc accaccattt tgttgcagat cctgtctaac ttcgccaatg
      301 attacggcga ccacgtcaaa ggctctgaca ccaaagaacg aatcggaccg ctacgggcga
      361 ttcaacacgg tgttattggc ggtgaacaac ttaagaaagc cgtgattatc ttaagcctac
      421 tctcgtttgt ttccggtgtt gccttaagtg cctatgcttt tgaaacagtg caggatctgt
      481 ttttctttat cgggcttggc atcgtctcaa ttattgccgc cattacttac accgtcggca
      541 aaaaagccta cggctatttg gggctgggcg atctatttgt gctgattttc tttggctttg
      601 tagcggtaat cggcgtgttc tacttgcagg ctcatagcct acctataatg attctgctcc
      661 ctgctttcgg ttgcggattg ctgtctgtag cagtgttaaa tatcaataac ttgcgtgata
      721 ttaaccaaga ccgacaagct gggaaaaaca ctctcattgt acgaatcggt agccaaaacg
      781 ggcgaaaata ccacgttgca ttgttagtgc tagcggtcat ttcttatctg atttttgcaa
      841 tttctgaatt tgaacattgg tatagcttca tttttttgct gtctgtaccg ttactggcaa
      901 aacacgctcg ttttgtctat cagcataaag acccaaaaga actctaccca attctagggc
      961 aaatggcagg gctggcgttg ctcactaatc tattatttag tttgggcatt gtgttagggt
     1021 aagttgcaaa attttaacaa aactaaaccg cttgtaataa aggattacaa gcggttgttt
     1081 tttatctaaa tttacaatta gttatctagt tcttcaaaga atccatcttt ttcgccacgt
     1141 tgatactccg aattgagcaa cttattaata atgtaccaat ccgcttcaac ctctatatcg
     1201 ccttctgcat aagcacctaa ttcgtatggc ggataaacaa aagcaatgcc gtgaggggta
     1261 aagtagaatt ggtcagaaat acggaactct tttttatcag caaatccatt atagttgcca
     1321 ttttcatcca cacgcacgct actgtaattt tcccaaagaa tgtctttcag ttgaacttga
     1381 ttttttgggc tgattaaatc atttaagcca atgattgctt ttttagctaa atctacattg
     1441 atatattgcg tatagtgcat accgtgagcc ccacccatat aggtgtaatg catcatagtg
     1501 aatgtagcaa tattattgcg ttgcccgata aaagcactgg aaatatgatc atcatagcca
     1561 atagctggct cggttttcac actttctacc aacccttgat aaaccgcttc acgatgcttt
     1621 ttaaactctt cttcggttgg atttttcagc tttctgcctt gttcttcatt gtaatcatag
     1681 gcctgattaa tcaaaagctg gttaagccaa tcaaaattgg tttttggtag agacacaaac
     1741 gcacttactg tactttcttc tattgcaaat tcttgtttcg gatcaacttg gtgcttaatc
     1801 acctctttct tatcaaagat tttcacaatt tcaacattta acgctgtgtt ttgctgagcg
     1861 ttctctaact ctgattttaa tttttcattt tctgctttca cccctgaaag ttctgcaact
     1921 tcagcctctt tttttgctaa atcagcctga gcatttttta attctgtgcc taactgaaca
     1981 atttgttttt cggcttctaa taccgtttgg gtcattttgc cgtcttcaca agctgaaatc
     2041 agaaatactg agccaattaa aagtgctaat agtgattttt tcatctctcc ctcttttgta
     2101 aaaaatacgg taaatatcac cgcttgttat acagattaaa acaaaatccc ctataccatc
     2161 aggcataggg gaaatgatta gtcgtctaag aaactgcgta aggtttccga acggctcggg
     2221 tggcgaagtt tgcgcagtgc tttcgcctca atttggcgga tacgttcacg agttacgtca
     2281 aattgtttac caacttcttc gagtgtgtgg tctgtgttca tatcaatacc aaaacgcata
     2341 cgcaacactt tcgcttcacg cggggttaag ccctctaaca cttcgttcgt tgccatacgc
     2401 aggctctccg cagtggcgga atccaatggt aattcaaggc tttcgtcttg aatgaaatca
     2461 cctaaatgtg aatcgtcatc gtcaccgatt ggtgtttcca ttgaaatcgg ctctttggca
     2521 attttcaata ctttgcggat tttatcttcc ggcattccca tacgttctgc cagttcttcc
     2581 ggcgttgcct cacgccccat ttcttgtaaa cattggcgtg aaatacggtt gagtttgttg
     2641 atagtttcaa tcatatgcac cggaatacga attgtacggg cttggtcagc aatcgaacgg
     2701 gtaatcgctt gacgaatcca ccaagttgca taagtcgaga atttataacc acggcggtat
     2761 tcaaatttat ccaccgcttt cattaaaccg atgtttcctt cttggattaa atctaagaac
     2821 tgcaagccac ggttagtgta ttttttcgca atcgaaatca ccaaacgtaa gttagcttcc
     2881 accatctctt ttttcgcacg gcgggctttt aactcaccat tggaaatacg cccgccaatt
     2941 tcacgaattt gagcaatggt taagcctgat gtttgctcta aattctgtaa gttagcaata
     3001 ttgacacgga tattatccac ataattagct aatttcggtg ctgcattttt cttggcatta
     3061 atcgcttttt caatccacgc ttcgcttaat tcgtgccctt ggaatgcttt caagaaatcc
     3121 gcttttttca tgccggcata ttccactgca tagcgttgaa tttgacgttc ttcagtgcga
     3181 acctgcttca tcagtttttg catagaagca accaataagt caaattgctt cggcaccaaa
     3241 cggaattcac ggaaaatatg agataaaatt tcaatttgct ctttcgattt tttactggtt
     3301 cgcccgtgtt tttgaatgac caataacact ttttcatgtt ggctgcgtag ggcttcaaat
     3361 ttttcacggg caagttccgg gtcaattgag ctgtcatcac tgctgtctga actgctatca
     3421 ccgttttcat cttcgttttc gtcatctaca tcaggcacat cagaagattc ttcatcttca
     3481 cctaaagcag cctcaatatc ctctaacggt gcttcttcta aaatattagg atcgacaaat
     3541 gctgtcacta aatcagaaag gcgcatttca ccggtttcga cttgagcata gcaggctaac
     3601 aggccactta aggcttccgg atattccgca atcgcacatt gaacttcgtt aatcccttct
     3661 tcaatgcgtt tagcgatatc aatctcacct tcacgggtta aaagctcaac tgtccccatc
     3721 tcacgcatat acatacgcac agggtcagta gttctaccta actctgattc tacggttgaa
     3781 agcactttgg tggcttcttc taccacatct tcatcggcga tattttcagc gagtagtaaa
     3841 tcatccgcat caggggcagc ctctaaaact tggataccca tatcgttgat catctgaata
     3901 atatcttcga tctgctccgc atccaccaac tcttccggta agtggtcgtg cacttcagaa
     3961 agcgttaaat atccctgttc acgcccttga ctgatcagaa gttctaattg agattgctgt
     4021 tctaattgtt gatctgcttg gttttccata gtctatccgt ttattatgcg agcaaaaata
     4081 gtagtggatt ataccactaa atttggttcg acttcataaa ttcacaaaaa aattctattt
     4141 ttctaattta cacattactt tgatgcttat gagaacagag aggtgtagca agagagataa
     4201 ataactttct ttttagatag aattaattat tttcttcgtt catcacttct gccaaatctc
     4261 ccctaacccc tctttgctaa agaggggaat tattcgagct taaaattcta ctgaaacatc
     4321 aaaagagcat actctttttt ctcatcatcg gttaagccca cggttcggtc tttggcaatt
     4381 agctcttctt tgcgtaattc cactaatttg gcgtagagaa aatcgagagt gtcaataaat
     4441 gttcgttcga tattttcggg taactctaaa tgatcccaat ttgcgagggc ttcaacaatc
     4501 ttatattggg gatcttcccg ataatgttca agtagcccgc ccatactgat acctgcattt
     4561 tgctgacaag ttgcgacaat atcggttaat aatgtaaagc caggatcttt caaggaggct
     4621 aaagcactta catcgggtac gaatttcaca aaatacggat tttgaatcaa taacgcaatc
     4681 aacagccgca ttggcgtacg tttcattttg accgcttgat gtaaaggctt ctctgccgct
     4741 tttttcgtcg gtaacagagc ttctatctgc gttgggtcta aaatgcctaa tttctgtcct
     4801 aaaatattgc gtaaatacac tcgcagcatc tcgcccggaa tacgattaat taacggcaca
     4861 gctaaagcag cgagcttgga ttttccctct tttgaagaga gatccacatc cgccagcaaa
     4921 ctgtcaaata agaaatcgct taatggcttg gcattctgtg ccacgtactg ctcaaatcct
     4981 tctttacctt gagaacgaac aaaggtgtcc gggtcttcgc catcgggcaa gaaaataaat
     5041 ttcagttggc gaccgtcttg caaataaggc aacgcatttt ctaacgcccg ccaagctgca
     5101 tctcgaccgg ctctatcgcc atcataacaa cagacaattt gctcggtgca acgaaacatt
     5161 tgctgaattt gttcaccggt agttgccgtg cctaaagagg cgactgcatt gtccacgccg
     5221 aattgggcaa gggcgaccac gtccatatag ccttccacca cgaacagata atccggcgtt
     5281 tcattgactt tcagtgcttg atacaagcca tataactcac tgcctttatg ataaatcacc
     5341 gtttcgggcg agttcatata tttcgcccct tgctcatcat tcggcaacac ccgcccacca
     5401 aaggcaatta cccgtccacg cttatctcga atcggtaaca ttacccgatt gcgaaattta
     5461 tcgtaaattc gaccgctatc atttttggtt aaaacgcccg tatcgagcaa tttctgaata
     5521 tcttcttgtg attgcccaaa agtgcgtagg gctgcatcaa aggcattcgg agcaaaacca
     5581 atttcaaagc gttcgataat ctcttcagac aaacctcgtt gctgtaaata gctttgtggc
     5641 tcaatctggt gcgataaatt ctgctggtag aacttcgcca ccgcttccat caattcataa
     5701 agattacgct tagttttaaa attagcttga ggcttgccgt cacgttgaat cacattctct
     5761 ctcggtactt ccagcccgtg catagctgcc agttcttcaa tcgcttccgg aaattcgagt
     5821 ttgtcataat ccatcaaaaa ggtgatgaca ttgccgtgta cgccacagcc gaaacaatgg
     5881 taaaactgtt ttgattggct taccgaaaag gaaggggttt tctcgtgatg aaaaggacag
     5941 caggcttgat aatctcgccc agcctttttg agtttgaccc gactgttgat gacctcaaca
     6001 atatcggtgc gagcgataag atcatcaata aatgaacgtg gaattgtgcc tttcattgat
     6061 ttcctcctgc tctttgaatg atacaagcgg agcaattttg caaatttttt gcaaaaaaca
     6121 accgcttgaa atagaaacgt taaaaacacc aaaaccgtga gcctaacgga atcacggttt
     6181 cgatatagtt tgctaaccag cctaactaat tagtataaac gtgtgttacg tgcgttctca
     6241 cggaaattac gtttagcgtg acgtttagca agtgatgctt tttcacgttt acgaattgta
     6301 gttggttttt cgtagaattc acgagcacga acttcagcta agatacccgc tttttcgcaa
     6361 gagcgtttga aacgacgtaa tgctacgtca aatgattcat tttcacgaac tttaattact
     6421 ggcatatgcc atcacctcag aaaaatatga ttaaattaaa ccgcacacaa ttttggcggc
     6481 atagaacaaa aaaggtagtg cattctacct taagcactca cataagtaaa gtagtattgt
     6541 ggcataaaac cgccgtatta tgttgcattt ttctacagtt cgaataattt ctcggtcgaa
     6601 aaacttaatg ccggaaagac caccgaagtt gccggttcat cttctgccac cggctgaata
     6661 ccgatatatt taccgttttc caacacaaaa atctgaatag tgcgggtttc gggagaaaca
     6721 atccaatact ctttcacccc ttgttcctgg taaagctcgt atttatcttt catctccctt
     6781 ttggtattac ccggagaaag gatttccacc actaaatccg gtgcccctac acagcctctt
     6841 tcatctaact tactgacatc acaaattaca caaagatcag gctgcactac agttttgact
     6901 tttccgtttt catcgggaaa ccgaacgtca aaaggcgcag aataaatctg gcattggtga
     6961 ttgtcaaaaa cctctaaaaa ttttgctgtc atcttcatag aaattcgctg atgaaaacga
     7021 gtaggagccg gagacatcgc cacaattttc cctttgataa tctcaacccg ctctttaaat
     7081 ttccatagca aataatcagc gtaactgtaa cttttttgta agtccagttg atttaagctg
     7141 gtaatttcgt tcattggtaa taagctcata ccgcctcctt tatcagaaat ataaagatat
     7201 tatgccgaat aatccctcaa attcaacaaa actttcgcta aaaagagata gataaccttt
     7261 atacccttag tataaaatga cagcaatttt cattgtttta ggatatgtcg atgcacctta
     7321 acttcaaaaa aatagacaac tcacatattg ctcttttcta taatgtacta gtcgccacat
     7381 tttttgtcac tgttttaacc tttaagaaag gttatagtta tgtgccaatg gcattaggta
     7441 ttatcgctac acttagtttc ttattttatc gtttcaaatt aaaacaaaag tggttacttg
     7501 ataaagagga taaatttttt attttctcgc ttattggcta ttttctaacg tttgcaatgt
     7561 ctgctatttt taatggcgat ggcattcgtg aaattgataa tcctatccag attttattgc
     7621 ttatcccttt aatcatcctc tttagacttt acccgattaa aaaagaaact gtattgcact
     7681 ttataccgat tggagcgttg gtagctggt
//
LOCUS       DANLUQ010000120         7644 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.121, whole genome shotgun sequence.
ACCESSION   DANLUQ010000120 DANLUQ010000000
VERSION     DANLUQ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7644
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.121"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            19..1446
                     /gene="hldE"
                     /locus_tag="PXB27_001950"
     CDS             19..1446
                     /gene="hldE"
                     /locus_tag="PXB27_001950"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HDL5346709.1"
                     /translation="MMMQYSPKFNNAKVLVLGDVMLDRYWFGATNRISPEAPVPVVKV
                     QDIEERAGGAANVAMNIASLGVPVTLHGLIGDDDAGRALDKLLNSHQIQNHCVVINSH
                     PTITKLRILSRHQQLLRLDFEEGFHNVDSTALLAKLSQEITAYGALILSDYGKGTLES
                     VQQMIQIARNAKVPVLIDPKGTDFERYRGATLLTPNMSEFEAVVGHCKDEDEIVSKGL
                     KLIADYDLSALLVTRSEKGMTLLRPNHEPYHLPTQAKEVYDVTGAGDTVISVLATAIA
                     DGRAYEEACYLANAAAGVVVGKLGTSTVSPVELENAIHQRTATGFGVVSEAELKAIVE
                     ETKKRGEKIVMTNGCFDILHPGHVSYLENARKLGDRLIVAVNTDASVKRLKGEARPIN
                     DLAARMAVLAGLASVDWVVPFDEDTPQRLISEILPDLLVKGGDYKPEEIAGSQEVWAN
                     GGDVRVLNFENGCSTTNVIKKIQAL"
     gene            1460..2014
                     /gene="pyrR"
                     /locus_tag="PXB27_001951"
     CDS             1460..2014
                     /gene="pyrR"
                     /locus_tag="PXB27_001951"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDL5346710.1"
                     /translation="MAREKIIIDTEQFQRTISRISHQIIEKHGDLQDVVIVGIKRRGA
                     EIAELIQKRIEDLAQTRLPYMALDITFYRDDLDLIYQDPVFMGADNQLNLNGKKVILV
                     DDVLFTGRTIRAALDALLDFGRAARIELVIFVDRGHRELPIRADYVGKNIPTAKNEQI
                     QVQTLSYDGINQVVLVPAANKESA"
     gene            2087..3037
                     /locus_tag="PXB27_001952"
     CDS             2087..3037
                     /locus_tag="PXB27_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007523149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL5346711.1"
                     /translation="MKMKLVKSLFAVAVATMSLTQTAHAFEERVVALVNDTPIMQSQV
                     QRVLGKKKATETAQRAAIDQVIDDMLVQQAMKEAGVKVSPAAVNQAVENVAIQNGITY
                     GQLLDALDYQGITLEQFKRNIAQQMAMEQVRHISISKSIQVAPQQVQALAKELMAKDK
                     AAGKLKTVSGKEHRISHILLKTNPILNDAQAKAKLSSLTTDINSGKISFEEAAKTHSV
                     DYASGADGGDLGFNFLEIYDPAFANVASKIKPNQISAPFKSQFGWHILKVTDTRNGDR
                     TEDAYHQRAYQQLVNKQAEEASKDWVKALRKTANIQYVGQ"
     gene            3099..3965
                     /gene="rsmA"
                     /locus_tag="PXB27_001953"
     CDS             3099..3965
                     /gene="rsmA"
                     /locus_tag="PXB27_001953"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDL5346712.1"
                     /translation="MSSTNSKKHLGHTARKRFGQNFLHDMNIIHSIVSAINPKNGQFL
                     LEIGPGLGALTEPVAELVDKLTVVEIDRDLAERLRHHPFLNQKLTIIEQDALRFNFRD
                     YFNQLNLDEDSVRVFGNLPYNISTPLIFHLLTFHDLIQDMHFMLQKEVVKRLCAAPNS
                     KAYGRLTIMAQYYCQVIPVLEVPPTAFKPAPKVDSAVVRLVPYNQLPYPAKDVYWLNR
                     VTTQAFNQRRKTLRNALSTLFSAEQLDALGVDLTARAENLTIADYVRLANWLHDNPPA
                     IDTTEELVDEDC"
     gene            complement(4076..4705)
                     /gene="upp"
                     /locus_tag="PXB27_001954"
     CDS             complement(4076..4705)
                     /gene="upp"
                     /locus_tag="PXB27_001954"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDL5346713.1"
                     /translation="MKIVEVKHPLVKHKLGLMREAEISTKDFRELANEIGSLLTYEAT
                     KDLEMESVEINGWSGEKIAVERIKGKKVTVVPILRAGLGMMDGVLEHIPSARISVVGI
                     YRDEETLKPVPYFSKLANDVDERLAIITDPMLATGGSMVATIDLLKKAGCKQIKVLVL
                     VAAPEGIKVLEAAHPDVELYTASIDSHLNENGYIIPGLGDAGDKIFGTK"
     gene            complement(4730..6415)
                     /gene="fadD"
                     /locus_tag="PXB27_001955"
     CDS             complement(4730..6415)
                     /gene="fadD"
                     /locus_tag="PXB27_001955"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HDL5346714.1"
                     /translation="MEKIWLNNYPPYSPKTIETDQYESLVEMFEAAIQRHPDIPAYIN
                     MGQVLTFRKLEERSRSFAAYLQNELRLKKGDRIALMMPNLLQYPIALFGALRAGLVVV
                     NVNPLYTPRELEHQLNDSGAKAIVIVSNFAATLEKIVFNTEIKHVILTRMGDQLSFGK
                     RTLVNFVVKYIKKLVPKYKLPHAVSFREALSIGKQRQYVKPNIVSQDLAFLQYTGGTT
                     GVAKGAMLTHQNLIANMMQAKWIVEPLLGNSANMIGLVPLPLYHVLALSMNCLLFIEL
                     GLTGLLITNPRDIPGFIKELKKYKVSVITGVNTLFNALLNHESFKEVDFSHLKLSVGG
                     GAAIQSSVAKRWHDLTGVHIIEGYGMTECSPLISATRGDSVEYSGSIGVPVPNTDIRI
                     VDDEGKEVPLGERGELVVKGPQVMQGYWNRPDETAQVLQDGWMATGDIVVMGDDLNLR
                     IVDRKKDMIIVSGFNVYPTEIEEVISQNPKVNEVVAIGVPSEASGESIKIFVTKKDES
                     LTRDELRTYCRQFLTGYKIPRDIEFRDELPKSNVGKILRRVLRDEELAKVNKA"
     gene            complement(6393..6857)
                     /locus_tag="PXB27_001956"
     CDS             complement(6393..6857)
                     /locus_tag="PXB27_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HDL5346715.1"
                     /translation="MMIEQATVVEYQNGVAIVQCYAKSSCGGCVAQSACGSKALSALA
                     GEKIDPRFRIEVDEPLKVGDKVELGLAENILLKSVFLIYGIPLLVLVMTAVGFSQFFA
                     NELIVLFIMLLSTGITFWAVRKLIHKHSKQANFSPIFLGKVLGDYNGKNMAK"
     gene            complement(6854..>7644)
                     /locus_tag="PXB27_001957"
     CDS             complement(6854..>7644)
                     /locus_tag="PXB27_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HDL5346716.1"
                     /translation="HAFTHNKEYAQLLNLDHSREEIILKNNKVSYLGYNFRPFSLNSS
                     QILDNLPNVLYTDYANLKGYAFLDAGKDRISDRITKVIRMKPNDTLRYAYILWIDEET
                     HLLLKSQVLNMDNLVLEEFRVLQLYQSPELDRIANVIESLMLPTLTTVKKEEFPKSDG
                     WQVKWLPTGFRLIQQQTVTGATYQVESEYVDSRVYSDGLSTLTIYIMPSQGVSFNEYA
                     WQQGKLTILNQTINDRDIVIIGDVPLQSAKQIMNNIGFKEGAQP"
BASE COUNT         2253 a         1662 c         1510 g         2219 t
ORIGIN      
        1 taacagagaa tattaactat gatgatgcaa tactctccaa aatttaataa tgctaaagtg
       61 ctggtgcttg gcgatgtaat gttagaccgt tactggttcg gggcaaccaa tcgaatttcg
      121 cctgaagccc ctgtgccggt ggtgaaagta caagatattg aagagcgtgc cggcggtgcg
      181 gcaaacgtgg cgatgaacat cgccagcctt ggcgtaccgg taacattaca cggcttaatt
      241 ggcgatgatg atgccgggcg tgctttagat aaattactta attcccacca gatccaaaat
      301 cattgtgtgg tgattaattc gcacccaacc atcacaaaat tgcggattct ttcccgccat
      361 cagcaattat tgcggttgga ttttgaagag ggttttcaca atgtagattc taccgcccta
      421 cttgcaaaac tttcgcaaga aattaccgct tacggagcat taattctctc ggattacggc
      481 aaaggcacat tggaatcggt gcaacaaatg atccaaattg cccgcaatgc taaggtgccg
      541 gtgctgatcg acccgaaagg cacagacttt gaacgctatc gtggggcaac attgcttaca
      601 ccaaatatgt cggaatttga agcggtggtt gggcattgta aagatgagga tgagattgtc
      661 agcaaagggt taaaactgat tgccgactat gatttatctg cactattagt tacccgttcg
      721 gaaaaaggaa tgaccttact tcgcccgaac cacgagcctt atcatctgcc gacccaagcc
      781 aaagaggttt atgatgtaac cggtgcaggc gataccgtaa tcagtgtatt agccactgcg
      841 attgccgatg gcagagccta tgaagaagcc tgctatttag ccaatgctgc cgccggtgta
      901 gtcgtaggta aattagggac ttcaaccgtc agcccggttg agctggaaaa tgcgattcac
      961 caacgcactg ccaccggttt tggcgtggtt tctgaagccg aattaaaagc gattgtggaa
     1021 gaaacgaaaa aacgtggcga aaaaatcgtg atgacgaacg gttgctttga tattcttcac
     1081 cccggtcacg tttcctattt ggaaaacgcc cgtaaattag gcgaccgatt aattgtagcc
     1141 gtcaataccg atgcttcggt aaaacgccta aaaggcgaag ctcgcccgat taatgattta
     1201 gctgcccgta tggcagtttt agctggttta gcctctgtag attgggttgt gccttttgat
     1261 gaagatacac cacaacgttt aatcagtgaa atcctacctg atttattggt aaaaggtggt
     1321 gattacaagc cggaagagat tgccggcagc caagaagtat gggcaaacgg cggcgatgtg
     1381 cgagtattga actttgaaaa cggctgttct accactaatg tgatcaagaa aattcaagca
     1441 ctataatggt aataagcgaa tggcgagaga aaaaatcatt attgataccg agcaatttca
     1501 gcgcacaata tctcgtattt cacatcaaat tattgaaaaa cacggtgatt tgcaagatgt
     1561 agtgattgtc ggcatcaaac gtcgaggagc tgaaattgcc gagttaatcc aaaaacgcat
     1621 tgaagattta gctcaaacac gcttgcctta tatggcgtta gatattacgt tctaccgtga
     1681 tgacctagac ttaatttatc aagatcccgt atttatggga gccgataatc agctaaatct
     1741 gaacggtaaa aaagtgattt tggttgatga tgttctgttt accggcagaa ccattcgggc
     1801 ggcacttgat gcactcttag attttggcag agctgccaga attgaactgg tgatttttgt
     1861 cgatagaggt catcgggaat tgcctatccg agccgattat gtcggaaaaa atattccaac
     1921 ggctaaaaac gagcaaattc aggtgcaaac gctctcgtat gatgggatta accaagtggt
     1981 cttagttcct gctgccaata aagaaagtgc ttaaattaag tgaacctttt cgctaaactt
     2041 atctctaagg aaaagttact gcttaattac ctccattagg attgttataa aaatgaaatt
     2101 agtcaaatca ttatttgctg ttgccgttgc aacaatgagt cttacccaaa ccgcccacgc
     2161 atttgaagaa agagtggtgg cgttagtgaa tgatacccct attatgcaaa gccaggttca
     2221 acgtgtgctt ggcaagaaaa aggcgacaga aactgctcaa cgtgcggcta ttgatcaagt
     2281 gattgatgat atgttggtgc aacaggcaat gaaagaagcc ggcgttaaag tcagcccagc
     2341 tgcggtaaac caagcggttg aaaatgtagc aattcaaaac ggcattactt acggtcagtt
     2401 attagatgcg ttagattatc aaggtattac acttgaacaa ttcaaacgta atattgctca
     2461 acaaatggca atggaacaag ttcgccatat cagcattagc aaatcgattc aggttgcacc
     2521 acagcaagtg caagctctgg cgaaagagtt aatggcgaaa gataaagcag caggcaaatt
     2581 gaaaaccgtt tctggcaaag agcatcgcat tagtcatatt ttgctcaaaa ccaacccgat
     2641 tttaaatgat gctcaagcca aagcaaaact tagcagctta actactgata ttaactcagg
     2701 caaaatcagc tttgaagaag cggcaaaaac ccattctgtt gattatgcct caggggcaga
     2761 cggtggagat ttaggtttta acttcttaga aatttacgat ccggcatttg cgaatgttgc
     2821 atcaaaaatc aaaccaaacc aaatttctgc tccgtttaaa tcgcaatttg gttggcatat
     2881 tttaaaagtc accgacactc gtaacggaga cagaaccgaa gatgcttacc accaacgtgc
     2941 ttatcagcaa ctggtgaaca agcaagccga agaagcctct aaagattggg taaaagcgct
     3001 tagaaaaacc gccaatattc aatatgtcgg tcaataatta aaatttttaa gaacctcgca
     3061 taatgcgggg ttcttttctt atttcaaaaa ggataacaat gagttctaca aattccaaaa
     3121 aacatttagg tcataccgct cgtaagcgtt tcgggcaaaa ctttttgcac gatatgaaca
     3181 ttattcacag catagtttca gccattaacc cgaaaaacgg acaattttta ttagaaatcg
     3241 gtccgggttt aggagcatta accgagccgg ttgccgaatt ggtagataaa ctcacggtgg
     3301 ttgaaattga ccgtgattta gcagagcgtt tacgccacca cccgttttta aaccaaaaac
     3361 tcaccattat tgagcaagat gccctacgct ttaatttccg tgactatttt aaccaactca
     3421 acttagacga agactcggtg cgtgtgtttg gtaacttacc ttacaacatt tctacgccct
     3481 tgatattcca cctgctgacc tttcacgact taattcaaga tatgcacttt atgttgcaaa
     3541 aagaggtggt gaaacgttta tgtgccgcgc caaacagtaa agcatacggg cgtttaacca
     3601 ttatggctca atactattgc caagtaattc cggtgctgga agtgccgcca accgccttta
     3661 aaccggcacc aaaagtcgat tctgcggtag tgcgtttagt gccttataac caactgcctt
     3721 atccggcaaa agatgtgtat tggctcaatc gggtcacgac tcaagccttt aaccaacgcc
     3781 gtaaaacctt acgcaatgct ctttcaaccc tgtttagtgc ggagcaactg gacgccttag
     3841 gcgttgattt aaccgcccga gcggaaaacc ttaccattgc cgattatgtg cgtttggcta
     3901 actggctaca cgacaacccg ccggcaattg atacaaccga agaattggtt gatgaagatt
     3961 gctaatcaat aaattacaag cggtcatttt tctaccaaga tttgcaaatc tttagtgaaa
     4021 aatggccgct tgtttaaatc agctttactt gttttgctgt tacttagttt tgatgttact
     4081 tggtgccgaa aattttatcc cctgcatcgc ctaagcctgg aatgatatag ccgttttcgt
     4141 ttaagtgact atcgattgag gcggtgtaga gttcaacatc tgggtgggcg gcttctaata
     4201 ctttaatccc ctctggagcg gcaactaaca ctagcacttt aatctgctta cagcctgctt
     4261 ttttcaggag gtcaatggtt gctaccattg agccaccggt tgcaagcatt gggtcggtga
     4321 taatggctaa acgctcatca acatcgttgg ctagtttgga gaaatacggc acgggcttta
     4381 aggtttcttc gtcacgataa atcccaacca cactaattct ggcacttgga atatgctcta
     4441 acacaccgtc catcatacct aagccagcac gcagaatcgg cacaacagtc acttttttgc
     4501 ctttaattcg ctcaaccgca attttctctc cgctccagcc attgatctcc acactttcca
     4561 tttccagatc tttggttgct tcgtaagtaa gcagactacc aatttcattc gctagctcac
     4621 ggaaatcttt ggtgctgatt tccgcttcac gcattaagcc caatttatgc tttacaagtg
     4681 ggtgttttac ttcaacaatt ttcattttaa gctccttaat tagtgtaatt taagccttat
     4741 tcacctttgc taattcttcg tctcttaaca ctcggcgaag aattttgccg acattacttt
     4801 ttggtaattc gtcacggaat tcgatgtctc ttggaatttt gtagccggtt aagaattgac
     4861 gacaataggt tctcaattca tctcgagtta aactttcatc ttttttggtt acaaaaattt
     4921 taatgctttc gccggaagcc tcactcggca caccaatcgc cactacttca ttcacttttg
     4981 ggttttggga aatcacttct tcaatttcgg ttgggtacac attaaaaccg gagacaataa
     5041 tcatatcttt tttacgatca acaatgcgta agttcagatc atcccccatt accacaatgt
     5101 caccggttgc catccaacca tcttgtagca cttgtgccgt ttcatcagga cggttccaat
     5161 agccttgcat tacttgcggc cctttcacca ccagctcgcc ccgttcgccg agtggtactt
     5221 ctttgccttc atcatccaca atacgaatgt cagtatttgg cactggcacg ccaatagagc
     5281 cggaatattc caccgaatcg ccacgggttg cactaatcag cggagaacat tctgtcattc
     5341 cataaccttc gataatatgt acgccggtta aatcgtgcca gcgttttgcc acgctagatt
     5401 ggatcgctgc accgccacct acacttaatt ttaggtgtga aaaatcaacc tctttaaaac
     5461 tttcgtgatt aagtaatgca ttaaacaatg tatttacccc agtaatcact gaaactttgt
     5521 acttttttaa ttctttaata aagcctggaa tatcgcgcgg attagtaatt aataatccgg
     5581 taagtcctaa ttcgataaag agcaagcaat tcatcgaaag tgctaagacg tgatacagcg
     5641 gtaaaggtac caatccaatc atattggcag aattacccaa caatggctca acaatccatt
     5701 ttgcctgcat catattagca attaagtttt gatgagtcag cattgcccct ttcgctacac
     5761 ctgtagtacc gccagtgtat tgtaaaaagg cgagatcttg gctgacaata ttcggtttta
     5821 catattgacg ctgtttgcca atgcttaagg cttcacggaa gctgaccgca tgtggcaatt
     5881 tatatttcgg cactaatttt ttgatgtatt tcaccacaaa attgactaag gtgcgtttgc
     5941 cgaatgaaag ttggtcgccc attcgggtca aaataacgtg cttaatctca gtattaaaga
     6001 caattttttc taaggtagcc gcgaagttcg agactatcac aatcgctttc gcaccactgt
     6061 catttaattg atgttctaat tcacgcggcg tataaagcgg gttgacattc accaccacaa
     6121 gcccggcacg taatgcgcca aataaagcga tcggatattg caataaattc ggcatcatca
     6181 atgcgattcg atcacctttt ttcaggcgta attcattttg caaataagcg gcaaacgagc
     6241 ggctacgttc ttccaattta cggaacgtta aaacctgccc catattaata taagctggaa
     6301 tatcagggtg gcgttgtatc gctgcttcaa acatttccac taaagattca tattgatctg
     6361 tttcaatcgt ttttggtgaa taaggcggat aattatttag ccatattttt tccattatag
     6421 tctcctaaaa ccttacctaa aaagataggt gaaaaatttg cttgtttgct gtgtttatga
     6481 atcaattttc ttacagccca gaatgttata cctgttgaaa gcaacattat aaataaaaca
     6541 ataagctcat ttgcaaaaaa ttgcgaaaat ccgaccgctg tcatcacaag aaccagcaga
     6601 ggaatgccat aaattaaaaa cacgcttttg agcaagatat tttcagctaa ccctagttca
     6661 accttatcgc ctacttttag gggttcatct acctcaatac gaaaacgagg gtcaattttt
     6721 tcacctgcta atgctgataa tgctttgctg ccacaagcag actgagctac acagccacca
     6781 cagctacttt tagcataaca ttgtacaata gccacaccat tctgatattc caccaccgta
     6841 gcctgttcga tcatcatggc tgagctcctt ccttaaaacc aatattgttc ataatttgct
     6901 tagccgattg caacggcaca tcaccaataa tcacaatatc tctatcatta atcgtttgat
     6961 ttaaaatcgt taatttacct tgttgccaag cgtactcatt aaaactcacg ccttggctag
     7021 gcataatata aatagttaat gtagataaac catcactata tactctgcta tctacatact
     7081 cactttccac ttgatatgtc gcacctgtga ctgtttgttg ctgaattaac ctaaaccctg
     7141 tcggcaacca cttcacctgc caaccatcag acttaggaaa ttcttctttt ttcactgtcg
     7201 ttaatgttgg taacattagt gattctatta catttgcaat cctatccaat tctggagatt
     7261 gataaagttg caaaactctg aattcttcta ataccaaatt atccatattc aagacttggc
     7321 ttttaagtaa taaatgtgtt tcttcatcaa tccatagtat ataggcatat cttaaagtat
     7381 catttggctt catacgaatt acctttgtaa ttcggtcaga aatacgatct ttaccagcat
     7441 ctaaaaaggc ataacctttt aaatttgcat aatctgtgta aagtacatta gggaggttat
     7501 ctaaaatctg tgaactattt aaactaaaag gacgaaaatt ataccccaaa tagctgacct
     7561 tgttattttt taaaataatt tcttctcggc tgtgatctaa attcagtaat tgggcatatt
     7621 ctttattatg ggtaaaagca tgac
//
LOCUS       DANLUQ010000121         7582 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.122, whole genome shotgun sequence.
ACCESSION   DANLUQ010000121 DANLUQ010000000
VERSION     DANLUQ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7582
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.122"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            6..737
                     /locus_tag="PXB27_001958"
     CDS             6..737
                     /locus_tag="PXB27_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005615381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL5346717.1"
                     /translation="MKLITAMILPPFNIILLWLLALILFSLGYKYAAYFFALLGILIL
                     YIFSIPYTSQKLGDSLVEAEPFTIEKYKNAQAIVVLGGGLRDSQELFGKLAITGIPLE
                     RMRYAAYLHKETGLPILATGSSPNGTSEAKVMADEFKMFFNIETKWLENKAKTTKENA
                     IFSREILEKENIHKIILVTNQWHMKRAEMLFSQQGFEVMSASVGSGDTPDHYKLNFMH
                     FIPQAGAMNSNMLALKEWIGYWKEK"
     gene            complement(814..3012)
                     /gene="priA"
                     /locus_tag="PXB27_001959"
     CDS             complement(814..3012)
                     /gene="priA"
                     /locus_tag="PXB27_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDL5346718.1"
                     /translation="MALNVPLHRYFDYLLPPAFNACQGGRVVVPFGRQTKIGIVVDFP
                     QTSDVPTEKLKPIKAALDSSPIFDSEMWQLLNWAARYYHAPIGEVLTGALPIKLRNGD
                     STERVQPNYFVVTEQGRKSLIIGENKKAKKQVERLTELAECAKYFEKPTACSPSAWKG
                     LLEKGYIEKIDVPFMAQAWQDSLGEFPLVNTNNRLILNKQQTLVVSRLNAQTTFASFL
                     LNGVTGSGKTEVYLQLIETVLKREEQVLLLVPEIGLTPQTVQRFKARFNVEIDVLHSN
                     MNETERLNAWLRAKNGESAIVIGTRSALFTQFQKLGVIILDEEHDSSFKQQDGWRYHA
                     RDLAVVRAKNANIPIVLGSATPSLESLHNVQNGKFIELTLTARAGNAQLANQQIIDLK
                     TQRVTAGLSERLLSMMKTQLEQGNQVMLFLNRRGFAPVLLCHECGWVCECEACNKPFT
                     YHQKQKVLRCHHCATQRVIPRQCGHCGSTHLITTGVGTEQLEQVLNQQFPHYQVTRID
                     RDSTARKGALENHLTAIREGKSQILIGTQMLAKGHHFPNVTLVAIVNVDSALFSTDFR
                     AEEKLAQLYIQVAGRAGRAEKKGEVVLQTHYPDHPLLKTLLEQGYRAFADEALKMRQL
                     MSLPPFSSQVLFKATGKDNQQTLQLLEQLADYFSQKIQQQNGQGFQLPPPFSAPMAKK
                     AGHYRWLLLIQHPLRGALQQLLDAFDQERESLNIPNNIRLNIDIDPQEIS"
     gene            complement(3024..3636)
                     /locus_tag="PXB27_001960"
                     /pseudo
     CDS             complement(3024..3636)
                     /locus_tag="PXB27_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005818858.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
     gene            complement(3629..4528)
                     /gene="rarD"
                     /locus_tag="PXB27_001961"
     CDS             complement(3629..4528)
                     /gene="rarD"
                     /locus_tag="PXB27_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDL5346719.1"
                     /translation="MLKGILLSLSASVLFGCMYYLAIFLKPLAGVSIFGIRMLVTIPF
                     LFLALYLFKKQNEFWVFLQRLKQEPYLLLVILITASIVGGQMWLFLWAPNSGKAIEVS
                     MGYLLMPIIMVAFGKVVYKETLSLNKWLAIVVAFIGVASNILFTGKISIASLFVCTGY
                     PIYFYLRRKFGLSHLHSFVVEILYLLPVACYFISQTDMQYVETQNLNIYYFIALLGLI
                     SGTALISYTLASTILPFNLLGLLGYVEPLLMLVVSFLIGERLANESYLLMICLLISIS
                     FLVLDGMFALRQQRKRKLSVNNE"
     gene            complement(4755..5972)
                     /locus_tag="PXB27_001962"
     CDS             complement(4755..5972)
                     /locus_tag="PXB27_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL5346720.1"
                     /translation="MDRFPKSDKLAQVRYDIRGPIHKEALRLEEEGHKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIVQYYQSKGMRGMDVNDVYIGN
                     GVSELITMSMQALLNDGDEILIPMPDYPLWTAASTLAGGKAVHYLCDEENEWFPDLED
                     IKSKITPRTKGILVINPNNPTGAVYSRQILLEIAELARQHNLIIFADEIYEKIVYDGA
                     VHHHIAALAPDVLTVTYNGLSKAYRVAGFRQGWMVLSGPKHQAKGFIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFVLPGGRLLEQRNKMHELLVQIPGISCVKAKGALYM
                     FPKIDTEMYGIKDDQKFIYDLLRQEKVLLVQGSGFNWKKPDHFRVVTLPYAHQIEEAI
                     GRLANFLKTYRQD"
     gene            6160..7464
                     /locus_tag="PXB27_001963"
     CDS             6160..7464
                     /locus_tag="PXB27_001963"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HDL5346721.1"
                     /translation="MLIFHQLKQELTKSYSKVGREQGLVAIQASMPLLEANIPLLGWL
                     KTQKTSYPHFFLQYRDTDQTIATIGTLRQFYTLEAAQSFIQQHHFPLIGGLQFEGNTQ
                     FILPQFSLVKNQQNLTACFYFDSDNFEQQAVIFEQFLANFSQITELGLTDNPLVSMSA
                     TSHFEQWKANIEKAIVSIEQGDFQKVVLANAMRLHFEQPICAYSLLAQSQKTNLGCYH
                     FLWAESADVAFLGSTPERLYQRQGLHFSTEALAGTAAVTTSEVQTELNAQWLLSDPKN
                     IYENQLVVDDIENQLKDCVTEFAVSEAKIKRLHNVQHLRRQIQATLKPDTSDSDCLSK
                     IHPTAAVAGLPRQKAKGFITENEPFTRHWYAGTLGVMGNEEAEFCVTLRSALISQNSI
                     TLYAGAGIVSGSDPQSEWQEIKRKAQGMAKLLDVDLVTKGEL"
BASE COUNT         2295 a         1662 c         1488 g         2137 t
ORIGIN      
        1 agttcattaa actgatcacc gcaatgatcc tcccgccatt taatattatt ttgctgtggt
       61 tgttagcatt aatcctcttt tctttaggct ataaatacgc tgcttacttc tttgccttgt
      121 taggtattct gattttatat atcttcagca ttccctatac ttcacagaaa ttaggggatt
      181 cattagtcga agcagagcca tttaccatag agaaatataa aaatgctcaa gcgattgtgg
      241 tgctaggtgg cgggctacga gacagccaag agttattcgg caaactggca attaccggta
      301 ttccgctgga aagaatgcgt tatgccgctt atctgcataa agaaaccggc ttaccgattt
      361 tagcgaccgg aagcagccca aatgggactt ctgaggcaaa ggttatggca gatgaattta
      421 aaatgttctt caatattgag accaaatggc tggaaaataa ggcgaaaacc accaaagaaa
      481 atgcgatttt cagccgtgag attttagaaa aagagaatat tcataaaatc attcttgtta
      541 ccaaccaatg gcatatgaaa cgggctgaaa tgctgtttag ccaacaagga tttgaggtga
      601 tgtctgccag cgtcggttct ggcgatacgc ccgatcatta caaactcaac tttatgcact
      661 ttattccgca agccggtgca atgaatagca atatgctggc attaaaagag tggattggtt
      721 attggaaaga gaaataaccg aagataaata gcagcaaggc aggttttcac ctgccttctt
      781 tttgcaaaaa atcagtcaaa aacaaccgct tgtctaacta atctcttgtg ggtcaatgtc
      841 gatattcagc ctgatattat tcggaatgtt taaactttcc ctttcttgat caaaggcatc
      901 gagtagctgt tgtaatgctc ctcggagagg gtgttgaatc agcaataacc agcggtaatg
      961 tcctgctttc ttcgccattg gggctgaaaa tggtggaggg agctggaagc cttgcccgtt
     1021 ttgttgctgg attttttggc taaaataatc tgctaattgc tcaagcagct gtaaggtttg
     1081 ttgattatct ttgccggtcg ctttgaacaa aacttgcgag gaaaatggtg gcaaactcat
     1141 taattgcctc atttttaacg cctcatcagc aaaagctcgg tagccttgtt ccagcaaggt
     1201 tttaagcagt gggtgatcag ggtaatgggt ttgtagcacc acttcgcctt ttttctccgc
     1261 tcgccctgct ctgcctgcca cctgtatgta aagctgtgct aatttttctt ccgcacgaaa
     1321 atcagttgaa aataacgctg aatccacatt aacaatcgcc accaacgtca cattcgggaa
     1381 atgatgccct tttgccaaca tttgcgtacc aatcaaaatt tggcttttgc cctctcgaat
     1441 agcggtcaaa tggttttcca atgcaccttt gcgagcggta gaatctctgt ctatacgagt
     1501 cacttggtaa tgagggaatt gctgatttag cacctgctca agttgttctg tgccgacacc
     1561 ggtagttatt aaatgggttg aaccacaatg tccgcattgg cggggaatta ccctttgggt
     1621 ggcacaatga tggcaacgca acactttttg cttttggtgg taagtaaaag gcttattaca
     1681 cgcctcgcac tcacaaaccc aaccgcattc gtgacaaagt aataccgggg caaagccacg
     1741 gcgatttaaa aacagcatca cttgattgcc ttgctctaac tgggttttca tcattgaaag
     1801 caagcgctcc gacaaaccgg cagttacccg ttgcgttttt aaatcaataa tctgctgatt
     1861 ggcaagttgt gcattgccgg cccgagcggt tagtgtcagt tcgataaatt tcccgttttg
     1921 cacattgtgc aaactttcaa gacttggcgt tgccgagccc agcacaatcg gaatatttgc
     1981 atttttcgcc ctaaccaccg ccaaatcccg tgcgtggtaa cgccaaccgt cttgttgctt
     2041 aaacgagcta tcgtgttctt catccaagat aatcacgccc aatttttgaa actgggtaaa
     2101 taaagcagag cgagtgccga ttacaatcgc actttctcca tttttcgccc gcaaccacgc
     2161 atttaaccgc tcagtttcgt tcatattcga gtgcaatacg tcaatttcaa cgttaaagcg
     2221 agctttaaag cgttgtaccg tttgcggtgt tagcccgatt tcaggcacaa gcagcagcac
     2281 ttgttcctct cgcttaagca ccgtttcaat taactgcaaa tacacttcgg ttttgcccga
     2341 gcctgttacg ccattgagta aaaagctcgc aaaagtggtt tgtgcattta aacggctgac
     2401 cactaaggtt tgttgtttat ttaagatcag ccgattgtta gtatttacta acggaaattc
     2461 acctagtgag tcttgccacg cttgtgccat aaacggcaca tcaattttct cgatataccc
     2521 tttttcgagt aagcctttcc aagcagatgg gctacaagcg gtcggttttt caaaatattt
     2581 tgcacattcg gcaagctcgg ttaaccgttc cacctgcttt ttggcttttt tattttcacc
     2641 gataatcaga gatttccgcc cttgctccgt caccacaaaa taattcggtt gaactcgctc
     2701 agtggaatcg ccattgcgta attttatcgg taaggctccg gttagcactt caccaatcgg
     2761 ggcgtggtaa taacgagccg cccagttcag cagttgccac atttctgagt caaaaatcgg
     2821 gctagaatcc aacgctgctt taatcggttt gagtttttcg gttggcacat cactagtttg
     2881 cgggaaatcg accacaatgc cgattttggt ctggcgacca aaaggcacaa ccacccgccc
     2941 gccttggcag gcattgaatg ccggcggtag caaataatca aaataacgat gtaacggcac
     3001 attcagtgcc actctgatta atctcacttc tctaaaccaa ttttgattgc aagcccgata
     3061 aatatcgagc cgcagatttt atttaaccaa tttccaaagg tttgattatg ccgtaaaaaa
     3121 cgcccaacag gtgcagcggc taagactaaa atacttaccc aaataatgct ggtggataaa
     3181 aacgttagcc ccaacagcat aaaaggtatt ttccaaatta ctggatacaa actgcggcaa
     3241 taacgccaag aaaaagagca atactttcgg gtttaataga ttggtaatca acccttgacg
     3301 gaatatcttc catagaggaa gtttgtctag attgattttc tctaacacca acggctctcg
     3361 ccacatttta atgccgagat agcagagata aattgcccct gcgtatttca gaaaattaaa
     3421 taaagtgggc gaatgtgcca caatgcctgc aagcccgagt gaaaccgcaa gaatatggca
     3481 taaaatgcct gctagaatgc cgaatgaaga cattaagccc gactttttcc cctcagcaat
     3541 agtgcgagtt aaaatataga tagtatccga acccggtgta agatttagca gaatacaagc
     3601 ggtcaaaaat ccccaataat ttacaatatc actcattatt cacactcaat ttacgtttac
     3661 gctgctgacg aagagcaaac ataccatcta atactaaaaa tgaaatggag atcaacaagc
     3721 aaatcatcaa caagtaagat tcatttgcta acctttcgcc aattaagaaa gacaccacca
     3781 acatcaatag tggctcaaca tagcctaata aaccaagcaa attaaagggt aaaatcgtgc
     3841 tggctagcgt atagctgata agtgcagtgc cactaattaa acccagtaat gcaataaaat
     3901 agtaaatatt gaggttttgt gtttcgacat attgcatatc ggtttgagag ataaaataac
     3961 aagctacagg gagcaaatat aaaatttcta ccacaaagct atgtaaatgg cttaagccaa
     4021 actttcggcg aaggtaaaaa tagattgggt aacctgtgca aacaaataaa cttgctatcg
     4081 aaatttttcc cgtaaacaaa atattactcg ctacaccaat gaaagcaacc acaatcgcca
     4141 accatttatt caaggaaagc gtttctttat aaaccacttt gccaaatgcc accataataa
     4201 tcggcattaa tagatagccc attgaaacct caatcgcttt tccgctattg ggagcccata
     4261 aaaacagcca catctgcccg cccacaatag atgccgtgat taaaataacc agcaataaat
     4321 aaggctcttg ctttaggcgt tgaagaaata cccaaaactc attttgtttt ttaaataagt
     4381 ataaagcaag gaaaagaaaa ggaatcgtaa ccagcatacg aatgccgaaa atgctcacac
     4441 ctgccagcgg ttttagaaag atcgccaaat agtacataca gccgaataac actgaggctg
     4501 aaagagataa aagaatgcct tttaacatag tgataagaaa gtaattattt tttaattttt
     4561 caaatctccc ctacccctct tttctaaaga ggattgtcga gttctaaata aatgaataat
     4621 gttcggaggt aggggcgggt ccagtgcccg cccgcaaatt aatatcgtta gcggacaggc
     4681 acaggacctg tccctactga tattatagaa cttacaaccc tgcttttata aagtggggta
     4741 aagggagatt cgacttagtc ttgacgatag gttttcaaga aattcgcaag gcgaccaatc
     4801 gcttcttcaa tttgatgtgc ataaggcaaa gttaccacac ggaagtgatc cggttttttc
     4861 cagttaaaac cggaaccttg cactaatagc actttttctt gtcgcaacaa atcgtagatg
     4921 aatttttggt catctttaat gccgtacatt tcggtatcaa ttttcgggaa catatataac
     4981 gcccctttcg cttttacaca gctaataccc ggaatttgta ccaataattc gtgcatttta
     5041 tttcgttgtt ccagcaaacg accgcccggc aacacaaatt cattgatact ttgataacca
     5101 cctaatgcgg tttgaatggc gtgttgcatc ggcgtattgg cacataaacg catagaagag
     5161 agcatatcca acccttcaat aaaacctttg gcttgatgct tcggaccgct cagcaccatc
     5221 cagccctgac ggaaaccggc aacacgatac gccttggata aaccgttata agttacggtt
     5281 aatacgtccg gtgcaagagc agcaatatgg tgatgtaccg caccatcata gacaattttt
     5341 tcataaattt cgtcagcaaa aataattaag ttatgctgac gggcaagttc tgcaatttcc
     5401 agcaaaattt gacggctata cactgctccg gtcgggttat tcgggttgat aaccaaaatc
     5461 cctttagtgc gaggcgtgat tttcgattta atatcttcaa gatcagggaa ccactcattt
     5521 tcttcatcgc acaggtaatg cactgcttta ccaccggcta gggttgaagc tgctgtccat
     5581 aacggataat caggcattgg aattagaatt tcatcgccat catttaataa cgcctgcatc
     5641 gacattgtaa tcagttcaga tacgccgttg ccgatgtaaa catcgtttac atccatacca
     5701 cgcatacctt ttgattgata atattgcaca atcgctttgc gggcagaata caaacctttt
     5761 gaatcacaat agccttgtgc ggttggaaga ttacgaatca catccactaa aatttcatcg
     5821 ggtgcttcaa agccaaaagg ggctgggtta ccgatattta attttaaaat tttatgccct
     5881 tcttcttcta agcgaagtgc ctctttatga attggcccac ggatatcgta acgaacctga
     5941 gctaacttat ctgatttggg aaaacgatcc attgtaaact tcctataatg tattatctaa
     6001 ttgtagtctc atcccgctgt tcaaaaaatc ggtaattcgg aatgaatttg taaacattac
     6061 tctaatttgg gaggaaaaaa aagaggaaat acgttaaaat ggagaccttt ttttcaatca
     6121 gctaaaaaca agcggtttct ttttctgatt tttttgcaaa tgcttatttt tcatcaatta
     6181 aaacaagaac ttacaaaaag ttactcaaaa gtgggaagag agcaaggatt ggtcgccatt
     6241 caagcctcta tgccgctatt ggaggcaaat attccgctac tcggttggtt gaaaacacaa
     6301 aaaacaagct accctcactt ttttctgcaa tatcgtgata cagatcagac cattgccacc
     6361 attggcacac ttcgccaatt ttatacgcta gaagctgcac aatcttttat tcaacaacat
     6421 catttcccgt taatcggcgg gcttcaattt gaaggcaata cgcaatttat tttgcctcaa
     6481 ttttcattgg taaaaaatca gcaaaattta accgcttgct tctattttga tagtgataac
     6541 tttgaacaac aagcggttat ttttgaacaa tttcttgcaa atttcagcca aattaccgaa
     6601 ttaggactaa cggataatcc gctcgtttca atgagtgcca ccagccattt tgagcaatgg
     6661 aaagccaata ttgaaaaagc gattgtttct attgagcagg gcgattttca aaaagtggta
     6721 ttggcaaatg cgatgagact tcattttgag caacccattt gtgcttacag cttgctggca
     6781 caaagccaaa aaacaaattt aggttgttac cactttttat gggcggaaag tgctgatgtt
     6841 gcctttcttg gctcaacccc tgagcggcta tatcaacgcc aagggctaca cttttctacc
     6901 gaagccctcg ccggcaccgc tgcggttacc accagtgaag tgcaaaccga actcaatgca
     6961 caatggctgt taagtgatcc caaaaatatc tatgaaaatc aattagtggt tgatgacatt
     7021 gaaaaccaac tcaaagattg cgtgaccgaa tttgcagtat ccgaggcaaa aattaagcgt
     7081 ttgcataatg tgcaacacct acgccgccaa attcaagcaa cgctcaaacc ggacacctca
     7141 gattcagatt gcttaagcaa aatccaccca actgcggcag ttgcagggct tccccgccaa
     7201 aaagcaaagg gttttattac cgaaaatgag ccttttaccc gccattggta tgcaggtaca
     7261 ttaggcgtaa tgggcaacga ggaagccgaa ttttgcgtaa cactccgttc tgcattaatt
     7321 tcacaaaatt caattacgct ttatgccggt gccggcattg tgtcaggctc agatccacaa
     7381 tcggaatggc aagaaattaa acgtaaggca caaggaatgg caaaactgtt ggatgttgat
     7441 ttagtaacaa aaggggaact gtaagctagg ctttcagaac ttaatctctt aattatttct
     7501 ttctatattt tcatttccaa cttcccaaca aaatatatta ttgccaccaa attagtagtg
     7561 aatattccat aattaaaaaa tt
//
LOCUS       DANLUQ010000122         7517 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.123, whole genome shotgun sequence.
ACCESSION   DANLUQ010000122 DANLUQ010000000
VERSION     DANLUQ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7517
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.123"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(79..873)
                     /locus_tag="PXB27_001964"
     CDS             complement(79..873)
                     /locus_tag="PXB27_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5346722.1"
                     /translation="MALLEVNHLNKRFVERSSLFHKQDFYAVKEVSFTLNRKETLAII
                     GENGAGKSTLAKMVAGMVGPTSGELIFRGTPLSFGDYKYRAKHIRMMFQDPNDAFDPN
                     YNIGQILDAPLRLATDLNEEERNERIFNTLKLVGMYPEQALVPISEASSGQKQRIAFA
                     RALILEPEIIIADDTINSLDFSVKTQLINLMLSVQQRQGIAYIYVGQNLGLIKHIADK
                     IMVMQSGEVVEYGLTKEVLLNPQHQITKRLIESHFGKHLSSYAWAS"
     gene            complement(974..2050)
                     /gene="fbaA"
                     /locus_tag="PXB27_001965"
     CDS             complement(974..2050)
                     /gene="fbaA"
                     /locus_tag="PXB27_001965"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014990940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HDL5346723.1"
                     /translation="MSLLNIVKPGVVTGDDVQKVFAYAKEHNFAIPAVNCVGSDSVNA
                     VLETAARVKSPVIVQFSNGGAQFYAGKGIKPASGARADVLGAIAGAKHVHQLAEEYGV
                     PVILHTDHAAKNLLPWIDGLLEAGEKHFAETGRPLFSSHMIDLSEEPIEENMEICREY
                     LARMDKIGMTLEIEIGVTGGEEDGVDNSDVDESKLYTQPSEVLYVYDQLSPISPRFTI
                     AAAFGNVHGVYKPGNVKLKPSILGASQEYVSKERGLPAKSLDFVFHGGSGSSREEIRE
                     AISYGAIKMNIDTDTQWAAWDGILQFYKANEAYLQGQLGNPTGPDAPNKKYYDPRVWL
                     RKMEESMSKRLEQSFEDLNCVNVL"
     gene            complement(2147..3322)
                     /locus_tag="PXB27_001966"
     CDS             complement(2147..3322)
                     /locus_tag="PXB27_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012072290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDL5346724.1"
                     /translation="MSVIKMTDLDLAGKRLFIRADLNVPVKDGKVTSDARIVATIPTL
                     KLALQKGAKVMVTSHLGRPTEGVFEEENSLQPVVDYLNASDLGVPVRLVRDYLDGVEV
                     NENEIVVLENVRINKGEKKNDPELAKKYASLCDVFVMDAFGTAHRAEGSTYGVAEYAP
                     VACAGPLLAAELDALGKALKEPQRPMLAIVGGSKVSTKLTVLDSLSKIADQLIVGGGI
                     ANTFIAAEGHAVGKSLYEEDLIPEAKRLAQVTNIPVPVDVRVGTEFSETAPATEKAVS
                     EVRADESIFDIGEKSSEQLAEIIRNSKTILWNGPVGVFEFPNFRKGTEVISNAIAEAT
                     ANGAFSIAGGGDTLAAIDLFGIKDKISYISTGGGAFLEFVEGKVLPAVEILEKRANG"
     gene            complement(3577..4935)
                     /locus_tag="PXB27_001967"
     CDS             complement(3577..4935)
                     /locus_tag="PXB27_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HDL5346725.1"
                     /translation="MANQQQERQSWSNRLAYIMTVAGATVGFGATWRFPYLVGEHGGG
                     AYVFLFCIAMIVIGIPMILVENVIGRRMRVNAIDAFAGFANGKKIASGWKILGYMGLL
                     GAFGILAYYMVLGGWVLNYIGSLLTGTLDLSQPVTVGTTQQFFQQSIFNSPMTIIAYT
                     ALFVLVNYFILVKGIVDGIERSVKYLMPILFIFLIVMVVRNITLPGAAEGIAFYLMPD
                     FSKITPKLFVFVLGQVFFALSLGFGVLITLSSYLDKSENLVKTATITGVMNTLIAVLA
                     GFMIFPSLFSFGVAPNSGPTLVFQSLPIVFSNMWGGTVFAVIFFSLLVVAALTTSLTI
                     YEVIITALQEKAKISRQKAIFITLATIFLLGNIPSALSDNILQDVQVMGKSIFDAFDY
                     ISGNILFILTALGSAIFVGFVLKEEAKEELGAGKMFTTIWFNYVRFVIPVIILVIFFN
                     SL"
     gene            complement(5005..5832)
                     /locus_tag="PXB27_001968"
     CDS             complement(5005..5832)
                     /locus_tag="PXB27_001968"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HDL5346726.1"
                     /translation="MNHPKHRAFLKWAGGKYRLIPDIQTHLPKKACLVEPFVGAGSVF
                     LNTDFERYILADINPDLINLFNVVKADVEEYIAQTKQLFLHPQANTEAFYKARRAEFN
                     RSKDIFRRSVIFLYLNRFGYNGLCRYNQKKGYNVPFGRYKTHYFPEKELRFFAEKAQK
                     ATFVCADFEQVFELAKNQLTDYVIYCDPPYAPLAQETNFTQYAGGGFSLEQQGRLATL
                     ALEMKGLGVPVLISNHDTDFTRRIYQSADITQIQVQRSIGQKVGSRVKVGELFALFI"
     gene            complement(5846..6934)
                     /gene="aroB"
                     /locus_tag="PXB27_001969"
     CDS             complement(5846..6934)
                     /gene="aroB"
                     /locus_tag="PXB27_001969"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDL5346727.1"
                     /translation="MLQVNVELKERRYPIIIGAGLLNQPASYSPLKSGDKVMIVSNPT
                     VATHYLSVVTNALKELGCHVDSVLIPDGEEYKTLESLNLIFTALLEKNHNRDTTLIAL
                     GGGVIGDVAGYAAASYQRGIRFIQIPTTLLAQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     VSVIVDTNTLHTLSKREVSAGLAEVIKYGAIFDVTFFEWLEKHIDDLVSLKQDELEYC
                     IQRCCQLKADVVARDETEKGDRALLNLGHTFGHAIEARMGYGVWLHGEAVSVGMLEAA
                     ELSRILGDLSPQEVARLEKLLARANLPTISPDGMEPAEYLPYMWRDKKVLAGQLRLIL
                     LKSLGEAYVSSQATESQVLASIERFTQR"
     gene            complement(6958..7479)
                     /gene="aroK"
                     /locus_tag="PXB27_001970"
     CDS             complement(6958..7479)
                     /gene="aroK"
                     /locus_tag="PXB27_001970"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005710312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HDL5346728.1"
                     /translation="MAEKRNIFLIGPMGAGKSTIGRQLAQTLGMEFMDSDSVIEERAG
                     ADIDWIFDVEGEEGFRKREERILNELTQNYGIVLSTGGGSILSKDNRNVLSARGIVIY
                     LETTVDKQFERTQRDKKRPLLQTEDPRKTLEELAKIRNPLYEEIADITIHTDEQSAKV
                     VANQIIDMIDNLQ"
BASE COUNT         2331 a         1670 c         1300 g         2216 t
ORIGIN      
        1 attgaaaaga gttattctgc atatttattg aaataggtca aaattatcaa gtaatcatat
       61 tagagcagga gtaaagattt agcttgccca agcataggaa ctaaggtgct ttccaaaatg
      121 actttcaatt aaacgtttcg taatttgatg ttgcggattc agtaaaacct ctttcgtcaa
      181 gccatactct actacttcgc cactttgcat caccattatt ttatcggcaa tatgtttgat
      241 cagacctaaa ttttgcccca cataaatata agcaatccct tgtcgctgct ggacacttaa
      301 cattaaatta attaattgtg ttttgactga aaaatccagt gaattaatcg tatcatccgc
      361 aataataatt tccggttcta aaattaaagc acgagcaaac gcaatacgct gcttttgccc
      421 actggaagcc tctgaaatag gaacgagcgc ttgctcagga tacattccta ccaattttag
      481 cgtattaaaa atacgctcat ttctttcctc ttcatttaaa tctgtcgcca agcggagcgg
      541 agcatctaaa atttgtccta tattataatt aggatcaaag gcatcgttag ggtcctgaaa
      601 catcatacga atatgcttag ctcggtattt ataatcgcca aatgaaagcg gtgttcctct
      661 aaaaatcagt tctccagatg ttggcccaac cataccagca accattttag ccaaggtaga
      721 tttcccagct ccgttttcac caataatcgc taaagtttcc ttgcgattta gggtaaaaga
      781 aacttctttt accgcataaa aatcttgttt atgaaatagg ctactacgtt caacaaaacg
      841 cttattcaga tgattaactt caagtaatgc cataataaat tggtgcttgg aaataaagtg
      901 atagtttacc gcaaaaaata aataaaaaaa gaccgcttat acaagcggcc tgatttatgg
      961 aattttttgc aaattataaa acgttcacac agtttaagtc ttcgaaagat tgctctaagc
     1021 gtttagacat agactcttcc attttacgta accaaacacg tggatcgtag tattttttgt
     1081 ttggtgcatc agggcctgtc gggttaccta attgaccttg taaatatgct tcgtttgctt
     1141 tgtagaattg aagaataccg tcccacgcag cccattgggt gtcagtgtca atgttcattt
     1201 tgattgcacc gtagctgatt gcttcacgaa tctcttcacg tgaagaacca gaaccgccgt
     1261 ggaaaacgaa gtctaatgac tttgccggta aaccacgctc tttagataca tactcttgtg
     1321 aagcacctaa gattgatggt tttaatttta cattacccgg cttgtaaaca ccgtgtacgt
     1381 taccaaatgc tgccgcaatc gtgaaacgtg ggctgattgg gcttaattgg tcgtaaacat
     1441 ataaaacttc agaaggttgg gtgtaaagtt tagattcatc tacatcagaa ttatctacgc
     1501 catcttcttc accaccggtt acgccgattt cgatttcaag ggtcataccg attttgtcca
     1561 tacgggctaa atattcacgg cagatttcca tattttcttc gattggctct tcagataaat
     1621 cgatcatatg tgaagagaat aacggacgac cggtttctgc gaagtgtttt tcgcctgctt
     1681 caagtaagcc atcgatccaa ggtaataagt ttttcgcagc gtggtcggtg tgaagaatca
     1741 caggcacacc atattcttct gctaattgat gaacgtgttt tgcacccgca atcgccccta
     1801 atacatctgc acgagcgcct gaagccggct taatgccttt acccgcatag aactgcgcac
     1861 caccatttga gaattgcacg attaccggtg attttacacg agcggctgtt tctaatacgg
     1921 cattaactga atctgaaccc acgcagttta ctgccggaat agcgaagttg tgttctttag
     1981 cataagcgaa aactttttgt acgtcgtcgc ctgttactac acccggtttt acgatgttta
     2041 ataatgacat ttttttcttc ctttgtatga aatgtgcaaa agcacattgt taaaatatac
     2101 ggccaaatct tgcaaaattt tcacaaaatt tgaccgcttg taactgttag ccgttagctc
     2161 gtttttctaa gatttccact gccggtaata ctttaccttc cacgaattct aagaacgcac
     2221 cgccaccggt tgaaatgtaa gaaattttgt ctttgatacc gaataaatca atcgcagcta
     2281 aggtatcgcc accaccggca attgagaatg caccgtttgc cgttgcttct gcaatcgcat
     2341 ttgaaatcac ttctgttcct ttacggaagt tagggaactc aaacacgccg accggaccat
     2401 tccaaaggat ggtttttgaa ttacggataa tttcagctaa ttgctctgac gatttttcac
     2461 caatatcgaa aattgattca tcagctcgta cttcactcac cgctttttcc gttgccggtg
     2521 cagtttcaga gaattcagta cctacacgca catcaaccgg aacaggaata ttggttactt
     2581 gtgctaaacg ttttgcttca gggattaaat cttcttcgta taatgattta cccacagcgt
     2641 gaccttctgc ggcaataaag gtattggcaa taccaccgcc cacgattaat tggtcggcaa
     2701 tttttgaaag cgaatcaagc acagttaatt tagtagatac tttagaacca cccacaatcg
     2761 ctaacattgg acgttgtggc tcttttaatg ctttgcctaa tgcatctaat tcagcggcta
     2821 ataacggacc tgcacacgca acaggggcat attctgccac accgtaagtt gaaccttccg
     2881 cacggtgagc tgtaccgaat gcgtccatta caaacacatc acaaagtgat gcatattttt
     2941 ttgctaactc agggtcgttt ttcttctcac ctttgttaat acgcacgttt tcaagcacca
     3001 cgatttcatt ctcgtttact tcaacaccgt ctaagtaatc acgcactaaa cgaacaggaa
     3061 cacctaaatc tgatgcattt aagtaatcaa ctacaggttg taaagagttt tcctcttcaa
     3121 atacgccttc tgtcggacga cctaaatgcg aagttaccat tactttagca cctttttgta
     3181 acgctaattt taaggttgga atggtagcaa caatacgggc atctgatgtt actttgccat
     3241 cttttaccgg tacgttaagg tcagcacgaa taaataaacg tttacccgct aaatcaaggt
     3301 cagtcatttt aataacagac atagtttgtt cctctttaga ttaaaattta aaagtgataa
     3361 cggtcggatt atatcagttt ctaatcattt tgggctaacc tagatcaaat aaatgattaa
     3421 ttttttatca ttttagaatg ctattatcct gactgttctt attccccccc aatatgcttg
     3481 gttaattaag caagaattgc aaattcccca caaaatttaa ccgcttgtca ctaaaaataa
     3541 agctgtttga aaatccattc aaacagctta tagaatttag agtgaattaa agaaaatcac
     3601 caagataatc accggaatca caaagcgaac atagttaaac caaatagtgg taaacatttt
     3661 acccgcacct aattcctcct tcgcctcttc ttttagaaca aagccaacga aaatcgcgct
     3721 acctaaggcg gttaaaataa acagaatatt gccactaata taatcgaagg catcaaaaat
     3781 gctcttgccc ataacctgca catcttgcaa tatattgtca cttaatgctg atggaatatt
     3841 acccaacaga aaaatagtag ccaaagtaat aaaaatagct ttttgacggc taatctttgc
     3901 cttctcttgc aatgcagtga taataacttc ataaatagtt aacgaggttg ttaatgccgc
     3961 caccactaat agactaaaaa agataaccgc aaaaactgtt cccccccaca tatttgagaa
     4021 cacaatcggc aaactttgga atactaaagt cggacctgaa tttggagcaa cgccaaagct
     4081 aaataatgac ggaaaaatca taaaaccggc aagtaccgca attaaggtgt tcatcacccc
     4141 agtaattgtt gctgttttta ctaaattctc gctcttatca aggtagcttg aaagggttat
     4201 caacacacca aaaccaaggc ttaatgcaaa aaatacttga cccagcacaa acacaaacaa
     4261 ttttggtgtg atttttgaaa aatcgggcat cagataaaag gcaatgcctt cagcagcccc
     4321 cggtaaggta atatttcgaa ccaccatcac aatcaggaaa ataaacagaa tcggcatcaa
     4381 atatttcact gaacgctcaa taccgtccac aatgcctttt accaaaataa aataattcac
     4441 caatacaaaa agtgccgtat aagcaataat tgtcattggg ctattaaaaa tactttgttg
     4501 gaagaattgt tgggtcgtgc caacggtaac aggttgagac agatctagtg tccccgtgag
     4561 taagctaccg atataattta atacccaacc accaagtacc atatagtagg caagaatacc
     4621 aaatgctcca agcaagccca tatagccaag aattttccaa cctgaggcaa tttttttacc
     4681 attagcaaaa ccggcaaaag catcaatagc attcactctc attcggcgac caattacatt
     4741 ctccactaat atcataggga taccaattac gatcatcgca atacaaaata aaaacacata
     4801 agcaccgcct ccgtgttccc ccacaagata agggaaacgc caagttgcac caaaaccgac
     4861 tgttgcacca gcaacggtca taatatacgc caatcggtta gaccaagatt ggcgttcttg
     4921 ttgttgattt gccatttaat actccatcgg ctaacaatac cgcttcaacc aacaagcggt
     4981 cagattttta gaaaatatta caaattaaat gaagagtgcg aataactcgc caactttaac
     5041 ccttgagccc accttctgcc ctattgaacg ctgtacctga atttgggtaa tatcggcaga
     5101 ttgataaatc ctacgggtaa aatcagtatc gtgatttgaa attaatacgg gaacgcctaa
     5161 tcctttcatt tctaaagcca gtgttgccaa acgcccttgt tgctctaggc taaaaccacc
     5221 gccagcatat tgcgtaaaat tcgtttcttg tgctaaagga gcgtaaggtg ggtcgcagta
     5281 aatcacataa tccgttaatt gattttttgc caactcaaag acctgttcaa aatccgcaca
     5341 cacaaaagtt gccttttgtg ctttttcagc aaaaaaacgt agttcttttt ctggaaaata
     5401 atgggtttta taacgcccaa agggaacatt gtaccctttt ttctggttat aacggcaaag
     5461 tccgttatag ccaaatcgat ttaaatacaa aaaaatcacc gagcgacgaa aaatatcttt
     5521 tgagcgatta aactccgcac ggcgtgcttt ataaaaggct tctgtattcg cttgtgggtg
     5581 aagaaacagt tgctttgttt gcgcaatata ttcctcaaca tctgctttca ccacgttgaa
     5641 aagattaatc aagtcaggat taatatcagc aagaatataa cgttcaaaat ctgtattcag
     5701 aaagaccgaa cctgcaccaa caaaaggctc gactaaacaa gccttttttg gcaaatgggt
     5761 ttgaatatct ggaattaagc gatatttacc gccagcccat tttaaaaagg ctcgatgctt
     5821 agggtgattc atttcatttt aaacattaac gttgggtaaa acgctcaata gaggctaata
     5881 cttgagattc tgtggcttgc gatgaaacgt aagcttcacc aagtgatttc aataaaatta
     5941 ggcgtaattg acctgccagt acttttttat ctcgccacat ataaggtaga tattctgccg
     6001 gttccattcc atcaggtgaa attgttggca aattggctcg ggccaataat ttttccaagc
     6061 gagcaacctc ttgaggactt aaatccccca gaatacgaga taattccgca gcctctagca
     6121 taccaaccga aacagcctct ccgtgtaacc atacaccata gcccatacgt gcttcaatgg
     6181 cgtgaccaaa ggtatgtcct aagtttagta atgcacgatc tcctttttcc gtttcatctc
     6241 gagcaaccac gtcagctttt agctggcaac aacgctgaat acaatattcc agctcatctt
     6301 gcttgagact gaccaaatca tcaatatgct tttcaagcca ttcaaaaaaa gtaacatcaa
     6361 agattgcacc gtatttgatg acttccgcca aaccagcact gacttctctt ttggataagg
     6421 tatggagcgt attggtatca acaatcacag aaacaggctg ataaaaagcc ccaatcatat
     6481 ttttaccgag tgggtggttt actgccgttt tcccgcctac tgaagaatca acttgagcca
     6541 gtaaagtggt cggaatttgt ataaaacgaa taccacgttg gtaagaggca gctgcatagc
     6601 ctgcaacatc accgattaca ccaccgccta aagcaattaa agtggtatcg cgattatgat
     6661 ttttttctaa taatgcggta aaaatcaaat ttaatgattc tagcgtttta tattcttcac
     6721 cgtcgggaat caataccgaa tcaacgtggc aacccagctc ctttaacgca tttgttacca
     6781 cactaagata atgtgtcgcc accgttggat ttgagacaat catcacctta tctccagatt
     6841 ttaatgggct ataactagca ggctgattta ataaccctgc accaattata atcgggtaac
     6901 gacgctcttt taattccaca ttgacttgta acatatctta gctcctttct tacctggcta
     6961 ctgtaaatta tcaatcatat caataatttg atttgccact actttagcgc tctgctcgtc
     7021 cgtatgaata gtaatatctg caatttcttc atacaatggg ttacggattt tcgctaactc
     7081 ttccaacgtt ttacgggggt cttctgtttg caataaaggg cgttttttat ctcgctgagt
     7141 acgttcaaac tgtttatcca ctgtcgtttc taaataaata acgataccac gagccgataa
     7201 cacattacgg ttatctttag ataaaatcga accaccgcca gttgaaagca cgataccata
     7261 attttgcgtc agttcattta gaatacgttc ttcacgctta cggaaaccct cttcaccttc
     7321 aacatcgaag atccaatcaa tatccgcacc tgctcgttct tcgatcacac tgtcggaatc
     7381 cataaattcc atacctaatg tttgagcaag ctgacgacca atcgtacttt tacctgctcc
     7441 catcggccca atcaaaaaaa tattacgttt ttcagccatt atttcttctt cattttaaaa
     7501 tttactaaac atttgat
//
LOCUS       DANLUQ010000123         7498 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.124, whole genome shotgun sequence.
ACCESSION   DANLUQ010000123 DANLUQ010000000
VERSION     DANLUQ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7498
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.124"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            172..840
                     /locus_tag="PXB27_001971"
     CDS             172..840
                     /locus_tag="PXB27_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5346729.1"
                     /translation="MAHNGSGKSTLMRLLSGDITPTSGSILVDGLPLHQLSTKEAAKH
                     IAYLPQRLPEAADFLVQELVALGRFPYQKWLQKPTEFDVQIVEQAMESTSVTQFAKQP
                     VNSLSGGEKQRVWLAMCLAQQTPYLLLDEPLAALDIVYQVEVMQLIRKLVNEQGLTVI
                     IIIHDINLASQFCDEIIALKQGELCIQGSVDEIMQEQKLLEIFGLKLHLFNHPAGLHK
                     VAVL"
     gene            837..1718
                     /locus_tag="PXB27_001972"
     CDS             837..1718
                     /locus_tag="PXB27_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL5346730.1"
                     /translation="MMFRQYKRFIFLFILQISTAFAQPNIFTPDWSIASALTEMGNPP
                     IAMGDKRIYPLWSHRPIQPNSVLDIGARYQPNRELLAQLPVELVLSNFFYAHLASVYP
                     KEIPQIDVLFDGGNTEPQQHWHTYQKATRMIGTEIKNPNAAEAYLTKTEQTLAQWGKE
                     ILNSNPDIDYYSVVQFGSPRELRIYASNSMFRVAFDLMGLKQADLGVGDRWGNKLIPV
                     SDLAKLPERTCLIVISPFSKMTQADLENSYIWQRLGFGKNRCMKVLPPVWLFGGADSI
                     SHFAEFLHNAMTQGEKE"
     gene            1718..2533
                     /locus_tag="PXB27_001973"
     CDS             1718..2533
                     /locus_tag="PXB27_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL5346731.1"
                     /translation="MKSSFLLIGCILLSLVTAVIRLNMVWEWEWQLLLINVDLLPINQ
                     AAVKAALLPEMTTSFLAGGLLAIASSGLQHIVRNNLASDSTLAVSSGAQLALMIAVIF
                     FPAAELFSSFWIALLGSLAAIVLVLFIAKASKLNPLTLILGGLIVNILFGAIASLLTL
                     FYFDFLFGVMVWGSGSLLQDGWATSITLAITVVVAFFIFVLLSRPLTILSLDDEQARR
                     LGAPVNLLRYLVIFVCAAITALVVSKIGVIGFIGFAVQVWLILPKYDIYYFAS"
     gene            2530..3666
                     /locus_tag="PXB27_001974"
     CDS             2530..3666
                     /locus_tag="PXB27_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL5346732.1"
                     /translation="MIAFLAGGFLLFFTNNLIGIVSRIYDTLLPVGAFTATLGAPLLI
                     FLVLRQRKAQFEASNTAYFPVKRLNSWKLPILLLCAGVILFCLLQIIAPTMNGWAISI
                     DENLMSSYRLPRSLSAIATGAMLAVSGSLLQILTRNPMASPEVLGVSSGATIAAVLSF
                     LLFPTLSSISFLLFGVLGSLTVLALIMWLSRKLQPSALMITGVAIGALVSGLMAIIQL
                     SGSPRLTAVISWLSGSTYYANPNTVWILLSVAILLISATLLFIKPLRLISLGETVSYH
                     LGLEVKKVQMIVLLLIALMSAASTLAVGPMSFIGLMTPHLAKNLGAITPEKQIPISAL
                     LGAMLMLIADWLGRYLIFPYEIGAGIIASILGGIYFLLLLKQHK"
     gene            3694..4092
                     /locus_tag="PXB27_001975"
     CDS             3694..4092
                     /locus_tag="PXB27_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HDL5346733.1"
                     /translation="MNTLSKAIKLALYSSGIGFISTAYAETVTHLDTVEVSAETPNAS
                     SEETGRYAQKKAKGSNGMALSAKDTPQSVTAVTHQQIRDQNLNTIAKALEATHGVSVS
                     LLDRGRYSFSARGFGIDKVKVDGMDLKVSK"
     gene            4127..5830
                     /locus_tag="PXB27_001976"
     CDS             4127..5830
                     /locus_tag="PXB27_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HDL5346734.1"
                     /translation="MLYDRVEVVRGATGLTTGAGDPSASVNLVRKRANSISRYTILEA
                     GIGRYADFNTMIDHSQALNNSGSVRGRFIAEHKNGNTFIKNEKERYTTLYGNIEADLT
                     DTTKLGLGVSYQKEKRDAALWGGLPAFYSDGGETNWSRSKNASPEWAYWDNESVNYFA
                     DITQKLGEKWELALKGNYRVGKHNSELFYFSGSSLNRQDGLGWSPWPGKFKNDNTQSN
                     VQLDLTGSFSAWGLEHDAAIGIQYNRAHRNSYAADNSTFDGAFDFNNWNGIYARPNWG
                     SMSEKYYQNLKEFGVYVSTRLHLTDRLSMLLGSRISSYRTEGTFYGSTQNFHANDVWT
                     PYAGLTFDINPQNTVYVSYTDIFKPQSQVDINNKLLSPVTGETYEAGWKGSYFNDNLQ
                     TQISVFETRQDNLAQSAGINPNTLLAYYTASKGAKVRGFEVEASGNVTEKLKLSAGYT
                     QWKGDDASGNPINTTQPRKQFKLFATYDMNRLVQGLTVGAGVNWQSRIYTKHATYGEY
                     GQKAYTLVNLMARYQFNDNFSAQLNIDNLFDKKYRNALSFSQYSYGEPFYTRLNLRYE
                     F"
     gene            5942..6586
                     /gene="adk"
                     /locus_tag="PXB27_001977"
     CDS             5942..6586
                     /gene="adk"
                     /locus_tag="PXB27_001977"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005721186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDL5346735.1"
                     /translation="MKVILLGAPGAGKGTQAQFIMNKFGIPQISTGDMFRAAIKESTE
                     LGKQAKALMDEGKLVPDELTVALVKDRIAQPDCANGFLLDGFPRTIPQADALKESGVK
                     IDLVLEFDVADEVIVERMSGRRVHQPSGRTYHVVYNPPKVEGKDDVTGEDLIIRQDDK
                     PETVLERLAIYHKQTKPLIAYYTAEAEAGNTRYERLDGTKPVEEVSAELAKILS"
     gene            complement(6648..7463)
                     /locus_tag="PXB27_001978"
     CDS             complement(6648..7463)
                     /locus_tag="PXB27_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDL5346736.1"
                     /translation="MSYKVVFSLLFSLFLSTANANWTKINDVNYTWGPFNIYHITLFT
                     ESGTYVEGTRPLMLTLQYKKPWEGRDFAVSLARTWNNLGIKLPEKELDEVITHLRKTF
                     PDIKPEDTLHYIALEDRGYFILNDKVVSDVFNKAFNDAIVAIWLDPKMDISHQLLDPS
                     YKPRAEAESIKPQVEERVAENLQEPIVNTEVSKELKADSQAVEQAPNKIDIPVADKTQ
                     ASVVNEATQPVENKDKKEPEKLEEKPKEMKIENPEVFISPIYDDILMLTKYCV"
BASE COUNT         2195 a         1479 c         1481 g         2343 t
ORIGIN      
        1 agattttcat ctactcattt tcattttgat tgatttggtg gggtaatccc acctttttta
       61 tgctcttaaa acacaacact atgattcaaa taaattccat tagctactct gttgaacaaa
      121 accgtaaact gctaagcaat atttccctca atttaagcaa ggggcgttta tatggcgcat
      181 aacggctcgg gtaaatcaac gttaatgcgt ttactaagcg gagatataac gcctaccagc
      241 ggttcaattt tagtcgatgg attaccactt catcagcttt ctaccaaaga agcggcaaaa
      301 catattgcct atttaccaca acgtttacct gaagcggcgg atttcttagt gcaggaactg
      361 gttgctttag ggcgatttcc atatcaaaaa tggctacaaa aaccaacaga atttgatgtt
      421 caaattgttg agcaagcaat ggaaagcacc tccgttactc agtttgctaa acagcctgta
      481 aatagtctat caggcggaga aaaacaacga gtttggcttg ctatgtgctt agcccaacaa
      541 acaccctatt tactgctaga tgagccattg gctgcccttg atattgttta tcaagttgaa
      601 gtaatgcagc ttattcgcaa gctagtgaat gaacaaggtc taactgtcat tatcattatt
      661 cacgatatta atcttgcctc ccaattttgt gatgaaatta ttgcgttaaa gcaaggcgaa
      721 ctctgtattc aaggttctgt tgatgaaatt atgcaagaac agaaattact tgagatcttc
      781 gggctaaaac tgcacttgtt taaccaccct gcaggcttac ataaagtggc ggtattatga
      841 tgtttaggca atacaagcgg ttcatttttc tctttatttt gcaaatttct accgcatttg
      901 ctcaacctaa tatttttaca ccagattggt ctattgcctc tgcccttact gaaatgggaa
      961 atccgcccat tgcgatgggg gataagcgaa tttatccgct atggtcgcac cgccctattc
     1021 agcctaatag cgtgttagat attggagcgc gttatcagcc taatcgcgaa ttattagcac
     1081 agcttcctgt tgaattggtg ctatcaaact ttttctacgc tcacttggct tctgtttacc
     1141 cgaaagaaat tccacaaata gacgtgctat ttgatggtgg caatactgaa cctcagcaac
     1201 attggcatac ttaccaaaaa gccactcgta tgattggaac agagattaaa aaccctaatg
     1261 cggcagaagc atatttaacg aaaactgaac aaacgttggc tcaatggggg aaagaaatcc
     1321 ttaacagtaa tccagatatt gattactact ctgttgttca atttggctct ccgagagagt
     1381 tacgcatata tgcgtctaat tcaatgttcc gtgtagcctt tgatttgatg ggcttaaaac
     1441 aagctgattt aggtgtggga gatcgctggg gaaataaact tattcctgtg agtgatttag
     1501 ctaaattacc cgaacgcacc tgtttaattg tcatctcacc tttttctaaa atgacgcaag
     1561 cagatttaga aaatagctac atttggcaac gtttaggatt tggtaaaaac cgttgtatga
     1621 aagtattacc gcctgtttgg ctctttggtg gggctgattc catctcgcac tttgctgagt
     1681 ttttacacaa tgcaatgaca caaggagaaa aagagtaatg aaatcatcat tcttattgat
     1741 tggctgtatt cttttatcct tagtaacagc tgttatccgt ttaaatatgg tctgggagtg
     1801 ggaatggcag ttactactca taaatgttga tttattgcca attaatcaag ctgcggtaaa
     1861 agcagctctt ttacctgaaa tgacaacgtc atttttagca ggcggtttat tggctatagc
     1921 cagcagtggc ttacaacata ttgtcagaaa taatttagcg tcagatagca cacttgccgt
     1981 cagtagcggg gctcaacttg cattgatgat tgcggttatc tttttcccag ccgccgagct
     2041 tttttctagt ttctggattg cgttacttgg ctctttagct gctatcgtac tggttttatt
     2101 tattgctaaa gcctctaagc tcaatcccct tacccttatt ttagggggat tgattgttaa
     2161 tattttgttt ggagctattg cctctttact gacccttttc tactttgatt ttttatttgg
     2221 cgtgatggtt tggggaagtg gctcactttt acaagatggt tgggcaactt ctattacctt
     2281 agcgataacg gtagtagttg cattttttat ctttgtctta ctctctcgtc cactcacaat
     2341 tttaagttta gatgatgaac aagctcgccg cttaggcgcg cctgttaatt tactacgcta
     2401 cttagttatt tttgtttgtg ccgccattac agcattggtt gtaagcaaaa taggtgtgat
     2461 tggctttatt ggatttgcag tgcaagtttg gctcattttg ccaaaatacg acatttatta
     2521 cttcgcctct tgattgcttt tctagcgggc ggatttttac tcttttttac caataatctc
     2581 atcggcattg ttagcagaat ttacgacact ctactacctg ttggtgcatt taccgcaaca
     2641 ctaggcgcac cattattaat tttcttggta ttacgccaac gtaaagcaca atttgaagca
     2701 agcaatacag catacttccc cgttaaacgc ctcaattcgt ggaaactgcc aatattgttg
     2761 ttatgcgctg gagttatcct attttgtcta ttacaaatca ttgctccaac aatgaatggc
     2821 tgggctatta gcattgatga aaacctgatg agttcctacc gcttgcctcg tagtctaagt
     2881 gcgatagcaa caggggcaat gcttgcggta agtggtagcc tattacaaat cttaacgaga
     2941 aacccaatgg caagccctga agtattaggt gtgagttcag gggcaactat tgctgcagta
     3001 ctgtcattct tacttttccc aacattatct agcattagct tcttgttatt tggtgtatta
     3061 gggagtttga ctgttctcgc tcttattatg tggctctctc gtaaattaca accatcagcg
     3121 ttaatgatta caggtgttgc tattggagct ttagtcagtg gcttaatggc tattattcaa
     3181 ctaagcggca gccctcgttt aacggcagtt attagttggc tgtctggctc aacctattac
     3241 gctaatccga atacagtttg gattttatta tctgttgcaa tcctacttat cagtgccact
     3301 ttactgttta tcaaaccttt acgtttgatc tcacttggcg agaccgtttc ttaccattta
     3361 gggttagagg ttaaaaaagt acaaatgatt gtattacttt taattgccct aatgagtgcc
     3421 gcctctacac ttgcggtagg gccaatgagc ttcattgggc taatgacccc tcatttagct
     3481 aaaaatttag gtgcaattac accggagaaa cagatcccta tatcggcatt attaggggca
     3541 atgctaatgc tcattgccga ttggctcggg cgttatctaa ttttccctta tgagattggg
     3601 gcaggcatta ttgcctctat tctcgggggt atctatttct tactgttact caaacaacat
     3661 aaataatttt ttattcatta cttaggagaa attatgaaca cgctatctaa agcgataaaa
     3721 cttgccttat acagttctgg tatagggttt atctcaacag cttacgctga aacagttact
     3781 catcttgata ccgtagaagt aagtgccgaa acgccaaatg cctcatcgga agaaactgga
     3841 cgttatgcac agaaaaaagc caaaggctct aatggaatgg cattgagtgc gaaagacacg
     3901 ccacaaagcg taacggctgt aacgcatcaa caaatcagag atcaaaatct taatacgatt
     3961 gccaaagcac ttgaagcaac tcacggtgtt tctgtttctt tacttgacag aggacgctat
     4021 tctttcagtg ctcgtggttt tgggattgat aaagtcaagg tagatggtat ggatctcaaa
     4081 gtaagtaaat aaccaatgga caacgggcga aaatacaggc aacagcgtgt tatacgaccg
     4141 agtagaagtt gtgcgtggtg ctacaggctt aactactggg gcaggcgatc cttctgcaag
     4201 tgttaatctt gttcgtaaac gagccaatag catttcccgt tatactattt tagaagctgg
     4261 aatcgggcgt tatgctgatt tcaatacaat gattgaccat agccaagcat tgaataattc
     4321 aggatcagta agaggccgct ttattgcaga gcataaaaac ggtaatacgt tcattaaaaa
     4381 tgaaaaagaa cgttacacca cgctttatgg caatattgaa gcagacttaa ctgacactac
     4441 caaacttggg ttaggtgtaa gctatcaaaa agaaaaacgt gatgcagcac tttggggtgg
     4501 tttacccgca ttctatagcg acggtggtga aactaactgg agcagaagca aaaatgccag
     4561 tccggaatgg gcttactggg ataatgaatc cgtaaactat tttgccgata ttacacaaaa
     4621 actaggcgaa aaatgggaac ttgcattaaa aggcaattac cgtgtaggca aacataattc
     4681 agaacttttc tatttcagcg gctcatcact taatcgccaa gatggattag gttggtcgcc
     4741 ttggccgggt aaatttaaaa atgataatac acaatctaat gtgcagcttg atcttaccgg
     4801 ttcattctct gcttggggat tagaacacga tgcggcaatc ggtattcaat acaatcgagc
     4861 acatcgtaat tcctacgctg ccgataatag cacctttgac ggagctttcg actttaataa
     4921 ttggaatggc atttatgcta gaccaaactg gggaagtatg tctgaaaaat actaccaaaa
     4981 cttaaaagag ttcggggtgt atgtttctac ccgtttacat cttacagata gactttctat
     5041 gcttctaggt tctcgtattt catcgtaccg cacagaaggc actttctatg gttcaacaca
     5101 aaacttccac gctaacgatg tttggacacc ttatgcagga ttaacctttg atattaatcc
     5161 acaaaatacc gtttatgtaa gttataccga tattttcaaa ccacaaagtc aagtggacat
     5221 caataacaaa ctactctctc ctgttacggg cgaaacctac gaagcaggtt ggaaagggag
     5281 ttatttcaat gacaacttgc aaactcaaat tagcgtattt gaaacaagac aagataatct
     5341 agctcaatct gccggtatta accctaatac attattagcc tactacacag cctctaaagg
     5401 tgcgaaagtt cgaggctttg aagtagaagc atctggaaat gtaactgaaa aattaaaact
     5461 cagtgcagga tatactcagt ggaaaggcga tgatgcttca ggtaacccaa ttaacacaac
     5521 tcaaccacgc aaacaattta aactctttgc aacttacgat atgaaccgtt tagtgcaagg
     5581 tttaacggta ggagcaggtg taaactggca aagcagaatt tataccaaac acgctactta
     5641 tggcgaatat ggtcaaaaag cttacacttt agtgaatttg atggctcgtt atcaatttaa
     5701 tgacaacttc tcagcacagc ttaacattga taacttattc gataaaaaat atcgtaacgc
     5761 cctcagcttt agccaatata gctacggtga gccattctat acacgcttaa atttacgcta
     5821 tgaattctaa ttccgtataa caagcggtca aaaaattaaa aaaatttgca attttaggca
     5881 gtaatcggta aggttactgc ctaagtgatt ttattatttt tagtttaatt gaggttttca
     5941 aatgaaagtg attttattag gtgcgccggg tgcgggcaaa ggtacgcaag ctcagtttat
     6001 tatgaacaaa ttcggtattc cgcaaatttc aaccggcgat atgttccgtg cagcgattaa
     6061 agaaagcact gagcttggta aacaagcaaa agcattgatg gacgaaggca agttagtgcc
     6121 ggatgaatta acggtcgcct tagtaaaaga tcgtattgct caaccggatt gtgctaatgg
     6181 cttcttatta gacggtttcc cacgcaccat tccgcaagct gatgcgttaa aagagtcagg
     6241 tgtaaaaatt gatttagtgt tagagtttga tgtagctgat gaagtcattg ttgagcgtat
     6301 gagtggtcgt cgtgttcacc aaccttcagg gcgtacttac cacgtggtgt acaatccacc
     6361 gaaagtggaa ggcaaagatg atgtgactgg tgaggattta attatccgcc aagatgacaa
     6421 acctgaaacg gtattagagc gtttagcgat ttaccacaaa caaaccaaac cgttaatcgc
     6481 ctactacacc gcagaagcag aagccggcaa cactcgttat gagcgtttag atggaacaaa
     6541 accagtggaa gaagtaagtg ctgagttagc gaaaatttta tcttaaatgg attattaaaa
     6601 tgaacaagcg gtaatatttt tcgagaatat taccgcttgt tctttgctta aacacaatat
     6661 ttggtgagca ttaagatatc atcataaatc ggtgaaataa acacttccgg attttcaatc
     6721 ttcatctcct ttggtttttc ttctagcttt tctggctctt tcttatcttt attctctacc
     6781 ggttgagtcg cttcatttac cacactagct tgagttttat ctgctacagg tatatctatt
     6841 ttgtttggtg cttgctctac tgcttgagaa tccgctttca gctctttaga aacttcagta
     6901 tttacgatag gttcttgtaa gttttccgct actctttcct ctacttgtgg cttaatactt
     6961 tctgcctctg ctctcggttt ataagatgga tcaagtaatt gatggctaat atccattttc
     7021 ggatcaagcc aaattgctac aatagcatca ttgaaagctt tattaaacac atcagagact
     7081 actttatcat ttaaaataaa ataacctcta tcttctaatg caatatagtg caatgtatct
     7141 tcaggcttaa tatctgggaa agtttttctc aggtgggtaa taacctcatc taactctttt
     7201 tcaggtagct taatgcctaa attattccaa gttcttgcta aagaaaccgc aaaatcacgc
     7261 ccctcccacg gttttttata ttgtagagta agcattaaag gacgtgtacc ttctacataa
     7321 gtaccgctct cagtaaaaag cgtaatatgg taaatattaa atggccccca cgtataattc
     7381 acatcattaa tttttgtcca atttgcattt gctgttgata aaaatagaga aaacaacagc
     7441 gaaaagacca ctttatagct cattattttt gtgtccttaa atctaaaatt cctactca
//
LOCUS       DANLUQ010000124         7424 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.125, whole genome shotgun sequence.
ACCESSION   DANLUQ010000124 DANLUQ010000000
VERSION     DANLUQ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7424
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.125"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            48..1844
                     /locus_tag="PXB27_001979"
     CDS             48..1844
                     /locus_tag="PXB27_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate-specific subunit IIBC"
                     /protein_id="HDL5346737.1"
                     /translation="MDFLTEILLFLKDQVLNKAPFLLGIVACLGYILLKKDTSTIIKG
                     TIKTIVGFMIVQAGSGFLVTNFKPVIDGLAKYHQLTGAVIDPYTSMQSTIQTMGENYA
                     WIGYAVIGALFLNILLVVFRRLTGIRTIMLTGHIMFQQAGLVAVFYMIIGASMWETII
                     YTSVLMALYWGISSNIMYKPTQAVTGGAGFSIGHQQQIASWIAVKLAPKLGDKDDTVD
                     KMKLPNWLNIFHDSISATALVMTIFFGIILLSFGLDNLKELAGKTNQFMYILEMGLKF
                     AVAIQIIVTGVRMFVAELSEAFKGIAERLIPNSVLAIDCAAIYAFSPNAMVFGFMWGA
                     IGQFVAVGGLLAVGSPVLIIPGFIPMFFSNATIGVFANQFGGWKAVMKICFVMGIIEV
                     LGSAWVIQMLASQGTTFNGWMGMADWALFFPPILQGIISIPGFFFVIVAAAMVYMFFA
                     SKQLRAEEAEAAARGITLEQLDGYGLDEVKAEPKAEAVVQQAVENAENSANPIRILAV
                     CGSGQGSSMMMKMKIKGYLDKRGIPNIMDSCAVTDYKGKLSEVDIIVSSKHLANEIEV
                     PEGKAVLGVQNMLNPNSFGDELVELINQHNAK"
     gene            1924..2985
                     /locus_tag="PXB27_001980"
     CDS             1924..2985
                     /locus_tag="PXB27_001980"
                     /inference="COORDINATES: protein motif:HMM:NF012376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL5346738.1"
                     /translation="MKKILSTLFMLSAVSVAVAKEVNIKLLGTSDVHGRVVPWSYGAD
                     VEDKSGSFAQIATYVKEVRANHKNVLLFDIGDAIQDNQVEEFAKTLDKAKNNPVPKVL
                     NAMNYDIFILGNHEFNFGMPNLDAILKDINAKILTANFYHKDGKRYVEPTTIIERDGV
                     KLGIIGLTTPMSAIFEKDTGHLDAMKFTSPIEEAKAQIADLKAKGVDAIIVLAHMGIE
                     NENNIPDTGVADLVNAVDGIDVIIAGHMHKDVPSETIKNTLITEPHRYGTVVSEVDLA
                     FDVNDKGEVKLLSKTAKTVPVKKLASDPEIEKIYQPYHNELRALNNVKIGETAQDLIP
                     QGKITVYRLLSRKTPECPH"
     gene            2973..3437
                     /locus_tag="PXB27_001981"
     CDS             2973..3437
                     /locus_tag="PXB27_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HDL5346739.1"
                     /translation="MSSLIHDVQQHYSGADVVSFAFDYETVRLDKGDIKKKDIIYNYR
                     YAGGDVSMYELTGKQLKQYMEWSADYFDTIQAGDKDYRYNAVRGKSKYVTFDLFGGVS
                     YNIDLRNPSGSKIVDLKLANGSLITDDGKYKVGMNSYRFGQLTKKGGIWEGQ"
     gene            3468..3584
                     /locus_tag="PXB27_001982"
     CDS             3468..3584
                     /locus_tag="PXB27_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ser/Thr protein phosphatase"
                     /protein_id="HDL5346740.1"
                     /translation="MGAEKGTIQNMLIDYITNVKGGKIEGKSHNRWAIIGLN"
     gene            3600..4067
                     /locus_tag="PXB27_001983"
     CDS             3600..4067
                     /locus_tag="PXB27_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL5346741.1"
                     /translation="MNLKQSLIENNSILLNQSAANWEDAIKIGTDLLVKAGTIEPRYY
                     DNIVSKIKEMGPYIILAPGLAMPHARPEEGVIKTSFALVTFDTPIYFEGEDEPVHVML
                     TLAGSDSDQHMQGLVEITQVLDDPESDTGVDLDKIRRCKTAEEVYAVIDQALA"
     gene            4122..4802
                     /locus_tag="PXB27_001984"
     CDS             4122..4802
                     /locus_tag="PXB27_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDL5346742.1"
                     /translation="MSKPLLQIALDSLSLEKAVADAKQAENQVEIIECGTILAFAEGM
                     KAVSTLRVLHPNHIIVCDLKTTDGGAILAKMAFEAGADWLTVSAAAHPATKAACKKVA
                     DEFNAAHPELKVKKEIQIEIYGNWTVEKDAKEWVELGVTQAIYHRSRDAELAGKGWTT
                     EDVELMKQLSDLGIELSITGGIVPEDIHLFKEIKNAKAFIAGRALVGEKGKQTAEAIR
                     VEIDKYWN"
     gene            complement(4870..6012)
                     /locus_tag="PXB27_001985"
     CDS             complement(4870..6012)
                     /locus_tag="PXB27_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL5346743.1"
                     /translation="MKIVIAPDSFKESLTALEVAQAIQQGFARVFPEAEYQLVPMADG
                     GEGSVQSLVDATHGSLQKISVIAPLANEVEGFFGLSGDQKTAFIEMAAASGLHLVPME
                     QRNPLVTTSYGTGQLIKAALDLGVEKIILGIGGSATNDGGVGMLQALGAKFLNAEQRE
                     IGFGGEALSQIQQIDLSGLDSRLKQVEFEVACDVDNPLCGERGASAIFGPQKGATPEM
                     VKMLDHALLHFAETVKSQLGIEIAEKAGAGAAGGMGGGLLLLPNVTLRSGVQIILEAT
                     DLAEKLADADLVVTGEGRMDAQSIAGKTPIGVAKVAKQFGKPVIAIVGSLKEDYPVVY
                     QHGIDAVFPIIRQVSNLEAVLKSGKENLISTAENVARLWKISALQE"
     gene            complement(6022..7401)
                     /locus_tag="PXB27_001986"
     CDS             complement(6022..7401)
                     /locus_tag="PXB27_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005757929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDL5346744.1"
                     /translation="MSGIALILIFLVAIIIMIYMIAKWKVHPFLALMSISIALALVAG
                     LPLAKIPTIVGEGFSNTFKSIGIVIIFGAIIGTILEKTGAALKLADMVVKVVGQKRPE
                     LAMLIMGWVVGIPVFCDSGFVVLNPIREAIRKKILANPVGMAVALSAGLYASHVFIPP
                     TPGPIAAAGAVGLSHNLLLVIGMGFVVSIPVLIASYFFAKYVGKQVSVMEEAQADQVI
                     SQSYEDLIKQYGKLPSGFLSFAPIFMPIILMAIGSIAKIAGVSGDFGVFLQFVGNPII
                     ALAIGVIFAIFLLVSSGKMAEFDSLTNDTLKLVGPILFITAAGGVLGNVITQAGFVDY
                     IKQNAHIISTAGIFFPFLISAILKTAQGSSTVAIITTASIMGMFNAPDSLMNALGLTS
                     EMAAALTVMAIAAGSMCVSHANDSYYWVVTNFTKLTPQQGYRTQTTLTFIMGVVGMLT
                     VYLLSLILL"
BASE COUNT         2181 a         1725 c         1611 g         1907 t
ORIGIN      
        1 ctctggagtt ttttagttct attaatctct caacaaagag gccccctatg gactttttaa
       61 ccgaaattct ccttttctta aaggatcaag tgctgaataa agcacccttc ttactaggta
      121 tcgtagcttg cttaggttac attcttctta aaaaagacac ttctacgatt attaaaggca
      181 caattaaaac aattgtcggt tttatgattg tacaagccgg ttcaggtttc ttagtaacca
      241 actttaagcc ggttattgat ggcttagcca aatatcacca acttaccggt gcagtaattg
      301 acccttacac cagtatgcaa tcgaccattc aaaccatggg cgagaactac gcttggattg
      361 gttatgccgt aattggggcg ctgttcctca atattctatt agtggtgttc cgccgtttaa
      421 ccggtatccg taccattatg ctcaccggtc acattatgtt ccaacaagca ggcttggttg
      481 cggtgttcta tatgatcatc ggagcgtcaa tgtgggagac cattatctac acctcagtgt
      541 tgatggctct ttattggggt atttcgtcta atattatgta taaaccaacc caagcggtaa
      601 caggcggtgc aggcttctca attggtcacc aacaacaaat tgcttcttgg attgcagtca
      661 aactcgcacc aaaacttggc gataaagacg atactgtaga taaaatgaaa ttgcctaact
      721 ggctcaatat tttccacgac agtatttctg caaccgcatt agtaatgacc atcttctttg
      781 gaatcatctt actttcattc ggtttagata acctcaaaga acttgctggc aaaaccaacc
      841 agtttatgta cattcttgaa atgggcttga aatttgcggt ggcaattcaa atcattgtaa
      901 ccggcgtacg tatgtttgta gctgagcttt cagaagcctt caaaggtatt gctgaacgct
      961 taattccaaa ctcagtatta gcgattgact gtgccgctat ctacgctttc tcgccaaatg
     1021 cgatggtatt cggctttatg tggggggcta tcggtcaatt tgtggcagta ggcggcttgc
     1081 ttgcagtagg ctcaccggtg cttattattc cgggctttat cccgatgttc ttctctaacg
     1141 caaccattgg tgtgtttgca aaccaatttg gtggttggaa agcagtaatg aaaatctgtt
     1201 ttgtgatggg tattattgaa gtgcttggct cagcttgggt tattcaaatg cttgcttcac
     1261 aaggcacaac ctttaacggt tggatgggta tggctgactg ggcattgttc ttcccaccaa
     1321 tcctacaagg tattatcagc attccgggct tcttctttgt aattgtggca gcagcaatgg
     1381 tttatatgtt cttcgcatct aaacaacttc gtgccgaaga agcagaagcg gcagctcgtg
     1441 gcattacact tgaacaatta gacggctacg gcttagatga agtaaaagca gaaccaaaag
     1501 cggaggcagt agtacaacaa gcggtcgaaa atgccgaaaa ttctgcaaac ccaatccgca
     1561 ttctagcggt gtgtggctct ggtcaaggct catcaatgat gatgaaaatg aagatcaaag
     1621 gctatctcga caaacgtggt attccgaata ttatggattc ctgtgcggta actgattaca
     1681 aaggcaaatt aagcgaggtt gatattattg tctccagcaa acatttagcc aacgaaatcg
     1741 aagtaccgga aggcaaagcg gtattaggtg tacaaaatat gctaaatcca aactcatttg
     1801 gtgatgaatt agttgagcta atcaatcagc ataatgcaaa ataagtaagt ttaggggtga
     1861 ataatcaccc ctatctatgc taaagattgt aaaaactcac ttcatcaaac gaggaattat
     1921 ccaatgaaaa aaatcttatc caccctgttt atgctctccg cggttagcgt tgccgttgcc
     1981 aaagaggtaa acattaaact acttggtacc tcagacgttc acggacgtgt tgtgccgtgg
     2041 agctacgggg cagacgtgga agacaaatcc ggctcattcg cccaaattgc aacctatgtc
     2101 aaagaggtgc gtgccaacca caaaaatgtg ttgctgtttg atattggcga tgcgattcaa
     2161 gacaaccaag tcgaagagtt cgctaaaacc ttagacaaag cgaaaaacaa tcccgtgccg
     2221 aaagtgctga atgcaatgaa ttacgacatc ttcattctcg gcaaccacga atttaacttc
     2281 ggaatgccga atttagatgc gattttaaaa gacatcaacg ccaaaattct cactgccaac
     2341 ttctatcaca aagacggcaa acgctacgtt gagccaacta ccatcattga acgtgatggc
     2401 gtaaaacttg ggattatcgg cttaaccacc ccaatgtcgg ctatttttga aaaagacacc
     2461 ggtcatttag acgcaatgaa gttcacttcg ccgattgaag aggcgaaagc ccaaattgcc
     2521 gatctcaaag ccaaaggcgt ggacgccatt attgtgctgg cacatatggg gattgagaac
     2581 gaaaacaaca ttcccgacac cggcgtggcg gatttagtga atgcagtgga cggtattgat
     2641 gtaattatcg ccggccatat gcataaagat gtgccgagtg aaactatcaa aaataccctg
     2701 attaccgaac cgcaccgcta cggcacagtg gtttccgaag ttgatcttgc ctttgatgtg
     2761 aatgacaaag gcgaagtgaa actactttct aaaaccgcca aaaccgtacc ggtgaagaaa
     2821 ctcgcaagcg atccggaaat cgaaaaaatt taccaaccgt atcacaatga actgcgtgcc
     2881 ttaaacaacg tcaaaatcgg tgaaactgca caagatttaa tcccacaagg caaaatcacg
     2941 gtgtatcgat tgctttctcg caagacaccg gaatgtcctc attgatccac gatgtgcaac
     3001 aacattacag cggtgcagac gtagtgagtt ttgcatttga ctacgaaacc gtgcgtttag
     3061 acaaaggcga catcaagaaa aaagacatca tctacaacta ccgctatgcc ggtggcgatg
     3121 tgagcatgta tgagctaacc ggcaaacagc ttaaacagta tatggaatgg tcagcggatt
     3181 acttcgacac cattcaagcc ggtgacaaag actaccgcta caacgctgtg cggggtaaat
     3241 ctaaatacgt taccttcgac ctattcggcg gcgtaagcta caacatcgac ctacgcaatc
     3301 caagcggtag caaaattgtc gatctaaaac tggcaaacgg cagtctaatc accgatgacg
     3361 gcaaatacaa agtgggaatg aactcctacc gctttggtca gctcaccaaa aaaggcggca
     3421 tctgggaagg acagtaaatc cccgtgattt gggaatccaa agtcgccatg ggggctgaaa
     3481 aaggcacaat ccaaaatatg ctgattgatt acatcaccaa cgtcaaaggc ggaaaaatcg
     3541 aaggcaaatc gcataaccgc tgggcgatta tcggcttaaa ttaacgaaag ggaaacacta
     3601 tgaacttaaa acaatctctt attgaaaata actctatttt gctcaatcaa tcagcggcga
     3661 attgggaaga cgcaatcaaa atcggcactg atttattagt caaagccggt acgattgaac
     3721 cacgctatta cgataatatt gtaagcaaaa tcaaagagat ggggccttat attattcttg
     3781 caccgggctt ggcaatgcct cacgcccgtc cggaagaagg tgtgattaaa acctctttcg
     3841 ctttagtgac ttttgacacc ccgatttatt ttgagggcga agatgagccg gttcacgtta
     3901 tgctaaccct tgcagggagt gactctgatc agcatatgca aggcttggta gaaatcaccc
     3961 aagtgctaga tgacccagag agcgacacag gtgttgattt agataaaatc cgccgctgta
     4021 aaacggctga agaagtgtat gcagtaatcg atcaggcatt agcctaatat tctacaagcg
     4081 gttcaaaaac atcaatattt tgcaaataga ggaatacaat tatgtccaaa ccattattac
     4141 aaattgccct tgattctctt tcactggaaa aagcggtggc ggatgcaaaa caagcagaaa
     4201 accaagtcga aattattgaa tgcggtacta ttcttgcttt tgctgagggt atgaaagccg
     4261 tttccacctt gcgtgtattg cacccaaatc acattattgt gtgcgactta aaaaccaccg
     4321 atggcggtgc aattttagct aaaatggcat ttgaagccgg tgccgactgg ctcacggttt
     4381 cggctgcggc tcaccctgca accaaagcgg cttgcaaaaa agtggcggac gaatttaacg
     4441 ctgctcaccc ggagctaaaa gtgaaaaaag agattcaaat tgaaatctac ggcaactgga
     4501 cggtggaaaa agatgccaaa gagtgggtag aattaggggt aacccaagcg atttaccacc
     4561 gctcccgtga tgcggaacta gcaggtaaag gttggacaac ggaagatgtt gagttaatga
     4621 aacagctttc tgacttaggc attgaattat ctatcaccgg cggtatcgta ccggaagata
     4681 ttcacctgtt caaagagatc aaaaacgcca aagcctttat tgcaggccgt gctttagtcg
     4741 gtgaaaaagg caaacaaact gccgaagcca ttcgtgtgga gattgataaa tactggaact
     4801 aatccggcat tgaaacccaa taaaaatggc gttctctcaa ccggaacgcc attttttatc
     4861 tcattttttc tattcttgca aggccgatat tttccacaat ctcgctacat tttcggcagt
     4921 tgaaatcagg ttctctttgc ctgattttaa caccgcttct aaattgctga cctgtcgaat
     4981 aatcggaaat accgcatcaa tgccgtgttg gtacaccaca gggtaatctt ctttcaatga
     5041 gccaactatc gcaatcaccg gcttgccaaa ctgcttagcg actttcgcca ccccaatcgg
     5101 ggttttaccg gcgatagatt gagcgtccat tcgcccttcc cctgtcacca ccaaatccgc
     5161 atcggcaagt ttctcagcaa gatcggttgc ctcaagaata atttgcacgc cggaacgtaa
     5221 tgttacgtta ggcagtagca gtaaaccacc gcccattcca ccggctgcac cggcaccggc
     5281 tttctcggca atctcaatgc ctaattggga ttttacggtt tcggcaaaat gcaacagggc
     5341 gtggtcgagc attttaacca tttccggtgt agctcctttc tgtggcccaa aaattgccga
     5401 agcccctcgc tcgccacaca gcggattatc gacatcgcaa gcaacctcaa attccacttg
     5461 ctttaagcgt gaatccaaac cgcttaaatc aatctgctga atttggctta atgcctcgcc
     5521 accaaagccg atttcccgct gttcggcatt tagaaatttc gctcctaagg cttgtagcat
     5581 tccgacaccg ccatcgttag tcgcactgcc gccaatgccg agaatgattt tttccacccc
     5641 taaatctaag gcggctttaa ttaactgccc cgtgccgtag ctggtggtaa ccagcggatt
     5701 tcgttgctcc attggcacta aatgcaagcc acttgccgcc gccatttcaa taaaggcggt
     5761 tttttgatcc cccgacaagc caaaaaagcc ctccacctca tttgcaagcg gtgcaattac
     5821 gctgattttt tgcaaactgc cgtgagtggc atcaaccagc gactgtaccg agccttcgcc
     5881 accgtctgcc atcggtacga gctgatactc cgcctcaggg aaaacacggg caaaaccctg
     5941 ttgaattgct tgagccactt ctaatgcggt caaactctct ttaaatgaat ccggtgcaat
     6001 cacaattttc atcgtcaatc cttagagtaa aatcaatgaa agcagataaa ccgtgagcat
     6061 acccaccaca cccataatga aagttagggt agtttgcgta cgatagcctt gttgcggagt
     6121 cagtttagta aagtttgtta ccacccagta gtagctgtca ttggcgtggg aaacgcacat
     6181 tgagccagcc gcaatcgcca ttacggttaa ggctgccgcc atttcactgg ttagccctaa
     6241 ggcgttcatt aatgagtccg gtgcgttgaa cattcccata atggaggcgg tggtgataat
     6301 tgccaccgtt gagctgcctt gtgcggtttt taaaattgcc gaaatcagga acgggaagaa
     6361 aatccctgct gtactgataa tatgagcgtt ttgcttgatg taatccacaa agccggcttg
     6421 agtaatcacg ttacctagca caccaccggc ggcggtaata aagagaattg ggccgactaa
     6481 tttcagggta tcattggtta agctgtcaaa ttccgccatt ttaccgcttg aaaccagtag
     6541 gaaaatagcg aagatcaccc caattgctaa cgcaataatc gggttgccga caaattgtag
     6601 gaatacgccg aaatcgccac ttacaccggc aattttggca atcgagccga tagccattaa
     6661 gataatcggc ataaaaatcg gggcaaagct taaaaaccca cttggtaatt tgccgtattg
     6721 tttgatcaga tcttcatagc tttggctgat tacttgatcc gcttgagctt cttccatcac
     6781 gctgacttgt ttgccaacat atttggcaaa gaagtaactg gcaatcaaca caggaatcga
     6841 aaccacaaag cccattccga tcacaagcag gagattatgg cttaaaccaa cagcaccggc
     6901 tgctgcaatc gggcccggtg tcggaggaat aaagacgtgg gaggcatata aaccggcact
     6961 taacgccacc gccattccca ccggattggc aagaattttt ttgcgaatag cttcacgaat
     7021 cgggtttaac accacaaagc cgctatcaca gaacactggg ataccaacca cccagcccat
     7081 aatcaacatt gccagttcag ggcgtttttg ccccaccact tttaccacca tatcggctaa
     7141 tttgagggct gcaccggttt tttctaaaat cgtgccgata atcgccccaa aaataatcac
     7201 gataccgata cttttgaagg tattactaaa accttcgccc acaatagttg gaattttggc
     7261 aagcggtaaa cctgccacta atgcaagggc tatcgaaatc gacattaagg ctaagaacgg
     7321 gtgaactttc cacttcgcaa tcatataaat cattatgatg atagcgacca gaaagatcag
     7381 aataagtgca atacctgaca taataagtct cctcattgta gaaa
//
LOCUS       DANLUQ010000125         7391 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.126, whole genome shotgun sequence.
ACCESSION   DANLUQ010000125 DANLUQ010000000
VERSION     DANLUQ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7391
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.126"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..507
                     /locus_tag="PXB27_001987"
     CDS             <1..507
                     /locus_tag="PXB27_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein"
                     /protein_id="HDL5346745.1"
                     /translation="LFIAQIKQNLSELNVFSTDNIFLNSPYFSQQTGLVSVFIAEIEK
                     TVELLLNQTEVLYSEFYAEKLVKQVDALKNAVEKIQSKPESAQFHSSYQFSPNIHRLA
                     PNKRLQEYRKALRALNEKISWLVEQNLNTQNEATKQTLQNQITETEYRKMKCLKAIED
                     LEQELLFK"
     gene            complement(511..584)
                     /locus_tag="PXB27_001988"
     tRNA            complement(511..584)
                     /locus_tag="PXB27_001988"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(550..552),aa:Cys,seq:gca)
     gene            complement(600..675)
                     /locus_tag="PXB27_001989"
     tRNA            complement(600..675)
                     /locus_tag="PXB27_001989"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(640..642),aa:Gly,seq:gcc)
     gene            complement(885..2309)
                     /gene="lpdA"
                     /locus_tag="PXB27_001990"
     CDS             complement(885..2309)
                     /gene="lpdA"
                     /locus_tag="PXB27_001990"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011272714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDL5346746.1"
                     /translation="MSKEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYSTLGGI
                     CLNVGCIPSKALLHVAKVIEEAKNAVHNGVTFGEPTIDLDQVRAGKEAVVSKLTGGLA
                     GMAKARKVTVVEGLAAFTGPNTLVARDRDGNPTTITFDNAIIAAGSRPIQLPFIPHED
                     PRVWDSTDALKLKEVPKKLLIMGGGIIGLEMGTVYNALGSEVEVVEMFDQVIPAADKD
                     VVAIYTKQIEKKFKLMLETKVTAVEAKDDGIYVSMEGKACNDTKRYDAVLVAIGRVPN
                     GKLIDAGKAGVEVDERGFIRVDKQMRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGMKHYFDPKVIPSIAYTEPEVAWVGKTEKECKQEGLNYEVAKFPWAASGRAIASEC
                     SEGMTKLIFDKDTHRVLGGAIVGSNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNAKAKKR"
     gene            complement(2509..4419)
                     /gene="aceF"
                     /locus_tag="PXB27_001991"
     CDS             complement(2509..4419)
                     /gene="aceF"
                     /locus_tag="PXB27_001991"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HDL5346747.1"
                     /translation="MSKQINVPDIGSDEVSVTEVMVKVGDTISVDQSIINVEGDKASM
                     EVPAPEAGVVKEVLVKVGDKVSTGSPMLILESADAAPAQEAPKAEAAPAAPVATTSAV
                     VEVNVPDIGGDEVNVTEIMVKVGDSVEVDQSIINVEGDKASMEVPAPVAGVVKEILIN
                     VGDKVSTGKLIMKFETASAATAQSTPVAEAAPAQASASAIKDVNVPDIGGDEVNVTEI
                     MVKVGDSVSEEQSLITVEGDKASMEVPAPFAGVVKEILVKSGDKVSTGSLIMKFEVAG
                     AAPAPAAAQAASAPAPAPQAAAPAKADAAPAGQNQSGLSQEQVVASAGYAHATPVIRR
                     LAREFGVNLDKVKGTGRKGRIVKEDIQAYVKTAVQVFEKQGGTAAAATGAANGAGLGL
                     LPWPKVDFSKFGEVEEVELSRINKISGANLHRNWVMIPHVTHFDRTDITELENFRKEQ
                     NKEAEKRKLDVKITPVVFIMKAVASALEAFPRFNSSISEDAQRLTLKKYINIGVAVDT
                     PNGLVVPVFKNVNKKGIIELSRELMEVSKKAREGKLTASDMQGGCFTISSLGGIGTTH
                     FTPIVNAPEVAILGVSKSEMQPIWNGKEFEPRLMLPLSLSFDHRVIDGADGARFLSYI
                     NSVLADIRRLVM"
     gene            complement(4623..7280)
                     /gene="aceE"
                     /locus_tag="PXB27_001992"
     CDS             complement(4623..7280)
                     /gene="aceE"
                     /locus_tag="PXB27_001992"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005628834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HDL5346748.1"
                     /translation="MSENLRNDVDPIETQDWLASLDSLIREEGLERAQFIIEQVISQA
                     RAGGVALPTGVTTDYVNTIPVSEQPAYPGDHKIERRIRSAVRWNAIAAVLRSQKKDLD
                     LGGHISTFQSAATMYEVCFNHFFKAPTEKDGGDLVFFQGHAAPGMYGRAFLEGRITAE
                     QMDNFRQEAFTDGLSSYPHPKLMPEFWQFSTVSMGLGPVNAIYQARFLKYLENRGLKD
                     TSAQKVYAFLGDGEMDEIESKGALSFAGREKLNNLIFTISCNLQRLDGPVNGNGKIVQ
                     ELEALFTGCGWEVIKVLWGSGWDKLLEKDTSGKLKQLMMEVLDGDYLTFKSKNGAYVR
                     EHFFGRYPETAALVADMTDDEIWALRRGAHDSEKLYAAYAKAQKSDKPVVILAHQVKG
                     YRIPEAESKNTAHQSKKMSLDSLKGFRDYFELPLTDEQVESLEYVRFPEGSEEYNYLH
                     GQRKALNGYVPVRKPKFTVDFKVPELSEFQALLDEQPRGISTTMAFSRVLNTLLKDKN
                     IGQQIVPIIADEARTFGMEGLFRQIGIYNPHGQNYVPSDRDLVAYYREAKDGQVLQEG
                     INELGATASWVAAATSYSVSNLPMIPFFIYYSMFGFQRVGDMMWLAGDQLARGFMIGG
                     TSGRTTLNGEGLQHEDGHSHIQAGVIPNCVTYDPAFAFEVAVIVQDGIRRMYGENQED
                     IFYYITTLNEITEQPAMPAGAEEGIRKGLYKFETVEGKGKGHVQLLSSGAIMRHVRAA
                     AQILANDYGITADVFSAPSFNELGRDGADVARWNLLHPTETPRVPYVAQVLQDLPTVA
                     STDYMKMYAEQIRAYVPSKHYHVLGTDGFGRSDSRANLREHFEVDERYVVVAALAQLA
                     KEGTVENKVVAEAITKFGLNVDRINPLYA"
BASE COUNT         2093 a         1674 c         1388 g         2236 t
ORIGIN      
        1 ctttttatcg cccaaataaa acaaaacctc tcggaattaa atgtattttc aacggataat
       61 atttttctga attcacccta tttctcacag caaaccggtt tggtgagtgt gttcattgct
      121 gaaattgaaa aaacagttga gctattattg aaccaaactg aggtgttata tagtgaattt
      181 tacgctgaaa aattggttaa acaagttgat gcattaaaga atgccgtaga aaaaatccaa
      241 agtaagccag aatcagctca attccattct tcctatcaat tttctccaaa tattcatcgt
      301 ttagcgccca acaaacgctt acaagaatac cgtaaagcgt tacgtgcatt gaacgagaaa
      361 attagctggt tagttgagca aaatttaaat acgcaaaatg aagcaactaa acaaaccctg
      421 caaaatcaaa ttacagagac agaatatcgc aaaatgaaat gtttaaaagc aattgaagat
      481 ttagaacaag agttgctatt taaatagtga tggaggcgtg tcccggagtc gaaccgagct
      541 acatggattt gcaatccagt gcataaccgc tttgctaaca cgccataaga tttggaaatt
      601 ggagcgggaa acgaggctcg aactcgcgac cccgaccttg gcaaggtcgt gctctaccaa
      661 ctgagctatt cccgcatctg aattcccaca acattgcgct gtggaacgag gtgcatttta
      721 cggatttatc attttatgtc aatgctaaat ttaacattct agattcaaat gctgaaaaaa
      781 aactcactat tcaatagttt agttttaaaa ataatcaagc ggttgtattt tgcaaaaaat
      841 ttacaaaata caaccgctta ttttttagag attaaataat ctgattaacg ctttttggct
      901 ttcgcatttg gtaagtctgt aattgaacct tcaaatactt ctgccgctaa tcctaccgat
      961 tcgtgtaacg ttgggtgagc atgaatggtt aatgcgatat cttcagcatc acagcccatt
     1021 tcgattgcaa gaccgatttc gcctaataat tcgccaccat tgctaccaac aatcgcacca
     1081 cctaatacac ggtgagtatc tttatcaaaa attaacttgg tcataccttc tgagcattca
     1141 gaagcaatcg cacgacctga agcagcccaa gggaatttcg ccacttcgta gttcaagcct
     1201 tcttgtttac actctttctc agttttacca acccaagcca cttctggttc agtataagcg
     1261 atggacggaa tgactttcgg atcgaagtaa tgtttcattc ctgcaatcac ttctgctgcc
     1321 acgtgtccct cgtgtacacc tttgtgagct aacatcggct ggccgacaat atcaccaata
     1381 gcaaagatgt gtggcacatt ggtacgcatt tgtttatcta cacgaataaa gccacgctca
     1441 tctacttcca ctccagcttt accggcatca attaatttac cgtttggcac acgtccgata
     1501 gcgactaata ccgcatcata acgtttagta tcgttgcaag ccttaccttc cattgataca
     1561 tagataccgt catctttagc ttctactgcg gttactttgg tttcaagcat tagcttgaat
     1621 tttttctcga tttgtttggt gtaaatcgct acaacatctt tatctgcagc aggaattact
     1681 tggtcgaaca tttcaaccac ttccacttct gaaccaagcg cgttatatac cgttcccatt
     1741 tctaaaccga taataccacc acccataata aggagttttt tcggtacttc ttttaattta
     1801 agagcatcgg ttgaatccca aactcgtggg tcttcgtgtg gaataaatgg tagttgaatc
     1861 ggacgagaac ctgctgcgat aatcgcatta tcgaacgtga tagttgtagg attgccgtca
     1921 cgatcacgag cgactaacgt attcggacca gtaaatgcag ccaagccttc tactactgtc
     1981 actttacgag ccttagccat tcctgctaag ccaccggtta attttgaaac aacggcttct
     2041 ttaccagcac gtacctgatc taaatcaatc gttggctcac caaaggtcac accgttgtgt
     2101 actgcatttt tagcttcttc aattactttt gcaacgtgta ataatgcttt agaagggata
     2161 caacctacgt ttaagcaaat accacctaag gttgaataac gctcaacaat tactgtttct
     2221 aagcctaagt cggcacaacg gaatgccgct gagtaacctg ctggacctgc accaagtacg
     2281 acaacttggg ttttaatttc tttgctcata gtttttcctc tttttattcc ccccctttag
     2341 caaagagggg taagggagat ttggcagaat ataaagcgaa gtgagagagc aatcaatcaa
     2401 acacaaattt ctaccaaata atttattctc ttcgtaaatt acgtctatca aatctccccc
     2461 tacccctctt ttctaaagag ggactttttt aggaggagct ttaccgaatt acatcactaa
     2521 acgacgaata tctgccaaca cgctattaat atagcttaag aatctcgcac catcagcacc
     2581 atcgatcaca cggtggtcga atgataatga aagcggaagc attaagcgtg gttcaaactc
     2641 tttgccattc caaatcggtt gcatttcaga tttagacaca cctaaaatag ccacttccgg
     2701 tgcattcacg attggcgtaa agtgcgttgt accaataccg cctaaacttg aaattgtgaa
     2761 acagccacct tgcatatcag atgctgtcaa tttaccttca cgggcttttt tcgagacttc
     2821 cattaattcg cgagaaagtt cgataatgcc ttttttgttc acatttttga acaccggcac
     2881 cactaaaccg tttggcgtat caaccgcaac cccgatattg atgtattttt tcaaggttaa
     2941 acgttgtgca tcttcagaaa ttgagctgtt gaaacgaggg aaggcttcta atgcacttgc
     3001 cactgctttc ataatgaaga ccacaggggt aattttcacg tctaatttac gtttttccgc
     3061 ttctttattt tgctctttac ggaagttttc taactcggta atgtctgtgc gatcgaaatg
     3121 tgtaacgtga ggaatcatta cccagttacg gtgtaagttc gcaccggaaa tcttattgat
     3181 acggcttaat tcaacttctt caacttcacc aaacttgctg aaatctactt tcggccacgg
     3241 taataaacct aagcctgcac cgtttgccgc acctgttgct gccgctgctg ttccgccttg
     3301 tttttcaaat acttgaacag ccgttttaac gtatgcttga atatcttctt taacgatacg
     3361 gcctttacga cctgtacctt ttactttgtc taggtttaca ccaaactcac gagctaaacg
     3421 gcgaattacc ggtgttgcgt gtgcatagcc agctgaagcc accacttgtt cttggcttaa
     3481 acctgattgg ttttgacctg ccggagctgc gtcagctttc gccggagctg ctgcttgtgg
     3541 agcaggagca ggagctgaag ccgcttgagc agccgccgga gccggtgccg cacccgctac
     3601 ttcaaatttc ataattaatg aaccggttga aactttatca cctgatttca ctaaaatttc
     3661 tttcaccaca ccggcaaatg gagccggaac ttccattgaa gctttatcac cttcaaccgt
     3721 gattaaagat tgttcttctg aaacagaatc acccacttta accataattt cagttacgtt
     3781 gacttcatca ccaccgatgt ccggtacatt tacatcttta atcgctgatg ccgaagcttg
     3841 agctggagca gcttccgcaa ccggtgtaga ttgtgctgta gctgcggaag ccgtttcaaa
     3901 tttcataatc aatttaccag ttgaaacttt gtcacccaca ttgattaaga tttcttttac
     3961 tacgccggca accggtgcag gcacttccat tgaggcttta tcaccttcaa cgttgatgat
     4021 agattgatcc acttcaacgc tatcacctac tttaaccata atttcggtta cgttaacttc
     4081 atcgccaccg atatcaggta catttacttc aacaacggct gaagttgttg ctactggagc
     4141 agcaggagct gcttctgctt ttggtgcttc ttgagcagga gcagcatctg ctgactctaa
     4201 aattaacatt ggtgaaccgg ttgaaacttt atcacccact ttcactaaaa cttcttttac
     4261 cacacctgct tcaggagccg gcacttccat agaggcttta tcgccttcta cgttaataat
     4321 tgattgatct actgaaatag tatcaccaac ttttaccatc acttcagtca cactgacttc
     4381 atccgaaccg atgtccggca cattaatttg ttttgacatt ttgaaatcct tcttgagtta
     4441 ccccctctcc cattgtggga gggacagatt tttctttgta agaagaaaaa tcagggagaa
     4501 gggaatgcaa gcggttgatt ttctcaacat ttttgcaaat aaaactaata ccctctctgc
     4561 taaaagtttc taaacgaaac ctttagctgc ctctctccca tcaagggaga gagtattaga
     4621 aattacgcat ataatgggtt aatacgatct acatttaagc cgaatttagt aatagcttcc
     4681 gcaaccactt tgttttcaac tgtaccttct ttcgctaatt gagcgagggc tgcaactact
     4741 acataacgct catcaacttc gaagtgttca cgtaagtttg cacggctgtc tgaacgaccg
     4801 aaaccgtctg tacctaacac gtgatagtgt ttacttggta cataagcacg gatttgctct
     4861 gcgtacattt tcatatagtc ggttgaagca acagtcggta aatcttgtaa cacttgagca
     4921 acgtacggta cacgtggtgt ttcagttggg tgtaataagt tccaacgagc cacatctgct
     4981 ccatcacgac ctaattcatt gaatgatggt gctgagaata catctgctgt aatgccgtag
     5041 tcattcgcga gaatttgtgc agctgcacgc acgtgacgca taattgcacc tgagcttaat
     5101 aattgtacgt gacctttacc tttaccctca acggtttcaa atttatataa acctttacgg
     5161 ataccttctt ccgcacctgc cggcattgcc ggctgttcgg taatttcatt taaggtagta
     5221 atgtagtaga agatatcttc ttggttttca ccatacatac gacgaatacc gtcttgaaca
     5281 ataaccgcaa cctcaaaggc aaatgcaggg tcataagtga cgcagttcgg gattacacct
     5341 gcttgaatat ggctatgacc atcttcgtgt tgtaaacctt caccgtttaa tgttgtacga
     5401 cctgaagtac caccaatcat aaagccacga gctaattggt caccggctaa ccacatcata
     5461 tcgcctacac gttggaaacc aaacattgag tagtagatga agaatgggat catcggtaag
     5521 ttgctaacag aataagaagt cgctgctgcc acccaagagg ctgttgcacc taactcattg
     5581 ataccttctt gtaacacttg gccatctttt gcttcacggt agtaagctac taaatcacgg
     5641 tctgacggta catagttttg accatgtggg ttataaatac cgatttgacg gaataaacct
     5701 tccataccga aagtacgggc ttcgtccgca ataataggta cgatttgctg accgatattt
     5761 ttatctttca ataaagtatt caatacacga gagaacgcca ttgtggttga aataccacgt
     5821 ggttgctcat ccaataatgc ttggaattcg cttaattcag gcactttgaa atcaaccgta
     5881 aatttcggtt tacgtacagg cacataaccg tttaaggctt tacgttgtcc gtgtaagtag
     5941 ttgtactctt cagaaccttc agggaatctt acatactcta ggctttctac ttgctcatcg
     6001 gttaatggta gctcgaagta gtcacggaag ccttttaagc tgtctaatga catttttttc
     6061 gattggtgag cagtattttt actttccgct tcaggaatac ggtagccttt cacttggtga
     6121 gctaaaatta caaccggttt gtccgatttt tgtgcttttg cataggctgc ataaagtttt
     6181 tcagaatcgt gtgcaccacg gcgtaatgcc caaatttcat cgtctgtcat atcagcaact
     6241 aatgctgcag tttcagggta acgaccgaag aagtgttcac gaacgtaagc accatttttg
     6301 gatttgaagg ttaaatagtc accatcaagc acttccatca ttaactgttt aagtttgcct
     6361 gaagtatctt tttcaagcag tttatcccaa ccactacccc ataacacttt aatcacttcc
     6421 cagccacaac cggtaaagag tgcttctaat tcttgcacga ttttgccgtt accgttaacc
     6481 ggaccgtcta aacgttgtaa gttgcagcta atagtgaaga ttaagttatt taatttttca
     6541 cgaccggcga atgataatgc acctttagat tcgatttcat ccatttcgcc atcgcctaag
     6601 aatgcatata ctttttgagc cgaagtatct tttagaccac ggttttctaa atattttaag
     6661 aaacgagctt ggtaaatagc atttaccggt cctaaaccca tagatacggt agagaattgc
     6721 caaaattcag gcattaattt agggtgtggg taagaagata aaccgtctgt aaatgcttct
     6781 tgacggaagt tatccatttg ttctgcggta atacgacctt ctaagaacgc acgaccatac
     6841 atacccggtg ctgcgtggcc ttggaagaac actaaatcac caccatcttt ttcggttggt
     6901 gctttgaaaa agtggttaaa acacacttca tacatagttg ccgcagattg gaaagttgaa
     6961 atatgaccac ctaagtcaag atctttcttc tggctacgaa gtactgccgc aatcgcattc
     7021 caacgtaccg cagaacgaat acgacgttcg attttatgat cacccggata agctggctgc
     7081 tcggaaacag ggatagtatt gacataatct gttgttacgc ctgttggtaa tgccacgcca
     7141 cctgcacgag cttggctaat aacttgttca atgataaatt gagcgcgctc taaaccttct
     7201 tcacgaatta atgaatcgag tgatgctaac caatcctgag tttcgattgg atctacgtca
     7261 tttcttaagt tctctgacat agtattcttc cttataaatt aacgattgag gtgataaata
     7321 acaaaatttt aacaaatttt gtttgtttgg tgatttttag accaattctt atcgaaaaaa
     7381 tttcagtaaa a
//
LOCUS       DANLUQ010000126         7387 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.127, whole genome shotgun sequence.
ACCESSION   DANLUQ010000126 DANLUQ010000000
VERSION     DANLUQ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7387
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.127"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..620)
                     /locus_tag="PXB27_001993"
     CDS             complement(<1..620)
                     /locus_tag="PXB27_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HDL5346749.1"
                     /translation="MTELTPKQEAFCFAYIETGNASEAYRQAYETEDMKSETVHRKAK
                     ELMDNGKITARIEELKAEHAERHKLTVDDLLKELEEARILAREKENPNAMTQATMGKA
                     KLLGLDKQVTDPCFDGETGKGDMILSVNCSSIKDGKALDETCILKAGTHEFITRDSFI
                     FYRHIRIDSLDEIKANIEAGYFIQKALINDEAYQQILKGVLSSKSVT"
     gene            complement(617..1042)
                     /locus_tag="PXB27_001994"
     CDS             complement(617..1042)
                     /locus_tag="PXB27_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346750.1"
                     /translation="MKHELNLAGITALMNEPIKATLSFLDFPHIRIERLHHDNGRPFF
                     IGVLSLDLEDHINIEMAGATGFLEALAIIAYHQQLTATPLLVNDDLLSEINKLPHPQR
                     TNPFIDRHGNEITREQMSKEYREKIDYAFAEKRMAEGKE"
     gene            complement(1362..2048)
                     /locus_tag="PXB27_001995"
     CDS             complement(1362..2048)
                     /locus_tag="PXB27_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346751.1"
                     /translation="MADHSQFISRLENRIEPKISFQELIQELSNYTNKPFSHIADKLK
                     SLIFSFQQNGIYTNAFVNCTLFEDGESYAIEPKTLHFKDAFDQPKGVLSDVIAQNSID
                     VEHLNAYYVTAREITQLIKTQSPTLLDKLTTKQTQSEISQIQNDYISVYDFIEWASKH
                     YSSLSETANDLNRLLKDKNIQLYRQYGGISPSIDKDNTNLKAAFDFVAINNGYEKQSS
                     SFNFDDDIPF"
     gene            complement(2443..2766)
                     /locus_tag="PXB27_001996"
     CDS             complement(2443..2766)
                     /locus_tag="PXB27_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative addiction module antidote protein"
                     /protein_id="HDL5346752.1"
                     /translation="METIQINEQAAEKHGLRPFDVAEFLTDEATIQAYLAEVLKEGDQ
                     DEFLEALNDVARARGMAELAKQTGLGRESLYKTLAKGSKPRFDTIQKILGAFNLVLIP
                     QVKNG"
     gene            complement(2879..3097)
                     /locus_tag="PXB27_001997"
     CDS             complement(2879..3097)
                     /locus_tag="PXB27_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module killer protein"
                     /protein_id="HDL5346753.1"
                     /translation="MMREQTTNQIIITDNFSKWLENLKNPIAKATVARRIKNAINGNF
                     GDHKSLAGTGGLYEMRIATGAGYRIYYA"
     gene            complement(3372..4598)
                     /locus_tag="PXB27_001998"
     CDS             complement(3372..4598)
                     /locus_tag="PXB27_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL5346754.1"
                     /translation="MARKITPLNDTQIRKAKPEDSPLRDGNGLLLVITSNSKLWRFRY
                     ERPFTKKRNDLSIGAYPDISLAQAREIREEYRALLAQNIDPQTHRQQQEQAKANEISN
                     TYENVAWAWLEHRKTKKNFSDNYQKDVIALIKNNLLPHFGRMPITQITAPMAIKAFKQ
                     YQDEGKLEKLKRAIQKHNEIMMFAVHREMIPFNPTANIAKEFDSPTVEHFKTIKPEDL
                     GEFLYTLQNAQIHLQTRYLILWQLLTMTRPNEAAKAKWADIDEKAKLWTIPAENMKRG
                     IEHKITLSRQAIALLREIKKLSGGKTYLFPSVKNPQTHVNTQTANGAIKRMGYKGKLV
                     AHGLRAIASTYLNEKGYNSDLIEVALSHLNSDRIKAAYDRGERLEQRFKLLQVWADFV
                     EECSQGSLPQYHLKIA"
     gene            complement(4873..5239)
                     /gene="ssrA"
                     /locus_tag="PXB27_001999"
     tmRNA           complement(4873..5239)
                     /gene="ssrA"
                     /locus_tag="PXB27_001999"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            5405..6364
                     /gene="rpoH"
                     /locus_tag="PXB27_002000"
     CDS             5405..6364
                     /gene="rpoH"
                     /locus_tag="PXB27_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HDL5346755.1"
                     /translation="MKKFNENEIEDAEIIDAEPIDVDEIDEEETSVEAMHPALVSGSM
                     PVPQGNLDSYIRMANQYPILTAEQEKELAERYYYDEDVEAAKQLILSHMRFVIHIARG
                     YLGYGLPLADLIQEGNIGLMKAVKRFDPNVGVRLVSFAVHWVKAEIHEYVLKNWRIVK
                     VATTKAQRKLFFNLRKNKNRLAWFNEEEIKKVADDLGVSVEEVREMESRMTGQDLGFD
                     LPAGDDDEDSYAPSMYIEDSSSNFADDLEDEQHNGQATAQLAYALATLDERSQDIIKT
                     RWLDDNKATLQDLADKYNISAERVRQLENAALKKIKEAISLDG"
     gene            6530..6823
                     /locus_tag="PXB27_002001"
     CDS             6530..6823
                     /locus_tag="PXB27_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3144 domain-containing protein"
                     /protein_id="HDL5346756.1"
                     /translation="MTQLSTSVYQRADGFINVANAHLKNIAPNQVSNAMLFGCARFNA
                     YVAASKAEYKQQLADSREEVIQYFVEQYKEMLTANLDEYIQNFERYIEGKKAD"
     gene            complement(6841..7143)
                     /locus_tag="PXB27_002002"
     CDS             complement(6841..7143)
                     /locus_tag="PXB27_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HDL5346757.1"
                     /translation="MNTYNNTLNNKNLEKKQLLMAIQAIPASQDFIWDGKDDDERPVT
                     PSEFSIAMKKRGRPAGSKKELVSLRLDKSVLEAFRASGKGWQSRINQVLSDFVKQV"
     gene            complement(7115..>7387)
                     /locus_tag="PXB27_002003"
     CDS             complement(7115..>7387)
                     /locus_tag="PXB27_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HDL5346758.1"
                     /translation="DETKRKINLAKHGLDFADADEVLSSPYRFDVEVIRNNEFRIQSF
                     AYSFGRLLVLSLAYTEREDYIRVISFRRASKAEREAYYEYLQQYFE"
BASE COUNT         1986 a         1449 c         1528 g         2424 t
ORIGIN      
        1 gttactgatt ttgacgatag tacgcctttt agtatttgtt gataggcttc atcattaatt
       61 agggcttttt gaatgaaata accggcttct atatttgcct taatctcatc taacgaatcg
      121 attcttatat ggcggtagaa aataaagctg tcccgtgtga taaattcgtg tgttcctgcc
      181 tttaaaatac aagtttcatc aagtgctttc ccgtctttaa tgcttgagca gtttacactt
      241 aaaatcatat ctcctttacc ggtttcacca tcaaagcacg gatcggtaac ctgcttatcc
      301 agccctagca acttcgcttt gcccattgtg gcttgagtca ttgcgttagg gttttctttc
      361 tctctcgcca gtatacgagc ctcttccagc tcctttaaaa ggtcatcaac ggttaattta
      421 tggcgttctg cgtgttcggc tttcagttcc tcaatccttg ccgtaatctt gccgttatcc
      481 attagctctt tagcttttcg gtggacggtt tcggacttca tatcttcggt ttcataggct
      541 tggcggtagg cttcgctagc attgccggtt tcaatataag caaagcaaaa agcctcttgt
      601 tttggtgtga gttcggtcat tctttcccct ccgccattcg cttttcagca aaggcataat
      661 cgattttttc tcggtattct ttgctcatct gctcccgtgt aatttcgttg ccgtgtcggt
      721 caataaatgg gtttgttcgt tgtgggtggg ggagcttgtt gatttcactc aataagtcat
      781 cattcactaa tagcggcgtg gcggttagct gttgatgata ggcaataatc gctagagcct
      841 ctaaaaagcc cgtagcccct gccatttcaa tattgatatg atcttcaagg tcaagagaaa
      901 gcacaccgat aaaaaacggt ctgccgttat cgtggtgcag tcgttcaatt cggatatgcg
      961 gaaaatctaa aaagctcaag gttgctttga taggttcatt cattaaagca gtaatacccg
     1021 ctaaattaag ctcgtgtttc attttggttt attcctttat tctttccgcc acttttaaca
     1081 gttggctaag ttgttttctt aaaatgccga gtgtgattga atcggtttcg cttggcattg
     1141 cgttatgttg ttgttccagc ttcgctattc ggttgcctag cacaattaaa agctcgggca
     1201 atgggtaagc tcggtttcag aatagaggct taaaaacgtg aatagtctgc taaataatca
     1261 ggtgttatct cagaaaaatc gagctttttc cctagtgctg cttctaattc ttgtttattt
     1321 aaaagtaaat tttggaaatc atttaaattc attgctgcca cctagaacgg aatatcatca
     1381 tcaaaattaa agctgcttga ttgtttttca tatccgttat taattgctac aaaatcaaag
     1441 gctgctttta aattagtatt atctttatca atgcttggac ttattccgcc atattgtcga
     1501 taaagctgta tatttttatc tttgagtaag cggtttaaat cgttagctgt ttcactaaga
     1561 cttgagtaat gtttacttgc ccattctatg aagtcataaa cagaaatata atcattttga
     1621 atttggctaa tctctgattg tgtttgcttg gtggttaatt tatctaacaa ggttggcgat
     1681 tgtgttttaa tcagctgtgt tatttctctt gctgttacgt aataagcatt taaatgttca
     1741 acatcaatag aattttgagc aataacatca cttaaaacgc ctttaggctg atcgaaagcg
     1801 tctttgaaat gtagtgtttt gggctcaata gcatagcttt caccatcttc aaagagagtg
     1861 caatttacaa aagcattcgt atatattcca ttttgctgaa aggaaaaaat gaggcttttc
     1921 aatttatccg ctatatgtga aaaaggttta tttgtatagt ttgataattc ttgtatcagt
     1981 tcttgaaagc tgattttggg ctcaatacga ttttcaagcc tagaaataaa ttgactgtga
     2041 tctgccataa gcgccccttt aacgtatccc tttactgtga gagcgtggaa agagaaataa
     2101 agggctttct cctttcgtgc cggttaatta ctccgccact atccacgcta atttgaataa
     2161 atccgtatag ttacggattg aacttgatag gttgtatttt gctaattatt cagataaatt
     2221 acaagtaata agctgtgttt tattacagta aatcaaaata ggctaaagat gggtttccgg
     2281 acgctataaa aaagagcatt caagcggtca tatttcatta aggttttgcg aatagtgcgg
     2341 aattttcgga gcagtacgca cgcaaggagc aaataaaaag ccgtcaatat ccatcaacgg
     2401 ctatttaaaa gtgaggtttt gacaggtagc aagtgataag ttttagccat ttttgacttg
     2461 tggaataagc actaaattga atgcccctag aattttttga atagtgtcaa agcgtggttt
     2521 gctgcctttg gctagggttt tatataggct ctctctgcct agccctgttt gcttggccag
     2581 ctccgccatt cctcttgctc ttgctacatc gttcaaggct tctaaaaatt cgtcttggtc
     2641 gccctctttt aacacttccg ctaaataggc ttgaatagtt gcctcatcgg ttaaaaactc
     2701 cgccacgtca aaagggcgta aaccgtgctt ttctgctgcc tgttcattga tttgtattgt
     2761 ttccattgct ttctcctgta gtttttaatt cagcccataa tgctttcgct ttctctatat
     2821 cggcttgctg tgttgattta tcgccgccgt gtaacaagat ataaattaca tttccttgtt
     2881 atgcgtaata aattctatat ccggctcctg ttgctatccg catttcataa agtccgcctg
     2941 taccggctaa agatttatga tcgccaaagt tgccattaat cgcatttttt atccgtcttg
     3001 ctaccgttgc ttttgcgata gggttcttta aattctcaag ccattttgaa aaattatcgg
     3061 taataataat ttgattagtg gtttgctctc tcatcattgc cggcttctta ttctatttat
     3121 tgcttattgt atccattaag atacagtgag caagggtttt tgccatttac ccacttttaa
     3181 cccactttac ccactgtcat tttttcttta tttttcagtt agttataaaa aaataatagt
     3241 gaaaagtggg taaagtgggt aaaaatcgaa aagtttacag aattttcatc aaggggaatt
     3301 acgggcataa aaaagccgtc aggcggttta cctaacggca ttacaagcgg tcatttttgg
     3361 cggaaaattt actaggctat tttcaaatga tattgcggta agctaccttg cgaacattcc
     3421 tctacaaaat ccgcccacac ttggagcagt ttaaaacgtt gctctaggcg ttcgcctcta
     3481 tcataggcgg ctttgattcg gtcgctgttt aaatgcgata aggcaacctc gattaaatcg
     3541 ctattgtagc ctttctcatt aagataagtg gaggcgatgg cccgcaagcc gtgagccact
     3601 aatttgccct tgtagcccat tcgtttgatc gcaccgttgg cggtttgagt attgacgtgc
     3661 gtttgtgggt tcttcacgct agggaataaa tacgttttac cgccacttag ctttttaatc
     3721 tctcgcaaaa gtgcgattgc ttggcgtgag agtgtgattt tatgctcaat gcctcgcttc
     3781 atattttccg ccggtatcgt ccacagtttg gctttttcgt ctatatccgc ccattttgcc
     3841 ttagccgctt cattcgggcg ggtcattgtg agtaactgcc acagaattaa atagcgtgtc
     3901 tgtaaatgaa tttgggcgtt ttgtaaggtg tataaaaact cgcctaaatc ttccggctta
     3961 atggttttga aatgttcaac cgttgggcta tcaaactctt tcgcaatatt ggcggtaggg
     4021 ttaaacggta tcatttcacg gtgtacggca aacatcataa tttcattatg tttttgaatt
     4081 gcccgtttta acttctctag tttgccctcg tcttggtatt gcttgaatgc cttgattgcc
     4141 attggtgcgg ttatttgtgt gataggcatt cttccaaagt gcggcaagag gttattttta
     4201 attaaggcga ttacatcttt ttggtagttg tctgaaaaat tcttcttggt cttgcggtgt
     4261 tccagccacg cccacgctac attttcataa gtgttgctga tttcgttggc ctttgcctgt
     4321 tcctgttgtt ggcggtgggt ttgagggtcg atattttgag ccaataatgc cctatattct
     4381 tctctaattt ctctcgcttg tgctaaagaa atatcagggt aagccccaat gcttaaatca
     4441 ttccgctttt ttgtgaacgg tctttcatac ctaaaccgcc acaatttaga atttgaggta
     4501 ataacaagta gtaagccgtt accgtctctt aatgggctgt cttccggttt cgcttttctt
     4561 atttgggtat cgtttaacgg tgtgattttt ctagccatag taaaccttta ggagtataaa
     4621 gcctaaatct taggagtatg attttttgtt tgggagtaca aaactaaaag cgataaagcc
     4681 ttatttgaaa aggctctaaa gtggttttag gagtacgaat ttttgggggt attccggcgt
     4741 actcctaaat atactcctaa aatcttcgga tttgattgta tcgtataggt acgcaacgga
     4801 caataaaaaa gccttgaaac attgattttc aaggcttttt aggtatcggt tgaaccgtat
     4861 aggattataa tttggtggag ctggggggat ttgaaccccc gtccgaaatt tctctacctt
     4921 cagtactaca tgtttagtct agtctttaat ttcgcttatt cacagcggac agacacgcaa
     4981 tgaataaact agctttattt aatttagtgt ttcgatccta aagcggcggc gtccacacga
     5041 tctcgttttg ggtgacttat ctttgccctc gtcttacgag cggaagctga ggagataagg
     5101 ctttaaagca ggttattaag ctgctaaagc gtattgttcg tcgtttgcga ctattatttt
     5161 gcggtttgtt tacgaggcct accgcacctc gacatgcacc ttgggcttcg ccaatcccgt
     5221 cgaatccaaa atcagcccca aaagtagcgg tattctaaag gaaagcggtc gaatttgcaa
     5281 aaagttttgc aagtggcgga acttagcggt attgaatagc tcgaaataaa atcatacaaa
     5341 tcccacacca gatagattta actctgttat aatgtaaacc ttgctgagag tgaagagaaa
     5401 tagaatgaaa aaatttaacg agaacgagat tgaagatgct gaaattatag atgccgagcc
     5461 gattgatgtt gatgagatag acgaagaaga aacatcggta gaagcaatgc atccggcttt
     5521 ggtttccggc tcaatgcctg tgccgcaggg caatttggat agctacatcc gaatggcaaa
     5581 tcagtatccg attctcaccg ccgagcaaga aaaagaactg gcggagcgtt attattatga
     5641 tgaagacgtt gaggcggcaa aacagctgat cctttcccat atgcggtttg tgatccatat
     5701 tgctcgtggc tatttgggct atggtttacc gctggcagat ctgatccaag agggtaatat
     5761 cggcttgatg aaagcggtaa aacggtttga cccgaatgtc ggcgtgcggt tggtttcttt
     5821 tgcggttcat tgggtgaaag cggaaattca cgaatatgtg ctgaaaaact ggcgaattgt
     5881 gaaagtggca accaccaaag ctcaacgtaa attattcttt aatttacgca aaaataaaaa
     5941 ccgcttggcg tggtttaatg aagaagagat caaaaaagtt gccgatgatt taggggtttc
     6001 agtggaagaa gtacgagaaa tggaatctcg aatgaccgga caagatttag gttttgacct
     6061 gcctgccggt gatgacgatg aagacagcta cgctccttct atgtatattg aagatagcag
     6121 ctccaatttc gccgatgact tggaagacga acaacataat gggcaggcaa ccgctcaact
     6181 tgcctatgcg ttagccaccc ttgatgaacg tagccaagac atcatcaaaa cccgctggtt
     6241 agatgacaat aaagccacac tgcaagattt agccgataaa tataacatct cggcagagcg
     6301 ggtaagacag cttgaaaatg ccgcattgaa gaaaatcaag gaagcgattt cattagacgg
     6361 ctagaaatgc ctacaagcgg tgattttttt actcgctacg ctcgcacttc gtgccgtcgg
     6421 cagagccaac gttcaaacgc aagcgtttgt gcaaaatttt acgcaaaaag caccgcttgt
     6481 attttgacta aaaatgagga tactatgatt aacattaccg ataaacaaat tgacccaact
     6541 ttctaccagc gtctaccagc gagcagacgg ctttattaac gttgccaacg ctcatttaaa
     6601 aaacattgcc ccaaaccaag tcagcaatgc gatgttgttt ggttgtgcca gatttaacgc
     6661 ctatgttgcg gcaagcaagg cggaatataa acagcagttg gccgattcca gagaagaagt
     6721 tattcaatat tttgtggagc aatacaaaga aatgctcact gcaaatttag atgaatatat
     6781 ccaaaacttc gagcgatata ttgaagggaa aaaggcggat taaccgcctt tgttttgtgg
     6841 tcaaacttgt tttacaaaat cacttaaaac ttgattaatt ctactttgcc aacctttacc
     6901 tgaagctcta aaggcttcaa gaacgctttt atcaagacgt aacgaaacca attctttctt
     6961 tgaaccagct ggtctgccac gttttttcat tgcaattgaa aactctgatg gcgtaaccgg
     7021 gcgttcatcg tcatcttttc cgtcccaaat aaaatcttgg cttgccggaa tagcttgaat
     7081 agccataagt agctgttttt tctctaaatt tttattattc aaagtattgt tgtaagtatt
     7141 cataatatgc ctctctttct gctttagatg ctcgccgaaa gctaattact ctgatgtaat
     7201 cttccctttc tgtatatgca agtgataata cgagtaaacg tccaaatgaa tatgcaaaag
     7261 attgtattct aaattcattg ttacgaataa cttcaacatc aaatcgatag ggcgatgaga
     7321 gaacttcatc tgcatcggca aaatctaatc cgtgcttggc taaattgatt ttgcgtttag
     7381 tttcgtc
//
LOCUS       DANLUQ010000127         7285 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.128, whole genome shotgun sequence.
ACCESSION   DANLUQ010000127 DANLUQ010000000
VERSION     DANLUQ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7285
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.128"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            10..510
                     /locus_tag="PXB27_002004"
     CDS             10..510
                     /locus_tag="PXB27_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HDL5346759.1"
                     /translation="MQTINEIKEEIYPIFEPIIGTIRLKTYFSYYAVFKDGLMIALYQ
                     NGATYLRIAKKDIEYIRQYPKTCILCDEKIGIQSRKFHYIPQVLIDDTPKFSSLLFGI
                     IEELRTEKYKISQKRATQIRNLPNMNLKLERMLKKIGIHSIHDFTQEGYMSTFIKLIL
                     HRRLTV"
     gene            798..2192
                     /gene="fumC"
                     /locus_tag="PXB27_002005"
     CDS             798..2192
                     /gene="fumC"
                     /locus_tag="PXB27_002005"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005567406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HDL5346760.1"
                     /translation="MEFRIEKDTMGEVQVPADKYWAAQTERSRNNFKIGPEASMPAEI
                     IEAFGYLKKAAAYANHDLGVLAVEKRDLIAKACDEILANKLDDQFPLVIWQTGSGTQS
                     NMNVNEVVANRAHVLNGGKLGEKSIIHPNDDVNKSQSSNDTYPTAMHIAAYKKVVEHT
                     LPCVKRLQKTLAEKAEAFKDVVKIGRTHLMDATPLTLGQEFSAYAAQLAFGIKALENT
                     LPHLRELALGGTAVGTGLNTPQGYDVKVAEYIAKFTGLPFVTAQNKFEALATHDAIVE
                     THGALKQLAVSLYKIANDVRLLASGPRSGIGEILIPENEPGSSIMPGKVNPTQCEAMT
                     MVCAQVLGNDVTISFAGTQGHFQLNVYKPVMAYNFLQSAQLLGDACVSFDEHCAVGIE
                     PNYPRIKQQLDQSLMLVTALNTHIGYENAAKIAKTAHKNGTTLKEEAINLGLVTAEQF
                     DEWVRPEDMVGSLK"
     gene            2319..2429
                     /locus_tag="PXB27_002006"
     CDS             2319..2429
                     /locus_tag="PXB27_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005696979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDL5346761.1"
                     /translation="MKKLILIALVTTLGLSACGVKGPLYFPEQAPKHEQK"
     gene            2534..3784
                     /gene="lysA"
                     /locus_tag="PXB27_002007"
     CDS             2534..3784
                     /gene="lysA"
                     /locus_tag="PXB27_002007"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDL5346762.1"
                     /translation="MNHFEYKNNQLFAEEVAIADIIEQYGTPAYVYSRSTLERHWHAF
                     NNAFGSHPHLVCFAVKSNSNIALLNVMARLGSGFDIVSQGELERVLAAGGEASKVVFS
                     GVAKSHSEIKRALEVGIRCFNIESVSELHRINQVAGELGKIAPISLRVNPDVDAHTHP
                     YISTGLKENKFGVSVTQAREVYRLAKTLPNVKITGMDCHIGSQLTELQPFLDATDRLI
                     ILMEQLREDGIELHHLDLGGGLGVPYNGEEPPHPTEYAKALLEKLKGYENLEIIIEPG
                     RAITANAGILVTKVEYLKQNEDRNFAIVDTGMNDMIRPALYEAYMQITEVDKTLNRDK
                     AVYDVVGPICETSDFLGKQRELAISEGDLIAIRSAGAYGAAMSSTYNSRPQAAEIMVD
                     GNQAHLIKARATFADLWRLEKLLP"
     gene            3798..4427
                     /locus_tag="PXB27_002008"
     CDS             3798..4427
                     /locus_tag="PXB27_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HDL5346763.1"
                     /translation="MKLYGLTGACSFVPHVALEWVKLRANQDYAFQAVSREFIKSAEY
                     LALNPRGNVPLLVDGDLALTQNQAIVHYLDELYPEAKLFGSKTARDKAKAARWLAFFN
                     SDVHKSFVPLFRLPSYAEGNETLTKTIRQQSAEQILEQLAFANAHLENHIFFGEEISV
                     ADAYLYIMLNWCRLLGLDFSHLSQLSAFMQRVEADQGVDNVREQEGLKG"
     gene            4498..5943
                     /gene="tldD"
                     /locus_tag="PXB27_002009"
     CDS             4498..5943
                     /gene="tldD"
                     /locus_tag="PXB27_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HDL5346764.1"
                     /translation="MLNQVSNSLLAPSGLELSHLTQVLDHFSGRHIDYADLYFQLSQD
                     ESWSLEDSIIKEGGFYIDRGFGVRAISGEKTGFAYADQITLKQLEQCAAAARSISPTS
                     GQLSVKNFKKTTACSRYPAINPLETISREQKVELLHLVDKTARMADPRVIQVNANLSA
                     VYEEMLVAATDGTLAADIRPLVRLSVSVLVEQNGKRERGSAGAGGRFGLDWFLAPSET
                     GFSRAESLTKEAVRQALVNLTAIPAPAGTMPIVLGAGWPGILLHEAVGHGLEGDFNRK
                     ESSLFSGKIGQMVTSELCTIVDDGTVPNMRGSITVDDEGVPSQRNVLIENGILKGYMQ
                     DKLNARLMGVAPTGNGRRESYAHLPMPRMTNTYLTEGNHEFEEMIESVEYGLYAPHFS
                     GGQVDITSGKFVFSTAEAYLIEKGKITKPVTGATLIGSGIEAMQQVSMVGKKMELDPG
                     IGTCGKEGQSVPVGVGQPTVKLDKITVGGRG"
     gene            6007..6888
                     /gene="prmA"
                     /locus_tag="PXB27_002010"
     CDS             6007..6888
                     /gene="prmA"
                     /locus_tag="PXB27_002010"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDL5346765.1"
                     /translation="MAWVQIRLNSTDKQAEEISDFLTEIGAVSVTFMDSQDTPIFEPL
                     PGETRLWGNTDVVGLFDAETDMNLIVEALKTSKLVDENFAHKIEQIEDKDWEREWMDN
                     FHPMQFGKRLWICPSWREVPDPNAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLEGK
                     TVIDFGCGSGILAIAALKLGAKQAIGIDIDPQAILASQNNAEANGVAEKLQLFLAKDQ
                     PRELQADVVVANILAGPLKELAPNIITLVKPNGNLGLSGILATQAESVCEAYSEAFTL
                     DPVVEKEEWCRITGRKI"
     gene            complement(7063..7138)
                     /locus_tag="PXB27_002011"
     tRNA            complement(7063..7138)
                     /locus_tag="PXB27_002011"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7103..7105),aa:Trp,seq:cca)
     gene            complement(7204..7280)
                     /locus_tag="PXB27_002012"
     tRNA            complement(7204..7280)
                     /locus_tag="PXB27_002012"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7244..7246),aa:Asp,seq:gtc)
BASE COUNT         2174 a         1624 c         1619 g         1868 t
ORIGIN      
        1 agtacatata tgcaaacaat caatgaaatt aaagaagaaa tctaccctat atttgagcct
       61 atcattggaa caattcggct aaaaacctac ttttcctact acgcagtttt taaggatgga
      121 ttgatgattg cattgtatca aaatggagca acttatttac gtattgcgaa aaaagatatt
      181 gaatacattc gtcagtatcc taaaacctgt atcttatgcg atgaaaaaat tggtatacaa
      241 agtagaaaat ttcattatat tccgcaagtt ctcatagatg atacaccaaa atttagctcg
      301 ctactctttg gaattataga ggaactccga acggagaagt acaaaataag ccaaaagcga
      361 gctacacaaa tccgaaacct accaaatatg aatttaaagt tagaaagaat gttgaaaaaa
      421 atagggattc attccattca tgattttact caagaagggt atatgtcaac ttttatcaaa
      481 ctgattctcc accgaagact tactgtttaa gctaaacgga gctttaaatc accaatatat
      541 ttacaccttc acagatcaac aaaaaaggga actaatgcaa gaagcaaatc aagcgctata
      601 taattcaggc ctaagaaaaa gatttaacgc cctgtagtta aataatgtga tccaaatcat
      661 aaaatataaa aaaaccaata aggaattgca attcgcagtt cctttctttt tcttttatat
      721 gctagtatca ccttatacct ctaaattatt agtgcctact gattttcaat taatccaaca
      781 atttaggaga gcgcatcatg gaattccgta tcgaaaaaga cacaatgggc gaagtgcaag
      841 ttcctgccga taaatactgg gcggcgcaaa ccgagcgttc acgcaataac ttcaaaatcg
      901 ggccggaggc atcaatgccg gcggaaatta tcgaggcgtt tggctatctg aaaaaagcgg
      961 ctgcctatgc taatcacgat ttaggcgtac tagcggtcga aaaacgggat ttaattgcaa
     1021 aagcctgtga tgaaattttg gcgaacaaac tcgatgatca attcccgctg gtgatttggc
     1081 aaaccggctc gggtacgcag tcaaatatga acgtgaatga agtcgtggca aaccgtgcac
     1141 acgtgttaaa cggcggcaag ctgggtgaaa aatcgattat tcacccgaat gatgatgtga
     1201 ataaatccca atcttctaac gacacctacc caactgcaat gcacatcgct gcttacaaaa
     1261 aagtggtgga acacacccta ccttgtgtga aacgtctgca aaaaacctta gcggaaaaag
     1321 ccgaagcctt taaagatgtg gtcaaaatag ggcgtactca cttaatggat gctaccccgc
     1381 tcacgctcgg tcaagagttc tctgcctatg cagcacagtt agcattcggc atcaaagcat
     1441 tggaaaacac cctgccacac ctgcgtgagc tggcactcgg cggcacagca gtcggcaccg
     1501 gcttgaatac cccgcaaggt tacgatgtga aagtggcgga atacattgcg aaattcaccg
     1561 gtttaccgtt cgttaccgcc caaaacaaat ttgaggcatt agcgactcac gatgcgattg
     1621 tcgaaaccca cggtgcatta aaacagcttg cggtgtcgct ctacaaaatt gccaacgatg
     1681 tccgtttatt agcctccggt cctcgttcag gcattggcga aattctgatt ccggaaaacg
     1741 agccgggttc atcaattatg ccgggtaaag taaacccaac ccaatgcgaa gcaatgacaa
     1801 tggtatgtgc ccaagtatta ggcaacgatg taaccatctc ttttgccggc acgcaagggc
     1861 atttccaact gaatgtctat aaaccggtga tggcgtacaa cttcctgcaa tcggctcaac
     1921 tgctaggcga tgcgtgcgtg tcgtttgacg aacactgtgc agtcggcatt gaaccgaact
     1981 accctcgcat taaacaacag ctcgaccaat cgctgatgtt agtgaccgca ttaaatactc
     2041 acattggtta tgaaaatgca gcgaaaatcg ccaaaactgc ccacaaaaac ggcacaacac
     2101 tgaaagaaga ggcgattaac ttaggcttag tcacggcaga acagttcgat gaatgggttc
     2161 gtccggaaga tatggttggc agcttgaaat aagcccaacc cttctcttgt aatctgattt
     2221 acacctcaat ctaccggtaa gattgaggtg taatttttta tagcgagggt actgattttg
     2281 ttttagaatg aagcatcatt ttttaagcgg aaaaacctat gaaaaaactc attttaatcg
     2341 cattagttac tactttaggc ttaagtgctt gtggggtcaa aggcccactc tacttccctg
     2401 agcaagcccc taaacatgaa caaaaataag cattgcaagc ggtctgtttt ggctaaaatt
     2461 ttgcaaattt ttgtgaaaaa atgaccgctt gttgcctctt ttctttcaaa atcccaaaca
     2521 caacggactt tcaatgaacc attttgaata taaaaacaac caactttttg ctgaagaagt
     2581 cgctattgcc gatattatcg agcaatacgg cacgccagct tatgtttact ccagatctac
     2641 ccttgaacgc cactggcacg cattcaataa tgcgtttggc tcgcacccac atttagtttg
     2701 ctttgcagtc aaatccaact ctaacattgc cttgttaaat gtaatggctc gtctcggttc
     2761 aggctttgat attgtctctc aaggcgagtt ggagcgtgtc ttagctgccg gcggcgaagc
     2821 ctctaaagtt gtcttttccg gtgtggcaaa atcgcacagc gaaatcaaac gagcattgga
     2881 agtcgggatt cgttgcttta acattgaatc ggtttccgaa cttcaccgca ttaaccaagt
     2941 tgccggtgaa ctaggcaaaa tcgcaccgat ttcactgcgg gtaaacccgg atgtggacgc
     3001 ccacactcac ccatacattt ccaccggttt aaaagaaaac aaattcggcg tgagcgttac
     3061 tcaagcccgt gaagtgtatc gcttagccaa aaccttaccg aatgtgaaaa ttaccggtat
     3121 ggattgtcac atcggttcgc aattaaccga attgcaacct tttttagatg caacggatcg
     3181 cttaatcatc ttaatggagc aactgcgtga agacggcatt gagctacacc acttagactt
     3241 aggtggcggt ttaggcgtgc cttacaacgg cgaagagcct ccgcatccga ccgaatatgc
     3301 caaagccttg ctggaaaagc tcaaaggcta tgaaaactta gaaatcatta ttgaaccggg
     3361 gcgtgccatt accgcaaatg ccggtatttt agtgacgaag gtggaatact tgaaacaaaa
     3421 tgaagatcgt aattttgcga ttgtcgatac cggtatgaac gatatgattc gcccggctct
     3481 gtatgaagcc tatatgcaaa ttacggaagt ggataaaacc ttaaaccgtg acaaagctgt
     3541 ttacgatgtg gtcggtccga tttgcgaaac ctcggatttc ttaggcaaac aaagagaatt
     3601 ggcaatttct gaaggggatt taatcgctat tcgttctgcc ggtgcttatg gggcggcgat
     3661 gtcttcaacc tacaactctc gcccacaagc ggcagaaatt atggttgatg gcaaccaagc
     3721 ccatttaatt aaagcccgag ccacttttgc tgatttatgg cgtttagaaa aattattacc
     3781 ttaatgagga agaaaagatg aaattatatg gattaaccgg tgcttgttca tttgtaccac
     3841 acgttgccct agagtgggtg aaattgcgtg ccaatcaaga ttatgcgttt caagctgtta
     3901 gccgtgaatt tattaaatcg gcggaatatc tggctttaaa ccctcgtggt aacgtaccat
     3961 tgttggttga tggcgacttg gccctcacgc aaaaccaagc tatcgtccac tatttagacg
     4021 agctttatcc ggaagctaaa ttattcggca gcaaaaccgc tcgtgataaa gccaaagcgg
     4081 ctcgttggtt agcttttttc aattcagatg tgcataaatc attcgtgcca ctgttccgct
     4141 tgccaagtta tgcggaaggc aatgaaacgc tcaccaaaac tattcgccaa caatcggctg
     4201 agcaaatttt agaacaactg gcttttgcga atgctcattt agaaaatcat attttctttg
     4261 gcgaagaaat ttcggttgcc gatgcgtatc tctacattat gctcaactgg tgtcgcctac
     4321 tagggttgga tttctctcat ctttcccaac tttctgcctt tatgcaacga gtggaggcag
     4381 accaaggcgt agataatgtg cgtgagcaag aaggcttaaa aggttaattc accattgttc
     4441 atttaagacg gtagatttta tgttaaaatc taccgtcttt tatttttagg aatcaccatg
     4501 ctaaatcagg tttcaaacag cttgcttgct ccaagtgggc ttgaattatc gcacttaact
     4561 caagtgttag atcacttctc cggccgtcat attgattatg ccgatctcta cttccaactc
     4621 agccaagacg aaagctggag cttggaagac agcatcatca aagaaggcgg tttttatatc
     4681 gaccgagggt tcggtgtgcg agccatatca ggcgaaaaaa ccggctttgc ctacgccgat
     4741 caaatcacgt taaaacagct tgagcaatgt gccgctgccg cacgttcaat cagcccaaca
     4801 agcggtcaat tatcggtaaa aaatttcaaa aaaacgaccg cttgttctcg ctacccggca
     4861 atcaacccgc tagagaccat cagccgtgaa caaaaggtcg aattgctcca tttggtggat
     4921 aaaaccgctc gaatggcaga tccgagagtg attcaagtga atgcgaatct ctcagcggta
     4981 tatgaagaaa tgttagtggc agcgactgac ggcacactgg ctgcggatat tcgcccgtta
     5041 gtgcgtttat ctgtgtcggt actggtggaa caaaatggca agcgtgaacg aggttcagca
     5101 ggtgccggtg gtcgctttgg tttagattgg ttcttagcac caagcgaaac cggctttagc
     5161 cgtgcggaat cgcttaccaa agaagccgtt cgccaagccc tcgttaatct cacagccatt
     5221 cctgcaccgg caggcacgat gccgattgtt ctcggtgctg gctggccggg cattttgctc
     5281 cacgaagcag tcggacacgg cttggagggt gattttaacc gtaaggaatc ttcgctcttc
     5341 agtggtaaaa tcgggcaaat ggtcacctct gagctttgca ccattgttga tgacggtacg
     5401 gtgccgaata tgcgaggctc aatcacggta gatgacgaag gcgtgccttc gcaacgtaat
     5461 gtgttaattg aaaacggcat tctaaaaggc tatatgcaag acaaactcaa tgcccgcctg
     5521 atgggagttg ccccaaccgg caacggcaga cgggaatctt acgctcattt gccgatgcca
     5581 cgaatgacca acacctacct caccgaaggc aatcacgaat ttgaagagat gattgaatcg
     5641 gtcgaatacg gtttatatgc tcctcacttc agtggtggac aagtggatat tacctccggc
     5701 aaatttgtgt tctccaccgc cgaagcctac ttaatcgaaa aaggcaaaat caccaaacct
     5761 gttaccggtg caaccctgat cggcagcggc atagaagcaa tgcaacaagt ttcaatggtc
     5821 ggcaaaaaaa tggaactcga ccccggcatc ggcacttgtg gcaaagaagg gcaatccgta
     5881 ccagtcggcg tagggcagcc tacagtgaag ttggataaaa ttacggtggg gggaagaggg
     5941 taatctctcc ccgtttgcaa aatataaaga aaatatcacc gcttgtagcg ataaaaggaa
     6001 aacaaaatgg cttgggtaca aattcgttta aacagtacag ataaacaggc tgaggaaatc
     6061 agcgattttt taaccgagat tggagcggtg tcagtgactt ttatggatag tcaagatacc
     6121 ccaatttttg aacctcttcc gggggaaacc cgtctttggg gaaataccga tgtggttggg
     6181 cttttcgatg cggaaactga tatgaatctt atcgtagaag cactaaaaac cagcaaattg
     6241 gtggacgaaa actttgctca caaaatcgag caaatcgaag acaaagactg ggaacgggag
     6301 tggatggata atttccaccc aatgcagttt ggcaaacggt tatggatctg cccaagctgg
     6361 cgtgaagtac cagacccgaa tgcagtgaat gttatgttag accctggttt agcatttggc
     6421 accggcacac acccaacgac ctcgctttgc ttacaatggt tagatagctt agatttagaa
     6481 ggcaaaacgg ttatcgactt cggctgtggc tccggtattt tagctatcgc tgcacttaaa
     6541 ctcggtgcaa aacaagccat cgggattgat atcgacccac aggcgatttt agctagtcaa
     6601 aacaatgctg aggcgaacgg cgtagcagaa aaactacaac tattcttagc caaagatcag
     6661 ccaagagagt tacaagccga tgtcgttgtt gcgaatattc tagccggccc actcaaagag
     6721 ttagcaccga atatcatcac gctggttaaa ccaaacggca atttaggctt atccggcatt
     6781 cttgctactc aggctgaaag tgtgtgtgag gcttatagcg aagcctttac gctagaccct
     6841 gttgtagaaa aagaagaatg gtgtcgtatt acaggccgca aaatctaata taagaagccc
     6901 tgttttaaac agggcttact ttttgataaa ttctactact ctttacctgt cttgggaatc
     6961 gttctcccta aattaattaa cacaagcggt agaaataaca ggaaaattta caaattacat
     7021 aaatgaaaaa agccgactga aatcagtcgg ctttctaaat aatggcaggg gcggagaggc
     7081 tcgaactccc aacacccggt tttggagacc ggtgctctac caattgaact acgcccctat
     7141 tgataggtat tcattttgca cttatagggg cgaattacta ttcgcccctg cattaaagat
     7201 tattggcgga acggacggga ctcgaacccg cgaccccctg cgtgacaggc aggtattcta
     7261 accagctgaa ctaccgctcc taaat
//
LOCUS       DANLUQ010000128         7257 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.129, whole genome shotgun sequence.
ACCESSION   DANLUQ010000128 DANLUQ010000000
VERSION     DANLUQ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7257
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.129"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            9..995
                     /gene="cas1c"
                     /locus_tag="PXB27_002013"
     CDS             9..995
                     /gene="cas1c"
                     /locus_tag="PXB27_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014167737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1"
                     /protein_id="HDL5346766.1"
                     /translation="MTTQGSYLHKERETLVVEQDRKKVAQLPIHSIGHIFCFGNVLVS
                     PFLMGFCGENNVNLAFFTENGRYLGRLQGRQSGNVLLRRAQYKKSETNPVPVARNIIA
                     AKIQASKRVLQRQIRNHGENADLQSTITALNYSLQQLKTADNLDLIRGIEGDAAARYF
                     GVFSHLIKENSGFHFDGRNRRPPRDGVNALLSFLYSIVGKDISGALQGVGLDPQIGFL
                     HADRSGRDSLAQDILEEFRAWWVDRMVLSLINRGQVKPDDFVTEASGAVTLKPEARKL
                     LFQTLQAKKQEKIIHPFLEEEVEIGLLPYIQAMLLARHLRGDLAEYPPFLMR"
     gene            1062..1355
                     /gene="cas2"
                     /locus_tag="PXB27_002014"
     CDS             1062..1355
                     /gene="cas2"
                     /locus_tag="PXB27_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005540845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HDL5346767.1"
                     /translation="MLMLITYDISFDDPEGQARLRRIAKHCLDYGVRAQYSVFECDVT
                     PAQWVTLKNKLLATYNPDCDSLRFYHLGSKWRNKVEHHGAKPSVDIFKDTLIL"
     repeat_region   1547..2582
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1547..1566
                     /rpt_unit_seq="cagccacctacgcgtggctg"
     gene            2832..4307
                     /gene="rng"
                     /locus_tag="PXB27_002015"
     CDS             2832..4307
                     /gene="rng"
                     /locus_tag="PXB27_002015"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005610087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HDL5346768.1"
                     /translation="MSSTELLVNVTPSETRVALVENGILKELHIEREAKRGIVGNIYK
                     GRVTRVLPGMQSAFVDIGLEKAAFLHASDIVSHTECVDENEKKQFLVKDISELVREGQ
                     DIVVQVVKDPIGTKGARLTTDITLPSRYLVFMPENSHVGVSQRIESEEERARLKSLVE
                     PYCDELGGFIVRTAAEEATEESLKQDVEFLKRLWRKVLERKGKYSAKSMLYGELALAQ
                     RVLRDFIGTHINSVRIDSKLTYEQVREFLAEFMPELTDCVTLYTGQQTLFDAYGVEKA
                     IQKALDKRVDLKSGGYLIIEQTEAMTTIDINTGAFVGHRNLAHTIFNTNIEAIQAIAH
                     QLQLRNLGGIIIIDFIDMQEEEHRERVLQSLQEALKGDRVKTNVNGFTQLGLVEMTRK
                     RTRESLERILCCDCQACQGRGTVKTVETVCYEIMREIVRVHHLFKTENIHVYAAKDVA
                     EYLINEESHALLAELQAFIGKKIEIKLEPYYHQEQFDVVVM"
     gene            4461..5963
                     /gene="lysS"
                     /locus_tag="PXB27_002016"
     CDS             4461..5963
                     /gene="lysS"
                     /locus_tag="PXB27_002016"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDL5346769.1"
                     /translation="MSEVENQELDLNGEMLARREKLNKIREQGNAFPNTFRRDALAKD
                     LHAQYDAVEGETLKEQNIQVKVAGRIMLKRVMGKASFFTIQDVSGAIQLYVARDNLSD
                     SVYEEKVSLWDLGDIVGVEGALFKTKTGELTVRCSEVQLLTKSLRPLPDKHHGLTDLE
                     TRYRQRYLDLISNEESRRTFMIRSQVVSGIRKFFLERDFIEVETPMLQVIPGGAAAKP
                     FITHHNALDVDMYLRIAPELYLKRLVVGGFERVFELNRNFRNEGVSVRHNPEFTMIEY
                     YQAYADYHDLMDNTEELLRQLALDILGTTDVPYGDYVFDFGKPFERITMHDAIVKYGN
                     GIQREDLDSFEKSVEIAKGLGIEIQKSWGLGSVVNAIFEEVAEHQLIQPTFLMAHPAE
                     ISPLARRNDENPEVTDRFELFIGGREIGNGFSELNDAEDQAERFDAQVAAKDAGDDEA
                     MFKDEDFIVALEHGLPPTAGEGLGIDRLVMIFANAPSIRDVILFPAMRQK"
     gene            complement(6041..7114)
                     /locus_tag="PXB27_002017"
     CDS             complement(6041..7114)
                     /locus_tag="PXB27_002017"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HDL5346770.1"
                     /translation="MAQQITLFEDVPLPLTLGGELSPITVAYQTYGRLNAEKSNAVLL
                     CHALTGDAKPYYDSPTEKGWWQDFIGKGLAFDTDKYFFICSNVLGGCKGTTGPASINP
                     KTNKPYGSLFPIITVQDIVNVQKALLDYLEIPQLHAVVGGSFGGMQATQWGISYPDKV
                     RNIINLCSSLTLSAEAIGFNHVMRQAIINDPHFNGGNYYDGIAPDNGLKIARMLGMLT
                     YRTDIQLAKAFGRETKQQGQIWGDHFQVESYLSYQGTKFLGRFDANTYLRLLRALDLY
                     DPALGYENEETALKRIKANYTFVAVTSDQLFKQVDVYKSKQKLEQAGVNLEFHEFHSD
                     FGHDAFLVDYDFFEPMIKAGLAK"
BASE COUNT         2110 a         1350 c         1671 g         2126 t
ORIGIN      
        1 ccctctacat taccacccaa ggtagctatt tgcacaaaga gcgagaaacc ttggtggtgg
       61 agcaagatcg gaagaaagtg gcacagttgc cgattcattc cattgggcat attttttgtt
      121 ttggcaatgt gttagtgtcg ccgtttttaa tgggattttg tggcgaaaat aacgtaaatt
      181 tagcgttttt taccgaaaat gggcgttatc ttgggcggtt gcaggggcgg caaagtggta
      241 atgtattgtt acgccgagcc cagtataaaa agtcggagac caaccctgtt cctgtggctc
      301 gcaacatcat tgcagccaaa attcaagcat ctaagcgggt gttacaacgg caaattcgta
      361 atcacggcga aaatgctgac cttcaatcga caattaccgc attaaattac agcctgcaac
      421 agttgaaaac agcagacaat ttggatttga ttcgtggcat tgagggcgat gctgcggctc
      481 gttattttgg ggtgttttct catcttatca aagaaaatag cggtttccac tttgacggac
      541 gtaatcgccg cccacctcgt gacggtgtga atgcgttgct ttcatttctt tatagcattg
      601 tagggaaaga tattagcggg gcattgcaag gcgtggggct tgatccccag attggctttt
      661 tgcatgcaga tcgttcgggg cgagatagct tagctcaaga tattttggag gaatttcgag
      721 cgtggtgggt ggacagaatg gtgctgtcgc tgatcaatcg agggcaggtt aagcctgatg
      781 attttgtgac ggaagccagt ggtgcggtaa cgctcaagcc tgaagccaga aaattactat
      841 ttcaaacctt gcaagcgaaa aaacaagaaa aaatcatcca cccattttta gaggaagaag
      901 tggaaatcgg cttgttacct tatatccaag cgatgttatt ggctcgtcat ttgcgtggcg
      961 atttggcaga atatccgccg tttttaatgc gttaaaatct gcaaaatttt gttgaaattt
     1021 gaccgcttgt ttatgtttta ttcattgagg aattagcaac tatgctgatg ttgattacct
     1081 acgacatttc atttgatgac cccgaagggc aggctcgcct acgccgtatt gctaagcatt
     1141 gtttggatta tggtgtgcgg gcgcaatatt ctgtgtttga gtgtgatgtt actcctgctc
     1201 agtgggtaac attgaaaaat aaattattgg caacttacaa cccagactgt gatagtttgc
     1261 gcttttacca tttaggcagt aaatggagga ataaagttga gcaccatggc gcaaaacctt
     1321 cagtggatat ttttaaagat acgctgattt tataaatcgc tagcctgtag ttctcataaa
     1381 aatgtagcaa ggttagcgaa tctatattgt tctttaataa tcaaggaatt agaaaaaata
     1441 aggcacaata tattggcgat atgctatact ttatccactc tctacaaaac gttagcgaat
     1501 aaagggctaa aaagcctgat atttcaatac gttatcttat agggaccagc cacctacgcg
     1561 tggctgcgaa ttgaaaccct ataacgagca atggcatttt gatctacggt aaaccagcca
     1621 cctacgcgtg gctgcgaatt gaaacttctt gaatacctcc ggagtcagtt aagataaggc
     1681 cagccaccta cgcgtggctg cgaattgaaa ccaccgctgc tacttcggtt atcggagtag
     1741 tttgcaccca gccacctacg cgtggctgcg aattgaaacg tggcacagta acacgagcag
     1801 atgctgataa acgcccagcc acctacgcgt ggctgcgaat tgaaaccagc agccgttgtt
     1861 accgtggtgc aatgcttgcg ccagccacct acgcgtggct gcgaattgaa actacactta
     1921 ttgccatagg gtagtagtaa tcttttaccc agccacctac gcgtggctgc gaattgaaac
     1981 tgcgattttt ggatttttcg ggcgataaat gataccagcc acctacgcgt ggctgtgaat
     2041 tgaaactcgt ggctggttat acaaagaaaa gcattgttgg gcagccacct acgcgtggct
     2101 gtgaattgaa acttaaatct aaagctaaag ctattttatt aattaaagca gccacctacg
     2161 cgtggctgtg aattgaaact ttaccgttta ccgattggat tttaatgcta gaggcagcca
     2221 cctacgcgtg gctgtgaatt gaaaccctaa attgccgtaa aaatctgcca ttagctacgc
     2281 gcagccacct acgcgtggct gtgaattgaa acctcataac gaatccaatc ctgttcggtt
     2341 aatccccgca gccacctacg cgtggctgtg aattgaaaca ggggcgtgta ccgcaatcaa
     2401 ttttaattat aaccaagcag ccacctacgc gtggctgtga attgaaactt tatctgttgc
     2461 ggtcaattgt atatcttgaa tttgcagcca cctacgcgtg gctgtgaatt gaaacctcgt
     2521 cttgaattgg aaaatcaagg gcgacaatag gcagccacct tcgcgtggct gtgtattgaa
     2581 acggtgtacc cacaaatcct atgttttcgc cattggctgc taccttcgca tcaatatata
     2641 ttgaaatatc aatggcaaat ttgtatgttg ttaattagac tgattgcatc tgctatgtat
     2701 catagtaatg acgaaaatat tctattttgc aaaaaataat cagaaattac ccgcttgttc
     2761 gaactgagtt tttgttttct aagatgtgaa atgatacact ttagatagtt ttaaaatata
     2821 gagacagtaa gatgagtagt acggaattat tagtgaatgt taccccaagc gaaacaaggg
     2881 tagcgttggt agaaaacgga atattgaaag aattacatat tgagcgggaa gctaaacgcg
     2941 ggattgttgg taatatttat aagggaaggg tgacaagggt attaccaggt atgcaatctg
     3001 cttttgtgga tatcggcttg gagaaggctg cttttttgca cgcttctgac atcgtttcgc
     3061 atacggaatg tgttgatgaa aatgaaaaga agcaatttct ggttaaagat atttctgaat
     3121 tggtgcgtga agggcaagat atagttgtac aagtagtgaa agatccaatt ggtactaaag
     3181 gagctaggct cactactgat attacgttac catctcgtta tttagtgttt atgccagaaa
     3241 atagccatgt gggagtatct cagcggattg aaagtgaaga agagcgtgct cgtttaaaaa
     3301 gcttggttga gccttattgt gatgaacttg ggggctttat tgtacgtacc gctgcagaag
     3361 aggcaactga agaaagttta aagcaagatg ttgaattttt aaaacggttg tggcgtaaag
     3421 tattagaacg aaaagggaaa tattccgcaa aatcaatgct ttatggagag cttgcgcttg
     3481 ctcaaagggt actgcgtgat tttattggta cacatatcaa ttcggttaga atcgattcaa
     3541 agctaactta tgagcaagtg cgagaatttt tagctgagtt tatgccagaa ttaacggatt
     3601 gtgtcaccct ctataccggt cagcaaactt tatttgatgc ctatggtgta gaaaaggcaa
     3661 tccaaaaggc attggataaa cgggttgatt taaaatcggg gggctattta attattgagc
     3721 aaactgaggc gatgacgact attgatatta atactggtgc ttttgtcgga catcgtaacc
     3781 ttgcacatac gattttcaac acaaatattg aagcaataca ggcaattgca caccagctac
     3841 agttgcgtaa tttaggtggt attatcatta ttgattttat tgatatgcaa gaggaagaac
     3901 atcgtgagcg cgttttacaa tcgttacaag aggcattaaa aggcgatcgt gttaaaacca
     3961 atgtgaatgg ctttactcag cttggcttgg tggagatgac cagaaaacgt acaagggaga
     4021 gtctagagcg aattctttgt tgcgattgcc aagcttgcca agggcgtggc acagtgaaaa
     4081 ctgtagagac ggtttgctat gaaattatgc gtgaaattgt gcgtgtacat catttgttta
     4141 agacagaaaa tattcacgtt tatgcagcta aagatgttgc tgaatattta attaacgagg
     4201 aaagccacgc attattggct gagcttcagg cgtttatcgg taaaaaaatt gagattaaac
     4261 ttgagccata ttatcatcaa gagcagtttg atgtggtcgt gatgtaatct attacttggt
     4321 tgaggctaaa tagttagcct caatatcgtt agttacaaaa aatttagaaa atctgaccgc
     4381 ttgtttccta tccaaattcc tcaaaataac gtataatcgt ccagttttat ttatcctttt
     4441 ttgaaatcta aggtaattca atgtctgaag tagaaaatca agaattagat ctaaacggtg
     4501 aaatgttagc ccgccgtgaa aaattaaaca aaatccgtga gcagggtaac gcatttccaa
     4561 atacattccg tcgtgatgcg ttagccaaag atctgcacgc tcaatatgat gcagtggaag
     4621 gtgaaacctt aaaagaacag aatattcaag tgaaagttgc cggtcgtatt atgttaaaac
     4681 gagtaatggg taaggcatct ttctttacca ttcaagatgt gagcggtgcg attcagcttt
     4741 atgtagctcg tgataactta agtgacagcg tttacgaaga aaaagtcagc ctttgggatt
     4801 taggcgacat tgtgggcgtc gaaggtgcgt tatttaaaac caaaaccggt gaattaacgg
     4861 ttcgttgctc tgaagttcaa cttttaacca agtctttacg cccgttgccg gataaacatc
     4921 acggtttaac ggatttagaa actcgttatc gtcagcgtta tttagacttg atttctaatg
     4981 aagaatctcg ccgtactttt atgatccgct cgcaagtagt ttcaggcatt cgcaaattct
     5041 tcttagaaag agactttatt gaagttgaaa cgccaatgtt gcaagtgatc ccgggcggtg
     5101 cggcagcgaa accgtttatc actcatcata atgcgttaga tgtggatatg tatctgcgta
     5161 ttgctccaga gctttactta aaacgtttag ttgtgggtgg ttttgagcga gtatttgagc
     5221 ttaaccgtaa cttccgtaac gaaggggttt cagtacgcca caatcctgaa tttacaatga
     5281 ttgagtacta ccaagcctat gcggattatc acgatttaat ggataatacc gaagaactgt
     5341 tacgccaatt agccttagat attcttggca caactgacgt accttatggt gattatgtat
     5401 tcgatttcgg taaaccgttt gagcgtatta cgatgcacga tgcgattgtg aaatatggta
     5461 acggcattca acgtgaagat ttagacagct tcgagaaaag tgtggaaatt gcgaaaggct
     5521 taggcattga aattcaaaaa tcttggggtt taggctcggt agtcaatgcg atttttgaag
     5581 aagtggcaga acatcaatta attcagccaa ctttcttgat ggctcacccg gcagaaattt
     5641 caccattagc tcgccgtaat gatgaaaacc cagaggtaac tgatcgtttt gagttattta
     5701 tcggtggtcg tgaaattggt aatggtttct cagaattaaa cgatgcagaa gaccaagcag
     5761 agcgttttga tgctcaagtt gcggcgaaag atgccggtga tgatgaagca atgtttaaag
     5821 atgaagactt tatcgtggca ttggaacacg gtctgccgcc aacagcgggt gaaggtttag
     5881 gaattgaccg cttagtaatg attttcgcta acgccccatc aatccgtgat gtaattttat
     5941 tcccagcgat gcgtcagaag taatcagaaa tagaacaaaa gcacacttcg gtgtgctttt
     6001 ttgtattaca agcggtcaaa tttttaataa aaaatgcaat ttattttgct aatccggctt
     6061 taatcatcgg ttcaaaaaag tcataatcaa ccaaaaaggc atcgtgtccg aaatcggagt
     6121 gaaactcgtg gaactccaaa tttacgcctg cctgttctaa tttttgtttg ctcttataca
     6181 cgtcaacttg cttgaatagt tggtcgcttg ttaccgctac aaaagtgtag ttggctttaa
     6241 tacgttttag ggcggtttct tcattttcat agccaagagc cgggtcgtat aaatctaatg
     6301 ctcggagtaa acggaggtaa gtgttggcat cgaaacgacc taaaaatttc gtgccttgat
     6361 agcttaaata ggattccact tggaaatggt cgccccaaat ttgtccttgc tgtttggttt
     6421 ctcgtccaaa ggctttagca agttgaatat cagtacggta agtcagcata ccgagcatac
     6481 gggcgatttt taagccgtta tcaggtgcaa tgccatcata atagttccca ccgttaaagt
     6541 gtggatcgtt aataatagct tggcgcataa cgtggttaaa accgatagcc tcggcactta
     6601 gggtaagtga cgaacaaaga tttataatgt tgcgaacttt atcagggtaa ctaatacccc
     6661 actgtgttgc ttgcattccg ccgaaagatc cccctaccac tgcgtgaagt tgagggattt
     6721 ctaaatagtc caataatgct ttttgtacat taacaatatc ttgcactgta ataattggaa
     6781 acaaactacc gtaaggttta ttggttttag ggttaatcga agctgggccg gttgtgcctt
     6841 tacaaccgcc taatacatta ctgcaaataa agaaatattt atcggtatca aacgcaagcc
     6901 ctttgccgat gaaatcttgc caccagcctt tctcggttgg tgagtcatag taaggcttag
     6961 catcaccggt aagggcgtgg caaagcaaga ccgcattgct tttctctgca tttaatctgc
     7021 cgtaagtctg gtaagccact gtgatagggg acagttcgcc tcctaatgtg agtggtaaag
     7081 gtacgtcctc aaaaagggta atctgctgag ccataaaaat cctgtttgca aaaaattctg
     7141 cttttttgac cgcttgttgt aatgaccgtt aaattagcgg tgttcatttc aattgtcaag
     7201 aatttttaga cgtctaaacg tctagatttt taaacggcta aaatcaaaat tttattg
//
LOCUS       DANLUQ010000129         7115 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.130, whole genome shotgun sequence.
ACCESSION   DANLUQ010000129 DANLUQ010000000
VERSION     DANLUQ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7115
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.130"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            80..1171
                     /gene="ulaG"
                     /locus_tag="PXB27_002018"
     CDS             80..1171
                     /gene="ulaG"
                     /locus_tag="PXB27_002018"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HDL5346771.1"
                     /translation="MSKIQEITRESWILSTFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     CVGVWIKTPAGANICMDLWCGRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRAQPMVL
                     DPFAINEVDFILASHYHSDHIDVNVAAAICNNPKLDHVKFVGPWHCAELWKKWGVPEE
                     RIIVVKPGDVVKLKDVEIHALDSFDRTCLVTLPVEGAEERGGELAGLCPSDEEMGRKA
                     VNYVFKTPGGTIYHGADSHYSIQFAKHGKQFDIDVALNNYGENPVGIADKMTSVDLLR
                     MAECLRCKVIIPVHHDIWTNFMASTQEIIDLWRMRKDRLQYQFHPFIWEVGGKYVYPR
                     DKDLIEYHHPRGFDDCFEQEPNIQFKSML"
     gene            1255..2001
                     /gene="ulaR"
                     /locus_tag="PXB27_002019"
     CDS             1255..2001
                     /gene="ulaR"
                     /locus_tag="PXB27_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HDL5346772.1"
                     /translation="MNESYRHKELLALLEERKALSTADIMQLLNISPATARRDISKLD
                     GQGKLKKVRNGAEAILAPTFLQHTKITNNAQEKRRIAEAAGRLCKDGESLILTCGSTM
                     EMLGEVLCGRKLQIITNYLPLANQLIAGCHDDVVIMGGQYNKHKKVMLSINTQNEMTY
                     AATTMFTSGKGLTSEGLFKTDMIIANAEQQMAKHAERYVVLLDSTKLGKKVGMLFKEF
                     DKIDLLITGKEADPTIIQALRNKGLDIILA"
     gene            2056..2916
                     /locus_tag="PXB27_002020"
     CDS             2056..2916
                     /locus_tag="PXB27_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HDL5346773.1"
                     /translation="MRKHKLGIYEKALPKNISWQDRLSIAKVCGFDFVEMSIDETDER
                     LARLDWSKKERAKLVKTIINTGVTIPSMCLSGHRRFPFGSCDEATRQKAYEIMEKAIQ
                     LAVDLGIRTIQLAGYDVYYEEQDEGTIQRFQQGLEWAVELAASNQVTIAVEIMDTQFM
                     SSISRWKKWDEIIKSPWFTVYPDLGNLSAWNDNVAEELKLGIDKISAIHLKDTYKVTE
                     TCKGQFRDVPFGDGCVDFAACFKTLAELNYRGAFLIEMWTEKAEEPITEIINARRWIE
                     QKMQEGGFTL"
     gene            2913..3632
                     /locus_tag="PXB27_002021"
     CDS             2913..3632
                     /locus_tag="PXB27_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDL5346774.1"
                     /translation="MTQLTELTPALQALRERVLKANLELPKYGLVTFTWGNVSEIDRE
                     TGLVAIKPSGVEYDAMTVEDIVLVDLQGNRVYGDKKPSSDTETHLELYRQFTDIGGVV
                     HTHSRNAVGWAQAGRDLLALGTTQGDYFYGAIPCTRKMTAEEIAGSYELETGKVIVET
                     FRTRGIEPKAVPGVLVHSHGPFTWGKDGHDAVHNSVVLEEVAYMNFISHQIRPQIGPM
                     QQELLDKHYLRKHGANAYYGQ"
     gene            3880..6330
                     /locus_tag="PXB27_002022"
     CDS             3880..6330
                     /locus_tag="PXB27_002022"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012311.1,HMM:TIGR02601.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDL5346775.1"
                     /translation="MKNRNYFVAIATILALSPEVNATELSDQLASRTRYYPSATDSYY
                     SYYKYAPIYSGYYTSNSRNGYSSWYYPGSNAGVTTRTVSTTVNGITVTKVYTTHNGRT
                     TVRTTYAPAVNKPIVNKPTPIANKPVVDTPTQPVIDANPIMDPPVQPIVDNKPITDTQ
                     PNPITDNKPVIESPIDDTRPVIIRPTTPVNLHPEPVAVGVPNVGIRWNDRSRGYNIDD
                     PNNKQNVKLTGEGVKVGILDSGFKNYLMQDDILKKFGNRAVIIETGRTAPASATHGIE
                     VAEMVGGGNSRNGVASKSTLLLADITKTTNAGTGLSATAPIYYDLWNRGARIFNQSYG
                     IPKPLTYFNNNRNSSYYYLHQFDGNVLQFYKDKVKEGGLFVWAAGNNRGDLDPSLQAG
                     LPHYEADLEKGWISVVAVATRTNQGLGNFEWSNLLPYSQAGVAKNWTVTAMGDYIFNL
                     GGRNIIASGSSFAAPAVTGTAALVKQKYPWMDGNLIKQSILSTATDIGAPGVDDVYGW
                     GLLNIEKATNGPARFGKRLALADNVTVNIPNGSYEFSNNINGDAGLVKNGQGELILSG
                     ASTFEGDTTLNEGAVTINGKKYVSKVNVSPSGTLVVNNTTLENGVNNEGTVVNQGYST
                     IKRAYVASAQSTLVSDLGSNLNVKGEVDLNGSTLSLAAVKEGKAQYVTQKGLSMSAIT
                     SDSAIKGNFAAIETSGLLTATAEKTNENEVKATVSRKNVATALESSFSALDNQIEGNN
                     GSTNATNDEFAQQAATLQNSIANINTQSVVLDSLSGQIYASAQALTFENSETVNKDLS
                     NRLVMLGTLDNVGETAGV"
     gene            6340..>7115
                     /locus_tag="PXB27_002023"
     CDS             6340..>7115
                     /locus_tag="PXB27_002023"
                     /inference="COORDINATES: protein motif:HMM:NF015737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDL5346776.1"
                     /translation="MYGNGTLKEIGFGEGKTNTYAGQIGFDKPVTDHVILGAALNYSK
                     AKVNFDRYGGSSKADGIGASLYARIGNKHKTPWYLQGRAGYGSVDSDVERHIILADNN
                     ASTAKINHRDRVFSAYVESGYDFKQGRFALTPFVGFSHDVVRRGAFSEQNSQFGLTAD
                     KATYKQTSGLIGLRTSLEVNLAGMKTTFQGYVNHQKAFNREDLSFKASYTGLQDAKFD
                     VKGIGLAKHKTWVGIGALAEVNKNTSLYVNYDLKLAKNSG"
BASE COUNT         2219 a         1328 c         1612 g         1956 t
ORIGIN      
        1 agatgtacaa atttttgtga ttgtttatga ttggttttga ttgcattgtt cttattttag
       61 cgtaaatcag gagactatta tgagcaaaat tcaagaaatc actcgtgaaa gctggatcct
      121 ttcaactttc cctgaatggg gtacttggtt gaatgaagaa attgaagaag aagttgtacc
      181 ggaaggcaat tttgcaatgt ggtggctagg ctgtgtcggt gtgtggatta aaaccccagc
      241 tggtgcgaat atctgtatgg atttatggtg cggtcgtggt aaatccacca agaaagtaaa
      301 agatatggtg cgtggtcatc aaatggcaaa tatggcaggg gtgcgtaagc tccagcctaa
      361 tttgcgggct caaccgatgg tgttagatcc gtttgcgatt aacgaagtgg acttcattct
      421 cgcttctcac tatcactcag accatattga tgtaaacgtg gcagcggcta tttgcaataa
      481 cccgaaatta gaccacgtta aatttgtcgg cccttggcac tgtgcagaat tatggaaaaa
      541 atggggcgtg ccggaagaac gtattattgt ggtaaaaccg ggcgatgtcg tgaaattaaa
      601 agatgtagaa attcacgccc tagattcatt tgaccgtacc tgtttagtga ccttaccggt
      661 agaaggtgca gaagaacgtg gcggagaact tgcaggctta tgcccatcag atgaagaaat
      721 gggacgcaaa gcggtgaact atgtgttcaa aaccccgggc ggtacgattt atcacggtgc
      781 agattctcac tactcaatcc aattcgctaa acacggtaag caatttgata ttgacgtagc
      841 gctgaataac tatggcgaaa acccggttgg tattgcggat aaaatgactt cagttgattt
      901 attacgtatg gcggagtgtt tacgctgtaa agtgattatt ccggttcacc atgatatttg
      961 gacaaacttt atggcaagta cgcaagaaat tattgattta tggcgtatgc gtaaagatcg
     1021 cttacaatac caattccacc catttatttg ggaagtgggc ggcaaatatg tttacccacg
     1081 tgataaagac ttaattgaat atcatcaccc tcgcggcttt gatgactgct tcgagcaaga
     1141 gccaaatatt caatttaaat ccatgctata ggtgagcagt cgccactctc actggagagt
     1201 ggcgttttag caagttcatt atggagcagt agtgattagt aatgggataa agtagtgaac
     1261 gaaagttata gacacaaaga gttattagcc ctgttggaag agcggaaagc actgtcaaca
     1321 gcagatatta tgcagctgtt aaatatttct ccggcaacag caagacgtga tatcagtaaa
     1381 ttagatggtc aaggtaaatt aaaaaaggtt agaaacggag cagaagcaat tctggctcct
     1441 acatttttac agcacacaaa aataactaac aatgcgcagg agaaacgtag aatagcggaa
     1501 gctgccggtc gtttatgtaa agatggggaa agtcttattc tgacgtgtgg ttccacaatg
     1561 gaaatgctcg gtgaagtatt atgtggtaga aaattgcaaa ttattaccaa ttatctaccg
     1621 cttgcaaacc agttgattgc aggttgccac gatgatgtag tgattatggg tgggcaatat
     1681 aataaacata aaaaagtgat gctctcaatt aacacccaaa atgagatgac ttatgccgct
     1741 accacaatgt ttaccagtgg caaaggctta accagcgaag gattatttaa aaccgatatg
     1801 attattgcca atgccgagca acaaatggca aagcacgcag aacgttatgt ggtgttattg
     1861 gatagcacca agctcggcaa aaaagtcggt atgctgttta aagagttcga taaaattgat
     1921 ttattgatta ccggcaaaga ggcggatccg actattatcc aagcattaag aaacaaaggc
     1981 ctggatatta tcttggctta gcctgtagcg gtcaaattta atcaaaattt tacttatcat
     2041 cttcaaggga aaactatgag aaaacataaa ctgggtattt acgaaaaggc cttgccgaaa
     2101 aacatttcgt ggcaagaccg cctttctatc gccaaagtct gcggttttga ctttgtggaa
     2161 atgtcgattg acgaaacgga cgaacgctta gcccgccttg attggagcaa aaaagaacgt
     2221 gccaagctgg tgaaaaccat catcaacacc ggcgtgacca ttccgtcaat gtgcttgtcc
     2281 ggtcatcgcc gtttcccgtt tggttcttgt gatgaagcga cccgccaaaa agcctatgaa
     2341 attatggaaa aagccattca actggcggtc gatctcggta ttcgcaccat tcagcttgcc
     2401 ggctatgacg tctattacga agagcaggac gaaggcacaa tccaacgctt ccaacaaggc
     2461 ttagaatggg cggtggagct ggcggcaagc aaccaagtta ccattgcggt ggaaattatg
     2521 gacacccaat ttatgagttc gatttcccgc tggaaaaaat gggacgagat catcaaatca
     2581 ccgtggttca ccgtgtatcc agatttaggc aacctctcag cgtggaacga caacgtagcg
     2641 gaagagctga aactcggcat cgacaaaatc tctgccattc acttaaaaga cacttacaaa
     2701 gtgactgaaa cctgcaaagg gcagttccgt gatgtgccat ttggtgacgg ttgcgtagat
     2761 ttcgccgctt gttttaaaac ccttgccgag ctgaattacc gtggcgcatt cttaattgaa
     2821 atgtggacgg aaaaagccga agagccgatt actgaaatca tcaacgcccg ccgttggatc
     2881 gaacaaaaaa tgcaagaagg aggatttacc ctatgacgca attaactgaa ttaacccctg
     2941 cattacaagc cttaagagag cgtgtgttaa aagccaattt agaattaccg aagtatggct
     3001 tggtaacttt cacttggggt aacgtaagtg aaatcgaccg tgagaccggt ttagtcgcca
     3061 tcaaaccttc cggcgtagaa tacgatgcga tgacggtgga agacattgta ttggttgatt
     3121 tacaaggtaa tcgagtttac ggagataaaa aaccgtcttc tgatacagaa acgcacttag
     3181 aactttatcg ccaattcact gatattggcg gtgtggtaca cactcactct cgcaatgctg
     3241 taggttgggc acaagcaggg cgtgatttat tggcacttgg caccactcaa ggcgattatt
     3301 tctacggtgc gattccttgt actcgcaaaa tgaccgcaga ggaaattgcc ggcagttatg
     3361 agctggaaac cggaaaggtg attgtcgaaa ctttccgaac tcgaggtatt gaaccgaaag
     3421 cagttccagg tgtgttggta cattcacacg gtccatttac ctggggaaaa gatggacacg
     3481 atgctgtgca taattctgtg gtattagaag aggtggctta catgaatttt attagccatc
     3541 aaattcgccc acaaattggc ccaatgcaac aggaattatt agataagcat tatctgcgta
     3601 agcatggtgc aaatgcttat tatgggcaat agctaataac ctaaaaatta ttttccaaat
     3661 tatcaacagc tttcgagggg ctttctataa tcggaatagc tcctcagtct gtttgagttt
     3721 atggatagat ccacaaataa ttaccatttt ccttttcttg attttaattt tgatttttgt
     3781 tctaatctct gcctcttgtt tttacaagtc tattaaggat ccgtagtaga tttgaaataa
     3841 acaagagtat ctttgtattt attagaagga aaccaaaagg tgaaaaatag aaattatttt
     3901 gttgcaatag caacaatatt agccttatcg ccagaggtta acgcaactga actttcagat
     3961 cagcttgcaa gtagaacaag atattatcct tccgccacgg atagttatta tagttactat
     4021 aaatatgcac cgatttattc gggctactat acatcgaata gccgtaatgg ttattcgtcg
     4081 tggtattatc caggtagcaa tgcaggtgta acaaccagaa ctgtttcgac aacagttaat
     4141 ggcattacag ttacaaaagt ttatacaacg cataatggga gaacaaccgt tcgtaccact
     4201 tatgctcctg ctgtgaacaa accaattgta aataaaccga caccgattgc caataaaccc
     4261 gttgtagata cgccaactca accggttatt gatgccaacc caattatgga tccgccagtc
     4321 caaccaatag ttgataataa acccatcact gatacacagc ctaatccaat tacagataac
     4381 aaacctgtca ttgaatcgcc aattgatgac acccgtccag ttatcattag gcctacaaca
     4441 ccggtaaatc ttcatcctga acctgtagct gttggtgtac ccaatgtagg tattcgatgg
     4501 aatgaccgct ctcgtggtta caacattgat gatcctaata acaaacaaaa tgttaagtta
     4561 actggtgaag gtgttaaagt cggtatctta gatagtggtt ttaaaaacta tttaatgcag
     4621 gacgatatac tcaaaaaatt tggtaatcgc gctgttatta ttgaaacagg gcgtactgcc
     4681 ccggcttctg caacacatgg tattgaggtc gcagaaatgg ttgggggagg gaactctcgc
     4741 aatggagtag cgtctaagtc aacgttatta cttgcggata ttaccaaaac aacaaatgcg
     4801 ggtacaggat tatccgcaac agctccaatt tattatgact tatggaatag gggagcccgt
     4861 atttttaacc aatcatacgg tattccaaag cctcttactt attttaataa caatagaaat
     4921 tctagctatt actatttaca ccaatttgat ggtaatgtgt tgcaattcta taaggataaa
     4981 gtaaaagagg ggggcttatt tgtctgggct gcggggaata accgtggtga tcttgatcca
     5041 tcactccaag ctgggttacc tcattatgaa gctgacttgg aaaaaggttg gatttctgtt
     5101 gttgcggtag caaccagaac taatcaggga ttgggtaact ttgaatggag caatttattg
     5161 ccatattctc aagccggtgt ggcaaaaaat tggaccgtta ccgcaatggg tgactacatc
     5221 tttaatttag gaggtagaaa cattattgct agtggttcat cctttgcagc tccagccgtt
     5281 acgggtacag cagctctcgt gaaacaaaaa tatccttgga tggatggtaa ccttatcaaa
     5341 caatctattc tctcgacggc aaccgatatc ggcgctcctg gagttgatga tgtatatgga
     5401 tggggtttac ttaatattga aaaagccact aatggccctg cgcgttttgg taaacgactc
     5461 gctttagccg ataacgtgac tgtaaatatt ccaaatggaa gctatgagtt ttccaataat
     5521 attaacggtg atgcaggctt ggtgaaaaat ggccaaggcg aattaatttt atcgggtgct
     5581 agtacttttg aaggggatac aacactcaat gaaggtgctg tcactattaa cggtaaaaaa
     5641 tatgtgtcta aagtaaacgt atctccaagt ggtacattgg ttgtcaataa taccacgtta
     5701 gaaaatggcg taaataacga gggtacagtt gtcaaccagg gttattctac gattaagaga
     5761 gcttacgttg cttcagcaca aagtacgcta gtgtcagatt taggttcaaa cttaaatgta
     5821 aaaggagaag ttgacttaaa cggctctact ttatcactag ctgcggtaaa agagggtaaa
     5881 gctcagtatg tgacccaaaa aggtttatca atgagtgcta ttacttctga ttccgcaatt
     5941 aagggtaact ttgctgcaat agaaacctca gggttattaa ctgcgacggc ggaaaaaacc
     6001 aatgagaatg aagtaaaagc aacagttagc cggaaaaatg tcgcgactgc tttagagagc
     6061 tcattttcgg cattggataa ccaaattgaa ggaaataatg gttctacaaa tgctacaaat
     6121 gatgaattcg cacaacaagc ggctacttta caaaacagta ttgcaaacat caatacgcaa
     6181 agtgtggttt tagatagctt atctggtcaa atttatgcgt ctgcgcaagc gcttactttt
     6241 gaaaactcag aaacagtgaa taaagatctc tctaaccgtc ttgtaatgtt aggaacgctg
     6301 gataatgtag gtgagactgc tggtgtgtga gtaacgggta tttatgggaa tggtactcta
     6361 aaagaaatag ggtttggtga aggtaaaacc aatacctatg ctggccaaat aggttttgat
     6421 aagccagtta cagaccatgt gattttaggt gcggcactta attattctaa agcaaaagtg
     6481 aacttcgatc gttatggcgg tagttcaaaa gcagatggaa taggtgcatc attatatgcc
     6541 cgtataggca ataagcataa aacaccttgg tatttacaag gtcgagccgg ttatggcagt
     6601 gttgattcag atgtagagcg tcatattatt ttggcggata ataacgcaag tacagcaaaa
     6661 atcaaccacc gagacagagt attttctgct tatgttgaat caggctatga cttcaagcaa
     6721 ggtcgctttg cgttaactcc atttgtcggt tttagtcatg atgttgtcag acgtggcgca
     6781 ttcagtgagc aaaacagtca atttggtctc accgcggaca aagcaaccta taaacaaaca
     6841 agcggattga ttggtttacg aacatcttta gaggtgaatt tagctgggat gaaaacgact
     6901 ttccaaggct atgtaaatca tcaaaaagcg tttaatcgag aagatcttag ctttaaagca
     6961 agttatacag gtttacaaga tgcaaaattc gacgtaaaag gtattgggct tgcaaaacat
     7021 aaaacgtggg tagggattgg tgcattggcg gaagtaaata aaaatacttc cttatatgtg
     7081 aattacgacc ttaaactcgc aaaaaatagt ggtca
//
LOCUS       DANLUQ010000130         7115 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.131, whole genome shotgun sequence.
ACCESSION   DANLUQ010000130 DANLUQ010000000
VERSION     DANLUQ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7115
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.131"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            31..2330
                     /locus_tag="PXB27_002024"
                     /pseudo
     CDS             31..2330
                     /locus_tag="PXB27_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005759321.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(2408..3109)
                     /locus_tag="PXB27_002025"
     CDS             complement(2408..3109)
                     /locus_tag="PXB27_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HDL5346777.1"
                     /translation="MDLQNRENFLNKLAQRMGRPRQNVPEPIPVLENNHAITRLTDRP
                     QPQLCQEFIDFARVMMADVVEAKEADLPQAIIDICEKYGGASVIVNNDQRLKDLGITA
                     AIQAKYEDSYVWDFNQAETNFEKARTANIGVVYGEYGLAESGGIVLFSDKEFGRSVSL
                     LPEKSVVVLRKSTVLPRVAQLAQILHEKAQRGERMPSCVNIISGPSATADIELIKVVG
                     VHGPVNKIYLVIDDL"
     gene            complement(3114..4523)
                     /locus_tag="PXB27_002026"
     CDS             complement(3114..4523)
                     /locus_tag="PXB27_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HDL5346778.1"
                     /translation="MSYLQTNTLPFKQRVEQQVNNEIVRKALVKAQETIGANRQRMVD
                     ELGNWEEWRDDAKAIRNHVLANLDAYLYQLSEKVTQNGGHVFFAETAEEATDYIKNVA
                     KAKNAKKIVKSKSMVTEEIGMNHVLEAEGIKVVETDLGEYLLQIVGDKPSHIVVPAIH
                     KDRHRIRQEMHDVLGYQGSETPEEMTAFVRQKIREDFLEADIGISGCNFAVPETGSVC
                     LVTNEGNLRMATTVPKTHIAVMGMERIAPTFKEVDVLITMLARSAVGAKLTAYNTWLT
                     GPRLEGETDGPEEFHLVIVDNGRSKILESEFQEVLRCIRCGACLNTCPAYRQIGGHGY
                     GSIYPGPIGSVISPLLGGYEEFKELPYACSLCTACNSVCPVKIPLAQLILKHREKIAD
                     AGLTPMAERLSIFGFNFANSHPTLWKVGVKVGAKVASKLIKNGKAPVNFGALGEWTKA
                     RDLPTAEGESFREWFNNRG"
     gene            complement(4525..5256)
                     /locus_tag="PXB27_002027"
     CDS             complement(4525..5256)
                     /locus_tag="PXB27_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HDL5346779.1"
                     /translation="MNVNFYVTCIADVMKAGVAKNTVLLLEKLGCKITFLEKQGCCGQ
                     PAINSGYTKQALAGMKNLVETFEANDYPIVAPAGSCVYAIKTYPDHFERFGEKEWAER
                     AKKVADRFYDLTDFIVNKLGVKDVGAYLPGKAVYHPSCSLFRKLGIKDEPIALLQHVK
                     GLELLPIKNQETCCGFGGTFSVKMAEISGEMVKEKVANVDTDEPDYLIGADVSCLMNI
                     GGRLSREGKPIKVMHIAEVLMQGGK"
     gene            complement(5400..7004)
                     /locus_tag="PXB27_002028"
     CDS             complement(5400..7004)
                     /locus_tag="PXB27_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005818537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HDL5346780.1"
                     /translation="MALFLSVFPIVLLIYLMVKRNALPSYVALPLTALLIFVLQLTYF
                     GNDTTLIFANIIAGLGDVLTPITVIFGAILFNRFSEVSGATNTMRKWLGTINPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILVGLGFKPLQVAMLALVMNSVPVSFGAVGT
                     PTWFGMGPLLKDGLLTDAQVLEIGSITALIHSIAAFIIPVMALRLIVSWKEIRQNIVF
                     IYISIFACVIPYFIIAQFNYEFPSLVAGAIGLLVSVWVANMGIGLAKSENHLDGDKAT
                     FGEVAKALLPTGLLIFILVITRIQQLPLKAMLNDATAWIVSSLGFANFEISQGLIFAL
                     KNILGTNVATSYKLLYVPALIPFVVTVLICLPIFSVSGKNTQEIFGTSFKQVKKPFLA
                     LLGAIVMVKLMLIGGVDSMVQIIGKNFAEFTGQSWTLFASFLGAIGAFFSGSNTVSNL
                     TFGTVQYATAQQVGLSVTLILALQSVGGAMGNMVCINNIVAVCSVTGIEKQEGNIIKK
                     TAVPMFVYGVIAAISALFIVPMFYNL"
BASE COUNT         2184 a         1555 c         1344 g         2032 t
ORIGIN      
        1 atggctattt attctagaag gactctaaac gtgttaaaga aaaattatct cactgtatca
       61 attctccttg ccattagtgg agtaggctac gctaatgaaa tctctttaga gactattact
      121 gttgatggaa atacgccttc aaccaaaggg aaattactag gtggtgaact aaatagcaat
      181 gaatctgttg ttgatgaaaa aaattaaaac aaggtagtat tacattaggt aacgctttat
      241 ctggtgaatt aggcattcat tcaagccagt tcggaggcgg tgcaagtacc ccaattattc
      301 gtgggcaaga gagtaaacgt gcaaaaatct tacaaaataa tggcgaaaac ttagatatgt
      361 cgggaatgtc tcctgatcat gcagtaaccg tagatgcgct acttgctaaa cgtattgaaa
      421 ttttacgtgg tccaactact ttactttaca gtgcaggtaa tacggcaggt gtaattaatg
      481 tggtagacaa taaaattcca accgctattc ccgaaaaagg ctatgagggg caatttggtg
      541 tgcgttttgg ctcagccagt aaagagcgtt taacctatgc aggctcaacc tttgcccttg
      601 gaaatcattt agccttgcgg gtacagggaa tgtataacaa agcttccgaa tactatgctc
      661 cacactttac gattgaaggc aaaccttacc accgcgtacc agatagcgat gtacaatcac
      721 aaaccggcac agtaagcctt tcttggattg gagagcgagg acatcttggt attgcttaca
      781 ctgatcgccg tgataaatat ggattaattg gtcacacaca caaatacgat cactatacta
      841 ttagtattat tcgccaagcc gttatgtttg caaaaggtta tttacgtttt tatccgcacc
      901 ttgcagaaga aggagatatt gactataaca accctggtat ccgtttactg catactcata
      961 ttccgggagg ctcacattat ggacaagata ctcacgagca cggcaaacct tggattgata
     1021 tgcactccaa gcggtatgat attgatggaa gtttgcaaaa tccacttcct ggttttgaag
     1081 aagccaaaat atcagctaac tatgtagatt actatcatga tgaaaaagat ggaaaaagag
     1141 ttgaaaatta cttcaagaac aaaggaaaaa atctacgttt cgaattggtt cacaaagagt
     1201 ggaaagggct aaaaggagct attggtgtgc aatataccaa ccaatctacc tcagcacttg
     1261 cacttgaagc ttcacgagct gcaaaagtat ttaataaaca gcctttactc aataatccaa
     1321 aaacaaaatt atggagtctc tttgcgattg aacgccttaa tttaggtgac tttacctttg
     1381 agttaagtgg gcgggcagaa cgtcaaaaaa tagcaatgga ttatgatgtg aaattaattg
     1441 atcgctggct aggctttaat acacctatgc caaacttgga cccacataaa gacaaaggtt
     1501 attcttatag ctttgcaact cattggtatt tcgcaccaaa ccataagtta acattaaatg
     1561 ctgcacatca agaacgttta cctaatgctc aggaactcta tgctcatggt aaacacattg
     1621 ctttaaatgc atttgaggct ggcaataaaa atctaaaaaa agagcgttct aaccaaattg
     1681 agctatcatt agcttatgtt ggcgacaaat gggattataa attaaatctc taccacaccc
     1741 gctatggcaa ctatatttac ccattaactc ttaatgataa tcgtggacct aaatctttta
     1801 ccgatgaata taacttgaaa gtgaatagat attatcaagg cgaagctcgt tttagtgggg
     1861 cagaaggcga aattggttat ctatttacac caaattatcg cttagccgta ttcggagact
     1921 atgtccgtgg aaagttagtc aacttaccta atattgcaat gagttacaac atttggacag
     1981 gtgaagttga caaatgggct tctcagccag acatttctgc acctcgtata ccaccactac
     2041 gtttaggagc aagatttaat gctgacttca acctaaactg gtctggaatg ttagaatatt
     2101 accgagtatt tgctcagaaa aaagtctcga aatacgaaca agttacccct ggccatcatc
     2161 aagtaaacct tggcgtaacc tatagtaatc attttaatca aacagaatac caagtattcc
     2221 ttaaagtgga taacttgctt aatcagaaaa tgtaccaaca tgcctcatat ctaccacata
     2281 ttccacaaat gggtagaaat gcaatgcttg gaatgaatat cagtttttaa tttgtaaatc
     2341 tctacaaaaa tataaccgct tgtaaatttc tatctacaag cggttacttt cagtggattt
     2401 tttgcaatta gagatcgtca attaccaagt aaattttatt taccgggccg tgaacgccaa
     2461 ctactttaat caactcaata tccgcagtcg ccgatgggcc tgagatgata ttcacacaag
     2521 atggcattct ttcacctcgt tgggcttttt cgtgcagaat ttgtgcaagc tgtgccacac
     2581 gaggtaacac tgtacttttg cgtaacacaa ccaccgattt ttcgggcaat aagctcactg
     2641 aacgcccaaa ttctttatcg gaaaacagta caataccgcc agactctgcc aagccatatt
     2701 caccgtaaac cacaccgata ttagcagttc tggctttttc aaagttggtt tcagcttggt
     2761 tgaaatccca cacatagctg tcttcgtatt ttgcttgaat ggctgcggta atcccaagat
     2821 cctttaaacg ttgatcgtta ttcacgatca cactcgcccc gccatatttt tcacaaatat
     2881 caataatggc ttgtggtaaa tccgcctctt ttgcctctac tacatctgcc atcatcactc
     2941 gggcaaaatc gataaactct tggcaaagtt gtggttgagg gcgatcggtt aaacgggtaa
     3001 tcgcgtggtt gttttccaac acaggaattg gctcaggcac attttggcgt ggtctgccca
     3061 tacgctgtgc caatttattt aagaaatttt cacgattttg taaatccatt ttcttatcct
     3121 ctgttattaa accattcacg gaaactttcg ccttcggcag tcggtaaatc acgggctttc
     3181 gtccattcac ctaacgcccc aaaattcacc ggtgctttgc cgtttttaat caatttgctt
     3241 gccactttcg cccccacttt tacgccgact ttccaaaggg ttgggtgtga gttagcgaag
     3301 ttaaagccaa aaatagacaa gcgttctgcc attggcgtta agccggcatc cgcaattttc
     3361 tcacggtgtt ttaagataag ctgtgccagt ggaattttca ctggacaaac actgttacaa
     3421 gcggtacaaa gtgagcaagc atacggcaat tctttaaact cttcgtagcc accgagcaat
     3481 ggcgaaataa ctgaaccaat cgggcccgga tagattgaac catagccgtg accgccaatt
     3541 tggcgatacg ccgggcaagt gttcaaacat gcaccacagc ggatacaacg caacacttct
     3601 tggaattcgc tttctaagat tttagaacga ccgttatcga caatgaccaa gtggaactct
     3661 tccggaccat cggtttcgcc ttcaagtcta ggacctgtca gccaagtgtt gtaagcggtg
     3721 agttttgccc ctaccgcact gcgagccagc atggtaatta atacatcaac ctctttaaag
     3781 gttggggcga tacgttccat acccattact gcaatatgcg ttttcggcac agttgttgcc
     3841 atacgcagat taccttcgtt ggtcactaaa caaaccgagc cggtttccgg caccgcaaag
     3901 ttacagcctg aaataccgat gtctgcctct aagaaatctt cacggatttt ttggcggacg
     3961 aatgcagtca tctcttcagg tgtttccgaa ccttgataac ctagcacatc gtgcatttct
     4021 tggcggattc tgtggcgatc tttgtggatt gcaggtacaa caatatgaga aggtttatcg
     4081 cccacaattt gcaataaata ttcgcccaaa tcggtttcaa ccactttaat gccttctgct
     4141 tctaaaacgt ggttcatccc aatctcttcg gttaccatcg acttcgattt tacgattttc
     4201 ttcgcatttt tagctttcgc cacgtttttg atgtaatcag tggcttcttc ggcggtttcg
     4261 gcaaagaaaa cgtgtccgcc gttttgggtg actttttcgc ttaattggta gagataagcg
     4321 tctaaattgg ctaaaacgtg gttacgaatt gctttcgcat catcacgcca ctcttcccag
     4381 ttgcccagct catccaccat acgctgacgg tttgcaccga ttgtttcctg agccttaact
     4441 aaggctttac gcacaatctc gttattaact tgctgctcaa cacgttgttt aaacggcagg
     4501 gtgttagttt gtaaataaga catcttattt tcctccctgc attaacactt cggcaatgtg
     4561 catcactttg attggcttac cttcacggct taaacgaccg ccaatgttca tcaagcagct
     4621 tacatctgcc ccgattaaat aatccggctc atcggtatct acatttgcga ctttctcttt
     4681 taccatctcg ccggaaattt ctgccatttt gaccgagaat gtgccaccaa aaccacagca
     4741 agtttcttgg tttttgattg gtaataactc taacccttta acgtgctgta ataaagcaat
     4801 aggctcatct ttaatcccca gcttacggaa taagctgcaa gacgggtgat aaactgcttt
     4861 gcccggtaaa taagccccaa catctttcac gcccagtttg ttcacaataa aatcggttaa
     4921 atcatagaaa cgatcagcca ctttcttcgc acgttctgcc cattcttttt cgccaaagcg
     4981 ttcaaaatga tccggatagg ttttgatcgc atatacacaa gaacctgccg gggcgacaat
     5041 cgggtaatca ttagcttcaa aggtttcaac caagtttttc atacctgcta aagcttgctt
     5101 agtgtaaccg ctgttgatcg caggttgtcc acagcaacct tgtttttcta agaaagtgat
     5161 tttacaacct aatttttcca ataaaagcac agtatttttt gcaacccctg ctttcattac
     5221 atctgcaatg caggttacgt aaaaattaac gttcataaag aactcccaca gtaaatataa
     5281 ataagaatta aactcaaaaa atgccgttct aagacggcga aaggaagagg tggatttgca
     5341 aaaaatttgc taaatctcac cgcttccttt ctaaatcaag taagctgatt attaactcat
     5401 tataggttat agaacatagg tactataaac aatgccgaaa tcgcagcaat aacgccatac
     5461 acgaacattg gcacggctgt tttcttaata atattgcctt cttgtttctc aatgccggta
     5521 acagaacaca cagccacgat gttgttaata cacaccatat tccccattgc accaccaact
     5581 gattgcaatg caagaattaa ggtaacagat aacccgactt gctgtgccgt tgcatattgt
     5641 actgttccga aggttaagtt agataccgta tttgaccctg agaagaacgc accaattgca
     5701 cctaagaacg aggcaaataa tgtccaagat tgaccggtaa attctgcgaa gtttttacca
     5761 atgatctgaa ccattgagtc tacaccacca attaacatta attttaccat cacaatggca
     5821 cctaaaagtg ctaagaatgg ttttttcact tgtttaaatg aagtgccaaa aatctcttga
     5881 gtatttttac ctgataccga gaaaatcggt aagcaaatta atacggtcac aacaaatgga
     5941 atcaacgcag gaacatacag taatttatag ctagttgcta cattcgtacc taaaatattt
     6001 ttcagtgcaa aaattaagcc ttggctaatt tcaaagttag cgaaaccaag cgaactgaca
     6061 atccaagctg ttgcatcatt tagcattgct tttaatggaa gttgctgaat acgggtgatt
     6121 accaaaatga agatcagtaa ccctgttggt aataatgctt ttgccacttc accaaaggtc
     6181 gctttatcgc cgtcaaggtg gttctcgctt ttcgctaaac caatacccat atttgctacc
     6241 cagactgaca ccaataaacc aatcgccccg gcaactaatg atgggaattc gtagttaaat
     6301 tgagcaataa tgaagtaagg aatgacacag gcaaaaatgc tgatataaat aaacacgata
     6361 ttttgacgaa tttctttcca gctcacaatt aaacgcaatg ccataactgg aataataaac
     6421 gcagcaatag agtggatcag tgcagtaata gaaccaattt caagcacttg tgcatcggtt
     6481 agtaaaccat cttttaataa tggtcccata ccaaaccaag ttggtgtgcc aaccgcacca
     6541 aatgacaccg gcacagagtt cattactaag gctaacattg ccacttgtaa cggtttaaag
     6601 cctaaaccaa ccagaattgg agctgcaatt gccgccggcg taccgaaacc tgatgcgcct
     6661 tcaatcataa atgcaaatgc ccaaccgata atcatcaact gtgccaccgg attcgggtta
     6721 attgtcccta accatttacg catagtgttg gttgcacctg aaacttcaga gaaacggtta
     6781 aataaaatcg ccccgaaaat aacggtaatt ggggttaata catcacctaa accggcaata
     6841 atattggcaa agattaacgt ggtatcatta ccaaaatagg taagctgaag cacaaaaatt
     6901 aataacgccg taagtggtaa agccacataa gacggtaacg cgttacgttt taccattaaa
     6961 taaatgagca aaacaattgg aaatacgctc aggaatagag ccatactgca cctctttata
     7021 tgattataaa atgacctaac tggtcggata ggtgcgaatt gtactcttaa aaaaaactaa
     7081 tgtaactagg ttgttacaat tttgtgatct agctc
//
LOCUS       DANLUQ010000131         7089 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.132, whole genome shotgun sequence.
ACCESSION   DANLUQ010000131 DANLUQ010000000
VERSION     DANLUQ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 7089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7089
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.132"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..593)
                     /locus_tag="PXB27_002029"
     CDS             complement(<1..593)
                     /locus_tag="PXB27_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5346781.1"
                     /translation="MAEILFRSCLCCNAKRIFYLLTFYKGFETSQIATAFSLLVFTSQ
                     AFSLFAGSWGDSFGRYPIMMLGCLLDALAYILLLTTKNYPLLLLATFFFGLGSTLFST
                     NARAFLLANIQDTGDSYQLKTKAQGKFLKVSSLSAMVAPLLTIPFIFYRKAEWLIWIS
                     CCIEIIMLFVMFKTAPKTHECANGQCERFKFGALKGIL"
     gene            676..1137
                     /locus_tag="PXB27_002030"
     CDS             676..1137
                     /locus_tag="PXB27_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HDL5346782.1"
                     /translation="MRKIKLLHVILSLILGLSAAAIGSYPAIKEIGEERAKKHQDILD
                     LNRELYSAMVNRDFSALQTLLSDDFELYSSTGEVIDKQEWIKLIQKRVLEFDKVDLLS
                     TDFQGNQSSGLLRVNGEFFGIERDNVVVEMNIRTIVRNTERQIKCIVIKEV"
     gene            1150..2043
                     /gene="murQ"
                     /locus_tag="PXB27_002031"
     CDS             1150..2043
                     /gene="murQ"
                     /locus_tag="PXB27_002031"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDL5346783.1"
                     /translation="MNALGQMITEQRNPNSMNIDRLSALDIVQVINQEDKQVAIAVEQ
                     CLPQIAQAVEKIVQAFEKGGRLVYVGAGTSGRLGVLDASECPPTYGVKPEMVVGLIAG
                     GERALRFPIEGAEDNREQGKVDLQAVNFSEKDVLVGIAASGRTPYVLGAMEYAKQLGA
                     TTVSIASNPNSAMAQIADIAIETVVGPEVLTGSSRMKSGTAQKLVLNMLTTASMILIG
                     KCYQNLMVDVQATNEKLKARAIRIVMQATECEREVAEDTLKLAENNAKLAIMMILGNL
                     DKQKAEDLLSQNQGRLQKALK"
     gene            complement(2127..3071)
                     /gene="prmB"
                     /locus_tag="PXB27_002032"
     CDS             complement(2127..3071)
                     /gene="prmB"
                     /locus_tag="PXB27_002032"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDL5346784.1"
                     /translation="MFKYNTELLDEISALPIVNDLVTIQDMMRWGYSYFNASDLYYGH
                     GYDNAWDEAQQLVLAGLYLPFDVPETLYSSRLTRVEKERIINLICERLGSRKPVAYLT
                     NSAWFCGGEYYVDERVIVPRSPIGELIKKGFKGIIKNEPHRILDMCTGSGCIAIACAE
                     RFPEAEVDAVDLSLDALDVAQINIERHQVSHRVFPICSDLFNNLPQDKYDLIVTNPPY
                     VDEEDLGDMPEEFHHEPALALGSGKDGLDLTKRILAQAADYLNDDGVLVCEVGNSMVH
                     LIEQFPSVPFNWLEFEHGGLGVFSLTKEELVKHRHLFA"
     gene            3164..3709
                     /gene="smrB"
                     /locus_tag="PXB27_002033"
     CDS             3164..3709
                     /gene="smrB"
                     /locus_tag="PXB27_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HDL5346785.1"
                     /translation="MNDTLLDDEFALFREAAKGTKKLTQNTFVPKAEPRKKLQELREL
                     KEKADTEFYFSDEYEPLLKEENEKVKYLREDVDPYILKQLRRGDFQPELFLDLHGLTR
                     EKAKKELAALILACEREKVYCASVMTGFGTRTLKDQIPRWLVQHPKVMALHQAPKEWG
                     GDASILILVEQPENPEKMFGR"
     gene            3773..4258
                     /locus_tag="PXB27_002034"
     CDS             3773..4258
                     /locus_tag="PXB27_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDL5346786.1"
                     /translation="MSEQTFHQLTALLDSHRARYRVVGHPSAGKSEEVAKIRGTEIGQ
                     GAKALVCKVKGNGVKQAVLAILPADYQADLTKIAEHLGGLRASLASPAEVAELTDCVF
                     GAIPPFSFHPDLLLVAEPSLFERYAELAFNAGTLERSIILNTEDYQKIVNPVLVQFAK
                     S"
     gene            complement(4260..5009)
                     /locus_tag="PXB27_002035"
     CDS             complement(4260..5009)
                     /locus_tag="PXB27_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL5346787.1"
                     /translation="MEKRFEINEQGLSIRCKLFYEKELQQIENVVIVLHGFGSSKDLK
                     SNTKFGERLSSKYKGYAALAFDFPCHGDDARKKLSVEESITYLQLVIDYAKTSLNAKN
                     LYAYGTSFGGYLTLKYLAEKGNPFRKIALRAPAVQIYHSLMNRLSEEERQKLAKGREI
                     LWGFDRQMKISQAFFDELTDITQNDYLDYAEQILILHGTEDEMVALSTSQQFADNNVI
                     ELLPIEGADHAFSNPKLMDIAIGKIVEFFAP"
     gene            5181..6044
                     /locus_tag="PXB27_002036"
     CDS             5181..6044
                     /locus_tag="PXB27_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HDL5346788.1"
                     /translation="MLQLILAILCSVTVGVLIKFARQKGIRIDQSIAVNYIVTTTMTF
                     LLLKPDYQGQSITDIIANNPTSYIFVILSITLPTIFLVQAKALEFAGIIRTDAAQRLS
                     LFLPILAAFTIFGEQVTQNKLIALLLAFVALGCLLWKGNQGMEKGGKIAIISLALVWV
                     GYGVNDILFKQIAKSGSAFPVTLSIAFIGAGCFMFIYLLLKRTQWHVPSVLTGLLLGV
                     LNFGNILFYIQAHQAMKDDPTLVFTGMNLGVICLGTFVGAFFFKEKIHKVNYLGVAIA
                     IVAIICLFYWR"
     gene            6168..6761
                     /locus_tag="PXB27_002037"
     CDS             6168..6761
                     /locus_tag="PXB27_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346789.1"
                     /translation="MKLKALSTLIASSMLYSQSFAVPPEKTAEEPQPFFNKTESGYDL
                     VFNDQNYRLIETELNGEKIQFRAFERIVYVNNPIEPDYQTINLYVPEAYYNNGEINGY
                     QADTAPIFLPNSVGGYMPAKAASYDKKRRDGSPGTILVALSKGYVVASVGARGRTLQK
                     EGKYTGKAPSAIIDSKSAAHKACRFESDHWHHSLCKI"
BASE COUNT         2177 a         1345 c         1547 g         2020 t
ORIGIN      
        1 agtattcctt ttaatgcacc aaatttaaag cgttcgcatt gcccatttgc acattcatgt
       61 gttttgggtg cggttttaaa cataacgaat agcataataa tttcaatgca gcagctaatc
      121 caaataagcc attctgcttt acggtagaaa ataaatggaa tagtaagtaa tggtgctacc
      181 atcgctgaca ggcttgatac cttcagaaat ttaccttgag ccttagtttt gagttggtaa
      241 ctatctccgg tatcttgaat attggctaat aagaatgctc tcgcattggt actaaataag
      301 gtgctaccga gcccgaagaa aaaggtagca agtaatagca gagggtaatt ttttgttgtc
      361 agtaataaaa tgtaagctag ggcatcgagc aaacagccaa gcatcatgat agggtaacgt
      421 ccaaaactat caccccaaga acctgcaaaa agggaaaatg cttggctggt aaagaccaac
      481 aaagaaaatg cagttgcaat ttggctggtt tcaaagcctt tgtagaatgt aagtagataa
      541 aaaatacgct ttgcattgca gcataagcaa gaccggaaaa gaatttccgc cataaaatga
      601 tattttggat attcatagat aatatttaat gagaaaaatg atttctaata taactaaatt
      661 taataaggaa gcacaatgag aaaaatcaaa ttattgcatg ttattttatc tttaatatta
      721 gggctttctg cggcagcgat tggaagttat ccggcaataa aagagattgg cgaggagcgg
      781 gcaaaaaaac atcaagatat attagattta aatagagaac tttatagtgc aatggtgaac
      841 cgtgattttt cggctttgca aacattacta agcgatgatt ttgagcttta ttcatcaaca
      901 ggtgaagtga ttgataagca agaatggatc aaattaatcc aaaagagagt attggaattt
      961 gataaggttg atttgctatc taccgatttt caggggaatc aatcatctgg attattaaga
     1021 gtaaatggtg agttttttgg gatagagcgg gataatgtgg tggtagaaat gaatattcga
     1081 accattgtga gaaatacaga acgccaaatt aaatgtattg tgattaaaga ggtttaagtg
     1141 tcgcaactat tgaatgcctt agggcagatg attaccgaac aacgtaatcc gaattcgatg
     1201 aatattgacc gcctttcagc gttagatata gtccaagtca tcaaccaaga agacaaacaa
     1261 gtagcgattg cggtggagca gtgcctgcct caaattgcac aagcggtgga aaaaattgtg
     1321 caagcctttg agaaaggtgg acgattggtt tatgtcggtg ccggcacgag cggaagatta
     1381 ggggtgcttg atgcttcgga atgtccgccc acttatggcg taaaacctga aatggtggtt
     1441 ggattaattg ccggtggtga gcgggcatta cgtttcccga ttgaaggggc ggaagataat
     1501 cgtgagcaag gcaaagtaga tttacaagcg gtcaatttta gcgaaaaaga tgtattggtc
     1561 gggattgcag ccagtgggcg aacgccttat gtgttagggg cgatggaata tgctaagcaa
     1621 cttggtgcga ccacggtgtc gattgccagt aacccaaatt cggcaatggc acaaattgcg
     1681 gatattgcga ttgaaaccgt tgtcgggccg gaagtgctaa ccggttccag ccgtatgaaa
     1741 tccggcacag ctcaaaaatt ggtattgaat atgctcacta ctgcgtcgat gattttaatc
     1801 ggtaaatgct accaaaattt aatggtagat gtgcaagcaa ctaatgaaaa actcaaagct
     1861 agagcaattc ggattgtgat gcaagcgaca gaatgtgaac gagaagtggc tgaagataca
     1921 ctaaaattag ccgaaaataa cgctaaattg gcaattatga tgattttagg taacttagat
     1981 aagcagaaag ccgaagattt attaagccaa aatcaagggc gattacaaaa agcgttaaaa
     2041 tagaagtatg tctgctctcc ccttgtggga gagagacaga tcaatcattc attcagaatg
     2101 attgatcaga gagaggggag gcagatttaa gcaaataaat ggcgatgttt cactagttct
     2161 tctttggtta aactgaacac ccctaagcca ccgtgttcaa attccagcca gttaaacggc
     2221 acactgggga attgttcgat taaatgcacc atactgttgc cgacttcgca gactagcacg
     2281 ccatcatcat ttaagtaatc agcggcttga gcaagaatcc gcttggttaa atctaaccca
     2341 tctttgcccg agccaagagc taatgccggc tcgtgatgga actcttcggg catatcgcct
     2401 aagtcttctt catcaacata aggcggatta gtcacaatta agtcgtattt atcttgcggt
     2461 aaattgttaa acagatcgga acagatcggg aaaactcggt gagacacctg atgacgctca
     2521 atattaattt gagcaacgtc taaggcatca agggaaagat caaccgcatc gacttctgct
     2581 tccggaaaac gctcggcaca ggcaatcgca atacaaccgc tgccggtaca catatctaaa
     2641 attcgatgtg gctcgttttt gataatgcct ttaaaccctt ttttgatcag ctcaccaatc
     2701 ggtgaacgtg gcacgatcac acgctcatcg acataatatt caccgccaca aaaccaagcc
     2761 gagttggtaa ggtatgctac aggtttgcga gagcctaaac gctcacagat taaattgatg
     2821 atacgttctt tctcaactcg agttaagcga gaggaataaa gtgtttccgg tacatcaaac
     2881 ggcaaataaa gccctgccaa cactaattgt tgtgcctcgt cccacgcatt atcgtagccg
     2941 tgcccgtaat aaagatcaga ggcattaaaa tagctatacc cccaacgcat catatcttga
     3001 atagtgacta aatcattcac aatgggcagg gcggaaattt catcaagaag ttcggtatta
     3061 tatttaaaca ttggcgtgcc ttttttcgta ttgttaaaat ggctgtgtta tactacattt
     3121 tgctttgtgt tagaatgtaa aaatgaataa gaaggaaaag ataatgaacg atacgttatt
     3181 agacgatgaa tttgcattat tcagagaggc agcaaaaggc acgaaaaagc tgacacaaaa
     3241 tacctttgtg ccgaaagcgg aacctcgcaa gaaattgcaa gaattgcgag aattaaaaga
     3301 aaaagctgat acagaatttt atttctcgga tgaatatgag cctttgctga aagaagaaaa
     3361 tgaaaaagtg aaatatctgc gagaagatgt tgatccttat attctcaaac agttacggcg
     3421 aggcgatttc caacccgagc tgtttttaga tctgcatggt ttaactcgag aaaaagcgaa
     3481 gaaagagttg gcagcgttga ttttagcctg tgagcgggaa aaggtatatt gtgccagtgt
     3541 gatgaccggc tttggcactc gcacgctcaa agatcaaatt ccacgctggt tggttcagca
     3601 cccaaaagta atggcgttgc accaggcccc gaaagagtgg ggcggagatg cctctattct
     3661 aattttggtt gagcagcctg aaaatcctga aaaaatgttt gggcgttaaa attcggctga
     3721 attgccaatt ttttattaaa aacgaccgct tgtgtatcat taaggagaaa tgatgtcaga
     3781 acaaactttc caccaactta cagcattatt agattcgcat cgagctcgct atcgagtggt
     3841 aggacatcct tctgccggga aatcggaaga agtggcaaaa attcgaggaa cagaaatcgg
     3901 gcaaggggcg aaagccttag tttgcaaggt aaaaggcaat ggggtaaagc aagctgtgct
     3961 tgctatatta cctgctgatt accaagcgga tttaacaaaa attgcagagc atcttggtgg
     4021 gttaagagcc tcacttgcca gcccggctga agtggcagaa ttgaccgatt gtgtatttgg
     4081 ggcaattccg ccgtttagct ttcaccccga tttattattg gtggctgagc caagtctgtt
     4141 tgagcgttat gctgaattag cctttaatgc cggtacgttg gaacgttcga ttattctgaa
     4201 taccgaggat taccaaaaga tagttaatcc tgtgttggtt caattcgcca aaagctaggt
     4261 tatggggcga aaaactccac gattttgcca atagcgatat ccattaattt agggttagaa
     4321 aaagcgtgat ctgccccttc aatcgggagc agttcaatca cgttattatc ggcaaactgc
     4381 tgtgaagtgc ttaacgccac catttcatct tccgtgccgt gcagaattaa aatttgctcg
     4441 gcataatcca aataatcgtt ttgggtaata tcggttagct catcaaaaaa ggcttggctg
     4501 attttcatct ggcgatcaaa cccccacaaa atttctcgcc ctttggcaag tttttgccgt
     4561 tcctcttctg acaagcggtt cattaacgaa tggtagattt gtactgccgg tgcccgcaat
     4621 gcaattttcc gaaacggatt gcctttttcc gccagatatt tcaaggttaa atagccgcca
     4681 aaactagtgc cgtaggcata cagattttta gcattcaggc tggttttggc ataatcaatc
     4741 accagttgca gataagtaat ggattcctca accgagagtt ttttacgggc atcatcaccg
     4801 tggcaaggga aatcaaaggc aagggcagcg tagcctttat attttgagct aaggcgttcc
     4861 ccaaatttgg tattcgattt cagatcttta gacgaaccaa agccgtgcaa cacaatcact
     4921 acattctcga tctgctgcaa ttctttttca taaaacagtt tacagcgaat gctgagaccc
     4981 tgttcattaa tctcaaagcg tttttccatt ttatgttaag tattgaaatt gaagatgaca
     5041 aaattttagc actatttgag tgcaagcggt cggatctctc gcaaaatttg caaattttcc
     5101 ggtaaattta accgcttgta ctagccaaaa tttaagcata tttatagaat acagccttcg
     5161 tttttaaaag gagaaatgaa atgttacaac ttattttagc gattttatgt agcgtgactg
     5221 taggcgtgtt gattaaattc gcccgccaaa aagggattcg tatcgaccaa agtattgcgg
     5281 tgaactatat cgtgactacc acgatgacgt ttttattact aaaaccggat taccaagggc
     5341 agtcgattac cgatattatt gctaataacc cgacttccta tatttttgtg attttaagta
     5401 tcacattgcc tactattttc ttggtgcaag caaaagccct agagtttgcc ggtattatcc
     5461 gaaccgatgc ggcacaacgt ttatcgctgt tcctgccgat tttggcggca ttcaccattt
     5521 ttggtgagca ggttacgcaa aataaattaa ttgcacttct gttagctttc gtggcactcg
     5581 gttgcttatt atggaaaggc aatcagggaa tggaaaaagg cggaaaaatt gcgattatca
     5641 gccttgctct ggtttgggta ggctatggcg tgaacgatat tttatttaaa cagattgcca
     5701 aatcaggctc tgctttccct gtcacccttt cgattgcctt tatcggtgca ggctgtttta
     5761 tgtttatcta tttgctactt aaacgcactc aatggcacgt tccaagtgtc ttaaccggct
     5821 tattgctagg cgtgttgaac ttcggcaata ttctgttcta tattcaagct caccaagcca
     5881 tgaaagatga cccaacttta gtcttcactg gaatgaactt aggggtaatt tgcttaggca
     5941 ctttcgttgg agcattcttc tttaaagaga aaatccacaa agtgaactac ttaggcgtgg
     6001 cgattgcgat tgtggcgatt atttgcttgt tttattggcg gtaaatatac tttccaaaat
     6061 taagggcgaa cataatgttc gcccttaatt tttgagtaat atagtgtaaa atattcaata
     6121 aattcgaccg cttgttgtac cgattcatat ttcataaagg acaaaaaatg aagctaaaag
     6181 ccttatctac actcattgcc tcttcaatgc tatatagtca gagttttgct gtaccgccgg
     6241 aaaaaacagc tgaagagcca cagccctttt tcaataaaac cgaatctggg tatgatttag
     6301 tctttaatga tcaaaactat cgtttaattg aaacggaatt aaatggcgag aaaatccaat
     6361 tccgtgcctt tgagcggatt gtgtatgtga acaatccgat tgagccagat taccaaacaa
     6421 ttaatctgta tgtgccggaa gcttactaca ataatggcga aatcaatggc taccaagcgg
     6481 ataccgcccc gatcttcttg ccaaattcag tggggggcta tatgccggca aaagcggcaa
     6541 gttatgataa aaaaagacgt gacggttcac caggtacgat tttggtggcg ttatctaaag
     6601 gctatgtggt ggcaagtgtg ggagcaagag gacgcacgct acaaaaagag ggaaaataca
     6661 ccggcaaagc accttctgcc attattgatt caaaatccgc cgcccataaa gcgtgtcggt
     6721 tcgagtccga ccactggcac cactcactat gtaaaattta gttttacata gtttgctgta
     6781 agcatttgaa ttttgaatcg tttgtgtagg attgttcaaa tcatattcgc aaatacacag
     6841 ttactacgat gtatccagcc aatacatcca ttgggatttt ccgtccgaat ttcagacgtt
     6901 ctagatctta cacattcttt caagtttgta aagcctgaaa atcatttttt agattgattt
     6961 tatttgaaaa aatctatttt ttcttttaat tcaaatttag tcaaaacttc accagatatt
     7021 tttttccgaa aaaccctctc tacctatttt actgtttaca taactgctct tgcaattgct
     7081 ataaatgga
//
LOCUS       DANLUQ010000132         6995 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.133, whole genome shotgun sequence.
ACCESSION   DANLUQ010000132 DANLUQ010000000
VERSION     DANLUQ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6995
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.133"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            19..1281
                     /locus_tag="PXB27_002038"
     CDS             19..1281
                     /locus_tag="PXB27_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDL5346790.1"
                     /translation="MGTLNSFLGIIVLLFIAFLFSSNKRAINVRTVLGALALQVAIGA
                     LVLYVPAGRDALNAMATGVSKVISYGNEGISFLFGGLVSDKMFEVFGGGGFVFAFRVL
                     PVIIFFSALISLLYYIGVMQWVIRILGGLLQKALGTSKAESMSAAANIFVGQTEAPLV
                     VKPYISKMTESELFAIMAGGLASIAGSVMAGYAGMGVPLTYLIAASFMAAPAGLLFAK
                     IMVPQTEKFRDELEAVDLEKPANILDAAASGASSGMQLALNVGAMLIAFVALIALVNG
                     ILGGIGGWFGYDTLNLGQIFGWVFKPLAWVIGVPWEEAEIAGQMIGLKLAVNEFVGYL
                     EFAKYLAPESTVHLTDKTVAIITFALCGFANFSSIAILIGGIGAMAPSRRGDIARLGI
                     KAVIAGSLANLMSATIAGLFIGLGGAVL"
     gene            1426..2202
                     /gene="deoC"
                     /locus_tag="PXB27_002039"
     CDS             1426..2202
                     /gene="deoC"
                     /locus_tag="PXB27_002039"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDL5346791.1"
                     /translation="MNLKKSAEIALSLMDLTTLNDNDTDEKVIALCQQANTKFGTPAA
                     ICIYPRFIPIARKALKQQGIEQVKIATVTNFPHGNDDIEIAVAETKAAVAYGADEVDV
                     VFPYKALIAGNEQIGFDLVKQCKAVCAANNVLLKVIIETGELKSAELIRKASEISIQA
                     GADFIKTSTGKVAVNATLESARIMLETIRDLGVADRVGFKAAGGVKTSEEAAQYLALA
                     ESILGNDWVSASHFRFGASSLLANLLATLNDEQQQAVQGY"
     gene            2223..2942
                     /gene="deoD"
                     /locus_tag="PXB27_002040"
     CDS             2223..2942
                     /gene="deoD"
                     /locus_tag="PXB27_002040"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDL5346792.1"
                     /translation="MATPHINAPEGAFADVVIMPGDPLRAKYIAETFLEDAKEVTNVR
                     NMLGYTGTYKGRRISVMGHGMGIPSCSIYAKELITEYGVKKIIRVGSCGAVRNDVKVR
                     DIVIGVGACTDSKVNRIRFRDNDFAAIADFDMTLAAHQAAKAKGIEARFGNLFSADLF
                     YSPDVEMFDVMEKYGILGVEMEAAGIYAVAAEYGAKALAICTVSDHIRTGEQTTSEER
                     QLTFNGMIEIALESVLIGDKQ"
     gene            complement(3018..3869)
                     /locus_tag="PXB27_002041"
     CDS             complement(3018..3869)
                     /locus_tag="PXB27_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDL5346793.1"
                     /translation="MQTHRAIIESIFALAPKNELWATPISGLTVHAADRSIRFNGYIQ
                     DPSLCVVLLGERQICTNGHCQVFGNQEMMFCPVNLPIVTQTLSVAENQPYLAISLRLD
                     FDLIAKIITQLPPQKISENINQRTKWELENEIKDCLLRLLNLLNSPNHIHFLAPLILN
                     ELYFYLLQSEQGGYIRSLLSQDGNIAKIANATKILERKFADTLNMSALAEEVGMSVAG
                     FYAHFKSVTSMTPLQYQKSLRLNAAREKLQAGVSVSETAYQVGYESPSQFSREYKRQF
                     GESPKGR"
     gene            3984..4415
                     /locus_tag="PXB27_002042"
     CDS             3984..4415
                     /locus_tag="PXB27_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDL5346794.1"
                     /translation="MKIFYHTSATATGGRDGRTQVDDGSIGFDLVGFQNESGKVGTNP
                     EQLFAMGYAACFDSAMNHVAPTLGIKPTKSSTTVAVGIGQKADGAFGLDLDITITVEG
                     ISAEEAKTLISKAHEVCPYSNATRGNVDVRLHVKVVSEFDL"
     gene            4432..5112
                     /locus_tag="PXB27_002043"
     CDS             4432..5112
                     /locus_tag="PXB27_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HDL5346795.1"
                     /translation="MKMLDFETTVKTRQSIRKFLPLPMTETEIKQILADAQNAPSACN
                     TQPWLVHVASGETLARLKARFKKTFEAGINDADFEFNQTKFTGDYEPRWRNQYSHVFT
                     TSYGIPREDKAGRQVVYDDNIVGYGAPHMAFLFMPSIDGHDVNIASDVGMYSQTFLLS
                     LTARGFGGIPQLALAMFAKDVREELGIPEHYKLLHAISFGHPDWDAMQNKHHLGRVPV
                     EESATLHW"
     gene            5176..6300
                     /locus_tag="PXB27_002044"
     CDS             5176..6300
                     /locus_tag="PXB27_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDL5346796.1"
                     /translation="MINLFTPYTLNNDVEIKNRLVVAPMTHSASNDDGTISEQERHFL
                     HNRAADMGLFITAATGVMRNGKAFYGQPEALDERYLASLTETANILKNQGTKAILQIF
                     HGGAQAIPALLDGANAVAPSEHANGAKALTADEVKQMIQAFANATDLAIQAGFDGVEI
                     HGANGYLIQQFFSTEFNQRTDEWGGSLEKRLRFPLAVVEAAVSVREKHQRPDFIIGYR
                     FSPEEPGEHGLTMTDTYALIDALCEKPLQYLHISLWDFGKKARRGADTNFTRMQLVHQ
                     HINGRLPLIGVGNLLTGEQIRKAYATGWAEFIALGKAVMLNPNVATLLKDNRDSEIVS
                     ELDPQQADHYGIPDRLWGYCVQGGAWLPPLKGQNGQPMDI"
     gene            6384..>6995
                     /locus_tag="PXB27_002045"
     CDS             6384..>6995
                     /locus_tag="PXB27_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HDL5346797.1"
                     /translation="MITLHYLNSSCSHRIVWLLEALGLDYELKIYHREESGFAPMEVK
                     ALHPLGKAPVLQDGDLVLAEGNAIIQHLIARYAEGKFEPTRGSEAYSMYHYWLSISAS
                     LFSANLVTMLANRSDLGAFGDYAKAQTPLYFNHIEQTLSDKKWIVGDELTGADFSLCF
                     PLQWGRNFVEMANYPNIARYLEQVENHPAYQKANQKALKLDMNR"
BASE COUNT         1968 a         1411 c         1600 g         2016 t
ORIGIN      
        1 tttataatta gaggttctat gggtactctt aacagctttt taggcattat tgttttacta
       61 ttcattgcat ttttattttc gagcaataaa cgtgcaataa acgtgcgtac cgtattaggc
      121 gcattagcat tgcaggtagc tattggtgca ttggtgcttt atgtgccggc agggcgtgat
      181 gccttgaatg cgatggcaac aggtgtgtca aaagtgattt cttacggtaa tgaagggatt
      241 tcatttttat tcggtggctt agtcagcgac aaaatgtttg aagtatttgg cggcggcggc
      301 tttgtgttcg ctttccgtgt acttccggtg attattttct tctctgcact catttctttg
      361 ctttactaca ttggagtgat gcaatgggta atccgtattt taggtggttt attacaaaaa
      421 gcattaggta catctaaagc ggaatctatg tctgcggcag ctaatatttt cgttggccaa
      481 actgaagctc cattggtagt aaaaccttat atcagcaaaa tgacagaatc tgaactcttt
      541 gcgattatgg cagggggctt agcttctatt gcaggttctg ttatggctgg ttacgcagga
      601 atgggcgtac cattaacgta cttaatcgca gcctccttta tggcggctcc ggcagggtta
      661 ttattcgcca aaattatggt gccacaaacc gaaaaattcc gtgatgaact agaagcggta
      721 gatttagaaa aaccggcaaa tattcttgat gcagcagctt caggtgcttc ttcaggtatg
      781 caactggcat taaacgtagg ggcaatgtta attgcattcg tggcgttaat tgcattagtg
      841 aatggtattt taggaggtat tggcggctgg ttcggttatg ataccttaaa cttaggtcaa
      901 attttcggct gggtatttaa accgcttgcg tgggtaatcg gtgtgccttg ggaagaagca
      961 gaaattgcgg ggcagatgat cggcttaaaa ttagcggtaa atgaattcgt gggttattta
     1021 gagtttgcta aatacttagc tcctgagtca accgtacatt taacggataa aactgttgcg
     1081 attattacct ttgcactttg tggctttgcg aacttcagct cgattgcgat tttaatcggt
     1141 ggtatcgggg caatggctcc aagccgtcgt ggtgatattg cccgacttgg gattaaagcc
     1201 gtcattgctg gctcacttgc caacttaatg agtgcaacca ttgccggctt gtttatcggc
     1261 ttgggtggag cagttctcta atccagtttg tataaaacca ctagaaatag tggttttatc
     1321 tttttttatc tgacgtaaac ggttgctttt ggtttgtcta tttacaagcg gtcagatttt
     1381 tataaaattt taccaaccac cacgcattta ataaaaggag acactatgaa tttaaaaaaa
     1441 tccgctgaga ttgctctttc gttgatggat ctcaccacat taaatgacaa tgatactgat
     1501 gagaaagtca tcgcattatg ccaacaagcg aacaccaagt ttggcacacc ggcagcaatt
     1561 tgcatttatc cacgttttat cccgattgcc cgcaaagcat tgaaacagca aggtatcgag
     1621 caagtaaaaa ttgcgacagt cacgaatttc cctcacggta atgatgatat tgaaattgcg
     1681 gtagctgaga ccaaagccgc agttgcttat ggggcagatg aggtagatgt ggtattccct
     1741 tataaagcat taattgcagg taatgagcaa ataggtttcg acttggtaaa acaatgcaaa
     1801 gcagtttgtg ctgccaataa tgtgttgctt aaagtgatta ttgaaaccgg cgagttaaaa
     1861 tcagcagagc ttattcgcaa agcaagcgaa atttcgattc aagcaggggc tgattttatt
     1921 aaaacctcaa ccggcaaggt ggcagtgaat gctacccttg aatctgcccg cattatgctt
     1981 gaaaccattc gtgatttagg tgtggcagac cgtgttggct ttaaagctgc aggtggggta
     2041 aaaacctcgg aagaagccgc ccaatatctt gcacttgcgg aatctatttt aggcaatgac
     2101 tgggtgagtg catcacattt tcgcttcggt gcatcaagtt tattagctaa tttattagca
     2161 acattgaatg atgaacagca gcaagctgtt caaggttatt aattaataaa gaggaacata
     2221 aaatggctac tccacacatt aacgcacctg aaggtgcttt cgctgacgtt gtaattatgc
     2281 cgggcgatcc gcttcgtgct aaatatatcg ctgaaacctt tttagaagat gcaaaagaag
     2341 tgaccaatgt tcgcaatatg ttaggctaca ccggcactta taaaggtcgc cgcatttcag
     2401 taatggggca cggtatgggg attccatctt gctcgattta cgctaaagaa ttaattactg
     2461 aatatggcgt gaagaaaatt atccgtgtcg gctcgtgcgg tgcggtacgt aatgatgtca
     2521 aagtgcgtga tattgtcatc ggtgttgggg cttgtaccga ttctaaagta aaccgtattc
     2581 gcttccgtga taacgatttt gcggcgattg ctgattttga tatgacatta gctgctcacc
     2641 aagcagctaa agcgaaaggc attgaagctc gtttcggtaa tttattctca gccgatttat
     2701 tctactcacc tgatgtcgaa atgtttgatg taatggaaaa atacggcatt ttaggcgtag
     2761 aaatggaagc agcaggcatt tatgcggtag cggcagagta tggagcaaaa gcattagcaa
     2821 tttgcaccgt ttcagaccat atccgcaccg gtgagcaaac tacctctgaa gaacgtcaat
     2881 taacctttaa tggtatgatt gaaattgcgt tagagtcagt attaattggc gataagcagt
     2941 aatttctaac ctgacaagcg gtgaaatttt gcaaaaaatt tgcagaattt gaccgcttgt
     3001 tatttttgat tcttccttta tcttcctttc gggctttcgc caaactgcct tttatattct
     3061 cgactaaact ggcttgggct ttcatagccg acttgatagg cggtttcact cacgcttact
     3121 cctgcttgta gtttttccct tgccgcattt agtcttaacg atttctgata ttgcagtggt
     3181 gtcattgaag ttacgctttt aaaatgagcg taaaaccccg ccacgctcat tcctacctct
     3241 tcagccagtg ctgacatatt tagtgtatcg gcaaattttc tttctaagat ttttgttgca
     3301 ttggcaattt tagcaatatt gccatcttga gagagtagag agcgtatata gccaccttgc
     3361 tcagattgta ataaatagaa atagagttcg tttaatatca atggtgcaag aaaatgaata
     3421 tgatttggag aattaagcaa atttagtaag cgtaacaaac agtctttaat ttcattctcc
     3481 agctcccatt tagtgcgttg attaatgttt tcacttattt tctgtggggg aagctgcgtg
     3541 ataattttgg caataagatc aaaatccaag cgtagtgaaa tcgcaagata aggctgattt
     3601 tctgcaactg ataaggtttg ggtaacaatc ggcaaattca cagggcaaaa catcatttcc
     3661 tgattaccga agacttggca atgtccattg gtacagattt gccgctcgcc taataatact
     3721 acacataaac ttgggtcttg gatataaccg ttaaatctaa tagaacgatc ggcagcgtga
     3781 acggttagcc ctgaaatggg agtagcccaa agttcatttt tcggagcgag ggcaaaaata
     3841 gattcaataa tagctcgatg agtttgcata ataatctcac ttttagaaaa ttaggcatat
     3901 tttatagaaa atcaggcaag acgacaaata gcaaaaagcg gataatatcc ccaatcaaat
     3961 aaagcattta ataggagcaa aatatgaaaa ttttttatca cacatcagca actgcaaccg
     4021 gtggtcgtga cggcagaacc caagtggatg acggttctat cggttttgat ttagtcggtt
     4081 tccaaaatga aagtggcaaa gtaggcacta atccggaaca attattcgca atgggctatg
     4141 ctgcctgctt tgacagtgca atgaatcacg ttgccccaac cttaggcatt aagccaacca
     4201 aatccagtac cactgttgca gttgggattg gtcaaaaagc tgatggtgct ttcggcttag
     4261 atttagatat taccattact gtggaaggca ttagtgcgga agaagcgaaa actttaatta
     4321 gcaaggcaca cgaagtttgc ccatattcaa atgcaacccg tggtaatgta gatgtgcgtt
     4381 tacacgttaa agtcgtgagt gaatttgact tataaaaaat aaatagggga aatgaaaatg
     4441 ttagattttg aaaccacggt aaaaacacga caatcaatcc gcaagttttt accgcttccg
     4501 atgacggaaa ctgaaatcaa acaaattctt gccgatgcac aaaatgcccc ttcggcttgt
     4561 aatacacagc cgtggttagt acacgttgcc tctggtgaaa cgctagcacg cttaaaagct
     4621 cgttttaaaa agacttttga agcgggcatt aatgatgcgg attttgagtt caatcaaacg
     4681 aaatttaccg gtgactacga accacgttgg cgtaaccaat acagccacgt ttttaccacc
     4741 agctatggca ttccccgtga agataaagcc ggtcgccaag tggtgtatga tgacaatatt
     4801 gtcggttatg gtgcgccaca tatggcattc ctatttatgc caagcattga cggacacgat
     4861 gtcaatattg ccagcgatgt tggtatgtat tcgcaaactt tcttgctctc actcaccgct
     4921 cgtggttttg gcggcattcc gcagcttgcc cttgcgatgt ttgctaaaga tgtgcgtgaa
     4981 gagttaggca ttcctgaaca ttacaagctc ctccacgcca tttctttcgg acacccagat
     5041 tgggacgcta tgcagaacaa acaccattta gggcgtgtgc cggtggaaga gagtgcgacc
     5101 ttacattggt aataaacaag cggttgattt ttcgcaaaaa tttgcgaaat caaccgtatt
     5161 ttttaggaga atacgatgat aaatttattt accccttata ctctgaataa cgatgtagaa
     5221 attaaaaacc gccttgtagt tgccccaatg acccattccg cctccaacga tgacggcaca
     5281 attagcgagc aagaacgcca tttcttgcat aatcgtgcag cagatatggg cttatttatc
     5341 accgcagcaa ccggggtaat gcgtaacggc aaagcgtttt acggtcagcc ggaagctctt
     5401 gatgaacgct atttggcaag cctgacagaa actgccaata tcttgaaaaa tcaaggcacc
     5461 aaagcgattt tgcaaatttt ccacggtggg gcacaagcta ttcctgcctt gcttgacggg
     5521 gcgaatgcag ttgcgccgtc cgagcacgcc aacggtgcta aagccctaac ggcggacgaa
     5581 gtgaagcaaa tgatccaagc tttcgctaat gccaccgatt tagctattca agccggtttt
     5641 gatggtgtgg aaattcacgg ggcgaacggc tatttaattc aacaattttt ctctactgaa
     5701 tttaatcaac gcactgatga atggggcggt agccttgaaa aacgcttgcg tttcccgtta
     5761 gcggtggttg aggcagcggt cagcgtgcgt gaaaaacacc aacgccctga ttttattatc
     5821 ggctaccgtt tctcaccgga agagccgggc gaacacggct taaccatgac cgatacctac
     5881 gccctgattg atgctttgtg cgaaaagccg ttgcaatact tgcacatttc actctgggat
     5941 ttcggcaaaa aagcccgccg tggtgcagat accaacttca cccgtatgca actggtacac
     6001 caacacatca acgggcgttt accgctgatc ggcgtgggta acttattaac cggtgaacag
     6061 atccgtaaag cttatgcaac aggctgggca gaatttatcg ccttaggtaa agcggtaatg
     6121 ctcaacccga acgtggcgac cttgttgaaa gataatcgag acagtgagat tgtcagcgaa
     6181 ctcgacccgc aacaagccga ccactacggc attcccgata gactatgggg ctactgcgtg
     6241 caaggtggtg cttggttacc gccgcttaaa gggcaaaacg ggcaaccaat ggatatttaa
     6301 tcattacaga caagcggtca tttttttgta aaaatttgca attaagtgac cgcatttttt
     6361 caattatctg aaggagaaaa attatgatta cgttacatta cctgaacagt tcctgctctc
     6421 accgcatcgt ttggttgttg gaagccttag gcttggatta cgagctaaaa atctaccatc
     6481 gtgaagagag cggttttgca cctatggagg taaaagcctt gcacccactt ggcaaagccc
     6541 ctgtgttgca agatggcgat ttggtgcttg ccgaaggcaa tgcgattatc cagcatctga
     6601 ttgcccgtta cgccgaaggg aaatttgagc cgactcgtgg tagcgaagcc tattcaatgt
     6661 atcactactg gttgtcgatt tctgcttcat tattctcggc aaaccttgtg acaatgttgg
     6721 ctaatcgctc tgatctcgga gcttttggcg actacgcaaa agcgcagact ccgctgtatt
     6781 ttaaccatat tgagcaaaca ctttcagata aaaaatggat tgtgggtgat gaactaaccg
     6841 gtgcggattt ttctctctgt ttcccactac aatgggggcg taattttgtt gaaatggcaa
     6901 attacccgaa tattgcacgc tatttagaac aggtggaaaa tcaccctgct taccaaaaag
     6961 cgaatcaaaa agccctgaag cttgatatga ataga
//
LOCUS       DANLUQ010000133         6819 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.134, whole genome shotgun sequence.
ACCESSION   DANLUQ010000133 DANLUQ010000000
VERSION     DANLUQ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6819
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.134"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..654
                     /locus_tag="PXB27_002046"
     CDS             <1..654
                     /locus_tag="PXB27_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL5346798.1"
                     /translation="WFTASEVAGMGNLPEQPSNVTRRATKNNWLKRQIEGKRGVAFEY
                     HYSSLPISVQQELGFAQTAVKEEPRTLVAAQTADEMERVPFYNVQASAGFGAFNSEVY
                     APDDYIGLSKRWLDVRGFYLSYLAFITASGDSMYPTISDGDMLLVNRATTQPKDGKIY
                     VFRQGEQLWVKRVQGIMGGIRLISDNKELYSPIDVIFDETLDFEVIGQVVYIGHEMI"
     gene            702..3554
                     /locus_tag="PXB27_002047"
     CDS             702..3554
                     /locus_tag="PXB27_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDL5346799.1"
                     /translation="MVQAVSFSHFFAIHYFYFFLHKTKGQCTAKPLFSILLFHLFPKN
                     SAKFLLFFYFLVLMVLYSNEVANGKLLITGGTNLNGTLSAEGTSEIILSGRPTPHAYD
                     KVANKEVLIEGDWINRSFNATIFAAKDNGKLEISRNVANINGNFNLTDNATAQIGFTQ
                     GTSQACIRSDRTGVATCNINATLSETDLNSWERTKVAGNVSLADNSTFSLGSKADLTG
                     SITAQESTKVQLNDGSIANLTGLSTTGIFNGANGSTVNLSGVWTTLTNTVVDVLNVAN
                     GSQIHLNTPTATKQNTLTANTLTGNGIFKFVADLTKTASDIVTIKDTASGQFSIEIST
                     NGSAHSAQKIKLFETTNEEDFSLNLANEVRSGEYRYHLLKEGNVYYLNPEKEEEPKIT
                     QKGPSETADPLPEYTGDIEHSEKGPGETADPLPEYTGDIEHSEKGSGETAEPLPKYNG
                     NAHLIASELTAQTNAVLGVTTVLDHVIHSEGTQKQNTLTANTLTGNGIFKFVADLTKT
                     ASDIVTIKDTASGQFSIEISTNGSAHSAQKIKLFETTNEEDFSLNLANEVHSGEYRYH
                     LLKEGNVYYLNPEKEEEPKITQKGPSETADPLPEYTGDIEYSEKGPGETADPLPEYTG
                     DIEHSEKGPGETADPLPEYTGDIEHSEKGSGETAEPLPKYNGNAHLIASELTAQTNAV
                     LGVTTALDHVIHSEGTQNWKVWSKTDYQTTEHRSAGYGFNQDANVIHLGLEKALDNQV
                     TLGMILSQSKARNNFDHYYSGKGRLTMLSMYAKKTWQNGVFVAIDTGFGKASNRLTYQ
                     ANTVKLDRSVFVTGLSIGKAWESANVNIIPSFSARYHHLSSAGNQLVDAKIETNAVDL
                     LALQAGLSINKTLELNGLQIKPEIGSYFVDASHGKLRTRFNNLQIEQQMGRYFKQEAG
                     ISANYRNINAGIQAGFLTGNTLNKQRYISFKVTYEW"
     gene            complement(3629..4465)
                     /locus_tag="PXB27_002048"
     CDS             complement(3629..4465)
                     /locus_tag="PXB27_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDL5346800.1"
                     /translation="MLPHIYIGTGGYADTDLIGTLYPFGTAKEDFLFVYSQHYDTLEI
                     NSTFHAPIGQKAFEGMVQKAEGRLNFSVKLHQDFSHQRTATLDQAQLFLNALAPFTAE
                     GLLANLFVQFPTQFERMPLNRRYLAELCQWFADYPLAIEFRHPSWHIPAVFDTFAKSS
                     NLIWCNVDYPQNIGLPAFHFFANQRTAYLRLHGRNPNWWKGQSAAERHDYRYSDEELK
                     GLAELLFSRRTEFDRLYLYFENTTKSHAFYNIQTLKGFLAEKGFQIKPVPENLLGQQS
                     LF"
     gene            4573..5448
                     /locus_tag="PXB27_002049"
     CDS             4573..5448
                     /locus_tag="PXB27_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL5346801.1"
                     /translation="MWIWFTLLAAVAQAGRNAFQKQLSVNVPVLGVTLARFFYAVPLA
                     SGYLYFLYQYDDNLNIPHFPPLFFVYAIGAGLAQILATSLMVQLFHLKNYAIGVGLAK
                     SEAVWAALLGVLFFGVTISGIGWLGVVLGGVAIFLMTGSGNLRQLSWKTLFLGIASGL
                     CFALTSLWVREASLQLNSHYLLSAAWVLFSVISFEAVILVIYLALLQKETLIKFFRFP
                     KLGMLTSLFSFLGSFGWFNAMSLNHVAFVKTLGQVEIFFILGISYFVFKERLKIQDFI
                     GLILVVIAAILVVLG"
     gene            5584..6024
                     /gene="nudB"
                     /locus_tag="PXB27_002050"
     CDS             5584..6024
                     /gene="nudB"
                     /locus_tag="PXB27_002050"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HDL5346802.1"
                     /translation="MNYKNPHSILVVIYAKNTQRVLMLQRKDDPDFWQSVTGSINIGE
                     TPLEAAEREVSEETGLQTFEQKQPLVDCQKQIEFEIFPHFRYKYAPNITHCVEHWFLL
                     PLDSEQEPILTEHLAYRWVSVEDAVRLTKSPNNAQAIQEYLTSK"
     gene            6037..6777
                     /locus_tag="PXB27_002051"
     CDS             6037..6777
                     /locus_tag="PXB27_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL5346803.1"
                     /translation="MAGHSKWANIKHRKAAQDAQRGKIFTKLIRELVTAAKLGGGDTS
                     SNPRLRAAVDKALSSNMTRDTINRAIERGVGGGDDTNMETKIYEGYGPGGTAVMVECL
                     SDNANRTISQVRPSFTKCGGNLGTEGSVGYLFSKKGLILVANADEDALTEAAIEAGAD
                     DIQPQEDGSFEIYTAWEDLGSVKDGIEAAGFKIENAEVTMIPSTTVELDAETAPKLLD
                     LINRLEDCDDVQNVYHNGEISDEVAALL"
BASE COUNT         2105 a         1356 c         1472 g         1886 t
ORIGIN      
        1 tggtttacgg catctgaagt agctggaatg ggaaatttac ctgaacaacc atcaaatgta
       61 acaagaagag ctaccaaaaa taattggctc aaaagacaaa tagagggaaa aaggggggtt
      121 gcttttgaat accactacag ctctctccca ataagcgtgc agcaagagtt aggctttgct
      181 caaacggcag taaaagaaga gcctagaaca ttagtggcag cccaaactgc agatgaaatg
      241 gaacgtgtgc cgttttataa tgtacaggct tcagcaggct ttggtgcttt caatagcgag
      301 gtgtatgccc ctgatgacta tattgggcta agtaaacgat ggctagatgt gcgtggcttc
      361 tatttgagct atcttgcatt tattaccgct tcaggtgatt caatgtaccc aaccatcagt
      421 gatggcgata tgctattggt aaaccgagcc accacccagc ctaaagacgg taaaatttat
      481 gtgttccgcc aaggcgagca gctttgggtt aaacgtgtgc aaggcattat gggcggtatt
      541 cgcttaatca gcgataataa agagctatac agccctattg atgtgatttt tgatgaaacg
      601 ttagattttg aggttatcgg gcaggttgtg tacattggac acgagatgat ttaaacgcca
      661 tttaaagcta cttaaatcat ttttactttt tcgttgtttt aatggtacaa gcggttagtt
      721 ttagccattt tttcgcaatt cattattttt acttttttct acataaaaca aaggggcaat
      781 gcaccgcaaa gcccctgttt tcaatacttc tgtttcactt attcccgaaa aattccgcca
      841 aattcctatt attcttttat ttcttagttt taatggttct atacagtaat gaagtagcaa
      901 acggcaaatt gcttatcact gggggaacga acctcaacgg tacgctttct gcagaaggta
      961 caagcgaaat cattttatcg ggccgtccaa cacctcatgc ctatgataaa gtagcgaata
     1021 aagaagtcct gattgaaggc gactggatca accgatcgtt caacgcaaca atctttgcag
     1081 ctaaagacaa tggcaagctt gaaatcagcc gtaatgttgc caatattaac ggtaatttta
     1141 atttaacaga taatgctacc gctcaaattg gttttaccca aggtacctca caagcatgta
     1201 ttcgctctga ccgcacaggc gtagcaacct gtaatattaa cgctacttta tcagagactg
     1261 atttaaacag ctgggagaga accaaagtgg ctggcaatgt ctctttagct gacaattcaa
     1321 cattttcgtt aggcagtaaa gcagacttaa ctggctcaat tacagctcag gagtccacta
     1381 aggttcaact taatgatgga agtattgcta atttaaccgg cttgagtaca acaggaatat
     1441 ttaatggcgc caatggttca accgttaatc tttctggggt atggacaacc ttaaccaaca
     1501 ctgttgtaga tgtcttgaat gtcgcaaatg gtagccaaat ccatttaaac actccaactg
     1561 caacaaaaca gaatacgctt accgctaata ccctcacagg gaacggcatc tttaaatttg
     1621 tcgctgatct gacgaaaaca gcgagcgata tagtcacaat taaagacacc gcaagcggtc
     1681 aattttcgat agaaattagc acaaatggta gcgcccattc agcccaaaag attaaattat
     1741 ttgaaacaac aaatgaagaa gacttttctc ttaatttagc aaatgaagtg cgctcaggcg
     1801 aatatcgcta ccatttactc aaagaaggga atgtgtatta cctcaatcca gagaaagagg
     1861 aagaacctaa aattactcaa aaagggccaa gtgaaaccgc tgacccatta cctgaataca
     1921 ccggggatat tgagcattct gaaaaagggc cgggtgaaac tgctgatccg ttacctgaat
     1981 acacaggcga cattgagcat tcagaaaaag gatcgggtga aaccgctgag ccattaccta
     2041 aatacaatgg aaatgcacat ttgatcgcat ccgagttaac tgcgcaaact aatgctgtat
     2101 taggtgtaac tacagtatta gatcatgtaa ttcattcgga agggacccaa aaacagaata
     2161 cgcttaccgc taataccctc acagggaacg gcatatttaa atttgtcgct gatctgacga
     2221 aaacagcgag cgatatagtc acaattaaag acaccgcaag cggtcaattt tcgatagaaa
     2281 ttagcacaaa tggtagcgcc cattcagccc aaaagattaa attatttgaa acaacaaatg
     2341 aagaagactt ttctcttaat ttagcaaatg aagtgcactc aggcgaatat cgctaccatt
     2401 tactcaaaga agggaatgtg tattacctca atccagagaa agaggaagaa cctaaaatta
     2461 ctcaaaaagg gccaagtgaa accgctgacc cattacctga atacaccggg gatattgagt
     2521 attctgaaaa agggccgggt gaaactgctg atccgttacc tgaatacaca ggagacattg
     2581 agcattcgga aaaagggccg ggtgaaactg ctgatccgtt acctgaatac acaggagaca
     2641 ttgagcattc agaaaaagga tcgggtgaaa ccgctgagcc attacctaaa tacaatggaa
     2701 atgcacattt gatcgcatcc gagttaactg cgcaaactaa tgctgtatta ggtgtaacta
     2761 cagcattaga tcatgtaatt cattcggaag ggacccaaaa ttggaaagta tggtctaaaa
     2821 cagattatca aacgacagag catcgctctg ccggatatgg atttaaccaa gatgcgaacg
     2881 ttatccatct aggattagag aaagccttag ataatcaggt cacacttggt atgatattat
     2941 ctcaatcaaa agcgagaaat aattttgacc attattatag cggtaaaggt cgcttaacaa
     3001 tgctatcaat gtatgcgaag aaaacatggc aaaatggtgt ttttgttgcg atagataccg
     3061 gttttgggaa agcaagcaat cgcttaactt atcaagcaaa tacagtaaaa ttagatcgtt
     3121 cagtatttgt aacagggctt agtatcggta aagcatggga atctgcaaac gtgaatataa
     3181 tcccatcatt tagcgcccgt tatcatcatt tatcttcagc aggtaatcaa cttgtagatg
     3241 caaaaatcga aacaaacgct gttgatcttc tcgctcttca agctggtctg tcaattaata
     3301 aaacgcttga actaaatggc ctgcaaataa aaccggaaat aggtagctat tttgttgatg
     3361 caagccatgg gaaattaaga acacgtttca ataatttaca aatagagcag caaatggggc
     3421 gttattttaa acaagaagca ggtatatccg ctaattatcg caatataaac gcaggaattc
     3481 aggcaggttt cttaacgggg aatacgctaa ataaacagcg ttatatctcg ttcaaagtta
     3541 catatgaatg gtaaatattg agattaaggg ctatccatta ctaaatggca tggcccttta
     3601 tttactcctc aacaatgtaa attacccatc aaaacaaact ctgctgccca agcaaattct
     3661 ccggcacagg tttgatttga aatccttttt ccgccaaaaa gccttttagg gtttgaatat
     3721 tataaaaggc gtggctttta gtggtgtttt caaagtagag atagagcctg tcaaattccg
     3781 ttctgcggct aaaaagcaat tctgccaagc cttttaattc ctcgtctgaa tagcggtaat
     3841 cgtggcgttc tgccgcactc tgccctttcc accaattcgg gttacgacca tgtaagcgta
     3901 aataagcagt acgttggttg gcaaaaaagt gaaatgccgg taagccgata ttttgtgggt
     3961 aatccacatt acaccaaatc agattagagc ttttcgcaaa ggtatcaaac accgccggaa
     4021 tatgccagct tgggtggcga aattcaatcg ctaacggata atccgcaaac cattggcaca
     4081 actcggcaag gtaacggcgg ttaagtggca tacgttcaaa ttgagttggg aactgaacaa
     4141 ataaatttgc caataaccct tcagcggtga acggagctaa cgcattaaga aataattgtg
     4201 cttgatctaa agtcgccgtg cgttgatggc tgaaatcttg atgcagtttc accgaaaaat
     4261 tcagccgacc ttccgctttc tgcaccattc cttcaaaggc tttctgcccg atgggggcgt
     4321 ggaatgtgct gttaatttcc agcgtgtcgt aatgttggct atagacaaag agaaaatcct
     4381 cttttgcagt gccgaatggg taaagcgtgc cgattaaatc tgtgtcggca tagccgccgg
     4441 tgccaatata aatatgaggg agcattgtgt ttattctttc aattttttgc aaattttaat
     4501 ggttttttaa ccgcttgcaa actgagaata aaagagtaag atttggctat cttttcttat
     4561 ttcatcatca ttatgtggat ttggtttact ctgctggcag ccgtggctca agccggtcgc
     4621 aatgcctttc aaaagcaatt aagcgtcaat gtgcctgtat tgggggtaac gctcgcccgc
     4681 tttttctatg ctgtgccgtt ggcaagcggt tatctctatt ttttatacca atatgacgat
     4741 aacctcaaca ttccccattt tccaccgctg ttttttgtat atgctatcgg ggctgggttg
     4801 gctcaaattc tggcaacttc gctaatggtg caactttttc acttaaaaaa ttatgcaata
     4861 ggtgtcgggc tggcgaaaag cgaggcagta tgggcggctt tactgggcgt gttatttttt
     4921 ggtgtaacca tcagtggcat tggctggctt ggcgtggtac taggtggagt ggcgattttc
     4981 ttaatgaccg gcagcggtaa tctacgccaa ctttcgtgga aaacactctt tctcggcata
     5041 gccagcggtt tatgtttcgc cctcacatca ctttgggtgc gagaggcaag cctgcaactc
     5101 aacagccact atttgctttc agcggcgtgg gtacttttta gcgtgattag ctttgaggca
     5161 gtgatattgg ttatatacct agctcttctc caaaaagaga cactgattaa attctttcgt
     5221 tttccaaaat taggtatgtt gaccagcctg ttttcatttc tcggctcatt cggttggttc
     5281 aatgcaatga gcctgaacca cgttgccttt gtaaaaacgc ttggacaagt ggaaattttc
     5341 tttattctcg gtatttccta ttttgtgttt aaagaacggc ttaaaattca agattttata
     5401 gggctgattt tagtagtgat tgccgcaatc ttggtggtgc tgggctagat cggattttta
     5461 cgttttttat ctagctttgt agggtgggcg taagcccacg caaaaccaat aaatcattta
     5521 tccgtgggct tacgcccacc ctacgatacc taaaaaaatt aaatttcaca tcttagtaat
     5581 aaaatgaact ataaaaatcc tcatagtatc cttgtggtga tttatgctaa aaatacccaa
     5641 cgagtattga tgctacaacg caaggacgat cctgattttt ggcaatcggt gaccggttcg
     5701 atcaatattg gagaaacacc gctggaagca gcagaacggg aagtgagcga agaaaccggt
     5761 ttgcaaactt ttgagcaaaa acaaccgctt gttgattgcc aaaagcagat agaatttgaa
     5821 attttcccgc attttcggta taaatacgca cctaatatca cacattgtgt cgaacattgg
     5881 tttttgctgc cacttgatag tgagcaagag cctattttaa ccgaacattt ggcatatcgt
     5941 tgggtttcag tggaagatgc ggttcgactg actaaatctc ccaacaatgc ccaagcaata
     6001 caagaatatt taacatcaaa ataaggaaac aacagaatgg caggccatag taagtgggca
     6061 aatattaaac accgtaaagc ggcacaagat gcacaacgcg gtaaaatttt tactaaatta
     6121 attcgtgaat tggtgactgc cgccaaactc ggcggtggtg atacctcttc caaccctcgt
     6181 ttgcgtgcgg cggtggataa agcgttatcc agcaatatga cccgtgatac catcaaccgt
     6241 gcaatcgagc gtggtgtagg cggtggcgat gataccaata tggaaaccaa aatctacgaa
     6301 ggttacggcc cgggtggtac agcagtgatg gtggaatgtt taagtgataa cgctaaccgt
     6361 acgatttccc aagttcgtcc aagtttcaca aaatgtggcg gtaacctagg tactgagggt
     6421 tcggtaggtt atttatttag caaaaaaggc ttaattttag tggcaaatgc cgatgaagat
     6481 gccttaactg aagctgctat tgaggccggt gcggacgata ttcaaccaca agaagatggt
     6541 agctttgaaa tttataccgc ttgggaagat ttaggctcag tgaaagacgg cattgaggct
     6601 gcaggcttta aaattgagaa tgcagaagta acaatgattc catcaactac cgttgaactt
     6661 gacgcagaaa ctgcaccaaa acttcttgat ttaatcaacc gccttgaaga ttgtgatgat
     6721 gtacaaaacg tttatcacaa tggtgaaatc agtgacgaag tcgctgcatt actgtaataa
     6781 aaaaatcacg caaaataagc ataatttagg ttatgctta
//
LOCUS       DANLUQ010000134         6782 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.135, whole genome shotgun sequence.
ACCESSION   DANLUQ010000134 DANLUQ010000000
VERSION     DANLUQ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6782
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.135"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..442
                     /locus_tag="PXB27_002052"
     CDS             <1..442
                     /locus_tag="PXB27_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL5346804.1"
                     /translation="WNNVIEDAKFETSQNNIIFIIKQNIKNPESYSINGAVGLLASAL
                     ENLIRNGQKYAKKMLSVSMNVEDGHLFLTVEDDGEGVPENEYENILKPFYRVDEARAR
                     ETGGAGLGLAIVQNVVKQHQGTIHLDKSNMGGLKIELKLPLWRS"
     gene            492..2414
                     /locus_tag="PXB27_002053"
     CDS             492..2414
                     /locus_tag="PXB27_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HDL5346805.1"
                     /translation="MKLIEIENLNKYFGLGQTRAHILKNINLSIERGDFVAIIGASGS
                     GKSTLMNIIGCLDTASSGICRIDGKETQTMSADELSDLRQRKLGFIFQRYNLLSALNA
                     NENVALPAIYAGMDSSSRKKRADTLLTKLGLSDKTQHKPNELSGGQQQRVSIARALMN
                     GGEIILADEPTGALDSKSGETVLEILRDLHYEGHTIIMVTHDPNIAAQASRIVEIKDG
                     QIIRDERQRTDVQETALNKTTTQKPRFWNQLFESFKMATSAIMAHKMRAILTMLGIII
                     GITSVISVVALGRGSQQQILSNINNLGTNTMDIMNGTGFGDRRANLTKNLTISDALML
                     SRQNYVESTTPSSNVSATLIYGNTAVTGSVRGVGEQFIDVKGLKLISGRFFSAEEVKN
                     VAQVIVIDPNTQKDLGLTEPVEGKTILVDKKPLQIIGVAQQSNNMNQSSLTLWAPYTT
                     IMQRISGAKQIDSLTVKIKDDIESQTAEKSIAELLVAKHGKKDFFIMNSDTIKQTITS
                     TTNTMTLLISSIALISLIVGGIGVMNIMLVSVTERTREIGVRMAIGAKQRNILQQFLI
                     EAVVICLIGGVIGILLASAIIWAFNTLGTNFKMMLSPESVIIAVLCSTLIGVVFGYIP
                     ARNAAQLNPITALAQE"
     gene            2498..3268
                     /locus_tag="PXB27_002054"
     CDS             2498..3268
                     /locus_tag="PXB27_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDL5346806.1"
                     /translation="MTITNTELEQILNTKLNSSAINDYAPNGLQVEGKREIKKIITGV
                     TATLPLIEKAIEKNADAILVHHGYFWKSENPCIRGMKGKRIKQLLVNDINLFGYHLPL
                     DIHSELGNNAQLAKHLGVKNLQGLEDRPNSIPMYGELENPISANELKQKLEKTLQRTV
                     ILCDEFVHSPQKMIKTIGICSGGGQGYIDLAFEKGCDAFISGEISEQTTHSAREQGIY
                     YFACGHHATERDGVKALGEWLAVEYGLDVEFIDIDNPA"
     gene            complement(3349..4479)
                     /gene="nrdB"
                     /locus_tag="PXB27_002055"
     CDS             complement(3349..4479)
                     /gene="nrdB"
                     /locus_tag="PXB27_002055"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HDL5346807.1"
                     /translation="MAYTTFSQHKNDQLKEPMFFGQNVNVARYDQQKYEIFEKLIEKQ
                     LSFFWRPEEVDVSQDRIDYAQLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWIETWTFSETIHSRSYTHIIRNIVNDPSIVFDDIVTNEEIIKRAKDISAYYDD
                     LIRDSQLYSLYGEGSYQVDGKTCEVSLRSLKRQLYLCMMSVNALEAIRFYVSFACSFA
                     FAERQLMEGNAKIIKFIARDEALHLTGTQHILNIMAYGNDDPEMAEIARECRQEAYEL
                     FLSAAEQEKAWAEYLFKDGSMIGLNRDILVQYIEYITNIRMQAVGLPLPFKARSNPIP
                     WINAWLVSDNVQVAPQEVEVSSYLVGQIDAKVDSRDFGDFEL"
     gene            complement(4506..6776)
                     /gene="nrdA"
                     /locus_tag="PXB27_002056"
     CDS             complement(4506..6776)
                     /gene="nrdA"
                     /locus_tag="PXB27_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018356743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HDL5346808.1"
                     /translation="MNKSLMVTKRNGTREQLNLDKIHRVITWAAEGLENVSVSQVELR
                     SHIQFYEGIRTSDIHETIIKAAADLITKDTPDYQYLAARLAIFHLRKKAYGQFEPPHL
                     YEHVKKLVALGKYDADLLSDYTPEEWETMNGFIDHWRDMTFSYAAVKQLEGKYLVQNR
                     VSGEIYESAQFLYLLVAACLFSKYPTASRLDYIRRFYDATSTFKISLPTPIMAGVRTP
                     TRQFSSCVLIECDDSLDSINATSAAIVKYVSQRAGIGINAGAIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATVYFPIWHLEAESLMVLKNNRGVEENRARHMD
                     YGVQINKMMYQRLIKGGEISLFSPSDVPGLYEAFFADQAKFEQLYLQYEQDPSIRQRK
                     VKAVELFSLLMQERASTGRIYIQNVDHCNTHSPFNPRVAPIRQSNLCLEIALPTKPLA
                     HFYDEQGEIALCTLSAFNLGTLSNLDELEELADLVVRALDALLDYQDYPVLAARNSSL
                     NRRALGVGVINYAYYLAKNQVRYSDGSANNLTHRTFEAIQYYLLKASMNLAKEQGACA
                     YFDQTTYAKGLLPIDTYKKEIDELTQEPLHYDWEQLRQDILQFGLRNSTLTALMPSET
                     SSQISNATNGIEPPRGYISVKASKDGILKQVVPEYERLKAYYELLWDIPNMDGYLHLV
                     GIMQKFVDQAISANTNYDPNRFAEAKVPMKVLLKDLLTAYKYGLKTLYYQNTRDGAED
                     VQDDLDEGCAGGACKI"
BASE COUNT         2144 a         1391 c         1303 g         1944 t
ORIGIN      
        1 ttggaataat gtaattgaag atgctaaatt tgaaacctca caaaataata ttatttttat
       61 catcaagcaa aacatcaaaa accccgaatc ttattctatt aacggggccg taggtttact
      121 ggctagtgct ttagaaaatt tgatccgaaa cggccaaaaa tatgcaaaaa aaatgctgtc
      181 ggtctcaatg aatgtagaag atggtcattt atttctcaca gttgaagatg atggcgaggg
      241 ggttcctgaa aacgaatatg aaaatattct taaacctttt taccgcgtag atgaagcaag
      301 ggcaagagaa acaggaggag ccgggctagg attagcgatt gtgcaaaatg tagttaaaca
      361 gcaccaaggt actattcatt tagataaaag taatatggga ggattaaaaa tcgaactgaa
      421 acttcccctt tggcgttcct aaggcaaaaa gcatatcgtt tgtaaaaaac agtcgaaaaa
      481 taaccgcttg tatgaaatta attgaaatag aaaatctcaa taaatacttt ggcttggggc
      541 aaacccgagc ccatattcta aaaaatatca atctcagtat tgaacggggt gattttgtcg
      601 caattatagg tgcttcaggt tctggcaaat ctaccttaat gaacattatt ggctgtttag
      661 atactgccag ctcaggtatt tgccgtattg atggcaaaga gacccaaaca atgtctgcag
      721 atgaactgtc tgatttacgc caacgtaaac tggggtttat tttccaacgc tataatctcc
      781 tttcagcttt gaatgcaaac gaaaatgttg ccctacctgc aatttacgcc ggaatggata
      841 gctcatcacg caaaaaacga gctgatacgt tattaacgaa gttaggatta tcagataaaa
      901 cccaacataa gccgaatgag ttatctgggg ggcaacagca acgagtcagc attgcacgag
      961 ccttaatgaa cggcggtgaa atcattcttg cagacgagcc taccggtgca ttagattcta
     1021 aaagtggcga aactgtattg gaaatcttgc gagatctaca ctacgaaggt cataccatta
     1081 ttatggtaac acacgaccca aacattgccg ctcaagccag ccgtatcgtt gaaattaaag
     1141 atgggcaaat cattcgagat gaacgccaac gtactgatgt tcaagaaacg gctctcaata
     1201 aaacgacaac acaaaaacct cgcttttgga atcaactgtt tgaatccttc aaaatggcaa
     1261 ccagtgcaat tatggcgcat aaaatgcggg caatcctcac gatgctgggc attattattg
     1321 gcattacctc tgtgatttcc gtagtcgctc tcggtcgagg ctcgcaacaa caaatccttt
     1381 ctaatatcaa caacctaggc acaaatacga tggatattat gaatggaacc ggctttggcg
     1441 atagaagagc caatctgact aagaatttaa ccatcagtga tgccttaatg ttgagtcggc
     1501 aaaactatgt ggaaagcact actccttcaa gcaatgtcag tgccacattg atttatggta
     1561 ataccgcagt aaccggttca gtacgaggtg ttggagagca atttattgat gtgaaaggat
     1621 taaagctgat ttcagggcgc tttttcagtg ccgaggaagt aaaaaatgta gcgcaggtta
     1681 ttgtaattga ccctaacact caaaaagatt tggggctaac agagcctgtt gaaggtaaaa
     1741 cgattttagt ggataaaaaa ccgttacaaa tcattggtgt ggcgcaacag tcaaacaata
     1801 tgaaccaaag tagccttacc ttatgggcgc cttataccac tattatgcaa cgcatttcag
     1861 gtgcaaaaca gatagattca ctcacggtta aaattaaaga tgatattgaa agccaaactg
     1921 ccgaaaaaag cattgccgaa ttgttagtag ctaaacacgg caaaaaagat ttctttatta
     1981 tgaatagtga tacgatcaaa caaaccatta ccagtaccac caatacaatg actttgctga
     2041 tctcctctat cgcccttatt tcgctgattg tcggtggtat cggggtaatg aatattatgc
     2101 tcgtttccgt caccgaacgc acccgagaaa ttggtgtccg aatggcgatt ggcgcaaaac
     2161 aacgcaatat tttgcaacaa tttttaattg aggcggtggt tatttgccta ataggtggtg
     2221 tgattggcat tttactggca agcgctatta tttgggcatt taatacctta ggtacgaatt
     2281 ttaaaatgat gctttcaccg gaatcggtga ttattgccgt gctctgctct acacttatcg
     2341 gtgtggtatt tggctacatt ccggcaagaa acgccgccca attaaaccca attactgctc
     2401 tggcacaaga gtaaatgcga taactatctg ctacactgta ggggcaaaaa attttttgcc
     2461 cctacatcca ttattatgcc aagtttaagg agataaaatg acaataacca atactgaact
     2521 agaacaaatt ctgaacacca aactcaacag ctccgccatt aacgactatg ccccaaatgg
     2581 cttgcaagtt gaagggaaaa gagagatcaa aaaaattatt accggtgtga cagctacact
     2641 tcccctgatt gaaaaagcga ttgagaaaaa cgccgatgca attttagtcc accacggtta
     2701 tttttggaaa agtgaaaacc cgtgcattcg aggaatgaaa ggtaaacgca ttaaacagct
     2761 tttagtgaat gacatcaatc tctttggtta tcacctacca ctggatattc actccgaact
     2821 aggtaacaac gcccagcttg ctaagcattt aggagtaaaa aatctacaag gtttagaaga
     2881 cagacctaat tcaattccaa tgtacggtga attagaaaat ccgatttccg ccaatgaact
     2941 gaaacaaaaa cttgaaaaaa cgttacaacg caccgttatt ttatgtgatg aatttgttca
     3001 tagcccacaa aaaatgatta aaacaatagg gatttgctcc ggcggaggac aaggctatat
     3061 tgatttagcc tttgaaaaag gctgtgatgc ctttattagt ggcgaaattt ccgaacagac
     3121 cactcactct gcccgtgaac aaggaatcta ttattttgct tgcggtcatc acgccacgga
     3181 acgagatggc gtaaaagcct taggtgaatg gttagccgtt gaatatggtt tagatgttga
     3241 atttattgat attgataacc ctgcataaaa aatacaagcg gttgaatttc ctgaaaattt
     3301 tgcaatttgc aaaaaatcac taaaaatcaa ccgcttgtat atcatagtct aaagttcaaa
     3361 atcgccaaaa tcacggctat ctactttagc atcaatctgg ccaaccaaat aagaactgac
     3421 ttccacttct tgtggtgcta cctgtacatt atcggaaacc aaccacgcat tgatccacgg
     3481 aatggggttg gaacgtgcct taaatggtaa tggtagtcca acagcctgca tccgaatatt
     3541 agtgatatat tcaatatatt gcaccaaaat atcccgattt agccctatca tcgaaccgtc
     3601 tttaaacagg tattccgccc acgctttttc ttgttcagct gcacttaaaa aaagctcgta
     3661 ggcctcttgg cgacattcac gtgcaatttc tgccatttca ggatcatcat tgccataggc
     3721 cataatattc aatatatgct gagtgccggt aagatgtaat gcttcatcac gtgcaataaa
     3781 tttgatgatt ttggcatttc cttccataag ctgccgttcc gcaaaggcaa atgagcaagc
     3841 aaaagagaca taaaaacgaa ttgcttcaag cgcattcaca ctcatcatac agagataaag
     3901 ttgtcttttt agactacgca aactgacctc acaggttttt ccatcaacct gataactgcc
     3961 ttcaccatac aaactataga gttggctgtc acgaatcaaa tcatcataat aggcggaaat
     4021 atctttggca cgcttaataa tttcttcgtt tgtgacaata tcatcaaata ctattgatgg
     4081 atcattgaca atattacgga tgatatgtgt gtaagaacga ctgtgaattg tttcgctaaa
     4141 tgtccaagtt tcaatccagg tttcaagctc ggggatagac actaatggca aaagcgccac
     4201 attcgggcta cgcccttgaa tggaatccaa caaggtttgg tattttaaat tgctgataaa
     4261 aatgtgcttt tcatgttcag gcagttgggc atagtcaatg cggtcttgtg acacatccac
     4321 ttcttccggt cgccagaaaa atgagagttg tttttcaatc agtttttcaa aaatttcgta
     4381 tttttgttga tcataacgcg ccacattcac attttgtcca aaaaacatgg gttcttttag
     4441 ttgatcattt ttatgttgtg aaaacgtagt atatgccatg ttattctcct ttatttctgt
     4501 tcttattaaa ttttacaagc accaccagca cagccttcat ctaagtcgtc ttgcacatct
     4561 tctgctccat cacgggtatt ttgatagtaa agggttttta acccgtattt atacgccgtt
     4621 aatagatctt ttaataatac tttcatcggt actttagctt ctgcaaagcg gtttgggtcg
     4681 taatttgtat tcgccgaaat cgcttggtca acaaatttct gcataatccc aaccaaatgc
     4741 aaatatccgt ccatattagg aatatcccaa agcagttcat agtatgcttt taaacgttca
     4801 tactccggca caacctgctt taaaataccg tcttttgatg ctttaacact aatatagcca
     4861 cgcggaggtt caattccatt ggtggcattc gaaatttgcg acgaagtctc cgatggcatt
     4921 aaggcagtta aagtggaatt gcgcaagcca aattgcagaa tatcctgacg tagttgttcc
     4981 caatcataat gaagcggttc ttgagttaat tcatcaattt cttttttata agtatcgata
     5041 gggagtaacc ctttggcata agtggtttga tcaaaataag cacacgcccc ttgttctttg
     5101 gctaaattca tcgatgcttt taataaataa tactgaatcg cttcaaaggt acggtgagtt
     5161 aaattattag cactgccatc ggaataacgc acttggtttt tagctaaata gtaagcatag
     5221 ttaatcacgc ctacacctaa tgcgcgacga tttaatgagc tgttacgtgc agctaatacc
     5281 ggataatctt gataatctaa tagggcatct agcgcacgaa ccactaaatc agccaattct
     5341 tctaactcat ctaaattgct gagtgtgcct aaattaaatg ccgataatgt acataatgcg
     5401 atttcccctt gttcatcata aaaatgtgcc agtggcttag ttggcaaagc aatttctaag
     5461 cagaggttcg attggcgtat cggtgcgaca cgaggattaa atgggctatg agtattacaa
     5521 tgatcgacat tctgaatata aattcgccct gttgaagccc gctcttgcat cagtaatgaa
     5581 aacaattcta cagctttaac tttacgttgc cgaatgctcg gatcttgctc atattgcaaa
     5641 taaagttgct caaatttagc ttgatcagca aaaaacgctt catataaccc cggcacatct
     5701 gatggactaa ataaactaat ctcaccgcct ttaattaaac gttgatacat cattttatta
     5761 atctgtaccc cgtaatccat atggcgagca cggttttctt ccacaccacg gttatttttt
     5821 aataccatta agctttctgc ttccaaatgc caaatcggaa aatacacggt agccgctcca
     5881 ccgcgaacac caccttgaga gcaagacttt actgcagttt gaaaatgttt ataaaaggga
     5941 atacagccgg tatgaaatgc ttctccacca cgaatggggc tacctaatgc ccgaattgcg
     6001 ccggcattaa taccgatccc agcacgctgt gaaacatatt taacgattgc agccgaggtt
     6061 gcattgatgg aatctaagct atcatcacac tcaattaaca cacaggaact aaattgacga
     6121 gtaggtgtac gcactcctgc cataatcggt gttggcaaag aaattttgaa agtggaagtc
     6181 gcatcataaa aacggcgaat ataatctaag cggcttgctg ttggatactt agaaaataaa
     6241 caggcagcta cgagtaaata caaaaattga gccgattcat aaatctctcc gctaacacga
     6301 ttctgcacta aatattttcc ttccaactgc tttactgctg cataggaaaa cgtcatatcc
     6361 cgccagtgat caataaatcc attcattgtc tcccattctt cgggcgtata gtcgctcagc
     6421 aagtctgcat catatttacc tagcgcaact aattttttaa cgtgctcata taaatgcggt
     6481 ggctcaaatt gtccataagc ttttttgcgt aagtggaaaa tcgccaagcg tgcggctaaa
     6541 tattgataat ctggcgtatc cttagtgatt agatcagctg cagccttgat aatagtttca
     6601 tgaatatctg aagtgcgaat accttcataa aattgaatgt gagaacggag ttctacttgt
     6661 gaaacagaaa cattctctaa cccttctgcc gcccaagtaa tcacacgatg aattttatct
     6721 agattgagtt gctcgcgcgt gccattacgc tttgttacca ttaaactttt attcataatt
     6781 at
//
LOCUS       DANLUQ010000135         6658 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.136, whole genome shotgun sequence.
ACCESSION   DANLUQ010000135 DANLUQ010000000
VERSION     DANLUQ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6658
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.136"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            244..1161
                     /locus_tag="PXB27_002057"
                     /pseudo
     CDS             244..1161
                     /locus_tag="PXB27_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014167835.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(1163..1831)
                     /locus_tag="PXB27_002058"
     CDS             complement(1163..1831)
                     /locus_tag="PXB27_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HDL5346809.1"
                     /translation="MKYLYALIFSLFFSSVYAAERQLQCKVVGISDGDTLTCLVNKTP
                     LKIRLQHIDAPEQAQPYGNKAKQALAQFVFQQNITAIVSGYDRYQRLLATLYLNDKNI
                     NLALVENGMAWAYTTKREYIEAQDQAQQAKIGLWQDKFPINPSQWRKTHTSTLPQAVK
                     KPENIANQPPTIDCQIKLSCNQIGNYAQAKRYFQQCGWKELDGNNDSIPCNKLYRQEQ
                     KNRN"
     gene            complement(1875..3242)
                     /locus_tag="PXB27_002059"
     CDS             complement(1875..3242)
                     /locus_tag="PXB27_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HDL5346810.1"
                     /translation="MSIDTILNQINSFVWGPPLLILISGVGLYLTFSLKGIQLSQLGQ
                     AFRYMFKPEKGKGQSGDISAFAALSTALAATIGTGNIVGVATAIHSGGPGALFWMWLI
                     ALFGMATKYAEGLLAIKYRTKDRAGFVAGGPMYYIELGMGKKWRWLAKAFAFFGVLVA
                     LLGIGTFPQVNGITHALESTFDVPIIFSAIILTLIVTAIILGGVQRISKIATIVVPFM
                     AIGYVIAALSVILLNMEKVPQAIITIVHAAFNPQAALGGAVGFTVMQAIQLGVARGIF
                     SNESGLGSAPIAAAAAQTKEPVRQGLISMTGTFLDTIIVCTMTGLVIVISGAWAGDVK
                     GAELTTTAFTQGLNSSFGAIIVTVGLVFFAFTTILGWCYYGERCFVYLTRGRTQYIKL
                     YRLLFIALIASAPFLQLDTIWTLADIVNGLMAFPNLIALIALRKVIISETKDFFARLK
                     TGKAE"
     regulatory      complement(3361..3456)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(3627..4679)
                     /locus_tag="PXB27_002060"
     CDS             complement(3627..4679)
                     /locus_tag="PXB27_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL5346811.1"
                     /translation="MTHSTKTLVAIASIIIILAGIKLSNEIVVPFLLSLFIAIICSPM
                     IKFMTKRKVPLWLAITILLIVFILGFSFLAGIINNTLQEFTNAIPQYKIQLSSRIQVI
                     LELAHRWHIPLTLSQEEIMSKFDPSSIMNLVSKLLMGLSGALSNIFVIFLVVIFMLLE
                     MPVAKYKLALALSPDDDLSNEKEYINQVLEGVIGYLGVKTFVSLLTGGLVWILLEILG
                     VEYAILWAILTFLLNYVPNIGSILAGIPIVIQALVLNGFSIGLGTLIGIVVINMVVGN
                     ALEPKLMGKKLGLSTLVVFLSLLFWGWLLGTVGMLLSVPLTMACKVALESNKATQKYA
                     VLFSDLEEVPKEQKYR"
     gene            complement(4726..5151)
                     /gene="ruvX"
                     /locus_tag="PXB27_002061"
     CDS             complement(4726..5151)
                     /gene="ruvX"
                     /locus_tag="PXB27_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDL5346812.1"
                     /translation="MTETTTKTVIAFDFGTNSIGCAVGQSITGTAQGLTTFKAQDGIP
                     NWEEIGKLLQDWKPDMLVVGLPLNMDGTEQPLTQRARKFANRLNGRFNLPVELQDERL
                     TTVEAKAEIFSRGGYKALKKDKVDSISACLILESWFENH"
     gene            complement(5151..5711)
                     /locus_tag="PXB27_002062"
     CDS             complement(5151..5711)
                     /locus_tag="PXB27_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HDL5346813.1"
                     /translation="MLENLQGKFLIATPEMDDDYFERTVVYICEHNPQGAMGVVINTP
                     TDLSVLELITRMDFQMANQRDYKKDQMVLSGGPVNQDRGFIIHTKTANDFIHTYPITD
                     KITLTTSGDVLDSFGTLASPEKFIVCLGCSTWRGSQLEQEIAQNYWIVSDANEKTLFE
                     TGYLERWAEAYEMMGLSSIPSRAGRA"
     gene            complement(5711..6442)
                     /gene="rsmE"
                     /locus_tag="PXB27_002063"
     CDS             complement(5711..6442)
                     /gene="rsmE"
                     /locus_tag="PXB27_002063"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007524366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDL5346814.1"
                     /translation="MRIPRIYQPQPLAGLQSCVLSEDAANHVGRVLRMKQGEQIILFD
                     GSNHVFHATLQAVEKKQIIAKIDSSELDDRESNLPIHLGQVISRGDRMEFTIQKSVEL
                     GVKTITPLWSERCGVKLNDERQDKKLQQWQKIAIAACEQCGRNEIPEIRPIMKLTDWC
                     KEQDDMLKLNLHPRAKYTIRELPNVPKAGVRLLIGSEGGLSAEEIAMTESEGFTEVLL
                     GKRVLRTETASLAAITALQVCFGDI"
     gene            complement(6502..>6658)
                     /locus_tag="PXB27_002064"
     CDS             complement(6502..>6658)
                     /locus_tag="PXB27_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5346815.1"
                     /translation="INNLAIFPTMGVKGAIINTRELYHNGYRIVCEVKENEISIITIV
                     HCSRLYP"
BASE COUNT         1955 a         1326 c         1356 g         2021 t
ORIGIN      
        1 ttttgggtag tattagcttt ttcctttatc tattttattc ttcgaatggt tacttattta
       61 gtttatcgtc aactgaatga agagcagatt attaaaggag caaaagccca ttcgcatttt
      121 atggaaactt tatatggtat tacaacgtta aaatcattag gattggaaaa taaacgacaa
      181 gaacagtgga tgtcgctcaa tgcggatgct tttaacacat caattaaggt aactaaattt
      241 gatatgctgt tttccggtat tcataccttt atttctacca tcgaacagat tttaattctt
      301 tggattgggg cgacaatgat gattgaaaat gtgatgacat taggaatgtt tgtcgctttt
      361 aatgcctatc gaggctcttt ctcttcacga atggggaatt taattgatat tatttttagc
      421 ctaaaaattc tttcattaca tagagagcgg attgcagaca ttgctttaaa tgaagtcgaa
      481 gatgaaagta aatatcaaga actcagattt gaacataacc aaacagatat agaagttaaa
      541 gacctttgtt tcaaatacga tcctttttct aaaaatgtgc ttgaaaatgt caatttagtg
      601 atcagaagtg gggaaagtgt tgctattacc gcaccatcag gttatggcaa aacaacgtta
      661 ttaaaattaa ttgcaggttt attaaaacca acctcaggtt ctgtgtattt taacggcatt
      721 gatatttatc agttaggatt aagtcaatat cggagccaaa ttgcttgcgt attacaggaa
      781 gataaattct tttctggttc cattttagag aatattgtta gttttgaaag taattatgat
      841 cgagaatggg ctatcgaatg tagcaaactt gcgaatattc acaatgatat tatggcaatg
      901 ccgatgaatt atgaaacctt attaggagaa ttaggaaata atttgtctgg cggacaaaga
      961 taacgattgt ttattgctcg agctttatat aaaaagccta aaattctttt gatggatgag
     1021 gcgactagcc atttagatga agaaaatgag aagcttgtca atcaagctat ttcacgaatg
     1081 aagataacaa ggattattat tgctcataga caatcgacta ttcagtcggc ggatagaatt
     1141 attgccctga atgacaggta aattaattcc tatttttctg ttcttgccga taaagtttat
     1201 tgcacggaat gctgtcgttg ttgccgtcta gctctttcca gccgcattgt tgaaagtagc
     1261 gttttgcctg tgcgtaattg ccgatttggt tgcagcttaa tttaatttgg caatcaatcg
     1321 ttggtggctg gtttgcaata ttttccggtt ttttgaccgc ttgtggtaag gttgaggtgt
     1381 gtgtttttcg ccattggctc gggttaatcg ggaatttgtc ctgccataag ccgattttag
     1441 cttgctgtgc ttgatcttgt gcttcgatat attcccgctt tgtggtgtaa gcccacgcca
     1501 taccgttttc cactaaggct aagttgatgt ttttatcgtt taagtagagg gtggcaagta
     1561 atcgttggta acgatcatag cctgaaacta tggcagtaat gttttgttga aacacgaatt
     1621 gagccagagc ttgtttggct ttgttgccgt aaggctgcgc ttgttcgggg gcatcaatat
     1681 gttgtagcct tatttttagt ggtgttttat tgaccaaaca agtgagggtg tcgccatcgc
     1741 taatgccaac gactttgcat tgtagttggc gttcggcggc atataccgat gagaaaaata
     1801 agctaaaaat cagggcgtaa agatatttca ttattaaaaa agagagggct aataagccct
     1861 cattctatca ttttttattc ggctttacct gttttaaggc gagcgaagaa atcttttgtt
     1921 tcactaataa tcactttgcg taacgcaatc aatgcgatta aattcgggaa tgccattaag
     1981 ccgttcacga tgtctgccag cgtccaaatt gtatcgagtt gtaaaaatgg agcagaggcg
     2041 atgagtgcaa taaacagcaa gcggtataat ttaatgtatt gggtgcgacc tcgagtgaga
     2101 tagacaaaac agcgttcacc gtagtagcac cagcctaaaa tggtggtaaa ggcaaagaag
     2161 actaaaccaa ctgtcacaat tatcgcaccg aatgagctgt ttaaaccttg agtaaaagcg
     2221 gttgtggtga gttctgcgcc ttttacatcg cctgcccaag cccctgaaat cacaattact
     2281 aagccggtca ttgtgcagac gataatagtg tctaagaacg tgccggtcat tgaaattagg
     2341 ccttgacgta ccggctcttt ggtttgggcg gcagcggcgg cgatgggggc agagcctaat
     2401 cctgattcat tcgagaaaat accacgtgca acccctaatt gaatcgcttg catgacggtg
     2461 aatccgactg ctccgcctaa tgccgcttgt gggttgaaag ctgcgtgtac aatagtaata
     2521 attgcttgtg ggactttttc catattcagt agaatgacgg ataaagcggc gatgacatac
     2581 ccaatagcca taaacggtac aacaatggtt gcaattttag aaatccgctg cacgccacct
     2641 aaaataatag cggtaacgat aagggttaaa atgatcgcac tgaagataat cggcacatca
     2701 aaggtgcttt ctaaggcgtg ggtaatgcca ttcacttggg ggaacgtgcc aataccaagc
     2761 aaagcgacca atacgccaaa aaacgcaaag gctttagcaa gccaacgcca tttttttccc
     2821 attcccagtt cgatatagta catcggacca ccagccacaa atccggctct atctttagtt
     2881 cgatatttaa ttgccaataa gccttcggca tatttcgtag ccataccgaa taaggcaatc
     2941 agccacatcc aaaataatgc tccaggcccg cccgaatgaa tagcggttgc cacgccgaca
     3001 atattccccg tgccgatagt agctgctagg gcagtggaaa gggctgcaaa agcggaaata
     3061 tcgcccgatt gccctttgcc tttttccggt ttgaacatat agcgaaaggc ttgccctagt
     3121 tggcttaatt gaataccttt gagtgaaaag gttaaatata accctacgcc tgaaattaaa
     3181 atcaggagtg gcgggcccca aacaaaactg ttgatttgat taagaatagt gtcgatagac
     3241 atttgatgtt cctcgtaaaa actgcttaca aggaaagaaa aggaatgcaa gagacaagcg
     3301 gtcaaaaaac ggaaattttt tgcaaagaat tagcaaaaaa acaccgcttg tatcgtataa
     3361 cttttctccc ctgtcctttt gcctgagcgt ttcacacatt gctgtatttt cgccttcggc
     3421 ggcggatttt aatccgctct ctccaagggt tcgtccagta acagtccccg cagtgttcac
     3481 tgcacctgaa agagtttacc tcgtcggtga aatgttaatt catttctctc cagctacctt
     3541 catccgaaca atctaattta ttttataatg taaaataaaa aagatttgcc aaattctagc
     3601 aactcttttg cttccgtcaa gtgaatttaa cgatattttt gttctttcgg tacttcttct
     3661 aaatcgctaa ataatacggc atatttttgg gtggctttat tggattctaa tgccacttta
     3721 cacgccatcg taagtggaac agaaagtaac attcctacgg tgccaagtaa ccacccccaa
     3781 aatagaagtg agaggaacac tacaagggta gaaagcccga gtttcttgcc cattaattta
     3841 ggttcaagcg cattacccac aaccatatta atcacgacaa tgccgattaa agtgcctaat
     3901 cctatagaaa agccattgag taccaaggct tgaatcacaa ttggaattcc cgccaaaata
     3961 gagccgatat tcgggacgta gttaagcagg aaggttagaa ttgcccataa aatcgcatat
     4021 tctacgccta gaatttctag taagatccaa actaaccctc cggtgagtag gctcacaaag
     4081 gttttaactc caaggtaacc aatcacccct tcaagtactt ggttgatata ctctttttcg
     4141 ttgcttaaat catcatctgg gctaagtgcg agggctaatt tatatttggc aaccggcatt
     4201 tccaatagca taaaaatgac gactaagaaa atcacaaaaa tattcgataa tgcaccggat
     4261 agccccatta gcagtttgct gaccaaattc attattgagc ttgggtcaaa tttgctcata
     4321 atctcttctt gtgagagggt aagtggaata tgccatctat gggcgagttc tagaatcacc
     4381 tggattctag atgaaagctg aattttgtat tgcggaatag cattagtaaa ttcttgcaag
     4441 gtgttattaa taatgcctgc taagaaactg aatcctaaga taaaaacaat aagtaaaatg
     4501 gtaatggcta accataatgg cactttacgc ttagtcataa atttgatcat tggtgaacaa
     4561 ataatggcaa taaatagcga aagtaaaaat ggcacaacaa tttcattgga taatttaatc
     4621 cctgccagaa taatgataat ggaggctatc gccacgagag ttttggtgga atgtgtcata
     4681 atttttcctt ttacaagcgg ttaaaaatca gcaatttttt acaaattagt gattctcaaa
     4741 ccaactttct aaaattaaac aagcggaaat ggaatccact ttatcctttt ttaatgcttt
     4801 gtaaccacca cgagaaaaaa tttccgcttt tgcttcaaca gtagtgaggc gttcatcttg
     4861 tagctcaacc ggcagattaa agcgtccgtt taagcgattg gcaaattttc tggcacgctg
     4921 agttaaaggc tgctctgtgc cgtccatatt caagggtagc ccaacaacca gcatatctgg
     4981 tttccaatct tgcaagagtt ttccgatctc ttcccagttt ggaataccgt cttgggcttt
     5041 aaatgtagtt aaaccttgag ctgtgccggt aatactttgt ccaactgcac agccgatgct
     5101 atttgtgcca aaatcaaagg caatcacggt ttttgttgtg gtttctgtca ttaagctctc
     5161 ccagctcttg acggtatgct tgaaagcccc atcatttcat aggcttctgc ccaacgctct
     5221 aaataaccgg tttcaaacag cgttttttca ttcgcatcag acacaatcca ataattttga
     5281 gcaatttctt gctcaagctg gctgccacgc caagtggaac aacctaaaca cacaataaat
     5341 ttttcaggtg aagccagtgt gccaaaggag tctaacacat caccggaggt agtgagcgta
     5401 attttatcgg tgatcggata agtgtgaata aaatcattgg cggttttggt gtgaataata
     5461 aagccacgat cttgatttac cggcccaccg cttaacacca tttggtcttt tttgtaatca
     5521 cgctggttcg ccatttggaa atccattcgg gtaatcagct ctagaaccga cagatcggta
     5581 ggagtgttaa ttactactcc cattgcacct tgcggattat gttcgcaaat atagaccacc
     5641 gtacgctcga aatagtcatc gtccatttcc ggcgtggcaa ttaaaaattt accttgtaaa
     5701 ttctctaaca ttaaatatct ccaaaacaaa cttgtaacgc agtaattgcc gcaagtgagg
     5761 cggtttctgt gcgtaacact cgtttaccca gtaatacttc agtaaaacct tcgctctcgg
     5821 tcatggcgat ttcttcggca gataatccgc cctcagagcc aatcagtaaa cgaacaccag
     5881 ctttcggcac attcggcagt tcacgaatgg tatatttggc tcttgggtgc aaattcagtt
     5941 tcaacatatc gtcttgttct ttgcaccagt cggtgagttt cataatcggg cgaatttcag
     6001 ggatttcatt tcgtccgcat tgttcacagg cggcaatcgc aattttctgc cattgctgta
     6061 attttttatc ttgccgttca tcattgagtt tcacgccaca acgttccgac caaagtgggg
     6121 taatagtttt tacccccagc tccaccgatt tttgaatggt aaattccatt ctatcgcctc
     6181 gggaaatcac ttgccctaaa tggatcggca ggttggattc tcggtcatct aattcacttg
     6241 aatcaatttt tgcaataatt tgcttttttt cgaccgcttg taacgtagcg tggaaaacgt
     6301 ggtttgaacc atcaaataaa atgatttgct ctccttgttt cattcgtagc actcgcccaa
     6361 cgtggttagc ggcatcttca cttaacacgc aactttgaag accagctaac ggctgtggtt
     6421 ggtaaattct tgggattctc ataattatct agttataaat taaaggcaga cgattgtctg
     6481 ccataggtaa gttaaaacgg gttaaggata aagtcttgaa caatgcacaa tagtaataat
     6541 cgaaatttca ttctctttaa cttcacacac tattctatag ccgttatgat atagttctct
     6601 cgtattaata attgcacctt ttacacccat tgtaggaaaa atggctaaat tattaata
//
LOCUS       DANLUQ010000136         6563 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.137, whole genome shotgun sequence.
ACCESSION   DANLUQ010000136 DANLUQ010000000
VERSION     DANLUQ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6563
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.137"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            95..790
                     /locus_tag="PXB27_002065"
     CDS             95..790
                     /locus_tag="PXB27_002065"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HDL5346816.1"
                     /translation="MARNIIGILGGMGPAATADMFQKFIRLTPADCDQAHIPLLISSI
                     PDIPDRTRYILANGENPAPVMEKYIEGLESAGATCIIIACNTAHYWFEHLKAKSQVEM
                     LSMIDCVVDEVLKCGKWRVGLLATDATLAVDLYKKQLEAQGLTFICPTENGQKAVMDS
                     IYLLKSGELEKATELMLQQRDELIRLGAEVIILGCTEVPIILAQEIEREPMRYVDSTL
                     VLVKKAIDWYQTH"
     gene            806..2116
                     /locus_tag="PXB27_002066"
     CDS             806..2116
                     /locus_tag="PXB27_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HDL5346817.1"
                     /translation="MIYAEIFVVLAAIYLGLRSGGLGIGLYGGLGLAILTLGFGLPMG
                     SIPVDVMLIIMTVVIGAAVLQAVGGMDLLVRYAEVLMRKNPKYINFIAPMITWLMTIM
                     AGTGFIVFSTLPVIAEVAKESGIRPSRALAGSVVSSQLAIAGSPLSAAMAAMIAVMEN
                     NSVTFFQVIAVCLPVSFIASMVAAFVASKQGCELQNDVVYLERLKAGIVSPSRKLASE
                     STYSKGAKLSIGIFLLATVLVVLLAAVPGLKPVYENGKSMSTRDIIIVMMLASSCLMM
                     LVGKIKPDTIVASPIFRSGMTSIAVIIGIVTLGMTFVDAHLIEIKASIGNILTEYPML
                     LAVVLFFTCALLYSQGSTSALIIPLAVSLGIPNWAILASFIAITGIFILPTYPTSLAA
                     MELDTTGSTRAGKYIIDHPFMLPALVGVAVGLAFGFVWAPMIVS"
     gene            2294..3745
                     /locus_tag="PXB27_002067"
     CDS             2294..3745
                     /locus_tag="PXB27_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HDL5346818.1"
                     /translation="MPNHKFLLLRFQAYINGKFVSSQNGKTFTVQNPTNGEKIGEVAD
                     LGTEETAQAIEAAAVAQKKWQALLPKERSVILRKWFELIMQNQEELAQILSLEQGKPL
                     AESRGEIAYGASFIEWFAEESKRIYGDVLPQDKAGRRLFVTKQPIGVVAAITPWNFPN
                     AMITRKAAPALAAGCAIVIKPAAETPLSALALAQLADQAGIPKGVFNIITSSDVEGVG
                     RELSTNPTVRKLTFTGSTRVGKILMQQSAGTVKKLALELGGNAPAIVFDDADLDTAVE
                     GVFASKFRNGGQTCVCANRIYVQAGIYEQFLAKLSEKVAQIKLGSPFEEEVTIGALIS
                     ERAVEKVESHINDALSLGAELVLGGERHALGGTFFQPTILKNVTQAMKVAREETFAPL
                     APIFKFETEEEVIQWANDTEFGLAAYLFSQNIGRIWRVSEQLEYGIVGINEGIISNEI
                     APFGGWKESGLGREGSKYGIEEFLEIKYLCMKV"
     gene            complement(3821..4645)
                     /gene="iolB"
                     /locus_tag="PXB27_002068"
     CDS             complement(3821..4645)
                     /gene="iolB"
                     /locus_tag="PXB27_002068"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HDL5346819.1"
                     /translation="MSSPLLSKPNFNQHEGLIQHITPENAQWEYVGFDVYQLSKQQIT
                     LESGENELCLVFVTGFGSVYVDDYAFENIGNRTTPFEKIPPYAVYIPPHKSVLVKTEL
                     NLELAVCKAPSQGNYPVRLISPNHIGVEKRGFGNNQRLVHNILPETEAADSLLVVEVF
                     TDEGNTSSYPSHKHDDKHSTEETYLEETYYHRFDPPQGFAMQRVYTDDRTLDECMAVY
                     DKNVVKVPRGYHPVATIAGYNNYYLNVMAGPVRKWKFTWEQDHLWVNSETYKAKFE"
     gene            4864..6375
                     /locus_tag="PXB27_002069"
     CDS             4864..6375
                     /locus_tag="PXB27_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HDL5346820.1"
                     /translation="MSQFVQNFINGAVVESQSQRVTSVFNPATGEETKKVKLSLPNEV
                     DAAIAAADQAFKSWSQQSPLRRARILFKFKELLEANFDELARLISSEHGKIYSDAIGE
                     LTRGLEVVEFATGIPHLQKGEFSANAGRGIDIHSIQQPLGVVAGITPFNFPAMVPMWM
                     FPVALACGNTFVLKPSEKDPSVSIRLAELLKEAGLPDGVFNVVQGDKEAVDILLTDSR
                     IQAVSFVGSTAIAQYIYEKGSANGKRVQALGGAKNHALIMPDADVQGTINALLGAAFG
                     AAGERCMALSVAVVVGDELADKIIEELIPKVKALKIGSGLLPQGVLENDMGPVISKEH
                     KAKIESYIDQGVAQGAKLCVDGRGYKVEGFENGYFVGGTLFDNVTPEMTIWQDEIFGP
                     VLSVVRVKDYAEGIALINSHQYGNGSAIFTADGDSARQFTQDVQAGMVGVNIPIPVPM
                     AFHCFGGWKASIFGPLNVYGTDGVRFYTRMKTITTRWPDSSVRSQAAFNFPTL"
BASE COUNT         1826 a         1250 c         1528 g         1959 t
ORIGIN      
        1 tttttgcata ctgattcgat ttattgcatt tcttttttgc ataacttgtt tgtataagtc
       61 atcgcagtaa attaaatctt ataaggagaa gtgtatggca agaaatatta ttggcatttt
      121 aggtggaatg ggacctgcag caacagcaga tatgttccaa aaatttatcc gcttaacacc
      181 agctgattgt gatcaagctc atattccatt attgatttcc tctattcccg acataccaga
      241 tagaactcgc tatattttag caaatggaga gaatccggct ccggtaatgg aaaaatatat
      301 tgaagggctt gagagtgcag gggcaacctg catcattatt gcatgtaata ctgcacatta
      361 ttggtttgaa cacttgaagg ccaaaagtca ggtagagatg ctaagtatga ttgattgtgt
      421 ggttgatgag gttttgaagt gtggaaaatg gcgggttgga ttgttggcaa ctgatgcaac
      481 tttggcggta gacctataca aaaaacagct tgaagcacaa ggactgactt ttatttgccc
      541 gacagaaaat ggtcaaaagg cagtaatgga tagtatctat ttattaaaat caggtgagtt
      601 agagaaagcc acagagctaa tgttacaaca acgtgatgaa ctcattcgtt taggtgcaga
      661 agtaattatt ttaggttgta cagaagtacc tattatttta gctcaagaaa tagagcgtga
      721 gccaatgcgt tatgtagatt caacgttggt attggttaaa aaagcaattg attggtatca
      781 aactcattaa tttaaggaga tggatatgat ttatgcagaa atctttgtgg tacttgctgc
      841 aatctattta ggactgcgtt ctgggggctt aggaatagga ctatatggtg gtttagggct
      901 tgcgattcta actctaggtt ttggtttacc tatgggttct attccagttg atgtaatgct
      961 gattattatg actgttgtaa taggtgctgc tgtgttacaa gcagtaggag gtatggattt
     1021 acttgttcgt tatgcggaag tattgatgcg taaaaatcct aaatatatta actttattgc
     1081 accgatgatt acttggttaa tgacgattat ggctggtaca ggttttatcg tcttttccac
     1141 cttacctgtg attgcagaag tggcaaaaga gagtggtatt cgcccttcaa gagcattagc
     1201 cggctcggtt gtgtcttctc aattagctat tgcaggttct ccattgagtg ctgcaatggc
     1261 ggctatgata gcagtaatgg aaaacaacag tgttacattt tttcaagtaa tagcagtatg
     1321 cttacctgtt tcttttattg cctctatggt tgctgcattt gttgcttcta agcagggctg
     1381 tgaattgcaa aatgatgttg tttatttaga acgtttaaaa gcaggaatcg tcagcccatc
     1441 tcgtaagtta gcgagtgaat ctacctattc aaaaggagca aaactatcga ttggcatttt
     1501 cttactagca actgtattgg ttgttctact tgcagccgtt ccagggttaa agccagtata
     1561 tgaaaatggg aagtcaatga gtacccgaga tataattatt gtgatgatgt tagcctcttc
     1621 gtgcttaatg atgttggtag gaaaaattaa acctgatacg attgtggcta gtccaatttt
     1681 ccgctcagga atgacttcta ttgcggtaat tatcgggatt gttacattag gtatgacttt
     1741 tgtcgatgca catttaatag aaattaaagc ctctattgga aatattttaa ctgaatatcc
     1801 aatgttgtta gctgtagtac tcttttttac ttgtgcatta ctttattcac aagggtcaac
     1861 ttctgctctt attattccat tagcagtaag cttggggatc cctaactggg caattttggc
     1921 ttctttcatt gcaattacag gcatttttat cctgccaacc tacccaactt cattagcagc
     1981 gatggaatta gatactacgg gttcaactcg tgctggcaaa tatattatag atcatccgtt
     2041 tatgcttcca gctttagttg gggttgctgt tgggcttgct tttggattcg tatgggctcc
     2101 aatgattgta tcttaattat taaatatgta gagaatttaa gttaattctc aataataaat
     2161 ggctgcacct tagaagaacg cttgtctaag gtgctttatt attgagtaaa aagttatttt
     2221 tcagctcttt tctctttact gaaattggat tatagtcaat attgttctct ttttaaccat
     2281 tcaaggagtg attatgccaa atcacaaatt tttgctactc cgtttccaag cctatattaa
     2341 cggtaaattt gtttcaagcc aaaatgggaa aacctttacg gtacagaatc cgactaatgg
     2401 tgagaaaatc ggtgaggtgg ctgatttagg cacggaagaa accgctcaag cgattgaagc
     2461 agccgcagtt gcccagaaaa aatggcaggc attattgccc aaagagcgtt ccgtgatttt
     2521 gcgtaaatgg tttgagctga ttatgcaaaa ccaagaagaa ctcgcccaga ttttaagtct
     2581 agaacagggt aaaccattgg cagaaagtcg aggcgagatt gcctatggag ccagttttat
     2641 cgagtggttt gccgaagaaa gcaagcggat ttatggcgat gtcttgccac aagataaagc
     2701 cggtcgccgc ttgtttgtga ctaaacaacc cattggcgtg gtggcagcaa ttacaccgtg
     2761 gaatttccca aatgcaatga ttacccgtaa agcggcaccg gcgttagcgg caggttgtgc
     2821 gattgtgatt aaaccggcgg cagaaacccc gctttctgcc ttagcattag cccaattagc
     2881 ggatcaagca ggtattccca aaggggtatt taacattatt accagtagcg atgtcgaagg
     2941 tgtagggcgg gagttgagca ctaatccgac cgtacgcaaa ctcaccttta ccggctccac
     3001 ccgtgtcggc aaaattttaa tgcaacaatc ggcagggacg gtaaaaaaat tggcattgga
     3061 gctaggcggt aatgcaccgg cgattgtgtt tgatgatgcg gatctagata cagcggttga
     3121 gggcgtgttt gcctctaaat tccgtaacgg tgggcaaacc tgcgtctgtg ctaaccgaat
     3181 ttatgtgcaa gcgggcattt atgagcaatt tcttgcaaaa ttgagtgaga aagtggctca
     3241 aatcaagctt ggctcgccat ttgaggaaga ggtcacgata ggtgcactaa ttagtgaacg
     3301 agcggtggaa aaagtcgaaa gccacattaa cgatgccctt tcacttgggg cagaactggt
     3361 gttaggcggt gaacgccacg cactgggtgg cacattcttc cagccaacca ttctgaaaaa
     3421 tgtcactcaa gcgatgaaag tggctcgtga ggaaaccttt gctccccttg ccccgatttt
     3481 caaatttgaa accgaagaag aggttatcca gtgggcgaat gatactgagt ttggtttggc
     3541 ggcttactta ttcagccaaa atatcgggcg aatttggcga gtatcggagc agttggaata
     3601 tggcattgtc gggattaatg aggggatcat cagtaacgaa atcgctccgt tcggcggctg
     3661 gaaagagtct ggacttgggc gggaaggttc gaagtacggt attgaagagt ttttagaaat
     3721 aaaatatctt tgtatgaaag tataaccgaa tgggacgcca aaagcgtccc atttttaatc
     3781 ccttattttg taaaaaattg gtggaaaaag accgcttgca ttactcaaat ttggctttat
     3841 aggtttcact gttcacccaa agatgatctt gttcccaagt aaatttccat ttgcgtaccg
     3901 gccctgccat aacatttagg tagtagttgt tatagccggc aatggtagca acagggtggt
     3961 agccacgagg cactttaacc acatttttat catacaccgc catacattca tcaagtgtgc
     4021 gatcatcggt ataaacccgt tgcattgcaa aaccttgtgg tggatcgaag cggtggtagt
     4081 aggtctcttc caaataggtt tcttcggtag agtgtttgtc atcgtgcttg tggcttgggt
     4141 aggaactggt attgccttca tcggtaaaga cttccaccac cagcaaactg tcggctgctt
     4201 cggtttccgg cagaatatta tgcaccaagc gttgattgtt gccaaagccc cgtttttcca
     4261 caccgatatg gttcgggcta atcaaacgca ccggataatt accctgactt ggtgctttgc
     4321 acactgccaa ttctaaattg agttcggttt taaccaacac gcttttgtgt ggcgggatat
     4381 agaccgcata aggcggaatt ttttcaaatg gcgtggtacg gttgccgata ttttcaaaag
     4441 cataatcatc aacatagaca gagccaaaac cggtcacaaa gactagacaa agttcatttt
     4501 caccactttc cagcgtaatt tgttgcttac tgagctgata aacatcaaat ccaacatatt
     4561 cccactgtgc attttcaggg gtaatgtgct gaattaaccc ttcatgttgg ttgaaattgg
     4621 gtttagacaa taatggacta gacataggaa acctcgctta acgaaatgga atacgctttc
     4681 aaactatacg aaattttgtc tcattttaat accggcaatc tgtaaacttg ctacttagat
     4741 cacaaaaatg aaataaaatt ttcatttttt agtattatgt attgatattt tccattttta
     4801 gcggtagttt atcggctgta ttttatccgt ttttcctctc aatcagttga taaggagcct
     4861 ccaatgagcc aatttgtcca aaattttatt aacggtgcag tcgttgaaag ccaaagccag
     4921 cgtgttacct cggtatttaa tcccgcaacc ggtgaagaga ccaaaaaagt caaattaagc
     4981 ctgccaaacg aagtcgatgc ggcaattgca gcggccgatc aggctttcaa aagctggtca
     5041 caacaatcac cactacgccg tgccagaatt ttatttaaat tcaaagagtt attggaagca
     5101 aactttgatg agttggctcg tttaatcagc tcagaacacg gcaaaatcta ttccgatgcg
     5161 attggcgaat taacccgtgg tttagaagtg gtggaatttg ccactggtat tccacactta
     5221 caaaaaggtg agttctccgc caatgcaggg cgtggcattg atattcactc aatccagcaa
     5281 ccgttgggcg tagtggcagg cattaccccg ttcaatttcc cggcaatggt accgatgtgg
     5341 atgttcccag tggcacttgc ttgcggtaac actttcgttt taaaaccgtc tgaaaaagac
     5401 ccaagtgtgt ctattcgctt ggcagaatta ttaaaagaag ccggcttgcc ggacggtgta
     5461 ttcaatgtgg tgcaaggtga taaagaagcg gtagatattc ttttaaccga ctctcgcatt
     5521 caggcggtga gttttgtcgg ctcaactgca attgcacaat atatttatga gaaaggctca
     5581 gcaaacggca aacgggttca agccttgggc ggtgcgaaaa accacgcttt gattatgccg
     5641 gatgctgatg tacaaggcac aattaatgcc ttactgggcg cagcatttgg tgcagccggt
     5701 gaacgttgta tggcactatc ggtagcggta gttgttggtg atgaattagc cgataaaatc
     5761 attgaagagc taattcctaa ggtcaaagca ttgaaaatcg gttcaggctt actaccacaa
     5821 ggcgtgcttg aaaatgatat ggggccggtg atttccaaag agcataaagc caaaattgag
     5881 agctatatcg atcaaggggt ggcacagggg gcgaaactct gtgtcgatgg tcgaggctat
     5941 aaagtcgaag ggtttgaaaa cggttacttt gtcggaggta cgctgtttga taacgtgaca
     6001 cctgaaatga ccatctggca agatgaaatc ttcggaccgg ttctctctgt tgtgcgtgtg
     6061 aaagattatg ccgaagggat cgcactcatt aacagtcacc aatacggcaa tggtagtgca
     6121 attttcaccg cagatggcga ttcagcacgt caattcaccc aagatgtaca agccggtatg
     6181 gtgggcgtga atattccaat tcctgtgcca atggcgttcc actgctttgg cggttggaaa
     6241 gcctctattt tcgggcctct caatgtgtac ggcacagacg gcgtgcgttt ctacacccgt
     6301 atgaaaacca tcacaacccg ttggccggac agctcggtgc gtagccaagc ggcatttaat
     6361 ttcccaacct tataataacg agtacatttc ttttttgagt gtttagcggt gagaaatcac
     6421 cgctttttta ttggttttca tctcacaagc ggtcattttt attcaaattt ttgcaaaaaa
     6481 gcgacctgtt ttgaccgctt gcatttatct cttcctaaaa atgtgatcta cgtaacaaat
     6541 ttgaaacaaa gcatttggtt tgg
//
LOCUS       DANLUQ010000137         6417 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.138, whole genome shotgun sequence.
ACCESSION   DANLUQ010000137 DANLUQ010000000
VERSION     DANLUQ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6417
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.138"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..876)
                     /locus_tag="PXB27_002070"
     CDS             complement(<1..876)
                     /locus_tag="PXB27_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HDL5346821.1"
                     /translation="MKWLMLCREPRLYSCCRIKAACEAKGIELDILDQNRMLLGLENG
                     EFKIYYQAGESYDNNRPEPVLLPNYDGILPRFGTGSTEMGCSVLRHFEAKGIPVLNHS
                     AAFALARDKWRTLQKLAKHNLPVPNTNFAGHLVSVKSQLNQFAFPLISKVLNGSQGNG
                     VMLFEGKNNAEAVLATFRQVNEPYLCQQFVGDAKGQDIRAFVIGNEVVAAMSRTSVTG
                     DFRANIHQGGTAQPIELTQMERALAIKATKTIGLDIAGVDFLRTEKGVVILEVNASPG
                     FEGIERVNDVDIASEM"
     gene            complement(873..1592)
                     /gene="nfsA"
                     /locus_tag="PXB27_002071"
     CDS             complement(873..1592)
                     /gene="nfsA"
                     /locus_tag="PXB27_002071"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NADPH nitroreductase"
                     /protein_id="HDL5346822.1"
                     /translation="MQSKPTLETILSHRSIRKFTEQPISEEIFQILIEAGKAASSSNH
                     LQCVTVIRVTDRQIRETLKAVSGMQYVVDCAEFLVFCIDFNKHKQLVPESQLEWAEVS
                     LIGAVDTGILAQNVFLAAESLGLGAVYIGALRNDIERVSKALNLPEYCVPLVGMCLGY
                     PAQEPAIKPRLPNSLVCYNNQYQDFDWTEFAKYNENLTAYYQARIGEPQQWQAAIHKT
                     LDKPVRPHILPFFNQKGLLKK"
     gene            complement(1604..2389)
                     /gene="mtgA"
                     /locus_tag="PXB27_002072"
     CDS             complement(1604..2389)
                     /gene="mtgA"
                     /locus_tag="PXB27_002072"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HDL5346823.1"
                     /translation="MEQSMAKRNKRFLSSLLKKIGLFFIPRKIKSKLGWLWFGSSRLL
                     TFGGTFFLIPILIFSVLPVPYSAYMVQKKVENLVQGKSYPIKKEWVSLNEISWQMQMA
                     VIASEDQKFESHFGIDLQAIETALKLNAKSKKVRGGSTISQQTVKNMFLWHGQSWLRK
                     GIEVPLTLLVENVWSKDRILEVYLNIAEFGNGIFGVEAAAKHFFKKSAKQLTLQESAL
                     LAASLPNPLVFRVDKPGPSMRKRQAWIMRQVSLLDGKNYLDKL"
     gene            complement(2364..2684)
                     /gene="trpR"
                     /locus_tag="PXB27_002073"
     CDS             complement(2364..2684)
                     /gene="trpR"
                     /locus_tag="PXB27_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HDL5346824.1"
                     /translation="MKTLYNQRDPNEWTQFLALLEQAVAEDKLEPFFSMFLTADERGS
                     LGLRLQIVQALLKGEDSQREIQQNLNTSAATITRGSNMLKTLDQDFLQWLNGKVNGTV
                     NGKA"
     gene            complement(2764..3405)
                     /gene="lolA"
                     /locus_tag="PXB27_002074"
     CDS             complement(2764..3405)
                     /gene="lolA"
                     /locus_tag="PXB27_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HDL5346825.1"
                     /translation="MKTLFKKSVFGLALFGLANVALADAQSVAELQRRLEQVSQYSAD
                     FDQTVRSSKGKQIQQGKGKFQVKRPNLFRMDTKSPQENLIVSDGKDLWFYDPFVSQVT
                     VNTVQDAVNNTPFVLLTSGDKSHWDQYDVSQSADTFVLKPKSKKSNLKQFDVRIDQSG
                     LLKGFSTIERDGQSNLYVLRNITTGGVSSDLFKFSVPKGAELDDQRGGKKSKK"
     gene            complement(3516..6374)
                     /gene="polA"
                     /locus_tag="PXB27_002075"
     CDS             complement(3516..6374)
                     /gene="polA"
                     /locus_tag="PXB27_002075"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDL5346826.1"
                     /translation="MATIAKNPLVLVDGSSYLYRAFHAFPPLTNRLGEPTGAMYGVLN
                     MLKSLISQVNPSHIAVVFDAKGKTFRDELFKQYKSHRPPMPDDLRPQIEPLHRIIKAL
                     GIPLISIEGVEADDVIGTLAVQAAKDGKDVLISTGDKDMAQLVNDHIMLINTMNNTLL
                     DREGVIEKYGIPPELIIDFLALMGDSADNIPGVKGVGEKTALALLQGIGSLEQIYANL
                     DQIATLSFRGAKNFAPKLEAEKANADLSYLLATIKTDVELDVTHDQLLTQPQDRAELA
                     QLFEHYEFKRWLNEVNQNANPVTQTSAEKVPNNYQVTQAVRNEENVANVVEIDRSKYE
                     TVDTEQKLSAWIEKLTAARLIAVDTETDSLDAMSANLVGISFALENGEACYIPLGHKQ
                     KAQPQQADMFGDEERSESPSSDELVKNQLNLTACLSKLKPILLNPEIKKIGQNIKYDL
                     TIFANYGIELQGVAFDTMIESYTQNSTGRHNMDDLAARYLGHKTIPFEELAGKGKNQL
                     TFDKIEIAKAAEYAAEDADVTMKLHQVLWEKLQQEPELVKLFDEIEMPLVGVLSRIER
                     NGVLIDPKKLLTQSAEIEKRLEEVETLVHAEAGQTFNLASTKQLQEILFEKLGLPVLK
                     KTPKGAPSTNEEVLDELAQQGHLVPKLLMEHRGLSKLKSTYTDKLPQLINKKTGRVHT
                     SYNQAVTATGRLSSSDPNLQNIPIRNEEGRRIRQAFVAREGYKIVAADYSQIELRIMA
                     HLSNDEGMINAFAEGKDIHRATAAEIFGVELGEVTSEQRRSAKAINFGLIYGMSSFGL
                     SNQLGIGRAEAQKYMDLYFQRYPAVQQFMTDIREVAVEKGYVETLFGRRLYLPDIKSG
                     NAILRKAAERVAINAPMQGTAADIIKVAMIGIDNAIRDNDEIKMIMQVHDELVFEVKA
                     DKIDHYSQLIKTEMEKAISLKVPLIAEVGVGENWDEAH"
BASE COUNT         1741 a         1497 c         1216 g         1963 t
ORIGIN      
        1 catttcagaa gctatatcaa catcatttac tctctctatg ccttcaaatc ctgggctggc
       61 attgacttct aaaataacta cgcctttctc tgtccgcaga aaatcaacgc ctgcaatatc
      121 aagccctatc gttttagttg ctttaattgc taacgcacgt tccatctgag ttaattcaat
      181 cggttgagcc gttccacctt gatgaatatt ggctctaaaa tcgcctgtta cgctggttcg
      241 gctcatggca gcaaccactt cattgccaat cacaaacgcc cgaatatctt gccctttagc
      301 gtcgcccaca aattgttggc atagataagg ctcatttact tgcctaaagg tcgctaacac
      361 ggcctcggca ttatttttgc cttcaaatag cattacgcca ttcccctgtg aaccatttag
      421 cacttttgaa ataagtggaa aagcaaattg attgagttgt gatttcacag aaaccaaatg
      481 ccctgcaaaa ttcgtattag gaaccggcaa attgtgtttg gctaattttt gtaatgttcg
      541 ccatttgtct cgggctaacg caaatgccgc tgaatgattt agaacaggaa tacctttcgc
      601 ctcaaaatgg cgtaacaccg aacagcccat ttcggtactg cctgtgccaa aacggggtaa
      661 aatgccgtca taattcggca acaataccgg ctccggacgg ttgttatcat agctctcgcc
      721 agcttgatag tagatcttaa attcgccatt ttctaacccg agtagcattc tattctggtc
      781 taaaatatcc agctcaatcc cctttgcctc acaagcggct tttattcgac agcagctata
      841 taaacgaggc tctcgacata gcattagcca cttcattttt tcagcaatcc tttctgattg
      901 aaaaacggca aaatatgcgg acgaacaggc ttatccaatg ttttatgaat agctgcttgc
      961 cattgttgtg gctcgccgat tctggcttgg tagtaagcgg tcaaattttc attatatttt
     1021 gcaaattcag tccaatcaaa atcctgatat tgattgttat aacaaaccaa actattcggc
     1081 aaacgaggtt ttattgccgg ctcttgtgcc ggatacccca aacacatacc caccagtgga
     1141 acacaatatt ccggcaagtt aagggcttta gaaacccgct caatatcatt acgcaatgct
     1201 ccaatataca ccgctcccag ccctagtgat tcggcggcta aaaatacatt ctgggccaaa
     1261 ataccggtat ctaccgcccc aatcaacgac acctccgccc attccaactg cgattcaggg
     1321 acaagttgtt tgtgtttatt aaaatcaatg cagaacacca aaaattcagc acaatctact
     1381 acatattgca tacctgaaac ggcttttaat gtttctcgaa tctgccgatc ggttacacga
     1441 atcaccgtca cacattgcag atgatttgaa cttgaagccg ctttacctgc ctcaattaag
     1501 atttggaaaa tctcttcaga aataggctgt tcggtaaatt tacgaataga acggtgagat
     1561 aaaatcgtct caagagtcgg tttactttgc atatttttct cgtctaaagt ttatctaaat
     1621 aatttttgcc atcaagcaaa cttacttgtc gcataatcca cgcttggcgt tttcgcatac
     1681 tcgggccggg tttatctacc ctaaacacca acgggttagg caaagaggct gccaatagtg
     1741 cggattcttg caaggttagc tgtttggctg attttttgaa aaaatgcttt gcggctgctt
     1801 ccaccccgaa aatgccgtta ccaaattccg caatattgag gtacacctct aaaatccgat
     1861 ccttactcca cacattttcg actaacaggg ttaaaggcac ttcaatccct ttgcgtaacc
     1921 agctttgccc gtgccacaaa aacatatttt ttaccgtttg ttgcgaaatg gttgaacctc
     1981 cacgcacttt tttggattta gcattcagtt tcaaggcagt ttctatcgct tgtaagtcaa
     2041 tgccaaaatg gctttcaaac ttttgatctt ctgaggcaat cacagccatt tgcatttgcc
     2101 acgaaatctc attcaggctc acccattctt ttttgatcgg gtaagatttc ccctgaacca
     2161 gattctccac ctttttttgc accatatacg cagaataagg caccggtaac accgaaaaaa
     2221 tcagtatcgg aatcaggaaa aatgtgccac caaaggttaa taagcgacta gaaccaaacc
     2281 aaagccagcc cagtttactt tttattttgc gaggaataaa aaaaagccct attttcttca
     2341 aaagagagct taaaaatcgc ttgttacgct ttgccattga ctgttccatt aaccttgccg
     2401 ttcagccatt gcagaaaatc ttggtcgagt gttttaagca tatttgaacc acgagtgatg
     2461 gttgccgcac tggtatttaa attttgttgg atttcccgtt gcgaatcttc accttttagc
     2521 aaagcttgca caatttgtaa acgcaagcct aatgaacctc gctcatcggc ggtgagaaac
     2581 attgagaaaa acggctcaag tttatcttcc gcaaccgctt gttcaagcag agccaaaaac
     2641 tgcgtccact cgtttggatc tcgctggtta tagagtgttt tcatactgtc ccttgaagta
     2701 aaaacggtga caagcggtca gatttttaca aaattttgca gaaaaacgac cgcttgctct
     2761 ccattatttt ttcgattttt taccgccacg ttggtcgtct aactctgccc ctttcggtac
     2821 gctgaattta aacaaatcgc ttgagacacc gccggtagta atattgcgta acacatataa
     2881 gttactttgc ccgtcacgct caatggtact gaaacctttt aataaaccgc tttggtcaat
     2941 ccgcacatca aactgtttta aattgctttt tttcgatttt ggcttaagca cgaaagtatc
     3001 agcactctgg ctgacatcat attgatccca gtggctttta tcaccgctgg ttaataagac
     3061 gaatggcgta ttattaactg catcttgcac ggtatttacc gtaacttgtg aaacgaaagg
     3121 atcgtaaaac cataaatctt tgccgtcaga tacaatcaaa ttttcttgtg gtgatttggt
     3181 atccattcta aataagttcg ggcgttttac ttggaatttg cctttgcctt gctgaatttg
     3241 tttacctttg ctagaacgca cggtttggtc gaaatccgca ctatattggc ttacttgttc
     3301 caaacggcgt tgtaattctg caacagattg agcatcagcc aatgccacat ttgccaagcc
     3361 aaataacgct aaaccgaata ccgatttttt aaataatgtt ttcatttgtt ttccttttta
     3421 ccaacaagaa ttttcttgtt tgacaataaa atcatcaaaa gtttcatatt aatgaactgt
     3481 tacaacaata caataaaagt cgtgaattgt atagtttaat gagcttcgtc ccagttttcg
     3541 cctacgccaa cttcggcaat cagtggcact ttgaggctaa tcgctttttc catttcggtt
     3601 ttgattaatt ggctgtaatg gtcgatttta tcggctttga cttcaaacac caattcatcg
     3661 tgaacctgca taatcatttt gatttcatca ttatcacgaa tcgcattatc aataccaatc
     3721 atcgccacct taataatatc ggcagccgtg ccttgcattg gggcgttgat tgccacccgt
     3781 tctgctgctt tacgcaaaat agcattgccg gatttaatat caggcaagta caaacggcga
     3841 ccaaataggg tttcaacata gcctttttca actgcaactt cacgaatatc ggtcataaat
     3901 tgttgaaccg ccgggtaacg ttggaagtag agatccatat atttttgagc ctctgcacgc
     3961 ccaatcccta attggtttga aagcccaaat gacgacattc cgtaaatcag cccgaagtta
     4021 attgctttcg cactgcggcg ttgttcggag gtgacttcac ctaactccac cccgaaaatt
     4081 tcggcagcgg tagcacggtg aatatctttg ccctccgcaa aggcgttaat cattccctca
     4141 tcattcgata aatgagccat aatccgtagc tcaatttgtg aatagtcggc agcgacaatt
     4201 ttataacctt ctctcgcgac aaaagcttgg cgaatccgtc tgccctcttc attgcggatc
     4261 ggaatgttct gcaaattcgg atcgctggaa gaaaggcgtc cggttgcggt gactgcttga
     4321 ttgtaagagg tatgcacccg ccctgttttt ttgttaatca gttgtggtaa tttatcggtg
     4381 taggtagatt tcaacttact taacccacga tgctccatta ataacttcgg cactaaatgc
     4441 ccttgttgag ccaactcgtc taacacttct tcgttggttg agggtgcacc ttttggagtt
     4501 ttcttcaaca ccggcaagcc taatttttca aacaaaatct cttgtaattg tttagttgag
     4561 gcaagattga aggtttgccc tgcttctgcg tgtactaatg tttcaacttc ctccaaacgt
     4621 ttttcaattt cagccgattg ggtcaataat tttttcgggt caatcaacac gccattgcgt
     4681 tcaattcgag aaagtacgcc cacaagtggc atttcaattt catcgaataa cttcaccaat
     4741 tcaggctctt gctgtagttt ttcccacaat acttggtgga gtttcatcgt cacatcggca
     4801 tcttctgccg catattcagc cgcttttgct atctcgattt tatcgaaggt cagttgattt
     4861 ttccccttac cagcaagctc ttcaaacgga atcgttttat gccccaaata acgggcggct
     4921 aaatcgtcca tattatgacg accggtgctg ttttgggtgt aagattcaat catcgtatca
     4981 aaagcaacac cttgtagctc aatgccgtag ttcgcaaaaa tggttaaatc atatttaata
     5041 ttctgcccga tctttttaat ctccgggtta agcaaaatcg gcttgagttt acttaaacaa
     5101 gcggtcaaat ttagctgatt ttttaccaat tcatcggaag acgggctttc tgaacgctcc
     5161 tcatcaccga acatatcggc ttgttgcggt tgggcttttt gcttatgccc aagcggaata
     5221 tagcacgcct cgccattttc cagtgcaaag gaaataccga ccaaattagc cgacattgcg
     5281 tcaaggctgt cggtttcggt atccaccgca atcaaacgag ctgcggtgag tttttcgatc
     5341 caggctgata atttctgttc agtatcaacg gtctcatatt tgctacgatc gatctcgacc
     5401 acatttgcaa cattttcctc atttctgacc gcttgtgtta cttggtaatt attcggtact
     5461 ttttcagccg aagtttgagt gaccggattg gcattttgat tcacttcgtt cagccagcgt
     5521 ttaaattcat aatgctcaaa taattgagca agctcagcac ggtcttgcgg ctgtgttaaa
     5581 agttgatcgt gagtaacatc aagttccaca tcggttttga tggtagcaag caaataggat
     5641 aaatcggcat tcgctttttc cgcttccaat tttggggcga aattttttgc cccacggaaa
     5701 gaaagggtgg caatttgatc caaattagca taaatctgtt caagtgaacc aatgccttgc
     5761 aataaagcta atgcggtctt ttcccccaca ccttttacgc ccggaatgtt atcggcggaa
     5821 tcgcccatta atgccagaaa gtcgataatc aactcaggcg gaatgccata tttttcaatc
     5881 acgccttcac gatctaacaa agtattgttc atcgtgttaa tcaacataat gtgatcgttc
     5941 accaattgtg ccatatcttt atcgccggtg ctgatcaaca catctttgcc gtctttagcg
     6001 gcttgcacag caagcgtgcc gatcacatca tcagcttcca caccttcaat cgaaatcagt
     6061 ggaatgccta aggctttgat aatgcgatgc aaaggctcaa tttgagggcg taaatcatcg
     6121 ggcattggtg ggcggtgcga tttatattgc ttaaacaact catcacggaa ggttttccct
     6181 ttggcatcaa acaccacagc aatatggctt ggattcactt gtgaaatcag gctttttaac
     6241 atattcagca cgccatacat tgcccctgtc ggttcgccta agcgattagt gagcggaggg
     6301 aaagcgtgaa atgctcgata taaataggaa gaaccatcca ccaataccag tggatttttt
     6361 gcgatagttg ccataaaaat ctctttataa tcaaataaat attggcgtta ttataca
//
LOCUS       DANLUQ010000138         6377 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.139, whole genome shotgun sequence.
ACCESSION   DANLUQ010000138 DANLUQ010000000
VERSION     DANLUQ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6377
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.139"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..907)
                     /locus_tag="PXB27_002076"
     CDS             complement(<1..907)
                     /locus_tag="PXB27_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL5346827.1"
                     /translation="MFSDNKALHLGKIYHLIEQFELISRTDLAKLSGLAPASVTNLTK
                     ILIDNHFILERTVQTTLSRGRPSVGLAVSNFFWQLLCVTVSSHKIEISLCKLNGAEIY
                     KQDYTISAENYPRLNDFILNFITNFRQLYCVNTSQLLAVSISVIGKINAEKDSITQLG
                     NKNIFCNITSKLSPLFHCPILLSEHFQLWLLAESTVGSLINQDNAIFLQLDDTVNLSV
                     LLSGSLLSKQSKMNVDNMLMPRFSHLSDEIFPELDEIHRYKLVNQVTFPAIVRLIDRY
                     LPNNLKSQEQKISWLCQKIDENQPLA"
     gene            1041..2159
                     /gene="rnd"
                     /locus_tag="PXB27_002077"
     CDS             1041..2159
                     /gene="rnd"
                     /locus_tag="PXB27_002077"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HDL5346828.1"
                     /translation="MESLIHYQWVETNEQLEAVCEQARKTKVVALDTEFIRTRTYYPI
                     LGLIQLFDGQQVSLIDPNTISDFSPFVSLLADKNIIKVLHACSEDLEVFEYQFKQLPE
                     PMLDTQVMAAFAGIGISMGFAKLVSHYLNIELDKGASRTDWLARPLSAEQLQYAAADV
                     WYLLPVYEKLAQALTKSTWQSAVAEECVALLKKAKQPINKEKAYKNIANAWRLNREEL
                     AILQVLAKWRIEEAQKRNLALNFVVKEASLVQIAKSCPKHTSSLLEFMHPNEVRIHGK
                     KILWLVEQGKAIESNDYPELIQRLVDEKGYKYYMQAMLQKLLEIQPLDIVPELVASKR
                     QLNQLFKWYIDGKPSEKIPELLIGWRKPFGEQLLSVLS"
     gene            2216..2698
                     /gene="lrp"
                     /locus_tag="PXB27_002078"
     CDS             2216..2698
                     /gene="lrp"
                     /locus_tag="PXB27_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005695827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HDL5346829.1"
                     /translation="MENKKPPKALDAIDLKILNELQRNGKISNIELSKRVGLSPTPCL
                     ERVKRLEKNNVIMGYKALLNPELLDAPLLVIVEITLVRGKPDVFEEFNKAIQQLDEIQ
                     ECHLVSGDFDYLLKTRVADMVSYRKLLGTTLLRLPGVNDTRTYVVMEEVKQTNYLLLK
                     "
     gene            2709..5369
                     /locus_tag="PXB27_002079"
     CDS             2709..5369
                     /locus_tag="PXB27_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HDL5346830.1"
                     /translation="MIEQLKPHLKGKENLIKLMLLLTGFLGVYLLIAWASYSPLDNAW
                     SVASSITQTTLNKTGHFGAWSIDMLYAMFGKVAVLVPIALIIFSIYSLGMGLSVDMKW
                     KTVFIRLASFLLLMVGLAGLFSVLFSNSAYYLSGGFVGGVWETILSDTIGQFGALLIA
                     MLFTIAGLYFCSVQSLLPILTQFYEWIMAKSEESKTAESLQHLQNTEQKQPLVTENEA
                     VEVVDDHKPQFTDISAFKRPNISGLRHSENVEIDNREMYEIERDLELPTINILIQPDM
                     VNQVAEQATSGYELPNEESNVLQMPTIRLNTAIENEVDTIENSSSLEDLKIAESMLPQ
                     IQLDNDIEDTEEAIDDVREITPEFMVTPPKAMKDVSEQPAYPKGYGDTLIHPLLQRKV
                     ITEKPTTPLPTLDLLDKAPIQSQQITEQEIRETSVRLEAELANFGVKATVEDVLVGPV
                     VTRYEIQPAAGVKASKITNLASDIARGLMFKAIRITEVIPNKPYMGIETPNKHRETVW
                     LRDVLDSDEFRNTTATLPMALGKDISGEPVVVDMAKMPHLLVAGQTGGGKSVGVNTMI
                     LSLLFKLTPEQVRFIMIDPKVVELSIYNDIPHLLTPVVTDMKKAANALRWAVEEMERR
                     YLLVSHLQVRNIEGYNAKIDQAAAMNLPIPDPTWRPGDSMDSLPPPLQKLSYIVLIVD
                     EFADLMMSAGKEVEEYIMRIAQKARAVGIHLILATQRPSTDVITGVIKANIPSRIAFT
                     VASQIDSRTILDSGGAEALLGRGDMLYSGAGSPDIIRIHGAFMKDEEVQRVADNWRAR
                     GKPNYLESIVESRSEEADGKNDGGTGDLDPLFDEVVEYITETGSVSISNIQRRFSLGF
                     NRAARIVDQMEAQGIVSEPLKGGKREVLAR"
     gene            5521..6360
                     /locus_tag="PXB27_002080"
     CDS             5521..6360
                     /locus_tag="PXB27_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HDL5346831.1"
                     /translation="MRLDDIKESKNVEDRRFNNVGRTKVGRGKGGILTFIIVLVGAYY
                     GVDLTGLMGGGVEYEEQTSALNESEEKQLRTLSAKVLTTTENIWTSYFQQNGLTYKEP
                     SLVLYRGAIQTACGTGQSAMGPFYCPVDQKVYLDLSFYDDMRKKLRASGDFAFSYVIA
                     HEVGHHVQNLLGITSKTQRAQMKASSKAEANQISVNVELQADCFAGVWGHQLAKANRL
                     EEGDVEEAFNAAQAVGDDRLQQQSRGYVVPDSFTHGSSAQRLAWFRKGLTTGNPAVCN
                     TFN"
BASE COUNT         2046 a         1116 c         1381 g         1834 t
ORIGIN      
        1 atgcaagcgg ttgattttca tcaatttttt gacaaagcca gctgattttc tgttcctgac
       61 tttttaaatt attaggcaaa taacgatcaa tgagtctcac aatagctggg aacgtcacct
      121 gattgactaa tttatagcga tgaatttcat ctagttcagg gaatatttca tcactgagat
      181 ggctaaatct aggcataagc atattatcaa cattcatctt tgattgttta gacagtaaac
      241 taccactaag caatacactt agattcacag tatcatctaa ttgtagaaaa atagcattat
      301 cttggttaat taatgagcca accgtagatt ctgcaagcaa ccacagctga aaatgttcac
      361 ttaataaaat aggacaatga aacagcggcg agagtttaga tgtaatgtta cagaatatat
      421 ttttgtttcc caattgagta atactgtctt tttctgcatt aattttaccg atcacactaa
      481 tagatacagc caggagctga ctagtattaa cacaatatag ttggcggaaa tttgtaataa
      541 agttaagaat aaaatcattt agcctaggat aattttctgc tgagattgta taatcttgct
      601 tataaatttc tgcaccattg agtttacaaa gtgaaatttc aattttatga gatgaaacag
      661 taacacaaag tagttgccaa aaaaaattag aaacagctaa tccaacggag gggcgacctc
      721 tggataaagt agtctgaacc gtacgttcta aaatgaagtg gttatctatt agtattttag
      781 tcagatttgt tactgaagct ggggctaacc ccgatagttt agctaaatct gttcttgaaa
      841 tgagttcaaa ttgctcaatt aaatggtata ttttacccaa atgaagggct ttgttgtcag
      901 aaaacattta aaatccttat gttagaattt ttagatgaca attatttttt gatatgaaaa
      961 aaataacaat ctttttttgt ttataatgag agacagatca cataattatt ttttacgttt
     1021 aaatatttca ggaaaagtat atggagtctt tgattcacta ccaatgggtt gaaacaaatg
     1081 agcaattaga ggccgtatgt gaacaggcca gaaaaacaaa agtagttgcc ttagatacag
     1141 agtttatccg tacacgtacc tattatccga tattgggttt aattcaactc tttgatggcc
     1201 aacaggttag ccttattgat ccaaatacga tttctgactt ctcgcctttt gtgtcattat
     1261 tagccgataa aaatatcata aaagtattac atgcctgtag tgaggattta gaagtttttg
     1321 agtatcaatt taagcaattg ccagagccga tgttggatac tcaggtgatg gcagcctttg
     1381 ccggcattgg tatttcgatg gggtttgcaa aattggtttc ccactactta aatattgaac
     1441 tagacaaagg cgcttcccga accgattggc ttgccagacc gctaagcgct gaacaattac
     1501 aatatgcggc agctgatgtt tggtatttat tgccagttta tgagaaattg gcacaagcat
     1561 taacaaaaag cacttggcaa agtgctgtag cagaggagtg tgtcgctttg ttgaagaaag
     1621 cgaaacagcc tatcaataag gaaaaagcct ataaaaatat tgccaatgct tggcgattga
     1681 atcgggaaga gttagccata ttacaagtat tagcgaaatg gcggattgaa gaagcacaaa
     1741 agcgcaatct tgccttaaat tttgtcgtga aggaggcaag tttagttcaa atcgcaaaaa
     1801 gctgtcctaa acacacttct tcgctgttag aatttatgca cccaaatgaa gtcagaattc
     1861 acggaaaaaa aatactatgg ttggttgagc aaggaaaagc aatagaatcg aatgattatc
     1921 ctgaactcat tcagcgcttg gtggatgaaa agggttataa atattatatg caagcgatgt
     1981 tgcaaaaatt attagaaatt caaccgcttg atatcgtacc ggagttggtt gcaagtaaac
     2041 gtcaattaaa tcagctattt aaatggtata ttgatggaaa accatcagaa aaaattcctg
     2101 aattattaat cggttggcga aaaccttttg gtgaacagct gctatcggtt ttatcttaaa
     2161 tttcattcgg gttacataga ctatgtacag tataattgcc taaaaaggag tttatatgga
     2221 aaataaaaaa ccaccaaaag cattagatgc gattgattta aaaatcttaa acgagctaca
     2281 acgtaacggg aaaatttcta atattgaatt atcaaagcga gttgggcttt ctccaacgcc
     2341 ctgcttagaa cgggtaaaac ggctagaaaa aaataacgta attatgggct ataaagcttt
     2401 acttaaccct gaattacttg atgccccttt actggtgatt gttgaaatta ctttggtgcg
     2461 tggtaagcct gatgtatttg aagaatttaa taaagcgatt caacaacttg atgaaattca
     2521 agaatgccac ctggtatcgg gggattttga ttatctgtta aaaacccgtg tggcggatat
     2581 ggtatcgtac cgtaagttat taggcacaac attgcttcgt ttgcccggcg taaatgacac
     2641 tcgaacctat gtggtgatgg aagaagtaaa acaaaccaac tatttgttgt taaagtaagg
     2701 agaattgggt gattgaacaa cttaaaccac acctaaaagg caaagagaac ttgatcaaac
     2761 ttatgctttt gttgaccggc tttttgggcg tttatttgct aattgcttgg gcaagttata
     2821 gtccattaga taatgcttgg tctgttgcaa gcagcattac acaaacaacc ttgaataaaa
     2881 caggtcactt tggtgcttgg agtattgata tgctctatgc aatgtttggt aaggttgcag
     2941 ttttagtacc tattgcatta attattttct caatttatag cttgggaatg ggtttaagtg
     3001 tcgatatgaa atggaaaact gtcttcattc gcctagccag tttcttatta ttgatggtcg
     3061 gtcttgccgg gttatttagc gtgctttttt caaactcagc ttattatctg tcgggtggct
     3121 ttgtcggtgg agtttgggag acaatattat ctgatactat cggacaattt ggtgccttat
     3181 tgattgcaat gctttttact atagctggct tatatttttg ctctgttcaa tcattattgc
     3241 caatattaac gcaattttat gagtggataa tggcaaaatc agaagagagc aaaacagctg
     3301 aatctcttca gcatttgcaa aatactgagc aaaaacaacc gcttgtaact gaaaatgaag
     3361 cggtggaagt tgtagatgac cataaaccgc aatttaccga tatctccgcc tttaaacgcc
     3421 caaacattag tggtttaaga cactcggaaa atgtagagat agataaccga gaaatgtatg
     3481 aaattgaaag agatcttgag ctaccgacta ttaatatttt aatacagcct gatatggtta
     3541 accaagttgc ggaacaagct acatcgggct atgagttacc aaacgaagaa tccaatgtat
     3601 tgcaaatgcc gaccattcgc ttaaataccg ctatcgaaaa cgaggtggat acaatcgaaa
     3661 acagttcatc attggaagac ctgaaaatag cagaatctat gttacctcaa attcagcttg
     3721 ataatgatat tgaagatact gaagaagcta ttgacgatgt gcgtgaaatt acaccggaat
     3781 ttatggtaac gccgcccaag gcgatgaaag atgtgtctga gcagccagcc tatccaaaag
     3841 gctatggtga tacgttaatt cacccgttgt tacaacgaaa agtgataacc gaaaaaccga
     3901 caacaccatt gccaacgcta gatttgttgg ataaagcacc gattcaaagc cagcaaatta
     3961 ccgaacagga aatacgagaa acctctgtgc ggctagaagc agaattagca aactttggcg
     4021 taaaagctac cgttgaagat gtattggtcg gtccggtagt gacccgttat gaaatccaac
     4081 cggctgccgg ggtaaaagcc tccaaaatta ccaatttagc gagcgatata gctcgtggct
     4141 taatgtttaa agcgattagg attactgaag ttatcccaaa caaaccttat atgggtattg
     4201 aaactccgaa taaacaccgt gaaacagtgt ggttgcgtga tgtgctagac agcgatgaat
     4261 tccgcaatac cactgcaaca ttgccaatgg cactaggtaa agacatcagt ggtgagcctg
     4321 tggtggtcga tatggcgaaa atgccacact tactggtggc agggcagacc ggtgggggta
     4381 aatcggttgg ggtgaatacg atgattttaa gtctattgtt caaacttaca ccagaacaag
     4441 tccgctttat tatgattgac cctaaagtgg tggagctttc catttataac gatattcctc
     4501 acttattaac gccggtagta accgatatga aaaaagccgc caacgcacta cgctgggcgg
     4561 tggaggaaat ggaacgccgt tatttgctgg taagccattt acaggtgcgt aatattgaag
     4621 gctataatgc gaaaatcgac caagcggcag cgatgaattt gccgattcca gatccaacgt
     4681 ggcgaccggg cgattcaatg gatagccttc cgcctccgct acaaaaatta agctatattg
     4741 tgctgattgt cgatgagttt gcggatttaa tgatgtctgc cggtaaagaa gtggaagagt
     4801 acattatgcg aattgctcaa aaagcccgag cggtgggaat tcacttaatt cttgctaccc
     4861 aacgtccgtc aaccgatgtg attaccggcg tgattaaagc caatattcca agccggattg
     4921 cgtttacagt agcgagccaa attgactcaa gaactatttt agattcaggt ggagcagagg
     4981 cattattagg gcgaggcgat atgctctatt caggagctgg cagcccggat attattcgga
     5041 tacacggtgc gtttatgaaa gacgaagaag tgcaacgcgt ggcggataat tggcgggcaa
     5101 gaggaaaacc aaactacttg gaaagcattg tggaatcccg ctcggaagag gctgatggca
     5161 aaaacgatgg cggaacgggc gatttagatc cgctatttga cgaggttgtt gaatatatca
     5221 cggaaaccgg ttcggtctct atcagtaata ttcaacgccg cttctcgctt ggttttaacc
     5281 gtgcggctag aattgtggat caaatggaag ctcaaggcat tgtttctgag ccacttaaag
     5341 gtggcaaacg agaagtgctg gcgaggtaaa acacatacaa gcggttagat ttctgtgaaa
     5401 ttttgcaaat tttgacaaaa atctaaccga ttgtatttat ctaatgctaa ataagcatag
     5461 tataaggcta gtctttgggc taaaattaac gcaattttta catcttttaa ggttgaatat
     5521 atgcgattag atgatattaa agaaagtaaa aatgttgaag acagaagatt caacaatgtg
     5581 ggtagaacta aggttggtag agggaaaggt ggaatattaa cctttattat cgtgcttgtt
     5641 ggagcttatt atggtgttga cttaacaggt ttaatgggcg gtggcgtaga atatgaggag
     5701 cagacatcag cattaaatga aagtgaagaa aaacagctaa gaaccttatc cgctaaagta
     5761 ttaacgacca cagaaaatat ttggacttcc tacttccagc aaaatggtct aacttataaa
     5821 gagccgtctt tggtgcttta ccgtggggca attcaaactg cctgtggcac cggtcaatct
     5881 gcaatgggtc ctttctattg ccctgtcgat caaaaggttt atttagattt atcattctat
     5941 gatgatatgc gtaaaaaatt aagagcatcg ggcgattttg ccttctctta cgtgattgca
     6001 cacgaggttg gccaccacgt ccaaaattta ttgggcataa caagcaaaac ccaacgggct
     6061 caaatgaaag cctcatctaa agccgaagca aaccaaattt cagtcaatgt agaattgcaa
     6121 gctgactgtt ttgccggcgt ttggggacac caactcgcaa aagccaaccg attagaggaa
     6181 ggcgatgtgg aagaagcctt taatgctgcc caagcagttg gagatgaccg attacagcaa
     6241 caaagtcgtg gctatgtcgt gccggatagc ttcacccacg gctcatctgc acagcgtttg
     6301 gcttggttta gaaaaggttt aaccacagga aatcctgcag tttgtaatac atttaattaa
     6361 aaaataatta aattatt
//
LOCUS       DANLUQ010000139         6249 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.140, whole genome shotgun sequence.
ACCESSION   DANLUQ010000139 DANLUQ010000000
VERSION     DANLUQ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6249
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.140"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            131..1399
                     /locus_tag="PXB27_002081"
     CDS             131..1399
                     /locus_tag="PXB27_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme"
                     /protein_id="HDL5346832.1"
                     /translation="MSDLKQQALDFHEFPIPGKISVTPTKALDTQHDLALAYSPGVAE
                     PCLAIEKDPSASYRYTARGNLVAVISNGTAVLGLGNIGALASKPVMEGKGVLFKKFAG
                     IDVFDIEINETDPEKLVEIIASLEPTFGGINLEDIKAPECFYVEQKLRERMGIPVFHD
                     DQHGTAIITGAAVINGLQVVGKAISEVRLVVNGAGASAIACTNLLRSLGVKKENITMC
                     DSKGVIFQGRGDKMDETKQFYAIEDNGWRTLADAVANADVFLGCSKGGALTQEMVKTM
                     AKDLLILALANPVPEITPAEAKAVRPDAIVCTGRSDFKNQVNNVLCFPFLFRGALDVG
                     AKTINEEMKMAAAHAIAELAKEPVPFEVISTYGELSFGPEYIIPTPFDPRLIVNVSIG
                     VAKAAIQSGVATRPITDWEAYAEQLKKLVA"
     gene            1551..1838
                     /gene="rplY"
                     /locus_tag="PXB27_002082"
     CDS             1551..1838
                     /gene="rplY"
                     /locus_tag="PXB27_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HDL5346833.1"
                     /translation="MSFKFEAEVRSAQGKGASRRLRHNGQVPAIIYGGNAEPVSIILD
                     HDKVNNAQVHDSFYNEVLTIVVAGKEEQVKVQAIQRHPTKPKLVHLDFKRA"
     gene            complement(1997..3037)
                     /locus_tag="PXB27_002083"
     CDS             complement(1997..3037)
                     /locus_tag="PXB27_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL5346834.1"
                     /translation="MARPRKYENNGLPQNLLCRRRKRANGSIVEYYFYVLADGKERSL
                     GTNKHEAILEAAKLNFENSRKSPIVLFIDVAKRYELEVVPTKKAKNTRQSNLQAIHWL
                     CKFFGNPPAPLEKIEPKHISQYLQWRKDTPAVANIEVGLFNTIWNMAREWGYTALPSP
                     SQGVKKFPTKYREVYVEDYILDKIYEFADERMADIIETAYLLGQRPIDICNIHRSHIY
                     DGILHITQQKTGKKVRFEISGRLKEILDKRLGYESDWIFTNKWGKKLKRRSLGDHFKE
                     IRERAMKAYPELADEIAKVQMRDMRAKAATDISLMTTDEQAQKQLGHTSKRMTHHYIR
                     KDKILKPTDEIT"
     gene            complement(3059..3280)
                     /locus_tag="PXB27_002084"
     CDS             complement(3059..3280)
                     /locus_tag="PXB27_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HDL5346835.1"
                     /translation="MNIYTDFLSREEIEFITQCKQKNLIKKQLNIMGIPFKENANGFP
                     VVRRDYAQAKQRKPIATNDSDWVSNAFKA"
     gene            complement(3330..3713)
                     /locus_tag="PXB27_002085"
     CDS             complement(3330..3713)
                     /locus_tag="PXB27_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346836.1"
                     /translation="MHIEDKIAWWLANGETGVSSKTMAFYLGYGIRPKIEGYPHDVSD
                     FRRCFLLLETVPFLRNRIEKMAELGKVWAALAKEWHTLEALYNEEEDQIRCPKTYAKL
                     REILEANEENVVRIGNVSISIGSTT"
     gene            complement(3801..3977)
                     /locus_tag="PXB27_002086"
     CDS             complement(3801..3977)
                     /locus_tag="PXB27_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346837.1"
                     /translation="MTEEDKEYLQTKIENEGFEYAFVSYSDFEEVQDEKFHGLRKAYL
                     KARSELAEYIDIED"
     gene            complement(4231..4932)
                     /locus_tag="PXB27_002087"
     CDS             complement(4231..4932)
                     /locus_tag="PXB27_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346838.1"
                     /translation="MSTQISTQTISFNNQALITFEQNGTHYTAMKPICENIGLTWHAQ
                     YERIKRDEILSQGILIIRIPTNGGDQQMICLPIEYLNGWLFGIDINRCKPEIRETLIK
                     YKKECYQALHDYWFKGKAERSQIAPIVEPKIPMELTKKEWLRFASMWYALYNSLEVLA
                     TLEKPLRAIGSPFGATAYTHATEYQTTLGVMKRILEPMLADFEVDPFEEAHYHKALTT
                     LRQYQPKGLGGLVRI"
     gene            5477..5692
                     /locus_tag="PXB27_002088"
     CDS             5477..5692
                     /locus_tag="PXB27_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346839.1"
                     /translation="MQTEKRGRGRPKSGLTLQELQAKSDAKRGVRLKSFKFHEDFIAQ
                     LEQLAEQHGISQTQVIVQAVEQFSKGA"
     gene            complement(5744..5962)
                     /locus_tag="PXB27_002089"
                     /pseudo
     CDS             complement(5744..5962)
                     /locus_tag="PXB27_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014390712.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
BASE COUNT         1720 a         1167 c         1322 g         2040 t
ORIGIN      
        1 gtattatttt ttgtaaatat cataaaaaat gagagcaaag ccataaaaat gcgaaaaaat
       61 ttagggtaaa atggctactc tttggctatt attgattact tttattttta tagattataa
      121 ggtttctaaa atgagcgatt taaaacaaca ggcgcttgat tttcacgagt tcccaatccc
      181 cggaaaaatt tccgttactc ctactaaagc attagatact cagcacgatt tagctttagc
      241 gtactctcct ggggttgctg agccttgctt agcgattgaa aaagatcctt cagcttctta
      301 tcgttatacg gcacgaggta acttagttgc agtaatttct aatggtacag cagtgttagg
      361 cttaggcaat attggtgctt tagcaagtaa gcctgtgatg gaaggcaaag gtgttctatt
      421 taagaaattt gccggaattg atgtgtttga tattgaaatt aatgaaactg atccggagaa
      481 attagtggag attattgcat cgttagaacc gacttttggt ggtattaatc tggaagatat
      541 taaagcacct gaatgttttt atgtagagca aaaattacgt gaacgtatgg gaatccctgt
      601 gttccacgat gatcaacacg gtacggcaat tattaccggt gcggcggtga ttaacggatt
      661 acaagtggta ggtaaagcaa tttcagaagt acgtttagtg gtaaacggtg ctggagcatc
      721 agcgattgcg tgtactaact tactccgttc attaggtgtg aaaaaagaaa atatcacaat
      781 gtgcgattct aagggcgtta ttttccaagg tcgtggtgat aagatggatg aaaccaaaca
      841 attctatgca atagaagata atggttggag aacattggca gatgccgttg cgaatgcaga
      901 tgttttctta ggttgttcaa aaggcggagc tttaacgcaa gaaatggtta aaacaatggc
      961 aaaagatcta ttaattcttg ctttagcgaa ccctgtgccg gaaattacac ctgctgaagc
     1021 gaaggctgtc cgtccagatg cgattgtatg taccggtcgt tcagacttta aaaaccaagt
     1081 gaataatgtg ctttgtttcc cgttcttatt ccgtggagca ttagatgttg gggcaaaaac
     1141 cattaatgaa gaaatgaaaa tggcagcagc ccatgcgatt gcagagttgg ctaaagaacc
     1201 tgtgccgttt gaagttattt caacttatgg cgaattatcg tttggcccgg aatatattat
     1261 tccaacgcca tttgatccac gtttgatcgt gaatgtctcg attggtgtag caaaagcagc
     1321 aattcaatca ggtgtagcaa ctcgaccaat tacagattgg gaagcttatg cagagcagtt
     1381 gaaaaaactg gttgcttaat atttttacat tgcatttttc tgcaacttca gtaaaatacg
     1441 tagttggcat atcgagacgg atatgccgat attttttcgg aaacagctgg gtcgcctgct
     1501 gttttttcta ttcacgggga tttccccatt ttatttaaga gaataaaact atgtcattca
     1561 aatttgaagc tgaagttcgt tctgcgcaag gtaagggtgc gagccgccgc ctgcgtcaca
     1621 atggtcaagt tcctgcaatc atctacggtg gtaatgcaga gcctgtatca atcattttag
     1681 atcacgataa agtgaacaac gcacaagttc acgattcatt ctataacgaa gtattaacta
     1741 tcgttgttgc aggtaaagaa gagcaagtaa aagtacaagc aattcaacgc cacccaacta
     1801 aaccaaaatt agttcactta gacttcaaac gtgcttaatc cttgatttac aagggtttaa
     1861 cgctgttttc tgttccgaaa ctttttgcga ttttatagtt ttttaggtca ataaaatcaa
     1921 ttatttacaa aatagtttcg gaacggattt taggtgagta aatggctctt ttgtatttgg
     1981 caaaagggct ttatttttag gtgatttcat cggtaggttt gaggattttg tcttttctga
     2041 tgtaatgatg tgtcattctt tttgatgtat gcccaagttg tttttgagct tgttcatcgg
     2101 ttgtcattag ggaaatatcg gtggctgctt tggcacgcat atcacgcatt tgtactttag
     2161 cgatttcatc agcaagttca ggatatgctt tcattgctct ctcccttatt tctttaaaat
     2221 gatcgcctaa acttcgccgt tttaattttt tcccccattt attggtaaaa atccagtctg
     2281 attcgtatcc caacctttta tctaaaattt cttttagccg accactaatt tcaaatctca
     2341 cttttttccc tgtcttctgt tgagtaatat gcaatatacc gtcatagatg tgtgagcggt
     2401 gtatattaca aatatcaatc ggtctttgtc ccagcaaata ggctgtttcg ataatatccg
     2461 ccattctttc atctgcaaat tcatagattt tatcaaggat ataatcctct acataaactt
     2521 cacgatattt ggtcgggaat ttttttacac cttgtgatgg gcttggaaga gcagtgtatc
     2581 cccattctct tgccatattc caaattgtgt taaataaacc tacctcaata ttagcaacag
     2641 ccggcgtgtc ttttctccat tgtaaatatt gactgatgtg ctttggctca attttttcga
     2701 gcggtgcagg tgggttacca aagaatttac atagccaatg aatagcctgt aaattagatt
     2761 gacgagtgtt tttggctttt ttggttggaa caacttccaa ttcatagcgt tttgccacat
     2821 caataaataa aacaattggg gattttctac tattttcaaa atttagcttg gctgcttcta
     2881 aaattgcttc gtgtttgttt gtaccgagag acctttcttt gccgtcggca agaacgtaaa
     2941 agtagtattc aactatactt ccgtttgctc gttttctgcg gcggcagagt aggttttgtg
     3001 gcaatccgtt gttttcatat tttcgagggc gtgccataag tgcctccttc ttctcaattt
     3061 acgctttaaa tgcatttgat acccagtcag aatcattcgt tgcaataggt ttacgctgtt
     3121 tagcctgagc ataatcacgg cggacaacag ggaatccatt tgcattttct ttaaatggaa
     3181 tgcccataat gttgagttgt tttttgatta gatttttctg cttgcattga gtaataaatt
     3241 caatctcttc acgagaaagg aagtcagtgt agatgttcat attctctcca aataaacccc
     3301 tcatttagag ggatttggtt aaataatcgt taagtcgttg aacctatcga aatgctcaca
     3361 ttaccgatgc ggacaacatt ttcttcgttg gcttctagga tttctctcaa cttagcatag
     3421 gttttggggc atcttatttg atcttcctct tcgttgtaga gagcctctaa tgtgtgccat
     3481 tcttttgcaa gtgcagccca aactttccca agttcagcca ttttttcaat cctatttcgc
     3541 aaaaaaggga ctgtctccaa caacagaaag caacgcctga aatccgatac atcgtgtggg
     3601 tagccttcta tttttggtct tattccgtaa ccaaggtaaa aagccattgt tttactgctt
     3661 acccctgtct ctccattggc aagccaccaa gcgattttgt cttctatgtg catttttcct
     3721 ccaaaaatta accgcttgca gttagctaca agcggttggt tgtcaagtgt tcatttgttc
     3781 aatgaacagt tcatcagtgg ttaatcttca atatcaatat attccgctaa ctcacttcga
     3841 gcttttaaat aagccttgcg aagcccgtgg aatttttcat cttggacttc ttcaaagtca
     3901 ctataactga cgaaagcgta ttcaaaacct tcgttttcaa ttttagtttg aagatattct
     3961 ttgtcttctt ctgtcatttc gtttctccta ttgagagtgg ataaaaataa acactgcacg
     4021 ggcagggtcg gggaagcatt atcgctgtgc gatagtgcat ggtttgccat cgcagtcgtt
     4081 gtgtaactct aagtagtcgg cagcaactgc aaaggcggtt aaaaagagta tgatttttgc
     4141 gtatttcatc gcctaatcct tgaattttgg gtgcaggaaa ccgccgcttg atgtttcaca
     4201 agcggtcgga ttgtgtgatt tttttgcaaa ttaaattcgg actaagccgc ctaagccttt
     4261 cggctggtat tgccttaagg tagttagggc tttgtggtag tgagcttctt caaacggatc
     4321 gacctcgaag tcggctaaca tcggttcaag tatccgtttc attacgccaa gcgtggtttg
     4381 atactcggtg gcgtgagtgt aggcggttgc cccaaacggc gaaccgatag cacgcaacgg
     4441 tttttctaac gtagcaagta cttcaaggct gttgtagagg gcgtaccaca ttgaagcgaa
     4501 acgtagccat tcttttttgg ttagctccat tgggatttta ggttcgacaa tcggggcgat
     4561 ttgtgaacgt tccgctttgc ccttgaacca gtaatcgtgc agggcttggt agcactcttt
     4621 tttgtatttg atgagcgttt ctctgatttc gggtttgcag cgattgatgt caatgccgaa
     4681 gagccagccg ttgaggtatt caattgggag gcagatcatt tgttgatcgc caccgtttgt
     4741 aggtattctt atgataagaa taccctgcga aaggatttca tctcgtttga tccgttcgta
     4801 ttgagcgtgc caagtcaagc cgatattttc gcagatcggt ttcattgcgg tgtaatgtgt
     4861 gccgttttgc tcgaatgtga ttaaggcttg gttgttgaaa gaaatggttt gagtagaaat
     4921 ttgagttgac atagtcgtaa tccttagagt ctatttgttt aaaacaagcc actttcgaca
     4981 gtggcgtcgg gaggttcgaa aacccactct aaggtaaggg ctggacgtat ttcctttcgg
     5041 tcttgtatta gtcgccctcc cgacatagtc aggattacgg atataaaaaa atcgcctagt
     5101 ggcgattagt gaactatccg ccttagagta tgaggtttcg acaccttggg cggaatagta
     5161 agttaaagtt tggcggttgt caaataaaat tattttaata aatactaaaa aatagcttgc
     5221 gttttatttg gtatgtatta aaataatctt gttttcggaa agggtctgaa aatgaagaag
     5281 ccgcccttga agagagcggc aaacatcagg aactggatta tgtatcttaa gttgttaatc
     5341 ctagtgatct taatcttaat aagcacgcca gcttattaga ttagaaagct aaaagtccta
     5401 gcagagaacg ccatctctgc taggcagttc ctaaataata taacgttccg tattaaaaat
     5461 caacaaggaa ttgacaatgc aaacagagaa acgaggtcgt ggtcgcccta aatcaggttt
     5521 aacgcttcaa gaattacaag caaaaagcga tgcaaagcgt ggtgtgcgat tgaagtcatt
     5581 caagttccac gaagatttta ttgctcaact tgaacagctt gcagaacaac acggcatttc
     5641 gcaaacacag gtaatcgttc aagcggttga gcagttttca aaaggggcgt aagcccctat
     5701 aaacctaccc ttcactgttc gatgttttta attagttgtt gcatttaatt tggtgtaatt
     5761 ccaccaaatt aaaatttgga ttattcagct cttcccaaac ttgaagaaat tcaatggtat
     5821 ttttattttg aagccagttt ttaatgagct ggtcgctgtc tccaaaagct ttgcacatat
     5881 cagttaggct gatgtaatcc tcaccatcac gagcggttac tttgacctcc acgccttgta
     5941 caatcattac gctatttgcc atttcgtttc tccgttttta ggtacaaaaa aagccgttgg
     6001 tggacggctt gaaagtgcgg ttatcttaat ccgaagtttg gcggtgtcaa gcctcaatat
     6061 ctatttttgc gatctgcatc gaaaaaataa atttttgttc tgacagtaaa acgctttcgt
     6121 agtaatctca taaaaaaata taaggagaac actatgaaag agcttttcaa acaatggcta
     6181 atcaatcagg actcgccatt cattaatagc tgtggcgttg agtgtatttt gtcaaaagtt
     6241 gatgaccgg
//
LOCUS       DANLUQ010000140         6192 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.141, whole genome shotgun sequence.
ACCESSION   DANLUQ010000140 DANLUQ010000000
VERSION     DANLUQ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6192
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.141"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(27..425)
                     /gene="mutT"
                     /locus_tag="PXB27_002090"
     CDS             complement(27..425)
                     /gene="mutT"
                     /locus_tag="PXB27_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HDL5346840.1"
                     /translation="MSKPIIQVSAGIIRNEFGQIYLTQRLEGQDFAQSLEFPGGKVDP
                     NETPEEALKRELEEEIGIQVLSAFPYENFCFEYPTKIIEFFFYLVEEWVNEPYGREGQ
                     EGFWIAQSDLDEGAFPPANAQLIKRLKEHA"
     gene            complement(512..3238)
                     /gene="secA"
                     /locus_tag="PXB27_002091"
     CDS             complement(512..3238)
                     /gene="secA"
                     /locus_tag="PXB27_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDL5346841.1"
                     /translation="MISKLLTSIFGSSNDRTLKRLRKRVAQINKLEPTFEQLSDEALK
                     DKTAEFKQRLADGVSLDSLLPEAFATVREASKRVLGMRPFDVQLIGGMVLTGRNIAEM
                     RTGEGKTLTATLSCYLNALTGKGVHVVTVNDYLARRDAETNRPLFEFLGLTVGVNIPG
                     LPSDAKRAAYQADITYSTNSELGFDYLRDNLAHDKNERFQRELHYALVDEVDSILIDE
                     ARTPLIISGPAEDATQIYQAVDQIIPHLIAQDKEDSEEYTGEGDFTLDLKNKQAHLTE
                     RGMVKVEDFLTQMGLMEEGESLYHPARISLLHHVSAALRAHKLFERNVDYIVKDGEIV
                     IIDEHTGRTMAGRRWSDGLHQAIEAKERVAIQGENQTVASITYQNYFRLYEKLAGMTG
                     TADTEAFEFQQIYGLNTVVIPTNRPVIRDDRTDLMFKSEAEKFAAIITEIRDCIERKQ
                     PVLVGTASVEKSELLSAELTKAGIAHNVLNAKFHAQEAEIVAEAGAPGAVTIATNMAG
                     RGTDIVLGGNWKMEVAKLENPTQEQIDAIKAAWQERYDIVMQAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDPGSSRFYLSLDDALMRIYLNEGKLNMMRKAFTEEGEAMESKLLT
                     KVIASAQAKVEAHNFDGRKHLLQYDDVANEQRKVIYEQRNHLLETDNIASMIESIRQD
                     VFESTINQYIPPQSIEEMWNVPGLEERLRREFGLDLPISHWLETEKDLHEETLRERIL
                     NIAIAEYNAKEEIVGAEVMRNFEKGVMLQNLDELWKEHLASMDYLRKGIHLRGYAQKD
                     PKQEYKKESFAMFTNMLDTLKLNVISILSRIQVRSKEEVEEAQRQQAEMDAASTPVTQ
                     ATDSAAGSREENSAESLSEEELANLNIGRNDPCPCGSGKKYKHCHGNKARYS"
     gene            complement(3297..3617)
                     /locus_tag="PXB27_002092"
     CDS             complement(3297..3617)
                     /locus_tag="PXB27_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2547 family protein"
                     /protein_id="HDL5346842.1"
                     /translation="MSLFLKHFHRTPFWSQLFLGMVAILALPEIQVAYANESEQETVL
                     NQSFLQYAQSANDIEQQSLFIAELIHKIVEIKPQAVHFCEFFANSYRLDRQNAYPIRA
                     GPQT"
     gene            3738..4040
                     /locus_tag="PXB27_002093"
     CDS             3738..4040
                     /locus_tag="PXB27_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HDL5346843.1"
                     /translation="MKNSAPKKINDILQNSSLSRIVEKANFINDLNQKIQKRWPESYR
                     GLYRVVNLVDNQLIVSVKNATVRQGLILQQNMLLKQIQTDLPEITQLEFRVNPSVT"
     gene            4065..4439
                     /locus_tag="PXB27_002094"
     CDS             4065..4439
                     /locus_tag="PXB27_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein NlpE N-terminal
                     domain-containing protein"
                     /protein_id="HDL5346844.1"
                     /translation="MKKIALIGFTTAMLGACSLLPQKSVSGTYAGELPCADCEKIQAQ
                     LTLNADKTYQYDTVYFKDKKEYTYRDKGTYSWEPNKPNVIRLEKASGNLAFQVSEQHV
                     ELCDPNGNTVKNSNNYKLGKVR"
     gene            complement(4615..4704)
                     /locus_tag="PXB27_002095"
     tRNA            complement(4615..4704)
                     /locus_tag="PXB27_002095"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4668..4670),aa:Ser,seq:gga)
     gene            complement(4796..5107)
                     /locus_tag="PXB27_002096"
     CDS             complement(4796..5107)
                     /locus_tag="PXB27_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HDL5346845.1"
                     /translation="MSVEQTIIQKIKTEFQPIFFNVENESYMHSSGRGAESHFKLTLV
                     SDKFEGQRTVARHRAVYACLAEELANGVHALALHLYTQGEWEATGGLVPKSLNCLGVG
                     Q"
     gene            5199..5786
                     /locus_tag="PXB27_002097"
     CDS             5199..5786
                     /locus_tag="PXB27_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346846.1"
                     /translation="MKRSKKTLVLATTFVAALLAGCQSQSNTLTFSTPTPTAVFNTNN
                     QTAAVNVMTQDLRTSREIASYTKNGEVIRLTAVPEVAAMFQQAVQQDLNAKGFQIVQG
                     AANANVVVNIRKFFATVEQGNLRYKADAQVSVKVAVQGSKGNFSKNFNATRGYEGAFG
                     ASNDEIKKVLDQAYSDVVKAIYNDNEIANAIHQFK"
     gene            5958..6131
                     /locus_tag="PXB27_002098"
     CDS             5958..6131
                     /locus_tag="PXB27_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346847.1"
                     /translation="MNNPYHNVSQEYLDYLAKKVKQGKEDIKNGRIYSPEEVRKRVIA
                     AAFRGAENAKKVA"
BASE COUNT         1909 a         1318 c         1131 g         1834 t
ORIGIN      
        1 actttagata cagttctcaa tacaaattat gcgtgttctt ttaagcgttt aattaattgg
       61 gcatttgccg gtgggaaagc tccctcatct aaatccgatt gtgcgatcca aaaaccttct
      121 tgcccttctc ttccgtaagg ttcatttacc cactcttcca ccaaataaaa gaaaaattca
      181 atgatttttg ttggatattc aaagcaaaaa ttttcatacg gaaaagcgct taacacttga
      241 ataccaattt cttcttctaa ttctcgtttt agtgcttctt caggggtttc attgggatcg
      301 actttccccc cgggaaattc aagagactga gcaaaatctt gcccttctaa acgttgggtt
      361 aagtaaattt gcccaaattc gttacgaata atccctgctg agacttgaat aatcggtttt
      421 gacatagttt ccttcactct ttctataaat tcaagcggtc atattaagca gaaaatttgc
      481 aaattcctga ctgaatatga ccgcttgtta attacgaata tctcgctttg ttaccatggc
      541 aatgcttata ttttttgccc gaaccacaag ggcaagggtc atttcgacca atatttaagt
      601 ttgccaactc ttcttcactt aagctctctg cgctattttc ttctctacta ccagcagcag
      661 aatcggtagc ttgtgtaact ggcgtacttg cagcgtccat ttcagcttgc tggcgttgcg
      721 cttcttccac ctcttctttg ctacgcactt gaatacggct taaaatgcta attacattca
      781 attttaaggt atcaagcata ttggtaaaca tcgcaaaaga ttccttttta tactcttgct
      841 tcggatcttt ttgtgcataa ccacgcaagt gaataccttt gcgtaaataa tccattgagg
      901 ctagatgctc tttccaaagc tcatctaagt tttgtagcat tacacctttt tcaaaattac
      961 gcattacctc tgcgcctact atttcttcct tcgcattgta ctctgcaatc gcaatattta
     1021 agatacgttc acgcaaagtc tcttcgtgca agtctttttc agtttcaagc cagtgagaaa
     1081 tcggtaaatc caacccaaat tcacggcgta aacgctcttc taagcccggc acattccaca
     1141 tttcctcgat agattgaggc ggaatatatt ggttaatcgt gctttcaaat acatcttggc
     1201 gaatgctttc gatcattgag gcaatattat ctgtttctaa tagatgattg cgttgttcgt
     1261 aaattacttt acgctgttca ttggcaacat catcatattg taataaatgc ttacgcccat
     1321 caaagttatg cgcttctact ttcgcctgtg cagaggcaat cactttggtt aacagtttag
     1381 actccatcgc ttcaccttct tcggtaaagg ctttacgcat catatttaat ttaccttcat
     1441 tgaggtaaat tctcattagg gcatcatcta aagagagata gaaacgtgat gaccccgggt
     1501 caccttgacg accagaacga cctcgcaact ggttgtcgat acggcgggat tcgtggcgtt
     1561 ctgtaccaat aatatgtaaa ccaccggctt gcattacgat gtcataacgc tcttgccacg
     1621 cagctttaat cgcatcaatt tgttcttgtg tcgggttctc aagcttggct acttccattt
     1681 tccagttacc gccaagcaca atatccgtac cacgaccagc catattggtt gcgatagtaa
     1741 ccgcccccgg agctcccgct tccgctacaa tttcagcctc ttgagcgtgg aatttcgcat
     1801 ttaatacgtt atgagcaata ccggctttgg ttaattctgc cgataataac tctgattttt
     1861 ccaccgatgc tgtgccaacc aaaacaggct gtttacgctc gatacagtct ctaatttcag
     1921 taataatcgc cgcaaatttc tccgcttcgc ttttaaacat taagtctgta cggtcatcac
     1981 ggatcaccgg tctgttggtt ggaatcacca ccgtatttaa gccataaatt tgctggaatt
     2041 caaaggcttc ggtgtctgcc gtaccagtca taccggctaa tttttcgtac aaacggaaat
     2101 agttttggta agtaattgag gcaacagttt ggttttcgcc ctgaatagcc acacgctctt
     2161 tagcttcaat cgcttggtgt aagccgtcag accaacgacg tccagccatt gtacgaccgg
     2221 tgtgctcatc aataatcaca atttcaccgt cttttacaat gtaatctacg ttgcgttcaa
     2281 ataatttatg ggcacgcaat gctgcactca catggtgtaa cagcgaaata cgagcagggt
     2341 gatataaact ttcgccctct tccattaacc ccatttgagt taagaaatcc tctactttca
     2401 ccataccacg ctcggttaaa tgagcctgtt tgtttttcag atctaaggta aaatcgccct
     2461 caccggtgta ctcttcactg tcttctttat cttgagcaat caaatgcgga atgatttgat
     2521 ctaccgcttg ataaatttgg gtcgcatctt ccgcaggccc tgaaataatt aacggcgtac
     2581 gagcttcatc aattaaaata gagtctactt catcaactaa tgcgtagtgt aactcacgtt
     2641 ggaaacgttc gtttttatcg tgagctaagt tatcacgcaa gtaatcaaaa cctaattcgc
     2701 tgttagtcga ataggtaata tctgcttggt atgcagcacg ttttgcatcg gaaggtaacc
     2761 ccggaatatt cactccaacg gttaagccta aaaactcaaa taacggacgg ttggtttccg
     2821 catcacggcg ggcgaggtaa tcattcaccg taacaacatg aacaccttta ccggtcaaag
     2881 cattcaaata acaagataaa gttgcagtta gggttttacc ttccccagta cgcatttctg
     2941 caatattacg ccccgttagc accataccgc cgatcagctg tacatcaaat ggtcgcatac
     3001 ctaatactcg tttgcttgct tctctcactg tcgcaaaagc ttcaggtaat aggctatcta
     3061 gcgatacacc atctgcaagg cgttgcttaa actccgctgt tttatccttt aacgcctcat
     3121 cagataattg ctcgaaggtt ggttcaagct tattaatttg tgccactcgc tttcttaaac
     3181 gttttaaagt gcgatcgttg cttgagccaa aaatagaagt cagtaatttt gaaatcattg
     3241 tgttattcct aaatataaaa atgggttaat caattaatgg taaaattaaa ttatatttag
     3301 gtttgtggcc cggctcgaat ggggtaagca ttttgtctat ccaagcggta ggagtttgca
     3361 aaaaattcgc aaaaatgcac cgcttgtggt ttaatttcca caattttatg gattaactct
     3421 gcaataaaaa gcgattgttg ctcaatatca ttagcggatt gagcatattg aagaaaagat
     3481 tgattaagaa cggtttcctg ttcactttca ttggcgtacg caacctgaat ttcaggtaaa
     3541 gcaagtatcg caaccattcc caaaaagagt tgcgaccaaa aaggcgttcg atgaaagtgt
     3601 tttaaaaata agctcattaa attaagataa atgaaaaaat tgggctaatt atacgctaaa
     3661 gtaaagccga atgagtaaat tattgggtca agtatcgtaa aatcaagcct aaaataagac
     3721 gaaatgtaaa gaaacagatg aaaaattcag cacctaaaaa gattaatgac attttacaga
     3781 actcaagctt aagccgtatt gtcgaaaaag caaacttcat taacgattta aaccaaaaaa
     3841 ttcagaaaag atggccagaa tcttatcgtg ggctttaccg agtagtgaat ttagttgata
     3901 atcagcttat tgtgagcgtt aagaatgcaa ccgtacggca aggtttaatc ttgcaacaaa
     3961 atatgttact aaaacagata caaaccgatt tacctgaaat aacccaactt gaatttagag
     4021 taaatccgag tgttacataa tttaattttg aagaggaaaa tagtatgaaa aaaatcgcac
     4081 tcattggttt taccaccgca atgctagggg cttgctcatt gctaccacaa aaaagcgtta
     4141 gcggtactta tgcaggtgaa ttaccgtgtg ctgactgtga gaaaatccaa gcccagctaa
     4201 ccttaaatgc agataaaacc taccaatatg atacggtata ttttaaagat aaaaaagaat
     4261 acacttaccg agacaaaggc acatacagtt gggaacccaa taagccaaat gtgattcgtc
     4321 tggaaaaagc atcaggcaat cttgccttcc aagtgtctga acaacacgtt gaactttgcg
     4381 acccaaatgg caacacggta aaaaacagca ataactataa actaggtaaa gtacgttaaa
     4441 agcagataaa aaggattctt aggaatcctt tttctttgta atctatcgcc gatcgcataa
     4501 aaaaaccccc aagcaatgtt tgctcgggga taacctaaaa atgaaaaagt attatctact
     4561 ttaacttaac caaggaaata tctatgaaaa cagtaaggaa tctttcagta tgaatggcgg
     4621 tgagaggggg attcgaaccc ccgatacagt ttcccatata cacgctttcc aggcgtgctc
     4681 cttcaaccac tcggacatct caccgaatca acaaaaagat ggcgtaaata tagcaatttt
     4741 acacggctct ttcaagtatt ttcttcacaa aataaaataa atcattttta tccacttatt
     4801 gcccaacacc taagcaattt agcgatttag gtacaagtcc gccagtcgct tcccattcac
     4861 cttgggtata taggtgcaaa gcaagtgcgt gaacgccatt agctaattcc tcagctaagc
     4921 aagcatagac agcacgatga cgtgcaactg ttctctgccc ttcaaattta tctgacacca
     4981 aagttaattt aaaatgcgat tctgctcctc tacctgagct gtgcatatag ctttcgtttt
     5041 cgacattaaa gaaaatcggc tgaaattctg tcttaatttt ttgaataatt gtctgttcta
     5101 ccgacatttt ttgctctcgt tcattgaaaa tcaaaacgaa ataatactat actaagcaga
     5161 aattttctcg aaatttattc aatatagaag gatttactat gaaacgctct aaaaaaacgt
     5221 tagtgttagc cactactttt gttgccgcat tactagccgg ctgccaaagc caatccaaca
     5281 cattaacatt ctctacccca acgccaactg ctgtatttaa cacaaacaat caaacggctg
     5341 cggtgaatgt aatgacacaa gatttacgca cctcgcgtga aatcgcaagc tacactaaaa
     5401 acggagaggt cattcgttta accgcagtgc ctgaagttgc tgcaatgttc caacaagccg
     5461 tccaacaaga cctgaatgca aaaggtttcc aaattgtaca aggtgcagcg aatgccaatg
     5521 tggtagtgaa tattcgtaaa ttctttgcca cagtagaaca aggaaactta cgctacaaag
     5581 ccgatgcaca agtgagtgta aaagtggcag tgcaaggttc taaaggaaac tttagcaaaa
     5641 acttcaacgc tacccgtggt tacgaaggtg catttggtgc aagcaatgat gaaatcaaga
     5701 aagtgttaga ccaagcctat tctgatgtgg tgaaagctat ttacaacgat aatgaaatcg
     5761 ccaatgcaat tcaccagttc aaataattaa ttttttctgg caagcggtca tctttcttcg
     5821 attttttgca aaaaacgtgg aaaaatgacc gcttgttgtt ttctcgtttt gtgatgtggc
     5881 tcgaaaaata agataaatta acttgattaa acgatcagta gcccccacaa tagagactca
     5941 tcgcagggag gaaaaatatg aataatcctt atcataatgt tagccaagaa tatcttgatt
     6001 atcttgctaa aaaagtaaag caaggaaagg aagatattaa aaacggtcgg atttattcac
     6061 ccgaagaggt cagaaaaaga gttattgctg ccgcttttag aggtgctgaa aatgccaaaa
     6121 aagttgcata atatttatta tactgaagag gccttaatag atttagagaa tattgccatc
     6181 tcagttagtg aa
//
LOCUS       DANLUQ010000141         6107 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.142, whole genome shotgun sequence.
ACCESSION   DANLUQ010000141 DANLUQ010000000
VERSION     DANLUQ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 6107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6107
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.142"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..211)
                     /locus_tag="PXB27_002099"
     CDS             complement(<1..211)
                     /locus_tag="PXB27_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346848.1"
                     /translation="MSTTLNEINITELDAGPEIKSLSLNDAVNSFLNDSACPICGKNC
                     QYWQRLKRSLNRKENQGKGDKVLVKN"
     gene            complement(245..1531)
                     /gene="purD"
                     /locus_tag="PXB27_002100"
     CDS             complement(245..1531)
                     /gene="purD"
                     /locus_tag="PXB27_002100"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012771675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDL5346849.1"
                     /translation="MNILIIGNGGREHALAWKVRQSPLADKVFVAPGNAGTAMENGIE
                     NVAISATDVPALVTFAKQNSVGLTIVGPEAPLVVGVVDAFRAHGLTIFGPTQAAAQLE
                     GSKAFTKDFLARHQIPTAEYQNFTEIEPALAYLKQKGAPIVIKADGLAAGKGVIVAMT
                     LQEAEDAVRDMLSGNAFGEAGSRVVIEEFLDGEEASFIVMVDGKNVEPMATSQDHKRV
                     GEGDSGLNTGGMGAYSPAPVVTKAIHQRVMDEIIYPTVQGMASENTPYTGFLYAGLMI
                     MPNGQPKVIEFNCRFGDPETQPIMMRLESDLVELCLKACEGKLDSVQSKWCEQAALGI
                     VLAAAGYPGDYRKGDEIIGIPTSTSNQKVFLAGVEQKEGKLLTNGGRVLCATALGNSV
                     FEAQQQALQLAEQIQWQGKFYRRDIGYRAVARKQAK"
     gene            1765..2139
                     /gene="rpsF"
                     /locus_tag="PXB27_002101"
     CDS             1765..2139
                     /gene="rpsF"
                     /locus_tag="PXB27_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDL5346850.1"
                     /translation="MRHYEIVFMVHPDQSEQVPAMIERYTASVKEAGGQVHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQSVIDELETNFRYNDAVLRNLIVHTKAAVTEASPM
                     VKAKESKVAEAVAEVESEEAGE"
     gene            2114..2488
                     /gene="priB"
                     /locus_tag="PXB27_002102"
     CDS             2114..2488
                     /gene="priB"
                     /locus_tag="PXB27_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HDL5346851.1"
                     /translation="MNQRKQASNLPIDNCLILSGTVSSLVKQSLNPLGVPHYSFWLEH
                     RSIQTEVNLERQAWCKIQVVLAGNQFSYLTQQIKLGESVRIKGFIHTHKDFNGLNQLV
                     LHTEQIEFIDQEKPNGTLFPSS"
     gene            2463..2690
                     /gene="rpsR"
                     /locus_tag="PXB27_002103"
     CDS             2463..2690
                     /gene="rpsR"
                     /locus_tag="PXB27_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDL5346852.1"
                     /translation="MARYFRRRKFCRFTAENVVEIDYKDIATLKNYISESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLALLPYTDNHQ"
     gene            2706..3155
                     /gene="rplI"
                     /locus_tag="PXB27_002104"
     CDS             2706..3155
                     /gene="rplI"
                     /locus_tag="PXB27_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011609589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDL5346853.1"
                     /translation="MQVILLDKVAHLGSVGDQVTVKSGFARNFLIPQGKAVMATAANI
                     AHFEARRAELEAKAAAALATAQERAAKIAALAAVSVSATAGDDGRLFGSVSAKDIAEA
                     LTAAGVGVAKSEVRLGEGPLRTTGEHQVKIHLHPEVNATVAVNVVAE"
     gene            complement(3219..3995)
                     /gene="dnaQ"
                     /locus_tag="PXB27_002105"
     CDS             complement(3219..3995)
                     /gene="dnaQ"
                     /locus_tag="PXB27_002105"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HDL5346854.1"
                     /translation="MTEEKIIRQVVLDTETTGMNMAGGPPQIGHNIIEIGAVEVINRR
                     LTGRTYHVYIKPPREVDEEAIAVHGITNEFLQDKPVFAEIAEEFLDFIKGAELIIHNA
                     PFDVAFMDQEFSYLPNPPAKTAEMCTVTDSLQMARRMYPGKRNNLDALCDRLGIDNSK
                     RVLHGALLDAEILADVFLMMTGGQISLLGEEETEVVSEVIDIEEEFSAIVLNADEAII
                     LKANDEEEESHLALLKLIEKKSKGNCLWTKALTDTAEISH"
     gene            complement(4083..5753)
                     /gene="ettA"
                     /locus_tag="PXB27_002106"
     CDS             complement(4083..5753)
                     /gene="ettA"
                     /locus_tag="PXB27_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HDL5346855.1"
                     /translation="MSTQFVYTMHRVGKVVPPKRHILKDISLSFFPGAKIGVLGLNGA
                     GKSTLLRIMAGVDKEFEGEARPQPGIKIGYLPQEPKLAPQQTVREAVEEAVAEVKNAL
                     TQLDEVYAMYADENADFDKLAAKQAELEAIIQAHDGHNLQNQLERAADALRLPDWDAK
                     IEHLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDYEGTVVAITH
                     DRYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKEKRLAQEQAAESARQKSIEKELEW
                     VRQNPKGRQAKSKARMARFEELNSGEYQKRNETNELFIPPGPRLGDKVIEVQNLTKSY
                     GDRTLIDDLSFAIPKGAIVGIIGANGAGKSTLFRMLSGKEQPDSGSIVLGETVVMASV
                     DQFRDAMDDKKTVWEEVSNGQDILTIGNFEIPSRAYVGRFNFKGVDQQKRVGELSGGE
                     RGRLHLAKLLQRGGNVLLLDEPTNDLDVETLRALENAILEFPGCAMVISHDRWFLDRI
                     ATHILDYGDEGKVTFYEGNFSDYEEWKKKTFGEAATQPHRVKYKRIAK"
BASE COUNT         1607 a         1405 c         1288 g         1807 t
ORIGIN      
        1 gattcttcac aagaacttta tctcccttac cttgattttc ttttctattt aaagatcttt
       61 ttagtctttg ccaatattga cagttttttc cacatatagg gcacgcagag tcatttagaa
      121 aagagtttac tgcatcgttt aatgatagtg atttaatttc ggggcctgca tcgagttcag
      181 tgatattaat ttcattaagt gttgtactca taataaattt ttccttgttt taagagttta
      241 taaattattt cgcttgtttt cttgccactg ctcggtagcc aatatctcgg cgatagaatt
      301 tgccttgcca ttgaatttgt tcggcaagtt gtaaggcttg ttgttgagct tcaaatacac
      361 tgttgccaag tgcggtggca caaagtacac gtccaccgtt ggtgagcagt tttccttctt
      421 tttgctcaac ccctgctaag aaaacttttt gattggaggt agaggtcgga atgcctatga
      481 tttcatcgcc tttacggtaa tcgcccgggt agcctgcggc agctaaaaca atgcctaatg
      541 cggcttgttc acaccatttt gattgcacgc tatccaattt gccttcgcac gcttttaagc
      601 acaattcaac gaggtctgat tccaaacgca tcataatcgg ctgagtttca gggtctccaa
      661 aacggcagtt aaattcaatc actttcggct gaccgtttgg cataatcatt aagccggcat
      721 agagaaaacc ggtgtaaggg gtattttcgc ttgccattcc ttgcacggtc gggtaaataa
      781 tttcgtccat tacacgttgg tggattgcct ttgttaccac cggagcaggg gagtaagccc
      841 ccattccgcc ggtatttaag ccgctatcgc cctcgccaac acgtttgtgg tcttgtgagg
      901 ttgccattgg ttcgacattt ttgccgtcca ccatcacaat aaaactggcc tcttctccgt
      961 ctaaaaattc ctcgatcacc acacggctgc cggcttcacc aaaggcgttg cctgacagca
     1021 tatcacgcac tgcatcttcc gcctcttgta aagtcattgc aacaattacg cctttgcctg
     1081 cggctaaacc gtctgctttg attacaatcg gtgcaccttt ttgttttaag taggcaagtg
     1141 ccggttcaat ttcggtgaaa ttttggtatt ctgcggttgg aatctgatgg cgagccaaga
     1201 aatctttggt aaatgcttta gaaccttcta attgagcagc ggcttgggtc gggccgaaaa
     1261 tggttaaacc gtgggctcga aaagcatcaa ccacgccaac tactaacggt gcttcagggc
     1321 cgacaatcgt caaacctacg ctattttgtt tagcgaatgt aactaaggca ggcacgtcag
     1381 tggcggaaat agctacattt tcaatgccgt tttccatcgc tgtgccggca ttgccggggg
     1441 cgacaaagac tttgtctgcg agcggcgatt gacgaacttt ccaagcaaga gcgtgttcac
     1501 gcccaccgtt gccgataatt aaaatattca ttgggattcc ttgcaaaaat gataaagcaa
     1561 acgtttgcgt attgtacttg atttgtttat ctgctgacaa gcagtcgttt ttctcggatt
     1621 ttttgcaaaa tacgaaattg ttgcttgcaa aataggcttt tttcagtata atcctgccgt
     1681 tcagtcacat tcgtttttta atccttgctt ccacaagttg gtgactggct aaagttggag
     1741 gctgctaacc cgtaaggata gtcaatgcgt cactacgaaa tcgtttttat ggttcacccg
     1801 gaccaaagcg aacaagtacc agcaatgatt gagcgctaca cagcgtctgt aaaagaagct
     1861 ggcggtcaag ttcatcgctt agaagattgg ggtcgtcgtc aattagcata cccaatcaac
     1921 aaacttcaca aagcacacta tgtgttaatg aatgtagaag cacctcaaag tgtaatcgac
     1981 gagttagaaa ctaacttccg ttataacgat gcagttcttc gtaacttaat cgttcacact
     2041 aaagcggcag taactgaagc gtcaccaatg gtgaaagcga aagaaagcaa agttgctgaa
     2101 gcggtagctg aagttgaatc agaggaagca ggcgagtaat ttgccgattg ataattgttt
     2161 aatcttaagc ggaacggttt ccagcttggt taaacaaagc ctaaaccccc ttggtgtgcc
     2221 tcattacagt ttttggttag aacatcgttc aatccaaacc gaagtgaatt tagaacgcca
     2281 agcgtggtgc aaaatccaag tggttttggc aggcaatcaa tttagttatt taacccaaca
     2341 aattaagctc ggtgagagcg tgagaattaa aggttttatc catactcaca aagactttaa
     2401 cggtttaaat cagttagtct tacacaccga acaaattgaa tttatagatc aggagaagcc
     2461 aaatggcacg ttatttccgt cgtcgtaagt tctgccgttt cacagcggaa aatgttgttg
     2521 aaatcgatta caaagatatc gctacattaa agaactacat ttctgaaagc ggcaagattg
     2581 ttcctagccg tattactggt actcgtgcga agtatcaacg tcaattagct cgtgcaatta
     2641 aacgcgctcg ctaccttgcg ttacttccat atactgataa ccaccaataa tcaggagaga
     2701 gtacaatgca agttatttta ttagacaaag ttgctcactt aggttctgtg ggcgaccaag
     2761 taaccgttaa atctggtttt gctcgtaact tcttaatccc acaaggtaaa gcggttatgg
     2821 caactgcggc aaacattgct cactttgaag cacgtcgtgc agaattagaa gcaaaagctg
     2881 cagcagcatt agcaactgca caagaacgtg cagcgaaaat tgcagcatta gcagcagtat
     2941 cagtatctgc aactgcaggt gatgatggtc gtttattcgg ttctgtatca gctaaagaca
     3001 tcgctgaagc attaactgca gcaggtgttg gagttgctaa atctgaagtt cgtttaggtg
     3061 aaggtccact tcgtacaacc ggcgaacacc aagttaaaat tcacttacac cctgaagtga
     3121 atgcaactgt agcagtaaac gttgttgctg aataatcagt agaaatagaa aacccagcga
     3181 aagctgggtt ttttgtttgc tttatttatt ggttagaatc agtgagatat ttctgctgtg
     3241 tcggttaatg cttttgtcca taggcaattt cctttggatt ttttctcaat cagtttcaga
     3301 agtgctaaat gcgactcttc ctcttcatcg tttgctttaa gaataattgc ctcatcggca
     3361 tttaacacaa ttgcagaaaa ttcttcttca atatcaataa cttcacttac cacttcagtt
     3421 tcttcttcgc ctaataatga aatctgaccg ccggtcatca ttaggaatac atcagctaag
     3481 atctccgcat ccagtaacgc cccgtgtagt acacgcttgc tattatcaat gccgagcctg
     3541 tcgcaaagtg catctaaatt attccgtttg ccggggtaca ttcttcgtgc catttgcagg
     3601 ctatcggtta cggtacacat ttctgcggtt tttgccggtg gattaggcag ataagaaaat
     3661 tcttgatcca taaacgccac atcgaagggg gcattgtgaa taattaattc cgcacctttg
     3721 atgaaatcca aaaactcctc ggcaatttca gcaaaaacag gtttatcttg caaaaattca
     3781 ttggtgatgc cgtgaactgc aatcgcctct tcatctacct cacgcggtgg cttgatataa
     3841 acgtgataag ttctgccggt taaacggcgg ttaatgactt caaccgcacc gatctcaata
     3901 atattatgcc caatctgtgg cgggccacct gccatattca ttcctgtggt ttcagtatcc
     3961 agcactactt ggcggataat tttttcttct gtcatattca atttaaaatt gtaggggtga
     4021 aatattttta tcccttaatt ctgcatttga ttaggggcga aacatatttc gcccctacat
     4081 tgttacttcg caatccgttt atatttcact cggtgcggtt gggtggcggc ttcaccgaag
     4141 gttttcttct tccactcttc atagtcggag aagttaccct cgtagaacgt cactttgcct
     4201 tcatcgccgt agtctaaaat atgggtggcg atacggtcta agaaccagcg gtcgtgcgaa
     4261 attaccattg cacagcccgg gaactctaag atcgcatttt ccaacgcacg cagggtttct
     4321 acgtctaagt cgttggtcgg ctcgtccagt aacaacacgt taccgccacg ttgtagtagc
     4381 ttcgccaagt gtaaacgacc acgttcaccg cctgaaagct cgcccacacg tttttgctga
     4441 tctacgcctt tgaagttgaa acgccccaca taagcacggc ttgggatttc aaagttacca
     4501 atcgtgagaa tatcctgccc gttcgacacc tcttcccaca cggttttctt atcgtccatc
     4561 gcatcacgga actgatcgac cgatgccatt accaccgttt caccaagcac gattgaacca
     4621 gaatccggct gttctttacc agaaagcata cggaacaagg ttgatttacc cgcaccattt
     4681 gccccgataa tcccgacaat cgcccccttc ggaatagcga acgacaagtc gtcaatcaaa
     4741 gtgcgatcgc cgtaagattt agtcaggttt tgtacctcga tcactttatc acctaagcgt
     4801 ggacccggtg gaataaagag ttcgttagtc tcgttacgtt tttgatattc gcctgagtta
     4861 agctcttcaa agcgtgccat acgagcctta cttttcgctt ggcgaccttt tggattttgg
     4921 cgtacccatt ccaactcttt ctcaatcgat ttttgacgag ccgattccgc cgcttgctct
     4981 tgtgccaagc gtttttcttt ctgctccaac caagaagagt agttaccttc ccaaggaatg
     5041 ccttcaccac ggtcaagctc taagatccag cccgcaacgt tgtctaagaa gtaacggtcg
     5101 tgggtaattg ccactacggt gccttcgtag tcgtggagga aacgctccaa ccacgccacc
     5161 gattccgcat ctaagtggtt ggtcggctca tctaacaata acatatccgg tttttccagc
     5221 aacagacggc aaagtgccac acggcggcgt tcaccccctg ataaatgctc gatttttgcg
     5281 tcccaatccg gcagacgtaa cgcatcggca gcacgctcta actggttttg aaggttatgg
     5341 ccatcgtggg cttgaataat cgcctctaat tccgcctgtt ttgccgctaa tttgtcgaaa
     5401 tcggcattct catcagcata catcgcatag acttcatcaa gctgggttaa ggcgtttttc
     5461 acttccgcaa ccgcttcttc caccgcttca cgcacggttt gctgtggggc aagtttcggt
     5521 tcttgtggaa gatagccgat tttaattccc ggttgtggac gagcctcgcc ctcgaactct
     5581 ttatctacgc ccgccataat gcgtaaaagc gttgatttac ccgccccgtt taagccgagc
     5641 accccgattt tcgcccccgg gaaaaagctc agggaaatat ctttaagaat gtgacgcttc
     5701 ggcggcacga ctttgccgac acggtgcatc gtatatacaa attgagttga cattggttgt
     5761 attcctcaca aaaaaattcg ccttatttta cttgaattta cagccaaaat atagggtggt
     5821 tttggctttt ggtaaagtaa ccttttccaa tagagatgtt atttgaggct gcatagtact
     5881 atgcatttct cttaccttga caggtaagag aaacaaatcc tgaaggaaaa aaattttaag
     5941 gaagttattc tatgcaatat gtatgtccaa aaacactcaa attattcaac agcattgttg
     6001 gttgttataa gggagaagat taattctcta cctcatcaag gcttgatttt agatttaata
     6061 tccatttcta aagatgattt agatgatatt ttggagggta atactga
//
LOCUS       DANLUQ010000142         5866 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.143, whole genome shotgun sequence.
ACCESSION   DANLUQ010000142 DANLUQ010000000
VERSION     DANLUQ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5866
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.143"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..211)
                     /locus_tag="PXB27_002107"
     CDS             complement(<1..211)
                     /locus_tag="PXB27_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346856.1"
                     /translation="MIQNDPRDAIILSLLANAVYGGHNNSQISYQETEEVNLYARLRQ
                     VEGDVREIKSNYLTKEAFYKAGGSTL"
     gene            complement(216..596)
                     /locus_tag="PXB27_002108"
     CDS             complement(216..596)
                     /locus_tag="PXB27_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346857.1"
                     /translation="MNNKRIKPEQFADFITQRTGRNLTCPICNSQEHYLHDGYDTFTE
                     LCGEKIIGVPTIPYRIEPSPDEVMKFAAPEFHSLHFNEREGTKYWLDQKARTAVIVTC
                     VCCNNMLFFDRERILEWIKGSQNE"
     gene            complement(647..1306)
                     /locus_tag="PXB27_002109"
     CDS             complement(647..1306)
                     /locus_tag="PXB27_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HDL5346858.1"
                     /translation="MTTLGSRIKAYREQLGISQYEVAERCTSIDNRDKNNKWGQSRIA
                     NYERNNRTPDLDDIEILSKVLGVSPETLAFDTNVQLARPTKSNSYPLISNIQAGLWTE
                     AFDFKDSEGYDYIDTEIDAGPDAFFLRISGMSMEPKFSEGDLVLIDIRKRPHPGDYVA
                     AVNGTGEATLKRYRELGEWSESGNPHFELIPLNPDFPTLSSMKQDIRIIGVAVEHRSY
                     L"
     gene            1436..1642
                     /locus_tag="PXB27_002110"
     CDS             1436..1642
                     /locus_tag="PXB27_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005656653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL5346859.1"
                     /translation="MNKIAEIRNQIGISQAQLAVQIGWGQPRIANYETGNRTPSIYVA
                     QKIVEGLNELGANVRIDDVFPVGN"
     gene            1663..1923
                     /locus_tag="PXB27_002111"
     CDS             1663..1923
                     /locus_tag="PXB27_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346860.1"
                     /translation="MPRNELTKNARAIADLIHRKSATVTHKKLARAIGLSESQFSRTF
                     ADNVEMVAVIIDYLSIELADKDELLELKEKRYKRINLEEATT"
     gene            2087..2257
                     /locus_tag="PXB27_002112"
     CDS             2087..2257
                     /locus_tag="PXB27_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346861.1"
                     /translation="MKNQRNDTQKSSLWQEFEYRKRLKNLIESGLSVAEIETRSQQIY
                     EELKSECTTEAN"
     gene            2232..3101
                     /locus_tag="PXB27_002113"
     CDS             2232..3101
                     /locus_tag="PXB27_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDL5346862.1"
                     /translation="MNAQPKQINPILKLHPKQSEAKKVSVDDGYTQIPNELLKAILRS
                     GVLGWKGSYLLATVLKTLSWHKESDWFTHSQVCEMMNIEPTKYHINQLSAARKELIRE
                     NILFEDGKETGVNLSVFEWKMVYPEKVGSSRNNRELIPEKVGNGYPEKVGNTKETITK
                     EKINNEFTNVNSVQQPETSLPKKTKSEPVDYQGVMEEFNRAVEDTPIPQIRAMSDERK
                     RSVHALAKILRKEFGGYTRQHFADYFNDFVRQASSRKDRFYFGGLDGSAWVANFGYII
                     RSKTFYKTWEDSL"
     gene            3098..4459
                     /locus_tag="PXB27_002114"
     CDS             3098..4459
                     /locus_tag="PXB27_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
                     /protein_id="HDL5346863.1"
                     /translation="MSQNLKNITYEVEYLLVGALLKSGLNSKARDVLSWLEPEMFATF
                     QLGVIYEAIRKQALKDNVIDMLLLNTDYGQDFATLAEIMKNTISGANLDGYAEKVRQY
                     HQRREAQRVFLDVAGELQNARDEQLDAITSTGLAHLSKLLQRGGKVKPIDMNDLLEGY
                     LELFQERAKPSFKERLLFTGIEALDGKLGGINDTDICIVAGRAGNGKTETAITFTKNI
                     IENKGSVLFFSLEMSKEQIMDRLIASASGVNSVKLRNPEMMNDEDFARMGTAIQPLQN
                     QQLYIVDKSGLTAEEIVAIAESHIQEYGKVSAVVIDYIGLVRHGKLDGKINRTYQIGE
                     SMERFKTFCKNNHTPMILLAQLNRNADGSRPTNADLRDSGSLEQDASQIIMVHNQRNK
                     DGEPAPYTEWIVTKNRFGANGTVYMQFQQGRFVECDQAEAREYFQPKETKTSSNKRYG
                     AMQ"
     gene            4456..4992
                     /locus_tag="PXB27_002115"
     CDS             4456..4992
                     /locus_tag="PXB27_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HDL5346864.1"
                     /translation="MTDFDKNIKYKIIYADPPWKYKDSGCNGSAENHYTTMNIKEICD
                     LPVQDISDKDSILFLWVTYPMLPEGLKLIEAWGFNYKTIGFQWVKTNKKNKNSFFFGL
                     GRWTRGNTECCLIATKGKPSRVSNKVSQLIIEPIQHHSKKPDIVREKIVELMGDLPRI
                     ELFARNKTQGWDVWGDEV"
     gene            5083..5298
                     /locus_tag="PXB27_002116"
     CDS             5083..5298
                     /locus_tag="PXB27_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346865.1"
                     /translation="MITYKCPKCNGELEDLSINDEWGWFLDEPYRCNGHYTGRFPNIS
                     RDSTLNRTKSCGYFSKEEVKKVNGTQR"
     gene            5282..5788
                     /locus_tag="PXB27_002117"
     CDS             5282..5788
                     /locus_tag="PXB27_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HDL5346866.1"
                     /translation="MAHSVNDIVKAHGRRYKARLKIQMIKMQGGVLVPLNEREAEALV
                     SLKNGEQYEIEVIRTRNPAFHRKVFAFLNFCFEYWSADKTEWEFFDERKQFDTFRKNL
                     TVLAGFKEITYTLDGRMRVEAQSLSYGNMEQDEFEQVYKALINAAIKHVFNNTKDENR
                     INQLYSFF"
BASE COUNT         1920 a         1041 c         1279 g         1626 t
ORIGIN      
        1 tgagtgtaga gccaccagct ttataaaaag cctctttcgt caaataattt gacttgattt
       61 ctcttacatc gccttcaact tgtcttaatc gagcatataa attcacttct tccgtctcct
      121 gataactaat ttgtgaatta ttatgcccgc cgtatactgc atttgcaagt aaggatagta
      181 tgattgcatc tctaggatca ttttgaatca ttggtctatt cattttgtga gcccttaatc
      241 cattctaaaa ttctctctct gtcgaaaaaa agcatattat tacagcaaac acaagtgaca
      301 ataacggctg ttctcgcttt ttggtctaac caatattttg ttccttcccg ttcattgaaa
      361 tgcaaagaat gaaattcagg agctgcaaat ttcataactt catcaggaga tggctctatt
      421 cggtatggaa tggttggaac accgataatt ttctctccgc ataattccgt aaaagtgtca
      481 tagccatcgt gtaaataatg ttcttggcta ttacaaatag ggcaagttaa attccgccca
      541 gtacgttgcg tgataaaatc cgcaaactgt tcaggtttaa tgcgtttatt attcatttta
      601 acttcctttc tacccaataa aattcaactg ttagctttgg tggactttac aaatagcttc
      661 tatgctcaac agcgacacca ataatacgaa tatcttgttt cattgagctg agtgttggaa
      721 agtcggggtt aagcggtatt agctcaaaat gaggattgcc ggattcagac cattcgccta
      781 actcccgata acgttttaaa gtagcctcac ctgtgccatt aaccgctgct acataatcgc
      841 ccgggtgagg acgtttgcga atatcgatta acaccagatc gccttcacta aatttaggct
      901 ccatcgacat tccggaaatt cttaaaaaga aagcgtccgg tccggcatca atttccgtat
      961 cgatatagtc gtagccctcc gaatctttga aatcaaaggc ttctgtccat aaacccgctt
     1021 gtatattgct gatcaacgga tatgagttcg attttgtcgg tctggcaagc tgaacgtttg
     1081 tatcaaaagc aagtgtttct ggagaaacac caagcacttt gctgagtatt tcaatatcat
     1141 caagatcggg agtacgattg tttctctcat aattagcaat gcgagattgc ccccatttgt
     1201 tatttttatc tcgattatct attgatgtac atctttcagc aacttcatat tggctgattc
     1261 ctagctgttc tctgtaagcc ttaattctac tacctaaggt tgtcatagca ctctcctatt
     1321 agattttcct cattttaaca cgctccgtta tatccttgtg aatcacaaaa tgaaatttac
     1381 aattagttca ttttgtgatt taattaaaag atataaatca caaagagaaa tttgtatgaa
     1441 taagatagct gaaatacgaa accaaattgg tataagtcaa gctcagttag ctgttcagat
     1501 tggttggggg caaccaagaa tagctaacta tgaaactgga aatcgtaccc catctattta
     1561 cgttgctcaa aaaattgttg agggattgaa cgaattaggt gcaaacgtac gaattgatga
     1621 cgttttcccc gttggcaact aatttacccc aagaggcttg caatgccacg caatgaatta
     1681 acaaaaaatg caagagcgat tgcggatttg attcatcgaa aatccgcaac ggtgacgcat
     1741 aagaaactag caagagcaat cggactgagt gaatcacaat ttagccgtac atttgcagat
     1801 aatgtggaaa tggtggcggt gattattgat tacttaagta ttgagcttgc ggataaagac
     1861 gagttactag agctgaaaga gaaacgatat aagcgaataa atctcgaaga agcgacaaca
     1921 tagaagttac caacaatgaa aaatttttta gaaaaattca aatcattcta ccacagtaaa
     1981 aattcaattt ttattagagt ggcaattttt atgctgcttg cgtggtgggt ttttaacaat
     2041 taaaaaacca ccgctggaac ggtggtaatt tacaaggagt taccttttga aaaatcaacg
     2101 caatgataca caaaaaagct ccttatggca agagtttgaa tatcgaaaac ggttaaaaaa
     2161 tctcattgaa agcggtttat ccgtagccga aattgaaacc cgttcacaac aaatttatga
     2221 ggagctgaaa agtgaatgca caaccgaagc aaattaaccc aattttaaaa ttgcacccta
     2281 aacaatcaga ggctaagaaa gtgagtgttg atgatggtta tacacaaatt ccgaatgagt
     2341 tattaaaagc aattttgcga tcgggtgttt taggttggaa aggctcttac ttactggcta
     2401 ccgtcttaaa aacattgtct tggcataaag aaagtgattg gttcacgcat tcccaagtgt
     2461 gtgaaatgat gaatattgag ccgacaaaat accatattaa tcaactttcc gctgctcgaa
     2521 aagaacttat ccgtgaaaat atcttgtttg aagatggtaa ggaaacaggg gtaaatctca
     2581 gtgtttttga atggaaaatg gtatatcccg aaaaagtagg gagttcccga aataataggg
     2641 aattaattcc cgaaaaagta gggaatgggt atcccgaaaa agtagggaac acaaaagaaa
     2701 ctattacaaa agaaaaaata aataatgaat ttactaacgt aaattctgtg caacagcccg
     2761 agacctcttt gccgaaaaaa acaaaatctg agccggtcga ttatcaagga gtgatggaag
     2821 aatttaatcg tgctgtggaa gacacaccga ttccgcaaat tcgggcgatg agtgatgagc
     2881 gaaagcgatc ggttcacgca ttagcaaaaa tcttgcgaaa agaatttggt ggctacacca
     2941 gacaacactt tgccgattac ttcaatgatt ttgtccgaca ggcaagtagc cggaaagaca
     3001 gattttattt tggcgggctt gatggctcgg cttgggtagc aaatttcggc tacatcattc
     3061 gttcgaaaac gttttataag acttgggaag attcgctatg agccaaaatt tgaaaaatat
     3121 cacttacgag gttgaatatt tgctggttgg agcattgctg aaatccggtc ttaactcgaa
     3181 agctagggac gtattgagct ggttagagcc tgaaatgttc gctacctttc aacttggggt
     3241 gatttatgag gctatccgca agcaggcgtt gaaagataac gtgatcgata tgctgttact
     3301 caataccgac tacggacaag attttgccac gttggcagag ataatgaaaa acacgatcag
     3361 cggtgcaaac ttagacggct atgcagaaaa agtccggcaa tatcatcaac gccgagaagc
     3421 ccagcgagtg tttttagacg ttgcaggtga gttgcaaaat gcccgagacg agcagttaga
     3481 tgctatcact tcaaccgggt tagctcacct aagcaaatta ttacaacgtg gcggaaaagt
     3541 gaaaccgatt gatatgaatg atttgctcga aggttatttg gaattgttcc aagaacgtgc
     3601 aaaaccaagt tttaaagagc gtttgttgtt taccggtata gaagcattag atgggaaact
     3661 aggcggtatt aacgataccg atatttgcat tgtagcagga cgtgccggta acggtaaaac
     3721 cgaaacagcc atcacgttta ctaaaaacat tatcgagaat aaaggttcgg tgctgttctt
     3781 ctcgctggag atgagcaaag agcagattat ggataggctg attgccagtg caagcggtgt
     3841 aaattcagta aaacttcgca atccggaaat gatgaatgat gaagattttg cccgaatggg
     3901 aacggcgatt cagcctttac aaaatcagca gttgtacatt gttgataaaa gcggattaac
     3961 cgcagaagag attgtggcga ttgcagagag ccatattcaa gagtacggca aagtgagtgc
     4021 ggtagtgatt gattatatcg gtttggttcg acacggcaaa ctagacggca aaattaaccg
     4081 aacctatcaa atcggtgaaa gtatggagcg gtttaaaact ttctgcaaaa acaatcacac
     4141 accgatgatt ttattggcac agcttaaccg caatgcagat ggcagtcgcc caaccaacgc
     4201 agatttgcga gacagtggca gtcttgaaca agatgcaagc caaatcatta tggtgcataa
     4261 ccaacgcaac aaagacggcg agccagcccc ctatacagaa tggattgtta ctaaaaatcg
     4321 ctttggagca aatggcacgg tttatatgca attccagcag ggtaggttcg tagaatgcga
     4381 ccaggcagaa gcgcgggagt atttccagcc gaaagaaact aaaacatcat caaacaaacg
     4441 ctatggagca atgcaatgac agattttgat aaaaatataa aatacaaaat tatttacgca
     4501 gatccgcctt ggaagtataa ggatagtggc tgtaatggga gtgcagaaaa ccattacaca
     4561 acaatgaata tcaaagaaat atgtgattta cccgtccaag atatttcaga caaagattct
     4621 attctgtttt tgtgggttac ttaccctatg ttacctgagg gcttaaagtt gattgaggcg
     4681 tgggggttca actataaaac aatagggttt caatgggtga agacaaataa gaagaataag
     4741 aattcttttt tctttggctt gggacgatgg actagaggta ataccgaatg ctgtttaatt
     4801 gcaacaaaag gaaagccatc gagggtaagc aataaagtga gccaattaat tattgagcca
     4861 atacaacatc atagtaaaaa gcctgacatt gtcagagaaa agatagttga gttgatgggt
     4921 gacttgccaa gaatagagtt gttcgccaga aataaaaccc aaggttggga tgtttggggg
     4981 gatgaggtgt gattgtttca agctattgtt acccaaagtg cggtagggtt ttattaaaaa
     5041 ggttggcggt tgtgaaggat aacgaaggtt gggacggttt agatgattac ttataaatgc
     5101 cccaaatgca acggagagct tgaagattta agtattaatg atgaatgggg ttggttttta
     5161 gatgagccgt atcgttgcaa cggacactat acagggcggt ttccaaacat tagccgagat
     5221 agcacgttaa atcgcacaaa atcctgtggg tactttagca aggaagaggt taagaaagta
     5281 aatggcacac agcgttaatg acatcgtaaa agcccacggc aggcgttata aagcaagatt
     5341 gaaaatacag atgataaaaa tgcaaggagg cgttttagtc ccccttaatg agcgtgaagc
     5401 tgaagcattg gtatcactta aaaatggcga gcaatatgaa attgaggtta ttcgcactcg
     5461 caatccagcc tttcatcgga aggtcttcgc ctttttaaat ttttgctttg aatattggtc
     5521 ggcagacaaa accgaatggg agttttttga tgagaggaaa cagtttgata ctttccgaaa
     5581 aaatctgact gttctggcag gatttaaaga aatcacatac accctagatg ggcgtatgcg
     5641 tgtagaagct caaagtctta gttacggaaa tatggaacaa gacgagttcg agcaggttta
     5701 caaagcattg attaatgcag ctatcaagca cgttttcaac aataccaaag atgagaatag
     5761 gataaatcag ttgtattcgt ttttttaatt aaggcgaaat cgccatattt aattaggttt
     5821 aattgcagga gattaaaatg aaaaagaatg ttattagata ttggct
//
LOCUS       DANLUQ010000143         5782 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.144, whole genome shotgun sequence.
ACCESSION   DANLUQ010000143 DANLUQ010000000
VERSION     DANLUQ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5782
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.144"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..706)
                     /locus_tag="PXB27_002118"
     CDS             complement(<1..706)
                     /locus_tag="PXB27_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005712160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL5346867.1"
                     /translation="MSIYRRKANGPWWVDITTPNGKRIKRSTRTLVKREAQQYHDKLK
                     QEMWAEDKLEEKRKYIFEDALLHYVRAAEELKDKATKKRHAEYWLSKFAGRELSSLTA
                     QEIILNIPKKNANTKKPLSHSTQNKYVKSLSRVLNIAHKLGMLDAVPHIPKKKEPPIR
                     VRWITKEQAKQLIDKLSSDWMKSICKFALMTGARRTEILTMTWDKIDLVRKVAIVTND
                     VAKSGKARSLLLNQEAI"
     gene            complement(610..933)
                     /locus_tag="PXB27_002119"
     CDS             complement(610..933)
                     /locus_tag="PXB27_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5346868.1"
                     /translation="MEHLTINQVAKELNCHPNTVRQHLADWGFFKMVGSEVWRINRED
                     LERAKKRAGIDPIRKRSDNDRGLSLSVEENEKCRSTDEKQTVRGGLISQPQTARELRE
                     ALARL"
     gene            complement(1014..1181)
                     /locus_tag="PXB27_002120"
     CDS             complement(1014..1181)
                     /locus_tag="PXB27_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDL5346869.1"
                     /translation="MQFTYGSICSGIEATSIAWHGLGKPLWFSEIEPFPCAVLAHRFP
                     NVPNGNGMAVP"
     gene            complement(1189..1563)
                     /locus_tag="PXB27_002121"
     CDS             complement(1189..1563)
                     /locus_tag="PXB27_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346870.1"
                     /translation="MHIEDKIAWWLANGETGVSSKTMAFYLGYGIRPKIEGYPHDVSD
                     FRRCFLLLETVPFLRNRIEKMAELGKVWAALAKEWHTLEALYNEEECQIRCPKTYAKL
                     REILEANEENVVRIGNLSISIG"
     gene            1713..1940
                     /locus_tag="PXB27_002122"
                     /pseudo
     CDS             1713..1940
                     /locus_tag="PXB27_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
     gene            1965..2360
                     /locus_tag="PXB27_002123"
     CDS             1965..2360
                     /locus_tag="PXB27_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5346871.1"
                     /translation="MKIHTQIRKEILSLLEAKLTDIEHFYNGQPNFIDIDEQQLAISV
                     YLDEINRQELTLCDEQWTAQLNITIYLKSVDEAEDELDEWAEKIREVVETYSAFEHLE
                     GISLSQYQYEQDQNQRTWHSATLIFDVEY"
     gene            2392..3033
                     /locus_tag="PXB27_002124"
     CDS             2392..3033
                     /locus_tag="PXB27_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346872.1"
                     /translation="MAKTTKVQGTKFRIGIGREAQKAITAITLATATLTIATSGYKKG
                     DAIEITGCGQLDGIYPVLSVTGDQVKLCEEVNWTGKDLPANYTKAKAALVQYSDQFCA
                     VKNIEKSDDTLSTEDVTTVCSEGTETEPGEIEFGSIKLSFFHKPSTEMQTRLRKLFYD
                     KSTFAYKLELPDNHGTTYGEGFIEAGNGFSGEVKGKYEGSVSIKPSKRDYLLV"
     gene            3116..3517
                     /locus_tag="PXB27_002125"
     CDS             3116..3517
                     /locus_tag="PXB27_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346873.1"
                     /translation="MTLREKLLANKPKLQSIEINGETYYLREATVGDMNKQIFETRSW
                     LIQQAEQENVELPAEDDETFDEALNRFGEKYRLAQSVAYRLCDENGALLFNPLNIDDL
                     NAIAELDSKVIIDFNQAVSAPKDSASEESSK"
     gene            3562..3792
                     /locus_tag="PXB27_002126"
     CDS             3562..3792
                     /locus_tag="PXB27_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HDL5346874.1"
                     /translation="MPERHFAEYQLFYQEQPFGLWREDYRAAQLAHLTAMINRDPKGK
                     APELSEFMPFFNRADEVEANEDDGVADYLAKR"
     gene            3860..4126
                     /locus_tag="PXB27_002127"
     CDS             3860..4126
                     /locus_tag="PXB27_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDL5346875.1"
                     /translation="MATINDAFSFRTNTEIKNTAFDVIKNYGMTPSQVFNMFLTEIAK
                     TKTIPLSLNYQPNLETKLAMQEAKSGKNEVYASLEAFHKAMLAE"
     gene            4130..4399
                     /locus_tag="PXB27_002128"
     CDS             4130..4399
                     /locus_tag="PXB27_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HDL5346876.1"
                     /translation="MLQISPTNAYKRDFKKIAAELVGSSEYVEVMYCLINQLPLAEKY
                     RDHPLQGEWQGFRDCHIKPDLVLIYAVEDNLLRLVRLGSHAELFG"
     gene            4474..4737
                     /locus_tag="PXB27_002129"
     CDS             4474..4737
                     /locus_tag="PXB27_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346877.1"
                     /translation="MKNVLLFIVHFFLIAMLSIIPILIILGAIERDPYYTAWIFLSVF
                     LGIGALLYFATIVSKLINKGKTQTLEEKRYAEYWNNVKVNIPK"
     gene            4797..5507
                     /locus_tag="PXB27_002130"
     CDS             4797..5507
                     /locus_tag="PXB27_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HDL5346878.1"
                     /translation="MSGLGKLTVTLELENAKFQSAMTKSDYEAQKFAKNFIQNMDRAR
                     NHAKEFADRSTQYLKNIEQAAKNLNKNSEFSFLSTIGGHYQSMSSGILSAAKAYTDIQ
                     NKMKLVSGSSAEAAKRLTDVFDIATKTSQSTEAVSGVYQTFAQNAQSLGLAQKDVAEL
                     TKTVSQAVAASGASSSAASNALTQFGQSLLMGKMKAQEFNSLITQTPTIIQAMAKGLG
                     MTMAEFKAAVYRAKSLLG"
     gene            5485..5775
                     /locus_tag="PXB27_002131"
                     /pseudo
     CDS             5485..5775
                     /locus_tag="PXB27_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484718.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
BASE COUNT         1808 a         1181 c         1120 g         1673 t
ORIGIN      
        1 taattgcctc ttgattcagt aatagcgatc gtgccttacc tgatttagcg acatcattag
       61 tcacgattgc tacctttctt acaagatcta tcttatccca agtcattgtc aaaatttctg
      121 tccgtctggc tccagtcatc aaagcaaact tacagataga tttcatccag tctgaactta
      181 atttatctat tagctgtttt gcttgttctt tggtgatcca gcgtacacga attggcggct
      241 ctttcttttt cggtatgtgt gggactgcat ccaacatacc aagtttatga gcgatattta
      301 atactctcga taaagatttc acatacttat tttgcgtact atgagataga ggctttttcg
      361 tatttgcatt ctttttcggg atatttagga taatctcctg agctgttaaa gagctgagtt
      421 cacgaccggc aaatttggat aaccagtatt cagcatgtcg cttttttgtg gctttatctt
      481 ttaattcttc ggccgcacga acgtaatgga gcaaagcatc ttcaaagata tattttctct
      541 tttcctcgag tttatcctct gcccacattt cttgcttgag tttatcgtga tactgctgag
      601 cttcacgttt tacaagcgtg cgagtgcttc tcttaattct cttgccgttt ggggttgtga
      661 tatcaaccca ccacggaccg tttgcttttc gtctgtagat cgacattttt cattctcctc
      721 gaccgacaga gataaccctc ggtcattatc agatcgtttt cttattgggt caataccggc
      781 tctctttttt gctctttcca aatcttctcg gttgatccgc caaacttccg agcctaccat
      841 tttaaaaaat ccccaatccg ccaaatgttg gcggactgta tttggatggc aatttaattc
      901 ttttgccact tgattaatag taagatgttc cattcattac tttctccaaa ataaatccct
      961 ctaaacgagg gattgtacta aataatcgtt aagtctttgc cttatccact gcatcaaggc
     1021 acagccatac cgtttccatt tggcacattg gggaaacggt gagctaatac cgcacagggg
     1081 aatggctcaa tttcagagaa ccacaaaggc ttacctaaac cgtgccacgc tatacttgta
     1141 gcttcgatac cgctacaaat tgagccgtag gtgaattgca ttttttacct atcctatcga
     1201 aatactcaaa ttcccaatac ggacaacatt ttcttcgttg gcttctagga tttctctcaa
     1261 cttagcatag gttttggggc atcttatttg acattcctct tcgttgtaga gagcctctaa
     1321 tgtgtgccat tcttttgcaa gtgcagccca aactttccca agttcagcca ttttttcaat
     1381 cctatttcgc aaaaaaggga ctgtctccaa caacagaaag caacgcctga aatccgatac
     1441 atcgtgtggg tagccttcta tttttggtct tattccgtaa ccaaggtaaa aagccattgt
     1501 tttactgctt acccctgtct ctccattggc aagccaccaa gcgattttgt cttctatgtg
     1561 catttttcct ccaaaaatta accgcttgcg gaacaagttc tacaagcggt tgtcaagtgt
     1621 tcatttgttc aatgaacagt tcattgtgaa taacaaaacc gcttgaatta caagcggtct
     1681 attttgttgc gtaaatttac tgttagcgtt gagccataac cgactacgac cgctggcccg
     1741 aagatattga cggcgagggg gacggcttct cgcttgcctc caaacggacg accactttta
     1801 tgtcagccgg aatgacttta gtggagagtt cgcctggtaa agatattgtc gatctcaaac
     1861 atatcccgaa aagtagccac gaagccccac cgactaccgg tattttaagc ctctacaatc
     1921 gaggcgaccg cagacgcttt agcgaaacag ttgataggta agctatgaaa atccataccc
     1981 aaatccgaaa agaaatcctt tcattattag aggcaaaatt aacggacatc gagcattttt
     2041 ataacgggca accgaatttc attgatattg acgaacagca acttgccatc tctgtttatc
     2101 ttgatgagat taaccgtcag gagcttacct tatgcgatga acagtggaca gctcaactta
     2161 acatcacgat ttatctaaaa tcagttgatg aggcagaaga tgaattagat gagtgggccg
     2221 aaaaaatccg tgaagtggtt gaaacatatt ctgcgtttga acatttagag ggcatcagcc
     2281 tttctcaata tcaatacgaa caagatcaaa atcaaagaac ttggcactca gccacattga
     2341 tttttgatgt tgaatactaa caagcaaata atccttatag gagaatctac aatggcaaaa
     2401 acaacaaaag tccaaggcac aaaatttaga attggcattg gacgtgaagc ccaaaaggca
     2461 attacggcta tcactttagc tacagcaacg cttactattg ccacatcagg ctataaaaaa
     2521 ggcgatgcaa tcgaaattac cggttgtggt cagttagacg gcatttatcc tgtattatct
     2581 gtaacaggcg atcaagtcaa attgtgcgaa gaagtaaact ggacgggtaa agatttaccg
     2641 gctaattaca ccaaagccaa agcagcttta gtacaatact cagaccaatt ctgtgcggta
     2701 aaaaacattg aaaaatctga tgatacgtta agtaccgaag atgttacaac ggtctgctcg
     2761 gaaggtacag aaaccgaacc cggagaaatt gaatttggct caattaaact gagctttttt
     2821 cacaagccaa gtacggaaat gcaaactcgc ttacgcaaat tgttttatga caaatccacc
     2881 tttgcgtaca aattagaatt acctgataac cacggcacaa cctatggcga aggtttcatt
     2941 gaggcgggta acggctttag tggcgaagtt aaaggcaaat atgaaggctc ggtatcaatt
     3001 aaaccaagca aacgtgatta tttgctagtg taattacaaa atattgctcc aaatcgaccg
     3061 cttgtaatat tgcaagcggt cttttttatc ctaaatttta caaaaggaat cgacaatgac
     3121 actccgtgaa aaactcctcg ccaacaaacc aaaattacaa tctatcgaaa tcaacggtga
     3181 gacctactac ctgcgtgaag ccactgttgg cgatatgaat aagcagattt ttgaaacccg
     3241 aagctggctc attcaacaag ctgaacaaga aaatgttgaa ttaccggcag aagatgatga
     3301 aacctttgac gaagctctca accgttttgg cgaaaaatac cgccttgctc aatcggttgc
     3361 ctaccgttta tgtgacgaaa acggtgcatt actgtttaac ccacttaaca ttgacgatct
     3421 caatgcgatt gccgaattag acagtaaagt gattatcgac tttaaccaag ccgtgtccgc
     3481 cccaaaagac tcagcgagcg aagaaagttc caaataaccc tttcgctcgc actgggtaaa
     3541 acccttgaag aaatcgaaca aatgcctgaa cgccactttg ccgaatatca gcttttttat
     3601 caagagcaac cttttgggct ttggcgtgag gactatcgtg ctgctcaact tgcccactta
     3661 accgcaatga tcaaccgtga cccaaaaggc aaagcccctg aactctcgga atttatgccg
     3721 ttttttaatc gggctgatga agtagaagca aatgaagatg atggcgttgc ggattatttg
     3781 gcgaaaaggt aaatctcttg tatttcattg taattctgtc tataattggt gtaatacata
     3841 gaaatacagg agagataaaa tggcgactat caatgatgct ttcagtttta gaacaaatac
     3901 cgaaataaaa aataccgcat ttgatgtaat taaaaactat ggaatgacac cctctcaggt
     3961 gtttaatatg tttttaaccg agattgcaaa aacgaaaact attccgttaa gtttgaatta
     4021 tcaacccaat cttgaaacaa aattggcaat gcaagaagca aaatcaggta aaaatgaagt
     4081 ttatgcctca cttgaagcat ttcataaagc aatgttagcg gagtaaataa tgctacaaat
     4141 ttcgccgaca aacgcatata aaagagactt taaaaagatt gcagccgaat tagtcggtag
     4201 ttcggaatat gtggaagtaa tgtattgcct aataaaccaa ttaccattgg cggaaaaata
     4261 tagagatcac ccactacaag gtgaatggca aggctttaga gattgccata ttaagcctga
     4321 tttagtgttg atttacgctg ttgaagataa tctgctccgc cttgttcgtt taggatcgca
     4381 cgctgaatta tttggataat ttctgtaaaa actaaccgct tgcgattttg tgctgtgggc
     4441 ggtatagttg gaatattaac aaaggaggga aaaatgaaaa atgtacttct atttatcgta
     4501 cactttttct taatcgcaat gctctcaatt atcccaatat taattattct tggagcaatc
     4561 gaaagagatc cttactatac tgcgtggatt ttccttagcg tatttttagg cattggagca
     4621 ttgttatatt ttgcaaccat tgtttctaag ttaattaaca agggaaaaac acaaacatta
     4681 gaagaaaaac gatatgcaga atattggaat aatgtaaaag tcaatattcc caaatagtct
     4741 aactgataaa tgaccaaaag ctcgctttat gcgggctttt ttattggaga aaaacaatga
     4801 gtggcttagg aaaattaacc gttaccttag agcttgaaaa tgcaaaattt cagtccgcaa
     4861 tgacgaaatc tgattatgag gcacaaaaat ttgccaagaa ctttatccaa aatatggata
     4921 gagctagaaa tcacgccaaa gaatttgccg atcgctcaac ccaatattta aaaaacattg
     4981 agcaagcggc aaagaacctc aataaaaatt ctgaattttc atttttatcg actattggtg
     5041 gacattatca atcaatgtct agtggcatat tgagtgcggc aaaagcctat actgatattc
     5101 aaaataaaat gaaattagta agcggtagtt cagcagaggc agctaaacga ttaactgatg
     5161 tgtttgatat tgcgactaaa acaagccaaa gtacagaggc tgtttcaggt gtctatcaaa
     5221 catttgctca aaatgcccaa tctctcggac tggctcaaaa agatgtagca gaattaacta
     5281 aaacggtctc tcaagccgtt gctgcaagcg gcgcaagcag ttctgcagca agtaatgccc
     5341 taacccaatt tggtcaatca cttttaatgg gcaaaatgaa agcccaagaa tttaactcgc
     5401 tcattactca aaccccaacc attattcaag caatggcaaa aggtttggga atgacaatgg
     5461 cagaatttaa agcagcggta taccgggcaa aatctttact tggataacat tcaagggcaa
     5521 ggttttgaga gagtggatac accccaaatc ggcgatgtga ttttaataca ggtcggttct
     5581 gatgtgccga atcacgcagc gatttatatt ggtgaacaaa tggtaatcca ccatagcccg
     5641 aaccgattat ctaagcgtga tttatatgac ggttattggt tacgccatac ccacagtatt
     5701 tggcggcata aactggcgga tagattggat tttgacggca tatttaatga tataggagaa
     5761 caatatgaaa tttagtaaat at
//
LOCUS       DANLUQ010000144         5728 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.145, whole genome shotgun sequence.
ACCESSION   DANLUQ010000144 DANLUQ010000000
VERSION     DANLUQ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5728
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.145"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..518)
                     /locus_tag="PXB27_002132"
     CDS             complement(<1..518)
                     /locus_tag="PXB27_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL5346879.1"
                     /translation="MLDSLKEVNQKLDKKADKVGGGTKEDALVTLDTLIASGTVKAET
                     AMSLLPTLQKGAVATGASSEDMAKIAISSMQQFGIKEEDIGRALDMAVAAGQAGSFEL
                     AYMASWLPQQMAAAKQAGLSSIEGFERLLIANQQARVTAGTSDEAGNNLVNLLGKITA
                     KETNERFKNIEY"
     gene            617..868
                     /locus_tag="PXB27_002133"
     CDS             617..868
                     /locus_tag="PXB27_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL5346880.1"
                     /translation="MAPTISDGDQMLVDFSKKEARGDDKIYLVQNGESVWVKRVRKVW
                     DYVELISDNESYRPIRITADEAQNLQIIGQVVHIGHSLV"
     gene            complement(1064..1264)
                     /locus_tag="PXB27_002134"
     CDS             complement(1064..1264)
                     /locus_tag="PXB27_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HDL5346881.1"
                     /translation="MQYVLDLTGYACPLPLLMAKKAMNDLKKGDSLEILLNQQSSLTD
                     FELLAKEQGWLCELQAVENVGY"
     gene            complement(1323..1835)
                     /locus_tag="PXB27_002135"
     CDS             complement(1323..1835)
                     /locus_tag="PXB27_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017357669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HDL5346882.1"
                     /translation="MQQLEPQTFASWAEPIDMLYACHSKVKSFCKQLQILPDYLEKNG
                     VNQAVKNDVQQILNYFNISAPLHHEDEEDDFFPELIKVQPQAQAEVDELERQHVHLHK
                     NWDYLSAQLEDLLAGKRENVDRDLIERFVAGYDVHIAIEEPLFELGREHLAQDKLSAM
                     GKVMADRRKL"
     gene            complement(1848..3002)
                     /gene="tgt"
                     /locus_tag="PXB27_002136"
     CDS             complement(1848..3002)
                     /gene="tgt"
                     /locus_tag="PXB27_002136"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDL5346883.1"
                     /translation="MKYELKTTSGNARRGRLTFNRPKGEFHVETPAFMPVGTYGTVKG
                     MTPEEVKATGAQILLGNTFHLWLRPGQEVMKMHGDLHDFMQWHGPILTDSGGFQVFSL
                     GKLRKIKEEGVTFQNPISGEKIFLSPEKSMEIQYDLGSDIVMIFDECTPYPATFDYAK
                     KSMEMSLRWAKRSRDRFDELENPNALFGIIQGGIYEELRKVSVEKLVEIGFDGYAVGG
                     LAVGEPKEDMHRILEYVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNA
                     RNGHLFVTNGIVKIRNAKYRADTTPLDPECDCYTCKNYTKAYLYHLDKCGEILGARLN
                     TIHNLRYYQRLMAEIRQAIEEDRFDDFVVEFYARIGKEVPPLQSEVKEQK"
     gene            complement(3061..3201)
                     /locus_tag="PXB27_002137"
     CDS             complement(3061..3201)
                     /locus_tag="PXB27_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346884.1"
                     /translation="MKKLLLCVGILVLAACSSTGVGGSIGAGGGSNGVGIGLGVGTDF
                     RF"
     gene            3295..3867
                     /locus_tag="PXB27_002138"
     CDS             3295..3867
                     /locus_tag="PXB27_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1439 domain-containing protein"
                     /protein_id="HDL5346885.1"
                     /translation="MKILRKFAAVCVVSMAMLSANANLLTISEQEINDYLATKLAEKI
                     PLKDKVGIPKLFELDYRLHSLTSQIGRTEEKKVAISGVVDGLLNAKGKKYAASIHLNM
                     DTTPYYDPQKGALFLKDVRLLSWSATPAKYQAELQMFLPVLADGLSSFLNSTPVYTLD
                     ENKTKEAMVKKFGKAIVVENGNIKLETTIF"
     gene            3969..4187
                     /gene="cspD"
                     /locus_tag="PXB27_002139"
     CDS             3969..4187
                     /gene="cspD"
                     /locus_tag="PXB27_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein CspD"
                     /protein_id="HDL5346886.1"
                     /translation="MDIGIVKWFNNVKGFGFITSDVCEGDIFAHFSEIQAEGYRSLKV
                     GQKVQFELVQGERGASASNITPISEKTL"
     gene            4277..5191
                     /locus_tag="PXB27_002140"
     CDS             4277..5191
                     /locus_tag="PXB27_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL5346887.1"
                     /translation="MPSSYQLVLINLLYIFGIGLNIVLLRFLSLHFDPLNNNGIRFLV
                     GGFTLLFFILWRFRTSLSQLIKTPKLIAISLFVGLMMSANMYFWLKGVKITNAVTVSI
                     FGVLAMPFGVIIAAIFFQDERERIKSKAFWFGSILTIIGSLGFIWHGKTLSIGEGFIL
                     GSVFLFLSIIIRNIQNLVVKFARELNVLTLSCVTSLTTSLSSLLLSQQAGKFNELFTA
                     PNWLLFSLMVAGIYAIMIGMVLTFHIIQTQGLITYQVLELLMPVSTAIIAYILLDERI
                     SIVQMGFACIVILGSSIALKLVKYPKHY"
     gene            5310..>5728
                     /locus_tag="PXB27_002141"
     CDS             5310..>5728
                     /locus_tag="PXB27_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HDL5346888.1"
                     /translation="MYKNLSLLSPTYPFDLEFIAGLTQCEKGNYLDSIVDRQQGMHGY
                     MLQLTTFGRGSVFDGKHVFTAHRGQLLLFTPNAVQHYHRHPESQYWHYKWVYFQPNPK
                     WNKWLNWSDMEEKIGRITISDDRYFQEISQLFSKIEL"
BASE COUNT         1647 a         1199 c         1056 g         1826 t
ORIGIN      
        1 ttatattcaa tatttttaaa acgctcattt gtttctttag ctgtaatctt accaagcaga
       61 tttaccagat tattacctgc ctcatcgctt gtaccggcag tgacacgagc ctgttggttt
      121 gcaattaaaa gtcgctcaaa gccctcaata ctactcaaac cggcttgttt tgcagctgcc
      181 atttgttgag gcaaccaact tgccatatat gcaagctcaa aactacctgc ttgcccggct
      241 gcaacagcca tatccaatgc ccgcccaata tcctcttctt tgatgccaaa ttgttgcata
      301 gaggagatcg caatttttgc catatcctcc gagcttgccc ctgttgccac cgcccctttc
      361 tgcaaagtag gtaatagact cattgcggtt tcggctttga ctgtaccgga ggcaatgagc
      421 gtatcaagcg taactaacgc atcctctttt gtgccacccc caaccttatc cgccttttta
      481 tcaagttttt ggtttacttc tttgagcgag tctaaaatat cgtccagctt ttccttaaga
      541 gccttattca gacagcctat taagcctagg cgtaaaagcc cataattgtg gcgtattttg
      601 ggctaatggc acatcaatgg ccccgactat cagcgatggt gatcaaatgt tagtggattt
      661 cagcaaaaaa gaagcccgtg gagacgataa gatttatctt gtgcaaaatg gtgaaagcgt
      721 ttgggtaaaa cgtgtaagaa aagtgtggga ctacgttgaa ttgattagcg ataatgagtc
      781 ctaccgccca atccgaatta cagcagacga agctcaaaac ttacaaatca tcgggcaggt
      841 agtccacatc ggacactctt tagtttaaaa cagatttaaa atagttttaa aaccgatcaa
      901 acctatgcaa aaaatatcgc aaaaactgcc catttttttc taatcctgct cattttgttt
      961 tttgcatagc cccaaccaaa caataacggc gaacccttgt attcacgggg ctcgccgtta
     1021 tttttccgcc caaatttttt tctttctctc tatgcaaaaa agatcagtac cccacatttt
     1081 caaccgcttg tagctcacaa agccaacctt gctctttggc aagcagttca aaatcggtaa
     1141 ggctactctg ctgattgagt aaaatttcca agctatcgcc ctttttaaga tcgttcatcg
     1201 ctttttttgc cattaataac ggcaaggggc aggcatagcc ggttaaatct aaaacatatt
     1261 gcatagcgaa atagggaata aagaaaaagg gcgaatattg aaattcgccc aatcacaaaa
     1321 gattagagtt tacggcggtc tgccatcact ttgcccatag ccgataattt gtcttgagct
     1381 aaatgctctc gcccaagctc aaagagtggc tcttcaatcg caatatgcac atcataacct
     1441 gccacaaaac gctcaattaa gtctctatcc acattttcac gtttacctgc gagcagatct
     1501 tcaagctgtg ccgacaaata gtcccaattt ttatgtaaat gaacgtgctg gcgttccagt
     1561 tcgtctacct ctgcttgagc ttgtggttgc accttaataa gctcagggaa gaaatcatct
     1621 tcttcatctt cgtgatgtaa aggggcagaa atgttgaagt agttcaaaat ctgctgcacg
     1681 tcattcttca ctgcttggtt taccccgttt ttctcaaggt aatccggcaa aatttgcaac
     1741 tgtttacaga aactttttac tttgctgtgg caagcataaa gcatatcaat cggctctgcc
     1801 cagcttgcaa aggtttgtgg ttcaagttgt tgcatataca ctccttgtta tttctgttct
     1861 tttacttcag attgtaaagg tggcacttct ttgccaattc gggcataaaa ttccactaca
     1921 aaatcatcaa aacgatcttc ttcgatggcc tggcgaattt ctgccattag gcgttgatag
     1981 tagcgtaaat tatgaatggt gtttagtctt gcccctaaaa tctcgccaca tttgtctaag
     2041 tgatataaat aggctttggt gtagtttttg caagtgtaac aatcacattc aggatcaagc
     2101 ggtgtggtgt ctgcacgata tttcgcatta cgaattttta caatgccgtt ggtcacaaat
     2161 aaatgtccgt tacgggcatt gcgggttggc attacgcagt caaacatatc aataccacgg
     2221 cgaacccctt cgactaaatc ttctggcttg cctacaccca ttaaatagcg tggtttatct
     2281 gccggaattt gcgggcaaac gtattctaaa atgcggtgca tatcttcttt aggttcacct
     2341 accgccaagc caccgactgc atagccatca aagccgattt caactaattt ttccaccgag
     2401 actttgcgaa gttcttcata aataccacct tgaataatgc caaataacgc attcgggttt
     2461 tcaagttcat caaagcgatc acgagaacgt ttcgcccaac gtagtgacat ttccatcgat
     2521 ttttttgcat aatcaaaggt tgccggataa ggagtacatt catcaaaaat cattacaatg
     2581 tctgatccaa gatcgtattg gatctccatt gatttttccg gcgaaaggaa aattttctca
     2641 ccgctaatcg ggttttggaa agttacgcct tcttctttaa ttttacgcag tttacctaag
     2701 ctaaaaactt ggaaaccgcc ggagtcggtt aaaatagggc cgtgccattg cataaaatcg
     2761 tgcaagtcac cgtgcatctt catcacttct tgccccggtc ttaaccataa atggaaagtg
     2821 ttgcctaata aaatttgcgc ccctgtcgct ttaacttctt caggagtcat tccttttacc
     2881 gtgccataag tgccaacagg cataaatgcc ggtgtttcaa cgtgaaattc gcctttcggg
     2941 cggttaaaag ttaaacgccc acgacgagcg ttaccacttg ttgtttttaa ttcgtacttc
     3001 attttaatcc acaaataaac agttcgtgaa agcggtcaaa aaccaccaaa aatttaccaa
     3061 ttagaatcga aagtcggtgc cgacccctaa gccgattcca acgccgttag agccaccacc
     3121 ggctccaata ctgccaccca caccggttga tgagcaagca gctaacacta aaatgccaac
     3181 gcataataag agttttttca ttttttgctg tcctaaacga aaaagagagt ctatgataga
     3241 cccaatgtat tctaacctgt ttaatggatt aaacctaatt ttaaagggta aattatgaaa
     3301 attttacgta aattcgctgc tgtatgcgtt gtctcaatgg ctatgctctc tgctaacgct
     3361 aatttgctca ctattagcga gcaggaaatc aacgactatt tagccaccaa attagctgaa
     3421 aaaattcctt taaaagataa agtcggtatt cctaaattat ttgaattgga ttatcgcctg
     3481 cattcactta ccagccaaat tggcagaacc gaggaaaaaa aagtagctat ttctggcgtt
     3541 gttgatggac ttttaaacgc aaaaggcaag aaatatgctg cctctatcca tttaaatatg
     3601 gataccacgc cttattatga tccacaaaaa ggggcattat ttcttaaaga tgtcagattg
     3661 ttaagttggt ctgcaacacc ggctaaatat caagcagagc tgcaaatgtt tttgccggtg
     3721 ttggctgatg gattaagtag ttttttaaac agtacgcctg tttatacttt agatgaaaat
     3781 aaaacaaaag aagcaatggt gaaaaagttc ggcaaagcca ttgtagtaga aaatggtaat
     3841 atcaagctag aaaccactat tttttaaata attttgtgat cttcgtctaa tttttacact
     3901 ttgatagttg agcttttacg cgttttattt acaatccaaa tatcgccatt cattatttgg
     3961 agagttctat ggatatcggt attgttaaat ggtttaacaa tgtaaaagga tttggtttta
     4021 ttacatcgga tgtatgtgaa ggagacattt ttgctcattt ttctgaaatt caagcagaag
     4081 gttatcgctc tttaaaagtc ggccaaaaag tacagtttga attagttcaa ggagagcgag
     4141 gtgcctcagc ctcaaatatc acgcctataa gcgaaaaaac gttataatcc gtcgttaata
     4201 aaattattgt tataaccata ctttattaga ttttaaagct cagcttttac agctgagcca
     4261 tttttgcttt ttgaatatgc cttcctctta tcaattagtc ttaattaacc tactttatat
     4321 cttcggtatt ggtctaaata ttgtactgct taggtttctc agtttgcatt ttgacccatt
     4381 aaataataac ggtatacgtt ttcttgttgg tgggttcacc ttattatttt tcattttatg
     4441 gagattccgc acatcgctgt ctcagctgat taaaactcca aaattgattg cgatttctct
     4501 atttgtcggg ttaatgatgt ccgcaaatat gtatttttgg ctaaaagggg tcaaaattac
     4561 aaatgctgtt accgtctcta tttttggggt attagctatg ccatttggtg tgattattgc
     4621 cgccatattc tttcaagatg aaagagaacg cattaaaagc aaagctttct ggttcggctc
     4681 aattttaacg atcatcggtt cactcggatt tatttggcac gggaaaacac tctcgattgg
     4741 cgaaggattt attcttggtt ctgtgttctt atttttgtcg atcattatcc gtaatataca
     4801 aaacctagtt gtaaaatttg ctcgagaact gaacgtatta accctaagtt gtgtaacatc
     4861 tttaactact tcactctcga gtctattact tagccaacaa gcaggtaaat ttaatgagct
     4921 ttttactgca ccaaactggc tactattctc tctgatggtt gctggtattt atgcgattat
     4981 gatcggaatg gtattaacct ttcacattat ccaaacccaa ggactgataa cctaccaagt
     5041 attggaacta ttaatgccgg tttcaacagc catcattgct tatattttac tggacgaacg
     5101 tatttccatt gttcaaatgg gctttgcttg tattgtgatt ttaggctcaa gtatcgcctt
     5161 aaagttagta aaatatccta agcactatta ggatctttgc taattaatat tggtaaaatt
     5221 acatttggtt tactaatctg agcgttcatt cattagtaat gataaacaag cggttatttt
     5281 ttgttatttt ttggtaaata ctgatagtca tgtataaaaa cctttctttg ctttcaccga
     5341 cttatccatt tgaccttgaa ttcatcgccg gtcttactca atgtgagaaa ggtaactatc
     5401 ttgattctat tgtcgatagg caacaaggaa tgcacggtta tatgcttcaa ttaaccactt
     5461 ttggtcgtgg ttctgttttt gatggtaagc acgtttttac cgctcatcga gggcagttac
     5521 tcctctttac acccaatgcc gttcaacact accaccgtca cccggaaagc caatattggc
     5581 actacaaatg ggtctatttt cagccgaatc ctaaatggaa taaatggctg aattggtcag
     5641 atatggaaga aaaaatagga cgtatcacca ttagcgatga tcgttacttt caggaaatta
     5701 gccaactttt cagtaaaatt gaattaga
//
LOCUS       DANLUQ010000145         5661 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.146, whole genome shotgun sequence.
ACCESSION   DANLUQ010000145 DANLUQ010000000
VERSION     DANLUQ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5661
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.146"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            298..1335
                     /gene="rsgA"
                     /locus_tag="PXB27_002142"
     CDS             298..1335
                     /gene="rsgA"
                     /locus_tag="PXB27_002142"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HDL5346889.1"
                     /translation="MSKPKLTHNQQRRIQSNHHKRINKKEIEWQDEMLGEMQQGIVVT
                     RHVKHADVETAEGQIFRCNIRRTLKNVVVGDQVSWRKGSEQLQGISGVIEAVFPRKNE
                     LTRPDYYDGIKVMASNIDQIIIVSAVLPTLSLNIIDRYLVICETANIPALIVLNKIDL
                     LNESERQAVKEQLKIYEEIGYETLCLSADTGENMQALHQYLAKGTSIFVGQSGVGKSS
                     LINQLLPEVNALTGSVSDNSGLGQHTTTASRLYHLPQGGQLIDSPGIREFGLWHLEPE
                     QITLGYREFQSVLGTCKFRDCKHKTDPGCALRQEVENGEISAIRFENYHRLIESLSET
                     KSQRHFRKEEV"
     gene            1332..1931
                     /gene="rnhB"
                     /locus_tag="PXB27_002143"
     CDS             1332..1931
                     /gene="rnhB"
                     /locus_tag="PXB27_002143"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005618717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDL5346890.1"
                     /translation="MMNEFIYPNARLIAGVDEVGRGPLVGAVVTAAVILDPNNPIQGL
                     TDSKKLSEKKRQALAEEIKAKALSWSLGRAEPTEIDELNILNATMLAMQRAVAGLHIQ
                     PDFVLIDGNRPPKLPMPAQAVVKGDSLVAEISAASIIAKVARDQEMDELDQQFPQYGF
                     AQHKGYPTKLHFEKLAEFGATPYHRKSFAPVRKVLGLID"
     gene            1944..2303
                     /locus_tag="PXB27_002144"
     CDS             1944..2303
                     /locus_tag="PXB27_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HDL5346891.1"
                     /translation="MTTEYQKTIFTKIINKEIPANIVYQDELVTAFRDIAPQAPTHIL
                     IIPNKFIPTVNHVSNEDELALGRLFTVAAKLAQEEGIAEEGYRLVMNCNKNGGQEVFH
                     IHMHLLGGQKLGPLVAK"
     gene            2306..2677
                     /locus_tag="PXB27_002145"
     CDS             2306..2677
                     /locus_tag="PXB27_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HDL5346892.1"
                     /translation="MRISYFAFAIFSIFLTACGSKPVSYLPTGSEPIVNIESDIAEKI
                     ELETNSKQFSTTNLTEFPLNLTYKLFWYNKDGVTQSHNGISDSIGWQHLWLQPKQKQT
                     IALIKPTEESHNYRLYLRGVK"
     gene            complement(2712..3341)
                     /locus_tag="PXB27_002146"
     CDS             complement(2712..3341)
                     /locus_tag="PXB27_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL5346893.1"
                     /translation="MFNLQIIPVSPFQQNCTVIWDENKNAAIIDGGDNAEGIIQFVES
                     QQLKVQKLLITHGHLDHIMAVEKLREHFQVEVFGSHLADKPLFERLPEMCQQFGLPPV
                     NGFLPDHWLEEGDRVEVGELSYTVRHLPGHAPGHIGFFDFDNRIAFSGDVLFQNSIGR
                     TDLYLGNHSQLLETIREKMFDLDDDFIIIPGHGPHTTIGREKQTNPFLK"
     gene            complement(3358..3912)
                     /locus_tag="PXB27_002147"
     CDS             complement(3358..3912)
                     /locus_tag="PXB27_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HDL5346894.1"
                     /translation="MKQINVQRRKWLSLGGLVLGASLLPGQVMAALSTPAPAALRFRN
                     VNTGDTYTAKFGAGGLNKTDLSQLNYLMRDRHINQVKAIDPKLFVKLNQLQRRLGFHN
                     AEILVLSGYRSAQTNARLRRRSRGVASHSYHILGQAVDFQVSGVPLYKVKQAAESLNN
                     GGVGYYPRSNFVHVDTGPVRTWRG"
     gene            complement(4082..5587)
                     /locus_tag="PXB27_002148"
     CDS             complement(4082..5587)
                     /locus_tag="PXB27_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010129962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HDL5346895.1"
                     /translation="MVAFSGMALAETATVAQVASEPVVQDNILPQLSFAAQQARAAEL
                     VKQADYALEQEQIRQEQARKFAEAEARIKQEIGNNALLLSQATTKVYLDSEFATMWND
                     KAAEKAFLKEYALFAVSGVSSKSAKALQQILNQPEGLGRDILLTDGFLDYLYYNKNVF
                     KNANNWLYNLGSYVPKSPSSEQIFQWTNAVREGDSGQFVTDLVPRNHLYRDTAQRVLA
                     MAGGSSAPKAKKGKAVTEVAGGGNGASFYKLALNAQRLRIIPSFNNGIFVNIPSYQLY
                     YFRDGNLALQSKVIVGREDRRTPVMYSKLSNVVVNPPWNVPTSIMTKDIIPKLARDPG
                     YADRASFEILDSSGNKINPRSVNWSQYLNAKSSPYRIRQKAGDDSALGRYKFNMPSSD
                     AIYLHDTPNRGLFGKTGRALSSGCVRVEKSDELATILLKEAGWGSDKKNQVLGSKKTT
                     SAQIRSDNPVYLYYVTAWVEGGKVQTLPDIYNFDAKIPQHSVNWGKLKSII"
BASE COUNT         1743 a         1212 c         1064 g         1642 t
ORIGIN      
        1 aaattttaag tatgatgttg tgtttgcata ttggtctagg aaactagact agagtaacga
       61 aagttacttg tttcacttcc tgtatatttt gaaccgtatt ggtttataac cgcccacttt
      121 gggcggtttt tttctatgta aaaaaacagc acaaaaccta gatcgcattc tatattattc
      181 gctaccttta ttctagcctt tctgcttttt tattctttca ctttaatcta ttataaaaag
      241 aggttttata gtatgctaaa gcctattttt catttgcaat aattaccgag agttcttttg
      301 agtaaaccga aattaaccca taaccaacag cgtagaatcc aatctaacca tcataagcgt
      361 atcaacaaaa aagagattga atggcaagat gaaatgttag gagaaatgca acagggcatc
      421 gtcgttactc gccacgttaa acacgctgat gttgaaaccg cagaggggca aattttccgc
      481 tgtaatatcc gccgtacatt gaaaaatgta gtggtgggcg atcaggtttc ttggcgtaaa
      541 ggtagtgagc aactacaagg gattagtggg gtcattgaag ccgtcttccc acgtaaaaat
      601 gaacttaccc gccccgatta ttatgacggt attaaggtga tggcatcgaa catcgatcaa
      661 atcattattg tatcagctgt gctacctaca ctttcgctga atattatcga ccgttattta
      721 gtgatttgcg aaacggcgaa tattcctgcc ttaatcgtgc taaacaaaat tgatttatta
      781 aacgaaagcg aacggcaagc agttaaagag cagctcaaaa tctatgaaga gattggctat
      841 gaaaccctat gtttatcggc tgatacgggt gaaaatatgc aggcgttaca ccaatatctt
      901 gctaaaggta cgtctatttt tgtcggacaa tccggcgtgg ggaaatccag cctaattaac
      961 caactgttac ctgaagtcaa tgctctcact ggctcagtga gcgataactc agggttaggt
     1021 cagcacacca ccactgcctc tcgcttatac catttaccgc aaggcggaca gctgattgac
     1081 tcgccgggca ttcgagaatt tggcttatgg catttagaac ctgagcaaat caccttaggc
     1141 tatcgagaat ttcaatcggt tttaggcacg tgcaaattcc gtgattgcaa acacaaaacc
     1201 gacccgggtt gtgccttaag acaagaggtc gaaaatggcg aaatttctgc aattcggttt
     1261 gaaaattacc atcgcttaat tgaaagcctg tctgaaacca aaagccaacg ccatttcaga
     1321 aaagaagaag tataatgaat gaatttattt accctaacgc ccgattaatc gccggtgttg
     1381 acgaagtcgg acgaggacct ttagtcggag cggttgtgac cgcagccgtg attttagatc
     1441 cgaataaccc gattcagggc ttaaccgatt ccaaaaaatt atccgagaaa aaacgccaag
     1501 ctttggcaga agagattaaa gccaaggctc tgagctggtc gctaggacgt gccgagccga
     1561 cagaaattga tgagctgaat attttaaatg cgacaatgtt ggcaatgcaa cgtgcggttg
     1621 ccggcttgca tattcagccg gattttgtgc taattgacgg caatcgtccg ccaaaactgc
     1681 caatgccggc acaagcggtg gtgaaaggag acagcttagt ggctgaaatc agtgctgctt
     1741 cgattattgc caaagtcgcc cgagatcaag aaatggacga attagaccaa caatttccgc
     1801 aatacggctt cgcacagcac aaaggctacc cgactaaact ccattttgaa aaattagcag
     1861 aatttggggc aacaccttat catcgtaaga gttttgcccc tgtaagaaaa gtattaggtt
     1921 taatcgatta gaaggaaaac acaatgacta cagagtacca aaaaacgatt ttcaccaaaa
     1981 tcatcaataa agaaattccg gcaaatattg tctatcaaga tgaacttgtg accgcatttc
     2041 gtgatattgc tccacaagca cccacgcata ttttgattat tccgaataaa tttattccaa
     2101 ccgtcaatca cgtgagtaat gaagatgaat tagccctagg gcgtttattt acggtggcgg
     2161 caaaattagc ccaagaagaa ggcattgccg aagaaggcta tcgcttagtg atgaattgta
     2221 ataaaaacgg cgggcaggaa gtgttccata ttcatatgca cttgttaggc ggacaaaaac
     2281 taggaccttt agtggcaaaa taactatgcg tatttcctac tttgcatttg caattttttc
     2341 gatttttttg accgcttgtg gtagcaaacc tgtcagctat ttaccaaccg gctcagagcc
     2401 tattgtcaat attgagtctg acattgctga aaagatagag ctggaaacga atagcaagca
     2461 atttagcacc acaaatttaa ctgaatttcc gcttaatctt acttataaat tattttggta
     2521 taataaagat ggggtaacac aatcccataa cggtatatca gatagtatcg gctggcaaca
     2581 tctatggttg caacctaagc aaaaacagac cattgcactc attaaaccga cggaagaaag
     2641 ccataattac cgcctctatt tacgaggcgt aaaatagcaa aaaagcagag ccaactgctc
     2701 tgcttcttta tttatttcaa aaacgggttg gtctgttttt cccgcccgat agttgtgtgt
     2761 ggaccatgtc ccggtatgat gatgaaatca tcatctaaat caaacatttt ttccctgatc
     2821 gtttccaata actggctatg gttgcctaaa tagagatctg ttcgacctat gctattttgg
     2881 aataaaacat cgccactaaa agcaatacga ttgtcaaaat caaaaaaacc gatatgaccc
     2941 ggagcatgac ccggcaaatg tcgaacggta tagctcagct cgccgacttc gacccgatca
     3001 ccttcctcca accaatgatc cggcaaaaag ccatttactg gtggtaatcc aaattgttgg
     3061 cacatttccg gcaaacgttc aaataaaggt ttatctgcaa gatgcgagcc gaaaacctct
     3121 acttgaaaat gctctctcaa cttttccacc gccataatat gatctaaatg accgtgcgta
     3181 atcaataatt tctgaacctt aagctgctga gattcaacaa attgaataat gccttcggca
     3241 ttatcgccgc catcaataat agccgcattc ttattttcgt cccaaatcac cgtgcaattt
     3301 tgttgaaatg gactaacagg aataatttgc aaattaaaca taaaataaga ccgcttgtta
     3361 gcctctccaa gtgcgaactg ggccagtatc tacgtgaaca aagttactac gaggataata
     3421 acctacgcca ccattattca agctctcagc cgcttgtttt actttatata acggcacacc
     3481 tgatacttgg aaatctactg cttgcccaag aatatggtaa ctatggctgg caacaccacg
     3541 gcttctacga cgaagtcttg catttgtttg tgcggagcga tagccactta atactaggat
     3601 ttccgcatta tggaaaccta aacggcgttg taattgattc aatttgacaa ataattttgg
     3661 atcaattgct ttaacttgat tgatatgacg atcccgcatt aaatagttta actggcttaa
     3721 atcggtttta tttaatccac ctgcaccaaa tttagcagtg taagtatcgc ctgtattcac
     3781 attacgaaaa cgtaacgcag ctggggcagg agtagaaagc gctgccatta cttgacccgg
     3841 cagaaggcta gcccctaaaa caagtccgcc gagtgatagc cacttacggc gttgaacatt
     3901 aatttgtttc attttttcct cataggttag ctaatgattt atagaaaatc actcacaaat
     3961 gagcgaattt ctatacacca tacttaaagt aaaaatttac aatcgtaaat ttatgtaagc
     4021 tatattttca aaaagttcca agaagatgat atcttcttgg aatttatcag aaatagcacg
     4081 attaaataat gctctttaat tttccccaat tcacagaatg ttgtgggatt ttggcatcaa
     4141 agttataaat gtccggaagg gtttgcactt ttccaccttc aacccaagcc gtcacataat
     4201 agagataaac cgggttatct gaacgaatct gagccgaggt cgtcttctta ctgcctaaga
     4261 cttgattctt tttatcagat ccccaacccg cttcttttaa taaaatggtt gccaattcat
     4321 cagatttttc tacacggaca caaccagaac ttaacgcacg ccccgtttta ccaaataatc
     4381 ctctatttgg cgtatcatgc aaataaattg catccgaact tggcatatta aatttataac
     4441 gtcctaaagc gctatcatca cctgctttct ggcgaatacg gtatggtgaa cttttagcat
     4501 ttaaatattg tgaccaattt acgctacgcg gattaatttt attgccacta ctatcaagaa
     4561 tttcgaaact agcacgatct gcataacctg ggtcacgcgc tagtttagga ataatatctt
     4621 tagtcataat actagtcggt acattccaag gtggattcac taccacattg ctcagcttac
     4681 tatacattac cggtgtacgg cgatcttctc gcccaacaat cactttagat tgaagagcta
     4741 aattaccatc acggaaataa tataattgat agcttgggat attcacaaaa attccattgt
     4801 taaaacttgg aataatacgt aaacgttgag cattgagcgc taatttatag aaagaagccc
     4861 cattaccgcc accagctacc tccgtaacag ctttaccttt tttggcttta ggtgctgaag
     4921 agcctcctgc cattgccaat actctctgtg ccgtatcacg atataaatgg tttcttggaa
     4981 caaggtctgt tacaaactgc ccagaatcgc cttccctcac ggcattagtc cattggaaga
     5041 tttgctctga agagggcgat tttggtacat aactgcctaa gttatataac caattatttg
     5101 catttttaaa cacatttttg ttgtaataca aataatctaa gaagccatca gttaataaaa
     5161 tatctctacc taaaccttcc ggttgattta agatttgctg caatgcctta gctgatttag
     5221 aggatacccc acttactgca aataatgcat attctttcaa aaatgctttt tctgcggctt
     5281 tatcattcca cattgtcgca aattcactgt ctaaataaac cttagttgtt gcttggctca
     5341 gcaataaggc attattacct atttcttgct taattcttgc ttccgcttct gcaaattttc
     5401 ttgcttgctc ttgacgaatt tgttcctgtt ctaacgcata atcggcctgt tttaccaatt
     5461 ctgcagcacg agcctgctga gctgcaaaac ttaattgtgg taaaatatta tcttgtacaa
     5521 caggctcaga agccacctga gcaactgtgg ctgtttcagc caaagccatg ccagaaaacg
     5581 ccaccataat aggtaataat ttaaaggttt tcacccactc ttcctcttaa tatttgcaaa
     5641 tttataaaaa aataaaccgc t
//
LOCUS       DANLUQ010000146         5604 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.147, whole genome shotgun sequence.
ACCESSION   DANLUQ010000146 DANLUQ010000000
VERSION     DANLUQ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5604
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.147"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(315..1058)
                     /locus_tag="PXB27_002149"
     CDS             complement(315..1058)
                     /locus_tag="PXB27_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346896.1"
                     /translation="MLNKHINKIINSSFSLAFFTLTSFATAYCYGWGQAIFHGYPWWH
                     VEIGNASMARSLAYVFGTSVILFLCYALGYSLLNKVFQLHYFRNLGWLRVIVLVSVFT
                     VPVILTFYLFTGIVPIYISFIYIFVSGVSVFFFRKRWNNTKFDLDFRKMLLGGSFALF
                     NIFIFLYFSLLSLHIGYLRAGLRTTYDYIEIENQKYYILSTNSDNGYIMGTKIRGNQS
                     FLFFNRDTHQYYHVHIEQCTDSRLCKVIV"
     gene            complement(1229..1567)
                     /locus_tag="PXB27_002150"
     CDS             complement(1229..1567)
                     /locus_tag="PXB27_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL5346897.1"
                     /translation="MKLITTIIQPSKLDQVQDALSEIGIQGMTITEVKGFGRQYGHTE
                     QELSQMSRIDFRPKIKVEILVSNYQTDEVIDAILNASYSGQVGDGKIYVANIEQVIRT
                     RTGETDDEAV"
     gene            1826..3724
                     /gene="dnaK"
                     /locus_tag="PXB27_002151"
     CDS             1826..3724
                     /gene="dnaK"
                     /locus_tag="PXB27_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDL5346898.1"
                     /translation="MGKIIGIDLGTTNSCVAVMDGDKPRVIENAEGARTTPSIIAYTD
                     KETLVGQPAKRQAITNPKNTLFAIKRLIGRRFTDAEVQRDIEIMPFEISKADNGDAWV
                     TVKGDKLAPPQISAEILKKMKKTAEDFLGEPVTEAVITVPAYFNDAQRQATKDAGRIA
                     GLDVKRIINEPTAAALAYGLDSKKENQTIAVYDLGGGTFDISIIEIDNFDGEQTFEVR
                     ATNGDTHLGGEDFDNRVINYLVEEFQKEQGVDLRNDPMAMQRVKEAAEKAKIELSSAQ
                     ETEVNLPYITADATGPKHLNIKVTRAKLESLVEDLVNRSLEPLKTALADAGLSVGDIN
                     DVILVGGQTRMPLVQKKVADFFGKEPRKDVNPDEAVAIGAAVQGGVLSGSVTDVLLLD
                     VTPLSLGIETMGGVMTTLIEKNTTIPTKKSQVFSTAEDNQSAVTIHVLQGERKRAADN
                     KSLGQFNLEGINPAPRGMPQIEVTFDIDANGIINVSAKDKNTGKEQQIKIQASSGLSD
                     AEVEQMVRDAEANAEADKKFEELVQARNQADGIAHATRKQITEAGDALNADDKAKIEA
                     AISELETAAKGEDKAEIEAKIEALIKASEPLMQAAQAKAQGGEQPQQSQKDDGVVDAE
                     FEEVKDNK"
     gene            3866..4168
                     /locus_tag="PXB27_002152"
     CDS             3866..4168
                     /locus_tag="PXB27_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346899.1"
                     /translation="MLKLYPTTENVQPPFYKIYDHKSGETVIATETENSNTAILMDDF
                     VWFEQIPTTEALLADTWSVIENRSKQGLLKRAYTKDFEKHLADGKVEIYIAVNPHC"
     gene            4391..5530
                     /gene="dnaJ"
                     /locus_tag="PXB27_002153"
     CDS             4391..5530
                     /gene="dnaJ"
                     /locus_tag="PXB27_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015939959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDL5346900.1"
                     /translation="MAKKDYYEVLGLSKGASEKDIKRAYKRLAAKHHPDKNQGSKESE
                     EKFKEITEAYDVLTDSEKKAMYDQYGHAAFEQGGAGAGGFGGFGGGGFGGFEDIFSEM
                     FGGGFGGGGRRQRVVRGDDLRYDIEITLEEAVKGCKKDIRIQTLAECDTCHGTGAEAG
                     SKVETCSHCHGSGRVRRQQGFFVTEAVCPSCHGTGKRIEKPCRSCHGDGRVQKAKNLS
                     VTIPAGVDTGNQLRLSREGAAGENGAPAGDLYVVIHVKEHDIFERDGSNLYCEVPISF
                     TLAALGGEIEVPTLDGKLKLKIPAETQTGKLFRVRGKGVTSPRGGYAGDLICKVVVET
                     PVNLTEEQKALLRQFEESLEGQGKHRPKHEGFFDGVKKFFDNLGK"
BASE COUNT         1816 a         1099 c         1210 g         1479 t
ORIGIN      
        1 atcgagaatt atgttctttt ttattttgtt attctcttta ggtataaaaa aacccggtaa
       61 ataccgggct tttatttcaa ttggttatcc aacttgcgag agcaattacc gcaataaaaa
      121 taactaacca aataagttgt aattttacgc ttttgcggtt gatctcctgg ttgtgtaatc
      181 ggcgctgttc taacttttgc cgatatatcg ctaaatcggc ttctttaaac gagaagccac
      241 aatgtatgca atgttctaaa ctgtcgctaa tacgctttcg acactccgga caacggtgta
      301 atgccataat tacctcaaac tattactttg cataatcggc tatccgtaca ttgttcaata
      361 tgaacatggt agtattgatg agtatcacga ttgaaaaaca agaaagattg attacctctt
      421 atttttgttc ccataatgta gccgttatca ctgttggtgg ataatatgta gtatttttga
      481 ttctctattt cgatgtaatc ataggtagtt cttagtccgg cacgcaaata accaatatgc
      541 aatgagagca aactgaaata gagaaaaata aagatgttaa acagggcaaa acttcctcct
      601 aatagcatct ttctaaagtc cagatcaaac ttggtattat tccatctctt gcggaagaag
      661 aatacgctca caccacttac aaaaatatag ataaaagaga tataaatggg cactatcccg
      721 gtaaataagt aaaaagtaag aataacaggc actgtaaaca cagaaaccaa cacaatcacc
      781 cttaaccaac ctagatttcg gaaataatgc agctgaaaaa ctttatttag taaactataa
      841 cctagtgcat aacacaagaa taagatgacg gaagtaccga atacataagc caaacttctt
      901 gccatactgg cattgccgat ttcaacatgc caccaagggt agccatgaaa tatggcttgt
      961 ccccaaccgt aacagtaagc ggtagcaaaa ctagttaatg taaaaaatgc aagagaaaat
     1021 gaagaattga taattttatt aatgtgttta tttagcataa caaagaatgt ctatttccat
     1081 taaaacctaa ctatttttga ttactataat aacccgtatt acacaaaaaa caaacattta
     1141 attcacacca aattagaaaa tttgctttag taaggttgaa aatataactc taaaacacga
     1201 taataaaaag cttctctgct agcaatggct agactgcttc atcgtcagtt tcgccggtac
     1261 gggtgcggat aacttgttca atattcgcta cataaatttt gccatcaccc acttgcccag
     1321 aataggatgc attgagaatg gcatcgatca cttcatcggt ttggtaatta gaaaccaata
     1381 tctccacctt aattttagga cgaaagtcaa tgcggctcat ttggctaagc tcttgttcgg
     1441 tatgcccata ttggcgtcca aagcctttta cttcggtaat agtcattcct tgaataccga
     1501 tttctgaaag tgcatcttgc acttggtcta attttgaagg ttggataatg gtagtaatca
     1561 gtttcatatt atatccttct ctgtttgagt tgccgtaagt ataaaaagct attgaggggt
     1621 atgcaaggta ggaggtattt tctccgtgaa atattctgcc aaatctcccc cacatacaag
     1681 cggttacttt ttccaaaaat tttgcaattt ttttattttt gcccttgaac agtgcatttt
     1741 cgtccctatt tatgatttcg tctgaacggg aaatgcaatc ccgtttctta ttcaaactga
     1801 atttattatt ataggaatca taaaaatggg taaaatcatt ggtattgact taggtacaac
     1861 aaattcttgt gttgcggtaa tggacggcga caaacctcgt gtaattgaaa atgcggaagg
     1921 ggcaagaaca acgccatcta tcatcgctta caccgataaa gaaaccttag ttggtcagcc
     1981 ggcaaaacgt caggcaatta ctaacccgaa aaatacttta tttgcaatca agcgtttaat
     2041 cggccgtcgt tttactgatg ctgaagtgca acgtgatatt gaaattatgc cgtttgaaat
     2101 cagcaaagca gataacggcg atgcgtgggt aaccgtaaaa ggcgataaat tagcaccgcc
     2161 gcaaatctct gctgaaatct tgaaaaaaat gaagaaaacc gcagaagatt tcttgggcga
     2221 accggtaacc gaagcggtta ttactgtgcc tgcatacttt aacgatgcac aacgtcaggc
     2281 aactaaagat gccggtcgta ttgcaggttt agatgtaaaa cgtatcatca acgaaccaac
     2341 agcggcagcg cttgcttatg gtttagattc taagaaagag aaccaaacga ttgctgttta
     2401 cgacttaggt ggtggtacat tcgatatttc gattatcgaa attgataact ttgacggcga
     2461 gcaaactttt gaagtgcgtg caaccaacgg cgatactcac ctaggtggtg aagacttcga
     2521 taaccgtgta atcaactact tagtggaaga attccaaaaa gaacaaggcg ttgatttacg
     2581 caacgaccca atggcaatgc aacgtgtgaa agaagcagcg gaaaaagcga aaatcgaact
     2641 ttcttctgca caagaaactg aagtgaacct tccatacatc acggcagatg caaccggccc
     2701 gaaacactta aacattaaag taacccgtgc gaaattggaa tctttagttg aagatttagt
     2761 aaaccgttcg ttagagccgt taaaaaccgc tttagctgat gccggtttat cagtaggcga
     2821 catcaacgat gtgattttag tcggtggtca aacccgtatg ccgttagtac agaaaaaagt
     2881 ggcagacttc tttggcaaag aaccgcgtaa agacgttaac ccggacgaag ctgtggcaat
     2941 cggtgcggcg gttcaaggcg gtgtgttaag tggttcggta acagacgtat tgttattaga
     3001 cgttactccg ctttcacttg gtatcgaaac aatgggtggt gtaatgacta ccttaattga
     3061 gaaaaacact acgattccaa ccaagaaatc acaagtgttc tcaacagcgg aagataacca
     3121 atctgcggta actatccacg tgttacaagg tgagcgtaaa cgtgcagcgg ataataaatc
     3181 tttaggtcaa ttcaaccttg aaggtattaa ccctgctcca cgcggtatgc cacaaattga
     3241 agtaaccttt gatattgatg ctaacggtat tatcaatgtc tctgcaaaag ataaaaacac
     3301 tggcaaagag caacaaatca aaattcaagc atcttcaggc ttaagcgatg ctgaagttga
     3361 acaaatggtg cgtgatgcag aagcgaatgc ggaagcagac aaaaaatttg aagagttagt
     3421 tcaagctcgt aaccaagcag acggtattgc tcacgcaacc cgtaagcaaa ttaccgaagc
     3481 aggcgatgcg ttaaacgcag acgataaagc gaagatcgaa gcggctattt ctgagttaga
     3541 aaccgcagct aaaggcgaag ataaagccga gatcgaagcg aaaatcgaag cgttaatcaa
     3601 agcttcagaa ccgttaatgc aagcagcaca agcaaaagca caaggcggcg agcaaccaca
     3661 acaatctcaa aaagatgacg gcgtggtcga tgcggaattt gaagaagtga aagataataa
     3721 ataatcttgc gtaacttgta ggggcgaaat atctttcgcc ccttttgtgt agatatatac
     3781 cacctagcac gagccgtgct aggttacaca taattcagcc cctttggggc tgtgcaagcg
     3841 gtgcttttca aaggaaattt tacaaatgct aaaactctac cctacaacgg aaaatgtaca
     3901 gccaccgttt tataaaatat acgatcataa aagtggcgaa acggtcattg ccacagaaac
     3961 ggaaaacagc aataccgcga ttttaatgga cgattttgtt tggtttgagc aaattccaac
     4021 taccgaagct cttttggcag acacttggtc tgtgattgaa aaccgttcca aacaaggttt
     4081 gctcaaacga gcttacacca aagattttga aaaacattta gcagacggca aagtagaaat
     4141 ttatattgcg gtgaatccgc attgttaggc ttttgatagc gcaagcgtcc acgcttgtgt
     4201 catcttgagc catcttggct agatcccatt taattatata aaactctctc cccttgtggg
     4261 agagagaccg aaaaatattt cgttcagaaa tatttttcag agagaggggg aattaaccat
     4321 cctacccctc tcccacaagg ggcgagggca agcggtcaaa aacacagaaa aatttacaaa
     4381 cggaacaaca atggcaaaga aagattatta cgaagtcctt ggactgtcta aaggggcaag
     4441 cgagaaagac atcaaacgag catataaacg tttggcggca aagcatcacc cggataaaaa
     4501 ccaaggcagt aaagagtcgg aagagaaatt taaagagatt accgaagcct atgacgtgct
     4561 gaccgacagc gagaaaaaag cgatgtacga ccaatacggt cacgccgcct ttgaacaagg
     4621 cggtgccggt gcaggcggat tcggtggctt tggcggcggt ggtttcggtg gttttgaaga
     4681 catcttcagc gaaatgtttg gcggtggctt tggtggcggt ggacgtcgcc aacgtgtggt
     4741 acgtggcgat gatttacgtt acgacatcga gatcaccctt gaagaagctg tgaaaggctg
     4801 taaaaaagat attcgcattc aaaccttagc tgaatgtgat acttgtcatg gtacaggggc
     4861 ggaagcaggg agcaaagtgg aaacctgttc gcattgtcac ggctccggca gagttcgccg
     4921 tcagcaaggt ttctttgtga ctgaggcagt ttgtccaagt tgtcacggta caggtaaacg
     4981 cattgaaaaa ccgtgccgtt cttgccacgg cgatggacgt gtacagaaag cgaaaaacct
     5041 ctctgttacc attccggcag gtgtggatac cggtaatcaa cttcgcttat cgagagaggg
     5101 agctgcaggc gaaaatggcg caccggcagg cgatttatat gtggtgattc acgttaaaga
     5161 acacgatatt tttgagcgtg acggttcgaa tttatactgt gaagtgccga ttagctttac
     5221 ccttgcagct ttaggcggtg aaattgaagt gccaacttta gacggcaaat taaaactcaa
     5281 aattccggca gaaactcaaa ccggtaaact attccgtgta cgaggcaaag gggtaacaag
     5341 ccctcgtggt ggctacgcag gggatttaat ctgcaaagtg gtggtcgaaa cgcctgtgaa
     5401 tttaaccgaa gaacagaaag cactattacg acaatttgaa gagagtttag aaggacaagg
     5461 taaacatcgt ccgaaacacg aaggtttctt tgatggtgtg aagaaattct ttgataactt
     5521 aggcaaataa aatcataaaa agtacatatt tcttaaatat gtactttttt attatctatt
     5581 ctttgttgaa atctacttct cggc
//
LOCUS       DANLUQ010000147         5554 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.148, whole genome shotgun sequence.
ACCESSION   DANLUQ010000147 DANLUQ010000000
VERSION     DANLUQ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5554
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.148"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            234..863
                     /locus_tag="PXB27_002154"
     CDS             234..863
                     /locus_tag="PXB27_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDL5346901.1"
                     /translation="MITVKFYGHLKTFGTDFKILVKDIAEAIRALCSQLKGLREALRD
                     GVYKVRIGKQYLDPSALEKGLFYCLKKGQTIHFTPVVKGAKSGGIFQAVLGVALIGAA
                     FALGPIGLGLVGSATAMTVGAMGASMLLGGVSQMLTKMPKAPTMGNETEKESSTAFSN
                     LNNLVAQGKPVPLAYGLIRTGSLVISQGVETITIKENQPASNKKTGFRK"
     gene            873..4604
                     /locus_tag="PXB27_002155"
     CDS             873..4604
                     /locus_tag="PXB27_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HDL5346902.1"
                     /translation="MRIYGAKGGGGSSHTPIEAPETGRSKQIVNIVELLCEGEIEGLV
                     DGFKSIYLDGTKIQNDDGTYNFNNVSGQLNVGTQDQDVLDGYDSSQNEVNVGVEIKKK
                     NGAIVRTVTDERINRLRLTLGVRSLFHQNNQGDTNTTNVDLKITIGTRQYSHSFNGKY
                     SSQYLESVVFDNLPPVPFNISVERLTEDSNSQRLQNGTIWSSYTEIIDTEFTYPNSAV
                     AGISFDSEYFNNIPTRNYLIKAKKVKVPSNYDPVKRTYTGFWDGTFKVAWTNNPAWEI
                     YDLAPILSKMLGVEISFDKWALYDVARYCDQLVPDGMGGMEPRFTCNVWLTEVKTAYD
                     LLNDFCSVFRAIPIWTGTEVSVIIDRPRDPVWTYTNANVVGGFERSYSARKSRHNAVQ
                     VTYSDKTNGYESAIEYVSDDEEIKKHGLNLSQITAFGCTSRGQAHRTGKWILETEKRE
                     KETITFTVGREGLMHLPGDIIRVADSHYAGTEIGGRVLAINGRKVTLDREISIDNASY
                     FTYINGEATHSSKIQSVNGKEITLDSTPTGLETYGVWSLSTKQISSGLYRSISIVENA
                     DGTNTITALQHEPQKEAIVDNSAHFVETARTLYKAPQINAVEVSTGYDGKLYITGDIS
                     SGDGKLTYDIKITKDGNLYQYKKGLSDPNIELSDLPNGDYTVIIYGKNAKGQIVTEKT
                     QTFTIDRPPAPTGVVVTGGLGQITLEWDWVNEVTQTEIFAAETDNFALAKKIAKVTAR
                     TYAHTLKGNKVVRYYWLRHTRGINVGPFYQQQGVRGQTAVDLDARLTELNTQLSRNIV
                     NEVFDVAAPARGLELVKTVANLTDKGTKLASSQVYNQADGKLYTWNGTAYSATVAAED
                     VTGKLSKSKIDTSLISQLTGADNTANLARRLAETAQSNINQEITNRQDAVTAEANNRT
                     KAIQAESANLTKKIQAEATARGAAVTQLQNVDAQQAQLISAVTAKADNALSGLEEEKT
                     ARANGDKAESQAREALTARMGAAESNIVTIQRTVANNAQSISEVSQNLNAKIDNINIG
                     GRNLLRDSEFNAYNKWGSPQIEFAENANRRTIKVTSTGTNGPVGIVSSNRHSTSYFQQ
                     GETYTLSLFARGSKALDYLYLMRQDGNNVRLPVINVASETEFNHYKLTFKAPFTTQQG
                     YVLIGFRQTSTEQFVEFHSVKLEKGNVATDWTPAPEDVESAVSAVSADLTSYKQTQAA
                     TDSAQAQQLNQLSVNLTKAETNFNAKITEEKKLVLMQIK"
     gene            4601..>5554
                     /locus_tag="PXB27_002156"
     CDS             4601..>5554
                     /locus_tag="PXB27_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5346903.1"
                     /translation="MNADKLTDITSRVANAESTITNFQSTKANKSEVASIAQQNLQSI
                     WRTDAQSAVDALKIGGANLLVDSEYLTTARWGGSSRVASSQYGDRRLTQVFVTQAGTG
                     HFGVTQGTQKATTRIRQGETYTLSLNAQGTAGFTRTGLNYVYLIREDGGNFRLPTLPL
                     TASLSQRPKVTFTAPWTSNQVRLLIGANGIFEATDWFAFHSVKLEMGNVATGWTPTAK
                     DIDDKVSAVQSNLTAYQAAQAKADQAKATQISGLTTRMGAAESNLTRTERAVTELNQT
                     TVTTLRDLTARTKTTEGSLSRLETAKASKTEVASIAQSSLQS"
BASE COUNT         1740 a         1160 c         1276 g         1378 t
ORIGIN      
        1 aagcgtttgc agcacttcgg ggtttttcat atccacttac ctaactaaga ggacataaat
       61 tgaatgaatg ataaacgatt tacattcaaa tttctaggag ttcttatgga agcaataaat
      121 attaccccaa aagaaatcag aaaaacaatg tggacagccg caataattct ctttttgttt
      181 gcgttcatct ggaaagcccc tgaatttatc actgctattc gttggtggta actatgatta
      241 cagttaaatt ttacggacac cttaaaacat tcggcacaga ttttaaaatt ttagttaaag
      301 acatcgctga ggcaattcgt gctctgtgtt cacagcttaa aggattgcgt gaagcgttgc
      361 gagacggtgt ttataaagtc cgtatcggta agcagtattt agacccatca gcccttgaaa
      421 aagggctttt ttattgcctg aagaaagggc aaaccattca ttttacccct gttgtcaaag
      481 gagcgaaaag cggtggtatt tttcaagcgg tgttaggtgt ggcgttaatt ggggctgcat
      541 ttgcgttagg tcctatcggc ttaggattgg ttggctctgc tacagcaatg acagttggag
      601 caatgggggc atcaatgtta ctaggaggag tttcccaaat gctcaccaaa atgccaaaag
      661 cccccacaat gggtaatgaa acagaaaaag aaagctcaac ggctttttca aatttaaata
      721 acttggtggc tcaaggcaaa cctgtgccgt tggcttatgg tttgattcgt accggctcgc
      781 tggtgatatc gcagggagtt gagacgatca ccattaaaga aaaccaaccg gcaagcaaca
      841 agaaaaccgg ctttagaaaa taagggataa ttatgcgtat ttacggagca aaaggtggtg
      901 gcggcagtag ccataccccg attgaagcac cggaaaccgg tcgctcaaaa cagattgtga
      961 atattgtgga attgctgtgc gagggcgaaa ttgaaggctt ggtagatggc tttaaatcaa
     1021 tttatcttga cggcacgaaa atccaaaacg atgacggcac ctataatttt aataatgtga
     1081 gcggtcagct aaatgtcggt acgcaagatc aagatgtgtt ggacggttac gatagctcac
     1141 agaatgaagt gaatgtgggt gttgagatta agaaaaagaa tggggctatt gtccgcaccg
     1201 taactgatga acgaattaac cgcttgcgtt taacgttggg agtaaggtca ttattccacc
     1261 aaaataatca aggcgatacc aataccacca atgttgattt aaaaatcacg attggcacac
     1321 ggcaatattc gcatagcttt aacggcaaat acagctcgca ataccttgaa tctgtggtgt
     1381 ttgataacct gccgcctgtg ccatttaata tctcagttga gcgtctaacc gaggatagca
     1441 attcgcaacg cttacaaaac ggtacaattt ggagtagtta caccgaaatt atcgataccg
     1501 agtttaccta ccctaattca gcggttgccg gtatcagttt tgattcggag tattttaata
     1561 atatcccgac ccgaaactac ctaattaaag cgaaaaaagt caaagtgcca agcaattatg
     1621 atccagttaa acgcacttac accggctttt gggacggcac atttaaagtt gcgtggacga
     1681 acaaccctgc ttgggaaatt tacgatttag ctccgatttt aagcaaaatg cttggggttg
     1741 aaatcagttt tgacaaatgg gcgttgtatg atgttgctcg ctattgcgat caacttgtgc
     1801 cggacggtat gggtggaatg gagcctcgtt tcacctgtaa cgtatggctc acggaagtta
     1861 aaactgccta tgacttgttg aatgatttct gctcggtatt ccgtgcgatt ccgatttgga
     1921 ccggaacaga agtatcggtc attatcgacc gtccgcgtga tccggtttgg acttacacaa
     1981 atgctaatgt agttggtgga tttgaacgat cctactctgc tcgcaaatca cgccataatg
     2041 cagtgcaagt tacttattcg gataaaacaa atggctatga aagtgcgatt gaatatgtct
     2101 ctgatgacga ggaaatcaaa aagcacggct taaatttaag tcaaattact gctttcggtt
     2161 gtacctctcg ggggcaagca caccgtaccg gaaaatggat tttggaaacc gaaaaacggg
     2221 aaaaggaaac cattactttt accgtgggac gtgagggctt aatgcacttg ccgggcgata
     2281 ttattcgtgt ggcagacagc cattatgccg gtacggaaat tggcggacgt gtactggcga
     2341 ttaatgggcg aaaagtcacg ttagatcgtg aaattagcat tgataacgcc agttatttta
     2401 cctatatcaa cggtgaggca acgcactcta gcaaaatcca atcggtaaac ggcaaagaaa
     2461 ttacgctgga tagtacacca accggcttag aaacctacgg tgtttggtcg ttatcgacta
     2521 aacaaatcag ctctggatta tatcgctcaa tctcgattgt cgaaaatgcg gacggcacaa
     2581 acactatcac agcgttacaa cacgaaccgc agaaagaggc aattgttgat aactccgctc
     2641 actttgtgga aacggctaga acactctaca aagctccaca aattaacgca gtggaagtct
     2701 cgaccggtta tgacggtaag ctctacatta ccggtgatat tagctccggt gatggcaagc
     2761 taacctatga tattaaaatt accaaagatg gcaatttata tcaatacaaa aaaggcttaa
     2821 gcgatccgaa cattgaacta agtgatttac ctaacggcga ttataccgtc attatttacg
     2881 gcaaaaacgc caaagggcaa atcgtcaccg aaaaaacaca aacctttact attgatcgac
     2941 ctccagctcc gaccggtgta gtagtaacag gtggattagg gcaaattacc ctcgaatggg
     3001 attgggtaaa tgaggttacg caaaccgaga ttttcgcggc cgaaaccgat aatttcgccc
     3061 ttgcaaaaaa aatcgcaaaa gtgaccgcta gaacctacgc tcacacacta aaaggtaaca
     3121 aggttgtacg ctattattgg ctacgtcata ccagaggcat taatgtcgga ccgttttatc
     3181 aacaacaggg ggtaagagga caaacagcgg ttgatttgga tgctcgatta accgaattaa
     3241 acactcagtt atcacgcaat attgttaatg aggtttttga tgttgctgca ccggcaagag
     3301 gtttagaatt agtgaaaaca gtcgctaatt tgaccgacaa aggtactaaa ctggcgtcat
     3361 ctcaagttta caatcaggct gacggcaagc tctatacttg gaacggtaca gcatactcag
     3421 ctacagtggc tgcggaagat gttaccggca aattatcaaa atctaaaatt gatacgtccc
     3481 ttatttcgca attaacaggt gcagataaca ccgcaaattt ggcaagaaga ttagcggaaa
     3541 cagctcaaag caacattaac caagagatta ctaaccgaca agatgccgtt actgccgaag
     3601 caaataaccg tactaaagcc attcaagccg aaagtgcgaa tttaacgaaa aaaattcaag
     3661 ctgaagcaac cgctcgtggg gctgctgtta cacagttaca aaatgttgat gctcaacaag
     3721 cacagttgat ttctgctgtc acagcaaaag ctgataatgc tctttcaggc ttggaagagg
     3781 aaaaaacagc acgagcaaac ggcgataaag cggagtctca agcacgtgaa gcattaacag
     3841 caagaatggg agcagctgaa agcaatatcg taactattca acgtacggta gcaaataatg
     3901 cccaaagcat tagtgaagtc agtcagaatt taaatgctaa gattgataat attaatatcg
     3961 gcggtcggaa tttattaaga gacagcgagt tcaatgcgta caataaatgg ggtagtccac
     4021 aaattgaatt tgctgaaaat gcaaatcgcc gaactatcaa agtaacctca acaggtacta
     4081 acggtcctgt tggtattgtt tcatctaatc gccattccac atcttatttc caacaaggag
     4141 aaacttatac actctcatta tttgctagag gaagtaaggc actagactat ctctatctaa
     4201 tgcgtcaaga tgggaataac gttagattac cagtgattaa cgttgcctct gaaactgagt
     4261 ttaatcatta caaactcacg ttcaaggctc cttttaccac gcaacaaggt tatgtcttaa
     4321 tcggttttcg gcaaaccagc acagagcagt ttgttgagtt tcacagcgtc aaacttgaaa
     4381 aaggcaacgt agccacagac tggacaccag caccggagga tgtggagagt gcggttagtg
     4441 ctgtttcggc tgatttaacg agttataaac aaactcaagc ggctaccgat tcagcacaag
     4501 cacagcaact taatcagtta tctgtgaact tgaccaaagc agagactaat ttcaacgcca
     4561 aaatcacgga agaaaaaaag ctcgtgttga tgcagataaa gtgaatgcag ataaactcac
     4621 agacataaca agccgtgttg caaatgcgga atcaactatt accaatttcc aatcaaccaa
     4681 agcgaataaa agtgaggtgg caagtattgc ccagcagaac ttacagagca tttggcgcac
     4741 cgatgcgcaa agtgcggtgg atgccttgaa aatcggtggt gcaaatctac tggttgatag
     4801 tgagtatttg acgacagccc gttggggcgg tagctcacga gtggcaagct cgcaatatgg
     4861 tgatcgtcgc ttgactcaag tgtttgtgac ccaagcaggc acagggcatt ttggtgtgac
     4921 tcaaggcaca caaaaagcca ctacgcgtat tcgtcagggt gaaacctata ccttgtcact
     4981 aaatgcgcag gggacggcag gttttacccg cacagggtta aactatgtgt accttatccg
     5041 cgaagatggt ggcaatttta gattgccaac tttgccgctt actgcatcat tgtcccaacg
     5101 ccctaaagtg acatttactg cgccgtggac gagcaatcaa gttcggttac tgattggcgc
     5161 aaatggcata tttgaggcga cagactggtt cgcatttcac agcgttaagc tcgaaatggg
     5221 taatgttgcc acaggctgga caccaaccgc caaggatatt gacgacaaag tatcagcggt
     5281 tcagtccaac ctcaccgctt atcaggcggc acaagctaaa gcagatcaag ccaaagctac
     5341 gcagatttcg gggctcacaa cccgaatggg ggcagcggaa agtaacctca ctcgcaccga
     5401 acgggcagtg acggaactta atcagacaac ggtgacgaca ctgcgtgatc taaccgcgcg
     5461 caccaaaaca acggaaggtt cgctctctcg tttggaaacc gccaaagcaa gtaaaactga
     5521 ggtggcaagt attgctcaaa gctccttaca gtca
//
LOCUS       DANLUQ010000148         5496 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.149, whole genome shotgun sequence.
ACCESSION   DANLUQ010000148 DANLUQ010000000
VERSION     DANLUQ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5496
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.149"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..271
                     /locus_tag="PXB27_002157"
     CDS             <1..271
                     /locus_tag="PXB27_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite reductase flavoprotein subunit alpha"
                     /protein_id="HDL5346904.1"
                     /translation="LTQLFTAFSRDQAEKFYVQDALSQQAELVWHLIQQGAYFYICGS
                     KAMSKAIDAELIKIADQIGGDPYIDDFNNIIAKLVAEGRLMRDVY"
     gene            complement(329..586)
                     /gene="rpsQ"
                     /locus_tag="PXB27_002158"
     CDS             complement(329..586)
                     /gene="rpsQ"
                     /locus_tag="PXB27_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005759643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDL5346905.1"
                     /translation="MTDKIRTVQGKVVSDKMDKSFVVAIERTVKHPLYGKFIRRTTKL
                     HVHDENNEAKLGDVVEIKECRPISKTKSWTLVRVVEKAVVA"
     gene            complement(586..777)
                     /gene="rpmC"
                     /locus_tag="PXB27_002159"
     CDS             complement(586..777)
                     /gene="rpmC"
                     /locus_tag="PXB27_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDL5346906.1"
                     /translation="MKAQELRNKSVEELNGELVNLLGEQFKLRMQAATGQLQQTHQLK
                     QVRRSIAQVKTVLTEKAGE"
     gene            complement(777..1187)
                     /gene="rplP"
                     /locus_tag="PXB27_002160"
     CDS             complement(777..1187)
                     /gene="rplP"
                     /locus_tag="PXB27_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDL5346907.1"
                     /translation="MLQPKRTKFRKVHKGRNRGIAGGTEVSFGSFGLKAIGRGRLTAR
                     QIEAARRAMSRAVKRQGKIWIRVFPDKPITEKPLEVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVSEEVARNAFALAAAKLPFKTTFVTKTVM"
     gene            complement(1201..1908)
                     /gene="rpsC"
                     /locus_tag="PXB27_002161"
     CDS             complement(1201..1908)
                     /gene="rpsC"
                     /locus_tag="PXB27_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDL5346908.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTQDFADNLDGDFKVRKFLN
                     KELANASVSRITIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRNAVAKIAGVPAQIN
                     IAEVKKPELDAKLVADSIASQLERRVMFRRAMKKAVQNAMKLGAKGIKVEVSGRLGGA
                     EIARSEWYREGRVPLHTLRADIDYSTAEAHTTYGVIGVKVWIFKGEILGGMAAVIESE
                     KEPAAQPKKAPRGKGRK"
     gene            complement(1928..2260)
                     /gene="rplV"
                     /locus_tag="PXB27_002162"
     CDS             complement(1928..2260)
                     /gene="rplV"
                     /locus_tag="PXB27_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDL5346909.1"
                     /translation="METIAKHRYARTSAQKARLVADLIRGKKVSVALEILTFTNKKAA
                     TLVKKVLESAIANAEHNDGADVDDLKVAKIFVDEGPSMKRVMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            complement(2271..2546)
                     /gene="rpsS"
                     /locus_tag="PXB27_002163"
     CDS             complement(2271..2546)
                     /gene="rpsS"
                     /locus_tag="PXB27_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDL5346910.1"
                     /translation="MPRSLKKGPFLDLHLLKKVEKAVESGDKKPIKTWSRRSMIIPSM
                     IGLTIAVHNGRQHVPVYVSDEMIGHKLGEFAPTRTYRGHAADKKAKK"
     gene            complement(2577..3398)
                     /gene="rplB"
                     /locus_tag="PXB27_002164"
     CDS             complement(2577..3398)
                     /gene="rplB"
                     /locus_tag="PXB27_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDL5346911.1"
                     /translation="MAIVKCKPTSAGRRHVVKVVNAELHKGKPFAALLDTKSKTGGRN
                     NLGRITTRHIGGGHKQHYRLIDFKRNKLDIPGVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLTAGDQIQAGASAPIKVGNALPMRNIPVGTTVHNVELKPGKGGQIARSAGA
                     YVQIIAREGNYVTLRLRSGEMRKVLAECTATIGEVGNSEHMLRVLGKAGANRWRGIRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRHNKRTDKFIVRRRGK"
     gene            complement(3417..3722)
                     /gene="rplW"
                     /locus_tag="PXB27_002165"
     CDS             complement(3417..3722)
                     /gene="rplW"
                     /locus_tag="PXB27_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005818317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDL5346912.1"
                     /translation="MIQQERLLNVLKAPHISEKATNNAEKGNTIVFKVALDANKVEIA
                     NAVEELFEVKVDSVRTVVVKGKTKRRGAKVGRRSDWKKAYVTLQEGQSLDFVEGAAE"
     gene            complement(3719..4321)
                     /gene="rplD"
                     /locus_tag="PXB27_002166"
     CDS             complement(3719..4321)
                     /gene="rplD"
                     /locus_tag="PXB27_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDL5346913.1"
                     /translation="MELQVVGANALTVSETTFGREFNEALIHQVVVAYAAGARQGSRA
                     QKTRAEVSGSGKKPWRQKGTGRARSGDIKSPIWRSGSVTFAAKPQDHSQKVNKRMYRG
                     AIKSILSELVRQDRLVVVEKFEIDAPKTKVLAQKLKDMALNDALIITANVDENLFLAA
                     RNLYKVDVRDVQGIDPVSLIAFDKVVVTVDAVKQIEEMLA"
     gene            complement(4337..4966)
                     /gene="rplC"
                     /locus_tag="PXB27_002167"
     CDS             complement(4337..4966)
                     /gene="rplC"
                     /locus_tag="PXB27_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDL5346914.1"
                     /translation="MIGLIGRKVGMTRVFTEDGVSIPVTVIEIEANRVTQVKTLENDG
                     YSAIQVTTGSKKASRVTKPEAGHFVKAGVEAGRGLWEFRTTEGEEFTLGQEINVDIFT
                     DVKKVDVTGTSKGKGFQGGVKRWNFRTQDATHGNSLSHRVLGSIGQNQTPGRVFKGKK
                     MAGHLGAERVTVQSLEVVRVDVERKLLLVKGAVPGATNGDVIVKPAVKA"
     gene            complement(4992..5303)
                     /gene="rpsJ"
                     /locus_tag="PXB27_002168"
     CDS             complement(4992..5303)
                     /gene="rpsJ"
                     /locus_tag="PXB27_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDL5346915.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQSTAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHKRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
BASE COUNT         1541 a         1204 c         1013 g         1738 t
ORIGIN      
        1 cttaactcag ctgtttactg ctttttcacg tgatcaggca gagaagtttt atgtgcaaga
       61 tgccctatcg caacaagcag agttagtttg gcatttaatt cagcaagggg cttatttcta
      121 tatttgtggt agcaaagcaa tgagtaaagc aatagatgcg gaactcatca aaattgcaga
      181 tcaaattgga ggcgatcctt atattgatga tttcaataat attattgcaa aattggtcgc
      241 agaaggccga ttaatgcgag atgtatatta gcaaatgaaa aaaactaaaa acccagcaaa
      301 agctgggttt ttatttaatc atagttcatt aagcaacaac tgctttctca actacacgaa
      361 ctaaagtcca agatttagtt tttgaaatag gacgacattc cttaatttca acaacatcac
      421 ctaatttagc ttcattgttt tcatcgtgta catgaagttt agtagtacga cggataaact
      481 taccgtataa cggatgttta actgtacgtt cgatagcaac aacaaatgat ttatccattt
      541 tgtcgctaac cactttacct tgtactgtac gaattttatc agtcattatt cacccgcctt
      601 ttcggttaat acagttttaa cttgtgcaat actacgacgc acttgtttta actggtgggt
      661 ttgttgaagc tgaccggtgg ctgcttgcat acgcaatttg aattgttcac ctaaaaggtt
      721 cactaactca ccattcagct cttcaacact tttattacgt agttcttgag ctttcattac
      781 atcaccgtct tagttacaaa tgtggttttg aacggaagtt tagctgccgc taatgcaaac
      841 gcattgcgtg caacttcttc agaaacacca tccatttcat agagaacttt acccggttgg
      901 attaaggcta cccagtactc aacgttacct ttacctttac ccatacggac ttctaatggt
      961 ttttcagtaa ttggtttgtc tgggaacaca cggatccaga ttttaccttg acgtttaacc
     1021 gcacggctca tcgcacgacg agcagcttca atttgacgag ctgttaaacg accacggcca
     1081 atagctttta aaccaaatga accgaagctc acttctgtgc cacccgcgat accacggtta
     1141 cggcctttgt gtactttacg gaattttgtg cgttttggtt gtaacattca gtgattctcc
     1201 ttatttacga cctttgccac gtggcgcctt tttaggctgt gccgctggtt ctttttcaga
     1261 ttcaatcact gcagccattc caccaagaat ttcacctttg aagatccaca cttttacgcc
     1321 gattacgccg taagtagtgt gagcttcagc agtactataa tcgatgtctg cacgaagcgt
     1381 atgtagaggt acacgacctt cacgatacca ttctgagcga gcaatttctg caccacctaa
     1441 acgaccgcta acttcaactt tgataccttt agcacctaat ttcatcgcgt tttgaactgc
     1501 ttttttcatt gcacgacgga acatcacacg acgttcaagt tgagaagcga tgctatctgc
     1561 gactaatttc gcatctagct ctggtttttt cacttcagcg atgttgattt gagctggaac
     1621 gccagcgatt ttcgcaactg cgttgcgtaa tttttcaaca tcttcacctt ttttaccgat
     1681 aacgataccc gggcgagctg tgtgaatagt tacacgaatg ctttttgcag gacgctcaat
     1741 agtgatgcgt gatacagaag cgttcgctaa ctctttgttt aaaaatttac gtactttgaa
     1801 gtcgccgtct aagttatcag cgaaatcttg tgtattcgcg aaccaagtag agttccaagg
     1861 tttaacaata cctaggcgaa taccattagg atgtactttc tgacccattt gttattctcc
     1921 tacttaatta acgatctgac acaaccacag tgatgtggct tgtacgtttt aaaatacgat
     1981 ctgcacgacc ttttgcacgt ggcattacac gtttcatgct tggaccttca tcaacgaaga
     2041 ttttagcaac tttaagatca tcgacatctg caccgtcatt atgctctgcg tttgcaatag
     2101 ctgactctaa tactttctta acaagcgtcg ctgctttttt gttagtgaaa gttaagattt
     2161 ctaatgctac agaaactttt ttaccgcgaa ttaaatcagc aactaagcga gctttttggg
     2221 cagaagtacg agcgtaacga tgtttagcaa tagtttccat cttttacctc ttatttctta
     2281 gctttcttat ctgcggcgtg accgcggtat gtacgagtcg gtgcaaattc acctaattta
     2341 tgaccgatca tttcgtcgga aacataaaca ggaacgtgct gacgaccatt atggactgcg
     2401 atggtcaaac caatcattga tggaatgatc attgaacgac gggaccaagt tttaattggt
     2461 tttttatccc cgctttccac cgccttctct accttcttca acaagtgtag gtcaaggaaa
     2521 ggacctttct tgagggaacg tggcattggt gtttaccttt tagttaatga attaaattat
     2581 ttgccacgac gacgtacgat gaatttatca gtacgtttgt tgtgacgagt tttcttacct
     2641 ttggtttgaa cgccccaagg tgtaacagga tgtttaccga agttacgacc ttcaccacca
     2701 ccgtgtgggt gatctaccgg gttcattgca gtaccacgaa cagttggacg aatacctctc
     2761 caacggtttg cacctgcttt accaagaacg cgaagcatat gctctgagtt acctacttca
     2821 ccaatggttg cagtacactc agctaatact ttacgcattt cgcctgaacg aagacgtaaa
     2881 gttacatagt taccttcacg ggcaatgatt tgaacgtaag caccagcaga acgtgcaatt
     2941 tgaccacctt taccaggttt taattcaacg ttgtgtacag ttgtacctac tgggatgtta
     3001 cgcattggta atgcgttacc tactttaatc ggagctgaag cacccgcttg aatttgatca
     3061 ccagcagtta aaccttttgg tgctaaaata tagcgacgtt caccatcttt ataaagtact
     3121 aatgcgatat tcgcagaacg atttggatcg tattctaaac gctcaacaac acccggaatg
     3181 tctaatttat tacgtttaaa atcaattaaa cggtagtgtt gtttgtgacc gccaccaatg
     3241 tgacgagttg tgatacgacc taagttgtta cgaccaccag ttttagattt agtatctaaa
     3301 agtgctgcaa aaggtttacc tttgtgaagc tcagcgttta caactttaac aacgtgacga
     3361 cgaccagcgg aggtcggctt acatttaacg atagccattt attttatctc ctctgattac
     3421 tctgccgcac cttcaacgaa gtcaagcgat tgaccttctt gaagagttac ataagctttt
     3481 ttccagtcac tacgacgacc aactttagca ccacggcgtt tagttttacc tttaactact
     3541 acggtacgaa cagaatcaac ttttacttca aaaagttctt caactgcatt tgcaatctca
     3601 actttgtttg catctaatgc aactttgaat acgattgtat tgcctttttc tgcgttgttt
     3661 gtcgcttttt cagagatatg tggtgcttta agcacgttta gcaaacgttc ttgttgaatc
     3721 atgctaacat ctcctcaatt tgttttactg catctacggt tacaaccact ttatcgaaag
     3781 caattaagct aactgggtca ataccttgca catcacgcac atcaacttta tataagttgc
     3841 gtgctgctaa gaataaattc tcatctacgt ttgcagtaat gataagagcg tcatttaacg
     3901 ccatatcttt taatttttgt gctaatactt tagtttttgg tgcatcgatt tcgaatttct
     3961 caacaaccac taaacggtct tgacgaacta attcagaaag aatgcttttg attgcaccac
     4021 ggtacattct tttgttcact ttttggctgt gatcttgtgg tttcgcagca aaagttacac
     4081 tacccgaacg ccaaattggt gatttaatat caccagaacg tgcacggcct gtaccttttt
     4141 gacgccaagg ttttttacct gaaccagaca cttcagcacg agttttttga gcacgagatc
     4201 cttgacgagc acctgctgca taagcaacaa ctacttggtg gattaatgct tcattgaact
     4261 cacgtccgaa ggtagtttca gaaacagtta gtgcgtttgc acctacaact tgtaattcca
     4321 tctctatctc ctagacttat gctttaactg ccggttttac gataacatca ccattagttg
     4381 caccaggaac ggcaccttta actaataata atttacgctc aacatctaca cgaacaactt
     4441 caagtgattg aacggttaca cgctctgcac ctaagtgtcc tgccattttt ttacctttaa
     4501 acacacgacc tggagtttgg ttttgaccaa tagaaccaag tacacgatgt gataaagagt
     4561 taccgtgagt tgcatcttga gtacggaagt tccaacgttt aacaccacct tggaagcctt
     4621 taccttttga tgtaccagta acatcgactt ttttaacatc agtaaagatg tcaacattga
     4681 tttcttgacc taaagtgaat tcttcacctt cagtagtacg aaattcccat aaaccgcgtc
     4741 cagcttcaac acctgctttc acgaaatgac ctgcttctgg tttagttaca cggctagctt
     4801 ttttagagcc agtagtaact tgaattgcag agtagccatc gttttcaagg gttttaactt
     4861 gagtaacacg gttggcttcg atttcgataa cggtaactgg aatagacacg ccgtcttcag
     4921 tgaaaacgcg ggtcatacca actttacgac cgattaaacc aatcattgta ataacctctt
     4981 aaaattccca attaacctag actaatctga acgtcaacgc cagcagctag atctaaacgc
     5041 attaatgcat caacagtttt ttctgttggt tcaacaatat caactaaacg tttgtgagta
     5101 cggatttcat attggtcacg tgcatcttta ttcacgtgtg gagaaatcaa tacagtgaaa
     5161 cgttctttac gagttggtaa aggaattgga ccacgaactt gtgcaccagt acgtttagct
     5221 gtttctacga tctccgcagt agattgatca attaaacgat gatcaaatgc ttttaagcgg
     5281 atacggattc tttggttctg cattagacca gagctccaat taaaaaattt agctaataaa
     5341 aaaaccaaac taccacttaa caaattagtt aagggagctc agttaaacct atatagttcc
     5401 aaaattagga acattgtata cactacaata tctgtagtgc catttgataa atgattacat
     5461 caaatgtctt tagataccta aagctttgct atcata
//
LOCUS       DANLUQ010000149         5474 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.150, whole genome shotgun sequence.
ACCESSION   DANLUQ010000149 DANLUQ010000000
VERSION     DANLUQ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5474
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.150"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(306..917)
                     /locus_tag="PXB27_002169"
     CDS             complement(306..917)
                     /locus_tag="PXB27_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDL5346916.1"
                     /translation="MQLTFNDFQNYYNGKGDLTMFSFYGKKTWENGIYVGIDGSIGRA
                     NNKLSHQSQTIKLARNVYSLGLTVGKTLEVASFNLQPSFGMRYHHLTSANAQLENTKF
                     ETDRVDLLAIQAGLALNKTFIFNELKVKPELGSYFVDASHGTLKTKLNEFSLNQNIGR
                     YFKQEVGITLHYKGVSSSVHAGFTKGNTLQEQKFISLKVGYEW"
     gene            complement(966..1781)
                     /gene="cysE"
                     /locus_tag="PXB27_002170"
     CDS             complement(966..1781)
                     /gene="cysE"
                     /locus_tag="PXB27_002170"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDL5346917.1"
                     /translation="MQQSEINQIWQAIRDEAKELANCEPMLASFFHATILKHHNLGDA
                     LSYILANKLENPIMPAIALKEIIEEAYRAEPQIIASAACDINAVRTRDPAVDKWSTPL
                     LYLKGFHALQSYRVTHYLWNQGRKALAVYLQNEISVAFDVDIHPAAKVGCGIMFDHAT
                     GIVVGETSVIENDVSILQGVTLGGTGKENGDRHPKIREGVMIGAGAKILGNIEIGRYS
                     KIGANSVVLQPVPDHATAAGVPARIIGKSSEQKPAFDMNQYFEDEQGLFGDGI"
     gene            complement(1797..2807)
                     /gene="gpsA"
                     /locus_tag="PXB27_002171"
     CDS             complement(1797..2807)
                     /gene="gpsA"
                     /locus_tag="PXB27_002171"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDL5346918.1"
                     /translation="MSEIYSAPITVLGAGSYGTALAIALSRNGHKTYLWGHNPDKMAV
                     LASERMNQDFLPDIVFPEALEIVADLATAVQKSKDLLIVVPSHVFSEVLAQIKPFLSQ
                     DHRIMWATKGLERDTGRLLQTVAKEILGEIYPLAVLSGPTFAKELAAGLPTAISLAST
                     DPVFADQMQKRSHCSKAFRVYLNNDIIGVQLGGAIKNVIAIGAGISDGMGFGANARTA
                     LITRGLAEISRLGVSLGADPKTFTGMAGLGDLVLTCTDNQSRNRRFGLALGQGKSAEV
                     AIAEIGQVVEGFYNTKEAYLLAQTQGVEMPIVEQIYQMLFCGKQASDVVKTLLGRERK
                     GE"
     gene            complement(2914..3414)
                     /gene="secB"
                     /locus_tag="PXB27_002172"
     CDS             complement(2914..3414)
                     /gene="secB"
                     /locus_tag="PXB27_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HDL5346919.1"
                     /translation="MSEENQVASTEEATQTPFELQIQRIYVKDVSFEAPNLPTIFQQE
                     WKPQLGFELDTETKEIGEDLYEVTLHITVQTTLEDSNDVAFICEVKQAGVFTIKGLEG
                     IQLAHCLASQCPNVLYPYARELISSLVNRGTFPALNLSPVNFDALFMDYLQRQEESAE
                     EPALAN"
     gene            complement(3435..3887)
                     /locus_tag="PXB27_002173"
     CDS             complement(3435..3887)
                     /locus_tag="PXB27_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL5346920.1"
                     /translation="MEQLTFAQQLEQFFANHTLMVFAWVALLIAVIFSFYKSATQKYK
                     IVDNAQATQLINKEEAVVVDLRSDEEFRAGHIIDSIHVLPSDIKAAKINQIDKYKARP
                     VIVVDTNDFTSGGVAADLAKQGFSRVYVLKEGIGGWRAASLPTIKKHK"
     gene            complement(4003..5301)
                     /gene="tig"
                     /locus_tag="PXB27_002174"
     CDS             complement(4003..5301)
                     /gene="tig"
                     /locus_tag="PXB27_002174"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDL5346921.1"
                     /translation="MSFSIETLEGLKRRVTINVPADKIEAAYKEQLKGYAKNARVDGF
                     RKGKVPHSIIEQRFGIAARQDVLSDEMQRAFFEAVIAEKINLAGRPTFTPNNYQPGQD
                     FSFTAEFEVFPEVELKGLENIEVEKPVVEIADADLDKMVDVLRKQQATWSESQEAAKA
                     ESRVTIDFVGSVDGEEFEGGKASDFVLFMGQGRMIPGFEDGIVGRKAGEQFDIDVTFP
                     EEYHAENLKGKAAKFAITLKKVEEMVLPELTEEFVKKFGNAKSVEELRAEIKKNMQRE
                     LKNAVTSRVKTQVINGLIAQNDVEVPAAAVEEEINVLRQQAVQRFGGKPEMAAQLPAE
                     LFEAEAKRRVQVGLLLSSIIGSNELKVDEKRVEEMIADIASAYEQPAEVVAHYAKNRQ
                     LTENIRNVVLEEQAVDAVLAKAKVTEKTMSFDEVMSQQAQ"
BASE COUNT         1561 a         1215 c          962 g         1736 t
ORIGIN      
        1 ctactataga aaactacgaa ctaaatttcc ctatacacga tccgaataat tgtacacaaa
       61 atagcctaag caataaggca ctttgaattt taggagtata tttaggagta caaataaaat
      121 ttgaattaac caaaaccctt atttatcaat gcttgaagtg atacaattca gatgacccca
      181 gcaccaaatt ataatcctat acggttcaac cgatacctaa aaagccttga aaatcaatgt
      241 ttcaaggctt ttttagtaac attatgtttg aaccgattca agtgtagctt caggttacat
      301 aaagcttacc actcatatcc aactttgagt gaaatgaatt tttgttcttg taatgtattt
      361 ccttttgtaa agccagcatg taccgaactt gatacccctt tatagtggag tgtaatccct
      421 acttcttgct taaaataacg gcctatattt tgatttaagg aaaattcatt taacttagtt
      481 ttaagtgttc cgtggcttgc atcaacaaaa tagcttccta attcaggttt tacctttaat
      541 tcattaaata taaaagtttt atttaatgct aaaccagctt gaatggccaa aagatctacc
      601 ctatcagtct caaattttgt gttctctaat tgggcatttg cagatgttaa atgatgatag
      661 cgcataccaa atgacggttg taggttaaaa cttgcaactt ccaacgtttt acctaccgtt
      721 aatcctaaag aatatacatt tctggctagt tttatcgtct ggctttgatg gcttaatttg
      781 ttattcgcac gcccaatgct accatcaatc cctacataaa tgccattttc ccatgttttc
      841 ttaccataaa aagaaaacat agttaaatcc cctttaccat tatagtagtt ctgaaaatca
      901 ttaaaggtta attgcaactt gtacttagca atgtctaaat cataaattaa aggaataact
      961 cttacctaaa tcccatcacc aaacaaacct tgctcatctt caaagtattg attcatatca
     1021 aaagccggtt tttgttcaga ggatttgccg ataatgcgag ccggtacacc tgctgctgtg
     1081 gcgtgatcag gcacaggttg tagcaccact gaatttgccc cgattttaga atagcgtccg
     1141 atttcaatat taccgagaat ttttgcaccg gctccaatca tcacgccttc acggattttc
     1201 gggtggcggt cgccattttc tttccctgta ccgcctagtg ttacaccttg taaaatcgag
     1261 acatcatttt cgatcacaga ggtttcaccc accacaatac cggtggcgtg gtcaaacata
     1321 attccgcaac cgacctttgc cgcaggatga atatccacat caaaggcaac agaaatttca
     1381 ttttgtaaat aaaccgctaa tgctttcctg ccttgattcc aaagataatg cgtcacccga
     1441 tagctttgta aagcgtggaa gccttttaag tacagcagag gtgtactcca tttgtccacc
     1501 gcaggatctc tagttctgac cgcattaata tcacaagctg cactagcaat aatttgtggc
     1561 tctgctcgat aagcttcctc aataatttct tttaaggcga ttgccggcat aatcgggttt
     1621 tctaacttat tcgccaaaat ataacttaac gcatcgccta gattatggtg ttttaaaatg
     1681 gtagcgtgga aaaaacttgc taacataggt tcacaattcg ccagctcttt ggcttcatct
     1741 cgaattgctt gccaaatctg attaatttca ctctgctgca ttttattctc ctttctttat
     1801 tcgccttttc gttcacgccc caacaaggtt ttcaccacat ccgaagcctg tttgccacaa
     1861 aataacattt ggtaaatttg ttctacaatc ggcatttcaa cgccctgtgt ttgagcgagc
     1921 aaataggctt ctttggtatt ataaaatcct tctaccactt gaccgatttc cgctatcgcg
     1981 acttctgccg attttccttg ccctaacgcc aaaccaaatc ggcggtttcg ggattggtta
     2041 tcggtacacg tcagcactaa atctccaagc cccgccattc cggtaaaggt tttcggatca
     2101 gccccgagcg aaacacccaa acggctaatc tccgccagcc ctcgagtaat caaggcggta
     2161 cgagcattcg ccccgaatcc cattccgtct gaaataccgg caccaattgc aatcacattt
     2221 ttaatagccc caccgagttg cacaccgata atatcattgt tgaggtaaac tcggaacgct
     2281 ttggaacagt gactccgttt ttgcatttga tcggcaaaaa caggatcagt tgaggcaagc
     2341 gaaatcgctg ttggcaagcc tgccgccagt tctttggcaa aagtcggacc ggaaagcacc
     2401 gcaagcgggt aaatttcccc caaaatttcc ttagctaccg tttgcaacaa acgcccggta
     2461 tctcgctcca gccctttggt tgcccacatt atacggtgat cttgggacaa aaaaggcttg
     2521 atttgagcca atacttcaga gaatacatga cttggcacca caatcagcag atctttcgat
     2581 ttttgtaccg ctgtagcaag atctgcgaca atttctaagg cttccgggaa tacaatatca
     2641 ggcagaaaat cttggttcat tcgctctgat gccaaaacgg ccattttgtc gggattatgc
     2701 ccccataaat aggttttgtg accatttcga gaaagggcaa tggctaaagc tgttccataa
     2761 gaacctgcac ctaataccgt gattggggca gaatagattt cactcatatt tcctctgttc
     2821 ggcttattta gaaaattgca aaaaatagta gaaaaaataa ccgcttgtaa aaaaataggt
     2881 atgccttgca tacctatttt ctatctaaaa tgcttagttc gctaaagccg gctcttctgc
     2941 actttcttct tggcgttgga gataatccat aaataacgca tcaaagttta ccggtgagag
     3001 atttaatgcc gggaatgtac cacgattcac aaggcttgag attagctcac gagcataagg
     3061 ataaagcaca ttcggacatt gtgaagctaa gcagtgagca agttgaatgc cttctaaacc
     3121 tttaatagta aacacacctg cttgttttac ttcacaaatg aaagcgacat cattgctgtc
     3181 ttctaacgta gtttgcaccg taatgtgtaa ggtaacttcg tataaatctt caccaatttc
     3241 tttggtttca gtatctaact caaagcctaa ttgtggtttc cactcttgtt ggaaaatagt
     3301 cggtaaattt ggtgcttcaa atgaaacatc ttttacataa atgcgttgaa tttgaagctc
     3361 aaaaggggtt tgtgtagctt cttctgttga tgcaacttga ttttcttctg acataaaatt
     3421 tccttaataa tagattattt atgtttttta atagttggta ggcttgctgc acgccagccg
     3481 ccaatccctt cttttaatac ataaactctg ctaaagcctt gtttagcaag atctgccgca
     3541 acaccacctg aagtgaagtc attagtgtct actacaatta ccgggcgagc tttgtattta
     3601 tcaatctgat tgattttagc tgctttaatg tctgacggta acacgtgaat gctatcaata
     3661 atatgaccgg cacggaactc ttcatcagaa cgtaaatcaa ccactactgc ctcttcttta
     3721 ttaatcagct gtgtagcctg tgcgttatcg acaattttat atttttgcgt tgcactttta
     3781 taaaagctaa aaattaccgc aatcaataat gcaacccaag caaacaccat caaggtatga
     3841 ttggcaaaaa attgctctaa ttgttgagca aaagttagtt gttccataat tcaatttcct
     3901 taacatcaaa aaataagcgg tcgttttgat gcaaatttat cactaaattt aaccgcttgt
     3961 aaaagtaata aaaggtgagc aaaagcccac cttataagga aattattgag cttgttgtga
     4021 cattacttcg tcaaatgaca ttgttttttc agtcactttc gctttcgcta gcactgcatc
     4081 aaccgcttgc tcttctaata ccacattgcg gatattttca gttaattgac ggtttttcgc
     4141 atagtgagca accacttcag ccggttgttc gtaagctgaa gcgatatctg cgatcatctc
     4201 ttcaacacgt ttttcatcta cttttaattc gttagaaccg atgattgaag ataataataa
     4261 gcccacttga acacggcgtt tagcttctgc ttcaaataat tcagccggta attgagctgc
     4321 catttcaggc ttaccaccaa aacgttgcac cgcttgttgg cgtaatacgt tgatttcttc
     4381 ttcaaccgca gcagccggca cttcaacatc attttgagca attaagccgt tgattacttg
     4441 agttttcaca cgtgaagtga ctgcattttt aagttcacgt tgcatatttt tcttaatttc
     4501 tgcgcgtaat tcttccactg attttgcatt accgaatttt ttcacaaact cttcggttaa
     4561 ttccggtaat accatctctt ctactttttt caaggtaatt gcaaatttcg ctgctttgcc
     4621 ttttaagttt tcagcgtggt attcttctgg gaaagtcaca tcaatgtcga attgctcacc
     4681 cgctttacga cctacgattc cgtcttcaaa ccccgggatc atacgacctt gccccataaa
     4741 taacacgaag tctgatgctt taccgccttc gaattcttca ccatctactg aaccaacgaa
     4801 atcaatagtt acacgagatt cggcttttgc agcctcttgg ctttctgacc aagtcgcttg
     4861 ttgtttgcgt aatacatcta ccattttgtc taaatcagcg tctgcgattt caacaaccgg
     4921 tttttctact tcgatattct ctaaaccttt taattccact tctgggaata cttcaaattc
     4981 cgcagtaaag ctgaagtctt gacctggttg gtagttgttt ggcgtgaaag tagggcgacc
     5041 tgccaagttg attttttcag caatgacggc ttcaaagaat gcacgttgca tttcatcaga
     5101 taacacatct tgacgggctg caataccaaa acgttgttcg ataattgagt gtggcacttt
     5161 acctttacgg aagccatcta cacgagcatt tttcgcatag ccttttaatt gttctttgta
     5221 tgctgcttcg attttatcag ctggtacgtt aatagtaaca cggcgtttta agccttctaa
     5281 tgtttcaatt gaaaatgaca ttcttaaacc tcggtttagg gatataaaaa agatatacaa
     5341 atagacggtt attgtagcgt tctatcaaga aactgtcgag aagaaactca ttcgcaaata
     5401 aacagttttg taaaaaatcc ctaaaatctg actgcttgtc aatgggcttg cctacaagcg
     5461 gtcttttttc accg
//
LOCUS       DANLUQ010000150         5469 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.151, whole genome shotgun sequence.
ACCESSION   DANLUQ010000150 DANLUQ010000000
VERSION     DANLUQ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5469
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.151"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..2060
                     /gene="glyS"
                     /locus_tag="PXB27_002175"
     CDS             <1..2060
                     /gene="glyS"
                     /locus_tag="PXB27_002175"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDL5346922.1"
                     /translation="QNFLAEIGTEELPPKALKKLATAFAENVENELNQAGLSFEKVEW
                     FAAPRRLAVKVLSLAESQPSKEVEKRGPAVSAAFDAEGKPTKAAEGWAKGCGITVEQA
                     ERIATDKGEWLVHRAVIEGQPTKNLLVDIIAKSLANLPIPKTMRWGDKTEQFVRPVHT
                     VTLLFGAELIEGEILGVKSGRTIRGHRFLGEREFQIENADQYPQLLKDRGSVVADFNE
                     RKALILAKSQEKATALGGVADIEEDLLDEVTSLVEYPNVLTAKFEERFLAVPAEALVY
                     TMKGDQKYFPIYSKDGKLLPHFIFVSNINPEDPSKIIEGNEKVVRPRLTDAEFFFKTD
                     LKQKLEDQLPRLETVLFQQQLGTLRDKTARIEALAGEIAKQIGADETKAKRAGLLSKC
                     DLMTNMVFEFTDTQGVMGMHYARHDGEDEEVAVALNEQYMPRFAGDNLPNSLVACSVA
                     LADKFDTLTGIFGIGQAPKGSADPFALRRAALGSLRIIVEKNLPLDLTDLVAKSAQLF
                     GDKLTNKDVVEDVVDFMLGRFRAWYESEGIAVDVIQAVLARRPTKPADFDARVRAVSH
                     FRTLDSAEALAAANKRVSNILAKADIAIGDIDVSACVEPAEKALAEAVIALKAEVQPL
                     IAQGDYTAVLDKLANLRQPVDAFFDGVMVNAEDQKLRQNRLAILSTLQGLFLQVADIS
                     LLQ"
     gene            complement(2165..3466)
                     /locus_tag="PXB27_002176"
     CDS             complement(2165..3466)
                     /locus_tag="PXB27_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HDL5346923.1"
                     /translation="MNIHCEKLVQSILHAVNHEVVPALGCTEPISLALASAIARQYLG
                     HLPERIEAKVSPNLMKNGMGVAVPGTATVGLPMAAAIGAIGGEPDGGLEVLKNITPEQ
                     VKQAKAMLNTDKVSVSIFETEHILYSEATLFHQANRVCVRIAEHHTNVIYIEKNGQTI
                     LSKPCAVNNDNTTEIFASLSAKDIFNFSMQIELEKIRFIEQAAQLNSALSQEGLRADY
                     GLHIGRTLQKQIGLGLISDDLLNRIVIETTSASDARMGGASLPAMSNSGSGNQGITAT
                     MPIVVVARHLNVTDEQRIRALFLSHLMAIYIHSKLPKLSALCAVTTAAMGSCAGIAWL
                     LTGKFENISMAISSMIGDISGIICDGASNSCAMKVSTSVTSSYKSILMSMDNSQVTGN
                     EGIVEHDIDRSINNLCSIASRSMQYTDRQVIEIMVNKPKLK"
     gene            complement(3480..4757)
                     /locus_tag="PXB27_002177"
     CDS             complement(3480..4757)
                     /locus_tag="PXB27_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346924.1"
                     /translation="MDTQKLSPAEFKQATKFDSTDFGWIIMSIGMAIGAGIVFLPVQV
                     GLMGMWVFLLSSIIGYPAMYLFQRLFINTLAESPECKDYPTVISGYLGKNWGIFLGVL
                     YFIMLVIWMFVYSTAITNDSASYLLTFGITNTLLSDNPFYGLVLICILVALSSRGEKL
                     LFKISTFMVLTKLFVVAALGLAMIGMWQLHNIGAMPPFGKLIKDAIITLPFTLTSILF
                     LQTLSPMVISYRAKEQSREVARYKALRAMNIAFAILFITVFFYAVSFTLAMGREEALK
                     AYEQNVSALAIAAQFFPGSWATYVGVALNIFAVMTAFFGVYLGFREATQGIVMNLLLR
                     KFPAEKINPNYVQKGIMLFSILLAWSAIVLNAPVLSFTSICSPIFGLVGCLIPAYLVY
                     KVPALFKYKGLALNIIIITGILLCISPFLTFLE"
     gene            complement(4786..5454)
                     /locus_tag="PXB27_002178"
     CDS             complement(4786..5454)
                     /locus_tag="PXB27_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HDL5346925.1"
                     /translation="MPDKLEYLHDLDKEIMLSYFSLSEAIAGLLGEFCEVVIHSLNAY
                     EKSVIKIINGHHTNRKIGSPITDKGLQLLKCYQKDQSNTELVYFSRLNNGNLVKSTTH
                     IIIGHEGKPIGLFCINLNLSTPLDKTVSSFMNYDPIPPHYKSENFITHPKELIEETFL
                     KAVTHIEQHTTKHSKLYKKSIIFYLFENGIFELKEAVPIVAEKLSLTSHAIYKHLREF
                     KNNT"
BASE COUNT         1613 a         1106 c         1238 g         1512 t
ORIGIN      
        1 cacaaaactt ccttgccgag atcggcacag aagagctgcc accgaaggca ctcaaaaaat
       61 tagcgaccgc atttgcggag aatgtagaaa acgaattaaa ccaagcaggt ctttcgtttg
      121 agaaagtgga atggtttgct gctcctcgcc gtttggcggt gaaggtgttg agccttgcgg
      181 aaagccagcc gagtaaagag gttgagaaac gagggccggc ggtgtcggca gcgtttgatg
      241 ccgaaggcaa gccgactaag gcagcggaag gctgggcgaa gggctgcggg attaccgtag
      301 agcaagctga gcgtattgcc accgacaaag gcgaatggtt ggtgcatcgt gccgtgattg
      361 aaggtcagcc gactaaaaat ctgttggtcg atattatcgc caaatcgctg gcgaacttgc
      421 cgattccaaa aacgatgcgt tggggcgata aaaccgagca gttcgtccgc cctgtgcata
      481 ccgtgacact tttattcggg gcggagttga tcgaaggtga gatcttaggc gtgaaaagcg
      541 gccgtactat tcgtggtcac cgtttcttag gtgaacgtga gttccaaatt gagaacgccg
      601 atcaatatcc gcaactgttg aaagatcgtg gatctgtggt agcagatttc aatgagcgta
      661 aagccttaat tcttgcaaaa tcacaggaaa aagcaaccgc tttgggtggc gttgccgata
      721 ttgaagaaga tttgctggac gaagtgacct cgttggtgga atacccgaac gtactcaccg
      781 ccaagtttga agagcgtttc cttgctgtgc cggcggaagc cttagtttac accatgaaag
      841 gcgaccaaaa atacttcccg atttattcaa aagacggcaa attgttaccg cactttattt
      901 ttgtgtcgaa catcaatccg gaagacccaa gcaaaatcat cgagggtaac gaaaaagtgg
      961 ttcgcccacg tttgaccgat gcggagttct tcttcaaaac cgacttaaaa cagaagttgg
     1021 aagaccaact gccacgcctt gaaaccgtgt tgttccagca acagcttggc acattgcgtg
     1081 ataaaaccgc tcgcattgaa gcgttggcag gtgaaattgc gaagcaaatc ggggcagatg
     1141 aaaccaaagc gaaacgtgca ggtctcctct caaaatgtga cctgatgacc aatatggtgt
     1201 ttgaatttac tgatacccaa ggcgtgatgg gtatgcacta tgcccgccac gacggtgaag
     1261 atgaagaagt ggcggtggcg ttaaacgagc agtatatgcc acgttttgct ggcgataact
     1321 tgccaaattc attggtggct tgctcggtgg cgttggcgga caaatttgac acgctaacag
     1381 ggattttcgg cattggtcaa gccccgaaag gcagtgccga cccgttcgcc ctacgccgtg
     1441 cggcacttgg ctcgttgcgg attattgtgg agaaaaattt accgcttgat ttaaccgatt
     1501 tagtggcgaa atctgctcaa ttatttggcg ataaactcac caacaaagac gtggttgagg
     1561 acgtggtgga ctttatgctc ggccgcttcc gtgcgtggta tgagagcgaa ggcattgcgg
     1621 tggacgtgat ccaagccgtt ctcgcccgcc gtccgaccaa gcctgccgac ttcgatgccc
     1681 gtgtgcgtgc cgtgtctcac ttccgcaccc ttgacagtgc cgaagcctta gccgcagcga
     1741 ataaacgggt gagtaacatt cttgccaaag ccgatattgc aattggcgac attgatgttt
     1801 cagcttgcgt agagccagca gaaaaagcat tagccgaggc ggttattgcg ttgaaagccg
     1861 aagttcaacc gctgattgca cagggcgact acaccgctgt gctggataaa ttagcgaacc
     1921 tccgccaacc ggttgatgcc ttctttgacg gagtaatggt aaatgcagaa gaccaaaaac
     1981 tgcgtcaaaa ccgcctcgcc attttaagca cgctacaagg cttgttcctg caagtggcgg
     2041 atatttcgct gttgcaataa caataagttt gcaataattg caaaatttta atgaaaatag
     2101 accgcttgca gtaattgtga tctgcaagcg gtcttttttg cttgaaattt taccaaaggc
     2161 gggattattt aagttttggt ttattcacca taatctcgat gacttggcgg tctgtgtatt
     2221 gcatactacg agaggcaatg ctgcataagt tattaattga acgatcaata tcgtgttcga
     2281 caattccttc gttgcctgtt acttggctat tatccataga catcagaatg gatttatagc
     2341 ttgatgttac gctggtggag actttcatcg cacaactgtt tgaagcacca tcacagataa
     2401 taccgctaat atcgccgatc atactactaa ttgccatact gatattctca aatttccctg
     2461 tgagcagcca cgctatgccg gcacagcttc ccatagccgc ggttgttacg gcacagagtg
     2521 cagataattt cggtaacttg ctgtgaatat aaattgccat gaggtgtgat aaaaagagtg
     2581 ctctaatgcg ttgctcatca gtgacattta aatggcgggc aacgacaaca atcggcatag
     2641 ttgctgtaat tccttgatta cctgaacccg aattgctcat agcaggtaaa cttgcaccgc
     2701 ccattctagc atcagaggca gaggtggttt ctataacaat gcgattaagc aaatcatcac
     2761 tgattagacc tagaccaatc tgtttttgca atgtccttcc gatatgtagc ccataatcgg
     2821 cacgtaatcc ttcttgtgag agtgcggaat ttaattgtgc tgcttgctca ataaagcgaa
     2881 ttttttccaa ttcgatttgc atagaaaaat taaatatatc ttttgccgaa agtgaagcaa
     2941 agatctcagt agtattatcg ttattgacgg cacaaggttt gcttaaaata gtttgcccgt
     3001 ttttttcaat gtaaatgaca ttggtatggt gttcagcaat acgaacacat actcgattcg
     3061 cttggtgaaa tagtgttgct tcagaatata gaatatgttc tgtttcgaaa atagagacag
     3121 acactttatc ggtatttaac atagctttag cttgcttaac ctgttctggc gtaatatttt
     3181 ttaatacttc taatccacca tcaggctccc ctccgattgc tccaatagct gccgccatag
     3241 gcaatccgac ggttgcagtt cccggtactg ctactcccat cccatttttc atcaagttag
     3301 gggaaacttt tgcctcaata cgttctggta aatgacctaa atattggcgt gcaatggcgg
     3361 atgcaagggc gagagaaata ggttctgtac agcctaatgc agggacaact tcatggttga
     3421 ctgcatgtaa aatggattgt actaattttt cgcagtgaat attcatagat aaaaccttat
     3481 tactctaaaa atgtgaggaa tggagaaata cataataaaa tgccggtaat gataataata
     3541 ttgagtgcta gccctttata tttgaataat gcaggcactt tatagactaa ataggccgga
     3601 atcaagcacc caactaagcc aaaaatagga ctacagattg aggtaaagct taaaaccggt
     3661 gcatttaata caatcgcact ccaagccagc agaatggaaa ataacataat ccctttctgc
     3721 acataattag gattgatttt ttcagccggg aattttctta ataataggtt catcacaatg
     3781 ccttgagtgg cttctctaaa acctaaatat accccgaaaa aggctgtcat aacggcaaaa
     3841 atatttaatg caacacctac ataggttgcc caactaccag ggaaaaattg ggctgcaatt
     3901 gctaatgcgg aaacattttg ctcgtatgct ttgagcgctt cttctctccc cattgcaagt
     3961 gtaaaagaga cagcgtaaaa aaaaacggta ataaataaga tagcaaatgc aatattcatc
     4021 gctcgtaggg ctttatagcg cgcaacttca cgagactgtt ctttagcacg gtaagaaatt
     4081 accattgggc tgagagtttg caagaagagt atagaggtta gggtgaaagg aagggtaatg
     4141 atggcatctt ttataagttt gccgaaagga ggcattgcac cgatattatg gagttgccac
     4201 attccaatca ttgcaagccc taaggcggca acgacaaata gcttagttaa taccataaat
     4261 gtagatattt taaacagtag cttttcgcct cgagaagata aggcaactaa tatacagatt
     4321 agcactaagc cataaaatgg attatcagag agtagtgtat tggtaatgcc gaaggtaaga
     4381 agataggagg cactgtcgtt tgtaattgcg gtagaataaa caaacatcca aatcactaac
     4441 ataataaaat aaagtacacc taaaaagatt ccccagtttt tgcccaggta gccactaata
     4501 acggtggggt aatctttgca ttccggagat tctgctaaag tattaatgaa cagacgctga
     4561 aaaaggtaca tcgccgggta tccgataata gaagaaagta gaaaaaccca catgcccata
     4621 agtcctactt gtaccggtaa aaagacgatg cctgccccga tagccatacc aatactcata
     4681 atgatccagc cgaagtcggt agaatcaaat tttgttgctt gtttaaattc ggcaggcgag
     4741 agtttttgag tatccataga gtctccttaa atcgtaacag tataattaag tgttattttt
     4801 gaattctctt aaatgtttat aaattgcgtg gctggttagg gagagttttt ctgccacaat
     4861 aggtactgcc tcttttagct caaagatgcc attttcaaac aaatagaaga taatggattt
     4921 cttatagagt tttgaatgtt tggtagtgtg ttgctcaatg tgagtaactg cttttagaaa
     4981 ggtttcttca ataagttctt ttgggtgagt aataaagttt tcacttttat aatgaggcgg
     5041 aataggatcg taattcataa aagagctaac ggttttatct aatggtgtcg ataaatttaa
     5101 attaatacaa aataatccga taggtttgcc ctcatgcccg ataataatat gcgtggtgga
     5161 cttaactaag ttaccgttat ttaaccgtga aaagtaaacc agctcagtat tagattgatc
     5221 tttttggtaa cattttagta actgtaatcc tttatctgta ataggcgaac ctatttttcg
     5281 gttagtatga tgtccgttaa tgattttaat aacggatttt tcataggcat tcaatgaatg
     5341 aataaccacc tcacagaatt ctcctaataa ccctgctatt gcttcactta atgagaaata
     5401 agacagcata atttctttgt ctaaatcatg aagatactct aatttatctg gcataacttt
     5461 tactccaat
//
LOCUS       DANLUQ010000151         5455 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.152, whole genome shotgun sequence.
ACCESSION   DANLUQ010000151 DANLUQ010000000
VERSION     DANLUQ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5455
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.152"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..334)
                     /locus_tag="PXB27_002179"
     CDS             complement(<1..334)
                     /locus_tag="PXB27_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014668079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMP-N-acetylneuraminate-beta-galactosamide-
                     alpha-2, 3-sialyltransferase"
                     /protein_id="HDL5346926.1"
                     /translation="MNLIICCTPLQVLIAEKIIEMHPNERFYGVMLSTVKNAKFDFYQ
                     ARLAQKCEQFFAMQQHSERIQLLKEIIYLKRTFGGKKFNKVFLANINELEIQFLLSAI
                     SFQELNTFD"
     gene            complement(331..1536)
                     /locus_tag="PXB27_002180"
     CDS             complement(331..1536)
                     /locus_tag="PXB27_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HDL5346927.1"
                     /translation="MKAVKDSVIYLIGELASKSIPFLLLPYFSRKLGVAGYGELSYYQ
                     TFAALFVIFIGLSQDGAVARYFYFYGKRSLNLVLSTGYLYTLCATLLILLGCWYFQSE
                     IIAYVAIGAMFQSFLNVQLSVRQCQKQALAYTTIQLMSAIFSAVITVAMMEYFKTDLV
                     EKRILAILMSNGLVFLIAYFLYAKRAKTKKFSLARHKTALLYLFGFGIPLILHHSSIF
                     LKGQLDRVFIYHKFSEEDLGLYAMGAQIAMILAIVVQALNKATLPYFYEALKQKKINV
                     SHIHKWALYALFIVPLPAIVMWFIPEELVVWILGSQFVGTKYYIILFLIATMLVVPYL
                     ILVNYLFFYGKNKLISLCSILSTIFYLVALFALTFSKIEYIPFASIIGAISIIPILYI
                     MTAKVGKTV"
     gene            complement(1546..2730)
                     /gene="neuC"
                     /locus_tag="PXB27_002181"
     CDS             complement(1546..2730)
                     /gene="neuC"
                     /locus_tag="PXB27_002181"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HDL5346928.1"
                     /translation="MKNIAYITGSRAEYGIVKRLLQKLAQDPTVNFSLVVTAMHLDPQ
                     YGNTVTVIEQDGFEIAARIPLTLNSENNQTIIHSMAECLDRFGEHFQQHRYDAVIVLG
                     DRYEMLAVATAAAMHNIPLIHLHGGEQTLGNYDEFIRHCITKMSKLHLTSTEEYRQRV
                     IQMGELPENVVNTGSLGAENSLDLVLPTQAELVERLGIPASPYFLVVFHPETLTDVSV
                     LEQIEALLNAFEQFKDKYQFVFIGSNSDTNSEQIYARFKAYTDENQFKFFTSVKPEEY
                     LALIKYSNGLIGNSSSGLIEAPSCGVGTLNIGNRQQGRVRDESVIDVESNEQAIIDGI
                     EKLISADFQHRLPSMKNPYFQDNAMEKAYNAIKIFLADSHHTQPKGFYDVAFSQAVKK
                     SE"
     gene            complement(2731..3990)
                     /locus_tag="PXB27_002182"
     CDS             complement(2731..3990)
                     /locus_tag="PXB27_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="HDL5346929.1"
                     /translation="MKKIAIITARAGSKGLPNKNVLLANGKPLMAYSIEAAIESGQFE
                     KIIVSTDSQEYIDLFSHYPIEFIKRAEHLASDKASSFVVIEDVLNQYRSVEFDYFMLL
                     QPTSPLRSGEHIKESCEKFEKNFEQFDFLVSVSDAHKPTTLTRMIEEDECLKHFQLDY
                     SNYARQLHHPEYSPNGAIFTAKPEAYLKQKHFYGAKCLAYFMDKDVSIDIDDRQDFEY
                     FYFILQQRNKEKILLETIKRSILMKKENFKEVKEIALIGHSIIEYWDIEHLNGKAVNN
                     LGIAGISTKQYSDLILDEGLISALPKDIILMFGTNDIVYAGWSDEKILADINHLIAQL
                     TAIRGDINLYFLEITPTAFRVDRNNNDIRLLNQYLKDNLAVKWISLDQAFSDKYGKLD
                     LSYSDDGLHLNAKGYEKLTAILEKELN"
     gene            complement(4050..5087)
                     /gene="neuB"
                     /locus_tag="PXB27_002183"
     CDS             complement(4050..5087)
                     /gene="neuB"
                     /locus_tag="PXB27_002183"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HDL5346930.1"
                     /translation="MVNVFIVAEIGCNHNGDPALAKKMVDVAKECGVDAVKFQTFKAD
                     KLISKYAPKAEYQKVTTGTADSQLEMTRKLELPYEEFVKLEAYARSLGLEVFSTPFDF
                     ESIDFLASQQQKVWKIPSGELTNLPYLEKIARLPIEGKTIVISTGMATIEETKASLKV
                     LEDNGVAKKDITILHCNTEYPTPYEDVNLNAFHQLKQEFGDYSLGFSDHSAGYFAGLA
                     AVPYGITFIEKHFTLDKNFEGPDHKASVTPEELKLLCEGIRAVEKSLGSSEKLVTNSE
                     AKNKIVARKSIIAARPIKKGEIFTTENITTKRPGNGISPMFWYDVLGKEAQQDFEEDQ
                     LIQDSRFENQM"
     gene            complement(5087..>5455)
                     /locus_tag="PXB27_002184"
     CDS             complement(5087..>5455)
                     /locus_tag="PXB27_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDL5346931.1"
                     /translation="INVVDKTALVSKNSTLGIGVFVGKMAIVNSGVTVGDNVIINTKS
                     LVEHGCFIGSHCNISTNTTLNGDVIVEDHAFIGSSSVVNGQLRVGESALVGSGAVVIR
                     NVEPRTVVAGVPAKYIKDIK"
BASE COUNT         1725 a         1134 c          904 g         1692 t
ORIGIN      
        1 catcaaaggt attgagttct tgaaatgaaa tggcacttaa cagaaattga atttctaact
       61 cattgatatt tgctaaaaag actttattaa acttcttacc gccaaaagta cgttttaaat
      121 aaataatttc ttttaataat tgaatacgct cgctatgttg ttgcatcgca aaaaattgtt
      181 cgcacttttg ggctaatcta gcttggtaaa aatcaaattt cgcgtttttt accgtagaaa
      241 gcatcacccc atagaatcgc tcattagggt gcatttcaat gattttttca gcaatcaaca
      301 cttgcaaggg cgtgcaacaa ataattaaat tcatacggtt tttcctactt ttgccgtcat
      361 tatataaaga atcggaataa tgctgattgc accaatgatg ctggcaaaag gaatatattc
      421 aattttagaa aaagttaaag caaatagtgc aactaaataa aaaatagtag ataaaatcga
      481 gcaaagcgaa attagcttat ttttgccata aaaaaacaaa taattgacta aaatgaggta
      541 aggcacaacc aacattgtcg caattaaaaa gagaataatg tagtattttg ttcctacaaa
      601 ttggctaccc agaatccata ccactaattc ttccggaata aaccacatca caatagcagg
      661 taatggtaca ataaataagg catacaacgc ccatttgtgg atatggctga cattaatttt
      721 tttctgcttt aaggcttcat aaaaataagg cagtgttgct ttatttaagg cttgaaccac
      781 aatggctaga atcatcgcaa tttgagcccc cattgcatat aaacctaaat cctcttcgct
      841 aaatttatgg taaataaata ctctgtctaa ctgtcctttt aagaaaatac tggaatggtg
      901 caaaatcaac ggaataccaa agccgaataa atacaataaa gcagttttat ggcgggcaag
      961 gctaaatttc ttcgtttttg ctcttttagc atacaaaaag taagcgatta gaaagactaa
     1021 gccattactc atcaaaatcg ctaaaatccg cttttccact aaatcggtct taaaatactc
     1081 catcattgca acggtaataa ctgccgaaaa aatcgccgac attaattgaa tcgttgtata
     1141 agcaagtgcc tgtttttgac actgccttac actaagctgt acattcaaaa acgactggaa
     1201 catcgcccca atcgctacat aagcaataat ttcagactgg aaataccagc aacccaacag
     1261 gataagcaaa gttgcacaaa gagtatataa ataccctgta ctcaagacta aatttagtga
     1321 acgcttacca taaaaataga aatagcgagc aaccgcaccg tcttggctaa gcccgataaa
     1381 aattacaaat aaagcagcaa aggtttggta gtaggaaagc tccccataac ctgccacgcc
     1441 gagtttgcgt gaaaagtagg gtaagagcaa gaaaggaatt gactttgacg ccaattcccc
     1501 aattaaataa atcacactgt ctttaactgc tttcatagtt gcaaattatt ctgatttttt
     1561 gaccgcttgc gagaaagcaa catcataaaa tcctttcggt tgagtatggt ggctgtctgc
     1621 taagaatatt ttgattgcat tgtaagcctt ttccatcgcg ttgtcttgaa aatatggatt
     1681 tttcatactc ggtaaacggt gttggaaatc ggctgaaatc aatttttcga taccatctat
     1741 gatagcctgt tcattactct ctacatcaat tacgctctca tcccgtactc gcccttgctg
     1801 gcgattaccg atatttagcg taccaacacc acaactcggt gcttcaatca agcctgatga
     1861 gctattacca atcaacccat ttgaatattt aatcaacgct aaatactctt ccggtttgac
     1921 tgaggtgaaa aacttaaatt gattttcatc ggtgtaagcc ttaaaacgtg cataaatttg
     1981 ctctgaattg gtgtctgaat ttgagccgat aaagacgaat tggtatttgt ctttaaactg
     2041 ctcaaaagca ttgagtaacg cctcaatttg ctccaacacg gaaacatcgg ttaaggtttc
     2101 cgggtggaag accactaaaa aataaggcga tgccggaatc cctaatctct cgactaattc
     2161 tgcttgggtc ggcaacacta aatccaaact attctccgcc cctagagaac cagtattcac
     2221 cacattttcg ggcagctcgc ccatttgaat cacacgctgt ctatattctt cagttgaagt
     2281 taaatgcaat ttcgacattt tggtaataca atggcgaata aattcatcgt aattacctaa
     2341 tgtttgctca ccaccgtgca gatgaatcag cggaatatta tgcatcgccg cagccgttgc
     2401 caccgccaac atctcgtaac gatcgcctag cacaatcacc gcatcataac gatgttgctg
     2461 gaaatgttcg ccaaatcgat ctaaacactc tgccattgaa tgaataatgg tctgattatt
     2521 ttcactgttt aaggtaagcg gaatacgagc tgcaatctca aagccatctt gctcaatgac
     2581 cgtcaccgta ttgccatatt gcggatccaa atgcatcgca gtaacaacca gagagaaatt
     2641 cacagtcgga tcttgtgcta atttttgtaa taaacgcttt acaatcccgt attcggcacg
     2701 agatccggta atataggcaa tatttttcat ttagttgagc tctttttcca aaatagcggt
     2761 taatttttca taaccttttg catttaaatg taatccgtca tcgctatagc ttaaatccaa
     2821 tttgccatat ttatcgctaa atgcctgatc taagctaatc catttcaccg ctaaattatc
     2881 tttaagatat tgatttaaca agcgaatatc gttgttatta cgatcgactc taaatgcagt
     2941 tggggtaatt tccaagaaat agagattaat atctccacga attgctgtta attgtgcaat
     3001 taaatgatta atatctgcta gaattttttc atcactccag ccagcatata caatatcatt
     3061 agtaccgaac attaaaataa tgtctttagg taaagcagaa attaaacctt catccaaaat
     3121 cagatcactg tattgttttg ttgaaatacc tgcaattcct aaattattaa ctgccttacc
     3181 gtttaaatgt tcaatatccc aatattcaat gatcgaatgc ccgattaagg ctatctcttt
     3241 cacttcttta aagttctctt ttttcattaa aatcgaacgt ttaatggttt caagtaagat
     3301 tttttcttta ttgcgttgtt gcagaataaa gtagaagtac tcgaaatctt ggcgatcatc
     3361 aatatcaata gaaacgtctt tatccataaa ataggctaga catttcgcac catagaagtg
     3421 tttttgttta agataggctt ctggcttagc tgtaaaaata gctccattcg gtgaatattc
     3481 aggatgatgc aattggcgtg catagttaga ataatccaac tgaaaatgct ttaggcactc
     3541 gtcttcctca atcatacgag tcaatgtcgt aggtttatgg gcatcagaaa cagatactaa
     3601 gaaatcaaat tgttcgaaat ttttctcaaa tttttcacac gattccttaa tatgctctcc
     3661 tgatctcaac ggcgaagttg gttgaagtag cataaaatag tcaaactcta ctgatctata
     3721 ttgatttaac acatcttcaa tcacgacaaa agaactcgcc ttatcactgg ctaaatgctc
     3781 ggcacgttta ataaattcaa ttggataatg tgagaacaaa tcaatgtatt cttgggaatc
     3841 agtactaacg ataattttct caaattgccc cgactcaatc gcagcttcaa tagaataggc
     3901 cattaacggc ttaccgttgg ctaataatac atttttattc ggcaagcctt ttgaaccggc
     3961 tcgggctgta ataattgcaa tttttttcat aaatttgacc gcttgttaaa cacaaaatga
     4021 tggagctttc atcccatcac tatattatat tacatctgat tttcaaaacg agaatcttga
     4081 attaattgat cttcttcaaa atcctgttgt gcttctttac ctagcacatc ataccaaaac
     4141 atcgggctaa tgccattacc ggggcgttta gtcgtaatat tttcggttgt aaaaatttcg
     4201 ccttttttaa ttggacgtgc cgcaataatt gattttcttg ccacaatttt attttttgct
     4261 tccgaattag tgactaattt ctcggaagaa cccagcgatt tttccaccgc acgaatgcct
     4321 tcacacagta atttcagctc ttcaggtgta accgaagctt tatgatccgg accttcaaag
     4381 tttttatcta aggtaaaatg tttttcaatg aaggtaatgc cgtaaggtac tgccgccaac
     4441 cctgcaaaat aaccggcaga gtggtcagaa aaacctaaac tatagtcgcc aaactcctgt
     4501 tttagctgat ggaacgcatt taaattcaca tcttcataag gtgtcgggta ctcggtatta
     4561 caatgtaaaa tcgtaatatc ttttttcgct acaccgttat cttccaatac ttttaatgag
     4621 gcttttgttt cttcaatggt tgccataccg gtagaaataa cgatcgtctt gccttcaatc
     4681 ggaagtcgtg caattttttc taaatacggc aaattggtta attcaccaga cggaattttc
     4741 cacacttttt gttgttggct tgccaagaaa tcaatcgact cgaaatcgaa tggcgtagag
     4801 aatacctcta accccaatga acgagcgtaa gcctctaatt tcacaaactc ctcataaggt
     4861 aactctaatt tgcgggtcat ttctaattgg gaatcagcgg tgccggtcgt tactttttga
     4921 tactcggctt taggggcata tttagagatc aacttatccg ctttaaaggt ttggaatttc
     4981 accgcatcga caccacactc tttcgctaca tcaaccattt tctttgctaa cgcaggatcg
     5041 ccgttatggt tacaaccgat ttctgccaca ataaatacat ttaccattat tttatatcct
     5101 taatatattt tgctggaacg cctgctacaa ctgtgcgagg ctccacattt ctaattacca
     5161 ctgcaccgga gccaaccaat gcactttcac ccactcttag ctgtccgttc acaacagagg
     5221 aactaccaat aaaagcatga tcttcgacaa ttacatcgcc atttagtgtc gtattggttg
     5281 aaatattgca atgactgccg ataaaacaac catgctcaac cagcgattta gtgttaatga
     5341 tcacattatc gcctacagtt acgccactgt ttacaattgc catttttcca acaaacactc
     5401 caatgcctag agtggaattt ttggacacca atgcggtttt atctaccaca ttaat
//
LOCUS       DANLUQ010000152         5450 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.153, whole genome shotgun sequence.
ACCESSION   DANLUQ010000152 DANLUQ010000000
VERSION     DANLUQ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5450
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.153"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..207
                     /gene="zapB"
                     /locus_tag="PXB27_002185"
     CDS             <1..207
                     /gene="zapB"
                     /locus_tag="PXB27_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011201404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HDL5346932.1"
                     /translation="VLDQLEEKIKQAVETIQLLQLEVEELKGKNDAAKQENDTLRSEY
                     EQLKVEQQNFQDRLRSLLGQIDNV"
     gene            263..844
                     /locus_tag="PXB27_002186"
     CDS             263..844
                     /locus_tag="PXB27_002186"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014064645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDL5346933.1"
                     /translation="MAKLYFYYSSMNAGKSTTLLQSSYNYQERGMNTLVYTAAIDDRF
                     GVGKVNSRIGISQEANLFTADTNLFAEIKALHSNKALHCILIDEAQFLTKTQVYQLSD
                     VVDKLKIPVLCYGLRTDFQAELFEGSKYLLAWADELEELKTICDCGKKAHFVVRLNEK
                     GEAIKDGEQIQIGGNDRYLSVCRYHYKQKLGKL"
     gene            complement(935..2053)
                     /locus_tag="PXB27_002187"
     CDS             complement(935..2053)
                     /locus_tag="PXB27_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HDL5346934.1"
                     /translation="MTTTYFHLMAKPSSFHCNIQCEYCFYLSKEQTIPPEKSQFMNDE
                     TLQNYIRHYIEANQSQRVDFVWQGGEPTLLGLDFYKQAVKYQQKFANGKQITNAIQTN
                     GIALNRQWASFFKENHFLVGLSIDGLSDIHDRYRISTNGKPTFERVKSAVDLLKEVGV
                     EFNTLTVVNDRNWQRGRETYQALKALGSRYMQFIPVVESHKGELQPFSVPPQGYGQFL
                     VDVFHEWLPDRGQIFVIEFDNLLGQWLGYPSNNCVFRPTCGSSLVVEACGDVYSCDHF
                     VYPDYHIGNLNQQQLAEIVSSAAQRQFGQNKQAKLSQACLNCKFRILCHGGCPKHRIV
                     PQANSENHNYLCPSYQQFFAETSSVMRQMSEQIRFAGK"
     gene            complement(2143..3591)
                     /locus_tag="PXB27_002188"
     CDS             complement(2143..3591)
                     /locus_tag="PXB27_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005822114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDL5346935.1"
                     /translation="MNNIIYILLDQVRKDMLGTYGHQVVKTPNLDRLAKDGIRFNNAF
                     TPASVCGPARTSLFTGLMPSSHGIIKNGEKGGTGEIPQQNPNIAGVEGYNSFVIGKWH
                     VGTKSVPEDYGIAGHNFDGYGYPGSQVYKNLVFNQPPTHSNRYREWLAEKGYDAPEVS
                     RAYFGDNPHLRVQELCGLLSGSREQTIEWFIIDEAKKYIQASLDEKKPFFAWINFWGP
                     HTPCVVPEPYYSMYDPSQVVLDESFFKPLEGKPNHFRTISQMWGMWEASEDRWKEVIS
                     KFWGYITLIDDAIGELFRFLEENNLYENTFLVATADHGDAMGAHKMIEKGEFMFETTY
                     NIPLIIKDPHSTRVNEVDDNLVYLHDLTSTVYDVANQPIPTAFQGESILPITRQSQNN
                     DRKGLLCQLAGHFVYFEQRMWRRKDYKLVFNATDRCELYDVRKDPEEMHNLFYDEAYQ
                     AVKKEMLEEMRQEMKRLNDPLENWVYRIIDEI"
     gene            complement(3738..5435)
                     /locus_tag="PXB27_002189"
     CDS             complement(3738..5435)
                     /locus_tag="PXB27_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HDL5346936.1"
                     /translation="MLLTIITFLVVTGLVGYISWLKTRNDDLSTSKGYFLAGRGLSAT
                     VIGCSMVLTSLSTEQLIGVNANSYAGNFSIIAWTVPAVIPLCFLALYLLPKYIRNGYT
                     TIPEFFENRFDRQTRLIMSGLFLVFYLFIVIPTALYTGAIAFNKIFNLETAFGLSYGA
                     AITYTVIAIGIVGAIYAIFGGLKAVAVSDTWNAVLLVIGALLVPVFALIYLGNGSMAE
                     GVKIITTTHVEKWNAVGSATDAVPWPTIFTGIMVVHFFYWTTNQAIVQRALGARDLKA
                     GQKGILIAALFLLLLPLILNLPGLLSFHILGEGLKPIDVSYPELVNKTFPTVLQGFFI
                     AALFGAILSTFNSFLNSAATIYCKDLLPSISKKQFTDAELISYAKKVSAVMAVITMIF
                     GPLLMFGTDGIFLLTKRFAGFVNIPIVALFVIGLFNKTVSGKAARIALLAHVILYFCI
                     VWVFDVKINFVYVQAGLFVFDVALMLILGIFLKRATPYEDNKINHANVDLTSWEYAPL
                     AVASIVLGLFTLYAFLSPLGLASPDGNPAMVLAVYAVLQAVVCVVFFAKCKGKKAVN"
BASE COUNT         1491 a         1244 c         1244 g         1471 t
ORIGIN      
        1 gttttagacc aattagaaga aaaaattaaa caagctgttg aaactattca attacttcaa
       61 ttagaagttg aagaattaaa aggtaaaaac gatgcagcga aacaagaaaa cgacaccttg
      121 cgcagcgaat atgaacaatt aaaagtggaa caacaaaact tccaagatcg tttacgttca
      181 cttttaggtc aaatcgacaa cgtttaattc tattttcagc acaaacggct aaatttttag
      241 ccgtttttta tttgttacag ctatggcaaa actctatttt tactactcct caatgaatgc
      301 aggcaaatct actactctgc ttcaatcttc ttataattac caagaacgtg gaatgaacac
      361 tttagtttat accgccgcta ttgatgaccg tttcggtgtg gggaaagtca actctcgtat
      421 cggtatttca caagaagcca atttatttac cgctgatacc aatctatttg cggaaattaa
      481 ggcattacac tcaaacaaag cgttacactg tatcttgatt gatgaggctc agtttttaac
      541 taaaacacag gtttatcaat taagtgatgt ggtcgataaa ctcaaaattc cggtgctttg
      601 ctacgggcta cgcaccgatt tccaagctga attatttgaa ggtagcaaat atttgttggc
      661 ttgggcagat gagctggaag aactcaaaac catctgtgat tgtggcaaaa aagcccattt
      721 tgtcgttcga ttaaatgaaa aaggcgaagc cattaaagac ggtgaacaaa tacaaatcgg
      781 cggcaatgat agatatctct ctgtttgccg ttatcattac aaacaaaagt taggtaagct
      841 ataagctaca agaagcagca agcggttaaa ttttcgggaa tttttgcaaa ttttcagcga
      901 aatttaaccg cttgtttgtg ttatttacct ctctttactt tcctgcaaac cgaatttgct
      961 cactcatttg tctcatcaca ctcgaagtct cggcaaaaaa ctgttggtaa gaggggcaaa
     1021 ggtagttgtg gttttcgctg tttgcttggg ggacgatgcg gtgtttgggg cagccgccgt
     1081 ggcaaagtat gcggaatttg cagtttaggc aggcttgcga gagttttgcc tgtttattct
     1141 gcccgaattg gcgttgggcg gctgatgaga cgatctctgc cagctgctgt tggttgaggt
     1201 tgccgatgtg gtaatcggga tagacgaaat ggtcgcaact gtacacatcg ccacaggctt
     1261 ccaccaccag cgagctgcca caggtcgggc ggaatacaca gttgttggag ggatagccaa
     1321 gccattgccc gagcaggtta tcaaactcga tcacaaaaat ctgcccacgg tcaggcagcc
     1381 attcgtgaaa aacatccact aaaaattgcc cgtagccttg gggcggaacg gaaaagggtt
     1441 gtaattcccc cttgtggctt tccaccaccg gaatgaactg catataacgg ctaccgaggg
     1501 ctttgagggc ttgataggtt tctctgcccc gttgccagtt gcggtcgttc accaccgtga
     1561 gggtgttaaa ttccacgcca acctctttca ataaatcgac cgcacttttc acccgttcaa
     1621 aggtcggttt accgttggtg gaaatgcggt agcggtcgtg aatgtcgctc agtccgtcaa
     1681 tggataagcc caccagaaaa tgattctctt tgaaaaagct cgcccactgg cggtttaggg
     1741 caatgccgtt ggtttggatt gcgttggtga tctgcttgcc gtttgcaaat ttttgttgat
     1801 atttgaccgc ttgtttgtag aaatctaagc ccagcagggt cggctcgccc ccctgccaca
     1861 caaaatccac ccgttggctt tggttggctt caatgtaatg acgaatgtag ttttgcagcg
     1921 tttcatcgtt cataaactgc gatttttctg gcggaatggt ctgttccttg ctcagataaa
     1981 aacagtactc gcactgaata ttacagtgaa aactgctggg ttttgccatt aggtggaagt
     2041 aggtggtggt cattgtatct ctaaattgta ggggcggact ttatgttcgc ccattgttta
     2101 cattaatttt cgggcgggta taaaacccgc ctctacaaac aattatatct catcaataat
     2161 ccgatacacc caattttcca gtgggtcgtt gaggcgtttc atttcctgtc gcatttcttc
     2221 caacatctct tttttcacgg cttggtaggc ttcgtcataa aagaggttgt gcatctcttc
     2281 ggggtctttt ctgacatcgt agagttcgca gcggtcggtg gcgttgaaga ccaatttgta
     2341 atccttacgc cgccacattc gctgttcaaa atagacaaaa tgccctgcca gttggcacaa
     2401 caagcctttg cggtcgttgt tttggctttg tctggttatc ggcagaatac tctcgccttg
     2461 gaaagcggtc gggatcggtt gatttgccac atcatacacg gtggaagtga gatcgtgcaa
     2521 ataaacgagg ttgtcatcga cttcgttcac ccttgtagag tgcggatctt taataatcag
     2581 cggaatgttg taggtggttt caaacataaa ctcgcctttt tcgatcatct tatgcgcccc
     2641 catcgcatcg ccgtggtcgg cggtagcaac gaggaaggta ttttcataga gattgttctc
     2701 ttccaagaaa cggaacagct cgccaatggc atcgtcaatc agcgtgatgt agccccagaa
     2761 tttagaaatc acctctttcc aacgatcttc gctcgcctcc cacattcccc acatttgtga
     2821 aatggtgcgg aagtggttcg gtttgccctc aagcggtttg aagaaactct cgtccaatac
     2881 cacttggctt gggtcataca ttgaataata cggctccggc actacacacg gagtgtgcgg
     2941 cccccagaag ttgatccacg caaagaacgg ttttttctca tcaagggagg cttgaatgta
     3001 ttttttcgcc tcgtcaatga taaaccactc tattgtctgc tcgcgactgc cggaaagcaa
     3061 accacagagt tcctgcacac gcaggtgtgg attgtcgcca aaataggctc gactgacttc
     3121 gggtgcgtca tagccctttt ccgccagcca ttcacgataa cggttggagt gtgttggcgg
     3181 ctgattaaac acgaggtttt tatacacttg gctgcccggg tagccatagc cgtcaaagtt
     3241 atgaccggcg atgccataat cttcgggaac ggattttgta ccaacgtgcc atttgccgat
     3301 cacaaaggag ttatagccct ccacgccggc aatattaggg ttttgttgtg gaatttcgcc
     3361 tgtgccgcct ttttcgccgt ttttgataat cccgtggctg gacggcatta agccggtaaa
     3421 cagcgaggtg cgagccggcc cgcagaccga agccggcgta aaggcgttgt taaaccgaat
     3481 gccgtctttc gccaagcgat ccaaattcgg ggtttttacc acctgatgcc cgtaagtgcc
     3541 gagcatatct ttacgcactt gatctaacaa aatatagatg atgttattca taatgacctc
     3601 tttaaggctt gttaataacc gataaattat caagtttcgg agatctgttc ccctctttag
     3661 caaagagggg atttttgatc tcgagccttt tccgttcttg gtatttttat actaatgagg
     3721 agaaggctca cattcattta attcaccgcc tttttgcctt tacattttgc aaaaaacacg
     3781 acacaaacca ccgcttgtag cacagcatac accgccagca ccattgccgg attgccgtct
     3841 ggtgaggcta aaccgagcgg agagaggaaa gcgtatagcg tgaataagcc caacacgata
     3901 gaagccactg ccagtggggc atattcccag ctggttaagt ccacattggc gtggttgatt
     3961 ttgttgtctt cgtatggagt cgcacgtttt aggaaaattc ctaaaatcag cattaacgcc
     4021 acatcgaaca cgaacaagcc agcttgcaca tacacgaagt tgatttttac atcaaacacc
     4081 caaacgatgc agaagtagag aataacgtgt gcgagtaagg caatgcgtgc ggctttgcct
     4141 gagacggttt tgttgaataa gccgatgaca aacagtgcca caatcggaat gttcacaaaa
     4201 ccggcgaaac gtttggtcag caagaaaata ccgtctgtgc cgaacatcag taacggccca
     4261 aagatcatcg taatcaccgc cattaccgca gagacttttt tcgcataact tatcagttcc
     4321 gcatctgtga actgtttttt gctgatagac ggcagtaagt ctttacagta aatggttgcc
     4381 gctgagttta agaacgagtt gaaggtactc aaaatcgccc cgaacagtgc ggcaataaag
     4441 aagccttgta gcacggttgg gaaagtttta ttcactaatt cagggtaaga cacatcaatc
     4501 ggtttcaagc cctcacctaa aatatggaaa ctgagtaagc ccggtaagtt taagataagc
     4561 ggtagcaata ataagaacag tgccgcaatt aagatccctt tttgaccggc ttttaagtct
     4621 ctcgccccca aggcacgttg cacaatggct tggttggtcg tccagtagaa gaaatgcacc
     4681 accataatac cggtgaaaat ggtcggccaa ggcactgcat cggtggcact acccaccgca
     4741 ttccattttt caacgtgagt agtggtgatg attttcaccc cttctgccat actgccatta
     4801 cctaaataga taagtgcaaa caccggcact aataatgccc caatgactaa caagaccgca
     4861 ttccaagtgt ctgacaccgc caccgctttt aagccaccga aaatggcata aatcgcccca
     4921 acaataccaa tcgcaatcac cgtataggta atcgctgccc catagcttaa gccgaaggca
     4981 gtttcaaggt tgaagatttt attgaaggca atcgcaccgg tgtaaagtgc agtcggaatg
     5041 acgatgaaaa gatagaacac caaaaacaag cccgacataa tcaaacgggt ttggcggtcg
     5101 aaacggtttt caaagaactc cggaatggtg gtataaccgt tgcggatata tttcggcaac
     5161 agataaagtg ccaagaagca aagcggaatt accgccggca cagtccacgc aataattgag
     5221 aaattaccgg cataagagtt ggcattcacg ccgataagtt gctcggtaga aagggaggtt
     5281 aagaccatcg aacagccgat aaccgtagca ctcaagccac gccctgccaa gaaataacct
     5341 ttagatgtcg aaagatcgtc gtttctggtt tttagccaag agatataccc gactaatcca
     5401 gttacaacca gaaaggtaat tattgttaat aacatattct tcacctcaat
//
LOCUS       DANLUQ010000153         5341 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.154, whole genome shotgun sequence.
ACCESSION   DANLUQ010000153 DANLUQ010000000
VERSION     DANLUQ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.154"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(281..874)
                     /locus_tag="PXB27_002190"
     CDS             complement(281..874)
                     /locus_tag="PXB27_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346937.1"
                     /translation="MHNNLAIFYCGTNGAGKSTLRSFNQDKVEIVIDSDNIAMQINPL
                     NPRLADIEAGRKAIELFQFAVKNQVAFSMESTLSGKSILKRMENAKAGYFKTRLNYIG
                     VDSYKINIARVKARVKAGGHFIDEETIRKRYHISRENLIEAIFINDETLIYDNSETKP
                     NLILQIADKKVIKISDKIPNWCNQLCDELVELGFKTT"
     gene            complement(864..1034)
                     /locus_tag="PXB27_002191"
     CDS             complement(864..1034)
                     /locus_tag="PXB27_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346938.1"
                     /translation="MTSREELLKKQRELDILFTAWFEEKKKHEVLTYRRENGDLIQHY
                     PDGTEKVIEYAQ"
     gene            complement(1036..1778)
                     /locus_tag="PXB27_002192"
                     /pseudo
     CDS             complement(1036..1778)
                     /locus_tag="PXB27_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
     gene            complement(1775..2218)
                     /locus_tag="PXB27_002193"
     CDS             complement(1775..2218)
                     /locus_tag="PXB27_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL5346939.1"
                     /translation="MLKNLSWRAKRFDELSTQELFEIYHARTAVFVVEQNCPYQEVDN
                     KDLQAVHFFTKNAKNLTAYCRLIPANDGVHIGRVLVVKENRGSGLARELVQKAMDYCG
                     EHFPAQPIHAQAQSYLQGFYESFGFKAVSEVYLEDGIAHLDMVLE"
     gene            complement(2212..2649)
                     /locus_tag="PXB27_002194"
     CDS             complement(2212..2649)
                     /locus_tag="PXB27_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HDL5346940.1"
                     /translation="MKQVQFYLLSQADAAELSAAEMQACELAAQAWRLGKRVLIACET
                     EQQALNIDEALWARDPDSFVPHNLSGEITTYPTPIEISWKGKRNAQRRDLLISLQTQL
                     PEFIGSFTQIIDFVPVEETAKAQARDRYKQYRQLGWQLETIQC"
     gene            2888..3331
                     /gene="lysM"
                     /locus_tag="PXB27_002195"
     CDS             2888..3331
                     /gene="lysM"
                     /locus_tag="PXB27_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HDL5346941.1"
                     /translation="MGLFDFVGDIGRKLFNKEEDASKAVTEHLAEDNPGVENVNVTVE
                     NGVAKISGIASTAAAVEKAVLMAGNVAGITEVNIDEVTLPDDVQLASDDELYVIQKGD
                     TLWEIATKAYGNGAKYKAIVEANKEVIKDENKIFPGQKIRIPKGL"
     gene            complement(3385..4236)
                     /locus_tag="PXB27_002196"
     CDS             complement(3385..4236)
                     /locus_tag="PXB27_002196"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005701564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDL5346942.1"
                     /translation="MKQYLDLCNRIVSEGKWVANERTGKHCLTVINADLTYDVEKGEF
                     PLVTTRRSYWKAAIAELLGYIRGYSNAADFRQLGTKSWDANANENAQWLANPYRKGED
                     DMGLVYGAVGRNFPKPDGGSVDLLRQIVDDLKKGIDNRGEIYTFYHPGAFHMGCLRPC
                     LHSHHFSLLDGTLYLNSTQRSADVPLGLNWNMIQCYTFLALMAQITGHKAGQAFHKIV
                     NAHIYEDQLELMRDVQLKREPLNAPKLIINPDIKSLEDLETWVSLDDFKVEGYEYHPA
                     IQYPFSV"
     gene            4329..4880
                     /locus_tag="PXB27_002197"
     CDS             4329..4880
                     /locus_tag="PXB27_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346943.1"
                     /translation="MKGIMKSILVFSTAVFLAACSSNKSGWTRLNDNDVDQKSYAIAY
                     GATAQTYADRVNESYDINSFMNGVNDWYTKKVTLPIEQIRAITINRMLDHNVYAYYSG
                     VIYAADLQANFNHLDPNCWGLVQPASMTQGIHDAMLDLQKNKVRGDEYIKNGVEQILH
                     LCVKTIAEDENESQSKGKTKKHK"
     gene            5089..5183
                     /locus_tag="PXB27_002198"
     tRNA            5089..5183
                     /locus_tag="PXB27_002198"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5124..5126,aa:Ser,seq:gct)
     gene            5197..5273
                     /locus_tag="PXB27_002199"
     tRNA            5197..5273
                     /locus_tag="PXB27_002199"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5231..5233,aa:Arg,seq:acg)
BASE COUNT         1566 a         1023 c         1051 g         1701 t
ORIGIN      
        1 aataactttt ttagactttt tcctattctg tttcatcttt gtgtttttgt tccaatatcc
       61 ccgatggtga tcgtcttcga cgagagctta tttatttaag aacctatcag agataggcat
      121 aagcggtctg tttcgagaag ttttttgcaa agtttttctt taatccgaca gcttgtcacc
      181 cacacacaag ccgtgtgggg ttacgcatag ctgagccact ttgtggctct aagttacagc
      241 tccaaaggag ctgggattgt cgagcctagc acggctcgtg ctaggtggtt ttaaacccta
      301 attccactaa ttcatcacaa agctgattac accaattcgg tattttgtct gaaattttta
      361 tcactttctt atcagcaatc tgcaaaatca aattcggctt ggtttctgaa ttatcataaa
      421 tcagtgtttc atcattgata aaaatcgctt caattaaatt ttcacggcta atgtgatagc
      481 gttttctaat ggtttcttca tcaataaaat gtccgccggc tttcactctt gcttttaccc
      541 gtgcgatgtt aattttatag ctatcgacac caatataatt taatcgagtt ttgaaatatc
      601 ctgctttggc attttccatt cgttttagaa tagatttacc cgagagggtt gattccattg
      661 aaaatgcaac ctgatttttt actgcaaact gaaatagttc gattgctttt ctgcccgcct
      721 caatatcggc caatcttgga ttgagtgggt taatctgcat cgcaatatta tcggaatcaa
      781 tcacaatctc gactttatct tgattgaaag aacgtaaagt agatttgccg gctccgtttg
      841 tgccacagta aaaaattgct aggttattgt gcatattcaa tcactttttc cgtaccgtcc
      901 ggataatgct gaattaaatc cccattttct cggcgatacg tcagcacttc gtgctttttc
      961 ttttcttcaa accatgctgt aaagagaata tccagttcac gttgtttttt cagtaattct
     1021 tcacgacttg tcattttatg tcctctgttt cagaatcgcc aaataccttt ccaactcagc
     1081 taattcatac tgactaatca attcaaccaa actactaata tcttgttgat ttttatggaa
     1141 atcatcaaaa cattggtaat attgggctcg attggtaaat ttaatatcaa tcggtaaata
     1201 gccctgtttt aataattcaa aattaatcag taatcgccct gtgagcccgt ttccatcaat
     1261 aaaagggtga atgctttcaa aggctaaatg cagccaactg attgcctcta tgggatgaag
     1321 tgcggtcagt ttttgttgat atttttcaat caataattgc atttcttcgg caattaaata
     1381 aggggggggg cattttctgc ccctaaaatt cgtactggaa ttttgcgata aacccctcgg
     1441 ctttccgcat tgcccattaa taccagagaa tgaatatctt taatcgctcg ttcgcttaat
     1501 ggttctttat tggcgactaa ttcataaata tagttgaatg catccttaaa accgataata
     1561 tctaaatgct cccgaatcgg tttttcggca atggtcattc cctcttttaa aatgagcacc
     1621 gtttcacgca aagtaatggt attgccttca attgcattgg aattatagct gctttcaata
     1681 ataaactctt cccgcagtcg ttgaacttcg ctttcagata atggacggcg ttgtgaaagt
     1741 gctgttttta attgttcgat tttagctaat ttattcattc taacaccata tccaaatgcg
     1801 caatcccatc ttccaaatac acttctgaaa ccgctttaaa cccaaaactt tcataaaacc
     1861 cttgcaggta actttgggct tgggcgtgaa tgggttgagc aggaaaatgt tcgccacaat
     1921 aatccatcgc tttttgtacc aattcccttg ctaagcctga gccacgattt tctttcacca
     1981 ccaacactct acctatatgc acaccatcat tggctggaat taaacggcag taagcggtca
     2041 gattttttgc attttttgta aagaaatgca ccgcttgcag gtctttatta tcgacttctt
     2101 ggtaagggca gttttgctca accacaaaca ccgctgtacg tgcgtggtaa atctcaaaca
     2161 actcttgggt ggagagttca tcaaagcgtt ttgcgcgcca agagaggttt tttagcattg
     2221 tatcgtttcc agttgccaac caagttggcg gtattgttta tagcggtcac gagcctgtgc
     2281 tttggcggtt tcttccaccg gcacgaaatc aataatctgg gtaaaactgc cgataaattc
     2341 ggggagttgg gtttggagtg aaatcaacaa atcccgacgt tgagcattgc gtttaccttt
     2401 ccacgaaatt tcgataggcg ttgggtaagt ggtgatttct cccgataaat tgtgcggcac
     2461 gaacgaatcc ggatctcttg cccaaagtgc ttcgtcaata tttaacgctt gttgctcggt
     2521 ttcgcatgca atcagtaccc gtttacctaa tcgccacgct tgagccgcaa gttcgcaggc
     2581 ttgcatttca gcagcggaaa gctcggctgc atcggcttgg cttaatagat aaaattgtac
     2641 ttgtttcaca gaatgttccc agaaattcaa tcagatattt aaacttaata ttctagctga
     2701 aaatgagtaa aatataaaat ctaagcgttt gtttttgcaa aaaattgaga aaaaatgacc
     2761 acttgtgttg cgttgccttg aggaactaat tagcttttag acgttctaag agaacaatgc
     2821 actatttcaa tagttggaaa ttgtgtagaa tacccacttc atttagctat tttataagga
     2881 acttactatg ggcttatttg attttgttgg cgacattggt cgtaaattat ttaataaaga
     2941 agaagatgct tcaaaagcgg ttacagagca tttggcggaa gacaatccgg gcgttgaaaa
     3001 tgtaaacgta acggttgaaa acggggtagc gaaaatttca ggcattgctt caaccgcagc
     3061 agctgttgag aaagcggtat tgatggcggg taatgtggct ggtatcactg aggtaaatat
     3121 tgatgaagtg accttgcctg atgacgtgca acttgcaagt gatgatgagc tttatgttat
     3181 ccaaaaaggg gatacgttgt gggaaatcgc cacaaaagct tatggtaacg gtgcaaaata
     3241 taaagcgatt gtagaagcaa ataaagaagt tattaaagac gaaaataaaa tcttcccagg
     3301 gcaaaaaatt cgtatcccga aaggtttata atcaaattga aagagctaag ctggtcgaaa
     3361 gtttagctct ttttgtaggt ggtgttagac tgaaaatgga tattgaatgg cagggtggta
     3421 ttcataacct tcaactttga aatcatctaa gctcacccaa gtttctaaat cttctaagga
     3481 ttttatatca ggattaatga ttaatttagg tgcattgagt ggttctcgct taagttgaac
     3541 atcacgcatt aattctagtt gatcttcata aatatgcgca ttgacaattt tatggaacgc
     3601 ttgtcctgcc ttatgccctg taatttgagc cattaaagct aaaaaggtat agcattgaat
     3661 catattccaa tttaacccaa gcggtacatc tgccgaacgc tgagtactat tcaggtaaag
     3721 cgtgccatca agtagagaaa aatgatggct gtgtaaacaa ggacgtaagc agcccatatg
     3781 gaaagcgccg gggtggtaga aggtataaat ttcaccacgg ttatcaattc cttttttcaa
     3841 atcgtccaca atttgtcgca ataaatcgac tgagccaccg tctggtttcg ggaaattacg
     3901 ccctactgcg ccgtaaacca agcccatatc atcttcccct tttcgatagg gattagcaag
     3961 ccattgtgca ttttcatttg cattagcgtc ccacgatttt gtgcctaatt gacggaaatc
     4021 agcagcatta ctatagcctc gaatatagcc taaaagttcg gcaatcgcag ctttccaata
     4081 actacggcga gtggtaacta aaggaaattc tcctttttct acgtcataag ttaagtcagc
     4141 attaatgact gtgaggcagt gtttgcctgt acgttcattt gcaacccatt tgccttcgct
     4201 aacgatgcgg ttacataagt ctaaatactg tttcattatt tacctcataa aaaattttag
     4261 gtaattttgc catttattcg gtaaaatagc taaaacttta tgacacaaca caataaggag
     4321 ttatggatat gaaaggtata atgaaatcca ttctggtttt ttccaccgct gtttttttag
     4381 cagcttgttc atcgaataaa agcggttgga cacgtttaaa tgataatgat gttgatcaga
     4441 aatcttatgc tattgcttat ggtgcaacgg ctcagactta tgcggataga gtaaatgaaa
     4501 gctatgatat caattccttt atgaatgggg taaatgattg gtacactaaa aaggttacat
     4561 tgccaattga gcaaattaga gcaataacga ttaatcgaat gttagaccat aatgtgtatg
     4621 cttattatag tggtgtgatt tatgccgcag atttgcaggc aaatttcaat catttagatc
     4681 ctaattgttg ggggcttgtt caaccagcga gtatgaccca gggcattcac gatgcgatgt
     4741 tagatttaca gaagaacaaa gttcgtggag atgagtatat caaaaatggg gttgagcaaa
     4801 tattgcacct ttgtgtaaaa acgattgctg aagatgaaaa tgaatcgcaa tctaaaggta
     4861 aaaccaaaaa gcataaataa gttaagctat gacaagcggt aaaattttta aaaaatttta
     4921 ccgctttttt ataaccgtat gaaatgttta atatttattc tatgaaactt aaaaaaacag
     4981 cgttttgttt attcaatagg caaacactcg ggatttttca attttcagga aaaagaattt
     5041 gacatctttt taaattctag taatatggcg cccacagaca cagtgagtgg tgagatggcc
     5101 gagcaggctg aaggcgctcc cctgctaagg gagtataggg ttaaaaagct ctatcgaggg
     5161 ttcgaatccc tctctcaccg ccatttactt tatcacgcac ccgtagctca gctggataga
     5221 gtactcggct acgaaccgag cggtcagagg ttcgaatcct ctcgggtgcg ccattttaat
     5281 ttttactcta ttcaaattaa aatggttgct gataaaatta aataaactta caattcaaat
     5341 a
//
LOCUS       DANLUQ010000154         5309 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.155, whole genome shotgun sequence.
ACCESSION   DANLUQ010000154 DANLUQ010000000
VERSION     DANLUQ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5309
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.155"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            43..1677
                     /locus_tag="PXB27_002200"
     CDS             43..1677
                     /locus_tag="PXB27_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005700110.1"
                     /note="is involved in the transport of the murein peptide
                     L-alanyl-gamma-D-glutamyl-meso-diaminopimelate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HDL5346944.1"
                     /translation="MQSKFSLSLLTTSLVLGLSANVFAAKVPEGTTLAEKQEITINNG
                     FEPSSFDPHKIEGVPESQVAYQLFEGLVTKDSDGNIQPGVAESWENTPDYKTWTFKLR
                     QDAKWSNGDAVTAHDFVFAWQRLVNPLTASPYSSYLTYLNVENAQDIIDGKKKPEELA
                     VKAIDDHTFQVQLSQPVPYAVELATHSSLLPVHKATVEKHGDAWVQKGNLVGNGAYTL
                     ADHVINEKIVFERNKNYWNDKETVINKATFLAIANAATDVSRYRAGDLDMTNYALPPE
                     QFATLKKEIPNEVFTARTLSTYYYEINHKRAPFDNVKVREALNLALDRTIITDKVLGQ
                     GQTPTYVFTPPYIHEGEKIQQPAYSKDSMADRKAKALKLLEEAGYSKANPLKFTILYN
                     TNDNHKKIAIAVSSLWKQGTDGIVDVKLENQEWKTFLDTRRNANYDVSRAGWSADYNQ
                     ATTFVNYFLSNSSNNTAFYKSQAYDDAVAESYKATDAAGRAAAYAKAEEQLAKDYAII
                     PIYNYVNPRLVKPYVKGYEGKDPQDDILLRNLYIVK"
     gene            1731..2003
                     /locus_tag="PXB27_002201"
     CDS             1731..2003
                     /locus_tag="PXB27_002201"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDL5346945.1"
                     /translation="MVAKLFSVYGRVQGVAFRFFTLQEANRLGIKGYVRNRDDGSVEV
                     VAEADEQTIAQLENWLQQGSPAAKVERVIVSDYLGSERFKRFEVRY"
     gene            complement(2063..4675)
                     /gene="pepN"
                     /locus_tag="PXB27_002202"
     CDS             complement(2063..4675)
                     /gene="pepN"
                     /locus_tag="PXB27_002202"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HDL5346946.1"
                     /translation="MQPKAKLRKDYRAPDFTITDIYLDFQLDPDRTFVTSKLTVVRKN
                     PEATTLRLDGHSFDFLSLKYNGEPFSAFQKDDESLTLNLADVPAERFELEIETALNPA
                     QNTSLQGLYKSGDGFCTQCEAEGFRQITYMLDRPDVLAKYTTKITASKSKYPYLLSNG
                     NHIAQGDLHDGRHWVEWQDPFFKPSYLFALVAGDFDLLQDKFITKSGREVTLEIYVDR
                     GNLDRAPWAMESLKRSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKF
                     VLAKPETATDTDYLDIEAVIAHEYFHNWTGNRITCRDWFQLSLKEGLTVFRDQEFTSD
                     LWSRSAKRIEDVRLLRTVQFAEDASPMAHPIRPEKVIEMNNFYTVTVYEKGAEIIRMI
                     HTLLGEEKFQQGMQLYVARHDGSAATCEDFVKAMEDASGVNLEQFRRWYSQSGTPELT
                     ISDEYDETRKAYRLHISQMTPATHDQMDKLNLHIPLKVALYGEQDGKRIPLQYELLTV
                     SDVLDVTAEHQTFEFHNVTQRPVPALLCDFSAPVRLDYNYTTEQLLTLLKHAENDFVR
                     WDAAQMLYTNELRENLSRYQQHQPLSFSEGLVNALTFVLDNSASLPELTALTLTLPKE
                     TEFAESFKTIDPQGIAVVREFMQHSIAHALQDKLLAVYHQNQCGEYQVVAEDIAKRAL
                     RNACLSYLAFTDLGNALVHKHYNNADNMTDTLAALQAATKAQLSCRDALLADFEQKWK
                     HDGLVMDKWFMLQATRPDDDVLSIVQNLMEHPSFNFNNPNRLRSLVGAFCGQNPKAFH
                     AIDGSGYRFLVDTLIKLNESNPQVAARLIEPLIKLSRYDNQRQTLMRRGLERLRELDN
                     LARDLFEKIEKALD"
     gene            complement(4825..>5309)
                     /gene="hutZ"
                     /locus_tag="PXB27_002203"
     CDS             complement(4825..>5309)
                     /gene="hutZ"
                     /locus_tag="PXB27_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heme utilization protein HutZ"
                     /protein_id="HDL5346947.1"
                     /translation="LGPEIEELKQQIKTIMLATIDKDGVPNVSYAPFVINNGEYQVLI
                     TTIARHARNLQEVPKVSLMLIEDESKSRQIFARRRLTFDATVRMIERTDEEWNNSIAA
                     LKARHGDLVDELSNMQDFKLFSFKPTAGLFVKGFGKAFDVGVDDLINVVHLDQGHQTA
                     "
BASE COUNT         1553 a         1069 c         1151 g         1536 t
ORIGIN      
        1 atcggctggt tgcaagccaa tattcatcaa tggagatcaa atatgcagtc aaaattctcg
       61 ctttccttat taacaacctc attagtgtta ggcttatcgg caaatgtgtt tgcagccaaa
      121 gtgcctgaag gaacaacgct tgcagaaaaa caagaaatta cgattaataa tggttttgag
      181 ccatcaagtt ttgatccaca taaaattgag ggcgtgccgg aatcgcaagt tgcttatcag
      241 ttatttgaag gattggtaac gaaagattct gacggcaata ttcagccggg tgtggcagaa
      301 agttgggaaa atacaccgga ttacaaaacg tggacgttca aattacgcca agacgctaaa
      361 tggtcaaacg gtgatgccgt aactgctcac gatttcgtat ttgcttggca gcgtttggtt
      421 aatccactta cggcttcacc ttattcaagc tatttaactt atctcaatgt agaaaatgct
      481 caagatatta ttgacggcaa gaaaaaaccg gaggaactag cagttaaagc gattgatgat
      541 catactttcc aggtgcagtt aagccaacct gtcccgtatg cagttgagct ggcaacacac
      601 tcttctttat tacctgtaca caaagcaacc gttgagaaac acggcgatgc gtgggtgcaa
      661 aaaggcaatc tggttggaaa cggggcttat accttagccg atcacgttat taatgaaaaa
      721 atcgtgtttg agcgtaataa aaattattgg aatgataaag aaaccgtgat taataaagcg
      781 actttcttag ccattgctaa tgccgcaact gatgtttcac gttaccgtgc cggtgatttg
      841 gatatgacca actatgctct tccaccggag cagtttgcaa ccttgaaaaa agagattcca
      901 aacgaagtgt ttacggcaag aaccctttct acttattact atgaaattaa ccataaacga
      961 gcaccgtttg ataatgtgaa agtgcgtgag gcgttaaact tagctttaga tcgcaccatt
     1021 attaccgata aagtgctagg acaaggtcaa acaccaactt atgtatttac accgccttat
     1081 attcacgagg gtgaaaaaat ccagcaacct gcttattcca aagattcaat ggcagataga
     1141 aaagccaaag cgttgaaatt attggaagaa gccggttaca gtaaagcgaa cccgttaaaa
     1201 tttactattc tttataatac caacgacaac cacaagaaaa ttgcgattgc agtttcatca
     1261 ttatggaaac aaggcacaga cggcattgtt gatgtgaaat tagaaaacca agagtggaaa
     1321 acattcttag atacccgccg caatgcaaat tatgatgttt ctcgtgcagg ttggtcggca
     1381 gactataacc aagcaaccac ctttgtgaac tatttcttat cgaactccag caacaatact
     1441 gcgttttata agagccaagc ttatgatgat gcggtagcag aatcttacaa agcgacagac
     1501 gcagctggtc gtgcagctgc ttatgcaaaa gcagaggagc agttagccaa agattatgcg
     1561 attattccga tttataacta tgtaaaccca cgtttggtga aaccttacgt gaaaggctat
     1621 gagggtaaag atccgcaaga tgatatttta ctgcgtaacc tctacatcgt gaaataagac
     1681 attatgggct gtttattgaa aaataagcag cctattttta tgggggaaga atggtcgcaa
     1741 aattattctc ggtttacgga cgggttcaag gtgtcgcttt tcggtttttt accttgcagg
     1801 aagcgaatcg attaggcata aaaggctatg tcagaaatcg tgatgacggc tcggtagaag
     1861 tggtagctga agcggatgaa cagacaatcg ctcaactgga aaattggtta caacaaggct
     1921 cacctgctgc aaaggtggaa cgggtaattg tgagcgatta tcttggctca gaaaggttta
     1981 aaaggtttga ggtaaggtat taactaacaa gcggtgtcat ttgtaaaaaa atttgcaaac
     2041 gacaccgctt gtattgcttg gtttaatcta acgctttttc aattttctca aacagatctc
     2101 gtgctaaatt atccagctca cgcaggcgtt ctagaccacg acgcatcaag gtttggcgtt
     2161 ggttatcgta gcgtgagagt ttaattaacg gctcaataag tcgagcagca acttgtgggt
     2221 tactttcgtt aagtttaatc agcgtatcta ccaagaaacg atagcctgaa ccgtcaatgg
     2281 cgtggaaggc tttcgggttt tgaccgcaga atgcacctac taatgaacgt aaacggttcg
     2341 ggttattaaa gttaaaactt gggtgttcca ttaaattttg cacgatggat aacacatcgt
     2401 cgtccggtcg ggtggcttgt agcataaacc atttatccat cactaaacca tcatgtttcc
     2461 atttttgctc aaaatcggct aataatgcat cacggcaact tagctgggct ttggttgcgg
     2521 cttgtaatgc agcaagcgta tcagtcatat tatcggcatt attgtaatgc ttatgcacca
     2581 acgcgttgcc taaatcagta aaggctaaat agcttaagca agcattacgc aaggctcgtt
     2641 tagcaatatc ttcagccacg acttgatatt ccccgcattg gttttggtga tataccgcta
     2701 ataacttatc ttgcaaggcg tgagcaatgg aatgctgcat aaattcacgc acaaccgcaa
     2761 taccttgtgg gtcaatggtt ttgaacgatt ctgcaaactc ggtttctttt ggcaatgtga
     2821 gggttaatgc agttagttcc ggcaggcttg ctgagttatc caacacgaac gttagggcat
     2881 tcaccaaccc ttctgagaag ctgagtggtt gatgttgctg ataacggctt aaattttcac
     2941 gcagttcatt ggtatagagc atttgagctg catcccagcg tacaaaatca ttttcagcgt
     3001 gtttgagtaa ggttaaaagc tgctcggtgg tatagttgta atctaaccgt accggtgcag
     3061 agaaatcaca cagtaatgca ggcaccgggc gttgtgttac attatgaaac tcaaaggttt
     3121 ggtgttctgc ggttacatct aacacatcgc ttacggtcaa taattcatat tgtaacggaa
     3181 tgcgtttacc gtcctgttca ccatataacg ctactttaag cggaatatgc agattgagtt
     3241 tatccatctg atcgtgggtt gccggtgtca tttgtgaaat atgcaagcgg tatgccttgc
     3301 gggtttcatc atattcatcg gaaatggtga gctcaggcgt gccggattgg ctataccaac
     3361 ggcggaattg ttcaagatta acgcctgaag catcttccat tgctttaacg aaatcttcac
     3421 aagttgccgc agaaccatcg tgtcttgcca cataaagttg cataccttgc tggaattttt
     3481 cttcgcctaa cagggtgtga atcatacgaa taatttccgc ccctttttca tacacagtca
     3541 cggtgtagaa attattcatt tcaatcactt tttccgggcg aatcgggtga gccatcgggc
     3601 tggcatcttc ggcaaattgt acggtacgca gtaaacgcac atcttcaatc cgttttgccg
     3661 aacgagacca taaatcggag gtaaattcct gatcacggaa gacggttaag ccctctttta
     3721 agcttagttg gaaccaatca cggcaggtaa tgcggttgcc cgtccaatta tggaagtatt
     3781 cgtgggcaat caccgcttca atatctaaat aatccgtatc ggttgcggtt tccggttttg
     3841 ccaatacaaa tttggaattg aaaatattta accctttgtt ttccattgct cccatattga
     3901 agaaatcaac ggcaacaatc atataaatat ctaaatcgta ttccaaaccg aaacgctctt
     3961 catcccattt cattgaacgt tttaagctct ccatcgccca aggtgcacgg tctaaatttc
     4021 cacgatcgac ataaatttca agcgtgactt cacgtccgct tttggtgatg aatttatctt
     4081 ggagtaagtc aaaatccccc gcaaccaagg caaataaata gcttggttta aagaacggat
     4141 cttgccattc cacccagtgt ctgccatcgt gcaagtcgcc ttgggcaatg tggttgccgt
     4201 tagaaagcag atacgggtat ttgctcttac tagcggtgat ttttgtcgta tattttgcaa
     4261 gtacatcagg gcggtcgagc atataggtaa tctggcggaa accttcagcc tcacactggg
     4321 tgcaaaaccc gtcgcccgat ttatacaaac cttgtaacga ggtgttttgt gccggattta
     4381 atgcggtttc aatttcaagc tcgaaccgct cggcaggcac atcggctaaa tttaaggtta
     4441 aagattcatc atctttttga aaggcggaaa acggctcgcc gttatattta agcgaaagga
     4501 aatcgaaact atgaccgtct aaacgaagtg ttgtcgcctc aggattttta cgcacgacag
     4561 ttagttttga ggtgacaaaa gtgcgatctg ggtcaagttg gaagtcaaga taaatatcag
     4621 taatagtgaa atcgggagct cgataatctt ttctgagttt cgctttaggt tgcataggat
     4681 ttcctttttt tagttgcgtt tagctcatag aatagtgtcg ttattgtact aaaatagcgg
     4741 taaattttcc aaaaaatttg accgcttgtc gcaataaaaa cggctataaa taggattatt
     4801 tatagccgtg agtttatttc gcaattaggc agtttggtgg ccttggtcta aatgaacgac
     4861 attaattaag tcatcaacac caacatcaaa ggctttacca aatcctttta caaataagcc
     4921 tgccgttggc ttaaagctaa agagcttgaa atcttgcata tttgacagtt catcaactaa
     4981 atcgccgtga cgagctttaa gtgctgcaat gctattattc cattcctcat cagtacgttc
     5041 aatcattcgc actgttgcgt caaaggttaa gcgacgacgg gcaaaaattt gacggctctt
     5101 actttcatcc tcaattagca ttaaagagac ttttggcacc tcttgtaaat tacgagcgtg
     5161 acgggcaata gtagtgatta agacttgata ttcgccgtta ttaattacaa aaggggcata
     5221 gctcacattc ggtacaccat ctttatctat ggttgcaagc attatagttt tgatttgttg
     5281 ttttaattct tcaatttctg gacctaaac
//
LOCUS       DANLUQ010000155         5209 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.156, whole genome shotgun sequence.
ACCESSION   DANLUQ010000155 DANLUQ010000000
VERSION     DANLUQ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5209
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.156"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..222)
                     /locus_tag="PXB27_002204"
     CDS             complement(<1..222)
                     /locus_tag="PXB27_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346948.1"
                     /translation="MSSIKEIFSTKNSPINVLMQTEWFLGFILIVVYLDAYFQLVHQV
                     PIYIAIQQKEIYLTKTIDYIAAIFFFSVTF"
     gene            complement(292..1425)
                     /locus_tag="PXB27_002205"
     CDS             complement(292..1425)
                     /locus_tag="PXB27_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL5346949.1"
                     /translation="MKTIKIGLVGTGYIGRCHAIAYAQAPTLFPLKGKIQLEYLAEIN
                     QALADQKAREFGFQRATDDWRKLVTDPNVDVVDICTPNFLHKEIALEAIKHGKHVYSE
                     KPLALTAQDAKEMVIAAQQAGVKTLVGFNYIKNPTTQLARQIIQNGEIGEVVHFYGTH
                     NEDYLANPNTPIDWHCYKAKAGLGTLGDLAAHIVSIAHYLVGSDIERVIGDMQTVIEE
                     RPSPHNPAEKIKVENEDQASSLIRFANGVMGTLESSRISCGRKMGLTYVVTGTKGTLS
                     YTQERMAELKLYLHDDDKSRQGFKTILVGPEHPDYAAFCVSAGHGIGFNDQKAVEIRD
                     LINGIAADDPMYPDFEEGYKISRVLEAIAKSAEEKRWVEVSEI"
     gene            complement(1556..2584)
                     /locus_tag="PXB27_002206"
     CDS             complement(1556..2584)
                     /locus_tag="PXB27_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5346950.1"
                     /translation="MTTQFLKNMGMVDDKGKIDFIAFFERFGVLIFLILLIIFFTSQN
                     GAFLSQRNIYNVLTEVSIFGIMAVGMTFVILTAGIDLSVGSILAVTAMFAAYIIKGDN
                     TVTLEAGAWGGMSWLIGLSICLGLGTFIGWLHGLGVTKLKLPPFIITLGGMTIWRGLT
                     LVLNNGSPIAGFDEGYRWWGRGDILGVPVPVVIFAVVAIIGYFALHKTRWGRYVYSVG
                     GNPEAARLAGVNINRTLVSVYVVIGALAGLAGFILSARLGSAESVAGVSFELRVIASV
                     VIGGTSLMGGYGRVTGTIIGSIIMGVLINGLVLMDVSAYYQQIIMGLIIILAVAFDTY
                     AKSRRGAL"
     gene            complement(2596..4140)
                     /locus_tag="PXB27_002207"
     CDS             complement(2596..4140)
                     /locus_tag="PXB27_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL5346951.1"
                     /translation="MSTPLLEVKDLTKSFSGVMALNGVKLTVGKGEVHALLGENGAGK
                     STLLKALSGAQPQTSGEIIFNGETLSLSDSPFDRQLKGIVTIYQEFNLLPNMTVAENF
                     FLGREPVKGLFVNEKAVNEEAQLVLDNLGLNIKPDTQVSRLSVAQQQMVEIARAMTLN
                     AKLIIMDEPSAALSDKEVETLHQIVRDLKSRGISVIYVTHRLNEVFALCDRFTVFQDG
                     RYSGEGEVKNVNVDQIIKMMVGREVAFTRRPPEETHHTHRPIRLAVKNLHREKPAFDP
                     HGIALHGVSFNIHEGEVLGIAGLVGAGRTEIARCLFGVENYTADEISLDGKPYAATSP
                     LDALEKGVALVPEDRKKEGLVLGLPIKTNMTLPILSKLLKGNLFVDNQKEDDLIETYR
                     QALRIKMASSELEARKLSGGNQQKVILARCMALNPKVLIVDEPTRGIDVGAKSEVHQV
                     LFDMAKQGVAVLVISSDLPEIMAISDRIITLSEGRVTGEIHGDDATEEKLMSMMAVGV
                     QRKAAA"
     gene            complement(4191..5129)
                     /locus_tag="PXB27_002208"
     CDS             complement(4191..5129)
                     /locus_tag="PXB27_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDL5346952.1"
                     /translation="MKKRIKFVLSALTLGLAMNSFAKDELVVFSLPNLSSPFEVQLQK
                     VAVETSKKLEIKLQVLDGQSSSTKQASDLENAITRGAKGIIISPNDVNAISGAVEEII
                     KEKIPAATLDRKVESSKPVPHFGANNYTGGQEVAKAVKAKYPNGAKIILLTGQPGSTS
                     NIERTKGIRDELAAGGDKYKIVVDQTGNWLRSEGLRIIESVLPTLKEKPEVIISANDD
                     MALGAIEALRSQGLKAGDILVTGFDSTPEALARVKDGWLYLTADQRPSFAVSTALEQV
                     VGNIRESKEVSGADYPPKIILKDNLQEAERFAEISE"
BASE COUNT         1398 a         1226 c         1084 g         1501 t
ORIGIN      
        1 aaatgtaact gaaaaaaaga aaattgctgc aatataatca atggtttttg tgagatatat
       61 ttccttttgc tgtatcgcaa tatagatagg cacttgatga acaagttgga agtaagcatc
      121 caaataaaca actatcaata tgaagcctaa aaaccattct gtttgcataa gaacattgat
      181 agggctgttt tttgtgctaa aaatttcttt aattgaactc ataatttttc cttaaatgta
      241 aaaaaacgta cctcaaccaa tttgagatac gtttctattt taatgtaatt attaaatctc
      301 actcacctcc acccaccttt tctcctccgc cgattttgct atcgcttcca atactctgga
      361 gattttatag ccttcttcaa aatcgggata catcgggtca tcagcggcga tgccgttgat
      421 gaggtcacgg atttccaccg ctttttggtc gttaaaaccg atgccgtgac cggcactcac
      481 acaaaaagca gcgtagtcgg gatgttccgg gccgactaaa atggttttga atccttggcg
      541 ggatttatcg tcatcgtgca aatagagttt gagttctgcc attcgttctt gggtatagct
      601 gagcgtgcct ttagtacctg taacgacata ggtcagcccc attttacgcc cgcaggaaat
      661 gcgggagctt tcgagcgtgc ccattacccc attagcaaat cgtatgagac tacttgcctg
      721 atcttcgttt tctactttga ttttttctgc tggattgtgc ggactagggc gttcttcaat
      781 cactgtttgc atatcgccga taacacgctc aatgtctgaa ccaaccaaat agtgtgcgat
      841 actgacaata tgtgcggcta aatctcccaa cgtaccaagc ccggctttgg ctttgtagca
      901 gtgccaatca atcggggtat tagggttggc gagataatct tcgttatgcg tgccgtagaa
      961 atgcaccacc tcaccaattt cgccgttttg aataatttgg cgagccagtt gggtggttgg
     1021 atttttgata tagttgaaac cgaccaaggt tttaactccc gcttgttgag cggcaatcac
     1081 catttctttg gcatcttggg cggtgagtgc aagcggtttt tctgaataga cgtgtttgcc
     1141 gtgtttaatc gcttctaagg caatttcttt gtgcagaaaa tttggtgtac aaatgtccac
     1201 cacatcgaca ttcggatcgg taaccaattt tcgccaatcg tcagtagcac gctgaaagcc
     1261 aaattctcgg gctttttgat cggctaatgc ttggttgatt tcggcaaggt attccagttg
     1321 gattttgcct ttaagtggaa acagggttgg ggcttgagcg taggcaatgg cgtggcagcg
     1381 accgatatag ccggtgccaa ctaagccgat tttgatggtt ttcataatac tatcccagta
     1441 gggtgcgttt gctaacgcac cattagaatt ttcagtgcat tggcaaacgc accctataag
     1501 aaagcggtct ggttttgcaa aatttttaca aaatcagacc gcttgtgtta gctatctata
     1561 atgctcctcg tctgctcttc gcataggtgt cgaaggcaac ggcaaggatg atgattaagc
     1621 ccataatgat ttgttggtag taagcggaaa cgtccattag tactaaaccg ttaatcaata
     1681 cacccataat aatggagccg atgatggtgc ctgtgactcg tccgtatccc cccattaggg
     1741 aagttccgcc gataaccact gaggcaatta cacgtagctc aaaagaaacg ccggcaacgg
     1801 attcggcact gcctaaacgg gcggagagaa tgaaaccggc aagtcctgcc aatgctccga
     1861 tcaccacata aacactgact aaggtacggt taatattcac accggctaaa cgggctgctt
     1921 ccggattgcc acctaccgag tacacatagc gtccccagcg ggttttatgc aatgcgaagt
     1981 aaccgataat ggcgactacg gcaaaaatca ctaccggcac cggtacgcct aaaatatcgc
     2041 cacgtcccca ccagcggtaa ccttcgtcaa atccggcaat tggcgagccg ttatttaaca
     2101 caagtgttaa accacgccaa attgtcatac cgccaagcgt gataataaac ggtggcagtt
     2161 tgagtttagt gacgcctaag ccgtgtaacc aaccaatgaa agtgcctaaa cctaaacaga
     2221 tactcaaacc tatcagccag ctcattccgc cccaagcacc ggcttctagc gttaccgtat
     2281 tatcgccttt gataatatag gccgcgaaca ttgcggtaac agcaaggatt gagccgacag
     2341 agagatcgat ccctgcagtt aagatgacga aagtcattcc taccgccata atgccgaaaa
     2401 tcgacacttc ggttaaaacg ttatagatgt tgcgttgcga taaaaatgcc ccgttttgtg
     2461 aagtgaagaa aataatcagt aagatcaaga aaatcagtac gccgaaacgt tcaaagaagg
     2521 cgataaaatc gatttttcct ttgtcatcga ccatacccat attttttaga aattgcgttg
     2581 tcatacaaac ctctcttatg ctgccgcttt acgctgaacg ccaacagcca tcattgacat
     2641 taatttttct tcggtggcat catcgccgtg aatttcgcca gttacccgac cttcggaaag
     2701 agtgataatg cggtcggaaa tcgccataat ttccggtaaa tcggaggaaa tcactaatac
     2761 cgcaacacct tgttttgcca tatcaaacag cacttggtga acctcagatt ttgccccaac
     2821 atcaatacct cgtgtcggct catcaacaat tagtactttc gggttgagtg ccatacaacg
     2881 ggctaaaatc actttttgct gattaccacc cgataatttg cgggcttcaa gttcgctact
     2941 tgccattttg atgcgtaacg cttggcggta ggtttcgatt aaatcatctt ctttttgatt
     3001 atcgacaaac aagttaccct tcaataattt gcttaaaatc ggcagggtca tattggtttt
     3061 aatcggtaaa ccgagaacta agccctcttt tttgcgatct tccggcacaa gggcaacccc
     3121 tttttccaaa gcatctaacg gcgaggtggc agcataaggc ttaccgtcta ggctgatttc
     3181 atcagccgta taattttcta ccccaaacaa gcaacgggca atttcagttc gccctgcgcc
     3241 aactaagccg gcgatcccta atacttcacc ctcgtgaatg ttaaaagaga caccgtggag
     3301 agcaatgccg tgtggatcga atgccggttt ttcccgatgc agatttttca cagctaaacg
     3361 tattggtcgg tgggtatggt gagtctcttc tggtggacgg cgagtaaagg caacttcacg
     3421 tcctaccatc attttaataa tttgatcgac attcacattt ttgacttcac cctcaccaga
     3481 atagcgcccg tcttggaaaa cggtgaaacg gtcgcatagg gcaaacactt cgtttaagcg
     3541 gtgggtcaca taaatcacgc taatgccacg gctttttaaa tcacgcacga tttggtgtag
     3601 tgtttccact tctttatcac ttaaggcagc ggacggttcg tccataataa tcaacttagc
     3661 gtttaaggtc attgcacggg caatctcgac catctgctgt tgtgccacac ttaaacggga
     3721 aacttgggta tcaggtttga tatttaagcc gagattatcc agcactaatt gtgcttcctc
     3781 attcaccgct ttttcattga cgaataaacc tttcaccggc tctctgccca aaaagaaatt
     3841 ttcagcaacc gtcatattcg gtaacaagtt aaattcttga taaatcgtta cgatgccttt
     3901 gagttggcga tcaaacgggg aatcgctcaa acttaaggtt tcaccgttaa agataatttc
     3961 gccacttgtt tgtggctgag cgcctgaaag tgcttttaat aaggtcgatt ttcctgcacc
     4021 gttttcaccc aacaatgcgt gaacttcgcc ttttcccaca gtcagtttca cgccattgag
     4081 tgccataaca ccagagaaac tttttgtcag atctttcact tcaagtaagg gagtagacat
     4141 acaacctccg taaaaaaacg ccctccggcc tcagccggaa ggcagataaa ttattcgctg
     4201 atttctgcaa aacgttctgc ttcttgcaga ttatctttca ggataatttt tggcggatag
     4261 tccgcaccgc tcacttcttt gctctcacgg atattgccaa ccacttgctc taatgcggtg
     4321 cttaccgcaa aacttgggcg ttggtcagcg gtgagataca accagccatc tttcacacgt
     4381 gcaagagcct caggggttga gtcaaagccg gttactaaaa tatcgccggc tttcaaacct
     4441 tggctacgca atgcttcaat cgcaccaagt gccatatcgt cattcgctga aataatcact
     4501 tctggtttct cttttaaggt tggtaacaca ctttcaataa tgcgtaatcc ctcagaacgg
     4561 agccagttac cggtttgatc aaccacgatt ttatatttgt caccaccggc agctaattca
     4621 tcacgaatac ctttggtacg ttcaatgtta gaggttgaac ccggctggcc ggttaaaaga
     4681 ataatttttg ccccattcgg gtatttggct ttcactgctt ttgcgacttc ttgaccgcct
     4741 gtgtagttat ttgccccaaa gtgtggaacc ggcttactgc tttcaacttt tcggtctaat
     4801 gtcgcagcag gaattttttc tttgataatt tcttccactg cacctgaaat cgcattcaca
     4861 tcattcgggg aaatgataat gcctttagca ccacgggtaa ttgcattttc taagtcagaa
     4921 gcctgcttag tagaagaact ttgcccgtct aatacttgca atttgatctc caacttttta
     4981 ctggtttcta ccgctacttt ttgtagttga acttcaaacg ggctggatag gttcggtaag
     5041 ctaaatacca caagctcatc tttcgcaaag ctgttcattg ctaagcctaa cgttaaagca
     5101 ctcaatacaa atttaatacg ctttttcatt acaaaatcct ctttttaaga tgggaattga
     5161 cggcgaatat tgccaatgtt acttcaaact gcgaaggaaa cttaagtct
//
LOCUS       DANLUQ010000156         5194 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.157, whole genome shotgun sequence.
ACCESSION   DANLUQ010000156 DANLUQ010000000
VERSION     DANLUQ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5194
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.157"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..636
                     /locus_tag="PXB27_002209"
     CDS             <1..636
                     /locus_tag="PXB27_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase family
                     protein"
                     /protein_id="HDL5346953.1"
                     /translation="TTVFPKQVHFADYLNYAIFADFLEKNQVNYNLNIPNELSSFANT
                     LWQEKKIRLLLNPQGSMREISTQELSELLSMIDKRYVQHISCLLTNTTNSAEYLSKIK
                     APFEISLAPNSDILGYCALVNSADIVIAVDGGGVHISCALGKKLLSFYANHPANIARW
                     YPKPKKDVETFMVIADKWTEDNNDTRGFSLVAPAKWLNHQFAKLIEKYTAC"
     gene            complement(691..903)
                     /gene="rpmE"
                     /locus_tag="PXB27_002210"
     CDS             complement(691..903)
                     /gene="rpmE"
                     /locus_tag="PXB27_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005625828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HDL5346954.1"
                     /translation="MKQGIHPEYKEITATCSCGNVVKTRSTVGKDLNLDVCGNCHPFY
                     TGKQRVVDTGGRVERFNKRFSIPSSK"
     gene            1266..3272
                     /gene="tkt"
                     /locus_tag="PXB27_002211"
     CDS             1266..3272
                     /gene="tkt"
                     /locus_tag="PXB27_002211"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDL5346955.1"
                     /translation="MTERKVLANAIRFLSMDAVQKANSGHPGAPMGMADIAEVLWRDF
                     LKHNPTNPKWADRDRFVLSNGHGSMLIYSLLHLTGYDLSIEDLKQFRQLHSKTPGHPE
                     YGYAPGVETTTGPLGQGITNAVGIAIAEKTLAAQFNREGHKIVDHYTYAFLGDGCLME
                     GISHEACSLAGTLGLGKLIAFYDDNNISIDGHVDGWFTDDTAQRFESYGWQVIRNVDG
                     HNPEQIKFAIENAQAETERPTLIICKTIIGYGSPNKSNSHDCHGAPLGDAEIQAAREF
                     LGWEHAPFVIPAEIYTEWDAKAKGTLAEKDWNAKFAAYEAAYPELAAEFKRRYAGELP
                     ANWEAESKAFIEKLQANPATIASRKASQNAIEAYAPILPEFLGGSADLASSNLTLWSG
                     SKPIRAEHNVDGNYINYGVREFGMSAIMNGIALHGGFIPYGATFLMFYEYAHNAVRMA
                     ALMKQRALFVYTHDSIGLGEDGPTHQPVEQTASLRQIPNLETWRPADQVESAVAWKAA
                     VERKDGPTALIFTRQNLAQQQRTAEQLANISRGGYILRECCEKGSCPDLILIATGSEV
                     DLAMKAADVLDAEGVKVRVVSMPSTNVFDKQDEAYRESVLPRSVTKRVAIEAQLADFW
                     YKYVGFDGRIVGMNSFGESAPADQLFKLFGFTVENVVAKAKEIL"
     gene            complement(3398..4468)
                     /locus_tag="PXB27_002212"
     CDS             complement(3398..4468)
                     /locus_tag="PXB27_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDL5346956.1"
                     /translation="MNEIIFSLEPQDNARLQSLCGAFDEHLALIEKSFNLIIARTGFN
                     FTIQSEDDNHYSATLVQNSVKLIKQLYNETAPIKGKIKELDLEDIHLAIQESRMLLQN
                     QWQAGSQGEIAIKTKRGVIKPRSEHQQAYLRNILSHDISFGIGPAGTGKTFLAVAAAV
                     ESLERQEIRRILLTRPAVEAGEKLGFLPGDLGQKIEPYLRPLYDALFEMLGFEKAQKL
                     MERGVIEIAPLAYMRGRTLNDAFIILDESQNTTTEQMKMFLTRIGFNSKAVITGDITQ
                     VDLPRSQKSGLKHAMEVLKEVPELSFNFFDSKDIVRHPVVAKIVQAYDAWEAVDEERK
                     EQRKLEKLALEQEKILAQAEML"
     gene            complement(4483..>5194)
                     /gene="fadR"
                     /locus_tag="PXB27_002213"
     CDS             complement(4483..>5194)
                     /gene="fadR"
                     /locus_tag="PXB27_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HDL5346957.1"
                     /translation="ILKAQSPAALAEEYIVKSIWNNHFPAGSDLPAERELAERIGVTR
                     TTLREVLQRLAREGWLSIQHGKPTRVNDIWETSGPNIIGTIIKLDKSYLPVVIANVVS
                     LRTRMAEAYIPEAIYLAPEESAKLFDGLDELENTAAAYAEFDYKLFRQFTFLANKPVY
                     ALILNSFKSMYHQVAKIFFADENNRQLTLSFYRSLLCTCLAKDSEKAAACMAKNRQSS
                     GEIWQKLSANIPESFGQL"
BASE COUNT         1556 a         1080 c         1017 g         1541 t
ORIGIN      
        1 accactgtct ttccaaagca ggttcatttt gctgactatc tcaactatgc tatttttgct
       61 gattttttag aaaaaaatca ggtcaattat aatttaaaca ttcctaatga actaagcagt
      121 tttgcaaata cgctatggca agagaagaaa attcgattac tattgaatcc tcaaggtagt
      181 atgcgtgaaa tttctactca agaattatca gagctattat ctatgattga taaaagatat
      241 gttcaacata ttagttgttt gctgaccaat acaaccaata gtgccgaata tttgtcgaaa
      301 attaaagccc catttgaaat aagcctagca cctaatagtg atattttagg ttattgtgca
      361 ttagtaaact ctgctgatat tgtaattgca gttgatggag gtggtgtcca tatttcttgt
      421 gcattaggca agaaattgct ctcattttat gccaatcatc cagctaatat tgcacgctgg
      481 tatccaaaac ctaagaaaga tgttgaaact tttatggtga tcgcagataa atggaccgag
      541 gataataatg atactcgagg tttttctctg gtagctcctg caaaatggtt aaatcaccaa
      601 tttgcaaaat tgatagaaaa atacaccgct tgttaattcg atatataaga aaaacaaaac
      661 cccgcattag cggggtttgc aataatcaaa ttattttgag cttgggatgc tgaaacgttt
      721 gttaaagcgt tcaacacgac caccggtgtc aacaacacgt tgtttaccag tatagaacgg
      781 gtggcagttg ccacatacgt ctaagtttaa atctttaccc actgttgagc gagttttaac
      841 tacattaccg caagagcaag ttgccgtaat ttctttatat tctgggtgaa taccttgttt
      901 cattggggaa cctcataaga agccatatcg ccgcctatct tgttagaatc tctaccgctg
      961 ataggtacca tatgtaatta ataaaaatgt tgagcaaatg ctcaacgcca aaaagagtgc
     1021 gtattttaga taaatttgtt gcctagatca actactaaac attcgcttta tcaagaaaat
     1081 attgtattag gaaaaaaccg tctattaaaa tcaaatccaa tcaaaacaaa tcacaattta
     1141 atcatttttt gtgatacaga tcacatttct catttccatt tttgagtttt tctagcaaaa
     1201 tagtgtagaa tccaattcga tatcctttcc attttatata acattctcga ttaggagtaa
     1261 cacaaatgac agaacgtaaa gtgctagcga atgcaattcg ttttttaagt atggatgctg
     1321 tacaaaaagc taactcaggc cacccaggcg caccaatggg tatggctgat attgcagaag
     1381 ttttatggcg tgatttttta aaacacaatc caactaatcc taaatgggca gatcgtgacc
     1441 gttttgttct gtctaacgga cacggttcaa tgttaatcta tagcttactt catttaaccg
     1501 gctatgatct ctctattgaa gatttaaaac agttccgtca gttacattct aaaacacctg
     1561 gtcaccctga atatggttat gcaccgggtg ttgaaaccac cacaggcccg ttaggtcaag
     1621 gtattaccaa tgcggtcggt atagcgattg cggaaaaaac ccttgcagca caatttaacc
     1681 gtgaaggtca taaaattgtc gatcactaca cttacgcttt cttaggcgat ggctgtttaa
     1741 tggaaggtat ctcacacgaa gcttgctctc tcgccggtac attaggctta ggcaaactga
     1801 ttgctttcta cgatgataac aatatctcta tcgacggcca cgttgatggt tggttcactg
     1861 atgataccgc acaacgcttt gagtcttatg gctggcaagt tatccgtaat gtcgatggcc
     1921 ataacccaga acaaatcaaa tttgcgattg aaaatgcaca agccgaaacc gagcgtccaa
     1981 ctttaattat ttgtaaaacc attatcggct atggttcacc aaataaatca aactctcacg
     2041 attgccacgg tgcgccattg ggtgatgctg aaatccaagc cgctcgtgaa tttttaggtt
     2101 gggaacatgc cccatttgtg attccagctg aaatttatac agagtgggat gcaaaagcaa
     2161 aaggtacatt agcagaaaaa gattggaacg ctaaatttgc tgcttatgaa gcggcatacc
     2221 ctgaactagc cgctgaattt aaacgccgtt acgcaggcga attaccggct aactgggaag
     2281 ctgaaagcaa agcttttatt gaaaaattac aagcaaatcc ggcaaccatt gcaagccgta
     2341 aagcatcaca aaatgcaatc gaagcttacg cgccaatctt acctgaattc ctcggcggct
     2401 cagctgactt agcaagctct aacttaacct tatggtctgg ctcaaaaccg attcgtgcgg
     2461 aacacaatgt agatggtaac tacatcaact acggcgtgcg tgaattcggt atgtctgcga
     2521 ttatgaacgg gattgcccta cacggtggtt tcattcctta cggtgcaacc ttcttaatgt
     2581 tctacgaata tgcacacaat gcggtgcgta tggcagcctt aatgaagcaa cgtgccttat
     2641 ttgtttatac acacgactcc atcggtttag gtgaagacgg cccgacccac caaccggtag
     2701 aacaaactgc ttcattacgc caaattccaa acttagaaac ctggcgtccg gcagaccaag
     2761 tagaatctgc tgttgcttgg aaagcggcgg tagaacgtaa agacggcccg acagcactaa
     2821 tctttacccg ccaaaatctg gctcaacaac aacgtactgc ggagcaatta gcgaacatct
     2881 ctcgtggtgg ctatatcttg cgtgaatgtt gcgaaaaagg cagttgcccg gatttaatct
     2941 taattgcaac cggttctgaa gtagatttag caatgaaagc cgctgatgtg ttagatgcag
     3001 aaggcgtgaa agtgcgtgta gtttcaatgc caagcaccaa cgtgtttgac aaacaagatg
     3061 aagcctatcg tgaatcggta ttaccacgtt cagtcactaa acgtgtggcg attgaagctc
     3121 aattagctga cttctggtat aaatatgtcg gctttgatgg tcgaattgta ggtatgaata
     3181 gctttggtga atccgcacca gcagatcaat tattcaaact cttcggcttt accgttgaaa
     3241 acgtggttgc taaagcaaaa gaaatcttat aatgaaacaa taagataaag aaaagcggga
     3301 attaacttcc cgcttttttg ttgaacaact gttatgataa gcggtgaaat ttcagcgatt
     3361 ttttgcaaaa aattagcgaa atatgaccgc ttgtaagcta taacatttca gcttgagcaa
     3421 gaattttttc ttgctctaaa gctaattttt ccagttttcg ctgttcttta cgctcttcat
     3481 caaccgcttc ccacgcatca taggcttgca caattttggc aaccacaggg tggcggacaa
     3541 tatctttgct gtcaaagaaa ttaaagctga gttccggcac ttcttttagc acttccattg
     3601 cgtgttttaa gcctgatttt tggctacggg gcaaatccac ttgggtaata tcgccggtaa
     3661 tcaccgcttt ggaattaaag ccaatacggg ttaagaacat tttcatttgc tcggtagtcg
     3721 tgttttggct ttcatctaaa atgatgaacg catcgtttaa agtacgccct cgcatataag
     3781 caagtggggc aatttcaatc acaccacgtt ccatcaactt ttgagctttt tcaaagccaa
     3841 gcatttcaaa cagggcatca tataacgggc gtaagtaagg ctcaattttt tgccctaaat
     3901 cgccgggtaa aaaaccgagc ttttcaccgg cttccaccgc cggacgggta agtaaaatac
     3961 ggcggatttc ttggcgttca agcgattcca ccgctgccgc taccgctaaa aaggttttgc
     4021 ctgtgccagc aggcccgata ccaaaactga tgtcgtggct taatatattt cgtaaatagg
     4081 cttgttgatg ttcgctacga ggtttaatca cccctcgctt ggttttaatc gctatttcgc
     4141 cttgcgagcc ggcttgccat tggttttgta gtagcattcg gctttcctga atcgccaaat
     4201 gtatatcttc taagtccaat tccttaattt tgcctttaat cggggcagtt tcattataca
     4261 gttgcttaat cagcttcaca ctattttgaa ccaaggtagc agaatagtga ttgtcatctt
     4321 ccgattgaat cgtaaagttg aagcctgttc gagcaataat gagattaaaa cttttttcaa
     4381 ttaaggctaa atgctcatca aatgcaccac aaagcgattg cagacgagca ttatcttgcg
     4441 gttctaagct aaaaataatt tcgttcaaaa ttctaccttc tgttaaagct gaccaaagct
     4501 ttcaggaata ttagcggata atttttgcca aatttcacca ctagattggc gatttttagc
     4561 catacaagcg gctgcttttt cactatcttt tgcaagacaa gtacataata atgaacggta
     4621 aaagcttaac gttaattgac gattattttc atcagcaaag aaaatttttg cgacttgatg
     4681 atacattgat ttaaagctat ttaggatcaa cgcatacaca ggcttattgg ctaaaaaagt
     4741 aaattgtctg aataacttat aatcaaattc agcataagca gctgctgtat tttctaattc
     4801 atctagtcca tcaaagagtt tagcactttc ttcaggagca aggtaaatcg cttctggaat
     4861 ataagcctcc gccatacgag tacgtaatga caccacattg gcaatcacca ctggaagata
     4921 gcttttatct agcttaataa tagtgccaat aatattaggc cccgaagttt cccaaatatc
     4981 attcactctt gttggcttgc cgtgctgaat acttaaccaa ccttctcgcg ctagacgttg
     5041 tagtacttca cgtaaagttg ttcttgttac cccaatgcgc tctgctaact ctcgctcggc
     5101 aggaagatca gagccggcag ggaaatgatt attccaaata ctctttacaa tatattcttc
     5161 cgctaacgct gccgggcttt gcgcttttaa aata
//
LOCUS       DANLUQ010000157         5163 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.158, whole genome shotgun sequence.
ACCESSION   DANLUQ010000157 DANLUQ010000000
VERSION     DANLUQ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.158"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            163..624
                     /locus_tag="PXB27_002214"
     CDS             163..624
                     /locus_tag="PXB27_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HDL5346958.1"
                     /translation="MTACPCQTGKRYAECCQPFHLKQAFASTAEQLMRSRYSAYTLIN
                     IPYIVETTVPSQQKLLDQTAMKEWAENTKWVGLSVLNHLPSLSKIHSQVEFKAFFDTD
                     EGIQAHHETSLFVQIDKRWFFVDPTVPLPHQKQPCVCGSGKKFKHCCGNYL"
     gene            696..1325
                     /locus_tag="PXB27_002215"
     CDS             696..1325
                     /locus_tag="PXB27_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HDL5346959.1"
                     /translation="MDFSKILSQVLDTAKDAATNGLIKGNSKNDQIAKIGGGAAAIGL
                     ISMLFGRNGGSSLAKLGSLAALGSLAYQAYQKYQAQNASASELSQNSFENVDKNVASK
                     VILQAMIAAAASDGAITEEEKAAIISQVGDDVEVIEWIRQEMDSPASVEEIARQVGND
                     QALATQVYLAARAVCTNLERKEIVFLANLAEALGLAEALVEQLEKDAGF"
     gene            complement(1408..2055)
                     /locus_tag="PXB27_002216"
     CDS             complement(1408..2055)
                     /locus_tag="PXB27_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015702020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5346960.1"
                     /translation="MISIRNVSKQYRTNNGWKTILKDINFELKKGEKVGILGRNGAGK
                     STLIRLMSGVEPPSTGTIERQMSISWPLAFSGAFQGSLTGMDNLRFVSRIYGADFDYV
                     KSFTEEFSELGEYLYEPVKNYSSGMKARLAFALSLSIEFDCYLIDEVIAVGDARFAAK
                     CKYELFEKRKDRSLILVSHSPASIKEYCDSAMVLEEGIMYTFDSIDNAYNFYNKT"
     gene            complement(2052..2849)
                     /locus_tag="PXB27_002217"
     CDS             complement(2052..2849)
                     /locus_tag="PXB27_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157140410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL5346961.1"
                     /translation="MRYGDNTTFKRSCVIQKRVIHALLMRELITRYGRKNIGFLWLFI
                     EPLLLTLVITVAWKYIKADKFSTLNIIAFMITGYPIAMMWRNASNRAQGAISANLSLL
                     YHRNVGVLDTIFTRVLLEVTGASIAQILLMTIFIAIGWIEMPNDIFYMLCAWILMAFF
                     ALGLGLIICTLAHYIEPFGKIWGTFSFFLLPLSGAFFFVHNLPLTAQNIALWFPMIHG
                     TEMFRHGYFGETVITYESISYLIVSDIILLFIGLLCVRSFSKGIEPR"
     gene            complement(2849..3952)
                     /locus_tag="PXB27_002218"
     CDS             complement(2849..3952)
                     /locus_tag="PXB27_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HDL5346962.1"
                     /translation="MSKKKKHFLANPLLWLTVIIPTACSGIYYSLFASDIYVSESSFV
                     VRSPNNQSSLTGVGALLQNSGFARSQDDTYSVQEYMRSRTALEQLEAEIPVKRFYSEQ
                     GDLLSKFNGLGFNDTQEAFYRYFKERLNIDVDSISGIATLRVRAFNAEEGQKINENLL
                     QQGETLINRLNERARQDTVEFAEERVKEAEKDVNNTALALSQYRTKNKIYDLPAQSSV
                     QLSLISTLKSELIRVETQLAQLKSITPDNPQVAALQMRQKSLKKEIDDQSKQLAGGNT
                     SVATQTAEYQRLVLANELAQQQLAAAMTSLQNTRGEADRQQLYLEVIGRPSKPDWALE
                     PYRIYNIISTFFIGLILFGVLSLLLASIREHKN"
     gene            complement(4024..>5163)
                     /locus_tag="PXB27_002219"
     CDS             complement(4024..>5163)
                     /locus_tag="PXB27_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015702017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HDL5346963.1"
                     /translation="SLISACSSLPTSGPSYSEILASNENTAETQLPEVNLIKLDNITV
                     QNLYQEQQSQRFSGFDGSMGVGGYAGTVNVGDILEISIWEAPPAVLFGGTFSAEGQGN
                     GHLTQLPQQMVNKNGTVTVPFIGNIKVAGKTPEVIQNQIVGALSRKANQPQALVRIAN
                     NLSSDVTVIRQGNSIRMPLSANNERILDAVAAVGGTSENIEDVTVRLTRGNKVRSMAF
                     ETLIADPSQNITLRSGDVIALMNTPYSFTGLGAVGNNQQMRFSSKGITLAEAIGKMGG
                     LIDERSDPRGVFVFRHIPFTQLDYTSQAHWREKGYYEGMDIPTVYQVNLLEPQSIFLL
                     QRFPIQDKDIVYVSNAPLSEFQKFLRMIFSITTPITGTANTIRAY"
BASE COUNT         1633 a          968 c          917 g         1645 t
ORIGIN      
        1 aggcttttta aagcaatgaa tacccaaaaa attcacgctt tttgcataag ttatccacaa
       61 ggggtatgtc acgctttttt ataaaattta tttttcttta tttatagcgt aaacttttca
      121 catctttatt tttatgcgga aatgtaaaaa tgttagaaaa aaatcaccgc ttgtccttgc
      181 caaacaggca aacgctacgc tgaatgttgc caacctttcc atttaaaaca ggcatttgca
      241 tcaacggcag agcaattaat gcgttcccgt tacagtgctt atactttgat taatattccc
      301 tatattgtgg aaacgacagt gccaagtcag cagaaattac ttgatcagac tgcaatgaaa
      361 gagtgggctg aaaatacaaa atgggtagga ttatccgtgc taaatcactt accttctctt
      421 tcgaaaatac atagtcaggt tgaatttaaa gccttttttg atacagatga ggggatacag
      481 gcacaccacg aaacctctct ttttgttcaa attgataagc gatggttttt tgttgatccc
      541 accgtacctt tgcctcatca aaaacaacct tgtgtatgtg gttccggaaa gaagtttaag
      601 cactgttgtg gaaattatct ttaatttgaa ttgaactctt gtttgaatct aaggactaat
      661 gagtaaattt catttactta ccaagaggaa caaaaatgga tttcagtaag attttaagtc
      721 aagtattaga tacagcaaaa gatgctgcta caaacggatt aattaaaggt aattctaaaa
      781 acgatcaaat cgctaaaatt ggtggcggcg ctgccgctat tggtttaatt tcaatgttat
      841 ttgggcgtaa tggcggttca agtttagcga aattaggctc tttagctgct ttaggtagcc
      901 ttgcttatca ggcatatcaa aaataccaag cacaaaatgc atcagcatca gagttaagcc
      961 aaaatagttt tgagaatgtt gataaaaatg tagcaagtaa agtgatttta caagcaatga
     1021 ttgccgctgc cgcttcagat ggtgctatta ctgaagagga aaaagcagct atcatttctc
     1081 aagttggaga tgatgtagaa gtaattgagt ggattcgtca agaaatggat tccccagctt
     1141 cagtcgaaga gattgctcgt caagtaggta atgatcaagc attagctact caagtttact
     1201 tagctgcccg ggcagtatgt accaatttag aacgcaaaga aatagttttc ttagctaatc
     1261 tagctgaagc tctagggtta gcagaggctt tagtggaaca attagaaaaa gatgcaggct
     1321 tttaattttg cttattttta gtaacaagcg gttatatttt gagagttttt tgcgaaaaac
     1381 ttgtgaaata tgaccgcttg ttctttttta tgttttattg taaaaattgt atgcgttatc
     1441 aatagaatca aatgtataca tgataccctc ttctaaaacc attgcagaat cgcaatattc
     1501 tttaattgag gctgggctat gagaaactaa aatcaatgaa cggtctttcc gtttttcaaa
     1561 aagctcatac ttgcattttg ctgcaaatcg ggcatcgccc acagcaatta cttcgtcaat
     1621 taaataacaa tcaaattcaa tagagagaga taaagcaaaa gcgagtcttg ctttcatacc
     1681 agaagaatag tttttgaccg gctcatatag gtattccccg agttcagaaa attcttcggt
     1741 aaatgatttt acataatcaa aatctgcacc ataaattcta cttacaaacc gtaggttatc
     1801 cataccggtt aaacttcctt ggaaagcacc gctaaacgct aggggccaag aaatagacat
     1861 ttggcgttct attgtgcctg ttgatggggg ttctaccccg ctcattaaac ggattaaggt
     1921 tgattttcct gcaccattcc gccctaaaat ccccactttt tcgccttttt tcagctcaaa
     1981 atttatatct tttaatatgg ttttccaacc gttattagtt cgatattgct tacttacatt
     2041 tctaatacta atcatcttgg ttctatccct ttactaaaac ttctcacaca aagcagtcct
     2101 ataaatagaa gaataatatc acttactata agataactaa tgctctcata ggtaattacc
     2161 gtttcaccaa aataaccatg tctaaacatt tctgtaccat gaatcattgg aaaccataag
     2221 gcaatatttt gagcagttag cggtaaatta tggacaaaga agaatgcacc agaaagagga
     2281 agtagaaaga agctgaatgt tccccaaatc ttaccaaaag gttcaatata atgtgctaaa
     2341 gtacagataa ttaaacctaa acctaaggca aaaaatgcca ttaatatcca agcacagagc
     2401 atatagaaaa tatcgttagg catttctatc caaccaatag cgataaatat agtcattaag
     2461 agaatttggg cgatagatgc gccagtaact tctaataaaa ctcgagtaaa tatcgtatct
     2521 agcactccca catttctgtg gtaaagcaaa cttaagtttg ctgatattgc tccttgagct
     2581 ctattagagg catttctcca catcattgcg atagggtagc ctgtaatcat aaaagcgata
     2641 atatttaaag ttgaaaactt atctgctttt atatatttcc aagcaacagt aatcactaac
     2701 gttaacaata atggttcgat aaaaagccat aagaagccaa tatttttacg cccataacga
     2761 gtaattaatt ccctcattaa aagggcgtga atcacccgtt tttgaatcac acaggaacgt
     2821 ttaaaggttg tattatcacc atatcgcatt agtttttatg ctctctaata cttgctagta
     2881 acaaacttaa tactccaaat agaattaagc cgataaaaaa agttgatata atattataaa
     2941 ttctgtaggg ttctaatgcc caatctggtt tgcttggcct accaattacc tctaaataaa
     3001 gttgttggcg atcagcttca cctcgtgtat tttgtaatga agtcattgct gctgcaagtt
     3061 gttgctgagc taattcgtta gcaagaacca aacgttgata ttcagcagtt tgcgttgcta
     3121 cagaagtatt acctcctgca agttgtttgg actgatcatc aatttctttt ttcaaacttt
     3181 tttgacgcat ttgtaatgcg gctacctgag gattatccgg cgtaattgac ttcaattgtg
     3241 ctaattgtgt ttctacccga attaattcac tttttaaggt agaaattaat gagagctgaa
     3301 cgctggattg tgccggtaaa tcataaattt tatttttagt tcgatattgg cttaatgcta
     3361 acgcagtatt attgacgtct ttttcggcct cttttacacg ctcttctgca aactcaaccg
     3421 tatcttgtct tgctctttca ttaaggcggt taattaatgt ttccccttgc tgtaataaat
     3481 tctcgttaat tttttgtcct tcttctgcat taaaagcacg aacgcgtagc gttgcaatac
     3541 ctgaaataga atcgacatca atattcagac gttctttaaa ataacgataa aatgcttctt
     3601 gggtatcgtt aaatcctaaa ccgttaaatt tgcttagtaa atcaccttgc tcactataaa
     3661 aacgctttac cggaatttct gcttcaagtt gttctaatgc ggtacgggaa cgcatatatt
     3721 cctgaactga ataggtatca tcttgagaac gggcgaaacc ggagttttgc aataaagcac
     3781 caacgcctgt taagctcgat tgattattgg gcgaacgaac cacaaagcta gattctgaaa
     3841 cataaatatc agaggcaaat aaggaataat aaattccaga acaagcagtt ggaataatta
     3901 cagttagcca caacaaaggg ttagctaaaa aatgtttttt cttctttgac ataaaatcct
     3961 caacgaaaag tgttacaagc ggtcattttt taatgatttt ttaccatttt atctatgagc
     4021 taattagtat gctctaattg tattagctgt acctgtaatt ggcgttgtaa tggagaaaat
     4081 cattctcaag aatttttgaa attcagacag tggagcattc gacacataca caatatcttt
     4141 atcttgaata gggaaacgct gtaataaaaa aattgattgt ggttctaata aatttacctg
     4201 ataaacggtt ggaatgtcca ttccctcgta atagcctttt tctcgccaat gagcttgact
     4261 agtatagtct aactgggtga aaggaatatg gcggaatacg aacacaccac gagggtctga
     4321 acgctcatca attaatcctc ccattttacc gatagcttca gcaagggtaa tgcctttact
     4381 tgaaaaacgc atttgttgat tattacctac tgctcctaat ccggtaaagc tataaggagt
     4441 attcattaaa gcaataacat cacctgaacg taatgtaata ttttgtgagg ggtcagcaat
     4501 taatgtttca aacgccattg aacgcacttt atttccacgt gttaaacgaa ctgttacatc
     4561 ttcaatattt tcgcttgtac cacctactgc cgcaactgca tctaaaatac gctcattatt
     4621 ggcacttaat ggcatacgaa tgctatttcc ttggcggata accgtaacat cagacgacaa
     4681 gttattcgca atacgaacta atgcttgagg ttgattagct ttacggctca atgctccgac
     4741 aatttgattc tgaattactt caggtgtttt tccagcaact ttaatattac cgataaaagg
     4801 cacagttacc gttccatttt tgttcaccat ttgctgaggt aattgggtta aatgcccatt
     4861 accttgccct tcagcactaa atgttccacc aaatagcacg gcaggtggcg cttcccaaat
     4921 ggatatttct aatatatcgc ctacattaac tgttccagca taaccaccca cacccattga
     4981 gccatcaaat cctgaaaaac gttgagattg ttgctcttgg tataagtttt gtacggtgat
     5041 gttatctaac ttgattaaat tgacttcagg caactgcgtt tcagctgtat tttcattact
     5101 tgctaagatt tcgctatagc ttgggccgga cgttggtagg ctagagcagg ctgaaattaa
     5161 tga
//
LOCUS       DANLUQ010000158         5140 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.159, whole genome shotgun sequence.
ACCESSION   DANLUQ010000158 DANLUQ010000000
VERSION     DANLUQ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5140
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.159"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..648
                     /locus_tag="PXB27_002220"
     CDS             <1..648
                     /locus_tag="PXB27_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL5346964.1"
                     /translation="EYPKNLFKGLMVRPDKPKKKLLTERQIHFLEISLENEEKLPHYL
                     QPAYFTKALIYTLKYTAIRQSQLLQLKISDIDMTSKIINIRAETNKNHEHHEIPISDK
                     LYPYLKRLILESRARSTSSDEQLFNINRFSLVTTSRNKKMTEGQLVHFFRHISEFIGF
                     TVTSHRFRHTTATEVLKQSGDIYAVKQLLGHKDLTVTLTYIHNDPEMLRKHVNSL"
     gene            complement(921..1892)
                     /locus_tag="PXB27_002221"
     CDS             complement(921..1892)
                     /locus_tag="PXB27_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element ISApl1 family transposase"
                     /protein_id="HDL5346965.1"
                     /translation="MSTSYRHLTINEREKIMILLAQGKKQAEIAKALGRSSSTISREL
                     KRHALESYSATNAQNSYLKHRQNSKAQRKLEQPEYFNLVQEKFLTENWSPEQISARLK
                     LEKSELSISYSTIYRGIYSGLFDIGERKASRKLRHKGKTRHTKNHHEKRGKIQISNHL
                     NDRPISAQNRSRFGHWKADTVLGKAGGACLLTLTERKSRFELVKKIPAKKAEAVQKAM
                     IELLDSHILRSITPDRGKEFAQHRLVTEALGVEFYFPEPHQPWTRGTNENTNGLLREY
                     FPKHQDINQWSEVDIQQVVNKLNLRPRKCLGWKTPYEVYFKKTLHLV"
     gene            complement(2073..2837)
                     /locus_tag="PXB27_002222"
     CDS             complement(2073..2837)
                     /locus_tag="PXB27_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL5346966.1"
                     /translation="MLIEWRNQIVGDKIKAVTWNNYVRHLKSLYNFGLSQGVLNINQN
                     PFNQLFIRESKRRKKTYSTEQLHKIDHILNNNILLPDFLKPSWFILAIIMTLRCTGIR
                     RGQLVKLKIGDVNLSNRTIYISPEINKNHDYHIVPISDSLYLHIERLITELKIRKQTD
                     NCQLFNLNLFSRCSRRRGQPMSQNQLTHIFRHISDLVGFTSSPHRFRHTVATQLMKKP
                     ENVYVVQKLLGHKDISVTLGYIEHDVEMLRETVNLL"
     gene            complement(2993..4972)
                     /locus_tag="PXB27_002223"
     CDS             complement(2993..4972)
                     /locus_tag="PXB27_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HDL5346967.1"
                     /translation="MQSIFKKIFGKHTSQSAVKSDTVFSIEQDDDGWLYPQNATQLLN
                     TELRQKYLGLLWQQVSMNRQMFETFYQQPIERYAEMVQLLPASESHHHSHIGGMLDHG
                     LEVIAFSAKARQSYILPPNVAPEEQSKQRDAWTAAAIYLALVHDIGKVVVDIEITLKD
                     GSRWFAWNGEPPEPYKFKYIKGRDYHLHLVIGSFFANQIIPKSAFDWLAQYPEVISSL
                     MYAMSGHNDKAGLLSEIVQKADQYSVTLALGGDVNKLVQRPVNSLSKQLVLALRYLLE
                     QKIKLNTPKGPADGWFTEEGLWLVSKTAADNIRTYLLGQGISVPKDNPKLFDEMQSLG
                     IVEESPSGGAIWSCRIQADSGWTPANVFTLLRIKPEVAWENINDRPNYFSGKVKIVEQ
                     IMEDSNMENHPPLVDESTKLVTLENVQPETIITSKEVEISQVEASSDNAPFSEIIIND
                     ENDLTSFVLDMFAPTESSEPSLADISIGKGQEIATMPSKQEVQDKPTTKKLNVTQSAV
                     ETPETLTLSELQSQNKENSITGDNFVNWLKSGIATNKFAINKPTAKLHIVEDHLFLVT
                     PVIFEMYLQESGLPYDKEMINSLQYDFQGLGIHLPKNTTINGKPDTINFWKCDVIGPR
                     KTSSLTGYLIKNTHLFFGDKTLLNNHCLTLQKEET"
BASE COUNT         1516 a          973 c          941 g         1710 t
ORIGIN      
        1 gagtacccta aaaatttatt taaaggcctt atggtaagac ctgataagcc aaagaaaaaa
       61 ctactaacag aaaggcaaat ccatttttta gaaatctctt tggaaaatga ggagaaatta
      121 cctcattatt tacaacctgc ttattttact aaagctttaa tctacacttt aaaatataca
      181 gctatcagac aatcccagct attacagcta aaaatttctg atatcgatat gacaagcaaa
      241 attataaata taagagctga aaccaataag aatcatgaac accatgaaat tccaatttca
      301 gataaattat atccatacct aaaaagattg atattagaat caagagctag aagcactagt
      361 tcagatgagc agttatttaa cattaatcgc ttttcattag ttactacatc aagaaataag
      421 aaaatgacag aaggacaact agttcatttt tttcgccata tttctgaatt tataggattt
      481 acagtaactt cacatcgatt ccgacataca actgcaacag aagtcctaaa gcaatcaggg
      541 gatatctatg cagtgaagca attacttgga cataaagatc tcactgtaac tctaacatat
      601 attcacaatg atcctgaaat gctaagaaaa catgtcaatt cgttgtagaa atgatgctac
      661 atagataaaa taagggactg acgtagatta gtaagtatgc tagtctacgt cagccccgat
      721 tttttaatta atccagagat aactttgtca tcgggtgcaa tttatttaaa cctttctttt
      781 gataaagctc ttagaactaa aaccagagtc tttgctctca cattgataaa tgacgatcac
      841 ttatttattc cacaatcttt actgaatctt aggaccatga ataattttcc taagggtgac
      901 aaaattattg tcttgaattg tcaaaccaag tgcaacgttt ttttgaagta aacttcataa
      961 ggtgttttcc aacccaaaca tttacgcggt cgtaaattca gtttattgac cacctgttga
     1021 atatcaactt cgctccactg attgatgtct tggtgcttcg gaaagtattc acgaagtaac
     1081 ccatttgtat tttcattcgt tccccgtgtc cacggttgat gcggctcggg gaagtaaaat
     1141 tctacaccca gtgcttctgt taccaaacga tgttgggcaa attctttacc acggtctggc
     1201 gtaattgacc gcaatatatg tgaatccagc aattcaatca tggctttttg gactgcttcg
     1261 gcttttttgg caggaatttt cttcaccaac tcaaaacgac ttttgcgttc cgtcagcgtc
     1321 agcaaacaag ctccacccgc tttacccagt acggtatcgg ctttccaatg tccaaagcga
     1381 ctgcgatttt gcgccgaaat gggacggtcg ttcaaatggt tggatatctg aattttgcca
     1441 cgtttttcat gatgattttt tgtatgccgt gttttgcctt tgtggcgcag tttgcgactg
     1501 gctttgcgtt cgcctatatc aaacaaccct gaataaatac cacgataaat ggttgaataa
     1561 ctaatggata attcagattt ttccaatttt aatcgtgcgc tgatttgttc gggcgaccag
     1621 ttttctgtca gaaacttttc ttgcaccaaa ttgaaatatt caggctgctc taatttgcgc
     1681 tgtgctttgc tattttgacg atgcttcaaa tagctgtttt gtgcgttcgt tgcactgtag
     1741 ctttctagag cgtgtcgttt cagctcgcga gaaatggtgc tggagctacg tcccagtgct
     1801 ttggcaattt ctgcttgttt tttgccctgt gcgagtaaaa tcattatctt ttctcgctcg
     1861 tttattgtaa gatgtcggta ggaagtactc atcatttgga agtggttttt gtgtggaaac
     1921 aaaattatat tctagttgat gagtacttct ttttttgttg cacttggatt gtaaattcag
     1981 cgtataaaat cagttcattt agaagataaa acagaaatta tttatttata gaaaaaaagt
     2041 gggagaaatt atattctccc atttttcttt tatcagagta aattcactgt ctctctcaac
     2101 atttccacat cgtgctcaat ataacctagt gttacgctga tatccttatg ccctagaagt
     2161 ttctgaacca cataaacatt ttcgggcttc ttcattaatt gagttgcaac cgtatgtcga
     2221 aatctatgtg gtgatgaagt aaaaccgacc aagtcagaaa tatgccgaaa tatatgcgta
     2281 agctgatttt ggctcattgg ctgtcctctt cttcgagagc atcgagaaaa taaattgaga
     2341 ttgaacaatt ggcaattatc tgtttgtttt ctgattttta actctgtaat aagcctttct
     2401 atatgaagat aaagtgaatc tgaaatcggt acgatatggt aatcatgatt tttattgatt
     2461 tctggcgaaa tataaatcgt cctgttggaa agatttacat caccaatttt cagtttcact
     2521 aattgtcctc gccgaatgcc tgtacatctc aatgtcataa taatcgcaag aataaaccaa
     2581 cttggtttta gaaaatcagg taatagaatg ttattattga gtatgtggtc tattttatgt
     2641 aattgttcgg ttgaataagt tttctttctt ctttttgatt cacgaataaa taactgatta
     2701 aatggatttt gatttatatt gagtacccct tgcgatagac caaagttata taacgatttt
     2761 aaatgtctga cataattatt ccatgttaca gctttgattt tatcgccaac aatttgatta
     2821 cgccattcaa ttagcatatc agtggtaatc tcatttgcat ttaactttgg gtaacgtctt
     2881 ttgataattc ggattacatt attatagctc cgcttagtat cagggcgaag atagtaacga
     2941 tagaaaaaaa aatcggtcta gtagcgaatc aaaagagata gatgtcgtca ttttaagttt
     3001 cctctttctg taaagttaaa cagtgattat ttaatagtgt cttatcccca aagaagagat
     3061 gtgtattttt tatcaaatat ccagttaaag aggaagtctt tctcggacca attacatcac
     3121 atttccaaaa attgatggta tctggcttac cgtttattgt cgtattctta ggtaaatgga
     3181 ttcctagccc ttggaaatca tactgaagac tatttatcat ttccttatca taaggcagac
     3241 cactctcctg caaatacatt tcaaaaataa cgggggtaac taaaaacagg tggtcttcaa
     3301 caatatggag cttggcagta ggcttgttaa ttgcaaattt gttggtcgca atacctgatt
     3361 tgagccaatt tacaaagtta tctcctgtaa ttgagttctc cttattctgt gactgtagct
     3421 ctgatagtgt taatgtttcg ggcgtttcta ctgcactttg tgttacgttg agctttttag
     3481 tggtaggttt atcctgtact tcttgtttgg atggcatcgt tgcaatttcc tgtcccttac
     3541 caattgaaat atcagcaagg cttggctcag agctttccgt aggtgcaaac atatccagca
     3601 caaaagaggt taaatcattt tcatcattta tgatgatttc tgagaacggt gcattatctg
     3661 agcttgcctc tacctgagat atttcaactt ccttagaggt aataatagtc tctggttgaa
     3721 cattctctaa tgtaacaagt ttagtgcttt catcaactaa tggaggatga ttttccatat
     3781 tgctatcttc cattatctgc tcgactattt taactttccc ggaaaaataa tttgggcgat
     3841 cattaatatt ctcccaagcc acttcaggtt taattctcaa taatgtaaag acgtttgccg
     3901 gtgtccaacc tgaatcagct tgaatacggc aactccaaat cgccccacca gaaggagatt
     3961 cttctacgat accgagagat tgcatttcat caaataactt tggattatcc ttaggcacgg
     4021 aaatcccctg ccctagtaag taagttcgta tattatcagc ggcagttttg ctaacaagcc
     4081 acaatccttc ctcggtaaac caaccgtctg cagggccttt cggagtattt aacttgattt
     4141 tctgttccag taaataacgt agtgccaaga caagctgctt tgacagagaa ttgacaggac
     4201 gttgaaccag tttatttaca tcgccaccga gtgcgagggt aacactatac tgatcagctt
     4261 tttgcacgat ttcagataaa agcccagctt tatcattatg tccggacatt gcatacatca
     4321 gagaagaaat gacttctgga tactgtgcca gccaatcaaa tgcagatttg ggaataatct
     4381 gatttgcaaa gaagctgcct atcacaagat gaagatggta gtctcgacct ttgatgtatt
     4441 tgaatttata cggttcagga ggttctccat tccaagcaaa ccaacgtgag ccatctttga
     4501 gtgtgatttc aatatctact acaactttgc caatatcgtg aactaatgca agataaattg
     4561 ctgcggcagt ccaagcgtct cgctgctttg actgttcttc gggagccaca ttcggtggca
     4621 aaatataact ctggcgagct ttagctgaaa aagcaataac ttccaaaccg tgatcgagca
     4681 ttccaccaat atgagaatga tgatgtgatt ctgaagctgg caatagttga accatttcag
     4741 cgtaacgttc tattggttgc tgataaaatg tctcgaacat ttgacggttc attgatacct
     4801 gttgccaaag tagtccaaga tacttttgac gtaattccgt attcagtaat tgagtagcat
     4861 tttggggata tagccaacca tcgtcatctt gctcaataga gaaaacagtg tcagatttga
     4921 ccgcactttg agatgtgtgt ttaccaaaaa ttttcttgaa aattgattgc attgaaagag
     4981 accgaaaagt atcaattttg tgttaaatga aagccgttta gccctttgcc cttttgggca
     5041 agacctttcc ccttggaaag gttacctttc accctttagg ggttatcctt tgcccttaag
     5101 gactccttta tccttttgcc atttagggtg agcttggaat
//
LOCUS       DANLUQ010000159         5053 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.160, whole genome shotgun sequence.
ACCESSION   DANLUQ010000159 DANLUQ010000000
VERSION     DANLUQ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 5053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5053
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.160"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..633)
                     /locus_tag="PXB27_002224"
     CDS             complement(<1..633)
                     /locus_tag="PXB27_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HDL5346968.1"
                     /translation="MAEGLLLTPYFQTEVGIVFYKVPKDVSPAAFGERTLLQPAPTDL
                     ATVKSGKVANFSANSTACKELAVFAKSAKVRSTLNQRDKYSNDLPYIRFVKSIQTCQC
                     RDGKYCHTEKVLVENKNGAVQLCWHHDKMRMDGEIPNEKLEALADENWEKFIAQQIRL
                     TLRKTQSTAIEFADLVLFASIKGLLDELDSKALQQFLGYPPLNNSGVSKES"
     gene            complement(760..1353)
                     /locus_tag="PXB27_002225"
     CDS             complement(760..1353)
                     /locus_tag="PXB27_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine methyltransferase"
                     /protein_id="HDL5346969.1"
                     /translation="MTDFDKNTYQTPNYVRNWLNHRYAWFHIDGCSNGQNTLYTYWIG
                     RAADGLDDDKLSCQIADDFLADDLFDVLLDKVSDWGELLRIFVNPPYSDPLPFVQRAA
                     ELKKAGHLVVMLLPADKTTEWYTIIQQHANEVIDIIGYHDEKGTWRTGRIQFINPVTG
                     KPAQGNNKGSMIVVFDPFIEGIVTRQMPLDKIKEWGK"
     gene            complement(1364..2410)
                     /locus_tag="PXB27_002226"
     CDS             complement(1364..2410)
                     /locus_tag="PXB27_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HDL5346970.1"
                     /translation="MRFTSTINNVRLVEWEINITQGALVDLINQASSWAKAVVIDGIT
                     YYWMSFSKVCEELPAVFSKEDTVYRQYKVLKEKGIIDHFKMDGKDYVRLTEKGCEWNK
                     FEPIRESEKNPSIGNNSEQTRKNFRESSEKNPTDNNTNYKNNNDHTTPLNPPAGEPAP
                     AEVVLNYLNSALVTLAVQLGERKPVGYSLKPWAKNITARIRESSVADCCQVVDYLVAK
                     WGRDEKMREYLCPKTIFRQSNFADYFPKSTAWANNGKPVCVNGKWVTPAELEKRLIMP
                     TVDEVRALFQKSLSGNPFKALDFTNKRNLVMYHATINTRNKRPLERDLPMIISQEIKN
                     AVERIDRLRVPTFS"
     gene            complement(2407..3090)
                     /locus_tag="PXB27_002227"
     CDS             complement(2407..3090)
                     /locus_tag="PXB27_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein/antirepressor Ant"
                     /protein_id="HDL5346971.1"
                     /translation="MNQLLNISAPKASITMSSREIAELCEKEHRHVTRDIEAMFSQLD
                     IPAEGYAHFWTHPQNGQTYREFRLPYDLTLTLISGYSVILRKKIIDRWQELESRQGIP
                     QSFSQALRLAAEQQEVIEQQNHQLAIQAPKVAFVEHYVEVGTTKSLRETAKILNFPEK
                     RLIECLERYRVLYRQSGNLLPYQDKQAKQLFAVKTGTAEHGHNFTQTRVTAKGIEWIA
                     QRYASELGQ"
     gene            complement(3148..3336)
                     /locus_tag="PXB27_002228"
     CDS             complement(3148..3336)
                     /locus_tag="PXB27_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDL5346972.1"
                     /translation="MKKDDVLEHFGTLEKVAATLGISVSAVSQWGEIIPEKNAYRLQE
                     ITAGKLKVKHSLYRNRSK"
     gene            3461..4117
                     /locus_tag="PXB27_002229"
     CDS             3461..4117
                     /locus_tag="PXB27_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5346973.1"
                     /translation="MNTQTLGDRIKSRRKELKLTQKDLATALKGVSHVAVSQWESSTT
                     KPNSENILDLSTVLQCDISWLLRGNGESNVLPASLGMTKVPLISYIQAGQWTGIDDFR
                     ETCGDYEYILTDLEVSEDAFALEIKGDSMEPDFIEGDRVIIDPDVQPHAGEFVAAING
                     DYEATFKKYRPLEDLDEYGRQHFELIALNPDYHKLSTLKQEIRIIGTMVEHRIYRRKR
                     "
     gene            4129..4503
                     /locus_tag="PXB27_002230"
     CDS             4129..4503
                     /locus_tag="PXB27_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346974.1"
                     /translation="MTEFKDVKQAIVEAQLASTPKDKVVNDIMKGSAELLSANDIINR
                     LGIGADEFQELVNLPNHYLRINGGLGAAVYNTRRMFDTHVELVNQELEKNISFPKPDL
                     YILGKARWKKETFKKWLEEQCR"
     gene            4655..>5053
                     /locus_tag="PXB27_002231"
     CDS             4655..>5053
                     /locus_tag="PXB27_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HDL5346975.1"
                     /translation="MLSKQVLSISESRLSTYLFCFFRNDPTKQKEAIAIYTALQHRTG
                     IYFSLIQEIEVALRNEVSQLLRFAAPNHNLYEFFHFLANDSNAPLNAESKRQLQKAIS
                     ECGRNYNENDIISHISFGFWVNLFNYDIKRN"
BASE COUNT         1466 a         1033 c          975 g         1579 t
ORIGIN      
        1 actttctttg ctcacaccgc tattattcag tggtggatag ccgagaaact gctgtaatgc
       61 cttgctgtct aattcatcaa gcaaaccttt gatactcgca aacaacacta agtccgcaaa
      121 ttcaatagcg gtagattgcg ttttgcgtaa ggttaagcgg atttgttggg cgataaattt
      181 ttcccagttt tcatccgcta gtgcttctaa tttttcattc gggatttcac cgtccattcg
      241 cattttgtcg tgatgccaac aaagctgtac cgcaccgttt ttattctcta ccagcacttt
      301 ttccgtgtgg caatatttgc catctcggca ttggcacgtc tgaattgact ttacaaaccg
      361 gatataaggc aaatcattac tatatttgtc acgctgattt agcgttgaac gaacttttgc
      421 cgatttagca aacaccgcca gctctttaca agcggtcgaa tttgccgaaa aatttgcaac
      481 cttgcctgat ttaaccgtcg ctaaatcggt cggtgcaggt tgcaacaaag ttcgctcacc
      541 gaaagcagca ggagaaacat ctttaggcac tttgtaaaac acaataccca cttcggtttg
      601 aaagtaaggg gtaagtagca aaccttccgc cattattgcc cccatttctc ggtttcatca
      661 taggctttca cgaatagcat aaatacgcca atacagatta aaaaaatcag tacgccaatt
      721 actgcaccaa taccaataaa tagttcccaa taatccattt tatttacccc attccttgat
      781 tttatctagt ggcatctgac gagttacaat gccctcaata aatgggtcaa acacaacaat
      841 catcgagcct ttattattac cttgtgccgg tttacctgtt accgggttga taaattgaat
      901 ccgccctgtc cgccaagtgc ctttttcatc gtgatagcca ataatatcaa ttacctcatt
      961 agcgtgttgt tgaatgatgg tgtaccattc tgttgtttta tccgctggta ataacatcac
     1021 aaccaaatga cctgcttttt tcaattctgc cgctcgttga acaaaaggca gtggatcaga
     1081 atatggcgga ttaacgaaaa tacgcagtaa ttctccccag tctgaaactt tatctagtag
     1141 cacatcaaat aaatcatcgg ctaaaaaatc atcggcaatt tggcaactta atttatcatc
     1201 atctaaaccg tctgccgctc taccaatcca ataggtgtac aacgtatttt gtccgttaga
     1261 gcaaccgtca atatgaaacc aagcataacg atgatttagc caattacgca cataattagg
     1321 tgtttggtaa gtgtttttat caaaatctgt cattgtctaa tccttacgaa aatgttggta
     1381 ctctcaaacg gtcgatacgc tcaaccgcat ttttaatttc ttggctgata atcatcggca
     1441 aatcacgttc aagtggtcgc ttgtttctgg tgttgattgt ggcgtggtac atcactaaat
     1501 ttcgtttgtt ggtaaagtcc aatgccttaa acggattgcc actgagtgat ttctggaaca
     1561 aggctcgtac ttcgtccacc gttggcataa tcaagcgttt ttccagctcc gccggtgtaa
     1621 cccacttacc atttacgcaa acaggctttc cgttattagc ccaagcggtc gattttggaa
     1681 aataatctgc aaaattcgat tggcggaaaa tcgttttcgg gcagaggtat tcacgcattt
     1741 tttcatctcg tccccattta gcgacaaggt aatccaccac ttggcaacag tccgccaccg
     1801 aactttcacg aatgcgagca gtaatatttt ttgcccacgg cttgagcgaa taccccacag
     1861 gcttacgctc accaagctgt accgccaacg ttaccaaagc cgaattgaga taattcaaca
     1921 caacttcagc cggggcaggt tcccccgctg ggggattaag gggggtagta tgatcattat
     1981 tatttttata attagtatta ttatctgtcg gatttttttc cgagctttct cggaaatttt
     2041 tccgagtttg ttcggagtta tttccgatac tcggattttt ttccgactca cggatcggct
     2101 cgaatttatt ccattcacaa cctttttcgg ttaaacggac ataatcttta ccgtccattt
     2161 tgaagtggtc gataatgcct ttttctttca acaccttata ttggcggtaa actgtatctt
     2221 ctttgctaaa aaccgcaggt aactcttcgc aaaccttgct aaatgacatc cagtaatagg
     2281 ttatgccgtc aataaccacc gctttcgccc aactggaggc ttgattgatt aaatcgacca
     2341 atgccccttg tgtaatattg atttcccatt ccactaagcg gacattgttt atggttgatg
     2401 taaatctcat tgtcctaact ccgatgcgta acgttgagca atccattcaa tccctttggc
     2461 ggttactctc gtttgggtaa aattatgacc gtgttctgcc gtgccggttt ttactgcaaa
     2521 aagctgttta gcttgtttat cttgataagg gagtaggttt cctgattggc gataaagaac
     2581 tcgatatcgt tctaaacatt caatcaaccg cttttctgga aaattgagta tcttggcggt
     2641 ttctcgcaat gatttagtcg ttcccacctc aacataatgc tccacaaatg cgactttcgg
     2701 ggcttgaatg gctaattgat gattctgctg ctcaatcact tcttgctgtt ctgccgccaa
     2761 ccgtagagct tgagagaaag attgtggaat accttgtctg ctttctagct cttgccaacg
     2821 gtcgataatt ttcttgcgaa gtatcacgct atagccggaa attagggtaa gggttaaatc
     2881 gtaaggcaat cgaaattccc ggtatgtttg cccattttga gggtgtgtcc aaaaatgggc
     2941 atacccctcg gcaggaatat ctaattgact aaacatcgct tctatatcac gagtaacgtg
     3001 tcgatgttct ttttcgcaca actcagcaat ctcacgacta ctcatcgtaa tgcttgcttt
     3061 tggtgccgaa atgtttaata attgattcat cgaaaatacc tttcgtgttt aattatgtat
     3121 gaaagccacc gttacagcgg tggtttttta tttgcttcta tttctataaa gtgagtgttt
     3181 gacctttaac ttgccggcag tgatctcttg taatctgtac gcatttttct caggaataat
     3241 ctcgccccat tgagaaacag cagatacact aatccctaaa gttgccgcca ctttttctag
     3301 agttccgaaa tgttcgagca catcatcttt tttcatattt tcttaattcc tttcttgaat
     3361 tttatttaag acctcttaaa tatacaatta aaggaaactt aagtcaataa atgttaagat
     3421 aacttaacag caactttatt tttaccttta aggggatact atgaatactc agaccttggg
     3481 cgatagaatt aaaagccgta gaaaagaatt aaaattgaca cagaaagatc ttgctactgc
     3541 gttaaaaggg gtctcacacg ttgcagtatc tcaatgggaa agtagtacga ctaagccaaa
     3601 ttcagagaat attttggatt tatctacagt attgcaatgc gatattagtt ggctcttgcg
     3661 tggtaatgga gagtctaatg ttctacctgc tagcctcggt atgactaaag tcccattaat
     3721 tagctatatt caagccggac aatggacggg aattgacgat tttcgtgaaa cctgtgggga
     3781 ttatgaatat atcctaactg atcttgaggt gtctgaagat gcttttgccc tagaaatcaa
     3841 aggggactcg atggagcctg attttattga aggagaccga gtaattattg atcctgatgt
     3901 acaacctcac gccggtgagt ttgtggcagc aattaatggc gattatgaag ccactttcaa
     3961 aaaataccgt ccgttagaag acttagacga atacggaaga caacattttg agcttatcgc
     4021 tcttaatccg gattaccata aattatcaac gctaaagcaa gaaatcagaa ttatcggcac
     4081 aatggtagag catcgtattt atcgtagaaa gagataggaa ttgataatat gacagaattt
     4141 aaagatgtaa aacaggcgat tgtagaagct caattagctt cgacacctaa agataaagtc
     4201 gttaatgaca taatgaaagg aagtgcggaa ctgctttcag caaatgatat tatcaatagg
     4261 ttaggtattg gggcagatga gtttcaagag ttggttaatt tacctaacca ttatttgcgt
     4321 ataaatggag ggttaggtgc tgctgtgtat aatacaagaa gaatgtttga tacccatgtt
     4381 gagttagtca atcaagagtt agaaaagaat atttcatttc ctaaacctga tttatatatt
     4441 ttggggaaag ctcgttggaa aaaagaaaca tttaagaaat ggttggaaga acagtgtaga
     4501 taaagaaaag gtaggttaaa aaacctacct tttctgaacc gcaaataagc gattctcaca
     4561 ctttacaaag caatccagct cctacgtctg gggcttcggg tccgttgcca gtgcaagtat
     4621 tattacatta aactaaactt gagtaaagga aaatatgttg tccaaacaag tgctaagtat
     4681 ttcagaaagt cggctgagta catatttatt ttgctttttc cgaaatgatc ccacaaaaca
     4741 gaaagaagct attgcaattt ataccgcctt gcaacatcga acaggaattt atttttcatt
     4801 gattcaagaa atagaggtgg cactaagaaa tgaagtcagc cagttgttac gctttgctgc
     4861 acctaatcat aatttatacg aattctttca ttttctagcg aacgatagta atgctcctct
     4921 taatgcagaa tctaaacgcc agctacaaaa agccattagt gaatgcggac gtaactataa
     4981 tgaaaatgac ataatttctc atatttcttt tggattttgg gtaaatctgt ttaattatga
     5041 tataaagcgt aat
//
LOCUS       DANLUQ010000160         4982 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.161, whole genome shotgun sequence.
ACCESSION   DANLUQ010000160 DANLUQ010000000
VERSION     DANLUQ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4982
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.161"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..1350
                     /locus_tag="PXB27_002232"
     CDS             <1..1350
                     /locus_tag="PXB27_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDL5346976.1"
                     /translation="NSTSKEMNATSVSSIPKKESVTNVPTLDKKETEQNLTQLQKDFS
                     AHQLENQKAKQSMINAQSELRRLNSQLNVLQKYVNSRRLGYYTQQAVLEQISIIQNKI
                     KQTQTIFNDANATVKLTDQKLEEAKLALGSVNDLVLIKASAPAMQATNQDTSMMNIIQ
                     ADWISQYANTALSELSAQANSALQISNSLDRQLFKQSDKFNVWSSVEHQKTEHKSDLY
                     RPYKQQTNLTQLGIQMPIDNGLMFGVALSKNHANAEFNEGVNGKSNLLMASLYGKWQS
                     QQGTFISLDGSYGKAKNQLYLFGENHFTRRISSIGANIGHQFDLAGVQIQPTIGARYY
                     HFSGQDYTLGGAKISSPNTHFMTYQAGLKASKTFSLDDWKVEPSITTHYVDASNKRLA
                     VNVNNNNINQRFGRYLKTEAGIRFEHNQWQFDLHAGFINGNEIKKQHFAGVKLGYSW"
     gene            complement(1405..2499)
                     /gene="mobA"
                     /locus_tag="PXB27_002233"
     CDS             complement(1405..2499)
                     /gene="mobA"
                     /locus_tag="PXB27_002233"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HDL5346977.1"
                     /translation="MSKTIEERISAVILAGGLARRMNGVEKGLQLFEGKPLISHILAR
                     LSPQVSDIWLNVNRSIEQYQQLYPAFSYYQDSLPDFQGPLSGMLAGFEQIESDYLLFV
                     PCDTPFMPELLLQKLKTALRINNVQIAYAHDGERPHPTFALIHRSVQEDLKAYLGSNQ
                     QQLLAFFQSQKSVAVDFSEQKSAFTNVNTLEDLSRVSQFPVKNLAITGYSGTGKTTLL
                     EKLIPKLTACGIRVGLIKHSHHNVDVDKKGKDSYRLREAGANPTMIVCDERWALMTET
                     KQAVEFSQLIAKFNPQEVDLVFVEGFKHETLPKIQLHRKGIEKPLPELDQWTIATATD
                     YPLERENWLDINNIGGIADFIKNWLENKAS"
     gene            2586..2855
                     /locus_tag="PXB27_002234"
     CDS             2586..2855
                     /locus_tag="PXB27_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1040 family protein"
                     /protein_id="HDL5346978.1"
                     /translation="MKCERLEELLELLGEHWRKNPDLHLIDILQQLSVEVGEPDNFKA
                     LSDEVLIYQLKMRNAGKFEPIPGIKKDYEDDFKTALLRARGILND"
     gene            2919..3557
                     /locus_tag="PXB27_002235"
     CDS             2919..3557
                     /locus_tag="PXB27_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDL5346979.1"
                     /translation="MKKLALKTAFVALSALFTFNSTAIAADPTAGKEYIEVRKAPSAQ
                     KEVVEFFSFYCPHCYDFELSYKIPSQIKEKLPSDSKLVQYHVNFLGRQSEDLTRAWAL
                     AMALGAEDKVKTALFEGAQKDAFKSMDDIRSVFLANGITAEQFDSGINSFAVNGLVNK
                     QVQLAEDFQIRGVPAFFVNGQYQLNLEGFADSSSTNDFIKRYVDAVVFLSKK"
     gene            3641..4417
                     /locus_tag="PXB27_002236"
     CDS             3641..4417
                     /locus_tag="PXB27_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDL5346980.1"
                     /translation="MTKLSILNLVPIREGQTAKQAVDSMVKLAKHAESIGIERYWIAE
                     HHNMKNLASSATQLLIQHALSHTEKLRVGSGGVMLPNHSPYIVAEQYGTLETLYPNRV
                     ELGLGRAPGTDMRTATALRRNAQSLPFPDEISELKGYFEGTNSVSAYPAQGLNVPFYI
                     LGSSPESAYLAAERGLPYAFASHFAPRFMDDAVRIYRTHFKPSEVLDKPYVILGVNAI
                     AAETDQEAEQLATTQTQFFLNVVTNAQNYLQPPVESDDKV"
     gene            4427..4681
                     /locus_tag="PXB27_002237"
     CDS             4427..4681
                     /locus_tag="PXB27_002237"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase"
                     /protein_id="HDL5346981.1"
                     /translation="MKAEIVPHFGPVSFQQNALYNQEKPVVRQMTACSLIGSRESIEQ
                     QLKSLKARIEIDEIMAVSYIFDEQKQHQSYTWLKEIADKV"
     gene            4683..4976
                     /locus_tag="PXB27_002238"
     CDS             4683..4976
                     /locus_tag="PXB27_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HDL5346982.1"
                     /translation="MSKTMQQKAADAHNMCKLKGDSMLDNSDRQIAFEAVYDSEEIAV
                     QAVEFFTQKAKSVETEPCDIQSEITPIIDGFLMKMWITFSCQAEVILFQMAVR"
BASE COUNT         1616 a         1010 c          958 g         1398 t
ORIGIN      
        1 aattcaacct ctaaagagat gaatgctaca tctgtatcct ctattccaaa aaaggaatct
       61 gttactaatg ttcctacttt agataagaaa gaaactgaac aaaatcttac tcaactacaa
      121 aaagattttt cagcacacca attagaaaat caaaaagcaa aacaatctat gataaatgct
      181 caatctgagc taagacgact caattcacaa ctgaatgtat tgcaaaaata tgtgaattct
      241 cgtcgcttag gttactatac tcagcaggca gttttagaac aaattagcat tattcaaaat
      301 aaaattaaac aaacacaaac aatatttaat gacgctaatg caactgtaaa actcacagat
      361 caaaagctag aagaagccaa attagctcta ggctctgtaa acgatcttgt attaataaaa
      421 gcctctgctc cagcaatgca agcaactaat caagatacga gtatgatgaa tattattcaa
      481 gcagattgga taagccaata cgctaacaca gcactttctg aactctcggc acaggctaat
      541 tctgctctgc aaatcagtaa tagcttagat cgccaactct tcaaacaaag cgataaattc
      601 aacgtatgga gcagcgtcga acatcagaaa accgagcata aatcagattt ataccgcccg
      661 tataaacaac aaaccaacct gacccaactg ggcatacaaa tgccgataga taacggttta
      721 atgtttggag ttgcattatc taaaaaccac gctaacgcgg aatttaacga gggtgtaaac
      781 ggtaaatcga atctactaat ggcaagccta tatggtaagt ggcaatctca acaaggcact
      841 tttatcagcc ttgatggcag ctacggtaaa gcaaaaaacc aactctacct atttggtgaa
      901 aaccacttta cccgccgaat ttcctctatt ggtgctaaca ttggacatca atttgacctc
      961 gcaggagttc aaattcagcc aacaatagga gcaagatact accatttcag cggccaagac
     1021 tatacactag gaggagcgaa aatcagctca ccaaataccc actttatgac atatcaagcg
     1081 ggtctaaaag ctagtaaaac cttttcattg gatgactgga aagttgaacc aagcattaca
     1141 acccactatg tggatgcaag taacaaacgc ttagcagtaa acgtcaataa taacaatatc
     1201 aaccaacgtt tcggtcgcta tctgaaaacc gaagctggta tccgatttga acataaccaa
     1261 tggcaattcg acttacacgc tggtttcatt aacggcaacg aaatcaaaaa acaacatttt
     1321 gctggtgtta agctaggata tagctggtaa gaaaaagccc ctagaactat ttctaggggc
     1381 tttttctact cttaaaaaga gtatctaact tgccttattt tctaaccaat tcttaataaa
     1441 atctgctatt cccccaatat tattaatatc caaccaattt tctctctcaa gcgggtaatc
     1501 cgttgcggtg gcaatcgtcc attggtctag ctctggcaat ggtttttcaa tccctttgcg
     1561 gtggagctga atttttggca aggtttcgtg tttaaagcct tctacgaaaa cgagatcaac
     1621 ctcttgcggg ttgaattttg caattaattg agaaaattcg accgcttgtt tggtttctgt
     1681 cattaatgcc caacgttcat cgcacacaat cattgtcggg tttgctcctg cttcacgtag
     1741 gcggtagctg tctttgcctt ttttatctac atcaacgtta tggtgggaat gtttaatcag
     1801 cccaacccga ataccgcaag cggtcaattt tgggattaat ttttccaata aggtggtttt
     1861 acctgtgccg ctgtagcctg taattgcaag gttttttaca gggaattggc ttactcggct
     1921 taaatcttcc aatgtattca cgttggtgaa tgccgatttt tgttcggaaa aatcaactgc
     1981 cacgcttttt tgtgattgga agaacgcaag caattgctgt tggtttgagc ctaaataggc
     2041 tttcaaatct tcttgtaccg aacgatgaat caacgcaaaa gtgggatgtg gacgctcacc
     2101 atcatgggca tatgcaattt gtacgttatt tatccgcaaa gcagttttta atttttgcaa
     2161 taacagctca ggcataaagg gcgtatcgca aggcacaaaa agcaaatagt cactttcaat
     2221 ttgctcaaat cctgccagca ttccactgag tggaccttga aaatcaggca agctatcttg
     2281 atagtaagaa aaagcaggat aaagctgctg atattgctca atagaacgat tgacattaag
     2341 ccaaatgtca ctaacttgtg gtgaaagtcg ggctaaaatg tgtgaaataa gcggttttcc
     2401 ttcaaaaagt tgtaaccctt tttctacccc attcattcga cgagctaaac cgcccgctaa
     2461 aatcacggct gaaatgcgtt cttctattgt tttactcaca gctttatccc ttaaaatttt
     2521 gcaaaaataa gagtaaaagt atattatttc gtttaatttc caacaagttt tttaaggggt
     2581 aaaagatgaa atgcgaacgt ttagaagagt tattagagtt acttggcgaa cattggcgta
     2641 aaaatcctga cttacacctc attgatattt tgcagcagct ttcagttgaa gtgggcgagc
     2701 ctgataattt caaagcgtta agcgatgaag tgttaatcta tcagcttaaa atgcgaaatg
     2761 caggcaaatt tgagcctatt cccggcataa aaaaagatta tgaagatgat tttaaaacgg
     2821 ctttattgcg agctcgtgga attttaaacg attaagattg tcttatccgt tagctagttt
     2881 agcatttatt atttatcaac atttttgaag gaatcattat gaaaaaattg gcattaaaaa
     2941 cggcatttgt tgcactttct gcccttttta catttaactc aacggcaatc gctgctgatc
     3001 caaccgcagg caaagaatat atcgaagtgc gtaaagcacc ttctgcacaa aaagaagtgg
     3061 tggaattttt ctcattctac tgcccacact gttatgattt tgaattaagc tacaaaatcc
     3121 caagccaaat caaagaaaaa ttaccaagcg atagcaaatt agtgcaatat cacgttaatt
     3181 tcttaggtcg ccaatctgaa gatttaactc gtgcttgggc attagcaatg gcattaggtg
     3241 cggaagataa agtaaaaacc gcattatttg aaggtgctca aaaagacgca ttcaaatcaa
     3301 tggacgatat tcgctctgta ttcttagcaa acggcattac agctgaacag tttgatagcg
     3361 gtattaatag ctttgcggta aatggcttag tgaataaaca agttcaatta gcggaagact
     3421 tccaaattcg tggtgtgcct gcattcttcg taaatggtca ataccaactc aacttagaag
     3481 gctttgcaga ttcttcatca acgaatgatt tcatcaaacg ttatgttgat gcggttgtgt
     3541 tcttaagcaa gaaataaccc cttaaaatag ttagggtttt tacaatgctg agtaggtaaa
     3601 tcctattcag cattttttat atgcaaactc ggagaacaaa atgacaaagt tatctatcct
     3661 aaaccttgta cctattcgtg aagggcaaac tgccaaacaa gcggtcgatt ctatggtgaa
     3721 attagcaaaa cacgcggaaa gtatcggcat tgaacgctat tggattgcag agcaccacaa
     3781 tatgaaaaac cttgccagct cggcaacaca gctcctcatt cagcatgccc tttctcacac
     3841 tgaaaaatta agagtgggat caggtggcgt gatgttgcct aaccatagtc cttatattgt
     3901 ggcagagcaa tacggtacgc ttgaaacact ctaccctaac cgtgtagaac ttgggcttgg
     3961 gcgagcacca ggcaccgata tgcgaaccgc taccgcttta cgccgtaatg cacaaagtct
     4021 gccatttcct gatgaaatca gcgaactaaa aggttatttt gaaggcacaa attctgtgtc
     4081 tgcctaccct gcacaggggt tgaatgtgcc gttttatatt ttaggctcaa gccctgaaag
     4141 tgcctattta gcagcagaac gtggcttgcc ttatgccttt gcttcacatt tcgctcctcg
     4201 ttttatggac gatgcggtgc gaatttatcg cacacacttt aagccgtctg aagtgttaga
     4261 taaaccctat gtgattttag gtgtgaatgc gattgcggca gaaaccgacc aagaggcaga
     4321 acagcttgct accactcaga ctcagttttt cttaaatgtt gttaccaatg cccaaaacta
     4381 tttacaaccg cccgtcgaat ccgatgataa agtgtgaaaa aattttgtga aagcggaaat
     4441 cgtaccgcat ttcgggcctg tttcgttcca acaaaatgcg ttatacaacc aagagaaacc
     4501 cgttgttcgc caaatgactg cttgttcact tatcggtagc cgagagagca ttgaacaaca
     4561 gctcaagtca ttaaaagcac gcattgaaat tgatgaaatt atggcggtaa gctacatttt
     4621 cgatgagcaa aaacagcatc aatcctatac ttggttaaaa gagattgcag ataaggtgtg
     4681 atatgagcaa aacaatgcaa caaaaagccg ctgatgcaca taatatgtgt aagctaaaag
     4741 gcgactctat gttagataac tcagaccgcc aaattgcctt tgaagcagtg tatgatagtg
     4801 aagaaatagc tgtacaagcg gtcgaatttt tcacacaaaa agcaaaatcg gtagaaaccg
     4861 agccttgcga cattcaaagt gaaatcacac ccattataga tggctttctg atgaaaatgt
     4921 ggataacttt cagttgccaa gcagaagtaa tcttatttca aatggcggtg agatagatat
     4981 aa
//
LOCUS       DANLUQ010000161         4880 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.162, whole genome shotgun sequence.
ACCESSION   DANLUQ010000161 DANLUQ010000000
VERSION     DANLUQ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4880
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.162"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(87..653)
                     /locus_tag="PXB27_002239"
     CDS             complement(87..653)
                     /locus_tag="PXB27_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346983.1"
                     /translation="MEGLIMGLQLIPHFPELAISRRTYLHDMPMYCFVSKNWIFSAKD
                     RAKREALGNLLACGGEMMKSFIFKRSLFSEIWFEDILLEDEEKELIRILYNKPEPEYQ
                     VEEKIDWEAREARRKQADYDKWQSYDERNPFHYQIFPTDTIAYAQQQVEKFIEDVDNF
                     LEWFYDDEVKRYILERNEIYQKKFDLVN"
     gene            complement(1186..1512)
                     /locus_tag="PXB27_002240"
     CDS             complement(1186..1512)
                     /locus_tag="PXB27_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5346984.1"
                     /translation="MLHSKGKILPKITLNEIETKERFAIALRKEMHAQYWTGKMVANA
                     LDVSEKTVKDWVAGKRLPSSLDLLALMRISSCVRQLVWDISKSEECYQDAEMLKQICF
                     VLLSNL"
     gene            1560..3191
                     /locus_tag="PXB27_002241"
     CDS             1560..3191
                     /locus_tag="PXB27_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL5346985.1"
                     /translation="MVTRTKNQPKNQAVSYIRMSTEHQEFSPDLQRCFIENYAKENGL
                     EIIREYIDEGKSGLTAEHRPQFLSMIQLVQSGQADFDHILVYDVSRWGRFMNIDESGH
                     YELICTYQGIKVHYCAEPFKGNDIASQIYKTMKRLSVGDYCRELGVKVFNGQKNLIQH
                     GFRQGGVAGFGLRRRLIDCQGNPKFDLQRGDRKSLQTDRVILVAGPKEEQEIVRQIYH
                     DFVYQHKTEQQIADSLNAQGIVTDRNTAWTKGVVHQILINEKYVGHNVWNKRSASKTR
                     EYKGKNPVEEWVRAENVFEAIVPQALFNAAQTIIHQRSARLSDDEMLEQLKLLLKEKG
                     KLSGLIIDEAENCPSSSAYSSRFGSLLNTYTLINYEPERDYHYVEINRLLRQQHKNFV
                     QQTLDTIIELGGVVLSDDVTDLVHINNEFTASIVLSRCRLTPSGSKRWLIRFDTSLNP
                     DITIAVRINESATEILDYYLLPTTEKVNEKLRLAESNPAELEIYRHDNLDRFFIMVER
                     ILVKEFIYAKRHHSNSSYRQNSGTQSQNAQSESLC"
     gene            3106..3999
                     /locus_tag="PXB27_002242"
     CDS             3106..3999
                     /locus_tag="PXB27_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HDL5346986.1"
                     /translation="MQNDTIQILPIDKIRVLNPRTRNQKVFADLVENIATIGLKRPIT
                     VTPSVDSAEYYDLLCGQGRLEACQVLGELFIPCRVVNANSEESYLISLVENIARRQHS
                     YMELLSGIKILNDRGYKPVDIARKIGFSKSHVNSILHLINAGEERLINAVEQGILPIT
                     IAISISRSDDQEVQKQLAELYTNGTLKSTDIAKIRNLIHRRNLAGKKANTGISGSIRS
                     SIYNPKTVVNIYKEETERQKMMIKQADYDEKQLSIILSCLNKLFEDKYFQLVLKSEHL
                     DDMPKDLSQRMLKQREGNIYA"
     gene            3992..4855
                     /locus_tag="PXB27_002243"
     CDS             3992..4855
                     /locus_tag="PXB27_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HDL5346987.1"
                     /translation="MPKHITYAFHNQLISLPIDEIIPLKKMDKNFEKSTKFQSILASI
                     REIGIVEPVVVYPEENNQYILLDGHSRVHALKKLGVDKVLCMISTDDEGFTYNKQINR
                     LTAVQENKMLMKTIERGVSDELIARTLNINLKALRQKMNMLNGITREVVDILANKQVS
                     KEIFRILRKMKPERQIEVAQMMIASNKFTLTYANMMLLSSRKEELVESHKSKTTNDDL
                     TDLSIMQKELERLKENYKISEEKLADLKMGLVVAKGYVKKLLNNAAVVDLLENDQSEI
                     YLTLKEVCDIQ"
BASE COUNT         1614 a          932 c          940 g         1394 t
ORIGIN      
        1 agtagttacc ctaaatctac aaatatcaag tgtaaaaaaa tataaaagtc ggcaataaca
       61 ttattttata ataactaatg tgaattctag ttcactaaat cgaatttctt ttggtaaatc
      121 tcattccttt ctaatatata gcgttttacc tcatcatcgt aaaaccattc aagaaagtta
      181 tcaacatctt caatgaactt ttctacctgt tgctgtgcat atgctatcgt atcggtaggg
      241 aagatttgat aatgaaacgg attgcgttca tcataactct gccatttgtc ataatcggct
      301 tgtttacgcc gcgcttctct tgcctcccag tcgatttttt cttccacttg atattctggc
      361 tctggcttat tatacaaaat acggatgagt tccttttctt catcctcaag cagaatatct
      421 tcaaaccaga tttcagaaaa taggctgcgc ttgaaaatga aagacttcat catttcccct
      481 ccacacgcca ataagtttcc caatgcttcc cgtttcgctc tatcttttgc cgaaaatatc
      541 cagtttttcg atacaaaaca gtacattggc atgtcatgca aataggttct tcgtgaaatg
      601 gctaattcag gaaaatgagg aataagctgt aaccccatta ttaagccttc catactgcac
      661 acgatcttac ttaattgtcc gtaatcgcgt aaaaaagcaa tgtcagtttt tatcaaccac
      721 aacgataggc tgactaatat gaacataaag atgagataat agatcggttt taaggctaag
      781 gcgcgccgac aaagccacat taagagtgga atcgtaacga gatcaaagaa aaatgatgag
      841 tctatgatga tttccttatg tgagttgatg aataatcagg catgaaggaa accttaaaaa
      901 ggaaaagtcc cttcattacc actttttaaa accttttaag gcttaaaaat caaaagtcgt
      961 aatgaaggga ctattgtccg tgggcaaaaa ggtattagtt atagggggct ttgtcaagcg
     1021 aaaatttact gtgcattttc tcgccaaaac tgatttaagc cttgataaaa tctcgaaagt
     1081 accttgtcaa gaaaaatctt tttagtggtt tgattgctag ttttttgagc aaaactatgc
     1141 tgtaatgcag taaaaataca gtttctatct gtgtttggaa tccagttaca aattggagag
     1201 taatacaaag catatttgct tgagcatctc agcatcttgg tagcattctt ctgattttga
     1261 aatatcccaa acaagctgcc gtacacatga tgaaattctc attaacgcca ataaatctag
     1321 gctacttggc aatcttttcc ctgcaaccca atctttaact gttttctcac tcacatctaa
     1381 tgcattggcg accattttcc ccgtccaata ttgagcatgc atttcctttc gtaacgcaat
     1441 tgcaaaccgc tctttcgttt caatctcatt gagtgtaatt ttgggtaaga ttttaccctt
     1501 tgagtgcaac atatttcctc ccgattcctt tatattggtg tagataagga gtcattagaa
     1561 tggttacacg tacaaagaat caacctaaaa atcaggcggt ttcctacatt cgaatgtcaa
     1621 cggaacacca agaattttca ccagaccttc aacgttgctt tatcgaaaat tatgctaaag
     1681 aaaatgggct agaaatcatt agagaataca ttgatgaagg aaaaagtggg ctaaccgcag
     1741 aacaccgccc acaattcctt tcaatgatac aactcgtaca aagtggacaa gccgacttcg
     1801 atcatatttt ggtgtatgac gtgagtcgtt ggggtcggtt tatgaatatt gatgaatcgg
     1861 ggcattatga actgatttgt acctaccaag gcattaaagt ccactattgc gccgagccat
     1921 tcaaagggaa tgatattgcc tctcaaatat ataaaaccat gaaacgtttg tcggtaggag
     1981 actactgtcg tgaactcgga gtgaaagtat ttaacggtca aaaaaatctg atccaacatg
     2041 gatttagaca aggtggggta gctggatttg gattgcgtag gagattgatt gactgtcaag
     2101 gaaaccctaa atttgatctc caacgtggag atagaaagag cctacaaacc gacagagtga
     2161 ttttggttgc aggcccaaag gaagagcagg aaattgtccg acaaatttac catgattttg
     2221 tttatcaaca taaaacggaa caacaaattg ctgatagctt aaatgcacaa ggcattgtta
     2281 ctgatagaaa tacagcatgg acgaaaggcg tagtacatca aatccttatt aacgaaaagt
     2341 atgtcggcca caatgtttgg aataaacgta gtgcatcaaa aacacgcgaa tataaaggta
     2401 aaaaccccgt agaagagtgg gtgcgagctg agaatgtatt tgaagctatc gtgccacaag
     2461 ccttatttaa tgcagcccaa accattattc atcaacgctc cgcccgatta tctgacgatg
     2521 aaatgttaga gcaactcaag ctacttttga aagagaaagg gaagctgtct ggcttgatca
     2581 ttgatgaagc cgaaaattgt ccatcaagta gcgcgtacag tagccgattt ggaagcctac
     2641 ttaacacata tactttaatt aactatgaac ccgaacggga ttaccactat gtagagatca
     2701 accgattact ccgtcagcag cataaaaatt tcgtacaaca aacacttgat accattattg
     2761 aattgggggg agtggttttg agcgatgatg taaccgattt agtccacatc aataatgaat
     2821 ttaccgcctc aatcgtatta agtcgttgtc gcctaacgcc atcaggaagc aaaagatggt
     2881 tgatccgatt tgataccagt ttaaatcccg acataacaat tgcagtgcgc atcaatgaga
     2941 gtgccacaga aattcttgat tactacctgc ttcccacaac tgaaaaggta aatgaaaaac
     3001 tacgattggc tgagagtaat cctgccgaac ttgaaatcta ccgccatgat aatttagatc
     3061 gcttttttat tatggttgaa cgtattttag tcaaggagtt tatctatgca aaacgacacc
     3121 attcaaattc ttcctatcga caaaattcgg gtactcaatc ccagaacgcg caatcagaaa
     3181 gtctttgctg atctggtcga aaacattgca acgattggtc tcaagagacc tattaccgta
     3241 acgccatctg tcgatagtgc tgaatattat gatttgttat gcggtcaagg tcggcttgag
     3301 gcttgtcagg tgcttggtga gctattcata ccttgtcgcg ttgtgaacgc aaatagtgaa
     3361 gaaagctact tgatcagcct tgtggaaaat attgccagac gtcagcattc ctatatggag
     3421 ctgctttcag gcatcaaaat actcaatgat agaggttata agcccgttga tattgctcgc
     3481 aaaatcggtt tctcaaaaag ccatgtcaac agtatcttac acctcatcaa tgctggggaa
     3541 gagcgcctta tcaatgcggt agagcaagga attttaccca ttaccatcgc tatttcgatt
     3601 tctcgttcag acgatcagga agttcaaaaa caactcgcgg aactttacac caatggtaca
     3661 ttgaaaagca ccgatattgc caaaatacgt aacctgatcc accgtagaaa tcttgctggt
     3721 aagaaagcca atacaggtat ttctggaagt attagatcat ctatttataa tcccaaaact
     3781 gtggtgaata tctacaaaga ggaaacggaa agacagaaaa tgatgatcaa gcaagcggac
     3841 tatgatgaaa aacaactctc tattatcttg tcttgcttaa ataaactctt tgaggacaaa
     3901 tactttcaac tcgtccttaa atcagaacat ttagacgata tgccaaaaga tttaagccaa
     3961 cgaatgttaa aacaacgaga gggaaatatt tatgcctaaa catattactt atgcctttca
     4021 taatcagtta atttcgcttc caattgatga gattattcca ttaaaaaaga tggataaaaa
     4081 ttttgagaaa agcactaagt tccaaagcat tctggcttca attcgtgaaa tcgggattgt
     4141 tgaacctgtg gtcgtctatc cagaagaaaa caaccaatat attttgttag atggccattc
     4201 tcgtgtacat gcactcaaaa agctaggtgt ggataaggtg ctttgtatga tttctaccga
     4261 tgatgaaggc tttacttaca acaagcaaat taaccgctta accgccgttc aggaaaataa
     4321 aatgctgatg aaaaccattg agcgcggggt ttcggatgaa ctgatcgcaa gaacactcaa
     4381 tatcaatcta aaagcactac ggcagaaaat gaatatgctt aatgggatca caagagaggt
     4441 tgttgatata ttggccaata agcaggtcag taaagagata tttcggattt tgcgcaagat
     4501 gaagcctgaa cgccaaattg aagtggcaca aatgatgatc gccagtaata agtttacatt
     4561 gacatacgcc aatatgatgt tgctcagttc acgcaaggag gaattagtcg aaagccacaa
     4621 gtctaaaacc acgaatgatg atttaaccga tctttccatt atgcaaaaag agctagagcg
     4681 acttaaagag aactataaaa tttccgaaga gaaattggcg gacttaaaaa tgggattggt
     4741 tgtggcgaaa gggtatgtca aaaaactatt gaataatgct gccgtggtag atttgcttga
     4801 aaatgatcag agtgaaatct atcttactct taaagaagta tgtgatattc aataatataa
     4861 tatctagcta ataaccttaa
//
LOCUS       DANLUQ010000162         4814 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.163, whole genome shotgun sequence.
ACCESSION   DANLUQ010000162 DANLUQ010000000
VERSION     DANLUQ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4814
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.163"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(14..244)
                     /locus_tag="PXB27_002244"
     CDS             complement(14..244)
                     /locus_tag="PXB27_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346988.1"
                     /translation="MSLLKKAQTFISIFNEVRAKNEAQRQSSNNLNVALKNAAEMRAE
                     MNGFSLKPQKRSTYFADHYGCGIHAVETYDNE"
     gene            complement(365..682)
                     /locus_tag="PXB27_002245"
     CDS             complement(365..682)
                     /locus_tag="PXB27_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5346989.1"
                     /translation="MLSRKEVLMTRRASMNLQQIIRNVVEYTYSYESGDKLSAEIHNT
                     LDNLSYFPRIGVIGSVENTRECFCRGYRIIYRETENQVQILAIIHCRQQYPTDYYRSA
                     FKA"
     gene            complement(675..833)
                     /locus_tag="PXB27_002246"
     CDS             complement(675..833)
                     /locus_tag="PXB27_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346990.1"
                     /translation="MTEEYQNFLIEQVKQGLDDIENGRVISLEESRQLCQKTIYETLQ
                     ELQGAVYA"
     gene            complement(887..1042)
                     /locus_tag="PXB27_002247"
     CDS             complement(887..1042)
                     /locus_tag="PXB27_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346991.1"
                     /translation="MLFKEKGYDEFLTQKIAKGRAELESGKGIPLEQATLLVQSTIEE
                     TAQELDD"
     gene            complement(1180..1437)
                     /locus_tag="PXB27_002248"
     CDS             complement(1180..1437)
                     /locus_tag="PXB27_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346992.1"
                     /translation="MICHADPEYVKYNYAGLWEKGSDGTFFYNYQQFMPEVEAKESLR
                     EQEPELDDGYNEEEYSEEEYSDENDASDENDNYDETYDDNF"
     gene            1451..1627
                     /locus_tag="PXB27_002249"
     CDS             1451..1627
                     /locus_tag="PXB27_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346993.1"
                     /translation="MAYVRIYQGEAYGWCAEKGEANTECPEALLVSLTGDIFEAVGGD
                     KLKGAKKWQPYKKE"
     gene            1631..1777
                     /locus_tag="PXB27_002250"
     CDS             1631..1777
                     /locus_tag="PXB27_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346994.1"
                     /translation="MDTTQKRVALFIIGLSVVMWLRKHNCCTVIFVLLLAALAFSYTM
                     EHLY"
     gene            1793..1999
                     /locus_tag="PXB27_002251"
     CDS             1793..1999
                     /locus_tag="PXB27_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346995.1"
                     /translation="MVKNVVHEEILHNRAELQQAVEKEEFCAKCDNSVVFHLRDNYHE
                     FTLGLDTILQCLHFAEEKGAIRIP"
     gene            1996..2772
                     /locus_tag="PXB27_002252"
     CDS             1996..2772
                     /locus_tag="PXB27_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346996.1"
                     /translation="MNIEYSLNHCQGDGVAFYGRLGTDDLIQLFNHLNPQKRKQKMFA
                     NLLTHIESWETHDDIGFEIIRNRFGYRYSHWNTMELYAKTADQLVFFNDSDARRNWYF
                     PTSKVYKYKGLWDEFIQDLEQYIKGTSREMESVGYQIIEASPYQTETVFKFNTENYLV
                     ELKRKPSEFYSELVDWAFGDMCDFEATIEQICQGQLCYADFEATVTDKKSEIQLGLAR
                     LSGISYSPNDRSFSGYRQELIGEAIASARHNAARFAYLHA"
     gene            2887..3299
                     /locus_tag="PXB27_002253"
                     /pseudo
     CDS             2887..3299
                     /locus_tag="PXB27_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484619.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
     gene            3313..3468
                     /locus_tag="PXB27_002254"
     CDS             3313..3468
                     /locus_tag="PXB27_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346997.1"
                     /translation="METRIVKVIYDDGDYFFTRINADEQAIRNYYAIGSSIGFDDIRK
                     IIAVEIL"
     gene            3548..4522
                     /locus_tag="PXB27_002255"
     CDS             3548..4522
                     /locus_tag="PXB27_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1738 domain-containing protein"
                     /protein_id="HDL5346998.1"
                     /translation="MNTQPKFDLYQHITDRIVVALEQGTAPWLKPWSHPECNMALPRN
                     AVSNRLYSGINTLLLWIATAEHGYKQCKWITAKKANELGGYIRKGEKATVVMNYRPDE
                     REKLDEEGNIVYDEEGNPEMERHFFINKHNLFNLDQCENLPVELYDPVSEPKQPKSEP
                     NQYDIFAEIRQIIKGMDLQVEMKPSNRAFYQPGEDKVVMPEMKQFANAQAFHSVLLHE
                     MTHATGANNRLNREGITSGKAKFGNKVYAFEELIAEMGGAFLCAYLGFDTVPQNAAYI
                     ESWIRVLKEDKRAIVRASGKAREACQYMLDALNVAQLADKYDVFEEAA"
     gene            4609..4806
                     /locus_tag="PXB27_002256"
     CDS             4609..4806
                     /locus_tag="PXB27_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5346999.1"
                     /translation="MTKSTEIPRGHDNWVITGRDNDGKWHKERFLYYSRREAIQQFRR
                     KHNCVGKHSVIESITPQFGWY"
BASE COUNT         1436 a          845 c         1010 g         1523 t
ORIGIN      
        1 tagttaccgc tatttattca ttatcatagg tttctactgc atgaatacca caaccataat
       61 ggtcagcaaa gtaagtagaa cgcttttgag gcttaaggga gaacccattc atctcagctc
      121 tcatttcagc agcatttttc aaggcaacat ttaaattgtt tgaagactgg cgttgagctt
      181 catttttagc tcgcacttca ttaaaaatag agataaacgt ttgtgctttt ttaagtagag
      241 acattgtggt tctcctgttt tttgcaccta atattattga actatggtag gcttggcgag
      301 cattgccttg agttttaatt cggctacggt catttttctt tttatttcca ttttatagct
      361 cctgttatgc tttgaatgca gagcgataat agtcggttgg gtattgttga cgacaatgga
      421 taatggctaa aatttgcact tgattttctg tttctcgata tataattcta taacctcggc
      481 aaaagcattc tctagtattt tctactgaac ctataacacc aattcgtgga aaataagaaa
      541 gattatccaa agtgttatga atttccgcag ataatttatc accactttca tagctataag
      601 tatattccac aacattacga atgatttgtt gtaaattcat acttgctctg cgagtcatta
      661 acacctcttt tcgactaagc atacacggct ccctgtagct cttgcaaggt ttcatatatg
      721 gttttttgac acagttgacg gctttcttct aatgaaataa cacgtccgtt ttctatatca
      781 tcaagtcctt gttttacttg ctctattaag aagttttggt attcttctgt catggataac
      841 tcctatcatt agttgtatat agatatatta gcaatgttac aaattgttaa tcgtccagtt
      901 cttgagcagt ttcttcaata gttgattgaa ctaacaatgt ggcttgctca agaggaatac
      961 cttttccgga ttctaattca gctctgcctt ttgcaatttt ttgagtaagg aactcatcgt
     1021 agcctttttc tttaaatagc attttatgtt cctttcactt attacaaggc tttcaggcat
     1081 agggattatg ttctcctaaa tgcgaaatat tggaataatt tacgcttaaa gcaatagagt
     1141 gtcaagagca gggtttttgg aagtgtggtt tgggaatatt taaaaattat catcataagt
     1201 ttcatcgtag ttatcgtttt catcgctggc atcattttca tcggaatatt cttcttcgct
     1261 atattcctct tcgttatatc catcatcaag ttcaggttct tgttccctta gtgattcttt
     1321 agcttcaact tcaggcataa attgctggta attgtagaaa aaagtgccat cgctgccttt
     1381 ttcccaaagt ccggcgtagt tgtattttac atattcagga tcagcgtggc atatcatccc
     1441 taattgaggg gtggcatatg ttcgtattta ccaaggtgag gcttatggtt ggtgtgctga
     1501 aaaaggagag gcaaatacag aatgccctga ggccttattg gtgagtttaa ccggcgatat
     1561 ttttgaagca gtcggcggag ataagctgaa aggtgccaaa aaatggcagc cttacaagaa
     1621 agaatgacag atggatacga cacaaaaacg tgtggcttta ttcatcattg gattatctgt
     1681 tgtgatgtgg ctaagaaagc ataactgctg tacagtgata tttgtattgt tgttggcagc
     1741 gttagcattt agctacacga tggaacattt atattagctg gggaactgaa ttatggtaaa
     1801 gaatgtggtt catgaagaga tattacataa ccgtgcagaa ttacagcaag cggttgaaaa
     1861 agaagaattt tgtgcaaaat gtgataactc agtagtattc catttacgtg ataattatca
     1921 cgaatttacg cttgggttag atactatatt gcagtgtctg cattttgctg aagagaaggg
     1981 agctatccgg ataccttgaa tattgagtat tcactcaatc attgccaagg agatggagtc
     2041 gcattttatg gtcgacttgg tacagatgat ctaatccagc ttttcaatca tctcaatcca
     2101 cagaaacgta aacagaaaat gtttgctaat ttgctaacgc acattgagtc ttgggaaacc
     2161 cacgatgata ttggttttga aattatacga aatcgctttg ggtatcgtta ctcgcattgg
     2221 aatacgatgg agctttatgc taaaactgca gatcagttag tgtttttcaa tgatagtgat
     2281 gctcgaagaa actggtattt tccgacttca aaagtgtata aatacaaagg cttgtgggat
     2341 gagtttattc aggatttaga acagtatatt aagggtactt cccgtgaaat ggaatcggtc
     2401 ggttatcaaa ttattgaagc ctcgccgtat caaacggaaa ccgttttcaa atttaatacg
     2461 gaaaactatc tagtagaact gaaacgtaaa ccttctgagt tttattcaga gcttgttgat
     2521 tgggcatttg gtgatatgtg tgattttgaa gcaactatag agcagatttg tcaagggcaa
     2581 ctctgttatg ctgattttga agcaactgtt accgataaga aaagtgaaat tcagttaggt
     2641 cttgctcgct tgagtggaat aagttactcg ccgaatgacc gttcgtttag tggttatcga
     2701 caagagctta ttggtgaggc gattgcctct gctcgccata atgcagcacg ttttgcctac
     2761 ttgcacgcct aatttgcaga tttgtacttc aatttgaccg cttgtagtgg ttaattttca
     2821 ccccgtgggg aaatattttc cctattgggc aagatgtttc tccacttttt aatttggaga
     2881 aatgtgatga aaaatgaaca caatttagag caacttaaat atatttggga taaccgcaac
     2941 cttgaaaata aagcctatca atacctgttt cacatttgca ttgagccgat gaaaaaagga
     3001 gattggaatc gttatacgat ttggcttaat tttcacggca acttaatcaa cgtgtcagac
     3061 gttgtatatc gagttgtgga atctcaaaag ctcaaggatg acatcaaaat gtggtatgac
     3121 aataatacca ttgtcgtccg ataaaagttg aaacaacagg cttcatctca tatttactgc
     3181 accagttcta tcaggggata gcctatattt tactggaaca gggaatgatt actgaaactg
     3241 aagcagccga gaaaagttat aacttcagag ctgcaaattg ttttcaacgt ttgctttaat
     3301 ggaggtgaga taatggaaac tcgcattgtt aaagttattt acgatgatgg tgattatttc
     3361 tttacccgaa tcaatgctga tgaacaggct atccgtaatt actacgccat aggcagttca
     3421 ataggttttg atgatataag aaaaattatc gctgttgaaa ttttataaac cacccatagg
     3481 ggaaactatt tccccatagg gtgatagcgt ttccccattt ttctacccga actttggaga
     3541 aacacttatg aacactcaac ctaaatttga tttataccaa cacatcactg accgaattgt
     3601 tgtagcactg gaacaaggta cagccccgtg gttaaaaccg tggagccatc cggaatgcaa
     3661 tatggcatta cctcgcaatg cggtatctaa ccgcttgtat tccggtatca atacactctt
     3721 actctggatt gctactgctg aacatggtta caaacagtgc aagtggatta cagcaaaaaa
     3781 agccaatgaa ctcggcggat atatccgtaa aggggaaaaa gctactgtgg tgatgaacta
     3841 ccgtcctgat gagagagaga aactcgatga ggaagggaat attgtgtatg acgaggaagg
     3901 aaatccggaa atggaacgac actttttcat taataagcac aatctgttca atctggacca
     3961 gtgtgaaaat ttaccggtgg aactgtacga tcctgtatca gaaccgaaac aacctaaatc
     4021 tgagccaaac caatacgata tttttgctga gattcggcag attatcaaag gaatggattt
     4081 gcaggtggaa atgaagccta gcaatcgggc attttatcaa ccaggtgaag ataaggtcgt
     4141 aatgccggag atgaagcagt ttgcaaatgc tcaagcgttt catagtgtat tgttacacga
     4201 aatgactcac gcaacggggg caaataatcg gcttaatcgg gaaggtatta cttccggaaa
     4261 ggctaaattt ggaaacaaag tctatgcttt tgaggagttg attgctgaaa tgggcggtgc
     4321 atttttatgt gcctaccttg gttttgatac tgtgcctcaa aatgcggcgt atattgaaag
     4381 ttggattcga gtattgaaag aagataaacg ggcgattgtc cgtgcttccg gaaaagctcg
     4441 tgaagcctgc caatacatgt tagacgcatt aaacgtcgca cagttggctg acaaatacga
     4501 tgtatttgaa gaagctgctt aacaaatttg ccgaaaggca tatctcaagg ggagacctat
     4561 ccctttgggc atagtgtttc cctatttttt attaaatgga gaaaagctat gacaaaatca
     4621 actgaaatac ctcgtggaca tgataactgg gttattacag gcagagacaa tgatgggaaa
     4681 tggcataaag aacgttttct ttattattct cgccgtgaag ccatccaaca gtttcgtcga
     4741 aaacacaact gcgtaggtaa acatagtgtt attgaatcaa taacacctca atttggctgg
     4801 tattaaccgg cttt
//
LOCUS       DANLUQ010000163         4789 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.164, whole genome shotgun sequence.
ACCESSION   DANLUQ010000163 DANLUQ010000000
VERSION     DANLUQ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4789
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.164"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..446
                     /locus_tag="PXB27_002257"
     CDS             <1..446
                     /locus_tag="PXB27_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HDL5347000.1"
                     /translation="TVNAAPVELHQFDSPKQESDYRALIQELRCPQCQNNNIADSNAT
                     IATDMRAKTLELLKQGKSKDEVVDYMIERYGNFVTYDPPLTPATIMLWLLPLILIALG
                     LGVVLRRKKNKPQAVNFEQNTAKPTTHLAQDEQERLNQILNKEEK"
     gene            443..1354
                     /gene="ccmI"
                     /locus_tag="PXB27_002258"
     CDS             443..1354
                     /gene="ccmI"
                     /locus_tag="PXB27_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HDL5347001.1"
                     /translation="MNFWLAIIFVTLIVCGVAFYPLLKKNKQIDNTKRDSLNKAFYFD
                     RLKEVENEANEGVIDDPEQTKLELQKNLLDDIPHSTQSATSEKLTLGKSWFVALILFV
                     GGISFAIYSNVGSWFSNSMLDTTHQKLDSFYERLKTEETNPLSQEEMNQFAMALRVDL
                     QSNPNDDKKWYMLGQIGMALDDGQLAHDAFSKAAQLKPDNLDYKLSHAQLLLFSEAQE
                     DKKQGETLLKEIIRQDHTNFNALSLLAFHSFEKEDFKMAAMTWGMMLKLLPDEDPSKV
                     TVERSFNMALSNLSEAEKQELQKGEKK"
     gene            1351..1956
                     /gene="mog"
                     /locus_tag="PXB27_002259"
     CDS             1351..1956
                     /gene="mog"
                     /locus_tag="PXB27_002259"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HDL5347002.1"
                     /translation="MTACKPLSKEKLKIGLVSVSDRASQGVYSDQGIPELRAWLESAL
                     TEPFELETRLIPDEQAIIEQTLIELVDAHACHLVLTTGGTGPAKRDVTPDATLVVAHR
                     EMPGFGEQMRQVSLHFVPTAILSRQVGVIRHDSLILNLPGQPKAIKETLEGVKDEQGK
                     VLVKGIFSAVPYCLQLLSEIYVETHAAICESFRPKSARREN"
     gene            1966..2304
                     /gene="glnB"
                     /locus_tag="PXB27_002260"
     CDS             1966..2304
                     /gene="glnB"
                     /locus_tag="PXB27_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005654045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HDL5347003.1"
                     /translation="MKKIEAIIKPFKLDDVREALTDVGVSGMSITEIKGFGRQKGHTE
                     LYRGAEYAIDFLPKVKVEIVIPDELEEQCIEAIMETAQTGKIGDGKIFVYDVGRVIRI
                     RTGEENEEAI"
     gene            2385..2864
                     /gene="lspA"
                     /locus_tag="PXB27_002261"
     CDS             2385..2864
                     /gene="lspA"
                     /locus_tag="PXB27_002261"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005724521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDL5347004.1"
                     /translation="MKTGLKFLWVSLIVIIIDLWSKYIVVQHFEFGESINILPIFNFT
                     YARNYGAAFSFLADHSGWQKYFFLGLAVVISSALVVALWKNQAVKKLENIAYALIIGG
                     AIGNAIDRAYNGYVVDFLHFYWDIYHYPVFNIADIAICVGAGLLILEAFITKKEPKK"
     gene            2875..3819
                     /gene="ispH"
                     /locus_tag="PXB27_002262"
                     /gene_synonym="lytB"
     CDS             2875..3819
                     /gene="ispH"
                     /locus_tag="PXB27_002262"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HDL5347005.1"
                     /translation="MKILLANPRGFCAGVDRAISIVELALEIHGAPIYVRHEVVHNKF
                     VVDGLKAKGAIFVEELHEVPDDAIVIFSAHGVSQVVRQEAKNRGLKVFDATCPLVTKV
                     HMQVARASKKGTKAILIGHEGHPEVIGTMGQYNNQEGGIYLVESVEDIANLPVSNNDE
                     LTFMTQTTLSIDDTIGVIEALKEKYPAIQGPRKNDICYATTNRQHAVRELAEQSQLVL
                     VVGSKNSSNSNRLAELASRMGVMSKLIDGPQDIDPTWLEGIETIGITAGASAPEVLVQ
                     SVISHLKTLGVTEVETLVGNEEDVVFEVPKELRIKEVK"
     gene            3819..4067
                     /locus_tag="PXB27_002263"
     CDS             3819..4067
                     /locus_tag="PXB27_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HDL5347006.1"
                     /translation="MTEINRFKLEWACRRGMRELDKMIMPFYQNHFNELNETQQQAFV
                     EMLNYSDPELFRWVMLQAPAPTEEIAELIRLMRDKIET"
     gene            complement(4274..>4789)
                     /locus_tag="PXB27_002264"
     CDS             complement(4274..>4789)
                     /locus_tag="PXB27_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL5347007.1"
                     /translation="SANQYIFNSDWFTHNVPSLASIFEYVKPRRILEIGSFEGRSSVF
                     FIEEALKYQSQVEIHCIDSWEGGAEHQGVWDMGEIEQRFIHNVTLAQQKFQNVVLYKH
                     RGYSHQKMIELLANGYINYFDYIYIDGSHEAPDVLFDALLAHRLVRKGGVISFDDYLW
                     SPDETGKQRHY"
BASE COUNT         1506 a          968 c         1038 g         1277 t
ORIGIN      
        1 taacagttaa tgctgctccg gtagaactgc accaattcga cagcccgaaa caagagtcgg
       61 actatcgtgc cttaattcaa gaactgcgtt gcccgcagtg tcaaaataat aatatcgccg
      121 attccaatgc gactattgcc accgatatgc gggcaaaaac cttagaattg ctcaaacaag
      181 gcaaaagcaa agacgaagtg gtggattata tgattgaacg ctacggcaat ttcgtcactt
      241 acgacccgcc tctcacaccg gcaaccatta tgttatggct gttgccgtta attttaattg
      301 ccttagggct gggggtggtg ttgcgtagaa agaaaaacaa accacaagcg gtcaattttg
      361 agcaaaatac tgcaaaaccg accactcatc tcgctcaaga tgagcaagaa cgtcttaacc
      421 aaattctcaa taaggaagaa aaatgaattt ctggctggca attattttcg ttacactgat
      481 tgtatgtggc gtggcgtttt atccgttact taaaaagaac aagcaaatcg ataacaccaa
      541 gcgagacagc ttaaacaaag ccttctattt tgaccgctta aaagaagtgg aaaacgaagc
      601 gaatgaaggg gtgattgatg accctgaaca aaccaaatta gagctacaaa aaaacttgct
      661 tgatgatatt ccacacagca cacaaagtgc cacaagtgaa aaattaacgc tcggcaagtc
      721 gtggtttgtg gcattgattt tatttgtcgg cggtatcagc tttgcgattt acagcaatgt
      781 cggttcttgg ttctccaact caatgttaga caccactcac caaaaattag acagcttcta
      841 tgagcgttta aaaaccgaag agaccaatcc gctctcacaa gaggaaatga accaatttgc
      901 gatggcatta cgggtagatt tacaaagtaa tccgaatgac gacaaaaaat ggtatatgct
      961 cggtcaaatc gggatggcgt tagatgatgg tcagcttgca cacgatgcct ttagcaaagc
     1021 ggctcaactc aagccggata acttagatta caaactcagc cacgcccaac tgctgctctt
     1081 ttccgaagca caggaagaca aaaaacaggg tgaaaccttg ttaaaagaga ttatccgcca
     1141 agaccacaca aactttaatg cgttaagttt attggcgttc cactcctttg aaaaagaaga
     1201 tttcaaaatg gcggcaatga cgtggggaat gatgttaaaa ctactgcctg atgaagatcc
     1261 tagcaaagta acggtagaac gcagttttaa tatggcgtta tccaatttaa gtgaggcgga
     1321 aaaacaagaa ttgcaaaaag gtgagaaaaa atgaccgctt gtaaaccact tagcaaagag
     1381 aagttgaaaa tcggcttggt gtcggtttca gaccgagcct cgcagggggt ttattcagac
     1441 caaggcattc ccgaattaag ggcgtggctt gaatctgcct taaccgagcc gtttgagctg
     1501 gaaacccgct taattcccga tgagcaagcc attattgagc aaaccttaat cgagttagtc
     1561 gatgctcacg cttgccattt ggtattaacc accggcggca ccggcccggc aaaacgtgat
     1621 gtcaccccag atgccaccct tgttgtcgcc caccgagaaa tgcccggctt tggcgaacag
     1681 atgcgacaag tgagccttca cttcgtgcca accgccattc tctctcgcca agtgggagta
     1741 attcgccacg acagtctgat tttaaatctg ccggggcaac cgaaagcaat taaagaaacg
     1801 ctggaaggtg tgaaagatga acagggcaaa gtattggtta aaggtatttt tagtgccgtg
     1861 ccatactgtt tacaactttt aagcgaaatt tatgtcgaaa cccacgcagc tatttgcgaa
     1921 agtttcagac ccaaatcggc aagacgagaa aattaaggct aaaaaatgaa aaaaatagaa
     1981 gcaattatta aaccttttaa attagacgat gtgcgtgaag ccttaaccga tgtgggtgtc
     2041 agcggaatga gtattaccga aatcaaaggg tttggcagac aaaaaggaca taccgagctt
     2101 tatcgtgggg cggaatatgc gattgatttt ctaccgaaag tgaaagtcga aatcgtgatt
     2161 cccgacgagt tggaagaaca gtgcattgaa gcaattatgg aaaccgccca aaccggcaaa
     2221 atcggcgatg gcaagatttt tgtgtatgat gtcgggcggg tgatccgcat tcgtaccggc
     2281 gaagaaaatg aagaagcgat ttagttttgc aaaaaatccg gcaaaaacga ccgcttgcag
     2341 ggggatttcc atctactctt tttacattaa attttggata ttaaatgaaa acaggcttaa
     2401 aatttctttg ggtgagttta attgtaatca tcattgacct ttggtcaaaa tacattgtag
     2461 tgcaacattt tgaatttggt gaaagcatta atattttgcc gatttttaac ttcacttacg
     2521 cccgaaacta cggggctgcc tttagtttcc ttgccgacca ttccggctgg caaaaatatt
     2581 tctttttagg gctggcggta gtgatttcca gtgctttagt ggtggctctt tggaagaatc
     2641 aagcggtcaa aaaattagaa aatattgcgt atgccttaat tattggtgga gcgattggga
     2701 atgccattga ccgtgcttac aacggctatg tagttgattt ccttcatttt tattgggata
     2761 tttatcacta ccctgtgttt aatattgccg atattgcgat ttgtgtgggt gcaggcttac
     2821 tgattttaga agcctttata accaaaaaag aaccgaaaaa ataagagtga ttaaatgaaa
     2881 attttattag caaacccacg cggtttttgt gccggcgtgg atcgagccat ttcgattgtt
     2941 gaattagcct tagaaattca cggtgcacct atctatgttc gccacgaagt ggtacataac
     3001 aaatttgtgg tggatggctt gaaagccaaa ggggctattt ttgttgagga gctacacgaa
     3061 gtgccggacg atgctatcgt gatcttctca gcccacgggg tgtcgcaagt agttcgccaa
     3121 gaagccaaaa atcgtggctt aaaagtgttt gatgccactt gcccactcgt gaccaaagtg
     3181 catatgcagg ttgcccgagc cagtaaaaaa gggacaaaag caattttaat cggtcacgaa
     3241 ggtcaccctg aagttatcgg cacaatggga caatacaata accaagaagg tggcatttat
     3301 ttggtagaat cggtggaaga tattgccaac ttgccggttt ccaacaacga tgaattaact
     3361 tttatgacgc aaaccacact ttccattgac gataccattg gcgtgattga ggcgttaaaa
     3421 gagaaatatc cggcaattca aggccctcgc aaaaatgata tttgttatgc taccaccaat
     3481 cgccaacacg cagtgcggga actggcagaa caatcacaat tagtgttagt ggtaggttca
     3541 aaaaactcct cgaactcaaa tcgtttggcg gaactcgcct ctcgaatggg tgtgatgtca
     3601 aaactgattg atggtccgca agatattgat ccaacttggt tggagggtat tgaaactatc
     3661 ggtattaccg ccggtgcttc cgctcctgaa gtgttggtgc aatcagtaat ttcgcattta
     3721 aaaacccttg gcgtaaccga agttgaaacc ttagtcggca acgaagaaga cgtggtgttt
     3781 gaagtcccga aagagttacg catcaaagag gtaaaataat gacagaaatc aaccgcttta
     3841 aattagaatg ggcgtgccga cgtggtatgc gtgagttaga caaaatgatt atgccgtttt
     3901 accaaaatca ttttaatgaa ctcaatgaaa cccaacagca agctttcgtt gaaatgctca
     3961 attacagcga cccggaactg tttcgctggg taatgctgca agcacctgcc ccaaccgaag
     4021 aaatcgctga attgattagg ttaatgcgag ataaaattga gacctagagc taatagatta
     4081 acctcttctt tggggtaaat atatagctac ttaccccaaa gaagactaaa ttcattcaac
     4141 ttttacaaaa taaccctaaa ctagaaaagt taatcttcca atttaataat atgttgctga
     4201 taagctatta aaccttgtat tacatgcact ttctgctgat aggtattgac atagtgatca
     4261 acagcgggtt ttactaataa tgacgctgtt tacctgtctc atctggtgac caaaggtaat
     4321 catcaaaact aataacgcct ccttttctca ctaaacgatg tgcaagtaaa gcatcgaaca
     4381 acacatctgg cgcttcgtga gatccatcta tgtaaatata gtcaaaataa tttatatatc
     4441 catttgcgag aagttcaatc attttttggt gagaatagcc acggtgctta tataacacaa
     4501 cattttggaa tttctgttgc gcaagagtga cattatggat aaatcgttgt tctatttctc
     4561 ccatgtccca aactccttga tgctctgctc ccccttccca actatcaata caatgaattt
     4621 caacttgtga ttgatatttc aatgcttctt caatgaaaaa gacagatgat ctaccttcaa
     4681 atgatccaat ttctaaaatc ctccgaggtt ttacatattc aaaaatagaa gctaatgaag
     4741 gaacattatg agtaaaccaa tcagagttaa aaatatattg gttggctga
//
LOCUS       DANLUQ010000164         4742 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.165, whole genome shotgun sequence.
ACCESSION   DANLUQ010000164 DANLUQ010000000
VERSION     DANLUQ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4742
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.165"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            51..380
                     /gene="trxA"
                     /locus_tag="PXB27_002265"
     CDS             51..380
                     /gene="trxA"
                     /locus_tag="PXB27_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018357021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDL5347008.1"
                     /translation="MSNVIHTTDATFEQDVLKSDVPVLLDFWAPWCGPCRMIGPVLDD
                     LSVDAQFDGKVKIVKMNVDENPSVPAQFGVRSIPFLLLFKNGEVIAQQVGAIPHAQMA
                     EFIKQAL"
     gene            551..1774
                     /gene="hflK"
                     /locus_tag="PXB27_002266"
     CDS             551..1774
                     /gene="hflK"
                     /locus_tag="PXB27_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HDL5347009.1"
                     /translation="MSWNESGNQQDPWGKPGQKKAEPKPEQQEQGSQQEPTNNQRNEQ
                     QPPDLEEVFSSLLKKMGGGKGPNNSSNQPSASLGKFLPVVLGLAAVVWVGSGFYTVQE
                     AERGVVTRLGKLNDIVLPGLNWKPTFIDSVTRVNVERVSELNTSGSMLTQDENMVQVE
                     MTVQYRVEDPAKYLFSVSNPDDSLKQATDSALRYVIGHMTMDEILTTGRATVRERTWS
                     TLRDIIKTYDMGLLVTDVNFQYARPPEEVKAAFDDAIKAQEDEQRLIREAEAYARGEE
                     PIARGQAQRTIEQAQAYKEAVVLNAKGEVERLSQLLPEYKASPELTRERLYIQTMEKV
                     MKNTPKVVMDSSGNNLNVLPFDKLMNSSSVIKAEQPAAAQVVTPQVVQPQVQPRTQIQ
                     PQQAEQQEPTRKGRF"
     gene            1777..2664
                     /gene="hflC"
                     /locus_tag="PXB27_002267"
     CDS             1777..2664
                     /gene="hflC"
                     /locus_tag="PXB27_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HDL5347010.1"
                     /translation="MRRLLVPILAVVAFVVLQAITIVNEGERGIMLRFNKVHRDSDQK
                     VVVYEPGIHFKVPFIDSLKVLDARIQTLDGQEDRFVTVEKKDLLVDSYVKWRISDFGQ
                     FYTSTGGDYQKAADLLRRKVGDRLRSEIGSRTIKDIVSGSRGELMAGAQKALNAGEDG
                     AERLGIEVVDVRVKQINLPNEVSSSIYQRMRAERDAVAREHRSQGNEKAEVIRAEVDK
                     KVVLILANANKTAQALRGEGDAQAAKLYSEKFGNEPEFYSFIRSLKAYEDSFAEGQNN
                     MMLLKPNSEFLRFMQAPTK"
     gene            2838..4136
                     /gene="purA"
                     /locus_tag="PXB27_002268"
     CDS             2838..4136
                     /gene="purA"
                     /locus_tag="PXB27_002268"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010869263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDL5347011.1"
                     /translation="MGKSVAILGAQWGDEGKGKIVDLLTDRVKYVVRYQGGHNAGHTL
                     IINGEKTVLRLIPSGILRDNVTCLIGNGVVLSPEALMKEMGELEERGINVRERLKISE
                     ACPLILPYHVAMDHAREAALGKNKIGTTGRGIGPAYEDKVARRGLRVSDLFDKEHFAA
                     KLKDILDYYNFQLVNYYKVEAVDYQKTLDDVFAIADVITGMVADVTTLLHEARENGDN
                     ILFEGAQGTMLDIDHGTYPFVTSSNTTAGGVATGSGYGPRHLDYVLGIIKAYCTRVGS
                     GPFTTELFDDVGAEIARKGNEFGAVTGRPRRCGWFDAVAVRRAVQINSISGFCMTKLD
                     VLDGFKELKICVAYKMPNGKIVEYAPLAAKDWEGVEPIYETMPGWSENTFRVTKREDL
                     PQAALDYIKRIEELVGVPVDILSTGPDRVETMILRDPFAA"
     gene            complement(4296..4366)
                     /locus_tag="PXB27_002269"
     tRNA            complement(4296..4366)
                     /locus_tag="PXB27_002269"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4336..4338),aa:Arg,seq:tct)
     gene            complement(4515..>4742)
                     /locus_tag="PXB27_002270"
     CDS             complement(4515..>4742)
                     /locus_tag="PXB27_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347012.1"
                     /translation="DGSLNAKYVVGTGDDTDGEVTNFVIITSEYKYIDAQSAKEITDA
                     PLTKEDIGKTPTQIMLGRIYKYLKETGQIVV"
BASE COUNT         1450 a          856 c         1089 g         1347 t
ORIGIN      
        1 cttgccagtt agggcgtttc tttttttatt taatatttaa ggataaactt atgagcaatg
       61 tcattcatac aacagatgct acatttgaac aagatgtttt aaaatcagat gtaccggtat
      121 tattagactt ctgggcgcct tggtgtggtc cttgccgtat gatcggcccc gtacttgacg
      181 atttatctgt tgatgcacaa tttgatggta aagtaaaaat tgtcaaaatg aacgtagatg
      241 aaaatccatc tgtaccagca caatttggcg tacgcagcat tccattttta ctattattca
      301 aaaatggtga agtgattgcc cagcaagttg gtgcaattcc gcacgcccaa atggctgaat
      361 ttatcaaaca agcattataa ttttaagagc aatactaggg cgaatttatc gccctatttt
      421 tttgcaaata aatagtgaaa aaagaccgct tgtttcagat aaacctagtc aaaagcttac
      481 taaataggta caatcactaa cttgcattct aagctttaaa gtgcgattcg attttatttt
      541 ggagatattt atgtcgtgga atgaatcagg caatcagcaa gatccttggg gtaaaccggg
      601 gcagaaaaaa gctgagccaa agcctgagca acaagaacag ggttcacaac aagagccgac
      661 caataatcaa agaaatgaac aacagccccc tgatttagaa gaagtattta gcagtttatt
      721 aaaaaaaatg gggggtggaa aagggcctaa taatagttca aatcaaccta gtgcaagctt
      781 agggaaattt ctacctgtgg ttttaggtct tgcagcagta gtttgggttg gctcaggttt
      841 ttacactgtg caagaggctg aacgtggtgt ggttactcgt ttaggtaagc tgaatgatat
      901 cgtgttgcca gggttgaatt ggaaaccgac ttttattgat tcggttacaa gagtaaacgt
      961 agaacgtgtt tcagagctga acaccagtgg ctcaatgctg actcaagatg aaaatatggt
     1021 gcaggtagaa atgacggttc agtatcgtgt ggaagacccg gctaaatatc tgtttagtgt
     1081 gagcaaccct gatgacagct taaaacaagc aacagacagt gccttgcgtt atgtgattgg
     1141 tcatatgacg atggatgaaa ttttaaccac agggcgtgca accgtgcgtg aaagaacgtg
     1201 gtcgacattg cgtgatatta tcaaaaccta tgatatgggt ttattggtaa cagacgtaaa
     1261 cttccaatat gcacgaccgc ctgaagaggt gaaagccgcc tttgatgatg caatcaaagc
     1321 acaagaagat gagcaacgct taattcgtga agcggaagcc tatgcaagag gtgaagagcc
     1381 gattgcacgt ggccaggcac agcgtacgat tgagcaagct caagcttaca aagaagcagt
     1441 tgtgttaaat gcgaaagggg aagtggagcg tttgagtcaa ttattacctg aatataaagc
     1501 ctctccagag ctgactcgtg aacgtttata tatccagaca atggaaaaag tcatgaaaaa
     1561 tacgccgaaa gtggtgatgg attcaagtgg taataatttg aatgtattgc ctttcgataa
     1621 gctaatgaat tcttcatcgg taataaaagc ggagcagcct gcggcggcac aagtagttac
     1681 accacaagtt gttcagccgc aagtacagcc aagaacacaa attcagccgc aacaagcaga
     1741 acaacaagaa ccaactcgta aggggagatt ttaatcatgc gtagattatt agtacctata
     1801 cttgctgtag ttgcttttgt tgtgctacag gcaatcacca ttgtaaacga gggcgagcgt
     1861 ggcattatgt tacgttttaa caaagttcat cgtgatagcg atcaaaaagt ggtggtatat
     1921 gaaccgggga ttcactttaa agtaccattt atcgatagtt taaaggtatt agatgctcgt
     1981 attcaaactc tagatgggca agaagaccgt tttgtaacag tcgagaaaaa agatttatta
     2041 gtggactcct atgtaaaatg gcgaatcagt gattttggcc aattttatac ctctactggg
     2101 ggggattacc aaaaagcggc ggatttatta cgccgtaagg taggggatcg tctgcgttcc
     2161 gagattggtt ctcgaaccat taaagatatt gtatccggct ctcgtggtga gttaatggca
     2221 ggagcccaaa aagcgctgaa tgccggtgaa gatggagcag agcgtttagg aattgaagtg
     2281 gttgatgtgc gagtgaagca aatcaactta ccaaatgaag tttcttcttc aatttaccaa
     2341 cgtatgcgtg ccgagcgtga tgcggttgct cgtgaacacc gctcacaagg taatgagaaa
     2401 gcagaggtaa ttcgtgccga agtggataaa aaagtggtgc tgattttggc aaatgcgaac
     2461 aaaactgccc aagccttgcg aggtgaaggt gacgctcaag ctgcaaaact ctattcagag
     2521 aaatttggta acgagccgga attttacagt tttattcgta gtttaaaagc ctatgaagat
     2581 agctttgccg aaggtcaaaa taatatgatg ttattaaagc caaacagcga attcttacgc
     2641 tttatgcaag caccgacaaa ataattgtat ctataaaacc cgatcttgta tcgggttttt
     2701 aattttcatc cctattctga tcataacaag cggtaaattt tggctgattt tttgcaaaaa
     2761 atttgaaaaa attgaccgct tgcgatatga agttataatg ggtttcgtta ttcatcctaa
     2821 ctaaaagagg catttctatg ggtaaaagtg ttgctattct tggcgctcag tggggcgatg
     2881 agggaaaagg caaaattgtt gatttattaa cagatcgtgt gaaatatgta gtacgttatc
     2941 aaggtggaca taatgcaggg cataccttaa tcattaatgg tgaaaaaacc gttctgcgtt
     3001 taattccatc gggtattttg cgtgataacg tgacttgttt aatcggtaat ggtgtagtgc
     3061 tttctccaga ggcattaatg aaagagatgg gggagctaga agaacgaggc attaatgtgc
     3121 gtgagcgttt aaaaatttct gaagcttgcc cgttaattct gccttatcac gttgcgatgg
     3181 atcacgctcg agaagcggca cttggtaaaa acaaaattgg tacaacagga cgtggtattg
     3241 gcccagctta tgaagataaa gtcgctcgcc gtggtttacg tgtcagcgat ttattcgaca
     3301 aagaacattt tgccgcaaaa ttaaaagata ttttagatta ctacaacttc caattagtca
     3361 attattacaa agttgaagcg gtagattatc aaaaaacctt agatgatgtg tttgcgattg
     3421 cggatgtgat taccggaatg gttgccgatg tgacaacttt acttcacgaa gcacgtgaaa
     3481 acggcgacaa tattttattt gaaggggctc aaggcacgat gttagatatc gaccacggta
     3541 cttacccgtt cgtaaccagc tcgaatacga cagcaggcgg tgtggcaacc ggatcaggct
     3601 acggtcctcg tcatttagat tatgtattag ggattatcaa agcctactgt acccgtgtag
     3661 gtagtggacc gttcaccacc gaactatttg acgatgtagg tgctgaaatt gctcgcaaag
     3721 gtaatgagtt cggagcggta accggtcgcc ctcgccgttg tggctggttt gatgccgtag
     3781 ccgttcgccg agcagtgcaa atcaactcca tttcaggctt ctgtatgact aaactcgatg
     3841 ttttagacgg attcaaagag ctaaaaatct gcgttgccta caaaatgcca aacggaaaaa
     3901 ttgtcgaata tgcccctctt gcagcaaaag actgggaagg tgttgaacca atttatgaaa
     3961 caatgccggg ttggtctgaa aatactttcc gtgtgacaaa acgtgaagat ttaccacaag
     4021 ccgcattaga ttacattaaa cgtatcgaag agctggtcgg tgtgccggta gatattcttt
     4081 ctacaggtcc ggatcgtgtc gaaacgatga ttttgcgtga tccttttgca gcttaataaa
     4141 agataaaata aagccattta gtgagataga gctagatggc ttttttgttt gaaccagtaa
     4201 actttacttt ggcacaattt tgtcacaata cagaaaatag ttctttagcg tgagtttttg
     4261 aggctgtgta agtatttgaa tttaaaagaa tttaatggca cgccctaaag gattcgaacc
     4321 tttgacccac gccttagaag ggcgataggt attttataaa aggcgtttat tttaactaat
     4381 aaaaacaaag acttatagag ataacccctc tatattacct accattattt cacatctttt
     4441 tacattaaat aacaccattt acggtacatt tatggaacac gttggtgatg agctagtctt
     4501 caaaagttac taaattaaac tacaatctgc cccgtctctt ttaagtactt atagatacgc
     4561 cctaacataa tctgcgttgg cgttttgcca atatcctctt ttgttagcgg tgcatcagta
     4621 atctccttgg cggactgtgc atcaatgtat ttgtactcac ttgtgatgat tacaaagttt
     4681 gttacttctc catcggtatc atcgcctgta ccgaccacat atttagcatt gagcgagccg
     4741 tc
//
LOCUS       DANLUQ010000165         4608 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.166, whole genome shotgun sequence.
ACCESSION   DANLUQ010000165 DANLUQ010000000
VERSION     DANLUQ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4608
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.166"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(211..1299)
                     /locus_tag="PXB27_002271"
     CDS             complement(211..1299)
                     /locus_tag="PXB27_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL5347013.1"
                     /translation="MGIMSKSISIFNHKGGIAKTTTAFNVGWSLANQGYQVLLVDLDS
                     QCNLTGLVLGYDQCKEDSDLALFYNNRHHLTMEGIVEALINGQSPDNYLSGNVGQITQ
                     TLNPNLFLLPGHLNVSELDSQISVSLKIAAGVPATRNIPGNLPTLIKKVAEQNNIDYI
                     LYDLSPNVGGLNEVILMSSDYFIVPTSPDFFCWQAVGSLAKNIRKWHNELKAFKELNG
                     FNQSSYAIKNKPQFLGTIQQRYRPRKGQPAKSFEKWIERIRNEVITNFVPVLKTIDCA
                     IDDTAIYAALKSTNSDLEPFDLAHIADFNSLIAISQELRVPVFSLTKEQIKNSGQFGH
                     ALNTMDESKENFDQEFQSLAERIIQLTN"
     gene            complement(1364..1795)
                     /locus_tag="PXB27_002272"
     CDS             complement(1364..1795)
                     /locus_tag="PXB27_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347014.1"
                     /translation="MKKMAIVALSAFFSMNAFANNNVVYSCTTTDNQTLKVTKEGGNY
                     VYSHGNTTFKNPVKEALKNPASEIAGGSQFTTISLELRNAGKSYIVGHIEADPKSPFE
                     ASVQIQDIKTGNDITSFECRSDKPIRHNFDRKLMRKSGFAA"
     gene            complement(1799..3901)
                     /locus_tag="PXB27_002273"
     CDS             complement(1799..3901)
                     /locus_tag="PXB27_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HDL5347015.1"
                     /translation="MKKAKSLKFLTACIMLTTMSGYANSNTVNVGNSISNFIENTTGY
                     RTGNLGVSGNNALTEYYPVLSDYMHYSIGGGSAISAPASRGRPNQYGIGIGWKSNLTC
                     GNFDIKLSIKNQLNGITEGFKDLYGNIIESATGAVASLPAMIIQRANPQLYDILSNGL
                     YQGKLDFNNFKTSCEAMSNQLADLAMDSKLAKMAGLHRAAEIAETEPDAMAAKKKMDK
                     KDAEKGIPWVGGEQRGGKNQKPMAIVADIVGAGYNLALGRGPLEKGDIPIASCDGMLC
                     TEWKNPSEAAAYASEVIGETFLDACETCEQSSSGAKAGKGIAHFIEQTTIEKATKLEA
                     ALNHLDSLTPEQLNSLSTSTVSVTKGVLEAIAASPDAALLGARLSQELAISKEMEKLL
                     LIRRAMLAGMREPNVIKSSLVEEAERNLKLLDREIEQVKLEMELQRSINGNTALAAIT
                     QQMNEQALAIDDNAMGDGNRRFANLSNQAGANNLNTSGIANSAQNAVGDRYIAIPKSD
                     GSGMSDFYGAYKASPTNIVSNVNVGSYTQIAQMNGNANEQATALIKQFEGYLSHGKWD
                     VNANRAGYGSDTYTTADGMVHRVQKGTYVSREDAERDLARRMPQFMNVARGNVGADVW
                     AKLTPQAQAALTSVSYNYGSLSKLESVQKAAAVSAQSGDMTALANSIRNLQGHNNGVN
                     ARRRNQEADYIINAPKGN"
     gene            complement(3928..4206)
                     /locus_tag="PXB27_002274"
     CDS             complement(3928..4206)
                     /locus_tag="PXB27_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL5347016.1"
                     /translation="MQAKTFYYLPLLLLSAMASASPANKPSDVTVKVFTSESYPKVFP
                     HSGDWATHSDSFSNTENYSWALWRPYSCCKKQGQVFLGHVDWKSTHTH"
BASE COUNT         1295 a          901 c          884 g         1528 t
ORIGIN      
        1 atactatatt gcagtgtctg cattttgctg aagagaaggg agctataccg cctatacctg
       61 aagattggcg gtatgaaatg acggggcgta gtatggggat tgagcagatt ccaaatcaga
      121 aattatagct gggcatttac aaaatacaca aaagaaagta tggtgtggct aaattttagc
      181 taaccatact tttttattat gaataattgt ttaattagtc agttgtataa ttcgttctgc
      241 taatgattga aattcttggt caaaattttc tttactttca tccattgtat tcaaagcatg
      301 tccaaattga cctgagtttt taatttgctc ttttgttaag gaaaatacgg gaactcgtag
      361 ctcttggcta atagcaatca acgaattaaa gtcggctatg tgagccaaat caaatggctc
      421 taagtcagaa ttcgttgatt taagagcagc ataaatagct gtatcatcaa tagcacaatc
      481 aatagttttt aataccggca caaaattcgt aatgacttca tttctaattc gctcaatcca
      541 tttttcaaat gatttggccg gttgcccttt tcttggtcga taacgttgct gaatagtacc
      601 taaaaattgt ggcttatttt taattgcata agatgattgg ttaaaaccat taagttcttt
      661 aaatgctttt aactcattat gccatttgcg aatattttta gcgagcgaac caactgcttg
      721 ccagcagaaa aaatcaggtg atgtaggtac aataaagtaa tcactagaca ttaggataac
      781 ttcatttaat ccacccacat ttggactaag atcatagagt atgtaatcaa tattgttttg
      841 ttcagctact ttcttaatta aagtcggaag attgccgggt atatttcttg ttgctggaac
      901 accggcagca atttttaatg atacactaat ttgcgaatca agctcggaaa cattaaggtg
      961 accaggtaac aagaacagat ttggattcag ggtttgagtg atttggccaa catttccact
     1021 aaggtagtta tcagggcttt gtccgtttat cagtgcttca acaatacctt ccatagtcaa
     1081 atgatgtcta ttattgtaaa aaagcgctaa atcactatct tctttgcatt gatcgtagcc
     1141 aagtaccaga ccggttaggt tacattgtga atccaaatcg actaacaata cttgatagcc
     1201 ttgatttgct aaactccaac caacattaaa tgcagttgta gttttagcaa tacctccttt
     1261 atgattaaaa atactgatag acttactcat aatacccata attctatgaa ataattaacc
     1321 caccgaactc atttgagaat ggtgggttaa tgctttgctt tagctaggct gcaaatccag
     1381 attttctcat cagtttgcga tcaaagttgt ggcgaatagg cttatcggaa cgacattcaa
     1441 atgatgtaat atcatttcct gttttaatat cctgaatctg aacacttgct tcaaaaggtg
     1501 atttagggtc tgcttctata tgacctacaa tataagattt tcctgcattt cttaattcga
     1561 gcgagattgt tgtgaattgt gaaccacctg caatctccga tgccggattt ttgagagctt
     1621 cttttacagg atttttgaat gtggtattac cgtgagagta cacatagttt cccccctctt
     1681 tggtaacttt taacgtttgg ttatctgtag ttgtgcaaga atacactaca ttgttatttg
     1741 caaaagcatt cattgagaaa aatgctgata aagctacaat tgccattttt ttcataggtt
     1801 agtttccttt tggtgcgttg ataatgtaat cggcttcctg gttacgtctt cgagcattaa
     1861 caccattatt atgcccttgt aaatttcgga ttgagttagc taaagccgtc atatcacctg
     1921 attgtgcgga gacggcagcc gctttttgaa cactttctag tttagataat gacccatagt
     1981 tgtatgatac cgacgttaat gctgcttgtg cttgaggagt caatttagcc caaacgtcgg
     2041 caccaacatt accccttgca acattcataa attgaggcat tcttcgtgct aaatcacgtt
     2101 cagcatcttc ccggcttaca taagtgcctt tttgaacccg atgaaccata ccatctgcag
     2161 tagtataggt gtcagagcca tatccggcac ggttagcatt tacatcccat ttcccgtggc
     2221 ttaaatatcc ttcaaactgt ttgataagag ccgttgcttg ttcattcgca ttgccgttca
     2281 tttgggcaat ttgggtatag ctccctacat tcacgttact gacaatgttt gttggggagg
     2341 ctttatatgc tccataaaaa tcactcatgc ctgagccgtc tgatttgggg atagcaatat
     2401 atctatcgcc aacggcattt tgtgcagaat tagcaatgcc actggtgttg agattatttg
     2461 ctccggcttg attgcttaaa ttggcaaagc gacggtttcc atcacccatc gcattatcat
     2521 caatagcaag agcttgttcg ttcatctgtt gagtaattgc agctaatgct gtattaccgt
     2581 tgattgaacg ttgcagttcc atttcgagtt taacttgctc aatttctctg tcaagtaatt
     2641 tgaggtttct ttctgcttct tctactaagg aagattttat aacatttggc tcacgcatac
     2701 cggctaacat tgctcggcga atcagtagca atttttccat ttctttagaa atagctaatt
     2761 cttgcgataa ccttgcaccc agcaaggctg catccggcga agcagcaatg gcttctaata
     2821 cgccttttgt tacactgacc gttgatgtac tcagtgaatt gagttgttcc ggcgttaaac
     2881 tatctaagtg attcagtgcc gcttctagct ttgtggcttt ttcaatagtt gtttgttcaa
     2941 taaaatgggc gattcctttc ccggctttag ctccacttga actttgttca caagtttcac
     3001 aggcatcaag gaatgtttca ccaatcactt cacttgcata ggcagcagcc tcgcttggat
     3061 ttttccattc cgtacaaagc ataccatcac agcttgcaat aggtatatct cctttttcta
     3121 atggtcctcg tcctaaagcg aggttatatc cggctccaac aatgtcagca acgatagcca
     3181 ttggtttttg atttttacca ccacgttgtt cgccacctac ccaagggatg cctttttcag
     3241 catctttttt atccattttc ttcttggctg ccattgcatc aggctctgtt tcagcaattt
     3301 cagcggctcg gtgtaatccg gccatctttg ctaatttact gtccattgct aaatcagcaa
     3361 gttggttaga catggcttcg caactggttt tgaagttgtt gaagtcaagt tttccttgat
     3421 atagaccatt agaaagaatg tcatacagtt gaggatttgc tcgttggata atcatcgccg
     3481 ggaggctggc aactgcaccg gttgctgact caataatatt gccataaaga tctttaaaac
     3541 cttcagtaat accattaagc tggtttttaa tggaaagttt aatatcaaag ttgccacaag
     3601 ttaaattcga tttccagcca atgccaatcc catattgatt tggtctgcca cgggaagctg
     3661 gtgcggaaat ggcactacct cctccaatgg agtagtgcat ataatcagaa aggacagggt
     3721 agtattccgt gagagcgtta ttaccagaaa cgcctagatt accagtccta tagcctgttg
     3781 tattttcaat aaaattacta atgctattgc caacattaac ggtattacta tttgcgtatc
     3841 cgctcatggt tgtgagcatt atacaagcgg ttagaaattt gagacttttt gcttttttca
     3901 tttatttatc ctaatagtgc tgataattta atgagtatgt gtacttttcc agtccacatg
     3961 acctaaaaaa acttgccctt gttttttaca gcaagaatag ggcctccata atgcccagct
     4021 gtaattctca gtattggaga aactatctga atgggtagcc caatcaccac tgtgcggaaa
     4081 gactttaggg taggattcgg aagtaaatac cttaactgtt acatctgagg gtttattagc
     4141 aggactagca cttgccatag ctgaaagcag tagtagtggt aaatagtaaa atgtcttagc
     4201 ttgcataaat gctccacaaa tagagtaatt cacgtttacc ttaaaagatg atttggttag
     4261 aagaaatcgg aaattgtttt ttggttttgg agagtttttg ttagtatata agttaatgta
     4321 acaagggaga gtggatgtat gagcaataga atacctaaag cctgttatgc ttacccaaaa
     4381 tcgccgaaca acccgtttaa tagattgtcg gttatcaatc tataccagcc gaaatgtctc
     4441 ccagcattct gaaagggaac tctgtaaaat tacaggaatg ccacctaaac aataaatgga
     4501 taatgccagc cactttatcc gttgtatttt gtcacatgcg tgattgcaat gtcataatat
     4561 tacccgactt ggggatatta tgacattgca atcattgctc acttttct
//
LOCUS       DANLUQ010000166         4524 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.167, whole genome shotgun sequence.
ACCESSION   DANLUQ010000166 DANLUQ010000000
VERSION     DANLUQ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4524
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.167"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(93..803)
                     /locus_tag="PXB27_002275"
     CDS             complement(93..803)
                     /locus_tag="PXB27_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347017.1"
                     /translation="MWKKIRIDFTDTVKCQFCSRMITSGKAIVVQNELGNISFSGPSC
                     AQNKNGQNVVNPKEKIVDITKGCLLHEPTTNDKTDKQVDVEQDNFTSIHDDEVHNYLD
                     NNAVNAYLTLRFEKLSHLTSIIKSQKLNEIYHNYELTGQISQKDESYIRVIMYGEAYP
                     LYTYQNLQAVYAAEYWLQSFIQHNPENDLNFIQDTLNQLRRKLTLTEKQIMGINNWFK
                     NSDGKMVKLKTNAFVRGK"
     gene            1280..1798
                     /locus_tag="PXB27_002276"
     CDS             1280..1798
                     /locus_tag="PXB27_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemophilus-specific protein"
                     /protein_id="HDL5347018.1"
                     /translation="MRYIKYSLFLVSTMLISGCAELSAINDKVGDWAGQMNQTLYGSK
                     DGSVEQHNGRQVYTKTRNGKYVYQDSGTLQLKRNIDDVFLKVRREFGFRPTNADSDSR
                     KVYHTVQGTFYHVSGYFESDVDTDGVHLGENYLEVIIEKGSKNQVNVEWKATGSARWL
                     DSAKQRLLNTLK"
     gene            1958..4009
                     /locus_tag="PXB27_002277"
     CDS             1958..4009
                     /locus_tag="PXB27_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL5347019.1"
                     /translation="MKLFICEKPSQARDLAGVLGATSKGEGLLATADKSIIVTWGFGH
                     LVEQYQPEDYDEAWKRWAFETLPIIPAQWKMTPKKESKKQYSVVIGLIKKATSVVIAT
                     DADREGEMIARELLDLAGYRGQIQRCWLSALDDASIRKALQTLKSGKETEALYYAGMG
                     RSRSDWLIGMNFSRLFTLMAQAKGYSGKPLSVGRVQSPTLTLVVNRDREIANFVPKSH
                     YALAVQLTTTSGETFVAGLAVPEQYLDESGLCLDSRVIQQAEAQIKQTGLAKVVTVET
                     KREKKSPPLLYALSDLQGECNRLFGFGAQQVLDIAQSLYEEHKITTYPRTDCGYLPES
                     QFSDVPNVIRSLVASDSGLQTLLPKLNLRQKSRAWDDKKITAHHGIIPTIKKADLSKL
                     SEEEMKVYDLIRRRYLAQFLPHLEMDKTVATLQSSGHTLVARGNVIVAQGWRILFGKN
                     VDEETGNDDEKQGLPVLTKNQQCNVTHSEVRTLQTKPPAHYTEGTLLDAMKNAARFVT
                     DPRLKQRLRETEGLGTEATRAGLIQGLIDKGFLLKKKKSLVASAEAVALIDSLPDLLK
                     NPGLTALWEQALNQIAEGSMTLDAFMQKQEAFVKQLIGSCMQQGMSLGNIEIRKCSEC
                     GKPMRKINHAKGTFWGCTGYPDCQHKEADKKVKSASNKSAKKSSNLNVLDQLNKLRSQ
                     L"
     gene            complement(4070..4207)
                     /locus_tag="PXB27_002278"
     CDS             complement(4070..4207)
                     /locus_tag="PXB27_002278"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347020.1"
                     /translation="MTLQSRFRQLQQTNVAGIIHLMFSWHSHKVEFPLRMLGDILAGV
                     D"
BASE COUNT         1423 a          862 c          890 g         1349 t
ORIGIN      
        1 aataagcaag caaataatag gaaagagtct tttaaaacca gtcttacgtc gggcctacgt
       61 aacaaaataa gtggccacca caattatgtt aactatttcc ctcttacaaa agcatttgtc
      121 tttaatttca ccatttttcc atcagaattc ttaaaccaat tatttatgcc cataatctgt
      181 ttttcagtta atgttagctt tcttctgagc tgatttaatg tgtcttgtat aaaatttaag
      241 tcattctctg gattatgctg aataaatgat tgaagccaat attcagcagc ataaacagct
      301 tgtaagtttt ggtatgtata caaaggatat gcctcaccat acataatcac tcttatgtaa
      361 ctttcatctt tttgagatat ttgccctgta agttcatagt tatgatagat ttcatttaac
      421 ttttgagatt ttataatcga ggttaaatgc gatagttttt caaatcttaa ggtcaggtat
      481 gcattaacag cattattgtc caaataatta tgtacttcgt catcgtgtat ggatgtgaaa
      541 ttatcttgct caacatctac ttgcttatcc gttttatcat ttgttgttgg ctcatgaagt
      601 aaacatccct ttgtgatatc aactattttc tctttaggat tgactacatt ctgaccgttc
      661 ttattttgag cacaagacgg tcctgaaaaa gagatattgc caagttcatt ttgaacaaca
      721 atagctttgc ctgatgtaat cattctactg caaaattgac atttcactgt atcagtaaaa
      781 tcaatcctaa tttttttcca cataacatat ctctccatat agtgagtaat aaaaatactt
      841 cttaactctc ttcattgatg aaataattgc aaaaataact ttgatagagg ataaatatat
      901 gaatttccct ccaactcgat ggaaaatatt acaccagtat caaactgagc tagagctctg
      961 ggttgaacaa gccacagaag agcatatcaa gcaatacttg actgaaattc acggatgtgt
     1021 atacccagat gactgggaaa atgtgcgtat atcccatatg tcatctcaaa ctcatatttt
     1081 ccatagctgt gaacgttgcc tagaacttat ctctgcgata ttaacaatct cacaactaag
     1141 cagacccgat ctaaatggtc tactgtgtga cagaaagcta taatattttt tctctttgta
     1201 agtaaaagag ttttaagtag attttaaccg cacttttgca aaaaatgaac gcagataacc
     1261 cgcttgtaag gagaattcta tgcgttacat caaatatagt ttatttcttg tttctacaat
     1321 gctaatttca ggctgtgctg aactctcagc aattaacgat aaggttgggg attgggccgg
     1381 gcaaatgaat cagacgctct atggctctaa agacggctct gttgaacagc ataatgggcg
     1441 acaagtttat actaaaaccc gaaatggtaa gtacgtttat caagattccg gcacattgca
     1501 gttgaagcgg aatattgatg atgtattcct gaaagttcgt cgggaatttg gcttccgccc
     1561 aacgaatgct gatagtgact cccgtaaagt ttatcatacg gtacaaggta ctttttacca
     1621 tgtttccggc tattttgaat ctgatgtaga tactgatggg gtacacttag gtgaaaacta
     1681 cttggaagtt atcattgaaa aaggcagtaa aaatcaggtg aatgttgaat ggaaagcaac
     1741 tggtagtgct agatggctcg atagtgcaaa acagcgttta cttaatacat taaaataata
     1801 actcatattg ctcctagaac ctcttcccag tggaaacact gggatttttt atgtccttat
     1861 ttctcaaatt attacatcct acgagacaat aagtcgttga atgctattta atcagcacat
     1921 tactatttgc ctatctgtat tcattttagg aaataatatg aaattattta tttgtgaaaa
     1981 gccatctcaa gctcgagatc ttgccggcgt attgggtgct acttcaaaag gtgaaggctt
     2041 gctggcgact gcggataaat ctattattgt cacttggggg ttcgggcatc tcgttgagca
     2101 atatcagccg gaagattatg atgaagcgtg gaaacgctgg gcatttgaaa cattacccat
     2161 tattcctgct cagtggaaaa tgacaccgaa aaaagagtct aagaagcaat attctgttgt
     2221 aatagggctt atcaaaaaag caacctccgt ggtcattgcg accgatgctg atcgagaagg
     2281 tgagatgatt gctcgtgagc tgttagattt agctggatac cgaggtcaaa tccaacgttg
     2341 ctggctatca gctctggatg atgccagtat tcgaaaggct cttcaaacac tcaaatcagg
     2401 taaagaaacc gaggctcttt actacgccgg tatgggacgt agccgctcgg actggttgat
     2461 tggaatgaac ttctcccgtt tatttacctt aatggctcaa gcaaaaggct attccggtaa
     2521 gccattgagc gttggtcgag tacaaagtcc aacattaact cttgtggtta atcgagatag
     2581 agaaattgct aatttcgtgc ctaaatctca ttatgcgtta gcggttcagc tgacaacaac
     2641 ctctggtgaa actttcgttg ccgggcttgc agtacctgaa cagtatttgg atgagtccgg
     2701 actttgcctt gatagtcgtg tcatacaaca agccgaagct caaattaaac aaacaggctt
     2761 agccaaagtg gtgacggttg aaactaaacg agagaaaaaa tctccaccgt tactctacgc
     2821 attaagtgat ttacaaggcg aatgtaaccg cttgtttggc tttggggctc agcaggtgtt
     2881 ggatatcgca caatcccttt acgaagagca taaaatcacg acttatcccc gtacagactg
     2941 tggttatctg ccagaatcac aattttctga tgttcctaac gtaatccgct cattagttgc
     3001 ctctgattca gggctacaaa ctctcttgcc taaattaaat ttgcgacaaa aaagtcgggc
     3061 gtgggacgat aaaaaaatta ccgctcacca cggtattatc ccaaccatta aaaaagctga
     3121 tttaagtaaa ttgagtgagg aggaaatgaa ggtctatgat ctcattcgcc gccgatatct
     3181 tgctcaattc ttaccgcact tggaaatgga taaaaccgtt gccactctgc aatctagtgg
     3241 gcacactctg gttgctcggg gaaatgtcat cgttgctcaa ggctggcgta tcttgtttgg
     3301 caaaaatgtg gatgaggaaa ctggtaatga tgatgagaaa caaggattac ctgtattgac
     3361 taaaaatcag caatgcaatg tcactcattc tgaagtccga acattgcaaa ctaaacctcc
     3421 cgcacactat acagagggga cattactcga tgcaatgaaa aatgccgctc gctttgtgac
     3481 tgacccaaga ctcaaacaac gcttacgtga aacggaaggt ttaggcacag aagcaacccg
     3541 tgccggatta attcaaggcc taattgacaa aggatttctc cttaagaaga aaaaatcact
     3601 ggttgcctca gcagaagccg ttgccttaat tgatagtttg ccggacttac tgaaaaatcc
     3661 cggtttaacg gcattatggg aacaagccct caatcagatt gccgaaggta gtatgacact
     3721 tgatgcattt atgcaaaagc aagaagcatt tgtaaaacaa cttatcggca gttgtatgca
     3781 acagggaatg tcacttggga atattgaaat ccgaaaatgt tcagaatgtg gtaagccaat
     3841 gagaaaaatc aatcacgcta agggaacatt ctggggatgt actggttatc cagattgcca
     3901 acataaagag gctgataaaa aggtaaaatc agcttcaaat aaatctgcta agaaaagtag
     3961 taatttaaat gtactcgatc aactgaataa attaagaagc cagttataaa ttcaagtaga
     4021 ttatggtatt atcaaggaac aaagaacccg ttttgtagat tgtcggttat caatctacac
     4081 cagccaaaat gtctcccagc attctgagag ggaactccac tttgtgggaa tgccaactaa
     4141 acattaaatg gataatgcca gccacattag tctgttgtaa ttgtctaaat cgtgattgca
     4201 acgtcataat attacccgat tggggggatt atgacgttgc aattactgct aactattctg
     4261 agcctgagcc cacaggtgaa taaagggtgc cgtttgtgtc ctcgatgatg ccacagatat
     4321 gccgattaaa gaccacaagg taatcgggta aattacgaac gaaaatatca acattgtcac
     4381 gaaggtgctg ctattttcaa tatagtaact tatattggtc taacttaaat ttcatttatt
     4441 cttcaaaagt acaacaccgt tggtaagtga tttatcaacg gtgttgtcat ttccaattct
     4501 ctaaaatcaa aattcaattc tcaa
//
LOCUS       DANLUQ010000167         4511 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.168, whole genome shotgun sequence.
ACCESSION   DANLUQ010000167 DANLUQ010000000
VERSION     DANLUQ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4511
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.168"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..608
                     /locus_tag="PXB27_002279"
     CDS             <1..608
                     /locus_tag="PXB27_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347021.1"
                     /translation="DSSIELEGKESEEEFELEHIFKKYRFDSFSTYTGLISIYSSTFG
                     SVNIESEIIKNGICLNFARQLISAKHPETKKRISLHLKILDFDKGRNTYLAPADLFIL
                     DEHLNEFILGNGGDEIEKISKNNKTQFSSTIRENQINFIRALLYMDYGIETPQEVKNA
                     INSGELGRKIEKFRRENPNIENDKNFKFPTSVTLSNWYSKA"
     gene            686..937
                     /locus_tag="PXB27_002280"
     CDS             686..937
                     /locus_tag="PXB27_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5347022.1"
                     /translation="MQLQKTISTFTQPNHRGIYVMTYSEQSYLNVKDVCEMLQLNRST
                     IYRQIKQGIFPKPTKIGKSSRWKFADIKAFLEPKPQEAN"
     gene            937..1317
                     /locus_tag="PXB27_002281"
     CDS             937..1317
                     /locus_tag="PXB27_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL5347023.1"
                     /translation="MAGLSIICPFCGNTARIRGSSRPSLLSYQAQLTCSNCGQLKADF
                     VGQLINIKRAVFIECDEANQWDKPENELIKEGKIQGKDNAQRLKELRGSQQDLFAKVP
                     GQLPPEKITPAEKIAKRANLSKTH"
     gene            1628..2689
                     /locus_tag="PXB27_002282"
     CDS             1628..2689
                     /locus_tag="PXB27_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HDL5347024.1"
                     /translation="MKDLSPNQKQYTPFSAIMQKNLMDLLTKSARSPYSFSTVAKSTA
                     GRENPELHNTANSSTPLTRAIFIRSLHTPKERRNKACSSMVACSGQGSPLAVFGSPFV
                     AVFSPRYTLSPCTVRSVADSPLKLTNGLQKMNPSQITGKNRLNSFLFNNNQNDYSLLF
                     TNAKQSTPISAKISKNQDLCLTTLNRSPYASRAFAKSKAECGNSKEHQANNSTPLTRA
                     FFVRSSRTPKECQNKACSSMVACSGQGFALDCLPDVAVFHPVTRYRPTLWKMLAIAPK
                     NQHQELSKMKAFAYSFLCAIRTPETDTYQETVVRIISDSEQNARFQLTADYRLLLPRP
                     VAKIRLNSTAYTVKGGIYA"
     gene            2682..2846
                     /locus_tag="PXB27_002283"
     CDS             2682..2846
                     /locus_tag="PXB27_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347025.1"
                     /translation="MLNYSDIEQIRYFMDQAEEALDNNEEFKAVAELNTAQQFILALL
                     MNYEQGGENE"
     gene            2839..3474
                     /locus_tag="PXB27_002284"
     CDS             2839..3474
                     /locus_tag="PXB27_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347026.1"
                     /translation="MNNTTGQATQRNWQEIIANLQRLSESGESTIEIAKRYEAFSKTA
                     EQIQALVNNNGSEAEKEAFFAPVERFYLGLLETETRLKDAIAKDEENAQKLKEQKAAR
                     EAEQKRWDNLPFYAKDKFSDLICIESRETLEDKFFAISCMFDALKAVPNLLEIGLREE
                     GKDSVIDRLAEMSKLYLLFVDEQFSLLANEIQSGKYGKLDLNGSQNKGDLC"
     gene            3468..3686
                     /locus_tag="PXB27_002285"
     CDS             3468..3686
                     /locus_tag="PXB27_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347027.1"
                     /translation="MLTHRITLPDGTEIEFFAETDKETFAEIQRDLEGLKLPTFAEFV
                     AQERKKHRAKQAVKKARKIAKLARRKNR"
     gene            3828..4313
                     /locus_tag="PXB27_002286"
     CDS             3828..4313
                     /locus_tag="PXB27_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347028.1"
                     /translation="METALQLAQHLTGWTEEENSQAILKNFLDWKETFGEKTREETKI
                     VETLLDWINENEASFIEYPFNQEQRTPPKVAGYRILANNSTKTNEYFFIYPKQFKETL
                     AEFPLLMACTKLHEAGILHKPPRPEKGYEFQYTIAPKITGGSKKRAYKIVPIALEEEN
                     E"
BASE COUNT         1654 a          899 c          838 g         1120 t
ORIGIN      
        1 aagatagctc tattgaatta gaaggcaaag aatcagaaga agaatttgaa ttagaacata
       61 tatttaaaaa gtataggttt gatagtttca gcacttatac tggattaatt tctatttaca
      121 gcagtacttt tggtagtgta aatattgaat ctgaaatcat taaaaatgga atttgtttaa
      181 attttgcaag acaattaata tcagcaaagc acccagaaac aaaaaaaaga ataagtttac
      241 atctaaaaat actggatttt gacaaaggca gaaatactta tttagcacct gcagatttat
      301 ttattcttga tgaacatcta aatgagttca tattaggtaa cggcggagat gaaatagaaa
      361 aaatatcaaa gaataacaaa actcaattca gctcaacaat acgagaaaac caaatcaatt
      421 ttattagagc cttgctttat atggattatg ggatagaaac gcctcaagaa gtaaaaaacg
      481 ccattaattc aggggaacta ggcagaaaaa tagagaaatt taggagagaa aaccccaata
      541 tagagaacga taaaaatttc aaattcccta caagtgtaac ccttagcaac tggtattcaa
      601 aagcctaaaa tattggaaat ttccgaatct actttggaaa tttccaaact tctaaaacct
      661 actcacctca caatctcccc caacaatgca actacaaaag acaataagca cattcacgca
      721 accaaaccac agggggattt atgtaatgac ctattcagaa caaagctatt taaacgtgaa
      781 agatgtatgc gaaatgctac aactcaaccg ctcaactatt tatcgacaaa ttaagcaagg
      841 gattttccct aagccaacta agatcggaaa atcaagccgg tggaaatttg ccgacatcaa
      901 agcattttta gagccaaaac cacaggaggc gaactaatgg cagggctttc tattatttgc
      961 ccgttttgtg gcaataccgc acgaattaga ggcagttctc gccctagcct tttaagttat
     1021 caagcacagc taacttgttc aaattgcgga caattaaaag ccgactttgt agggcagcta
     1081 atcaacatca aacgggccgt atttattgag tgtgatgaag caaatcaatg ggataaaccc
     1141 gaaaatgaac taatcaagga gggaaaaatt caaggcaaag acaatgctca acgcctcaaa
     1201 gaactaagag gctcacagca agatttattt gcaaaggtgc cggggcaact accgcccgag
     1261 aagatcactc cggcggagaa aatcgccaag cgggcaaacc tttcaaaaac gcattaaaca
     1321 gggggataaa tggcaacaaa aaagccccgc aaggcaaacc atacggagct attaaaatcg
     1381 cataatgcga aagatatgca aagaaattaa agcaggcttg acgatttctg aaggtgttag
     1441 attctttcta aagtctttag ggataggttt atttctagca ttgttttttt attttttgcc
     1501 gtatttagca gaatttataa tcgccgtaaa aaaacaacta gcaaaaaaat cggcttactt
     1561 tcaatgataa aagcaagccg aaatgacaat ccaaattttt aactttaaat aaagagtaca
     1621 agaagaaatg aaagatttat ctccaaatca gaaacaatat acgccatttt cggctataat
     1681 gcaaaaaaat ttaatggatt tattgacaaa atccgcccgt tcgccttatt ctttctctac
     1741 cgttgcaaaa tcaacggcag ggcgtgagaa ccctgaacta cataatacgg cgaacagtag
     1801 cacgccttta acccgtgcta tttttattcg tagcttacac acacctaaag aacgccgaaa
     1861 taaggcgtgt tcgtctatgg tagcgtgtag cgggcaaggt tcgcccttgg ctgtttttgg
     1921 ttcgccgttt gtagcagttt tctcaccccg ttacacgcta tcgccctgca ccgtgagaag
     1981 tgtagccgat agtcctttaa aattaacaaa cggactacaa aaaatgaacc ctagccaaat
     2041 tacaggcaaa aaccgcctaa actcttttct atttaacaat aatcaaaatg attattcgct
     2101 attatttact aatgcaaaac aatcaacccc gatttctgct aaaataagca aaaatcaaga
     2161 tttatgcttg acaaccctca atcgttcgcc ttatgctagc cgtgcttttg caaaatcaaa
     2221 agccgagtgt ggaaactcga aagaacatca ggcgaacaat agcacgcctt taactcgtgc
     2281 tttttttgtt cgtagctcac gcacacccaa agaatgccaa aataaggcgt gttcgtctat
     2341 ggtagcgtgt agcgggcaag gtttcgccct tgactgctta cctgatgttg cagttttcca
     2401 ccccgttaca cgctatcgcc ctacattgtg gaaaatgtta gcgatagccc ctaaaaatca
     2461 acatcaggag ctatcaaaaa tgaaagcatt cgcttattca ttcctttgtg ctattcgcac
     2521 acccgaaaca gatacttatc aagaaaccgt agtacgcatt atttcagaca gcgaacaaaa
     2581 cgcccgtttt cagcttacag ccgattatcg cttactactc ccccgccctg ttgcaaaaat
     2641 ccgcctaaat tctaccgctt acacggtgaa aggggggatt tatgcttaat tattccgata
     2701 ttgaacaaat ccgctatttt atggatcaag ccgaagaggc tttagacaat aatgaagagt
     2761 tcaaagccgt agcagaactt aacacagcac aacaatttat tctggcttta ttaatgaact
     2821 acgaacaagg gggtgaaaat gaataataca acaggacaag ctacgcaaag aaactggcaa
     2881 gaaattatcg ctaatttgca gagactcagc gaatccgggg aaagcacaat cgaaatcgct
     2941 aaacgctatg aggcattttc taaaaccgcc gagcaaatcc aagccctagt gaataacaac
     3001 ggcagcgaag ccgaaaaaga ggcgtttttt gcccctgttg agcgttttta tcttggctta
     3061 ctagaaaccg aaacacgctt aaaagatgcc atagccaaag acgaagaaaa cgcccaaaaa
     3121 ttgaaagagc aaaaagcagc tagagaggca gagcaaaaaa gatgggacaa cctacctttc
     3181 tacgccaaag acaaattcag tgatttaatt tgcattgaaa gccgagaaac gcttgaagat
     3241 aaattctttg cgatctcttg tatgttcgat gccctaaaag ccgtacctaa tctattagaa
     3301 ataggcttgc gtgaagaagg caaagacagt gttatagacc gattagccga aatgagcaaa
     3361 ctctatttac tgtttgtaga tgagcaattt tcactactgg caaacgaaat tcaaagcggt
     3421 aaatatggca agttagacct aaacggctca cagaataagg gggatttatg ctaacgcacc
     3481 gcattacact ccctgacggc acagaaattg aattttttgc agaaaccgac aaagaaacat
     3541 ttgcagaaat tcaaagggac ttagagggct tgaaattgcc aacctttgcc gagtttgttg
     3601 cccaagagcg gaaaaaacac cgagcaaaac aagcggtcaa aaaagcccga aaaattgcaa
     3661 aattagcaag acggaaaaac cgctagcacc tatcaaaaag acttattatt gcagaaaatc
     3721 acgctttaat aaaaacgacc tataaagcaa tccgcaagaa atggttaagt ttaactgaaa
     3781 atatgagcgg acaggttcag cgggtagcgt gcgatagatt cgcaataatg gaaaccgcct
     3841 tgcagttagc ccaacattta acaggctgga cagaggaaga gaacagccaa gcgattttaa
     3901 aaaacttcct agactggaaa gaaactttcg gcgaaaaaac aagagaagaa actaaaatag
     3961 tagaaacctt gctagattgg ataaatgaaa atgaggcgag ttttatcgag tacccattca
     4021 accaagagca aagaacaccg cctaaagttg ccggctatag aatacttgcg aataacagca
     4081 ctaaaacaaa tgagtacttt ttcatttacc ctaagcaatt taaagaaacg ctggcagaat
     4141 ttccgctatt gatggcttgc accaagttac acgaggcagg gattttacat aagccaccac
     4201 gcccagaaaa aggctatgaa tttcagtaca caatagcccc taaaataaca ggagggagca
     4261 aaaaaagagc ttataaaatc gtaccgatag cattggaaga agaaaacgag taattacagc
     4321 aaaagcaaga gtttcaattc ttgctttttt tatacttcaa acaaaataaa aataaataca
     4381 aattaaaaat ttatagggaa aatagggaaa aaataagaag aatatagata tatcaaggtt
     4441 ttatagattt ttattttccc tattttatat aaaattttcc ctaaatcttc ctattttttc
     4501 cctatttatg c
//
LOCUS       DANLUQ010000168         4505 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.169, whole genome shotgun sequence.
ACCESSION   DANLUQ010000168 DANLUQ010000000
VERSION     DANLUQ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.169"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..633
                     /locus_tag="PXB27_002287"
     CDS             <1..633
                     /locus_tag="PXB27_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HDL5347029.1"
                     /translation="ENPLAYEDYSLINQKFLKFQWKSNYAYANWLETTANKIKRSKDD
                     SIEWHRKNEKEIPFWVITETWDFGALSKFYSLLNGQHQRKIAKKFNVDENTLKNWLNE
                     INIVRNLSAHHSRIWNRKFSDIAIPKLDDPRWSKFKKMQQYFSNLSLDDKAKERIFGR
                     VAILWFLVSQTSTNYHWLDKFEDLLNDFPNLPNTKVELMGFNREILEKIK"
     gene            complement(697..1659)
                     /gene="hemH"
                     /locus_tag="PXB27_002288"
     CDS             complement(697..1659)
                     /gene="hemH"
                     /locus_tag="PXB27_002288"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HDL5347030.1"
                     /translation="MNQPQQIGILLANLGTPDEPTPSAVKRYLKQFLSDPRVIDLPKF
                     KWQAILNCIILPRRSPKVAKLYQSVWSEEGSPLLAISRQQQRAVQRYFDAKGKNVVVE
                     LGMSYGNPSIESATDNLIKQGVERIIVLPLYPQYSSTTTASVFDAFANGLKAQRKIVP
                     FDFIHSYHNDPLYIQALANSIDVKPDELLLFSFHGIPKRYETEGDFYAKHCQETAELV
                     AEKLGLPREKWYLSFQSRFGDEEWLQPYTDQTLESFPQKGIKKVAVVCPGFSADCLET
                     LEEITEENRENFEHAGGESYRYIPALNASGDHIKALVKLIEAKI"
     gene            1896..2030
                     /gene="rpmH"
                     /locus_tag="PXB27_002289"
     CDS             1896..2030
                     /gene="rpmH"
                     /locus_tag="PXB27_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDL5347031.1"
                     /translation="MKRTFQPSVLKRARTHGFRARMATKNGRQVLARRRAKGRKSLSA
                     "
     gene            2066..2440
                     /gene="rnpA"
                     /locus_tag="PXB27_002290"
     CDS             2066..2440
                     /gene="rnpA"
                     /locus_tag="PXB27_002290"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDL5347032.1"
                     /translation="MKKLNFSRELRLLAPVQFKAVFEEPLRASTPQMTLLARENNLEN
                     PRLGLTVAKKHLKRAHDRNRIKRIVRESFRLKQHELPNFDFVFVAKGGIGKLDNATLF
                     ETLEKLWSRHIRLSKKPAQQNP"
     gene            2389..2712
                     /gene="yidD"
                     /locus_tag="PXB27_002291"
     CDS             2389..2712
                     /gene="yidD"
                     /locus_tag="PXB27_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDL5347033.1"
                     /translation="MVKTHSPEQKAGTTESINQPPRLSLLAKILLLPIYFYRYAISPL
                     IGPRCRFYPTCSTYAVEAIKIHGAIKGGYLAFKRILRCNPLSEGGEDPVPLCCKGNCK
                     HNDKK"
     gene            complement(2817..3158)
                     /gene="erpA"
                     /locus_tag="PXB27_002292"
     CDS             complement(2817..3158)
                     /gene="erpA"
                     /locus_tag="PXB27_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005706127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HDL5347034.1"
                     /translation="MSDIQIPLIFTDAAARKVKSLIEGEENPNLRLRVYITGGGCSGF
                     QYGFTFDDQVNEGDLTIENQSVGLVVDPMSLQYLIGGTVDYTEGLDGSRFVVNNPNAS
                     STCGCGSSFSI"
     gene            3307..4434
                     /locus_tag="PXB27_002293"
     CDS             3307..4434
                     /locus_tag="PXB27_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HDL5347035.1"
                     /translation="MADSVKSHEQSSDEIDLIEIFRALWDKKWWIVITTFITTLLAGI
                     YAFTAKEQWTSKATVIAPKLTELGEYLNVRREYSRILRVDPVDPNALSASLFNNFNRL
                     AQSQDTKNEFFITSNVYKTLAEGKDEQGQRTVLSNLSNENTSFIIPDAKKDPNAIGRT
                     LSFSAETPMEAQDTLGKLVQFINSKAFAVELEDFLVDFQNVLTALQYEKSLIQEDLSV
                     NKSVQLENLNKAYETAQKAGIKEYSKAFNQSDASNIAAALSDTKISLSDSKLADSSYL
                     FMLGEKYLKAQIDVANEKGVVYPPRYYQIDYQLKQLEPLFEKTKTVKTQTFSYQASPD
                     YPVIKDKPKKVIILLIGAFIGLMISCLSIILTHYLLASKFK"
BASE COUNT         1445 a          827 c          845 g         1388 t
ORIGIN      
        1 gaaaatcctt tggcttatga agattattct ctgattaatc aaaagttctt aaaatttcaa
       61 tggaaatcta attatgctta tgcaaattgg ctagaaacta cagctaataa aattaagcgt
      121 agtaaagatg attctattga atggcataga aaaaatgaga aggaaatccc tttttgggta
      181 attactgaaa cctgggattt tggtgcatta tctaagtttt actcattgtt aaatgggcaa
      241 caccaaagga aaattgctaa gaaatttaat gtagatgaga atacgttaaa gaattggttg
      301 aatgaaataa acattgtaag aaacctttct gctcaccatt ctcgtatttg gaacagaaaa
      361 tttagcgata ttgctattcc taagctagat gatcctcgtt ggtcgaaatt taaaaaaatg
      421 cagcagtatt tttcaaactt atcgctggat gacaaagcaa aggagagaat tttcggaagg
      481 gtagctattc tgtggttttt agttagtcaa accagcacta attaccattg gttagataag
      541 tttgaagatt tattaaatga cttccctaat ttaccgaata caaaagtaga actgatggga
      601 tttaacagag aaatattaga gaaaattaaa taaaataagt acattcctat agggtgtaga
      661 taaaaatggg cggatttatt atccgcccaa attatattat attttcgctt cgattaattt
      721 tactaatgcc ttgatatgat cgccactcgc atttaacgct ggaatgtagc gataagattc
      781 tccgccggca tgttcaaagt tctcgcggtt ttcttctgta atttcttcta aggtttccaa
      841 acaatctgct gaaaagcccg ggcaaacgac agccactttt tttatccctt tttgtgggaa
      901 gctttcaagg gtttgatcgg tataaggctg tagccactct tcatcaccaa aacgggactg
      961 gaaagacaaa taccattttt ctcttggcag acctagcttt tcagccacta attctgctgt
     1021 ttcttggcaa tgcttggcgt aaaaatcacc ttccgtttca taacgtttag gaatgccgtg
     1081 aaatgaaaac agtaataact cgtcaggttt aacatcaata gagttagcca acgcttgaat
     1141 ataaagcgga tcgttatgat agctgtgaat aaaatcaaac ggcacgattt tacgctgtgc
     1201 ttttaaaccg ttggcaaacg catcaaacac cgaagcggta gtagtgctac tgtattgcgg
     1261 ataaagcggt agcacaataa tccgctcaac cccctgcttg atgaggttat cggtcgcact
     1321 ttcaatactt ggattgccgt aagacattcc caattccacc accacatttt tgcctttggc
     1381 atcaaaatag cgttgtaccg ctcgttgttg ttgccgagaa atcgccaata acggtgagcc
     1441 ttcttcgctc catactgatt gatataattt tgccactttt ggcgaacgtc ttggcaaaat
     1501 gatgcagttt aggattgctt gccatttaaa ttttggtaga tcgatcacac gaggatcgct
     1561 taaaaattgc tttaaataac gcttcaccgc acttggcgtg ggttcatctg gcgtacctaa
     1621 attagccagc aaaataccta tttgttgggg ttgattcatt gttctatttc tcatcaatag
     1681 ttaatatttt ttcgattatg ccacaaacca atataaattt ctgcgattat aacaagcggt
     1741 cgtatttttc aaaaattttg caaacaaccc gagttgtgct tggattgttt cttgcttttt
     1801 attttaattg cctgtagaat tgcaactctt ttttatggtc cttgtttggc agaaaccatt
     1861 ttatgccaac tttttaaatt ttaggtagat aagcaatgaa acgtacattt caaccatcag
     1921 tattaaaacg tgctcgtact cacggtttcc gtgctcgtat ggctactaaa aacggtcgtc
     1981 aagtgttagc gcgtcgtcgt gctaaaggtc gtaaatcttt atctgcataa ttgcagaacc
     2041 agccaaaaca cctttctgat taagtgtgaa gaagctaaat ttctctcggg agctacgttt
     2101 gttagctccc gttcaattta aggcggtgtt tgaagagcca cttcgagcca gcacacccca
     2161 aatgactctt cttgctagag agaataatct agaaaatcct cgtctaggct taactgttgc
     2221 caaaaaacat ttaaaacggg ctcacgaccg taatcgcatt aagcggatag tgcgtgaaag
     2281 ttttcgcctc aagcaacacg aattgccgaa tttcgatttt gtatttgtgg caaaaggcgg
     2341 cattggcaaa ttagacaacg caacgctgtt tgaaacattg gaaaaactat ggtcaagaca
     2401 cattcgcctg agcaaaaagc cggcacaaca gaatccataa atcagccacc tcgtctcagc
     2461 ctattagcca aaattctatt gctaccgatt tatttctatc gctatgctat cagcccattg
     2521 attggcccac gttgccgatt ttacccaacc tgctccacct atgcggtcga agcgattaaa
     2581 atccacggag caattaaagg tgggtattta gcgtttaagc gtattctgcg ttgcaaccct
     2641 ttgagtgagg gaggcgaaga tcctgtccca ctttgttgta aaggcaactg caaacataat
     2701 gataaaaaat agcctaaact caataagtta ggctattttg tttaatacaa acggtcattt
     2761 ttagtaaaaa atttacttgt gagactgata ttgaataagg caataattcg agtgaattaa
     2821 atgctaaagg aagaaccaca accacaggtt gagcttgcat ttggattatt cacgacaaaa
     2881 cgtgagccgt ccagcccttc ggtataatcc accgtgccac caattaaata ttgtaggctc
     2941 atcgggtcaa ccaccaagcc aacgctttga ttctcaatgg taaggtctcc ctcattgact
     3001 tgatcatcaa aggtgaaccc atactggaat ccactacagc ccccacccgt aatatacaca
     3061 cgcaagcgaa gattcgggtt ctcttcgccc tcaattaagc tttttacttt acgagccgcc
     3121 gcatcagtaa agattaaagg aatttgaata tcgctcattt tgttttagat caaaatttta
     3181 ctgtgattgg atattatcta ataattacat aggaagtgca atcttaaaaa agagtaaatg
     3241 aatgcagtgg aaataagaaa attatgtata atgctgtaca gaaacatgca aattttagag
     3301 gaaactatgg cagattcagt aaaaagtcat gagcaaagct cggatgagat tgatttaatt
     3361 gagatttttc gtgctctatg ggataaaaaa tggtggattg ttataacaac attcattacc
     3421 acattacttg ctggtattta tgcatttact gcaaaagagc aatggacgtc taaggcaaca
     3481 gttattgccc ctaaattaac agagttaggt gagtatttaa atgttcgcag agaatatagt
     3541 cggattttgc gtgtagatcc tgttgatcca aatgcattgt ctgcgagttt gttcaacaat
     3601 tttaaccgat tagcacaatc tcaagatact aaaaatgaat tttttatcac ttccaatgtg
     3661 tataagacgt tagctgaagg aaaagatgag caggggcaac gcacagtttt atccaatttg
     3721 agtaatgaaa ataccagctt tattattcct gatgcaaaga aagatcctaa tgcaataggt
     3781 cgtacactat ctttttctgc agagacacct atggaggcgc aggacaccct tggtaagttg
     3841 gttcaattca ttaatagcaa agcatttgct gttgaattag aagattttct agtcgatttc
     3901 caaaatgtgc ttactgcatt acaatatgaa aaatcattaa ttcaggaaga tttatctgtg
     3961 aataagtcgg ttcagttgga aaacttaaat aaagcctatg aaactgcaca aaaagcaggt
     4021 attaaagaat attcaaaagc atttaatcaa tcagatgcat caaatattgc agcagcttta
     4081 agtgatacta aaatctccct ttctgattca aaattagcag atagttcata tttatttatg
     4141 ttaggggaaa aatacctaaa ggctcaaatt gatgtggcta atgagaaagg tgttgtttat
     4201 ccaccacgtt attatcaaat tgattaccaa cttaaacagc tagagccgtt atttgaaaaa
     4261 acaaagacag ttaaaacaca aacttttagt taccaagctt cgccggatta tcctgtaata
     4321 aaagataaac ctaaaaaggt aattatttta ttaataggag cttttattgg tttgatgatt
     4381 tcttgtttgt caattatcct aactcattat ctgcttgcga gtaaatttaa atgaatttga
     4441 ttatttgtta cactccatta caagtgaaaa tagctgaatc tattgtaaaa aagtactcag
     4501 gagaa
//
LOCUS       DANLUQ010000169         4495 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.170, whole genome shotgun sequence.
ACCESSION   DANLUQ010000169 DANLUQ010000000
VERSION     DANLUQ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4495
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.170"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            60..953
                     /locus_tag="PXB27_002294"
     CDS             60..953
                     /locus_tag="PXB27_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HDL5347036.1"
                     /translation="MNKKLPPLNALKAFESAARHLNFTKAAEDLFVTQAAVSHQIKLL
                     EDFLGASLFHRRNRLLELTELGAQYFYEVQPLLEQIALATDKIRQQSSRQILTISVPQ
                     TFGMHWLVPRLADFHEKYPEVEVRIKGVDQDEGLLGKETDVAIYYGKGDWQGLESIRL
                     TESRIVILASPEYLSQNTIHLPADLAGKHLLHSSTHNKWKRMVEHLHIEDIVDAETGS
                     IFSHTFMVLQAAMHQQGIAIANQLLAQHEIEKGTLIEPFPTTLFDEKSFYVVYPSQSA
                     QVQKVQFFVEWIQQKMKECCA"
     gene            1029..1712
                     /gene="yjjG"
                     /locus_tag="PXB27_002295"
     CDS             1029..1712
                     /gene="yjjG"
                     /locus_tag="PXB27_002295"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HDL5347037.1"
                     /translation="MKYQWVLFDADETLFSFNSYLGLKAMLARYQIDFSEQDYQDFQA
                     VNKPLWVAYQNNEITAQDIQTRRFVKLSAQTGVDPLQLNQELMAEMAFVSQPLEGVMD
                     MLNALYGKVKMGIITNGFTDLQQKRLDNTQTSHFFDIVVISEQIGAAKPDRQVFDYAF
                     ALMDEFDRTKVLMVGDTLASDILGGNNAGIDTCWLNLLSKENDTDIKPTYEVHTMQQI
                     IAIVEGKSL"
     gene            1709..2692
                     /gene="cysB"
                     /locus_tag="PXB27_002296"
     CDS             1709..2692
                     /gene="cysB"
                     /locus_tag="PXB27_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HDL5347038.1"
                     /translation="MKLQQLRYIVEIVNQNLNITEAAEVLYTSQPGISKQVRLLESEL
                     GINIFERNGKHVKGLTPAGEKIVAIARELLVKAQSIKSVAEEQTRPNKGILRIATTNT
                     QARYMLPSVIEKFKARYPEVSLHLHQGSPNQIYDALVAGEVDLAITTEAQYLFDEAVL
                     LPCYWWNRSVIVKPEHPLAAFVNQSKNLTVEQLGRYDLITYTFGFTGQSDLDHAFNKE
                     GILPNIVFTATDADVIKTYVRLGLGVGIMASMAHTETDKDLVAINASHLFQPSMTQIA
                     FKRGTFLRNYMYDFINYFSPHLTRVQVEKAEQLRDNNAISRLFESISLEEK"
     gene            2693..3337
                     /locus_tag="PXB27_002297"
     CDS             2693..3337
                     /locus_tag="PXB27_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347039.1"
                     /translation="MRIWQKLASVIVVTLGLISNASAENFKLKYSSNYLMPAYINFNN
                     GGGQYNIQAKINVPLYNIVFSAKGTEKNEQFHMLSYRDTRNGKTYAMAEIDSKTVKYG
                     KVKEPLKEEALTMPTYDLFTTAFQLSYYDKLPSSFQTTNGKKLYPTPNVQLRKAQKEV
                     KVGGKTYQEITYKFRTSDKKDITVKKFEGEKFPRYIAYSRDGDNYELTFDSFVK"
     gene            complement(3410..4459)
                     /gene="ansB"
                     /locus_tag="PXB27_002298"
     CDS             complement(3410..4459)
                     /gene="ansB"
                     /locus_tag="PXB27_002298"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HDL5347040.1"
                     /translation="MKIKKLLLPTLLSIATMPIQAADLPNITILATGGTIAGSQKNTA
                     TSAYQAGQLNIETLIEAVPDIKKLANIKGEQIVKIGSQDMSDEVWLKLAKAINQQCPN
                     TDGFVITHGTDTMEETVYFLDMTVKCEKPVVLVGAMRPATEKSADGVLNLYNSVVVAK
                     DSKSSGRGVLVAMNDIVLGARDVTKTSTTSVQTFNSPNFGALGYIHNSKVDYERSPES
                     KHTVNTPFKVDELTELPKVGIIYAYSNMPTEPLQALLDASYQGIVVAGVGNGNMNAAN
                     LALLEEAAKKGITVVRSSRVPTGYTTRDAEIDDSKYGFIASGTLNPQKARVLLQLALT
                     QTNDIKTIQQYFEDF"
BASE COUNT         1389 a          791 c          924 g         1391 t
ORIGIN      
        1 taaactatca gaaaaaataa tagagttgct tgtttagtaa tctattagat tgaaaaatta
       61 tgaacaaaaa acttccacca ctcaatgcat tgaaagcatt tgaatctgct gcacgccatt
      121 taaattttac taaagcggca gaagatctct tcgtcacaca agctgcagtg agccaccaaa
      181 ttaaactatt agaagatttt ttaggtgctt ccctgttcca tcgccgtaac cgtttgctgg
      241 aattaactga gttaggcgca caatatttct atgaagttca gccattatta gaacaaattg
      301 ctctcgcaac agataaaatt aggcagcaaa gtagtcgtca aattctgact attagcgtac
      361 cacaaacatt tggtatgcat tggttagtcc cacgtttggc ggatttccac gaaaaatatc
      421 cagaagtgga agtacgtatt aagggtgttg atcaagatga gggattatta ggtaaagaaa
      481 cagatgttgc tatttattat ggtaaggggg attggcaagg tctggaatct atccgtttga
      541 cggaatcccg tattgttatt ttagcttcgc ctgagtattt atcgcaaaat accattcatt
      601 taccggcgga tttagcaggt aagcatttat tacattcctc cacccataat aagtggaaaa
      661 gaatggttga acatttgcat atagaagata tagtagatgc ggaaactggc tcaattttta
      721 gccatacttt tatggtgctt caagctgcaa tgcaccaaca aggcattgcg attgccaacc
      781 agttgttggc acagcacgaa attgaaaaag gtactttaat agagcctttc ccaaccacat
      841 tgtttgatga aaaatcgttc tatgttgtct atccttcgca atcagcccaa gttcagaaag
      901 tacaattttt tgttgagtgg attcagcaaa aaatgaaaga gtgttgtgct taagcgtggc
      961 tttatcagcg gtatgcgaaa aattagaaaa atttgaccgc ttgttatcta agttaaaaaa
     1021 ggaatattat gaaatatcaa tgggtattat ttgatgctga tgaaaccttg ttttcattta
     1081 attcttatct tggtttaaaa gcgatgctgg ctcgttacca aattgatttt agtgaacaag
     1141 attatcaaga ctttcaagcg gtaaataaac cactttgggt ggcttaccaa aataatgaga
     1201 tcactgctca agatattcaa accagacgtt ttgtaaaatt atctgctcaa acaggcgtag
     1261 atccgctaca acttaatcaa gagttaatgg cggagatggc attcgtcagt cagcctttag
     1321 aaggtgtaat ggatatgtta aatgctttat atggtaaagt aaaaatgggc attatcacca
     1381 atggttttac cgatttgcag caaaaacgtt tagataatac tcaaacttca catttttttg
     1441 atattgtagt gatatcagaa caaattggtg ccgcaaagcc tgatcgccaa gtgtttgatt
     1501 acgcttttgc attaatggac gaatttgatc gtactaaagt gcttatggta ggcgataccc
     1561 tcgcttctga tattttagga ggtaataatg ctggaattga tacttgttgg ttaaatttgc
     1621 ttagtaaaga gaatgatacg gatattaagc caacttacga agtgcatacg atgcaacaga
     1681 ttattgcgat tgtagagggg aaatcattat gaagctacaa cagttacgtt atatcgttga
     1741 aatagtaaac cagaacttga atattaccga ggccgcagag gtgctttaca cttctcaacc
     1801 ggggattagc aaacaagttc gtttgttgga aagtgagttg ggaattaata tttttgagcg
     1861 taacggtaag cacgttaagg ggctaacgcc tgctggtgaa aaaattgtgg cgattgctcg
     1921 tgaattattg gtaaaagccc agagcattaa atcggttgct gaagaacaaa ctcgacccaa
     1981 caaaggcatc ttacgcattg cgaccaccaa tacccaagcc cgttatatgc taccgagtgt
     2041 aattgagaag tttaaggctc gctatcctga agtaagtttg catttgcacc aaggctcccc
     2101 gaatcaaatt tatgatgctt tggtggctgg agaagtggat ttagccatta ccacagaagc
     2161 tcaatattta tttgatgaag cggttttatt gccgtgttat tggtggaatc gttcagttat
     2221 tgtcaaacca gagcatcctc ttgcggcttt tgtgaaccaa agtaaaaatt tgactgtcga
     2281 acaattaggt agatatgatt tgataaccta tacttttggt tttacagggc aatcggattt
     2341 agatcatgcc ttcaataagg aaggtatttt gcctaatatc gtatttacgg cgacagatgc
     2401 tgatgtaatc aaaacttatg tacgcttagg cttaggggtt ggtattatgg catcaatggc
     2461 acatacggaa acggataaag acttagtcgc tatcaatgca agtcacctgt ttcaaccgag
     2521 tatgacgcaa atcgccttta aacgaggaac ttttcttcgt aactatatgt atgattttat
     2581 taattatttt tcgccacatt taacacgggt acaagtggag aaagcggaac aattacgaga
     2641 taacaatgca atttcaaggt tgttcgaaag tatatcttta gaggagaaat agatgagaat
     2701 ttggcagaag ttagcgagcg taatcgtggt cactttgggc ttgatttcaa acgccagtgc
     2761 ggagaatttt aagttgaaat acagctcaaa ttatttaatg ccagcctaca ttaactttaa
     2821 taacggtggt ggccaatata atattcaggc aaaaattaat gtgcctttat ataatattgt
     2881 cttttctgcg aagggaacag aaaaaaacga acaatttcat atgttgagct accgagatac
     2941 ccgtaacggc aaaacttatg cgatggcaga aattgattct aaaacggtaa aatatggcaa
     3001 agtgaaagag ccactaaaag aagaagcgct gactatgcca acttatgatt tgttcaccac
     3061 agcctttcag ttgagctatt acgataaatt accgagcagt tttcaaacta ctaacggtaa
     3121 aaaactttac ccaacaccga atgtgcagct acgcaaagcc caaaaagagg taaaagtagg
     3181 cggcaaaacc taccaagaaa ttacctacaa gtttagaacc agtgataaaa aagacattac
     3241 ggtgaaaaaa tttgaaggtg aaaaattccc tcgctatatc gcctatagtc gtgatggcga
     3301 caattatgag ctaacttttg atagctttgt aaaataataa agattagtaa tgagacaagc
     3361 ggtctatttt gtgatttgtt tgcaaaatag accgcttgtt atttactttt tagaaatctt
     3421 caaaatattg ttgaatagtt tttatatcat ttgtttgtgt taaggcgagc tgcagtaata
     3481 ctcgagcttt ttgaggattt aatgtgccgg aagcaataaa accatacttg ctatcatcaa
     3541 tttctgcatc tcgggtagta tatccggttg gcactcgaga cgagcgaacc acggtaattc
     3601 cttttttagc tgcttcttca agtaaagcca aattagccgc attcatattg ccattgccaa
     3661 ctcctgcaac cacgatgcct tgataactcg catctaataa tgcttgaagc ggttcggttg
     3721 gcatatttga ataagcataa ataatgccga cttttggcaa ttccgttaat tcatctactt
     3781 tgaaaggggt gtttacggtg tgtttacttt caggagagcg ctcataatca actttgctat
     3841 tatggatata gcctagtgcg ccaaagtttg gtgagttaaa tgtttgcact gatgtagtac
     3901 tggtttttgt aacatcgcga gcgcccaata caatatcatt cattgcaacc aacacccctc
     3961 tgccactaga tttagaatct ttggcgacaa caactgagtt atataaattt aatacgccgt
     4021 cggcactttt ttcagttgct ggtcgcatgg cccccactaa tactacgggt ttttcgcatt
     4081 ttacagtcat atcaaggaag tatacggttt cctccatagt gtctgtgccg tgcgtaataa
     4141 cgaagccatc tgtgtttggg cattgttgat taattgcttt tgccagtttt agccatactt
     4201 catcagacat atcctgagat ccgattttaa cgatttgttc tcctttgata tttgctaatt
     4261 ttttaatatc cgggacggct tctatgagtg tttcaatatt tagctgccca gcttgataag
     4321 ctgaggttgc cgtatttttt tggctaccgg caattgtccc tccagttgca agaattgtaa
     4381 tattaggtag gtcggctgct tgaattggca tagtagcaat gcttaacaga gttggcagta
     4441 gtaatttttt gattttcatt gtatcgtctc cagtattaga aaaacaggta ttatg
//
LOCUS       DANLUQ010000170         4414 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.171, whole genome shotgun sequence.
ACCESSION   DANLUQ010000170 DANLUQ010000000
VERSION     DANLUQ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4414
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.171"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            1..1158
                     /locus_tag="PXB27_002299"
     CDS             1..1158
                     /locus_tag="PXB27_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HDL5347041.1"
                     /translation="MEQSYKIALLFNANKIYDRGVMQGIGQFIQASQCTWDIFVEDDF
                     VYHKNSIESLSIDGIIADFDDLETVELLKDIDVPIVAVGGSYQNPDYYPDLPYVATDN
                     YALVEAAFLHLKQKGINQFAFYGYPAETEKHWAIERKNAFIQLMEKYEHHPNCYIGDQ
                     FHSDNWVELQAKLGEWLQTLPAHTGIIAVTDARARHLLQTCEHLKIAVPEQLCVIGID
                     NEELIQYLSRVSLSSVTQGTSQIGYHAAKLLYRKLNNLPVSTKPILIPPVKVEERRST
                     DYRSLQDPMVIQAMHFIRHRACQGIKVEQVLDHLKISRSNLEQRFKQEMNKTIHQVIH
                     EEKLARATYMLKFTEISTQEIANVCGYPSLQYFYAVFKKEFGKTPKEFRQE"
     gene            1251..2249
                     /gene="gntR"
                     /locus_tag="PXB27_002300"
     CDS             1251..2249
                     /gene="gntR"
                     /locus_tag="PXB27_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HDL5347042.1"
                     /translation="MRKPKRPTLQDIATHLGITKMTVSRFLRDPNTVAKETGERIAQA
                     IELFGYIPNRAPDILSNAKSRAIGVLVPSLTNQVFADVIKGIEQITDQASYQTMLAHY
                     GYSEQKEEQRIESLLSYNIDGIILSENHHSPRTLKMLEVANIPVIEIMESGECGIQQA
                     VGFDNISAAQAMVETMIHRGCRQVVYFGARMDKRSQLKMQGYEQAMLKHGLSPHSITT
                     AEHSSFTLGASQLKQALQHIPQLDGIFCTNDDLAIGALFECQRLGIHVPQQIKIAGFH
                     GHDVGQSLTPQLASVITPRLEIGRVAAQELLNRIHGVPQQSSIINLGYQIHLGESV"
     gene            complement(2303..2818)
                     /locus_tag="PXB27_002301"
     CDS             complement(2303..2818)
                     /locus_tag="PXB27_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDL5347043.1"
                     /translation="MANGKAFILMGVSSTGKTSVGTAVAQRLGIKLIDGDDLHPRANI
                     LKMASGTPLNDADRTPWLERINDAVFSFEQKSEKGIIVCSALKKKYRDQIRQGNADVT
                     FLFLHGSFELVLARMQQRKGHFMKPEMLQSQFNTLEIPQADEPDVIFIDIDGSFDEVV
                     ERCVKAVEPLL"
     gene            3005..4354
                     /locus_tag="PXB27_002302"
     CDS             3005..4354
                     /locus_tag="PXB27_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HDL5347044.1"
                     /translation="MLLFIMFAAIILLLVLIMKFKVHAFVALIIVSLLTALAAGIPVD
                     KILPTLLNGFGSTLASVALLVGLGAMIGRLLEITGGAKVLADTLINKFGKERAPFALG
                     VASLLFGFPIFFDAGLVVMLPIIFSVAKQFGGSVLRYAFPSAGAFAVMHAFLPPHPGP
                     VASGDLLGVNMGLLVLVGLVCAIPTWYIGTYLFSQFISKRIHVDLPKAFLNGLSANEM
                     AVQNPPSFGKVLTVLVLPIILILFDTGLNTLSVAKVIDGSEMWVQSLRLIGKTPVALL
                     ITLIVAIMLLRDNRSFDQIEKICNNALGPICSIVLVTGAGGMFGGVLRASGIGDVLSE
                     MMKDTGMPIVVAAFIIATVFRVAQGSATVALTTTAALIAPTVAAATDLSQFDLCFIVI
                     AIASGATVLSHVNDSGFWLMSRFLEMDEKTTLKTWTMLETAIGLTGFACALIGSWLL"
BASE COUNT         1161 a         1019 c          989 g         1245 t
ORIGIN      
        1 atagaacaat cttataaaat tgccttactt tttaatgcta acaaaattta tgacagagga
       61 gtaatgcagg gcattgggca gtttattcaa gcatcacaat gtacttggga tatttttgtg
      121 gaagatgatt ttgtctatca taaaaatagt atcgaaagcc tgtcgattga cggcattatt
      181 gccgattttg acgatttgga aacagttgag ctacttaaag atattgacgt ccccattgta
      241 gcagtaggcg gttcttacca aaaccctgac tattaccctg accttcctta tgttgctacc
      301 gacaattacg ccttagttga ggcagcattt ttacacctaa aacaaaaagg aatcaaccaa
      361 tttgcgtttt acggctatcc tgcagaaacg gaaaaacatt gggcgataga gcgaaaaaat
      421 gcctttattc agttgatgga aaaatatgaa catcacccaa attgttatat tggtgatcaa
      481 tttcattcag ataattgggt tgaattacaa gccaaattag gcgagtggtt acaaacctta
      541 ccagcccaca cgggcattat tgccgttact gacgcaaggg caagacacct gcttcaaacc
      601 tgtgagcatt taaaaatcgc tgtacctgaa caactttgcg tgatcggcat tgataacgaa
      661 gagttgattc aatacctctc tcgggtttct ctttcttcag tcacacaagg aacatcacaa
      721 atcggctatc acgctgcgaa gttattatat cgaaaattaa ataatctacc ggtttcaacc
      781 aagccgattt taatcccgcc ggtaaaagtg gaagaacgcc gttcgaccga ttatcgttct
      841 ttgcaagacc caatggtaat ccaagctatg cactttattc gtcaccgagc ttgccaaggc
      901 attaaagtag agcaagtact ggatcatctt aaaatttccc gctctaacct tgaacagcgt
      961 tttaagcagg aaatgaataa gacaattcac caggtgattc acgaggaaaa attagcaaga
     1021 gcaacatata tgctcaaatt taccgagatt tcgacccaag aaattgccaa tgtgtgcggt
     1081 tatccctctt tacaatattt ctatgccgtg tttaaaaaag agtttggtaa aaccccgaaa
     1141 gagttcaggc aagagtagta acaagcggtc aatttcaccg aatattttgc aattattgcc
     1201 ttatcttcag ttataatcag cgaaattaaa ttttttcgtt atctattcca atgagaaaac
     1261 cgaaacgccc aactttgcaa gacatcgcta cccatctcgg cattaccaaa atgacggtca
     1321 gtcgcttttt gcgggatcca aacaccgttg ccaaggagac cggcgagcgg attgcacaag
     1381 cgattgagct gtttggctat atcccaaacc gtgcaccgga tattctctcg aatgccaaaa
     1441 gccgtgcaat tggcgtgctg gttccttcgc ttaccaatca agtgtttgcc gatgtgatta
     1501 aaggcattga gcagattacc gaccaagcca gctatcaaac catgctcgcc cactatggtt
     1561 acagcgaaca aaaggaagag cagcggattg agtcgctgct ttcgtacaat attgacggca
     1621 ttatcttatc ggaaaatcac cactcgcccc gcaccttgaa aatgctggaa gtggcgaata
     1681 ttccggtgat tgagataatg gaaagcggcg aatgtggcat tcaacaagcg gtcggatttg
     1741 acaatatttc tgcggctcaa gcaatggtcg agacaatgat tcatcgaggc tgccgacagg
     1801 tggtgtattt cggggcgaga atggataaac gctctcagct gaaaatgcag ggctacgaac
     1861 aggcaatgct caaacacggc ttaagcccac acagcattac tactgcagag cattcttctt
     1921 tcacgcttgg cgcaagccag ctaaaacaag ccctgcaaca cattccgcag ctagacggca
     1981 ttttttgcac caatgacgat ttggcaatcg gggcattatt tgagtgccaa cggttaggca
     2041 ttcatgtgcc gcagcagatt aaaatcgccg gctttcacgg acacgatgtc gggcaatccc
     2101 tcacgccaca gcttgccagc gtcatcacac ctcgccttga aataggcaga gttgccgccc
     2161 aagagctgct caaccgtatt cacggcgtac cacaacaaag ctccataatt aacttgggct
     2221 atcagatcca tttgggggaa agtgtgtagc ccggacaagc ggttaaattt gcaaaaaaat
     2281 ccgcaaaatc caccacttgt gattaaagca acggctcaac tgctttcacg caacgctcca
     2341 ccacctcgtc aaaactgccg tcaatatcaa taaatatcac atctggttcg tctgcctgcg
     2401 gaatttccag cgtattaaat tggctttgaa gcatttccgg tttcataaaa tgccctttgc
     2461 gttgttgcat tcgagccagc accagttcaa acgagccgtg caagaataga aaagtcacat
     2521 cggcattgcc ttgtcggatc tggtctcggt actttttctt caatgctgag cagacgataa
     2581 tccctttttc gcttttttgt tcaaaactga acactgcatc gttaatccgc tccaaccacg
     2641 gtgtgcgatc tgcatcattg agtggcgtgc cacttgccat tttcagtata ttggcacgag
     2701 ggtgtaagtc gtccccatca atcaatttta ttcccaaccg ctgtgcgact gccgttccca
     2761 cgctggtttt gccggtgctg gacacgccca tcaaaataaa tgctttgccg tttgccattg
     2821 aatttccttg tgttactggt aacgttaact tagccgttaa tgtaacaggt tatgctgaaa
     2881 aatcatctgc tatttccaga aaacttgaag tagatcacat ttttaaaata aaagccctgc
     2941 aaccgtttac tctaaactgc tttttggtta tgttaccgat aacaattcaa ccttaggaga
     3001 tcctatgtta ctgttcatta tgtttgctgc catcatttta ttgctggtgc tgattatgaa
     3061 atttaaagtc cacgcttttg tggcattgat tattgtgagc ctgctcactg cattagctgc
     3121 cggtattccg gtagataaaa tcctgccaac attgttgaat ggttttggta gtacgctggc
     3181 atcagtggcg ttactggtgg ggcttggggc gatgattggt cgcttgcttg aaatcaccgg
     3241 cggggcgaaa gtgcttgcgg atacgcttat caataaattc ggcaaagaac gtgcgccttt
     3301 tgcgttgggc gttgcctcgc tgttattcgg cttcccgatt ttctttgatg ccggcttggt
     3361 ggtgatgtta ccgattattt ttagcgtggc aaaacagttc ggcggttcgg tgttgcgcta
     3421 tgcgtttccg tctgccggtg cgtttgcggt aatgcacgcc tttctacctc cgcaccccgg
     3481 cccggttgcc tccggcgatc tgcttggtgt gaatatgggg ctgttagtct tggtgggctt
     3541 ggtttgtgca atcccaacgt ggtatatcgg cacttacctg ttcagccaat ttatcagcaa
     3601 acgcattcac gttgatttgc cgaaagcgtt tttaaacggc ttatcggcaa acgaaatggc
     3661 agtacaaaac ccgccaagtt ttggcaaggt actcacggtg ttggtgctgc cgattatcct
     3721 gattttgttt gataccggct tgaataccct ctccgttgct aaagtgattg atggcagtga
     3781 aatgtgggtg cagagccttc gcttaatcgg caaaacccct gttgctttac tgattacctt
     3841 aattgtggcg attatgctgt tgcgggataa tcgcagtttc gaccaaatcg aaaaaatctg
     3901 taacaatgcc cttggcccaa tctgctcgat tgtcttagta accggtgcag gcggaatgtt
     3961 tggtggcgtg ttgcgtgcca gcgggattgg cgatgtatta tcggaaatga tgaaagacac
     4021 cggaatgccg attgtggttg ccgcctttat tattgctacg gtattccgtg tcgcacaagg
     4081 ctcggcaacc gttgctttaa ccaccacagc agcgttgatt gcgccaactg tggcggcagc
     4141 gactgattta agccagttcg acctctgttt tatcgtgatt gctatcgcct ccggtgcgac
     4201 ggtgctttcc cacgtcaatg actccggttt ctggctgatg agccgtttcc ttgaaatgga
     4261 cgaaaaaact accctcaaaa cgtggacaat gcttgaaact gccatcggtt taaccggttt
     4321 tgcctgtgca ttgattggca gctggttact ctaattccaa ccctgacaag cggtctgttt
     4381 tcttctaaaa tttgcaaaat ttggcagaga atcg
//
LOCUS       DANLUQ010000171         4341 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.172, whole genome shotgun sequence.
ACCESSION   DANLUQ010000171 DANLUQ010000000
VERSION     DANLUQ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.172"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            202..2136
                     /gene="iolD"
                     /locus_tag="PXB27_002303"
     CDS             202..2136
                     /gene="iolD"
                     /locus_tag="PXB27_002303"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015433647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HDL5347045.1"
                     /translation="MQTTRLTVAQALVQFLDNQYLDVDGKEIKFVHGVAAIFGHGNVL
                     GIGQALAQNAGGLTLYQGRNEQGMAHLAMGFAKQNLRQRIIACTSSVGPGAANMVTAA
                     ATATANRIPLLLLPGDVFATRQPDPVLQQIEQPYDLSISTNDAFRAVSKYWDRVQRPE
                     QLMSACINAFRVLTDPAETGAVTIALPQDVQAESFDFPNSFLQKRVHRLERTPPTTAM
                     IKQAVTLIQTKKKPLIVCGGGVRYSQAGQILQKFAKQFGIPFAETQAGKSAVVSDHAL
                     NVGGVGETGCLAANRLAKEADLVIGIGTRYTDFTTSSKWIFQNPNVQFLNINVARFDA
                     YKLDGVQLVADAKTALEALSQAIPTDYQAQWENEISQAKADFADEIQRLRAVQYHPDF
                     TPEINDNLDHKAVHNEFIQLTGSQLTQTQVLGIVNETIDNNAIIVAAAGSLPGDLQRT
                     WLSKGEETYHLEYGYSCMGYEVSAAVGVKIARPEQEVYALLGDGSYLMAHTELVTSIQ
                     EGKKINVVLFDNMANGCINNLQIGNGMDSFGTEFRFRNGETNQLNGGLVPIDFAKNAE
                     AYGCKTYRVTNEAELRAALDDAKKQTVSTLIDIKVLPKTMAHGYGCWWHVGVAEVSES
                     ERIQQARQRSEEQLAKARKY"
     gene            2263..3159
                     /gene="iolE"
                     /locus_tag="PXB27_002304"
     CDS             2263..3159
                     /gene="iolE"
                     /locus_tag="PXB27_002304"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011817366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HDL5347046.1"
                     /translation="MKAENIQLGIAPIGWTNDDLPELGAENTFEQCVSEMALAGYTGC
                     EVGNKYPRDVAVLKHKLDVRGIQICNAWFSTFFVDGKREETIAEFIKHRDFLHAMGAK
                     VIGCSEQSRSIQGTTKSVFKEKPIFNDEDWKRMAEGYNELAKLAAEKGMKVCLHHHMG
                     TGIQTPEEVDRYMAEVNDDVYLLFDSGHLYYSEGCQKAMLGVLEKYIDRIIHVHLKDV
                     RDEVMAEVKAKDLSFLEGVKKGTFTVPGDGVIDFRPIFDILEKHDYKGWMVVEAEQDP
                     ALANPFEYALKGRKYIRETAGV"
     gene            3161..4171
                     /gene="iolG"
                     /locus_tag="PXB27_002305"
     CDS             3161..4171
                     /gene="iolG"
                     /locus_tag="PXB27_002305"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005537901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HDL5347047.1"
                     /translation="MLKVGIIGAGRIGRVHSESISKYVKGAEIKAISDVKITEELTAW
                     AKSMGIPEVYDDYKKILQDPEIDAVLVCSSTNTHAPISIEAAQAGKHVFCEKPVDADP
                     AKIREVLSAVEKAGVKFQVGFNRRFDHNFKAIKDRVAAGDIGEPHVIRVTSRDPEAPP
                     IEYVKVSGGMFFDMTIHDFDMIRYLSGSEVEEVFAVGTVLVNPEIGKAGDIDTAVITL
                     KLKNGAIGVIDNSRKAAYGYDQRAEVFGSKGAIHITNDTGSTAVFSGENGVVAEKPKY
                     FFLERYMQSFADEISCFVDSVVNDKPTLVNGNDGLQPVLIALAAKKSLEENRPVKLSE
                     VA"
BASE COUNT         1274 a          934 c         1044 g         1089 t
ORIGIN      
        1 tcaaatttat tctatttaaa ttatcatttc tgtgatctag ctctcacttt gaaaaataag
       61 gcaataactt cgcagaaatg agaactggat cacatctttt tttcgatttc cttgctatta
      121 taaatgaaat aaatttttca tttgtaaaaa tcagtagaaa accgaccgct tgcaaaagcg
      181 atgtaagcca gaggaggctt tatgcagact actcgtttaa cggttgcaca ggcgttagtg
      241 caatttttgg ataatcaata tttagacgtg gacggcaaag agattaaatt cgttcacggt
      301 gttgccgcta ttttcggcca cggcaatgtg ctgggaatag gtcaggcgct tgctcaaaat
      361 gccggtggtt taacgctcta ccaaggcaga aatgagcaag gtatggcaca tttggcgatg
      421 ggctttgcta agcaaaatct gcgtcaaaga attattgcct gtacttcctc ggtcgggccg
      481 ggggcggcaa atatggttac ggctgcggca acggcaacag cgaaccgtat tcctcttctt
      541 ttattaccgg gcgatgtgtt tgcgacccgc cagccggatc cggtactgca acaaatcgaa
      601 caaccttacg atttaagcat tagcactaac gatgcgttcc gtgccgtcag taaatattgg
      661 gatagagtgc aacgccctga gcagttaatg agtgcttgta tcaatgcgtt tcgtgtgctg
      721 accgaccctg ctgaaaccgg tgctgtcacc attgctctgc cgcaagatgt acaagcggag
      781 agttttgatt tcccgaacag cttcctgcaa aaacgagtgc atcgcttaga gcgtacaccg
      841 ccgacaacag cgatgattaa gcaagcggta acattaattc aaaccaagaa aaaaccgctg
      901 attgtctgcg gtggcggtgt tcgctactcg caagcgggtc aaattctgca aaaatttgct
      961 aaacagttcg gcattccatt tgctgaaacc caagcgggta aaagtgcggt ggtgtccgat
     1021 cacgcattga atgtaggcgg agtgggagaa accggttgtt tagcggcaaa ccgtctggca
     1081 aaagaagcgg atttagtgat tggcattggt acacgctaca ccgacttcac gacttcttcc
     1141 aaatggattt tccaaaaccc aaacgtgcag tttttgaaca ttaatgtggc acgttttgat
     1201 gcgtacaaat tagacggcgt gcaactggtt gccgatgcaa aaactgcatt agaggcgtta
     1261 agccaagcca ttcctactga ttatcaagct caatgggaga atgaaatttc acaggcgaaa
     1321 gcggattttg ctgatgaaat tcaacgcctg cgcgctgtgc aatatcaccc tgatttcacc
     1381 cctgaaatta acgataatct tgatcataaa gcggttcaca atgaatttat tcagctcacc
     1441 ggttcccagc tcacccaaac ccaagtgctg ggtattgtga atgaaaccat tgataacaat
     1501 gcgattattg ttgccgccgc cggaagtttg ccgggtgatt tacaacgcac ttggttaagt
     1561 aaaggtgaag agacttacca ccttgaatat ggctactcct gtatgggcta tgaagtgagt
     1621 gcggcagtcg gcgtgaaaat tgcccgcccc gaacaagagg tctatgcctt gctgggggac
     1681 ggctcttact taatggcaca caccgaatta gtgacctcaa ttcaagaggg caaaaaaatc
     1741 aacgtggtgc tgttcgacaa tatggcaaac ggctgtatca acaacctgca aatcggcaac
     1801 ggtatggaca gtttcggtac ggaattccgc ttccgcaatg gcgaaactaa ccaactcaac
     1861 ggcggtttag tgccgattga ttttgccaaa aacgccgaag catacggctg caaaacctat
     1921 cgggtaacta acgaagccga attgcgtgcc gcccttgatg atgcgaaaaa acagaccgtt
     1981 tctaccttaa tcgatattaa agtgttgccg aaaacaatgg ctcacggcta tggctgctgg
     2041 tggcacgttg gtgtcgcgga agtgtcagag agcgagcgta ttcaacaagc ccgccaacgc
     2101 tccgaagagc agttagctaa ggctcggaag tattagttaa aaaaacaact ccccactttg
     2161 agctacgagg ggagaatatc aagcggtcaa atttgcaaaa tttttgataa aaataaccgc
     2221 ttgcatccaa atttatctct ataaatcaaa cgaggtttct ctatgaaagc tgaaaacatt
     2281 caattaggca ttgccccaat cggttggacg aacgatgacc tgccggaact gggagcggaa
     2341 aacactttcg agcagtgtgt gagtgagatg gcacttgccg gctacacggg ctgtgaagtg
     2401 ggtaataaat atccgcgtga tgttgccgtg ttaaaacaca aactggacgt gcgtggcatt
     2461 caaatctgta atgcgtggtt cagcaccttc tttgtggacg gtaaacggga agagaccatt
     2521 gccgagttca tcaaacatcg tgatttcttg catgcaatgg gggcaaaagt gatcggctgt
     2581 tccgaacaga gccgtagtat tcaaggcaca accaaatctg tgttcaaaga aaaaccgatt
     2641 ttcaacgatg aagattggaa acgtatggca gaaggctata acgaattggc gaaacttgcc
     2701 gccgaaaaag gaatgaaagt ctgcctgcat caccatatgg gaaccggtat tcaaacaccc
     2761 gaagaggtcg atcgctatat ggcagaagtg aatgacgatg tctatctgtt gttcgattca
     2821 gggcatttgt actattccga aggctgccaa aaagcgatgc tcggcgtact ggaaaaatat
     2881 atcgaccgta ttatccacgt tcacttaaaa gatgtgcgtg atgaagtcat ggcggaagta
     2941 aaagcaaaag atttaagctt tttagaaggc gtgaaaaaag gcacctttac tgtaccgggc
     3001 gatggtgtca ttgatttccg tccgattttc gatattttag aaaaacacga ctacaaaggc
     3061 tggatggttg tagaagccga acaagatccg gcacttgcca acccgtttga atacgccctc
     3121 aaaggcagaa aatatatccg tgaaaccgca ggagtttaat atgttaaaag taggtattat
     3181 tggtgcaggc cgtattggac gtgtgcattc tgaaagtatt agcaaatatg tcaaaggtgc
     3241 tgaaatcaaa gcaatctctg atgtaaaaat taccgaagaa ttgaccgctt gggcaaaatc
     3301 gatgggtatt ccggaagttt atgatgacta taaaaaaatc ctgcaagatc cggaaattga
     3361 tgctgtattg gtctgctctt ccaccaacac tcacgctccg atttcgattg aggcagcaca
     3421 ggctggcaaa cacgttttct gtgaaaaacc ggtagatgca gacccagcta aaatccgtga
     3481 agtattgagt gcagtcgaaa aagccggtgt aaaattccaa gtgggcttta accgccgctt
     3541 cgaccacaac ttcaaggcga ttaaagaccg tgtggcggca ggcgacatcg gcgaaccgca
     3601 cgttatccga gtaacctctc gtgatccgga agccccgcca atcgaatatg tgaaagtctc
     3661 cggcggaatg ttctttgata tgaccattca cgacttcgat atgattcgtt atttatccgg
     3721 cagcgaagtg gaagaagtgt ttgcagtcgg caccgtatta gtaaaccctg aaatcggcaa
     3781 agcgggcgat attgatactg ccgttatcac gttgaaactg aaaaacggag caatcggcgt
     3841 aatcgacaac agtcgcaaag ctgcctacgg ctacgaccaa cgtgcggaag tgttcggctc
     3901 gaaaggggca atccatatca ctaatgatac cggctcaact gccgtattct caggtgaaaa
     3961 cggcgtggtg gcggaaaaac caaaatactt cttcttagaa cgttatatgc aatctttcgc
     4021 cgatgaaatc agctgtttcg tagattctgt ggtaaacgac aaaccaacgt tagtaaacgg
     4081 caatgacggc ttacaaccgg tacttattgc cttagctgcg aaaaaatcac tggaagaaaa
     4141 ccgaccagtg aaattgagtg aagtggcgta attgatcata aatacaagcg gtcggtttta
     4201 ctcgctacac tcgcacttcg tgccatcggc atagccgatg ttcaaacgca agcattttgc
     4261 agaaaaattt gtaaaactga ccgtttgagt ttttatttat tatcttttgg gaaataaata
     4321 taatcgtgct caatttttat t
//
LOCUS       DANLUQ010000172         4247 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.173, whole genome shotgun sequence.
ACCESSION   DANLUQ010000172 DANLUQ010000000
VERSION     DANLUQ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.173"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            67..1275
                     /locus_tag="PXB27_002306"
     CDS             67..1275
                     /locus_tag="PXB27_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDL5347048.1"
                     /translation="MSKNLILILNCGSSSLKFAILDPKTGDEKLSGLAEAFHLEDARI
                     KWKLNGEKGNADLGAGAAHSEALTFIAEKLLSDELKAGIGAIGHRIVHGGEKFTSSVV
                     ITDEVIKGIEDATQFAPLHNPAHLIGIKEAFRIFPELKDKNVAVFDTAFHQTMPEHAF
                     LYALPYTFYKENGIRRYGFHGTSHFYITSQVAELAGKSVEQTNAIICHLGNGGSVSVV
                     RNGKCIDTSMGLTPLEGLVMGTRSGDLDPAIISFLYNNLGMSMDEIDTTLVKKSGLLG
                     LTGVTSDCRYAEDNYNDVSKPEAKRALDVYSYRLAKYIGSYMAILGDDHLDAIAFTGG
                     IGENSGLVRELTLNHLKLFGIKLDQERNLAARFGKDGVITADESTFKAVVIPTNEELV
                     IAQDTAKLAL"
     gene            1328..3463
                     /gene="pta"
                     /locus_tag="PXB27_002307"
     CDS             1328..3463
                     /gene="pta"
                     /locus_tag="PXB27_002307"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005542370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDL5347049.1"
                     /translation="MSRTIILIPISTGVGLTSVSLGLVHALEQKGSKVGFLKPISQPI
                     SGEDTLDRSTTIIRAEQTTEVGEPFMLSEAETLIGQNQTDVLLEKVVERHQKLSKNNE
                     IVIVEGLIPTRKNSYANSINYEMAQALDAEIVLVAAPASDTPAQLKERVEAAASQFGG
                     RNNPNLLGVIINKFNAPVDDSGRTRPDLTEIFDSFQHSNNNVSEVEHLFAKSPIKLLG
                     CISWKAELIATRAIDVAKHLGASIINEGEIKARRIRGVTFCARNLPNMVDHFKAGSLL
                     VTSADRPEVLVAASLAVMNGIEIGAILLTGGYKIDSSIAKLCQQAFETGVPVFRIEGN
                     TWQTALSLQSFSLEVPVDDKERIAAIKEYMASKFNVGFIDEVSKAATRARRLSPAAFR
                     YQLTEYARQAKKRIVLPEGDEPRTVKAAALCAERGIAECVLLANPTDVQRVAEAQGVV
                     LGKGVTIIDPASIRENYVDRLVELRKAKGMTEVVAREQLLDTVVLGTMMLEANEVDGL
                     VSGAVHTTANTIRPPMQIIKTAPGSSIVSSIFFMLLPDQVLVYGDCAVNPDPTAEQLA
                     EIAIQSAESAKAFGIDPRVAMISYSTGTSGSGADVEKVKEATRIAQEKRPDLIIDGPL
                     QYDAAIMEDVARSKAPNSPVAGKATVFVFPDLNTGNTTYKAVQRSADLVSIGPMLQGM
                     RKPVNDLSRGALVDDIVYTIALTAIQATQ"
     gene            3577..>4247
                     /gene="can"
                     /locus_tag="PXB27_002308"
     CDS             3577..>4247
                     /gene="can"
                     /locus_tag="PXB27_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HDL5347050.1"
                     /translation="MEKIERLFANNHAWATKMKDEQSDYFKELAEHQKPTYLWIGCSD
                     SRVPAEKLTGLGPGELFVHRNVANMVIHTDLNCLSVVQYAVDVLEIEHIIICGHTNCG
                     GINAAIAQKELGLINNWLLHIRDIWFKHSHLLGKLPYEERSNVLTRLNVAEQVYNLGQ
                     SSIIKTAWEQGKSLSLHGWVYDVTDGFLIDQGVMATSRETLEITYRNAIAKITTEANE
                     KIVLN"
BASE COUNT         1331 a          792 c          864 g         1260 t
ORIGIN      
        1 ccttaaatac accaataaaa atagtatttt cttcaaactc atttcataaa ataaggtaat
       61 ttgaccatgt ctaaaaactt aatcttaatc cttaactgcg gtagttcatc tcttaaattt
      121 gctattttag atcctaaaac aggtgatgaa aaattatctg gacttgctga agcttttcat
      181 ttagaagatg cacgtatcaa gtggaagtta aacggggaga aaggtaatgc tgatttaggt
      241 gcaggtgccg cacatagtga agcacttacc tttattgctg aaaaattgtt atctgacgaa
      301 ttaaaagcag gtatcggcgc gattggtcat cgtattgttc atggcggtga aaaattcacc
      361 tcatcagttg taattaccga tgaagtgatt aaaggtattg aagatgcaac gcaatttgca
      421 ccattacaca accctgcgca tttaattggt attaaagaag cattccgcat tttccctgaa
      481 ttaaaagaca aaaacgtagc tgtatttgac actgcattcc atcagacaat gccggaacac
      541 gcattcttat atgcacttcc atatacattc tataaagaaa atggtattcg tcgctacggt
      601 ttccatggca caagccactt ctatatcacc tcacaagttg cagaattagc aggtaagtct
      661 gttgagcaaa ccaatgccat tatttgccac ttaggtaacg gtggttctgt ttctgttgta
      721 cgcaatggta aatgtattga tacctcaatg ggtttaactc cattagaagg tttagtaatg
      781 ggtactcgct ctggtgactt agaccctgcg attatctctt tcttatacaa taacttaggt
      841 atgtcaatgg acgaaattga tactactcta gtaaaaaaat ctggtctttt aggtttaact
      901 ggcgtaacta gtgactgtcg ttatgcggaa gataattaca atgacgtatc aaaaccggaa
      961 gcaaaacgtg ctttagatgt atatagctat cgtttagcta aatatattgg ttcatatatg
     1021 gcaattttag gcgatgacca cttagatgct atcgcattta caggtggtat cggtgaaaat
     1081 tcaggcttag tacgtgaatt aaccttaaat cacttaaaat tatttggcat taagttagat
     1141 caagagcgta accttgctgc ccgttttggt aaagatggag tcattacagc ggacgagtca
     1201 acatttaaag ctgttgttat cccaactaat gaagaactcg tgattgcaca agataccgct
     1261 aaattagcat tataatcata gggggcagat attctgccct ttcactttct tttaatttgg
     1321 aaatattatg tctagaacga tcattcttat ccctatttca actggtgttg gtttaaccag
     1381 tgttagctta ggtttagtac atgctttgga gcaaaaaggt tctaaagtag gatttttaaa
     1441 accgatttct caacctatca gcggtgaaga tacgttagac cgctcaacaa cgattatcag
     1501 agcagaacaa acaacagagg ttggtgagcc gtttatgtta agcgaagctg aaacattaat
     1561 cggtcaaaac caaacggatg tattgttaga aaaagtggtg gaacgccacc aaaaattatc
     1621 aaaaaataat gaaattgtca ttgttgaagg tttaattcca acgcgtaaaa actcatacgc
     1681 taacagcatt aactatgaaa tggctcaagc ccttgatgct gaaattgtat tagttgcagc
     1741 accggcctca gatacaccag ctcaattaaa agagcgagtt gaagctgccg cttcacaatt
     1801 tggtggacgt aacaacccaa atttactggg cgttattatc aataaattca atgcaccagt
     1861 agatgattca ggtagaaccc gtccagattt gactgaaatt tttgattcat ttcaacactc
     1921 aaataataat gtttcagaag tcgagcatct ttttgctaaa agtccaatta agctgttagg
     1981 ctgtattagc tggaaagcag aacttattgc tacacgagca attgatgttg caaaacattt
     2041 aggcgcttca attattaatg aaggcgaaat aaaggctcgt cggattcgcg gtgtgacatt
     2101 ctgtgcacgt aacttaccaa atatggtcga tcattttaaa gccggtagtt tattagttac
     2161 ctctgcggat cgtcctgaag tgttagttgc tgcatcgttg gcggtaatga atggcatcga
     2221 aattggtgct attttactaa ccggtggtta taaaattgat agctcaattg caaaactttg
     2281 ccaacaagca tttgaaaccg gtgtgcctgt attccgtatt gaaggtaaca cttggcaaac
     2341 cgcattaagc ctacaaagct tcagcttaga agtgcctgta gatgataaag agcgtattgc
     2401 tgcaattaaa gaatatatgg caagcaaatt taatgtaggt ttcattgatg aggtttctaa
     2461 agccgctact cgtgcaagac gtttatcacc tgcagcattc cgttaccaat taaccgaata
     2521 tgcccgtcaa gcgaaaaaac gcattgtttt acctgaagga gatgagcctc gtactgtgaa
     2581 agcggctgca ttatgtgctg agcgtggcat tgcagaatgt gtgttattag ctaacccaac
     2641 agacgtacaa cgtgttgctg aagcacaagg cgtggtatta ggtaaaggcg taacgattat
     2701 tgaccctgcg agcattcgtg aaaattatgt tgatcgctta gttgaattgc gtaaagcaaa
     2761 aggaatgact gaagttgttg ctcgtgagca acttttagat acagttgtat tgggtacaat
     2821 gatgcttgaa gctaatgaag tggacggttt agtctcaggc gcagttcaca caaccgctaa
     2881 cactattcgc ccaccaatgc agatcattaa aactgcaccg ggaagctcta ttgtatcttc
     2941 tatcttcttt atgctactac ctgatcaagt attggtatat ggtgactgtg cggtaaaccc
     3001 tgacccaact gcagaacagc tagcggaaat tgcgattcaa tcagcagaat ctgccaaagc
     3061 ctttggtatt gaccctcgtg ttgcaatgat ctcctactct accggtacat caggttcagg
     3121 tgcggatgta gaaaaagtga aagaagcaac tcgtattgcc caagaaaaac gtccagattt
     3181 aatcattgat ggcccattac aatatgatgc ggcgattatg gaagatgttg cccgctcaaa
     3241 agcaccaaat tcacctgtag caggtaaagc aactgtattt gtcttcccgg atttaaatac
     3301 tggtaatacc acctataaag cggtacaacg ttcggcagat ttagtctcta tcggcccaat
     3361 gttacaaggt atgcgtaaac ctgtaaatga cttgtcccgt ggggctttag ttgacgacat
     3421 cgtttatact attgcgttaa ctgcgattca agcaacgcaa taattccctc ccaaatgaaa
     3481 ccaaatgccc ttaaaactaa gggcattttt attttttacc tacccgactt ttcaatttaa
     3541 tcatataatc aagattagtg ttaaaaagga gaatttatgg aaaaaattga acgcttattt
     3601 gccaataacc acgcctgggc aactaagatg aaagatgaac aatcggatta ttttaaagaa
     3661 ttagcggaac atcaaaagcc aacctattta tggattggct gttccgatag tcgtgtgcct
     3721 gctgaaaaac tcacagggtt aggccccgga gaattatttg ttcatcgaaa tgtagcaaat
     3781 atggtgattc acactgatct taattgtctg tcggttgttc aatatgcggt agatgtatta
     3841 gaaattgagc atattattat ctgtggtcac actaattgtg gtggtatcaa tgcggcaatt
     3901 gcacaaaaag aacttggttt aattaataac tggttgttac atattcgtga tatctggttt
     3961 aaacacagcc atttactagg caaacttccc tatgaagagc gatcaaatgt cttgacccga
     4021 ttaaatgttg cagaacaagt ttataattta ggtcagtcct ctattattaa aaccgcttgg
     4081 gaacagggta aatccctctc gctacacggt tgggtatacg atgttacaga tggtttcttg
     4141 attgaccaag gtgttatggc tactagccga gaaacattag aaattaccta tcgtaatgca
     4201 atagctaaaa taacaaccga agcaaacgaa aaaatagttt taaacga
//
LOCUS       DANLUQ010000173         4235 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.174, whole genome shotgun sequence.
ACCESSION   DANLUQ010000173 DANLUQ010000000
VERSION     DANLUQ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4235
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.174"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(3..1409)
                     /locus_tag="PXB27_002309"
     CDS             complement(3..1409)
                     /locus_tag="PXB27_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HDL5347051.1"
                     /translation="MKLTKIVLSVVAVIGAVAVGGSWYTGKQVEEKYQELVVQANNQL
                     KQLSGEFGANIELKDVQIDRHFFISNAKYRLEVDLGNGKKLDFVGNDKLHHGPLPLNR
                     LAKLNLAPVMMSAESKLQAPEALKKLMGEHIGTGFSNISYSGDVEGHFDISPIKHSDE
                     TGHIETTPIKVQYSYDQKLKGVDSTVELESLKIKAEEFDFQIQGIVYDVQTENNESYP
                     NLTLGKGSGKINAIEFKSKDGKLSQIKDVVVKGDNLLKGDRLISTGDLEAATFNIEGV
                     EMGKLKMDMAYDLDAKLTNDITPLLSNPQTLENEKTGELLLQLLAKSFKFHINNFSLE
                     NSKGKVDLALLLNMAQFDPQNLGNMQAVLQALSTSKFTSNINRQYAEDIIRQVSIVTE
                     KLGEEEAKAFAKQQVDAVFLNAGVEQYGLRKVDDNNVKIELTIDNGKVNLNGRELTED
                     ELQMALFMIVMGAGSLGQ"
     gene            complement(1459..2265)
                     /gene="trpA"
                     /locus_tag="PXB27_002310"
     CDS             complement(1459..2265)
                     /gene="trpA"
                     /locus_tag="PXB27_002310"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HDL5347052.1"
                     /translation="MSRFDTLFTALKAKNEGAFVPFVTLCDPGFERSFEIIDTMIKNG
                     ADALELGFPFSDPLLDGPVIQAANKRALDGGYSTDSCFELIAKIRAKYPDIPIGLLLC
                     ANLIFVPTAEVFFQRCQQSGVDAVLIADLPVFAAQEFVPTAEKYGIQPVFICPPNADQ
                     NTIERIAQLTQGYTYLVSRAGVTSAENQAHASNLDNLVESLKQAGAAPILQGFGIAKP
                     EQVKEALRLGADGAISGSAIVKIIERNLADQANLLKELAEFVQVMKAATK"
     gene            complement(2267..3457)
                     /gene="trpB"
                     /locus_tag="PXB27_002311"
     CDS             complement(2267..3457)
                     /gene="trpB"
                     /locus_tag="PXB27_002311"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HDL5347053.1"
                     /translation="MSDTLLNPYFGEFGGMYVPEILVPVLKELEQAFVDAQQDPTFQA
                     EFADLLKNYAGRPTALTLCRNLTKGSKTKLYLKREDLLHGGAHKTNQVLGQILLAKRM
                     GKTRIIAETGAGQHGVATALACAMLGMPCVVYMGAKDVERQSPNVFRMRLMGAEVIPV
                     QKGSCSLKDACCEAMRDWAANYDTTHYLIGTAAGPHPFPTMVREFQKMIGEEIKRQIL
                     AKEDRLPDAVIAAVGGGSNAIGMFADFIEEQSVKLIGVEPAGKGIETGEHGAPLKHGT
                     TGIYFGMKSPIMQDKDGQIEESYSISAGLDFPSVGPQHAYLNATGRAEYVSITDAEAL
                     EAFQALAKNEGIIPALESSHALAYALKMIKQNPEKEQLLVVNLSGRGDKDIFTVDKIL
                     NGGE"
     gene            complement(3469..4221)
                     /locus_tag="PXB27_002312"
     CDS             complement(3469..4221)
                     /locus_tag="PXB27_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL5347054.1"
                     /translation="MKIALVTGVTAGFGLAICKLLLQSGYKVIGTGRRAERLEELHLQ
                     FGDKFLPLAFDIQQPDEVEKALNSRPKAWQQIDLLVNNAGLALGLEPAQKADLNDWYQ
                     MIDTNIKGLVTMTRLVLPEMVERKSGHIINIGSIAGTYPYPGGNVYGGTKAFVKQFSL
                     NLRADLAGTKVRVSNVEPGLCGGTEFSNVRFKGDDEKAAKVYENVQFVRPEDVANIVL
                     WLNQQPEHVNINRVEVMPVAQTSSALNVARNI"
BASE COUNT         1164 a          990 c          754 g         1327 t
ORIGIN      
        1 gattattgcc cgagtgagcc tgctcccatc acaatcataa acagagccat ttgaagttca
       61 tcttcggtca gttcacgtcc gtttaaattc acttttccgt tatctatagt taattcaatc
      121 ttcacattgt tgtcatctac ttttcttaat ccgtactgtt caactcccgc attgagaaaa
      181 acagcatcaa cttgttgctt ggcaaatgct ttcgcctctt cttcgcctaa tttttctgta
      241 actattgaga cttggcgaat aatatcttcc gcatattgac ggttgatatt tgaagtgaat
      301 ttacttgttg aaagggcttg caataccgct tgcatattac caagattttg tgggtcaaat
      361 tgagccatat tcagtaataa agccaaatca actttgcctt tgctgttttc aagtgagaaa
      421 ttattaatat ggaatttaaa tgattttgct aatagttgta ataataactc gcctgttttt
      481 tcattttcta atgtttgagg attagataat aaaggcgtaa tatcattggt taatttagca
      541 tctaaatcgt atgccatatc catttttaat ttacccattt caacaccctc aatgttgaaa
      601 gtggctgcct ctaaatctcc ggtagaaatt aaacgatcgc cttttaatag attatcgccc
      661 ttcaccacaa catctttaat ttgagataat ttgccatctt tagacttaaa ctcaatagcg
      721 ttaatttttc cactaccttt gcctaaagtt aaattaggat aactttcatt attttctgtt
      781 tgaacatcat aaactatacc ttgaatttga aaatcaaatt cttctgcttt tatcttaaga
      841 ctttctagct caacagtgct atcaacccct tttaattttt ggtcgtagct atattgcact
      901 ttgattggcg tggtttcaat atgacctgtt tcatcactat gtttaatcgg ggaaatatca
      961 aaatgccctt ccacatcgcc cgaatagctg atattagaaa aaccggtgcc aatatgttca
     1021 cccatcaatt ttttaagggc ttcaggagct tgtaatttgc tttccgcact catcattaca
     1081 ggagctaaat taagtttcgc taaacgatta agcggtagcg gaccgtgatg aagtttatca
     1141 ttaccaacaa agtccaattt cttaccatta cccaaatcca cttctaaacg atatttcgca
     1201 ttagagataa agaaatggcg atcgatctgc acatctttaa gctcaatatt cgcaccaaat
     1261 tcgccactca gttgtttaag ttggttatta gcttgaacaa ccaattcttg atatttttct
     1321 tcaacctgct tacctgtgta ccaacttccg ccaactgcaa cagcaccaat taccgctaca
     1381 actgaaagaa caatttttgt taatttcatt ctgtttcctt ttattttcaa aaaaatttaa
     1441 aaaatgaccg cttgtagttt acttcgtagc cgctttcatc acttgtacaa attcggcaag
     1501 ttctttgagc aaattagctt ggtcagctaa attacgttca ataattttca caatcgctga
     1561 gcctgaaatc gccccgtctg ccccaagcct taacgcctct tttacctgtt caggtttggc
     1621 tatgccaaaa ccttgtaaga taggggcagc tccggcttgt tttaaacttt ccactaaatt
     1681 atccaaatta cttgcgtgag cttggttttc agcactagtt acaccggcac gggaaactaa
     1741 ataagtgtag ccttgggtta attgagcaat ccgttcaatg gtgttttgat cagcattcgg
     1801 cgggcaaata aacaccggct gaattccata tttttcagcg gttggcacaa actcttgagc
     1861 agcgaaaaca ggcaaatcag caatcaacac cgcatcaact ccactttgtt ggcaacgttg
     1921 gaaaaatact tctgcggttg gcacaaaaat taaattggca cacagtagta aaccgatagg
     1981 aatgtctgga tatttagctc gaattttggc aatcagctca aaacagctat cagtgctata
     2041 gccaccatct aaagctcgtt tattcgctgc ttgaatcacc ggcccgtcta agagggggtc
     2101 agaaaacgga aagcctagct ctaaagcatc agccccgttt ttaatcatcg tgtcgataat
     2161 ctcaaaactg cgttcaaaac caggatcgca taatgtcaca aacggcacaa aagccccttc
     2221 gttttttgct tttaatgcag taaatagcgt atcaaagcgg ctcattttac tctcctccat
     2281 ttaaaatttt atccacggtg aaaatgtctt tatcgcctcg acccgataaa ttcaccacta
     2341 ataactgctc tttttcaggg ttctgcttaa tcatttttaa cgcataggct aaggcgtggg
     2401 aagattctaa cgccggaata atcccctcat ttttggctaa tgcttggaat gcctctaaag
     2461 cctctgcatc ggtaatcgat acatattcag ctcgccctgt cgcatttaaa taagcgtgtt
     2521 gcggcccgac agaagggaaa tctaagccgg ctgaaatgga gtaagactct tcaatttgcc
     2581 cgtctttatc ctgcataatc ggcgatttca taccgaaata gatccccgtt gtgccgtgtt
     2641 tgagcggtgc accgtgttca ccggtttcaa ttccttttcc tgccggctca acaccgatca
     2701 atttgacgga ttgctcttcg ataaaatctg caaacatacc gatagcattt gagccaccgc
     2761 ctacggcagc aatcaccgca tcaggcaagc ggtcttcttt tgctaaaatt tgacgtttga
     2821 tctcttcgcc aatcattttc tgaaattcac gcaccattgt cgggaacggg tgcgggccgg
     2881 ctgcagtgcc gattaggtaa tgggtagtat cataatttgc cgcccaatcc cgcattgcct
     2941 cgcaacaggc atctttgagc gagcaagagc ctttttgtac cggaatcacc tccgctccca
     3001 ttaaacgcat acggaataca tttggcgatt ggcgttctac atcttttgcg cccatataca
     3061 ccacgcaagg cattcctaac atcgcacacg ctaatgcggt tgccacaccg tgctgtcctg
     3121 ctcctgtttc ggcaataatg cgggttttac ccattcgttt agcgagtaag atttgcccta
     3181 gcacttggtt ggttttatgt gccccaccgt gtaaaagatc ttcacgtttt aaatagagtt
     3241 tggttttact gcctttggtt aaattacgac aaagggttaa agcagtcggg cgaccggcat
     3301 agtttttgag taaatcggca aattcagctt ggaatgttgg gtcttgctga gcatctacaa
     3361 aggcttgttc taattctttt agcaccggca ctaaaatttc cggcacatac ataccaccaa
     3421 attcaccaaa ataaggatta agaagtgtgt ctgacataat atttcctttt aaatatttct
     3481 cgctacattt aaggcagaag aagtttgagc caccggcatc acttcaaccc gattaatatt
     3541 aacgtgttca ggttgctgat tcagccaaag cacaatattt gccacatctt ccgggcgcac
     3601 aaactgcacg ttttcataaa ctttagccgc tttttcatca tcgcctttaa agcgtacatt
     3661 tgaaaattcc gtaccgccac atagccccgg ctcaacattg gaaacacgca ctttagtgcc
     3721 ggctaaatct gcccgcaaat ttaggctaaa ctgtttcacg aaggctttag taccaccata
     3781 tacattaccg cccgggtaag ggtaagtgcc ggcaatcgag ccgatattaa taatatgccc
     3841 acttttccgc tccaccattt caggcaaaac taagcgtgtc atcgttacca atcctttaat
     3901 attggtatcg atcatttgat accaatcgtt caaatctgct ttctgtgccg gttctaagcc
     3961 gagtgcaagg cctgcattgt ttactaataa atcaatttgt tgccacgctt tagggcggct
     4021 attcagtgct ttttccacct catcgggctg ttgaatatca aatgcaagcg gtagaaattt
     4081 atcgccaaat tgtaaatgca gttcttctaa acgctctgcc cgtctgcccg tgccaattac
     4141 tttataaccc gattgaagca ataatttaca aattgccaac ccaaatccag cagttactcc
     4201 tgtgactaat gctattttca tcttttcacc ctcaa
//
LOCUS       DANLUQ010000174         4212 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.175, whole genome shotgun sequence.
ACCESSION   DANLUQ010000174 DANLUQ010000000
VERSION     DANLUQ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.175"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            52..975
                     /gene="oppB"
                     /locus_tag="PXB27_002313"
     CDS             52..975
                     /gene="oppB"
                     /locus_tag="PXB27_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HDL5347055.1"
                     /translation="MLKFIFKRLLEAIPTLLILITFSFFLMRLAPGSPFTSEKAYPPK
                     VMANIEAKYHLNEPLYKQYFLYMENLSKGDFGPSFKYKDQSVNNLIASAFPVSIKLGI
                     TAFIFAVLIGLTAGTIAALKQNSKWDYLVMGFSMTGIIMPSFVFAPLLVLMFAIYLKW
                     LPAGGWNGGQLYYMVLPVLSLTISYVAGIARITRGSMIEVLHSNFIRTAKAKGLPMRK
                     IIFKHALRPALLPVITYLGPAFVGIITGSMVIESVFGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGTLTILFNAIVDILYAVIDPKIRYS"
     gene            990..1889
                     /gene="oppC"
                     /locus_tag="PXB27_002314"
     CDS             990..1889
                     /gene="oppC"
                     /locus_tag="PXB27_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HDL5347056.1"
                     /translation="MQNSSKNQPLVEVTSTIQGRSLWQDARRRFFRNKAAVASLIILC
                     CIILFITFAPMLMPFSYEDTDWNMMGIAPDFESQHYFGTDSSGRDLLVRVAIGGRISL
                     MVGIAGALIAVLIGTIYGAISGYFGGKVDMVMMRFLEILSSFPFMFFVILLVTFFGQN
                     IFLIFIAIGMVAWLNLARIVRGQTLSLKNKEFVEAAIVCGVSRRQIIWRHIVPNVLGL
                     VVVYASLEVPSLILFESFLSFLGLGTQEPMSSWGALLSDGAAQMETSPWLLAFPAFFL
                     CLTLFCFNFIGDGLRDALDPKDK"
     gene            1899..3494
                     /locus_tag="PXB27_002315"
     CDS             1899..3494
                     /locus_tag="PXB27_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5347057.1"
                     /translation="MELLAVNNLDVYLKTDEELVHAVRNVSFSIEKGQTLAIVGESGS
                     GKSVTSMAIMQLLPKNIVSFGERSSIIFEEQNLLTLSEKAMQTVRGDRIGMIFQEPMT
                     SLNPYMPIGKQVAETVQTHNPQISNAEAEKMVLETLQKVKIPDAEKKMICYPHEFSGG
                     QLQRIMIAMAIINKPDLLIADEPTTALDVTTQAEILDLMHDLQRDMGMAIILISHDLR
                     LVHKYSDLVCVMQNGEIIERGETETVFTNPQHPYTVELLTPIPTNLKGELPPNAPTLL
                     QGEHIKVDYILKRSLFGKPKKVFNALKDISLHLKTGETLGIVGESGSGKSTLGRAIMQ
                     ILDYRGNITFAGKTLANLNKAERQALKKDMQMVFQDPFNSLSPRLTVGEIIAEGLTVH
                     CPQMSKTERREKVMKMLEEVNLNPAMINRYPHEFSGGQRQRIAIARAIILEPKFVLLD
                     EPTSALDRSTQITVIELLNRLQKKYGLSYIFISHDLAVIKALSDRVIVMSQGEIVESG
                     DVTQIFENPQQEYTKRLILASNL"
     gene            3674..4069
                     /locus_tag="PXB27_002316"
     CDS             3674..4069
                     /locus_tag="PXB27_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL5347058.1"
                     /translation="MKKLMIFATTAMIVSNLAHAAGEQSDAREAHESTVKTSTVKYSC
                     QNGKKLSVKYGFNKQGIPTYAEAKLSGKKRFMPINLYTTDATGTNFGDENNFSLYGDP
                     MEFTNHRKASVNIQSPASEILYKGCTPQK"
BASE COUNT         1219 a          830 c          887 g         1276 t
ORIGIN      
        1 ttaatttacg ctattaattg cctaccaata taactaaaaa gagtggtgct tatgctgaag
       61 tttattttta aacgtcttct tgaggcgatt ccaaccctac taattctgat taccttttcc
      121 tttttcctga tgcggcttgc cccgggtagc ccatttacca gtgagaaagc ctatccgccg
      181 aaagtaatgg caaatatcga ggcaaaatat cacctgaatg aaccgctgta taagcagtat
      241 tttctttata tggaaaatct gtcaaaaggc gatttcggtc catcttttaa atataaagat
      301 caatcggtaa ataatctgat tgcttctgcg tttcctgttt cgataaaact gggcattact
      361 gcctttattt ttgcggtgct tatcggttta accgcaggaa ctatcgctgc acttaagcaa
      421 aatagtaaat gggattattt agtgatgggc ttttcaatga cgggtattat tatgccgagc
      481 tttgtctttg ctccgctgtt ggtactcatg tttgcgattt atctcaaatg gttgcctgcc
      541 ggtggttgga acggtggtca gctctactat atggttttac ctgttctctc tttaaccatt
      601 agctatgttg ccggcattgc ccgtattact cgtggttcaa tgattgaggt gttgcactct
      661 aattttattc gcaccgctaa ggcaaaaggc ttgccaatgc gtaaaattat tttcaaacac
      721 gctttgcgtc cggcattgtt gcctgtgatc acttatttag gcccggcttt tgttggcatt
      781 attaccggct cgatggtaat tgaaagcgtg tttggcttac cgggaatcgg gcagttgttc
      841 gtaaacggag cattaaatcg tgactattca ttagtattaa gcctaaccat tttggtaggt
      901 acgctcacca ttttatttaa tgcgattgtt gatattttat atgcggtcat cgacccgaaa
      961 attcgttatt cataggagta aagcggaaaa tgcaaaattc ttcaaaaaat caaccgcttg
     1021 ttgaggttac ctcaaccatt caaggacgaa gtttatggca agatgcacgc cgccgttttt
     1081 tccgaaataa ggcggctgtg gcaagtttga ttattctttg ttgtattatt ctttttatta
     1141 cctttgcccc aatgctaatg ccgtttagct atgaagatac cgattggaat atgatgggga
     1201 ttgctccgga ttttgaatct caacactatt tcggtactga ttcttcaggg cgtgatttgc
     1261 tggttcgagt tgcaatcggt ggacgcattt cgttaatggt agggattgcc ggtgcattaa
     1321 ttgcggtgct tatcggtaca atctatggag caatttccgg ttatttcggc ggtaaagtcg
     1381 atatggtgat gatgcgtttt ctggaaattt taagctcatt cccatttatg ttcttcgtaa
     1441 ttttgctggt tacgttcttc gggcagaata ttttcctgat tttcattgca atcggtatgg
     1501 tagcgtggct gaaccttgcc cgtattgtac gtggtcaaac tctttcgctt aaaaataaag
     1561 agtttgtgga agcagcgatt gtgtgtggcg tttcccgccg tcaaattatt tggcgacaca
     1621 ttgtgcctaa cgttttaggc ttagtggtgg tttatgcctc ccttgaagta ccatctctga
     1681 ttttgtttga atcgttctta agtttcttag ggcttggtac acaagagccg atgagcagtt
     1741 ggggggcatt attaagcgac ggagcggcac aaatggaaac ctcgccttgg ctactggctt
     1801 tccctgcctt tttcttatgt ttaacactgt tttgttttaa ttttattggt gatggcttgc
     1861 gtgatgccct cgatccaaaa gataaataga ggtttagaat ggaattatta gcagtaaata
     1921 atctcgatgt ttatttaaaa acggacgaag aattagttca cgcagtgcgt aatgtctcgt
     1981 tctcgattga aaaagggcaa acccttgcga ttgtggggga atcgggttcg ggtaaatccg
     2041 ttacttcaat ggcaattatg cagttattgc ctaaaaatat cgtctcattc ggtgaacgct
     2101 caagcatcat ttttgaagaa caaaacttgc ttactctatc tgaaaaagca atgcaaacag
     2161 ttcgaggtga tcgcatcggt atgattttcc aagagccaat gacctcactc aatccttata
     2221 tgccgattgg caagcaggtt gctgagacgg ttcaaaccca taatccgcaa atcagcaatg
     2281 ccgaagcgga aaaaatggtg ttggaaactt tgcaaaaagt aaaaattccc gatgcggaaa
     2341 agaaaatgat ttgctatccg cacgagtttt ccggcggtca gctacaacga attatgattg
     2401 caatggcaat tatcaacaaa cccgatttat tgattgccga tgagccgacc accgccctgg
     2461 atgtcacaac ccaagcggaa attttggatt taatgcacga cttacagcgt gatatgggaa
     2521 tggcgattat cttaatttcc cacgatttac gcttggtgca taaatacagt gacttagtct
     2581 gcgtaatgca aaacggcgaa attattgagc gaggcgaaac ggaaactgtg ttcaccaatc
     2641 cgcaacatcc ttatacggtg gagctactta ccccgattcc aactaattta aaaggggaat
     2701 taccgccaaa tgcaccaacc ttgcttcagg gtgagcatat taaggtagat tatattttaa
     2761 aacgctcgct attcggtaag ccgaaaaagg tgtttaatgc gttaaaagat atttcattgc
     2821 acttgaaaac cggcgaaacg ctcggcattg taggcgaatc cggctccggg aaatctaccc
     2881 ttgggcgtgc cattatgcag attttagatt atcgcggcaa tattacattt gcaggtaaga
     2941 cactggctaa tctgaataaa gccgaacgtc aagccctgaa aaaagatatg caaatggtgt
     3001 tccaagaccc gttcaactca ctttctcctc gcttaacagt tggggaaatt attgctgagg
     3061 gcttaaccgt acattgccct caaatgagca aaaccgaacg gcgggaaaaa gtaatgaaaa
     3121 tgttggaaga ggttaatctc aaccctgcaa tgattaaccg ctatccgcac gaattttcgg
     3181 gcggacaacg ccagcggatt gcgattgcaa gggcaatcat tttagagccg aaatttgtat
     3241 tgcttgatga gccaacctca gcacttgacc gctctactca gatcactgta attgaattgc
     3301 ttaaccgctt acagaaaaaa tacggcttaa gttatatttt catcagccac gatttggcgg
     3361 tcatcaaggc acttagcgac cgagtgattg taatgagcca aggtgagatt gtagaaagcg
     3421 gtgatgtcac tcaaattttt gaaaatccac aacaagaata caccaagcgg ttgattttag
     3481 cctctaattt gtaacccctt ccacaaggtc ttattctacc aaaaaacaag accttgtcat
     3541 tttatgctag caaaacattg taattttgat attttatttc aattttttga aaaaatatgg
     3601 aactttatat aaaactcact ttctaaacaa tacgttatct gaactaagat aacgcttatt
     3661 taaaggaaaa tatatgaaaa aattgatgat attcgcaaca actgccatga ttgtaagcaa
     3721 tttagcccat gcagcaggtg aacaaagtga tgcaagagaa gcccacgaaa gcaccgtaaa
     3781 aacctcaact gttaagtata gctgccaaaa cggcaaaaaa ttaagcgtga aatacggctt
     3841 caataaacaa ggcattccaa cctatgctga ggcaaaatta agcggtaaaa aacgttttat
     3901 gccgatcaac ctatacacga cagatgcaac cggcactaac tttggtgacg aaaacaattt
     3961 cagcctttac ggtgatccaa tggaatttac caaccaccgt aaagcatcag taaatatcca
     4021 aagccccgca agtgaaattt tatataaagg ttgcacacct caaaaataga taaaaagtaa
     4081 aatgagtccg ctctgataag tggcttttta atagctgttt attgcagata ggaaagcatt
     4141 ttgtttttat ctactatcca gaaacaagcg gttaaatttg tttaattttt tacaaaaaat
     4201 gggccaaaat ta
//
LOCUS       DANLUQ010000175         4183 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.176, whole genome shotgun sequence.
ACCESSION   DANLUQ010000175 DANLUQ010000000
VERSION     DANLUQ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.176"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            9..1583
                     /gene="murJ"
                     /locus_tag="PXB27_002317"
     CDS             9..1583
                     /gene="murJ"
                     /locus_tag="PXB27_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDL5347059.1"
                     /translation="MSKKLLRSGMIVSFMTLISRILGLIRDIVVATILGTGVSADIFL
                     FANRIPNFLRRLFAEGAFSKAFVPVLAEYNADNDPNKTREFIAKVSGTLGGLVTVVTL
                     VAMIASPVVAALFGTGWFLDWLYDGPNAEKFTQASFLLKITFPYLWFITFVALSGAVL
                     NTIGKFGVMAFSPVLLNVAIIAVALWGRDYFDSPDIALAWGVFLGGLLQFLFQIPFMK
                     KEGLLVKPKWAWKDEGVTKVRKLMIPALFGVSVTQLNLLLNQVIASFLITGSISWMYY
                     ADRLIEFPLGLFGIAISTVVLPSLSRIAKNKELTEIQRGEHFQNTMDWGVRMVLLLGI
                     PAMIGMAVLAQPIIMTMFMRGKFGFEDVLATSYPLWVMCLGLNSYMLISVLANGFYAN
                     QNTKTPVKVGIIAALSNICFGLAFAPFLGYIGLALASACSALVNVSLLYVNLSKNGYY
                     KVSHKTVLFVLKLFIAACVMGALVAYFTPEIELWALMTMWQKVYWLIWLMILAGGSYF
                     ASILVLGIRKKDFRSA"
     gene            1585..2052
                     /locus_tag="PXB27_002318"
     CDS             1585..2052
                     /locus_tag="PXB27_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HDL5347060.1"
                     /translation="MKSLKLKIPPPAWFLLCAALMWLVAQYVPVSLPNYQHTAIFFSG
                     LAVAIIGGAVAVSALWVIHQAKTTYSPFRPQHTAKLVTWGIYRKSRNPMYLSLLLGLI
                     AWALWLGAMLAWLVLPLFVWLITYFQIKPEEQILAQKFGTHYAKYCNQVRRWL"
     gene            complement(2117..4126)
                     /locus_tag="PXB27_002319"
     CDS             complement(2117..4126)
                     /locus_tag="PXB27_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HDL5347061.1"
                     /translation="MDLIKKLNTGSTFRVPIFLPSLLFVSFVAVFCIIFPQQAQTSLD
                     TIKNSLFQHFSWFYIFAGSIFFLFLIFLSFSRLGDIKLGADTDEPEFGFGSWIAMLFA
                     AGMGIGLMYFGVAEPILHYLKPVQQNLTEPERMKEAMMTTFYHWGIHAWAIYGVIALA
                     LAYFGFRYKLALTIRSGFYPLLKHRISGFWGHLIDIIALCSTIFGLTTTLGFGVMQVS
                     AGFNNLGLIEQSNFTVLAIIVTVAMALAVLSAVSGVGKGVKILSEINLTLAGLLLIFV
                     IITGPTLLLFSSFTENLGYYFSSLLEMSFRTFAYEPEHQGWLSGWTVLYWAWWASWAP
                     FVGLFIAKISKGRTIREFILGVLFVPSLFNILWMTSFGSSAIWFDQQTAGALAEVSGN
                     TEQLLFTFFEQLPFGSIASFVAVIVISIFFITSADSGIFVLNSIASQGEENAPKWQSV
                     LWGALLAILALSLLYSGGLASLQTMTLIIALPFTFIMLILCIGLWKGLMVDNQYFNKK
                     FSQGSQHWAGKDWKQRLEKIINPSNKQDVRHFFIKVARPAFLELIEEFESYGLIAKMN
                     FTNEQNPKLEFEVVKENLRNFIYGIESVPRELSDLVVGDDNLPNIEQNTIYEPITYFL
                     DGRKGYDVQYMTKEELIADVLQQYERFINLAMDNSHDLMTADFNH"
BASE COUNT         1181 a          837 c          934 g         1231 t
ORIGIN      
        1 tctttattat gagcaaaaaa cttttaagat caggaatgat cgtaagcttt atgaccttaa
       61 tttctcgcat actcggtttg attcgagaca ttgtggtggc aaccatttta ggcacaggcg
      121 tgagtgccga tattttcctg tttgccaacc gtattcccaa ttttcttcgc cgtttgtttg
      181 ccgaaggggc attttctaaa gcctttgtgc cggtgttggc ggaatataat gcggataacg
      241 atccaaacaa aacgagagaa tttattgcca aagtatcggg tacattagga ggtttggtaa
      301 ctgttgttac ccttgtggca atgattgcct cgcctgtggt tgccgctctt tttggtacag
      361 gttggttttt agattggctt tacgacgggc caaatgccga aaaattcacc caagcctctt
      421 ttttgctcaa aatcactttc ccttatttgt ggtttattac ctttgtggcg ttatccggtg
      481 cggtgttaaa taccatcggt aagtttgggg tgatggcatt ttccccggtg ttgctgaatg
      541 tggcgattat cgcggtagcg ttatggggca gagattattt cgattccccg gatattgcct
      601 tagcgtgggg ggtattttta ggcggcttat tgcaattttt attccaaatt ccatttatga
      661 aaaaagaggg cttactggtt aagccaaaat gggcgtggaa agacgaaggt gtcactaaag
      721 tccgtaaatt aatgattccg gctttatttg gcgtttccgt tacccagcta aatttactgc
      781 taaaccaagt gattgcctcg tttttgatta ccggctcgat tagttggatg tattatgccg
      841 accgcttaat tgaatttccg ctcggtttat ttggaattgc gatttccacc gtcgtgctgc
      901 caagcctttc ccgtattgcg aaaaacaaag aactcactga aatccaacgt ggagaacact
      961 ttcaaaacac aatggattgg ggggtgagaa tggtgctgtt gctcggcatt ccggcaatga
     1021 tcggtatggc agtgctggct cagccgatta ttatgacaat gtttatgcgg ggtaaatttg
     1081 gttttgaaga tgtgctggca acctcttatc cactttgggt aatgtgcttg gggttaaaca
     1141 gctatatgtt gattagcgtg ctggcaaacg gtttttatgc caaccaaaac accaaaacac
     1201 cggtaaaagt tgggattatt gccgccttaa gtaatatttg ctttggcttg gcgtttgctc
     1261 cgtttttggg atacattggt ttagcgttag cttctgcgtg ttcggcatta gtgaatgtga
     1321 gcttgctgta tgtgaatctc tcaaaaaatg gttactataa agtaagccat aaaaccgtgc
     1381 tttttgtgtt gaaactattc atcgccgctt gtgtaatggg ggcgttggtt gcctatttca
     1441 cgcctgagat tgaactttgg gcattaatga caatgtggca gaaagtgtat tggctgattt
     1501 ggctgatgat cttagccggc ggtagttatt ttgcctctat tttggtatta ggcattcgta
     1561 aaaaagattt ccgctcggca taaaatgaaa tcattaaaac tgaaaatccc gccgccggct
     1621 tggttcttgc tttgtgccgc tttaatgtgg ctggtagctc aatatgtgcc ggtgagcttg
     1681 ccgaattatc agcatacagc catatttttt agcggtttag ctgtggcgat aattggcgga
     1741 gcggttgccg tctcggcact ttgggtcata caccaagcga aaacgacata cagtcctttt
     1801 cgaccacaac ataccgctaa attggtcact tggggcattt accgcaaaag ccgaaaccca
     1861 atgtatcttt cgttactatt aggtttgatt gcgtgggcat tatggttggg ggcaatgttg
     1921 gcgtggctgg ttttaccgct gtttgtatgg ttgataacct atttccaaat caagccggaa
     1981 gagcaaattt tagcccaaaa attcggcaca cattatgcca aatattgcaa ccaagtaagg
     2041 cgttggcttt aaatgcaagc ggtgagattt tgttggaaat ttgcaaattt ttccgaaaat
     2101 ctcaccgctt gtggggttag tgattgaaat cagccgtcat taagtcgtgc gagttgtcca
     2161 tcgctaaatt gataaagcgt tcatactgtt gcagcacgtc ggcaatcaac tcttctttgg
     2221 tcatatattg cacatcataa cctttccgcc cgtctaagaa ataagtaatc ggctcgtaaa
     2281 tggtattttg ctcaatgttc ggtaggttgt cgtcacctac caccaaatcc gataattccc
     2341 gtggcacact ttcaatgccg taaatgaaat tgcgtaaatt ttctttcacc acttcaaact
     2401 ctaatttcgg gttttgttcg ttggtgaaat tcattttagc gattaagcca tagctttcaa
     2461 attcctcgat aagttctaaa aatgctggtc tggcaacttt aataaagaag tgacggacat
     2521 cttgcttatt gcttgggttg atgattttct ccaagcgttg tttccaatct ttacccgccc
     2581 aatgttggct accttgcgag aattttttgt tgaagtattg gttatctacc attaatcctt
     2641 tccataagcc gatacagaga atcagcataa tgaaggtaaa tggtaaggcg ataatcagtg
     2701 tcattgtttg cagagaagcc aagccacccg aatagagtag tgataacgct aagatggcta
     2761 ataatgctcc ccaaagcacg ctttgccatt tcggtgcatt ttcttcgcct tgtgaagcaa
     2821 tgctgttgag aacaaaaatc cccgagtcgg cagaggtgat aaagaaaata ctgataacaa
     2881 tgacggcaac gaaagaggca atagagccaa acggtaattg ctcaaaaaag gtaaataaca
     2941 gttgttcggt attgccgctg acttcagcta aagcaccggc agtttgttga tcgaaccaaa
     3001 tggcagagct gccgaagctg gtcatccata aaatgttaaa cagcgatgga acaaatagca
     3061 cccctaaaat aaattcacga atggttctgc ctttagagat cttggcaata aacaaaccaa
     3121 caaatggcgc ccaagatgcc caccatgccc aataaaggac cgtccagccg cttagccagc
     3181 cttgatgttc cggttcataa gcgaaggtac ggaaactcat ctcaagcagc gagctaaaat
     3241 aatagcctaa attttcggtg aagcttgaga aaagtaatag agttgggccg gtgattatca
     3301 caaaaataag tagcaatccg gctaatgtga gattgatttc acttaagatt ttaacccctt
     3361 tgcctacgcc cgaaacggca gataacacgg caagagccat tgctactgtt acgataatcg
     3421 caagaacagt aaaattgctc tgttcaatta aacctagatt gttaaagcca gcactgacct
     3481 gcatcacccc aaagccaagt gtagtcgtta aaccgaaaat cgtgctacaa agggcaataa
     3541 tatcaattaa atgcccccag aagcctgaaa tacgatgttt tagtaaggga taaaatccgg
     3601 aacgaatagt gagtgctaac ttatatctga agccaaaata agcaagagct aaggcaatca
     3661 caccataaat tgcccaagcg tgaatacccc aatgatagaa cgttgtcatc atcgcttctt
     3721 tcatacgctc cggctcagtt aaattttgtt ggacgggttt aaggtaatgc aaaataggtt
     3781 ctgctacccc aaaatacatt aacccgatcc ccattccggc ggcgaataac atcgcaatcc
     3841 aagagccaaa accaaattca ggctcatcgg tatctgcccc taatttaata tcacccaatc
     3901 ggctgaaaga gagaaaaatt agaaacagga aaaagataga gcctgcaaaa atatagaacc
     3961 agctaaaatg ttggaagaga ctatttttga tggtatcaag tgaggtttgt gcttgctgtg
     4021 gaaagatgat acagaaaacg gcaacaaagc tgacaaagag taaactcggt aggaaaatcg
     4081 gtaccctaaa ggtacttcct gtgtttaatt ttttgattaa atccaatgct atttcctcta
     4141 tttttgaatg gttagaggct tgaatatagc aaaaagatat aaa
//
LOCUS       DANLUQ010000176         4110 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.177, whole genome shotgun sequence.
ACCESSION   DANLUQ010000176 DANLUQ010000000
VERSION     DANLUQ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.177"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(46..1761)
                     /gene="proS"
                     /locus_tag="PXB27_002320"
     CDS             complement(46..1761)
                     /gene="proS"
                     /locus_tag="PXB27_002320"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDL5347062.1"
                     /translation="MRTSQYLFSTLKEMPNDAQVVSHQLMLRAGMIRPMASGLYNWLP
                     TGIKVLKKVENIIREEMNKGGAIEVLMPVVQPAELWVESGRWNDYGPELLRFKDRGER
                     DFVLGPTHEEVITDLVRREVSSYKQLPLNLYQIQTKFRDEVRPRFGVMRSREFVMKDA
                     YSFHTTQESLQETYDVMYQVYSNIFTRLGLDFRAVQADTGSIGGSASHEFQVLASSGE
                     DDVVFSTESDFAANIELAEAVAVGERQAPTAEMQLVDTPNAKTINELVENHGLAIEKT
                     VKTLVVKGATEEQPLIALVIRGDHELNEIKAQKHPLVADPLEFADEAEIKAKIGAGVG
                     SLGVVNMPIPVIIDRSVALMSDFGCGANIDGKHYFNVNWERDVAVPEIADLRNVVEGD
                     PSPDGKGTLQIKRGIEVGHIFQLGKKYSEAMKATVQGEDGKPLVMTMGCYGIGVTRVV
                     AAAIEQHYDDRGIIWPCDEMAPFTVAIVPMNMHKSEKVQAFAEELYANLKAQGVEVIF
                     DDRKERPGVMFADMELIGVPHMVVIGEKNLENGEIEYKNRRSGEKQMIAKESLLAFLK
                     ANVKA"
     gene            complement(1925..2086)
                     /locus_tag="PXB27_002321"
     CDS             complement(1925..2086)
                     /locus_tag="PXB27_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347063.1"
                     /translation="MEQKLNNVFGGIMFKEKDYDEYLEAKVKKGQIFTLEQANAEWQE
                     IIEQIEREN"
     gene            complement(2139..4022)
                     /gene="htpG"
                     /locus_tag="PXB27_002322"
     CDS             complement(2139..4022)
                     /gene="htpG"
                     /locus_tag="PXB27_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009874843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HDL5347064.1"
                     /translation="MANNQETRGFQSEVKQLLQLMIHSLYSNKEIFLRELISNASDAA
                     DKLRFKALSEPALYEGDGELRVRVSVDETLGTITIADNGIGMTREQVIDHLGTIAKSG
                     TKEFLNALGTDQAKDSQLIGQFGVGFYSSFIVADKVTVKTRAAGVPAEQGVIWESAGE
                     GDYTVADLEKKERGTEIILHLREDEKEFLNEWRLREIIGKYSDHIGLAVEIQTNEYDD
                     EGKASGQKWEKINKAQALWTRSKNEISDEEYTEFYKHLSHDFADPLIWSHNKVEGKQE
                     YTSLLYVPSKAPWDLFQREQKHGLKLYVQRVFIMDDAEVFMPNYLRFMRGLLDTNDLP
                     LNVSREILQENKVTQSLRSALTKRALQLLEKLAKDDAEKYAQFWQAFGVVLKEGVGED
                     FANKQQVAGLLRFASTQRDDSEQAVSFADYIARMKEGQKAIYFLTADSYQAAKNSPHL
                     ELFHKKGIEVLLLSDRIDEWLVSHLTEFDGKPLQSITKSDLDLGELADKQEEESQKAQ
                     QAEFGSFLERAKGYFGERVKNVVLTHRLTDTPAVVSTDSDEMTTQMAKLFAAMGQQAP
                     EVKYTFELNPDHPMVKKVADLADEAEFADWIELLFEQALLAERGTLENPTAFIKRVNK
                     LLG"
BASE COUNT         1014 a         1021 c          839 g         1236 t
ORIGIN      
        1 tatattaaag ggcgtatttt atacgcccaa ttttgttatt tataattaag cctttacatt
       61 cgcttttaag aacgcaagta agctctcttt ggcgatcatc tgtttttcac cgctgcggcg
      121 gtttttgtat tcgatttcgc cgttctcgag gtttttctca ccaataacca ccatatgcgg
      181 cacaccgata agctccatat ccgcgaacat cacgcccggg cgttctttac gatcatcaaa
      241 aatcacctca acgccttgag cttttaagtt agcgtataac tcttcagcga atgcctgcac
      301 tttttccgat ttgtgcatat tcatcggcac aatcgccacc gtaaatggcg ccatttcatc
      361 acaaggccag ataataccac gatcatcata atgttgttca atcgcagctg ccaccacacg
      421 ggtaacgccg ataccgtaac aacccattgt catcactagc ggtttaccgt cttcaccttg
      481 cacagtggct ttcattgctt ctgagtattt tttgcctaat tggaaaatat gccctacttc
      541 aatacctcgt ttgatttgaa gtgtgccttt accgtccggg cttgggtcgc cttccaccac
      601 attgcgtaaa tctgcaattt ccggcacagc aacatcacgc tcccagttca cgttaaagta
      661 gtgtttgcca tcaatattcg caccgcagcc aaaatctgac atcaacgcca ctgagcggtc
      721 aataatcacc ggaatcggca tattcaccac acctaacgag ccaacaccgg caccgatttt
      781 ggctttaatt tccgcttcat cggcaaactc aagcggatca gccaccaacg ggtgtttttg
      841 agctttaatt tcgtttaact cgtggtcgcc acgaatcact aacgcaatta acggttgctc
      901 ttcagttgcc cctttcacca ctaaggtttt caccgttttt tcaatggcta aaccgtgatt
      961 ttctactaat tcattgatgg ttttcgcatt tggcgtatcc actaattgca tttctgccgt
     1021 tggtgcttgg cgttcaccca ccgcaaccgc ttcggctaat tcaatattgg cagcaaaatc
     1081 tgattcggtt gaaaatacca catcatcttc accgcttgag gctaacactt ggaactcgtg
     1141 agaggctgaa ccgccgatag agccggtatc tgcctgcacc gcacggaaat ctaagcccaa
     1201 acgggtgaaa atattgctgt aaacctgata catcacatca taggtttctt gcaggctctc
     1261 ttgtgtggtg tggaaagaat aggcatcttt catcacaaat tcacgcgaac gcattacccc
     1321 gaaacgaggg cggacttcat cacggaattt cgtttgaatt tggtataaat tcagcggaag
     1381 ttgtttgtac gaagaaactt cacggcgaac caagtcggta atcacttctt cgtgagtcgg
     1441 gccaagcacg aaatcacgct cgccacgatc tttaaaacgt aataattccg ggccgtaatc
     1501 attccaacga ccggattcca cccataattc cgccggctga accaccggca ttaatacctc
     1561 gatagccccg cctttgttca tctcttcacg aataatattt tccacttttt tcaacacttt
     1621 gatcccggtc ggcaaccagt tgtataaacc tgacgccatc gggcgaatca ttccggcacg
     1681 caacattaat tgatggctaa ctacttgtgc atcattcggc atttctttaa gggttgaaaa
     1741 taaatattga cttgtacgca ttgctattcc tattcttttt aaactttgaa acctgaaaaa
     1801 atctgcttat tttaaaacaa agcggttaaa ttttcaggaa attttgcaaa atcctataaa
     1861 aaaataaccg cttgtaagcg gttattttgt tttaactaag ggtattttct taaactgtgt
     1921 atgcttaatt ttctcgttca atttgctcaa taatctcttg ccattcggca tttgcttgtt
     1981 caagagtaaa aatctgacct ttttttactt tagcctctaa atattcatca taatcttttt
     2041 ctttaaacat tattcccccg aatacattgt ttaatttttg ttccattata tattgaatat
     2101 ggaaaaacgg aaacctagat ttccgttttt agtaatggct agcccaacaa tttatttacc
     2161 cgtttaataa aggctgtcgg gttttccaac gtgcctcgtt cagcaagcag agcttgttca
     2221 aacagcagtt caatccaatc ggcgaactcg gcttcatcgg caagatcagc tacttttttc
     2281 accattgggt gatctgggtt cagctcaaaa gtatatttca cttctggtgc ttgttgcccc
     2341 attgctgcaa acagttttgc catttgggtg gtcatttcat cgctgtcagt ggaaaccact
     2401 gccggtgtgt cggttaagcg gtgagtcagc accacgtttt tgacccgttc accaaagtag
     2461 cctttcgccc gttctaagaa cgaaccaaac tccgcctgtt gggctttttg gctctcttct
     2521 tcctgcttgt cggcaagctc gcctaagtct aagtccgact tggtaatgct ttgtagcggt
     2581 ttgccgtcaa actcggtgag gtggctaacc aaccattcat caatgcggtc agaaagcagt
     2641 agcacctcaa tgcctttttt gtggaacagc tctaagtgcg ggctgttttt agccgcttgg
     2701 tagctgtctg cggttaagaa gtagatcgct ttctgcccct ctttcattct tgcaatataa
     2761 tcggcaaaac tgaccgcttg ttcgctgtca tcacgctggg tggaggcaaa acggagtaag
     2821 cctgcaacct gttgtttgtt ggcaaagtct tcgcccacgc cctctttaag caccacgccg
     2881 aacgcttgcc agaattgggc atatttttcg gcatcgtctt tcgccaattt ttccagcagt
     2941 tgtaacgcac gcttggttaa ggcagaacgg agcgattggg taactttatt ctcttgcaga
     3001 atttcacggc tgacgttgag cggcaggtcg ttggtgtcga gcaagccacg cataaaacgc
     3061 aagtagttcg gcataaagac ttccgcatcg tccataataa atacacgctg aacgtagagt
     3121 ttcaagccgt gtttttgctc gcgttggaac agatcccacg gggctttgct cggcacatac
     3181 agcaagctgg tgtattcttg cttaccttcg actttattgt gcgaccaaat cagcgggtcg
     3241 gcaaaatcat ggctgaggtg tttgtaaaat tcggtgtact cctcgtcact aatctcattt
     3301 tttgagcgtg tccaaagcgc ttgagctttg ttaatttttt cccatttttg accgcttgct
     3361 ttgccctcgt catcgtattc attggtctga atttccaccg ccaggccgat atggtcggag
     3421 tatttgccga taatctcacg caaacgccat tcgtttaaaa actctttttc gtcctcacgc
     3481 aagtgaagaa tgatttccgt gccacgctct tttttctcca gatcggcaac ggtgtaatcg
     3541 ccctcaccgg cagattccca aatcacgcct tgctcggcag gcactccggc ggcacgggtt
     3601 ttgactgtca ctttatccgc cacgatgaaa gaagagtaaa agcccacgcc gaactgcccg
     3661 ataagctggc tgtctttggc ttgatcagtg ccgagtgcgt tcaaaaattc tttggtacca
     3721 gattttgcaa tcgtaccgag atggtcgatc acctgctcac gggtcatacc gataccgtta
     3781 tcggcaatcg taatcgtacc gagtgtttca tcaacagaga cacgcacacg cagttcgcca
     3841 tcaccttcat acaaagccgg ctcggaaagg gctttaaacc gcaatttatc ggcggcgtca
     3901 gaggcgttgg aaataagctc acgcaggaaa atttctttgt tggagtaaag cgagtggatc
     3961 atcagttgca ataactgttt cacttccgac tggaagccac gggtttcttg attatttgcc
     4021 attgtgtatt cctttattca ttaaaaaaca gggaatagat ggggcaagtg gtcagatttt
     4081 caagattttt tgcaaaatat ctgttgccaa
//
LOCUS       DANLUQ010000177         4020 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.178, whole genome shotgun sequence.
ACCESSION   DANLUQ010000177 DANLUQ010000000
VERSION     DANLUQ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.178"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(123..1763)
                     /gene="pgi"
                     /locus_tag="PXB27_002323"
     CDS             complement(123..1763)
                     /gene="pgi"
                     /locus_tag="PXB27_002323"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013527751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDL5347065.1"
                     /translation="MQKINPVQTFAWNALEQHKADNLSIPQLFAEDPNRFDKYSLRFE
                     DQLLVDFSKNAINEKTLTLLRQLANECGLESAKNAMFSGKKINRTENRAVLHVALRNR
                     ANTPIAVDGKDVMPEVNAVLAKMKDFCERVISGEWKGYTGKTITDVINIGIGGSDLGP
                     YMVTEALRPYKNHLNMHFVSNVDGTHIAEVLRKVNPETTLVLVASKTFTTQETMTNAL
                     SARKWLLDSAKDESAVAKHFVALSTNAKEVEKFGIDTANMFEFWDWVGGRYSLWSAIG
                     LSIALSLGFENFEQLLAGAHAMDKHFLITPIEKNIPTTLALVGIWNNNFLGAESEAIL
                     PYDQYMHRFAAYFQQGNMESNGKFVGRDGKPVTHQTGPIIWGEPGTNGQHAFYQLIHQ
                     GTKLIPCDFIAPAQTHNPLSDHHNKLLSNFFAQTEALAFGKSKETVEKEFLDAGKTLE
                     EVAEIVPFKVFSGNKPTNSILVQKITPYSLGALIAMYEHKIFVQGVLFNIYSFDQWGV
                     ELGKQLANRILPELETDEKINSHDSSTNGLINQFKAWR"
     gene            complement(1796..2044)
                     /locus_tag="PXB27_002324"
     CDS             complement(1796..2044)
                     /locus_tag="PXB27_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HDL5347066.1"
                     /translation="MLTPKNLEAIAQQLHNALPQSLKNVGNDLDEKFKQILQAQLARL
                     DVVTREEFDVQSQVLLRTREKLTELEKRLDELTQRTDE"
     regulatory      2168..2212
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            2216..2884
                     /gene="queC"
                     /locus_tag="PXB27_002325"
     CDS             2216..2884
                     /gene="queC"
                     /locus_tag="PXB27_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HDL5347067.1"
                     /translation="MTHQKPKAVVIFSGGQDSTTCLFQAIAEFGKENVEVVTFQYGQR
                     HSIELEKARWISNDLGVKQTLIDTSVIKAITTNALMDNNAEIQQQGSTPNTFVDGRNA
                     LFLLYTAIYAKGQGIQTIFTGVCETDFSGYPDCRDVFVKSMNVTLNLAMDYNFNIRTP
                     LMYLTKKETWALADKLGAFDYIRDHTHTCYLGVEGGCHDCPSCVLREKGLNEYLAERE
                     VSNV"
     gene            2877..3299
                     /gene="queD"
                     /locus_tag="PXB27_002326"
     CDS             2877..3299
                     /gene="queD"
                     /locus_tag="PXB27_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005597968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HDL5347068.1"
                     /translation="MFKIAKEFSFDMAHMLDGHDGKCQNLHGHTYKLQVEVAGELFQE
                     GAKRGMVMDYSDLKAVVKQHILEPMDHAFIYDLQSERESQVAQLLIDLNSKVYGIPTR
                     TTAEEMAKYMFEKLEKVGLPVSLIRLWETPTSYCEYSK"
     gene            3299..3925
                     /locus_tag="PXB27_002327"
     CDS             3299..3925
                     /locus_tag="PXB27_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HDL5347069.1"
                     /translation="MAELISNPRFPIVEIFESLQGEGFNTGMPAIFVRFGKCNLTCPW
                     CDTNYNQFEQWTLSDILAKVRSYSAKNVIITGGEPTIQPNLSLLLEQFKKEGYFLAIE
                     TNGLKEVPAQIDYIATSPKRMYQEKYQRRCIEFANEVRIVVDGEVQGFCEQIESQIKA
                     EHYYLSPCEVEGKMNLLETITQIGLLNQRPNKPKWQLSIQTHKIVGIE"
BASE COUNT         1208 a          749 c          854 g         1209 t
ORIGIN      
        1 gcaaaagaat atagaaaaaa ctgcttgtgt agaaatggcg cttagttcag tcctcagcta
       61 aaattttaga aaaaaagcat agagattttt tacctctatg ctttttattg tattgactat
      121 atttaacgcc aagccttgaa ttggttaatt aaaccatttg tggagctgtc gtggctgttg
      181 attttttcat cggtttcgag ttcgggtaaa atgcggtttg caagctgttt gcctaactcc
      241 acgccccatt ggtcgaaact gtaaatgttg aacagcacgc cttgcacgaa gattttgtgt
      301 tcatacattg caattaatgc ccctaagctg tacggggtga ttttttgcac caagatagag
      361 ttggtcggtt tgtttccgct gaacacttta aacggcacga tttcagctac ttcttccaac
      421 gttttacctg catctaaaaa ctctttttca actgtttctt ttgatttacc gaaagctagt
      481 gcttcagttt gagcaaagaa gttagaaagc agtttgttgt gatgatcgct taacggattg
      541 tgagtttgtg ccggtgcgat aaaatcacaa ggaattaatt ttgtgccttg atgaatgagt
      601 tgatagaatg cgtgctgacc gttagttccc ggttcgcccc aaataatcgg acctgtttgg
      661 tgagtaaccg gtttgccgtc acgccccaca aatttaccgt ttgattccat attgccttgt
      721 tggaaataag ccgcaaagcg gtgcatatat tggtcgtaag gtaaaatcgc ttctgattca
      781 gcacctaaga aattgttatt ccaaatgccg actaaagcta acgtagttgg aatgtttttc
      841 tcaatcggcg tgattaagaa atgcttatcc attgcgtgag caccagcaag tagctgctcg
      901 aagttctcaa aacctaatga aagggcaatc gaaagcccga tggcagacca taatgagtaa
      961 cgaccgccaa cccaatccca gaattcaaac atattagcgg tatcaatacc gaatttttcc
     1021 acttctttgg cattggttga aagtgccacg aaatgttttg ctaccgcaga ttcatctttg
     1081 gcagaatcaa gtagccattt gcgagctgaa agggcattgg tcatcgtttc ttgagtggta
     1141 aaggtttttg aagccactaa cactaacgtg gtttcaggat taactttacg caacacttca
     1201 gcaatatgcg tgccgtccac gttagacaca aagtgcatat taaggtgatt tttataaggg
     1261 cgtaatgcct ctgttaccat ataaggacct agatccgagc caccaatacc gatattgatc
     1321 acatcagtaa tcgttttgcc ggtatagcct ttccattcgc cggaaataac acgctcgcag
     1381 aaatctttca ttttcgctaa cacggcattc acttccggca ttacatcttt gccatcgact
     1441 gcgattggtg tattggcacg gttacgcagt gcaacgtgca atacggcacg gttttcagtg
     1501 cggttgattt ttttgccgga gaacatcgca ttttttgctg attccaaacc acattcattt
     1561 gccagttggc gaagtaaggt taaggttttt tcgttgatcg cattttttga aaaatcgact
     1621 aataattgat cttcaaaacg gagggaatat ttgtcgaaac ggtttggatc ttcggcaaaa
     1681 agttgaggaa tagaaaggtt gtctgcttta tgttgctcta aggcattcca agcaaaagtt
     1741 tgcacagggt taattttttg catctgagtt tccttatact aaattaaaaa atcgattatt
     1801 catctgttct ttgagtcagt tcatcaagac gtttttctaa ttcggttaat ttttcacgag
     1861 tgcggagtaa cacttgagat tgaacatcaa attcttcacg agtgacaaca tcaagacggg
     1921 ctaattgggc ttgaaggatt tgcttaaatt tttcatctag atcattaccg acatttttta
     1981 agctttgcgg taaggcgtta tggagttgtt gagcaatcgc ttctaagttc ttcggcgtga
     2041 gcatatttaa attccttact ataaatgagt atcccaattt tattgaattt tgggtttaag
     2101 agaaagcaaa ttctttgtaa aatatcacct ttcgcaaaat acaagcaaaa aagaccgctt
     2161 gtatttttta tttgtggttc gtaaacctcc cacacaaaaa aactaaggac acaaaatgac
     2221 tcatcaaaaa ccaaaagccg ttgtgatttt ttccggcggt caagactcta ccacttgttt
     2281 attccaagct attgctgaat ttggcaaaga aaatgtggaa gtcgttacct ttcaatatgg
     2341 gcaacgccat tcgattgaac ttgaaaaagc acgctggatt tctaacgatt taggcgtcaa
     2401 acaaacgctg attgatactt cggtgattaa agcgattact accaatgcgt taatggataa
     2461 taatgctgaa attcaacaac aaggcagcac acccaatacc tttgtggatg ggcgtaatgc
     2521 cttatttctg ctttataccg ccatttatgc caaagggcag ggcattcaaa ctatttttac
     2581 cggcgtgtgt gagaccgatt ttagtggtta ccctgattgc cgagatgtat ttgtaaaatc
     2641 aatgaatgta acgctaaatt tagcgatgga ctataatttc aacattcgca cgccattgat
     2701 gtatttaact aaaaaagaaa cttgggcatt agcagataag ctgggtgctt ttgattatat
     2761 tcgagaccat acccacacct gttatttagg cgttgaaggt ggttgccacg actgcccgag
     2821 ttgcgtattg cgagaaaaag gcttgaatga atatttagca gaaagggagg taagcaatgt
     2881 ttaaaattgc taaagaattt agttttgata tggctcatat gctagatgga cacgatggca
     2941 aatgccaaaa cttacacgga cacacctaca aattgcaagt tgaggtggca ggtgagctat
     3001 ttcaagaggg agctaaaaga ggaatggtaa tggactattc ggatctaaaa gcagtggtaa
     3061 aacaacatat tttagagccg atggatcacg cttttattta cgacttacag agtgagcgtg
     3121 aaagccaagt agcacaatta ttaattgatt taaactccaa ggtttatggt atcccaactc
     3181 gtaccacagc cgaagaaatg gcgaaatata tgtttgaaaa attagagaaa gtgggcttac
     3241 cggttagcct aattcgcttg tgggaaacgc ctacttctta ttgtgagtac agcaaataat
     3301 ggcggagtta atttcaaacc ctcgttttcc gattgttgag atctttgaaa gcttacaagg
     3361 ggaaggcttt aataccggaa tgccggctat ttttgtacgt tttggcaaat gcaacctcac
     3421 ttgcccgtgg tgcgatacca attacaatca atttgagcaa tggacgttaa gcgatattct
     3481 tgccaaagta agaagctatt ctgcgaaaaa tgtgattatt accggaggcg agccaacgat
     3541 tcagcctaat ttatcgttat tattagaaca atttaaaaaa gagggatatt ttttagcgat
     3601 cgaaaccaac ggcttaaaag aggttccggc acaaattgat tatattgcga ccagcccaaa
     3661 gcgaatgtac caagaaaagt atcaacgccg ttgcattgaa tttgcgaatg aagtccgtat
     3721 cgtcgttgat ggagaagtac agggtttctg tgagcaaata gaaagccaaa ttaaagctga
     3781 gcattactat ctttcacctt gtgaggtaga gggaaaaatg aacttgctgg aaaccatcac
     3841 ccagattggg ttattaaatc agcgtccaaa taagcctaaa tggcagctaa gtatccaaac
     3901 gcataaaatt gtggggattg aataagtaga agttagaaga tgatttgtaa aagatcagac
     3961 ttgatctgac aattcactct aaaaagtgag agtttcccgt ttagaatatg agtgtccaaa
//
LOCUS       DANLUQ010000178         4001 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.179, whole genome shotgun sequence.
ACCESSION   DANLUQ010000178 DANLUQ010000000
VERSION     DANLUQ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.179"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(156..2720)
                     /locus_tag="PXB27_002328"
     CDS             complement(156..2720)
                     /locus_tag="PXB27_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HDL5347070.1"
                     /translation="MRIAKIIFSALLSLLVLGGVIGGLLYMHFKSDLPDVATLKNVEL
                     QQPMQIFTLDGKLIGEVGEERRIPVKLDDIPPMLVKAILATEDARFYEHKGIDPKGIM
                     RAIWRTSQGDTQGASTITQQLAKNFFLTPERSLERKAKEAILALEIEQVLSKNEILEL
                     YLNKIYLGYRSHGVAAAAKTYFNKSLKDLTLSEIAIIAGLPKAPSTMNPLYSVKRAED
                     RRNVVLGRMLETGDITKEEYEKAKAEPVVANYYGATLEFRADYVTEMVRQEIVKRYGE
                     NIAYNKGLKVYATVLSADQKAAQDALRENLIDYDRRHGWRGAEKLWTGSSAWDDEKII
                     DHLSKLPKSEPFTPAVVMQADKSKYTILLANGDISVLKRTNASFAPKGLKAGEQIWVR
                     QNKNREWALGQIPEVNSALVSINSENGAIESIVGGFSFEQSRFNRATQSLVQVGSAIK
                     PFIYTAAMNKGLSLSTTISDSPIVIKKKGQKEWRPKNADGVYGGPLRTRVALGKSKNM
                     VAIRILQMAGIDYVADYLQRFGFNRNQYVATESLALGAASFTPLEMARAYAVFNNGGY
                     LIEPYIIDRIIDAQGYELYQANPVVACNSCDSPVVYPEPKYFESVKIHDEAQVGVQTI
                     ANSSNNDDDDVVEDVGDSEPELQANNSEKNAPSLMAESSHHIKGLRYAPHVISDELAF
                     LMRSALATAITGEPEYGWRGTSYKMLNTIKRADVGGKTGTTNNAKATWYAGFGANIST
                     VVYVGFDDNKRVLGRGASGSSTALPAWTNYMKVALSDKPVEKSTLPKNIIEVNIDPNS
                     GFLGNGRKEYFIKGTEPTKRYIVERAYTEKAPQKTESAPLRLGLPPPGVLKSGELF"
     gene            2844..3542
                     /locus_tag="PXB27_002329"
     CDS             2844..3542
                     /locus_tag="PXB27_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComA"
                     /protein_id="HDL5347071.1"
                     /translation="MKLLKAHSKTPSNRIILGLSEDEQRYCLVKNKAEKFTVFWQAKP
                     ASIEELIGNLTPQEQAMMTLVRPIPYQYIWRKTVFMANTLNEAQLHRKVIHILKNEQP
                     LALDLLNIDYQRFPSSNDNLDKVVIYAVRKSYTENLSQFNSILDCELHCYARAIIHLT
                     QSQNNQPAPCFSFKQKYFQFTETELLILQTEPEDCIHLSDLKIDHLEIECEQEKQLYC
                     LALGASLWNGKVLI"
     gene            3518..>4001
                     /locus_tag="PXB27_002330"
     CDS             3518..>4001
                     /locus_tag="PXB27_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347072.1"
                     /translation="MEWQGIDLSANRKNNWFAGSDKGIKNVIYTLLLSALISLALIFL
                     YLQKNQEIQPLAQQLELVKTDILNIENKIISLNSNHTNTLPFAEPKKIAQLLAIIDNL
                     PLKNGGIETIQIYLETDLYLKIIGNLDNQSDFQSLESYLYQQDLFETKTEQINVNHKN
                     E"
BASE COUNT         1255 a          865 c          649 g         1232 t
ORIGIN      
        1 tctgatttct ttaaaaaatt gcaataaaac tcatttattg actaaggaat atccaaatat
       61 aactcacaaa tcgcccaaaa taaaattggc acttattatc gcaagaaatc cccctatttt
      121 tcaactttta agagcattat ccttgtaata aattattaga ataattcacc gctttttagc
      181 acccctggtg gaggtaatcc taaacgtaac ggcgcagatt ctgttttttg aggcgctttt
      241 tcagtataag ctctctctac aatataacgt tttgtaggct cagtaccctt aataaaatac
      301 tctttacgtc catttcctaa gaaaccacta ttagggtcaa tattaacctc aataatattt
      361 tttggaaggg tacttttttc gacaggctta tctgacaatg caactttcat ataatttgtc
      421 caagctggta aagcagtact agaacctgaa gcacctctac ctaatactct tttattatca
      481 tcaaagccaa cgtacacaac agtagaaata ttagcaccaa atcccgcata ccaagttgcc
      541 ttggcattat tggttgtacc agtcttcccg cccacatccg ctcgttttat cgtattgagc
      601 attttatagc ttgttcctcg ccagccatat tcaggctcac cagtaattgc tgtagctaaa
      661 gcactacgca ttaaaaaagc gagttcatca ctaataacat gaggagcgta acgtaaccct
      721 ttgatatgat gagaagattc tgccattaag ctcggcgcat ttttctcgct attgttcgct
      781 tgtaattcag gttctgaatc gccaacatct tccacaacat cgtcgtcatc attattcgag
      841 gaattcgcta tagtttgtac accgacttga gcttcatcat gaattttgac tgattcaaaa
      901 tatttaggct cagggtaaac aactgggcta tcgcaactat tacaagctac tacaggattt
      961 gcttgatata actcgtagcc ttgggcatca ataatacggt caatgatata tggttcaatc
     1021 aaataacctc cattattaaa tactgcataa gctcgagcca tttctaatgg cgtaaaagat
     1081 gcagcaccca atgcaagaga ttctgttgca acatactgat ttcggttaaa gccaaaacgt
     1141 tgtaagtagt ctgcaacata atcaatccct gccatttgta atatacgaat agccaccata
     1201 tttttcgatt tacctaatgc aacacgagtt ctcaacgggc cgccataaac cccatcggca
     1261 tttttaggtc gccactcttt ttgccctttc tttttaatga caataggaga atcgctaatt
     1321 gttgttgaaa gacttagccc tttattcatt gccgctgtgt aaataaacgg tttaatcgca
     1381 gagcctacct gaactaatga ctgagtcgca cgattgaaac ggctttgttc aaagctaaag
     1441 cctccaacga tagattcaat ggcaccgttt tcactattaa ttgacactag ggctgaattt
     1501 acctccggaa tttgtcctaa agcccactcc ctatttttat tttgacgtac ccaaatttgt
     1561 tcacctgctt ttaacccttt aggtgcaaaa gaagcatttg tacgttttaa aacagagata
     1621 tcaccatttg ccaataaaat tgtatatttt gatttatctg cttgcattac aacagctggt
     1681 gtaaacggct ctgattttgg cagcttactt aaatgatcga taattttctc atcgtcccaa
     1741 gcagaactac ctgtccataa tttttcagcg cctcgccaac catgacggcg gtcataatca
     1801 atcaaatttt cacgtaacgc atcttgtgct gctttttggt ctgctgaaag taccgtggcg
     1861 tacactttta agcctttgtt ataagcaata ttttctccat agcgtttcac aatttcttga
     1921 cgaaccattt ctgtcacata atccgctcga aattctaatg tcgcaccata gtagttagct
     1981 actactggct ctgctttagc tttctcatat tcctcttttg taatatctcc ggtttctagc
     2041 attctcccaa gcactacatt acgacggtct tccgcccgtt ttactgaata aagtggattc
     2101 atcgttgaag gcgcttttgg aagccctgca ataattgcta tttcagaaag ggttaaatct
     2161 ttcaaagatt tattaaaata agttttagcc gcagcggcaa caccgtgaga acgataccct
     2221 aggtaaattt tgtttaaata cagctccaga atctcatttt tactgagaac ctgttcaatt
     2281 tctaaagcta aaattgcctc tttggcctta cgctctaaac tacgttcagg tgttaagaaa
     2341 aagtttttcg ccagctgctg ggtgattgta cttgcgcctt gagtatctcc ctggctagtg
     2401 cgccatattg cacgcataat acctttagga tcgatccctt tatgctcgta aaaacgtgca
     2461 tcttcggtag caagaatcgc ttttactaac atcggtggaa tatcatcaag cttgaccgga
     2521 atacggcgtt cttctcctac ttcaccaatt aatttaccat ctaaggtaaa aatctgcata
     2581 ggctgctgta actctacatt tttcagcgtt gccacatcag gcaaatcaga tttgaaatgc
     2641 atgtacagta aaccaccaat taccccacct aaaaccaaga gtgaaagtaa agcactaaat
     2701 attatttttg cgatccgcat cgaaaaaatc tcttttggtt aatgacgaaa cataaattta
     2761 aagccaatat taggtcacta agtataagct aaaaacgctt acttcgcata actaattgca
     2821 aaactttcct tactatactt actatgaaat tacttaaagc acattcaaaa acaccttcaa
     2881 accgtatcat cttaggctta agtgaagatg aacaacgtta ctgcttagtc aaaaataaag
     2941 cagaaaaatt caccgttttt tggcaagcca agcctgcgag catcgaagag cttatcggta
     3001 atctcacccc acaagaacaa gcaatgatga cgctggttcg cccgatccct taccaataca
     3061 tttggcgaaa aaccgttttt atggctaaca ccctcaatga agctcaatta caccgtaagg
     3121 taattcacat tttaaaaaat gagcagccac ttgcattgga tctactcaat attgactatc
     3181 agcgatttcc aagcagcaac gataatctgg ataaagtggt tatctacgcc gtgcgaaaaa
     3241 gttataccga gaatttgagc caatttaaca gcatcttaga ttgtgagctg cattgctatg
     3301 cacgggctat aattcattta acacaatccc aaaataatca gcctgcgccc tgtttttcat
     3361 ttaaacagaa gtacttccaa tttaccgaga cggaattact gatattgcaa acagaacctg
     3421 aggattgtat tcatctatct gatctcaaaa ttgaccatct tgaaatcgag tgtgagcaag
     3481 agaaacagct atattgcctc gccttaggag caagtttatg gaatggcaag gtattgattt
     3541 aagtgctaat cgaaaaaata actggtttgc cggcagtgat aaaggcatca aaaacgtgat
     3601 ttatacactt ttattatctg ccctcattag cttggctctg atttttctct atttgcaaaa
     3661 aaatcaagaa attcaaccgc ttgcacagca attagagctg gtaaaaaccg atatattaaa
     3721 tattgaaaat aaaattatca gcttgaatag taatcataca aacaccttgc cctttgccga
     3781 gcctaaaaaa atagcacaac tactggcaat aatagataac ttaccgctca aaaatggggg
     3841 aattgaaacg atacaaattt atcttgaaac cgatctctat ttaaaaataa tcggtaattt
     3901 agataatcaa tctgattttc aaagtctaga atcttattta tatcagcaag atttatttga
     3961 aaccaaaacc gagcaaatta atgttaatca taaaaatgaa a
//
LOCUS       DANLUQ010000179         3918 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.180, whole genome shotgun sequence.
ACCESSION   DANLUQ010000179 DANLUQ010000000
VERSION     DANLUQ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.180"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(303..1397)
                     /locus_tag="PXB27_002331"
     CDS             complement(303..1397)
                     /locus_tag="PXB27_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HDL5347073.1"
                     /translation="MKKTTLAILASALIANSALAITIYDHKETGTQIEFIGSARIKWE
                     STSDKTSPVNGKATRNHVNHAVDNNGSRFGFRLTQQVGHGFYALGRVEWRMRGDSSSQ
                     HNFDHIYDHQLYAGIGHKQYGELTYGNMTNIADEVKQTDLPNTLSLSDGLLTGSSRRT
                     LQYVYKGIEGLKLGAFYGGNSQRGNNGLDLVNKRKDIWGAAAIYNYKIDDNQSLKLAT
                     GATRERSEQSIGNYERIALAFGTAYTYGKTTLGLDLERRETEDQNAIGHKRIEKEVRT
                     VLLHRLTDQWNAYTMYAYKENKLNAVGLANDSKVKRNQFMLGTEYYIVPKHLKGFVEW
                     QATRAKHYTNDVKTAKSRNYTTVVGLRAYW"
     gene            1531..2709
                     /gene="lpxB"
                     /locus_tag="PXB27_002332"
     CDS             1531..2709
                     /gene="lpxB"
                     /locus_tag="PXB27_002332"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HDL5347074.1"
                     /translation="MKQQFPVIGLVAGEVSGDILGAGLIKALKVHYPNARFIGVAGSR
                     MIEAGCQSLFDMEELSVMGLAEVVKHLPRLLKRRKQVIDEMLKLKPDVFIGIDAPDFN
                     LTVEEKLKASGIKTIHYVSPSVWAWRQKRVFKIARAANLVLAFLPFEKAFYDKFNVPC
                     RFIGHTMADAIPLEPNRTEACLALNIDESKRYMAILVGSRGSEIQFLAEPFLKAAQLL
                     KARFPDLQFLVPMVSQKRMEQFNAIKQQVAPELELNIIQGKARQAMIASECTLLASGT
                     AALEAMLCKSPMVVGYRMKPLTYWLAKKLVKTDYISLPNLLAQAPLVPELIQEACNPE
                     NLAQELSLFLADDEKNQQHKALLKQQFMQLHQSIQCNADEQAAQAVIDLLNGSTSGQI
                     "
     gene            2736..3788
                     /gene="sohB"
                     /locus_tag="PXB27_002333"
     CDS             2736..3788
                     /gene="sohB"
                     /locus_tag="PXB27_002333"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005595593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HDL5347075.1"
                     /translation="MWKEVLLNYGIFLLELLTVFGIIAVVVMLILESKKQNENGSISI
                     TNLSEKYKEQQKSLSGFFLSEEELKHQEKQEKKVAKEKAKAEKKRLKAGEECVESPSR
                     LFVLNFNGDMMAHEVNSLRREIDAVISLANPEKDEVLLKLESPGGVVHGYGLAASQLQ
                     RLRDRNIYLTAAVDKVAASGGYMMACVANKIVSAPFAVIGSVGVVAQVPNIHRLLKKH
                     DIDVDVMTAGEYKRTVTLVGENTEKGKQKFQQELEETHSLFKQFVARHRPQLDIEQVA
                     TGEHWFGQQAIELNLVDEIATSDDLLLNAIKEKEVIELKYKEKKKLAQKLGSQFEQSA
                     ENLLAKILHKSRSTLM"
BASE COUNT         1165 a          676 c          850 g         1227 t
ORIGIN      
        1 taataacccc tcatcacttt ttgcagttta acctcttttg caacagataa tttaaattta
       61 ggcgttttgg ctgctcagtt attgccatta tgattatatt agttcacact aaaattttag
      121 tgttgtttct taaagctgaa gatgctaata ggtgagtaag cggtttttac cactttgaag
      181 ataaatgcaa tagcttcttg tttgaaggat tagttgtatt taatcataaa aaatctgcac
      241 cttaatcagt tggttagccc aacttttagg tgcagatcat tctagtacgc ttttcagttt
      301 tattaccaat atgctcgtaa gcctacaaca gtcgtataat tgcgagattt tgcggttttt
      361 acatcattag tatagtgttt agcacgagtt gcttgccatt caacaaagcc ttttaagtgt
      421 tttggaacaa tgtaatattc cgttcccaac ataaattggt ttcttttaac tttgctatca
      481 tttgccaagc ctacagcatt aagtttgttt tctttataag catacattgt gtaagcattc
      541 cattgatcgg ttaaacgatg gagtaacaca gtgcgaactt ctttttcgat acgtttgtga
      601 ccgatagcat tttgatcttc cgtttcacgg cgttctaaat ctaagccaag ggtagttttt
      661 ccgtaggtat aagctgtacc gaaggcaaga gcaatgcgtt cataattgcc gatagattgc
      721 tcagaacgct cacgggttgc acctgttgct aattttaagc tttggttatc atcaatttta
      781 tagttataaa ttgctgctgc accccaaata tctttacgtt tattgactaa atctaagcca
      841 ttgtttccac gttggctatt tccgccatag aatgctccga gttttaatcc ttcgatgcct
      901 ttgtaaacat attgaagcgt acgtcgtgat gaaccggtaa gtaagccatc acttaagctg
      961 agtgtatttg gtaagtcagt ttgttttact tcatcggcaa tattagtcat attgccgtat
     1021 gttaattcac cgtattgttt atgcccgatt cctgcataga gttgatgatc ataaatatga
     1081 tcgaagttat gttgtgagct tgaatcgcca cgcatacgcc attccacacg acctaaagca
     1141 tagaatccgt gacccacttg ctgggttaaa cggaaaccaa aacgagagcc gttgttatcc
     1201 actgcgtgat taacgtggtt gcgggtagct ttaccattta ccggtgaggt tttatctgag
     1261 gtgctttccc acttaatacg agcagaacca ataaattcaa tttgtgtacc ggtttcttta
     1321 tgatcgtaaa tcgtaatagc tagagctgag tttgcgatta aggctgatgc taaaatcgct
     1381 aatgttgttt ttttcataaa aatcactcca tatgaataga taaagggagg tagtgtatgt
     1441 gaatattcat tgctagacaa taatgttttg ttagtttata tgatgattgg ttataataga
     1501 gtcacttaca gatcttcaat aatatttaat atgaaacaac aattccccgt tattggctta
     1561 gttgctggtg aggtctctgg cgatattctt ggtgccgggt taattaaggc gttaaaagta
     1621 cattacccga atgctcgatt tatcggtgtt gcaggctctc gaatgattga ggctggctgc
     1681 cagtctttgt ttgatatgga agagctttct gtaatggggc tagcagaggt ggttaaacat
     1741 ttacctcgtt tacttaagcg tcgtaaacag gttattgatg agatgcttaa attgaaacca
     1801 gatgtattta tcggtattga tgcaccggat tttaatttaa ccgttgaaga aaaactcaaa
     1861 gcgagtggga ttaaaacaat ccattatgtt agcccttcgg tgtgggcttg gcgacaaaag
     1921 cgggtgttta aaattgccag agcagctaat ttagtgttgg catttctgcc atttgagaaa
     1981 gcgttctatg ataaattcaa tgtgccttgt cgttttatcg ggcatacgat ggcagatgcg
     2041 atcccactcg aaccaaatcg tacagaggca tgtttagcgt taaatattga tgaatccaag
     2101 cgatatatgg caattttggt gggaagccgt ggcagtgaaa ttcagttttt ggctgagcct
     2161 tttttgaagg ctgctcagtt gttaaaagcc cgttttccgg atttacagtt tttagtgcca
     2221 atggtcagcc aaaagcgaat ggagcaattt aatgcaatta aacagcaagt agctcccgag
     2281 ttggaactga atattattca aggcaaggct cgtcaggcta tgattgcgtc agaatgtact
     2341 cttttggcat cgggaacagc agcactagag gcaatgttgt gcaaatctcc gatggtagtt
     2401 ggttatagaa tgaaaccgct gacttattgg ttagcaaaaa aattagtgaa aaccgattat
     2461 atctccttac cgaatttatt ggctcaagcc ccattggtac cagaattgat ccaagaagca
     2521 tgcaacccgg aaaatttagc tcaagagtta tctctatttt tagctgatga tgagaaaaac
     2581 caacaacata aagcattgct aaaacagcag tttatgcagc tacatcaatc aattcaatgt
     2641 aatgctgatg agcaagctgc ccaagcggtt atcgatctcc taaacggctc gacaagcggt
     2701 caaatttagt tagttttttg taaaaaggaa aatttatgtg gaaagaagta ttacttaatt
     2761 atggtatttt tttattagag ttattaactg tatttggcat tattgcagtg gtggtgatgc
     2821 tgattttgga atcgaaaaag cagaatgaaa acggctcaat ttcaattacc aatttatctg
     2881 agaaatataa agagcagcaa aaatctcttt ccggcttttt tctaagtgag gaagaattaa
     2941 aacatcagga aaagcaggag aaaaaagtag caaaagagaa agctaaagca gaaaaaaaac
     3001 gcttaaaagc aggcgaagaa tgtgtagaaa gcccttcccg tttatttgta ttgaatttta
     3061 atggcgatat gatggctcac gaagtaaatt ccctacgccg tgaaattgat gcggtaatta
     3121 gcctcgcgaa tccggaaaaa gatgaagttt tacttaaact tgaaagtccg ggcggagtgg
     3181 tgcacggtta tggattggct gcttcgcagt tacaacgttt gcgtgatcgc aatatttatt
     3241 taaccgcagc ggtagataaa gtagctgcca gcggtggtta tatgatggcg tgtgtggcga
     3301 ataaaattgt ctctgcccca tttgcggtga ttggctcagt gggggtggtg gctcaggtgc
     3361 cgaatatcca ccgcttactg aaaaaacacg atattgatgt ggatgtgatg actgcaggtg
     3421 aatataaacg cacggtgacg ctggtgggag aaaataccga aaaagggaaa cagaaattcc
     3481 agcaagaact ggaagaaacg cactctctgt ttaagcaatt tgtggctcga caccgcccac
     3541 aactggatat tgagcaggtt gcaacaggcg agcattggtt tggtcagcaa gcgattgagc
     3601 taaatttggt tgatgagatt gcaaccagtg atgatctgct cctcaatgca attaaggaaa
     3661 aagaagtgat cgagctaaaa tataaagaga agaaaaaact tgctcagaaa ttgggttctc
     3721 aattcgagca atcagcagag aacctgctcg ctaagatttt acataaaagt cgttcaacgc
     3781 tgatgtagcg tatatattat taaagggcta tcgtttatat agccccttat tttatcagtt
     3841 agtaatagaa tgaagtattt gttcactatt taaatgacct ggaatacttt tctttaaatt
     3901 gagtgaagaa tctaacaa
//
LOCUS       DANLUQ010000180         3907 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.181, whole genome shotgun sequence.
ACCESSION   DANLUQ010000180 DANLUQ010000000
VERSION     DANLUQ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.181"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..1298)
                     /locus_tag="PXB27_002334"
     CDS             complement(<1..1298)
                     /locus_tag="PXB27_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HDL5347076.1"
                     /translation="MKTLTLTANKITAVEGAAIGANQGSFAQKLNSTKARIDQLNAYR
                     IERAINANNHHFMHVFSLIPLLIHLNHPVLPAYVENAPQGIWHFQLSDYQKQFLVSYR
                     FDEAISADHLGPHPSFDALYSMGSTGTITQTSCSDLDLWLCYPNHFTIEQYKLMELKI
                     NKLQEWAKTFQIDINIYLMNPEQFKSQTYNSSVTDEHSGSAQHFFLLDEFYRSAILLA
                     GKRLLWLHLHKNEYQNVHNNPEIDLTEWIDFGDFSSLSTSEFFGASLWQLYKGINNPY
                     KSAIKILLLECYAHTYPKTKLISKEFKKKLLSDNALEYHFDPYLAMLELVTEHLRSRK
                     EWVKLDRLRVCFYTKAVEGEINNNWRSRDLDELVNQWSWSEKQIQLLQNRQHWKIKQV
                     MNHENMLIDHLLQSYRNLIHFARKFHINPSIMTNDTDILMR"
     gene            1559..2776
                     /gene="fabB"
                     /locus_tag="PXB27_002335"
     CDS             1559..2776
                     /gene="fabB"
                     /locus_tag="PXB27_002335"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HDL5347077.1"
                     /translation="MKRVVITGLGVISSIGNNKEEVLESLKAGKSGIEFVPEFAEVGM
                     RSQVAGTIKLNPAELIDRKIYRFMGDAAAYAYLSMKEAIEDSGLTEEQVSNDRTGLVI
                     GAGTGSAHSQLVACDAVRGPRGVKAVGPYAVTKTMASSVSACLATPYKIRGVNYSISS
                     ACATSAHCIGHAMELIQLGKQDVVFAGGAEELSWECATEFDAMGAVSTKYNETPTKAS
                     RAYDANRDGFVIAGGGAVVVVEELEHALARGAKIYAEIVGYGATSDGYDMVAPSGEGA
                     ERCMRQAMATVNGEIEYINVHGTSTPVGDVKELGAIRNVFGDKTPAISSTKSMTGHSL
                     GAAGAHEAIYSLLMLDNNFIAPSINIETLDEQAQGMNIVTERQDKALTTVMSNSFGFG
                     GTNACLVFQRYSK"
     gene            2894..3040
                     /locus_tag="PXB27_002336"
     CDS             2894..3040
                     /locus_tag="PXB27_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347078.1"
                     /translation="MIKPLDSVYWRGKDVSEFFARKRLPDTAVQCVKDNELSYVYGYS
                     TSFV"
     gene            3009..3719
                     /locus_tag="PXB27_002337"
     CDS             3009..3719
                     /locus_tag="PXB27_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HDL5347079.1"
                     /translation="MFMVIQLALYNLQTYEVGRNATTIFMTTDKVYTGGSTWTYLFAD
                     ADNKITSVREMNGFGDVEKEFKCGEYKEAVTIASKEDLAKRKTQPRLWSSIYMSPEQN
                     MMWVTDNTSKNLENAKQDAFKQCEKNGGRNCQMILSFSNMCLAFAQGYKDGQGFEDFG
                     YGIVSSLAEENALENCNQKGGSSCLLSAPAVCAIPCDILSNNKCMYDQPQIIIPGKNN
                     NQPINPPANGNGEHWLSN"
BASE COUNT         1207 a          628 c          826 g         1246 t
ORIGIN      
        1 cgcattaaaa tatcagtatc atttgtcata atgctcggat taatatgaaa ttttcgagca
       61 aaatgaatta aatttcgata actttgtagt aaatgatcaa tcagcatatt ctcatgattc
      121 atcacttgct taattttcca atgttgacgg ttttgaagta attgaatttg tttttctgac
      181 caagaccatt ggttaaccag ctcgtctaaa tcacgacttc tccagttgtt atttatttct
      241 ccctcaaccg cttttgtata aaaacagact cgcaagcggt ctaatttcac ccactctttg
      301 cgtgatctta agtgttctgt taccaattca agcatggcta agtagggatc aaagtgatat
      361 tccaaagcat tgtcacttag tagttttttc ttaaattctt tggagatgag ctttgtttta
      421 ggataagtgt gagcgtagca ttcgagcaat aaaattttaa ttgccgattt ataagggtta
      481 ttaattcctt tgtagagctg ccataagctt gcaccaaaaa attcactggt tgaaaggctg
      541 gaaaaatcgc cgaaatcaat ccattccgtt aagtcaattt cagggttatt gtgaacattt
      601 tggtattcat ttttatgtaa atgcaaccag agtaaacgct taccggcaag taaaatagct
      661 gaacgataaa attcatctaa tagaaaaaaa tgctgtgctg aaccactgtg ttcatcggtt
      721 acgctactgt tataagtttg gctcttaaat tgttctgggt tcattaaata aatattgatg
      781 tcgatttgga atgtctttgc ccattcttgc aatttattaa ttttgagctc cattaactta
      841 tattgttcaa ttgtaaaatg gttcggatag catagccata aatctaaatc agaacagctt
      901 gtttgggtaa tagtaccggt gctacccatc gaataaagag catcaaagct gggatggggg
      961 cccaaatgat cggcagagat tgcttcatca aaacggtaag aaaccagaaa ttgtttttga
     1021 taatcagaga gttgaaaatg ccaaatccct tgtggtgcat tttcaacata agccggtaac
     1081 acagggtgat taagatgaat aagcaatgga atgagagaga aaacatgcat aaaatgatga
     1141 ttattagcat tgatagctcg ttctattcgg tatgcattaa gttgatcaat tcgtgcttta
     1201 gtagagttta atttttgggc gaagctcccc tggttcgccc caatggcagc gccttctacg
     1261 gctgttattt tgttggctgt cagggttaat gttttcacgt cagctttcct tactaaagtg
     1321 tgaactgttt agcattttag cggaaaagtg attattatgt aatagggtaa aaaatcggga
     1381 aattaaattt cattccctat tttgcaaaaa atgtctgaaa ttcaaccgct atcttgatag
     1441 aaagagttca gaccagtaat gaaattgtgt aaaataatca agttttagta gcttatttga
     1501 gtgaacaagt gtaagctgaa tcaagtattt ttcagatcaa aatgatgaag gaaacaaaat
     1561 gaaaagagtc gttattacag gattaggtgt aatttccagt atcggtaaca acaaagaaga
     1621 agtgttggaa tcactaaaag caggtaaatc aggcattgaa tttgtgcctg agtttgctga
     1681 agtgggtatg cgtagccaag tggcaggtac gattaaatta aatccggctg agttaattga
     1741 ccgtaaaatc tatcgtttta tgggagatgc tgcggcttac gcttatcttt caatgaaaga
     1801 agcaatcgaa gactcaggct taaccgaaga acaagtttca aacgatcgta ccggtttggt
     1861 gatcggagct ggtacaggct cggcgcacag tcaacttgta gcttgtgatg cagtacgtgg
     1921 cccacgtggt gtgaaagccg ttgggcctta cgcagtaacc aaaacaatgg catcaagtgt
     1981 gtcagcatgc ttggcaacac cttataaaat tcgtggtgta aactattcaa tcagctcagc
     2041 ttgtgcgact tcagcacact gtattggaca cgcgatggag ttaatccaat taggtaaaca
     2101 agatgtggtg tttgcaggtg gtgcagaaga actttcttgg gaatgtgcaa ccgagtttga
     2161 tgcaatgggt gctgtttcaa ctaaatataa tgaaacacca acaaaagcta gccgtgctta
     2221 tgatgctaat cgtgacggtt ttgtgattgc cggtggtggt gcagtggttg tggtggaaga
     2281 gttagaacac gctcttgctc gtggtgccaa aatctatgct gaaattgtgg gctatggggc
     2341 aacctcagac ggttacgata tggtcgcgcc aagtggtgaa ggtgctgagc gttgtatgag
     2401 acaggcgatg gcaactgtaa atggtgaaat cgagtatatt aacgtacacg gcacttctac
     2461 tccagtgggt gatgtaaaag agttaggggc gatccgtaat gtctttggtg ataaaacgcc
     2521 agcaatttca tcaaccaaat caatgaccgg acactcatta ggtgcagcag gtgcgcacga
     2581 ggcaatttat tcattattaa tgttagataa taattttatt gcaccaagca ttaatattga
     2641 aacccttgat gaacaagcac aaggtatgaa tattgtgact gaacgccaag acaaagcatt
     2701 aacaaccgtg atgtcaaaca gcttcggctt tggtggtact aatgcgtgtt tagtgttcca
     2761 acgctattct aagtaacata aaaagagcaa gtgaaagctt gctctttttt cttttgaggt
     2821 aattattata aatttcaaaa taagttcttt gctatttgtt attttgtcat tatttttgac
     2881 cgcttgttct acaatgatta agccactaga tagtgtttat tggcgtggta aagatgtatc
     2941 tgaatttttt gcacgtaaac gtttacctga tacagcggtt caatgcgtaa aagataatga
     3001 attaagctat gtttatggtt attcaactag ctttgtataa tttgcaaact tatgaagttg
     3061 gtcgtaatgc aaccactata tttatgacaa cagataaggt ttatacaggt ggatcgactt
     3121 ggacatatct ttttgctgac gctgataata aaatcacttc tgttcgtgaa atgaatggtt
     3181 ttggtgatgt cgaaaaagag tttaaatgtg gtgaatataa agaagcggta accattgcca
     3241 gtaaagagga cttagcaaag cgtaaaacac agcctcgttt atggtcatca atttatatgt
     3301 cacctgaaca aaatatgatg tgggtgactg ataatacgtc taaaaattta gaaaatgcga
     3361 agcaagatgc gtttaaacag tgtgaaaaga acggtggtcg taattgccaa atgatcttat
     3421 cgtttagtaa tatgtgcctt gcttttgcac aaggttataa agatggtcaa ggcttcgagg
     3481 attttgggta tggtatcgtt agtagtcttg ctgaagaaaa tgcattagag aattgtaatc
     3541 aaaaaggtgg ttcaagttgt cttctttctg cccctgccgt atgtgctatt ccctgtgata
     3601 tattgtcgaa caacaaatgt atgtacgatc agcctcaaat tattattcca ggtaagaata
     3661 ataaccaacc gattaatcca ccagctaatg gcaacggtga gcattggttg agtaattaaa
     3721 tcattttagt aaaaagtgcg gaaacgcact tttttattgc ctgcaagcgg tcatttttag
     3781 tgaaaacttt gctaaaaatc ttggtaaata aaaatttaca atttattttt aattttattt
     3841 aaaaccattt ttatattcta tcttagtttt taattcttat taaatcattt ttataaatgc
     3901 tttctta
//
LOCUS       DANLUQ010000181         3870 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.182, whole genome shotgun sequence.
ACCESSION   DANLUQ010000181 DANLUQ010000000
VERSION     DANLUQ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.182"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            443..2938
                     /locus_tag="PXB27_002338"
     CDS             443..2938
                     /locus_tag="PXB27_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HDL5347080.1"
                     /translation="MSQAVFYLNNFKELFMTVEVGGVVITADLEFENLLRDTQKVDKA
                     MDDIGKSTLKAEKGLKGLETQATKTAIAVNQATNKMKNIRGVAGQLGYQIQDISIMLQ
                     MGSNASTVIAQQGSQILSIFGPMGAVAGAILAISGAIGGALLPSLFDSTDATELLAAA
                     QKNLSEVVTQTESGVSALSAKIIKLAKVSEDAAKAKIAVAMSDAKIAIKAAGDSSQKA
                     FEQFDGFFGSFTNSSQSIRNAIDELKRYESKGKDVGAVIKELGGTYGGAIAEVNALDS
                     VTSEMSKTLGITQSEALGLIKLLAQLEQDKSPDNIKLLAIALSDLSDKNGWANEELNK
                     LTRTINDNSVSALDAETSVRLLESAIKDLQNAIQNSQGALDGNVAKMNQLADAAERKA
                     ATIGKTERETAKYAATLLATTDEAKKNLDSTLSRIDASYDQIEAYQAEQKALQDKKTA
                     DAKAAREAEAAAKKSAQAKKQVVEQLRQMATQYEIAVLRQKGMHLEAVKLEAGMRLGA
                     AATKEQREEAEKLAQSIYGATAASQNFNAIQAQASPVTALDQRHQQQLAQIEEYKILY
                     PQKIMEAEAARAAIEEQYRQQRMEAQWEEWKQASDGARMFGDAVDAVGSSATSTITGL
                     LNGTMSVRDAFASIANTILNSVVQSLVEMGMAQVKQMIMGQTAAKASMAAQMVQAKAL
                     AASFAPAASMVSLATQGANAIPAQAAITSTMSMAQVAAVTGRKLGGPVSARQMYRVGE
                     NNQPEIFKASNGMQYMIPGQSGRVFSNKQSQGQGGGESKTVAVIVNQTNHFSSDEASA
                     NQAAVAKALSNQIKASVRLELQSQMRAGGVLAR"
     gene            2942..3271
                     /locus_tag="PXB27_002339"
     CDS             2942..3271
                     /locus_tag="PXB27_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5347081.1"
                     /translation="MQTFNFKVEQDYTVQNEPSVVILKFADGYEQTAPKGLNHNLKKY
                     QGITVKANKAEAVKIQQFLNAHGGYKKFNWRDRTSDETVTVRCRSWSSTKLGAVIEFT
                     LNFDEVI"
     gene            3271..3741
                     /locus_tag="PXB27_002340"
     CDS             3271..3741
                     /locus_tag="PXB27_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDL5347082.1"
                     /translation="MPKAPPKKMASELIKLEQDALIELWEIDLTHIYSSSNPNQRGEI
                     FRFHNGVSQTQQNIWWQGNEYQAYPIAADGFEISGQGPSNRPTLTVSNLYGVVTVLVE
                     DFGQPVEAKVSSEETVDGNIQMTVELLKKMDQIADQRYRRNQLNDLRTGGTLSK"
     gene            3749..>3870
                     /locus_tag="PXB27_002341"
     CDS             3749..>3870
                     /locus_tag="PXB27_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDL5347083.1"
                     /translation="MPISISNQMKLDLAKLEQNAMLDLYEVDLRKLQDKNGNAG"
BASE COUNT         1336 a          735 c          884 g          915 t
ORIGIN      
        1 tcttttttca gcaattgtaa taggtttatt cctttttcaa gaatcaagat taagagatcc
       61 aaacaaccat tcagtgatat atttagagca gattcaagaa atgtgcagct tagataaaat
      121 aggctcatac cagattgatt ttgtacgaca aagtgggaat gagtacaaag agagtattct
      181 tgttaatgat tttgagttag caataaaaac cgtattatcc acgttaagaa gggcaaaaat
      241 agatagtgtg ccagtaatat ctaacacact tatttattat ccaccgagct ttctacaacg
      301 caagaggtag tcaagaagga aagaaattag gatcgataag gattgcaaaa atttaatgta
      361 acaaaaatat agattaaatt gtatttaacc aatctattgc tagataaatt gtaaaaaaat
      421 cacagaaata accgcttgtg ctatgtcaca agcggttttt tatttaaata attttaagga
      481 attgtttatg acggtcgaag ttggcggagt tgtaattacc gcagatctag aatttgaaaa
      541 tctgctaaga gatactcaaa aagttgataa agcgatggat gacatcggaa aatcaacatt
      601 aaaagcagaa aaagggctga aagggctaga aacgcaggca accaaaacgg cgatagcagt
      661 aaatcaagcc acaaacaaaa tgaaaaatat ccgtggagta gcaggtcaac ttggttatca
      721 aattcaagac atctcaataa tgcttcaaat gggttctaac gcttcaaccg ttattgctca
      781 gcaagggtcg caaatcttaa gtattttcgg tccaatggga gctgttgcag gggcgatctt
      841 agctattagt ggtgctattg gtggggcgtt actccctagt ttgtttgatt caactgatgc
      901 aactgagctt ttggcagcag ctcaaaaaaa tctcagcgaa gtagttactc aaacggaaag
      961 tggtgtttcc gccctttctg cgaaaatcat taaattagca aaagtaagcg aagatgcagc
     1021 aaaagcaaaa attgcagtag ctatgagtga tgcaaaaata gctattaagg cagcagggga
     1081 ttcatcacaa aaggcatttg agcaatttga cggctttttt ggatcgttta caaattcaag
     1141 tcaaagtatt cggaacgcaa ttgacgaatt aaaacgctat gaatcaaaag gtaaagatgt
     1201 tggtgccgta ataaaagaac ttggaggcac atatggtggt gcaattgccg aagttaatgc
     1261 gctggattct gtgacaagcg aaatgtctaa aacgcttggt attactcaat ctgaggctct
     1321 tggtttgata aaactgttgg ctcaattaga gcaagacaaa agccctgata atattaaatt
     1381 actcgcgata gctttatctg acttaagcga taaaaatggg tgggcgaacg aagagctaaa
     1441 taagctaacc agaacgataa acgataattc agtatctgct ttagatgccg aaacatcagt
     1501 aagactctta gagtctgcta taaaagactt gcaaaatgca atccaaaact cccaaggtgc
     1561 attggatggc aatgtagcaa aaatgaatca acttgcagat gcggcagaaa gaaaagctgc
     1621 aacgataggc aagactgagc gtgaaactgc taaatatgca gctacgctat tagctacaac
     1681 cgatgaagct aaaaaaaatc ttgattccac attaagtagg attgacgcat catacgatca
     1741 aatcgaagcc tatcaagccg aacaaaaagc cttacaagat aaaaagaccg cagatgcaaa
     1801 agctgccaga gaagctgaag cggctgcaaa aaaatccgct caagccaaga agcaagttgt
     1861 cgaacagttg cgacaaatgg ctacacagta cgagattgct gttttgaggc aaaagggaat
     1921 gcatctcgaa gccgtaaaat tagaagctgg tatgcgttta ggagctgcgg caacaaaaga
     1981 gcaacgagaa gaagctgaaa aactagcaca aagtatttat ggagcaaccg cagcaagtca
     2041 gaactttaac gctatacaag cccaagcatc tcctgttact gcattagacc aacgacatca
     2101 acaacagcta gctcagatcg aagaatataa aatcttgtat ccacaaaaaa taatggaagc
     2161 agaagctgcg agagctgcaa tcgaagaaca ataccgccag caacgaatgg aagctcaatg
     2221 ggaggagtgg aagcaagcga gcgacggggc gagaatgttt ggtgatgcgg ttgatgctgt
     2281 tggttcaagt gcaacaagca caattacggg gctactaaat ggcacaatgt ccgtgcgtga
     2341 tgctttcgca tcaattgcaa atacgatctt aaatagtgta gtacaaagcc ttgtcgaaat
     2401 gggtatggct caagttaagc aaatgattat ggggcaaact gctgccaagg cttctatggc
     2461 ggcgcaaatg gtgcaagcta aagcgttagc tgcttcattt gcacccgcgg cctctatggt
     2521 ttctcttgcc acacaggggg cgaatgctat tcccgctcaa gctgcgatca cgtcaactat
     2581 gagtatggct caagtcgccg cggtgacagg tcgtaaactt ggcggccctg tatcagctag
     2641 acagatgtat cgtgtcggcg agaacaatca gcccgaaatc ttcaaagcga gcaatgggat
     2701 gcaatatatg atccccggtc aaagcggtcg tgtattcagc aacaagcagt cgcaaggtca
     2761 aggtggaggc gaaagcaaaa cagtagctgt catcgtaaat caaaccaatc actttagcag
     2821 cgatgaagcc agtgcaaatc aagcggctgt tgcaaaagca ttgagcaatc aaatcaaggc
     2881 ttccgtgaga ttggaattgc agtcacaaat gcgagctggt ggagttctag cgaggtaatc
     2941 aatgcaaaca tttaatttca aggttgagca agactacact gttcaaaatg agcccagtgt
     3001 cgtcatacta aaatttgccg atggttacga gcaaaccgct cccaaagggt taaaccacaa
     3061 tctgaaaaaa taccaaggca ttaccgtaaa agcgaacaaa gcagaagcgg tgaagattca
     3121 gcaatttctc aatgctcacg gtgggtataa gaaattcaac tggcgcgata gaacaagcga
     3181 tgagacggta acggttcgtt gcagaagctg gagctccact aaattaggtg cagtcattga
     3241 gtttacactt aatttcgatg aggtgattta atgccaaaag ccccccccaa aaaaatggca
     3301 tcagagttga taaaactcga acaagatgcc ctgattgagc tgtgggagat agatctcact
     3361 cacatctact ctagctcaaa cccaaatcag cgcggagaga tattccgctt tcacaatggc
     3421 gtgagccaaa cacaacagaa catctggtgg caaggcaatg aataccaagc atacccgatt
     3481 gccgccgatg gttttgagat ttcgggtcaa ggaccgtcaa atcgtccaac actcactgtc
     3541 tctaacctgt acggtgttgt cacggtatta gtagaagatt tcgggcaacc cgtagaggcg
     3601 aaagtcagca gtgaagagac tgtagatggc aatatccaaa tgacggtgga actgctgaaa
     3661 aaaatggatc aaatcgccga tcaacgctac cgtagaaatc agttaaatga tttaagaaca
     3721 ggtggaacat tatctaaata ggtgaagtat gccaattagt atttctaacc aaatgaaact
     3781 agatcttgcc aaacttgagc aaaatgcaat gctggacttg tacgaggttg atttgcgtaa
     3841 attgcaagac aaaaacggca atgctggtag
//
LOCUS       DANLUQ010000182         3862 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.183, whole genome shotgun sequence.
ACCESSION   DANLUQ010000182 DANLUQ010000000
VERSION     DANLUQ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.183"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            254..586
                     /locus_tag="PXB27_002342"
     CDS             254..586
                     /locus_tag="PXB27_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF413 domain-containing protein"
                     /protein_id="HDL5347084.1"
                     /translation="MADSFSVTRRFFDDKNYPRGFSRHGDYTIRESQTLEQFGQACLA
                     LESGERKPATQEEKRFVAVMKGEEVAETDIEKAWLKYRTLTSKTKRIYTLSGNAGSDA
                     GDDFSAAE"
     gene            651..1742
                     /gene="trmA"
                     /locus_tag="PXB27_002343"
     CDS             651..1742
                     /gene="trmA"
                     /locus_tag="PXB27_002343"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HDL5347085.1"
                     /translation="MTLPIEQYSDLLAKKTENLTALLKPFNPPAIEVFASKPSHFRMR
                     AEFRVWHEEGELYHIMFNPETKARYRVDSFPIASELINHMMTALLAEIKGNELLTRKL
                     FQIDYLSTLSGEIAVSMLYHKSLNEEWVEQANALKSRLEQQGFKVQIIGRANKQKIAL
                     DCDYVEEILPVHGKNYVYRQVENSFTQPNAEMNIKMLEWARNCTKNSEGDLLELYCGN
                     GNFSIALAENFRKVLATEISKSSVQSAQYNIEKNGIVNLQIIRMSAEEFTQAMNGVRA
                     FYRLKGIDLNAYECNTIFVDPPRAGLDQATLNMVQAYERILYISCNPETLAENLQQLS
                     QTHRIEKAALFDQFPYTHHIESGVWLIRK"
     gene            1834..1923
                     /locus_tag="PXB27_002344"
     tRNA            1834..1923
                     /locus_tag="PXB27_002344"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1868..1870,aa:Ser,seq:tga)
     gene            2079..2450
                     /locus_tag="PXB27_002345"
     CDS             2079..2450
                     /locus_tag="PXB27_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HDL5347086.1"
                     /translation="MKYLYIICGFICIGLGIIGAVLPGLPTTPFLLLALFCFTKGSER
                     IRQWFIQTKLYKKYLQEYDEKRAMTMKQKITILAISFPFTLFAFFALPHIGGKIALVV
                     LVIIQYWYFFCKMKTLEPENA"
     gene            complement(2447..3358)
                     /gene="xerC"
                     /locus_tag="PXB27_002346"
     CDS             complement(2447..3358)
                     /gene="xerC"
                     /locus_tag="PXB27_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDL5347087.1"
                     /translation="MTTSNPLFQQTRPYWDFLRIEKQVSPHTLSNYQRQLMAVSELFS
                     QAGIENWEEVESSSVRWMLSQSHKQGLSAKSIGLRLVALRQWFVYLIKIGRMSANPTL
                     GVKSPKVGKHLPKNIDAEQVGQLLAIESDEPSDIRDIAMMELMYSSGLRLSELQGLDL
                     GDMDLSAREVRVQGKGSKERIVPIGSQALKALNRWLEMRLQFKPQDNAVFLNKRGGRL
                     SHRSIQLAMKKWGERQGLESHLHPHKLRHSFATHMLEASGDLRAVQELLGHSSLSTTQ
                     IYTHLDFQHLAKIYDSAHPRARRKQED"
     gene            complement(3425..3715)
                     /locus_tag="PXB27_002347"
     CDS             complement(3425..3715)
                     /locus_tag="PXB27_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5377 domain-containing protein"
                     /protein_id="HDL5347088.1"
                     /translation="MSVKTEVLFSNHWNVRVSDPGFEGSPSHYFETVYLTLEAFINGE
                     NVHYEFTRKVEQEVKTHRSFTDVNELFEFLGEYLSPVALGNVGVRIGQLGLA"
BASE COUNT         1157 a          843 c          739 g         1123 t
ORIGIN      
        1 cacagtatat ctccaaatat taaagttaat caaaactcaa tacatttagg taatatacct
       61 taacaaatat aaaaaatata cctcagactg cattgtctat tttaaaagga tcaatatttc
      121 tccttaaaga gttttttgca aaaaatttgc caaatttgac cgctttttta cattaaggca
      181 ttgatttatt cgctgttttt gtttaatgta tgccgatttt attttcccta atttcaaaca
      241 acgaggcatt attatggcag acagtttcag cgttacgcgc cgcttttttg acgataaaaa
      301 ttatccacgt ggtttctctc gccacggcga ttacaccatt cgtgaatcac aaactttaga
      361 acaattcggt caagcttgct tggctttaga aagtggtgaa cgtaagccgg caactcaaga
      421 agaaaaacgt ttcgttgcgg taatgaaagg cgaagaggta gcagaaactg acattgaaaa
      481 agcgtggtta aaataccgca cattaactag taaaacaaaa cgcatctaca ccctttcagg
      541 caatgcaggt agcgatgccg gcgatgactt cagtgctgct gaataatttc taatttaaaa
      601 ccacgaagta attcgtggtt tgttttttca tttgctattt gtggtaaaaa atgactcttc
      661 ctatcgaaca atattccgat cttcttgcaa aaaaaaccga aaatttgacc gctttactca
      721 agccgtttaa cccgcctgcg attgaggtat ttgcctcaaa gccaagccat ttccgtatgc
      781 gggcagaatt tcgagtgtgg cacgaggaag gcgagctgta ccacattatg ttcaaccctg
      841 aaaccaaagc tcgttatcgt gtggatagct tccctattgc cagtgagctg attaaccata
      901 tgatgaccgc attattggcg gaaattaaag gcaatgaact actgactcgc aagctgttcc
      961 agatcgatta tttaagcacc ttaagcggtg aaattgcagt ctcaatgctt taccacaaaa
     1021 gcctgaatga agaatgggtt gaacaggcaa acgccttaaa aagccgttta gagcaacaag
     1081 gtttcaaagt gcaaattatc gggcgtgcca ataaacaaaa aatcgcactg gattgcgatt
     1141 atgtggaaga aattctaccg gtacacggca aaaattatgt gtatcgccaa gtggaaaaca
     1201 gctttaccca gccgaatgcg gaaatgaata ttaaaatgct ggaatgggcg agaaattgca
     1261 caaaaaatag tgaaggcgat ttgcttgagc tttactgcgg caacggcaat ttttcgattg
     1321 ccttggcgga aaacttccgc aaagtgctgg caaccgagat ctcaaaatct tcagtgcaat
     1381 cggctcaata caatattgaa aaaaatggca ttgttaacct acaaattatc cgaatgtcgg
     1441 cagaagaatt tacccaagcg atgaacggtg tgcgagcctt ttatcgctta aaagggattg
     1501 atttaaacgc ctacgaatgc aataccattt ttgttgatcc acctcgtgca gggttagatc
     1561 aagctacatt aaatatggtg caagcgtatg agcggattct ctatatctct tgcaatccgg
     1621 aaacccttgc cgagaattta caacaattaa gccaaaccca tcggattgaa aaagccgctc
     1681 tttttgacca attcccttac acccaccata ttgaaagtgg cgtttggtta attagaaaat
     1741 aaattgtgct gaaaaaaaga acgaataagc agcttttaag atttattact tgacgatgtt
     1801 cgcaataatc tctattattg caacgttttc aacggaggaa tggcagagcg gttgaatgca
     1861 ccggtcttga aaaccggcga gggttaacgc cctccgtgag ttcgaatctc acttcctccg
     1921 ccagtcttta aacctgttga gcaatcagca ggttttttta tttcatttgc aagaaaatca
     1981 gataaaacca ccgcttgaat ccatcgctat aaactggttg aaatcagcct aaagttgctt
     2041 taggataatt gcgattcatt atcaataact taattattat gaaatacctt tatattatct
     2101 gtggttttat ttgtattggt ttaggcatca tcggggcagt attaccgggc ttgccgacca
     2161 caccgttctt acttttggct ctgttctgct ttactaaagg ctcggagaga atccgccagt
     2221 ggtttattca aaccaaatta tacaaaaaat atctgcaaga atatgatgag aaacgggcaa
     2281 tgacgatgaa gcaaaaaatc acgattttag cgatttcgtt tccgtttaca ctatttgctt
     2341 tctttgcact gccccatatc ggtgggaaaa ttgcgttagt agtgttagtg ataattcaat
     2401 attggtattt cttctgcaaa atgaaaaccc ttgaaccgga gaatgcctaa tcttcctgct
     2461 tccgccttgc tcttgggtgg gcagagtcat aaattttagc taagtgttgg aaatccagat
     2521 gcgtataaat ttgagtggtg gacaggctac tatgacccaa taattcctga accgctcgca
     2581 aatcaccgct tgcctctaac atatgagtcg caaaagagtg acgtaattta tgtggatgca
     2641 gatggctttc cagcccttgt cgctcacccc atttcttcat tgccaactga atggaacgat
     2701 gagataaccg cccacctcgc ttattcagaa acaccgcatt atcttgcggc ttaaattgca
     2761 aacgcatttc taaccagcga tttaacgctt ttaacgcttg cgagccaatc ggcacaattc
     2821 gctctttgct acccttacct tgcacccgta cttctcgtgc cgataaatcc atatcgccta
     2881 aatccagccc ttgtaattcc gataaacgca agccggaact atacatcagc tccatcatcg
     2941 caatatctcg aatatcgctc ggctcatcac tctcaatcgc cagcaactgc ccgacctgct
     3001 ccgcatcaat atttttcggc aaatgcttac ctactttcgg cgatttcacc cctaaagtcg
     3061 ggtttgccga cattcgcccg attttaatca aatagacaaa ccattgccgt aacgccacca
     3121 agcgtaagcc gatacttttc gcactcaacc cttgcttatg gctttggctt aacatccaac
     3181 gcaccgagct actttcgacc tcttcccaat tttcaatgcc tgcttgcgaa aaaagctcgc
     3241 tcaccgccat taattggcgt tgataattgc ttaaggtatg cgggctaact tgcttttcaa
     3301 tgcgtaagaa atcccaatag gggcgggttt gttggaaaag agggttggaa gtggtcataa
     3361 acgtaataac aagcggttaa tttttaggaa aatgttgcaa gggcaaaaga tttgcccttt
     3421 gagattaggc tagccccaac tgaccgatac gcacgccaac attaccaagt gcaacagggc
     3481 ttaaatattc gccgagaaat tcaaacaatt cattcacatc agtaaaactg cgatgagttt
     3541 tcacttcttg ctccacttta cgagtaaatt cataatgtac attttcgccg ttaataaagg
     3601 cttccagcgt taaatatacg gtttcaaaat agtggcttgg cgagccttca aagcccggat
     3661 cactcactcg aacattccaa tgattactaa ataaaacttc ggtttttact gacattgtgt
     3721 tttccttctt cctataagta cgtacattat tttaatcaag atagattgaa tttgccactt
     3781 ttcctagtca gaatcgaggc aaatcttatt tggtttgtcc tcttatatct ttccctcaaa
     3841 atatgatctt ggtcaaaaaa ta
//
LOCUS       DANLUQ010000183         3740 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.184, whole genome shotgun sequence.
ACCESSION   DANLUQ010000183 DANLUQ010000000
VERSION     DANLUQ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.184"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            70..1254
                     /locus_tag="PXB27_002348"
     CDS             70..1254
                     /locus_tag="PXB27_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HDL5347089.1"
                     /translation="MSRPLNFVMISPHFPTNFETFAIRLREAGFNTLGIADAPYESLS
                     ENLRHSLTEYYRVDNMEDYEQVYRAVAYFAHKYGRIDRIESHNEYWLELDAKLRTDFN
                     VFGYKSDDMLSIKTKAQMKEVFRKTGLKVAQGRVFKDDEDARKLAKQLKFPVIIKPNS
                     GVGASDTYKIKTAEELEAFFGYKTPTVEYIMEEFIDGDIITFDGLTDKEGNIVFYSSL
                     EYSEAVLDTVSKDGDMFYYVPCEISPKLVEMGKQCVKAFNVKERFFHFEFFRVKGSKK
                     EELLPLEINCRPPGGLTIDMWNYANDFDVFREFANIVKENKFYADIQHPWNVVYISRK
                     ANQNYVNSIEAVCEKFAGNIISVQSVPGVFAKVMGEQGILARTETMEQMREIAQFAQA
                     KQ"
     gene            1265..2017
                     /locus_tag="PXB27_002349"
     CDS             1265..2017
                     /locus_tag="PXB27_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDL5347090.1"
                     /translation="MHFEKRSHWSGELGREMSFNVYGHAGKPVIVFPSSGGNENEYGN
                     FGMIEACRSFIERGLIKFYTPDSYDAESWLSKHKSGHDMALAHNAYDRYIIHELVPLI
                     RHESHWNGTMIATGCSMGAFHTVNFALRHPDLFDIAIALSGVYDARFFSGEFYGDKAV
                     YFNSPIDYVWGQNDDWFLDRYRQNHYVIAVGQGAWEEQHIEDTRRLQEAFNAKGIDAW
                     FDYWGFDIDHDWPTWRKQMPYFLGKLEEQGII"
     gene            2038..2490
                     /locus_tag="PXB27_002350"
     CDS             2038..2490
                     /locus_tag="PXB27_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HDL5347091.1"
                     /translation="MNLKAVAVSALAMSSVAMADIADVKPVVFESKFDFQTTVTTLTN
                     TLKEKGMTIFAEIDHQAAAKQAGLEMQPATVLVYGTPKAGTPLMVKDPMLALQLPLKV
                     LITEPNKGKVEVMLNRADQVVAHANTDYADVENNLAKAEKLIKATVAK"
     gene            2594..3688
                     /locus_tag="PXB27_002351"
     CDS             2594..3688
                     /locus_tag="PXB27_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5347092.1"
                     /translation="MKLNQAIADRIVKRTMQIIPNSVNVMDSEGVIIASGDVGRIGQR
                     HTGAVIALRNDQAVEIDENLAKQWCDEVKAGINLPLHYLGSTIGVVGISGQPQQVRQY
                     AQLVKMAAELIMQQAFELEQERWQRRYREEFVRALLKGSLSTAEIQQQAVFFGQDFAK
                     PLSVMVIKIHGSTAEKLQRLIDFFEHYFPSLLTAVIGLDKIALLNHLSNEKSLIRNAL
                     NKLNDPDISFKTVVGLAVENLSQTHISYQTACHTLNYAEQIQSRKQIIHFEDFKLPAL
                     LHSFSHSWEAKTLLSPFQALLVSDKKQVLLKSLRYYFFANCDLDHAAKKLFIHPNTLR
                     YRLEKIEQITGLSFNNIEQKFVLYLGAVLS"
BASE COUNT         1131 a          735 c          849 g         1025 t
ORIGIN      
        1 attaataaat ttagcctaaa atctattcta atcattttaa taatatgcaa actttataaa
       61 ggaagaacta tgtctagacc acttaatttt gtgatgattt ccccgcactt cccaacgaac
      121 tttgaaacct ttgccattcg tttgcgtgaa gccggtttta atacattggg aattgcagat
      181 gccccttatg aatcattgag cgaaaatctg cgtcattccc tgactgaata ttatcgtgta
      241 gataatatgg aagattacga acaagtttat cgtgcggtcg cgtactttgc ccataaatac
      301 gggcgaattg accgcattga atcgcacaat gaatattggc tggagttaga tgcgaaattg
      361 cgtaccgatt tcaatgtgtt tggttataag agcgatgata tgctctcaat taaaaccaaa
      421 gcccaaatga aagaggtatt ccgcaaaacc ggcttgaaag tggcacaagg gcgagtattt
      481 aaagatgatg aagatgcccg caaattagca aaacagctta aattcccggt gattatcaag
      541 ccaaattcag gggttggggc aagcgatact tacaaaatca aaactgcgga agagttagaa
      601 gctttcttcg gttataaaac gccaacggtt gaatatatta tggaagagtt tattgacggc
      661 gatattatta cctttgacgg tttaaccgat aaagagggca atatcgtttt ctactccagc
      721 cttgaatatt cagaagcggt attagatacc gtttcaaaag atggcgatat gttctactat
      781 gtcccatgtg aaatttcgcc aaaattagtg gaaatgggca aacaatgtgt gaaagccttt
      841 aacgtaaaag agcgtttttt ccattttgag ttcttccgtg ttaaagggag caaaaaagaa
      901 gagttgttac cactcgaaat caactgccgt ccgccgggtg gcttaactat tgatatgtgg
      961 aactacgcca acgatttcga tgtgttccgt gaatttgcca atattgtgaa agaaaacaaa
     1021 ttctatgccg atattcagca cccttggaac gtggtttata tttcccgcaa ggcaaaccaa
     1081 aattatgtga attcgattga agctgtttgt gagaaatttg ccggcaatat tatcagcgtg
     1141 caaagcgtac cgggcgtatt tgccaaagtg atgggcgaac aaggcatttt agcccgtacc
     1201 gaaacaatgg agcaaatgcg tgaaattgca caatttgcac aagctaaaca gtaagaggtg
     1261 aagtatgcat tttgaaaaaa gaagccactg gagtggagaa ttaggtcgtg aaatgtcgtt
     1321 caacgtttac ggacacgcag gtaaaccggt tattgttttc ccttcctccg gcggcaatga
     1381 aaacgagtat ggtaattttg gaatgattga ggcttgccgt tcctttattg aacgtggctt
     1441 aatcaaattt tacacgcctg attcttacga tgcggaatct tggttatcta aacataaatc
     1501 cggacacgat atggcattag ctcataatgc ttacgaccgt tatattattc acgagctagt
     1561 gccgttaatc cgccatgaat cgcactggaa cggcacgatg attgccaccg gttgtagtat
     1621 gggggctttc catacggtaa actttgcctt acgtcatccg gatctgtttg atattgccat
     1681 cgccttaagc ggtgtttatg acgctcgctt tttcagtggt gagttttatg gcgataaagc
     1741 ggtttatttt aactcgccga ttgattatgt atggggtcaa aatgacgatt ggttcttaga
     1801 ccgctaccgc caaaatcact atgtgattgc agtcggacaa ggtgcgtggg aagaacagca
     1861 tattgaagac acccgccgat tacaagaggc ttttaacgca aaagggatcg atgcttggtt
     1921 tgactactgg ggctttgata ttgaccatga ctggccgact tggcgtaaac agatgcctta
     1981 tttcttaggc aagttggaag aacaagggat tatttaatct atttgaggag aaaagaaatg
     2041 aacttaaaag cagtcgcagt ttctgcatta gcaatgagca gcgtggcaat ggcagatatt
     2101 gccgatgtca aaccggtggt atttgaaagt aaatttgatt tccaaactac agtcacaact
     2161 ttaaccaaca ccttaaaaga aaaaggaatg acgatttttg ccgaaatcga tcaccaagcg
     2221 gcggcaaaac aagccggctt agaaatgcaa ccggcaaccg tattggttta cggcacaccg
     2281 aaagccggta cgccattaat ggtgaaagat ccgatgttag cgttgcaatt accgttaaaa
     2341 gtgttaatta ccgaaccgaa caagggcaag gtcgaagtaa tgttaaatcg tgccgatcaa
     2401 gtggtggctc acgctaatac cgattatgct gatgtagaaa acaaccttgc caaagcggaa
     2461 aaattaatta aagcgactgt ggcaaaataa cttcacgcta tgatctattc tcaggaagtg
     2521 gaagcgtaga taatttgcaa atccctcgaa agagggattt ttcatagtat tgggaatgag
     2581 taggaatcat ataatgaaac tcaaccaagc gattgccgac cgcatcgtca agcgcacgat
     2641 gcagattatt cccaactccg tcaatgtgat ggatagtgaa ggggtgatta ttgcttccgg
     2701 cgatgtcggt cgtatcgggc aacgacacac aggagcggtg attgcgttac gcaacgatca
     2761 agcggtcgaa attgatgaaa atcttgcaaa acagtggtgc gatgaagtga aagcgggcat
     2821 taacttaccg cttcactatt taggctcaac cattggtgta gtggggattt cagggcagcc
     2881 acaacaagtt cggcaatatg ctcaattggt gaaaatggcg gcggagctga ttatgcagca
     2941 agcgtttgag ttggaacagg aacgttggca acgccgctat cgggaagagt ttgtgcgggc
     3001 cttgctcaaa ggtagcctaa gcacagcaga aatccagcaa caagcggtct ttttcgggca
     3061 agattttgca aaaccgctgt cggtgatggt gattaaaatt cacggctcta ctgcggaaaa
     3121 actccaacgc ctgattgatt ttttcgaaca ttatttcccg agcctactca ccgccgtgat
     3181 tgggttagat aaaattgcct tgctgaatca tttatccaac gaaaaatcgt taatccgcaa
     3241 tgcactaaac aaacttaacg atccggatat ttccttcaaa acggtggttg ggctggcggt
     3301 cgaaaattta tcgcaaaccc atatttccta ccaaaccgcc tgccataccc tcaattatgc
     3361 cgagcagatc caaagccgta agcagattat tcattttgaa gattttaaac tgccggccct
     3421 cttgcacagt ttcagccact cttgggaagc gaaaaccttg ctctcgccgt tccaagcttt
     3481 actggtttcc gataaaaaac aggtgttact gaaaagtctg cgttactatt ttttcgcaaa
     3541 ttgtgatctg gatcacgctg ccaaaaaatt gtttattcac ccaaatacgc tacgttaccg
     3601 tttagagaag attgagcaga taactggttt atctttcaat aacatagagc aaaaatttgt
     3661 gctgtatctt ggagcggttc tgtcgtaaaa attgtaaaaa tatacaaaca agcggtcgtt
     3721 tttaacaaat tttttgcaaa
//
LOCUS       DANLUQ010000184         3715 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.185, whole genome shotgun sequence.
ACCESSION   DANLUQ010000184 DANLUQ010000000
VERSION     DANLUQ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.185"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(76..1578)
                     /gene="ppx"
                     /locus_tag="PXB27_002352"
     CDS             complement(76..1578)
                     /gene="ppx"
                     /locus_tag="PXB27_002352"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HDL5347093.1"
                     /translation="MSSNIAPREFAAIDLGSNSFHMIVARIVNGSIQVLSRLKRRVRL
                     ADGLDENLVLSQDAIDRGIACLALFAKRLEGFSAENVKVVGTYTLRRAINNQAFLEQA
                     KAVFPYPISIISGQQEARLIYSGVSHTQPETGRKLVVDIGGGSTEMTIGDDFTPIRAE
                     SRHMGCVSFAKRFFPQGELTIERFDSAYQLAMEKIEDLMWEYKELGWDYALGSSGTIK
                     TVHQVLIANGYRDGLITESRLQKIIDICLEFKSLNEIKLRGLLDERADVLVPGLAILL
                     ALFHTFRIDVLRYSNGALREGVMYGLEKSFQVGDIRQRTAEALAEQFALDKQQAKRVE
                     HTALILFDQIKTWKNRRQVQDLRSMLKWASLLHEIGIVVNHNNVHRHSAYLIANRDLP
                     GFDFEQQTLLSVLMRYHLKGFKRSDIRSINRYQCRDVLTLVRVFRLAVLLNRSRQATV
                     VPKQLNLVIENNAWQLTFEQGFLANNPLISIDLEEEQKILASTNLQFAVS"
     gene            1774..2103
                     /locus_tag="PXB27_002353"
     CDS             1774..2103
                     /locus_tag="PXB27_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HDL5347094.1"
                     /translation="MSEVNKSFHDMLEYVHLYRLKNKLHRETADNDRKIRDNQKRVLL
                     LDNLTQYITDEMSIEEVRAIISNMRDDYENRVDDYMIRNAELSKQRREIRQKMASHKQ
                     APTNKKA"
     gene            complement(2167..3597)
                     /locus_tag="PXB27_002354"
     CDS             complement(2167..3597)
                     /locus_tag="PXB27_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIBC"
                     /protein_id="HDL5347095.1"
                     /translation="MSTLTYLQKIGRALMVPVAALPAAALLMGIGYWIDPDGWGTNSQ
                     LAALLIKSGAAIIDNMSILFAVGVAFGMSKEKDGAAALSGLVGYLVITTLLSPGTVAQ
                     LKGIAVDQVPAAFGKINNQFIGILVGVISAEIYNRFYQVELPKALSFFSGKRLVPIVV
                     SFILLYVWPVIFGALVSFGESIKDMGALGAGIYAFFNRLLIPVGLHHALNSVFWFDVA
                     GINDIPNFLGGAKSLAEGKAVIGVTGMYQAGFFPMMMFGLPAAALAIYHSAKPAKKMQ
                     VASIMFAAALASFFTGITEPLEFAFMFVAPVLYVVHALLTGLSVYIAASMEWIAGFGF
                     SAGLVDLVLSTRNPLAVQWYMLLAQGIVFAVIYYVLFRVVIQAFNLKTPGREEDSEVQ
                     TVNTVSRSEQAAKFVEALGGKENITSIDACITRLRLTLNDTAKADETKLKALGSKGNI
                     KLGENGYQVILGPEAEFVADAMKKLN"
BASE COUNT         1199 a          849 c          648 g         1019 t
ORIGIN      
        1 ctaaatttga ccgcttgttc gcaaaaggga atgcaataaa cgaaaaagga agccttcgct
       61 tccttcattt aaatgttaag agaccgcaaa ctgaagattt gtactcgcca atattttttg
      121 ctcttcttct aaatctattg aaatcagtgg attattcgct aagaagccct gttcaaaggt
      181 aagttgccaa gcattatttt caatcaccaa attcaactgt ttcggcacta cagtcgcttg
      241 gcgagaacgg tttaatagca ccgctagccg gaaaacccgt accaacgtta atacatctct
      301 acattggtaa cggttgatac tacgaatatc cgagcgtttg aatcctttaa ggtggtatcg
      361 cattaatacc gatagaaggg tttgctgttc aaaatcaaat cccggtaaat ctcggtttgc
      421 aatcaaataa gcagaatgac gatgcacatt attgtggttt acaacaatac caatctcgtg
      481 taataaactt gcccatttca gcattgaacg cagatcttgc acttggcggc ggttcttcca
      541 agttttgatt tggtcaaata aaatcaacgc cgtatgctcc accctttttg cttgctgttt
      601 atctaaagca aattgctcag ctaacgcttc tgcagtccgt tggcgaatat ccccaacttg
      661 gaaacttttc tccaaaccat acattacccc ttctcttaaa gccccgttgg aataacgtaa
      721 tacatcaatt ctaaaagtgt gaaaaagtgc taaaagaata gctaagcccg gaactagcac
      781 atctgctcgt tcatcaagta agcctcgtag cttaatctca tttaacgatt taaactctag
      841 gcaaatatca ataattttct gtaggcggct ttctgtaatt aagccatcac gatagccatt
      901 ggcaatcagc acttgatgga cggttttaat cgtaccggaa gaacctaaag catagtccca
      961 acctaactct ttgtactccc acattaaatc ttcgattttt tccattgcaa gctgataggc
     1021 actatcaaaa cgctcaatcg ttaattcccc ctgcgggaaa aagcgttttg caaaactcac
     1081 acaacccata tgacgacttt ccgctcgaat cggggtaaag tcatcaccaa tagtcatttc
     1141 cgttgaacca ccgccaatat ctaccactaa ttttctgcca gtttcgggtt gggtatggct
     1201 aacgcccgaa taaatcagcc tcgcctcttg ttgtccggaa ataatggaaa tcgggtaagg
     1261 aaatactgct ttcgcttgct ccagaaaagc ttggttattg attgctctac gtaaggtata
     1321 agtgccaacc accttcacat tctctgcaga gaacccttct aaacgttttg caaataaagc
     1381 taaacaagca atgcctctat caatcgcatc ttggcttaat actaagtttt catcaagccc
     1441 atctgccagc cgtacacgcc gtttcaaacg ggaaagcact tgaatggaac catttacgat
     1501 acgggctaca atcatatgaa agctgttcga gcctaaatca atagctgcaa actctctagg
     1561 agcaatattt gatgacataa tgaaaccctg taccaataac tgaaataagt aagatattac
     1621 catttttgag ccctagatat cactacaaat ttagcgttat tcgcgagaat taatttaaat
     1681 cataaaatgt gataaatttt tttgacgaat tgacggaaaa agaataggat ttacgagctt
     1741 acttaattca acactacatc aagggagatt gatatgtctg aagtgaataa atcattccac
     1801 gatatgctgg aatatgtgca tctttatcgt ctaaaaaaca aactgcaccg tgaaacggcg
     1861 gataatgatc gcaaaattcg agataatcag aagcgtgtac ttttattaga taacctaacg
     1921 caatatatta ccgacgaaat gtctatcgag gaagttcgag cgattatttc caatatgcgt
     1981 gatgactatg aaaatcgagt agatgattat atgatccgta atgcggaact ttccaaacaa
     2041 cgccgtgaaa tccgccaaaa aatggcctct cacaaacaag caccaaccaa taaaaaagca
     2101 taaaaagcca agcggtctga tttgcaaaaa aatatgcaaa tcagaccgct tgtgattaag
     2161 cagagattaa ttcagctttt tcattgcatc tgccacaaat tctgcttccg gtcccaaaat
     2221 cacttggtag ccattctcgc ctaatttgat attacctttt gaccctaacg ctttcaattt
     2281 ggtttcgtct gctttcgccg tatcatttaa ggttaggcgt aagcgggtaa tacaagcatc
     2341 aatgctggta atattctctt taccacctaa tgcttcaaca aatttggcag cttgttcgct
     2401 acgagaaaca gtattcactg tttgtacttc gctatcttcc tcacgccccg gcgtttttaa
     2461 attaaaggct tggatcacta ctcggaacaa tacatagtaa attactgcga atacgatacc
     2521 ttgggccaat aacatatacc actgcactgc cagcggattt cgtgtcgata acactaaatc
     2581 gaccaaccct gcactaaaac cgaaaccggc aatccattcc attgaggctg caatataaac
     2641 agataatccg gttaataatg cgtgaaccac ataaagcaca ggtgcaacaa acataaaggc
     2701 aaattcaagc ggttccgtaa tgccggtgaa gaacgaagct aaagcagcag caaacataat
     2761 agaggctact tgcattttct tagccggttt cgcactgtgg taaatagcta atgctgccgc
     2821 aggtaagcca aacatcatca tcgggaagaa accagcttga tacatacctg ttactcctat
     2881 cactgctttg ccctcagcca atgattttgc cccacctaag aagtttggaa tatcattaat
     2941 gcctgcaaca tcaaaccaga aaaccgagtt taatgcgtgg tgtaatccaa ccggaattaa
     3001 taaacgatta aagaaagcat aaatacctgc ccctaacgcc cccatatctt tgatagattc
     3061 cccaaaagag actaaagcac caaaaataac cggccagaca taaagcaaga taaatgacac
     3121 cacaatcggc accaatcgtt taccgctaaa gaaagacaat gctttcggta actctacttg
     3181 gtagaaacgg ttataaattt cggctgaaat aacccctact aagatcccga taaattggtt
     3241 gttgatttta ccgaatgctg caggcacttg atcaactgcg atccctttta gctgagcaac
     3301 ggtccccgga gacaacaaag tggtaattac taaatacccg actaaaccgg ataatgctgc
     3361 tgccccatct ttttcttttg acataccaaa tgccacaccc acggcaaata gaatagacat
     3421 attatcaata attgccgccc cagatttaat cagcaatgcc gctaattggc tatttgttcc
     3481 ccagccgtcc ggatcaatcc aatagcctat acccatcaat aatgctgctg ctggtaatgc
     3541 agcaaccggc accattaatg cacgcccaat tttttgtaaa taggttaatg tactcataaa
     3601 agcctccaaa aatacctcat aatgagtaac ttaagcatag aaaaaaaccg attaaagaac
     3661 aatcaaataa atttggtttt gtgatccatt tcaaaatatt ttttaggagt tttta
//
LOCUS       DANLUQ010000185         3667 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.186, whole genome shotgun sequence.
ACCESSION   DANLUQ010000185 DANLUQ010000000
VERSION     DANLUQ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.186"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..2050
                     /locus_tag="PXB27_002355"
     CDS             <1..2050
                     /locus_tag="PXB27_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HDL5347096.1"
                     /translation="LITLAIVAPQVLAETTSQAELNAIEVKEKLAATTGYIPVYTTVG
                     TKTLTSLARTPFSINVVSDDLMHDQAVRSVTDSVAYTSGMMGGYRGNNSLIEISIRGI
                     GNKSDGSTIPTYLNGSRYQTAFEIDPFFLESINVIKGPNSILYGQANPGGVMDIITKK
                     AGSNPHRHLQFVVGNHQRYQIGLDIEENLSNTLSARLIGQLERTNWNTQFVKEKGGAF
                     APSILWKPTTNTELNVYAYYAKKPKAGDRNFLPKEGTINSVNGKKLPYDMFLSDLNYH
                     GLKAEQMQVGYSFSHKFTPNLTFRQNASYHNGEENFKNLVYWGDVGSVLERRARHWDV
                     KTQEINLDNQLEWVFSTANINHTLLAGIDYKRVRKDLDNYMGIAPDFDWQNPVYGVTV
                     TEPSLFSSEVKHLKQLGVYLQDQIEIGNFDFLLGGRYDRAKNSNQDRLNSKTENNQEG
                     KFTWRTGVVYNFENGIAPYISYSTSFVPSAEKDAQGNFLKPTTAGQWEVGMKYQPTQD
                     TLLTLAGFKITQKDLANYQWQTRSYEQIGEVQTKGIEVSLNQQLSDKFSIAASYAYLK
                     KEITADKDGTTIGKTQWGVPRHQASLWAKYKFFDKLTAGVGVRYMGTTFGDNQNTFTV
                     PAYTLYDMMLAYDFMPNLNLQLNAQNLTNKKYVASCANNFSCFYGKDRNLSATLNYHF
                     "
     gene            2094..3530
                     /locus_tag="PXB27_002356"
     CDS             2094..3530
                     /locus_tag="PXB27_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3327 domain-containing protein"
                     /protein_id="HDL5347097.1"
                     /translation="MKKTTACILTFLVSSVLYANNITIELKQNDFVQGKLIQKAQQPL
                     SLSIIFPDQSSRLLAQNVFGEQDFMFKSELIGTATFSILANGATVSDQDFTLTIERHI
                     PISAQIAPPKVIENTQLEELSQKIAKNPEKQTALINDFWDKAKQAGTPLIEPLDEQQS
                     RVTFLWRGATKNARIWGGVSTEHDFMQRFGETDIWYKSYIVPNDTLVEYKLAPDVPTL
                     PVDENTQRRALLATAQADPLNKTAYFEKIGQNIHNTDKFNYASLLKLPNAPTQSFLTE
                     APNIAKGKLQQLIFESATLGNKHRLFVYLPDGFSAEKNYAVLYLFDGVEYTERVPTKT
                     ILDNMIAQKVILPTVAVFIENPSSESRRVELPANPIFTQVLAKELVPFVEKQLNIKTK
                     ERIIGGSSYGGLSSAYTVLNYPNVFSKALILSGSFWWQPKGTPSEQSHYIAQQFIQKE
                     KLPLCFFISAGFYESGKSAICKRSNYFA"
BASE COUNT         1207 a          742 c          691 g         1027 t
ORIGIN      
        1 tctaatcact ttagcgattg tagctcctca agtattagca gaaacaacct ctcaagcaga
       61 actaaatgct attgaggtca aagagaaatt ggcagctacc accggctata ttcctgttta
      121 tacgactgta ggaacgaaaa cattaacctc tttggctcgt acacctttta gtatcaatgt
      181 tgtctctgat gatttaatgc atgaccaagc tgttcgttct gttaccgatt cagtggctta
      241 tacctccggt atgatgggtg gctatcgtgg taacaattcg cttatcgaaa tctccattcg
      301 aggcattggc aataaaagtg atggcagtac aattccgacc tatttaaacg gttctcgcta
      361 ccaaactgca tttgaaatcg atccgttctt cttagaaagt atcaatgtga ttaaaggtcc
      421 aaactccatt ttatacgggc aagctaatcc cggtggcgta atggatatta tcaccaaaaa
      481 agccggcagt aacccacatc gccatttgca atttgtggtg ggaaaccatc aacgatacca
      541 aatcgggctg gatattgaag aaaatctctc caatacctta tccgctcgtt taatcggtca
      601 attagaacga acaaactgga atacccaatt tgtaaaagaa aaaggcggtg cttttgctcc
      661 aagcatttta tggaaaccga ctacaaatac agaacttaat gtttacgctt actatgcaaa
      721 aaaaccgaaa gccggtgatc gtaacttttt accgaaagaa ggaacgatta atagcgtaaa
      781 cgggaagaaa ctaccctatg atatgttcct cagcgatctg aactatcacg gtttaaaagc
      841 cgaacaaatg caagtaggtt acagtttctc acataaattt acgccaaacc taacgttccg
      901 ccaaaatgca agttaccata atggcgaaga gaatttcaaa aatctagttt attggggcga
      961 tgtcggttcg gttttagaac gtagagctcg ccattgggat gtcaaaaccc aagaaatcaa
     1021 cttagataac caattagaat gggtatttag cacagcaaat ataaatcata ccttgttagc
     1081 cggtattgat tataaacgag tgcgtaaaga tcttgataac tatatgggga ttgcccctga
     1141 ttttgattgg cagaaccctg tttatggcgt gaccgttact gagcctagct tattttcaag
     1201 tgaagtgaag catttaaaac aacttggggt ttatctacaa gatcaaatag aaatcggtaa
     1261 ttttgacttc ctgttaggcg gtcgttacga tcgagcaaaa aacagcaacc aagatcgttt
     1321 gaacagtaaa acagagaata accaagaagg aaaatttact tggcgtacag gcgtagttta
     1381 caactttgaa aacggtatcg ctccgtatat cagctacagc acttcttttg tgccatcggc
     1441 tgaaaaagat gctcaaggaa atttcttaaa accaacaact gctgggcaat gggaagtggg
     1501 aatgaaatac caacctaccc aagacacctt actgacctta gcaggattta aaattacaca
     1561 aaaagattta gccaattatc aatggcaaac ccgctcttat gagcaaattg gtgaagtgca
     1621 aaccaaagga attgaagtga gtttaaatca gcaattatcc gataaatttt caatagcggc
     1681 ttcttatgct tacttaaaaa aagagattac tgcagataaa gatggcacga cgattggaaa
     1741 aacgcagtgg ggagtgcctc gccatcaagc atctctttgg gcaaaatata aattctttga
     1801 taagctgacc gccggtgtag gtgtacgcta tatgggaacg acttttggcg ataaccaaaa
     1861 tacttttacc gtaccggcat acacacttta cgatatgatg ctagcttatg attttatgcc
     1921 taacttaaac ttacagctca acgcacaaaa cctgacgaat aaaaaatatg tcgcaagctg
     1981 tgccaataat ttttcctgtt tctatggaaa agatcgcaat ttaagtgcaa cgttaaacta
     2041 tcatttttaa tctcaacagc cctcaaatga gggcttaaac ttttgcaaaa aatatgaaaa
     2101 aaacgaccgc ttgtattctt acttttttgg tttcatctgt gctttatgct aacaatataa
     2161 ccattgagct taaacagaat gattttgtgc aaggcaaatt gattcagaag gctcaacagc
     2221 cactttcctt gtcgattatt tttcctgacc aaagctctcg cctgctggct caaaatgtct
     2281 ttggtgaaca agattttatg tttaaatcag aactaatcgg cacagctact ttttctattc
     2341 tagcgaatgg tgcaaccgtt tcagaccaag attttacctt aacgattgag cggcatattc
     2401 caatctcggc acaaattgct ccacctaaag taattgaaaa tacacagctt gaagaactca
     2461 gccaaaaaat tgcaaaaaat ccagaaaaac agaccgcttt aattaacgat ttttgggata
     2521 aagcaaaaca agccggcaca ccattgattg aaccattgga cgaacagcaa agccgtgtaa
     2581 cttttttatg gcgtggagca acgaaaaatg cccgtatttg gggcggtgtg agtactgaac
     2641 acgattttat gcaacgcttt ggtgaaacgg atatatggta taaaagttat atagtgccca
     2701 atgacacttt agtggaatac aaattagctc cagatgtgcc gacattgccc gttgatgaga
     2761 atacccaacg cagagcgtta ctggctacgg ctcaagccga tccactcaat aaaacagcat
     2821 acttcgagaa aatcggacaa aatattcata ataccgataa attcaactac gcttcgctac
     2881 ttaaattgcc aaacgcacct actcaatctt ttttgactga agctccaaat attgcaaaag
     2941 gtaaactgca gcagttgatt tttgagagtg caacactcgg caataaacac agattattcg
     3001 tctatctgcc tgacggtttt agtgcagaaa aaaactatgc ggttctctat ttatttgatg
     3061 gtgttgaata taccgaacga gtgccaacta aaaccatttt agataatatg atcgctcaga
     3121 aagtgattct gcctactgta gcggtgttta tcgaaaatcc atcgagtgaa tcaagacgtg
     3181 tcgagctgcc tgccaatccc atatttacgc aagtattggc aaaagaactt gtaccttttg
     3241 ttgaaaaaca gctcaatata aaaacaaagg agcgaattat tggcggctct agctatggcg
     3301 ggctatcctc tgcttacacc gtactcaact atcctaatgt atttagtaaa gcattgattc
     3361 tatccggttc attttggtgg caacccaaag gcacacctag tgaacaaagc cactatattg
     3421 ctcaacaatt tatccaaaaa gaaaaattac cactctgttt tttcatcagt gcaggttttt
     3481 atgaaagcgg gaaatcggct atttgtaagc ggtcgaatta ttttgcatag aattacactc
     3541 aattttgcat agataaaaat taaaaactat gcaaaataat tgtagaagat caaacctaat
     3601 ctgacagtcc cccgttttaa attaccgtgt ctgtcagatt aatttgagct taaattcttt
     3661 tccaccc
//
LOCUS       DANLUQ010000186         3610 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.187, whole genome shotgun sequence.
ACCESSION   DANLUQ010000186 DANLUQ010000000
VERSION     DANLUQ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.187"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(78..200)
                     /locus_tag="PXB27_002357"
     CDS             complement(78..200)
                     /locus_tag="PXB27_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HDL5347098.1"
                     /translation="MKKLVLTILAAATFLTACNTVDGVGEDVEKAGDKIQQAAQ"
     gene            complement(280..1257)
                     /gene="rluD"
                     /locus_tag="PXB27_002358"
     CDS             complement(280..1257)
                     /gene="rluD"
                     /locus_tag="PXB27_002358"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018652666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HDL5347099.1"
                     /translation="MTQQKILTAEITADLLGARLDQALAQLFPDYSRSRIKVWIESDL
                     VKVNGNIVNKAREKVFGGETVEITAEIEEEIRFEPQDIPLNIVYEDDDIIVINKPKDL
                     VVHPGAGNPDGTVLNALLHYYPPIAEVPRAGIVHRLDKDTTGLMVVAKNIPAQTHLVT
                     ALQKRRITREYEAIASGIMTQGGKVDEPMARHPTKRTAMAVHPMGKPAVTHYRIMERF
                     RNYTRLRLRLETGRTHQIRVHMAHIAHPLLGDQLYGGRPRPPKGASDSFLSVLRGFQR
                     QALHATMLRLEHPITGEMMEWHAPLPDDFIELIQALKADYELYKDQLDY"
     gene            1398..2177
                     /locus_tag="PXB27_002359"
     CDS             1398..2177
                     /locus_tag="PXB27_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HDL5347100.1"
                     /translation="MRKFKSLATLVLAGLFVAGCSNSNKELEQSNVQDLYGKGQTYLQ
                     DGDYNSAIRYLEAVGTKGGQYSGLGEQTQLSLIYAQYKVGEYYKALDAAERFARSYPN
                     AANMDYVFYLAGLSNARLSDNFIQDFFGVNRASRAVDNVRNAYGNFQTIVQRYPQSQY
                     AQDAQNWMNYLFNRLAEHELAVVKFYDERNAYVAVVNRIEEMLRFYPNTLATKQALPY
                     LKEAYKQMNIPDAEQKTELLIQEYESKELPNPEKLKYGEQF"
     gene            2250..3281
                     /gene="tsaD"
                     /locus_tag="PXB27_002360"
     CDS             2250..3281
                     /gene="tsaD"
                     /locus_tag="PXB27_002360"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDL5347101.1"
                     /translation="MRILGIETSCDETGVAIYDEDKGLVANQLYSQIDMHADYGGVVP
                     ELASRDHIRKTLPLIQEALKEANLQPSDIDGIAYTAGPGLVGALLVGSTIARSLAYAW
                     NVPALGVHHMEGHLLAPMLEENAPEFPFVALLISGGHTQLVKVDGVGQYELLGESIDD
                     AAGEAFDKTGKLLGLDYPAGVAMSKLAESGTPNRFKFPRPMTDRPGLDFSFSGLKTFA
                     ANTIKANLNENGELDEQTKCDIAHAFQQAVVDTILIKCKRALEQTGYKRLVMAGGVSA
                     NKQLRADLAEMMKKLKGEVFYPRPQFCTDNGAMIAYTGFLRLKNDEQTDLSISVKPRW
                     AMTELPPIN"
     gene            3363..>3610
                     /locus_tag="PXB27_002361"
     CDS             3363..>3610
                     /locus_tag="PXB27_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HDL5347102.1"
                     /translation="MNNYVISLTSAQERRKHIEAEFGKQNIPFQFFDAITPDLIKEKA
                     KAFNIDISNTNLTKGEIACALSHIALWHLAKQQNLDYI"
BASE COUNT         1097 a          758 c          697 g         1058 t
ORIGIN      
        1 cgcaaaaata taccttattg aacaattgtt caataaggta tatatattat tagcttctta
       61 gaattcgaag ctttttgtta ttgtgcagcc tgctgaattt tatcgcccgc tttttcaaca
      121 tcctcaccaa caccatcaac ggtattacac gcagtcaaga aagttgcagc tgctaaaata
      181 gttaatacta attttttcat agaaagctcc ttttttgttg tttacttatg aaaacgattt
      241 caatatagca actttacatt atttaagcaa cacctaaact taataatcta attgatcttt
      301 ataaagctca taatccgctt ttagtgcctg aatcaattca ataaaatcat caggtaacgg
      361 ggcgtgccac tccatcattt caccagtaat cggatgctct aatctcaaca ttgtggcgtg
      421 taaggcttgg cgttggaagc ctcgtaacac cgataaaaac gagtcactcg cacctttagg
      481 tgggcgaggt ctaccgccat agagctgatc acctaataat gggtgagcaa tatgcgccat
      541 atgtacccga atttggtgag tacgccctgt ttctaatctt aaacgtaaac gtgtgtaatt
      601 acgaaaacgc tccataatac gataatgcgt aaccgccggt tttcccattg ggtgaacagc
      661 cattgccgta cgtttagttg ggtggcgagc cattggctca tcaacttttc cgccctgtgt
      721 cataatgccg ctggcaatcg cttcatattc acgggtaata cgacgttttt gtaatgcggt
      781 tactaagtga gtctgtgccg gaatgttttt agccactacc attaagcctg tcgtgtcttt
      841 atccaatcgg tgaacaatcc ctgctcgagg cacttctgca atcggtggat aataatgtag
      901 caaagcattc agcactgtac cgtccggatt accagcccct gggtgaacca ctaaatcctt
      961 cggtttatta atgacgataa tatcgtcatc ttcatacaca atatttagag gaatatcttg
     1021 aggctcaaaa cgaatttcct cttctatttc agcggtaatt tcaacagttt caccaccaaa
     1081 tactttttcc cttgctttat tcacgatatt gccattaact ttcactagat cactttcaat
     1141 ccacacttta atgcgtgaac gagaatagtc cggaaaaagt tgtgccagag cctgatctag
     1201 gcgagcacct agtaaatctg cagtaatttc tgctgttaaa atcttttgtt gagtcattaa
     1261 accataaatc ctaattagtc atttgcttac ttgcgtgatg ttatatagaa tagcgagatg
     1321 aacttcataa gttattctta gtcagaggac attgtacatt aacttttcgt cctagacact
     1381 ttttataggt aaaacatatg cgtaaattta aatctcttgc cacattagta ttagccggac
     1441 tgtttgttgc aggttgttca aattcaaata aagaattaga acaatccaac gttcaagatc
     1501 tttatggcaa aggtcagacc tatttacaag atggagatta taattctgcc attcgctact
     1561 tagaagcggt tggaaccaaa ggaggccaat attcaggact tggtgagcaa acccaactaa
     1621 gtttaattta tgctcaatat aaagttggcg aatactataa agcacttgat gccgcagaac
     1681 gttttgctcg ctcttacccg aatgcagcta atatggatta cgtattctat ctagcaggat
     1741 tatcaaatgc acgtctaagt gataatttta ttcaagactt ctttggtgta aatcgcgcct
     1801 ctcgagccgt agataatgtt cgtaatgctt acggtaactt ccaaactatt gtacagcgct
     1861 accctcaaag ccaatatgca caagatgctc aaaattggat gaattactta tttaaccgtt
     1921 tagcggaaca cgaattagcc gttgttaaat tttatgatga acgtaatgcc tatgtggctg
     1981 tagtaaaccg tatcgaagaa atgttgcgtt tttacccgaa tacactggca accaaacaag
     2041 cattgccata cttaaaagaa gcatataagc aaatgaatat cccagatgca gagcaaaaaa
     2101 cggaactctt gatccaagaa tatgaaagca aagagctacc gaatcctgaa aaactgaagt
     2161 atggcgaaca attctagtcg tacagagaat aatgtgaggg gcgttcttcg ccccttttgg
     2221 ttttctaact tattttgact tctccaacta tgcgaatttt aggtattgaa acctcttgtg
     2281 atgaaaccgg tgttgccatt tatgatgaag acaaaggctt agtggcaaac cagctttata
     2341 gccaaattga tatgcacgcc gattacggtg gcgtagtccc tgaactggct tctcgagacc
     2401 atatccgtaa aacgttgcca ctaattcaag aagccttaaa agaggccaat ctgcaaccct
     2461 cggatattga cggcattgcc tatactgccg gcccaggctt ggtcggggct ttattggtcg
     2521 gctcaaccat tgcccgttcg ctggcttatg cttggaatgt tccggcattg ggcgttcacc
     2581 atatggaagg gcatttactt gccccaatgt tggaagaaaa tgcccctgaa tttccgtttg
     2641 tggcattatt gatttcaggt ggacacaccc aactggtaaa agttgacggc gttgggcaat
     2701 acgaactact cggggaatca attgatgatg ctgccggtga agcctttgac aaaacaggca
     2761 aactactcgg tttggattac cctgccggtg tagcgatgtc aaaattagcc gaatccggca
     2821 cgccaaatcg ttttaaattc cctcgtccaa tgaccgacag accgggactg gatttcagtt
     2881 tctccggttt aaaaaccttt gctgcgaata cgattaaagc caatcttaat gaaaatggtg
     2941 aactcgatga gcaaaccaaa tgcgatattg cccacgcatt ccaacaagcc gtggttgata
     3001 ctattttaat taaatgcaag cgagcgttag agcaaaccgg ctataaacgc ttagtaatgg
     3061 caggcggcgt aagtgccaat aaacaattac gagcagacct tgcggaaatg atgaaaaaat
     3121 taaaaggcga agtattctac cctcgcccac aattttgcac tgacaacggc gcaatgattg
     3181 cctacactgg ctttcttcgc ttaaaaaacg atgaacaaac cgacttaagc attagcgtaa
     3241 aaccccgctg ggctatgacc gaattaccac cgattaatta acctttcaag cggtgaaatt
     3301 tcttgttaat tttgcaaaaa tttaatcaaa aataaccgct tgctatatga tagattaaat
     3361 ttatgaataa ttatgtaatt agcctaacct ccgcacagga gcgtagaaaa catattgaag
     3421 ctgaattcgg caaacaaaat attccttttc aatttttcga tgcgattacg ccggatttaa
     3481 ttaaagaaaa agccaaagca tttaatattg acatttcaaa tacaaactta acaaaaggtg
     3541 aaatagcctg tgctttgagt catattgcat tatggcattt agccaaacaa cagaatttgg
     3601 attatatttg
//
LOCUS       DANLUQ010000187         3600 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.188, whole genome shotgun sequence.
ACCESSION   DANLUQ010000187 DANLUQ010000000
VERSION     DANLUQ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.188"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            56..361
                     /locus_tag="PXB27_002362"
     CDS             56..361
                     /locus_tag="PXB27_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDL5347103.1"
                     /translation="MKGLKAAYINEAAFRQEIWCATILFPLGLILGETNIEKALLVGT
                     VLLVLVTELLNSAVESVVDRIGADFHELSGRAKDIGSAAVFMAMVLLAITWILILFC"
     gene            398..2041
                     /locus_tag="PXB27_002363"
     CDS             398..2041
                     /locus_tag="PXB27_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HDL5347104.1"
                     /translation="MRLKKTLLSVALLSATSAMAYETGKTYRFTVLHTNDIHGHFWQS
                     DKGEYGLAAQKTLVDNIRKEVAEKGGSVLMLNAGDANTGVPESDMQNARPDIEGMNAI
                     GYDAMVLGNHEFDYPLQILSMQEKWAKFPFISANVINKKTDKPIVKPYVMLERGGLKI
                     AVIGLTTEDTAKLGNPDVTENAFFKNPIETAKNTLAEINQKEKPDVRIALTHMGYYFD
                     GKHGTNAPGDVTMARTLEKGAFDLIIGGHTHDTVCIDEKGQFKAKYTPGEECKPDFQN
                     GTWIVQAGEWGKFVGRADFEFKDGKATLVKYELIPVNLKKTIKKEDGTKEYRLYQPEI
                     KADEATYTLLKKYQDEGDKKLNIEVGRVKGLLDGKREHIRFQQTNLGRLIAQSQMERV
                     KADIGIMNSGGIRTSLEEGVITYKHILTVQPFGNMIATVDLKGQELLDTLTTIALKEV
                     DTGAYPQFAGISMVVDRTAKTISDVKIGGKPFDKEKTYKVSLPDYVAAGGDGYPLLKK
                     HPSYVNTGFIDAEMLKKFFEENKIIEADKYDPKNQIIFK"
     gene            complement(2248..3117)
                     /locus_tag="PXB27_002364"
     CDS             complement(2248..3117)
                     /locus_tag="PXB27_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL5347105.1"
                     /translation="MSSRGKILNTISSLQNSLTKTEKKIAAAILSQPELLSQCSLSEV
                     AKQLDVGEATFIRFCRTLGFKGYTDFKLELAIELATQNQDNRVLLDTDVSESDTSKDI
                     AEKLKVSLDNVIEETINLLDFNVLEKVVEELRKAKRIFLFGVGSSGLTAEDAKHKLMR
                     IGLQTDAVTNNHFMYMQAALVKEGDLVIGISHSGYSEEIVKSLRFSRANKATTVAITH
                     NLRSPVTEEANYVLINGNRQGHMQGDSIGTKMSQLFVLDLIYTLLVKAEPENALKQKQ
                     KTLNVILEQRVKG"
     gene            complement(3127..3591)
                     /locus_tag="PXB27_002365"
     CDS             complement(3127..3591)
                     /locus_tag="PXB27_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDL5347106.1"
                     /translation="MILADLSRDDYLKSLPPVFARLCKKLKSLDLVNLELGWQELENG
                     IRMNVMSFETTPAEGKKAELHRKFIDIQLLIDGEEMIEYGLSQPDLSKYDEYRDEDDY
                     QLTAEIDDKNNLILKPNMFAIFLPYEPHKPGNSVNGNKTLKKLVVKVPVELI"
BASE COUNT         1157 a          693 c          652 g         1098 t
ORIGIN      
        1 gaaaccaacc aataaagcag attttcaacg tgtgatcaat gcagctggct actctatgaa
       61 ggggttaaaa gccgcttata ttaatgaagc cgcctttcgc caagaaattt ggtgcgctac
      121 tattttattc cctctagggc ttattctagg cgaaaccaat attgaaaaag cattattagt
      181 aggcacagtt ttacttgttt tagttacaga actactcaat agtgcggttg aatctgttgt
      241 tgaccgcatt ggggctgatt tccacgagct ttctggtaga gctaaagata ttggctctgc
      301 agctgtattt atggcgatgg ttttactcgc tataacttgg atattaattt tgttttgtta
      361 actctatttt tcaaccaaca ctcttactga ggtatttatg cgcttaaaaa aaacattact
      421 ttcggtagca ctacttagtg caacatcagc aatggcctac gagacaggta aaacatatcg
      481 ttttacggtt ttacatacta acgatataca cggtcacttt tggcaaagtg ataaaggcga
      541 atacggtctc gctgctcaaa aaacattggt tgataatatt cgcaaagagg tcgcagaaaa
      601 aggtggttcg gtattaatgc tgaatgcagg tgatgcgaat acaggcgtgc cggagtcaga
      661 tatgcaaaat gcacgtcctg atattgaagg aatgaatgcg attggttacg atgcaatggt
      721 tctaggtaat cacgaatttg attacccact gcaaattctc tctatgcaag aaaaatgggc
      781 taaattcccc tttatttcgg ccaatgtcat taataaaaaa acggataaac ccattgtaaa
      841 accttacgtg atgttagaac gaggcggact aaaaattgct gttatcggat taacaaccga
      901 agataccgca aaattaggta atccagatgt aacggaaaat gcctttttca aaaaccctat
      961 tgaaacagca aaaaacacat tagctgaaat taatcaaaaa gaaaaacccg acgtacgtat
     1021 cgctttaacc catatgggtt actattttga cggtaaacat ggtactaatg caccaggtga
     1081 tgtaacaatg gcccgtacct tagaaaaagg agcgttcgat ttaatcattg gcggtcatac
     1141 ccacgatact gtttgtattg atgaaaaagg tcaattcaaa gccaaatata cgccgggtga
     1201 ggaatgtaaa ccagacttcc aaaacggtac ttggattgtg caagccggcg aatggggcaa
     1261 atttgttgga cgggctgatt ttgagtttaa agatggaaaa gctaccttgg taaaatatga
     1321 attaattccg gtcaacttaa aaaaaaccat taaaaaagaa gacggtacta aagaataccg
     1381 actttaccag cctgaaatca aagccgatga agcaacctat accttattga aaaaatatca
     1441 agatgagggc gataaaaagc ttaatattga agttggtcgt gtgaaagggt tattagacgg
     1501 caaacgtgag catattcgct tccaacaaac caatctcggt cgtttaattg cacaatcgca
     1561 aatggaaagg gtaaaagccg atatcgggat tatgaactcc ggcggtattc gtacctccct
     1621 tgaagaaggt gtaataactt ataaacatat tctgactgtt caacctttcg gtaatatgat
     1681 tgcaacggtg gatttaaaag gacaagaatt gttagatacc ctcaccacta ttgctctgaa
     1741 agaagttgat actggcgctt atcctcaatt tgccggcatt tcaatggtag tcgaccgtac
     1801 agcaaaaact atttctgatg ttaaaatcgg tggcaaacct tttgataaag agaaaactta
     1861 taaagtttct ctgcctgact atgttgcggc tggtggcgat ggctacccat tactgaaaaa
     1921 acaccctagc tatgtcaata caggctttat tgatgccgaa atgctgaaaa aattcttcga
     1981 agaaaataaa attattgagg cagataaata cgatccaaaa aaccagatca ttttcaaata
     2041 gagctataga aaatgaaact cactctggca gtctctgctc aaaagagctg tggctgcctg
     2101 tagattgttt tcttactttc aagtgttata ggaaatcgat aaaaaatccc taccaaatta
     2161 attcatcacc atatgatgaa aattaaaaaa cctctggcaa tactttgcca aaagtctttg
     2221 acttttatag ggtaatatca ctgcttatta gcctttaaca cgttgttcca aaatcacatt
     2281 cagtgttttt tgtttctgtt tcaacgcatt ttcgggttcg gctttaacta gtaaggtata
     2341 aattaaatca agaacgaaaa gttgtgacat tttagtaccg atagaatctc cttgcatatg
     2401 cccctgtcga ttaccattaa ttaacacata attagcctct tccgtgacgg gggaacgtaa
     2461 attgtgtgta atcgcaaccg ttgtcgcttt atttgcacga gaaaaacgta gtgatttcac
     2521 aatttcttca gaatagcctg aatgactaat ccctataact aaatccccct ctttcaccaa
     2581 tgccgcctgc atatacataa aatgattatt tgtgactgca tcggtttgta gcccaatacg
     2641 cattaattta tgttttgcat cttctgcggt taacccagaa gaacccactc caaataaaaa
     2701 gatccgtttt gccttacgta attcttcaac aactttttcc aacacgttaa agtcaagcaa
     2761 attaatagtc tcttcaataa cgttatctaa actcactttg agcttctcag caatatcttt
     2821 cgatgtatcc gattctgaaa catccgtatc aagcaatact ctattatctt gattttgcgt
     2881 agccaactct attgcaagct ctaatttaaa gtcggtataa cctttaaaac ctaatgtacg
     2941 acaaaaacga ataaaagttg cttcaccaac atctaattgt ttggccactt cagataatga
     3001 acattggcta agtaattccg gctgagataa aatagcagca gctattttct tctctgtttt
     3061 ggttaaacta ttttgtagcg agctaattgt attaagaatt tttccccgac tagacatagt
     3121 agcctcctaa atcaattcaa caggtacttt caccaccagt tttttcaatg ttttgttgcc
     3181 attcaccgaa tttccaggct tatgtggttc ataagggagg aaaatagcga acatatttgg
     3241 cttgaggatc aagttatttt tatcatcaat ctcagctgtt aattggtagt catcttcatc
     3301 tcgatattca tcatacttag ataaatctgg ctggcttaac ccgtattcaa tcatttcttc
     3361 gccgtctatc aataattgaa tatcgataaa ttttcggtgt aactctgctt ttttcccctc
     3421 agctggcgta gtttcaaatg acattacatt catgcgaata ccattttcta attcttgcca
     3481 gcctaattcc aaattaacta aatctaaact ttttaatttt ttacaaaggc gagcgaatac
     3541 tggtggaaga gattttaaat aatcatctct tgataaatcg gctaatatca tataattctc
//
LOCUS       DANLUQ010000188         3514 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.189, whole genome shotgun sequence.
ACCESSION   DANLUQ010000188 DANLUQ010000000
VERSION     DANLUQ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.189"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            58..1860
                     /gene="frdA"
                     /locus_tag="PXB27_002366"
     CDS             58..1860
                     /gene="frdA"
                     /locus_tag="PXB27_002366"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HDL5347107.1"
                     /translation="MQSVNFDVAIIGAGGGGLRAAIAAAEANPNLKIALISKVYPMRS
                     HTVAAEGGSAAVIKDTDSFDNHFNDTVGGGDWLCEQDIVEYFVEHSPIEMTQLERWGC
                     PWSRREDGEVNVRRFGGMKIERTWFAADKTGFHILHTLFQTSIKYPNIVRFDEHFVLD
                     ILTDNGEARGCVAMNMMEGTLVQINANAVVIATGGGCRTYRFNTNGGIVTGDGLSMAY
                     RHGVALRDMEFVQYHPTGLPNTGILMTEGCRGEGGILVNKDGYRYLQDYGLGPETPIG
                     KPENKYMELGPRDKVSQAFWQEWRKGNTLKTAKGVDVVHLDLRHLGEKYLLERLPFIC
                     ELAKAYEGVDPAKAPIPVRPVVHYTMGGIEVDMNAETSIKGLFAVGECASSGLHGANR
                     LGSNSLAELVVFGKVAGENAARRALEVTARNQPQIDAQAKDVVARLHALANQEGNESW
                     SDIRNQMGDAMEEGCGIYRTQESMEATVNKIQELKERYKNISVKDKSSVFNTDLLYKI
                     ELGFILDVAQSIACSAVERKESRGAHQRLDYVERDDVNYLKHTQAYYNAEGTPTIKYS
                     DVKITKSQPAKRVYGAEAEAQEKVKKAAEAQAQQ"
     gene            1876..2607
                     /locus_tag="PXB27_002367"
     CDS             1876..2607
                     /locus_tag="PXB27_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005639269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HDL5347108.1"
                     /translation="MANLDKMTIEVLRYNPESDSEPHLDRYEVPYDSQTSLLDALGFI
                     KDELEPELSYRWSCRMAICGSCGMMVNGKPKLACKTFLRDYSGFMRIEPLANFPIERD
                     LVVDLSHFIDSIEAIKPYIIDNKAPEGQRTKQTPAQLEKYRQFSMCINCGLCYAACPQ
                     FGLNPEFIGPAAITLAHRYNLDNRDNGREERMKLLSSKNGVWSCTFVGYCSEVCPKHV
                     GPASAVNQGKLESAKDYVISMIKPR"
     gene            2617..3009
                     /gene="frdC"
                     /locus_tag="PXB27_002368"
     CDS             2617..3009
                     /gene="frdC"
                     /locus_tag="PXB27_002368"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HDL5347109.1"
                     /translation="MSATKRRPYVRGMKASWWKKLDFYKMYMVREATCLPTVWFCIVL
                     FYGAVALSKGTFTTDFVDFLQNPLVVILNIISLAAMLYHAATLFVMTPAVMSVMVKGK
                     HLPVSVGRNILWAATGVISVIALILVYI"
     gene            3023..3370
                     /gene="frdD"
                     /locus_tag="PXB27_002369"
     CDS             3023..3370
                     /gene="frdD"
                     /locus_tag="PXB27_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HDL5347110.1"
                     /translation="MSLDQNPKRSNEPPVWLLFSAGGMVSALFFPVVIFIIGLLLPFG
                     LVSPDNIISFAHTWIGKLAIMVLAIFPMWAGMHRIHHGLHDLKVHTPAGNVIFYGLSI
                     LYTILVIIAVVQI"
BASE COUNT         1031 a          642 c          841 g         1000 t
ORIGIN      
        1 gtggcacaat atgattactt tttaatgaat taagttattt aactttcgga gtgcattatg
       61 caaagtgtta attttgatgt cgcgattata ggtgccggtg gtggtggctt gcgtgctgct
      121 attgcggcgg ctgaagcaaa tccaaatcta aaaattgctt taatttcaaa agtttaccca
      181 atgcgtagcc atacggttgc agcagaaggt ggttcagcgg cggtaattaa agataccgac
      241 tctttcgaca atcactttaa tgataccgtc ggtggtggtg actggttatg tgagcaagat
      301 attgtggagt attttgtaga gcattcgccg attgaaatga cacaattaga acgctggggt
      361 tgcccttgga gtcgtcgtga agacggtgaa gtgaatgttc gccgttttgg aggaatgaaa
      421 attgagcgta cttggtttgc ggcggataaa accggtttcc acattttgca tactttgttc
      481 caaacttcga ttaaatatcc gaatattgtg cgttttgatg agcattttgt gctggatatt
      541 ctcaccgaca acggcgaagc tcgtggttgt gtggcaatga atatgatgga aggcacactt
      601 gttcaaatca atgcaaatgc agttgtgatt gcaaccggtg gcggctgccg tacttatcgc
      661 tttaacacta acggcggtat tgtaaccggc gacggtttat caatggcgta tcgtcacggt
      721 gtggctttgc gtgatatgga gttcgtacaa tatcacccaa caggtttgcc aaataccggt
      781 attttgatga cagaaggttg tcgtggtgaa ggtggtattt tagttaataa agatggctac
      841 cgttacctgc aagattatgg tttaggaccg gaaacaccta tcggcaaacc ggaaaacaaa
      901 tatatggaat taggccctcg tgataaggtt tcacaagcat tctggcaaga atggcgtaaa
      961 gggaacacct taaaaaccgc aaaaggcgtg gacgtagttc acttagattt acgccattta
     1021 ggtgaaaaat atttacttga gcgtttaccg tttatttgtg aattagcgaa agcctatgaa
     1081 ggggtcgatc cggctaaagc gccgattcct gttcgcccgg tggttcacta cacgatgggt
     1141 ggtattgaag tggatatgaa tgcggaaacc tcaattaaag gcttattcgc agtaggtgaa
     1201 tgtgcttcat caggcttgca cggtgcgaac cgtttgggtt ctaactcatt agcagagctt
     1261 gtggtgttcg gcaaagttgc cggtgaaaat gcggctcgcc gtgcgttaga agtgactgct
     1321 cgcaaccagc cacaaattga tgcacaagca aaagatgtgg tggctcgctt gcacgcatta
     1381 gcgaaccaag agggtaatga atcttggtcg gatatccgta accaaatggg tgatgcgatg
     1441 gaagaaggtt gtggtattta ccgtacccaa gaaagtatgg aagcgactgt aaacaaaatc
     1501 caagagctaa aagagcgtta taaaaacatc agcgtaaaag ataaatcaag tgtgtttaac
     1561 actgatttac tctacaaaat tgaattaggc ttcattttag atgtggctca atccattgct
     1621 tgctctgcgg tagagcgtaa agaatctcgt ggggctcacc aacgtttaga ctatgttgag
     1681 cgtgatgatg tgaactactt aaaacacaca caagcgtact acaatgcaga gggtacaccg
     1741 acgattaaat acagcgatgt gaaaatcact aaatcacaac cggctaaacg tgtgtatggt
     1801 gcagaggctg aagcccaaga aaaagtcaaa aaagcagcag aagctcaagc acaacaatag
     1861 agaaggagta gtaaaatggc aaatttagat aaaatgacca tcgaagtgct acgctacaac
     1921 cctgaatcag atagcgagcc acatttagac agatatgaag tgccgtatga cagccaaact
     1981 tcacttttag atgcattggg cttcattaaa gacgaattag aaccggaact ttcttatcgc
     2041 tggtcttgcc gtatggcgat ttgtggttct tgcggaatga tggtaaacgg taagccgaag
     2101 ttagcttgta aaacattcct gcgtgattac agcggtttta tgcgaattga gccgctggcg
     2161 aacttcccga ttgagcgtga cttagtagtg gatttaagcc actttattga tagcatcgag
     2221 gcaattaaac cttatattat tgataataaa gcaccggaag gacagcgtac taaacaaacg
     2281 ccggcacaat tagagaaata tcgtcaattc tcaatgtgta tcaactgcgg tttatgttat
     2341 gcggcttgcc cacaatttgg tttgaaccct gaatttatcg gacctgctgc gattacattg
     2401 gctcaccgct ataacttgga taaccgtgat aatggtcgtg aagagcgtat gaagctactc
     2461 agcagcaaaa acggtgtttg gagctgtact ttcgtgggtt actgctcgga agtctgtccg
     2521 aaacacgtcg gccctgcttc ggctgtaaac caaggtaaac ttgaaagtgc gaaagattat
     2581 gttatctcaa tgatcaaacc gagataggag gaagaaatgt cagcaacaaa acgtagacct
     2641 tatgtaaggg gaatgaaagc cagctggtgg aaaaaactcg acttctataa aatgtatatg
     2701 gtgcgtgaag ctacctgtct tccaactgtt tggttctgca ttgtattatt ttatggagca
     2761 gttgcgttaa gtaaaggaac atttactacc gattttgtgg atttcttaca aaatccgtta
     2821 gttgttatcc taaacataat ttcattggca gcaatgttat atcatgcggc aacacttttt
     2881 gtgatgacac ctgccgtcat gtccgtgatg gttaaaggta aacacttacc agtctcggtt
     2941 ggaagaaata ttctttgggc agcaacaggc gtaattagcg taattgcatt aatcttggtt
     3001 tatatttaag aaggggaaga aaatgagtct tgatcaaaac ccaaaacgtt caaatgaacc
     3061 gccggtatgg ttgcttttta gtgctggggg aatggtaagt gcattatttt tccctgtggt
     3121 aatctttatt atcggtttat tactgccatt cgggttagta tcaccagata atatcatttc
     3181 ctttgcccat acttggatcg gcaagctcgc tattatggta ttagcgattt tccctatgtg
     3241 ggcaggaatg caccgtatcc atcatggatt acacgacctg aaagttcaca cgccagcagg
     3301 taatgtgatt ttctacggat tgtctatctt atacacaatt ctagtaatca tcgcagtcgt
     3361 gcagatttaa atgagaaaga ccatcttgta taaagcagat ggtcttttca tttttaatat
     3421 gctaacaagc ggtcgaattt ataagaaaat ttgcaaatat tagaaatttt gccgataaaa
     3481 cgatcgtttt gtattaaata tctgcaaaaa agat
//
LOCUS       DANLUQ010000189         3455 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.190, whole genome shotgun sequence.
ACCESSION   DANLUQ010000189 DANLUQ010000000
VERSION     DANLUQ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.190"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            33..1115
                     /gene="aroG"
                     /locus_tag="PXB27_002370"
     CDS             33..1115
                     /gene="aroG"
                     /locus_tag="PXB27_002370"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HDL5347111.1"
                     /translation="MSYIKNDDVRITSIEELLPPIALLEAYPASDVAAETVAKTRQSI
                     HKILHGADDRLLVVIGPCSIHDPKAALEYAQKIKAMRADPKINQNLEVVMRVYFEKPR
                     TTVGWKGLINDPYLNETFALNDGLRIARKVLSDINDLSVPTAGEFLDMITPQYVADFM
                     SWGAIGARTTESQVHRELASGLSCAVGFKNGTNGSVKIALDAISSAKSPHHFLSVTKF
                     GHSAIVSTAGNPDCHIILRGGDSATNYDADSVAAACAAIEKSGGRPHVMIDFSHANSQ
                     KQFKRQMDVCADVCNQIAGGSDFISGVMIESHLVEGRQDLIEGKGLEALVYGQSITDG
                     CIGWEDSEKALFALAEAVEKRRNFAK"
     gene            1202..1894
                     /gene="tsaB"
                     /locus_tag="PXB27_002371"
     CDS             1202..1894
                     /gene="tsaB"
                     /locus_tag="PXB27_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDL5347112.1"
                     /translation="MKPSILALDTATEACSVALQYNGNITFLDEISPRSHTQRILPMV
                     DELLNQANIELKEVNYLVFGRGPGSFTGVRVGVSVAQGLAMGANLPVVAVSNLKAMAE
                     EAYQKLGATKVIALIDARMNEVYFAQFERKGEEWNEVVAEQVCSPEVAISQFQMSEET
                     VVVGTGWAAYSQFSEQNLPLVISDITLPSSRYMLSIAETEIQKGNIQSAFEIEPVYLR
                     NEVTWEKLPHKQ"
     gene            complement(1911..2720)
                     /locus_tag="PXB27_002372"
     CDS             complement(1911..2720)
                     /locus_tag="PXB27_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDL5347113.1"
                     /translation="MTMTVIIILIICGIIVNITSALFGIGGGVLMMPILHSLFPELPL
                     QVISACSLTIVIGSATINLCLFHHQQIKINRKNVLIWSLAMMLGVQIGFELSFLFSDN
                     SIIAIFILTLLTLSFKTLFHKPRVKAIVPPKPKEAVSNATMCSLGGLIAGITGIGGGS
                     IMAPIVTQIRSVSPKQVAVYTNYMMVIGGIGNLYGYLSRPFVLNSTLATWQIGYVNFA
                     IVGTVVISSLFTVYFSMKLRNILSPELTTKLLGVILFTIACYMGFLQYLFT"
     gene            2819..3370
                     /locus_tag="PXB27_002373"
     CDS             2819..3370
                     /locus_tag="PXB27_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL5347114.1"
                     /translation="MNNMPTPEQLMSCQLSVMSDLEKGVVIYSQGYHAREFYFLKSGL
                     IGLYHMLENGKESLVRVYKANDYFGFRTFFGDNRYHCSARVLQPAELIRITPLNFIEF
                     WSNNSALIISLLKQLSTELQDAEHRLSMVSYQKTQERVMNSIHYLHQRYPNYHWTNRE
                     IAEFAGCETETAIRLRRELKKIS"
BASE COUNT         1110 a          636 c          733 g          976 t
ORIGIN      
        1 tttattttca ttttatttag agagtgaaca caatgtctta tatcaaaaat gacgatgtca
       61 gaattaccag tattgaagaa ttactaccac ctattgcatt actggaagcc tatccggcaa
      121 gtgatgttgc agcggaaacc gttgctaaaa cccgtcaatc aatccacaaa attttacacg
      181 gtgcggatga tcgcttatta gtggtgattg gaccttgctc cattcacgac ccgaaagcgg
      241 cattagaata tgcacaaaaa attaaagcaa tgcgtgctga tccgaaaatt aaccaaaact
      301 tagaagtggt gatgcgtgtc tattttgaaa aaccccgtac aaccgttggt tggaaaggct
      361 taattaacga cccttatttg aacgaaacct ttgccttaaa cgatggttta cgcattgccc
      421 gtaaagtatt atctgatatt aatgatttaa gcgtgccgac tgccggcgag tttttggata
      481 tgattacacc acaatatgtg gctgacttta tgagctgggg agcaattggg gcgagaacga
      541 ctgaatctca agtccaccgt gaattagcct ctggtttatc ctgtgcggta ggttttaaaa
      601 acggcacgaa tggtagtgtg aaaattgcat tagatgcaat ttcttccgca aaatcgccac
      661 accatttctt atcggtcact aaattcgggc attctgcgat tgtttcaaca gcaggaaatc
      721 ctgactgtca cattattttg cgtggtggcg atagcgcaac caactacgat gctgactccg
      781 ttgcagcggc ttgtgctgcg attgagaaat ccggcggacg tccacacgtg atgatcgatt
      841 tcagccacgc taacagccag aagcagttca aacgccaaat ggacgtttgt gctgatgtgt
      901 gcaaccaaat tgcaggtgga tcagacttta tttcaggtgt gatgattgaa agccacttgg
      961 tagaaggtcg ccaagactta attgaaggca aaggtttaga ggcgttagtt tacggacaaa
     1021 gtattacaga cggctgcatt ggctgggaag atagtgaaaa agcgttattc gcacttgctg
     1081 aagcggttga aaagcgcaga aattttgcaa aataatcttt tcttgaaccg agggcgaata
     1141 taattcgccc tttatttgtt taaccttaaa gcaaatttaa tttattcaaa ctaaacatat
     1201 gatgaaaccc agcattcttg ctttagatac tgcaacagag gcttgctctg tagcgttaca
     1261 gtataacgga aatatcacgt tcttagatga aatcagccct cgttcccata ctcaacgtat
     1321 tttgccgatg gtagatgaat tacttaatca agccaatatc gaactcaaag aagtaaatta
     1381 tttagtattc ggacgtgggc cgggcagttt taccggtgtg agggtcggtg taagtgtggc
     1441 acaagggttg gcaatgggag cgaatttgcc ggtggtagct gtttctaact taaaagcaat
     1501 ggcagaagaa gcataccaaa aattaggtgc aacaaaggta attgccttaa ttgatgcccg
     1561 aatgaatgaa gtttactttg ctcaatttga acgaaagggt gaagaatgga atgaagtggt
     1621 ggctgaacaa gtctgttcgc cagaagtcgc aataagtcaa ttccaaatga gtgaagagac
     1681 tgtggtggtt ggcacaggtt gggcggcata ttcgcaattt tctgagcaaa atctaccgct
     1741 tgttatcagt gatattacct tgccttccag ccgatatatg ctttcgattg ctgaaactga
     1801 gattcaaaaa ggcaatatcc aatctgcttt tgaaatagaa ccggtgtatt tgcgtaatga
     1861 agttacttgg gaaaaactac ctcataagca ataatctatt gaacttatgg ttaagtgaat
     1921 aaatattgca aaaagcccat ataacaggca atagtgaata ggattacacc aagtaattta
     1981 gtagttaatt cgggactcaa gatattgcgt aatttcattg aaaaatagac agtaaagaga
     2041 gaacttatca ctacggtgcc gacaattgca aaattcacat agccgatttg ccaagttgct
     2101 aaggttgaat tcaacacaaa agggcgggat aagtagccat ataaattgcc gattccgcca
     2161 atcaccatca tatagttagt ataaactgca acctgtttcg gtgaaaccga gcgtatttgc
     2221 gtgacaatcg gagccataat cgaaccgccg ccaatgccgg taatacctgc aattaaaccg
     2281 cctaaactgc acattgtggc attagatact gcttcttttg gtttaggtgg tactattgct
     2341 ttaactcttg gtttgtgaaa tagggtttta aatgaaagtg tcagcagggt tagaataaag
     2401 atagcgataa tgctgttatc agagaaaaga aaactgagtt caaacccaat ttgtacgcct
     2461 aacatcatcg caagtgacca aatcaacaca ttttttcgat tgatttttat ctgttgatgg
     2521 tggaataagc ataaattgat agtggctgaa ccaatcacaa tggtaagaga gcaggcggaa
     2581 atcacttgga gaggcagttc aggaaacagt gaatgtaaaa tcggcatcat taaaacaccc
     2641 ccgccaatcc caaatagtgc agaagtgata ttgactataa tgccacaaat gatcaaaata
     2701 attatcacgg tcattgtcat aatccatctc ctaaagtgtg ataagtatgc aaaattttct
     2761 aaataaagga ttatgatcgc aatcatatat ggaaaaaata aaataataag gataatcaat
     2821 gaacaatatg ccaacccccg aacaattgat gtcttgccaa ttaagtgtaa tgtctgatct
     2881 agaaaaagga gtcgtcattt attctcaggg ttatcacgcc agagaatttt attttttaaa
     2941 aagcggatta atagggctgt accatatgct tgaaaatggt aaagagagct tggtgagggt
     3001 atataaagcc aacgattatt ttgggtttag aacctttttt ggcgataatc gctaccattg
     3061 ctcggctcgg gtgttacagc cggcagaatt gatacgtatt acccccttaa atttcattga
     3121 gttttggtcg aataattccg ccttaattat ctctctgtta aaacagctct cgacagaatt
     3181 acaagacgca gagcatcgtt tatcaatggt ttcctatcaa aaaacgcaag agcgggtaat
     3241 gaacagtatt cattatttac accaacgtta tcccaattat cactggacta accgtgaaat
     3301 cgccgaattt gcaggttgtg aaaccgaaac agccattcga ttaaggcgag aattaaagaa
     3361 aatcagttaa attgtcataa attattcata gttgtgatca ccatcacata tctattatga
     3421 caaacatcat attctatcgt taaaaataag attta
//
LOCUS       DANLUQ010000190         3454 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.191, whole genome shotgun sequence.
ACCESSION   DANLUQ010000190 DANLUQ010000000
VERSION     DANLUQ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.191"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            10..1944
                     /gene="cpdB"
                     /locus_tag="PXB27_002374"
     CDS             10..1944
                     /gene="cpdB"
                     /locus_tag="PXB27_002374"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005614893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HDL5347115.1"
                     /translation="MLVLGTQPSAFAQTAKNKAGLRVIATTDIHGFLTDFDYYKDAPT
                     EKFGFTRTASLIKQARAEVKNSVLVDNGDLIQGNPISDYQSTKGAKEGKPEPSIMALN
                     AMQYEVGTLGNHEFNYGLSYLDNAIKQAKFPIVNANIVKPGTDEPFFTPYVIQQKEIV
                     DEKGNKHTIKIGYIGFVPPQVMIWDKANLEGKVEARDIVKTAQKYVPIVKKEGADIVV
                     ALAHTGPSDEPYKEGAENCAFYLADVKGIDAVIFGHSHRLFPNKEFANSPNADIAKGT
                     MKGVPESMAGYWGNNISVIDLSLAENDGKWGVVDGRAELRPIYNTEEKKATVENHPEI
                     VALLKETHEATRQFVSQPIGKATDNMYSYLALVQDDPTIQIVNQAQKAYVENVVKGLP
                     ELAGLPILSAGAPFKAGGRKNDPTGYTEVDKGELTFRNAADLYLYPNTLVVVKATGAE
                     LKEWLECSAGMFKQIDVASDKPQYLLDWTGFRTYNYDVIDGVSYQFDITQPARYDGEC
                     KLINPNSHRVVNLTFDGKPVDPKQEFLIATNNYRAYGAKFPGTGDKHIVFAAPDENRQ
                     VLANYITTESKAHGQVSPKADNNWRIAPISSNAKLDIRFETSPTDKAKAFIKENAQYP
                     MEYIENDETGFAVYRIDLTK"
     gene            complement(2017..>3454)
                     /locus_tag="PXB27_002375"
     CDS             complement(2017..>3454)
                     /locus_tag="PXB27_002375"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDL5347116.1"
                     /translation="PSLLCDFYKTSHRIQYPEGSQFLYSTFTPRSNIYAPFVTRVVSF
                     GFQAFILKYLIHYFNDNFFSRSQHDVVSEYTQFISDTLNIEDSGEHIAQLHQLGYLPI
                     RIKAIPEGKSVAVKVPLMTIENTHSSFFWLTNYLETLINVSLWQPITSASIAFAYRKA
                     LAEFSEQTCDDNSHLPFQAHDFSMRGMSSLESAESSGAGHLTSFLGTDTIPAISFVTA
                     YYGSQHLIGTSIPASEHSVMSSHGVDELPTFRYLMAKYPNNMLSIVADTTDFWHNITV
                     NLPLLKQEILARPESAKLVIRPDSGDFFTIICGNNSSPVEHERKGLIECLWDIFGGSI
                     NQKGYKVLNPHIGAIYGDGVTYDKMIRILEGLTAKGFASSNIVFGVGAQTYQRNTRDT
                     LGFAIKATSITINGVEKAIFKAPKTDNGLKKSQKGRVKVISYDHYVDELTALDDFSDD
                     LLEVVFENGKLVKSTNFDEIRQNIAQQF"
BASE COUNT         1153 a          656 c          704 g          941 t
ORIGIN      
        1 gcaactgcaa tgcttgtatt ggggacacaa ccgtctgcat ttgcacaaac agccaaaaat
       61 aaagctggct tgcgtgtcat cgccactacg gatattcacg gtttcttaac tgattttgat
      121 tattacaaag atgctccaac agaaaagttt ggttttaccc gtacggcaag cctaattaaa
      181 caagctagag cagaagttaa aaacagtgtg ttagtcgata atggtgactt aattcaaggt
      241 aatcctattt ctgactacca gtcaaccaaa ggtgcaaaag agggcaaacc tgaaccttcg
      301 attatggcat taaatgcgat gcaatatgaa gtcggaacgc tgggaaatca tgaatttaac
      361 tacggtttaa gctatttaga taatgctatt aagcaagcaa aattccctat tgtcaatgca
      421 aacattgtga agccggggac agatgaacca ttctttacac cttatgttat tcaacaaaaa
      481 gaaattgtag atgaaaaagg gaataagcat accattaaga ttggttatat cggttttgta
      541 ccaccacaag tgatgatttg ggataaagct aatcttgaag gaaaagttga agctcgagat
      601 attgtgaaga ccgcacaaaa atatgtgcct atagtaaaaa aagaaggggc agatattgtc
      661 gttgcattgg cacataccgg cccttcagac gaaccctata aagaaggcgc tgagaactgt
      721 gcgttttatc tagctgatgt aaaaggtatt gatgcagtta ttttcgggca ttcacatcgc
      781 ctgttcccaa ataaagaatt tgcaaattca ccaaatgccg atattgcaaa aggaacaatg
      841 aaaggcgtac cggaaagtat ggctggctac tggggaaata acatcagtgt gattgattta
      901 agcttggccg aaaacgatgg taaatggggc gttgtggacg gtagagcaga gttgcgtcca
      961 atttataata cggaagagaa aaaagcaacc gttgagaacc accctgaaat cgttgcatta
     1021 ttaaaagaaa cccatgaagc aacccgtcaa tttgtgtctc aacctatcgg taaagcaact
     1081 gataatatgt atagttacct tgcgttagtt caagatgacc ctaccattca aattgtgaac
     1141 caagctcaaa aagcgtatgt agaaaatgtg gtaaaaggct tgccggagct tgcaggatta
     1201 ccaattctaa gtgccggagc accgtttaaa gccggtggac gtaaaaatga cccgacaggc
     1261 tataccgaag tcgataaagg cgaattaact ttccgcaatg cggcagactt atacctttac
     1321 ccaaatacct tagtggtggt taaagcaaca ggagcagaat taaaagagtg gttagagtgc
     1381 agtgcaggaa tgttcaaaca aattgatgtc gcttcggata aaccgcaata tttactggat
     1441 tggacaggtt tccgcaccta taactacgat gtaattgacg gtgtcagcta tcaatttgat
     1501 ataacccaac cggctcgtta tgacggcgag tgcaaattga tcaaccctaa ttcgcaccgt
     1561 gtggtgaact taacctttga cggcaaaccg gttgatccta aacaagaatt cttaattgcc
     1621 accaacaatt accgtgctta tggagcaaaa ttcccgggaa caggcgataa acatattgtg
     1681 ttcgccgcac cggacgaaaa ccgccaagtg ttagcgaatt atattaccac cgaaagtaaa
     1741 gctcacgggc aggtaagtcc gaaagcggat aacaactggc gtattgcacc aattagctca
     1801 aatgcaaaac tggatattcg ttttgaaacc tctccaacag ataaagctaa agctttcatt
     1861 aaggaaaatg cacaatatcc gatggagtat atcgaaaacg atgaaaccgg cttcgcagtt
     1921 taccgtattg atttaaccaa ataatcagag ctaaatacaa gcggtctaat ttgcaaaaaa
     1981 attaacaaat ttgaccgctt gtttcattcc atttttctaa aattgttgtg ctatattttg
     2041 ccgaatttca tcaaaattgg tcgatttgac caacttacca ttttcaaaaa ccacctctaa
     2101 aagatcatct gaaaaatcat ccagtgcggt tagctcgtca acataatgat cataagagat
     2161 tacttttact cgcccttttt gcgatttttt taagccgtta tcggttttag gagctttgaa
     2221 aattgccttt tcgacaccgt taattgtaat tgaagtcgct ttgattgcaa aacccagcgt
     2281 atcacgagta ttgcgttgat aagtttgagc cccaacgcca aacacaatat tgctggaagc
     2341 aaaacctttt gccgttaagc cctctaaaat ccgtatcatt ttatcataag tgacgccgtc
     2401 accataaatt gccccaatat gcggattaag cactttataa cctttttggt taatactacc
     2461 accaaaaata tcccacaaac actcaatcag acctttgcgt tcgtgttcaa cgggggagga
     2521 attgttaccg caaataattg taaaaaaatc accgctatca gggcggataa ccaattttgc
     2581 tgattccggt cgagctaaaa tttcttgttt taataatggc aaattaacgg tgatgttatg
     2641 ccagaaatcg gttgtatctg ccacaataga tagcatatta tttggatatt ttgccattaa
     2701 ataacggaaa gtcggcagct catcaacacc gtgtgaactc atcaccgaat gttctgatgc
     2761 cggaatagat gtaccgatta aatgttgaga accataatag gctgtcacga atgaaattgc
     2821 aggaatggta tctgtgccta aaaatgaggt tagatgtccg gcaccggagc tttctgctga
     2881 ttctaaggaa ctcattccac gcatagagaa atcgtgtgcc tggaagggta gatggctatt
     2941 atcatcacag gtttgctctg aaaattcggc tagagcctta cgataagcaa atgcaatgga
     3001 agctgatgta ataggctgcc ataatgacac attaatcagg gtttccaaat aattagtcag
     3061 ccaaaaaaag cttgagtgag tgttttcaat tgtcattaaa ggaactttca ctgcaacaga
     3121 ttttccttct ggaatagctt taattctgat aggtaagtaa ccgagctgat gcaattgtgc
     3181 aatatgttcg ccagagtcct caatattcaa tgtgtcagaa ataaattgag tatattcaga
     3241 aacaacgtca tgttgtgagc gagaaaaaaa attatcgtta aaataatgaa tcaagtattt
     3301 taaaataaat gcctgaaagc caaatgatac aacacgtgta acaaatggag cataaatatt
     3361 actacgaggt gtgaatgtac tatataaaaa ctgagaacct tcaggatatt gaattcgatg
     3421 tgaagtttta taaaaatcac ataatagtga tggt
//
LOCUS       DANLUQ010000191         3415 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.192, whole genome shotgun sequence.
ACCESSION   DANLUQ010000191 DANLUQ010000000
VERSION     DANLUQ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.192"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..1464)
                     /locus_tag="PXB27_002376"
     CDS             complement(<1..1464)
                     /locus_tag="PXB27_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HDL5347117.1"
                     /translation="MTLQIHSIRTKVKTPLAKRIAHALTDRKILQFRFDAHLRNQVWQ
                     RLTKTQKALLNRINAAGIDALPKRELDKLLKELKAEIAKNYQETTAYIQPELSGFFAV
                     EKQALTELYNDEIGYDFFNQVPSYQRKATQQATIIAGMPLESWFEKQGSELSFKFEST
                     IRQGLLEGRQTPEIAKATAELFGISRRHADTLVITAVAKVADDAHKALRDENLDLIKG
                     EMHLSTLDTRTSTVCQVRDGKMWDTDYKPIGHSLPYQRPPLHPRCRSILQLVMKDWKE
                     LGFDDVKEMPKSTRASADGQVSEKTNYEEWLSSKTPEEQDKALGKGKADLWRRGVITF
                     SDMLDQSARPLTLKELYSLKNSSSVDKMDSVELVRKKAVAVEPMITRDVVSIITASGG
                     EPAGLDFRLKSLSSLQRKIDTEIMAGVSKEQAIASIRDVIRYTAILDEQRFVEQYQKM
                     QKDLEKQGYSTIIVKNTWKSNNAYKGINTFVSTFIEKN"
     gene            complement(1412..2815)
                     /locus_tag="PXB27_002377"
     CDS             complement(1412..2815)
                     /locus_tag="PXB27_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4055 domain-containing protein"
                     /protein_id="HDL5347118.1"
                     /translation="MPNSISADFAALKAKTKIIDDLLGGTQKMRQAGQTYLYKMKMEE
                     EDGYKNRLNRSTLYPALRETLSQMLGRVFFNPINVEDVHKQIEPLFDDIDLEGNNLDV
                     FASRWFYAALAYGVSYCLVDFVRVENARSLAEEKAQNARPYLVHIKPQQVLGFKTAKI
                     NGKTAFTQFRYKETVTEDDGEFASKQVNYIYVYEIGRVRKYKNDEGGLSLVADMQIKA
                     QNTPLAFVPIVPFITKPIDVWGMGEPPLMELAYLNIKHWQSQSDQDNILNIARVPLLA
                     IFSDTQIKSLEIGSSAISLPKDSTMQFIEHSGSSINAGLTSLKELEDQMKTAGAKLLT
                     KTALAMTDSQAKDEAGKEISQLRHYANKFEDALDLAFEYIGHWLGIGVDEVGTSQISG
                     NIDNDLDPNASMATIIQLRNAGVISNHSTFEEAKRRGLLSDSAKWEDEQARLQAEGLS
                     DDFTNPFNSNQSENAAS"
     gene            complement(2828..>3415)
                     /locus_tag="PXB27_002378"
     CDS             complement(2828..>3415)
                     /locus_tag="PXB27_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL5347119.1"
                     /translation="GDYLESSDAQIFRDKYQELEFKPNPDFNGPYYGLDFGFANDPTA
                     CVKCWVFDDDLYIEREAGKVRLELDETAAYLANALPDLSNYTVRADSARPESISYLKR
                     HGLPYIQPVPKWKGSVEDGVEHIKSYRKVYIHPSCTEVLKEFRLYSYKTDRLSGDVLP
                     IIIDAHNHYIDAIRYALNPLIQARGEAKQCALKVY"
BASE COUNT          924 a          773 c          621 g         1097 t
ORIGIN      
        1 attcttttca ataaaagtgc tcacaaacgt attaatgccc ttataagcat tatttgattt
       61 ccaagtgttt ttaacaatga tcgtagaata accttgtttc tccaagtctt tttgcatttt
      121 ttggtattgc tcaacaaaac gctgttcatc taagattgca gtgtagcgaa taacatctcg
      181 aatacttgct atcgcttgct cttttgacac tccggccatt atttccgtat caatttttcg
      241 ctgcaaagat gatagtgatt tcaagcgaaa atctaagccg gcaggctcgc ccccagatgc
      301 tgtaataatt gatactacat cacgagtaat cataggctca actgcaacag cctttttacg
      361 gacaagttcc acactatcca ttttatctac cgaagaacta ttttttaatg aatatagctc
      421 ttttagtgtc aatggtctag cactttgatc taacatatcg ctaaacgtta tcactccccg
      481 ccgccacaaa tccgccttgc ctttgcctag cgctttatct tgctcttccg gtgttttgct
      541 actgagccac tcttcatagt ttgtcttctc cgacacttgc ccgtctgcac tcgccctagt
      601 gcttttcggc atttctttca catcatcaaa gcctaactct ttccagtctt tcatcaccag
      661 ttgcagaatc gaacgacagc gagggtgaag tggtgggcgt tgatacggca aactgtgtcc
      721 gataggcttg taatcagtgt cccacatctt accgtctcgc acttgacaaa cagtactggt
      781 gcgggtatcg agcgttgata agtgcatctc acccttaatt aaatcaagat tttcatctcg
      841 caatgccttg tgagcatcgt ccgccacttt tgccacagca gtaatgacaa gtgtatcagc
      901 gtgccgtctg gatataccaa ataattcagc cgtagctttg gcaatctcag gcgtttgcct
      961 accctctaac aagccttgcc gaatggtgct ttcaaattta aacgaaagct ctgatccttg
     1021 cttttcaaac caactttcaa gcggcattcc ggcaataata gtcgcttgtt gcgtagcttt
     1081 acgttgatat gacggcactt gattgaaaaa atcatagccg atttcatcat tgtaaagctc
     1141 agtcagtgcc tgtttttcaa ccgcaaaaaa accgcttaat tcaggctgaa tgtaagcggt
     1201 cgtttcttgg taattttttg caatttctgc tttgagttct ttgagcagtt tatccaactc
     1261 tcttttcggt aaggcatcta tcccggctgc attaattcgg tttaataatg ccttttgcgt
     1321 ttttgtgagt cgttgccaaa cttgatttcg taaatgagca tcaaaacgga actgcaatat
     1381 tttacgatct gttaatgcgt gagcgatccg tttagctagc ggcgttttca ctttggttcg
     1441 aattgaatgg atttgtaaag tcatcgctta aaccttccgc ttgtaacctt gcttgttcgt
     1501 cttcccattt tgcactatct gacaataaac ctcgtctttt agcttcttca aacgtagagt
     1561 gatttgaaat gacaccggca ttgcgtagct gaataatggt tgccattgac gcatttggat
     1621 ctaaatcatt gtcgatattg cctgaaattt gcgaagtccc aacttcatca acgccgatac
     1681 ctagccaatg cccaatgtac tcaaaagcca aatcgagagc atcttcaaat ttgttagcat
     1741 aatgtctaag ctgtgagatc tctttacctg cttcatcttt agcctgacta tccgtcattg
     1801 ctaatgctgt tttagttagc agtttcgcac cggcagtttt catttggtct tctagttctt
     1861 tcagactggt taaaccggca ttaatagacg aacctgaatg ctcaataaac tgcattgtac
     1921 tgtctttcgg cagagatatt gcacttgagc cgatttcaag ggattttatt tgagtatcag
     1981 agaaaatagc taatagcggt acacgagcaa tattcaaaat attgtcttga tctgactgac
     2041 tttgccagtg cttaatattt aaataagcca gctccattaa aggcggctca cccattcccc
     2101 aaacatcaat cggtttagtg ataaatggaa caataggcac aaatgcaagc ggtgtatttt
     2161 gagctttgat ttgcatatca gcaaccagtg ataagccacc ctcatcgttc ttatattttc
     2221 ggacacgtcc gatttcataa acataaatgt agttcacctg tttagaagca aactcgccat
     2281 catcttctgt aactgtctct ttataacgaa attgggtaaa agcagtttta ccgttgattt
     2341 tggctgtttt aaagcccagc acttgttgcg gcttaatatg caccaaatac ggacgtgcat
     2401 tttgagcttt ttcttctgca agactgcggg cattctctac cctcacaaaa tccaccaagc
     2461 aataagatac gccataagcc aatgcagcat aaaaccaacg agaagcgaat acatcaagat
     2521 tgttaccctc taaatcaata tcatcaaaca atggttcgat ttgtttatgc acatcctcta
     2581 cgttgatcgg attaaaaaac actctcccca acatctgcga aagcgtttcc ctcaatgccg
     2641 gataaagcgt agaacgattt aatcggtttt tatatccatc ttcctcttcc attttcattt
     2701 tatagaggta ggtttgaccc gcttgtcgca ttttttgtgt gccaccgagt aaatcatcga
     2761 tgatttttgt ttttgctttt aatgccgcaa aatcggctga gatggagtta ggcattgtta
     2821 cctcatttta ataaaccttt aatgcacatt gcttcgcctc gcctcttgcc tgaataagag
     2881 gatttaaggc ataacgaatg gcatcaatat agtggttatg agcgtcaata atgataggca
     2941 gtacatcacc acttaatctg tccgttttgt aactataaag gcgaaattct ttcaatacct
     3001 ctgtacaact tggatgaata tatactttac gataagattt tatatgctca accccatctt
     3061 cgactgagcc tttccacttt ggaacaggtt gaatataagg caatccatgt cgttttaaat
     3121 aactgatgga ttctgggcga gcagaatctg ctctaacagt ataattactc aaatcaggca
     3181 aagcatttgc taaataagca gcggtctcat cgagctctag tcttactttc cctgcttcac
     3241 gctcgatata taagtcatca tcgaacaccc aacatttcac acaggctgtt gggtcattgg
     3301 caaaaccaaa gtccagcccg taataaggac cgttaaaatc agggttaggc ttaaattcta
     3361 attcttggta tttatctcta aagatttgag catcagagga ttctaagtag tcccc
//
LOCUS       DANLUQ010000192         3388 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.193, whole genome shotgun sequence.
ACCESSION   DANLUQ010000192 DANLUQ010000000
VERSION     DANLUQ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.193"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..274)
                     /locus_tag="PXB27_002379"
     CDS             complement(<1..274)
                     /locus_tag="PXB27_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL5347120.1"
                     /translation="MIITMKDIAKEANVSLGTVSNVLNGKSNVSLAKIEKVQAAVEKL
                     GYRKNIQASTLKSGTSNKIAIILPNITELKYACLYENLDLICTQNDL"
     gene            complement(436..1776)
                     /gene="gdhA"
                     /locus_tag="PXB27_002380"
     CDS             complement(436..1776)
                     /gene="gdhA"
                     /locus_tag="PXB27_002380"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HDL5347121.1"
                     /translation="MSDLNALFQKIKQRDPNQAPFHQAVEEVFGSLAPFLAKNPKYTQ
                     QGLLGRIVEPERVISFRVTWVDDKGQVQVNRGYRVQMNSAIGPYKGGIRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMSELYRHIGADTD
                     VPAGDIGVGGREIGYMFGQYKKLRNEFTSVLTGKSLTWGGSLIRPEATGYGAVYFAES
                     MLATKGQQIEGKSVVISGSGNVAQYAAEKVIQKGGKVLTVSDSNGYVLFPESGMTEAQ
                     LQALLELKNERRERLSVYATEQGLKYFDGQKPWGVKCDIALPCATQNELDTEDAKELV
                     KNGCICVAEGANMPTTLGGVEVFIGAKILYAPGKAANAGGVATSGLEMSQNAIRLSWS
                     REEVDQRLFNIMKAIHENCVENGTEADGFVNYVNGANIAGFKKVANAMLEQGIV"
     gene            complement(1969..2310)
                     /gene="bamE"
                     /locus_tag="PXB27_002381"
     CDS             complement(1969..2310)
                     /gene="bamE"
                     /locus_tag="PXB27_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HDL5347122.1"
                     /translation="MKMKSLLAAMLLALGVTACSTVEKVVYRIDVPQGNYLEQDKIAQ
                     LKVGMTAEQVEYLLGTPMLKNIFETHRWDYVFIKREGYKDPEQRNLFIYFDAKGLVSK
                     IDDSNANAEQK"
     gene            complement(2377..3378)
                     /gene="yejK"
                     /locus_tag="PXB27_002382"
     CDS             complement(2377..3378)
                     /gene="yejK"
                     /locus_tag="PXB27_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HDL5347123.1"
                     /translation="MSINVSEIVLHQLQKTEGENPELNTFLRENLLAISPEVDQMMLQ
                     LHQAYQSKAKAYGIFKPESVFAQQLNRLLEQETDFLPFSHSCAKMLAAELIKYPFADG
                     GTFILCRYTFLATEYLFIALIDSRNSMLVDEQLEIKRTQYLEIAQYDIACRINLTELK
                     LDATSNRYLTFVKGRVGRKIADFFMDFLSAEEGFNPQLQNQTLLQAVSDYCEQGELSA
                     PQTREVKKQVFEYCKGQINSGEEIALNELSNALPTLNEIDFAQFTQEQEYGLEENIPP
                     MRNALKTLTKFSGSGKGVTISFNAELLGERLIWDELNDTLTIKGLPANLRDQLERNK"
BASE COUNT          998 a          735 c          569 g         1086 t
ORIGIN      
        1 ataaatcatt ttgcgtacaa attaaatcaa gattttcata taaacaggca tatttcagct
       61 ctgtaatatt gggcaatata atagcaattt tattacttgt gccacttttt aacgtagagg
      121 cttgaatatt tttacgataa cccaactttt caacggcagc ttgtactttt tctattttag
      181 ctaagctaac attacttttt ccgtttaata cattcgatac tgttcctaac gaaacatttg
      241 cttcctttgc gatatctttc atcgtaatga tcatatactg ccctttcaca tactgctaaa
      301 actgagattg gcatagattt taccataatt ggcaaatgaa atacgatttc aatttagctt
      361 gtctctagct acaacctaaa ataaaaaaac tgcctcgata tatcgaggca gttcttgaat
      421 aatagggata ataaattaaa caataccctg ttctaacatc gcatttgcca cttttttgaa
      481 accagcaata ttcgcaccgt ttacatagtt tacgaaacca tctgcttcag taccattttc
      541 tacgcagttt tcgtgaattg ctttcataat gttgaataaa cgctgatcta cttcttcacg
      601 actccaagat agacgaattg cattttggct catttctaaa cctgaggttg ccacaccacc
      661 tgcgttagcc gctttacccg gagcataaag gattttcgca ccgataaaga cttcaacacc
      721 acctaatgtg gttggcatat ttgcaccttc tgccacacaa atacagccgt ttttcactaa
      781 ttctttagca tcttcggtat ctaattcgtt ttgggttgca caaggtaaag cgatgtcaca
      841 tttcacaccc cacggttttt gaccgtcaaa gtattttaag ccttgttctg ttgcatacac
      901 agataagcgt tcacggcgtt cgtttttcaa ctctaataat gcttgtaatt gtgcctctgt
      961 cataccggat tcagggaata agacataacc gttagaatct gaaaccgtta atactttacc
     1021 gcctttttga atcacttttt cagctgcata ttgtgccacg ttacccgaac cggaaattac
     1081 cactgatttt ccttcgatct gctgaccttt agtcgctaac atagactctg caaaatacac
     1141 cgcaccataa ccagttgctt ccggacgaat taagctacca ccccaagtca gcgatttacc
     1201 ggttaatact gaagtaaatt cattgcgtaa ttttttgtat tgaccgaaca tatagccgat
     1261 ttcacgacct cccacgccga tatcgcctgc aggaacgtca gtatcagcac caatgtggcg
     1321 atataattcg ctcataaacg cttggcagaa acgcattact tcggcatcag atttgccttt
     1381 cgggtcaaag tctgaaccac ctttaccacc gcccattggc aatgtcgtta acgcattttt
     1441 gaatacttgc tcaaaggcta agaattttaa tacgcctaaa tcaacggttg ggtggaaacg
     1501 aataccgcct ttgtatggac cgattgctga gttcatttgt acacggtaac cacggtttac
     1561 ttgcacttga cctttatcat caacccaagt gacacggaag ctaattacac gctcaggctc
     1621 aacaatacgc cctaataaac cttgctgggt atatttcgga tttttagcta agaaaggggc
     1681 aaggctaccg aacacttctt caaccgcttg atggaaaggg gcttggttcg gatcacgttg
     1741 tttgattttt tggaaaaggg cgtttaaatc gctcatatta tactcctgaa taaattgatg
     1801 atgttgtgta tttaagatga gcgaagtcta gcaagcaatt ttagctttga aaagatagat
     1861 tagaaaaatt tatcattaaa ataagaaaaa ttaagtttaa atgcttattt tttattcaaa
     1921 aaaataagcc cacttttaaa agtgggcttt tcgaaccaac aaatcgtcct atttttgttc
     1981 tgcattcgca ttgctgtcat caatttttga tactaaccct ttagcatcaa aataaataaa
     2041 cagattgcgt tgttcaggat ctttataacc ttcacgttta ataaacacat aatcccaacg
     2101 gtgggtttca aaaatgttct ttaacattgg tgtaccaagc aaatattcaa cttgttccgc
     2161 tgtcataccg actttaagtt gagcaatttt atcttgttct aagtaattgc cttgcggtac
     2221 atcaatacgg tacaccactt tctcaacggt tgaacaagcc gttacaccca gtgcaagcaa
     2281 cattgcagct aaaagagatt tcattttcat aattgacctt ttgataatcc aaaattcaga
     2341 taacgagata atacctaaac tgataaaaat tttccattat ttgttacgtt ccagctggtc
     2401 acgcagattt gccggtaatc ctttgattgt cagtgtgtcg tttagttcat cccaaattaa
     2461 acgctctcct aataattctg cgttaaagct aatcgttact cctttgcccg aaccggaaaa
     2521 tttcgtcagg gttttaaggg catttcgcat aggcggaata ttctcttcca agccgtattc
     2581 ttgctcttgc gtaaattggg caaaatcgat ttcattcagt gttggcaatg cgttggaaag
     2641 ctcatttaac gcaatttcct ctccactatt tatctgccct ttacaatatt caaacacctg
     2701 ctttttcact tctcgagttt gaggggcaga taactcccct tgctcacaat aatcgctcac
     2761 cgcttgcaac aaggtttgat tttgcaattg tggattaaac ccttcttccg cacttaaaaa
     2821 atccataaag aaatcggcaa ttttacgccc tactctgcct ttcacaaaag ttaaatagcg
     2881 atttgacgtt gcgtcaagtt tcagttcagt taaattaatg cggcaggcaa tatcatattg
     2941 tgcaatttcg agatattgcg tgcgtttaat ctctaactgc tcatcaacca gcattgaatt
     3001 acggctatca atcaacgcaa taaacaaata ttcggtagct aaaaaagtgt aacgacacaa
     3061 aataaaagtg ccaccatcgg caaacggata tttaatcagc tctgctgcaa gcattttggc
     3121 acagctatgg ctaaaaggta aaaaatcggt ttcttgctcc aacaagcggt tgagctgttg
     3181 agcgaaaacc gactccggtt taaatatacc gtaagccttt gctttacttt gataggcttg
     3241 atgtaattgc aacatcattt gatcgacttc aggggaaatt gccaataaat tttctcgcaa
     3301 aaacgtatta agctccgggt tctcgccctc tgttttttgt aattgatgta acacgatttc
     3361 agaaacatta atgctcatta attcactt
//
LOCUS       DANLUQ010000193         3386 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.194, whole genome shotgun sequence.
ACCESSION   DANLUQ010000193 DANLUQ010000000
VERSION     DANLUQ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.194"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..284)
                     /gene="ybgE"
                     /locus_tag="PXB27_002383"
     CDS             complement(<1..284)
                     /gene="ybgE"
                     /locus_tag="PXB27_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HDL5347124.1"
                     /translation="MIHKLYNLTAKGLLKALSFILAAVLFATIWVNSTAFALSFGGKT
                     PYLAMLVFYGMAILWVHGIGFEIRAAIWKVIFLPLLGYLIVIPALWILLV"
     gene            complement(284..382)
                     /locus_tag="PXB27_002384"
     CDS             complement(284..382)
                     /locus_tag="PXB27_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CydX/CbdX family cytochrome bd oxidase small
                     subunit"
                     /protein_id="HDL5347125.1"
                     /translation="MFYVTWVLGVLLAIFFATVITISIEKTGKFDE"
     gene            complement(397..1530)
                     /gene="cydB"
                     /locus_tag="PXB27_002385"
     CDS             complement(397..1530)
                     /gene="cydB"
                     /locus_tag="PXB27_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDL5347126.1"
                     /translation="MIDYEILRFIWWILIAVLLIGFAITDGFDMGVLNLLPIIGKSNV
                     ERRIMINTIAPHWDGNQVWLLTAGGAIFAAWPTVYATSFSGFYIAMVLVLAALFFRPV
                     GFEYRAKIDNPKWRSAWDWGLFIGGFVPSLVFGVAFGNLLQGVPFEFNNINQSQYTGS
                     FFGLLNPFALVCGIISLMMLTTQGATWLQMKTTDELHNNSRKVAQVTASIVLVAFILA
                     GVWLYFKDGFVITSELDHNAQSLLTNKTVAVQAGAWFRNYAEMPVLWVVPALVVVGAL
                     LTIIASKANRSGLAFFSSSLMIVGVILTAAVSMFPFIMPSITHPEMSLTVWDATASHT
                     TLTVMLFVAGIFVPLVLAYTIWSYIKMYGRLDASYLEENKAAY"
     gene            complement(1543..3093)
                     /locus_tag="PXB27_002386"
     CDS             complement(1543..3093)
                     /locus_tag="PXB27_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HDL5347127.1"
                     /translation="MLDVVELSRLQFALTALYHFLFVPLTLGLSFVLVVMETLYVTTG
                     KEVYKDMTKFWGKLFGINFALGVTTGITMEFQFGMNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWERLSKAKHLLATYCVAFGSNFSALWILVANGWMQNPIASEFN
                     FETMRMELSSFSELVLNPVTQSKFLHTVTAGYVCGAIFVLGISSYYILKGRDLGFAKR
                     SFSVGASFGLIAIISVLIMGDESGYEIGKAQPTKLAAMEGEFETQPAPAAWNAFVIPN
                     TAEMKNEFSIHIPYAAGIIATRSLDKEIKGLKDIRVINEQRVRTGMVAYGLLEKLRSG
                     NYTEADKEAFKAVQKDLGFGLLLKQYTPNVVDATEEQIQKAADNTIPNVGPTFWAFRV
                     MMLSGFLMLILITTAFIQNMRGKVESSRFLLRGLLFGLPLPWIAIESGWFLAEYGRQP
                     WAIYEMLPVGVSNSALTTGDLWFTIGLLCGLYTLFLVVEMYLMFKYGRLGPSALKTGR
                     YYFEQSTK"
BASE COUNT         1220 a          681 c          529 g          956 t
ORIGIN      
        1 ttaacaagta aaatccataa tgccggaata acaataaggt aacctagaag tggtaaaaaa
       61 atcactttcc aaatagcagc tcggatttca aatccaattc catgtaccca taaaatagcc
      121 ataccataaa ataccaacat tgctaaatac ggcgtttttc caccaaaaga aagagcgaaa
      181 gcggtcgaat ttacccaaat agttgcaaat aatacagccg ctaaaataaa agaaagggct
      241 tttaacaagc cctttgctgt taagttatag agtttatgaa tcattactcg tcaaacttac
      301 ctgttttttc aatgctgatc gtgatgactg ttgcaaagaa gattgcaagc aatacaccta
      361 agacccaagt tacataaaac atattgtgtc tccttattag taagctgctt tattttcttc
      421 taaataacta gcatctaaac gaccatacat tttgatatat gaccaaatag tatatgctaa
      481 tactaacggt acaaagatac cagcaacaaa taacattaca gttaatgttg tgtggctagc
      541 agtagcgtcc caaaccgtta agctcatttc tggatgagta attgacggca taatgaatgg
      601 gaacattgat actgcagcag ttaaaatcac cccaacaatc atcaatgatg atgagaagaa
      661 tgctaaacct gaacgatttg cttttgaggc aataatagtc agtaatgcac ctactacaac
      721 taatgcagga acaacccata atacaggcat ttcagcataa tttctaaacc atgcaccagc
      781 ttgaaccgct acagttttat tcgtcaatag agattgtgca ttatgatcta actcgcttgt
      841 gatcacaaaa ccatctttaa aatataacca tacacctgca agaataaatg ccactaatac
      901 aatagaagct gtcacttgtg ccacttttct tgagttgtta tgtagctcat cagtagtttt
      961 catttgtaac caagttgcac cctgtgtggt taacatcatt aagctgataa taccgcacac
     1021 taatgcaaat ggatttaata aaccaaagaa tgaacccgta tattgtgatt ggttaatatt
     1081 attaaattca aatggtacac cttgtaataa gttaccgaat gcaacaccga atactaatga
     1141 tggaacgaaa ccaccaataa ataatcccca atcccaagca ctacgccatt tcggattatc
     1201 aatttttgca cgatactcaa aacctacagg gcggaaaaat aatgctgcaa gcacaagcac
     1261 cattgcaata tagaaacccg agaacgaagt tgcatagact gttggccatg cagcgaagat
     1321 cgcaccacca gcggtcaata accatacttg gttaccatcc cagtgagggg caattgtgtt
     1381 gatcataata cgacgttcaa cattactttt accaatgata ggtaaaaggt ttaatacgcc
     1441 catatcaaaa ccatcagtaa ttgcaaagcc aattaacaat accgcaatta agatccacca
     1501 aataaagcgg agaatttcat aatcgatcat ttctaactcc tattatttag ttgattgttc
     1561 aaaataataa cggcctgttt ttaatgcact agggccaaga cgaccatatt tgaacattaa
     1621 atacatttct acaactaaga atagtgtata taaaccacac aataagccga ttgtaaacca
     1681 taaatcacct gttgttaatg cagagtttga cacaccgaca ggtaacattt catagatagc
     1741 ccaaggctga cggccatatt cggcaaggaa ccaaccagat tcaatcgcaa tccaaggtaa
     1801 cggaagaccg aataacaagc ctcttaataa gaaacggcta ctttctactt taccacgcat
     1861 attttgaata aatgctgtcg taatcaagat aagcatcaag aagccagata acatcattac
     1921 acggaatgcc cagaatgtag ggcctacatt tggaattgta ttatctgctg ctttttgaat
     1981 ttgttcttca gtcgcatcaa ccacattagg tgtatattgt tttaacaata acccaaagcc
     2041 taaatctttt tgtacagctt taaatgcttc tttatccgcc tctgtataat tgccagaacg
     2101 taatttttct aatagtccgt aggctaccat accggtacga acacgttgtt cattaattac
     2161 acgaatatct tttaggcctt taatttcttt atctaaagaa cgggttgcaa taataccagc
     2221 tgcataagga atatgaattg agaattcatt tttcatttcc gcagtatttg gaatcacaaa
     2281 agcattccaa gcagctggag caggctgagt ttcgaattca ccttccattg cagctaattt
     2341 agtcggctga gctttaccga tttcataacc agattcatca cccataatca gtacagagat
     2401 gatcgcaatt aaaccaaaac ttgcacctac tgagaatgaa cgttttgcaa agcctaagtc
     2461 acgacctttt aagatatagt atgaactaat acctaataca aaaatagcac cacaaacata
     2521 gcctgcggtt acagtatgta agaatttaga ttgtgtaact gggtttaata caagttcaga
     2581 gaaacttgaa agctccattc gcatggtttc aaagttgaat tcagaagcaa tcgggttttg
     2641 catccaacca ttagcaacta aaatccatag tgcagagaag ttagaaccaa acgcaacaca
     2701 gtaagttgct aataaatgtt tcgctttcga taaacgttcc cagccaaaga agaacaagcc
     2761 cacaaaggta gattctaaga agaacgccat taaaccttca atcgctaatg gagcgccaaa
     2821 aatatcacct acataatgag aataatatga ccagttcata ccaaactgga attccattgt
     2881 aataccagtt gttaccccta gggcaaagtt aataccaaat aatttacccc agaatttagt
     2941 catatcttta tatacttctt tacccgtagt tacatacagg gtttccatca ccacaaggac
     3001 gaaagaaaga cctaatgtaa gcggtacgaa taagaagtga tagagtgcag ttagagcaaa
     3061 ctgcaaacgt gagagttcaa ctacatctaa catctcaaac ctcttatatt aatatactaa
     3121 tttgacaatt agattactgt aaaagtaatc acccacaaat tcactcaatt ctaataaata
     3181 acccaaattg gttaatttta taataaagtg tcgacgagta ttctacctgt aaatagctaa
     3241 ttaaaaaata gtaattttgt tatattcgtc acaaaattgg tttatttatg taataaatgc
     3301 tatttaacat actaaataac acggttatac ttaacaaata agtaacaatt tatttactat
     3361 tttttatact aacgatcttg aaaatt
//
LOCUS       DANLUQ010000194         3303 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.196, whole genome shotgun sequence.
ACCESSION   DANLUQ010000194 DANLUQ010000000
VERSION     DANLUQ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.196"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(21..644)
                     /locus_tag="PXB27_002387"
     CDS             complement(21..644)
                     /locus_tag="PXB27_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL5347128.1"
                     /translation="MQERITQQNERLSAYMDGQNVDTAFVETLTNSPELQQKWASYHT
                     IRSVMQGDEIILGRDFSAKMEALLENEEIESQANVEKPKGLLLKLKRWGTPIMQAGIA
                     ASVCLVAVLGVNSMNASDEIAQTQQPVLQTLPFSNSVEAVSYNAPVQDQPTEEQLELQ
                     QRKINALLENHELQRRTNNVKNLTLSEEEKQKAQTSSNTESEPNIQK"
     gene            complement(690..1262)
                     /gene="rpoE"
                     /locus_tag="PXB27_002388"
     CDS             complement(690..1262)
                     /gene="rpoE"
                     /locus_tag="PXB27_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HDL5347129.1"
                     /translation="MSEQITDQELVEKAQKGDKKAFNLLVVRYQNRVAGLLTRYVARD
                     DIPDIVQESFIKAYRSLESFRGESAFYTWLYRIAVNTAKNHLTSLGRRPPKEDILAED
                     AESYDVGAQLRESDTPENLILSEELKKVVFDTIENLPDELKTAIRLRELEGLSYEEIA
                     EVMECPVGTVRSRIFRAREAIDAKIKPLMQ"
     gene            complement(1484..3028)
                     /gene="leuA"
                     /locus_tag="PXB27_002389"
     CDS             complement(1484..3028)
                     /gene="leuA"
                     /locus_tag="PXB27_002389"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDL5347130.1"
                     /translation="MSNNNIIIFDTTLRDGEQSLKASLTVKEKLQIALALERLGVDVM
                     EVGFPVSSAGDFESVQTIARHIKNSRVAALSRAVAKDIDAAYEALKVAEAFRIHTFIA
                     SSALHVEAKLKRSFDDVVEMAVAAVKRARNYTDDVEFSCEDAGRTGIDNICRIVEAAI
                     NAGATTVNIPDTVGFCLPNEYGNIIHQVMNRVPNIDKAIVSVHCHNDLGMATANSLTA
                     VQNGARQIECTINGIGERAGNTALEEVVMAIKTRQEFMGVDTRINTQEIHRVSQMVSQ
                     LCNMPIQPNKAIVGSNAFAHSSGIHQDGMLKNKNTYEIMSPETIGLKKEKLNLTARSG
                     RAAVKGHMADMGYTETDYDLDKLYEAFLQLADKKGQVFDYDLEALAFIDMQQGDEDRL
                     KLDVITTQLISGLPASAFVQVELDGVRQSATSQGGNGPVEATYNAIMNIVGMELKMSH
                     YNLTAKGEGAEALGQVDIVVEYEGRKFHGVGLATDIVESSARALIHAINAIYRSQKVA
                     DLKAKK"
BASE COUNT          894 a          724 c          585 g         1100 t
ORIGIN      
        1 tcagtaactt attttaccta ttatttttgt atatttggct ctgattctgt attggaagaa
       61 gtttgtgctt tttgcttttc ttcctcagat aaggttaaat ttttaacgtt attagtacgg
      121 cgttgtaact catggttttc taataacgca ttaatcttac gctgttgaag ctctaactgt
      181 tcttctgttg gttgatcttg aactggcgca ttataactta ccgcttcaac tgaattgcta
      241 aatggcaatg tttgtaatac tggttgttgc gtttgagcta tttcatcact cgcattcatt
      301 gaattaaccc ctaataccgc aactaagcac actgaagctg caataccggc ttgcataatt
      361 ggagttcccc aacgcttgag cttgagcaat aagcctttag gtttctctac atttgcttga
      421 gattcaattt cttcattttc cagtaatgct tccattttag cagagaaatc tctccctaaa
      481 atgatctcat caccttgcat cacactacga atagtgtgat aacttgccca tttttgctgt
      541 aattcagggc tgttagttaa tgtttctaca aatgctgtat caacattttg cccatccata
      601 taagctgaaa ggcgttcatt ttgttgagtg atgcgttctt gcataattga ctccaatgat
      661 ttaaatctct tctaaaataa tctttctatc tattgcataa gtggcttgat tttagcatca
      721 atcgcttccc ttgctcggaa aatacgggaa cgtacagtac caaccggaca ttccatcact
      781 tctgcgattt cttcgtaact tagcccttcc aattctctta aacgaatggc ggttttaagc
      841 tcatcaggta aattttcaat cgtatcaaac acgactttct ttaattcctc ggataatatc
      901 aaattctctg gcgtatcact ttcacgaagt tgcgcgccta catcataact ttcagcgtct
      961 tcggcaagaa tatcttcttt tggcggtctt ctgcctaaag aggttaaatg atttttcgca
     1021 gtattcactg caatgcgata aagccacgta taaaaagcac tttcgccacg gaaagactct
     1081 aaagaacgat aggctttaat aaaagattct tgcacaatgt ccggaatatc atctcttgca
     1141 acataacgag tcagcaaacc ggctactcta ttttgataac gtacaacaag taaattaaat
     1201 gcttttttat cacctttttg tgctttttcg actaattctt ggtcggtgat ctgttcgctc
     1261 aactgcggac tctcctaaat atcaatatgt cattttccat tgcacacttt gttagtgtgt
     1321 gctttatgac aaaagttcaa aaaatttgaa aaattgtgta attttttaca ttcctagata
     1381 aaacctactt aataaactaa gcaagcggtc ggattttagc gaaattttgc aaaaatttca
     1441 aaaaatccga ccgcttgtct atgttatgct ttcgctctat aagttatttc ttcgctttta
     1501 aatctgccac tttttgtgaa cggtaaatcg cattaatggc gtgaattaaa gctcgggctg
     1561 aagattccac aatatcggtt gccaaaccca caccgtggaa ttttcgacct tcatattcca
     1621 ctacaatatc cacttgccct aatgcttctg ctccttcgcc tttcgcagtc agattatagt
     1681 gcgacatttt tagctccatt cccacaatgt tcataatggc attgtaagtt gcctcaactg
     1741 gaccatttcc cccttgagat gtagcacttt gacgaacgcc gtctaattcc acctgtacaa
     1801 aagccgaagc cggtaaaccg ctgattagtt gggtggtaat tacatctaat tttaagcggt
     1861 cttcatcgcc ttgctgcata tcaatgaagg caagagcttc taagtcataa tcaaacactt
     1921 gccctttttt atcagcaagt tgcaagaagg cctcataaag tttatctaaa tcgtaatccg
     1981 tttccgtata acccatatca gccatatgcc ctttcaccgc tgctctacct gaacgggcag
     2041 ttaaattcag cttctctttt ttcaagccga tggtttccgg cgacataatt tcataagtat
     2101 ttttattttt cagcataccg tcttggtgaa tacctgaaga gtgagcaaag gcattgctac
     2161 cgacaatcgc tttgttcggc tgaatcggca tattgcaaag ctggctgacc atttggctga
     2221 cacggtgaat ttcttgggta ttaatgcggg tatcaacccc cataaattct tggcgggttt
     2281 taattgccat taccacttct tcaagagcgg tgttaccggc acgttcgcca ataccgttga
     2341 tagtacactc aatctgtctc gctccgtttt gcaccgctgt caatgagttg gcggtagcca
     2401 tacctaaatc gttatggcag tgaacagaga caatcgcttt atcgatattc ggaacacggt
     2461 tcatcacttg gtggataata ttgccgtatt cattcggtaa acagaaaccg actgtatccg
     2521 gaatattcac tgtggttgca ccggcattaa tggcagcttc tacaatgcgg cagatgttat
     2581 caattccagt acgcccagca tcttcacagg aaaactcaac atcatcagta taatttctgg
     2641 cgcgttttac cgctgcaacc gccatttcga ctacatcatc aaatgaacgt tttaatttag
     2701 cctcaacgtg taatgccgag ctggcaataa aggtatggat acggaatgct tctgctactt
     2761 ttaaggcttc ataagctgca tcaatgtctt ttgcaaccgc tcgggaaagc gccgccaccc
     2821 ggctgttttt gatatgacga gcgatggttt gtactgattc aaaatcgccc gctgaagaaa
     2881 ctggaaaacc gacttccatc acatcaaccc ctaaacgttc taaagcaagg gcaatttgca
     2941 atttttcttt tacggttaaa cttgctttta atgattgctc accatcacgt aaagtcgtat
     3001 caaaaataat aatgttattg ttgctcatag aagcctcttt ttaggtgttt ttatttgata
     3061 aaaaaagccc gcattttaag gtgcgggcaa gaatgtggat agtccattta ctttttccaa
     3121 tcaatcttta tcctcgcaca aaatacgata acagagataa tcggagtaag gtaaattttt
     3181 ccatgttgtg ttgtatgttt atgtttgcat tgttattcag tagttgaggt gtatattatc
     3241 cttatccaaa tcaatgtcaa tagcattttt ctcttcacgc aaccgtttac cagcccatta
     3301 att
//
LOCUS       DANLUQ010000195         3275 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.197, whole genome shotgun sequence.
ACCESSION   DANLUQ010000195 DANLUQ010000000
VERSION     DANLUQ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.197"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            157..1140
                     /gene="epmA"
                     /locus_tag="PXB27_002390"
     CDS             157..1140
                     /gene="epmA"
                     /locus_tag="PXB27_002390"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005605446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HDL5347131.1"
                     /translation="MNNLQLDNIDWQPTASIPHLIQRSKIITEIRKFFTDRGLLEVET
                     PILSEFSVTDVHLSTFNTQFQSPFSPEAKTLHLMTSPEYHMKRLLAAGSGPIFQLCKV
                     FRNEEAGKRHNPEFTMLEWYRPHFDMYRLINEVDDLLQQILDCEPAESFSYQFVFQTY
                     VGLDPLSATKAQLVEKARKHGLQCDDDEQRDTLLQFLFSEVVEANIGQERPTAVYHFP
                     STQAALAQISSEDHRVAERFEFYYKGLELCNGFHELDDADEQIHRFEQDNLQREKMGL
                     EPQQLDVRFLAALKAGFPNCSGVALGVDRLLMLAMDAEKIEEVISFGIEKA"
     gene            1222..2112
                     /gene="rarD"
                     /locus_tag="PXB27_002391"
     CDS             1222..2112
                     /gene="rarD"
                     /locus_tag="PXB27_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDL5347132.1"
                     /translation="MNISGKLQGWHYALLCYLTWGTFPIYWYPINQSGMPAEQILAQR
                     VLWSALFAVFLLLIFQQGRAVINAFKQPKVLGLFVLSSFMIGVNWLVYLWAIVNEHIL
                     DASLGYFINPIFNVFVGRLILKEQLNKAQLLALLFAISGILWLAIPAGQIPWVALILA
                     ASFCIYGLIRKLAPMEPLSGLALETLLMSPFAITYLCFCYHQNILVFSELNPLQMAVL
                     LCSGIATTLPLLWFAMGARRISMSLLGMLQYISPTLQFLCGVLIFNETLSAERAVGYI
                     LVWIGVGVFLLGMKKAGVKR"
     gene            complement(2123..2734)
                     /locus_tag="PXB27_002392"
     CDS             complement(2123..2734)
                     /locus_tag="PXB27_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL5347133.1"
                     /translation="MKYEAKPLIISPNHIQQRLHGKLLARALLIIALLMLAKFIYIAS
                     LSNYFTFYLMEKFQLNIAMAQLSLFTFLGAVALGAFVGGPIGDKIGRNAVIWFSFLGM
                     APFALLLPHADLFWTIVCAIFAGFIMSSAFSAMVVYAQEAVPGRVGLISGTMFGLMFG
                     IGGLSAAVLGFFADEYGLESIFKACAYLPLLGFAALGLPKRNI"
     gene            complement(2762..3275)
                     /locus_tag="PXB27_002393"
                     /pseudo
     CDS             complement(2762..3275)
                     /locus_tag="PXB27_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251153.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT          970 a          712 c          674 g          919 t
ORIGIN      
        1 gacagtgata gaaagaaaat ttctaatgtt taaaatgaga aaatttaatt tgttgcaaaa
       61 aagttaaaac cctctcttta tttttcttga tcggtaacta gggcaactca cttttttagg
      121 ctaaaatagc ctcacatttt caagcggtca ttttttatga acaatttaca actcgataat
      181 attgattggc aaccaacagc ctctattcct cacctgatcc aacgttcaaa aatcattacg
      241 gaaatccgca agttctttac cgatcgaggg cttttagagg tagaaacccc gattttaagc
      301 gaattttcgg tgacagacgt tcatctttcg actttcaata cgcagtttca atcgcctttt
      361 tcccctgagg cgaaaacact ccatctgatg accagcccgg aatatcatat gaagcgacta
      421 cttgctgctg ggagtggacc gattttccaa ctgtgtaaag tatttcgcaa cgaggaagcc
      481 ggtaagcgac acaaccctga atttacgatg ctggagtggt atcgcccgca ttttgatatg
      541 tatcgcttaa tcaatgaagt ggatgattta ctgcaacaaa ttttggactg cgaaccggca
      601 gaatcattca gctatcaatt tgtgttccaa acctatgtcg gattagatcc gctttctgcc
      661 accaaagcac aacttgtgga aaaagcccga aaacacggct tgcaatgtga tgatgatgaa
      721 cagcgtgaca ccttgttaca atttttattc agcgaggttg tcgaagccaa tatcgggcaa
      781 gaaagaccaa ctgccgttta tcatttccct tccacccaag cggcattagc tcaaattagc
      841 tcggaagatc accgtgttgc cgaacgtttt gagttttact acaaaggctt ggaactctgt
      901 aacggcttcc acgaattaga cgatgctgat gagcaaattc atcgcttcga gcaagataat
      961 ctgcaacgtg aaaaaatggg gctggagcca cagcaactag atgtgcgttt tcttgccgca
     1021 ctcaaagcgg gttttccaaa ctgctcaggt gtcgcccttg gggttgatag gctgttaatg
     1081 ctggcaatgg atgcggaaaa aattgaagag gttatttctt tcggcattga aaaagcctaa
     1141 ttttttatgc cgttttgtga tttgcaattt gcaaaaaaat cgacaaatat gaccgcttgt
     1201 acactcgttt aggaaagcaa aatgaatatt tcaggcaaat tacagggttg gcactatgcc
     1261 ctactgtgct acttaacttg gggaaccttt ccgatttatt ggtatccgat caaccaatcg
     1321 ggaatgcctg ccgagcaaat tttggctcaa cgggtgcttt ggtctgccct atttgctgtg
     1381 tttctgctgc tgattttcca acaagggcga gcggttatta atgcctttaa acagccaaag
     1441 gtactcgggc tgttcgtgct ttcttctttt atgattggcg tgaactggct ggtttactta
     1501 tgggcaatcg tcaatgagca tattttagat gccagtctag gttattttat caaccccatt
     1561 ttcaacgtgt ttgtcggtag gcttattcta aaagagcagc tgaataaagc ccaattattg
     1621 gctctgctgt ttgcaattag cggcatttta tggcttgcta ttcctgccgg acaaattccg
     1681 tgggtcgcat tgattctagc cgccagtttc tgtatttacg ggctgattcg taaacttgcc
     1741 ccaatggaac ctttgtctgg tttggcgtta gaaactttgc tgatgtcgcc ttttgccatc
     1801 acctatctct gcttttgtta ccaccaaaac attttggtat tcagtgaact caatccttta
     1861 caaatggcag tgctactctg ctccggcatt gccactacct taccattgct atggtttgca
     1921 atgggtgcaa gacgcatttc aatgtcgcta ctcggtatgc tgcaatatat ttcacctacc
     1981 ctgcaatttt tatgcggtgt gctgattttt aatgagacac tttccgccga acgtgctgta
     2041 ggctacattc tggtttggat tggggtgggg gttttcttat tgggtatgaa aaaagctggt
     2101 gtcaaacgct aaaaataaaa cgttaaatat ttcgttttgg tagccctaat gcagcaaaac
     2161 caagtagtgg caaataggca caggctttaa aaatactctc caagccatat tcatccgcaa
     2221 aaaaacccaa tacggcggca cttaagccgc caataccaaa cattaaaccg aacattgtgc
     2281 cggaaatcag ccctactctg cctggtacgg cttcttgagc ataaaccacc atagcggaga
     2341 acgctgatga cataataaaa ccggcaaaaa tcgcacacac aatcgtccaa aagagatctg
     2401 cgtgaggcaa caacaaagca aaaggtgcca tacctaagaa agagaaccaa atcaccgcat
     2461 ttcgtccaat cttatcgcca atcggcccgc cgacaaaagc gcctaaagca actgcaccta
     2521 aaaaggtaaa tagagagagc tgtgccattg ctatattgag ttggaatttt tccattaaat
     2581 aaaaggtgaa atagttactg aggctcgcaa tataaataaa tttcgctaac atcaataaag
     2641 cgataattaa taatgctctg gctaataatt taccgtgtaa gcgttgttga atatggtttg
     2701 gtgaaataat gagcggtttg gcttcatatt tcacccaacg gcttacttta tataaaaccc
     2761 atcataatgc caataataca aatgcaacaa cccaaatcac cgcactttgc cctaacggca
     2821 caataaataa tgcaactaag agcggaccta acgccgatcc actgtttcca cctacttgga
     2881 aaagcgactg agctgtacca aatttaccgc ccgatgccat tcgagccacc cgagaggctt
     2941 cagggtgaaa aatggcagag cctatcccca ttaacgcagc ggctatcagt aacacgccaa
     3001 aattcggcga aaaggcaagt aaaatcacgg aaagaagcgt aatcagcata ctgacgggca
     3061 ataaattcgg ttttgggtat cggtataaag cccaataccc ggctgaataa tagatgcggt
     3121 aatttgataa atgagtgaaa tcataccaat ttgagcaaaa ctcagttggt aattttcttt
     3181 tagcattgga tagatcgaaa ccattactgc ttgaactaaa tcattcaaca aatgagaaag
     3241 ggaaatcagc agtaaaatgg gcaaaaccat ttttt
//
LOCUS       DANLUQ010000196         3206 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.198, whole genome shotgun sequence.
ACCESSION   DANLUQ010000196 DANLUQ010000000
VERSION     DANLUQ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.198"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            6..842
                     /locus_tag="PXB27_002394"
     CDS             6..842
                     /locus_tag="PXB27_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012621753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDL5347134.1"
                     /translation="MLFACINTLIPKLTSISPIDSNVIALVQYLAAFIFLVPSMNNIG
                     FFSSLKTEHFGQHCFRIFLSAIGIQCWTMALANQLPIWQGIALLMTSPLFVTIGSGLF
                     LKEKVDKKRWIATAMGFVGAMIILEPWSDHFEMIALLPIAAAFFWAGYSLMVKKLSRY
                     DSPTTMVAYLFILITPFNLLIALTNLSPSGFSLPSWSDFGFLILLGFFTALAQLAVAK
                     AYSVADASYIQPFDFIKLPLNVLAGWLVFNWVPPGKLWLGAAIIIAATIYITHAETKQ
                     SN"
     gene            complement(921..1412)
                     /gene="ilvN"
                     /locus_tag="PXB27_002395"
     CDS             complement(921..1412)
                     /gene="ilvN"
                     /locus_tag="PXB27_002395"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HDL5347135.1"
                     /translation="MRRTLAVLLENESGALSRVVALFSQRGFNIESLTVAPTDDPSLS
                     RMTIVAQGEEQVLEQIEKQLHKLIDVFKVSNLSAYEHVEREVLLVKVRATGSSRDELK
                     RMVDIYRGQIVDLTPKLYTIQLSGSTEKLNAFIEAVKEETTIVEIVRSGVISLSRGDK
                     NAL"
     gene            complement(1405..3126)
                     /locus_tag="PXB27_002396"
     CDS             complement(1405..3126)
                     /locus_tag="PXB27_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HDL5347136.1"
                     /translation="MKKLSGAEMIVQSLKDEGVEYVFGYPGGSVLDIYDAIHTLGLNH
                     VLVRHEQGAVHMADGYARSTGKVGCVLVTSGPGATNTITGILTAYTDSVPLVILSGQV
                     PSSLIGTDAFQESDMIGISRPVVKHSFLVKNTEEIPEIIKKAFYIASTGRPGPVVIDI
                     PKDVVNPLNKYPYEYPQEVSMRSYNPTVQGHKGQIKKALKALLVAKRPVLYVGGGVIS
                     AEGSPEITEFAQKLNLPVTCSLMGLGAYPASDKQYLGMLGMHGTFEANNAMHESDLIL
                     GIGVRFDDRTTNNLAKYCPNAKVIHVDIDPASISKTVQAYIPIVGSAKDVMEEFLSLL
                     GEENLAKNQVDLTAWWQQIDAWKARNCLAFEENSDVIKPQQVIRMIHKLTNGEAYVAS
                     DVGQHQMFAALHYGFDKPRRWINSGGAGTMGFGLPAAMGVKFAHPDATVVCVTGDGSI
                     QMNIQELSTAKQYDTPIVIVSLNNRFLGMVKQWQDIIYSGRHSQVYMNSLPNFAKLAE
                     AYGHVGIQIDHPSELEAKLTEAFSIKDKLVFVDVLVDETEHVYPMQVRGGAMNEMMLS
                     KTERTDA"
BASE COUNT          883 a          695 c          601 g         1027 t
ORIGIN      
        1 ccggtgtgtt atttgcctgt atcaatacct taattcccaa attaacgtcc atttcaccga
       61 ttgactcgaa cgtgattgca ttggtgcaat atttagctgc ctttattttc ctcgttccca
      121 gcatgaataa tatcggtttc ttctcttcat taaaaaccga gcattttggg caacactgtt
      181 tccgtatttt tctatctgcg attggtattc aatgttggac aatggcactc gctaaccaat
      241 tacctatttg gcagggaatt gcgttattga tgacttctcc attgtttgtg accatcggct
      301 ccggtctgtt tttgaaagag aaagtggata aaaagcgttg gattgcaacc gcaatggggt
      361 ttgttggggc gatgattatt ctagagcctt ggagcgatca ttttgaaatg attgcgttac
      421 tgccgattgc cgcggctttt ttctgggcag gttattcgct aatggtgaaa aaactctctc
      481 gttacgatag cccaactacg atggtcgctt acctgtttat tctaattacg ccttttaatt
      541 tgttgattgc actcacgaac cttagcccta gtgggtttag tctgccttct tggagcgatt
      601 tcggcttttt aatcttactt ggtttcttta cggcgttagc gcaactggcg gtagccaaag
      661 cctacagtgt agcagatgct tcctacattc agccgtttga ttttattaaa ttacctttga
      721 acgtattagc cggttggctt gtatttaact gggtgccacc gggtaaatta tggctaggag
      781 ctgcgattat tattgctgcg accatttata ttactcacgc tgaaactaaa cagagtaatt
      841 agcaacaaat tcacgctaca taaaaatccc tttatctcaa agataaaggg atttgcaaat
      901 tttaagctaa aattgaccgc ttacagagcg tttttatcgc cacgagatag actaatcacg
      961 ccagagcgaa caatctctac aatcgttgtt tcttctttaa ctgcctcaat aaatgcattg
     1021 agcttctcgg tagaaccgga taattgaatg gtgtaaagtt tcggtgttaa atctacgatt
     1081 tgcccacggt aaatatctac catacgtttc agttcatcac gagatgaacc tgtagctcgc
     1141 acttttacca ataacacttc acgctcaacg tgttcataag cacttaagtt actcacttta
     1201 aacacatcaa tgagtttgtg tagttgcttt tcgatttgtt ctaacacctg ttcttcgcct
     1261 tgcgcgacaa ttgtcatacg ggaaaggctt ggatcatcag taggggcaac ggtcaagctt
     1321 tcaatgttaa aaccacgctg cgagaaaagg gcaactacac gagataatgc acctgattca
     1381 ttttctaata atacggctaa tgttctacgc atctgttctc tccgttttac ttaacatcat
     1441 ttcattcatt gctcctccac gcacttgcat tgggtaaaca tgttccgttt catcgaccaa
     1501 tacatcaaca aatacaagct tatctttaat tgagaatgcc tcagttaatt ttgcctccaa
     1561 ttcagaaggg tggtcgattt gaatcccaac gtggccataa gcctctgcta atttagcaaa
     1621 gttaggtaga gaattcatat acacctgtga atggcggcca gagtaaataa tatcttgcca
     1681 ctgtttcacc atacctaaga aacggttatt taagctgaca atcacaatcg gtgtgtcgta
     1741 ttgctttgcg gtggaaagtt cctggatatt catttggata ctaccatcac cggttacaca
     1801 aacaacagtg gcatcagggt gggcaaattt tacccccatc gcagcaggta agccaaagcc
     1861 cattgtgcct gcaccgcctg aattaatcca acgacgtggt ttatcgaacc cataatgcaa
     1921 tgcagcaaac atttgatgtt gtccaacatc tgaggcaaca taagcctccc cattggtcaa
     1981 cttgtggatc attctaatca cttgttgtgg cttaatcaca tcactgtttt cttcaaaggc
     2041 taagcaatta cgagctttcc aagcatcaat ttgttgccac caagcggtta aatctacctg
     2101 attttttgca agattttcct cacctaataa agataaaaat tcttccatta catccttagc
     2161 actgcctaca attggaatat aagcttgtac tgttttggaa attgaggctg gatcaatatc
     2221 cacgtggatc acttttgcat tcgggcaata ttttgctaag ttatttgttg tacgatcatc
     2281 aaaacgtacc ccaataccta aaatcaaatc actttcgtgc attgcattat ttgcctcaaa
     2341 agtgccgtgc atgcctaaca tacctaaata ttgcttatca gaggcaggat aagcacctaa
     2401 tcccattaat gaacaggtta caggtagatt tagcttttgt gcaaactctg taatttctgg
     2461 cgaaccctct gcactaatta caccaccgcc tacataaagc actggacgtt ttgctactaa
     2521 tagtgctttt aatgcttttt taatttgccc tttatgccct tgaactgttg ggttataaga
     2581 acgcattgat acttcttgcg gatattcata gggatattta tttaatggat tgactacatc
     2641 tttaggaata tcaataacta ccgggcctgg tcgaccggtt gatgcaatat aaaaggcttt
     2701 tttgataatt tcaggaattt cttctgtatt ttttactaag aaactgtgtt ttacaaccgg
     2761 acgagaaatg ccgatcatat cactttcttg gaatgcatct gtcccaatta aacttgatgg
     2821 aacttggccc gacagtatta ctaacggaac agagtcggta taagcagtta aaataccagt
     2881 aattgtattc gttgctcccg gacctgaagt cactagcaca cagccgactt tacctgttga
     2941 acgggcatag ccatcagcca tatgtacagc accttgctcg tgtcgaacta aaacgtgatt
     3001 caatccaagt gtatgaattg catcataaat atcgagtaca gagccacccg gatagccgaa
     3061 aacgtattct acgccttcat cctttaagga ctgtacaatc atttctgcac cagaaagttt
     3121 tttcattgaa tcctccaaca aaatagcatt ttcttctcta agatggttgg attttgcagt
     3181 aaaattttat tgatgtctag ttctta
//
LOCUS       DANLUQ010000197         3176 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.199, whole genome shotgun sequence.
ACCESSION   DANLUQ010000197 DANLUQ010000000
VERSION     DANLUQ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.199"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..561)
                     /gene="pdxT"
                     /locus_tag="PXB27_002397"
     CDS             complement(<1..561)
                     /gene="pdxT"
                     /locus_tag="PXB27_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HDL5347137.1"
                     /translation="MKIAILALQGAFSEHTDKLQQLGVESMEIRQLADLNQDFDGLIL
                     PGGESTVQGKLLRELGLLEPLRQKILNGLPTLGTCAGLILLAENLVNDDKKHLATLPV
                     TVKRNAYGRQLGSFLVEEEVKYLGKIPLTFIRAPYIESTGKDVEILAKISGKIVGVKY
                     QNQIGISFHPEVNNDLSFHRYFVEMCR"
     gene            complement(561..1433)
                     /gene="pdxS"
                     /locus_tag="PXB27_002398"
     CDS             complement(561..1433)
                     /gene="pdxS"
                     /locus_tag="PXB27_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HDL5347138.1"
                     /translation="MTNRYDLNRQLAQMLKGGVIMDVTTPEQACIAEEAGACAVMALE
                     RIPADIRAAGGVSRMSDPKMIKSIQAAVSIPVMAKVRIGHVTEAQILQAIEIDYIDES
                     EVLSPADDTFHINKRKFEVPFVCGAKNLGEALRRINEGAAMIRSKGEPGTGNVVQAVH
                     HLRKMKQEIARLVSHTTDELYHVAKELQVPFDLVQYVHQYQKLPVVTFAAGGVATPAD
                     AALMMQLGAEGVFVGSGIFKSGDPAKRARAIVQAVTNYNDPKLLAELSEDLGEAMVGI
                     NEQEIELLMAARGV"
     gene            1644..3047
                     /locus_tag="PXB27_002399"
     CDS             1644..3047
                     /locus_tag="PXB27_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDL5347139.1"
                     /translation="MLTYTFNTQEGTALYEQLYRFLKNDIESNILIAGDKLPSKRAFA
                     KHLGISVMTVETAYQQLVAEGYVNTQPKKGFFVNALNLPEKKEAISVQLQKISSEQYE
                     KNKKWQADLTNRQTLAENFPFSVWTKLVREVLKTHQSELMERAESGGVWTLKFAIANH
                     LRAFRGMIVNPTQIIIGAGTEYLYGLLIQLLGLDKHYALTEPSYDKLRRIYHSYRLST
                     ATVSMPFSIDELNSKNVDVIHTSPSHHFPTGHVMPIAKRYELLAWVAQKAERYIVEDD
                     YDSEFRFVGQPIPALKSIDMLGRVIYMNTFSKTLSSTVRIAYMVLPPELLNRFQQELG
                     FYASTVSNFEQYILAEFIQQGYFEKHINRMRAYYQKKRDKLLKAFKQGNLAEKIAIKE
                     EHSGLHFIVQLNTTLTERQILQAAEEQGIKFVALSAYYQDKSQIQQNAFIIGYSNLKD
                     DQINYVAESLKKILDNE"
BASE COUNT          989 a          634 c          584 g          969 t
ORIGIN      
        1 tcggcacatt tccacaaaat agcggtgaaa acttaaatca ttattcactt cgggatggaa
       61 ggatatacct atctgatttt ggtattttac tccaacaatc ttaccgctta tcttggctaa
      121 aatttctaca tcttttccag tgctttctat ataaggcgca cgaatgaatg tgagaggaat
      181 tttaccgaga tatttaactt cttcttctac taaaaacgag cctaattgcc ttccgtaagc
      241 attacgtttc actgttacag gcaaagtagc caaatgtttt ttatcgtcgt ttaccaaatt
      301 ttccgcaagc aaaattaaac ccgcacaagt tccaagcgtt ggcaagccat ttaaaatttt
      361 ttgacgaagc ggctcaagca agcctaattc acgtaataat ttaccttgaa cagtcgattc
      421 gccaccaggt aaaatcagac catcaaaatc ttggtttaaa tcagcaagtt gacgaatttc
      481 catactttca acccctagtt gctggagttt atcagtatgc tcgctaaatg caccttgcag
      541 agcaagaata gcaattttca ttagacacct cttgctgcca ttagcaattc aatctcttgc
      601 tcattaattc ctaccattgc ttcacctaaa tcttcagaaa gctccgcaag taatttagga
      661 tcgttgtagt tggtaacagc ttgtacaatg gcccgcgctc gtttagcagg atcgcctgac
      721 ttgaaaatac ctgagccaac gaagactcct tctgcaccta actgcatcat caatgcagca
      781 tcggcagggg ttgcaacacc tccagcagca aaggtgacaa caggcaattt ttggtactga
      841 tgtacatatt gtactaaatc aaatggtact tggagctctt tagctacgtg ataaagttca
      901 tctgtggtat gtgaaaccaa gcgggcaatt tcttgtttca ttttgcgaag atgatgtact
      961 gcttgcacca cattgcctgt tcctggctcg cccttactac ggatcatagc agcgccttca
     1021 ttaatacgac gtagggcttc gcctaaattt tttgctccac atacaaatgg cacttcaaat
     1081 ttacgcttgt taatgtggaa ggtatcatct gcaggagaaa gcacctcact ttcgtcaata
     1141 tagtcaattt cgatcgcttg caggatttgt gcttctgtaa cgtgtccaat acgtactttc
     1201 gccattacag gaatggatac cgctgcttgg atactcttta tcatcttggg atcactcata
     1261 cgagaaacgc caccagctgc acgaatatct gcaggaattc tctctagtgc cattactgca
     1321 caagctccag cttcttccgc aatgcaggct tgctcagggg tagttacatc cataattaca
     1381 ccgcctttta acatttgagc aagttgtctg tttaaatcat agcgattagt cattgtactt
     1441 tttccttcta attgttgaat aattgaactt ttcgtagttt cgattagctc ggcattaagt
     1501 ttatttttat attcgctgga tgcgagtgtt catctaccaa tttaaataca ctcagccaat
     1561 ttgagtgagg aaacagcatg tattgtaatt taatctgaac tgtttaaaat gaccatttta
     1621 tgtttttctt atcatgtcag attatgctta cctacacttt taacacacaa gaaggcaccg
     1681 ctctctatga gcaactttac cgttttctaa aaaacgacat tgaaagcaac atacttatag
     1741 caggggataa actgccgtct aaacgggctt ttgcaaagca tttaggcatt tcagtaatga
     1801 cagtggaaac agcttaccaa cagttagtag cagaaggtta tgtgaatact cagcctaaaa
     1861 aaggcttttt tgtgaatgct ttaaatttac cagaaaaaaa ggaagccata tcagttcagc
     1921 tacaaaaaat cagttcagaa cagtatgaga aaaataaaaa atggcaggca gatttaacta
     1981 atcgacagac tttagcagaa aattttccgt tttcggtttg gacaaaatta gtacgcgaag
     2041 tgctgaaaac acaccaaagt gagctaatgg agcgtgctga gagtgggggc gtatggacat
     2101 taaaatttgc gatagccaat catcttcgag cctttagagg aatgatcgtt aatccaacac
     2161 agattattat tggagcgggt acggagtatc tttacggttt gctcattcag cttctaggct
     2221 tagataaaca ttatgcacta actgagccca gttacgataa attgcgcaga atttatcaca
     2281 gttatcgctt atcaacagcc acagtatcga tgccattttc aatcgatgag ttaaattcaa
     2341 agaatgtgga cgtgatacat acctcgccat cacaccattt tccgacagga cacgtaatgc
     2401 cgattgccaa acgttatgag ttgttagctt gggttgcaca aaaagctgaa agatatattg
     2461 tagaagacga ctacgacagc gaatttcgtt ttgtaggaca accaattcct gcattgaaat
     2521 ctattgatat gctcgggcga gttatctata tgaatacttt ttctaaaacg ctctcttcaa
     2581 ctgtacgaat tgcctatatg gttttgccac cagaattact gaatcgtttt cagcaggaat
     2641 tagggtttta tgcttctacc gtttccaatt ttgagcaata tattttggca gaatttattc
     2701 aacaaggcta ttttgaaaag catatcaatc gaatgcgagc gtattatcaa aaaaagaggg
     2761 ataaattact aaaagctttc aagcaaggca atttagcaga aaagattgca ataaaagaag
     2821 aacattcagg cttgcatttc attgtacagc taaacacaac attaacagaa aggcaaattt
     2881 tgcaggcagc agaagaacaa ggtataaaat ttgtcgcatt gtctgcttat tatcaagata
     2941 aatctcaaat tcagcaaaat gcttttataa ttgggtactc taatttaaaa gatgatcaga
     3001 ttaattatgt agcagagagc ctgaaaaaaa ttttagacaa cgaatagcaa gtatcaaaac
     3061 tgctttaatt tttagcaaat tgccttaaaa acacgcaagt ggtaattatt ttagtgattt
     3121 ttgcaaaaaa ttaccaaatt ctccttgcaa ggcattttgt tttgcgtata atgcat
//
LOCUS       DANLUQ010000198         2958 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.200, whole genome shotgun sequence.
ACCESSION   DANLUQ010000198 DANLUQ010000000
VERSION     DANLUQ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.200"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..241
                     /locus_tag="PXB27_002400"
     CDS             <1..241
                     /locus_tag="PXB27_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDL5347140.1"
                     /translation="QKSIDEQLKNGDYYIIKSLDFRPRRVFERSAEKKKIHNLISRAH
                     YDMATNEDSASEHLELFGEDIAEDSFDYPKPEKLI"
     gene            248..319
                     /locus_tag="PXB27_002401"
                     /pseudo
     CDS             248..319
                     /locus_tag="PXB27_002401"
                     /inference="COORDINATES: protein motif:HMM:NF013704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            329..847
                     /locus_tag="PXB27_002402"
     CDS             329..847
                     /locus_tag="PXB27_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HDL5347141.1"
                     /translation="MNRRYIGIEIGEHAKTHVVPRLKKVIEGEQGGISKAVNWQGGGS
                     FRFCELGEEVFDAFGSLNPNIRFDDLAAHIWYLENHFPLQKNSEKSPLVGTFNGKAYY
                     LLYNGILGDKRPQSGNVLTRKLMAELPYFAEFIQQGMDIVVYGEACRLGEVQLAEKKI
                     TFKQIPYDVMSR"
     gene            863..1852
                     /locus_tag="PXB27_002403"
     CDS             863..1852
                     /locus_tag="PXB27_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL5347142.1"
                     /translation="MKLKNYQNEALNRVSHYFSACLQSDAKTAFSQIQPEYSYKIPSE
                     HSNLRDVPYVCVRIPTGGGKTLLASHSIARIAKQYLDCDFPVTLWPVPSRTIKTQTTE
                     ALKNPQHPYRAVLDKTFNREVMVIESEDFDLLRPQDFGNKAIVIVSTIQNFRVENQDG
                     RKIYAFNEKLTAHFERLLPHIQANLEKISTTDLQENGLTSKQTGKIKCSFANLLRAYQ
                     PLVIVDEAHNARTSLSFDVLSQLSPSAILEFTATPNTDRKAGSNVLYHVSASHLKAEE
                     MIKLPIVLTEHHNWQQAIDEAVINRGALAQKAQYESEYVRPIYCFKLSRKTAK"
     gene            1819..>2958
                     /locus_tag="PXB27_002404"
     CDS             1819..>2958
                     /locus_tag="PXB27_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347143.1"
                     /translation="MFQAEPKNGKVTVEVLKQYLIDELKIDEEEIAIVIGNQHELDNI
                     DLFNPQCKINYIITVEALKEGWDCPFAYIFCSVQNVSSSKEAEQLLGRVLRMPYAKHR
                     QIEDLNRAYAPLSSKSFGETARNLQDKLIGMGFEALEVAEMLRIPQQENWIGNEGDLF
                     YTPTTVLALTQLPEMSDLSEAKRSQLKITEQEGHILVQVKGNLSEKLEKALIRTATGK
                     KKEQIEQQLQIHQNRVEILSSPAEKGEIFKVIPQLCMNIQGEFDLADSQVLLDYQSWN
                     LLDYPAKLDGFRLQENSQSFEIDVEDKRVSYHFNQQQLSFSDDWLRNPVKRGFSLPLA
                     HQNFYPDFIVELIDGRILIVEYKGEQLKSNEDSKEKALIGNLWEKL"
BASE COUNT         1005 a          491 c          597 g          865 t
ORIGIN      
        1 ccaaaaaagt attgatgagc aattaaaaaa tggtgattac tatattataa aaagtttaga
       61 ctttcgacca aggagagtat ttgagcgaag tgcggaaaag aaaaaaattc ataacttaat
      121 tagcagagct cattatgata tggcaacaaa tgaagactct gctagtgaac atctagaatt
      181 atttggggag gatattgcag aagattcatt tgattatcct aaacctgaaa aattaatcta
      241 atttgtatta gaagcatcga ctgaaccata tgatctcgtc ctcgatagct ttcttggctc
      301 aggtacaact gcagcagttg ctcataaaat gaaccgtcgt tatatcggta ttgagattgg
      361 cgaacatgct aaaacccatg ttgtcccacg gttgaaaaaa gtaattgaag gtgagcaagg
      421 cggtatttca aaagcagtaa attggcaagg tggtggatcg tttcgctttt gtgagttagg
      481 cgaggaagta tttgacgcat tcggttcact taatccgaac attcgttttg acgatttagc
      541 cgcccatatt tggtatttag aaaatcattt ccctttgcaa aaaaacagcg aaaaatcacc
      601 gcttgtcggc actttcaacg gcaaagccta ctatttgtta tataacggaa ttttaggcga
      661 taaacgtccg cagagtggta atgttctgac acgaaaatta atggcggagt taccatactt
      721 tgctgaattt attcaacagg gaatggatat tgtggtatat ggcgaagcgt gccgtttagg
      781 agaagtgcag ttagctgaaa agaaaattac atttaaacag attccatatg atgtaatgtc
      841 tcgttaatag taggagaaaa aaatgaaatt aaaaaattac caaaatgagg cattaaaccg
      901 tgtcagccac tatttttcgg cttgtttgca atctgatgca aaaaccgcat ttagccaaat
      961 tcaacctgaa tatagttaca agatcccaag tgaacacagt aatttgcgtg atgtgcctta
     1021 tgtttgtgta cgaattccaa ctggtggcgg taaaacgttg ttagcctcgc atagtattgc
     1081 tcgtattgca aagcagtatt tggattgcga tttcccggtt accttgtggc cagtgccttc
     1141 tcgtacaatt aaaacccaaa ctacagaagc attaaaaaat ccgcaacacc cttaccgtgc
     1201 agtacttgat aaaactttta accgagaagt aatggttatt gagtctgaag attttgattt
     1261 acttcgccca caagattttg gtaataaggc gattgtaatc gtttctacta ttcaaaattt
     1321 tcgggtagaa aatcaagatg ggcgaaaaat ctatgctttt aatgaaaaat taacagctca
     1381 ttttgagcgt ttactacccc atattcaggc aaatttagaa aagatttcca ctactgatct
     1441 acaagagaac ggtttaacct ccaaacaaac gggcaaaatt aagtgttctt ttgcaaattt
     1501 gttgcgagct tatcagcctt tggtcattgt tgatgaagca cataatgctc ggacttcact
     1561 ctcttttgat gtgctatctc aactttcccc atcagctatt ttagaattta ctgctacgcc
     1621 aaacacagat agaaaagcag gaagtaatgt gttgtatcac gtttcggcaa gccatctaaa
     1681 agcagaagag atgatcaaat tgcctattgt gctgaccgaa catcacaact ggcaacaagc
     1741 aatagatgag gctgtaatca atagaggagc attagcacaa aaagcacagt atgagagcga
     1801 gtacgttcgt ccgatttatt gtttcaagct gagccgaaaa acggcaaagt aacagtagaa
     1861 gtcttaaaac agtatttaat tgatgagtta aaaattgatg aagaagagat tgccattgtg
     1921 ataggtaatc aacacgaatt agataatatc gatttattta atccacaatg caagatcaat
     1981 tacatcatca cggttgaggc attaaaagaa gggtgggatt gcccttttgc ttatattttt
     2041 tgctcagtac aaaatgtttc ctctagcaag gaagcggagc agctattagg gcgagtattg
     2101 cgaatgcctt atgctaaaca ccgtcaaatt gaagatttaa atcgtgctta tgctccgctt
     2161 tcctctaagt cgttcggcga aacggctcgt aatttacaag ataaattgat tggaatgggg
     2221 tttgaagcat tagaagtagc tgaaatgctt cgtatcccac aacaagaaaa ttggattgga
     2281 aacgaaggag atttattcta tacaccgaca acagttttag cattaacgca actccctgaa
     2341 atgtctgatt taagcgaagc aaaacgttct caacttaaaa ttaccgagca agaaggtcac
     2401 attttagtgc aagtaaaagg taatttaagt gaaaagttag aaaaagcgtt gattcgaact
     2461 gcaacaggta agaaaaaaga gcaaattgag caacagttac aaattcacca gaatagagtt
     2521 gaaatattat cttctcctgc cgaaaaaggg gaaatattta aagtaatacc tcaactgtgt
     2581 atgaatattc aaggcgaatt cgacttagct gattcacaag tgctattaga ttaccaaagt
     2641 tggaatttat tggattatcc agccaaactt gatggattta gattacaaga aaatagccaa
     2701 agttttgaaa ttgatgtgga agataaacgg gtttcttacc attttaatca gcagcaactg
     2761 agtttttctg atgattggtt aagaaatcct gtaaaaagag ggttttcttt accattagct
     2821 catcagaatt tttaccctga ttttattgta gagcttattg atggtcgaat tttaattgtg
     2881 gaatataaag gggaacaatt aaaaagtaat gaagattcta aagaaaaagc cttaattggc
     2941 aatctttggg agaaatta
//
LOCUS       DANLUQ010000199         2944 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.201, whole genome shotgun sequence.
ACCESSION   DANLUQ010000199 DANLUQ010000000
VERSION     DANLUQ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.201"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..544
                     /locus_tag="PXB27_002405"
     CDS             <1..544
                     /locus_tag="PXB27_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347144.1"
                     /translation="SSLEFDESKQEKINSFLIVGNKLYVVGELHDYEFEHQQVAELNN
                     FLKSLIANKIKSVEANFEVKESKKVEGSYRIILDGKSLTESERSTVMDTFHFNSAQNP
                     SITYQAEGNLVKLANREELLQKFRFKQALEANVRYYKMKSGLQNTAEGALSIVMLPVA
                     VVAVLPFYMFWVGACATYGC"
     gene            555..1040
                     /locus_tag="PXB27_002406"
     CDS             555..1040
                     /locus_tag="PXB27_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HDL5347145.1"
                     /translation="MYSLYLKILSECDWCCDPKTLSTYQQEWLLHEGSLTQKLYDVAT
                     NFNVEVVQEKWIAKNSEKTTACNEDVWSREVLLKDGEKNWIFAQTLVPRSTIENIAQN
                     VLELGNQPIGLWLFSQNPTRTKLEWTQTENGLFARRGVYEIRGYSLEIRELFLETFPY
                     L"
     gene            complement(1078..1947)
                     /locus_tag="PXB27_002407"
     CDS             complement(1078..1947)
                     /locus_tag="PXB27_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDL5347146.1"
                     /translation="MKNINTQAVGWIGLGQMGEPMAKHLLNSGVNVGVYNRDPAKAEK
                     VVEAGGTLYPSVLELVKAYDVIFLMIADYAAVQQVLSDEVLAELNGKVVVNMSTISPS
                     QNQAVEALLAQHGAEFVEAPVSGSSKVAEAGKLLVLAAGKEETVELLKPLFAAFSTTT
                     FYYGEVGKAGGIKLMINSLLGIFIQAYGEALNFADQYGIPKEKVIEMISGSFMNSPIF
                     QAKVPMYQANEFPPAFMMKHMTKDFNLASEEIAKLGKSYPLIEQATKTYNQANDSGLS
                     EVDMAAIYQFLAK"
     gene            2077..2868
                     /locus_tag="PXB27_002408"
     CDS             2077..2868
                     /locus_tag="PXB27_002408"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDL5347147.1"
                     /translation="MKPTILLYDSGMGGLTIYDEIRKVLPHAHYLYCFDNAFFPYSEK
                     SEAVLIERAVKIVQKIAEKQPLDMVVVACNTASTVVLPALREHFNFPIVGTVPAIKPA
                     AAISETKVIGLLATKGTVARPYVDELIRKYAQDCTVEKIGTTDLVEIVEEKQRSGKVD
                     MNRLQKVVEAWQGKPELDTVILGCTHFPLVKAELQQLLPDVKYFVDPGVGIAKRVLSL
                     LEERKFQQKADFENQAFCTKQSSAFNIKQDVMQNWGFNNLSVIDI"
BASE COUNT          900 a          550 c          612 g          882 t
ORIGIN      
        1 ttccagttta gaatttgatg aaagtaaaca agaaaaaatc aattcattct tgattgttgg
       61 taataaatta tatgtggtgg gtgaactgca cgattatgaa tttgagcatc agcaagttgc
      121 ggaactgaat aattttctca aaagcctaat tgctaataaa atcaaatctg ttgaggcaaa
      181 cttcgaggtt aaggaatcca aaaaagtgga aggtagttat agaattattt tagatgggaa
      241 atcacttaca gaaagtgaaa gaagtacggt gatggatacc tttcatttta attcagctca
      301 aaatccatct attacttatc aggcggaagg gaacttggtg aaattagcca atagagaaga
      361 gcttttgcaa aaattccgat ttaaacaagc gcttgaggcg aatgtgcgtt attacaaaat
      421 gaaaagtggt cttcaaaata cggctgaagg agcgttaagt attgtaatgt tgcctgttgc
      481 tgtagtagct gttttaccat tctatatgtt ttgggttggt gcttgtgcca cttacggatg
      541 ttaaataact ttttatgtat tctctatacc tgaaaatttt atcggaatgc gattggtgtt
      601 gtgatccaaa aacactttct acctaccagc aagaatggct tctgcacgaa gggtcactta
      661 cccaaaaatt gtatgatgtc gcaaccaatt tcaacgtgga agtagtacaa gaaaagtgga
      721 ttgcaaaaaa cagtgaaaaa acgaccgctt gtaatgaaga tgtttggtcg cgagaagtgt
      781 tactgaaaga tggtgaaaaa aattggattt ttgcccaaac tcttgtgcca agatcaacaa
      841 tcgaaaatat cgctcaaaac gtgttggaat tgggcaatca gccgattggt ttatggctat
      901 tttctcaaaa tcctactcgc accaaattag aatggacaca aacagagaac ggtttattcg
      961 ctcggcgtgg ggtgtatgaa attagagggt attctctgga aattcgagag ctttttttag
     1021 aaacctttcc ttatttataa aaaaatcggt tggtaatgac accaaccgat tttcatctta
     1081 ttttgccaag aactgataaa ttgcagccat atccacttcg ctcaagcctg aatcattggc
     1141 ttgattgtag gttttagtgg cttgctcaat aagcgggtaa gatttgccta atttcgcaat
     1201 ttcttcgctt gccaaattaa aatctttggt catatgtttc atcataaacg caggcggaaa
     1261 ttcattagct tggtacatcg gcacttttgc ttggaagatc gggctgttca taaacgagcc
     1321 tgaaatcatt tcgattactt tctctttcgg aatgccatat tggtcggcga agtttaacgc
     1381 ctcgccatag gcttgaatga agatgcccag tagcgagttg atcatcaatt taatcccacc
     1441 ggctttaccc acttcaccat aatagaaagt ggtggtacta aaggcagcga aaagcggttt
     1501 gagcaactca acggtttctt ctttaccggc agccagtacc aatagtttac ctgcttctgc
     1561 cactttgctt gaaccggaaa ccggagcttc aacaaattct gcaccgtgtt gggctagtag
     1621 agcttctacc gcctggtttt ggcttgggga aatggtgctc atattcacca ctactttgcc
     1681 gtttaattca gccaacactt catcgcttaa cacctgttgc acggcggcat agtctgcaat
     1741 catcaagaaa atcacatcgt aggctttcac caattccaac acagaaggat ataatgtgcc
     1801 gcctgcttca actacctttt ctgcttttgc tgggtcgcgg ttgtaaacgc ccacgttcac
     1861 tccgctattt agcaaatgtt ttgccatcgg ttcgcccatt tgccctaaac caatccaacc
     1921 gactgcttgt gtattaatgt ttttcattat ttttctcctt actgaataaa gcaaattgat
     1981 tattgcgatt ttccctcatc aagtcaaata gagagaactt gaatatagca atggaaaaga
     2041 gtagcatttt agcacttact tttttgaaca ataccaatga aaccaacaat tcttttatac
     2101 gattccggaa tgggcggatt gacgatttac gatgaaatcc gaaaagtgtt accgcacgct
     2161 cactatttat attgctttga taacgctttt ttcccatatt ccgagaagtc ggaagcagtt
     2221 ttaattgagc gagcggtcaa aattgtgcaa aaaattgcag aaaaacaacc gcttgatatg
     2281 gttgttgttg cttgtaatac ggcaagcaca gtggttctgc cggcattgcg tgagcatttt
     2341 aattttccga tagtaggaac tgtaccggca attaaaccgg cagcggcaat ttccgaaact
     2401 aaagtcatcg gcttgctcgc cactaaaggc acggttgctc gcccttatgt tgatgaattg
     2461 ataagaaaat atgctcagga ttgtacggta gaaaaaattg gcacaaccga tttggttgaa
     2521 attgttgaag aaaaacaacg aagcggtaaa gtggatatga accgactaca aaaagtggtc
     2581 gaggcttggc aaggtaagcc ggaattggat acggttatac tcggttgtac ccattttccc
     2641 ttagtcaaag ctgaattaca gcaattattg cccgatgtga aatattttgt tgatccgggt
     2701 gtaggtattg ccaaacgggt gctaagcctt ttggaagaac gcaaatttca acaaaaagcc
     2761 gattttgaaa atcaagcatt ctgcactaaa caaagttctg catttaatat caaacaagac
     2821 gttatgcaga attggggatt taataatctt tcggtgattg atatttaaac aaaacaatct
     2881 gttttgatga aaaaaggttc agttgagcaa aaactttaaa aaagggcttg cactcaattt
     2941 taaa
//
LOCUS       DANLUQ010000200         2898 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.202, whole genome shotgun sequence.
ACCESSION   DANLUQ010000200 DANLUQ010000000
VERSION     DANLUQ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.202"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            56..2095
                     /gene="malQ"
                     /locus_tag="PXB27_002409"
     CDS             56..2095
                     /gene="malQ"
                     /locus_tag="PXB27_002409"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HDL5347148.1"
                     /translation="MSNKQNTAHYFYSPNIKEYASPSAFEKIERLLNTDNDLQKNNQI
                     LPLVKVITQPNAEQQAVEISLNFTNVAKWQIVLENGEVRNGEIKAEQSVLTLPADLPL
                     GYHQLSISTENDEESCSLIVTPPKAFQPKPLEERKKLWGAFLQLYTLRSDNNWGIGDF
                     GDLKQFLANISDYGADFVGLNPIHALFPADPDAASPYSPSFRLWQNIIYIDVSAVEAF
                     QQSEQAQQWFHTKETQQQLAELRAKDFVDYSQVAQLKLKALQFAFEHFSQSSQQQSQT
                     AFDAFIEQAGEPLKIQATFDALHHWISSEFGEKGGWTNWAEEYQSYYSPKVAEFQISQ
                     ATQIRFYMWLQFVANQQLAECNRFAQSLSMPIGFYRDLAVGVTQSGAETWADKALFVQ
                     EASIGAPPDMLAPNGQSWGLSPMHPHILQSRGYQPFIDLVRANMKHCGALRIDHILGF
                     ARMWWVANGESAKEGIYVRYPLEDLLSILALESQRHQCLVIAEALGIVPEGILDSLEQ
                     KGIFAYNVFYFEKAENAFKPLENYPYQAMTTLSTHDLPTIQGYWKGYDFELGEKFDTY
                     PSKAVLRKLKEERSYDRLNIRNAVEKVIALEPNEVGVTIKFTHQLQRYVAHTHSALFG
                     TQPEDWLNMLEPVNIPGTSTEYPNWRRKLSATTEQIFANKDIQQLLKDINEIRKG"
     gene            complement(2163..2894)
                     /locus_tag="PXB27_002410"
     CDS             complement(2163..2894)
                     /locus_tag="PXB27_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HDL5347149.1"
                     /translation="MGCSSFSTKLKQEFFSGWTTFEASWLIVFLAIQIGIFIYQPDTW
                     IATIAAITGILCVVFVGKGKISNYLFGLISVSLYAYISYTFQLYGEMMLNLLVYVPVQ
                     FIGFYFWRKNMTSENTVNNAGVEEVIAKALTAKQWVIVAITTIIGTFLYIELLKYLGS
                     ALAILDGATVVISIVAQILMVLRYREQWALWIIVNIMTISLWTAMYFQNGETSLPLLV
                     MYVMYLCNSIYGYYNWIKLHRKHQQ"
BASE COUNT          929 a          612 c          571 g          786 t
ORIGIN      
        1 attaatcttc acaagatcgt tattttaaca aatacagtaa aggaaaaaag tcataatgtc
       61 aaacaaacaa aatacagctc actattttta ctccccaaat attaaagagt atgccagccc
      121 ctctgctttt gagaaaatag agcggctttt aaataccgat aacgatttgc aaaaaaataa
      181 tcaaattcta ccgcttgtta aagtgattac tcagccaaat gccgagcaac aagcggtcga
      241 aatttcgctg aattttacaa atgtagcaaa gtggcaaatt gtgcttgaaa atggtgaggt
      301 tcggaacggt gaaattaagg cagagcaatc agttctaacc ctgcctgccg atttgccttt
      361 aggctatcat cagctttcta tttcaactga aaatgatgaa gaatcttgct cgctgattgt
      421 aacaccgccg aaagctttcc agcctaagcc attggaagaa aggaaaaagc tctggggtgc
      481 atttttacag ctttatactt tgcgttccga taacaactgg ggaattggcg attttggcga
      541 tctcaaacaa ttccttgcca acatttctga ttatggtgca gattttgtgg gcttaaaccc
      601 tattcacgcc ctctttccgg cagatcctga tgcggcaagc ccttacagcc cgtcctttcg
      661 actttggcaa aatattattt atattgatgt ttcagcagta gaagcctttc aacaatctga
      721 acaggctcaa caatggtttc acaccaaaga aactcaacag caacttgccg aattgcgagc
      781 caaagatttt gtcgattaca gccaagtagc gcaactgaaa ttaaaggctc tgcagtttgc
      841 ttttgagcat ttttcacagt catctcaaca acaatcgcaa acagcgttcg atgcctttat
      901 tgagcaagca ggcgagccat taaaaatcca agcaacgttc gatgctttgc accactggat
      961 ttcatctgaa tttggcgaaa aaggcggctg gacaaactgg gcggaggaat atcaaagtta
     1021 ctattcgcct aaagtagctg aatttcaaat atcgcaggca acgcagatac gtttttatat
     1081 gtggttgcaa tttgtggcaa accagcaatt agcagaatgc aaccggtttg ctcaatcact
     1141 ttcaatgccg attggttttt accgcgattt agccgttggt gttactcaaa gcggagcgga
     1201 aacttgggcg gataaagccc tatttgtgca agaggcgtca atcggtgcac cacctgatat
     1261 gcttgccccg aacggacaga gctggggact ttcgccaatg cacccgcata ttctacaaag
     1321 ccgaggttat cagccgttta ttgatttagt gcgtgctaat atgaaacatt gtggggcatt
     1381 gcgaatcgac catattctcg gcttcgcccg tatgtggtgg gtggcgaacg gtgaatcggc
     1441 aaaagaaggg atttatgtcc gttatccgct cgaagatttg ctctctattt tggcattaga
     1501 aagccaacgt caccaatgtt tagtgattgc agaggcactt ggcattgtgc ctgaaggcat
     1561 tttggactct ctcgagcaaa agggcatttt tgcctacaat gttttttatt ttgaaaaggc
     1621 agaaaacgcg tttaaaccgc tggaaaatta tccttaccaa gcgatgacaa ctctaagcac
     1681 ccacgattta ccgactattc aaggctattg gaaaggctat gattttgagc taggcgaaaa
     1741 atttgacact tacccaagca aagccgtatt gagaaagctg aaagaagagc gttcgtacga
     1801 ccgcttgaat atccgtaatg cggtggaaaa agtgatagca ctcgagccaa atgaagtggg
     1861 agtaacaata aaatttaccc accaattaca acgctatgtg gctcacactc acagtgcatt
     1921 attcggtaca cagcccgaag attggctcaa tatgttagag ccggtgaaca ttccgggaac
     1981 cagcaccgaa taccctaact ggagaagaaa attatccgca accaccgagc agatttttgc
     2041 aaataaagat attcaacaac tgctaaaaga tataaacgaa attcgtaaag gataataaca
     2101 agcggtcatt tttaggtaaa tttttacaaa aaatgaccgc ttgtaatttg tatttattcg
     2161 ctctattgct gatgttttct gtgaagttta atccagttgt aataaccgta aatagagtta
     2221 cataaataca tcacatacat caccaacagc ggcaagctgg tttcgccgtt ttggaagtac
     2281 attgccgtcc ataaagaaat ggtcataata ttcacgataa tccacaatgc ccattgctca
     2341 cggtagcgaa gtaccatcaa aatttgtgcc acaatcgaaa taaccactgt tgcaccatct
     2401 aatatagcta acgcactgcc taaatattta agcaattcaa tataaagaaa tgtgccgata
     2461 atggtggtaa tggcgacaat cacccactgt tttgcggtta aagctttagc aattacctct
     2521 tccacaccag cattatttac cgtgttttcg ctggtcatat ttttacgcca gaaatagaaa
     2581 ccgataaact gcaccggcac atatacaagt aaattcagca tcatttcacc atataactga
     2641 aaggtataag agatataagc ataaagcgaa acggaaatta agccgaatag atagttactg
     2701 atttttcctt taccgacaaa taccacgcat aaaatacctg ttattgccgc aatagttgca
     2761 atccaagtat cgggttgata aataaaaata ccgatttgaa tcgctaaaaa aacaatcaac
     2821 caacttgcct caaaagtagt ccaaccacta aaaaactctt gttttaactt agttgaaaaa
     2881 gatgaacagc ccatattt
//
LOCUS       DANLUQ010000201         2894 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.203, whole genome shotgun sequence.
ACCESSION   DANLUQ010000201 DANLUQ010000000
VERSION     DANLUQ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.203"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..834
                     /locus_tag="PXB27_002411"
     CDS             <1..834
                     /locus_tag="PXB27_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDL5347150.1"
                     /translation="NQSTNQPIVIALAADLGYQTQVETLIKSLCYHHSHLKIYLFQKT
                     FPNEWFDIWCQKLALLSSEIIPVKVCVDFSKYKSLEHINETGFYRYLIPYLDEERVLY
                     LDSDIVVDDNLSEMYWLNFNGIPLYAVEDYVHNHIPAYLDLDLSPYFNSGVLLINNIL
                     WKKYSVCDELVTINEKIVNLTYGDQDALNIYFKGGWGALPIEYNYQVDAILELVLRRE
                     QEELARKNGYLDVIPKIIHYTSKFKPWKKELHTMQISTRKKYWFYYHLEWNDILEQHQ
                     K"
     gene            complement(880..1167)
                     /locus_tag="PXB27_002412"
     CDS             complement(880..1167)
                     /locus_tag="PXB27_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HDL5347151.1"
                     /translation="MKNDKPIECEGCNTFDVGSILDNSETVATIQRTYESENEAKAAL
                     EKFTQKARDVESEPCEISSEITPIESGFELVAHFKFSCQAEVVIFQLGTRF"
     gene            1287..1700
                     /locus_tag="PXB27_002413"
     CDS             1287..1700
                     /locus_tag="PXB27_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PACE efflux transporter"
                     /protein_id="HDL5347152.1"
                     /translation="MTAWERVFHALLFECFAIIFTVILTSWLTSHRMVDLTAVIVMIS
                     VIAMVWNVIFNWGFDKVFTGERVQRGLGIRILHSFLFEGGLLVFTIPLVMYMLDIGWW
                     QAFVMDIGMTLFVLVYSVVFNWVYDHLRLRWVKSG"
     gene            1785..2777
                     /gene="mglB"
                     /locus_tag="PXB27_002414"
     CDS             1785..2777
                     /gene="mglB"
                     /locus_tag="PXB27_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HDL5347153.1"
                     /translation="MKRTILSAFALSVICGVNLSYAQSESRVGVTIYKYDDHFMTQMR
                     KDMQNEAKGFAGLKWFMNDAQNSQVKQLAQIETLLQHKMRVLAVNIVNANESKTIVEK
                     AKAYNVPVVFFNRTPSKQALESYEKAYFVSADPKEAGRIQGELIAKVWKSNPDFDLNK
                     DGKLQFVLLKGEPSDVSAERSQAVVESLNQQGVQAEEIYSDTARWRRTLARNKMNEWL
                     VENRAAEIEVVISNNDEMAIGALDALNAHEKRLPIFGIDALPETLALMKKGEIAGTVL
                     NDSSAQAKAVVKLVSNLAQGKPALKDTQWESHSSKTIYLPHIGIDKENLGQFLP"
BASE COUNT          872 a          450 c          635 g          937 t
ORIGIN      
        1 aatcaatcaa ccaaccaacc aatagttatt gcattagcag ctgatttagg ctatcaaact
       61 caagttgaaa ccttaattaa atcgctttgt tatcatcatt ctcatcttaa gatttatcta
      121 tttcagaaaa cttttcctaa tgaatggttt gatatctggt gccaaaaatt agccttatta
      181 tcgagtgaaa ttattcctgt gaaagtctgt gtagattttt caaaatataa gagtttagag
      241 catattaatg aaacgggttt ttatcgttac cttattccat atctggacga agaacgtgta
      301 ctatatttag attcagatat tgtggttgat gataatttat cagaaatgta ctggcttaat
      361 tttaatggta tacctttata tgcagtagag gattatgttc ataatcatat accggcttat
      421 cttgatttag acctcagccc ttattttaat tctggggtac tattaattaa taatatactt
      481 tggaagaagt attcagtatg tgatgagttg gttactatca atgaaaaaat tgtaaactta
      541 acttatggcg atcaagatgc tctcaatatt tactttaaag gcggttgggg agcattgccg
      601 atagagtata attatcaagt tgatgcaatt cttgaactag tattaagaag agaacaagag
      661 gaacttgcga gaaaaaatgg ctatttagat gtgattccga aaattatcca ctatacgagt
      721 aaattcaaac cttggaaaaa agaactgcat acaatgcaaa tatctacgcg taaaaaatat
      781 tggttttact atcatttaga gtggaacgat attttggaac aacatcaaaa atagctatca
      841 tacaaaagct gattcaagtg tttgaatcag ctttttgtat tagaaacgtg ttcctaattg
      901 aaaaataacc acttctgctt ggcagctaaa tttgaagtga gcaaccagtt caaaaccgct
      961 ttcaatcggg gtgatttcac tgctgatttc acagggttca gattcgacat cacgggcttt
     1021 ttgagtgaat ttttcaagtg ccgctttagc ttcattttcg ctttcataag tgcgctgaat
     1081 agtagcgact gtttcgctgt tatctaaaat agaacctaca tcaaatgtgt tacagccttc
     1141 gcattcaatc ggtttatcgt ttttcattgg ctaatccttc taaattgatg aataatgtag
     1201 gtactttaaa actaaattgc gtaacctgca atcatcagtg aacgattatt tgatttagtt
     1261 ggtattttgc aaagtttgga ggaaaaatga ccgcttggga gcgtgtgttt cacgcgctgt
     1321 tatttgaatg tttcgctatt atttttacgg ttatcttaac ttcttggctg acttctcatc
     1381 gtatggttga tttaaccgca gtgattgtga tgatttcggt gattgcaatg gtgtggaatg
     1441 ttatttttaa ctggggtttt gataaggtgt ttaccggtga gcgagtgcaa cgtggtttag
     1501 gcattcgtat tttgcattca ttcctttttg agggcggttt gctggttttt actatcccat
     1561 tggtaatgta tatgctcgat atcggctggt ggcaggcgtt tgtgatggat attggaatga
     1621 ccttatttgt attagtttat tcggtagttt ttaattgggt gtacgatcat ttacgcctga
     1681 ggtgggttaa atctggctaa attgtgaact agatcacggt ttccttacac aaaattcgct
     1741 atgcttgaat tggttcatat ttggcatagc aagagggaca ttctatgaag cgtacaatct
     1801 tgagtgcttt tgcactttct gttatttgcg gtgtgaatct aagttacgca caatctgaaa
     1861 gcagagtggg ggtaacgatt tacaaatatg atgaccattt tatgactcaa atgcgtaagg
     1921 atatgcaaaa cgaggcgaaa ggcttcgctg ggttgaagtg gtttatgaat gatgcacaaa
     1981 attcccaagt gaagcaatta gcgcaaattg aaaccttact tcagcataaa atgcgtgtgt
     2041 tggcggtgaa tattgtgaat gcaaatgagt cgaaaactat tgtggaaaaa gcgaaggctt
     2101 ataatgtgcc ggttgtcttt tttaaccgca caccgagtaa acaagcgtta gaaagttacg
     2161 aaaaggcgta ctttgtgagc gctgatccta aagaggccgg tagaatccaa ggggaattaa
     2221 ttgcgaaagt gtggaaatca aaccccgatt tcgatctaaa taaagacggg aaattgcaat
     2281 ttgtattgct taagggcgag ccgagtgatg tgagcgctga gcgttctcaa gctgtggttg
     2341 aatcgcttaa tcaacaaggt gttcaggcag aagaaattta cagtgacacg gcgagatggc
     2401 gtagaacatt ggctcgcaat aaaatgaatg agtggctggt ggaaaaccgt gcagctgaga
     2461 ttgaagttgt gatttcaaac aatgatgaaa tggcaattgg tgctttagat gcgttaaatg
     2521 cacacgagaa aagattgccg atctttggta ttgatgcctt gcctgagacg ttagcattaa
     2581 tgaaaaaagg ggaaatagcc ggcacggtat taaatgatag ctctgcacaa gcgaaagcgg
     2641 tggtgaaatt ggtatctaat ttggcacaag gtaagcctgc gctgaaagat acgcagtggg
     2701 aaagccattc ctctaaaacg atttatcttc cacatattgg tattgataag gaaaatttag
     2761 ggcaattttt accttaaatg gaggagcggg gcgttataaa tacgcccctt attgatgata
     2821 acaagcggtc atttttcagt aaaatcttgc aaatcctttc tcttttcttc caacagattt
     2881 gaatagagcg aata
//
LOCUS       DANLUQ010000202         2890 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.204, whole genome shotgun sequence.
ACCESSION   DANLUQ010000202 DANLUQ010000000
VERSION     DANLUQ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.204"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            9..1127
                     /locus_tag="PXB27_002415"
     CDS             9..1127
                     /locus_tag="PXB27_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HDL5347154.1"
                     /translation="MKKTLVALAVTAFAASASATTVYDAEGSKVDFDGSLRIVLDRTN
                     SKELDNHNVVKTKKDAHTGIRNTGSRFGVRVKHDLAQDFYALGRVEFRFNKNTSDDGF
                     GDLYTKRAYVGLGSKQYGELTFGRQVTIADDLSQSVDYNYGINPKGDYIQTDGNQVIR
                     YNYRGVENLHVSANYNFAQDRKDGKVLANKLQNAYGIGALYTVDNWDLRAAYGHTNYK
                     TATDSTKHRKDGVLGSVGYKFGDLKLSVDTGYSREKGLAVTDDNGVTTFYNGVKSNAF
                     YVSPNFEYQVVPASKVYGNYLYERVKSKSNGSTYAAGKEKTHGFLLGVDYQFHKQVVA
                     FVEGSYTRTKDYDKVTNGYEYSGKSTNKAIGVGMRVYW"
     gene            complement(1280..2644)
                     /gene="glmU"
                     /locus_tag="PXB27_002416"
     CDS             complement(1280..2644)
                     /gene="glmU"
                     /locus_tag="PXB27_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012262868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDL5347155.1"
                     /translation="MTQLSVVILAAGKGTRMYSDLPKVLHPVAGKPMVKHVIDTAKQL
                     DAQQIHLIYGHGADLLKARLSEEPVNWVFQAEQLGTGHAMQQAAPFFADDENIVMLYG
                     DAPLITKATLERLIAAKPENGIALLTVELENPTGYGRIIRENGSVVAIVEQKDANEAQ
                     LKVKEVNTGVMVASGASFKKWLKNLNNNNAQGEYYITDVIAMANQEGFKVQAVQATEF
                     MEVEGANNRLQLAALERFYQKREAEKLLLAGVTIIDPSRFDLRGTVTHGKDVQIDVNV
                     ILEGEIKLGNNVKIGAGCVLKNCEIGDNVEIKPYSVIEDSIVGAKSAIGPFSRLRPGA
                     ELAEETHIGNFVEIKKATIGKGSKVNHLTYVGDAEIGKECNIGAGVITCNYDGANKFK
                     TIIGDNVFVGSDSQLIAPVTIASGSTIGAGATVTKDIAENELVISRVPQRHIQGWQRP
                     TKKK"
BASE COUNT          829 a          526 c          563 g          972 t
ORIGIN      
        1 tgaaaattat gaaaaaaaca ttagttgcat tagcagtaac cgcgttcgca gcgtcagcat
       61 cagcaaccac cgtttacgat gcagaaggtt caaaagtaga ttttgatggt tctttacgta
      121 ttgttcttga tagaacaaat tctaaagagc tagacaatca taatgttgtt aaaactaaaa
      181 aagatgcaca cacaggaatc cgtaacactg gttcacgttt tggtgtaaga gtaaaacatg
      241 atttagctca agatttctat gcattaggtc gtgttgagtt ccgttttaac aaaaatacca
      301 gtgatgatgg ttttggtgat ttatatacta aacgtgctta tgttggttta ggtagtaagc
      361 aatatggtga attaactttt ggtcgtcaag taacgattgc tgatgattta agtcaatcag
      421 tagattacaa ctacggtatt aatccaaaag gtgattatat tcaaaccgat ggtaaccaag
      481 ttattcgtta taactaccgt ggtgttgaaa acttacatgt aagtgcaaac tataactttg
      541 cacaagatcg taaagatggt aaagtattag caaataaact tcaaaatgca tacggcattg
      601 gtgcattata cactgtagat aactgggatt tacgtgcagc ttatggtcat acaaactaca
      661 aaacagctac tgatagcact aagcaccgta aagatggtgt attaggttct gttggttata
      721 aatttggtga cttaaaatta agtgttgata caggttattc tcgtgagaag ggtttagctg
      781 ttacggatga taatggtgta actacattct ataatggtgt taaatcaaac gctttctatg
      841 tatctccaaa ctttgaatat caagtagtgc ctgcatctaa agtatatggt aactacttat
      901 atgagcgtgt gaaatctaaa tcaaatggtt ctacatatgc tgctggtaaa gaaaaaactc
      961 atggtttctt acttggtgtt gattaccaat tccataaaca agtagttgct tttgttgagg
     1021 gttcttatac tcgtactaaa gactatgata aagtaactaa tggttatgaa tactcaggca
     1081 aatcaactaa caaagctatc ggtgttggta tgcgtgtata ctggtaatca attgattttc
     1141 taaattgata aacccctaac aatatttatt gttaggggtt ttgttttaaa tatattgttc
     1201 attatccttt ttaaagtgta tggctttgct gttattggca aagccaattt ttaaatataa
     1261 taacttttga ccactggcac tatttctttt tagtcgggcg ttgccagcct tgaatatggc
     1321 gttgtggtac acggctaatt accagttcat tttccgcaat gtctttagtg actgttgcac
     1381 cggcaccaat ggttgaaccg ctggcaatcg tgaccggggc aatgagttgg ctgtctgagc
     1441 cgacaaatac gttatcgcca ataatggttt tgaatttatt cgcaccatca tagttacaag
     1501 tgattacacc tgctccgatg ttacattctt tgccgatttc cgcatcgcct acataagtga
     1561 gatgatttac ttttgaacct ttgccgatgg tagctttttt gatttccaca aagttaccga
     1621 tatgggtttc ttccgctaat tctgcccccg ggcgtaagcg agagaatgga ccgatagcgg
     1681 attttgcccc gacaatcgaa tcttcaatca cagaataagg tttaatttca acattatcgc
     1741 caatttcgca gtttttcagt acgcagcctg caccaatttt tacattgtta ccgagtttga
     1801 tttcaccttc tagaatgaca ttcacatcga tctgaacatc ttttccgtgg gtgacagtgc
     1861 cacgaagatc aaaacggctt gggtcgataa tagttacacc agccaataac agtttttccg
     1921 cctcacgttt ttggtagaac cgttcaagtg cagcaagttg caaacggttg tttgcccctt
     1981 ctacttccat aaattcagtt gcctgaaccg cttgtacttt aaagccttct tggtttgcca
     2041 ttgcgatcac atcagtgatg taatattcac cttgagcatt gttgttattt aggtttttca
     2101 accatttttt gaagctcgca ccgcttgcta ccatcacgcc ggtattcact tctttgactt
     2161 taagctgtgc ttcattggca tctttttgct caacaatcgc taccacagaa ccattttcac
     2221 gaataattcg tccgtagcct gtcgggtttt ccaattcaac ggttagtaat gcaataccat
     2281 tttccggttt ggctgcgatt aaacgctcta aagtggcttt tgtgattagg ggggcatcac
     2341 cataaagcat tacaatgttt tcatcatcag cgaaaaaagg agctgcttgt tgcattgcat
     2401 gtcctgtgcc taattgttcg gcttggaata cccagtttac aggctcttcg cttaaacgtg
     2461 cttttaacag atcggcaccg tgtccgtaaa ttaaatgaat ttgctgggca tctagctgtt
     2521 tggcagtatc aataacgtgt tttaccatgg gtttaccggc aactgggtgt aacactttgg
     2581 gtaaatctga atacatacga gtgcctttac cggcagctaa aataacaacg ctcagttgag
     2641 tcatatagaa tccttatttg actaaataga tgaaggctta tagtaacgtg taaattgcta
     2701 taagtgatat ttttgtaata ttttaacctt tttataagtg gatttcatcg acaagcaaaa
     2761 gaaatttgtc tgtttgttat ctttttctat tatgtcgatt cttatcttgt ggaaaactgg
     2821 tataaatagg tagcacttat ttgtgctatc tctctacaat agtgtatatc tttgctaaaa
     2881 tattaaatta
//
LOCUS       DANLUQ010000203         2873 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.205, whole genome shotgun sequence.
ACCESSION   DANLUQ010000203 DANLUQ010000000
VERSION     DANLUQ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.205"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..697)
                     /locus_tag="PXB27_002417"
     CDS             complement(<1..697)
                     /locus_tag="PXB27_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HDL5347156.1"
                     /translation="MRSMFKKFFGKNKSQSAVKSDTIFSIEQDNEGWLYPEKASQLLN
                     TELRQKYLGLLWQQVSMNRQMFEVLYQQPIERYAEMVQLLPASESHHHSHIGGMLDHG
                     LEVIAFSAKVRQSYILPPNVAPEEQSKQRDAWTAAAIYLALVHDIGKVVVDIEITLKD
                     GSRWFAWNGQPPQPYKFKYIKGRDYHLHPVLGGFLANQLIPKSAFDWLGQYPEVISSL
                     MYAMSGHNDKAGLL"
     gene            complement(801..1265)
                     /locus_tag="PXB27_002418"
     CDS             complement(801..1265)
                     /locus_tag="PXB27_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC family protein"
                     /protein_id="HDL5347157.1"
                     /translation="MMNTQQPKFDLYQHITDRIIAALEQGTAPWLKPWSHPECNMALP
                     RNAVSNRLYSGINTLLLWIATAEHGYKQCKWITAKKANELGGYIRKGEKATVVMNYRP
                     NEREKLDEEGNIVYDEEGNPEMEQHFFIAKHNLFNLDQCENLPAELYDPVIP"
     gene            1474..1737
                     /locus_tag="PXB27_002419"
     CDS             1474..1737
                     /locus_tag="PXB27_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme-like superfamily protein"
                     /protein_id="HDL5347158.1"
                     /translation="MRPVTRNNRGTSFDPRHNKYINTLATALLARWHIEQFSERNIPV
                     NDENLYMAHNIGLDGFHRALLGQSTRKDIENMRKNGMKPWMSI"
     gene            complement(1821..2846)
                     /locus_tag="PXB27_002420"
     CDS             complement(1821..2846)
                     /locus_tag="PXB27_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HDL5347159.1"
                     /translation="MTAGDTPHDTLKTLVGALKDTREEARKANERGDRFEQEVLRLQA
                     REAEVDSRIAGEVESRVESRLAAAWDGFNRQIEELKGRVQSGNQQQKEAEAIPVGGAS
                     SGFGTESAGQTGNVDNNGIRWVDPDDMVVTDEQGKPVTGNYSGKTKTGFPSPFKAEGE
                     SSNTGTALNNNAGTENMTGNTQIQQIKRTPYYTIPENSTLMGSTAMTALLGRVPIQGA
                     VTDPFPFKVLIGRENLIANGIDLPDVEGAIVSGTSTGDWTLSCVRSEVTSITFVFADG
                     RISNGRASGESRAVKRNQASNLTPFCLTVSLSGFNDIPHSDGNPGFLTMSAVDYVDVY
                     LMSHLAR"
BASE COUNT          801 a          646 c          560 g          866 t
ORIGIN      
        1 ataaaagccc ggctttatcg ttatgcccag acattgcata catcagtgaa gaaatgactt
       61 ctggatactg tcccagccaa tcaaacgcag acttgggaat gagctgattt gcaaggaatc
      121 ctcctagcac gggatgaaga tggtagtctc gacctttgat gtatttgaat ttataaggtt
      181 gaggtggctg tccattccaa gcaaaccaac gagagccgtc tttaagagtg atttcaatat
      241 caaccacaac tttgccaata tcatgaacca aggcaagata aattgcggca gcagtccaag
      301 catctcgttg ctttgattgt tcttcgggag ctacattcgg gggcaaaata tagctttgac
      361 gaactttagc tgaaaaagca ataacttcta aaccgtgatc aagcattcca ccgatatgtg
      421 aatgatggtg tgattcagaa gctggtaata gttgaaccat ctcagcgtaa cgttctattg
      481 gctgctgata aagtacctca aacatttgac ggttcattga tacctgttgc caaagtagcc
      541 caagatattt ttgtcgtaat tctgtattaa gtaattgaga ggctttttcc gggtataacc
      601 acccttcatt atcttgctca atagaaaaaa tagtatcgga tttgaccgca ctttgagatt
      661 tatttttacc aaaaaatttt ttgaacattg aacgcattgg aagagaccga aaatatgaat
      721 gctgtattga atgaaagccg tttagccctt tgcccttttg ggtaagacct ttccccttgg
      781 aaaggttact cttatccccc ttaggggatt acaggatcgt acagttccgc cggtaaattt
      841 tcacactggt ctaaattgaa cagattgtgc ttagcaatga aaaagtgttg ttccatttcc
      901 ggatttcctt cctcgtcata cacaatattt ccctcctcat cgagtttttc tctctcatta
      961 ggacggtagt tcatcacaac agtagctttt tcccctttac ggatatatcc gccgagttca
     1021 ttggcttttt tcgccgtaat ccacttacac tgtttgtagc cgtgttcagc ggtagcaatc
     1081 caaagcaata acgtattgat accggaatac agacggttag aaaccgcatt gcgaggtaat
     1141 gccatattgc attccggatg gctccacggt tttaaccacg gagctgtacc ttgttccaat
     1201 gcagcaatga ttctatcagt aatatgttgg tataaatcaa atttaggttg ttgagtgttc
     1261 atcatgtgtt tctccaaagt ttgggtaaaa aaatggggaa acacggttac cctatgggga
     1321 acttgtttcc ccgatgggta gtttataaaa tttcaatggc cacaattttt ctagaatcat
     1381 caaagccaac agaactgcct atggcgtagt aattacgaat agtctgttca tcggcattga
     1441 ttagcagata cctatcgagg aagagagatt gggatgagac cggtaacgag aaataatcgt
     1501 ggaaccagtt ttgaccctcg tcacaataaa tacatcaata cattggcaac cgcattattg
     1561 gcacgttggc atattgagca attctctgag cgtaatatcc cagtcaatga tgaaaatctc
     1621 tatatggctc acaatattgg cttagatggt ttccatcgag cgttattagg gcaatctact
     1681 cgtaaagaca ttgagaatat gcgtaagaat gggatgaaac cttggatgtc catttagctc
     1741 tgttttcaaa tgacctaatc cagtttgttc cggagagagt accgaaattt tgcactcttt
     1801 catccgtggt gtatcaaact tcaacgtgct aaatgagaca ttaaatatac atcaacataa
     1861 tctaccgcac tcatcgttaa gaaaccggga ttcccatcag aatgaggaat atcgttaaag
     1921 cccgataagg agacggttaa acaaaatggt gttaaattac ttgcttgatt tcgtttcaca
     1981 gctctgcttt ctccactcgc tcgcccattc gaaatacgac cgtctgcaaa cacaaaggta
     2041 atgctcgtta cctcagaacg aacgcaagat agcgtccaat ctccggttga tgtaccggat
     2101 acaatcgccc cctcaacatc aggtaaatca atgccattgg caattaaatt ttctcgcccg
     2161 attaatactt taaacggaaa cggatctgtc acagcacctt gaataggtac acggcctaac
     2221 aatgccgtca tcgcagtaga acccattaaa gtcgaatttt cgggaatagt gtaataaggt
     2281 gtacgcttta tttgctgtat ctgagtatta ccagtcatat tttctgtccc agcattatta
     2341 tttaatgctg tcccagtatt tgaactttcc ccctctgctt taaaagggct tggaaagccg
     2401 gtttttgttt tcccggaata attgcctgta accggtttac cttgttcatc ggttaccacc
     2461 atatcatcag gatcgaccca gcgaatgcca ttgttatcta cattccctgt ttgtcctgct
     2521 gattcagtcc caaagccaga agaagcccct cctacaggga ttgcctctgc ttctttctgc
     2581 tgctgattac cactttgaac ccgacctttc agttcttcga tttggcgatt aaatccatcc
     2641 caagcagcag ccaaacggct ttctacacga ctttctactt cacccgcaat acgagaatcc
     2701 acttcggctt ctcttgcttg taagcgtaat acctcttgct caaagcgatc accacgctca
     2761 tttgctttac gagcttcttc acgagtatct tttaatgctc ctaccaacgt ttttaatgta
     2821 tcgtgaggcg tatctcctgc agtaatacct aatgtattcg cctcctcatc act
//
LOCUS       DANLUQ010000204         2831 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.206, whole genome shotgun sequence.
ACCESSION   DANLUQ010000204 DANLUQ010000000
VERSION     DANLUQ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.206"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..535
                     /locus_tag="PXB27_002421"
     CDS             <1..535
                     /locus_tag="PXB27_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347160.1"
                     /translation="KWKYILPLIAFTALILFLAIVFPENRYVEHALEPVINLLSGTGK
                     VSSSTDNLVKNHLFLPTLNQFISGDGLYYTETGGYYGGSDSGYIRQVLYGGVSYMLAC
                     FAFTYYFVRKVAVNWFEGSWIFTLSTIGILAILNIKADTFAFPGLMMLFLMFLSLFGT
                     SGRNVTFFCKNKEAEYV"
     gene            528..1493
                     /locus_tag="PXB27_002422"
     CDS             528..1493
                     /locus_tag="PXB27_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005578999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL5347161.1"
                     /translation="MFSIIVPSYNRNQEINALLESLKQQTAYNFEVIIVDDCSKIPVS
                     ETLGILNEFNDISTKEKVRELLTRDSLSHIYSYPFDVKVVRNNPNVGAAQSRNVGATN
                     ATQEWLLFLDDDDRFMPEKCERLAAEIANNPTANFVYHPAKCEMVNEGFTYVTKPFKH
                     KSELTKENILKANKIGGMPMIGLKKSLFCKIGGLSDRLRSLEDYDFLLKLLEEPSFEP
                     LYVDEALTYCTFHTKRSSVSTDTTNTEKAIEYIQQTYIKTPEANNFKLNSLYILSYPH
                     VMNLSRKAAGYYFEMFKQTKNIKQLIIALVIFISPKLAINLKRFM"
     gene            1493..2293
                     /locus_tag="PXB27_002423"
     CDS             1493..2293
                     /locus_tag="PXB27_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_162680708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL5347162.1"
                     /translation="MKFSVLMSLYIKEQPQYLRECFESLKVQTVQADEIVVCFDGAVT
                     PELEAIVEEYSAILPINAVKFPQNRGLGKTLNDGLTYCKNEWVFRMDTDDICMPERFE
                     KQVAFIEQNPETVIFGGQIAEFGQDIHDLVAYRNVPTTVEEIVRFTRLRCPFNHMTVA
                     YQKSKVLEVGGYQDLQEDYYLWIKLIAQFKQVANLPDLLVYARVGNGMVGRRRGMAQA
                     EAEWRLYKLKNRCQIHDPISGFGIFIMRAIPRLLPVSALKKVYGLLRK"
     gene            complement(2330..2827)
                     /gene="msrA"
                     /locus_tag="PXB27_002424"
     CDS             complement(2330..2827)
                     /gene="msrA"
                     /locus_tag="PXB27_002424"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007525436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL5347163.1"
                     /translation="MREIYLAGGCFWGVESFMQRIKGVIKAQSGYANGNTDNPSYQEV
                     CAGSGHAEVVKVTYDANQISLAKLLDYFFKVIDPVSINKQGGDIGIQYRTGIYYTDPQ
                     DEPVVTAALAQLQTQFDQPLAVENQMLEQYFPAEEYHQEYLVKNPNGYCHIDIQLMNE
                     ILKNQ"
BASE COUNT          840 a          498 c          594 g          899 t
ORIGIN      
        1 taaatggaaa tatatattgc cactgattgc cttcactgcg ttaattctat ttttggcgat
       61 tgtttttccg gaaaaccgtt atgttgaaca cgccttagaa ccggtaatta atttattgtc
      121 cggcaccgga aaagtcagct cttcaaccga taatttagtg aaaaaccatc tgtttttacc
      181 gactttaaat cagtttattt ccggtgatgg tttatattac accgaaactg gtggctatta
      241 tggtggatcg gattccggct atattcgcca agtattatat ggtggagtaa gttatatgtt
      301 ggcgtgtttt gccttcactt actattttgt acgcaaagtg gcggtgaatt ggtttgaagg
      361 cagttggatt tttaccttat ccaccatcgg cattttagct attttaaata ttaaagcaga
      421 tacctttgct ttcccggggc tgatgatgct gtttctaatg ttcttgtcgc tgtttggaac
      481 aagcggtcga aatgtaacat ttttttgcaa aaacaaagag gctgaatatg tttagtatta
      541 ttgtgccttc ttataaccgt aatcaagaaa tcaatgcgtt gcttgagagt ttaaagcaac
      601 aaactgcata taattttgaa gtgatcattg ttgatgactg ctcaaaaata ccggtcagtg
      661 aaactttagg aattttaaat gaatttaacg atatttctac taaagaaaaa gtaagagaat
      721 tacttactag agatagttta tctcatattt atagctatcc atttgatgtg aaagtggtgc
      781 gaaataaccc aaatgttggg gcggcacaaa gccgaaatgt aggggcaact aatgccacac
      841 aagaatggct actgttttta gatgatgatg accgctttat gcctgaaaaa tgcgaacgtt
      901 tagcggcaga gattgcgaac aatccaactg ccaattttgt gtaccacccg gcaaaatgtg
      961 aaatggtaaa tgagggtttt acttatgtca ccaagccgtt taaacataaa agtgagctaa
     1021 ctaaagaaaa cattctaaaa gcgaataaaa ttggcggaat gccgatgatt ggcttgaaaa
     1081 aatcactctt ttgtaaaatt ggtggattat ctgaccgctt gcgatcactt gaagattatg
     1141 atttcttact gaaattattg gaagaaccga gttttgagcc attgtatgtg gacgaagcct
     1201 tgacctattg tactttccat accaaacgct ccagtgtttc aaccgatacc actaatacag
     1261 aaaaggctat tgagtatatt caacaaacgt atatcaaaac acctgaggcg aataatttta
     1321 agctcaattc gctttatatt ttaagttatc cacacgtaat gaatttatcc cgcaaggcag
     1381 ccggttatta ttttgagatg tttaaacaga ctaaaaatat caaacaactg attattgcgt
     1441 tggttatttt tatttcgccg aagttggcga ttaatttgaa aaggtttatg taatgaaatt
     1501 ttccgtttta atgtcgttat acattaaaga gcaaccgcaa tatttaagag aatgttttga
     1561 aagcttgaaa gtacaaactg tgcaggctga tgaaatcgtt gtttgttttg atggtgcagt
     1621 tacgcctgaa ttagaggcga ttgtagaaga atatagtgca attttgccga ttaatgcggt
     1681 gaaattccca caaaatcgag gtttaggtaa aacgctaaat gatgggctaa cctattgtaa
     1741 gaatgaatgg gttttcagaa tggatacaga cgatatttgt atgccggaac gtttcgagaa
     1801 acaagtggca tttattgaac agaatcctga aaccgtgatt tttggcggcc aaattgctga
     1861 atttgggcaa gatattcacg atcttgtcgc ttaccgcaat gtaccaacca cggtggagga
     1921 aatcgtcaga ttcactcgct tgcgttgccc gtttaaccat atgacggttg cttatcagaa
     1981 atccaaagtg ttggaagtcg gtggctatca agatctgcaa gaagattact atttatggat
     2041 caaactcatt gctcaattta aacaagtggc aaacttgccg gatttattag tgtatgcccg
     2101 agtcggtaac ggaatggttg gcagacgccg tggaatggcc caagcagagg cagaatggcg
     2161 tttatataaa ttaaaaaacc gctgccagat tcacgatccg atttcaggtt ttgggatttt
     2221 cataatgcgt gcaattcctc gtttattacc ggtttcagca ctgaaaaaag tgtacggttt
     2281 gctcagaaaa taaagctaaa aaaaccacag attgctctgt ggttttttgt tattgattct
     2341 tcaaaatctc attcattaat tgaatatcaa tatggcagta gccgttcggg ttttttacta
     2401 aatattcttg gtggtactct tcggcaggga aatattgctc taacatttgg ttttccactg
     2461 ccagcggctg atcaaattgg gtttgtaatt gtgcaagtgc ggcggttacc accggttcgt
     2521 cttgtgggtc ggtataataa ataccggtgc ggtattgaat accgatgtcg ccaccttgtt
     2581 tgttaatgct caccgggtct attactttaa agaagtaatc tagtaatttt gccaagctaa
     2641 tttgattggc atcataagtc acttttacca cttcagcgtg tccgctaccg gcacacacct
     2701 cttgatagct tgggttatcg gtattaccgt tggcatagcc tgattgtgcc ttaatgacac
     2761 ctttaatccg ttgcataaaa gattccacgc cccagaaaca gccaccggct aagtaaattt
     2821 cacgaatatt t
//
LOCUS       DANLUQ010000205         2818 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.207, whole genome shotgun sequence.
ACCESSION   DANLUQ010000205 DANLUQ010000000
VERSION     DANLUQ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.207"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            27..>2818
                     /locus_tag="PXB27_002425"
     CDS             27..>2818
                     /locus_tag="PXB27_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S6"
                     /protein_id="HDL5347164.1"
                     /translation="MKTKTFTRSYLASFVTIVLSLPAVASVVRNDVDYQYFRDFAENK
                     GPFSVGSMNIDIKDNNGQLVGTMLHNLPMVDFSAMVRGGYSTLIAPQYLVSVAHNTGY
                     KNVQFGAAGYNPDSHHYTYKIVDRNDYEKVQGGLHPDYHTPRLNKLVTEVVPAAVTNA
                     GTSIKPYLNEERFPMFLRAGSGTQALRGKESNKTTGIAGAYEYLTGGTTLQLSKSSPD
                     HWLDYSSNLYQVSYGPLSTYALPGDSGSGSYAYDMNEKRWVLVGVLNFYNGMDNQFNR
                     SAIIRKDFHEKKFAEDIAGTINNTVQNAQFNWTAQGKSSSLSQSNNVQKLNVDLKDSS
                     IANQNTSLPQENHGKTINFNGKDATIVLKQDIDQGAGALNLNANLTIRPETDQTWQGA
                     GIIVGKDKKVNWQVKNPQGDRLSKLGEGTLYVNGRGQNLGDISVGDGIVILNQQADHQ
                     GRKQAFNTVGIVSGRPTVVLGSADQVNPDNIYFGFRGGRLDLNGNSIAFKRIQNSDKH
                     ARIVNHNRDHISTLIIQGQDPLTSNDLIWGKWASNSPADIYEYTNPYQNKRKDYFRLK
                     GNSRVYYPTNATSNDHWEFLSSNREQAIQKILDAKNLRQRYDTFNGFIGEDASNKTNG
                     ILNVVFDTKTEVNTEQDKLKNIYTMSGGFNLNGELTLKGGTLLLSGHPTPHAYDIKNK
                     HDVVRENDWQDSHFTAKNITVNKMAQLYIGRNVNEVNSHFTATDKAKLNLGFINRSTP
                     SCYDSEYTGTTHCEVQAVISDNIFANLATTAIKGNVKLQNHSQLNLGKANLTGSVQAD
                     QTTHITLANHSHWLNNGTSQIGHLTMEKGSILSLNDKFATTEIPVRFNKMIIQGNLKG
                     NGRINYTANLAKGESDHLQVDGIAEGNFVLAVRNSTTEANPKSSLNLLSLKNSNQEGN
                     KASISLENNYVDLGTYR"
BASE COUNT          986 a          537 c          518 g          777 t
ORIGIN      
        1 tacttataag gataaacttc tcatttatga agaccaaaac atttactcgt tcttatcttg
       61 cttcttttgt aacaatcgta ttaagtttac ctgctgtagc atctgttgta cgtaatgatg
      121 tggactatca atacttccgc gattttgccg aaaataaagg accattttca gttggttcaa
      181 tgaatattga tattaaagac aacaatggac aacttgtagg cacgatgctt cataatttac
      241 caatggttga ttttagtgct atggtaagag gtggatattc tactttaatt gcaccacaat
      301 atttagttag tgttgcacat aatactggat ataaaaatgt tcaatttggt gctgcaggtt
      361 ataaccctga ttcacatcac tatacttata aaattgttga ccgcaatgat tatgaaaagg
      421 ttcaaggagg gttgcaccca gactatcata ctcctcgatt aaataaatta gtaacagaag
      481 ttgtgcctgc cgcagtcacc aatgcaggta catctattaa accctactta aatgaagaac
      541 gcttccctat gtttcttcgt gctggttcag ggacacaagc gctaagagga aaagaaagta
      601 ataaaacaac tggaatcgct ggtgcttatg aatatcttac tggcggtacc acattacaat
      661 tatctaaaag ctcccctgat cactggttag attattcaag taacctttat caagtaagct
      721 atggaccact ttcaacctat gcactacctg gtgatagtgg ttcaggttct tacgcctatg
      781 atatgaacga aaaacgatgg gtattagttg gtgtgctcaa tttctataat ggtatggata
      841 atcaattcaa ccgctctgcg attatccgta aagatttcca cgagaaaaaa tttgccgaag
      901 atattgcagg aacaatcaat aataccgtac aaaatgcaca attcaattgg actgctcaag
      961 gtaaatccag ctctcttagt caatcaaata atgtgcaaaa actcaacgtt gatctaaaag
     1021 atagtagcat tgcaaaccaa aacacttctc tgccacaaga aaatcacggt aaaaccatta
     1081 attttaatgg taaagatgca actattgtac taaaacagga tattgaccaa ggtgcaggtg
     1141 cattaaatct gaacgctaat ctcactattc gtcctgaaac agaccaaact tggcaaggtg
     1201 caggtattat cgtcggtaaa gataaaaaag tgaattggca agtaaaaaat ccacaaggcg
     1261 atcgtttatc taaactcggg gaaggaacac tctatgtaaa tggacgtgga cagaatcttg
     1321 gcgatatcag tgtaggtgat ggtattgtaa tacttaacca gcaagccgat caccaaggaa
     1381 gaaaacaggc ctttaataca gtaggaatcg taagtggtcg ccccactgtt gtgctaggta
     1441 gtgcagatca agttaatccc gataatattt actttggatt tcgcggaggt cgtttagacc
     1501 taaacggtaa cagcatcgcc tttaaacgta ttcaaaacag cgataaacat gctcgtattg
     1561 taaaccacaa tcgcgatcac atttctacct taataataca aggccaagat cctctcacta
     1621 gtaatgatct tatatgggga aaatgggcaa gtaatagccc agcagacatt tacgaatata
     1681 ccaatcctta tcaaaataaa cgcaaagatt acttccgtct gaaaggtaat tcgagagtat
     1741 attatccaac gaatgctaca agtaacgatc actgggaatt tctttccagt aaccgcgagc
     1801 aagcaataca gaaaatccta gatgccaaaa acttaagaca gcgctatgac acgtttaatg
     1861 gttttatagg ggaagatgct tccaataaaa ctaatgggat attaaatgtc gtgtttgata
     1921 caaaaacaga agtaaataca gaacaagata aattaaagaa tatctacaca atgtcgggag
     1981 gatttaacct taatggtgaa ctcaccctta aaggtggtac attgttgctt tctggtcacc
     2041 caacgccaca cgcttatgat attaagaata agcatgatgt tgtgcgtgaa aacgattggc
     2101 aagacagcca ttttactgct aaaaatatca cggtaaataa aatggcacaa ctctatatcg
     2161 ggagaaatgt caatgaagta aatagtcact ttactgcgac tgataaagcc aaactcaatt
     2221 taggatttat taatcgttca acgccaagtt gctatgattc tgaatacaca ggcactacac
     2281 attgtgaagt gcaagcggtc atttccgata atatttttgc aaatctagca acaaccgcca
     2341 ttaaaggtaa tgttaaatta caaaaccata gccaattaaa tttaggcaaa gcaaacctca
     2401 ctggttctgt acaagctgat caaacaactc atatcacttt agcaaatcac agtcactggt
     2461 taaacaatgg tacgagccag attgggcatc ttacaatgga aaaagggtcg atccttagcc
     2521 taaacgataa atttgctacc acggaaatcc cagtccgatt caacaagatg atcatccaag
     2581 gtaatctaaa aggtaatgga cgaattaact ataccgcaaa tttagccaag ggcgaatctg
     2641 atcatctcca agttgacggt attgctgaag gaaattttgt ccttgccgtt agaaatagca
     2701 caactgaagc aaatccaaaa agctcattaa acctactaag cttaaaaaat agcaaccaag
     2761 aaggcaataa agcttctatt tctctagaaa ataattatgt tgatctaggt acttatcg
//
LOCUS       DANLUQ010000206         2792 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.208, whole genome shotgun sequence.
ACCESSION   DANLUQ010000206 DANLUQ010000000
VERSION     DANLUQ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.208"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..321)
                     /locus_tag="PXB27_002426"
     CDS             complement(<1..321)
                     /locus_tag="PXB27_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL5347165.1"
                     /translation="MWVRLGDICLKITDGTHHSPPNIDKSDFLYITAKNIKKDGLDLS
                     KISYVTKEIHNEIFSRCNPEKGDILYIKDGATTGVSIINTLNEPFSMLSSVALIKTSQ
                     EIDNE"
     gene            499..2775
                     /gene="gshAB"
                     /locus_tag="PXB27_002427"
     CDS             499..2775
                     /gene="gshAB"
                     /locus_tag="PXB27_002427"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HDL5347166.1"
                     /translation="MKLQKLIRENHLALLFQQGNFGLEKESQRVDRNGNIVTTPHPAV
                     FGNRSYHPYIQTDFAESQLELITPPNAKLEDSFRWLSAIHEVVLRSLPEEEYIFPLSM
                     PAGLPPEEQIQEAQFDNPEDVQYRQHLSKNYGKYKQMVSGVHYNFQLSTEFVEKAFAL
                     QTEYSELKAFQNALYMKLANNFLRYQWILLYLLAATPTVESQYFYEKSPLARGQLVRS
                     LRSSPYGYVNSEKVVVNHDSLESYVESLEANVANGLLIAEKEFYSNVRLRGGKKAREL
                     LENGVKYAEFRLFDLNPFAPYGIELNDAKFIHYFLLGMLWLEETSGQKEVEIGKQKLY
                     QVALEDPRTETAFRAEGEAILNQLLAMLEELSAGQESLDLVREKLAQFADPSKTLNGR
                     LIAAIEEVGSYKALGAKLAQTYKAQAFERFYALSAFDNMELSTQALFFDLIQQGIEVE
                     VLDENDQFLALKFGDHLEYVKNGNMTSRDQYISPLIMENKVVTKKVLAKAGFNVPKSV
                     EFTNLEQAVAHYPLFEGKAVVIKPKSTNYGLGITIFQQAVKNREDFAKAVEIAFREDK
                     EVMVEDYLVGTEYRFFVLGDETLAVLLRVPANVVGDGIHSVKELVERKNDDPLRGDGS
                     RSPLKKIALGEIEQLQLKEQGLTVDSVPAKGQTVQLRANSNISTGGDSIDMTDQMHES
                     YRQIAVGIAQAMGAKVCGVDLIIPDLTKPAEPNLSSWGVIEANFNPMMMMHIFPYQGK
                     SRRLTKAVIKMLFPEIGN"
BASE COUNT          845 a          569 c          594 g          784 t
ORIGIN      
        1 ctcattatct atttcttgtg aggtcttaat taaggcaaca ctggataaca tactaaatgg
       61 ctcattaaga gtatttataa tacttactcc agttgtggct ccatctttaa tatatagaat
      121 atctcccttt tcaggattac aacggctgaa aatttcatta tgaatttctt ttgttacata
      181 gctaattttt gataaatcta atccatcttt ctttatattt ttagctgtaa tataaagaaa
      241 atcactctta tcgatatttg ggggagaatg atgagttcca tctgtaattt ttaggcatat
      301 atcccccaac ctcacccaca cccaactctc aggaatctca aaaggtggtt caatggtata
      361 aggggcttta tccgaagcag tatttctgtt ataaccaaat catctacttc attccaataa
      421 actaaaaccc actatttcac taagtctttc atttgtgtta ttgtgccgat acattttttt
      481 gactacaaag gatagatgat gaaactacaa aaacttatcc gagaaaatca tttagcattg
      541 ctgtttcaac aagggaattt tgggcttgag aaagagagcc aacgggttga tcgcaacggc
      601 aatatcgtta ctactcccca ccctgccgta ttcggtaatc gctcttacca cccttatatt
      661 caaaccgatt ttgccgaaag tcagctagag ttaatcactc ccccgaatgc taaattagaa
      721 gacagttttc gttggctctc tgctattcac gaagtggtat tgcgttcgtt gccagaggag
      781 gaatatatct tccctctcag tatgccggca ggcttgccgc cggaagagca aattcaagaa
      841 gcacaatttg acaaccctga agatgtgcaa tatcgccaac acctttccaa aaactatggg
      901 aaatataagc aaatggtaag cggcgtgcat tataattttc aactttcaac ggaatttgtc
      961 gaaaaagcct ttgctttaca aacggaatat agcgagctga aagccttcca aaacgcactt
     1021 tatatgaagt tagctaataa ctttttgcgt tatcaatgga ttttgctcta tttattggct
     1081 gccacgccta cggtggaatc gcaatatttt tatgaaaaat caccgcttgc gagagggcaa
     1141 cttgtccgca gcttgcgttc aagcccttat ggttatgtga attcggaaaa agtggtggtt
     1201 aatcacgaca gcttagaaag ctatgttgaa agccttgaag ccaatgttgc caacggatta
     1261 ttaattgccg aaaaagaatt ctattccaat gtgcgtttac gagggggcaa aaaagcccgt
     1321 gagctattgg aaaacggtgt gaaatacgcc gaattccgtt tatttgattt aaaccctttc
     1381 gccccttacg gcattgagct gaatgatgcc aaatttatcc attatttctt actcggtatg
     1441 ctttggctgg aggaaacaag cggtcaaaaa gaggtcgaaa tcggcaaaca gaaactttac
     1501 caagtcgcac ttgaagatcc aagaacagaa accgctttcc gtgccgaagg tgaagccatt
     1561 ttaaatcaac ttttagcgat gcttgaagaa ttatctgccg gtcaggaaag cctagatttg
     1621 gtgcgggaaa aattagctca atttgctgat ccaagcaaaa ccctgaacgg acgtttaatt
     1681 gccgcaatag aggaagtcgg cagttataaa gcattggggg ctaaattggc tcaaacctat
     1741 aaggcacaag cctttgagcg tttttatgcg ttatcggcat ttgataatat ggaactttcc
     1801 actcaagccc tgttttttga tttgatccaa caagggattg aggtggaagt actggacgaa
     1861 aacgatcaat tcctcgccct aaaatttggc gatcatttgg aatatgtgaa aaacggcaat
     1921 atgaccagcc gagatcaata catttcaccg ctgattatgg aaaacaaagt ggtgacgaaa
     1981 aaagtattgg caaaagccgg ctttaacgtg ccgaaaagcg ttgaatttac caaccttgaa
     2041 caggctgtgg cacattatcc gctatttgaa ggcaaagcgg tggtgattaa gccgaaatcg
     2101 actaactatg gtttagggat taccatcttc caacaagcgg tcaaaaatcg agaagatttt
     2161 gcaaaagcgg ttgaaattgc gttccgtgaa gacaaagagg tgatggtgga agactattta
     2221 gtcggtactg aataccgctt ctttgtactg ggcgatgaaa cccttgcggt gttactgcgt
     2281 gtaccggcga atgtagtggg agatggcatt cacagtgtaa aagagctggt ggagcgtaaa
     2341 aatgacgatc cgctacgtgg cgatggctct cgtagcccgt tgaaaaaaat tgccttaggt
     2401 gaaattgagc agttacaact taaagagcaa ggcttaaccg tagattccgt accggcgaaa
     2461 ggtcaaacag ttcaactgcg tgccaattcc aacatcagca ccggcggcga ttcgattgat
     2521 atgaccgacc aaatgcacga aagctataga caaattgcag tgggaattgc tcaggcaatg
     2581 ggggctaaag tgtgtggggt ggatttgatc attcctgatc tcaccaaacc tgccgagcca
     2641 aatttaagct cttggggcgt cattgaggca aacttcaacc caatgatgat gatgcacatt
     2701 ttcccttacc aaggcaaatc ccgccgttta accaaagcgg taatcaaaat gctgtttcct
     2761 gagataggca attaaatttt ctctctatac ct
//
LOCUS       DANLUQ010000207         2725 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.209, whole genome shotgun sequence.
ACCESSION   DANLUQ010000207 DANLUQ010000000
VERSION     DANLUQ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.209"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..179
                     /locus_tag="PXB27_002428"
     CDS             <1..179
                     /locus_tag="PXB27_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347167.1"
                     /translation="FKEKGYDEFLAEKIRLGLEDMQSGNGLSLDESKARTKQLIERKA
                     RELANFEQENIIYG"
     gene            172..459
                     /locus_tag="PXB27_002429"
     CDS             172..459
                     /locus_tag="PXB27_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5347168.1"
                     /translation="MAKTLRISSRAQNDIEEILASVIEYTGFESSGIRLQEDIYQKFE
                     TIAYMPSAAGRLREDGTREAFTRRYRIVYTELENEVWIITIIHSSRLYPRI"
     gene            complement(585..2687)
                     /locus_tag="PXB27_002430"
     CDS             complement(585..2687)
                     /locus_tag="PXB27_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HDL5347169.1"
                     /translation="MKSFKLLPVSAAVLSVLAANAVAQTASTDAVVLDTVTVTDNQGL
                     KVQSNVVTTKKKDESTQTDLRGLLKDEPAISLGGGNGTSQYLYIRGMGQNSIDVKVDN
                     TYSDSQILYHQGRHMLDPALVKIVSVQKGAGSASAGIGQTNGAVIAKTVDALDLLKNS
                     DKNFGAKLGTGFSTNHAHNFNATLYGKGEIFDALVSGNLVRDRDYKGGKDYVNQFGTN
                     RVPYSALDKTSFLAKLGATLGDHRFVLSHSSERNEGQRLVREEFDVAPNGTKVSPYNR
                     LTVERQAPANRRITVQRTNLEWTGKNLGFAQEATANVYQLVQGRWSENETGNGYAGGV
                     DYATKTKIVTHGANVNFDSALHENVLLKYGVNYRHQEAKPHTKFNPLVKNQEKTDTGV
                     YVEAITAPVDKVTLTTGVRYDHFKFKAMDGKERSNGAFSPSVGLIYEPIEHLSLSASH
                     NYATRSPRLYDALTAHGNRKIVSIGSNTKAEKARNTEIGFNYNNGTFSFDGSYFWQDI
                     KDALGGGRDSHNPNNELIMNLGKIKNRGYELNAGYHNGGFTARIGVAHSKPRFYSPTI
                     WEVNRQTGKREEKPLLSTNPEYASAIGRTWTASLSYRFEQPNLEVGVHHRIVEKVKPE
                     DNFFVTGGTLQTNTGTGKDGYNVTDITLNWKPLKDDSVNVNFAVDNVANKKYNAHGQR
                     GQLSARGREFRAGVNYTF"
BASE COUNT          785 a          540 c          512 g          888 t
ORIGIN      
        1 tgtttaaaga gaaaggctat gatgaattcc tagcagaaaa aattcgcctt ggcttagaag
       61 atatgcaatc aggtaatggt ctttcactcg atgaatcaaa agcacgaacc aaacaactga
      121 ttgaacgtaa agcaagagaa ctcgcaaact ttgaacaaga gaatatcatt tatggctaaa
      181 acattacgaa ttagcagtag agctcaaaat gatattgaag aaattttagc gagcgtaatt
      241 gaatatactg gttttgaaag tagtggaatt cgcttacaag aagatattta ccaaaaattt
      301 gagactatcg cttatatgcc ctctgcggct ggacgtttaa gagaagatgg cacaagagaa
      361 gcattcacta ggcgttatcg tattgtttat accgaattag aaaatgaagt gtggattatt
      421 accattattc atagtagcag actttatcca cgcatttaaa taacaaacga ctaaaatagt
      481 cggtttttta tttgcaaaaa attaataaaa tatcaccgct tgtaagctcc ataaaaaaaa
      541 gcccgattgt aacatcgggc gttttagtct gaatttaagt cagattagaa tgtgtagttc
      601 acacctgcac ggaattcacg tccacgagct gataattgac cacgctgacc gtgtgcgttg
      661 tattttttat tcgccacgtt atccactgcg aagtttacat tcacgctatc atctttaagt
      721 ggtttccagt ttaaggtgat atcagttaca ttgtagccgt ctttgcctgt accggtattt
      781 gtttgtaatg ttccgcccgt aacgaagaaa ttatcttcag gtttcacttt ctcaacaata
      841 cggtggtgta cgccaacctc taaatttggt tgttcaaagc gatagcttaa agaagctgtc
      901 caagtacgac cgatagctga ggcgtattct gggttagtac ttaataatgg tttttcttcc
      961 cgcttaccag tttgtctgtt tacttcccaa atagttggtg agtagaaacg cggtttgctg
     1021 tgtgcaacac caatacgagc agtaaaacca ccattatggt aacctgcatt taactcataa
     1081 ccacggtttt taattttacc taggttcata attaactcat tattaggatt atggctatca
     1141 cgaccaccac ctaatgcatc ttttatatct tgccagaaat agctaccatc aaaactgaat
     1201 gtaccattgt tatagttgaa accgatttca gtattacgag ctttctccgc tttagtattg
     1261 ctaccaatac taacgatttt tctatttccg tgagccgtta gtgcatcgta taaacgaggg
     1321 ctacgagttg catagttgtg gcttgcactt aagcttaaat gttcaattgg ctcatagata
     1381 agcccaacgc ttgggctgaa tgcaccatta ctacgctctt taccatccat tgctttgaat
     1441 ttgaagtggt cgtaacgtac accggtagtt aaagttactt tatctactgg agcagtaatt
     1501 gcttcaacat acacgccggt atcggttttc tcttggtttt tcacaagagg gttaaattta
     1561 gtgtgtggtt tagcttcttg gtggcgatag ttcacaccgt attttaataa tacattttcg
     1621 tgaagggcag aatcaaagtt tacgtttgca ccgtgggtta ctattttagt tttggttgca
     1681 taatctacac caccagcata accattacct gtttcattct cagaccaacg accttgtact
     1741 aactgataca cgttagctgt tgcttcttga gcaaaaccta agtttttacc agtccattct
     1801 aagttagttc tttgaaccgt aattctacgg ttagctggag cttggcgttc tacagttaag
     1861 cggttgtacg gagaaacctt tgtgccgtta ggtgctacat caaattcttc acgcactaaa
     1921 cgctgacctt catttcgctc acttgaatgg cttaatacaa aacggtgatc tcccaatgtt
     1981 gcacctaatt tagctaaaaa gctggtttta tctagtgcac tatacggaac acggtttgtg
     2041 ccgaattggt ttacgtaatc tttaccgcct ttatagtcgc gatctctcac aagattacct
     2101 gagactaaag catcaaagat ttcgccttta ccatataagg ttgcattgaa attgtgagcg
     2161 tggtttgtgc tgaagccagt acctaatttt gcaccgaagt ttttatcgct gttttttaat
     2221 aaatctaatg catcaacggt tttagcaatt actgcaccat tagtttgacc aataccggct
     2281 gaagcactgc ctgcaccttt ttgtacagaa acaattttca ccaatgccgg atctaacata
     2341 tgacgacctt ggtgatacaa aatttggcta tctgagtaag tgttatctac ttttacatca
     2401 attgagtttt gacccatacc acgaatatag agatattgtg atgtaccgtt accaccacct
     2461 aagctaattg ccggctcatc ttttaataaa ccacgcaaat cggtttgggt gctttcatct
     2521 tttttcttag ttgttaccac attgctttgt acttttaaac cttgattatc tgttacggta
     2581 acagtatcta atactactgc atctgttgaa gcagtttgag ccactgcatt agcagcaagt
     2641 acgctcaata ccgcagcaga aacagggagc aatttgaagc ttttcatttt atttatattc
     2701 cttaggtaaa ttatttaaga ggatt
//
LOCUS       DANLUQ010000208         2678 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.210, whole genome shotgun sequence.
ACCESSION   DANLUQ010000208 DANLUQ010000000
VERSION     DANLUQ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.210"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            139..579
                     /locus_tag="PXB27_002431"
     CDS             139..579
                     /locus_tag="PXB27_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDL5347170.1"
                     /translation="MTACKNGLGFLVLRLFLAYEFFEAGLSKLNGENWFASIQESFPF
                     PFNVLPADLSWAIASGAEILLPILLTLGLFTRFSALGLMILTAVAWYAVHAGNGYNVC
                     NNGYKMALIYLVMLVPLLLQGAGSLSLDFLLQKKSPTKRWLKFL"
     gene            605..892
                     /locus_tag="PXB27_002432"
     CDS             605..892
                     /locus_tag="PXB27_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347171.1"
                     /translation="MKKATLMALAGALTMATATVNAETKSVKADACMEVKDGKCVKTK
                     ATEGKCGEGKCGATSSEPKATEGKCGEGKCGATAPKAAEGKCGEGKCGASK"
     gene            993..1916
                     /locus_tag="PXB27_002433"
     CDS             993..1916
                     /locus_tag="PXB27_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HDL5347172.1"
                     /translation="MLKFTGAGLGYRRDLADGFLSLTDNNIINFMEVAPENWLKMGGQ
                     ARYNFDKVAEKIPVIIHGLSLSLGGQAPLDMTLLKGIKNMQQQYNSFFFSDHLSYCEC
                     EGHLYDLLPMPFTDEAVHHVANRIKQVQDYLGMQISLENTSYYLHSPTSTMNEVEFLN
                     AIAQEADCGIHLDVNNIYVNGVNHGLLDPFVFIDQVDKSRVNYIHIAGHDEDHGAAED
                     VKMTEGESFNMVKGELRHLPTLLVDTHGEAVQKTVWDLLEYTYEHLPHIPPTLLERDF
                     NFPPFQELISEVQHIMSLQKKYAKTRLKNAA"
     gene            1906..2646
                     /locus_tag="PXB27_002434"
     CDS             1906..2646
                     /locus_tag="PXB27_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding domain-containing protein"
                     /protein_id="HDL5347173.1"
                     /translation="MPLKLVEEHKPLLKEIQAEFANAIRHNDVKLMSGNISPQRLGVY
                     SRLVRNNTLGFIDRCYVQLPQHLSPEEWVAVKEKFIREGKAHSPFFQDIAGEFLNFCR
                     ENGCMPPHLLELMDFENAQLLAEVSMATVPSEFEWDNDTIMQFSGAAELRQYAVNFIR
                     SEFKQFDFEPTNVLIWRDTEFGIYYNRLEELDFFLLSSLQEGANSLNGLLAELSEFTE
                     QNLELKALLRQLWEKWVEAEVIYPVYPE"
BASE COUNT          801 a          531 c          571 g          775 t
ORIGIN      
        1 ctataaaaat aggttataat ttttcacaca ttttttgtaa taaacgtata cttattctgt
       61 ctaataatca catttttagt taggagcatt tttatgtcat cattttcgtc atcattttca
      121 actcagtcag cttgctcaat gacagcttgt aaaaacggct tgggctttct tgtattacgt
      181 ctatttcttg cttatgaatt ttttgaagca gggttatcaa aactaaacgg tgaaaattgg
      241 tttgcctcaa ttcaagagag cttcccattc ccctttaacg tactaccagc cgatcttagc
      301 tgggctattg ctagcggagc ggaaatctta ctgcctattt tactcacttt gggtctgttc
      361 acccgcttca gtgcgttagg attaatgatc ttaacagcgg tagcgtggta tgctgttcac
      421 gctggcaatg gctacaatgt ctgcaacaat ggctataaaa tggcactcat ttatttagtg
      481 atgttagtac cacttttatt acaaggtgct ggctcgctct ctctcgattt tctgttacag
      541 aaaaaatccc caaccaaacg ctggttgaaa ttcctttaat cccttaattt atggagaatt
      601 tttaatgaaa aaagcaactt taatggcatt agctggtgca ttaactatgg caaccgcgac
      661 tgtaaacgct gaaactaaat cagtgaaagc agatgcttgt atggaagtaa aagatggcaa
      721 atgtgtaaaa accaaagcta cagaaggtaa atgtggcgaa ggcaaatgtg gagcaacctc
      781 ttctgagcca aaagcaaccg aaggtaaatg cggcgaaggg aaatgtggag caacggcacc
      841 aaaagcggca gaaggtaaat gcggtgaagg caagtgtgga gcttctaagt aagtagcgaa
      901 ggtgcgtttt ctaaacgcac ctaacctctt ataaacacaa tctacaagcg gttaaattcc
      961 gctgattttt tgcaaattaa aggaacaaga cgatgttaaa atttactggt gccggtctag
     1021 gctatcgccg tgacctagcg gacggatttt tatctctcac agataacaat ataattaatt
     1081 ttatggaagt agccccagaa aactggctga aaatgggcgg tcaggctcgg tataacttcg
     1141 ataaagtcgc tgaaaaaatc cctgttatta ttcacggttt atccctctct ctcggcgggc
     1201 aagcgccttt agatatgacg ctactaaaag ggatcaagaa tatgcagcag cagtataact
     1261 cctttttctt ctctgatcat ttaagctatt gcgaatgtga agggcattta tatgacctgc
     1321 tgccaatgcc gtttaccgat gaagccgtac atcacgtggc aaatcgcatt aaacaagtgc
     1381 aagattattt aggtatgcaa atttcccttg aaaatacgtc ctactatctg cattcaccaa
     1441 ccagtacgat gaatgaggta gagtttctca atgcaattgc ccaagaagcg gattgtggca
     1501 ttcaccttga tgtgaataat atttatgtta atggtgtcaa tcacggcttg cttgatccat
     1561 ttgtatttat tgatcaagtg gataaatctc gtgtcaatta tattcatatt gcaggccacg
     1621 atgaagatca cggtgcggcg gaagacgtta aaatgaccga gggtgaatca tttaatatgg
     1681 taaaaggcga actacgtcat ctgccaactt tactggtcga tacacacgga gaagctgtac
     1741 aaaaaactgt gtgggattta ctagagtaca cctatgaaca cctaccgcat attccaccaa
     1801 cactgttaga acgggatttc aacttcccac cgtttcaaga actgattagt gaagtacagc
     1861 acattatgag cttacagaaa aaatatgcaa aaacgaggct aaaaaatgcc gcttaaactc
     1921 gttgaagaac acaaaccgtt actcaaagag attcaagccg agtttgcgaa tgcaattcgc
     1981 cataacgatg taaagctgat gagtggtaat atctctccac agcggcttgg cgtctattct
     2041 cgcttggttc gcaataatac tctcggtttt atcgaccgtt gttatgtaca gctccctcaa
     2101 catctaagtc ctgaagaatg ggttgccgtt aaagaaaaat ttatacgaga aggtaaggct
     2161 cattcgccct ttttccaaga tattgctggc gaatttctca atttttgccg agaaaatggc
     2221 tgtatgccac cacacttgct tgagttgatg gactttgaaa atgctcaatt actggctgag
     2281 gtatcaatgg caaccgtacc atcagaattt gaatgggata acgatactat aatgcagttt
     2341 tctggtgcag ctgagctgcg tcaatatgcg gtaaacttta tccgtagcga gttcaaacag
     2401 tttgactttg agccgaccaa cgtactgatt tggcgagata cggaatttgg catctattac
     2461 aaccgcttgg aagagctgga tttcttcctg cttagctcgt tgcaagaggg ggcaaattcc
     2521 ctgaatggct tactagcaga actcagcgag tttaccgagc aaaatctgga gctaaaagcc
     2581 ttgttacgcc aactttggga aaaatgggtt gaagcagagg tgatttatcc tgtctatcct
     2641 gaatgaggat aaggtatgtg atatgtactg acatacat
//
LOCUS       DANLUQ010000209         2629 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.211, whole genome shotgun sequence.
ACCESSION   DANLUQ010000209 DANLUQ010000000
VERSION     DANLUQ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.211"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            11..769
                     /locus_tag="PXB27_002435"
     CDS             11..769
                     /locus_tag="PXB27_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL5347174.1"
                     /translation="MIPLERQREILRLINNNGVASINFLAEQLKVSHMTIRRDIQKLE
                     EQGKVSLVSGGVQSLERLIMELPHDDKSMLFQEQKSAIGSLASQLIPSNTTIYLDAGT
                     TTLEIAHRIIDRNDLLVVTNDFVIAHYLSNSGKCDLIHIGGSVCKENLSTTGSLAAEF
                     LKNLSIDMAFISTSSWNLKGLTTPNENKIPVKKAILEASKQNILVTDSSKYGKVATFF
                     VCPLTVFDQIICDKGLFENVRDALKGLSIKLDLG"
     gene            complement(909..2300)
                     /gene="ffh"
                     /locus_tag="PXB27_002436"
     CDS             complement(909..2300)
                     /gene="ffh"
                     /locus_tag="PXB27_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011199698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDL5347175.1"
                     /translation="MFENLSDRLSKTLRNITGKGRLTEDNIKDTLREVRMALLEADVA
                     LPVVRDFINKVKERALGVEVNKSLTPGQEFLKIVQSELETAMGEANEELNLAAQPPAV
                     ILMAGLQGAGKTTSVGKLAKFLKERHKKKVLVVSADVYRPAAIKQLQTLAEALKVDFF
                     PTETSQKPVAIAEAALKHAKLNFFDVLIVDTAGRLHVDGEMMEEIQQIHAVLNPIETL
                     FTVDAMTGQDAANTAKAFNEALPLTGVILTKVDGDARGGAALSIRQITGKPIKFLGVG
                     EKTDALEPFHPDRVASRILGMGDVMSLIEDIQRSVDQEKAEKIAAKFKKGDEFTLEDF
                     REQLIEMKKMGGMMSMLDKLPGAKNLPDHVKSQVDDKMFMKMEAIINSMTLKERANPD
                     IIKGSRRRRIALGSGTQVQDVNKLLKQFDDMQRMMKKMRKGGMAKMMRGVKGMMGGGL
                     GGLGGLGSMFGKR"
BASE COUNT          790 a          552 c          513 g          774 t
ORIGIN      
        1 aggttaaaaa atgatcccct tagagagaca acgagaaata ctacgactta ttaataataa
       61 tggtgtcgca agtattaatt ttctggcaga acagctaaaa gtatcacata tgacaattcg
      121 ccgtgatatt caaaagctgg aagagcaagg taaggtatcc ctagtatctg gtggagttca
      181 atctcttgaa cgattaatca tggaattacc acacgatgat aaatcaatgt tattccaaga
      241 gcagaaatca gctataggat ctttagcatc acagctaatt ccctcaaaca caactattta
      301 tcttgatgct ggaactacta cattagaaat agctcatcgg ataatcgata gaaatgactt
      361 actagttgta acaaacgatt ttgttattgc tcactatctt agtaattcag gaaaatgtga
      421 tttaattcat ataggaggaa gtgtctgtaa agaaaaccta tcgacaactg gttcacttgc
      481 agcagaattt ctgaaaaacc tatcaatcga tatggcattt atatctactt catcctggaa
      541 tttaaaaggt ctaacaacgc cgaacgaaaa taaaatcccc gttaaaaaag caattttaga
      601 ggcatccaag caaaacattt tagtgacaga ctcttcaaaa tatggaaaag tcgcaacttt
      661 ttttgtttgc cctcttactg tatttgacca gattatttgt gataaaggat tatttgagaa
      721 tgtacgagac gcactgaaag ggctaagtat taaattagat ttaggataat cttcttacca
      781 attttttctg agaacgacaa gcaatcaaaa agtattattt ttgcaaataa gatattaata
      841 gggaaagtta acaatcctag caacggattg ttaactgatt ttcggctggt actaataaaa
      901 taggaatatt aacgtttacc aaacatactg cctaagccac cgagaccgcc tagaccacca
      961 cccatcatac ctttaacacc acgcatcatt tttgccattc cgcctttacg catttttttc
     1021 atcatacgtt gcatatcatc aaactgcttg agcagcttat ttacatcttg cacctgtgtg
     1081 ccggagccaa gtgcgatacg gcggcggcgt gagcctttaa taatatccgg atttgcccgc
     1141 tcttttaagg tcatagaatt gatgatggct tccattttca tgaacatttt atcatcaacc
     1201 tgacttttaa cgtggtcagg caggtttttc gcccccggta gtttatcgag catcgacatc
     1261 attccaccca tttttttcat ttcgataagc tgctcacgga aatcttccaa ggtaaattca
     1321 tcgccttttt tgaatttggc ggcaattttt tccgcttttt cttgatcaac cgagcgttga
     1381 atatcttcaa ttaaagacat tacatcgccc atacctaaaa tgcgggaagc cacacggtca
     1441 gggtggaacg gctcaagggc atcagttttt tcacccacac ctaagaattt aatcggttta
     1501 ccggtgattt ggcgaataga aagtgccgca ccgcctcggg catcgccatc gactttggta
     1561 agaataacac cggtaagcgg caacgcttcg ttaaaggctt ttgcggtgtt ggcggcgtct
     1621 tgaccggtca tcgcatcaac cgtaaatagc gtttcaatcg gattcagcac agcgtggatt
     1681 tgttggatct cttccatcat ttcgccatca acgtgtaaac gaccggcagt atccacaatc
     1741 aatacatcaa agaaattaag cttagcgtgc ttcagtgccg cttctgcaat cgctaccggt
     1801 ttttggctgg tttcggtcgg gaagaagtcc acttttaacg cttcagcaag ggtctgtagc
     1861 tgcttgatag cggcagggcg gtaaacatcg gcagaaacta ccaacacctt tttcttgtgg
     1921 cgttctttca ggaattttgc caatttgccg acagaggtgg ttttacccgc accttgcaaa
     1981 cctgccatta aaattaccgc aggcggttgg gccgcaagat tcagctcctc gttggcttct
     2041 cccattgccg tttctaattc gctttgtacg atttttaaga actcttgccc cggcgtgagg
     2101 cttttgttta cctcaacccc taacgcacgc tctttgacct tgttgatgaa atcacgcaca
     2161 acagggaggg caacatccgc ctccaacaat gccatacgaa cttcacgcag ggtatcttta
     2221 atattatctt ccgttaggcg acctttgccc gtaatgttac gcagcgtttt agacagtctg
     2281 tcagagaggt tttcaaacat atcagctttc tcaatttgca aaattttagg aaaaaagtac
     2341 cgcttgtgag cggaaagttg ccgccattat acaaaaaaaa gcgttacggt tatagggaaa
     2401 taaaaaaacg gcatagcgat taaactatgc cgtaaaattt gacgatattc gaggaatcat
     2461 tatttagtta tattgtgcga tcataatact ccgaattgcg tataaaagtg aagtgatatt
     2521 ttcaaattta tgttagaaat gagaggtaaa ttacaaaatt aaaggggata ttattgtttg
     2581 aaaaaataag agcgaaaaga aaaccctgat gattgctcat cagggcttc
//
LOCUS       DANLUQ010000210         2602 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.212, whole genome shotgun sequence.
ACCESSION   DANLUQ010000210 DANLUQ010000000
VERSION     DANLUQ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.212"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            282..2255
                     /locus_tag="PXB27_002437"
     CDS             282..2255
                     /locus_tag="PXB27_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HDL5347176.1"
                     /translation="MHSILKKILGKSKSSSQSSVKSDNVFAIEQDSEGWLYPKNASQL
                     LNTELRQKYLGLLWQQVSMNRQMFEVLYQQPIERYAEMVQLLPASESHHHSHIGGMLD
                     HGLEVIAFSAKVRQNYILPPNVAPEEQSKQRDAWTAAAIYLALVHDIGKVVVDIEITL
                     KDGSRWFAWNGQPPQPYKFKYIKGRDYHLHPVLGGFLANQLIPKSAFDWLGQYPEVIS
                     SLMYAMSGHNDKAGLLSEIVQKADQHSVTLAIGGDVNKLVQRPVNSLPKQIVLALRYL
                     LEQKIKLNTPKGPADGWFTEDGLWLVSKTAADNIRTYLLGQGISVPKDNPKLFDEMQS
                     LGIVEESPSGGAIWSCRIQADSGWTPATTFTLLRVKPEVAWENINDRPAYFAGKVKVI
                     EQITEEVNAEVQPPLVEETTKISLLPSKQSEAIIPSEETEISKAITSSDNATSAEVII
                     NNENDLTSFVLDMFAPTETIEQNISVISSNKEQQIVMTTLTEQEKVPPKKVKLEKSKK
                     KNLQLTTLEPWNDMINPITGKEFVNWLKEGIATNKFAINKPSAKLHIVDGNLFLVTPV
                     IFEMYLQECGQPYDKETINNLQYDFQGLGLHSLKKTMTNGKPDTINFWKCDVIGPRKT
                     SSLTGYLIKNTHLLFGDKMLLNNHCLTLQEEDT"
     gene            2295..>2602
                     /locus_tag="PXB27_002438"
     CDS             2295..>2602
                     /locus_tag="PXB27_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase SAM-like domain-containing
                     protein"
                     /protein_id="HDL5347177.1"
                     /translation="MFFYRHYLRADTKRSYNNIIRIIKKRYPNLNANDVTTDMLIEWR
                     NQVVGNTIKAVTWNNYVRHLKALYNFAIAQGLLCMSSNPFNHLFIRESRRKKKTYSSE
                     "
BASE COUNT          861 a          476 c          523 g          742 t
ORIGIN      
        1 ttgtaatctc gcaattcaac atcactaata aaaaattctc caacatattc agagagtttt
       61 ttctactgat accaactaaa ttttggttca atactcgaag ttaaatttgg ttcagagaaa
      121 aaagcctcta aatggcaaaa gggtaagggg ccctaacggg caagggtaat cccctaaggg
      181 ggataagagt aacctttcca aggggaaagg tcttacccaa aagggcaaag ggctaaacgg
      241 ctttctttca acgcaaacat catctttttc ggtctatttc aatgcactca attctcaaaa
      301 aaattcttgg caaaagtaaa tcctcgtctc aaagttcggt taaatctgac aatgttttcg
      361 ctattgagca ggatagtgag ggctggttat atccgaaaaa tgcctcccaa ctacttaata
      421 cggaattacg ccaaaaatat ctgggtctac tttggcaaca ggtatcaatg aaccgtcaaa
      481 tgtttgaggt actttatcag cagccaatag aacgttacgc tgagatggtt caactattac
      541 cagcttctga atcacatcat cattcacata tcggcggaat gctcgatcac ggtttggaag
      601 ttattgcttt ttcagccaaa gtccgtcaaa actatatatt gccaccgaat gtcgcacctg
      661 aagaacaatc taagcaacga gacgcttgga ctgccgctgc aatttatctt gccttggttc
      721 atgatattgg caaagttgtg gttgatattg aaatcactct taaagacggc tctcgttggt
      781 ttgcttggaa tggacagcca cctcaacctt ataaattcaa atacatcaaa ggtcgagact
      841 accatcttca tccggtgcta ggaggattcc ttgcaaatca gctcattccc aagtctgcgt
      901 ttgattggct gggacagtat ccagaagtca tttcttcact gatgtatgca atgtctgggc
      961 ataacgataa agccgggctt ttatctgaga tcgtgcagaa agccgatcag catagtgtta
     1021 cgctagcaat cggtggcgat gtgaataagc tggtacagcg accagttaat tcattgccga
     1081 agcaaattgt gctggctcta cgttatttac tggagcagaa aatcaaatta aatacgccaa
     1141 aaggaccagc agacggctgg tttaccgaag atgggctgtg gctggtaagt aaaactgctg
     1201 ccgataatat ccgaacttat ttactgggac aggggatttc cgtgcctaaa gataatccaa
     1261 aactgtttga tgaaatgcaa tctcttggta ttgtggaaga gtctccttcc ggcggtgcaa
     1321 tttggagctg ccgtatccaa gccgattccg gttggacacc ggcgacaaca tttacgctat
     1381 tacgagttaa accggaggtt gcttgggaga atatcaacga tagaccggcg tattttgccg
     1441 gaaaagtcaa agtgattgaa cagataactg aagaggtcaa tgcagaggtt cagcctccat
     1501 tagttgaaga aaccactaaa atttctctat taccgagcaa gcaatcggag gctattattc
     1561 cgtctgagga aacggaaata tcgaaggcaa ttacaagttc agacaatgca acatctgcgg
     1621 aagtaatcat aaataatgaa aatgatttaa cttcttttgt attggatatg tttgctccta
     1681 cagagacaat tgagcaaaat atttctgtca tttcttcaaa taaagagcag caaatcgtaa
     1741 tgacaacact tacggaacaa gaaaaagttc cgccgaaaaa agttaaatta gagaaaagta
     1801 agaaaaaaaa tcttcaatta accactctag aaccatggaa tgatatgatt aatccaatta
     1861 ctggaaaaga attcgtaaat tggttgaagg aggggatagc aaccaataaa tttgcaatta
     1921 acaaaccttc tgcaaaatta catatagtag atggtaattt atttttagtc acaccagtta
     1981 tttttgaaat gtatttgcaa gaatgtggcc aaccttatga taaggaaact ataaacaatc
     2041 ttcagtatga tttccagggg cttggtcttc attctcttaa aaaaaccatg actaatggta
     2101 agccagatac aataaatttt tggaagtgtg acgtgattgg tcctagaaag acttcctctt
     2161 taacaggata tttgataaaa aatactcatc tcttatttgg ggataaaatg ttactaaata
     2221 accactgttt aactttacag gaagaggaca cataatatgg attctaagat ttccttcgat
     2281 atattattgg aacgattttt ttctatcgtc attatttacg ggcggacacg aaacgaagtt
     2341 ataacaatat tattcgaatt atcaaaaagc gttatccaaa cttaaatgcg aatgatgtga
     2401 ctactgatat gttaattgaa tggcgtaatc aagttgttgg taatacaatt aaagctgtga
     2461 cgtggaataa ttatgtgaga cacctaaaag cattatataa ttttgcgata gcacaggggc
     2521 tactttgcat gagtagtaat ccatttaatc atttatttat tagagaatct agaagaaaaa
     2581 agaaaactta ttccagtgag ca
//
LOCUS       DANLUQ010000211         2594 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.213, whole genome shotgun sequence.
ACCESSION   DANLUQ010000211 DANLUQ010000000
VERSION     DANLUQ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.213"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(424..597)
                     /locus_tag="PXB27_002439"
     CDS             complement(424..597)
                     /locus_tag="PXB27_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347178.1"
                     /translation="MYPPAAYLVPCGRTEFSGRTYGDTVEYLVKVMGERDLCANQIDR
                     IREWQAQTKQGFK"
     gene            complement(602..952)
                     /locus_tag="PXB27_002440"
     CDS             complement(602..952)
                     /locus_tag="PXB27_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HDL5347179.1"
                     /translation="MLNWISGTIAIVILGLCVWLWGQSQMIDSLRAENRAQAQTIEQQ
                     QKANQRLTDSLEQERQAVEKIQKIANELRNKVEMAKNEITSILAQDSCAKADLPNGVA
                     DSIKRLHQQNNHKH"
     gene            complement(925..1494)
                     /locus_tag="PXB27_002441"
     CDS             complement(925..1494)
                     /locus_tag="PXB27_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HDL5347180.1"
                     /translation="MSKSLKYGSGIVCGIAAIITLVQYQHPEIRTNQAGLEIIGNAEG
                     CRRDPYKCPADVITVGIGSTEFGGEKIDPNRIYSDKEIAERWAKDLKIAESCVNRHFN
                     GKDMNDNQFSGMTSAVFNMGCYNMRFYRNKQGQYVQTTIHKLAVNKQFEEMCHRLPDF
                     IRASGKVLNGLVIRREKEKALCLTGLVAR"
     gene            complement(1487..1732)
                     /locus_tag="PXB27_002442"
     CDS             complement(1487..1732)
                     /locus_tag="PXB27_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347181.1"
                     /translation="MKLMKDMGTQSYIWSGFSTWLAWLGEQQNLMLLSLAIGIVTAIA
                     GLIQRFDEWRRRKREDERAEELHQAKMARLRKGLSDE"
     gene            complement(1875..2045)
                     /locus_tag="PXB27_002443"
     CDS             complement(1875..2045)
                     /locus_tag="PXB27_002443"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347182.1"
                     /translation="MTEQLNTQSKSGEEDQEKSQDRYAVGWPSRRISDCVQHFRSRKI
                     YPFASNNDVKQG"
     gene            complement(2071..2256)
                     /locus_tag="PXB27_002444"
     CDS             complement(2071..2256)
                     /locus_tag="PXB27_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347183.1"
                     /translation="MYNLDDAIQECREKFGEDKPYFSEAGTLTFAGCKALSRLRDEKL
                     GIKAPPNFPFPRVEDLS"
     gene            complement(2273..2348)
                     /locus_tag="PXB27_002445"
     tRNA            complement(2273..2348)
                     /locus_tag="PXB27_002445"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2313..2315),aa:Trp,seq:cca)
BASE COUNT          729 a          623 c          443 g          799 t
ORIGIN      
        1 gacgtggctt taacctcgcc ttttttagtc attgggttaa attcctttac ttcattgcat
       61 ttaatagatt agcaatagca ttaatcagct ttggcgacac gaacgccaaa atagggactg
      121 ttactgctag gcttatttgc cataaaccat attccataag aatctccttg atggttacgg
      181 ttaaaaatgt aataatttcc ataacttgtt actcattccg tttcaatggg taataaaaaa
      241 acctcgaaca ccgcaaatgt tcggggtttt gttttatata aatcacatca cttaggtatt
      301 tcctcggtgt tttatccaac caaccccgaa accatagcta aattatgtaa tatttacttt
      361 ccatcaaaac aaaaagggag ctgttacgct cccattttca atagcggtta attagataat
      421 tagctattta aacccttgtt tggtttgtgc ttgccattct ctgatacggt caatttgatt
      481 agcacacaaa tcacgctctc ccatcacttt gacgagatac tcaaccgtat cgccataggt
      541 tctaccgcta aattctgttc gcccacacgg cacaaggtaa gctgccggcg gatacaaata
      601 ctcagtgctt gtgattgttc tgctggtgca gccgcttaat gctatcagca acgccattag
      661 gcaaatcagc tttagcacaa ctgtcttgtg ccagtattga tgtaatttca ttcttggcca
      721 tctccacttt attccgcagc tcatttgcaa ttttttggat tttttcgacc gcttgtcgct
      781 cttgctctaa gctatcggtt agccgttgat tggctttttg ctgctgctca atggtttggg
      841 cttgtgcgcg gttctcagct cttaagctat ctatcatctg tgactgaccc cataaccata
      901 cacacaagcc caaaatcaca atggctatcg tgccactaat ccagttaagc ataatgcttt
      961 ctccttttca cggcggataa ccaatccgtt caatacctta ccactggcac gaataaagtc
     1021 aggtagtcga tgacacatct cctcaaattg tttattcact gcgagtttat ggatggtagt
     1081 ctgcacatat tgaccttgtt tattccgata aaatctcatg ttataacaac ccatattaaa
     1141 tacagcggac gtcattcccg aaaattgatt atcgttcatg tctttaccat taaaatgacg
     1201 attgacacaa ctctccgcaa tcttcaaatc ttttgcccaa cgctcagcaa tttctttgtc
     1261 agaataaata cgatttgggt caatcttttc accgccaaac tccgttgagc caatgccaac
     1321 cgtgattaca tcggcagggc atttatacgg atcacgtctg caaccctcag cattaccgat
     1381 aatctctaat ccagcttggt tagtgcggat ttcgggatgt tgatattgga ctaatgtaat
     1441 aatggcagcg ataccacaaa cgataccact gccatattta aggcttttac tcatcactta
     1501 acccctttct caatcttgcc attttcgctt gatgaagctc ttccgctcgc tcatcttcac
     1561 gcttacgcct acgccattca tcaaatcgct gaataagccc tgcaatggca gtaacaatac
     1621 cgatagcaag actaagtaac attaaattct gctgttcgcc gagccacgcc agccaagtgc
     1681 taaatcccga ccaaatatag ctctgcgttc ccatatcttt cattaacttc atactccacc
     1741 ccgtttcgag gcaataaaaa agcccagtcg tgagactgag ctttggttaa aaattctgct
     1801 agaatactgt tccccaacaa ataaactagc agagggtaaa aatgattgaa tttactctgt
     1861 accaccagaa gcatttaacc ttgcttcacg tcattattac tggcaaatgg ataaattttc
     1921 ctacttcgga agtgctgaac gcaatctgat atacgtcgag atggccagcc cacggcatag
     1981 cgatcttgag atttttcttg atcttcttca ccgctttttg actgagtatt tagttgttcc
     2041 gtcatagcaa tccctcattg atttttgtag ctaagataga tcttcaaccc ttgggaaagg
     2101 gaaatttggc ggagccttaa ttcctaactt ttcatctctt aatcgagaga gggctttgca
     2161 accagcaaaa gtaagtgttc cagcttcaga aaaataaggc ttatcttcac caaatttttc
     2221 tctgcattct tgaattgcat catcaaggtt atacataaca tttactcgca tttggaaagg
     2281 gtggttggac tcaaaccaac aaccaacgat tttggagacc gctgctctac caactgagct
     2341 acacccttaa aatccgtaaa caaaaaagcc ccaagcattt ctgctcaggg ctgttaaact
     2401 aattcggtcg gcatactgcc acgcaaacgc tgaaataact atactcgacg tcgtttctag
     2461 ttattctcct ccgaactgaa tattacttca ggctgcgtct ttaactaaca ttggtaaaca
     2521 tcatttatac tacgtccacc attagcacaa aatatacact tgttacgcac taaaatcaag
     2581 gggaaaattg atat
//
LOCUS       DANLUQ010000212         2545 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.214, whole genome shotgun sequence.
ACCESSION   DANLUQ010000212 DANLUQ010000000
VERSION     DANLUQ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.214"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            107..397
                     /locus_tag="PXB27_002446"
     CDS             107..397
                     /locus_tag="PXB27_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDL5347184.1"
                     /translation="MALRPLHDKVILKREEVETKSAGGIVLTGSAATKSTRGKVIAVG
                     TGRILDNGSVQPLAVKVGDVVIFNEGYGVKTEKIDGEEVLILAEHDILAIVE"
     gene            466..2106
                     /gene="groL"
                     /locus_tag="PXB27_002447"
     CDS             466..2106
                     /gene="groL"
                     /locus_tag="PXB27_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011608625.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDL5347185.1"
                     /translation="MAAKDVKFGNDARVKMLKGVNILADAVKVTLGPKGRNVVLDRAY
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIVNE
                     GLKAVAAGMNPMDLKRGIDKAVAAVVEELKAISKPCETSKEIEQVGTISANSDSTVGQ
                     LIAQAMEKVGKEGVITVEDGTGLEDALDVVEGMQFDRGYLSPYFINKPEAGTVELENP
                     YILLVDKKVSNIRELLPVLEGVAKAGKPLLIIAEDIEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIAILTAGTVISEEIGMELEKATLEELGQAKRVVISKDNTTIID
                     GIGDETQIKGRVAQIRQQIEDSTSDYDKEKLQERVAKLAGGVAVIKVGAATEVAMKEK
                     KDRVDDALHATRAAVEEGIVPGGGVALVRAANKVADTLKGDNEEQNVGIKLALRAMEA
                     PLRQIVANAGEEASVVARNVKEGSGNYGYNAGTEQYGDMLEMGILDPTKVTRSALQFA
                     ASIAGLMITTECMITDLPKEEKLDPAAMGGMGGMGGMM"
     gene            complement(2231..2545)
                     /locus_tag="PXB27_002448"
     CDS             complement(2231..2545)
                     /locus_tag="PXB27_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010945564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5339 domain-containing protein"
                     /protein_id="HDL5347186.1"
                     /translation="MLGITLCTILVSSNAIASVKTSSLTVAKPTLSKNLPTECEKMFN
                     TANKLVSDAERQPGTHTQVLKMKNKLSLTKQQILKMDSELQQKSCEKGLTALNSLKQK
                     HQ"
BASE COUNT          803 a          417 c          615 g          710 t
ORIGIN      
        1 atttgaccgc ttgcacttga aactctaaaa tctgccttta tattgttagg cgttacgcat
       61 tttgcgtgaa gttttattta ttcattttta agaaaaggaa catattatgg cacttcgtcc
      121 attacacgat aaagttattt taaaacgtga agaagttgaa acaaaatcgg caggcggtat
      181 tgttttaacc ggttcggcag caacaaaatc aacccgtggc aaagtaattg cagtaggaac
      241 aggtcgcatt ttagacaatg gttctgttca gcctttagca gttaaagtgg gcgatgtagt
      301 gatttttaac gaaggctacg gcgtaaaaac cgagaaaatt gacggcgaag aagtgctaat
      361 tttagcagag cacgatattt tggcgattgt agaataattt cgttctgcac aagcggttaa
      421 aaaagcaaaa ttttttacat atttcaaata caaaggattt agaaaatggc agcaaaagac
      481 gtgaaatttg gtaacgatgc acgcgtaaaa atgttaaaag gcgtgaatat tttagccgat
      541 gcggtaaaag taactttagg tccgaaaggt cgtaatgtgg tgttagatcg tgcttatggt
      601 gcaccaacga ttacaaaaga cggtgtgtcg gttgcccgtg aaatcgaatt agaagataaa
      661 tttgaaaata tgggggctca aatggtgaaa gaggttgcct ctaaagccaa tgatgccgcc
      721 ggtgacggta caactaccgc aactgtatta gctcaagcga ttgtaaacga gggcttaaaa
      781 gcagtggctg cgggtatgaa cccaatggat ttaaaacgtg gtatcgataa agcggttgca
      841 gcagtggttg aagagttaaa agcgatttca aaaccgtgcg aaacctcaaa agaaattgag
      901 caagtaggga cgatttctgc aaactcagat tcaacagtcg gtcaattaat tgctcaagca
      961 atggaaaaag tgggcaaaga gggcgtgatt actgttgaag acggtacagg tttagaagat
     1021 gcattagacg tggttgaggg tatgcaattt gaccgtggtt acttatcgcc atactttatc
     1081 aataaaccgg aagccggtac ggttgagtta gaaaacccat atattctttt agtggataaa
     1141 aaagtgtcta acattcgtga attattaccg gtgttagaag gtgttgcaaa agcaggtaaa
     1201 ccgttattaa ttattgccga agatattgaa ggcgaggcgt tagctacctt agtggtgaac
     1261 actatgcgtg gtatcgtgaa agtggcggca gtgaaagcac ctggctttgg tgatcgccgt
     1321 aaagcaatgt tacaagatat tgcgatctta actgcaggta ctgtgatttc tgaagagatc
     1381 ggtatggagc ttgaaaaagc aacccttgaa gagcttggtc aagcaaaacg tgtggttatc
     1441 agcaaagata acaccacaat tattgacggt attggtgatg aaactcaaat caaaggtcgt
     1501 gtagcacaaa tccgccaaca aatcgaagat tcaacttcag attacgacaa agaaaaacta
     1561 caagagcgtg tagcaaaatt agccggtggt gttgcggtaa ttaaagtagg tgcggcaact
     1621 gaagtggcta tgaaagagaa aaaagaccgt gttgatgatg ctcttcacgc aacccgtgct
     1681 gcggtggaag aaggtattgt accggggggc ggagttgctt tagtgcgtgc agccaacaaa
     1741 gtggctgata cactaaaagg cgataacgaa gagcaaaatg taggtatcaa acttgccttg
     1801 cgtgcaatgg aggctccatt acgccaaatc gttgccaatg caggtgaaga agcctcagtt
     1861 gtagcccgca acgtgaaaga aggttcaggc aactacggct ataacgcagg tacagagcag
     1921 tacggcgata tgcttgaaat gggaatttta gacccaacta aagtgactcg ttcagcactt
     1981 caattcgcgg cttctattgc aggtttaatg atcactacag aatgtatgat taccgatctg
     2041 ccaaaagaag agaaattaga tcctgccgca atgggcggta tggggggaat gggcggaatg
     2101 atgtaattgc ccattgagtg aacaaaagct actgaaatta attcagtagc ttttttgttg
     2161 gtaacaagtg gttaaattta tgctattttt tgcaaataaa aaaccaagca agtaagcttg
     2221 gttttagcgt ttattgatgt ttttgtttaa ggctgtttag tgctgttaaa cctttctcac
     2281 aacttttttg ctgtaattca gaatccattt ttaggatttg ttgctttgtt aaagaaagtt
     2341 tatttttcat ttttagcact tgagtatgag taccaggctg tctttctgca tcagagacca
     2401 atttatttgc cgtgttaaac attttctcac attcagttgg caaattttta cttaaagtag
     2461 gttttgcaac tgtaagtgat gaggttttaa cacttgctat agcattactg gaaaccaata
     2521 tggtacaaag tgttattcct aaaat
//
LOCUS       DANLUQ010000213         2524 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.215, whole genome shotgun sequence.
ACCESSION   DANLUQ010000213 DANLUQ010000000
VERSION     DANLUQ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.215"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..1642
                     /locus_tag="PXB27_002449"
     CDS             <1..1642
                     /locus_tag="PXB27_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDL5347187.1"
                     /translation="DLNADTSQAFLNELDQTLWTAADKLRKNLDAANYKHIVLGFIFL
                     KYISDSFTDFQAKLKTQLTTPESELYLDPALFDEQEFSQILAEELEQRDYYAAENIFW
                     VPEQARWDNIKSLSKLNLGDELPWGDKFKGVSRLIDDAFEAIERENPKLKGVLQRIAG
                     FGVPDEMLTGLIDLFSRTNFTQPMHNGEPVHLQAKDILGHVYEYFLGQFALAEGKKGG
                     QYFTPKSIVTLIVEMLEPYSGRIYDPAMGSGGFFVQADRFIQAHAGNRNAISVYGQES
                     NSTTRKLAVMNMAIRGIPFDFGDKPEDTLLNPLHIDKKMDVVMANPPFNQKEWWNESL
                     ANDPRWAYGTPPQGNANFAWLQHMIYHLSPKGKMALLLANGSMSSQTSGEGDIRKNIV
                     QADLVEAMIALPNQLFTNTQIPACIWIINKAKARKGEVLFINATQIGYLKDRVLRDFT
                     ADDIAKISDTYHNWQKQNGYENIPAFCYCATLDEIAKNDFVLTAGRYVGAVQEENDGV
                     PFAEKMQELTALLNEQFKQGRELEQQIAENLKGLGYGI"
     gene            1632..>2524
                     /locus_tag="PXB27_002450"
     CDS             1632..>2524
                     /locus_tag="PXB27_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL5347188.1"
                     /translation="MAFNQYVFSDIVELISEKIKIKDLKKENYISTDNMLPNFGGITL
                     AENLPNSASCNRFAKKDILFSNIRTYFKKVWLAEFSGGCSPDVLVMRSKNTDILLNEY
                     LFLLIRSDDFINFTVISANGAKMPRGDKNAMKGFIFNIPSIEYQKKCIANYFAFDQKI
                     QLNTQTNQTLEAIAQAIFKSWFVDFDPVRAKAAALSEGKSEHEANLAAMSVICGKDTS
                     ELNDTEYKALWQIAEAFPSEFGDEGLPIGWKFNQADNLFDVGIGKTPPRKESEWFSDN
                     ANDTEWISIKDMGNQGLFITE"
BASE COUNT          795 a          590 c          521 g          618 t
ORIGIN      
        1 tgatctcaac gctgacacaa gtcaagcctt tttaaacgaa ctcgaccaaa ccctctggac
       61 tgccgccgac aaactgcgta aaaacctcga tgccgccaac tacaaacaca tcgttcttgg
      121 ctttatcttc ctaaaataca tctccgacag ctttaccgat ttccaagcca agctaaaaac
      181 ccagcttacc acccccgaaa gcgaactcta tcttgaccct gcactatttg acgaacaaga
      241 atttagccaa attcttgccg aagagttgga acagagagat tactacgccg ctgaaaacat
      301 cttttgggtg ccggagcaag cccgctggga caacatcaaa tcattaagca aactcaatct
      361 tggcgatgaa ttgccttggg gagacaaatt taaaggtgtc agccgcttga ttgatgatgc
      421 ctttgaagcc atcgaacggg aaaaccccaa actcaaaggc gtactccaac gcattgccgg
      481 ctttggcgtg cctgatgaaa tgctcacagg cttaattgac ctgttctcac gcaccaattt
      541 cacccagcct atgcacaatg gcgaacctgt gcatctgcaa gccaaagaca ttttagggca
      601 cgtctatgaa tactttcttg ggcaatttgc ccttgccgaa ggcaaaaaag gcggtcaata
      661 cttcacgcca aaatccatcg ttaccctgat tgttgaaatg ctcgaaccct attcagggcg
      721 gatttacgac ccagctatgg gcagcggcgg cttttttgtg caagctgacc gctttattca
      781 ggctcacgca ggcaaccgca acgccatttc cgtttatggg caagaatcca actccaccac
      841 tcgcaaactg gcggtgatga atatggcgat tcgtggtatt ccctttgact ttggcgacaa
      901 gcccgaagat accctactaa accctttgca catcgacaaa aaaatggatg ttgtgatggc
      961 aaatccgccc tttaaccaaa aagagtggtg gaatgaaagc ctagcaaacg atccacgctg
     1021 ggcatacggc acaccgccgc aaggcaacgc caactttgcg tggttgcaac atatgattta
     1081 ccacctctcc cccaaaggca aaatggcact cctgctcgcc aacggctcaa tgagcagcca
     1141 aacttcaggc gaaggcgaca ttcgcaaaaa catcgtgcaa gctgaccttg tcgaagcgat
     1201 gattgccctg cccaatcagc tattcaccaa cacccaaatc cctgcctgca tttggattat
     1261 caataaagcc aaagccagaa aaggtgaagt gctgtttatc aacgccaccc aaataggcta
     1321 cctgaaggac cgcgtcttgc gtgattttac cgctgatgac atcgccaaaa tcagcgacac
     1381 ctaccacaac tggcaaaaac agaacggcta cgaaaatatc cctgcgtttt gttattgtgc
     1441 cacgctggac gaaatcgcca aaaacgattt tgtgctgacc gcagggcgat atgtcggtgc
     1501 ggtacaagaa gaaaatgacg gcgtgccgtt tgcagaaaaa atgcaggaat tgaccgcttt
     1561 attgaatgaa caatttaaac aagggcggga attggaacag caaatcgcag agaatttaaa
     1621 ggggttggga tatggcattt aatcagtatg tattttcaga tattgttgaa ttaatatccg
     1681 aaaaaataaa aatcaaagac ttaaaaaaag aaaactatat ttcgacagat aatatgctgc
     1741 ctaattttgg tggaataaca cttgctgaaa accttccaaa ttcagcttct tgcaataggt
     1801 ttgccaaaaa agacattttg ttttccaata taagaaccta ctttaaaaaa gtttggcttg
     1861 ctgaattttc aggtggttgt tctcctgatg ttctggtaat gcgcagtaaa aacacagata
     1921 ttttattaaa tgagtattta tttttactta tccgttctga tgattttatt aactttacag
     1981 ttatatcagc aaatggagca aaaatgccac gaggggataa aaatgcaatg aaaggtttta
     2041 ttttcaatat cccaagtatt gaatatcaaa aaaaatgtat tgctaattat tttgcctttg
     2101 accaaaaaat ccaactcaac acccaaacca accaaaccct agaagccatt gcacaggcaa
     2161 tcttcaaaag ctggtttgtg gattttgacc ctgtgcgcgc caaagccgcc gccctaagcg
     2221 aaggcaaaag cgaacacgaa gccaaccttg cggcaatgtc ggtgatttgt ggtaaagaca
     2281 ccagcgagtt aaacgacacc gaatacaaag cgctttggca aatcgccgaa gccttcccaa
     2341 gtgagtttgg agatgaaggg ttacctattg gctggaaatt caatcaagca gacaatttat
     2401 ttgatgttgg tattgggaaa accccaccaa gaaaagagag tgaatggttt tctgataatg
     2461 caaatgatac agaatggatt tctattaaag atatgggaaa tcagggatta tttatcacag
     2521 aaag
//
LOCUS       DANLUQ010000214         2470 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.216, whole genome shotgun sequence.
ACCESSION   DANLUQ010000214 DANLUQ010000000
VERSION     DANLUQ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.216"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            428..806
                     /gene="rnpB"
                     /locus_tag="PXB27_002451"
     ncRNA           428..806
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PXB27_002451"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            867..1505
                     /locus_tag="PXB27_002452"
     CDS             867..1505
                     /locus_tag="PXB27_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1007 family protein"
                     /protein_id="HDL5347189.1"
                     /translation="MKFIKTIITLIALYSMPVFSHPHSFVDLKTNVIVEGSMLKSFQM
                     EWMLDEIASSELIYEVKNSQDKEKTQQNITAEMVQTALQNHYFSTLYDSQNQPIKFTT
                     KPTATRFEIKNNRVIFYITFHLAKPHELKQSKVRFYTYEPSYYIAMEYNRPADVNTSN
                     ASCKATLVQPQVDSKLRLYASGLDKNQSLDMPENGDYSLGAQFAQKVEIICD"
     gene            1496..>2470
                     /locus_tag="PXB27_002453"
     CDS             1496..>2470
                     /locus_tag="PXB27_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt transporter"
                     /protein_id="HDL5347190.1"
                     /translation="MRLKATYWGMIVIGFLSLFALYQLYPMILFKVMEWQKNFNLQLS
                     ASLNELAENQSKAGITLVFISFLYGVFHAVGPGHGKFILSSYLSFEKTKLNQAMKITF
                     ASALVQGLVAISLVTVIVVIFTLSRQYFNLTLKWVERGSFGLMILFGIYWCYQVYRTV
                     NRARKPIIKSIRISQKKAGKLPLVTQKGHIHTENCSCRHKHLPTSNEMQQVRDWKAQL
                     MLVLSIGSRPCSGAILVLFLSYTLNLYAWGVISALAMAVGTGLTLSLFAYLVIIARNK
                     AVKVSQWYFSAQINRNIVWILKFTLGLALILFGMVLFHSSFIDAGTGVF"
BASE COUNT          769 a          392 c          540 g          769 t
ORIGIN      
        1 ggcgtaaacg gaaaagtcag tagagcggaa gcagataagc ggttaaggga ttgtttgatt
       61 gcgcaaaata gacctatttt tgcgtggagc acttggttgg cggtaagatg ttttggatgg
      121 tatttttgga atgagaaaaa aggaaaatag cttgttttat aatctgatta aaatttgagt
      181 ttcggaaaaa tgtatatagt ttcggaaata gaaaggaaat tgattaaata accctttgaa
      241 attattgtaa taaaatagag aaaccaaaat tagttcactt agacttcaaa cgtgcgtaat
      301 ttttacgagt gaattaaagc gaagaggatt aagtttttaa atgaacttaa tcctttttta
      361 ttttgccata ataactacaa gcggttattt ccttgcaatt ttttgcgaaa gcattatact
      421 ctagtgctga gttggttagg caatcgctgg tttattgaag cctctaaatt cgtgaaaacg
      481 taaactgggg gacaagtaaa cgagaggaaa gtccgagcta cacagggcag agtgccggat
      541 aacgtccggg gggcgtgagc ctacgaccag tgcaacagag agcagaccgc cggctttgtc
      601 ggtaagggtg aaagggtgcg gtaagagcgc accgtgtggg tggtaacatt ccacagcagg
      661 gtaaactcca ctcgtagcaa gaccaaatag gaactcaatc ggtggcccgc tgagagttcg
      721 ggtaggttgc ttgagcgtat gtgtaaatgt acgtctagag gaatgattgt ccacgacaga
      781 actcggctta tcgaccaact cactaaattc ttgaaatgcg aacaggggga acttgttcgc
      841 ttttttattt tatagtgaaa gtaaatatga aatttattaa gacgattatc acgttaattg
      901 cattatattc aatgccagtt ttttctcacc cacattcatt tgttgatttg aaaacgaatg
      961 ttattgtgga aggctcaatg ttgaaatctt tccaaatgga atggatgcta gatgaaattg
     1021 cttcttctga gttaatttat gaagtgaaaa atagccaaga taaagaaaaa actcagcaga
     1081 atatcactgc agaaatggtg caaaccgcat tgcaaaatca ttattttagc accttatatg
     1141 atagtcaaaa tcagccgata aaatttacga caaagcccac agcgactcgc tttgaaatca
     1201 agaataacag agtgattttt tatattactt ttcatttagc taaacctcac gaattaaagc
     1261 agagtaaagt gagattctat acttatgaac cgagttatta tattgcgatg gaatataatc
     1321 gccctgctga tgtcaatacc agtaatgcaa gttgtaaagc aacattggtg caacctcaag
     1381 tagatagtaa gttacgctta tatgcttccg gtttggataa aaaccaatca ctggatatgc
     1441 ctgaaaatgg agattactct cttggtgccc aatttgctca aaaagtggag attatatgcg
     1501 attaaaagca acatattggg gaatgatagt gataggattt ttgagcctat ttgctcttta
     1561 tcagctttat ccaatgatat tgtttaaagt gatggaatgg caaaaaaact ttaatttaca
     1621 gctttccgct tcgctcaatg aattagctga aaatcaaagt aaagccggta ttaccttagt
     1681 atttattagt tttttatatg gagtatttca cgcagttggg cctggtcacg gaaaatttat
     1741 tctgagcagt tatctttctt ttgaaaaaac aaaattaaat caggcgatga aaatcacttt
     1801 tgcctcagca ttggtgcagg gcttagtggc aatttcattg gtgacggtga ttgtggtgat
     1861 ttttactctc tctcgccaat attttaattt aaccctaaaa tgggttgagc gggggagctt
     1921 tggtttgatg atcttgttcg gcatttactg gtgttaccag gtgtatcgaa cggtgaacag
     1981 agcgcgaaag cctatcatta aatccattcg tatttcgcaa aaaaaagctg gaaaattacc
     2041 gcttgtcacg cagaaagggc atattcatac cgaaaattgt agctgtaggc ataaacattt
     2101 accgacttca aatgaaatgc aacaagtgag agattggaaa gcacagctga tgttggtatt
     2161 aagtattggc tcaagacctt gctctggcgc tattcttgtt ttatttcttt cttatacgct
     2221 caatctctat gcttgggggg ttatttcagc attagctatg gcagtaggaa cagggctgac
     2281 tttaagctta tttgcttatc ttgttatcat tgcgagaaat aaggcagtta aggttagcca
     2341 atggtatttt tcagctcaaa ttaatcgaaa tatcgtttgg atattaaaat ttacacttgg
     2401 actcgcatta attctatttg gaatggtatt atttcacagt agtttcattg atgctggtac
     2461 aggcgttttt
//
LOCUS       DANLUQ010000215         2403 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.217, whole genome shotgun sequence.
ACCESSION   DANLUQ010000215 DANLUQ010000000
VERSION     DANLUQ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.217"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            122..1717
                     /locus_tag="PXB27_002454"
     CDS             122..1717
                     /locus_tag="PXB27_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HDL5347191.1"
                     /translation="MQRHEQLAQVQQTQKWDFIIIGGGASGLGIAVDAASRGYKTLLL
                     ESHDFAKGTSSRSTKLVHGGVRYLANGDVALVREALRERGRLAKNAAHLFKNQSFIIP
                     NYKFKDCVMYRVGLGLYDMLSGNLSLGKTENISKTEAKRRLPTLNDTGLRTGTVYQDG
                     QFDDSRLAVNMAQTVVEHGGTVLNHAKVTSLHKTPQGKIDGVTFYDELNQQEHTVFGT
                     AVINATGVFMNDILSMDHGSTKKFVVPSQGIHLVLDKSFLPSNDALMIPKTSDGRVLF
                     AVPWHEVLVVGTTDTLIENPSYEPIPLEQEVNFILETAAQYLTKKPTRDDVKAVFAGQ
                     RPLAAPEKEGQSTKEVSRSHKVLTDATSGLITITGGKWTTYRQMAEDTVEEALKVHSE
                     LGNKPCVTVNLPIHGNIPAAQVNLQDHLYVYGSDIPQLRALMTETPEYAQKIHPRLDY
                     TVAEVIWAVRHEMAQTVEDVLARRVRLLFTDARAAIDSAAKVAAIMAKELGKDEMWQQ
                     QQVIQFLDVAKHYLLVDYQPQPI"
     gene            complement(1804..2103)
                     /locus_tag="PXB27_002455"
     CDS             complement(1804..2103)
                     /locus_tag="PXB27_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347192.1"
                     /translation="MENIKMEELKLTEKELLVLKGNALRMRFKAETNKTKNEIMQPFK
                     QVKQTGLALVNSPAVKTIALDFLVKRLLSMKGISFSVLGLTAFFLLQQKHKRDDS"
     gene            complement(2090..>2403)
                     /locus_tag="PXB27_002456"
     CDS             complement(2090..>2403)
                     /locus_tag="PXB27_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDL5347193.1"
                     /translation="IEINEQKNQLISVLIFILLAFVFLLVSFVSLLFGLDNYLLPEQK
                     VKVFFSIAIIMFIAVTICGMMILSALKKQRHFMQSTLTELKLDIAAFKSALTLKQNGE
                     H"
BASE COUNT          797 a          440 c          463 g          703 t
ORIGIN      
        1 tatagaaata ttttgtgaca tagatctaat ttttaaacaa atgtctttga agtcatttta
       61 ttttcgttta tgctaattaa gtaatgatta aatgttttaa ttcgagcaaa aggagcaaaa
      121 aatgcaaaga catgaacagc tagctcaagt acaacaaacg caaaaatggg attttattat
      181 tatcggtggt ggcgcaagtg gtttggggat tgcggtagat gcagcttccc gtggctataa
      241 aaccttatta ttagaaagcc atgactttgc gaaaggtaca tcgagccgaa gcacaaaatt
      301 agtccacggt ggcgttcgct atcttgcaaa tggcgatgtt gcactcgtga gagaagcatt
      361 gcgtgaacga ggtcgtttag ctaaaaatgc agctcattta tttaaaaacc aaagctttat
      421 tattccaaac tacaaattca aagactgtgt aatgtatcgt gttggtttag gcttatacga
      481 tatgctttca ggcaacctga gtttaggaaa gactgaaaac atcagtaaaa ctgaagcgaa
      541 aagacgttta ccaaccttaa atgatactgg tttacgtaca ggcactgttt atcaagatgg
      601 gcaatttgat gactcacgtc ttgcagtgaa tatggcacaa acggtagtag agcacggcgg
      661 tacagtacta aatcacgcca aagtgacttc actgcacaaa acgccacaag gcaaaattga
      721 tggggtgact ttctacgatg aactcaatca acaagaacat actgttttcg gcactgcggt
      781 gattaacgca accggcgtat ttatgaatga tattttaagt atggatcacg gtagcacgaa
      841 aaaatttgtc gtaccaagcc aagggattca cttagtatta gataaatcat tcttaccaag
      901 taatgatgcg ttaatgattc caaaaacctc tgacggacga gtgttatttg ctgtaccttg
      961 gcacgaagtg ttagtcgtag ggacaacaga tactctgatt gaaaatcctt cttatgagcc
     1021 aattccattg gaacaagagg tgaactttat tttagagaca gccgcacaat atctgacaaa
     1081 aaaaccaact cgtgatgatg taaaagcagt ctttgccggt caacgtccgc ttgcggcacc
     1141 ggaaaaagaa ggtcaaagca ctaaagaagt atctcgtagt cacaaagttc tcactgatgc
     1201 aaccagcggt ttgattacga ttacaggtgg taaatggaca acttatcgtc aaatggcaga
     1261 agataccgta gaagaagcgt taaaagtcca ctctgaacta ggcaataaac cttgtgtgac
     1321 cgtcaactta ccgattcacg gtaatattcc ggcagcacaa gtgaatctac aagatcatct
     1381 atatgtatat ggctcagata ttccacagtt acgcgcctta atgactgaga ccccggaata
     1441 tgcacaaaaa atccatccga gactagatta taccgttgca gaagtgatct gggcagttcg
     1501 ccacgaaatg gcacaaacgg ttgaggatgt attagctcgc cgtgttcgtt tgctcttcac
     1561 tgatgcgaga gcggcgattg atagtgcagc aaaagtggct gctattatgg cgaaagaatt
     1621 aggtaaagat gaaatgtggc aacaacagca agttatccag ttccttgatg ttgcgaagca
     1681 ctatttattg gtagattatc agccacagcc aatctaatac gctaaaagca agcggttaaa
     1741 atcgataaaa atattgcaat tacgttattt gcaacttttt aaaccatttt aaccgcttgt
     1801 atattagcta tcatctcttt tatgtttctg ttgtaataaa aagaatgctg ttaaccccaa
     1861 tacagaaaag ctaattcctt tcatactcaa taatcttttt accaaaaaat ctaatgcaat
     1921 ggttttaact gccggtgaat tgaccagtgc aagccctgtt tgtttcactt gtttaaaagg
     1981 ctgcataatc tcatttttgg ttttattggt ttccgcttta aatcgcattc taagtgcatt
     2041 accttttaga actagaagtt ccttttcggt taatttaagc tcttccattt taatgttctc
     2101 cattctgttt caaagttaaa gcactcttaa atgcggcaat atctaatttt agctcggtta
     2161 atgtactttg cataaaatgg cgttgttttt tcaaggcaga aagtatcatc attccgcaaa
     2221 tagtcacagc aataaacata ataatggcaa tagaaaagaa tactttaact ttctgttcgg
     2281 gtaacaaata attatctaaa ccaaataata aactcacaaa agaaactaat aaaaatacaa
     2341 aagccaataa gataaatata agtactgaaa ttaattgatt tttttgctca ttaatttcaa
     2401 ttc
//
LOCUS       DANLUQ010000216         2369 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.218, whole genome shotgun sequence.
ACCESSION   DANLUQ010000216 DANLUQ010000000
VERSION     DANLUQ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.218"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            17..265
                     /locus_tag="PXB27_002457"
     CDS             17..265
                     /locus_tag="PXB27_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5347194.1"
                     /translation="MKNAINGNFGDHKSLAGTGGLYEMRIATGAGYRIYYAQQGNVIY
                     ILLHGGDKSTQQADIEKAKALWAELKTTGESNGNNTNQ"
     gene            243..566
                     /locus_tag="PXB27_002458"
     CDS             243..566
                     /locus_tag="PXB27_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative addiction module antidote protein"
                     /protein_id="HDL5347195.1"
                     /translation="METIQINEQAAEKHGLRPFDVAEFLTDEATIQAYLAEVLKEGDQ
                     DEFLEALNNVARARGMAELAKQTGLGRESLYKTLAKGSKPRFDTIQKILGAFNLVLIP
                     QVKNG"
     gene            1134..1382
                     /locus_tag="PXB27_002459"
     CDS             1134..1382
                     /locus_tag="PXB27_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HDL5347196.1"
                     /translation="MTATKKTLSDTLLKNLKYSPNAKPIADSNGLFILAQKKAKVWIY
                     SYISPKTGKRSTKNRIGSYPQMSLVEQSPQVFEQIARL"
     gene            1434..2021
                     /locus_tag="PXB27_002460"
     CDS             1434..2021
                     /locus_tag="PXB27_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SocA family protein"
                     /protein_id="HDL5347197.1"
                     /translation="MFDELKIAQATAYLLHKAGGTMEHIKVMKLLYLADRLSWQRYGY
                     SISNDEYYSLPYGPVLSNTLNLMRGEEASLYQKTSIWGEWIADKEDHKISLKKSVNTE
                     DEYFFDCLSESDTEILEEVFATFGNYKPFALVEYTHNPKYIPEWEDPQGRSKPISLST
                     LLTKLGKTPSEIESILEQQAEKGTMDNLFKGLGNA"
     gene            2014..2369
                     /locus_tag="PXB27_002461"
                     /pseudo
     CDS             2014..2369
                     /locus_tag="PXB27_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252950.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
BASE COUNT          851 a          446 c          468 g          604 t
ORIGIN      
        1 aacggtagca aggcggataa aaaatgcgat taatggcaac tttggcgatc ataaatcttt
       61 agccggtaca ggcggacttt atgaaatgcg gatagcaaca ggagccggat atagaattta
      121 ttacgcacaa caaggaaatg taatttatat cttgttacac ggcggcgata aatcaacaca
      181 gcaagccgat atagagaaag cgaaagcatt atgggctgaa ttaaaaacta caggggaaag
      241 caatggaaac aatacaaatc aatgaacagg cagcagaaaa gcacggttta cgcccttttg
      301 acgtggcgga gtttttaacc gatgaggcaa ctattcaagc ctatttagcg gaagtgttaa
      361 aagagggcga ccaagacgaa tttttagaag ccttgaacaa tgtggcaagg gcaaggggaa
      421 tggcggagct tgccaagcaa acagggctag ggagagagag cctatataaa accctagcca
      481 aaggcagcaa accacgcttt gacactattc aaaaaattct aggggcattc aatttagtgc
      541 ttattccaca agtcaaaaat ggctaaaact tatcacttgc tacctgccaa aacctcacat
      601 tcaaatagcc gttgattgat attaacggct tttttattgc ttaattctgt accaattacg
      661 caccgattgc ggatcaatac ggggcaagtt ttcgcaaaac cttaatgaaa tatgaccgct
      721 tgtgattttg tacaaaacga cacaaaccaa tgtataaaat caaaacttgc cacaaatggc
      781 ggaatgatat taaatgcccc acaaactagc agtatttctt aatgactttt cattttaagg
      841 gtacatttaa gggtatgttt aacaagtctt aaatgcactt ctcaataata acaagcctca
      901 tagcatacaa ttcaaactcg gctggggacc atttaagcgt tgcatattgt ctttcactgt
      961 ctcaaattta ctataaaatc aagaacttag attattttat agtctcttat tgtcttcttt
     1021 agttgcattg tatcttagcg tttatgatcc ctaaaatgat ccctattttg aaaacgggaa
     1081 aatttaggga tcataaactt cgataaaccc ttatatatca aggattagaa gaaatgactg
     1141 caactaaaaa aacactttca gacacattac taaaaaatct taaatattcc ccaaatgcga
     1201 agcctatcgc agacagtaac gggctattta ttcttgcaca gaaaaaagcg aaagtatgga
     1261 tttatagcta tatcagcccc aaaacaggca agcgaagcac aaaaaataga atcggtagct
     1321 atccgcaaat gagcttagtg gaacaatccc cacaggtatt tgaacaaatc gcacgtcttt
     1381 aaaatattaa attaagagaa agcactattt gcgatgtagc aggagataaa acaatgtttg
     1441 atgaattaaa aatagcccaa gcgaccgcct atttgctaca taaggcaggc ggaacaatgg
     1501 agcatattaa ggtaatgaaa ttgctttatc ttgcggatcg cttatcgtgg caaagatacg
     1561 gctattctat ttctaatgat gaatattatt cattgcctta tggaccggta ttaagcaaca
     1621 cactcaacct aatgcgagga gaagaagcga gcttatacca aaaaaccagt atttggggcg
     1681 agtggatagc agataaggaa gatcacaaaa tcagccttaa aaaatccgta aataccgaag
     1741 atgaatactt ctttgattgc ctttcagaga gcgacaccga aattttagaa gaggtatttg
     1801 cgacctttgg aaattataag ccattcgccc tagtggaata tacccataac cctaaataca
     1861 ttcccgaatg ggaagaccca caaggcagaa gcaagccgat ttctttaagc acgcttctta
     1921 ctaagctagg gaaaacgcca agcgagatcg aaagcatttt agagcaacag gcggaaaaag
     1981 ggacaatgga taaccttttc aagggcttag gcaatgcttg aaatcaaaag acaagtaacg
     2041 attcttagac cgaccaacta cggcaaggaa gagaaccact acaagcattt atatattctg
     2101 cttaccgatc cgtgctttga tggtgaaacc ggtaaaggag atatgatttt aagtgtaaac
     2161 tgctcaagca ttaaagacgg gaaagcactt gatgaaactt gtattttaaa ggcaggaaca
     2221 cacgaattta tcacacggga cagcttcatt ttctaccgcc atataaaaat tgattcgtta
     2281 gatgagatta aggcaaatat agaagccggt tattttattc aaaagcccta attaatgatg
     2341 aagcctatca acaaatacta aaaggcgta
//
LOCUS       DANLUQ010000217         2233 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.219, whole genome shotgun sequence.
ACCESSION   DANLUQ010000217 DANLUQ010000000
VERSION     DANLUQ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.219"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            39..467
                     /locus_tag="PXB27_002462"
     CDS             39..467
                     /locus_tag="PXB27_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014992182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDL5347198.1"
                     /translation="MTHDWLYWALASALFAALTAIFAKVGLQGVDSDFATFIRTVVII
                     IALIAFLTYSGKWQPLDSLSSKNWLFLILSGLATGLSWSAYFKALQMGNASQVAPIDK
                     FSLVLVTLFAVIFLGERPSGQDWLGIGLVAGGVLLLAFKR"
     gene            473..1195
                     /locus_tag="PXB27_002463"
     CDS             473..1195
                     /locus_tag="PXB27_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HDL5347199.1"
                     /translation="MQNFANIEIAFIHRKAPLPSDFSYPEMPGGLTEKQVQKWQSRRV
                     AFFLLTELFKKHHLDLDLLADMQRSPSGRTFVDSEQIDFNISHSGEWIAVIFSYSFAK
                     LAVGIDIEHPQKIRRYHDLICHYGNAEEQNVLLANHSPILSNLAERFYLSWCLREAIL
                     KSQGVGIVKLSEVKHLPLEKQIFSAHCPKGKLHFVSELPFYLSYFYQQPQNMLLSEPL
                     LYHWQNGQFQPIKCQSLVYDVN"
     gene            1205..1624
                     /gene="psiE"
                     /locus_tag="PXB27_002464"
     CDS             1205..1624
                     /gene="psiE"
                     /locus_tag="PXB27_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HDL5347200.1"
                     /translation="MPKKSLEEHHSSFGKIASETFQWIMNIALLVTGALLSYSLFAEA
                     YSLYELSFSDSEKFQIVEKIVIFFLYFEFLALIVKYFKSNYHFPLRYFLYIGITAMVR
                     LIIVDHSNALHTLLFALSILVMIIALYIVHTDRLHKS"
     gene            1633..>2233
                     /locus_tag="PXB27_002465"
     CDS             1633..>2233
                     /locus_tag="PXB27_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDL5347201.1"
                     /translation="MKLTQYQHNDLQILNRETVYKGHFELQKITFRHKLFSGEMSGEV
                     VRELLIKGEAAALIAYDPTLDKVVLIEQVRIGAYDAGQKKSPWLLELVAGMVDKGNEE
                     PAEVAMREAKEEAGLEVTTVQHALSVWDSPGGQLERLHLFLGLIDSTQVKSGEVHGLL
                     EEENEDILVHVVSREQAYQWVEDGTIDNVIAVIGLQWLQL"
BASE COUNT          637 a          450 c          475 g          671 t
ORIGIN      
        1 tatgattaga aattaaccta aaatattaaa attttgttat gacacacgat tggctctatt
       61 gggcattagc ctctgcactt tttgcagcat taaccgccat ttttgccaaa gttggcttac
      121 aaggcgtaga ttctgatttc gccactttta ttcgcacggt agttattatt attgcactta
      181 ttgcctttct cacttacagc ggaaaatggc agccgttaga tagcttgagc agcaaaaact
      241 ggctgttttt aatcttatcc ggcttggcaa caggattgtc ttggtcggcg tatttcaaag
      301 cattgcaaat gggtaatgct tcccaagtcg ctccaatcga taaattcagc ttggtgttag
      361 ttaccctttt tgcagtaatt ttcttaggcg aacgcccaag cggacaagat tggcttggca
      421 ttggcttagt tgctggtggc gtactgttac ttgccttcaa gcggtgattt ttatgcaaaa
      481 ttttgcaaat attgaaattg cgtttattca tcggaaagca cctctgccga gtgatttctc
      541 ctaccctgaa atgcccggcg gtttaactga aaaacaggtg caaaaatggc aaagccgccg
      601 agtcgccttt tttctgctga cggaattgtt taaaaaacac catctggatt tagatttatt
      661 agctgatatg caacgctccc caagcggtcg cacttttgtc gattctgagc aaattgactt
      721 taacatcagc cattcagggg aatggatagc ggtcattttt tcgtattctt ttgcaaaatt
      781 agcggtcggc attgatattg aacacccaca aaaaatcaga cgttaccacg atttaatctg
      841 ccattacgga aacgctgaag aacaaaatgt tttattagcc aaccactccc ccattctaag
      901 caacctcgcc gaacgctttt atttgagctg gtgcttgcga gaggcgatat tgaaatcgca
      961 aggggtgggg attgtgaaac tgtccgaagt aaaacattta ccgttagaaa aacagatctt
     1021 ttccgcccat tgtccgaaag gcaagctgca ttttgtgagc gaactgccgt tctatttaag
     1081 ctacttctat caacaacctc aaaatatgct attatctgaa cctttgctat accattggca
     1141 aaacgggcag tttcagccta taaagtgcca atcacttgtt tatgatgtta attaaggata
     1201 acgaatgcct aaaaaatctt tagaagaaca ccattccagt tttggcaaaa ttgcttccga
     1261 aacctttcag tggattatga atattgcctt gctagtaaca ggagcattgc tttcttactc
     1321 gctgtttgct gaagcctatt cactctatga actttccttt agcgacagtg agaaattcca
     1381 aattgtagaa aaaatagtaa ttttctttct ctattttgag tttcttgcgt tgattgtgaa
     1441 atatttcaaa tcgaattatc actttccgtt acgctatttc ctctatatcg ggattactgc
     1501 catggtgcgt ttgattattg tcgatcattc taatgcgttg catacactat tattcgcact
     1561 ctctattttg gtgatgatta tagcattata tattgtacac accgatcgtt tacataaaag
     1621 ctaggaacta atatgaagct aacccaatat cagcacaatg atttacagat tctaaaccga
     1681 gaaaccgtct ataaagggca ttttgagcta caaaaaatca ctttccgcca taagctgttt
     1741 tccggtgaaa tgagcggtga agtggtgcgt gaactcttaa ttaaaggcga agcggcggct
     1801 ttaattgcct atgatccaac cttagataag gtagtattaa ttgagcaagt gcggattgga
     1861 gcttacgatg caggacagaa aaaatcccct tggttgctgg aattggttgc cggtatggta
     1921 gataaaggca acgaagagcc ggcagaagtg gcaatgcgtg aagccaaaga agaagccggc
     1981 ttggaagtca caacagtgca acacgcctta agcgtatggg acagtcccgg tggacaactg
     2041 gaacgcttgc acctgtttct cggcttgatt gacagcaccc aagtgaaatc cggcgaggta
     2101 cacggcttac tggaagaaga aaatgaagac attttggttc acgttgtaag ccgtgaacag
     2161 gcatatcaat gggtagaaga cggcacaatt gacaatgtga ttgcggtaat tgggctacaa
     2221 tggttgcaat taa
//
LOCUS       DANLUQ010000218         2211 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.220, whole genome shotgun sequence.
ACCESSION   DANLUQ010000218 DANLUQ010000000
VERSION     DANLUQ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.220"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..1190
                     /locus_tag="PXB27_002466"
     CDS             <1..1190
                     /locus_tag="PXB27_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL5347202.1"
                     /translation="DTLLKNLKYSPNAKPIADSNGLFILAQKKAKVWIYSYISPKTGK
                     RSTKNRIGSYPQMSLAEARLKRDELNTLVLQGLDPFEHLAKMQREAKEKQESIQSFAY
                     KWADWKLSKGKCKPKTMEKCLQRLENHLFPRFEGYTLEDFTLTDTINRLTDLDRIMPD
                     TLHRIAGNLIEILDYAVLCGRINFNPISVIKKAFSSVSTTHQPAIMPEELAEFMQDLQ
                     KSNRTPQIKLLVEWQLLNILRPFEAVAVQWSDIDWETKTLNIPADRMKGGKNPHSVPL
                     TKQAIAILEEMKKYNGHHKHIFTHRTDRNKPANSQTVNNAIKRLSGGKYKGLLVAHGL
                     RSIASTYLHERFTTETLVIEACLSHTNKDTVMSAYHRKNWLDRRRGIMQEWADFVEQC
                     KSI"
     gene            1660..1866
                     /locus_tag="PXB27_002467"
     CDS             1660..1866
                     /locus_tag="PXB27_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDL5347203.1"
                     /translation="MENTPAKQRFMKIDEVSAVTTLSETQIYRKMKAGDFPQSISIGA
                     NSKVWLESEIQQWIQRKIEQHRNQ"
     gene            1876..>2211
                     /locus_tag="PXB27_002468"
     CDS             1876..>2211
                     /locus_tag="PXB27_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347204.1"
                     /translation="MTLETFNQQKAKFHEQEKAVLAIQTELDKAKNILEALQNEKAEF
                     VARQKTNLANIGTLSADEYVEIKNKNSGLQARIEYYQALIVDLENKCYAEQEILFQQQ
                     NELKQIRRII"
BASE COUNT          834 a          456 c          410 g          511 t
ORIGIN      
        1 cagacacatt actaaaaaat ctcaaatatt ccccaaatgc gaagcctatc gcagacagca
       61 acgggctatt tattcttgca cagaaaaaag cgaaagtatg gatttatagc tatatcagcc
      121 ccaaaacagg caagcgaagc acaaaaaata gaatcggtag ctatccgcaa atgagcttag
      181 ccgaagcacg cttaaagaga gacgaactaa atacccttgt tttacaaggc ttagaccctt
      241 tcgagcattt agccaaaatg caaagagaag ctaaggaaaa gcaagaatct attcaatctt
      301 tcgcctataa atgggctgat tggaagctat caaagggaaa atgcaagcct aaaacaatgg
      361 aaaaatgctt acagcggtta gaaaatcatt tattcccacg ttttgaaggc tatacgctgg
      421 aagattttac cttaaccgat acaatcaacc gcttaacgga cttagatcgg ataatgcccg
      481 acactttgca cagaatagca gggaatttaa tagaaattct tgattatgcg gtattgtgtg
      541 ggagaattaa ttttaaccca atcagcgtca ttaaaaaggc gttttccagc gtaagcacga
      601 cacaccagcc agcaataatg cccgaagagt tagccgaatt tatgcaagac ttgcagaaat
      661 caaatagaac gcctcaaatt aaattattag ttgaatggca actcttgaat attcttagac
      721 cgtttgaagc cgtagcggtg caatggagcg atatagattg ggaaactaag acgctcaaca
      781 ttccggcaga tagaatgaaa ggcggaaaaa atccgcattc tgtaccgcta acaaaacaag
      841 ctatcgcaat cctagaggaa atgaagaaat ataacggaca ccataaacat atttttacac
      901 accgaacaga ccgaaacaaa ccggctaata gccaaaccgt gaataatgca ataaaacgct
      961 taagcggtgg aaaatataaa gggcttttag tggctcacgg tttaagaagc attgccagca
     1021 cctatttaca cgaacgtttt actactgaaa cgctagtaat tgaggcttgt ttatcccaca
     1081 cgaacaaaga caccgtaatg agtgcatacc accgtaaaaa ctggctagac cgcagacgtg
     1141 gaataatgca ggaatgggcg gattttgtag aacaatgcaa gagcatttaa atgacaaagg
     1201 cggagcaatc cgcctttctt tttgcttgat gaaaaatttt cattttttcc tatcagctcc
     1261 cagcccgtgc cgtctatccc acgcctcgcc cgcacactta aagtctcgat ttctacgcaa
     1321 atgcaaaatt aagcccaaac attgccccgt atagcgtgaa aaactttcat atagccgttt
     1381 tttattacgc aattttacgc aagaaaacgc aaatttacgc aggcagaaac accactttta
     1441 aaaactcgat gacagaacga tgaaagaacc gctgaaaact tgcaaaaaat cagagaaaaa
     1501 taaccgcttg caagttttcc aaatcagaat taagcccctc taatctgtaa aatctccttt
     1561 cacagcccta acaaatagca aaaaatccaa gatgatttat tctttcacac gttagcaggc
     1621 aatagcacgc caaagcaaat ttttacggag taaaacaaaa tggagaacac accagcaaaa
     1681 cagcgattta tgaaaataga tgaagtttca gccgtaacta cgctttcaga aacgcagatt
     1741 tatcgcaaaa tgaaagccgg cgattttccc caaagtatca gtattggggc aaatagcaaa
     1801 gtatggctag aaagtgaaat ccagcaatgg attcaacgca aaattgaaca acaccgcaac
     1861 caataagggc aaattatgac acttgaaaca tttaaccaac aaaaagctaa attccacgaa
     1921 caagaaaaag ccgttttagc tattcagacc gaattagaca aagcgaaaaa tattcttgag
     1981 gcattgcaaa acgaaaaagc ggaatttgtc gcacggcaaa aaacgaattt agccaatatc
     2041 ggcacgcttt cagcagatga atatgttgaa atcaaaaata aaaatagtgg tttacaagca
     2101 cgcatagaat attatcaagc gttgattgtt gatttagaaa ataaatgcta cgcagagcaa
     2161 gaaatattat tccaacagca gaatgaacta aaacaaatca gacgtataat t
//
LOCUS       DANLUQ010000219         2168 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.221, whole genome shotgun sequence.
ACCESSION   DANLUQ010000219 DANLUQ010000000
VERSION     DANLUQ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.221"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            82..378
                     /locus_tag="PXB27_002469"
     CDS             82..378
                     /locus_tag="PXB27_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347205.1"
                     /translation="MPFDCQGAFWLGYYQYCKLSNDVKNYTSKELSIIGESLYGTQWQ
                     SNLARDLRLSDARRVREWVAGERKIPFGVWADLTELVKAKKANLSSILKKLTID"
     gene            complement(525..836)
                     /locus_tag="PXB27_002470"
     CDS             complement(525..836)
                     /locus_tag="PXB27_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347206.1"
                     /translation="MTKTKHKHPLAEAFYQLGKDVGEINRRLVVVDEQSLKLQELKQT
                     INEQTEQIKTLGTVVNKLIDALQQSQSLNVRSESAVAATENQDHLNDGVQYQGPGRAR
                     A"
     gene            complement(829..978)
                     /locus_tag="PXB27_002471"
     CDS             complement(829..978)
                     /locus_tag="PXB27_002471"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347207.1"
                     /translation="MDNILITIHPTETIDITIDKLVVIHIGEEYGDTPDFLTIYQQAK
                     ELNDD"
     gene            complement(1212..1505)
                     /locus_tag="PXB27_002472"
     CDS             complement(1212..1505)
                     /locus_tag="PXB27_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347208.1"
                     /translation="MTTFNKILNPMYSTIASYSTQDDGSLNAKYVVGTGDDTDGEVTN
                     FVIITSEYKYIDAQSAKAITDAPLTKEDIGKTPTQIMLGRIYNHLKETGQIVV"
     gene            complement(1628..>2168)
                     /locus_tag="PXB27_002473"
     CDS             complement(1628..>2168)
                     /locus_tag="PXB27_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5347209.1"
                     /translation="NVNNLAAISANLGKVTAGTITGTTITGNTISGGSINGTTISGTT
                     ISGGTIKGARLEGATGKFTGELEVSQLIGGGVIEQVTGKLKYSHRNRIYEGQEHGENS
                     SSPIYRHEYIYKATVTINAAPTRRYIEIIGSESFILEANQSITKTIEKSLNRNASGSS
                     LDFIVLAYSISTAKYISIS"
BASE COUNT          591 a          422 c          426 g          729 t
ORIGIN      
        1 agagagctat ctattaacaa tggagagata gcaagattaa tggataaaat accaggcgac
       61 ctcgaggacc ctaaagacgg gatgcctttt gattgtcagg gggcgttttg gcttggatac
      121 tatcaatact gcaaattaag taacgatgta aaaaattaca catcaaaaga gctatccatc
      181 attggcgagt cattgtatgg cactcaatgg cagtctaatc tagcgagaga tttgagatta
      241 tctgatgctc gccgtgtccg cgaatgggtg gctggtgaac gtaaaatccc tttcggggtt
      301 tgggctgatt tgaccgaatt ggttaaagcc aaaaaagcta acttatcatc aattcttaaa
      361 aaattaacaa tagattagca ctgattttaa agcccctctc ggggcttaat tatagaaaat
      421 aaaatcacaa tggctaattt tgccaaattt gcctgttaaa acgtgcgaaa aattacaaaa
      481 aacggctaga gctatagccg ttttgagtgc agatgttaac agagttaagc tctagcacgt
      541 cctgggcctt gatactgcac accatcattt aaatgatctt gattttcggt tgctgctaca
      601 gcagattctg atcttacatt taaactttgt gactgttgta atgcatcaat aagtttattt
      661 accactgttc cgagtgtttt aatttgctcg gtttgctcat taatagtctg ctttaactct
      721 tgcagtttta atgattgctc atccacaacc actaatcgac ggttaatctc tcctacgtct
      781 ttacctaatt gataaaatgc ttctgcaagt gggtgtttgt gtttagtttt agtcatcgtt
      841 aagctccttt gcttgttggt atatagttaa aaagtcaggt gtgtcaccat actcctcacc
      901 tatatggata actaccagtt tgtctatggt gatatcaata gtttcggttg gatgtatagt
      961 gattaagata ttgtccatat tcccgcctta aatttgggtt tggtcaggga taagttggat
     1021 cgtgccgtag tactgccatt ttgggtggat agctcaattt gaggctctgt gctgccctct
     1081 actatggcgt gtaaatccag cctaataccc tctaaattta caggctcgcc atttgtttgc
     1141 ctaaaaaaaa aggcgataac ttacgtcatc gcctctgaaa aacttaaatt tatgcttttg
     1201 catttagacc cctacactac aatctgaccg gtttctttga ggtggttgta gatacgtcct
     1261 aacataatct gcgttggcgt tttgccaata tcctcttttg ttagcggtgc atcagtaatc
     1321 gccttggcgg actgtgcatc aatgtatttg tactcacttg tgatgattac aaagtttgtt
     1381 acttctccat cggtatcatc gcctgtaccg accacatatt tagcattgag cgagccgtca
     1441 tcttgcgttg agtagcttgc gatggtggaa tacattgggt ttaagatttt gttaaaagtt
     1501 gtcataattt ttccttttat cgatagttga aataaaaaca atcgcctgaa ttgacctgca
     1561 agcggttggt tttggatatt taaaaaagct gtggctatcc gcactcagta ctacattgag
     1621 gcgataacta actgatagag atatatttag ccgtcgaaat ggaataggct aaaacaataa
     1681 aatctaaaga gctaccactt gcatttctat ttaatgattt ctctattgtt tttgtgattg
     1741 attgatttgc ttctaaaatg aatgactcac ttccgatgat ttcaatatac ctccttgtcg
     1801 gcgctgcatt tatggtgaca gttgctttat agatatattc gtgtctataa attggagagg
     1861 aggagttttc tccatgttct tgcccctcat aaatgcgatt acgatgtgaa tattttaatt
     1921 tgcctgtaac ctgctctata acacctcccc ctattaattg agatacttca agttcgccgg
     1981 taaatttgcc agtagccccc tccaatcttg caccctttat tgtgccgcca gagatagttg
     2041 taccgctaat tgttgtacca ttaatacttc cgccactaat tgtattacca gtaattgtcg
     2101 tacctgtaat tgtaccagca gttaccttac caagatttgc cgatattgcc gctaaattgt
     2161 ttacattt
//
LOCUS       DANLUQ010000220         2155 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.222, whole genome shotgun sequence.
ACCESSION   DANLUQ010000220 DANLUQ010000000
VERSION     DANLUQ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.222"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            10..357
                     /locus_tag="PXB27_002474"
     CDS             10..357
                     /locus_tag="PXB27_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347210.1"
                     /translation="MFREEAIEYQSKKWKSTAVLFSRVPALVVFSISFFIFTAFILFI
                     VFGNYTRRETVVGELVMQSHPILLSAPKSGYISENYIQPHQQVKKRAIRWLKLRWIEL
                     RIAKILMLILFFR"
     gene            354..1271
                     /locus_tag="PXB27_002475"
     CDS             354..1271
                     /locus_tag="PXB27_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HDL5347211.1"
                     /translation="MKSQIQATEQAITALYKNKSETINSLEKQIKNSQKIYQDKQSYL
                     VEIEKSMNDYAELVKRYEKLLKVGHSSHDEVNIQKSRYFQQKSLFNELKQELIQLKST
                     QLSLENEIETRKTEFNNQIIRYEMQKSDLNIRLMEFESVSDIIVNASLDGKIESTSVT
                     VGQIIKENDPLAQILPQNKGNYQLVMWVPNSAISFIKQGDEVNIRYEAFPFEKFGQFK
                     GKITSISTLPASLQELSFYKNLPVNLEQGIPLYKILIELADQNVKYNDTSLYFMSGMK
                     AEATLFLEKRKLYEWIFFPMYQLRKNMEQ"
     gene            1271..2155
                     /locus_tag="PXB27_002476"
                     /pseudo
     CDS             1271..2155
                     /locus_tag="PXB27_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253091.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V synthesis protein"
BASE COUNT          721 a          355 c          374 g          705 t
ORIGIN      
        1 tggtattata tgttcagaga agaagctatt gaatatcaat cgaaaaaatg gaaaagcaca
       61 gctgtactat ttagcagagt tccagctttg gttgtctttt ccatttcttt ctttatcttt
      121 actgccttta ttctatttat tgtgtttggt aattataccc gccgtgaaac ggttgtgggt
      181 gaattagtga tgcaatctca tccgatttta ttatctgccc caaaatccgg ctatatatcc
      241 gaaaattata ttcaaccaca tcagcaggta aaaaaaaggg cgatccgctg gttaaaatta
      301 cgctggatag aattacgcat agcgaaaata ttaatgttaa ttctattctt tcgttgaaat
      361 cacaaattca agcaacagaa caagcgataa ctgctttata taagaataaa tcagaaacta
      421 ttaatagcct tgaaaagcag atcaaaaaca gtcaaaaaat ttatcaagat aagcagagtt
      481 atttagtgga aattgaaaaa agtatgaatg actatgcgga gttggttaaa cgctatgaga
      541 aattattaaa agtagggcat tccagccacg atgaagtgaa tattcagaaa agccgttatt
      601 tccaacaaaa atcactgttt aatgagctaa agcaagaatt aattcaatta aaatccaccc
      661 aacttagctt ggaaaatgaa attgaaaccc gtaaaaccga atttaataat cagattattc
      721 gctatgaaat gcaaaaaagc gatttaaaca ttcgtttaat ggaatttgaa tctgtttcag
      781 atattattgt taatgcctct ttagatggaa aaattgaaag taccagcgta acagttgggc
      841 aaattattaa agaaaatgac ccgcttgcac aaattctgcc acagaataaa ggaaattatc
      901 agctagtgat gtgggtaccg aatagtgcca tttcatttat taaacaaggc gatgaggtga
      961 atattcgcta tgaggctttt ccgtttgaaa aattcgggca gtttaagggc aaaattacgt
     1021 caatttcaac cttaccagct tctttgcaag aacttagttt ttataaaaat ctccctgtaa
     1081 atttagagca aggtattccg ctttataaaa ttctgattga gcttgccgat caaaacgtaa
     1141 aatataatga tacctccctt tattttatga gtggaatgaa agccgaagcc acgttatttt
     1201 tagaaaaacg taaactgtat gagtggatat ttttccctat gtatcaatta cgcaaaaata
     1261 tggagcaata atgacaggaa ttaagcagtt aaaatttgat tttcggagaa gaatacctgt
     1321 tattttgcaa actgaaacag ctgaatgtgg cttagcctgt ttagcgatga tgttgggcta
     1381 ttacgataaa aacaccaatt tatttgaatt gcgaagtcaa tatggtactt cctctcgtgg
     1441 cgtaacgctg catactttaa tttctattgc aaatgactgt ggtttaataa ctcgccctct
     1501 ttctctagag ttagatgaag taccacaact tcgtctgcct tgtattcttc attgggattt
     1561 taatcatttt gtagtattaa ctcacgcaag tgaaaaacag tttattatcc acgatcctgc
     1621 tttcggtaaa cgtaaattat cccgtgctga attttccaac cattttaccg gagtcgcttt
     1681 agaagtttgg tctgaagtta aatttgagaa aagcaacaat gaaaatacca ttagtttgta
     1741 tgaaacctta aagcatattt ctggcataaa aggtgcatta gtgaaaattt ttgccctttc
     1801 aatgctcatt gaactcattg ccttattgat gccaattgga acacagttag tgatggatca
     1861 cgttatgcaa gcgaaagatc aatcattact tttaattatc tgtattgggc tatttttatt
     1921 tacccttttt cgcacaatgg tcagtatgtt tagggcttgg gtttccttga aaatgaatta
     1981 tttaattgat tttcaatgga cagccagttt cttttctcat ctcttaaaat taccgcttga
     2041 tttctttgaa aacgccaagt aggtgatatt cattcccgat ttagttcatt aaaaaccatt
     2101 cagaaaactc taacttcaag cattattacc acgattattg atacgattat gataa
//
LOCUS       DANLUQ010000221         2048 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.223, whole genome shotgun sequence.
ACCESSION   DANLUQ010000221 DANLUQ010000000
VERSION     DANLUQ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.223"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            156..1106
                     /gene="coaA"
                     /locus_tag="PXB27_002477"
     CDS             156..1106
                     /gene="coaA"
                     /locus_tag="PXB27_002477"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDL5347212.1"
                     /translation="MSLEKTSKITPFLTFDRQQWADLRKSVPLKLTEQDLKPLLGFNE
                     DLSLEEVRTIYLPLARLIQYYIEENLKRQTVLHRFLGIEPPKVPYIISIAGSVSVGKS
                     TSARILQALLSHYPKERKVDLITTDGFLYPLATLTEKNLLKKKGFPESYDIHSLIRFV
                     SDIKSGKRNVKAPIYSHLTYDIIPNQFNTVDQPDIVILEGLNVLQSGMNYPSSPHNVF
                     VSDFVDFSIYVDAEESLLKKWYINRFLKFRRSAFADPNSYFHHYASLSEQEAIETAST
                     IWEEINGLNLRQNILPSRERANLILIKGQDHAIETVKLRK"
     gene            1259..1798
                     /locus_tag="PXB27_002478"
     CDS             1259..1798
                     /locus_tag="PXB27_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005598787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HDL5347213.1"
                     /translation="MNKKFLLPLILFLALSVAFLVQLARNSQGDDPKKLESALIGKTV
                     PSFNLESVLDEKQTIDQNIVKTGKPRLLNVWATWCPTCYAEHEYLTILAKQGVEIVGI
                     DYKDERPKAIKFLANYGNPYKAIIFDPKGSLGLDLGVYGAPETFIIDGNGVIQYRHAG
                     DVNDQVWNQVLKPIYDGLK"
BASE COUNT          649 a          412 c          347 g          640 t
ORIGIN      
        1 tcgattttta aatatacaaa atgactattt tttgtaaaat acaacggatt ttctctgcat
       61 aactacggca aaataagtgc atatattgta tagttacgcc cttaatttgg tatttaatta
      121 ccttaatatt ccagcaaaaa attgagattt tggctatgag ccttgaaaaa accagcaaaa
      181 ttactccttt tctcactttt gatcgccaac aatgggctga tttacgcaaa tccgttccat
      241 tgaagctgac cgagcaagac ctaaaaccgc ttttaggctt taatgaagat ctttcattag
      301 aagaagtacg cactatttat ttaccgcttg cccgtttaat tcaatactat atagaagaaa
      361 acttaaaacg ccagacagtc ttacatcgct ttctcggcat tgaaccgcca aaagtccctt
      421 acattattag tattgccggc agcgtatcgg ttgggaaaag tacctctgcc cgtattctgc
      481 aagctctgct ctcccattac cctaaagagc gaaaagtgga tttaattacc accgacggtt
      541 ttttatatcc acttgcaacg ctaaccgaaa agaacttact caagaaaaaa ggctttccgg
      601 aatcttatga tattcacagc ctgattcgct ttgtatctga tattaaatcc ggcaaacgca
      661 atgtgaaagc tccgatttat tcacatttga cctacgatat tatcccaaat caatttaata
      721 cggtggatca acccgatatt gtgattttag aagggctaaa cgtgttacaa agtggaatga
      781 actacccgtc cagcccacat aatgtatttg tatcggactt tgtggatttt tctatttatg
      841 tggatgccga agaaagtttg ctgaaaaaat ggtatatcaa ccgtttcttg aaattccgcc
      901 gcagtgcctt tgccgaccca aactcctatt tccaccatta tgcgagtttg tccgagcaag
      961 aagcgattga aaccgcctca acgatttggg aagagatcaa cggcttaaat ttacgccaaa
     1021 atattctccc aagccgagag cgtgccaacc tcattttaat caaagggcaa gaccacgcca
     1081 ttgaaacggt aaaattacgc aaataatatt ttgcaaataa ttgggctttt tcgaccgctt
     1141 gtaatacctt aaaatccgtt gaataccact caacggattt tttatttaaa tcaatgctta
     1201 ttcaactatt ttttggtaag ctaacgactt tgagagtgat tgatattagg catatacaat
     1261 gaacaaaaaa tttctacttc ctttaattct ctttttggcg ttatctgtgg catttttggt
     1321 tcaactcgct cgcaactcgc aaggcgatga tccgaaaaaa ttagaatcag ccttaattgg
     1381 caaaacagtt ccgagcttta atttagaatc ggtattagat gaaaaacaga caatcgacca
     1441 aaatatcgtc aaaaccggca aacctcgcct gcttaacgtg tgggcaactt ggtgtccgac
     1501 ttgttatgcc gaacacgaat acttaaccat tttggcaaaa caaggggtgg aaattgtcgg
     1561 tattgattat aaagacgaac gcccaaaagc gattaaattc ttagccaatt acggcaaccc
     1621 ttataaagcg attatttttg acccgaaagg ctcactcggt ttagatttag gtgtttatgg
     1681 tgcacccgaa acctttatta ttgacggcaa cggcgtgatt caataccgcc acgccggcga
     1741 tgtaaacgac caagtgtgga accaagtgct taaaccgatt tatgatggat taaagtaaat
     1801 gtttgtattt cctgctgaca ttaagaaatc tataaaaagc aattatcaaa gagaatataa
     1861 ctttattttt ataatagttg gtttatcaac tattatagga ataattctaa ctattttatc
     1921 ctcattttta ttgctaaact atttagttag cattgatgca agaaatttat tcattaactc
     1981 attaaacagt aacttctcaa ccatatttat tcctatagca ttatttttat ttttccctat
     2041 aatgctga
//
LOCUS       DANLUQ010000222         2038 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.224, whole genome shotgun sequence.
ACCESSION   DANLUQ010000222 DANLUQ010000000
VERSION     DANLUQ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.224"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..293
                     /locus_tag="PXB27_002479"
     CDS             <1..293
                     /locus_tag="PXB27_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5347214.1"
                     /translation="GTIAARNMAANSINASHIVGSSITADKLNVNNLAAISANLGAIT
                     AGSININNRFKVSNAGVVEMRANSGNVGMVMNNNSIIVYDEQGRVRVKMGKL"
     gene            293..736
                     /locus_tag="PXB27_002480"
     CDS             293..736
                     /locus_tag="PXB27_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005620879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347215.1"
                     /translation="MLIGILCIIALAVLGLLFYFHKKKRKKVNKGVNMAEYGIQTFDT
                     ANVSTFSTNERLFKYIGTKNIKYGKFSFTVTEKYEGQLVFIISMLHSVHRKQATGYKT
                     NIPNLIHLENVSINDKTISGEVVCIFPFSLPADDSDYIQIVYGAY"
     gene            737..1336
                     /locus_tag="PXB27_002481"
     CDS             737..1336
                     /locus_tag="PXB27_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347216.1"
                     /translation="MSYGLSTFNINENFRSLSLQRKGRANFSNKIATIQAASDEIIVI
                     KYSDGQVCLLYRENGIATLATENATFAEYLIFSTNVPKSGGYGIEVYNSAGNVVFSSN
                     HKFLRPIEYVDLNPSKNVFRFTGQANRQYGIILSNFGFDFVINYDNGYSTSRSAKVSG
                     NFIEFGFCKYHYSGLYKTGMSYNSKAMYFNALVVDITGY"
     gene            1451..1744
                     /locus_tag="PXB27_002482"
     CDS             1451..1744
                     /locus_tag="PXB27_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347217.1"
                     /translation="MTTFNKILNPMYSTIASYSTQDDGSLNAKYVVGTGDDTDGEVTN
                     FVIITSEYKYIDAQSAKAITDAPLTKEDIGKTPTQIMLGRIYKHLKETGQIVV"
BASE COUNT          720 a          312 c          390 g          616 t
ORIGIN      
        1 ccggcacgat tgcagctcga aatatggcag caaactcgat taatgcaagt catattgtag
       61 ggagttcgat cactgcggat aagctaaatg taaacaattt agcggcaata tcagcgaatc
      121 tcggagctat tacagctggt tctattaata tcaataatag attcaaggta agtaatgctg
      181 gagtagtcga aatgcgagcc aatagtggaa atgttggtat ggtaatgaat aacaatagta
      241 ttattgttta tgatgaacaa ggtagggtta gagtaaaaat ggggaaatta tgattctaat
      301 aggtatttta tgtattattg cattagcagt cttagggctg cttttttatt tccataaaaa
      361 aaaacgaaaa aaagtaaata aaggggtaaa tatggctgaa tatggtattc aaacttttga
      421 taccgctaat gtctctacat ttagtaccaa tgagcgatta tttaaataca ttggaactaa
      481 aaatattaag tatggtaaat tttcattcac ggtaacagaa aagtatgaag gacaacttgt
      541 atttatcatt agtatgttac attcagttca ccgaaaacaa gcaacaggtt ataaaacgaa
      601 tattcccaat cttattcatt tagaaaatgt ttcgatcaac gataaaacga tttcaggaga
      661 agttgtttgt atttttcctt ttagtctacc ggctgatgat tcagattata ttcagattgt
      721 atatggagca tattaaatga gttacggact atctactttt aacatcaatg aaaatttcag
      781 aagtttatcc ttacaaagaa aaggacgagc taacttttct aataaaattg caactattca
      841 ggcagcgtca gatgaaatta ttgtcattaa atattctgat gggcaagtat gtttgctata
      901 tcgagaaaat ggtattgcca cattagctac agaaaacgca acatttgctg aatatctcat
      961 ttttagtaca aatgtaccga aaagcggagg ttacggcata gaggtttata actcagcagg
     1021 aaatgttgta ttttcatcaa accacaaatt tttaagacct attgaatatg tcgatctaaa
     1081 tccatcaaaa aatgttttta gatttacagg gcaagcaaat aggcaatatg gcattatctt
     1141 atccaatttt ggctttgatt ttgtcattaa ctacgataac ggatattcaa catctagatc
     1201 tgccaaagta agtggtaatt ttattgaatt tgggttctgt aaataccatt attctggttt
     1261 gtataagacg ggaatgagtt ataattcaaa agcaatgtat tttaatgctc tggttgtaga
     1321 tataacaggc tattaaacat taaacgcctt catttaatag aggcgaacta aaattctgat
     1381 tacacattca tcgccgcttg tagcaataca agcggtcttt ttttatcaac aatccacaaa
     1441 aggaaaaatt atgacaactt ttaacaaaat cttaaaccca atgtactcaa ctatcgcaag
     1501 ttactcaact caagatgacg gctcactcaa tgctaagtat gttgttggca caggtgatga
     1561 taccgatggc gaggtaacaa actttgtaat catcacaagt gagtacaaat atattgatgc
     1621 tcaatcagcc aaagcgatta cagatgcacc gctaacaaaa gaggatattg gcaaaacgcc
     1681 aacgcagatt atgttaggtc gtatctataa gcacttaaaa gagacggggc agattgtagt
     1741 gtaggggtct aaatgcaaaa gcataaattt aagtttttca gaggcgatga cgtaagttat
     1801 cgcctttttt ttaggcaaac aaatggcgag cctgtaaatt tagagggtat taggctggat
     1861 ttacacgcca tagtagaggg cagcacagag cctcaaattg agctaaccgt cgattagtgg
     1921 ttgtggatga gcaatcatta aaactgcaag agttaaagca gactattaat gagcaaaccg
     1981 agcaaattaa aacactcgga acagtggtaa ataaacttat tgatgcatta caacagtc
//
LOCUS       DANLUQ010000223         2037 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.225, whole genome shotgun sequence.
ACCESSION   DANLUQ010000223 DANLUQ010000000
VERSION     DANLUQ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.225"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(21..311)
                     /locus_tag="PXB27_002483"
     CDS             complement(21..311)
                     /locus_tag="PXB27_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347218.1"
                     /translation="MNNLSEFKLRYINNPYSNKDTFFIFDELSILLKSLTSDELKKIG
                     TAKNLADRQLNKQRKRCKRYVSEAYRLVEDNKEPYSKFFNHDGITAYNIKAF"
     gene            complement(361..1125)
                     /locus_tag="PXB27_002484"
     CDS             complement(361..1125)
                     /locus_tag="PXB27_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005704989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL5347219.1"
                     /translation="MPNITPKLVINALEPAKEKGIGDTLKRAIRLLNEVMNFAVNADV
                     IEFNKCQNVGSNFSVAKSEHNPTIHPKELPQFLAALRDYNLSFQTKMLIKWQLLTMTR
                     SREASETLWSEIDLEAKTWTISAERMKMKRPHVIPLSRQAVEILERMRKITGNSPFVF
                     QSEMKPRQPMNSQTANRAIGLLGYAGTLTAHGMRSIASTYLNEQLVNYDVVEACLAHV
                     IKDQTRKAYNRSDYLDQRVDVMQQWADYVESCSTGI"
     gene            complement(1094..1573)
                     /locus_tag="PXB27_002485"
     CDS             complement(1094..1573)
                     /locus_tag="PXB27_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HDL5347220.1"
                     /translation="MARVTTELNNTQIKNAKAQTKDYKLMDGKGLFLLVKKNGSKLWR
                     FRYKKPITGKESDLGIGSYPETTLAQARTIREEYRALLAENIDPLEHRQRLEAEALAE
                     IENTFYKVCLKWRDKCESRVKNGELQALTLKKNWRILEKYLLPCRLLYSKHHAKTSY"
BASE COUNT          560 a          394 c          374 g          709 t
ORIGIN      
        1 aaaaaggctc tctaaataga ttagaaagcc ttgatattgt atgcggttat cccatcgtgg
       61 ttgaaaaact tagagtaagg ttctttatta tcttcaacaa gacgataagc ctctgataca
      121 taccttttac atctttttct ttgcttatta agttgtctat ctgccagatt tttagctgtt
      181 cctattttct ttaattcatc ggaagtcagt gattttaata gtatgcttaa ttcatcaaat
      241 ataaaaaatg tatctttatt agaataaggg ttattgatat atcgtaattt aaactcagaa
      301 aggttattca tatttgtaca cctctaatta aacaagcggt caaattccgc caaaaattta
      361 ctaaatcccc gttgagcagc tttctacata atccgcccat tgctgcatta catctacacg
      421 ctgatctaaa taatcagaac ggttataagc ctttcgggtt tggtctttga taacgtgagc
      481 taaacaagcc tctactacat cataattgac taactgctca ttcaaatagg ttgaggcaat
      541 agaccgcata ccgtgagccg ttagagtgcc ggcatagcct aataagccta tggctctgtt
      601 ggcggtttgg ctgttcatcg gttggcgtgg tttcatttcg ctttgaaaaa caaatgggct
      661 attccctgtg atttttcgca ttctttctaa aatttcgacc gcttgccgtg aaagagggat
      721 aacgtgaggg cgtttcatct tcattctttc ggctgaaatg gtccacgttt tcgcctctaa
      781 atcaatttca ctccacaacg tttcgcttgc ctctcgtgag cgtgtcattg ttaatagttg
      841 ccatttaatc aacatcttag tttgaaatga taggttgtaa tcacgcaatg cagctaaaaa
      901 ttgaggtagc tcttttgggt gtatcgttgg gttatgttca gatttcgcta cgctaaaatt
      961 tgagcctaca ttttggcatt tgttaaactc gataacgtcc gcatttaccg caaaattcat
     1021 cacttcatta agtagcctaa ttgctcgttt aagggtgtcg cctatgcctt tttctttagc
     1081 aggctctaaa gcattaataa ctagttttgg cgtgatgttt ggaatagagt agtcggcaag
     1141 gcaaaagata cttttcaagg attcgccaat tcttcttcag cgttaaggct tgaagttcgc
     1201 cattttttac acggctttcg catttatcac gccatttcaa gcagacttta taaaaggtgt
     1261 tttctatttc ggctaacgct tcagcctcta atctttggcg gtgttcgagt gggtcgatat
     1321 tttccgctag taatgcccta tattcttctc taatcgttct tgcttgggct agggtagttt
     1381 cggggtaact gcctatgcct aaatcgctct ctttgccggt aatcggcttt ttatacctaa
     1441 accgccataa cttcgagccg tttttcttaa ccagcaaaaa cagccccttg ccgtccatta
     1501 gcttgtagtc tttggtttga gcctttgcgt ttttgatttg agtgttattt aattctgtgg
     1561 taactctagc cataaaaatt tacctaaaat cgcattgtgc tttatcagaa tcataaggga
     1621 tttaagggta tgggtttcat acccttttac gttataccct caaatatacc cttaaaataa
     1681 ggggatgtaa acgcaagaac acgaaagaat acgaatgaag aaataattta aaattgctct
     1741 aaagccagta tcatcaatgg ttttgcgtaa gaatgcgaat gaagaaataa gagtggaaaa
     1801 tttaagttgg tcccagcctc ctctgaactc gcttataact tattgattta taaagagata
     1861 aaataaactc tacaaaaata gggatcattt tagggatcat ttacataaaa gctatttatt
     1921 ttcgtttata aaatgttcat ttgtgcggct aggttggttt gtctttccta gttgcatttc
     1981 tattctatta tcagcaacta ttcagccctt cagcaagatt tcatttttca tacatca
//
LOCUS       DANLUQ010000224         2031 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.226, whole genome shotgun sequence.
ACCESSION   DANLUQ010000224 DANLUQ010000000
VERSION     DANLUQ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.226"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            83..2014
                     /locus_tag="PXB27_002486"
     CDS             83..2014
                     /locus_tag="PXB27_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL5347221.1"
                     /translation="MIFFTDLILKRGHSVLIDNSSVTIHTGQKVGLVGKNGCGKSSLF
                     ALLKNELQPEGGDVSLPKNWAISWVNQETPALAISALDYVIQGDREYTHLMAELDIAN
                     EKNDGNRIAIIHTQLDTIDAWTIQSRAATLLNGLGFSTEQLQLPVKSFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLERWLSNYRGTLLLISHDRDFLDPIIDRVIH
                     IEHQKLNEYTGNYTSFEIQRATKIAQQNAAYQQQQRKVAHLQSFIDRFKAKATKAKQA
                     QSRIKALEKMELIAPAYADSPFEFEFRPPLALPSPLLMMEKVSAGYAEKTVLESVKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELPPQNGSIQLAKGVQLGYFAQHQVDTLRFDE
                     SPLWHLQKLAPEKTEQELRNYLGGFDFKGDKVVQAVKSFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLEMRQALVDALTYYEGSLVVVSHDRHLLRSTVNEFYLVHDRKVEKF
                     KGDLDDYQKWLNEQNALANEPKSVLAKDSANSTACSSTNENSAAQRKEQKRLEAERRQ
                     QTAPLRKKITQLEKELEKATEKLNKLEAMLTESDIYEAENKAKLTETLAKQLEAKRLV
                     EEIEMQWLEAQEEFETMLR"
BASE COUNT          619 a          441 c          456 g          515 t
ORIGIN      
        1 ttcttatttt agttcacaaa gcaatggctt tgctttttta tgctaaaata cgcaaaattt
       61 ttaacaaaat agaccgcttg caatgatctt ttttacagac ttaattttaa aacgagggca
      121 ttccgttctc attgataaca gctccgttac cattcacacc gggcagaaag tcggtttggt
      181 gggaaaaaac ggctgtggca aatcctctct ttttgcctta ctgaaaaatg aattacaacc
      241 tgaaggtggc gatgtatcgt tgccgaaaaa ctgggcgatc tcgtgggtaa atcaagaaac
      301 gcctgcgtta gctatttcag cgttagacta tgtgattcaa ggagatcggg aatataccca
      361 tcttatggct gagctagata tcgcaaatga aaagaatgac ggcaaccgca ttgcgattat
      421 ccatactcaa cttgatacca ttgatgcttg gacaattcaa tcccgtgccg ctaccctgct
      481 taacggcttg gggtttagca ccgaacaact gcaactgccg gtgaaatcct tctccggtgg
      541 ctggcgtatg cggttaaatt tggcacaagc tctgatttgt cgttccgatc tgttattgct
      601 tgacgagcca accaaccact tagacttaga tgcggttatt tggttggagc gttggctgag
      661 taactatcgt ggcactttat tgttaatttc gcacgaccgt gattttctcg atccgattat
      721 cgatcgggtg attcatattg aacatcaaaa gctcaatgaa tacaccggca attacacctc
      781 gtttgagatt caacgtgcga ccaaaatcgc ccagcagaat gccgcttatc agcaacagca
      841 acgtaaagtt gctcatttgc agagttttat cgatcgcttt aaagccaaag cgactaaagc
      901 gaaacaggca caaagccgaa ttaaagcgtt ggaaaaaatg gagcttattg ccccagccta
      961 tgctgattcg ccgtttgaat ttgaatttcg cccaccgctt gcactgccta gcccgttatt
     1021 aatgatggaa aaagtcagtg ccggctatgc ggaaaaaacg gtgttggaat cggtcaaact
     1081 gaatttggtt cccggctctc gtattggttt actcgggaga aacggtgcag ggaaatccac
     1141 gctgattaaa ttattggcgg gcgaacttcc gccccaaaac ggctcaattc aactggcaaa
     1201 aggcgtgcaa ctcggctatt tcgcccaaca tcaggtggat acactacgtt ttgatgaaag
     1261 cccactttgg catttacaaa aactcgcacc ggaaaaaacc gagcaggaat tacgcaatta
     1321 tttaggcggt ttcgatttta aaggcgataa agtggtacaa gctgtgaaat cattctccgg
     1381 cggagaaaag gcccgtttag tgcttgcctt aattgtatgg caacgcccga atttactgct
     1441 attagatgag ccaaccaacc atttagattt agaaatgcga caagccctgg ttgatgcact
     1501 gacctattac gaaggctcgc tagtggtggt ttcgcacgac cgccatttat tgcgtagcac
     1561 cgtgaatgaa ttttatttag tgcacgaccg caaagtggaa aaatttaaag gtgatttaga
     1621 cgattaccaa aaatggctca atgagcaaaa cgccttagcc aacgagccga aatccgtact
     1681 tgcaaaagat tcggcaaatt cgaccgcttg ctctagcacc aatgaaaata gtgcagctca
     1741 acgcaaagag caaaaacgct tagaggcaga gcgccgccaa caaaccgccc cattgcggaa
     1801 aaaaattact caactggaaa aagagttaga aaaagcaacc gaaaaattaa ataaactcga
     1861 agcaatgctt accgaaagcg acatttatga agccgaaaat aaagcaaaac taactgaaac
     1921 acttgcgaaa cagttagagg caaaaaggct ggttgaagaa attgaaatgc aatggttgga
     1981 ggctcaggag gagtttgaga ccatgttacg ctagggttct acaagatagc g
//
LOCUS       DANLUQ010000225         2001 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.227, whole genome shotgun sequence.
ACCESSION   DANLUQ010000225 DANLUQ010000000
VERSION     DANLUQ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.227"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            124..807
                     /locus_tag="PXB27_002487"
     CDS             124..807
                     /locus_tag="PXB27_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDL5347222.1"
                     /translation="MFGLFASILTGARGGWLAFIPICFFILFLNLRQLNKKAIFLILL
                     ALISSLSFFISKPEFGIQKRYNAAKNDIIVFLEKGNRHSSLGARFDMWENALIGIREK
                     PLLGHGSTGYETFKKKQIETKQMAKTTLRFNSLHNQYLEAFVKRGVVGFMALMAILII
                     PFVIFAKRLKAKNREVKCMAILGIVHIISHSFFFLSQSFLAHNSGSLFYFFVLILLYH
                     LVKQNEEII"
     gene            complement(909..1916)
                     /gene="rfbB"
                     /locus_tag="PXB27_002488"
     CDS             complement(909..1916)
                     /gene="rfbB"
                     /locus_tag="PXB27_002488"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDL5347223.1"
                     /translation="MIILITGGAGFIGSALVRYLIEHTPHTVINVDKLTYAGNLASLE
                     SVAHHERYHFEKADICASGQISEIFGKYQPDVVMHLAAESHVDRSISGAADFIQTNIV
                     GTYTLLEAARAYWDTLPEPRKSAFRFLHISTDEVYGDLAKSEELFTEKTPYNPSSPYS
                     ASKAASDHLARAWHRTYGLPTVITNCSNNYGAYQYPEKLIPLMILNALEGKSLPVYGN
                     GLQVRDWLFVEDHIRALYLVLQKGQIGETYNIGGHNEKTNIEVVRSICSILQEMVPNN
                     PLVVGQYADLITYVQDRPGHDFRYAIDTSKIEKELDWKPKETFESGLRKTVEWYLANR
                     N"
BASE COUNT          607 a          383 c          380 g          631 t
ORIGIN      
        1 gaaataatgc gtattcaatc ggggaacata gcaatattat tatcactatt tagtatagca
       61 attgcctttt attggctttc aaaaaaaaga attaaaactg gcattgccta cctggttttt
      121 gccatgtttg ggttatttgc cagtattcta acaggagcaa gaggcggctg gcttgcattc
      181 attcccattt gcttttttat cctattttta aatttaagac agctaaataa gaaagctatt
      241 ttcctgattc tattagcact tatatcttca ctctcatttt ttatctccaa accagaattt
      301 ggcatacaaa aacgatataa tgcagctaag aatgatatta tcgtattttt agaaaaagga
      361 aatagacatt catctctggg agctcgtttt gatatgtggg aaaatgcgct tatcggaatc
      421 cgtgaaaaac cgctattagg acacggtagc acaggctatg aaacattcaa aaagaaacag
      481 attgaaacaa aacaaatggc aaaaacaacg cttaggttta atagcttaca caaccaatac
      541 ctagaagcct ttgtgaaaag aggtgtagtt ggttttatgg cgctaatggc tattttaatt
      601 attccatttg ttatttttgc caaacgatta aaggcaaaaa atagagaggt aaaatgtatg
      661 gcgatattag gcattgtaca tattatttcg cattcattct ttttcctaag ccaatcattt
      721 ttagcccata attcaggctc attattctat ttctttgtgt taattttact ctaccattta
      781 gtcaaacaga acgaagagat aatatgataa aacaatagaa gaaagttggg aatataaccg
      841 aggtggtaac ttcatcttct accacaggat aaatgtcggg atatttcctc tttttctgct
      901 tcttactact aattccgatt agccaaatac cattccactg ttttacgcaa gccactttca
      961 aaagtttctt tcggtttcca gtcgagttcc ttttcgattt ttgaagtatc aattgcatag
     1021 cggaaatcgt ggccagggcg atcttgcaca taagtaatta aatcagcata ttgtcctaca
     1081 acaagcgggt tatttggcac catttcttgc aaaatagaac agatagaacg aaccacttcg
     1141 atattggttt tttcattatg cccgccaata ttgtaggttt ctccgatttg ccctttttgc
     1201 aacaccaaat agagggcacg aatatgatct tccacaaaca gccaatctct gacctgcaag
     1261 ccgttgccgt aaacaggtag ggatttacct tctaaggcat ttaaaatcat taacggaata
     1321 agtttttcgg gatattggta tgccccataa ttattggaac agttggtgat gacagtcggt
     1381 aaaccgtagg tgcgatgcca agcccgagcc agatgatcgc tggcagcttt agatgccgaa
     1441 taggggctac ttggattata aggggttttc tcggtaaata attcctcgct tttggctaaa
     1501 tcaccataga cctcgtcggt cgagatatgt agaaagcgaa acgccgattt ccttggctca
     1561 ggtaacgtgt cccaataggc tctcgctgcc tctaataaag tataagtgcc gacaatattg
     1621 gtttgaataa aatctgctgc cccagaaata gagcgatcaa catggctttc cgctgctaag
     1681 tgcattacca catcaggctg atatttgcca aaaatctcag aaatttgacc gcttgcacaa
     1741 atatctgctt tttcaaaatg gtagcgttcg tgatgtgcga cactctccaa tgaggctaaa
     1801 tttcccgcat aggtgagctt atccacattg ataactgtgt gtggagtatg ttcaatcaga
     1861 tagcgaacta atgcagatcc gataaaaccg gctccgccag tgatgaggat gatcataaag
     1921 gtgaatatga aatttgattc acgaaatttc gtttgtattt aatatggctt tgaatattct
     1981 tttcagcgtc ttttttatcc c
//
LOCUS       DANLUQ010000226         1997 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.228, whole genome shotgun sequence.
ACCESSION   DANLUQ010000226 DANLUQ010000000
VERSION     DANLUQ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.228"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..1213)
                     /locus_tag="PXB27_002489"
     CDS             complement(<1..1213)
                     /locus_tag="PXB27_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDL5347224.1"
                     /translation="MSIQQKKRHSRWYERIFFSTAVQSILGVMFSSVLPAIYFWGEKF
                     NSIETRSNTIIATSLAFVMIAISLRVLMKYPGNKSASFVFSTVISWYAVLLIVLTIFR
                     FDYSNTLLGTSFILTILFCFIGYFLSRSWIIPKIALVPFGRAINLNRIQNVEWIQLSE
                     PILGEKRINSIAVDLHYPDLSAEWHKFLAKCTLNGIPVYNVRQLEESLTGRVKIRHMY
                     ENDLGSLLPSPIYSLVKRIIDIALIVATFPITLPIMTITAIAIRLESEGGAMFIQKRV
                     GQGGKEFNIYKFRSMCKDSEKDDAQFASSADMRVTRIGKFIRKTRIDELPQFFNVLKG
                     DMSLIGPRPEQTAFVDKFEEEIPFYNYRHIVKPGISGWAQVVQGYAADVDDTKVKVEH
                     DFYYIKHFSLWL"
     gene            complement(1217..1997)
                     /locus_tag="PXB27_002490"
                     /pseudo
     CDS             complement(1217..1997)
                     /locus_tag="PXB27_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_162680708.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT          688 a          400 c          298 g          611 t
ORIGIN      
        1 caagccataa agaaaaatgc ttaatatagt agaagtcatg ttctactttt acttttgtat
       61 catcaacatc tgccgcataa ccttggacga cctgagccca accggaaata cccggtttaa
      121 cgatatggcg ataattatag aatggaattt cctcttcaaa tttatctaca aaggctgtct
      181 gttctgggcg aggaccaatt aagctcatat caccttttaa tacattaaaa aactgaggta
      241 gctcatcaat acgagtttta cggataaatt tcccaatacg agttaccctc atatccgcac
      301 tcgaagcaaa ctgtgcatca tctttttcgg agtctttaca catactacga aatttataaa
      361 tattaaactc ctttccacct tgcccaacgc gtttttgaat aaacattgct ccaccttcac
      421 tttctaagcg aatagcaata gcggtgattg tcataatagg taaagtaatg ggaaacgtag
      481 caacaatcaa tgcaatatca atgatacgct tcactaatga gtaaattgga gaaggaagta
      541 atgaacctaa gtcattttca tacatatggc gaattttgac acgcccggtt aaagattcct
      601 caagctggcg aacgttatat acaggaatac cattcaatgt acatttcgct aaaaatttat
      661 gccattctgc ggacaaatca ggatagtgta aatctaccgc aatagagtta attctctttt
      721 cccccaatat tggttcactt aattgaatcc actctacatt ctgaattcgg ttcaaattga
      781 tagctcttcc gaatggaacc aaagcgattt tagggataat ccaacttctt gaaagaaaat
      841 aaccaataaa acaaaataga attgtgagta taaagctcgt acctagcaat gtattagaat
      901 aatcaaatct aaatattgtt aacacgatta ataaaaccgc ataccaagaa ataacggtgg
      961 aaaaaacaaa acttgctgac ttattacctg gatatttcat taacactctt aagctgatag
     1021 ctatcattac aaaggcaaga gaagtcgcta taatcgtatt agaacgagtt tcaatagaat
     1081 taaatttctc tccccaaaaa tagatagcgg gtaagacact tgagaacata acgcccaaaa
     1141 tactttgaac agctgtacta aaaaagatac gttcatacca acgagagtgt cttttctttt
     1201 gctgaatgct cataatttat ccttttctta atttcttata aagtcttcct aaaagagatc
     1261 taggcaataa ccgtggtaag gctctaagta taaaaaacat gcttgatgag attattgatt
     1321 gtaatcccaa ctcttttttc aattttgcta attggtactc actcttaatg taattccacc
     1381 ctttacgccg agcgtacatt gcagaacctg aacgaacatt taccagtaca tctggtaaat
     1441 tatacacctc atatttctgt gtaataactc gtaaccataa attgtaatct tccatataaa
     1501 gatgatgctg atagcctcca acatgctcga tcacagattt tttataggct actgccatat
     1561 ggttaaaagg atttcttagt aatgctgaaa tacgaatttc atcatcatta atgggcactt
     1621 gttttatacc cagagaatta gacattgttt cgtcaaattc ttcaacttgt ccccccaata
     1681 aaaccacatc aggattttta ctaataaaat caatttgctt ttcaaatcga gatggtaagc
     1741 aaatatcatc agtatccatt ctaaacaccc actcattaga gcagtgtttt aagccttcat
     1801 ttaaggccgg cctaaaccca cattttgtgg taaactaaca acaacaagcg gtaatttttc
     1861 agtaaaatgt tgcacaattc gctctaaatc tccaggaatt ataccatcaa aaaccacaac
     1921 aatttcactt gctggtaaag tttgggatac taaagaatct aaacacatct ctaaataagc
     1981 tggtttttct ttataat
//
LOCUS       DANLUQ010000227         1989 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.229, whole genome shotgun sequence.
ACCESSION   DANLUQ010000227 DANLUQ010000000
VERSION     DANLUQ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.229"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            259..957
                     /gene="aphA"
                     /locus_tag="PXB27_002491"
     CDS             259..957
                     /gene="aphA"
                     /locus_tag="PXB27_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HDL5347225.1"
                     /translation="MTAATMFASLIAFSAQAGGPKVAYTQPGIDMRTHEAQQAPIHWV
                     SIEQIKESIKDLPPMAVSFDIDDTVAATSGCFHYGKQKYSPDDFSYLKNQDFWDEINA
                     GCDKYSLPKQVAINLIKMHQERGDQVYLITGRTAGKNEQVTTIMQKYLGIKNMQPVNF
                     MGGKAHSTKYDKTPAIIEHKVQIHYGDSDDDILAAREAGIRGIRILRAANSNYTPFPQ
                     AGGYGEEVVVNSSY"
     gene            1138..1899
                     /gene="udp"
                     /locus_tag="PXB27_002492"
     CDS             1138..1899
                     /gene="udp"
                     /locus_tag="PXB27_002492"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HDL5347226.1"
                     /translation="MSEVFHLGLTKAMLKGAKIAIVPGDPARSERIAKKMDNPEFLAS
                     TREFTSWLGYLDGEPVVVCSTGIGGPSVSICVEELAQLGVRTFLRIGTTGAIQEHINV
                     GDILITTGAVRLDGASQHFAPMEYPAVANFECTNALYAAAVESGVTPYVGITASSDTF
                     YPGQERYDTFTGKIYRHFQGSLKQWQDLNVMNFEMESATLFTMCSALGLRAGMVAGVI
                     VNRTQQEIPNEATIHDTEHKAVSVVVEAARKLIKG"
BASE COUNT          629 a          347 c          426 g          587 t
ORIGIN      
        1 ggagccattt ttattaagcg aatatgatgc ctatcaattt acgaatgagg tgattaaatt
       61 tcattcagaa gaaagcctta aagcttgggg aggttgcttg atttgggaac agaacaatgg
      121 ctcataagcg ttaagcgtaa tcgtttgctt gtgatcatat tcacaaaata aggtttttgt
      181 attagctaag agtgcctaat aagattaaaa taggtatggt taatcatatg gaggtatttt
      241 ataaaattta cattaaaaat gacggcagcg acaatgtttg catctcttat tgctttttca
      301 gcacaagcag gcggaccaaa agtagcttat acccagccgg gaattgatat gcgaacacat
      361 gaagcacagc aagcgccaat tcattgggta tctattgagc aaattaaaga aagcatcaaa
      421 gatttacccc ctatggcggt aagttttgat attgatgata ctgttgctgc aactagcggt
      481 tgcttccact acggaaagca aaaatattcg ccagacgatt tcagctactt aaaaaatcaa
      541 gatttttggg acgaaattaa tgctggctgt gataaatatt ctttgccaaa gcaagttgct
      601 attaatttaa taaaaatgca ccaagaacgt ggtgatcaag tttatttaat tacggggcgt
      661 accgcaggta aaaatgaaca agttacaacg attatgcaaa agtatcttgg tattaaaaat
      721 atgcaaccgg taaattttat gggggggaaa gcacattcaa caaaatatga taaaacccct
      781 gcgattattg aacataaggt gcaaattcac tatggtgaca gtgatgatga tatcttagct
      841 gcccgtgaag ccggtattcg aggtattcgt atacttcgtg cagcaaattc taattatacg
      901 ccattccctc aggccggagg atatggtgaa gaagttgttg tcaattcaag ttattaattt
      961 cttagtctga aatatggcca ccattgcgtg gccatcattt tttatttata taatgacgaa
     1021 accgtttacc tttgatctag ttcacaaaat caaactttgc ttgcaaaaaa ttgtcgtaaa
     1081 ttaaccgctt gctaagatag aatggctcat atccagttag ataaaaagga gataaaaatg
     1141 tctgaagtat ttcatttagg tttaaccaaa gccatgctca aaggtgcaaa aattgcaatc
     1201 gttccgggcg atccggctag aagtgaacgt attgccaaaa aaatggacaa cccagagttt
     1261 ttagcctcaa cccgtgaatt tacctcttgg ttaggttatt tagatggcga accggtggtg
     1321 gtttgttcaa ccggtattgg cggaccgtcg gtgtcgattt gtgtggaaga gttagcacag
     1381 ttaggtgttc gcacattctt acgcattggc acaacaggtg caattcaaga gcatattaat
     1441 gtaggcgata ttttaattac caccggagcg gtgcgtttag atggtgcgag ccaacatttt
     1501 gcaccaatgg aatatcctgc agtagcgaat tttgaatgta ctaatgctct ttatgcagca
     1561 gcagtggaat caggcgttac accttatgtg gggattacag cttcatcaga cactttctac
     1621 ccggggcaag aacgttacga cactttcacc ggcaagatct atcgtcattt ccaaggttca
     1681 ttaaaacagt ggcaagatct caatgttatg aattttgaaa tggaatccgc cactctcttt
     1741 acgatgtgtt cggcattagg cttgcgagca ggtatggttg ctggtgtaat tgtaaaccgc
     1801 actcaacaag agattccgaa tgaagcaacc attcacgaca ccgaacataa agccgtttcg
     1861 gtagtagtgg aagcggcaag aaaattaatt aaaggctaat ctttaaattt aaaagcaaaa
     1921 aaggtaaatt gtctttgtta caatttacct tttttctttt tagggagctt tctatgaaaa
     1981 aatggttga
//
LOCUS       DANLUQ010000228         1923 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.230, whole genome shotgun sequence.
ACCESSION   DANLUQ010000228 DANLUQ010000000
VERSION     DANLUQ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.230"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..929
                     /locus_tag="PXB27_002493"
     CDS             <1..929
                     /locus_tag="PXB27_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005693550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferasee"
                     /protein_id="HDL5347227.1"
                     /translation="QLPPFQTQFLHPKYWGLWLGLGVFKLILCLPYPILVKIGLGLGK
                     LFTKLGFGKRRMRIARRNLELCFPHYSAEKVEELVEENMKSVGMAIIETGMAWFWSDK
                     RILKWSKIEGLEHLTQRDQERGILLVGVHFLTLELGARIIGLHHQGVGVYRPNDNPLL
                     DWIQFRGRVRSNKAMLDRKDVRGMIRALKNGEMIWYAPDHDYGRRNSVFVPFFAVEET
                     CTTAGSYMLLRSAPKSLVIPFSPIRNLDYSGYTVKVSPAVDFSQCQNEVETAILMNRV
                     VEREIMAALTQYMWLHRRFKTRPNEGDQSLYD"
     gene            1025..1912
                     /gene="dapA"
                     /locus_tag="PXB27_002494"
     CDS             1025..1912
                     /gene="dapA"
                     /locus_tag="PXB27_002494"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDL5347228.1"
                     /translation="MATPLFHGSIVALVTPMTHGEVDYQDLKKLVEYHIQAGSHAIVS
                     VGTTGESTTLSIDENVKVIKKTVEFADGRIPIIAGTGSNATSEAITMTKLLANSGVAG
                     CLSVVPYYNKPTQEGMYLHYKAIAESTDLPQILYNVPGRTGSDLKPETIGRLAKIENI
                     VAVKEATGDLSRLPLIKQLAGEDFIFLSGDDATGFESMKLGGQGVISVTNNVAAADMA
                     KMCELALAGQFTEAEEINNRLMALHHDLFVESNPIPVKWAAYKLGLISEPNLRLPLTT
                     LSESAQPVVLKALQQAGLI"
BASE COUNT          578 a          305 c          435 g          605 t
ORIGIN      
        1 aacagttacc tccattccag acacaatttt tacaccctaa atattgggga ttgtggctcg
       61 gtttaggtgt ttttaagtta attttatgtt tgccttatcc tattttagta aaaattggtc
      121 tgggactagg taaattgttt actaaattgg gttttggtaa acgtcgtatg cgtattgctc
      181 gccgtaattt agagctctgt tttccccatt actctgctga gaaagtagag gaattagtcg
      241 aagaaaatat gaaatcggtt ggaatggcga ttattgaaac tggtatggct tggttttggt
      301 cggataaacg tattcttaaa tggtctaaaa tcgaagggct agagcattta acacagcgtg
      361 atcaggaaag gggtatttta cttgtagggg tgcattttct gactttagaa ctcggtgcaa
      421 gaattattgg gctacatcat caaggagtag gggtttatcg accgaatgat aatccattat
      481 tagattggat acagtttcga ggacgtgttc gctcaaataa agcgatgtta gatcgtaaag
      541 atgtgcgtgg aatgattcga gctttaaaaa atggcgagat gatatggtac gcgcctgatc
      601 acgattatgg aagacgaaat agcgtttttg taccattttt tgcagtggag gaaacttgta
      661 ctactgcggg tagttatatg ttacttcgtt ctgccccaaa gagtttagtg atcccgttta
      721 gcccgattcg aaatttggac tattcaggct acacagtaaa agtcagtcct gccgttgatt
      781 ttagtcaatg tcaaaatgaa gttgaaacgg ctattttaat gaatcgggtg gtagaacgtg
      841 aaattatggc tgcgctaact caatatatgt ggctacatcg ccgttttaaa actcgcccaa
      901 atgaaggtga tcaaagccta tatgattaat tttaaataat ttcaaaaaag tgcttgcata
      961 tttaagctga attttgcaat ctagcaatgt tatcaagcat aataaaaaaa tacaggagat
     1021 gattatggcg acccctttat ttcacggtag tatcgtagca ttagttacac caatgactca
     1081 tggtgaggta gattatcagg atttaaaaaa attagttgag tatcatattc aagcaggttc
     1141 tcacgctatt gtatctgttg gtacaacagg tgaatctacc acattaagca ttgatgaaaa
     1201 tgttaaggtg attaagaaaa cggttgaatt tgctgatgga cgtattccaa ttattgcagg
     1261 aactggttct aatgcgacca gtgaagctat cactatgact aaattattag caaacagtgg
     1321 tgtagctggt tgtttaagtg tagtgcctta ttacaacaag ccaacgcaag aggggatgta
     1381 cttacactac aaagcgattg cagaaagtac agatttacca caaattctgt ataatgtgcc
     1441 ggggcgaaca gggagtgatt taaaacctga aacaatcggt cgcttagcga aaattgagaa
     1501 tattgttgct gtgaaagaag caacgggtga tttaagccgc ttacctttaa ttaagcaatt
     1561 agccggtgaa gattttattt tcttaagtgg tgatgatgct accggttttg agtctatgaa
     1621 attaggtggt caaggggtga tctcggtcac caataatgtt gctgctgctg atatggcaaa
     1681 aatgtgtgag ttagctcttg ccggtcaatt tactgaagca gaagaaatta ataaccgctt
     1741 aatggcgtta caccatgact tatttgtcga atctaacccg attccggtga aatgggctgc
     1801 ttataaatta ggtttaatta gtgagcctaa tttacgttta ccgcttacca cactgtctga
     1861 atcagcacag cctgtagtgt taaaagcatt acaacaagcg ggcttaattt agttattttt
     1921 tgc
//
LOCUS       DANLUQ010000229         1920 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.231, whole genome shotgun sequence.
ACCESSION   DANLUQ010000229 DANLUQ010000000
VERSION     DANLUQ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.231"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..272)
                     /locus_tag="PXB27_002495"
     CDS             complement(<1..272)
                     /locus_tag="PXB27_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347229.1"
                     /translation="MININTIKYSEIFNLRLKRKEKNVPWHIVPKDGTKAFCKQIGAS
                     AVEILREFPTLSDINLNNLPDSFVLKPENGHSDGGVMLLKKIEDNL"
     gene            complement(282..>1920)
                     /locus_tag="PXB27_002496"
     CDS             complement(282..>1920)
                     /locus_tag="PXB27_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL5347230.1"
                     /translation="EFKLFPLNKANFESQIKGSLSLGLFIESCWKGNFGAWEYAFTSP
                     NLQHQNAQNLLKALDVCKNRNFPIVFWNKEDPMHYEKFLPISSKCDVIFTTDSNKVKD
                     YQRDVPNAKVETLMFAANINICNPANRFRYEAENICFAGSYYGVGHDDRKKQMDALLP
                     TIIKFKGAIYDRMSQVQSADERYSYPKQYRKFIRPAVPFKEIVDIYKQFKIFLNVNTI
                     TDSPTMMSRRVYELLACGTPVISTPSLAIDEQFKGIVQVAKDAKEANKIAKRLLENEW
                     EWLRISHLGYREVMLKHTYEHRAVQIANTLGQDIKPEAPLASIVVASNRPYFIDRIVD
                     NVTNQSYPNIEVIVIAQKYTAEQLDNLKQKLSKSNKLKNVIVVQNDSDDTLGKRLNQG
                     IKLSKGEYVAKFDDDDFYFPNYLQDMLIPFKFGDYGIIGKKEIFIFLESQNKTFVRYK
                     GQRHLETDFLTGATLVFARDALKKLSFGDLNRGEDSNILEQAKKLGIKMYVTDPFNFV
                     VFRSKELSNHTWQVDDNFFTNKGVFVGEELAKDITKL"
BASE COUNT          616 a          342 c          269 g          693 t
ORIGIN      
        1 tagaggttat cctcaatttt ctttaaaagc attaccccgc catctgaatg gccattttca
       61 ggttttaata caaaactatc tggcagatta ttcagattta tgtcagataa agttggaaat
      121 tccctgagaa tttcgacagc actagcacca atttgtttac aaaatgcctt tgttccatct
      181 tttggtacga tatgccaagg tacatttttt tcttttcttt ttaaacgtaa attaaaaatt
      241 tcagagtatt taattgtatt tatatttatc atctttgttt cctataattt tgtaatgtct
      301 ttagctaatt cttcgccaac aaaaacacct ttattagtga agaaattatc atcaacttgc
      361 caagtatgat tagaaagttc ttttgaacgg aaaacgacaa aattgaatgg gtcggtaaca
      421 tacattttga tacctaattt tttcgcttgt tctagaatat ttgaatcttc tccacgattt
      481 aagtctccaa atgatagttt ctttaaggca tctcttgcga atactaaagt tgctcctgtt
      541 aagaaatcag tttccaaatg tcgttgtcct ttataacgaa caaaagtttt attttgactt
      601 tctaaaaaaa taaagatttc ttttttacct ataatgccat aatcaccaaa cttaaatgga
      661 ataagcatat cctgcaaata atttgggaaa taaaagtcat catcatcaaa ttttgctaca
      721 tattcccctt tggataattt aattccttgg ttcaatcgtt tacccaaagt atcatcactg
      781 tcattttgaa caacaatcac atttttcagt ttattactct tggagagctt ctgttttaag
      841 ttatctaatt gttcagcagt gtatttttga gcaatgacga tcacttcaat atttggatag
      901 ctttgatttg taacattatc aacaattcta tctataaaat atggtcgatt agatgcaaca
      961 acaatacttg ccaatggggc ttcaggctta atatcctgcc ctaatgtatt tgctatttgt
     1021 acagctctat gctcataagt atgttttaac attacctctc gatagcctaa atgagaaatt
     1081 cttagccact cccattcatt ttctaacaat ctcttagcaa ttttattggc ttctttggca
     1141 tctttagcta cttgtacaat acctttaaat tgttcatcta ttgctaatga tggtgtagaa
     1201 attacaggtg taccgcatgc caataattca tacactctgc gtgacatcat tgtaggagaa
     1261 tcggtaatag tattaacatt taaaaatatt ttaaattgtt tataaatatc aactatttcc
     1321 ttaaatggga ctgccggtct gataaatttg cggtattgtt taggataaga ataacgctca
     1381 tcagctgact gtacctgaga catacggtca taaatagctc ctttaaattt gataatagta
     1441 ggcaaaagag catccatttg cttcttacga tcatcatgac caacaccata ataagaacca
     1501 gcaaaacaga tattttcagc ttcatatcgg aaacggtttg ctggattaca aatattgata
     1561 tttgcagcaa acattaatgt ttccacttta gcattaggaa catctctttg ataatctttc
     1621 actttgttgc tatctgtagt aaaaatcaca tcgcatttag atgaaattgg taaaaacttc
     1681 tcataatgca tcggatcttc tttattccaa aatacaatag gaaaatttct atttttgcaa
     1741 acatcaagag cttttagtag attttgagca ttttgatgct gtaaatttgg cgaagtaaag
     1801 gcatactccc aagcaccaaa attccctttc caacaactct caataaacaa ccctaatgat
     1861 aaagaacctt taatttgact ctcaaaattt gctttattta acggaaagag tttaaattcg
//
LOCUS       DANLUQ010000230         1868 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.232, whole genome shotgun sequence.
ACCESSION   DANLUQ010000230 DANLUQ010000000
VERSION     DANLUQ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.232"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(344..1585)
                     /locus_tag="PXB27_002497"
     CDS             complement(344..1585)
                     /locus_tag="PXB27_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HDL5347231.1"
                     /translation="MMNKRIYVFADWETFSQPELVGVLSSDVVRGKENFRFSYDNEWL
                     SSPYVQQIDPNLYLYPGEQYSTDTQNFHIFLDSCPDRWGRLLMKRREAALASQENRKP
                     KLLIESDYLLGVHDMNRMGALRFKTELNGPFLDNNTRLAAPPLSSLRELEFAAIQLES
                     SDNVDDPDYLKWLHMLISPGSSLGGARPKASVIDELNQLWIAKFPSRYDDYDIALWEY
                     LAYRLAIQAKIEMATCRIERFNQEHHTFLTQRFDRHCGKRRHFTSALTQLGYYDGDYE
                     ASYLEIAQFLTESGSNTRIDLAQLWRRIVFNIAISNTDDHLRNHGFIYAQEGWILSPA
                     YDLNPVPLSQGLHLNITDSDNRLDYQLAFDVADFFQLSKQQAVQIYDEVLKEINNWQN
                     VAKEIGIPRQEQEQMKFAFNV"
     gene            complement(1585..1788)
                     /locus_tag="PXB27_002498"
     CDS             complement(1585..1788)
                     /locus_tag="PXB27_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL5347232.1"
                     /translation="MFTQTLLAQRTGLSRVTVRKIMNGDPTVAIGYYLMVLGVLGLEQ
                     DFTDVGKDDELGRKLQDIELLRK"
BASE COUNT          545 a          377 c          328 g          618 t
ORIGIN      
        1 aaatgttagt ttttctgatg tctgccatct gctaggcatt atatatgcct agattagttt
       61 cctacatttg cgtttttaag gctacgcttc cttgcggata tggttgagaa aaaagtcaag
      121 tgttactaat ggttttataa gatgttggtt tgttctggtg tctgccaccg ctaggtcttt
      181 ataccctaga ttagtatcag gtctttctgg tgcctgccac cgctaggcct ttacatccta
      241 gattaatatc agattgacaa tgagccaaca actcattgcg ttattaaggt aacgctactt
      301 ttttagaaca tagactacga tgattggttc cacaagtcaa ttactataca ttaaatgcaa
      361 atttcatctg ctcttgctct tggcgtggaa taccaatttc ttttgcgaca ttttgccaat
      421 tattaatttc tttcagtact tcatcataaa tctgtacggc ttgttgctta gataattgaa
      481 aaaagtcagc aacatcaaag gcgagctgat aatccaatcg gttatcgctg tctgttatgt
      541 ttaaatgtaa tccttgagat agtggtactg ggtttaaatc gtatgcggga gataatatcc
      601 acccctcttg agcgtagata aatccgtggt tacgcaaatg atcatcagta ttcgaaatag
      661 caatattgaa tacaattctt ctccatagtt gagccaagtc aattcgagta tttgaaccac
      721 tttctgttaa aaattgagca atctcaaggt agcttgcctc atagtctccg tcataataac
      781 ccagctgggt taatgctgac gtaaaatggc gacgttttcc acaatgtcga tcaaatcttt
      841 gtgtcaaaaa agtatggtgt tcttggttaa agcgttcaat tctacaagta gccatttcaa
      901 ttttggcttg aatagccagt cgataagcta aatattccca aagagcaata tcataatcat
      961 cgtaccgact tggaaatttg gcaatccata gttgatttag ttcatctatt accgaagctt
     1021 ttggtctagc tccgcctagt gacgaccctg gtgatatcaa catatgaagc cattttaaat
     1081 aatccggatc gtccacatta tcactgcttt caagttgtat tgctgcaaat tctaattctc
     1141 ttaaggaaga cagtggtggt gctgcaagac gagtattatt gtccaaaaat ggaccattca
     1201 attcagtttt aaaacgtaaa gcccccattc tattcatatc gtgtacgcct aaaaggtaat
     1261 cactttctat tagtaactta ggtttacgat tctcttgact agctagagct gcctcccgac
     1321 gtttcattaa caatctaccc catctatcag ggcaagaatc taaaaagata tgaaaatttt
     1381 gtgtatctgt actgtattgc tctccagggt aaaggtataa gttaggatca atctgctgta
     1441 catagggtga acttaaccac tcgttatcgt agctaaaacg gaaattttct ttgcctctca
     1501 ctacatcaga agaaagcacc ccaactaatt caggctgtga aaaagtttcc caatctgcaa
     1561 acacataaat tcgtttattc atcattattt ccttaataat tcaatatctt gcaatttacg
     1621 tcctaactca tcatcctttc caacatcagt gaaatcttgt tctagaccga gaacacctaa
     1681 aaccattagg tagtatccaa tcgcaacagt tggatcacca ttcataattt ttctcactgt
     1741 tacacggcta agcccagttc tttgagccaa taatgtttga gtaaacattc tcctttttga
     1801 ggctagccta atattttctc caagttgaag caaggcattt tggtacttag gaaacagagt
     1861 tggtgatt
//
LOCUS       DANLUQ010000231         1864 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.233, whole genome shotgun sequence.
ACCESSION   DANLUQ010000231 DANLUQ010000000
VERSION     DANLUQ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.233"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            110..691
                     /locus_tag="PXB27_002499"
                     /pseudo
     CDS             110..691
                     /locus_tag="PXB27_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252416.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            692..862
                     /locus_tag="PXB27_002500"
     CDS             692..862
                     /locus_tag="PXB27_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HDL5347233.1"
                     /translation="MLKCKQITELVSLSQEQKLTLKQRVNVQVHLMLCSRCRAFSQNC
                     KTVDKLMKEFNS"
     gene            complement(911..1864)
                     /locus_tag="PXB27_002501"
     CDS             complement(911..1864)
                     /locus_tag="PXB27_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family transporter"
                     /protein_id="HDL5347234.1"
                     /translation="MFGFDNLPVVYVLFTVIQVGLSINGFKGIKWLENVSCVFLIGIL
                     AYMLYVVNTKFSVEIGERFANIEGTWGMPFWAATTAFLGIYSTMILNASDYSRHVKKG
                     TTPTATGVIYVLSILPVTLFMGLIGLLVTAATGNSDPIEVFATAMDNKFLTVITLLFI
                     AFAQVTTNVLNNIVPPSYILMDSFKMKWSHATILVGILSICVMPWKLVTAESADGLSL
                     FIKIYSAFLGPIFAVMVVDYYILRKRTLNVNDLYNKEGIFKGINWAAIIAIAIGSFCS
                     LIVVDLSWYVSLIPSGLSYYILMRVLKSSEAFRKGTVLEHQ"
BASE COUNT          650 a          348 c          341 g          525 t
ORIGIN      
        1 tgttaggttt aaaaatactt cataccaata tcccctaagt tgggtaaaac cttatgaaat
       61 aggatacaag cggttagttt ctctcaattt tttaccaaag gatcacgcaa tgagccaacc
      121 ggctttacgg caaatttccc cctcgcagct agaagatatt cgtctgcaaa tggtgaagtt
      181 tgccttttta cagttaaaaa ataacgaact tgccgaagat gtggtgcaag aggcgtttgt
      241 cagcgcttat aaatatgccg acagcttcaa aggtgaaagt gcgttaaaaa cgtagatttt
      301 tgcaatttta aaaaacaaga tcgtagattt agtgaaagcc aaaggcaaat tggtggcagt
      361 atctgagctg atggaagaag acgatcacga tcttgccgat aaattgttta acgaaaatgg
      421 cggttgggat aaagatgctt atcaatctaa tgagtggaag aacaccgaaa gccataccta
      481 ttccgctcaa ttctggcaag tatttgaatt ttgcctaaat aatctgccgg caaatcaagc
      541 aagggtgttt atgatgcgtt cccatttgga aatggaaacc gaagaaattt gcgcagaatg
      601 tgagatttca ccagcaaatg tgcatacctt actgtatcgg gcaagattgc agttacaggt
      661 ctgtttaagt caaaaatggt ttggaggtta aatgttaaaa tgtaagcaaa ttaccgaatt
      721 agtttctctg tcccaagaac aaaaactgac attaaaacaa cgtgttaatg tgcaagttca
      781 tttaatgctt tgttctcgtt gcagagcctt tagccaaaac tgcaaaaccg tagataagct
      841 aatgaaagaa tttaactctt agcgtaaaag caagcggtaa aatttttaat aaattttacc
      901 gcttgtcatc ttattgatgt tctagcactg tccctttacg gaaagcttcg gaacttttta
      961 acaccctcat caaaatatag tagctcaagc ctgatggaat taaactgaca taccaagaga
     1021 gatccaccac aatcagtgag caaaacgaac cgatagcaat cgcaataatt gctgcccagt
     1081 taatcccttt gaaaatacct tctttattat ataagtcatt tacgtttaaa gtacgttttc
     1141 ttaagatgta gtaatcgacg accattaccg caaaaatagg gcctaagaat gccgagtaaa
     1201 ttttaataaa caaagataat ccatctgctg attcagcagt taccaatttc caaggcatta
     1261 cacatataga aagaatgccc actaaaatgg tggcatgcga ccatttcatt ttaaatgaat
     1321 ccattaaaat gtaagatggc ggaacaatat tattcaacac gtttgtcgta acctgtgcaa
     1381 aagcaatgaa taataacgtt attactgtta aaaatttatt atccattgca gttgcaaata
     1441 cttcaattgg gtcactattg cctgtagctg ccgtaactaa caaccctatt aaacccataa
     1501 ataaggttac agggaggatt gataatacat aaatcacacc tgttgctgtt ggggttgttc
     1561 cttttttaac gtggcgagaa taatcggaag catttaaaat cattgttgaa taaataccca
     1621 agaatgctgt agttgcagcc cagaatggca ttccccaagt gccttcaata ttggcaaagc
     1681 gctcgcctat ttctacagag aattttgtat tcacaacata aagcatataa gcaagaatac
     1741 cgatgaggaa cacacaagaa acattttcca accatttaat acctttaaaa ccattaatgg
     1801 ataagccaac ttgaataaca gtgaatagca cataaacaac aggcaagtta tcaaaaccga
     1861 acaa
//
LOCUS       DANLUQ010000232         1819 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.234, whole genome shotgun sequence.
ACCESSION   DANLUQ010000232 DANLUQ010000000
VERSION     DANLUQ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.234"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(72..1733)
                     /locus_tag="PXB27_002502"
     CDS             complement(72..1733)
                     /locus_tag="PXB27_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HDL5347235.1"
                     /translation="MSEIALTVSLLSLVAVLGLWIGHIKIRGVGLGIGGVLFGGLFVS
                     HFMAQFDVKLDAHTLHFIQEFGLILFVYSIGIQVGPGFFASLKHSGLKLNGFAVLIVL
                     ISGILVILIHKFFNVPLPVILGIFSGAVTNTPSLGAGQQVLAELSAESVTEIMGMSYA
                     IAYPFGIIGILLSMWLIRILFRINIEAEAEAFNKQQNRSKDQLSTLNVRLTNPNIHGL
                     TLKQIPDFNLHEVAYSRLKRGNELSIPRVDTILQIGDILHLVGEESSLRKMQIILGEA
                     VDVSVSTKGTIYRSERAVVTNEKVFGKQIRQLMLKGKYDVVISRLNRAGVELVPTGDM
                     TLQFGDVLNLVGRQADIEMVMTIIGNAHSKLQQVQMLPIFIGIGLGVLLGSVPIYLPG
                     FPVALKLGLAGGPLVVALILARIGSFSKLYWFMPPSANLALREIGIVLFLAVVGLKSG
                     GRFLETLLSSEGVEWICYGAIITFVPLMIAGLIARMYNKVNYLTICGLLAGSMTDPPA
                     LAFANEIKEGNGAAALSYATVYPLVMFLRIMLPQVLAIMLWVSGG"
BASE COUNT          612 a          418 c          304 g          485 t
ORIGIN      
        1 tcaataaaag ttagataaga aaaaaggcga ctttccgtcg ccttttgggc ttatatgctt
       61 gatgtctatc tttatccacc tgaaacccac aacataatcg ctagcacttg aggtagcata
      121 atacgcaaaa acattactag tgggtaaaca gttgcatagg ataacgctgc agcgccattt
      181 ccctctttaa tttcattggc aaaagcaagc gctggaggat cagtcattga gccggcaagt
      241 aatccacaaa tcgttaaata gtttactttg ttatacattc tggcaatcaa tcctgctatc
      301 atcaatggaa caaaagtaat aatcgcccca tagcatatcc actcaacgcc ctcactgctt
      361 aatagcgttt cgagaaatct accgccagat tttaaaccaa ctaccgctaa aaacagtaca
      421 ataccgattt cacgcaatgc aagattggca cttggtggca taaaccagta gagtttactg
      481 aaactaccaa ttcttgcaag aataagagca accaccagtg gtccaccagc caagcccagc
      541 tttaacgcaa cagggaaacc cggtaaataa atcggtacag aaccaagtaa tactcccaaa
      601 ccgatcccga taaatattgg caacatttgg acttgttgca actttgagtg cgcattgccg
      661 ataatcgtca ttaccatttc aatatcagct tgccgcccta ctaaattgag aacatcacca
      721 aattgtaacg tcatatcacc tgttggtact aattctacac cggctcgatt taaacgagaa
      781 atgacaacat cgtatttgcc tttaagcatt agctgacgaa tttgtttccc gaagactttt
      841 tcattcgtca ctacagcacg ttcagaacgg taaatcgtgc ctttggttga gacagataca
      901 tctactgcct cacctaaaat aatctgcatt tttcgtaatg aggattcttc acctactaag
      961 tgtaaaatat ctcctatctg tagaatcgta tctacacgtg gaatagaaag ctcattcccc
     1021 cgttttaagc gtgaataagc tacttcgtgc aaattaaaat caggaatttg tttcaacgtt
     1081 aaaccgtgaa tatttggatt agtcagacgg acatttaagg tactaagttg atctttactt
     1141 ctattttgct gtttgttaaa agcctctgct tccgcctcaa tattgattcg aaataaaatc
     1201 cgaatcagcc acattgaaag tagaattccg ataatgccga acgggtaagc aatcgcataa
     1261 ctcattccca taatttcagt tacactctca gcgcttaact cagccaatac ctgttgacct
     1321 gctcctaaag aaggggtatt ggttacagcc ccggagaaaa tgcctaaaat cacaggcaaa
     1381 ggaacattga agaatttatg aataagaata actaaaatac cgctaatcag tacaatcagt
     1441 accgcaaaac cattgagttt taagccagaa tgttttagtg aggcaaagaa tccaggtcct
     1501 acttgaattc caattgaata aacaaataaa atcagcccga actcttgaat aaaatgaagc
     1561 gtatgggcat cgagcttcac atcgaattgc gccataaaat gggagacaaa cagcccacca
     1621 aataacactc cccctatacc tagccccacg cctctgattt taatatggcc aatccataag
     1681 cctagaacgg ccactaaaga taataaactg acagtaagtg caatttcgct cattacagcc
     1741 tctctttaat atgaaatacc tcaaaaatgg tatccaattc taccaaatct caaaaacgaa
     1801 aaatgagatc tatgtcaaa
//
LOCUS       DANLUQ010000233         1801 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.235, whole genome shotgun sequence.
ACCESSION   DANLUQ010000233 DANLUQ010000000
VERSION     DANLUQ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.235"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            132..1454
                     /gene="pepP"
                     /locus_tag="PXB27_002503"
     CDS             132..1454
                     /gene="pepP"
                     /locus_tag="PXB27_002503"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HDL5347236.1"
                     /translation="MDLAYLAKMPQEEFTARRERVLEQMQDNSALLVFTESEKRRNSD
                     SEYLFRPDSYFWYLTGLAEPKSAVLLVKSEGKTESIIFVRKKDPLMETWNGRRLGIEE
                     APKTLQLNAAFDIDDIETVLAKKLENLTACYCALGIQEWGDNILFATFEKMKVNRQKV
                     PTTLIDWQPMLSEMRLIKSEFEIALIQQACHISSMAHIRAMKQARPNRYEWEIEGEIQ
                     HEFSRFGARFPAYNSIVAGGENACILHYNENDQVLKGGDLLLIDAGAEFAYYAGDITR
                     TFPINGKFSEPQKELYELTLTMQKEAINLLVPNSSIKAANDKAVQILTQGLVRLGILN
                     GDVESLIEQKAYRQFYMHGLGHWLGLDVHDVGDYGTERDRPLQIGMVLTVEPGIYIPK
                     DADVPEQYKGIGIRIEDNLLITEYGNKNLSSGCPKEIADVEQLMQSLN"
     gene            1570..1755
                     /locus_tag="PXB27_002504"
     CDS             1570..1755
                     /locus_tag="PXB27_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1508 domain-containing protein"
                     /protein_id="HDL5347237.1"
                     /translation="MYFEIYKDAKEEFRWRLKAGNHEVIATNQAYTTKQGCQKGIEAV
                     KKVTPETEVKDLTKAEA"
BASE COUNT          573 a          299 c          414 g          515 t
ORIGIN      
        1 ttaatctgtt ttgtttttgc aatcaagatc tcaaatataa tctttcatct gttttggtta
       61 ggctgaattt atgctgcgtt tgtgctaaaa taaggcaggt ttcgatatag atataataaa
      121 aaggagaaat aatggatctc gcttatttgg ctaaaatgcc acaagaagaa tttaccgcac
      181 gccgtgagcg tgtgcttgag caaatgcagg ataattctgc gttattggtt tttaccgaat
      241 cggaaaaacg ccgtaatagt gactctgaat acttgtttag acctgatagt tacttttggt
      301 atttaaccgg tttggcagaa cctaaatcag ccgtattgct ggtaaaaagc gaaggtaaaa
      361 cggaaagtat tatttttgtg cgtaaaaaag accctttaat ggaaacctgg aatggcagac
      421 gtttagggat tgaggaagct ccaaaaacgt tacagcttaa cgcagctttt gatattgatg
      481 acattgaaac cgttcttgca aaaaaattag aaaatctgac cgcttgttat tgtgcgttag
      541 ggatacaaga gtggggcgat aatattttgt ttgcaacatt tgaaaaaatg aaagtcaatc
      601 ggcaaaaagt gccgacaacc ttgattgatt ggcagccaat gttatcggaa atgcggttga
      661 ttaaatcaga atttgaaatt gccttaattc agcaggcgtg tcatatttct tcaatggcac
      721 atattcgagc gatgaaacaa gctcgcccga atcgttatga gtgggaaatt gagggggaaa
      781 ttcaacacga atttagccgt tttggagcaa ggtttccggc atataattca atcgttgccg
      841 gcggtgagaa tgcctgtatt cttcactaca atgaaaatga tcaggtgctt aaaggcggcg
      901 atttattact gattgatgca ggggcggaat ttgcgtatta cgccggcgat attacccgaa
      961 ccttccctat taatgggaaa ttctccgaac cgcaaaaaga gctgtacgaa ttaaccttaa
     1021 ctatgcaaaa agaggcgatt aatcttcttg tgccaaacag ctcgattaaa gcggcaaacg
     1081 ataaggctgt acagattctc acgcagggct tggttcgctt aggaatttta aacggtgatg
     1141 tggaaagcct gattgagcaa aaagcctatc gccaatttta tatgcacgga ttagggcatt
     1201 ggctagggct agatgttcac gatgtggggg attacggcac ggagcgggat cgtcctttgc
     1261 agattggtat ggtgttaact gttgaacccg gtatctatat tcctaaagat gcggatgtac
     1321 cggagcaata taaaggtatt gggattcgga ttgaagataa tttgctgatt accgaatacg
     1381 gcaacaaaaa cttaagcagc ggttgcccta aagaaattgc ggatgttgag cagttgatgc
     1441 aatcgttaaa ttaattcgca aatttttgta aaaaaatgac cgcttgcttt gatattttgg
     1501 ggaatttagc ttaagctaac ccgacacaaa cacagtgttt gggcaatcac aacacgaagg
     1561 agtgtttata tgtattttga aatttacaaa gatgcaaaag aagaatttcg ttggcgttta
     1621 aaagcgggta accacgaagt tattgcaacc aaccaagctt atacgactaa gcaaggttgt
     1681 caaaaaggca ttgaagcagt taaaaaagtt acacctgaaa cagaagtaaa agatttaacc
     1741 aaagctgaag cataatctat aaagccctga tattatttca gggctttgtt atttatgcaa
     1801 a
//
LOCUS       DANLUQ010000234         1796 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.236, whole genome shotgun sequence.
ACCESSION   DANLUQ010000234 DANLUQ010000000
VERSION     DANLUQ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.236"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            21..440
                     /locus_tag="PXB27_002505"
     CDS             21..440
                     /locus_tag="PXB27_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005704641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS sensor domain-containing protein"
                     /protein_id="HDL5347238.1"
                     /translation="MVPSNEAVLKAVHDTLVISTTDLQGVITYANDLFCKLTGFTREE
                     LIGQPHNIVRHPAVPKEVYKDMWDTIQAGKIWTGIVPNCGKGGVVYVVDTTVQPMFDE
                     TGKITGYISVRRVINDLMTNFEAVESAKEIFDDFYGE"
     gene            511..>1796
                     /locus_tag="PXB27_002506"
     CDS             511..>1796
                     /locus_tag="PXB27_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HDL5347239.1"
                     /translation="MSLIPHIHIAYGSESGNAEKLAQQLAIQPFLLPYSPTLSTLNET
                     DLSQLSAESLLLIVTSSFGDGEPPENADEFAEKLENLTACNLQYAIFGLGDITYDKFC
                     GYSKQLDSLLQQKQAKAVIARVDADLNYQEIFKQWLPLVEQALHSTNKETIQHSLSVQ
                     AYGEEMAYQAEVLEIKHLANSSPAVYHIRLSLKESGIFYQAGDLIYVKVQQPDRLLNE
                     YAEWFNDQNAVALLRHKELRLLSKNVLRDIQKICGSNELKDLTKISNKKALEQYVYGR
                     DLLDVLRDFDPNKSVTLADLEPILSNLAARAYSISSCGKTHPDYVDLCIRHVSYHLNG
                     RDYQGTASGYLSELQAGDFVSIFANANPNFRLPETLTAPVVMIGSGTGIAPHIAFLQA
                     LEQQYQGVESYLFFGERYRDKDFLYQTELENYLAKGT"
BASE COUNT          579 a          351 c          344 g          522 t
ORIGIN      
        1 gattcaacac ggctgaacaa attgttcctt caaatgaagc tgttttaaaa gcagtccacg
       61 atactttagt tatttcaaca actgatttac aaggtgttat tacttacgct aacgaccttt
      121 tctgcaaatt aaccggtttt acccgtgaag aattaattgg acaaccgcat aatatcgtac
      181 gccacccagc tgtaccaaaa gaagtttata aagatatgtg ggatacaatc caagccggta
      241 aaatctggac aggtatcgta ccaaactgcg gtaaaggcgg tgtagtttat gtggttgata
      301 ccaccgttca accaatgttt gatgaaacag gcaaaattac aggctatatc agcgttcgcc
      361 gtgtaattaa tgatttaatg accaactttg aagcagtaga atcagcaaaa gaaatctttg
      421 atgacttcta cggtgaataa ttaaaaaaca atgattgcgt gtaaaagatg atctctttta
      481 cacgcaattt tttattttcg aggagtttgt atgtctctca ttccccatat ccacattgct
      541 tatggttcgg agtctggtaa cgcagaaaag cttgcccaac aattagctat ccaacccttt
      601 ttacttccct actcacccac actttctaca ttaaacgaaa ccgatttaag ccaattatct
      661 gccgagagtt tattgcttat tgtaaccagc tcctttggtg atggcgaacc acctgaaaac
      721 gccgatgaat ttgcagaaaa attggaaaat ttgaccgctt gtaatctgca atatgcgata
      781 ttcgggctag gtgatattac ttatgataaa ttctgtggct atagcaaaca actagattca
      841 ttattacaac aaaaacaagc taaagctgtg attgcaaggg ttgatgccga tttaaactat
      901 caagaaattt ttaaacaatg gctaccccta gtggagcagg cattacattc aacaaacaaa
      961 gaaacgatcc aacattccct ttctgttcaa gcttacggcg aggaaatggc ataccaagca
     1021 gaagtgttgg aaattaagca tttggcaaac tcaagccctg cggtgtatca cattcgttta
     1081 tcattaaaag agagcggtat tttctatcaa gcaggggatt taatttatgt caaagtgcag
     1141 caaccagaca gactattaaa tgaatatgca gaatggttta acgatcaaaa tgcagttgca
     1201 ttactacgcc ataaagaact ccgactacta agtaaaaatg tactgcgtga tattcaaaaa
     1261 atttgcggca gtaatgaact caaagattta actaaaattt ccaataaaaa agcattggaa
     1321 caatatgtct atgggcggga tttattagac gtattacgcg attttgaccc aaacaaaagc
     1381 gtgacattag ccgatttaga gccaatttta tctaacttag ccgcacgagc ctattcaatt
     1441 agctcttgcg gtaaaactca ccctgattat gttgatttat gtatccgcca cgtttcgtat
     1501 catttaaacg gtagagatta tcaagggact gcaagtgggt atttatccga attacaagcg
     1561 ggcgattttg tctcgatttt tgcaaatgcc aatccgaatt tccgtttacc cgaaacatta
     1621 accgctccgg tggtgatgat tggctcaggt actggcattg ctccgcatat cgcattttta
     1681 caagcgttag agcagcaata tcagggggtt gaaagctatt tattcttcgg cgagcgttat
     1741 cgtgataaag attttctgta tcaaacggaa ctggaaaatt acttagcaaa aggcac
//
LOCUS       DANLUQ010000235         1632 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.237, whole genome shotgun sequence.
ACCESSION   DANLUQ010000235 DANLUQ010000000
VERSION     DANLUQ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.237"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..662
                     /gene="fnr"
                     /locus_tag="PXB27_002507"
     CDS             <1..662
                     /gene="fnr"
                     /locus_tag="PXB27_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014991492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HDL5347240.1"
                     /translation="TQLDNIIERKKPIQKSQVIFKLGDELRSLYAIRSGTLKAYTLSE
                     SGEEQITGFHLPGDLIGFDAITNMQHEGYAQALETSMICEIPFDILDDLAGKMPKIRH
                     QIMRLMSNEIKSDQEMILLLSKMNAEEKLAAFLYNLSQRYSARGFSAKEFRLTMTRGD
                     IGNYLGLTIETISRLLGRFQKSGMISVQGKYIIINRMEELAELAGAIKPQQNIIAQTT
                     A"
     gene            806..1597
                     /locus_tag="PXB27_002508"
     CDS             806..1597
                     /locus_tag="PXB27_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL5347241.1"
                     /translation="MGILTGKRILVTGLASNRSIAYGIANAMKQQGAELAFTYLNDKL
                     KPRVEEFAKEFGSDIVLPLDVATDESITECFTELSKHWEKFDGFVHAIAFAPGDQLDG
                     DYVNAATREGYRIAHDISAYSFVAMAQAARPFLNENAALLTLSYLGAERAIPNYNVMC
                     LAKASLEAATRVMAADLGKEGIRVNAISAGPIRTLAASGIKNFKKMLSAFEKTAALRR
                     TVTIDDVGNSAAFLCSDLASGVTGEVLHVDAGFSVMAMGELGDEE"
BASE COUNT          499 a          334 c          323 g          476 t
ORIGIN      
        1 taactcaact agataatatt attgagcgta aaaaaccgat tcaaaaatcg caagttattt
       61 ttaagttagg tgatgaactg cgttcgcttt atgctatccg ttctggaaca ttaaaagcct
      121 ataccttaag tgaaagtggt gaagagcaaa ttaccggttt ccatttacct ggcgatctaa
      181 ttggctttga tgcaattacc aatatgcagc acgaaggcta tgctcaagca ctcgaaacct
      241 caatgatttg tgagattcca ttcgatattt tagatgatct tgccggcaaa atgccaaaaa
      301 ttcgccatca aattatgcgt ttgatgagta atgaaattaa aagcgatcaa gaaatgattc
      361 tattgctctc aaaaatgaat gcggaagaaa aactagctgc atttttgtat aatctttccc
      421 aacgttactc agcccgtggt ttctctgcaa aagagttccg tctaactatg acccgtggag
      481 atatcggcaa ttatcttggt ttaaccattg aaactatcag ccgtttatta gggcgtttcc
      541 aaaaaagcgg tatgatttca gtacaaggaa aatatattat tattaaccgt atggaagaat
      601 tagccgaatt agccggggca atcaaaccgc agcaaaatat tattgctcaa actaccgctt
      661 aatttatctt accaaatccc tttttgaaac ctcgttcaaa aagggatttc tgatccgatg
      721 agccgctaac tccatttgcc cgtttttcct atatccgtta taatcgacac aattttgccc
      781 caatcaatat aaaagaggaa tttttatggg tatcttaact ggtaaacgta ttttagtgac
      841 aggtttagca agcaaccgtt cgatcgctta cggtattgca aacgcaatga agcaacaagg
      901 tgcagaactt gcattcacct acttaaacga taaattaaaa ccacgagttg aagaatttgc
      961 caaagaattt ggctcagaca ttgtccttcc gttagatgta gcaaccgatg aaagcattac
     1021 tgagtgcttt actgaattaa gcaaacattg ggaaaaattt gacggttttg ttcacgctat
     1081 cgcatttgca ccgggcgatc aattagacgg cgattatgta aatgccgcaa cccgtgaagg
     1141 ttatcgtatt gcacacgaca tcagtgccta tagctttgtt gcaatggctc aagctgctcg
     1201 ccccttctta aatgaaaatg ccgccctttt aaccttaagt tacttaggtg cagagcgtgc
     1261 aatccccaac tacaatgtaa tgtgcttagc caaagcctct ttagaagctg caacccgtgt
     1321 aatggcagcc gatttaggta aagaaggtat tcgtgtaaat gcgatttcag ccggcccgat
     1381 ccgtacatta gctgcttcag gcattaaaaa cttcaagaaa atgctttctg ctttcgagaa
     1441 aaccgctgct ttacgccgca ccgtaactat tgatgatgta ggtaactctg cagctttctt
     1501 atgctcagat ttagcctctg gtgtaacagg tgaagtgtta cacgttgatg ccggcttcag
     1561 cgtaatggca atgggtgaat taggcgacga agagtaattc ctataaaaat aacggactca
     1621 attgagtccg tt
//
LOCUS       DANLUQ010000236         1583 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.238, whole genome shotgun sequence.
ACCESSION   DANLUQ010000236 DANLUQ010000000
VERSION     DANLUQ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.238"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..617
                     /locus_tag="PXB27_002509"
     CDS             <1..617
                     /locus_tag="PXB27_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347242.1"
                     /translation="NLAAISANLGKVTAGTITGTTISGGTISGTTITGGIIKGARIEG
                     LTGKFTGELEINQLIGGNIYEAKTWTSKATGKSKTYSETKNSFALSDTYYEYVCQFVL
                     PATKATRTLYVVHTPTGESKSETFYGSGSNRTPTTITLPSVIDISNTISPNVSNPFND
                     SGAILIANTQYVVTCRAWCAGRGRSAKVNFRAIISSTNGSSYQL"
     gene            627..785
                     /locus_tag="PXB27_002510"
     CDS             627..785
                     /locus_tag="PXB27_002510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347243.1"
                     /translation="MKKIILLTTIMVSACTTQYKQDYTTKPTFCYQLAPQEQLPGKNC
                     IGSGGHSG"
     gene            complement(794..1093)
                     /locus_tag="PXB27_002511"
     CDS             complement(794..1093)
                     /locus_tag="PXB27_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL5347244.1"
                     /translation="MKTLDQMLNELSPERRQKVEDMAQEMIREIQLSKLREELNLSQQ
                     QLATALGISQPAIAKLEQKDNDPRLSTLKRYIEAMGGKLKLAVELPTGDERVIRI"
     gene            complement(1065..1454)
                     /locus_tag="PXB27_002512"
     CDS             complement(1065..1454)
                     /locus_tag="PXB27_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL5347245.1"
                     /translation="MADPIEWSIITTDCFDEWFGQQDGSTQERVLACLIYLRKFGYSL
                     GRPYADTVYQSKFPNMKELRIQNKGKPIRAFYAFDPLRQAIVLCAGEKGKDKRFYEKM
                     IALADAEFTSYLNNLEKENENVRSNAQ"
BASE COUNT          514 a          282 c          298 g          489 t
ORIGIN      
        1 acaatttagc ggcaatatcg gcaaatcttg gtaaggtaac tgctggtaca attacaggga
       61 cgacaattag tggtgggaca atttcaggta caacaattac agggggaata attaaaggag
      121 caagaattga gggattaaca ggtaaattta ctggagaact tgaaatcaac caattgatcg
      181 gaggtaatat ctatgaggct aaaacttgga catcaaaagc aacagggaaa agtaaaacat
      241 attcagaaac gaaaaacagt tttgcattaa gtgatacata ttatgagtat gtatgtcaat
      301 ttgtactacc tgctacaaaa gcgacgagaa cattgtatgt tgtccacact ccaacaggag
      361 agagtaaaag cgaaactttt tatggtagtg gtagtaatcg gacaccgaca acaattacat
      421 taccgtctgt aattgatatt agcaatacta tttcacctaa tgtatctaac ccatttaatg
      481 attctggggc aatactcatt gctaatactc aatatgtagt aacgtgtcgt gcgtggtgtg
      541 cagggcgtgg gagaagtgct aaagtgaatt ttcgagcaat tatatcatct acaaatggtt
      601 catcttatca attataggag gcaaatatga aaaaaattat actacttaca acaattatgg
      661 tttcagcttg tacaactcaa tataagcaag actatacaac aaaaccaacc ttttgttatc
      721 aactagcccc tcaagagcaa ttaccgggga aaaactgtat tggctcagga gggcattcag
      781 gttaacaaag taattaaatc ctaattacac gctcatcgcc tgtaggcaat tcaacggcaa
      841 gttttaactt accgcccatt gcttcaatat agcgtttgag cgtggataat cttggatcgt
      901 tatctttctg ttcaagttta gcaatagcag gctgtgaaat accgagagct gtagctaatt
      961 gttgttgaga gagatttagc tcttctctta gttttgaaag ttgtatctct ctaatcattt
     1021 cttgagccat atcttccact ttttggcggc gttcaggaga gagttcattg agcatttgat
     1081 ctaacgtttt cattttcttt ctctaaatta tttaaatagg aggtaaattc cgcatcagca
     1141 agagcgatca ttttttcata gaatcttttg tctttacctt tctcgccagc acaaagtact
     1201 atcgcttggc gtaaagggtc aaaagcataa aatgctcgaa ttggcttacc tttgttttga
     1261 atgcgtaact ctttcatatt agggaattta gactgataaa ctgtatcagc ataaggtctg
     1321 cctagactat aaccgaattt ccgcagatag attaaacaag ccaagacacg ttcttgagta
     1381 cttccatctt gttgaccgaa ccattcatca aagcagtcag tcgtgataat agaccattct
     1441 atagggtctg ccatttttga accttttata acttactatt tataaatgaa gtataaataa
     1501 aaggttatat tatgtaaagc aatttactct tcctactaag ccgtttgtat gactacaagc
     1561 ggtctttttt tatcaacaat cca
//
LOCUS       DANLUQ010000237         1582 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.239, whole genome shotgun sequence.
ACCESSION   DANLUQ010000237 DANLUQ010000000
VERSION     DANLUQ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.239"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            70..753
                     /locus_tag="PXB27_002513"
     CDS             70..753
                     /locus_tag="PXB27_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL5347246.1"
                     /translation="MNQTRDRLEKLPLFHKMQQFIIKALKIIAIFPLLGIAILLVVDS
                     LTSYIVRDKIYTDSKQIPYREYAVVLGTAKFYSKDVINQYYKYRLEAAKQLVQEGKVN
                     QLLLSGDNKTPYYNEPKTMTSDLLKMGIATSKIKQDYAGYTTFYSIIRAKKVYKLPPF
                     TIISQKFHCERALFIAKFRDIDAICYAATYPEGAYQVRMREILARSAMVFSLLIGKEP
                     ESLEDIRSK"
     gene            complement(790..>1582)
                     /gene="purU"
                     /locus_tag="PXB27_002514"
     CDS             complement(790..>1582)
                     /gene="purU"
                     /locus_tag="PXB27_002514"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HDL5347247.1"
                     /translation="GLVAKITNICYKHQLNIIKNSEFVENETKRFFMRTELNGIFNDE
                     TLLTDLKFTLPEGSSYKLLPKQRKRIVILVTKEAHCLGDLLMKNYYGGLDVEIAAVIG
                     NHETLKSLVERFDIPFHLVSHENLTRVEHDKLLAEKIDEYSPDYIVLAKYMRVLNPEF
                     VARYPNRVVNIHHSFLPAFIGAKPYHRAYERGVKIIGATAHFVNDELDEGPIIMQNVI
                     NVDHTYTAEAMMRAGRDVEKTVLSQALELVLADKVFVYKNKTIIL"
BASE COUNT          509 a          262 c          297 g          514 t
ORIGIN      
        1 cgattagact attttgtttg tctctaattt acccatctta aatagggtag caaaagtaaa
       61 ggatctcatt tgaaccaaac gcgtgatagg ttagaaaaat tgccactttt ccataaaatg
      121 cagcagttta ttattaaggc attgaaaata attgcaattt tccccttatt gggaatagct
      181 atattattag tggttgattc acttacaagt tatattgtga gggataaaat ttatacagat
      241 agtaaacaga ttccttaccg ggagtatgca gttgtactcg gtacggcaaa attttactct
      301 aaagatgtga ttaaccaata ttataaatat cgtttggaag ccgctaagca attagtccaa
      361 gaaggaaagg tcaatcagct tttgttaagt ggagataata aaacgccgta ctataatgag
      421 ccaaaaacaa tgacaagtga tctacttaaa atggggattg cgacttcaaa aattaagcag
      481 gattatgcgg ggtataccac tttttattca ataattaggg ctaaaaaagt atataaatta
      541 ccgccattta cgattatctc ccaaaaattc cactgtgaaa gggcattgtt tattgcaaaa
      601 tttagggata ttgatgcaat ttgttatgcc gctacatatc cagagggggc ttatcaggta
      661 cgaatgcggg aaattttagc cagatcggca atggttttta gtttgttgat aggtaaagag
      721 cctgaatcat tggaagatat ccgttctaaa tgagatagcc ggtcagataa tgttgaccgg
      781 ctattttgct tataaaataa tggttttgtt tttatataca aatactttgt cagcaagcac
      841 taattctaag gcttggctaa gtacggtttt ctcgacatca cgtccggcac gcatcatagc
      901 ctctgcggtg taggtgtgat caacattaat cacattttgc ataataatcg ggccttcatc
      961 taattcatca ttcacaaaat gggcggttgc accgataatt ttcactccac gttcgtatgc
     1021 acgatgataa ggttttgcac caatgaaagc gggtaagaac gagtggtgaa tattgacaac
     1081 tcggtttgga taacgagcga caaattccgg attcagcacc cgcatatatt tagccaacac
     1141 aatataatca ggtgaatatt catcaatttt ttccgctaat aatttatcgt gttccactcg
     1201 ggttaaattt tcgtggctga ctaaatggaa tggaatatcg aaacgttcaa ctaaactttt
     1261 taatgtttcg tggtttccaa tcacggcagc aatttctaca tctaatccac catagtaatt
     1321 tttcatcagt aagtcaccta aacaatgtgc ttctttggta actaaaataa cgatacgttt
     1381 tctttgttta gggagtaatt tatagcttga gccttcaggt aaggtaaatt ttaaatcagt
     1441 gagtaaggtt tcatcattaa aaattccgtt taattcggta cgcataaaaa aacgtttagt
     1501 ttcattctca acaaattcag aatttttaat aatatttaat tggtgcttat agcaaatatt
     1561 ggtaatttta gcgacaagtc ct
//
LOCUS       DANLUQ010000238         1570 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.240, whole genome shotgun sequence.
ACCESSION   DANLUQ010000238 DANLUQ010000000
VERSION     DANLUQ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.240"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            54..1499
                     /gene="aldA"
                     /locus_tag="PXB27_002515"
     CDS             54..1499
                     /gene="aldA"
                     /locus_tag="PXB27_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HDL5347248.1"
                     /translation="MVKQHKMYINGEFVPALDGRTLAVYNPATEKAISEVPRGGADDA
                     KIAIDAAEAAQEAWERLPAIERGKYLRQIAVGIRERADEIARTISEEMGKTLSLATVE
                     VHFTADYLDYMAEWARRYEGEIIQSDRPNEHIFLYKKAIGVTTGILPWNFPFFLIARK
                     AAPALVTGNTIVIKPSEDAPNNAILFSEIVHQVGLPKGVFNVVTGYGNEVGPELSGNE
                     KIGMVSFTGSQFAGQKVMEAASKNIIKVNLELGGKAPAIVLDDADLELAATAVVNSRI
                     INSGQVCNCAERIYVQSSIKEQFVQKLVEKMQAVKYGNPLEQQDIDMGPMVNEKGLHK
                     VQEMVTHALHQGGNLVLGGNVVEGTGYYFEPTIIDNVDNSMDIMRHEIFGPVIPVATF
                     DTIDEAIRLANDCEYGLTSSVYTQNINKAMKVISRLKFGETYVNRENFEAMQGYHAGW
                     RKSGIGGADGKHGLEEYLQTQVVYLQFDDKV"
BASE COUNT          482 a          302 c          365 g          421 t
ORIGIN      
        1 agttaccgaa tgatagtcat caacttccac tgtggataaa aaaggagcaa ctcatggtga
       61 aacaacataa aatgtatatt aatggtgaat ttgtgcctgc gttagatggt cgcactttag
      121 cagtctataa ccctgccacc gaaaaagcta tctccgaagt ccctcgtggc ggagctgatg
      181 atgccaaaat cgctatcgat gctgctgaag ccgcccaaga agcttgggaa cgtttaccag
      241 cgattgaacg aggaaaatat ttacgtcaaa ttgctgtggg gattcgtgag cgtgccgatg
      301 aaattgctcg cactatttcc gaagaaatgg gtaaaactct gtccttagca accgttgagg
      361 tacattttac cgctgactac ttagactata tggcagaatg ggcgagacgt tatgaagggg
      421 aaattatcca aagtgatcgc ccaaatgaac atattttcct atacaaaaaa gcgattggcg
      481 tcaccaccgg cattttacct tggaacttcc cgttcttcct cattgcccgt aaagcggctc
      541 cggcattagt aacaggtaat actattgtga ttaaaccgag tgaagatgca cctaataatg
      601 cgattctctt ttcggaaatc gtgcatcaag tcggtttacc taagggggta tttaatgtcg
      661 tcaccggcta tggcaatgaa gtcgggccgg aactttcagg caatgaaaaa atcggtatgg
      721 tttcctttac cggtagccag tttgccggac aaaaagtgat ggaagccgcc agcaagaata
      781 tcattaaagt caatttggaa ttgggcggaa aagcaccggc tatcgtatta gacgatgcag
      841 atcttgagct tgctgctacc gctgtagtaa attctcgaat catcaattca ggacaggttt
      901 gtaactgtgc agaacgcatt tatgtacaga gttcaattaa agagcaattt gtccaaaaat
      961 tggtagagaa aatgcaagcg gtgaaatatg gtaatccatt agaacaacaa gacattgata
     1021 tggggccgat ggtaaatgaa aaaggcttgc ataaagtgca ggaaatggtg acacacgcac
     1081 tgcatcaagg aggtaatctg gtattaggtg gtaacgtggt tgaaggaaca ggctactact
     1141 ttgagccaac tattatcgac aatgtagata actctatgga tattatgcgt cacgaaattt
     1201 ttggtccggt cattccggtt gcgacatttg ataccattga tgaagcgatt cgcttagcca
     1261 atgattgtga atatggctta acctcttcgg tttacacgca aaatatcaat aaagcgatga
     1321 aagtaatttc ccgcttaaaa ttcggagaaa cttatgtaaa ccgtgaaaac tttgaagcaa
     1381 tgcaaggcta ccacgccggt tggcgtaaat cgggtattgg cggtgctgac ggtaagcacg
     1441 gcttagagga atacctacaa actcaggtgg tttatctgca atttgatgat aaagtataga
     1501 ttcttctaaa aactcccact tatcaaaata agcgggagtt ttcttttagg ttaaccttgt
     1561 atcaaaattg
//
LOCUS       DANLUQ010000239         1564 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.241, whole genome shotgun sequence.
ACCESSION   DANLUQ010000239 DANLUQ010000000
VERSION     DANLUQ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.241"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..224
                     /locus_tag="PXB27_002516"
     CDS             <1..224
                     /locus_tag="PXB27_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347249.1"
                     /translation="QNKNLTDESEEQAILAYLTENIMARIEKEQPLEKTLILYSEQLA
                     SFEQKSPMALHREKFEAKPTGLAELINNL"
     gene            235..1185
                     /locus_tag="PXB27_002517"
     CDS             235..1185
                     /locus_tag="PXB27_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HDL5347250.1"
                     /translation="MSLSTFAHALKLDKQGNVILFDDSPEEQAKLISRLKQNRLFKLL
                     NLVQTKHAKGEAIGLFTPIAGTTNTESGEEREPKQALAPAQKYHCEQINIDSYIRYEK
                     IDAYNLENSLEERLNQLLDDNLLRGLLMVGWQGVSRADTSNATNNQLAQDVKKGWLQK
                     IREGKPEAVINGATIGKEQQYKSLNALIKQGLGKISPAYAMGGDMIAICGRDIIADDV
                     IQLEHNDLSENLADLLTLCQQAKGGLKAVSIPYFPSNAILITRLDNLSLYLHLNNIRR
                     KAFDTPQKDSVETFYSLNVDYIVEDFNACCLIENIEVIEE"
     gene            1182..1376
                     /locus_tag="PXB27_002518"
     CDS             1182..1376
                     /locus_tag="PXB27_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347251.1"
                     /translation="MSLESYIQQAEEMELLEKLDFVSDLLELEKVSEIEAYRILTAHG
                     EPKPQPAQEKLIGCVDFLDD"
BASE COUNT          584 a          325 c          296 g          359 t
ORIGIN      
        1 aacaaaataa aaacctcaca gatgaaagtg aagagcaagc tattctggca tatctaacag
       61 aaaatataat ggctagaatt gaaaaggaac agccacttga aaaaacatta attctttatt
      121 ctgaacagtt agcaagtttt gaacaaaaat cgccaatggc attacaccgt gaaaaatttg
      181 aagcaaaacc taccggctta gctgaattaa ttaataactt ataagaggta aaaaatgagt
      241 ttatccactt tcgcccacgc attaaaacta gataaacaag gtaacgttat tttatttgac
      301 gatagccccg aagaacaggc aaaactaatt agccgcttaa aacaaaatcg cctttttaaa
      361 ttattgaatt tagtgcaaac caagcacgca aagggcgaag cgattgggct attcacaccg
      421 atagccggca caaccaacac cgaaagcgga gaggaacgag agccaaaaca agcccttgca
      481 cccgctcaaa aataccactg cgaacaaatc aacattgata gctatattcg ctatgaaaaa
      541 atcgatgctt acaacctcga aaacagctta gaagaacgct taaatcagtt acttgatgac
      601 aaccttctaa gagggctttt aatggttggc tggcaaggcg taagccgtgc agatacatca
      661 aacgcgacaa acaaccaact cgcccaagac gtgaaaaaag gctggctaca aaaaatcaga
      721 gagggcaagc ccgaagcggt aattaatgga gcaaccatag gcaaagagca acaatacaaa
      781 agcctaaatg ctctgattaa gcagggatta ggcaaaatta gcccagcgta tgcaatgggc
      841 ggagatatga tcgccatttg tgggcgggat attatcgctg atgatgtaat tcagcttgaa
      901 cacaacgatt taagcgaaaa cctagccgac ttattaacct tatgccaaca agctaaaggc
      961 ggactaaaag cggtaagtat tccctacttc ccaagcaatg cgattttaat cactcgatta
     1021 gataaccttt cgctatatct tcatttaaac aatatccgcc gtaaagcgtt tgatacgcca
     1081 caaaaagaca gcgtagagac attttactcc ctcaatgtgg attacattgt agaagatttt
     1141 aacgcctgtt gtttaattga aaatattgag gttattgaag aatgagccta gaaagctata
     1201 tccaacaagc ggaagaaatg gagcttttag aaaaactcga ttttgtttca gacctgttag
     1261 aacttgagaa agtcagcgaa attgaagcct atcgcatttt aaccgcccac ggcgaaccta
     1321 aaccccaacc ggcacaagaa aagctaatag gttgcgtaga ttttcttgat gactaaccca
     1381 agccccgaaa ggggcttttt gcatttttgc aacaaaaaac gaccgcttgc accctttgaa
     1441 attattgcta tcattaataa aaaacaataa ccaaatcaaa tgagagtaca gcaatgacaa
     1501 actataaact accggctaga aagtggtaca ccttagagca ggcaacaaaa cgcattaaac
     1561 aact
//
LOCUS       DANLUQ010000240         1537 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.242, whole genome shotgun sequence.
ACCESSION   DANLUQ010000240 DANLUQ010000000
VERSION     DANLUQ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.242"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..429)
                     /locus_tag="PXB27_002519"
     CDS             complement(<1..429)
                     /locus_tag="PXB27_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347252.1"
                     /translation="MLPRKIKNKWLEPEKQKWIEELLRLWGAWEFGGLDLESRVNMIY
                     RLMKSAEGVKSLSSREVCNDSLGTLINEIFTVVTKRDVMLADILKQKYWFGRSERQIA
                     IYYQLRDSEGRKERRWQEIIQDKCKKAERIIADILESKISS"
     gene            complement(419..988)
                     /locus_tag="PXB27_002520"
     CDS             complement(419..988)
                     /locus_tag="PXB27_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HDL5347253.1"
                     /translation="MANKPPKQHKCKECGSYYIKFQSTQQVCSVKCAMAMGKRKTEAK
                     RKKSAKIDRLEQRKRLEKLKSRRDFEKDLQKIFNKFIRLRDKDLPCISCGRYHQGKYD
                     AGHYKTVGGNPELRFNEDNCHSQCVPCNRHLHGNIVNYRVNLIEKIGLERVEFLERKD
                     HPPLKLTIEQIKDLIKVYKAKCKELERVT"
     gene            complement(1116..1310)
                     /locus_tag="PXB27_002521"
     CDS             complement(1116..1310)
                     /locus_tag="PXB27_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347254.1"
                     /translation="MTKFFETIAFVMLFGIIPSCLLAINTTLMNMNIKNAPWFMVIIQ
                     LGFNITCYWLWFISVMRNVI"
BASE COUNT          464 a          362 c          229 g          482 t
ORIGIN      
        1 ggatgaaatc ttgctttcaa gaatatctgc aataattctt tccgcttttt tgcatttatc
       61 ttgaataatt tcttgccatc gtctttcttt tctaccctcg ctgtccctta gttgataata
      121 aatagcgatt tgccgctccg aacgcccgaa ccagtatttt tgtttgagta tatcggctaa
      181 cattacgtct cttttggtga ctaccgtaaa aatctcattg atcagcgtgc cgaggctatc
      241 gttacaaacc tctctagatg aaagactttt aaccccctcc gcagatttca tcagccggta
      301 aatcatattt acccgacttt ctaaatccaa tccgccaaat tcccacgccc cccataacct
      361 tagcaactct tcgatccatt tctgcttttc aggctctaac cacttgtttt taatttttct
      421 aggtaacacg ctcaagctcc ttgcacttcg ctttatagac tttgattaaa tctttgattt
      481 gttcgatagt aagttttaat ggtgggtggt ctttacgctc taaaaattcc acacgctcaa
      541 gcccgatttt ctcaatcaga tttactcgat aattcacaat attgccgtgc agatgtcggt
      601 tacacggcac acattgactg tggcaattat cttcattaaa acgtagctca ggattaccac
      661 caacggtttt ataatgccct gcatcatatt tcccctgatg atagcggcca caggaaatac
      721 agggtaaatc tttatcacgc aagcgaatga atttattaaa tatcttttgt aaatctttct
      781 caaaatctct acggcttttc aatttttcca gccgcttccg ttgctccaag cggtcgattt
      841 ttgcactttt tttgcgtttc gcctctgtct tacgcttacc cattgccata gcacacttaa
      901 cagagcagac ctgctgcgtg ctttgaaatt tgatgtaata actgccgcac tctttgcatt
      961 tgtgttgttt aggtggtttg ttagccattc caaccctcac catctctctt atcaaaccac
     1021 tcaataaaaa agagtgaacc ggcacataca ctaagaatag cgatggcgat taaaattaca
     1081 gtcagttcat tgcactggct cacttattaa tctccttaaa taacattccg cataacagaa
     1141 ataaaccaca accaataaca agtgatatta aaacctaact gaataatcac cataaaccac
     1201 ggtgcgtttt taatattcat attcattaaa gtagtattaa tcgccaacaa acagcttgga
     1261 ataatgccaa ataacatcac gaatgcgata gtttcaaaaa attttgtcat cttacttctc
     1321 cttcccccga attcgaggga attaataaac acacactaaa tcggagcaaa accgcgctta
     1381 aaataataaa gccattcttg atatgtccat tgacagaaaa caataaaact accgactgct
     1441 gcaaagaatg ctaatggata aatcaataaa taacctacat atctaacagt aagaatgact
     1501 aagaaaaaaa gcactgcaat aattctgaat ggcacgc
//
LOCUS       DANLUQ010000241         1535 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.243, whole genome shotgun sequence.
ACCESSION   DANLUQ010000241 DANLUQ010000000
VERSION     DANLUQ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.243"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..400
                     /locus_tag="PXB27_002522"
     CDS             <1..400
                     /locus_tag="PXB27_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347255.1"
                     /translation="VTDSRQSIFSSFWVKVWIILLPLALFYCGYNLYIFLNGSESEVS
                     HNTEQAKFASDENHNDIVIHLLPTIGKPLSTKWRITGELKRDNKSFVILADNENRLRL
                     EPSSQFSFSGRMLQGEIDGGIVSYFSGESK"
     gene            397..1530
                     /locus_tag="PXB27_002523"
     CDS             397..1530
                     /locus_tag="PXB27_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspD"
                     /protein_id="HDL5347256.1"
                     /translation="MKIHQLGLLLMISSFSYAKNVDFKLEAVPLPKAISIIYDEVLQK
                     PYMLDPKLSNDSRVISFHTTEQQNFEQFIQRYFSNMNISVSEKKGVVYIGHIEPKQPK
                     VIKRSFVYSPIHRDVDYLASFLKDGTEGNQVFASGDKLVYYGTSEDIARVKSILKSID
                     TKSREVVVTGYVFEVQNIEKEGSGINLLAKLLSGKLGISVGYKQSYENFITVNAGNLD
                     AMFELFRTDQRFRVVSSPTLRVKSGSRGNFSVGSDVPVLSNVTYQDGRPIQSIEYRSS
                     GVIFDIQPTIKDKAIDLKIQQLSNFIKTDTGVNNSPTLVKRDLVTDVTVKSGDVIVLG
                     GLAENKISNGETSFSLLPKGWLSGKSNENTKTDIIVLLQVKEI"
BASE COUNT          524 a          216 c          316 g          479 t
ORIGIN      
        1 ggttacagat agtaggcaat ctatattttc atctttttgg gtcaaggtat ggattattct
       61 tttaccattg gcattatttt attgtggcta taacctttac attttcctaa atggttcaga
      121 gagtgaagta agccataata ctgaacaggc taaatttgct tctgatgaaa accataacga
      181 tatagtaatt catttacttc ctactattgg aaaaccttta tcaactaaat ggcgaataac
      241 aggggaacta aaaagggata ataaatcatt tgtgatctta gctgacaatg aaaaccgttt
      301 aagacttgag cctagtagcc agtttagttt tagcggtcga atgttgcaag gagagataga
      361 tggtggaata gttagttatt tttcgggaga atctaaatga aaatccatca attagggcta
      421 ttgctcatga ttagctcatt ttcttatgct aagaatgttg attttaaatt agaagctgtg
      481 ccgttaccga aagcaatatc aataatatac gatgaagtgc tacaaaagcc atatatgctt
      541 gatcctaaat tatctaatga ttcaagggtt attagctttc atacaacaga gcaacagaat
      601 tttgagcagt ttattcaacg gtatttcagc aatatgaata tcagtgtatc agagaaaaaa
      661 ggtgttgttt atattggaca tattgaacca aaacagccga aagtaataaa acgtagtttt
      721 gtgtattcgc ctattcatcg tgatgttgat tatttggcta gcttcctgaa agatggaaca
      781 gaaggaaacc aagtttttgc aagtggcgac aagcttgtat attatggtac ttctgaagat
      841 attgcacgag tgaaaagtat cttgaaatca atcgacacaa aaagccgtga agtagtggta
      901 actggttatg tttttgaagt gcagaatatt gaaaaagagg gtagtggcat aaacttgtta
      961 gcgaaattgc tatcaggaaa attaggcatt agtgttggtt acaagcaaag ttatgaaaac
     1021 tttatcaccg tgaatgcagg aaatttagat gcaatgtttg aattgtttcg cacagatcag
     1081 cgttttaggg ttgtcagcag tccgacatta agagtaaaat caggctcaag aggtaatttt
     1141 tcagtaggct ctgatgtccc tgtattatcg aatgtaacct atcaggacgg aaggcctatt
     1201 cagtcgattg aataccgttc aagcggtgta atatttgata ttcagccaac gataaaagat
     1261 aaagcgattg atttaaaaat tcaacaacta tcaaatttca tcaaaacaga taccggtgta
     1321 aataatagcc caacgttggt aaagcgtgat ctggtaacag atgttacggt taaaagcggt
     1381 gatgttatcg ttttaggtgg gttagctgaa aataaaatat ctaatggcga aaccagtttt
     1441 tcattgctac ctaaaggttg gttaagtgga aaatcaaatg aaaatactaa aacagatatt
     1501 atagttctgc tacaagtaaa ggaaatttaa tatgt
//
LOCUS       DANLUQ010000242         1492 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.244, whole genome shotgun sequence.
ACCESSION   DANLUQ010000242 DANLUQ010000000
VERSION     DANLUQ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.244"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(148..1383)
                     /locus_tag="PXB27_002524"
     CDS             complement(148..1383)
                     /locus_tag="PXB27_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HDL5347257.1"
                     /translation="MKINRIILQSCSECGQQVRVPLTSNGNAVCPVCHNILRKSNTWS
                     LHRSAFLALAILILLPFALTFPLMSIDLLGIKINATVWDGIWKMATEGYPYTAFMVLV
                     CAVIMPIAFVLLILTIQLQRIIRQRPRYTLIFLTKIKEWVMLDVYLVALAVAAFKIRD
                     YADLQFDINLIAFVLVTLLNTLLFIKTEPKQAWERFYPEYHALPFDHPSNPTLCPTCE
                     YCFDETILDLKGRQRCPRCESNLSISDNVKIQRVWACLIAGTIMMIPANIFPISTTEL
                     AGQVSADSLMSGVLLFMEMGSYTVAAIVFIASIAVPVSKITIIFYLLLAIHYKWKHPI
                     HWQMKLLHYVHFVGRWSMLDLFVLSLMMSLLERGQILSFSVGEAAFYFGAAVFLTMFA
                     SANLDSRMLWKIHTVQKTS"
BASE COUNT          453 a          286 c          309 g          444 t
ORIGIN      
        1 taaagactat tttatatttg ctaatttctt tctggctatc ggtgctacat tatcggctct
       61 ttcttactca atagggattg agattttggg ttacgtacta cttttcttac tttgcatgtt
      121 atgtattatt tatcggaaaa tctttattta ggatgttttc tggacagtat gaatcttcca
      181 caacattctg gaatctaaat ttgcagaggc aaacatcgtt aagaatacgg ctgctccaaa
      241 atagaaagcg gcttccccta ccgagaaact gagaatctgc cctcgttcga gtagcgacat
      301 catcaatgat aacacgaata aatccagcat tgaccagcgt ccgacaaaat ggacgtagtg
      361 tagtagtttc atttgccaat gaatcgggtg tttccacttg taatgaattg ccagcaagag
      421 ataaaaaata atggtaattt tgctcaccgg caccgcaata ctagcgataa acacaatcgc
      481 tgccacggtg tagctaccca tttccataaa cagaagcacg ccggacatta atgaatctgc
      541 cgaaacctga cctgccagtt cagttgtcga aatcgggaaa atatttgccg gaatcatcat
      601 aatagtccct gcaattaagc aagcccaaac ccgttgtatt tttacgttgt cggaaataga
      661 taaattgctt tcacaacgag ggcagcgttg ccgtcctttt aagtctaaaa tagtttcatc
      721 aaagcaatat tcacaggtcg ggcaaagtgt tggattgctt gggtgatcaa acggcaaggc
      781 gtgatattct gggtaaaaac gttcccatgc ttgttttggc tcggttttaa taaaaagtag
      841 ggtgtttaag agtgttacca acacaaaggc gattaagttg atatcgaatt gtagatcggc
      901 ataatctcga attttaaaag cggcaaccgc aagagcaact aaatagacat caagcattac
      961 ccattctttt attttagtta agaaaatcag cgtgtaacgg gggcgttggc gaataatacg
     1021 ttgtagctga atggttaaaa ttagcaatac aaatgcaatc ggcattatta ccgcgcagac
     1081 tagcaccata aaagcggtgt aagggtagcc ctccgttgcc atcttccaaa taccgtccca
     1141 tacggtagca ttaattttga tgccgagtaa gtcaatgctc atcaagggaa aagtaagtgc
     1201 gaagggaagc aaaattaaaa tggctaatgc caaaaaagca ctacggtgca agctccaagt
     1261 attgcttttg cgtaatatat tatggcagac cgggcaaacg gcattgccat ttgacgtgag
     1321 cgggactcgc acttgttgcc cacattcgct acagctttgt aaaatgattc tgtttatttt
     1381 cattttttga tcttctatta aattaggtgg ttattttcta ctttatcgta gagattgcaa
     1441 gtggttaaat ttggcaaaat ttttgcaagt tgcattttat gcaatatgaa at
//
LOCUS       DANLUQ010000243         1468 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.245, whole genome shotgun sequence.
ACCESSION   DANLUQ010000243 DANLUQ010000000
VERSION     DANLUQ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1468
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.245"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..>1468
                     /locus_tag="PXB27_002525"
     CDS             <1..>1468
                     /locus_tag="PXB27_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HDL5347258.1"
                     /translation="DKKWPEIKKTLTKRYNFAIKRLTQVKADDILQTYLNSFSREIDP
                     HTSYLSPRAAKAFQESMNLSLEGIGATLTQEDDVTSIRALVPGAPAAKSKKLVVGDKI
                     VGVGQGERGEIEDVIGWRLDNVVDKIKGKKGTKVRLEIEPEKGGKTKIITLVRDKVRI
                     EDSAAKLTVEKVDGKNVGVIKIPTFYIGLTSDVRKLLTQIEKKNVEGLIIDLRENGGG
                     SLTEVIELTGLFIKDGPVVQVQDAFNRIKVHEDPESKSVYDGKVMVMINRHSASASEI
                     FAAAMQDYNRAIIVGQQTFGKGTVQQSRSLNFVYDLDQEPLGFIQYTIQKFYRINGGS
                     TQLKGVEADIKFPEIINAAKTGESFEDNALPWDKIPAANYKEAGNARNAVAALTAKHQ
                     ERIAKNPEFIVLNENIAIRKERDERKFLSLNLEERLKENKADESKRLKDINARFAREG
                     KKALKDIDDLPKDYEAPDFFLKEAEQMMLDWSEFDKKS"
BASE COUNT          515 a          224 c          327 g          402 t
ORIGIN      
        1 aagataaaaa atggcctgaa attaagaaaa cactcactaa acgttataac tttgcgatta
       61 agcgtttaac tcaagttaag gctgatgata ttttacaaac ttacttaaat tcattctctc
      121 gtgagatcga tccccacact agctatttgt ctcctcgtgc ggcaaaagcg ttccaagaga
      181 gcatgaatct gtcgctagaa ggaattggtg caacgctaac ccaagaagat gatgtaacca
      241 gcattagagc attagtgcca ggagcccctg ccgctaaaag taagaagctg gtagtcggcg
      301 ataaaattgt cggtgtaggt caaggtgaga gaggtgagat tgaagatgtt atcggttggc
      361 gtttggataa tgtagtcgat aaaatcaaag gtaaaaaagg cactaaagtt cgtttagaaa
      421 ttgagcctga aaaaggtgga aaaactaaaa ttattaccct tgtgcgtgat aaagttcgta
      481 ttgaggatag tgctgctaaa ttaacggtag aaaaagtaga tggaaaaaat gtaggggtaa
      541 ttaagatccc tactttttat atcggtttga cttcagatgt acgtaaatta cttactcaaa
      601 tagagaaaaa aaatgttgaa ggattgatca ttgatttacg tgaaaacggt ggtggttcgt
      661 taactgaagt gattgaatta actggtttat ttattaaaga tgggcctgta gtgcaagtac
      721 aagacgcatt caatcgtatc aaagtacacg aagatccaga atcaaaatca gtttatgatg
      781 gaaaagtgat ggtgatgatt aatcgccata gtgcttcagc ctctgaaatc ttcgctgcgg
      841 caatgcaaga ttacaaccgt gcaattattg tagggcaaca aacctttggt aagggaactg
      901 ttcaacagag ccgttcattg aattttgtat atgatttgga tcaagagcct cttggtttta
      961 tccaatatac gattcaaaaa ttctaccgta tcaatggtgg aagtacccag ctaaaagggg
     1021 ttgaggcgga tattaaattc ccggaaatca ttaatgctgc aaaaacaggt gaaagttttg
     1081 aagataatgc gcttccttgg gataaaattc cagcagctaa ttataaagag gctgggaatg
     1141 ctcgtaacgc ggttgctgca ttaacagcga aacatcaaga gcgtattgct aaaaacccag
     1201 agtttatagt attaaacgag aatattgcga ttcgaaaaga acgtgatgag cgtaagtttt
     1261 tatcgttaaa tttagaagaa cgcctgaaag aaaataaagc agatgaaagc aagcgcttaa
     1321 aagatattaa tgctcgtttt gcccgtgaag gcaaaaaagc attaaaagat attgatgatt
     1381 taccaaaaga ctatgaagca ccggactttt tcttaaaaga agctgaacag atgatgcttg
     1441 attggtcaga atttgataaa aaatcata
//
LOCUS       DANLUQ010000244         1465 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.246, whole genome shotgun sequence.
ACCESSION   DANLUQ010000244 DANLUQ010000000
VERSION     DANLUQ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.246"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            24..347
                     /locus_tag="PXB27_002526"
     CDS             24..347
                     /locus_tag="PXB27_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL5347259.1"
                     /translation="MKLSSELQNQINQLNQLRKQALKQEQDEQKLFEKNKIEDLPHFG
                     TQLNAQRKLLGIELTTLEMQTGISSSTLKRLFKDPSQVKFQTICIVAETLGFNLCAIN
                     THKQE"
     gene            complement(424..1443)
                     /gene="glpX"
                     /locus_tag="PXB27_002527"
                     /pseudo
     CDS             complement(424..1443)
                     /gene="glpX"
                     /locus_tag="PXB27_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667633.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
BASE COUNT          445 a          351 c          263 g          406 t
ORIGIN      
        1 tctactttta gctcaaaaat aaaatgaaac tatcaagcga actacaaaat cagataaacc
       61 aactaaatca gttacgaaaa caggcactca aacaagagca agacgagcag aaattgtttg
      121 aaaaaaataa aattgaagat ttgccacatt ttggcacgca gcttaatgct cagaggaaac
      181 tattaggcat agaattaacc acccttgaaa tgcaaaccgg catttcaagc tctaccctaa
      241 aacgcttatt taaagaccca agccaagtga aatttcagac aatttgtatt gtggcagaaa
      301 cactcggctt taatttatgt gcaattaata ctcacaaaca agagtaagcg tgacaatgta
      361 gtgattttga ttactttggg aagacaaaaa atcgggctaa aagcccgatt tatcagagaa
      421 aagctataaa tccaataaag aaaggagttc gttatcttta cgatcgacat aatggtcgga
      481 ttgaatttta cgaatggttc tagatttgcc acgaataagt aaggtttcag tgctggcaat
      541 attgcctcta cgtttaatcc cttttaacaa ttcacctgaa gtaatgccgg ttgcaacaaa
      601 gacgatattg tcatcacgca ccaaatcttc taatttcaac accgtattca ccggtacatt
      661 catttcatta cagcgtaaaa gctctttttc ggtagcagtt aaattttccg ggctattccc
      721 cttcacttgg ttgcgaggaa ttaaacgtgc ttgcatatcg ccacccaatg cacgaaccgc
      781 agcccccgcc actacccctt caggtgctcc gccgatacca taaagcagat ccaattcgct
      841 ctctggcaaa cagcattgca ccgcagccgc aacatcgcca tcaggaatcg caatcactct
      901 tacccctaat ttttgcacat cggcaattat tttttcgtgg cgaggcttag caagatagca
      961 atggttaatt gggaaagtaa tttgcccttt ttcgaggcga cacggcgaag attttgttca
     1021 agcggaagat ttaaatcaat cattcctttg cattcctgcc ccaccactaa tttttccata
     1081 tacatatcag gggcttttaa aaaggtttct ttgccgcctg ctgctaatac cggcaacgca
     1141 tttgcctgcc ccattgcagt catacgagta ccgtcaatcg ggtcaaccgc aatactgatt
     1201 tcctcatctt ctaggcggga tagaccgatt ttctcgccaa tataaagcat cggtgcttcg
     1261 tcaatttcgc cctcaccaat taccacttcg ccacggattt ccatttggtt cagcataaac
     1321 cgcattgctt ttcaccgcag cttcatcagc ggcattttta tccccacggc ctagccaaga
     1381 gtaagctgct agagccgctg cttccgtaac acgagaaaat tcaaaagaaa gagttcgttt
     1441 catttttgtt ccttagtttc ttgaa
//
LOCUS       DANLUQ010000245         1456 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.247, whole genome shotgun sequence.
ACCESSION   DANLUQ010000245 DANLUQ010000000
VERSION     DANLUQ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.247"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            13..1293
                     /locus_tag="PXB27_002528"
     CDS             13..1293
                     /locus_tag="PXB27_002528"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005762304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HDL5347260.1"
                     /translation="MSKATLTLETGEKLELNMQKGTLGYQTIDIQPFLKHKLFAYDPG
                     LVSTAVCRSNITYVDGDEGVLLYRGYPIDQLAKHSDYLEVGYMLLFGERPTKEEYKDF
                     VQLIKKHTLVHEQLTKFFSGFRRDSHPMAVMCGVSGALAAFYHDAIDVNNQAHRELTA
                     IRLLAKIPTLAAMCYKYSIGQPFMFPQNHLSYAGNFLYMMFATPCEPYEVNPVLERAL
                     DRIFILHADHEQNASTSTVRTAASSGANPFACIAAGIASLWGPAHGGANEACINMLKE
                     IGTVDRIPEFIARAKDKNDPFRLMGFGHRVYKNYDPRAKVMRETCHEVLKELNIDNPL
                     FEVALELERIALSDPYFIEHKLYPNVDFYSGIVLKAIGIPTSMFTVMFALARTVGWIA
                     HWKEMYLQGNIKIVRPRQIYTGHTKRDFEPMDKS"
BASE COUNT          429 a          337 c          281 g          409 t
ORIGIN      
        1 taggagtaag ctatgtcaaa agcaacatta acactcgaaa caggtgaaaa acttgaactc
       61 aatatgcaaa aaggaacctt agggtatcaa acgattgata ttcagccttt cttaaagcat
      121 aagttatttg cttatgatcc cggattggtt tcgaccgctg tatgccgatc caatatcact
      181 tatgttgatg gcgatgaagg ggttttactc tatcgaggct acccgattga ccaattagcc
      241 aaacattctg attacttaga agtcggttat atgctgttat tcggcgaacg ccctaccaaa
      301 gaagaataca aagacttcgt tcaactcatc aaaaaacaca ctcttgtaca cgaacaacta
      361 accaaattct tctccggttt ccgccgagat tcgcacccaa tggcggtgat gtgtggggta
      421 agtggagctt tggctgcctt ctaccacgac gccattgatg taaacaacca agcccaccgt
      481 gaattaaccg ccattcgttt gttagccaaa atcccaaccc ttgcggcgat gtgttataaa
      541 tactctatcg gtcagccgtt tatgttccca caaaaccact tatcttacgc cggcaacttc
      601 ttatatatga tgtttgccac tccttgtgaa ccgtacgaag tgaaccctgt gttagagcgt
      661 gcgttagacc gaattttcat tttacatgca gaccacgaac aaaacgcttc gacctcaacc
      721 gtgcgaaccg ccgcttcatc aggtgcaaac ccatttgctt gcattgctgc cggtattgcc
      781 tctctctggg gacctgcaca cggaggagca aatgaagcct gtatcaatat gttgaaagaa
      841 atcggcactg tggatcgcat tcctgaattt attgctcgag ccaaagataa aaatgacccg
      901 ttccgcttaa tgggctttgg gcatcgtgtc tataaaaact acgaccctcg tgcgaaagta
      961 atgcgtgaaa cctgccacga agtgttaaaa gaactgaaca ttgataaccc actatttgaa
     1021 gtggcattgg aattagaacg tattgcgtta agcgaccctt atttcattga gcataaactt
     1081 tatccaaacg tagatttcta ctccggaatc gtgctaaaag ccatcggtat tccaacctca
     1141 atgtttaccg taatgtttgc cctagccaga accgttggct ggattgctca ctggaaagag
     1201 atgtatctcc aaggcaatat caaaatcgtt cgtccacgtc aaatctacac cggacatact
     1261 aaacgtgatt ttgagccaat ggataaatca taagattggc aaaaaatcga aaaattttaa
     1321 ccgcttgttt ccacaagcgg ttatattttg cataaaattt gcaaatcagg ctaatttatt
     1381 tagcagttgt gttctaacat cttgaccatt ttcatcgctt tttacagcca taataaataa
     1441 gccttttcct tgactt
//
LOCUS       DANLUQ010000246         1451 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.248, whole genome shotgun sequence.
ACCESSION   DANLUQ010000246 DANLUQ010000000
VERSION     DANLUQ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.248"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(108..183)
                     /locus_tag="PXB27_002529"
     tRNA            complement(108..183)
                     /locus_tag="PXB27_002529"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(148..150),aa:Ile,seq:cat)
     gene            complement(277..1014)
                     /gene="pgeF"
                     /locus_tag="PXB27_002530"
     CDS             complement(277..1014)
                     /gene="pgeF"
                     /locus_tag="PXB27_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005616864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HDL5347261.1"
                     /translation="MDKIIPNWNVPKHIHALTTLRTGGVSKPPFDSFNLGDHVNDEPQ
                     DIAQNRALLVEKFQLPQAPLFLTQTHSTRVIELPYSGDNIKADAIYTNQPNQVCLVMT
                     ADCLPVLFASQDGSEIAAAHAGWRGLCDGILEATVEKFNCPPHEISAWLGPAIGPNAF
                     QVGSEVADQFCAFDPRAKEALIEDSTTSGKFLGNLYQIATQRLNKLGITAISGGEYCT
                     YSQPELFFSYRRDKQTGRMATLIWRTE"
     gene            complement(1046..1432)
                     /locus_tag="PXB27_002531"
     CDS             complement(1046..1432)
                     /locus_tag="PXB27_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015432850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDL5347262.1"
                     /translation="MTVIHTENAPAAIGPYVQAVDLGNMVLTSGQIPVNPETGEIPSD
                     IVQQTRQSLNNVKAIIEQAGLTVADIVKTTVFVKDLNDFAKVNAEYQAFFQENEHPNF
                     PARSCVEVARLPKDVGIEIEAIAVRR"
BASE COUNT          392 a          302 c          336 g          421 t
ORIGIN      
        1 ctaagataca atgcaactaa agaagacaat aagagactat aaaagaatct aagttcttga
       61 ttttatagta aatttgagac aatgaaagac aatatgcaac gcttagatgg tccccccagc
      121 cggacttgaa ccagcgacca agcgattatg agtcgcctgc tctaaccact gagctatggg
      181 gggatttgaa aagtggttgg aattatagag aaaaaccgag tagtcgtcta gaggcagaag
      241 attactcggt taattattga acgagagaaa atagggttat tctgttcgcc aaattaaggt
      301 tgccattcgc ccggtttgtt tgtctcggcg gtaggaaaaa aacagttctg gttggctgta
      361 ggtgcaatat tcaccgcctg aaatggcggt aatgcctaat ttattcagac gttgggtggc
      421 aatttggtaa agatttccta aaaatttacc gcttgtagtg ctgtcttcaa taagggcttc
      481 tttcgctctt gggtcaaaag cacagaattg gtctgccact tcactcccga cttgaaaagc
      541 attcggtccg atagccggcc ctagccaagc tgatatttcg tgtggcgggc agttgaattt
      601 ctctaccgtc gcctctaata tgccatcgca taacccacgc cagccggcgt gtgccgccgc
      661 aatttccgac ccgtcttggc tggcaaataa taccggcaaa caatctgcag tcatcactaa
      721 gcagacttgg tttggttgat tggtgtaaat cgcatcggct ttaatattat cacctgaata
      781 gggaagttca atcactcggg tgctgtgagt ttgggttaaa aacagcggag cttgtggtaa
      841 ttgaaatttc tcaaccaata aagcacgatt ttgagcgata tcttgtggct cgtcattaac
      901 gtgatcacct aagttgaaac tgtcaaaagg cggtttgctt actccgcctg ttcttaaggt
      961 cgtcagtgcg tgaatatgtt taggcacatt ccaatttgga ataattttat ccatttgcaa
     1021 aaaatctcaa gaaaatgacc gcttgttatc ggcgtactgc aatcgcttcg atctcaatgc
     1081 caacatcttt tggtaaacga gccacttcta cgcaagaacg agccggaaaa ttagggtgtt
     1141 cgttttcttg gaagaaggct tggtattccg catttacctt tgcgaagtcg ttaagatctt
     1201 tgacaaatac ggtggtcttt acaatatcgg caacggttaa gccggcttgt tcgataatgg
     1261 ctttcacgtt gttcagagat tggcgggttt gttgcacaat atcactcggg atttcgccgg
     1321 tttcaggatt cacgggaatt tgcccagaag ttaaaaccat attgcctaaa tcaactgctt
     1381 gcacataagg cccaatcgct gccggtgcat tttctgtgtg gataactgtc atcttgtgtt
     1441 cctcttaatg a
//
LOCUS       DANLUQ010000247         1446 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.249, whole genome shotgun sequence.
ACCESSION   DANLUQ010000247 DANLUQ010000000
VERSION     DANLUQ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.249"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            33..1349
                     /gene="argA"
                     /locus_tag="PXB27_002532"
     CDS             33..1349
                     /gene="argA"
                     /locus_tag="PXB27_002532"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HDL5347263.1"
                     /translation="MQNTELALAQWFRQSTPYVNMHRGKTFVIMLDGDVIESPNFVNI
                     INDISLLHSLNIKLVIVFGARPQINQLLKQNHIEPEYYHNIRVTNPQSLEYVKQAVGK
                     VHYDFFARLSLRLPHSEVINVVSGNMILAQPIGVVDGVDYTLSGKIRRINIESIKQQL
                     AQNSIVLLGPIAPSVTGEMFNLPFEDIATQVAIKLRADKLIGFCEEQGILDENGKPIP
                     DYLPQDGETLLQQLIERGEYHTPKARFLQAAITVCREGVKRSHLLSYKEDGSLLQELF
                     SRDGIGTQFSMESSEQVRIANSSDIPGLLDLIRPLEQQGILVKRSREQLEMEITNYTI
                     IERDGVVIACAALNPYPEEKMAEMACVAVHPDYRDSSRGDILLENIQKRAVQLGMEKL
                     FVLTTRTTQWFQERGFRLAGAQDLPRYKRENYNYQRRSKVLILPLW"
BASE COUNT          446 a          272 c          321 g          407 t
ORIGIN      
        1 taatatgcgt ttatattata aagaggaaat atatgcaaaa cacagaactc gcattagctc
       61 aatggtttag gcaatctaca ccttatgtta atatgcacag aggcaaaacc tttgtgatta
      121 tgcttgatgg cgatgttatc gaaagcccga attttgtcaa tattatcaat gacatcagtc
      181 tcctgcacag cctgaatatc aaattagtga ttgtatttgg ggcaagaccg caaattaacc
      241 aattgttaaa acaaaatcat attgagccgg agtattacca caatatccgt gtgactaatc
      301 cacaatcgct agaatatgtg aaacaagcgg tagggaaagt tcattatgat ttctttgccc
      361 gcctttcttt gcgtttaccc cattctgaag tgattaatgt agtcagtggt aatatgattt
      421 tagctcagcc gattggcgtg gtggatgggg tagattatac actaagcggt aaaatccgtc
      481 gtattaatat tgagagcatt aagcagcaac tggcacaaaa ttcgattgta ttacttggtc
      541 cgattgcccc gtctgttacc ggtgaaatgt ttaatctgcc gtttgaagat attgccacac
      601 aggttgcaat taaacttcgt gccgataaat taatcggttt ttgtgaagag caggggattt
      661 tagatgaaaa cggcaagcca attccagatt atcttccgca agatggcgaa accttgttac
      721 agcagttaat cgagcgtggc gaatatcaca ccccgaaagc tcggtttttg caggcggcga
      781 tcacggtatg ccgagaggga gtgaaacgct cgcatttatt aagctataaa gaagatggct
      841 cattgcttca agagttattc tctcgtgatg gtattggcac acagttctca atggaaagct
      901 cggaacaggt acgtattgct aattccagtg atattccggg attattggat ttaatccgcc
      961 cacttgaaca gcagggcatt ttagtcaaac gctctcgaga acaattagaa atggaaatca
     1021 caaactacac cattattgag cgtgatggtg tggtgattgc ttgtgcggca cttaacccgt
     1081 atccggaaga aaaaatggcg gaaatggcgt gtgtggcggt tcaccctgat tatcgtgatt
     1141 cctcaagagg ggatattcta ctggaaaata ttcaaaaacg ggcagtgcaa ctgggaatgg
     1201 agaaactctt tgtgctgact acaagaacca cacaatggtt ccaagagcga ggatttaggc
     1261 tggcaggtgc ccaagattta cctcgctata aacgggaaaa ttacaattat caacgtcgtt
     1321 ctaaagtatt aattttacca ctttggtaaa atattcaatg tttttgaccg cttgttacca
     1381 agagaactat aaaaggagaa ctatgaaaaa acatcaaccc atcaaagggg ctatggcaat
     1441 tattac
//
LOCUS       DANLUQ010000248         1445 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.250, whole genome shotgun sequence.
ACCESSION   DANLUQ010000248 DANLUQ010000000
VERSION     DANLUQ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.250"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            371..556
                     /locus_tag="PXB27_002533"
                     /pseudo
     CDS             371..556
                     /locus_tag="PXB27_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249206.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1197..1445
                     /locus_tag="PXB27_002534"
     CDS             1197..1445
                     /locus_tag="PXB27_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347264.1"
                     /translation="MISGEKMTWESLGASAIGGAIGGGIGATMKAGAVTASTLSALGG
                     GMGEGLYKSTSQGIKNSIGIKASCNQSGSDYGDGCDYQ"
BASE COUNT          477 a          173 c          316 g          479 t
ORIGIN      
        1 gattaaaggg agaatgaaat gaaagaacca tcaattttac aattaagctt agtagttgga
       61 gggaattctg aaaataaggc taatcaacca gatcttgtat cttatggcgt tgcttttgct
      121 acagggtttg caagaggggg gctttaggcg gtttatccag tacttctgtt aatttagccc
      181 ttgatttagc gaggagccta cctattacaa tacatgaaaa ttctttacct agcggtgtaa
      241 aagaattaat tccatcatct ttaccaataa agcctacgtt tggagttcca ggtagaacga
      301 ataataacca atctggctca aactacgatt gtggtgatgg tacagattat caaggctagt
      361 ggagatgaaa atgaaaattc aatttccaat ttcttatcaa gaatttcgtg aaagttattc
      421 tgagaaacaa ccacttttaa tgaaaggtgc tatagagtag caagatttac tttcttggaa
      481 ggctatcaat gaagtattac ctcgttgtga tttactttct gaagatgcaa ttaaagtaat
      541 gtataaagta agttgaaata ctttaatgga gaaagtatgg tggatttttc tattttgata
      601 ttagttatag tattgcctgt tatttttgtg aaagttatag atgtactgtt gcttagtaag
      661 ttagatatga gtaaggaaaa aaatgacaaa tgccaaataa taatatattt atttataata
      721 attttacttg gggcatataa gagctatttt attcaaaaat aaataagagg agagaaaatg
      781 aaagatctta gtattaagga ttgtgaaata atatatggag gcatagcccc tttgtggtgg
      841 gatggtactg gcattacatc atactgggaa ctaaattata ccaccctacc actagtattt
      901 tagtttatac ggctagataa atatgatttt tgaaaaaata ttacttatat tgattatccc
      961 attcatatta tcaagaattg tgtctattct tcttgtaaag aagattgtaa aaaataaaaa
     1021 tgtacaagat gttattgagg ctatagttct aatagtattt gttgtaatta taaacatcat
     1081 agaggaatat ctcttatgaa agagttaaaa attgaggatt gccaatttgt ttcggcaggc
     1141 ttgcacttgt tattgtagga gctgttgctg gagctgcata ttatgttggg aatggtatga
     1201 tttctgggga aaaaatgact tgggagagtt tgggagcatc agctataggt ggggctattg
     1261 gtggcggtat tggagcaaca atgaaagctg gtgcggtgac agcgtcaacc ttatctgctt
     1321 taggtggtgg tatgggggag ggcttgtata aaagtacttc tcaaggtatc aaaaatagta
     1381 ttggtataaa ggcgagttgc aatcaatcag gttcggatta tggtgatgga tgtgattatc
     1441 aataa
//
LOCUS       DANLUQ010000249         1399 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.251, whole genome shotgun sequence.
ACCESSION   DANLUQ010000249 DANLUQ010000000
VERSION     DANLUQ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.251"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            115..1185
                     /locus_tag="PXB27_002535"
     CDS             115..1185
                     /locus_tag="PXB27_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347265.1"
                     /translation="MKFNKKLILTFAATLVLSACGGSGSGGSSSTPNHPKPVLVPKTQ
                     NNLQAQNVPQAQNASQAQNAPQAQNAPQAQNAPQVENAPQAQNAPQVENAPQAEVTPP
                     VPQPQSQKIDGSFDKIGSVKLNKEAQTLELSRFTLVDKLGTPPKFDKVSGKKIIEEKD
                     FLVLNLSDINAEQLSGDFLIRRSDDLFYGYYHDTNGKNLVDAADKFSQYFVVYDEKRV
                     NDNISDKLTATYRKKEGFVYGSNPHTKEFAARISKLGDVEIKFENGQAQGSIKDEKDG
                     NAEIFTIKGDTKQLEITPTESNRIIIAILDQNQKSYTPGMEKAIMETKFIDSKAGNSD
                     QKYLIGEAKSDNWQAIMVSEKK"
BASE COUNT          505 a          261 c          249 g          384 t
ORIGIN      
        1 attttcataa gggaaacgtt tacgtaaaac tcctcagacc actcattctt attttatata
       61 aaaaatgtga tagacttctc gcagtttcgt tttatatatt taaggaataa ctaagtgaaa
      121 ttcaataaaa aattaatttt aacatttgct gcaaccttag ttttaagtgc ttgcggagga
      181 agcggtagcg gaggttcgtc ttcaacaccg aatcacccca aaccagtact agtaccaaaa
      241 acacaaaata atcttcaagc acaaaatgtt cctcaggcac aaaatgcctc tcaggcacaa
      301 aatgcccctc aggcacaaaa tgctcctcag gcacaaaatg ctcctcaggt ggaaaatgct
      361 cctcaggcac aaaatgctcc tcaggtagaa aatgctcctc aagcagaggt tactccgcct
      421 gtaccacagc cacaatcaca aaaaattgac ggttcttttg ataaaattgg ttcagtaaaa
      481 ctcaataaag aggctcaaac tcttgagctt agtagattca ctttggtgga taaattaggc
      541 acaccaccga agtttgataa agtaagcggt aaaaaaatta ttgaagaaaa agattttctc
      601 gtattaaatt tgtctgatat taatgctgaa caactctctg gcgattttct tattcgccgt
      661 agcgatgatc tattctatgg ctactatcac gatacaaatg gcaaaaatct tgtcgatgct
      721 gccgataaat tcagtcaata ttttgtcgtg tatgatgaga aacgggtaaa tgataatatc
      781 tctgataaat taacagcaac ttaccgtaaa aaagaaggct ttgtatatgg ttcaaatcca
      841 catactaaag aatttgccgc acggatcagc aaattggggg atgtagaaat taaatttgaa
      901 aatggtcaag ctcaaggaag tataaaagac gaaaaagatg gaaatgctga gatctttact
      961 attaaaggtg atacaaaaca gttagagatt accccaacgg aaagtaaccg aatcattata
     1021 gcaattttag accaaaatca aaaaagctat actccaggaa tggaaaaagc aattatggaa
     1081 actaagttta ttgattcaaa ggctggtaat tccgaccaaa aatacttaat cggtgaagca
     1141 aaaagcgata actggcaagc aataatggtt agcgagaaaa aataaagtta tcttttgcta
     1201 aaaactgaaa taaaaaggct gagtccgggt aatatcggcc tcagtctttt aaattgtaga
     1261 aaatcatctg tagaagatca aacctaatct gacagtcccc cgttttaaat taccgtgtct
     1321 gtcagattaa tttgagctta aattcttttc cacccaaatc cgttttccat caagtaaggt
     1381 tgccatcggt gttctgcca
//
LOCUS       DANLUQ010000250         1377 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.252, whole genome shotgun sequence.
ACCESSION   DANLUQ010000250 DANLUQ010000000
VERSION     DANLUQ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.252"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(164..295)
                     /locus_tag="PXB27_002536"
                     /pseudo
     CDS             complement(164..295)
                     /locus_tag="PXB27_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250081.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            complement(316..516)
                     /locus_tag="PXB27_002537"
     CDS             complement(316..516)
                     /locus_tag="PXB27_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL5347266.1"
                     /translation="MNLTSYLSDRPRGFKADFAKKLGISKSYLRQVETGYSPMPAYLA
                     KKIEEVTNGEVAKSELRPDLWD"
     gene            647..1330
                     /locus_tag="PXB27_002538"
     CDS             647..1330
                     /locus_tag="PXB27_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005714533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL5347267.1"
                     /translation="MIKGMTLPEIRRKKLTEWFNTRDIPEKEKSYISQLKSGKSSFGE
                     RAARRLENEYGMPSFYLDYDEETESAIVSAMSIQETQSHQYPIHLIDFKAKAGETGFV
                     NTNYPEIIQSIYFSLDGLLEIVGRKSSNGIEMITIPTDSMSPTINKGDVVFIDTTINY
                     YDNEGVYIFVIDDEVYIKRLQKIPGGIYRALSDNKTYEPFDIKRETLDTAIILGKFIR
                     VLPINPKDL"
BASE COUNT          463 a          242 c          257 g          415 t
ORIGIN      
        1 ctattgagcc accgtgaata gcggttacac cggcattttg ccaagggcta ggctgagtga
       61 tatctgtaac attatcacta gcctcctcaa gcattagcct acgagcaaat aaaaatggat
      121 tagctttacc ttgaatatta tctaatcgta atgcgaaacg aatatccgca agctcaatac
      181 ttaagtaatc aataatcacc gccaccattt ccacattatc tgcaaatgta cggctaaatt
      241 gtaattcact cagtccgatt gctcttgcat tttttgttaa ttcattgcgt ggcattgcac
      301 attcctttgg gtaaattaat cccacagatc ggggcgaagc tcagatttgg ctacttcgcc
      361 attcgtgact tcttcaattt tcttagcaag ataggctggc attggtgaat accctgtttc
      421 aacttgtcgt aaataagatt tggaaatacc aagctttttt gcaaagtcag ctttgaaacc
      481 acgagggcga tctgacaaat aactagttaa gttcattaac actcccgtta agtaaaaatt
      541 agattgagtt tagatttaac taaatacaaa gtcaagtaaa gtttagcttt tgcttgttta
      601 ggaaattcta aataaaataa tttaaaaatg aaaagtgagg ttaattatga ttaaaggtat
      661 gaccctgcca gagattcgca gaaaaaaact tacagaatgg ttcaacacaa gagatattcc
      721 agaaaaggaa aaaagctaca tatcacaatt aaaatcagga aaatcatctt ttggtgagcg
      781 tgcagcaaga agattagaga atgaatatgg aatgcctagc ttttatttgg actatgatga
      841 agaaacagaa agtgcgattg tgtctgctat gtcaattcaa gaaacacagt ctcatcaata
      901 ccccattcat ctaattgact tcaaagctaa ggcgggcgaa acaggttttg tcaatactaa
      961 ctatcctgaa attattcaat ctatctattt ctctttagac ggcttgcttg aaattgtagg
     1021 gcgaaaatca agcaatggca ttgagatgat caccattcca actgatagta tgagcccaac
     1081 aataaacaaa ggggatgttg tttttattga tactacaatc aattattacg ataacgaggg
     1141 tgtttatatt tttgttattg atgatgaagt ttatataaaa agactacaaa aaattccagg
     1201 tggtatttat agagccctat cggacaacaa gacttacgaa ccttttgata taaagagaga
     1261 aaccttagat actgctatta tccttgggaa atttattcga gtattgccaa taaatcctaa
     1321 agacttgtag gcattagcgt agcggttgag catcggagct atttgtagcc agcggtt
//
LOCUS       DANLUQ010000251         1354 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.253, whole genome shotgun sequence.
ACCESSION   DANLUQ010000251 DANLUQ010000000
VERSION     DANLUQ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.253"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..1285
                     /locus_tag="PXB27_002539"
     CDS             <1..1285
                     /locus_tag="PXB27_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HDL5347268.1"
                     /translation="LLLGVLTSLLSISGSNQAFANWAQKKVKGKRGAKLMAAGLVFFT
                     FIDDYFHSLAVGAIARPVTDKFRVSRAKLAYILDSTAAPMCVLMPISSWGAYIITLIA
                     GLLATHSITGYSPLGAFMTMSAMNFYAIFAMIMVFFVAYFSFDIGSMARFERQAAHLA
                     ELDDEQIETKGHVRDLILPIIALIVATVSMMMKTGADALAESGASFSILGAFENTTVG
                     ISLVVGGVAGLIVATICIITSGNISFKNYVRAYGLGVKMMSGAITILFFAWTINGVVS
                     DMQTGKYLSGLVAESIGAEFLPAILFVLAAAMAFSTGTSWGTFGIMLPIGASMAVHSD
                     PALIIPCLSAVMAGAVCGDHCSPVSDTTILSSTGAQCNHMDHVISQLPYALLVATASI
                     IGYLVVGFTQSAVLGFIATAVVMAILILLFKEKKH"
BASE COUNT          357 a          232 c          306 g          459 t
ORIGIN      
        1 gcttttatta ggggtattaa cgtcattatt aagtatttca ggtagtaatc aggcgtttgc
       61 gaattgggcg cagaaaaagg taaaaggcaa acgaggcgca aaattaatgg cggcaggttt
      121 ggttttcttt acctttattg atgactattt ccacagttta gcagtaggtg cgattgcccg
      181 ccctgttacg gataaattcc gtgtttctcg tgctaaatta gcttatattt tagattctac
      241 cgccgctcca atgtgcgtgt taatgccaat ttcaagctgg ggggcttata ttattacctt
      301 aatagctggc ttattggcta cgcattccat cacaggctat tcgccactag gcgcatttat
      361 gacaatgagc gcaatgaact tttacgccat ttttgcgatg attatggtgt tctttgtggc
      421 atatttctca tttgatattg gttcgatggc gcgttttgaa cgtcaagcag cgcatttagc
      481 agaattagat gatgagcaaa ttgaaacgaa aggacacgtg cgcgatttga ttttgccaat
      541 tattgcactt attgttgcta cagtctcaat gatgatgaaa acaggtgcag atgcactggc
      601 cgaatcaggt gcttcatttt ctatactagg tgcttttgaa aatactacgg taggcatttc
      661 attagttgtt ggtggtgtgg cagggcttat agttgcaact atttgtatca ttacaagcgg
      721 taatatttct tttaaaaatt acgtccgtgc ttatggctta ggtgtgaaga tgatgtctgg
      781 tgcaatcact attctcttct ttgcgtggac aataaacggc gtagtaagtg atatgcaaac
      841 agggaaatat ctatcaggat tggttgcaga aagcattgga gcagagtttt tacctgcaat
      901 tctatttgtg ttagccgcag caatggcgtt ttcaacagga actagctggg gaacatttgg
      961 tattatgttg ccaattggag cctcaatggc agtgcattca gatccagcat taattattcc
     1021 ttgtttatct gcagtaatgg ctggtgcggt ttgtggtgat cactgttctc cagtatctga
     1081 taccactatt ctttcttcaa caggcgcaca atgtaatcat atggatcatg tcatttctca
     1141 attaccttat gctttattag ttgcaacagc ttctattatt ggttacttag tggttggctt
     1201 tacccaatca gcggtattgg gatttattgc tacagctgtt gttatggcaa tactcatttt
     1261 gctttttaaa gaaaaaaagc attaatttcc gaaaatgtga agggaatcac agaatgagat
     1321 ttattgatga gataaaatag cactcaagat atgg
//
LOCUS       DANLUQ010000252         1347 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.254, whole genome shotgun sequence.
ACCESSION   DANLUQ010000252 DANLUQ010000000
VERSION     DANLUQ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.254"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            322..576
                     /locus_tag="PXB27_002540"
     CDS             322..576
                     /locus_tag="PXB27_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347269.1"
                     /translation="MICHADPEYVKYNYAGLWEKGSDGTFFCNYQQFMPEVEAKESLR
                     KQEPELDDRYNEEEYSEEEYPDENDASDENDNYDETYNVF"
     gene            776..952
                     /locus_tag="PXB27_002541"
     CDS             776..952
                     /locus_tag="PXB27_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347270.1"
                     /translation="MATSSFDKRFVVKDPKAVKVLIEALQSPIKVVLPKKDLEKEEKE
                     ALCAIKKRLASMGL"
     gene            913..>1347
                     /locus_tag="PXB27_002542"
     CDS             913..>1347
                     /locus_tag="PXB27_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347271.1"
                     /translation="MCNQKTLSLYGLVKELGIEKTQLFLEQAFSCSRNKDVESFLLNK
                     AIRFETALAAATHLIINEQGDILAYFTLSFKEIEVEVSKSQLKKLTAGLSNDSHLRVL
                     LIGQIGKNEKVIDNPAKLPDILNHIYAQLYKAQRVLGGRVVIL"
BASE COUNT          494 a          237 c          231 g          385 t
ORIGIN      
        1 ccgctactca acatacaggg atggttatct tgataacgct cacgatactc ttgagcaata
       61 gctagtaatt gctgattagt catataaagt ctccaataac ataataaacc tgaaaacgtt
      121 cttagtattc tggcaacatt tttataaata gaaaccaaaa actatattca aatcattttt
      181 agtttctgct caacattcct atgtttttcc atacactgaa gtaaatttac ctaaccctat
      241 tttgctgtga aaccaaaact cctcacgctt atccttagta gcatttcgat gtcgctcact
      301 tatgccaccc ctcaattagg gatgatatgc cacgctgatc ctgaatatgt aaaatacaac
      361 tacgccggac tttgggaaaa aggcagcgat ggcacttttt tctgcaatta ccagcaattt
      421 atgcctgaag ttgaagctaa agaatcacta aggaaacaag aaccagaact tgatgataga
      481 tataacgaag aggaatatag cgaagaagaa tatcctgatg aaaatgatgc cagcgatgaa
      541 aacgataact acgatgaaac ttataatgtt ttctagtatg tatatggaaa ccggaggaat
      601 ccgtgattga aaaggcttgc atttccaaat atagtctata tattgtggta attgaaagta
      661 tttctatgct aacaagcata aggctatgta atcgaaaacc acccacaagg cctaaataca
      721 gtattattac agccatgagt aaccatggta atttaaatat ttaaggagtt ctataatggc
      781 aacatcatca tttgataaac gctttgtagt caaagatcca aaagctgtca aggtattaat
      841 tgaggcatta caaagcccaa tcaaagtagt tttacctaaa aaagacttag aaaaagaaga
      901 gaaagaagca ctatgtgcaa tcaaaaaacg cttagcctct atgggcttgt aaaagaacta
      961 ggtattgaaa aaacacaact ctttttggag caagcatttt cttgcagtag aaataaagat
     1021 gttgaaagtt ttctacttaa taaagcaatc agatttgaaa ccgcattagc agcagcaaca
     1081 catttaatca ttaatgaaca gggcgatatt ttggcttact ttaccctctc ttttaaagaa
     1141 attgaggtgg aagtaagcaa aagccaatta aaaaagctaa ctgctggttt aagtaatgat
     1201 tcacatttaa gagtattgct tatcggtcaa atcggcaaaa atgaaaaagt aattgacaat
     1261 ccagccaaat tgccagatat tctaaatcat atctatgcac aactttataa agcacaacga
     1321 gttttaggtg ggcgagtcgt tatatta
//
LOCUS       DANLUQ010000253         1303 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.255, whole genome shotgun sequence.
ACCESSION   DANLUQ010000253 DANLUQ010000000
VERSION     DANLUQ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.255"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(46..585)
                     /locus_tag="PXB27_002543"
     CDS             complement(46..585)
                     /locus_tag="PXB27_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005566788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347272.1"
                     /translation="MAVSAYIGIPGSGKSYEVVKSVILPAVASNRRVVSNIYGLNKEK
                     IHQYLLRKNKKLMIEELGEVIYVTNEQCLESDFLPSMDEQDSFCKAGDLIIIDEVWRI
                     WGNDKDIPKNHRSFIAEHRHFADPITGVTCDLVVINQDISQIPRFIKEGLRQLTECKN
                     TLVWGCVIVIGLMFFKERI"
     gene            complement(595..888)
                     /locus_tag="PXB27_002544"
     CDS             complement(595..888)
                     /locus_tag="PXB27_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HDL5347273.1"
                     /translation="MFKVIFMALSAIVNYLISDFLIKGVIFLSVYFLTSELLPVVIEM
                     LVSRKLIPDLNALFGQLPESMWYFLNIFQIPMAISLMISAMISRFVRRMPFIG"
     gene            complement(897..1097)
                     /locus_tag="PXB27_002545"
     CDS             complement(897..1097)
                     /locus_tag="PXB27_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347274.1"
                     /translation="MLPDLQNFELNKRTAQCPVWTFNIEYLRFHYELREHCDLIEPHK
                     EILKLLFGIIWAFIALRYVLSA"
BASE COUNT          462 a          250 c          175 g          416 t
ORIGIN      
        1 ttttatcata tttttcttga taagaatttg tccggttctt tttagctaaa ttctttcctt
       61 gaaaaacatc aacccgataa cgattacgca accccaaacc aacgtgtttt tgcattcggt
      121 aagttgtctc aatccttctt taataaatct tggaatttga gaaatatctt gatttattac
      181 gactaaatca caagttacac ctgttatagg atctgcaaaa tgtcgatgtt cagcaataaa
      241 tgaacgatga ttttttggaa tatccttgtc gtttccccaa atacgccaaa cttcatcaat
      301 aattatcaag tccccagctt tgcaaaatga atcttgttcg tccattgatg gtaaaaaatc
      361 cgattccaag cattgttcat tcgttacata tatgacttca ccaagctctt caatcattaa
      421 ttttttattt ttacgcagta aatattgatg aattttctct ttatttaatc cataaatgtt
      481 ggaaacaact cttcgattcg aagcaacagc aggtaaaatt actgacttaa ccacttcata
      541 cgatttgccg cttccaggaa tccctatata cgctgaaaca gccatagcac tcctctatcc
      601 aataaatggc atacgtctaa caaaacgtga aatcattgct gaaatcatta aagaaatagc
      661 cattggtatc tgaaaaatat ttaaaaaata ccacatactt tcaggcaatt gcccaaataa
      721 cgcatttaaa tcaggtatca attttctaga cacaagcatc tctattacta caggaagtag
      781 ttcagaagtt aaaaaataga cagataaaaa tatgacaccc ttaattaaaa aatctgaaat
      841 caaataattc acaattgctg atagtgccat aaaaatcact ttaaacattt atcctcctaa
      901 gcacttaata cataacgtaa agcaataaac gcccaaataa tgccgaataa tagctttaag
      961 atttctttat gtggttctat caaatcgcaa tgttctctaa gttcataatg aaatctcaag
     1021 tattcaatat taaatgtcca aacagggcat tgtgcggttc gtttattcag ttcaaaattc
     1081 tgtaaatctg gtagcaattt tttaaaaggg gatataattg attccgccgt aggaacttcc
     1141 agatcaggca aattaatatc aggtgtagaa gatcagactt gatctgacaa ttcactctaa
     1201 aaagtgagag tttcccgttt agaatatgag tgtcaaaaat caatctaaac aaaaaggaaa
     1261 ctctcatgtt ttattctaac aaccctctca ttaaacacaa gac
//
LOCUS       DANLUQ010000254         1150 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.256, whole genome shotgun sequence.
ACCESSION   DANLUQ010000254 DANLUQ010000000
VERSION     DANLUQ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.256"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            28..564
                     /gene="proQ"
                     /locus_tag="PXB27_002546"
     CDS             28..564
                     /gene="proQ"
                     /locus_tag="PXB27_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HDL5347275.1"
                     /translation="MSEQIEQKVEQTQNKTNPSTKEVIAYLAEKFPLCFTLEGEVKPL
                     KVGLFQELAEALKDDEKISKTLLRQVLRNYTNSWRYLAASQANVARVGLQGEEVGVVT
                     EDEATHAAEALAAAKAVVAERKAQERKAQRKEYFKQKAREENAKKRAEFKAKKAIDNL
                     PKASEEKLAALADKFGRK"
     gene            650..>1150
                     /locus_tag="PXB27_002547"
     CDS             650..>1150
                     /locus_tag="PXB27_002547"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651227.1"
                     /note="Involved in the cleavage of a C-terminal peptide of
                     11 residues from the precursor form of penicillin-binding
                     protein 3; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail-specific protease"
                     /protein_id="HDL5347276.1"
                     /translation="MKLTRLNKIFALALGLMIGNAFAVQPDIKESAIVMPKPNEQHSL
                     STKRVTARLTQSHYHKFQLNDEFAGKIFNRYVDWLDPSHNTFLQADIDEMRSKYATTL
                     DDDLYAGKLDAAFAMYELMSKRRYERYSYALSLLDKEPDLKGNDEIEIDREKAAFPKS
                     VEEANTL"
BASE COUNT          399 a          184 c          252 g          315 t
ORIGIN      
        1 ttagattttt attaaatggg acttattatg tcagagcaaa ttgagcaaaa agtagaacaa
       61 actcagaata aaaccaatcc aagtaccaag gaagtgattg cttatttagc ggagaaattt
      121 ccgctttgtt tcacgcttga aggtgaagta aaaccattga aagttgggtt gtttcaggaa
      181 ttagctgaag cattaaaaga tgatgagaaa atcagtaaaa cgttattgcg ccaagtattg
      241 cggaattaca ctaattcttg gcgttacttg gctgccagcc aagctaatgt tgctcgtgtc
      301 ggcttacagg gtgaagaagt tggcgtggta acagaagacg aagctactca cgccgcagaa
      361 gctttggctg ctgcgaaagc ggtggttgca gaacgtaaag cacaagagcg taaagcacaa
      421 cgtaaagagt acttcaaaca gaaagcacgt gaagaaaatg cgaaaaagcg tgcagaattt
      481 aaggcgaaaa aagcaataga taatttacct aaagcttcag aagaaaaatt agctgcatta
      541 gcagataaat tcggtcgtaa atagttttca agtcgctttg tgcttacaag cggtcggatt
      601 ttagtaaaat tttgcaaaaa aatgaccgct tgattatttg gaaaagaaga tgaaattaac
      661 aagattaaat aaaatttttg ccctagccct aggtttaatg ataggaaacg cttttgccgt
      721 tcagccggac attaaagaaa gtgcgattgt gatgccaaaa cctaatgagc agcatagctt
      781 atcaaccaag cgtgtaacag cccgattaac tcaatcgcat tatcataaat ttcaactgaa
      841 tgatgagttt gcagggaaga tttttaaccg ctatgtcgat tggcttgatc cttctcataa
      901 tacttttctt caagccgata ttgatgaaat gcgtagtaaa tatgctacaa cattagatga
      961 tgatttatat gctggtaaat tagatgcagc atttgcaatg tatgagttaa tgtctaaacg
     1021 tcgttatgag cgttatagct atgccttatc tttactagat aaagagcctg acttaaaagg
     1081 gaatgatgaa attgagattg accgtgagaa ggccgcattt ccaaaaagtg tagaagaggc
     1141 aaatacactt
//
LOCUS       DANLUQ010000255         1095 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.257, whole genome shotgun sequence.
ACCESSION   DANLUQ010000255 DANLUQ010000000
VERSION     DANLUQ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1095
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.257"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..>1095
                     /locus_tag="PXB27_002548"
     CDS             <1..>1095
                     /locus_tag="PXB27_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347277.1"
                     /translation="NHPLNIKYLHNKLYSFSLADRDEIWTAFIALESDQCPAIERLLT
                     WCQKSSGQENIEPEALLLGAIAVSWLFTSTDKRSRNGYINALAHLLWNRLEIALALFK
                     EFIEVNDPYVMEGILSAIHGAVAYSENSSGLKALALEIDNQIFNQPETEEIYPNVLVR
                     DYARHIIEYSLQKDEYSSDDIEIIRERITPPYRSSFPQNLPTNEEIDKKYHSDGQRII
                     IRSMTTEYGRGMCGYGDFGRYTFEAKFRNWESYKFGNLINVDLLSNYACQMIFEQFGY
                     DDKKHLEFDKETRSRYWRRDDTNKIERIGKKYQWLALYEILARVMDNFPTFSNRYADE
                     IGLEYITNLDNFYLPKTQIKIVPPTKTDVF"
BASE COUNT          371 a          164 c          201 g          359 t
ORIGIN      
        1 ttaaccaccc attaaatata aaatatttac ataacaaact ttattctttt tctttggcag
       61 atagagatga gatttggaca gcttttattg ctttggagtc tgatcaatgc ccagcaattg
      121 aacgattact aacttggtgt cagaaatcaa gtggtcaaga aaatattgaa cccgaagcac
      181 tattattggg tgcaattgct gttagttggc tatttacttc taccgataaa cgaagtagaa
      241 atggctatat taatgcactg gctcatttat tatggaatag attggaaatt gcattggcgt
      301 tattcaagga atttatagag gttaatgacc cttatgttat ggaaggtatt ttatctgcca
      361 ttcacggagc tgttgcttat tctgaaaatt catctggctt aaaagcactt gcattagaga
      421 tagataatca aatatttaat caacctgaaa cagaagaaat ttatcccaat gttttggttc
      481 gtgattatgc tcgccatatt attgaatact ctttgcaaaa agatgaatat tcatcagatg
      541 atattgagat aatcagagaa agaattacgc caccttatag aagttcattt ccccaaaact
      601 taccaaccaa tgaagaaatt gataaaaaat atcattctga tggacaaaga atcattatcc
      661 gttcaatgac aactgaatat ggtcgtggta tgtgtggata tggtgatttt ggcagatata
      721 cttttgaagc caaattccga aattgggaga gttataaatt cggcaatctc atcaatgttg
      781 atttattaag taattatgct tgccaaatga tttttgagca atttggctat gatgacaaga
      841 agcaccttga gtttgataaa gaaactcgtt caagatattg gcgaagagat gatactaata
      901 aaattgagcg aattggcaag aaatatcaat ggttggcttt gtatgaaatc ttggcaaggg
      961 tgatggataa ttttcctact ttttctaatc gctatgccga tgaaataggg cttgaatata
     1021 ttactaattt ggacaacttt tatctaccca aaacccaaat taaaattgta ccgccaacta
     1081 aaacagatgt atttt
//
LOCUS       DANLUQ010000256         1058 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.258, whole genome shotgun sequence.
ACCESSION   DANLUQ010000256 DANLUQ010000000
VERSION     DANLUQ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.258"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..942
                     /locus_tag="PXB27_002549"
     CDS             <1..942
                     /locus_tag="PXB27_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDL5347278.1"
                     /translation="DDLEKTVENFNNFKNSIDYDSDAWIFNKEEYRTYMNDNVIPFME
                     KVEEFTETLKTLSRDDLSAEERTTKTIDSVKLRIYLNEQARNRKNYINGFVPTDWIPE
                     ITDQMTKSKNLWLKYEEEAKTTLKPYQEETISRVIQDEINKKMAEIGTTTLEIGGKEH
                     ELEDLENQINALALTDEEKLAEDVENDLISKLDEAKAALAKNQQTLKEKRDELLGLNN
                     KLDSSPLVKKGKNALAEGTNAFASGENAIAFGTDSQATGNNAIALGANSKANAESAIA
                     IGKGAQALKEKALALGENAIANRASAIAIGDNHSSKL"
BASE COUNT          415 a          178 c          194 g          271 t
ORIGIN      
        1 gatgatttag aaaaaactgt agaaaatttc aataacttca aaaattcaat tgattatgat
       61 agcgatgcgt ggatctttaa taaagaagaa tatcgcactt atatgaatga taatgtaatt
      121 ccttttatgg agaaggtaga ggaatttact gaaacattaa aaactttatc tagagatgac
      181 ttaagtgcag aggaacgtac aaccaaaaca attgactccg ttaaattaag aatatatctc
      241 aatgaacaag cacgaaatcg taaaaattat attaatggat ttgtacctac cgattggatt
      301 cctgaaatta cagatcaaat gacaaaatcc aaaaatctat ggttaaaata tgaagaggaa
      361 gccaaaacaa cattaaaacc ataccaagaa gaaacaatat ctagggttat acaagatgaa
      421 attaataaaa aaatggctga aataggtact acaacactag agattggtgg taaagaacac
      481 gagctcgagg atcttgaaaa ccaaattaat gctttagctt taactgatga agaaaaactt
      541 gcggaagatg tggaaaatga tttaatctca aagcttgatg aggctaaagc agccttagca
      601 aaaaaccagc aaacgctcaa agaaaagcgt gatgaattac tagggctaaa taacaaatta
      661 gatagctcac cacttgttaa aaaaggtaaa aatgcacttg cagaaggcac gaatgccttt
      721 gcttctggtg aaaatgccat cgcattcggc acggattcac aagctacagg taataacgct
      781 atcgcactag gtgcaaattc aaaagcaaac gcagaaagtg cgattgcaat tggtaaaggt
      841 gcacaagcat taaaagaaaa agcccttgct ttaggcgaaa atgccattgc caacagagca
      901 tctgctattg cgattggtga taaccattca tctaaattgt agaagatcaa acctaatctg
      961 acagtccccc gttttaaatt accgtgtctg tcagattaat ttgagcttaa attcttttcc
     1021 acccaaatcc gttttccatc aagtaaggtt gccatcgg
//
LOCUS       DANLUQ010000257         1049 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.259, whole genome shotgun sequence.
ACCESSION   DANLUQ010000257 DANLUQ010000000
VERSION     DANLUQ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.259"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..497)
                     /locus_tag="PXB27_002550"
     CDS             complement(<1..497)
                     /locus_tag="PXB27_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5347279.1"
                     /translation="MELEQQIIDYALQHEPHEMCGFVVFDGNKNRFIPCENQAEDKAN
                     YFEISDLDYIKAEEKGELVAVVHSHPEPNGKPILSVLDRKMQVQTGLDWWLVHNRQIH
                     KFRNVPHLIGREFKHGTMDCYTLYRDAYMLAGYEMDEFERQDDWWHTGQNLYLDNIQG
                     QGFERV"
     gene            complement(501..>1049)
                     /locus_tag="PXB27_002551"
     CDS             complement(501..>1049)
                     /locus_tag="PXB27_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HDL5347280.1"
                     /translation="VWQGRTYDPYPIEATGFERNSSGPSNRPTLTLSNLFGLVTGIAN
                     QFDECIGAIVRRHQVYAQYLDAVNFAGGNAKADPNQEIISHFVIEQLSSLTRETATFT
                     LALPIETDNAKIPSRIIMADTCTWIYRSAECGYTGKQYFDEKDKATSDPKADKCSHCL
                     NGCELRGNQRNFGGFVSVNKLG"
BASE COUNT          263 a          247 c          230 g          309 t
ORIGIN      
        1 actcgctcaa aaccttgccc ttggatatta tccaaataaa gattttgccc ggtatgccac
       61 cagtcatctt gtcgctcgaa ctcgtccatc tcgtaaccgg caagcatata ggcatcacgg
      121 tagagcgtgt aacaatccat tgtgccgtgt ttaaactcac gcccgattaa atgtggtaca
      181 ttgcggaatt tatggatttg ccgattatgc actagccacc aatctaaacc ggtctgtacc
      241 tgcattttgc gatcaagcac agataatata ggcttgccgt ttggctcggg atgtgaatgt
      301 accaccgcta ccagttcgcc tttttcttct gctttgatgt aatcaagatc ggatatttca
      361 aagtaattgg ctttgtcttc cgcttggttt tcgcaaggga taaatcgatt tttattgccg
      421 tcaaaaacaa caaagccgca catttcgtgc ggttcgtgtt ggagggcgta gtcgattatt
      481 tgttgttcca attccattca ttaccccaat ttattcaccg atacaaatcc accaaaatta
      541 cgctgattgc ctcgcaattc acaaccgttc aggcaatggc tgcatttatc ggcttttggg
      601 tctgatgttg ccttgtcttt ttcatcaaaa tactgcttgc cggtgtagcc acattccgcc
      661 gagcggtaaa tccaagtgca ggtgtccgcc ataataattc tgctggggat tttggcatta
      721 tcggtttcaa tcggcaacgc aagcgtaaaa gtagcagtct ctcgtgtgag gcttgaaagt
      781 tgctcaatca caaaatggct gatgatttcc tgattaggat cggctttggc attacctcca
      841 gcaaagttta ctgcatctaa atattgggcg taaacctgat gacggcgaac aatcgcaccg
      901 atacattcgt caaactgatt agcaataccc gtaactaagc caaacagatt tgatagcgta
      961 agagtagggc gattgcttgg gccgctgctg ttacgctcaa atcctgttgc ctcgatgggg
     1021 taagggtcgt aagttcgacc ttgccagac
//
LOCUS       DANLUQ010000258         1006 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.260, whole genome shotgun sequence.
ACCESSION   DANLUQ010000258 DANLUQ010000000
VERSION     DANLUQ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.260"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..>1006
                     /locus_tag="PXB27_002552"
     CDS             <1..>1006
                     /locus_tag="PXB27_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HDL5347281.1"
                     /translation="FTAEWTSERAYLLVGVNGTYETTDWLAFHSVKLEKGNVATDWTP
                     APEDVDSAVSAVSAKIDTIQETLANADSALSSRIDAVTASVGSNLAKITQVSNAVAGV
                     DGKLSATHTIKTETIAGGRKAIAGIALGAAADNRNAESSVIVMADKFEVVKNAQDGQP
                     VRLFGVAQNKVAINGDLIATGTIKGSHIDADSVRAGILTAGAIRTEHLAAGQISADKL
                     AIGLGGNLLVNPIFATPDLSIAPFGWNYWRGEVGDRLNIDTRNIFNRNEQDNYGLKNG
                     GLPNELVFSMRYSTTKETSDTGRIGWLSQDINVVPNKKYIASVWLACHRGQAKLVIEN
                     "
BASE COUNT          294 a          192 c          255 g          265 t
ORIGIN      
        1 cctttactgc cgaatggaca agtgagcgag cctatctgct tgttggtgta aatggtactt
       61 atgagaccac agattggtta gcgtttcaca gcgtcaaact tgaaaaaggc aacgtagcca
      121 cagactggac gccagcacct gaagatgtgg acagtgcggt tagtgcagtt tcggctaaaa
      181 ttgatactat tcaggagact ttagcgaatg ccgactctgc attaagctct cgtattgatg
      241 ccgttactgc ttctgttggc tcaaacttgg caaaaattac tcaagtgagt aatgctgtag
      301 ccggtgtgga cggcaaatta tctgctaccc ataccatcaa aaccgaaacc attgccggtg
      361 gacgtaaagc gattgccggt atcgcacttg gtgccgcggc agacaatcgc aatgcagaat
      421 cctctgtcat tgtaatggca gacaaatttg aggtagtaaa aaatgctcaa gacggccaac
      481 cggtacgatt gtttggtgtg gctcaaaata aagttgcgat taatggggat ttaattgcaa
      541 caggtacaat taaaggtagc cacattgatg cagactcagt aagagccgga atattgacgg
      601 cgggagctat ccgtacggaa cacctggcag cagggcaaat atcggcggat aagttagcga
      661 ttgggctagg tgggaatttg ctggttaatc ctatttttgc aacacccgat ttgtcaatag
      721 ctccgtttgg ctggaactac tggcgaggag aagttggtga tcggctaaat attgacaccc
      781 gtaatatttt caataggaat gaacaagata attacggact aaaaaatgga ggactaccaa
      841 atgagttagt attttcgatg cggtatagca ctacaaagga aacctctgat actgggcgaa
      901 ttgggtggtt atctcaggat attaatgttg tgcctaataa aaaatatatt gcctccgtat
      961 ggttagcttg tcatcgtggg caagctaaat tagtcattga gaatat
//
LOCUS       DANLUQ010000259          968 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.261, whole genome shotgun sequence.
ACCESSION   DANLUQ010000259 DANLUQ010000000
VERSION     DANLUQ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..968
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.261"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            1..>968
                     /locus_tag="PXB27_002553"
     CDS             1..>968
                     /locus_tag="PXB27_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015586968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL5347282.1"
                     /translation="MHFYIISDDLTYKSISLEGINSKFSWLTLNNKSDSILFVESTWQ
                     GYKNRWKYKIASYPDHPKRTNQKLLRLVEKAKDKGIPTVFWNKEDSIHFDRFIDSAKH
                     FDHIFTVDQNAIARYREVVPDTTTVNVGMFPVQPRIHNFQGFNFKHLAANFVGSYSHH
                     IHNKRRERQDMLFGAALKAGLPVTIFDRNSDRKSENYRYPKNSFNLNVQPAVSYTETA
                     NIYRDYLVSLNINTVEDSPTMFSRRVVEILACGGILVSTPSMAIDNIFKEYCHIVQSE
                     DEAIELFQRLKRGASRDDLNKAIAGSEFVLSNFTWEHFLSQIQKVV"
BASE COUNT          320 a          158 c          172 g          318 t
ORIGIN      
        1 atgcattttt atattattag tgatgattta acttataaat ctatttcact agaaggcatc
       61 aactctaaat tctcctggtt gacgttgaat aacaaatcag attcaatatt atttgtagaa
      121 tctacttggc aaggctacaa aaaccgttgg aaatacaaaa tagcatctta tcctgaccac
      181 cccaaacgaa ccaatcaaaa attgttgcgt ttggtggaaa aagcaaaaga taaaggtatt
      241 ccaacggtat tttggaacaa ggaagattcc atacatttcg accgatttat tgattcagcg
      301 aagcattttg atcatatatt tactgtagat caaaatgcta ttgctcgtta tagagaggtt
      361 gtacctgata caaccactgt caatgtaggt atgtttcctg ttcagccaag gattcataat
      421 tttcaaggat ttaattttaa gcaccttgcc gcaaattttg tagggagtta cagtcatcat
      481 atccacaata agcgtagaga aagacaggat atgttgtttg gtgcagcact taaagcaggt
      541 cttccggtaa ccatctttga tagaaactct gatagaaaat cggaaaatta tcgttaccca
      601 aaaaatagct ttaatttaaa tgttcaacct gctgttagtt atactgaaac tgctaatatt
      661 tatcgtgatt atcttgtttc tttgaatatt aatactgtcg aagattcccc tacgatgttt
      721 tctcgcagag tcgttgaaat attagcttgt ggaggaatat tagtctctac accaagtatg
      781 gctatcgaca atatatttaa agaatattgt catatcgtac aaagtgaaga tgaggctata
      841 gaattattcc aacgtttaaa acgaggtgct tctcgtgatg atttgaataa agcgattgca
      901 ggctctgaat ttgtattgag taactttact tgggaacatt tcttatccca aatacagaaa
      961 gttgtata
//
LOCUS       DANLUQ010000260          963 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.262, whole genome shotgun sequence.
ACCESSION   DANLUQ010000260 DANLUQ010000000
VERSION     DANLUQ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..963
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.262"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(33..>963)
                     /locus_tag="PXB27_002554"
     CDS             complement(33..>963)
                     /locus_tag="PXB27_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347283.1"
                     /translation="SLKHIKNLESNSYLDEEGRDTVNKYKAFFSIFEAEIRDATCICD
                     NKIVTINGEVCCDIKEVARKIVIEFLNEVDIGNKVKFYYPDERHELFVELLIVLATLR
                     IYRNRNIFFFEKGFFNFFPEFITILGTGAFNGFLDFPSLVGKVFPEIIDTINEGLDLD
                     IAIENFEVLSQEEMTECMGLYEQALIKKAEEGEIGLLNVDEINSFYEDNSELILLAKQ
                     PMVEEIIPKPDSKASSAILKFFDSNSNILIAKLKESGLQLSAVALQNDQNIARVANVV
                     YNLLPGMVRIFVSYDTVENFLLNNRQWLINKLV"
BASE COUNT          316 a          192 c          115 g          340 t
ORIGIN      
        1 atattgttta tccatagttc ttcccctcca aattaaacta atttattaat caaccattgg
       61 cggttattta ataagaaatt ttctacggta tcgtaactta cgaaaattct taccatgccc
      121 ggcaataagt tataaacgac atttgctact cgtgcgatat tttggtcgtt ttgtaatgcc
      181 acagcactta attgcaaacc actttctttt aatttagcaa ttaagatatt tgagtttgaa
      241 tcaaagaatt ttagaattgc agaggaggct ttgctatctg gtttagggat tatctcttca
      301 accataggtt gtttagcaag taagattagc tcactattat cttcataaaa gctatttatt
      361 tcatcaacat ttaataaacc aatttcgcct tcttcagctt tcttaatgag agcctgctca
      421 tataaaccca tacattcagt catttcttcc tgactcaaca cttcaaagtt ctcaatcgca
      481 atatctaaat ctaaaccctc atttatcgta tcaataattt ctggaaaaac ctttcctacc
      541 aaactaggga agtctaaaaa tccattaaac gcaccagtcc cgagaatagt gataaattcg
      601 ggaaaaaagt taaaaaaccc cttttcaaag aagaatatat ttctattacg atatattctt
      661 aatgttgcta gaacaatgag cagctctaca aaaagctcat gtctttcatc tgggtaataa
      721 aacttaactt tattaccaat atccacttca tttagaaatt ctataacaat tttccttgct
      781 acctccttaa tatcacagca cacctcacca ttaattgtta caattttatt gtcacaaata
      841 catgttgcat ctcgaatctc tgcttcaaaa atagagaaaa atgccttgta tttatttacc
      901 gtatctcttc cttcttcatc taaataactg ttagattcta gatttttgat atgtttcaaa
      961 gat
//
LOCUS       DANLUQ010000261          893 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.263, whole genome shotgun sequence.
ACCESSION   DANLUQ010000261 DANLUQ010000000
VERSION     DANLUQ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.263"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            12..638
                     /locus_tag="PXB27_002555"
     CDS             12..638
                     /locus_tag="PXB27_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HDL5347284.1"
                     /translation="MTKIKFYGNLKKFGSEFSLEVKDTAEAIRALCTQISGLREALRD
                     GVYKVRIGKQYLDPSALEKGLFYCLKKGQTIHFTPVIKGAKSGGVFNFVLGAALIGAA
                     FFTGGASIAAWGAGAKMMGMLGASMLLGGVSQMLTKMPKAPTMGNETEKESSTAFSNL
                     NNLVTQGKPVPLAYGLIRTGSLVISQGVETITIKENQPASNKKTGFRK"
     gene            687..>893
                     /locus_tag="PXB27_002556"
     CDS             687..>893
                     /locus_tag="PXB27_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5347285.1"
                     /translation="MNLTKAESNLNAKITEEKKARVDADKVNADKLTDITSRVANAES
                     TITNFQSTKANKSEVASLAQSSLQA"
BASE COUNT          297 a          167 c          206 g          223 t
ORIGIN      
        1 aatggtgagt catgactaaa attaaatttt acggcaattt aaaaaaattt ggtagtgaat
       61 ttagcttaga agttaaagat actgccgaag ctatccgagc cttatgtaca cagattagcg
      121 gattgcgtga ggcattacga gacggtgttt ataaagtccg tatcggcaaa cagtatttag
      181 acccgtcagc ccttgaaaaa gggctttttt attgtctgaa gaaagggcaa accattcatt
      241 ttacccctgt tatcaaagga gcgaaaagcg gtggcgtgtt taattttgtt ttaggtgcag
      301 ccttgatcgg agccgcattt tttactggcg gtgcttctat tgcggcttgg ggagctggag
      361 caaaaatgat gggtatgtta ggggcttcga tgttactagg aggagtttcc caaatgctca
      421 ccaaaatgcc aaaagccccc acaatgggta atgaaacaga aaaagaaagc tcaacggctt
      481 tttcaaattt aaataacttg gtgactcaag gcaaacctgt gccgttggct tatggcttaa
      541 ttcggaccgg ctcgcttgtg atatcgcagg gagttgagac gatcaccatt aaagaaaacc
      601 aaccggcaag caacaagaaa acaggcttta gaaaataagg gataattacc gattcagcac
      661 aagcacagca acttaatcag ttatctgtga acttgaccaa agcagagagc aatttaaatg
      721 ccaaaatcac ggaagaaaaa aaagctcgtg ttgatgcaga taaagtgaat gcagataaac
      781 tcacagacat aacaagccgt gttgcaaatg cggaatcaac tattaccaat ttccaatcaa
      841 ccaaagcgaa taaaagtgag gttgcgagcc ttgcacaaag ttcgttgcag gca
//
LOCUS       DANLUQ010000262          873 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.264, whole genome shotgun sequence.
ACCESSION   DANLUQ010000262 DANLUQ010000000
VERSION     DANLUQ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.264"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            26..>873
                     /gene="msrA"
                     /locus_tag="PXB27_002557"
     CDS             26..>873
                     /gene="msrA"
                     /locus_tag="PXB27_002557"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005546455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL5347286.1"
                     /translation="MKYNQLLLCLVSAIFTTSSIANITTPNEMRSENLQDIYLAGGCF
                     WGIEAYMEKIYGVKEATSGYANGKTEQTAYRIIGQTDHAETVHVTYDANKISLEKLLQ
                     YYFKVIDPTSKNKQGNDIGRQYRTGIYYQNEQDKQIILKEIQAQQAKYKQKIQVQVEP
                     LKHYILAEEYHQDYLKKNPNGYCHIDLNQANSIIIDPKDYPKPSDATLKMKLTPLQYS
                     VTQRKNTERSFSNEYWDNTAPGIYVDITTGEPLFSSLDKYDSGCGWPSFTKPIINEVV
                     NYTEDN"
BASE COUNT          329 a          161 c          152 g          231 t
ORIGIN      
        1 tacataagtt ataaaggaga actggatgaa atacaatcaa ctgttactat gtttggtgtc
       61 tgctatcttt accacctcct caatagcaaa catcacaacc cctaacgaga tgcgatctga
      121 aaacttacag gatatttact tggcaggagg atgtttctgg ggcattgaag cctatatgga
      181 aaaaatatat ggcgtaaaag aggctacatc gggctatgcc aatggcaaaa ctgagcaaac
      241 tgcttaccga attatcgggc aaaccgatca tgcagaaaca gttcacgtga cctatgatgc
      301 taataaaatt tctttagaaa agctgttgca atattacttt aaagtaattg atccaaccag
      361 taaaaataaa caaggtaatg atattggccg ccaatatcgg acaggtattt attatcagaa
      421 tgagcaagat aaacaaatta ttttaaaaga aatacaagct caacaagcaa aatacaaaca
      481 aaaaatacaa gtgcaagtag aaccattaaa acattatatt ttggcagaag agtatcatca
      541 ggattaccta aagaaaaatc caaatggcta ctgccatatt gatttaaacc aagccaacag
      601 cattattatc gatccgaaag attatcctaa acctagtgat gcaacattaa aaatgaaatt
      661 aacgcctttg caatatagcg ttacccaacg taaaaatact gagcgttcat tcagcaatga
      721 atattgggat aataccgcac cgggcattta tgttgatata actacaggag agcctctttt
      781 ttcatctctc gataaatatg actccggctg tggctggcca agttttacca aaccaattat
      841 caatgaggtt gttaactata ccgaagataa tag
//
LOCUS       DANLUQ010000263          855 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.265, whole genome shotgun sequence.
ACCESSION   DANLUQ010000263 DANLUQ010000000
VERSION     DANLUQ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..855
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.265"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            123..305
                     /locus_tag="PXB27_002558"
     CDS             123..305
                     /locus_tag="PXB27_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDL5347287.1"
                     /translation="MKYSEFLRYLLAQGCEIENHRRGSHRKVTLNGKQSVFPYHGSKE
                     IGTGLVNKIKKDLDLK"
     gene            344..763
                     /locus_tag="PXB27_002559"
     CDS             344..763
                     /locus_tag="PXB27_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012621799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDL5347288.1"
                     /translation="MLRYPVEITPDDNGTYLVTCPDIPEMASVGEDLEEALLEAQDGL
                     ATALEFYFDDRREIPMPSPIKEGQHTVNLTVLQSMKVFLLNEMIKQGVRKAEMARRLD
                     VHLPQIDRLLDFNHSTKVEFVEKAYGKLNQHFTILPH"
BASE COUNT          291 a          165 c          159 g          240 t
ORIGIN      
        1 ccttgaataa accgccctcg tggcggtttt cttttctgct acaaaaaaaa cacaacaaaa
       61 atgttgtaat accaacaaaa atgttgtatt atttccttgc tgttttgata aggaggtatc
      121 ttatgaaata cagcgagttc ctgaggtact tacttgctca aggttgcgaa attgaaaacc
      181 ataggcgagg cagtcatcga aaggtaacac taaacggaaa acaatcggtt tttccttatc
      241 acgggagcaa agagattggt acaggtttag taaataagat taagaaagac ttagatctta
      301 aataacaaag tccctcgaaa gaggggcttt tctaggagtt tatatgttac gctacccagt
      361 tgaaattacc cctgatgata acggcacata tcttgtgact tgccctgata ttcctgaaat
      421 ggcaagcgta ggtgaggact tagaagaagc cctattagaa gcacaagatg gcttagccac
      481 agcccttgaa ttctattttg atgatagacg agaaatacca atgccaagcc caattaaaga
      541 gggacaacat acggttaatc ttaccgtgct gcaatcaatg aaagtatttc tcttaaatga
      601 aatgattaaa caaggtgtac gcaaagcaga aatggcaaga cgattagatg tacacttacc
      661 acagatagat cgcctattag attttaatca ttccacaaaa gtagaatttg ttgagaaagc
      721 ctatggtaag ctaaatcaac actttactat tctccctcac taataaaata accgcctacg
      781 ggcggttttc tttttcctaa aatctgcaaa attctcttca aaaacgaccg cttgcaactc
      841 aatctgttta tttat
//
LOCUS       DANLUQ010000264          833 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.266, whole genome shotgun sequence.
ACCESSION   DANLUQ010000264 DANLUQ010000000
VERSION     DANLUQ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.266"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            17..688
                     /locus_tag="PXB27_002560"
     CDS             17..688
                     /locus_tag="PXB27_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HDL5347289.1"
                     /translation="MKKSTLAIILGGALLTGTAVAHQAGDVIFRAGAVHVKANSSSDA
                     KSKAATGIDVDLEVKNNTQLGLTATYMLADNVGIELLGATPFSHKINAKAKVLESGAE
                     VDLDRVVILKQLPPSLYAQYYFFELTAKVKPYVGAGLNYTRFYHAKSVSPAVTDLSVK
                     KHSFGPVANLGVDVKLTDNIYFNAAAWYTRIKTTAKFKAAGADHEVKVKLDPVVLFAG
                     FGVKF"
BASE COUNT          268 a          138 c          169 g          258 t
ORIGIN      
        1 ttttgaggat atgaaaatga aaaaatcaac tttagcaatt attttaggtg gggcattatt
       61 aactggaact gcggtggcac atcaagcagg tgatgtgata ttccgtgccg gtgcggtaca
      121 tgttaaggca aattcaagtt cagatgcaaa atcaaaagca gcgacaggta ttgatgtaga
      181 tttagaagtg aaaaacaata ctcagttagg cttaactgca acttatatgt tagccgataa
      241 tgtgggtatt gagttattag gtgcaacccc tttctctcat aaaattaatg ccaaagccaa
      301 agttttagaa tcaggtgcag aagtagattt ggatagagtt gttatcttaa aacaattacc
      361 accgagcctt tatgcccaat actatttctt tgagctaact gcaaaagtaa aaccttacgt
      421 tggtgcaggt ttaaattaca ctcgcttcta ccacgctaaa tcagttagcc ctgctgttac
      481 tgacttaagt gtaaaaaaac attctttcgg accggtagca aacctaggtg ttgatgtaaa
      541 attaaccgat aacatctact tcaacgcggc agcttggtac acacgcatta aaacaaccgc
      601 aaaatttaaa gcagcaggtg cagatcacga agtaaaagta aaacttgatc cggttgtctt
      661 atttgccggt ttcggggtga agttctaatt atttgttggg aacgagttgg gctaatctta
      721 gtgattagtc cttttttgtt aaagttgttt gaatatagcc ctttccactt ttaattaaat
      781 aattgaagag ataataaaat ctcactatgc aaatatttat tggtgtaatt ggt
//
LOCUS       DANLUQ010000265          823 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.267, whole genome shotgun sequence.
ACCESSION   DANLUQ010000265 DANLUQ010000000
VERSION     DANLUQ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.267"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            146..790
                     /locus_tag="PXB27_002561"
     CDS             146..790
                     /locus_tag="PXB27_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL5347290.1"
                     /translation="MFGHIWQYAREWGYTTLASPNTGMKRFPIRKREIYVENNIFEMV
                     YEVADQDIKDLMDLAYLTGQRPVDLVNIQRDHIFDGYLHIVQQKTQAKLRIEIVGKLA
                     DIIQSRLEKTTRNFLFTTSKGEKMTSVKLGRRFTYIREQAAKKHPEYANEIKEFQFRD
                     LRAKSGTDKALAQGEEAARQQLGHTSVQMTKVYIRKAPIVTPIQESVSSQKHKP"
BASE COUNT          326 a          161 c          141 g          195 t
ORIGIN      
        1 ttactaaatg tttaatattt actaaactac acccatcaaa acaaacaacg ccacagacaa
       61 aggggaaacc aaaatgaacg cacaaacaac gctaaacaac cacaaagact acattcacta
      121 agaattttta tcaaagccct tttcaatgtt cggtcatatt tggcaatacg caagagagtg
      181 gggttacaca acgttagcta gtcctaatac aggaatgaaa cgctttccaa ttagaaaacg
      241 tgaaatttac gttgagaaca atatttttga aatggtgtat gaagtagcag atcaggatat
      301 taaagattta atggatctcg cttaccttac aggacaaaga cctgtagatt tagttaatat
      361 ccaacgcgat catatatttg atggatatct ccatattgtg caacaaaaaa cacaagccaa
      421 actgcggatt gagatagttg gcaaacttgc tgatataatt caaagtaggt tagaaaaaac
      481 cacaaggaat ttcttattta caacgtcaaa aggagaaaaa atgacctctg taaaattagg
      541 aagacgcttt acttatatcc gagaacaagc agcaaaaaaa catcccgaat acgcaaatga
      601 aataaaggaa ttccaattta gagatctaag ggctaagtca gggacagaca aagcccttgc
      661 acaaggagaa gaagctgcac gccaacaatt aggtcatact agcgttcaaa tgacaaaagt
      721 ctatatccga aaagccccga tagtaacccc aattcaagaa agcgtttcct cccaaaaaca
      781 caaaccttaa aacctagttt cggaacatct gagaattttt cgg
//
LOCUS       DANLUQ010000266          763 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.268, whole genome shotgun sequence.
ACCESSION   DANLUQ010000266 DANLUQ010000000
VERSION     DANLUQ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.268"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..>763)
                     /locus_tag="PXB27_002562"
     CDS             complement(<1..>763)
                     /locus_tag="PXB27_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347291.1"
                     /translation="SHCTLLNACSKALGFSGGFAGFQEDYLSNLTQFMEKHGLLYLVD
                     LTKQKYPVNDMCMTRSLALKKQDISERIFFSGKEIPKRIFTGFNFRYDMHLANGFRIG
                     NSLKTPYSDIGICEDINEALKNPSIKIDCRDGTIRSMADVVIGRYLDKVLFYYFNLIG
                     DQLVQPRLDKYEIQCYYPQGYQGDINNDLALHKKFLDFFVSRIEFLDKGWVDVIPYND
                     NLVFLKGENGEYDFVYKNQRSELFEHQIYSPFLKRS"
BASE COUNT          258 a          135 c           99 g          271 t
ORIGIN      
        1 gagcgtttta aaaatgggga atatatttgg tgttcaaaaa gttcacttct ctgattttta
       61 tatacaaaat catattcacc attttctcct ttaagaaaga ccaaattatc attatagggt
      121 attacatcta cccatccttt atctaaaaat tcaattcttg atacaaaaaa atctaaaaat
      181 ttcttatgta gagctaagtc attgttaata tccccttgat aaccttgagg gtaataacat
      241 tgtatttcat atttatccaa tctaggctgg actaactgat cacctatcaa gttaaaataa
      301 taaaacaata ctttatcaag atatctacct atgactacat ctgccatact tctgattgtg
      361 ccatcacggc aatcaatttt tattgatgga ttttttaatg cttcatttat gtcttcacat
      421 atacctatgt cagaatatgg ggtttttaag gaatttccga tacgaaaacc attagctaaa
      481 tgcatatcat atcggaaatt aaatccagta aatattctct tagggatttc ctttccgcta
      541 aagaaaattc tttcagatat atcttgcttt ttcagagcta agctccttgt catgcacata
      601 tcatttactg gatatttttg ttttgttaaa tctacaagat ataagagccc atgtttttcc
      661 atgaattgcg tcaaattaga taaataatct tcttgaaacc ctgcaaagcc tccagaaaat
      721 cctaaagctt ttgagcaagc atttaacaaa gtacagtgag aaa
//
LOCUS       DANLUQ010000267          749 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.269, whole genome shotgun sequence.
ACCESSION   DANLUQ010000267 DANLUQ010000000
VERSION     DANLUQ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.269"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..704
                     /locus_tag="PXB27_002563"
     CDS             <1..704
                     /locus_tag="PXB27_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HDL5347292.1"
                     /translation="FKAKIALQGAQLLSWQPYNAEQDVLWLSEIEPFRIGSAIRGGIP
                     ICYPWFGSIKEPAHGTARIQEWRLVEHTCSDENVGLVFALENKAKMEMMLGETCELYF
                     THLDPEPAQLALHSYFNIGDIEKVEVQGLPTRCFDKLTDQEVEVPSPRKISENVDCIY
                     PAQAVNFIQDFANQRTIKVEHINATETVLWNPWHNTVSAMTETAYQNMVCVETARINT
                     LLQQNETLGVRVLVR"
BASE COUNT          236 a          125 c          175 g          213 t
ORIGIN      
        1 aattcaaagc taaaattgca ttacaaggag cacaattgct aagttggcag ccgtataatg
       61 cagagcaaga tgtgctgtgg ttaagtgaga tagaaccgtt tagaattggc tctgcgattc
      121 gtggcggtat accaatttgt tatccgtggt tcggttcaat aaaagagcct gcccacggca
      181 cagctcgcat tcaagaatgg cgtttagttg agcatacttg ttctgatgaa aatgtcggtt
      241 tggtatttgc cttagagaat aaagcgaaaa tggaaatgat gttaggcgaa acttgtgagt
      301 tatattttac ccatttagat ccagaaccag cacaactggc attgcactct tattttaata
      361 ttggtgatat tgaaaaagtg gaagtgcaag gcttgccaac tcgctgtttt gataagctaa
      421 ccgatcaaga agttgaagtg ccatcacctc gcaagataag tgaaaatgtg gattgtattt
      481 atccggcaca agcggtgaat tttattcaag attttgcaaa tcagcgcacc attaaagttg
      541 agcatattaa tgcgaccgaa accgtacttt ggaatccttg gcataatacc gttagtgcga
      601 tgacagaaac ggcgtatcaa aatatggtat gtgtggaaac cgctcggata aatactctac
      661 ttcaacaaaa tgaaacgcta ggagtgaggg ttttagtgcg atagtgttta atgagagcaa
      721 gcagctgaat tagtaatatt gtttacaaa
//
LOCUS       DANLUQ010000268          749 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.270, whole genome shotgun sequence.
ACCESSION   DANLUQ010000268 DANLUQ010000000
VERSION     DANLUQ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.270"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..126
                     /locus_tag="PXB27_002564"
     CDS             <1..126
                     /locus_tag="PXB27_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMP-N-acetylneuraminate-beta-galactosamide-
                     alpha-2, 3-sialyltransferase"
                     /protein_id="HDL5347293.1"
                     /translation="ILNKSNHIEVVALRVNVDNPAYIESYELLEKLGVQIVDIRE"
     gene            128..>749
                     /locus_tag="PXB27_002565"
     CDS             128..>749
                     /locus_tag="PXB27_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HDL5347294.1"
                     /translation="MKKYLISLEKDVKRRELFFSQPNTEDFEVFSAINTMSSDWEALN
                     NHFDLALFEQRYGRKTTKGEVGCTLSHLAVYQKIAEDSTIAEDDYCLVCEDDALFADD
                     FQKHLDALLAQNLKVDIILVGQSKIPTFNDSELELNYPSTFGFLQKNIPNSQYKYAYS
                     YLNYFAGTVAYLIKKSTACQFLAQCAGKNPYWLADDFILFEKNFKFD"
BASE COUNT          254 a          105 c          143 g          247 t
ORIGIN      
        1 attttaaata aaagtaacca tattgaagtg gtagcattaa gagtgaatgt agataatcct
       61 gcttatattg agagctatga attgcttgaa aaattaggcg ttcagattgt tgatattaga
      121 gaataaaatg aagaaatatt taatttcatt agaaaaagat gtaaaaagac gagagctatt
      181 ttttagccag cctaatacgg aagattttga ggtgttcagt gccattaata caatgagtag
      241 tgattgggag gcattaaata accattttga tttggctctg tttgaacagc gatatgggcg
      301 taaaaccact aagggcgaag taggttgtac tttaagccat ttagccgttt accaaaaaat
      361 tgccgaagat agcactattg cggaagatga ttactgccta gtctgtgaag atgatgcctt
      421 atttgccgat gatttccaaa aacatcttga tgctttatta gcacaaaatt taaaagtcga
      481 tattatttta gttgggcagt cgaaaattcc aacttttaac gatagcgaat tagagcttaa
      541 ttacccatca acctttggct ttttgcaaaa aaatattccg aatagccaat ataaatatgc
      601 ttattcttac ctgaattatt ttgctggtac ggttgcatat ttaattaaaa aatcgaccgc
      661 ttgtcagttt ttagcacaat gtgccggaaa aaatccatac tggcttgccg atgattttat
      721 tttatttgaa aagaatttca aatttgatg
//
LOCUS       DANLUQ010000269          741 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.271, whole genome shotgun sequence.
ACCESSION   DANLUQ010000269 DANLUQ010000000
VERSION     DANLUQ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.271"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..696
                     /locus_tag="PXB27_002566"
     CDS             <1..696
                     /locus_tag="PXB27_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347295.1"
                     /translation="ALVLAEWTKEYHGKYDNFINDIKSDNWTSAAINFHLGMEKVPLN
                     DLIYKEIYNYWDKTSLKDESERIISASIEFSNYFANKYSENIEEGIKNAEKEFKKNYN
                     KSLRYNAKKAQQRVQKYVEKVNQKNPQALKDLDNISNLKKINENLKKAAKFTGIISSI
                     KDYYDLAEGFRNGIKSNDWEPFLKTSAQIGASALMSMLVASMITGPTTIGSIIISAAF
                     ISIGGYIISLASR"
BASE COUNT          294 a          102 c          127 g          218 t
ORIGIN      
        1 gctttagtac ttgccgaatg gactaaagaa tatcatggca aatatgataa ctttattaat
       61 gacatcaaat cagataattg gacaagtgca gcaattaact ttcatttagg tatggagaaa
      121 gtaccattaa atgatcttat ttataaagaa atatataact actgggataa aacatcattg
      181 aaagatgaat cagagaggat tatatcagcc agtattgaat tcagtaacta ttttgctaat
      241 aagtactctg aaaacattga agaaggcatt aaaaatgcag aaaaagaatt taaaaagaat
      301 tataataaaa gtctaagata taatgctaaa aaagcacagc agagggtgca aaaatatgtc
      361 gaaaaagtca accaaaagaa tcctcaggca ctaaaagatc tcgataacat atccaactta
      421 aaaaaaatta atgagaattt aaaaaaagca gcaaaattta ctggtattat tagcagtatt
      481 aaggattatt acgatctagc agaagggttt agaaatggca taaagtctaa tgattgggaa
      541 ccttttttaa agacatctgc tcagataggt gcttcagctt taatgagtat gttagtagcc
      601 tcaatgataa caggcccaac gacaattggg agtattatta ttagtgctgc atttatttct
      661 atagggggat atattatttc actagcttcc cgttagatag tctttgttat actcaataac
      721 ctactatact acctataggc t
//
LOCUS       DANLUQ010000270          739 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.272, whole genome shotgun sequence.
ACCESSION   DANLUQ010000270 DANLUQ010000000
VERSION     DANLUQ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..739
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.272"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..>739
                     /locus_tag="PXB27_002567"
     CDS             <1..>739
                     /locus_tag="PXB27_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HDL5347296.1"
                     /translation="FSPLSAKDQIFFKNESFETGKSLNLDEVFDRNLSVETPNGFDVI
                     LEDKKTGNLSGVNQSNIKALQFFIYQSQQTEEPLQRRFENSEIYGPFVVKSENHTYNQ
                     YFIKAVDAQKEWINTILDSPFIMAVLLMFAGIPLLIWLSFKITKPVKELTISANAVAS
                     GNLETNPKLEAEWIYEIGEVGKSFNHMVISLKNLTQQQQRMISDISHELKTPLARLQL
                     AIAILRRKTCGLAEIDRIEAEVGKLDQL"
BASE COUNT          270 a          113 c          130 g          226 t
ORIGIN      
        1 ttttctcccc tttctgcaaa agaccaaatt ttctttaaaa atgaaagctt tgaaacaggg
       61 aaaagcttaa atttagatga agtttttgat agaaatttat cagttgaaac acctaatgga
      121 tttgatgtaa tcctagaaga taaaaaaact ggtaatttaa gtggcgtaaa ccaaagtaat
      181 attaaagcat tgcagttttt tatttatcaa tcacaacaga ctgaagagcc gttacaaaga
      241 cggtttgaaa atagtgaaat ctatggtcca tttgtcgtga aatccgaaaa ccatacttat
      301 aaccaatatt ttattaaagc ggtagatgct caaaaagagt ggattaatac gattttagac
      361 tcacctttta ttatggcagt attacttatg tttgccggaa ttcctttatt aatatggtta
      421 tcctttaaaa ttaccaaacc ggttaaagaa ttaactatct cggcaaatgc ggtagcctct
      481 ggtaatttgg aaactaatcc taaattagaa gcagaatgga tctatgaaat tggggaagtt
      541 ggaaaaagct ttaatcatat ggtgataagc ttaaaaaact taactcagca gcaacaaaga
      601 atgatttcgg atatatctca tgagttaaaa acacctttag ccagattaca actcgctatt
      661 gctatattac gccgtaaaac ctgtggtctg gcagaaatcg atcgcattga agcagaagta
      721 ggtaaattag accaattga
//
LOCUS       DANLUQ010000271          723 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.273, whole genome shotgun sequence.
ACCESSION   DANLUQ010000271 DANLUQ010000000
VERSION     DANLUQ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.273"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(84..716)
                     /locus_tag="PXB27_002568"
     CDS             complement(84..716)
                     /locus_tag="PXB27_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018651773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2625 family protein"
                     /protein_id="HDL5347297.1"
                     /translation="MKNLEQLLDKQSAWLILRDWFTQAKNHYEILASYPEDAGSALVG
                     MQLSTRSPLGAMVYETGGVFIDHGWLRILGSGNEKLPRGLFDWNFGKTFKQSGEQPSH
                     LLVADDAIGGYFAINAGGIGSDLGKVYYYHPKAKKWENTQLGYSEFLGWALAGDLNHF
                     YQDLRWENWQTDVEKLQGHQVLDLTSKIAVPIERHYQGEFAPENMGYSVA"
BASE COUNT          212 a          164 c          128 g          219 t
ORIGIN      
        1 gatctaaatc gaaaatcaga aaatttcttt cgttctaaaa aaataagcta aacagcctat
       61 atttcagcct ctcttacaaa cacttaagcc accgaatatc ccatattttc aggggcaaat
      121 tcgccttggt agtggcgttc aatcggaacg gcgattttac ttgtaagatc cagcacttga
      181 tgcccttgta atttctctac atcggtttgc cagttttccc aacgcaaatc ctgataaaaa
      241 tggtttaaat ctccggctaa tgcccagcct agaaattctg aataacctaa ttgggtgttt
      301 tcccattttt tagcttttgg atgataataa taaactttgc caagatctga gccgattccg
      361 ccggcattaa tggcaaaata gccaccgatt gcatcatccg ctaccagcaa gtggcttggt
      421 tgttcgccag attgtttaaa agttttgcca aagttccaat caaataagcc tcgaggcaat
      481 ttttcgttgc ctgagcctaa aatgcgtaac caaccgtggt caataaatac gccaccggtt
      541 tcatacacca ttgcccctag cggtgagcga gtagaaagtt gcataccaac caatgcactg
      601 ccggcatctt cagggtaact cgctaaaatt tcgtaatgat ttttggcttg ggtaaaccag
      661 tctcttaaaa ttaaccaagc cgattgttta tctaataatt gttctaaatt tttcatttgc
      721 aaa
//
LOCUS       DANLUQ010000272          721 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.274, whole genome shotgun sequence.
ACCESSION   DANLUQ010000272 DANLUQ010000000
VERSION     DANLUQ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.274"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(78..716)
                     /locus_tag="PXB27_002569"
     CDS             complement(78..716)
                     /locus_tag="PXB27_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006252211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL5347298.1"
                     /translation="MELKHTIEQRKTIKAFNSTIKISRTELEEMVALSQLAPSKANLQ
                     PWRFVIIDVEILKAALSKNVAFNGPPCETASAIVVILADLQYEKLLGAIVDQSVETGC
                     LHKEFRERKFDFLLNLHNQATQAEIRDQVLIDTSLAAMQLMLIAKDKGYDTHAIGVFE
                     RPAVLKLLNIDAERYLPVMLLAIGKAANPALPSCRLPLEYTVSWNDGQGFKI"
BASE COUNT          232 a          145 c          110 g          234 t
ORIGIN      
        1 caatacaaaa ataaattaca acgctcaaat tttaacaatt ttttgccaaa aaattaagat
       61 atttaaccgc ttgcacctta tattttaaaa ccttgtccat cgttccaaga aaccgtatat
      121 tctagaggta aacggcaact tggcaacgct gggtttgctg ctttaccgat agctagtagc
      181 ataacaggca aataacgttc tgcatctata ttcaataatt ttaatacagc agggcgctca
      241 aacacgccta tcgcatgagt atcatagccc ttatctttgg caattagcat taattgcatt
      301 gccgccaaac tagtatcaat taatacctga tctctaattt ctgcttgagt tgcttggtta
      361 tgaagattta gtaaaaaatc aaatttacgt tcacgaaact ctttatgcaa acacccagtt
      421 tctaccgatt gatcaacaat tgcgcctaat aatttttcat attgcaaatc tgccaatatc
      481 accacaatag cagacgctgt ttcacaaggt gggccattaa aggcaacatt ttttgataat
      541 gctgctttaa gaatttcaac atcaataatg acaaaacgcc aaggttgcag atttgctttt
      601 gagggagcaa gctgggataa agcgaccatt tcttctaatt cagttctact aatcttaatt
      661 gttgaattaa aagctttgat agttttacgc tgttcaatcg tatgtttcaa ttccatctct
      721 t
//
LOCUS       DANLUQ010000273          703 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.275, whole genome shotgun sequence.
ACCESSION   DANLUQ010000273 DANLUQ010000000
VERSION     DANLUQ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.275"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            72..314
                     /locus_tag="PXB27_002570"
     CDS             72..314
                     /locus_tag="PXB27_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347299.1"
                     /translation="MFNLNLFKRPIELSTLESWSKFCDDVGKVAFIAIPPMIYSNYDL
                     CIKIGNISALLAITYGFLLSGRTIRQFIEKEKSRWD"
     gene            complement(474..662)
                     /locus_tag="PXB27_002571"
     CDS             complement(474..662)
                     /locus_tag="PXB27_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347300.1"
                     /translation="MLERGYVRIYQGKAYGWCAEQEDANTECPTALIVSLSNEIFEAT
                     GGDDISGAEKWEKYNSNF"
BASE COUNT          206 a          139 c          123 g          235 t
ORIGIN      
        1 tttcccgtgg ggagatactc tcccttcgga gcattgtgtc tcccttttta ttcacttaac
       61 tagaggacac aatgttcaat ctaaacttat ttaaacgccc tattgagctt tcaacgcttg
      121 aatcttggtc taaattctgt gatgatgtcg gtaaagttgc atttattgcg atacctccga
      181 tgatttatag taattatgat ctatgtatca aaataggaaa cataagtgct ttattggcaa
      241 ttacctatgg attcttatta tcaggcagga ctatccgtca atttattgag aaggagaaat
      301 caagatggga ttaacaatcg gcttatggat cattgctttt ataggctttg cactggtttt
      361 ttgggcaaat agcttactca aagagcaaga tgaacgcctt aaacagctaa aaaaatagct
      421 caactttaca ctcaatacgg cacatttggt gccgtatttc attcttatcc taattaaaaa
      481 ttagaattat atttttccca tttttcagcc ccacttatat catcacctcc agttgcctca
      541 aaaatttcat tagataagct gacaattaag gcggtagggc attccgtgtt tgcatcttct
      601 tgctctgcac accagccata ggcttttccc tgataaatac gaacataacc acgctctagc
      661 ataaaagggt gagaatcctt gtgattttga cgaaattttt gag
//
LOCUS       DANLUQ010000274          688 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.276, whole genome shotgun sequence.
ACCESSION   DANLUQ010000274 DANLUQ010000000
VERSION     DANLUQ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.276"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(76..177)
                     /locus_tag="PXB27_002572"
                     /pseudo
     CDS             complement(76..177)
                     /locus_tag="PXB27_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015587011.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            complement(257..>688)
                     /locus_tag="PXB27_002573"
     CDS             complement(257..>688)
                     /locus_tag="PXB27_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347301.1"
                     /translation="IFKRSNFMHLCGIKYTDGASEFFNAAISNNLDLAKMNVKSDGTT
                     FLKLSLLNSVSFLLTPEMCLTEGGIYLNIRFDKSLKTKKQIFALTLINQNHVFIPNSL
                     LNLKKMNDFPNGERVVSIKSIGLLTNEEIVFYGGCRRSDLI"
BASE COUNT          224 a          109 c          102 g          253 t
ORIGIN      
        1 tatgtcaccg ctatttttta tattttccta attatctagc tactttatct gtggattttt
       61 gcgtagattg ctgaacattc ttactaccac cactgcttga gagatattgc ccagttcttc
      121 tgctaaattt aataaaccgg tcttgtgttt aataaggaga ttgttagaat aaaacatgag
      181 agtttccttt tttgtttaga ttgaatttgg acactcatat tctaaacggg aaactctcac
      241 tttttagagt gaattgtcag atcaagtctg atcttctaca gccaccataa aaaacaatct
      301 cttcattggt taacaaacca atagatttta tgctaacaac tctttcccca tttggaaaat
      361 cattcatctt ctttaaatta agtagtgaat tgggaatgaa aacatgattt tggttaatta
      421 aggttaatgc aaaaatttgc tttttagttt taagagattt atcaaaacgt atatttaaat
      481 agatgccacc ttctgttaga cacatttcag gagtaagtaa aaaactaaca gaatttaata
      541 aagatagttt cagaaacgtt gttccatcac ttttaacatt cattttagca agatctagat
      601 tattagatat tgccgcattg aaaaattcag atgctccatc agtgtatttt attccacata
      661 aatgcatgaa attggagcgt ttgaatat
//
LOCUS       DANLUQ010000275          658 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.277, whole genome shotgun sequence.
ACCESSION   DANLUQ010000275 DANLUQ010000000
VERSION     DANLUQ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.277"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..>658)
                     /locus_tag="PXB27_002574"
     CDS             complement(<1..>658)
                     /locus_tag="PXB27_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AlwI family type II restriction endonuclease"
                     /protein_id="HDL5347302.1"
                     /translation="ADAPLETISEAFKLDISKVYVALSKDLGITIKSPEQAATYVNDE
                     RYRRFNTLIDKKFNDSVLIELLNCFEKRDDKRIEELVTDEAAIPTIFEYILGIIWYKV
                     SERQGNILDFMKLSLEANLLPKTHAAGGYADIIYEYEACTSYPKHSLLLEATLADGNN
                     QRRMEMEPVSRHLGDYRIRFNNPFDYSLFVSTYLDKNVISDFRYRKIIPYTRDEETIT
                     G"
BASE COUNT          195 a          129 c           92 g          242 t
ORIGIN      
        1 cctgtaatgg tttcttcatc tctggtgtat ggaattatct ttctatatct aaaatctgag
       61 attacatttt tatctaagta tgtgctaaca aataggctgt aatcaaacgg attattaaat
      121 ctaatcctat aatcgcctaa atgtcttgaa acaggctcca tttccattct tctttgatta
      181 tttccatctg caagggttgc ttcaagcaat aatgaatgtt tcggataaga ggtgcacgct
      241 tcatattcat atataatgtc tgcgtaacct ccagctgcgt gggtcttcgg caataaattg
      301 gcttctaatg ataacttcat gaaatccaaa atatttcctt gtctttcgct cactttgtac
      361 catataattc ccaaaatata ttcaaaaata gtaggtattg cagcctcatc tgtaacaagt
      421 tcttcaatcc ttttatcatc acgtttctca aaacaattta gtaactctat aagcacagaa
      481 tcgttaaatt tcttatcgat caaagtatta aatcttctgt atcgttcatc atttacataa
      541 gttgcagctt gttccggact cttaattgta attcctaaat ctttacttaa agctacatat
      601 actttgctaa tatctaactt gaatgcttct gatatagttt caagtggggc atcagccc
//
LOCUS       DANLUQ010000276          654 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.278, whole genome shotgun sequence.
ACCESSION   DANLUQ010000276 DANLUQ010000000
VERSION     DANLUQ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.278"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..296
                     /locus_tag="PXB27_002575"
     CDS             <1..296
                     /locus_tag="PXB27_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347303.1"
                     /translation="DTRYNKNGQGAVSERNIIDAKNRFKASHPYAKIISCVRGSETYV
                     TRPAKANATTKSVKEKQSQPQESSSLSSMLLGAAVTAGVGFVANKWFSKKDNT"
     gene            324..>654
                     /locus_tag="PXB27_002576"
     CDS             324..>654
                     /locus_tag="PXB27_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347304.1"
                     /translation="MAGLLPPYFKKKNSDHYKDAVKQFGKEQVDEVIAQCDELESWVK
                     EMPTKQLIMYVGCFHTFNKLSQSKAYVQENFPNVLENPFLFQTLSAASHNELKINRGY
                     KFKEKMLD"
BASE COUNT          243 a          106 c          119 g          186 t
ORIGIN      
        1 cagataccag atataacaag aatgggcaag gtgctgtttc tgaaagaaat attatagatg
       61 ctaaaaatcg ttttaaagcg agccatccat acgcaaaaat tatttcttgt gttagagggt
      121 ctgagacata tgtaacacgt ccagcaaaag ctaatgctac aacaaaatct gtcaaggaaa
      181 agcaatctca accacaagaa agttcatcgc tcagttcaat gttacttggt gcggctgtaa
      241 ctgctggggt tggttttgta gccaataaat ggtttagcaa aaaagataac acttaattta
      301 tctctaacca atgtggagta aaaatggctg gattattacc accttatttc aaaaagaaaa
      361 atagtgatca ctataaagat gctgtaaaac aatttgggaa agaacaagtt gatgaggtta
      421 tagcacagtg tgatgaactt gaatcttggg taaaagaaat gccaacaaaa caacttatta
      481 tgtatgttgg ttgtttccat acttttaaca aattatctca atctaaagct tatgttcaag
      541 agaactttcc aaacgtactg gaaaacccgt ttcttttcca aacactttcg gcagcgtctc
      601 ataatgaact taaaataaat cgaggatata aattcaaaga aaaaatgctt gatg
//
LOCUS       DANLUQ010000277          648 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.279, whole genome shotgun sequence.
ACCESSION   DANLUQ010000277 DANLUQ010000000
VERSION     DANLUQ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.279"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(19..303)
                     /locus_tag="PXB27_002577"
     CDS             complement(19..303)
                     /locus_tag="PXB27_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010128390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347305.1"
                     /translation="MANITLSHITPKHFFETVRLLKMQEHRIYIGVDSIEVRFKQKRF
                     ESKKETDKRLEQLEHFMLFLQEIINSDIPIDLDEFIYTYHRSVRRGFYRR"
     gene            complement(321..482)
                     /locus_tag="PXB27_002578"
     CDS             complement(321..482)
                     /locus_tag="PXB27_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007525341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL5347306.1"
                     /translation="MEKIENSSNLVLKLSLENQMLILESLEHPKEPNLVMLEALKKYS
                     LYVAKTSNK"
BASE COUNT          202 a          112 c          103 g          231 t
ORIGIN      
        1 cactcctctt tcacatcact aacgtcgata aaaacctctc cttactgacc tgtgataggt
       61 gtatataaac tcatctaaat ctataggaat atctgaatta atgatttctt gtagaaagag
      121 cataaaatgc tctaactgtt ctagtctctt atctgtttcc ttttttgact caaaacgctt
      181 ttgcttaaaa cgaacttcta tcgaatctac accgatgtaa attcgatgtt cctgcatttt
      241 gagtagtcgg acagtttcaa aaaaatgttt aggggtgatg tgagaaaggg ttatatttgc
      301 catagtttag cttgaaaacg ctatttatta cttgttttag caacatacaa agaatacttt
      361 tttaaagcct ctagcattac taaattaggc tctttaggat gttctaagct ctcaagaatt
      421 agcatttgat tttcaagaga aagctttaga acaagatttg aactattctc tattttttcc
      481 attttaatta tctcattaga tgactttcgc ataacaacag gttatgtgta aattctcgtg
      541 cagtgggaca agcccgattg tactgcactc gaattgtaac ataacctgta ggacattatg
      601 cgaaaattaa taatataagt ttttcatcaa tcggcggtat tattgaat
//
LOCUS       DANLUQ010000278          646 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.280, whole genome shotgun sequence.
ACCESSION   DANLUQ010000278 DANLUQ010000000
VERSION     DANLUQ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.280"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(184..>646)
                     /locus_tag="PXB27_002579"
     CDS             complement(184..>646)
                     /locus_tag="PXB27_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347307.1"
                     /translation="GDRNYQLYIDLATGELVSKLGKGLTTREFDAAVKAFENAFNAKD
                     TAENTSPAESKETEQENKQETTKLVITKPTLKGSEKQIAWADEIINLFIAKIGDTSIP
                     VTATQEQITQAQAMVDNFFAMTNAGKWIEKFKGLNDSSEPKQFWLSVKYGW"
BASE COUNT          146 a          129 c          118 g          253 t
ORIGIN      
        1 tatagggtat ctcaagatta ttgtctgtac gtttgttggc acatcgtcac cataagtagc
       61 agcgacaatt tgcccgataa tgcgcataat ttgtggtgtt acttgtgtag tcataataat
      121 ctccagttat ctgtttgttt tagccgtaag cctttgctct tacggcttat ttgttatctc
      181 aatctaccat ccatatttta cggataacca aaattgcttt ggttcgctac tatcatttag
      241 acctttaaat ttttcgatcc actttccagc gtttgtcatt gcaaaaaaat tatctaccat
      301 tgcttgagct tgagtaattt gttcttgcgt tgctgttact gggatagagg tatcaccaat
      361 cttcgcaata aaaagattga tgatttcgtc tgcccaagcg atttgttttt cagagccttt
      421 taatgttggt tttgtgatta caagcttagt tgtttcttgt ttgttctctt gctctgtttc
      481 tttgctttct gctggcgatg tattttcagc ggtgtcttta gcattaaaag cgttctcaaa
      541 tgcttttacg gcagcgtcga actctcttgt tgttaatcct ttgccaagtt tgctcactaa
      601 ctcacctgtg gctaagtcaa tgtacaactg ataattccga tcacct
//
LOCUS       DANLUQ010000279          646 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.281, whole genome shotgun sequence.
ACCESSION   DANLUQ010000279 DANLUQ010000000
VERSION     DANLUQ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.281"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(184..>646)
                     /locus_tag="PXB27_002580"
     CDS             complement(184..>646)
                     /locus_tag="PXB27_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347308.1"
                     /translation="GDRNYQLYIDLATGELVSKLGKGLTTRAFDAAVKAFENAFNAKD
                     TAENTAPVESKETEQENKQETTKLVITKPTLKGSEKQIDSANEIINLFIAKIGDTSIP
                     VTATQEQITQAQAMVDNFFAMTNAGKWIEKFKGLNDNSEPKQFWLSVKYGW"
BASE COUNT          147 a          127 c          119 g          253 t
ORIGIN      
        1 tatagggtat ctcaagatta ttgtctgtac gtttgttggc acatcgtcac cataagtagc
       61 agcgacaatt tgcccgataa tgcgcataat ttgtggtgtt acttgtgtag tcataataat
      121 ctccagttat ctgtttgttt tagccgtaag cctttgctct tacggcttat ttgttatctc
      181 aatctaccat ccatatttta cggataacca aaattgcttt ggttcgctat tatcatttag
      241 acctttaaat ttttcgatcc actttccagc gtttgtcatt gcaaaaaaat tatctaccat
      301 tgcttgagct tgagtaattt gttcttgcgt tgctgttact gggatagagg tatcaccaat
      361 cttcgcaata aaaagattga tgatttcgtt tgccgaatca atttgttttt cagagccttt
      421 taatgttggt tttgtgatta caagcttagt tgtttcttgt ttgttctctt gctctgtttc
      481 tttgctttct accggcgctg tattttcagc ggtgtcttta gcattaaaag cgttttcaaa
      541 agcttttacg gcagcatcaa acgctctggt tgttaatcct ttgccaagtt tgctcactaa
      601 ctcgcctgtg gctaggtcaa tgtacaactg gtaattccga tcacct
//
LOCUS       DANLUQ010000280          645 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.282, whole genome shotgun sequence.
ACCESSION   DANLUQ010000280 DANLUQ010000000
VERSION     DANLUQ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.282"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..>645
                     /locus_tag="PXB27_002581"
     CDS             <1..>645
                     /locus_tag="PXB27_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018650961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL5347309.1"
                     /translation="LSLEGFKMKVVHNGLEGLNELESNQYDLVLLDVMMPVLSGVKTL
                     KRIRQKYSLPVLMLSARDDQIDRILGLELGADDYVPKPFNNRELVARMKAILRRTTSR
                     PASTDHSEQNYTENISKSLQFAGVELHSGRQQAYYQGCDLDLTGTEFALLQMLMRNSG
                     EILSRELLSLKILGKNLTPYDRAIDMHISNLRKKLPEREDGLPWFKTLRGRGYL"
BASE COUNT          222 a           94 c          129 g          200 t
ORIGIN      
        1 actttcatta gaaggattta aaatgaaagt tgtgcataat ggtttagaag gcttaaatga
       61 gttagagtct aatcaatatg atttagtatt actagatgtg atgatgccgg tattaagtgg
      121 cgtgaaaaca ttaaaacgta tacgacaaaa atactcatta cccgttttaa tgttaagtgc
      181 aagagatgat caaattgata gaatcttagg tttagaatta ggggctgacg attatgtacc
      241 gaaacctttt aataatcgtg aactagtcgc tagaatgaaa gcgatacttc gaagaacaac
      301 ctctcgccca gcttcaacag accattctga acagaattat actgaaaaca tctctaaatc
      361 attacaattt gccggagttg aattacattc aggacgtcag caggcttatt atcaaggatg
      421 tgatcttgat ttaacgggga ctgaatttgc attattacaa atgttaatgc gaaactcagg
      481 ggagattttg tccagagaat tattgagttt aaaaatttta gggaaaaatt taacgcctta
      541 tgatcgtgca attgatatgc atatttctaa tcttcgtaaa aaattacccg aacgggaaga
      601 tggattacct tggtttaaaa ccttaagagg tcggggttac ttatt
//
LOCUS       DANLUQ010000281          636 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.283, whole genome shotgun sequence.
ACCESSION   DANLUQ010000281 DANLUQ010000000
VERSION     DANLUQ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.283"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..>636
                     /locus_tag="PXB27_002582"
     CDS             <1..>636
                     /locus_tag="PXB27_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDL5347310.1"
                     /translation="PQMKKSKDYKDFLDALEKGDVDKADKIYNTKFSQFSKSFGHEME
                     KNLEKNRIPPIYQGVPFDSFDLYQHYSYGNGEALNLSSVGLSNKIRPLVNKDKAFGKS
                     NGSIESRFISQYKNEGRTSFSNAYDFTKEASGIADPLWALGTATISGELKDIQIFPGG
                     IARGNIHYSIRDRFTDPFDTFNFTKGEWNPNGTPYIIQDSWIKSVRFSIDK"
BASE COUNT          229 a           92 c          122 g          193 t
ORIGIN      
        1 gtccacaaat gaaaaagagt aaagactaca aagacttttt ggatgcttta gaaaaagggg
       61 atgttgataa agcagataaa atatacaata cgaagttttc acagttttct aaatcttttg
      121 gtcatgaaat ggaaaagaat ttagagaaaa atcgtatacc tcctatttat cagggagttc
      181 catttgattc ttttgactta tatcagcatt attcttatgg taatggagag gcattaaatc
      241 tttcatccgt tggattaagt aataaaattc gtcctctagt gaataaagat aaagcatttg
      301 gtaaatccaa tggaagtata gaaagccgat ttatttcgca atataaaaat gaaggacgca
      361 catctttctc caatgcttat gattttacaa aagaagcatc gggtattgca gatccgttat
      421 gggcgttagg aacagctacc attagtggag aattgaaaga tatacaaatt tttcccggtg
      481 gtattgctag gggaaatatc cattatagta tcagggacag attcactgac ccatttgata
      541 cattcaattt tactaaagga gaatggaacc caaatggaac accttatatt attcaagata
      601 gctggataaa atcggtacga ttttcgatag acaaga
//
LOCUS       DANLUQ010000282          632 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.284, whole genome shotgun sequence.
ACCESSION   DANLUQ010000282 DANLUQ010000000
VERSION     DANLUQ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.284"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            1..632
                     /locus_tag="PXB27_002583"
                     /pseudo
     CDS             1..632
                     /locus_tag="PXB27_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484596.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          220 a           92 c          114 g          206 t
ORIGIN      
        1 ttttccttaa taatgcacaa cgaacagcct tttcatatgt aactcattac attaagaaac
       61 attcctatgc ctatagtaag gcagacaata ttcaagaatt attagtaaag tttttctcta
      121 gaaagcaacc tgttatgcaa agtgcattta atcatcaaaa aaggattgtt aagtggttat
      181 gggctgaatt ttgggataag aatattatct cgatgacact tagaattgat ggttatagaa
      241 atgctaactt atggcattat ttacgttatc aatcacggat tgaggtattg tataaaatag
      301 caaatgaaca cccgaatcta ctaccattat tagcctatgt taaacctgag tactggaaat
      361 gctcggactt atttagttat caaaattggg taagaagccg tgataaacag ccttttattt
      421 tgtatgctaa atgccaagat aagatgttat gtgaatcaaa ggggcaatgg agatggttaa
      481 aaaaacagtc gagaatagtt gtagatcaat gttttctaca tgttaatcaa tggcagtaat
      541 taacagaaat tcaaatgcct ttcaagatcc cagtatgtgt catatgtttc atacttacta
      601 attttagcat gatcagctgt gagttagatc ga
//
LOCUS       DANLUQ010000283          617 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.285, whole genome shotgun sequence.
ACCESSION   DANLUQ010000283 DANLUQ010000000
VERSION     DANLUQ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.285"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..513
                     /locus_tag="PXB27_002584"
     CDS             <1..513
                     /locus_tag="PXB27_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL5347311.1"
                     /translation="PSWFILAIIMTLRCTGIRRGQLVKLKIGDVNLSNRTIYISPEIN
                     KNHDYHIVPISDSLYPHIERLIQELKIHKQTDDCQLFNLNLFSRYTRRKGQPMSQNQL
                     THIFRHISDLVGFTSSPHRFRHTVATQLMKKPENVYVVKKLLGHKDISVTLGYIEYDV
                     EMLRDTVNLL"
BASE COUNT          215 a           98 c          108 g          196 t
ORIGIN      
        1 cctagctggt ttattcttgc tatcattatg acattgagat gcacaggaat taggagaggg
       61 caattggtta aactgaaaat cggtgatgtt aatttatcaa ataggacgat ctatatttca
      121 ccagaaatta ataaaaacca tgattatcat atagtaccga tatccgattc actttatccc
      181 catatagaaa ggctgattca ggagttaaag atccataaac aaacggatga ttgccagtta
      241 tttaacctca atttattttc tcgttatact cgtaggaaag gtcagccgat gagccaaaac
      301 cagcttactc atatatttag gcatatttcc gatttggttg gcttcacgtc atcaccacat
      361 agatttcggc atacagtagc tactcaattg atgaagaaac ctgaaaatgt ttatgtagtt
      421 aagaaattat taggtcataa agatatcagc gttacattag gatatattga atacgatgtg
      481 gaaatgctac gagacaccgt gaacttactc taataaaaga gaaatgagag aaaactattc
      541 tctcatttat tgtgataaat aggaaacttc tgtcttattt tcagatgaat tgattttgag
      601 aactttatca aaatata
//
LOCUS       DANLUQ010000284          609 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.286, whole genome shotgun sequence.
ACCESSION   DANLUQ010000284 DANLUQ010000000
VERSION     DANLUQ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.286"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            285..>609
                     /locus_tag="PXB27_002585"
     CDS             285..>609
                     /locus_tag="PXB27_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347312.1"
                     /translation="MKEANMTNKFTGYKKLSINDLKKFEYNLEHIRNAAIFFKDHFLS
                     KEMIYSTEKESTSVIFQRSNFMHLCGIYYEPGASEFFNLALLKQIDLNKVYIKNDGTT
                     FQKLSV"
BASE COUNT          225 a           87 c          106 g          191 t
ORIGIN      
        1 aatgatttta tagtatattt tttacaggtt agattacaaa tataatatat actagcttga
       61 ctttaggaga taatcattat aatctgattg tcaaggtggc gttaccttaa taacgcaatg
      121 agttgttggc tcatcgtcaa tctgatatta atctaggatg taaaggccta gcggtggcag
      181 gcaccagaaa actctgatac taatctagga tgtaaaggcc tagcggtggc agacaccaga
      241 ataaaccaac atttaggccc acttaattgt gggcttaata ctttatgaaa gaagcaaata
      301 tgactaataa atttactgga tacaaaaaac taagcattaa tgacttgaaa aaatttgagt
      361 ataatttaga gcatataaga aatgccgcaa tctttttcaa agatcatttt cttagtaaag
      421 aaatgatata tagtactgaa aaagaaagta catccgttat ctttcaaaga agcaatttta
      481 tgcacttgtg cggaatatat tatgaacccg gtgcgagtga attttttaat cttgccttat
      541 taaaacaaat agacctaaat aaggtttata taaaaaatga tggcacaaca ttccaaaagt
      601 tgtctgttt
//
LOCUS       DANLUQ010000285          607 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.287, whole genome shotgun sequence.
ACCESSION   DANLUQ010000285 DANLUQ010000000
VERSION     DANLUQ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.287"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            211..>607
                     /locus_tag="PXB27_002586"
     CDS             211..>607
                     /locus_tag="PXB27_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347313.1"
                     /translation="MASALGAFLLLLVASSYADFYYTQTKQKEQLYLQAEAHQLLNYF
                     QQHFQHIGYQGSHRTDSNFDLFQQAGKSLNIVHKNCIIGFYDLNQDGCLGKRKTKTAA
                     CKLGTVNNTREVLKEIVGFKVENNEIYTFS"
BASE COUNT          222 a           88 c          111 g          186 t
ORIGIN      
        1 tattaaaaaa taaaagttta tatccgaaat cctttattaa tgttgatggg gtaagtggtc
       61 aattagaatc aaaatgtatt aagctcagtt taaataaaga aaatgaaatt ttgcaatttg
      121 aacaattagg acgggtttat gtggcagcaa aaaataaacg tagcacctgc aaagattagt
      181 ttatctgctt ttagcctaat tgaagtatta atggctagtg cattaggggc gtttttatta
      241 ttgcttgttg cttcaagtta tgccgatttc tattacacac aaaccaaaca aaaagaacag
      301 ctctacttgc aagcagaagc tcaccagtta ctaaactact tccaacagca ttttcaacac
      361 attggctatc aaggctctca cagaacagac agtaattttg atttatttca gcaagcaggg
      421 aaaagcctga atattgtgca taaaaattgc attattggat tttacgattt aaaccaagat
      481 ggctgtttgg gaaaacgtaa gacaaaaacg gccgcttgta agttaggaac agtgaataat
      541 accagagagg tgttaaaaga aattgttggc tttaaagtag aaaataatga aatttatact
      601 ttttcat
//
LOCUS       DANLUQ010000286          604 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.288, whole genome shotgun sequence.
ACCESSION   DANLUQ010000286 DANLUQ010000000
VERSION     DANLUQ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.288"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..549)
                     /locus_tag="PXB27_002587"
     CDS             complement(<1..549)
                     /locus_tag="PXB27_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347314.1"
                     /translation="MSALINLFYLYDPWFFHFLRMSFVVGLVACGYLVFQIIKGKSNG
                     IIIPKDSLIAIIALIGFSIIPLLINSTKEIGVLAMYVKMLILFVFGIAIYNVFYHNQQ
                     GKTLFVRDLKIGIAIQWICGVTALLGLPFMVDFLLSSNAMMPRFFGSEQEYRLYNITS
                     SAFFQLSIFYLMLLHFLLAYNKK"
BASE COUNT          239 a          105 c           90 g          170 t
ORIGIN      
        1 ttttttatta tatgccaata aaaaatgtaa tagcattaaa taaaaaatgc ttaattggaa
       61 aaaggcggaa gaagtgatgt tatataaacg gtattcttgt tccgaaccaa aaaaacgtgg
      121 catcattgca ttgctggata ataaaaaatc caccataaac ggcaaaccta ataaggctgt
      181 cacaccacaa atccattgaa tagcaatgcc gatttttaaa tcacgaacga ataaagtttt
      241 accttgttgg ttatgataaa aaacattata aatcgcaatc ccgaacacaa acaaaatcag
      301 cattttgaca tacattgcca gcacaccaat ttcttttgta ctgttaatca ataatggaat
      361 aatgctaaag ccgattaatg caataattgc aatcaggcta tctttaggaa taataatgcc
      421 attcgatttg cccttgatta tttggaaaac caaatagcca caagcaacaa gcccgactac
      481 aaacgacatt cttaaaaaat ggaaaaacca cggatcgtaa agataaaata agttaatgag
      541 tgctgacatt tcagacctat tatgaatttc gctgagtaaa attacgttta attgccagta
      601 attt
//
LOCUS       DANLUQ010000287          594 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.289, whole genome shotgun sequence.
ACCESSION   DANLUQ010000287 DANLUQ010000000
VERSION     DANLUQ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.289"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
BASE COUNT          172 a           90 c          110 g          222 t
ORIGIN      
        1 ttatgctcct tttatcatat ctcaaaaggc attttaggta aataagcata aataaaaaaa
       61 tagatacttt atatctaaag atattcgtgc tatcttaaac actgtcctac aaattgtaga
      121 ggtgcgaatt caataagtat ttcttctgag tggaaaacga tgaaggggaa ggaaaggtga
      181 atttgccgaa atcaattaag cgtcatctta attggttggg gtcgttgtcg aaagaaacga
      241 cactgtcgta gtaaatgcta cggagtgcta ctgttagggt gggtttcaga tatttcacat
      301 ttgccttcct ttcatattta tttttataaa tatcccagta gatctcttgt ttggctattc
      361 ttatttatac aaattataca cagtcatttt agaggtttta tgaatctagt tgattattct
      421 acgtcacttt ggtcggtatt gccggcactg attgcgctaa tattggcaat tgtgacacgc
      481 aaggtgatta tttcacttag catcggtatt ctctttggtg catttatgct tactaatagc
      541 aattttcttg atgcacttac ttatattaaa aatagcgttg tttctttagt ttat
//
LOCUS       DANLUQ010000288          570 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.290, whole genome shotgun sequence.
ACCESSION   DANLUQ010000288 DANLUQ010000000
VERSION     DANLUQ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.290"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..>570)
                     /locus_tag="PXB27_002588"
     CDS             complement(<1..>570)
                     /locus_tag="PXB27_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347315.1"
                     /translation="CSLWFLSACFNESYDFRGKEFEEPVQAILVESDKIYFIGSTIDA
                     IFDKTLIDNTKASDVIYFLNKLLQSPYGRKISNHLITLAIYDENIQIQYSPQFIAKNL
                     TMSDIQALQKLGFQFSHSKEYLWKSYAGLGGKITQFKNRKEILEKYKFKQPIIAKAKS
                     YQKSIEFSFDDTISVIAAPITIPASIILFV"
BASE COUNT          182 a           91 c           91 g          206 t
ORIGIN      
        1 tacaaataaa ataatagatg cgggaatcgt aattggtgct gcaatgaccg aaatggtgtc
       61 atcaaagcta aattctatgc ttttctgata acttttcgct ttagcgatta taggttgctt
      121 aaatttatac ttttctagta tttccttgcg atttttaaac tgtgtgattt taccaccaag
      181 acctgcatag cttttccata gatattcttt tgaatggcta aattgaaaac ctaacttttg
      241 tagagcttga atatcgctca tggtgagatt ttttgcaata aattgcggac tgtactgaat
      301 ttgaatattt tcatcataaa tggctaaagt aattaggtga ttactgatct tccttccata
      361 cggactttga agaagtttat tcaaaaaata aataacatct gaagcttttg tattatctat
      421 cagcgtttta tcaaatatcg catcaattgt ggaaccaatg aaataaattt tatctgattc
      481 aaccaaaatt gcttgtacag gttcttcaaa ttcttttcct ctaaaatcat aactttcgtt
      541 aaagcatgcg gagagaaacc atagtgagca
//
LOCUS       DANLUQ010000289          565 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.291, whole genome shotgun sequence.
ACCESSION   DANLUQ010000289 DANLUQ010000000
VERSION     DANLUQ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.291"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..544)
                     /locus_tag="PXB27_002589"
     CDS             complement(<1..544)
                     /locus_tag="PXB27_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL5347316.1"
                     /translation="MGRPELERAVLSVQNQTYPCKHYVFVDGEKYHQQAADILKNFPS
                     VIVTYLPMNTGADGWVNSSINAIAPFLVKEDIICYLDDDNWFEPTHVESGVHALLEMQ
                     SDYAYSLRNFYDLDGEFLCIDTIESLGEYNCVYQEPLECNYQLGGVKGVLSINLNDCQ
                     HIDTNCYFIRSEIARLISTAW"
BASE COUNT          208 a          117 c           74 g          166 t
ORIGIN      
        1 accaagccgt actgataagg cgagctatct cactacgaat aaaatagcaa ttcgtatcaa
       61 tatgctgaca atcattcaga tttattgata aaacaccttt aacaccacct aattgataat
      121 tacattctaa tggttcttga taaacgcaat tatattctcc caacgattca atcgtatcaa
      181 tacacaaaaa ctcgccatct aaatcataaa aattacgtaa agagtaagca taatcagatt
      241 gcatttctaa taaagcatgc acaccagatt caacgtgagt aggttcaaac caattatcat
      301 catccagata acaaataata tcttctttca ctaaaaatgg tgcaattgca ttaatgctgc
      361 tatttaccca accatcagcc ccggtattca ttgggagata agtaacaata acacttggaa
      421 aattctttaa aatatcagcc gcttgttggt gatatttctc tccatcaaca aaaacataat
      481 gtttacaagg ataagtctga ttttgtacgc ttaaaacagc tctttctaat tcaggacgac
      541 caattgttga actgataaca gctac
//
LOCUS       DANLUQ010000290          554 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.292, whole genome shotgun sequence.
ACCESSION   DANLUQ010000290 DANLUQ010000000
VERSION     DANLUQ010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.292"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..>554
                     /locus_tag="PXB27_002590"
     CDS             <1..>554
                     /locus_tag="PXB27_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347317.1"
                     /translation="KVLLTAELKQTDFLKRNQSKGIVIEGGSDEWFDEWKKGCIEFIE
                     LAKNQDFLHKIILNKVMWTNQLANGEKFIEDDKIEKANTFLERQYEFISKFIPQENII
                     TYSSELFKANKDYMLEQLSIPTLRKDSNKLINKEQIDDSSRDYLDYIKHRIDELYNLN
                     YQQISALDMYQTEKKDYELKLNTL"
BASE COUNT          224 a           62 c           98 g          170 t
ORIGIN      
        1 taaagttctg ctgacagcag aacttaaaca gactgatttc ctaaaaagaa atcagagcaa
       61 aggtattgtt attgaaggag ggagtgatga atggtttgat gaatggaaga agggttgcat
      121 tgaatttatt gagttagcaa aaaatcaaga ttttctccat aaaattatct tgaataaggt
      181 gatgtggaca aaccaattag ctaatggtga gaagtttatt gaagatgata aaattgagaa
      241 agctaacacc tttttagaaa ggcagtatga gtttatcagc aaatttattc ctcaggaaaa
      301 tatcattact tattcaagtg agttattcaa agccaataaa gattatatgt tagaacaatt
      361 atcaatccct actttaagaa aggatagtaa taagttaatt aataaagaac aaattgatga
      421 tagttctcga gattatctag actatattaa gcacagaatt gatgaattat ataatcttaa
      481 ctatcaacaa atatcagcac ttgatatgta tcaaactgag aaaaaagatt atgagcttaa
      541 attgaatact ttaa
//
LOCUS       DANLUQ010000291          552 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.293, whole genome shotgun sequence.
ACCESSION   DANLUQ010000291 DANLUQ010000000
VERSION     DANLUQ010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.293"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
BASE COUNT          195 a           99 c           78 g          180 t
ORIGIN      
        1 taagtatata tttttttcta attctgtatt ctcatattca tgaatatacg ttttaacaaa
       61 aagattatac tcttttagta catcaactaa ttcatcaggt aaattttcta acttccatct
      121 tcgtttttct aaaatcgaaa agtaatactg gtcatcgtct aaaatctcaa tatatgtact
      181 tccaaagaga acttttgtat aaggcgtatc atgatagatc gtgacatcag ccttcaattt
      241 gtactcgcct tccatgcatt ccccaaaatg gtattgacta aaaacaatgg ttatgatata
      301 atgacatggc tttgctcaat aggtaatcag accactttga aagctcggtc cttttcaaaa
      361 gaaaaggtct tactaattac tcacaaaatc tggagaacaa tatgaaaaac actacagcgc
      421 aacaaaagcc aatgaaagtt acagctaaac atattagaga atctactact cgttgttaag
      481 agcttagctt tgatataaaa agaccaactt tttatatcgg cgtagtaggt ctaatttgag
      541 cctacgaaat ga
//
LOCUS       DANLUQ010000292          550 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.294, whole genome shotgun sequence.
ACCESSION   DANLUQ010000292 DANLUQ010000000
VERSION     DANLUQ010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.294"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..401
                     /locus_tag="PXB27_002591"
     CDS             <1..401
                     /locus_tag="PXB27_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347318.1"
                     /translation="FQGEIAIIMEVNRNKGVIQKHRFYEPDFTTIKNGKVIGLTPEFQ
                     LLRLPKPEFADALINLAKRVSIALPTPFARVDLYWDGKQAKVGEITLTPFYGYPYKWT
                     LSQDIIMGRMWLEALDRMGKKLEDSFDWGN"
BASE COUNT          173 a           81 c          113 g          183 t
ORIGIN      
        1 tattccaggg tgaaattgca attattatgg aagtaaatcg taataaaggt gtaattcaaa
       61 aacatcgatt ttacgagcct gattttacca ctattaagaa tggaaaagtt attggcttaa
      121 ctccagaatt ccaattatta cgtttaccta aacctgaatt tgccgatgct ttgattaatc
      181 tagcaaaacg agtatctatt gcattaccta ccccatttgc tcgagtggat ttatattggg
      241 atggaaagca agctaaagtt ggagaaatta cattaactcc tttctatggt tatccataca
      301 aatggacatt atcacaggat atcataatgg gaagaatgtg gttggaggca ctagatagaa
      361 tggggaaaaa attggaagat tctttcgatt ggggaaatta atgtgaaatc aacaaaggta
      421 tcgttctttc aggctatagc gattagtttt ggtgctatta ttggttgggg ggcttatgtg
      481 atgcctggag acctttttct ttcaaaaggt actctattaa gctcttctct tgctattatt
      541 ttaggaacaa
//
LOCUS       DANLUQ010000293          550 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.295, whole genome shotgun sequence.
ACCESSION   DANLUQ010000293 DANLUQ010000000
VERSION     DANLUQ010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.295"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..176)
                     /locus_tag="PXB27_002592"
     CDS             complement(<1..176)
                     /locus_tag="PXB27_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase family
                     protein"
                     /protein_id="HDL5347319.1"
                     /translation="MNIKATLRKARLVIGKAILDSKNTDLILSEIPNKILFLRHDGKI
                     GDYVVSSFVFREIK"
     gene            224..>550
                     /locus_tag="PXB27_002593"
     CDS             224..>550
                     /locus_tag="PXB27_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase family
                     protein"
                     /protein_id="HDL5347320.1"
                     /translation="MKSLVIKLFGTKKEKKTFKFSKIESVLLKPIGDAIGDAVVHIAH
                     LEQIKKALPNVKIAVLVTSRNKLIFSQANVVDMILEDKVSTYLSQRGHWDLYLDFKPT
                     FTTKSII"
BASE COUNT          197 a           94 c           78 g          181 t
ORIGIN      
        1 ttttttattt cacggaagac aaaggaacta acgacataat caccaatttt accatcgtga
       61 cgaagaaata gaattttatt gggaatttcc gataatatca gatcagtatt tttactatct
      121 aaaatggctt tacctatcac caatcttgct tttcttaacg ttgctttaat attcattttt
      181 acttactata accaatcatt aaaagaatca acctattata gagatgaaat ccttagttat
      241 aaaacttttt ggtacaaaaa aagagaaaaa aaccttcaaa tttagcaaaa tagaatccgt
      301 attactcaaa cctatcggag atgctattgg cgatgctgtt gtgcatatag ctcatttgga
      361 gcaaattaaa aaagctctcc caaatgtaaa aatagcggtt ttagttacat ctcgtaacaa
      421 attgattttc tctcaagcaa acgtggtaga tatgatctta gaagataaag tatccactta
      481 tctctcgcag cggggacatt gggatcttta tttagatttc aaaccgacat ttaccacaaa
      541 aagtattatt
//
LOCUS       DANLUQ010000294          535 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.296, whole genome shotgun sequence.
ACCESSION   DANLUQ010000294 DANLUQ010000000
VERSION     DANLUQ010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.296"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..193)
                     /locus_tag="PXB27_002594"
     CDS             complement(<1..193)
                     /locus_tag="PXB27_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL5347321.1"
                     /translation="MSFEQLLHLFFLERNHRPATIRSYQNVIAQIVKFYPDKEPEQIS
                     KLDLLEWRKYCLNKMSPITW"
     gene            343..>535
                     /locus_tag="PXB27_002595"
     CDS             343..>535
                     /locus_tag="PXB27_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347322.1"
                     /translation="MKEWYTLKELLDVGGLPSTPQGIILYAKKDKWRRRRVSGVKGNV
                     FEYYVYDMPESVQTSLGVTK"
BASE COUNT          188 a           77 c           92 g          178 t
ORIGIN      
        1 tccaagtaat tgggctcatt ttattgaggc aatactttct ccattctaat aagtcaagct
       61 tagatatttg ttcaggttct ttatctggat aaaatttaac aatctgcgct attacatttt
      121 gataagaacg tatagtggca ggtctatgat tccgttctaa gaaaaataaa tgcaataatt
      181 gttcaaaact catacaaaac ctcatttaga taagcgatct tcaaatagca ctcaatttaa
      241 tgtagactag atctccttga attagccttg atcttttaag ttaaagttaa ttcaatttaa
      301 gttaaattta acttaaattt aaaataaagt caatgaggta atatgaaaga atggtatact
      361 cttaaagagc ttttagatgt aggagggctc ccttcaacac cacaaggtat aatcttatat
      421 gctaaaaaag ataaatggcg tagacggcgt gtctctggag tgaaaggtaa tgtttttgaa
      481 tactacgtgt atgatatgcc agaatctgta caaactagtt taggagtaac taagg
//
LOCUS       DANLUQ010000295          527 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.297, whole genome shotgun sequence.
ACCESSION   DANLUQ010000295 DANLUQ010000000
VERSION     DANLUQ010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.297"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(184..525)
                     /locus_tag="PXB27_002596"
     CDS             complement(184..525)
                     /locus_tag="PXB27_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HDL5347323.1"
                     /translation="MKAILCQRAKQLRTNMTEAEKVLWQALRAKRFLAIKFKRQQVIG
                     NYIVDFVCFSAKLVIELDGSQHLDQQNYDNLRTHFLNGQGFRVLRFWNNQIHSDLEAV
                     LDTIFYEITRG"
BASE COUNT          177 a           96 c           80 g          174 t
ORIGIN      
        1 cacaagggga gagggttatt ctttcaaaaa ttcaaaacaa ctaaaaaatt agcattctcc
       61 ttttgtagga gaaaatgttg ttctttcaaa attctaaaat aaccgcaaaa ttaactctcg
      121 ccccttgtgg gagagagaca gataaatcat tcgttcagaa tgatttatca gagagagggg
      181 gaattaccct ctcgtaattt cataaaaaat tgtatctaaa acagcctcta gatcgctatg
      241 aatttgatta ttccaaaatc ttaaaaccct gaacccctga ccgtttaaaa aatgagttcg
      301 tagattatca tagttttgtt gatctaaatg ttgtgaacca tctaattcaa taactaattt
      361 cgctgaaaag caaacaaaat cgacaatata gttacctata acttgttgcc gtttaaattt
      421 tatcgctaaa aatcgttttg ctcttaatgc ttgccaaaga accttctctg cttctgtcat
      481 attagtccga agctgtttag ctctttggca taaaattgct ttcattt
//
LOCUS       DANLUQ010000296          511 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.298, whole genome shotgun sequence.
ACCESSION   DANLUQ010000296 DANLUQ010000000
VERSION     DANLUQ010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.298"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            32..>511
                     /locus_tag="PXB27_002597"
     CDS             32..>511
                     /locus_tag="PXB27_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL5347324.1"
                     /translation="MIHNKISLDISSEQLADIRQQMLKFAQLQLQDIALAEDMVQEAF
                     VNAIKNIDNFQRKAALKTWIFAILKNKIIDYLRKKDRIVLESDLADDDEENHFFDNYG
                     HWKEEYYPSSWQEEAVYSKEFWILFEVCLSNLPAKQARVFMMREYLEATSDEICRVVN
                     "
BASE COUNT          185 a           68 c           91 g          167 t
ORIGIN      
        1 ttctcacaaa aaattttctc aatataacac tatgattcat aacaagattt ctttagatat
       61 ttcatcggaa cagcttgccg atattcgtca gcaaatgttg aaatttgctc agttacaatt
      121 acaagatatt gctcttgctg aagatatggt tcaagaagca ttcgttaatg caataaaaaa
      181 tattgataat ttccagcgta aagcagcatt gaaaacctgg atatttgcta ttctaaaaaa
      241 taaaattatt gattatttac gtaaaaaaga tcgtatagta ctagaaagtg atctagctga
      301 tgatgacgaa gagaatcact tttttgataa ttatgggcat tggaaagaag aatattatcc
      361 ttctagctgg caggaagaag ctgtttattc caaagaattt tggatattat ttgaagtttg
      421 cttaagcaat ttacctgcaa aacaagctag ggtatttatg atgagggaat atttagaggc
      481 aacctcagat gaaatttgtc gagtagtaaa t
//
LOCUS       DANLUQ010000297          495 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.299, whole genome shotgun sequence.
ACCESSION   DANLUQ010000297 DANLUQ010000000
VERSION     DANLUQ010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.299"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..>495
                     /locus_tag="PXB27_002598"
     CDS             <1..>495
                     /locus_tag="PXB27_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlwI family type II restriction endonuclease"
                     /protein_id="HDL5347325.1"
                     /translation="LLKTSIDVSGSIVRPFIAVVKCLTELEFLSYDEFTYFVPLIRDD
                     ESAKQIISDIKLYREGKILPEEIIYKRLMQMENYKLAQEEFIASDVDENLICLVGMNR
                     KSRNYDKPYYKLYENLKKVFLDGENIYESLLNSAKNINQKPGILWRSLLFKTTNIGVV
                     RKNGK"
BASE COUNT          188 a           57 c           99 g          151 t
ORIGIN      
        1 cttcttaaaa cgagtataga tgtaagtggt agtatcgtta gaccgtttat tgcggttgta
       61 aagtgcctga cggaacttga gtttttaagt tatgacgagt ttacttattt tgttccgcta
      121 attagagatg acgaaagtgc aaaacaaatc atatcggata ttaaattata tcgagaaggt
      181 aaaattttac cggaagaaat aatttataag aggcttatgc aaatggaaaa ctacaaactt
      241 gcacaagaag aatttatagc ctctgatgtt gatgaaaatc taatatgttt agtgggaatg
      301 aatcgtaaaa gcagaaacta tgacaagccg tactataaat tgtatgagaa tttaaagaag
      361 gtatttcttg atggcgaaaa catttatgaa tcgctattaa attctgcaaa gaatataaat
      421 caaaaacctg gaatattgtg gagaagttta ctctttaaaa caaccaatat tggtgttgta
      481 agaaaaaatg ggaag
//
LOCUS       DANLUQ010000298          490 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.300, whole genome shotgun sequence.
ACCESSION   DANLUQ010000298 DANLUQ010000000
VERSION     DANLUQ010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.300"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..341)
                     /locus_tag="PXB27_002599"
     CDS             complement(<1..341)
                     /locus_tag="PXB27_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_046701250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL5347326.1"
                     /translation="MKITHCKLKKSLQKKLLEFFVAEVTARTAADLLGIQPNTAALFY
                     HKIRLVIEHHLALEAHEIFEGKIEVDESYFGGHRKGKRGRGAAGKVAVFGLLKRQGKV
                     FTKYQIRNANAG"
BASE COUNT          137 a          113 c           77 g          163 t
ORIGIN      
        1 ccggcattag cgtttctgat ttggtatttt gtaaacacct ttccttgacg cttcaacaaa
       61 ccgaatacag cgactttccc agccgcacct cgccctcgtt ttcctttgcg atgaccacca
      121 aaatagcttt catctacctc aatttttccc tcaaaaattt catgagcttc aagggctaaa
      181 tggtgttcaa tgaccagcct aattttgtgg taaaacaggg ctgccgtatt gggttgaata
      241 cctaataaat cagcagcggt tctagcggtc acttcagcaa caaaaaattc aagaagcttt
      301 ttctgcaaag atttctttaa tttacaatgg gttatcttca tttttgtagt atagcagtga
      361 tgctaatcta ctacagcccc taatcttttt tatgagccct tttcccttta tgcattacct
      421 taattgcatc ctcagaaatt aaatcacagc gaggtaatat ttcatttatt gacttccaag
      481 aaagtaaatc
//
LOCUS       DANLUQ010000299          489 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.301, whole genome shotgun sequence.
ACCESSION   DANLUQ010000299 DANLUQ010000000
VERSION     DANLUQ010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.301"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..354
                     /locus_tag="PXB27_002600"
     CDS             <1..354
                     /locus_tag="PXB27_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006247935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347327.1"
                     /translation="NIENDKINFNHQQNKYDSIINKAKNQNSQENNLTEINSNIQILA
                     EKHQLTIESLQWNLEQGKSIELSIIGNSRSLFDFIHSVKQIPYLKYNAISLIKSRQDR
                     KVEMNTTLVILANKE"
BASE COUNT          205 a           76 c           69 g          139 t
ORIGIN      
        1 aatatagaaa atgacaagat taactttaat catcaacaaa acaaatatga ctcaattatc
       61 aataaagcta aaaatcagaa cagccaagag aataatttaa ccgaaataaa cagcaacata
      121 caaatattag ctgaaaaaca ccaactaacc attgaaagtt tacaatggaa tttagaacaa
      181 ggtaaaagta ttgaattgag tattatcggc aatagccgat ctttatttga ttttatccat
      241 tcagttaagc aaatacctta tttgaaatac aatgcgatta gcctcatcaa atctcggcaa
      301 gacagaaaag ttgaaatgaa tactacctta gtcatattag ctaataagga ataacaatgc
      361 gtaattggtt tttatttatt actttattta tcagtttttc tgtaaaagca aatgatcctt
      421 tctttggaga aaaacaggaa actcaagcct caggcaacat cgctgaaagc aatgtggaat
      481 ttgcaaaaa
//
LOCUS       DANLUQ010000300          471 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.302, whole genome shotgun sequence.
ACCESSION   DANLUQ010000300 DANLUQ010000000
VERSION     DANLUQ010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.302"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            62..>471
                     /locus_tag="PXB27_002601"
     CDS             62..>471
                     /locus_tag="PXB27_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347328.1"
                     /translation="MELGKTTETDVKEMYNLEPEGVNKYSKGNQYFIDPTQLDLEDLK
                     STLIIFDEKGVLVFVGSEFNSISSIEDSKNRYKKFDYLYKILASKYKLVKKERPFVGD
                     QYAKFKDGNSEIELSEPHMGGFKINLTYAKKEFL"
BASE COUNT          184 a           63 c           78 g          146 t
ORIGIN      
        1 taaagctaca ttatttgcaa ctattagtac ttctgtatta gctgaaccaa ccgtttttaa
       61 aatggaacta ggtaaaacaa cagaaacaga tgtaaaagaa atgtataacc ttgaaccgga
      121 gggtgttaat aaatacagca agggtaatca gtattttatt gatccaacgc aattagattt
      181 agaagatctt aaatcaacgc ttattatctt tgatgaaaaa ggggtattag tttttgttgg
      241 ttccgagttt aattctatat cttctatcga ggattcaaaa aaccggtata aaaaattcga
      301 ttatttgtac aaaatactag caagcaaata taaacttgta aaaaaagaac gtccatttgt
      361 aggcgatcaa tatgctaaat ttaaagacgg taattcagaa atcgaattat cagaaccaca
      421 tatgggtggt tttaaaatta acttgactta tgcaaaaaaa gaattcttag a
//
LOCUS       DANLUQ010000301          469 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.303, whole genome shotgun sequence.
ACCESSION   DANLUQ010000301 DANLUQ010000000
VERSION     DANLUQ010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.303"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(116..191)
                     /locus_tag="PXB27_002602"
     tRNA            complement(116..191)
                     /locus_tag="PXB27_002602"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(156..158),aa:Thr,seq:ggt)
     gene            complement(195..269)
                     /locus_tag="PXB27_002603"
     tRNA            complement(195..269)
                     /locus_tag="PXB27_002603"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(234..236),aa:Gly,seq:tcc)
     gene            complement(313..397)
                     /locus_tag="PXB27_002604"
     tRNA            complement(313..397)
                     /locus_tag="PXB27_002604"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(361..363),aa:Tyr,seq:gta)
BASE COUNT          145 a          108 c          108 g          108 t
ORIGIN      
        1 tttctaaaag cctcttaaac aaaaacggtt acaatggaac aaaagaaccc attaaccata
       61 atgtagaaat taataccaat aaaaatttgc gtgagaaaga tagaattcag agaagtggtg
      121 ctgataggcg gatttgaacc gccgacctca cccttaccaa gggtgcgctc taccaactga
      181 gctatatcag cgcttggagc gggcagcggg aatcgaaccc gcatcattag cttggaaggc
      241 taaggtaata gccattatac gatgcccgct gttctaaatt cgtctgacct acgcgattca
      301 aaatatcaga gatggtggag ggagaaggat tcgaaccttc gaaggctgag ccaacagatt
      361 tacagtctgc cccctttggc cgctcgggaa cccctccacg ataattgaat acttatttac
      421 gcctgagtta atggtgccga ctaccggaat cgaactggtg acctactga
//
LOCUS       DANLUQ010000302          465 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.304, whole genome shotgun sequence.
ACCESSION   DANLUQ010000302 DANLUQ010000000
VERSION     DANLUQ010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.304"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
BASE COUNT          161 a           59 c           78 g          167 t
ORIGIN      
        1 aagcctgatt tataaggctg gattttttga tgtattaaac atcacaaata atctatgtat
       61 aaaactcagt caatttggtt tttatttcca aaatggcaat ttgagccaaa aagatgatga
      121 gcttttgagt ttatatagag aattctataa acataacgag tttatcactc atctcaagtc
      181 ccatagtctt tatggatgta ttgagcaacc tacttcggca ttttttcatt attgtactaa
      241 tgaaaacttt agaaatgcgt ttaatgaaga gagcctctat gattttcaaa tgtggaatgt
      301 gtttagatag gagtttttta tgtcatctca aaatagtttc ttaacaaaat attcaaaaag
      361 taattctcat agtcaggatt ctcaaaaaat aaatgagtca ttagaatggc tgaatgaaaa
      421 aaattatggt gaattatata gttcctttct gattgcagtg ggagt
//
LOCUS       DANLUQ010000303          461 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.305, whole genome shotgun sequence.
ACCESSION   DANLUQ010000303 DANLUQ010000000
VERSION     DANLUQ010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.305"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..183
                     /locus_tag="PXB27_002605"
     CDS             <1..183
                     /locus_tag="PXB27_002605"
                     /inference="COORDINATES: protein motif:HMM:NF019619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347329.1"
                     /translation="QEIAQFTGCHIFSSLYIAFNTQRSFKSHWDSRDIFAVQMQGKKR
                     WIIHTPTFKKTIVYAS"
     gene            173..>461
                     /locus_tag="PXB27_002606"
     CDS             173..>461
                     /locus_tag="PXB27_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HDL5347330.1"
                     /translation="MHRSKDMPEYAPNLDDVYMDIVLEAGDVLYLPRGWWHDPIPVGE
                     ETVHLAVGIFPAYTHNYLTWVSQNMVEKEIARASLSHYESDKELIAQLAEQT"
BASE COUNT          140 a           73 c           92 g          156 t
ORIGIN      
        1 caagagatag ctcaatttac aggctgtcat attttttcaa gtttatatat tgcgtttaat
       61 actcaaagat cttttaaaag ccattgggat agtcgtgata tttttgcagt tcaaatgcag
      121 ggtaaaaagc gttggattat tcacactcca acatttaaaa aaaccattgt ttatgcatcg
      181 tagtaaagat atgcctgaat atgctcctaa tcttgatgat gtatatatgg atattgtatt
      241 agaagctgga gatgttcttt atttacctcg tggctggtgg cacgatccta ttcctgttgg
      301 agaagaaact gtgcatttag ctgtaggcat ttttcctgct tatactcata actatctgac
      361 ttgggtatct caaaatatgg ttgagaaaga aattgcccga gcttctttat cgcattatga
      421 gtcagataaa gagttgatag cacagttggc agaacaaaca g
//
LOCUS       DANLUQ010000304          455 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.306, whole genome shotgun sequence.
ACCESSION   DANLUQ010000304 DANLUQ010000000
VERSION     DANLUQ010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.306"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            14..>455
                     /locus_tag="PXB27_002607"
     CDS             14..>455
                     /locus_tag="PXB27_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012263032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HDL5347331.1"
                     /translation="MMNQSYFNLLGNITWLWMNSSLHKEWSCELLARNVIPAIENEQY
                     MLLIDNGIPIAYCSWADLNLETEVKYIKDINSLTPEEWQSGDRRWIIDWVAPFGHSQL
                     LYKKMCQKYPDMIVRSIRFYPKQKELGKIAYFKGGKLDKKTAKKR"
BASE COUNT          163 a           75 c           85 g          132 t
ORIGIN      
        1 ttatcgagtg caaattatga atcaatctta ttttaactta ctaggaaaca ttacttggct
       61 atggatgaac tcctccctcc acaaagaatg gagctgtgaa ctactagcac gcaatgtgat
      121 tcctgcaatt gaaaatgaac aatatatgct acttatagat aacggtattc cgatcgctta
      181 ttgtagttgg gcagatttaa accttgagac tgaggtgaaa tatattaagg atattaattc
      241 gttaacacca gaagaatggc agtctggtga cagacgctgg attattgatt gggtagcacc
      301 attcggacat tctcaattac tttataaaaa aatgtgtcag aaataccctg atatgatcgt
      361 cagatctata cgcttttatc caaagcagaa agaattaggc aaaattgcct actttaaagg
      421 aggtaaatta gataaaaaaa cagcaaaaaa acgtt
//
LOCUS       DANLUQ010000305          453 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.307, whole genome shotgun sequence.
ACCESSION   DANLUQ010000305 DANLUQ010000000
VERSION     DANLUQ010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.307"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..>453)
                     /locus_tag="PXB27_002608"
     CDS             complement(<1..>453)
                     /locus_tag="PXB27_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009875102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HDL5347332.1"
                     /translation="CGREFMNSLEESSLEEVKQAIKSEPFLANFFDVGEKYVRTKDGR
                     ISYVFSGLRHNLDSIKSKARILIAWIDEAETVSETAWRKLIPTVRENNSEIWVTWNPE
                     NRGSATDTRFRQNPPENSCIIEMSYQDNPWFPDVLEQERLNDKQRLDDA"
BASE COUNT          120 a          111 c           82 g          140 t
ORIGIN      
        1 gcatcatcaa gccgctgttt gtcatttagc cgctcttgct cgagcacatc aggaaaccaa
       61 ggattatctt ggtaactcat ttcaattatg cagctgtttt caggtggatt ttgcctgaag
      121 cgagtatctg ttgcgctccc tctattttcc gggttccaag ttacccatat ttcggaatta
      181 ttctcacgca cggttggaat taatttccgc caagcagttt cgcttactgt ttctgcttca
      241 tctatccacg caatcaaaat acgggcttta gatttgatgc tatctaagtt atgccgaagt
      301 ccggaaaaca cataagaaat ccgcccatct ttagtgcgaa catacttctc gccgacatca
      361 aaaaaatttg ctaaaaaagg ctcagacttg atcgcctgtt taacttcttc aagagagctt
      421 tcttccaatg agttcataaa ctcacgaccg caa
//
LOCUS       DANLUQ010000306          447 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.308, whole genome shotgun sequence.
ACCESSION   DANLUQ010000306 DANLUQ010000000
VERSION     DANLUQ010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.308"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            24..>447
                     /locus_tag="PXB27_002609"
     CDS             24..>447
                     /locus_tag="PXB27_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HDL5347333.1"
                     /translation="MLSKLQRFTLMIKSTIIKHPLEILFVTYVTILLINNVSDYELIK
                     RFQNLIALFPICFILLYLIRDTKAYWLFAPIPIAIVFFFGQINLTLLEDSRYWATIFC
                     VFLCLISQYWYKNNHYFVAGTTNKLVNIVFATVLSGIIF"
BASE COUNT          128 a           82 c           64 g          173 t
ORIGIN      
        1 ttttcatagg caggagatct attatgctaa gtaagctaca acgctttacc ttaatgataa
       61 aaagcaccat cattaagcat ccactggaaa ttttattcgt tacttacgtc acgattttac
      121 ttatcaataa tgtgagcgac tacgaattaa tcaaacgctt tcaaaatcta attgctcttt
      181 ttcctatttg ctttattttg ctttacctga tacgcgatac caaagcttat tggctattcg
      241 ctccgattcc gatagcaatc gtctttttct ttggtcaaat taatttaaca ttattggaag
      301 attctcgtta ttgggctacc atattttgtg tattcctttg tttgatcagt caatattggt
      361 acaaaaacaa ccattatttt gtagccggaa caactaataa attagttaac attgtctttg
      421 ctacagtttt atctggcatt atttttg
//
LOCUS       DANLUQ010000307          438 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.309, whole genome shotgun sequence.
ACCESSION   DANLUQ010000307 DANLUQ010000000
VERSION     DANLUQ010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.309"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            36..>438
                     /locus_tag="PXB27_002610"
     CDS             36..>438
                     /locus_tag="PXB27_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HDL5347334.1"
                     /translation="MLEKIEKLIDEINKLHLAFSKDYFETGKIEKVNLKHTFAKVPTP
                     HILAYRLNLHESINDYLYRADLEGISYFYRVKTSESILDKIERFKQRSEGYPVNSILN
                     DIFGARIIVSSEVIQQVMESLDYWQEKYGLKN"
BASE COUNT          157 a           58 c           81 g          142 t
ORIGIN      
        1 tttggggcaa gataataaat tagagagtga atagcatgct tgagaaaata gaaaaactca
       61 ttgatgaaat caacaagttg cacttagctt tttcgaaaga ctactttgaa acaggtaaga
      121 ttgaaaaagt gaatcttaag catacttttg ccaaagtgcc tacgccacat attttagctt
      181 atcgtcttaa tttacacgaa tctattaatg attatttata tcgtgcagat ttagagggaa
      241 tttcttattt ttaccgagta aaaacctcag aatcgatttt agataaaatc gagcgtttta
      301 aacagcgtag tgaaggttat ccagtaaata gcattctcaa cgatattttt ggagctcgta
      361 ttattgtttc tagtgaagtg attcagcaag taatggagtc attagattat tggcaagaaa
      421 aatatggact aaaaaatt
//
LOCUS       DANLUQ010000308          424 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.310, whole genome shotgun sequence.
ACCESSION   DANLUQ010000308 DANLUQ010000000
VERSION     DANLUQ010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.310"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            complement(<1..>424)
                     /locus_tag="PXB27_002611"
     CDS             complement(<1..>424)
                     /locus_tag="PXB27_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL5347335.1"
                     /translation="RMDADDISLPTRLSEQILYLEENHLDLCGCNALLFSEHGNLGLT
                     NKLNSHEALEKLMVAGDIGIIHPTFLARRKVFVESGGYNPLALHAEDMEFIAHILSMG
                     FRLGNNPKVLFKYRFSTNSVTRRNAFHLYTTISNINKA"
BASE COUNT          148 a           80 c           55 g          141 t
ORIGIN      
        1 tatgctttat ttatattact tattgtggta tataaatgaa aagcatttct tctagtaacg
       61 ctatttgtag aaaacctata tttgaataat accttaggat tattccctaa tctaaatccc
      121 attgataaaa tatgggcaat aaattccata tcttccgcat gtaaagctaa gggattatac
      181 cctccagatt caacaaatac ttttctcctt gctaaaaaag tagggtgaat tattccaata
      241 tcaccagcca ccataagctt ttctaaagcc tcatgactat tcaacttatt tgtaagacca
      301 aggttgccat gttcagaaaa aagcaaagca ttacaaccgc ataagtctaa atggttttct
      361 tctaaatata aaatttgctc tgacaatcta gtaggtaaag atatatcatc agcatccatt
      421 ctcg
//
LOCUS       DANLUQ010000309          408 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.311, whole genome shotgun sequence.
ACCESSION   DANLUQ010000309 DANLUQ010000000
VERSION     DANLUQ010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.311"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            119..403
                     /gene="raiA"
                     /locus_tag="PXB27_002612"
     CDS             119..403
                     /gene="raiA"
                     /locus_tag="PXB27_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005823326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDL5347336.1"
                     /translation="MSLNISSKQMDITPAIRTHIEERLAKLEKYHTQLINPHFMLIKQ
                     PNSFQVEATIGTPLGDLVAKAEHEDLYTAIFDVKKKLEGQLIKLKEKNEH"
BASE COUNT          153 a           63 c           66 g          126 t
ORIGIN      
        1 gtagatcaaa tttttggaaa cttatttcat ttttcacttg aatttttgga gtgatgagca
       61 tattttggtt attagaatat caacggcaaa agccttaact aaacaagagg aaaatgttat
      121 gtcattaaat atttcaagca aacaaatgga cattacccct gcaatccgta ctcatattga
      181 ggagcgttta gctaagttag aaaaatacca cacccaattg ataaacccac attttatgct
      241 gataaaacaa cctaactcat tccaagttga agcaacaatt ggcacccctt taggtgattt
      301 agtcgctaaa gctgaacatg aagatttata tactgcaatt tttgatgtta agaagaaatt
      361 agaaggtcag ttaattaaac taaaagagaa aaatgaacat tgatgttt
//
LOCUS       DANLUQ010000310          377 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.312, whole genome shotgun sequence.
ACCESSION   DANLUQ010000310 DANLUQ010000000
VERSION     DANLUQ010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.312"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
BASE COUNT          120 a           65 c           64 g          128 t
ORIGIN      
        1 agagagttta ttattagaga ggatatcgcc cttctatact tcatacttat gggattatat
       61 cctctgtatt tcgtaaatat aaaccgtgaa tacgtccatt atgatgatca aagttattct
      121 ttataagatc gtaaaatgag ctatcattat gttcataatg cctaaatatg gcataggaaa
      181 ccaaatcagc taataaatta atcttgatga ctttgagtca agaaacagcg gagcttctga
      241 gaagttcctg agcttcccat aagtatgatc actatcctta aatgttcttg caagtgcctt
      301 aattgacttc tccgttgatg atttatcaaa gattgagctc ctttgagtat tgccttgata
      361 atgcaaatgc ccaagat
//
LOCUS       DANLUQ010000311          374 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.313, whole genome shotgun sequence.
ACCESSION   DANLUQ010000311 DANLUQ010000000
VERSION     DANLUQ010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.313"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            20..325
                     /locus_tag="PXB27_002613"
     CDS             20..325
                     /locus_tag="PXB27_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347337.1"
                     /translation="MSKLNKLLSRKTSRKIIPEQVNFDINLPIYIPLFLSDKTAKIQA
                     VWELEQENGERDAGAVKRNRIQFRYLPEGVHKLTLIVGTPLLSKGSKVYQCLIKVNK"
BASE COUNT          135 a           49 c           68 g          122 t
ORIGIN      
        1 ttttacaaag gaacattcta tgtctaagtt aaacaaatta ttatcgagaa aaacatcgcg
       61 taaaattatt cctgagcaag ttaattttga cattaacttg cctatctata tcccgctgtt
      121 tttatcagat aagacagcga aaattcaagc agtatgggag ttagaacaag aaaatggtga
      181 gcgtgatgct ggcgctgtaa agcgaaatcg tattcagttt agatacttgc cagaaggggt
      241 acataaatta acattaattg ttggtacgcc tttattatca aaaggaagta aggtttatca
      301 atgcctaatt aaagttaata aataatatta actttatgtt gtgatcgtat ttatgtgtat
      361 attgtaaaac agaa
//
LOCUS       DANLUQ010000312          359 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.314, whole genome shotgun sequence.
ACCESSION   DANLUQ010000312 DANLUQ010000000
VERSION     DANLUQ010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.314"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..>359
                     /locus_tag="PXB27_002614"
     CDS             <1..>359
                     /locus_tag="PXB27_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL5347338.1"
                     /translation="GSMSGTGIPRITLTKLESYLVPVPPLLEQQRIVQKIEELLPLVE
                     RYEQTEQQLTKLNNTFPEQLKKSVLHAAIQGKLTEQDPNDELASCLIERIKAEKNRLI
                     AEKKLKKPKSVSEIVMR"
BASE COUNT          133 a           75 c           70 g           81 t
ORIGIN      
        1 ggaagtatgt ctggaacagg tattccaaga ataacattaa ctaaattaga atcttattta
       61 gtcccagttc cgccactcct agaacaacag cgcattgtgc aaaaaatcga ggaattatta
      121 ccgcttgttg aacgctacga gcaaaccgaa cagcaactca ctaaactcaa caatacattc
      181 cccgaacagc tgaaaaaatc ggtgttacac gcagctattc agggtaaatt aaccgagcaa
      241 gatccgaatg atgaactggc atcttgcctg attgagcgga taaaagcgga gaaaaatcgt
      301 cttattgccg agaaaaaact caaaaaacca aaaagtgttt cagagattgt aatgcgtga
//
LOCUS       DANLUQ010000313          340 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.315, whole genome shotgun sequence.
ACCESSION   DANLUQ010000313 DANLUQ010000000
VERSION     DANLUQ010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.315"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..>340
                     /locus_tag="PXB27_002615"
     CDS             <1..>340
                     /locus_tag="PXB27_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347339.1"
                     /translation="LVLTGQAGSGKSHLLGDFVQKRQNSGKYSLLLLGQDFTEKSNAW
                     NQILSKQLNLNITPEVFLSALDSIGEMQKERVILAIDALNEGEGKSLWNNQLNGLIEL
                     VSQYPNIGLVL"
BASE COUNT          116 a           47 c           69 g          108 t
ORIGIN      
        1 gcttgtttta actggtcaag ctggtagtgg aaaatctcat ttattgggtg attttgtcca
       61 aaaaagacaa aattctggaa agtattcttt gttgttattg gggcaggatt ttacagaaaa
      121 atccaatgct tggaatcaaa tcttatccaa acaattaaat cttaatatca cacccgaagt
      181 atttttatcc gcattagata gtatcggaga aatgcaaaaa gaacgggtta ttttagccat
      241 agacgcctta aatgagggtg aagggaaatc attatggaat aaccaactta atggattaat
      301 tgagttggta agtcaatatc caaatattgg actggttctg
//
LOCUS       DANLUQ010000314          338 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.316, whole genome shotgun sequence.
ACCESSION   DANLUQ010000314 DANLUQ010000000
VERSION     DANLUQ010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.316"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..>338
                     /locus_tag="PXB27_002616"
     CDS             <1..>338
                     /locus_tag="PXB27_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006253310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL5347340.1"
                     /translation="AVDSLSIGARNLLIDSTYNQHINYNTKMSSITRTVYRGNMLIRL
                     GMLGGAGVAGIVQAPAAQVSNIRQGQEYTLSLNVQGTAGIQKTGLNYVFLMRADGANQ
                     RLATIPVIAS"
BASE COUNT          104 a           79 c           76 g           79 t
ORIGIN      
        1 ctgccgttga ttcactttct atcggtgcaa ggaacttact gatagacagt acttacaacc
       61 aacacatcaa ctacaataca aaaatgtcat caatcacacg cacagtctat cgcggcaata
      121 tgttgatacg actcggaatg cttggtggcg caggtgtggc cggtattgta caagcaccag
      181 ccgctcaagt aagtaatatc agacagggtc aagagtacac acttagcctg aatgtacagg
      241 gtacagccgg catacaaaaa acggggctca actatgtatt cttgatgcgt gctgatgggg
      301 ctaatcaacg gttagcaaca atacctgtta tagccagc
//
LOCUS       DANLUQ010000315          332 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.317, whole genome shotgun sequence.
ACCESSION   DANLUQ010000315 DANLUQ010000000
VERSION     DANLUQ010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.317"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..>332
                     /locus_tag="PXB27_002617"
     CDS             <1..>332
                     /locus_tag="PXB27_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011150324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HDL5347341.1"
                     /translation="LRKQGIFISAGGVRSIWLRHNLANFKQRLNALEKAVAEKGIILS
                     ENQVQALERKKEDDVACGEIETAHPGYLGSQDTFYVGNLKGVGRIYQQTFIDTYSKVA
                     FAKLYTMK"
BASE COUNT          102 a           57 c           79 g           94 t
ORIGIN      
        1 aactccgtaa acaaggtatt tttatttcag caggtggtgt gcgttctatt tggctacgcc
       61 ataatctcgc taactttaaa cagcgtttaa atgcgttaga aaaagcagtg gctgagaaag
      121 gcattattct gagtgaaaac caagttcaag ccttggagcg taaaaaagaa gacgatgtgg
      181 cttgtggcga aattgaaacg gctcatccgg gctatctagg ttcacaagat accttctatg
      241 tggggaatct taagggggtt ggacgcattt atcaacagac ctttattgat acttatagta
      301 aagtcgcatt tgcaaaactg tatacgatga ag
//
LOCUS       DANLUQ010000316          327 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.318, whole genome shotgun sequence.
ACCESSION   DANLUQ010000316 DANLUQ010000000
VERSION     DANLUQ010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.318"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..>327
                     /locus_tag="PXB27_002618"
     CDS             <1..>327
                     /locus_tag="PXB27_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine methyltransferase"
                     /protein_id="HDL5347342.1"
                     /translation="RDVKANSFVIYPYVKKDKKIELINISILKEKYPYGFAFLTKVKS
                     KLLQRNIWPPIMENDWYKYGRHQALENCDSAPKIIVGILSKGYKYSVDHEGVFISSGG
                     TAGYSLI"
BASE COUNT          125 a           45 c           56 g          101 t
ORIGIN      
        1 agagatgtca aagctaatag ttttgtaatt tacccttatg taaaaaaaga taaaaaaata
       61 gaattgatta atatctccat attaaaagag aaataccctt atggatttgc ttttctaaca
      121 aaagttaagt caaaattatt acaaagaaat atatggcccc cgataatgga aaacgattgg
      181 tacaaatacg gtcgccatca agctttagag aattgcgact ccgcccctaa aattattgtt
      241 ggcattctat caaaaggata taaatatagt gttgatcatg aaggggtatt tatatcatca
      301 ggtggaactg ctggatatag tttaatt
//
LOCUS       DANLUQ010000317          325 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.319, whole genome shotgun sequence.
ACCESSION   DANLUQ010000317 DANLUQ010000000
VERSION     DANLUQ010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.319"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..>325
                     /locus_tag="PXB27_002619"
     CDS             <1..>325
                     /locus_tag="PXB27_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL5347343.1"
                     /translation="AGQERCIADEVPFEIPQNWIWVRLENYSLNHDRRRKPVSVAQRS
                     QQNKLYDYYGATGAIDKVASYIFDGKFILIGEDGGNFFTKKDVAFIVEGKFWANNHVH
                     VLSVDF"
BASE COUNT          108 a           37 c           75 g          105 t
ORIGIN      
        1 gcaggtcagg aacggtgtat cgccgatgaa gtgccatttg agattcctca aaattggatt
       61 tgggtgaggt tggaaaatta ttctttaaat catgatagaa gaagaaaacc agtatcagtt
      121 gcacagcgta gccaacaaaa taaattgtat gactattatg gagcgactgg agcaatagat
      181 aaagtggcaa gctatatttt tgatggtaag tttattttaa ttggagaaga tggaggtaat
      241 ttcttcacta aaaaagacgt tgcttttatt gttgaaggta aattttgggc aaataatcat
      301 gttcatgtat tatcagttga tttta
//
LOCUS       DANLUQ010000318          318 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.320, whole genome shotgun sequence.
ACCESSION   DANLUQ010000318 DANLUQ010000000
VERSION     DANLUQ010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.320"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..>318
                     /locus_tag="PXB27_002620"
     CDS             <1..>318
                     /locus_tag="PXB27_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015484059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HDL5347344.1"
                     /translation="AGQERCIADEVPFEIPQNWTWVRLGEVGDWSSGATPNRHNPEYY
                     NGTIPWLKTGDLNNGIISSIPEFITDKALQECSVRLNPVGSVLIAMYGATIGKLGILK
                     IAAT"
BASE COUNT          103 a           51 c           79 g           85 t
ORIGIN      
        1 gcaggtcagg aacggtgtat cgccgatgaa gtgccatttg agattcctca aaattggact
       61 tgggtgaggt tgggggaagt aggtgattgg agttcaggtg caactccaaa ccgacacaat
      121 cctgaatact ataatggtac gatcccgtgg ctaaaaacag gagacttaaa caacggaata
      181 atttcatcta tacctgaatt tattacagat aaagcattgc aggaatgttc tgtaagatta
      241 aatcctgttg gttcagtttt gatagcaatg tatggagcga caattggtaa gcttgggata
      301 ttaaaaatag cagcaaca
//
LOCUS       DANLUQ010000319          306 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.denovo.322, whole genome shotgun sequence.
ACCESSION   DANLUQ010000319 DANLUQ010000000
VERSION     DANLUQ010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.denovo.322"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..>306
                     /locus_tag="PXB27_002621"
     CDS             <1..>306
                     /locus_tag="PXB27_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HDL5347345.1"
                     /translation="SRQYCDAYHLLENVARWEKPKVYGVARKMDRTSLKSDYCMIAAT
                     KAFEELIENADAKYILLSYNNMSDKGNDRSNAKISDEDIMKILSKKGKVIVFESDYK"
BASE COUNT          119 a           32 c           64 g           91 t
ORIGIN      
        1 ttcaagacaa tattgtgatg catatcattt acttgaaaat gtggcacgat gggaaaaacc
       61 taaagtttat ggtgttgcaa gaaagatgga tagaacttcg cttaaaagtg attattgtat
      121 gattgcagct acaaaagcat ttgaggagct tattgaaaat gctgatgcaa aatatatttt
      181 gctttcatat aacaatatgt cagacaaggg aaatgataga tccaatgcga aaatatcgga
      241 tgaagatatt atgaaaattc ttagcaaaaa gggaaaagta attgtatttg aatcggatta
      301 taagag
//
LOCUS       DANLUQ010000320         8895 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.guided.105, whole genome shotgun sequence.
ACCESSION   DANLUQ010000320 DANLUQ010000000
VERSION     DANLUQ010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 8895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8895
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.guided.105"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            <1..650
                     /locus_tag="PXB27_002622"
     CDS             <1..650
                     /locus_tag="PXB27_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="HDL5347346.1"
                     /translation="KAAKEKAEAERLAAEKEVDPIAIIKAKGIDDTNYGLKLGNSKET
                     IEGKVTVRTQSYLYNQPYSVVTAQAVQRNGWENGNYIDKVDGTVTVKGLKTEKLPTEG
                     TATYSGKAFNHDGAKGFNGGDLTYNVNFSKRTGSGRVEGEFGNLIVLEEGTINNTQIS
                     STVHGNNPLNLSGKYTVEFFGKNAEEIAGEIQTKANLTGEVSNFGLAGTRGDIQK"
     gene            846..2255
                     /locus_tag="PXB27_002623"
     CDS             846..2255
                     /locus_tag="PXB27_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HDL5347347.1"
                     /translation="MKKYPLLFSLFSPVVWAEKVIEHNTDPISFERQPQAVKFEQNVV
                     KNSQKPTAYQPAYSVNQNVEQQINQAILSQDWATLEPLLSRYPQTENFDPLLWHYAQA
                     ALAYAKKDHQTAVFHYRQLLAKPPSLVYPRFDLALILAEDQQFRAAEQEFKAIQAHLP
                     PELAKIARNTQVQIAENEAWQPDFYLQYTQTDNVNNASSSPIVNVNGRIFRKNADSLP
                     QSAKGVRYGLGLSKLHNVKGNHFVGLTLNYSGIYYWDNRDYREQSVSVSPNYSYRTVA
                     YRLTFSPFIEQNWLGSSRYNVQFGATLNGLRYLNSQWLVSGSFTHLQKRYFEELTASR
                     YNSYQHQLSGGVQWQAVKNGRFFANLDGVREIAREKAQSSNKWLARVGAVFQGERWAT
                     QASIGFGKRYFADKHYLFGYKRLDTEYQANLAVWNRTWHWQGIVPKLNFRYQKIDSNI
                     ADFYSRQNKEVFFTLEKLF"
     gene            2401..2874
                     /gene="gpt"
                     /locus_tag="PXB27_002624"
     CDS             2401..2874
                     /gene="gpt"
                     /locus_tag="PXB27_002624"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDL5347348.1"
                     /translation="MSEKYTNEKYVVTWDMFHMHARKLAENLLPATQWKGIIAVSRGG
                     LFPAAVLARELGIRHVETVCIASYDHDSQGELNVLHAADTDGEGFIVVDDLVDTGNTA
                     KEIRRMYPKARFVTVFAKPHGAPLVDDYVIDIPQETWIEQPWDLGITFVQPLARR"
     gene            2987..3610
                     /gene="gmk"
                     /locus_tag="PXB27_002625"
     CDS             2987..3610
                     /gene="gmk"
                     /locus_tag="PXB27_002625"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDL5347349.1"
                     /translation="MSLGNLYIISAPSGAGKSSLINALLADLPRSEVQLSISHTTRQP
                     RPAEQDGVHYYFTSHDEFKQLIEQGYFLEWAEVFGNYYGTSLPMIERSLEQGIDVFLD
                     IDWQGARQIREKVPNVKTVFILPPSKTELEKRLIGRGQDSAETIAKRMAEAVSEMNHY
                     NEFDYVIINDDFQQALAELKAILIAERLSLGSQQIRHQHLITDLLNN"
     gene            3700..5097
                     /locus_tag="PXB27_002626"
     CDS             3700..5097
                     /locus_tag="PXB27_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005596137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HDL5347350.1"
                     /translation="MKLSKLAFSVALALTVTACTTGNMSNDGTLQQAQQTYQSYDEIT
                     KQYQINEQWWQGYTDAELNRVVDLAIKNNLDLAKSGIAVNKALYNANLVGANLVPTFS
                     GSGSVSAAKGLGSASSNNVSTGVSTTAAQAAFNLSYTVDLWRRLADTASAAEWTHKAT
                     IEDLKATRLSIINAVVTTYYQLAYYRDAIRITEESIRHYEQISRILTNKMNAGAIDSL
                     AVDQAQQAVLNARNSVIDLKTNQKIAEQTLRNLLNVQPNTPLNIRYPNIMSVKLQGVD
                     LNVPVSAIANRPDVVGSLQRLQSAFKTLTATEKSWFPTLTIGGSITGSATNIGNVADN
                     PVGNGLIKLDLPFLDWNRVKNNVKISETDYVTARLNYEQKITSALNEIDRYYYSYTQS
                     RSSLKILQDTNAKNKRISSYYKNRYEQGVSEFREWITALNTELNSQLAILNAKYAILT
                     NENAVYQAMAGKYRR"
     gene            complement(5210..6055)
                     /locus_tag="PXB27_002627"
     CDS             complement(5210..6055)
                     /locus_tag="PXB27_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL5347351.1"
                     /translation="MTFVKKTALSLIATALFATSSFAAEQSQYQHIRNATAKIEYAGK
                     TFLVDPYLAEKDRYLPPNAKKRNPTLDMTVSIDEVLNGVDAVIVTHTHHAHWDDVAQQ
                     RVPKNLPIFVQNAGDARLIRSQGFTDVRVVGKDTPFEGVTLTRTRGQHGTDAMYANPK
                     IAEMVGDAMDVVMKAEGKPTVYLVGDTIWNHEIEHSFNTYKPDFVVLNAGCAKLPNFE
                     GSPIMCTEDVSKAHKIAPQAKILTVHMDALPQTSISSDQMRDYVQKEGLNRVTVPKNG
                     DVIKF"
     gene            complement(6196..>8895)
                     /gene="ttrA"
                     /locus_tag="PXB27_002628"
     CDS             complement(6196..>8895)
                     /gene="ttrA"
                     /locus_tag="PXB27_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006251007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetrathionate reductase subunit TtrA"
                     /protein_id="HDL5347352.1"
                     /translation="GIENRSTACARGAAFLEGVNSPYRITQPLKRVGKRGEGKWQTIS
                     FEQLVEEIINGGNLFGEGEVEGLKTIRNLVDPINPKQPDLGMKANQLLVTFAGPEGRQ
                     PILQRFAQKSFGTINFSNHGAYCGAAYRAGSGAFMKDFDNQQHAKPDWDHAEFILFIG
                     TSPAQSGNPFKRQARQLAKRRPDDNFSYVVVSPRLEMTSSLATQNNHWVPIKPEGDLA
                     LVMGMLRWIIENERFNADYLSRPSLAAMENAGHVSYANASHLIICEPNHPKFGQALRI
                     SDLQDVQLDPEEKVEENLIVVKDQASGELISAKDCLQAVIFVEQIAKLLDGSEVLVKS
                     SMQLLKEATQEKTLAEYSDICGVPVQTIENLAKKFTSHGHKANAITHGGTMHSTGFYT
                     SWAILLLNAMVGNLNKKGGMAVAAGKFKDFGDGPRYNLANFPNEVKPKGTNLARSKKA
                     YQNSSEFKQKVTKGENPYPAQGAWYPFTGGQTTEMFTSMVQGYPYFAKAWINHMANPV
                     YGLTAMNPIALEKLKDPKILPLFVSIDAFMNETTALTDYIVPDTHNFESWGFSTAWGG
                     VPQRTSTARHPIVPSKNAKTAEGDTICMENFVIAVAKKMGLPGFGENAIADLEGNRYP
                     LHRTEDYFLRAAANIAFAGEKPAPDASEQDLLLTGVQRIMPKIEQTLKAEERLKVANV
                     YCKGGRFAPHESAWKEENMEFQWKNCLQIWNENVYKAQHHSNGEHYWGCPRYMSPRFA
                     DGSTLEQHYPQSEWGFKLISFKSNIMSSITAPLLRLLSIKPEGLVAMNRLDAEEKGIK
                     HGDVVKLSTPTAGLTVQIVVLDGIARGTIGIEHGYGHKQIGASSYTIDGVEIPANPMI
                     AKGINLNDLGIIDHTKAVKSPWVDWVCGSAVRNGIPAKVEKLA"
BASE COUNT         2406 a         1945 c         2053 g         2491 t
ORIGIN      
        1 aaaaagcggc gaaagagaaa gcggaagcgg agcgtttagc agcagaaaaa gaagtagatc
       61 ctatagctat tattaaagca aaaggtattg atgatacaaa ttacggctta aaacttggta
      121 atagtaaaga aactattgag ggtaaagtca cagtcagaac acagagttat ttatataatc
      181 agccatattc tgtagtaaca gcacaagcag ttcaaagaaa tggctgggaa aacggtaatt
      241 atattgataa agttgatgga acagttacag ttaaaggatt gaaaacagag aaattaccaa
      301 cagagggtac tgcaacatat tcaggaaaag catttaacca cgatggagct aaaggtttta
      361 atggtggtga tctaacctat aatgttaatt tctctaagcg taccggttca ggtagagttg
      421 agggagaatt tggaaactta atcgtattag aagaggggac gattaataat actcagattt
      481 cttcaacagt acatggcaat aatcctttaa acctttctgg aaaatataca gtcgaatttt
      541 tcggtaaaaa tgcagaggaa attgccggtg aaattcagac aaaagcaaat cttactgggg
      601 aagtgagtaa ttttggactt gccggcactc gtggcgatat ccaaaaataa ccttttctaa
      661 cgttcaatgc cgtttaacga aaaattaaac ggcatttgtc ttgatattgc ttgtagatac
      721 aataagacac aatagtttag atttcaattt acagcttcta ataaccctct cccacaaggg
      781 gagagagtga taaatcccgt agaggcgaac atataggtcc gcccctacca attttcttta
      841 tctttatgaa aaaatatccc cttttatttt cgctgttcag ccccgttgta tgggcagaaa
      901 aggtgattga acacaacact gacccgattt cctttgagcg tcagccgcaa gcggttaaat
      961 ttgagcaaaa tgttgtaaaa aattcacaaa aaccgaccgc ttatcagcct gcgtattcag
     1021 tcaatcagaa tgttgaacag caaattaacc aagcgattct cagccaagat tgggcaacgc
     1081 ttgaaccgct tttaagccgt tatccgcaaa ctgaaaattt tgacccgttg ttgtggcact
     1141 atgcgcaagc cgcacttgcc tatgcgaaaa aagatcatca aaccgcagtt tttcactatc
     1201 gccaactgtt agctaagccc ccgagtttgg tttaccctcg ttttgatttg gcgttgattt
     1261 tggcagaaga tcagcaattt cgtgcggcag agcaggaatt taaggctatt caagcccatt
     1321 taccgccgga acttgctaaa attgcccgca atactcaagt ccaaattgct gaaaatgagg
     1381 cgtggcagcc cgatttttat ctgcaatata cccaaacaga taacgtgaat aatgcctcat
     1441 cttcgccgat tgtgaatgta aatgggcgga ttttccgcaa aaatgcagac agtttgccac
     1501 aatcagcaaa aggcgtgcgt tatggcttag ggttgagtaa attgcacaac gtgaaaggca
     1561 atcattttgt cggtttaacg ctcaattata gcgggattta ctattgggat aaccgagatt
     1621 accgtgagca atccgtatcc gtttcgccga attatagcta ccgcaccgtc gcttatcggc
     1681 tgactttttc gccttttatt gagcaaaatt ggctggggag tagccgttat aatgttcaat
     1741 ttggggcaac actcaacggt ttgcgttacc ttaattcgca atggttggtg tcggggagtt
     1801 ttacccattt gcaaaaacgg tattttgagg agctaaccgc aagccgttat aacagctatc
     1861 agcaccaact cagtggcggt gtgcagtggc aagcggtcaa aaatgggcgg ttttttgcaa
     1921 atttagacgg cgtgcgtgaa attgcacggg aaaaagcaca aagctcgaat aagtggctgg
     1981 ctcgagtggg ggcggttttt caaggcgaac gctgggcaac gcaggcaagt atcggctttg
     2041 gcaagcgata ttttgccgac aagcactatt tgtttggcta taagcggtta gacacggaat
     2101 atcaagccaa tttagcggtt tggaaccgca cttggcattg gcagggcatt gtgccgaagc
     2161 tgaatttccg ttatcaaaaa attgacagca atattgctga tttttactct cgccaaaaca
     2221 aggaagtctt tttcacttta gaaaagctat tttagtgttg gttttttgtg ctaagcatac
     2281 gattgcgtag ctgccggcac aaaaaaataa atgaaatgat ggcgaaaaaa gcattttcgg
     2341 gttatcctag tgcaattttt tatatcgggg tagcccccaa ttttatatta ggttgtttct
     2401 atgagtgaaa aatacacaaa tgaaaaatat gtcgtgacgt gggatatgtt ccatatgcac
     2461 gctcgcaagt tggcagaaaa tttattacct gccacacagt ggaaagggat tattgcggtg
     2521 agccgtggcg gcttgtttcc ggcagccgtt ttagcacgtg agttgggtat tcgccacgta
     2581 gaaacagtgt gcattgccag ctacgatcac gatagccaag gtgagttaaa tgtgttacac
     2641 gcagccgata ccgatggcga aggctttatt gtggtagatg atttagtgga taccggcaac
     2701 acggcaaaag aaattcgcag aatgtacccg aaagctcgtt ttgtaaccgt gtttgcgaaa
     2761 ccgcacggtg caccgttggt tgatgattat gtgattgata ttccacaaga aacgtggatt
     2821 gagcagcctt gggatttagg cattaccttc gttcagcctt tggcacgtag ataattttgt
     2881 gtaatggaca atgaaagttg tccattattt tttatttatt cctatgaaat gtaggggcga
     2941 atcatcattc gccctattcc caaaatcaaa taggtatata caaataatga gtttaggcaa
     3001 tctttatatt atttctgccc caagtggagc aggaaaatct tccttaatta acgccttgtt
     3061 ggcggattta ccccgttcgg aagtacagct ttcgatttca cacactaccc gtcagcctcg
     3121 tccggcagaa caggacggcg tgcattatta tttcacttcg cacgatgagt ttaagcagct
     3181 gattgagcaa gggtattttt tagaatgggc ggaggtgttt ggcaattatt acggcacatc
     3241 attgccgatg attgaacgta gcctagagca gggcattgat gtctttttgg atattgactg
     3301 gcaaggggct agacaaattc gggaaaaagt gccgaatgtg aaaaccgttt ttattttgcc
     3361 accgtcaaaa acagaattgg aaaaacgttt aatcgggcgt gggcaagatt ccgctgaaac
     3421 cattgccaaa cgtatggcag aggcggtgtc ggaaatgaac cattacaatg agttcgatta
     3481 tgtgattatt aatgatgatt tccaacaggc attggcagag ctaaaagcga ttttaattgc
     3541 cgagcgtttg agcttaggtt cgcaacaaat tcgtcatcag catttgatta ctgatttgtt
     3601 aaataattaa atgaacagtt gttcatttaa ctggaaaggc aatctgtttt tgggtagaat
     3661 acgcccattt tcccctcttt attttcaaaa ggaaatgtaa tgaaactttc aaaattagca
     3721 ttttctgtgg cgttggctct aaccgtaacg gcttgtacca cagggaatat gagcaatgac
     3781 gggactttgc aacaagcaca gcaaacctat caaagctatg atgaaattac caaacaatat
     3841 caaattaacg agcaatggtg gcaaggctat acggatgcgg aattaaaccg tgtggttgat
     3901 ttagccatca aaaataacct tgatttagcc aaaagcggaa ttgcagtcaa taaagccttg
     3961 tataacgcta atttagtagg ggcaaattta gtgccaacct ttagtggttc aggttctgtt
     4021 tctgcggcta aaggtttagg ttcggcttct tcaaataatg tttcgaccgg tgtttcaaca
     4081 accgcagctc aagctgcatt taatttaagt tatactgtgg atttatggcg acgtttagcc
     4141 gataccgcca gtgcggcaga atggacgcat aaagccacca ttgaagactt aaaagctacc
     4201 cgtttatcta ttattaatgc ggttgtgact acttactacc agttagctta ctatcgtgat
     4261 gcgattcgta ttaccgaaga gagcattcgc cattatgagc aaatcagccg tattttaact
     4321 aacaaaatga atgccggtgc gattgatagt ttagcggttg atcaggctca acaagcggtc
     4381 ttaaacgcca gaaatagtgt aattgatctg aaaaccaacc aaaaaattgc cgagcaaaca
     4441 ttacgcaatt tgttaaatgt tcagccaaat actccgctta acattcgtta cccgaatatt
     4501 atgagcgtga aattgcaagg ggtagattta aatgtgccag tgtcagccat tgctaatcgc
     4561 cctgatgtag taggctcgtt acaacgctta caaagtgcat ttaaaacctt aaccgcaacc
     4621 gagaaaagct ggttcccaac cttaaccatc ggtggttcta ttaccggtag tgcaaccaat
     4681 atcggcaatg tggcagataa ccctgtgggt aacggcttaa ttaaattgga tttaccgttc
     4741 ttagattgga atcgggttaa aaataacgta aaaatttctg aaacggatta tgtgactgcc
     4801 cgtttaaact atgagcaaaa aattacctca gccttaaatg aaattgaccg ttattattac
     4861 agttatacgc agtcgagaag ctcgttaaaa atccttcaag ataccaatgc gaaaaataaa
     4921 cgtatcagtt catactataa aaaccgttat gaacaagggg tgtcggagtt ccgtgagtgg
     4981 attaccgcac tgaataccga gcttaattct cagttggcga ttttaaatgc aaaatatgcg
     5041 attttgacca acgagaatgc ggtttaccaa gcaatggcag gtaaatacag aagataatta
     5101 tatctaaaac tatatactat ggttggtaaa agtgggttaa ggcttgatgg cttaacccgc
     5161 ttcgctattt attgctactt aaaccatttt ggtaatgtta gtttagctac tagaacttaa
     5221 tcacatcacc gtttttcggc acagttacac gatttaatcc ctctttttgt acataatcac
     5281 gcatctgatc actgctgatt gaggtttgtg gcagggcgtc catatgcaca gttaagattt
     5341 tcgcttgtgg agcgattttg tgtgctttgg aaacatcttc ggtacacata atcgggctgc
     5401 cttcaaagtt tggtagtttg gcacagccgg cattcagcac cacaaagtct ggtttgtaag
     5461 tgttgaacga atgttcgatt tcgtggttcc aaatggtatc tcccaccaaa tagacggtcg
     5521 gtttgccttc cgctttcatc actacgtcca tcgcatcgcc gaccatttcg gcaattttcg
     5581 ggttcgcata catcgcatcg gtgccgtgct ggccacgagt tcgggttaag gttacgcctt
     5641 caaacggggt atctttgccg accacacgca catcggtaaa gccttgtgaa cggattaaac
     5701 gggcatcacc ggcattctgc acaaaaatcg gcagattttt cggcacacgt tgttgtgcta
     5761 catcgtccca gtgagcgtgg tgagtgtgag ttacgatcac cgcatccacg ccatttaaca
     5821 cttcatcaat gctcacagtc atatccaagg tcggattacg ttttttcgca ttcggcggta
     5881 agtagcgatc tttctccgct aaatacggat caaccaaaaa cgtcttgccg gcatattcaa
     5941 ttttcgccgt tgcattacga atatgttggt attggctctg ctcagcggca aaacttgatg
     6001 tagcgaaaag tgccgttgca atcaggctca gtgctgtttt tttgacaaaa gtcataggtt
     6061 ctccaaaata aattaaaatg cagagtatga ttttttacgc tctgcaatgc tggtttgcat
     6121 aaggaacagt gtcccttaca tcaataaaag cggtcgattt tgcacatttt tctgcaaaac
     6181 caaccgcttg ttcggctagg ctaatttctc taccttcgcc ggaatgccat ttctcaccgc
     6241 tgagccacag acccaatcta cccacgggga tttgaccgct ttggtgtggt cgataatgcc
     6301 gaggtcgttg aggttaatcc ctttggcgat catcgggttg gctggaattt ccacgccgtc
     6361 aatggtgtag gaacttgccc caatctgttt atgaccgtag ccgtgttcga tgccgattgt
     6421 gcctcgggcg ataccgtcca acaccacaat ctgcaccgtc aagccggcgg ttggggtgct
     6481 gagtttcacc acatcgccgt gtttaatgcc tttttcttcc gcatctaaac ggttcatcgc
     6541 cactaagccc tcaggcttga tcgagagcag acgcaacagt ggggcggtaa tactgctcat
     6601 aatgttcgat ttaaacgaaa tcagtttaaa gccccattcc gactgcggat agtgctgctc
     6661 cagcgtggag ccgtccgcaa aacgtggcga catataacga gggcagcccc aatagtgttc
     6721 accgttgctg tggtgctggg ctttatacac gttttcgttc caaatttgca ggcagttttt
     6781 ccactggaac tccatattct cctctttcca cgcactttcg tgtggggcaa aacgtccgcc
     6841 tttgcaatac acattcgcca cttttaatcg ctcttcggct tttagcgttt gttcaatttt
     6901 cggcataatc cgttgcacgc cggtgagcag cagatcttgc tcgctggcgt ccggtgcagg
     6961 tttctcaccg gcaaaggcaa tgttcgctgc cgcccgtagg aagtaatctt cggtacggtg
     7021 gagcggatag cggttgcctt ctaaatcggc aatcgcattt tcgccaaagc ccggcaagcc
     7081 catttttttc gccaccgcaa tcacgaaatt ctccatacag atggtgtcgc cttcggcagt
     7141 tttggcgttt ttggacggca caatcgggtg gcgtgcggtg ctggtgcgtt gaggcacacc
     7201 gccccacgcc gttgaaaagc cccagctctc aaagttgtgg gtgtccggca cgatgtagtc
     7261 cgtcagtgca gtggtttcgt tcataaaagc atcaatagag acaaagagcg gtagaatttt
     7321 cggatctttt aatttctcca acgcaatcgg gttcatagcg gttaagccgt aaacggggtt
     7381 cgccatatgg ttgatccacg ctttcgcaaa gtatggatag ccctgcacca ttgaggtaaa
     7441 catctcggta gtttgcccgc cggtgaacgg ataccacgcc ccttgtgccg gatacggatt
     7501 ttcgcccttc gtcactttct gcttaaattc gcttgaattt tggtacgcct ttttactgcg
     7561 ggcaaggttc gtgccttttg gcttcacttc attcgggaag ttcgctaaat tgtagcgagg
     7621 cccgtccccg aaatctttga atttgcctgc ggctactgcc attccgcctt ttttgttgag
     7681 gttaccgacc attgcattca gcaacaaaat cgcccaactg gtataaaaac cggtgctgtg
     7741 catcgtgccg ccgtgagtga tggcattggc tttatgaccg tgcgaagtga attttttcgc
     7801 taagttttca atggtttgca ccggcacgcc acaaatgtcg ctatattcgg caagggtttt
     7861 ctcttgtgtc gcctctttca gcaactgcat tgaggatttc accaacactt ccgagccatc
     7921 aagcagtttt gcaatttgtt cgacaaaaat gaccgcttgt aagcaatctt ttgctgaaat
     7981 gagttcgccg cttgcctgat cttttaccac aatcaggttt tcttccactt tctcttcggg
     8041 gtcgagttgc acatcttgca gatcgctaat ccgcaacgcc tgcccgaatt tcgggtggtt
     8101 cggttcgcaa ataatcagat gtgacgcatt ggcatagctg acgtggccgg cattttccat
     8161 cgctgccaga cttgggcggg agaggtagtc agcattgaaa cgctcgtttt cgataatcca
     8221 acgcaacatt cccattacca acgccaaatc gccctcgggc ttaatcggca cccaatggtt
     8281 gttttgggtg gcgagggagg aggtcatctc caaacgaggg gaaaccacca catagctgaa
     8341 attgtcatca gggcggcgtt tggcaagctg gcgagcctga cgcttaaacg gattgcccga
     8401 ctgtgccgga gacgtgccga taaacagaat aaattcagcg tgatcccagt ccggcttggc
     8461 gtgttgttgg ttgtcaaaat ctttcataaa ggcacccgaa ccggcacggt acgcagcgcc
     8521 acaataagcc ccgtggttgg aaaagttgat cgtgccgaag ctcttttggg caaagcgttg
     8581 caaaatcggc tgacgacctt cggggccggc aaaagtaact aaaagctggt ttgctttcat
     8641 tcccaaatca ggctgtttcg ggttaatcgg atcgaccaga ttgcggatcg tcttcaagcc
     8701 ctccacttcg ccttcaccaa ataggttacc accattgatg atttcctcca ccaactgctc
     8761 aaagctgatg gtctgccact tgccttcgcc ccgttttccg acccgtttca acggttgggt
     8821 aatccgatac gggctgttca ccccctccaa gaacgccgcc cctctagcac aggcggtgga
     8881 gcgattttca atgcc
//
LOCUS       DANLUQ010000321         3332 bp    DNA     linear   BCT 23-FEB-2023
DEFINITION  TPA_asm: Mannheimia haemolytica isolate Culture
            SAMN25995846-rid16725373.guided.195, whole genome shotgun sequence.
ACCESSION   DANLUQ010000321 DANLUQ010000000
VERSION     DANLUQ010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25995846
            Sequence Read Archive: SRR18050800
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Mannheimia haemolytica
  ORGANISM  Mannheimia haemolytica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
            Pasteurellaceae; Mannheimia.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-JAN-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001574657.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001574657.1
            Genome Coverage       :: 95x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/12/2023 22:21:49
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,632
            CDSs (total)                      :: 2,580
            Genes (coding)                    :: 2,513
            CDSs (with protein)               :: 2,513
            Genes (RNA)                       :: 52
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 45
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 38 of 67
            Pseudo Genes (incomplete)         :: 18 of 67
            Pseudo Genes (internal stop)      :: 12 of 67
            Pseudo Genes (multiple problems)  :: 1 of 67
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Mannheimia haemolytica"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25995846-rid16725373.guided.195"
                     /isolate="Culture"
                     /host="cow"
                     /db_xref="taxon:75985"
                     /country="USA: WI"
                     /collection_date="2020-10-13"
                     /collected_by="University of Wisconsin Veterinary
                     Diagnostic Laboratory, Madison, WI"
     gene            1..1203
                     /gene="tet(H)"
                     /locus_tag="PXB27_002629"
     CDS             1..1203
                     /gene="tet(H)"
                     /locus_tag="PXB27_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(H)"
                     /protein_id="HDL5347353.1"
                     /translation="MNKSIIIILLITVLDAIGIGLIMPVLPTLLNEFVSENSLATHYG
                     VLLALYATMQVIFAPILGRLSDKYGRKPILLFSLLGAALDYLLMAFSTTLWMLYIGRI
                     IAGITGATGAVCASAMSDVTPAKNRTRYFGFLGGAFGVGLIIGPMLGGLLGDISAHMP
                     FIFAAISHSILLILSLLFFRETQKREALVANRTPENQTASNTVTVFFKKSLYFWLATY
                     FIIQLIGQIPATIWVLFTQYRFDWNTTSIGMSLAVLGVLNIFFQAIVAGKLAQKWGEK
                     TTIMISMSIDMMGCLLLAWIGHVWVILPALICLAAGGMGQPALQGYLSKSVDDNAQGK
                     LQGTLVSLTNITGIIGPLLFAFIYSYSVAYWDGLLWLMGAILYAMLLITAYFHQRKTT
                     PKAVISTP"
     gene            complement(1299..2609)
                     /locus_tag="PXB27_002630"
     CDS             complement(1299..2609)
                     /locus_tag="PXB27_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL5347354.1"
                     /translation="MSNILNWPDYKVLQVSELEHDYQVHAEVSEPPTQCPHCNHPEIV
                     GFGRRDEVIMDTPVHGRRTGIMLNRRRYRCQSCRKTFLEPVPHKDEKRQMTNRLIQYI
                     ERESLRRTFSSVAEDVGVDEKTVRNIFNDYCERLEKTLNFEMPQWLGIDEIHIIKPRC
                     VITNIQQQTIVDMLDNRNKTTVTRYLSKRTDRDLVRYVAMDMWRPYRQAVETMIPDAT
                     VIIDKFHVVRMANESLERARKAIRSALTPQQRRGLMRDRFVLLKRRHELTDAEYMRFS
                     GWTLNYPEIGQAYELKEAFFEIWDCQTRHQAQEAYYSWLRQITPEMKAHYDPLIKAMG
                     NWHDDIFAYFDHPITNAYTESLNNLIRVVNRVGRGYSFEALRAKILFTEGFQKIKKPR
                     YQRQRIPEGAMGRMPFYGVAEAGPSTNYGADISTLVREIEAGRL"
     gene            complement(2609..2911)
                     /locus_tag="PXB27_002631"
     CDS             complement(2609..2911)
                     /locus_tag="PXB27_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347355.1"
                     /translation="MKIKLYFPDESIATIKRMGLRQMSPETLRWTADHPSSSYGMGAL
                     LRGKSGEILDGKSFAAMVHAFGAWIETDSEDTSRRVHNALVTAATGTEESVKVAKE"
     gene            complement(2952..3140)
                     /locus_tag="PXB27_002632"
     CDS             complement(2952..3140)
                     /locus_tag="PXB27_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011200812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL5347356.1"
                     /translation="MSYDANDALNEIEEALSELERVAEDLINNNPNKESELRGQGVHQ
                     ATKHLRFRIRNIRRGEAI"
BASE COUNT          770 a          801 c          798 g          963 t
ORIGIN      
        1 atgaataaat caattattat tatactgctg atcaccgtat tagatgccat tggtatcggg
       61 cttatcatgc cagtactccc tactctatta aatgaatttg tcagtgaaaa ttcactggca
      121 acccattacg gtgtgctatt agcgctctat gctaccatgc aggttatttt tgctcctatt
      181 ctaggacgac tgtctgataa atacggcaga aaacccatct tgctgttttc ccttttaggc
      241 gcggcactcg actatctttt aatggcattc tcaaccacac tttggatgct ctatattggg
      301 cgcatcattg cggggatcac aggcgcaaca ggtgccgtat gtgcatcagc gatgagtgat
      361 gtgactcccg ctaaaaatcg aactcgctat tttggtttct taggtggtgc ttttggtgtt
      421 ggccttatta tcggcccaat gctaggggga ttattaggtg atatcagtgc tcatatgcca
      481 tttatttttg ccgctatttc acactcgata ttattaatac tctctttgct ctttttccga
      541 gaaacacaaa aaagagaagc gcttgttgcc aataggacac ctgaaaacca aactgcctca
      601 aatacagtca ctgttttttt taagaaaagc ctctactttt ggttagcaac ctattttatt
      661 atccagctta tcgggcaaat tcctgccacc atctgggtgc tgtttacaca atatcgtttt
      721 gattggaaca caacttctat cggtatgtct ttggcggttc tgggtgtatt aaatattttc
      781 tttcaggcga ttgtcgctgg gaaattggca caaaaatggg gcgaaaaaac caccattatg
      841 atcagtatgt ctattgatat gatgggctgt ttattattag cgtggatagg ccacgtttgg
      901 gtcatcttac cagcattaat ttgcttagcg gcaggaggta tggggcaacc cgcattacaa
      961 ggttatttat caaaatctgt cgatgataat gcgcaaggga aattacaagg tactctggtg
     1021 agcctaacca atattaccgg gatcattggt ccccttttat ttgcctttat ttatagttat
     1081 agcgtcgctt attgggatgg tctgttatgg ctgatggggg caatacttta tgctatgttg
     1141 cttattaccg cttattttca ccaaagaaaa accacaccta aagctgttat ttcaacccct
     1201 taatactaga aattataaag atcataaaag tcctgtccgc tcttaataac gttgatagga
     1261 ctttatttgg gtatccggat tacagggttg aaacagggct ataaacgccc cgcctcaatc
     1321 tcccgcacca gtgttgatat gtcagcgccg tagttagtgg atggccccgc ttcggcaacg
     1381 ccatagaacg gcatcctgcc catagcgccc tcgggtatcc gttgacgctg atagcgtggc
     1441 ttcttgatct tctggaagcc ctcagtaaac aggatcttgg ctcgcagcgc ttcaaagctg
     1501 tagccacggc caacacgatt aacaacacga atcaggttgt tcagtgactc ggtgtaggcg
     1561 ttggtaatgg ggtggtcgaa gtaagcgaaa atgtcatcgt gccagttgcc catcgctttg
     1621 ataagcgggt catagtgggc tttcatttcc ggcgtaatct gtcgcagcca agagtaatat
     1681 gcctcctgtg cctggtgccg ggtctgacag tcccaaatct cgaaaaaggc ttctttcagt
     1741 tcgtaggctt ggccaatctc agggtaattc aatgtccagc ctgaaaaacg catgtattcg
     1801 gcatctgtca gctcgtgacg gcgtttcagt aggacaaacc tatctcgcat caacccacgg
     1861 cgctgctgtg gcgtcagggc gcttctgatg gcttttctgg cgcgttccag tgattcgttc
     1921 gccatcctga caacgtggaa cttgtcgatg atcaccgtgg cgtcaggaat catggtttct
     1981 actgcttgcc ggtacggcct ccacatatcc attgccacgt agcgcaccag gtcgcggtca
     2041 gtgcgtttgc tcaggtagcg ggtgacggtg gttttattgc ggttatccag catgtccaca
     2101 atggtctgct gctgaatgtt ggtgatcacg cagcggggtt tgatgatgtg gatttcgtcg
     2161 atacccaacc actgcggcat ttcaaaattg agcgtctttt cgaggcgttc acaatagtcg
     2221 ttgaaaatgt tacggacggt cttttcatcg acgcccacat cctcggcaac actggagaac
     2281 gtgcggcgca ggctctcgcg ctcgatgtac tggatcaggc ggttggtcat ctgccgcttt
     2341 tcgtctttgt gcggtacagg ctccagaaac gtctttcggc aagactggca gcgatagcga
     2401 cggcggttaa gcatgatgcc ggtacgcctt ccgtggacgg gtgtatccat gatcacctca
     2461 tcacggcgac caaagccgac gatttcagga tgattgcagt gggggcactg cgtaggcggt
     2521 tcggaaactt cggcgtgtac ctggtaatcg tgctccagtt cggatacctg gagcactttg
     2581 taatcaggcc agttgaggat attcgacatt actcctttgc caccttaacc gattcctcgg
     2641 tgccagtggc agcagtgact agcgcgttat gaactcgacg actggtatct tcactgtctg
     2701 tttcaatcca cgcgccaaag gcatgcacca ttgcagcaaa agacttccca tcaaggattt
     2761 caccagactt gcctctgagc aaagcgccca taccatagct gcttgacgga tgatcggcag
     2821 tccacctgag agtctcggga gacatttggc gaagccccat tcgctttatc gtggcgatag
     2881 actcgtccgg aaaatagagt ttgattttca tgctgacctc gctgaaagcc gcccctaggg
     2941 cggcatgtta gtcaaattgc ttcgccgcgg cggatgttgc gaatacgaaa acggaggtgc
     3001 tttgtcgctt ggtgaacccc ttggccgcgt aattctgatt ctttattcgg gttgttgttg
     3061 attaggtctt cagcaaccct ttcaagctcg ctaagcgcct cttcgatttc attcagcgca
     3121 tcatttgcat cgtatgacat ggcttctatc ctgcaccaag ttcggcggtg cgcccgttgg
     3181 gcttatgccc gagccgttgt tctcagctct tgaatctaat attagggcat ctggcctttt
     3241 ccgtcaaccc taaaatccgg tttatttaca aaattacgtc tagcgcagat caaccgctaa
     3301 atccggatac ccaaaatatc atatataaat ac
//
